LOCUS       DAMEET010000001        91563 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.001, whole genome shotgun sequence.
ACCESSION   DAMEET010000001 DAMEET010000000
VERSION     DAMEET010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 91563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 91563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..91563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.001"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            52..570
                     /locus_tag="PB780_000001"
     CDS             52..570
                     /locus_tag="PB780_000001"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDE0732089.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            698..1381
                     /locus_tag="PB780_000002"
     CDS             698..1381
                     /locus_tag="PB780_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HDE0732090.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1690..1763
                     /locus_tag="PB780_000003"
     tRNA            1690..1763
                     /locus_tag="PB780_000003"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1724..1726,aa:Arg,seq:tct)
     gene            1922..2281
                     /locus_tag="PB780_000004"
     CDS             1922..2281
                     /locus_tag="PB780_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HDE0732091.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2404..4035
                     /locus_tag="PB780_000005"
     CDS             2404..4035
                     /locus_tag="PB780_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HDE0732092.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4097..4738)
                     /locus_tag="PB780_000006"
     CDS             complement(4097..4738)
                     /locus_tag="PB780_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDE0732093.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4924..5000
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            5020..5328
                     /gene="rplU"
                     /locus_tag="PB780_000007"
     CDS             5020..5328
                     /gene="rplU"
                     /locus_tag="PB780_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDE0732094.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5355..5699
                     /locus_tag="PB780_000008"
     CDS             5355..5699
                     /locus_tag="PB780_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HDE0732095.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5712..6005
                     /gene="rpmA"
                     /locus_tag="PB780_000009"
     CDS             5712..6005
                     /gene="rpmA"
                     /locus_tag="PB780_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDE0732096.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6439..9246
                     /locus_tag="PB780_000010"
     CDS             6439..9246
                     /locus_tag="PB780_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDE0732097.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9437..9928
                     /locus_tag="PB780_000011"
     CDS             9437..9928
                     /locus_tag="PB780_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDE0732098.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            10031..11020
                     /gene="manX"
                     /locus_tag="PB780_000012"
     CDS             10031..11020
                     /gene="manX"
                     /locus_tag="PB780_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDE0732099.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11053..11856
                     /locus_tag="PB780_000013"
     CDS             11053..11856
                     /locus_tag="PB780_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HDE0732100.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11877..12791
                     /locus_tag="PB780_000014"
     CDS             11877..12791
                     /locus_tag="PB780_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HDE0732101.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12874..13248
                     /locus_tag="PB780_000015"
     CDS             12874..13248
                     /locus_tag="PB780_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HDE0732102.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13387..13632
                     /locus_tag="PB780_000016"
     CDS             13387..13632
                     /locus_tag="PB780_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDE0732103.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13804..14868
                     /locus_tag="PB780_000017"
     CDS             13804..14868
                     /locus_tag="PB780_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDE0732104.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15114..15539
                     /locus_tag="PB780_000018"
     CDS             15114..15539
                     /locus_tag="PB780_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDE0732105.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15532..15981
                     /gene="nusB"
                     /locus_tag="PB780_000019"
     CDS             15532..15981
                     /gene="nusB"
                     /locus_tag="PB780_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDE0732106.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16078..16932
                     /locus_tag="PB780_000020"
     CDS             16078..16932
                     /locus_tag="PB780_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HDE0732107.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            16933..18270
                     /locus_tag="PB780_000021"
     CDS             16933..18270
                     /locus_tag="PB780_000021"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDE0732108.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18275..18499
                     /locus_tag="PB780_000022"
     CDS             18275..18499
                     /locus_tag="PB780_000022"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDE0732109.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18501..19391
                     /locus_tag="PB780_000023"
     CDS             18501..19391
                     /locus_tag="PB780_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDE0732110.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19400..20218
                     /locus_tag="PB780_000024"
     CDS             19400..20218
                     /locus_tag="PB780_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HDE0732111.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20325..20774
                     /locus_tag="PB780_000025"
     CDS             20325..20774
                     /locus_tag="PB780_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDE0732112.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20792..22474
                     /gene="recN"
                     /locus_tag="PB780_000026"
     CDS             20792..22474
                     /gene="recN"
                     /locus_tag="PB780_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDE0732113.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22529..22645)
                     /locus_tag="PB780_000027"
     CDS             complement(22529..22645)
                     /locus_tag="PB780_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HDE0732114.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22718..23674)
                     /locus_tag="PB780_000028"
     CDS             complement(22718..23674)
                     /locus_tag="PB780_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDE0732115.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23845..24213
                     /locus_tag="PB780_000029"
     CDS             23845..24213
                     /locus_tag="PB780_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HDE0732116.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24383..24814
                     /gene="mraZ"
                     /locus_tag="PB780_000030"
     CDS             24383..24814
                     /gene="mraZ"
                     /locus_tag="PB780_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDE0732117.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24835..25794
                     /gene="rsmH"
                     /locus_tag="PB780_000031"
     CDS             24835..25794
                     /gene="rsmH"
                     /locus_tag="PB780_000031"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDE0732118.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25799..26200
                     /gene="ftsL"
                     /locus_tag="PB780_000032"
     CDS             25799..26200
                     /gene="ftsL"
                     /locus_tag="PB780_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDE0732119.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26201..28393
                     /locus_tag="PB780_000033"
     CDS             26201..28393
                     /locus_tag="PB780_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDE0732120.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGIAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28419..29381
                     /locus_tag="PB780_000034"
     CDS             28419..29381
                     /locus_tag="PB780_000034"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDE0732121.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29395..30756
                     /locus_tag="PB780_000035"
     CDS             29395..30756
                     /locus_tag="PB780_000035"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDE0732122.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30782..31870
                     /gene="murG"
                     /locus_tag="PB780_000036"
     CDS             30782..31870
                     /gene="murG"
                     /locus_tag="PB780_000036"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDE0732123.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31885..33105
                     /locus_tag="PB780_000037"
     CDS             31885..33105
                     /locus_tag="PB780_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HDE0732124.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33261..34586
                     /gene="ftsA"
                     /locus_tag="PB780_000038"
     CDS             33261..34586
                     /gene="ftsA"
                     /locus_tag="PB780_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDE0732125.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34609..35850
                     /gene="ftsZ"
                     /locus_tag="PB780_000039"
     CDS             34609..35850
                     /gene="ftsZ"
                     /locus_tag="PB780_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDE0732126.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35867..36544
                     /locus_tag="PB780_000040"
     CDS             35867..36544
                     /locus_tag="PB780_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDE0732127.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36558..37157
                     /locus_tag="PB780_000041"
     CDS             36558..37157
                     /locus_tag="PB780_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HDE0732128.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37172..37459
                     /locus_tag="PB780_000042"
     CDS             37172..37459
                     /locus_tag="PB780_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDE0732129.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37508..38290
                     /locus_tag="PB780_000043"
     CDS             37508..38290
                     /locus_tag="PB780_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDE0732130.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38311..39018
                     /locus_tag="PB780_000044"
     CDS             38311..39018
                     /locus_tag="PB780_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDE0732131.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39280..42078
                     /gene="ileS"
                     /locus_tag="PB780_000045"
     CDS             39280..42078
                     /gene="ileS"
                     /locus_tag="PB780_000045"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDE0732132.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42127..43653)
                     /locus_tag="PB780_000046"
     CDS             complement(42127..43653)
                     /locus_tag="PB780_000046"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDE0732133.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43668..44315)
                     /locus_tag="PB780_000047"
     CDS             complement(43668..44315)
                     /locus_tag="PB780_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HDE0732134.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44499..44828)
                     /locus_tag="PB780_000048"
     CDS             complement(44499..44828)
                     /locus_tag="PB780_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HDE0732135.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            45005..45733
                     /gene="sfsA"
                     /locus_tag="PB780_000049"
     CDS             45005..45733
                     /gene="sfsA"
                     /locus_tag="PB780_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDE0732136.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45749..46762
                     /locus_tag="PB780_000050"
     CDS             45749..46762
                     /locus_tag="PB780_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDE0732137.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46762..48039
                     /locus_tag="PB780_000051"
     CDS             46762..48039
                     /locus_tag="PB780_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDE0732138.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48102..50804
                     /locus_tag="PB780_000052"
     CDS             48102..50804
                     /locus_tag="PB780_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDE0732139.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            50956..51273
                     /locus_tag="PB780_000053"
     CDS             50956..51273
                     /locus_tag="PB780_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDE0732140.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51303..51623
                     /locus_tag="PB780_000054"
     CDS             51303..51623
                     /locus_tag="PB780_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDE0732141.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51731..53191
                     /locus_tag="PB780_000055"
     CDS             51731..53191
                     /locus_tag="PB780_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDE0732142.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53259..53480
                     /locus_tag="PB780_000056"
     CDS             53259..53480
                     /locus_tag="PB780_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732143.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53511..53693
                     /locus_tag="PB780_000057"
     CDS             53511..53693
                     /locus_tag="PB780_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HDE0732144.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53693..54106
                     /locus_tag="PB780_000058"
     CDS             53693..54106
                     /locus_tag="PB780_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HDE0732145.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54229..55410
                     /locus_tag="PB780_000059"
     CDS             54229..55410
                     /locus_tag="PB780_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDE0732146.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55481..55645)
                     /locus_tag="PB780_000060"
     CDS             complement(55481..55645)
                     /locus_tag="PB780_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732147.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55799..55872
                     /locus_tag="PB780_000061"
     tRNA            55799..55872
                     /locus_tag="PB780_000061"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55833..55835,aa:Arg,seq:cct)
     gene            complement(55941..57080)
                     /locus_tag="PB780_000062"
     CDS             complement(55941..57080)
                     /locus_tag="PB780_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDE0732148.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGLEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(57379..58014)
                     /locus_tag="PB780_000063"
     CDS             complement(57379..58014)
                     /locus_tag="PB780_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732149.1"
                     /translation="MDNRNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(58127..58762)
                     /locus_tag="PB780_000064"
     CDS             complement(58127..58762)
                     /locus_tag="PB780_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HDE0732150.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQIEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(58796..59257)
                     /locus_tag="PB780_000065"
     CDS             complement(58796..59257)
                     /locus_tag="PB780_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HDE0732151.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(59387..59818)
                     /locus_tag="PB780_000066"
     CDS             complement(59387..59818)
                     /locus_tag="PB780_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDE0732152.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(59836..60156)
                     /locus_tag="PB780_000067"
     CDS             complement(59836..60156)
                     /locus_tag="PB780_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDE0732153.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            60455..61231
                     /locus_tag="PB780_000068"
     CDS             60455..61231
                     /locus_tag="PB780_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HDE0732154.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            61246..61449
                     /locus_tag="PB780_000069"
     CDS             61246..61449
                     /locus_tag="PB780_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDE0732155.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            61465..61803
                     /locus_tag="PB780_000070"
     CDS             61465..61803
                     /locus_tag="PB780_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HDE0732156.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            61790..61969
                     /locus_tag="PB780_000071"
     CDS             61790..61969
                     /locus_tag="PB780_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732157.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(62012..62482)
                     /locus_tag="PB780_000072"
     CDS             complement(62012..62482)
                     /locus_tag="PB780_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732158.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            62569..63267
                     /locus_tag="PB780_000073"
     CDS             62569..63267
                     /locus_tag="PB780_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HDE0732159.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            63445..63786
                     /locus_tag="PB780_000074"
     CDS             63445..63786
                     /locus_tag="PB780_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732160.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            63779..64450
                     /locus_tag="PB780_000075"
     CDS             63779..64450
                     /locus_tag="PB780_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HDE0732161.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            64456..65142
                     /locus_tag="PB780_000076"
     CDS             64456..65142
                     /locus_tag="PB780_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732162.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            65145..65894
                     /locus_tag="PB780_000077"
     CDS             65145..65894
                     /locus_tag="PB780_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDE0732163.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            65906..66175
                     /locus_tag="PB780_000078"
     CDS             65906..66175
                     /locus_tag="PB780_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732164.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            66180..66344
                     /locus_tag="PB780_000079"
     CDS             66180..66344
                     /locus_tag="PB780_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732165.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            66337..66639
                     /locus_tag="PB780_000080"
     CDS             66337..66639
                     /locus_tag="PB780_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732166.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            66636..66797
                     /locus_tag="PB780_000081"
     CDS             66636..66797
                     /locus_tag="PB780_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDE0732167.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            66794..67099
                     /locus_tag="PB780_000082"
     CDS             66794..67099
                     /locus_tag="PB780_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732168.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            67099..67455
                     /locus_tag="PB780_000083"
     CDS             67099..67455
                     /locus_tag="PB780_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HDE0732169.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            67442..67660
                     /locus_tag="PB780_000084"
     CDS             67442..67660
                     /locus_tag="PB780_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732170.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            67657..68076
                     /locus_tag="PB780_000085"
     CDS             67657..68076
                     /locus_tag="PB780_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HDE0732171.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            68073..68630
                     /locus_tag="PB780_000086"
     CDS             68073..68630
                     /locus_tag="PB780_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HDE0732172.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            68627..68923
                     /locus_tag="PB780_000087"
     CDS             68627..68923
                     /locus_tag="PB780_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732173.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            69000..69413
                     /locus_tag="PB780_000088"
     CDS             69000..69413
                     /locus_tag="PB780_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HDE0732174.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            69722..69874
                     /locus_tag="PB780_000089"
     CDS             69722..69874
                     /locus_tag="PB780_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732175.1"
                     /translation="MKLSVEGTPEEIQELLQAIGSSKEQKVSVSNSDIDGICNCLNRE
                     KISNPY"
     gene            complement(69871..70146)
                     /locus_tag="PB780_000090"
     CDS             complement(69871..70146)
                     /locus_tag="PB780_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732176.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            70600..70806
                     /locus_tag="PB780_000091"
     CDS             70600..70806
                     /locus_tag="PB780_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732177.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            71002..71169
                     /locus_tag="PB780_000092"
     CDS             71002..71169
                     /locus_tag="PB780_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDE0732178.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            71195..71539
                     /locus_tag="PB780_000093"
     CDS             71195..71539
                     /locus_tag="PB780_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDE0732179.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            71544..71825
                     /locus_tag="PB780_000094"
     CDS             71544..71825
                     /locus_tag="PB780_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732180.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            71928..72242
                     /locus_tag="PB780_000095"
     CDS             71928..72242
                     /locus_tag="PB780_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HDE0732181.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            72220..73914
                     /locus_tag="PB780_000096"
     CDS             72220..73914
                     /locus_tag="PB780_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDE0732182.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            73934..75112
                     /locus_tag="PB780_000097"
     CDS             73934..75112
                     /locus_tag="PB780_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDE0732183.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            75075..75761
                     /locus_tag="PB780_000098"
     CDS             75075..75761
                     /locus_tag="PB780_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDE0732184.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            75761..76921
                     /locus_tag="PB780_000099"
     CDS             75761..76921
                     /locus_tag="PB780_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDE0732185.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            76931..77806
                     /locus_tag="PB780_000100"
     CDS             76931..77806
                     /locus_tag="PB780_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732186.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            77803..78114
                     /locus_tag="PB780_000101"
     CDS             77803..78114
                     /locus_tag="PB780_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732187.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            78104..78457
                     /locus_tag="PB780_000102"
     CDS             78104..78457
                     /locus_tag="PB780_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDE0732188.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            78447..78848
                     /locus_tag="PB780_000103"
     CDS             78447..78848
                     /locus_tag="PB780_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732189.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            78841..79245
                     /locus_tag="PB780_000104"
     CDS             78841..79245
                     /locus_tag="PB780_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732190.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            79257..79865
                     /locus_tag="PB780_000105"
     CDS             79257..79865
                     /locus_tag="PB780_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDE0732191.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            79885..80247
                     /locus_tag="PB780_000106"
     CDS             79885..80247
                     /locus_tag="PB780_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732192.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            80449..83880
                     /locus_tag="PB780_000107"
     CDS             80449..83880
                     /locus_tag="PB780_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDE0732193.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            83931..84668
                     /locus_tag="PB780_000108"
     CDS             83931..84668
                     /locus_tag="PB780_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDE0732194.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            84678..86969
                     /locus_tag="PB780_000109"
     CDS             84678..86969
                     /locus_tag="PB780_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDE0732195.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVDGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            86993..89119
                     /locus_tag="PB780_000110"
     CDS             86993..89119
                     /locus_tag="PB780_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732196.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            89136..89285
                     /locus_tag="PB780_000111"
     CDS             89136..89285
                     /locus_tag="PB780_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732197.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            89282..89728
                     /locus_tag="PB780_000112"
     CDS             89282..89728
                     /locus_tag="PB780_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732198.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            89730..89867
                     /locus_tag="PB780_000113"
     CDS             89730..89867
                     /locus_tag="PB780_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDE0732199.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            89905..90198
                     /locus_tag="PB780_000114"
     CDS             89905..90198
                     /locus_tag="PB780_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732200.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            90195..90419
                     /locus_tag="PB780_000115"
     CDS             90195..90419
                     /locus_tag="PB780_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDE0732201.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            90416..91441
                     /locus_tag="PB780_000116"
     CDS             90416..91441
                     /locus_tag="PB780_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDE0732202.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
BASE COUNT        30970 a        14930 c        19258 g        26405 t
ORIGIN      
        1 atcgttatta tatgattaag aaggattgaa taaagaaaga agttgaaaac gatacctgat
       61 tacaaaaaaa taacttttat aagttctata acgttgatta tatttttaat tcttattctt
      121 ctcgtcgtct tggttccccg aatttttggt ttgactcctt atattattga ggacaaagaa
      181 atgagtccat attatgcaca aggaaatctg atatatgtac gaccgaaaaa tacaaatgag
      241 attttagtcg gagatattat tacatattat gaaaattcag gaaaaaaaat ctcgacgcgt
      301 cgcgtaatag ccgttgaaaa caaccacgaa gatttttata caaaagctga tacaaagact
      361 tatattgagc ctggagtagt aaaaaagaga aatattatcg gtagtcccat ctttcagatt
      421 ccatatattg gactagtcct ttcaaaaacg gcatttgcat ggattcaggg gttattcttt
      481 acgattgctt tttgcctaac aggaatcaca gcatggaata cctttgtaga gttgaaaaag
      541 aagcggagac gaacacagaa atttttgtaa gaatgttaaa aacataaatg tattccagag
      601 atccaacgac tcttctgaac atgaaacacg agaaaacact catgcagctt gtagatttag
      661 tcagttgttc gtataatgtg tgaagaggag gagtagtatg cgcaatgaaa tgatgcatcg
      721 tcctgtagtc aaagaagaac tattggattt tatgcgtacc cagcaaaaaa aattaccggg
      781 tgagctaggc aaactggaag aagaagctca tgtagcagaa gtaccagtta ttccccatga
      841 aacagttgta tttttgaaat ttttacttgg acagcttcag cctgaacgaa tcttggagat
      901 cggagcagct atcggttttt catcaagtgt catggccacg acaatcggag aaaacgctca
      961 tgttacgaca attgatcgtt tcgatgtcat gatcgagaaa gctaaaaaaa actatgaacg
     1021 tctaggactg acagataaag tgactttgct tgaaggtcaa gcagcagata tcttgccgga
     1081 gttgtctggt ccgtatgact ttatttttat ggatagtgca aaatcaaaat atattgaatt
     1141 tttacctgaa tgcttacgtt tgttaagaaa aggtggcgtt ttgatggtag atgacatctt
     1201 ccaaggagga acgatcttgc tcccagatga agagattccc cgtggcaaac gagctatcca
     1261 tcgtaaatta aatgaatttc ttcgtgttgt gatggatcat cccgatttga catcgacaat
     1321 cttgccatta ggtgatggtg tgatcttaat gacaaaggaa tcagaaacaa tcgagttgtg
     1381 atacatgatt atggcagcct gtagataatc gattgaatcg agggctcaat tcgaacttga
     1441 tgtaaaagtt tctatatttg taagtcaatt aaaggataca ggaaatctaa ataaacagga
     1501 gtagcatgaa gtcaatgtag ctcctgtttt caattgataa ccttagaaca gctgaaggag
     1561 aatgtctcgt tttaaaagat tgatttagta gttgggccaa aataattcat tatttgctaa
     1621 ttaaataact atttcgtatt gacagaagca atttaattat ctataattat tgatgttccg
     1681 ctgattcttg tcatagtagc tcagctggat agagcatccg ccttctaagc ggacggtcgg
     1741 gggttcgaat ccctcctgtg acgctaaaaa accgttagaa actgaggttt ctaacggttt
     1801 ttctttgttt tttgtgccaa tattgtttgt tttcaaaggc aaataaaata aaaaaacatc
     1861 cttatcgagt acaattataa ggagcaaaaa aataaaactc atcagaaaag aggaagaaga
     1921 tatgacgata ctatctagaa ttttagtgac tctagttgca attgaatttt tttatatcat
     1981 gtatatcgaa acggtgagaa ctgattctga cacgacaagt agggttttta aaatgagtaa
     2041 agaagagtta agcagaaaat ctgttcaaac attgtttaag aaccaaggag tgtacaatgg
     2101 actattagga gtgggactac tttatggagc ttatctttct tccgcatcta aagaaatcac
     2161 aagtatgcta ttaatatcta ttttttttgt tgcattatat ggcagtttga ctagcgatcg
     2221 ttggatttta ctcaaacaag gtgggctagc tctgttggca ttgctttcta ttttcttata
     2281 aatatagaaa aaataagaat ttatctaaca ggaaactttg tgaaaaatat tactttatct
     2341 catgaaatat gatattctaa tgaatggtat tttagaaaat ttcatgaaaa aggagttaga
     2401 taggtgttca aaaattatgt ttcctcacta aaaaaagaat tttcaggtta tgatctttca
     2461 agatttcaga aagatctttt ggctgggatc acagtagctg ctgtagcatt gcctttagcc
     2521 ttggcttttg gggtatcaag cggagctgat gcggccgcag gtttgattac ggctatcatt
     2581 gccggtatcg ttattggcgg tttatcaggc ggtttttatc aaatatcagg tccgacaggt
     2641 gcgatggcag ctatcttgat gtctattgcg gcaacaaagg gaatgcaagg tgtattgctt
     2701 gctacttttt tagcgggtct atttttactt gcagcaggac ttttccgatt aggaacactg
     2761 acttctttta tcccagcacc cgtaattacg gggtttactt caggtattgc tatcattatt
     2821 gcattaggtc agatcgataa tttatttggt gttcactcgg aaggtacaaa cgttattgaa
     2881 aaactaagta gttatcaaac gataggattt ccgataaaca cagcctcttt gatcatggga
     2941 gggctagtca ttttagggat gtttatctat ccaaaaaaat gggcgaaacg aatgcctagt
     3001 tcgctgctgg ctatcatttt ggcaacagca gttatgctat tgttccattt accagtagca
     3061 actgtgggta agattccaca aacattgatc agtagtaatc gtttgaatat gggagatttt
     3121 agcttttctg ccttgcaaca agtggcagtt ccagctatta gcattgcttt attaggaatg
     3181 attgaaagct tgctatgtgg tgcttcggct ggccgaatgg caaataagcc cttagatagc
     3241 aatcaagaat taatggcgca aggaattgga aatctgttgc ttcctttctt tggcgggatt
     3301 cctgctactg cggccatcgc ccgtacaagc gtggccatca aatctggtgc acaaactaga
     3361 ttagctggta tgatacatgc agttgtctta ctgctctcca tgcttgtttt tgcgccagtg
     3421 atgtcacaga ttccaatgcc agcacttgca ggtgttttga ttgtgacggc ttggcgtatg
     3481 aatgaatggg aaacgatcaa ggagctattt actaataaat attggtcagc agttctattg
     3541 tttatcctta cgatgatttg tacagttatc tttgatttaa gtattgctat cgtgatcggg
     3601 gtgatcggcg gctgtgtatt ttttgttgtc aaaagcgctg ctatcacgat ttcattagaa
     3661 acgatcgatt ggcaaagaat ggggctgcca gaaacggaaa agctgaataa ctggacagta
     3721 gtgtatatta gtggtccact gttttttatg tcttcagaaa aactcaaaaa taaattggaa
     3781 gaactaaatc atcacgatgg tgtcattttc tccatgcgag gcgtaccaag tattgatttg
     3841 acggcacgtg cagtttttga tgaatttcaa gaaaaagcag agcaaagagg acaaacgatt
     3901 atctatactt ccttgcagcc agaagtagaa aaacaacttt ctcacttgtg ggaacataca
     3961 ttcacgcatc aacatcaaac agttgctcat gcgttgacag ctttacatgc gaaatatact
     4021 gcaattagcc attaaaacaa tgaaaaagaa cagacaagac tgtaaactga agattttatg
     4081 gtcttgtctg ttctttttaa tattgctgta aaagtgaagc tagtacttgc cagtcttgat
     4141 ggataacagg tacaagggag cgattgtaga aaatagctgc tggatgaaaa agtggaagaa
     4201 ttcgatagtt tttttctgac cattcgtagg catccttttg ttctgttaat tgtaagacag
     4261 gccctgtata gagttgacca tgatttttgg taaccgtata ttctttgcca agtaaacgtt
     4321 gaagaccaat gtttccaagt gtcgcaatta attttggctg gaccttcatc agttcgtaat
     4381 ccaatattgg tgcatgcgca aatacttcgg aacgagtagg tgtccgattt ggataaacag
     4441 tttctgactg ttgtgtccgt ttattgatcc gatgagtcac gcgatacggg cggctcctta
     4501 cagcactggt gatatacacc tcttctcgtg ttaagccagt tgaagaaagt gcctgcatca
     4561 attcttttcc agcttgaccg ctaaaaggaa catgcgtatc aatctctgtt ttccctgggg
     4621 cttctcccac gagcatcagt ttaggatgaa gaggtccttg cccagcgata aatccttcta
     4681 caggacggcc gttcattctt aaagaagctt cttgtatcag ttcttttggg tattccattt
     4741 atgctcctcc tttttctttt attataagaa taaaagaaac acaatgcgac tattttattt
     4801 ggtagaactg tgaggataga aaagcagaag tattgcttga atttttgttt tactttttga
     4861 ttttaaattt agttgactaa cggttgctgt tctggtaagc tagtactgtt gtaattgtgg
     4921 actccacagc tacaaccgct cagaacaagt aataagcaca gcatatgctg tcacttcgga
     4981 tggcgagtct aagtatatca gaaggaggtg ctttatagca tgtacgcaat tattaaaact
     5041 ggtggtaaac aagtaaaagt tgaagtggga caagcaatct atgttgaaaa acttgacgtt
     5101 gaagcaggag aaaaagttgt ttttgacgaa gttatcttag taggcggaga atctacaaaa
     5161 gtaggcgctc caactgttgc aggtgcaact gttgaaggta ctgttgaaaa acacggcaaa
     5221 caaaagaaag tcgttacttt caaatacaaa cctaaaaaac attcacaccg taaacaaggt
     5281 catcgtcaac catatacaaa agtggttatc gacgcgatca acgcttaata gcgtttgaga
     5341 aagaaggcct atatatgatc aaaggaacat ttaagcgaaa tgacgcaggt cagatcgttt
     5401 cattcacttt gaccgggcat gctgatgctg gtccttatgg aagtgatatc gtttgtgcgg
     5461 gtgtttctgc gttagcgatc agcactgtca acgggattgc ctcattagcc ggttttgagc
     5521 cgattgttga aatgaatgaa gaagaaggcg gttatcttta taccgaagtc acttcaggaa
     5581 tgacacaaga acaaaacaat attgctgaga ttctattaga aaatctttta ttaggattac
     5641 aatcgataga agctgaaaac tcagaatata tccaaattaa aacaattaat gataaatagg
     5701 aggtgcagac catgttgtta tctatgaatc tacaattatt tgcccacaaa aaaggtggcg
     5761 gttctacatc taacggccgt gactcagaat caaaacgtct aggtgctaaa cgtgctgatg
     5821 gacaaacagt tacaggtgga tcaattcttt accgtcaacg cggtacaaaa atctatccag
     5881 gtgtaaacgt gggtatcggt ggagacgaca ctttatttgc aaaagttgat ggcgtagtac
     5941 gtttcgaacg taaaggccgt aacaaaaaac aagtgtctgt ttatccagta gctcaagaag
     6001 cttaattgaa tcttatgatt aaagaggttg tgacaagact tttgtcacag ccttttttgt
     6061 ttataaaaat aaaagttgta gtggaggcta cgaagtttac tttgtctttt tacacttaat
     6121 atttttttgc caatttggaa tcaggatgtt ttgataacta tcgttttcaa ttgatggaag
     6181 ctatattgtt tgctatcagt gaaataatta tatggtaaac agtgttcttt ttcttgtttc
     6241 cattataaaa atcgtatcgg gatttttcgt tcttttaaat tgaacatttc tatctatccg
     6301 cgtgttatca acaaaacaaa atcaggtaaa tagttgacga aacagaaata aatacatagt
     6361 attatgtttt gaagcctaag tacaatagtc tatagaaaag gagcgatacc attggcaaaa
     6421 cgcattgacc gaatttatat gtatgtcaaa gaaaaaaccg ctcatttaac aaaggcagaa
     6481 tatgatcaag gagtaacaac acaggaaatt gctgaagtgt tagggattca gcgaacaaat
     6541 agcagtaaag atctgaatca gctagtgcgc gaaggaaaac tgataaagac agacggacga
     6601 ccagtacgtt acatatatca aacactcgtt acgcacgtaa agccagttac aaaacatgta
     6661 gtcagttaca aagaaacgcc ggtctctgaa gaaaaaaata cactccaaat agatactaag
     6721 gatatctttg caaaaatcat tggagctaac ggcagcatga aaaattcagt ggaacaggca
     6781 aaagctgcga ttttgtatcc gccaagagga ttaaattgtt tgattacagg acctaccgga
     6841 tcaggaaaga cttattttgc acacgcgatg tatcattttg caaaagcgca tcatgtaata
     6901 gatgaggaaa cggaactgat tgttttcaat tgtgctgatt atgccaataa ccctgagctt
     6961 ttgatgagtc atttatttgg gtacgcaaaa ggtgctttta caggcgcaga tgaagaaaaa
     7021 atggggatta tcgatcaagc ggatggcggt atgctgtttt tggatgagat tcaccggttg
     7081 ccgcctgaag gtcaagaaat gatcttttac tttatggatc atggaactta cagtcgtctt
     7141 ggggaaacaa cgaaatccca tgaagcaaat gtacggatcg taggtgcgac cacagaagac
     7201 ccaggttctt ctctattaga aacttttgtt cggcgtatcc caatcaatat caaacttccg
     7261 gcttttgaaa aacgtcctgc aaatgagaaa gtcgatttag taaaaatcat gattgcccac
     7321 gaagcaaacc gcacgcagcg aaagatttct ttgacagaag atgtagcaaa agcattgatc
     7381 ggcagtgtga cctatggaaa tataggacaa ttgaagtcaa atatccagtt agtatgtgca
     7441 cgaggatttc taaatcatat gcaatcaccc gaaatctcga tcacaatcga tgatttgaca
     7501 gaagggattc gtagcggatt gattcaatta gcaagcaacc gcagtgcaat ggcagagctt
     7561 tctaaaatat tggagccaaa gataactgta gcaccaaatg atacgatcat gaaaatccaa
     7621 tcggactcct atgagttacc ttataatcta tatgatatca tcggagataa agcagcttta
     7681 ctgaagtcgg atggtttgga tcaagaagca atcaatcatt tcatttctac agatatcaac
     7741 atccatctga agtctttcta taaagaccat ggtttttctt ttaatgcaga cagcaaatta
     7801 gcagaatttg tagatccgaa aatcatagaa gtgaccaatc agatttatgt aatggtcaaa
     7861 agatctttac catatgagtt tcagcaaaac tttatttatg ctatgagctt gcatatcagt
     7921 tcttttttga aaaaaatcaa cttaggagaa gaacggcata cgaatgataa catccgtgag
     7981 atggcttttg attttccaga tgaatatgct gttgcgaaag aagtgcgtag ttatatcgaa
     8041 acgtattttc aagtggagat tccagaaagt gaagactatt acttgaccgt tctactcgtc
     8101 tcgttacggg caaatcaagc aagcggcaga atcggtgtag tcgttgcagc tcacggaaac
     8161 agcacagcaa gcagtatggt ccaagttgtc caacagttgt tagatgcaga gaacgtacga
     8221 gcagtagaca tgcctttgga tatggatccg aaaacagcat tggaccgaat tgaacgcaat
     8281 gtacaagaag tagatgaagg aagcggggtc atactcttag tagatatggg gtcccttgcg
     8341 tcgtttaatt cgcaaattca aagagacact ggaattccag tacgtactgt tgatatggta
     8401 acaacttctc tagttttgga aactgtacga aaggtctcag tattagggac agatctagat
     8461 atgttgcatg aatcattgaa aaattttcga ggttacgcag aaatatcttc tgtagaatct
     8521 gttcaggaag caaaacctat tcataagaaa aaagctgttt tggctatctg cgcatctggc
     8581 gaaggtacag cacaaagaat caaagaattg atcgaacgag cggtaaacaa gcgtcaagaa
     8641 acagaattag aagttctagc tttgtcgatt gtggaagtga aagaagaact gccagttatc
     8701 caaaaagaat atcagatcat agctactact gggattactg atttgaatat ccaagcccca
     8761 tttattcctt tagaacgttt tattgatcaa aatatcgaag tcattttgga tcagttgctg
     8821 atggaatcag aattggagga aactgaattc atctccctag atgaagagtc agctaaaaat
     8881 acgtgtgttg agtttatctc tgataacttc atctttatta atggtagtaa gttaatcgat
     8941 ccaatgtggc aattttctac acaaatctct cagacgacgg gaatcggcga tgaagaatat
     9001 ggattcaaaa tcaacctagt gatgcacaca gcaggaatga tcgaacgtat tattcggaat
     9061 gaaccattga ctgtggaaga aaacgaattg acaaatacaa cgaatgatcc tctttatagc
     9121 caattggcag cctcagttgt tctgttggaa gatcagataa aagtaaaagt gccgatagaa
     9181 gaaatgtatt atctgctacg tctagtccat aatcagttag ataaaaaaga atacactgtg
     9241 ccatgaatac acaaaaacga tacactttta tagtgtatcg tttttgtgta ttggttaaga
     9301 atcctttaaa ttcaattagt gtatcgtaaa aaagttggca cgctttttgc ttatagtagt
     9361 gtgtgctagg aggtggttgt tcacacagaa atgatagggg tttctgtgaa gtagtgagga
     9421 agaaggagtt ttgcagatgg atattcgatt ggcacgtata gatgatcgtt taatccacgg
     9481 gcaagtcgca acagtttggt cgaagcaaac aggtgttgaa cgtatcattg tgatcagcga
     9541 tgtagtcgct caagataaat tgagaaagtt tctactaaaa gaagctgcac cgccggggat
     9601 cacagcaaat gtaataaccg tgcagaaaat gattcaaatc tatcatagtg gattattatc
     9661 acgagcaaaa gtgatgcttt tattcaccaa tccccaagat gttgaagcgg ttgtccgctg
     9721 cggtgtacag ttgaagtcgt tgaatatcgg cggtatgagt tttactgaag gcaaatcaat
     9781 gattaccaat tttgtttcag taaatcaaca agatattgat tcctttcgtt acttgaacag
     9841 tcaagaagtg gagttagaga ttcgaaaagt acctgctgac aggaaaatcc ttttgatgga
     9901 actactagcg aaaatcaaaa aggtgtagca agaatggttg ttggaaaagg tcaaataatt
     9961 ctgttgtaat ggttagcaat tattaatatt tttgaatttg ttacaacaaa agtaaatagg
    10021 aggtataaat atggtaggga ttatcctagc aagtcatgga caatttgctg aaggaatctt
    10081 acaatccggt tcaatgatct ttggcgaaca agaaaacgtc aaagccgtga ttttgaaacc
    10141 aagtgaaggt ccagatgact tgagagccaa attggaagaa gcggttgctt cttttgataa
    10201 tcaagacgaa gtgttattct tagttgattt atggggcgga acaccgttca atcaatcaaa
    10261 cacattattc gaagagcaca aagataaatg ggcaatcgtc agcggtttga atttaccaat
    10321 gttgattgaa gcctacgcat cacgcttttc aatggaatct gctcatgaaa ttgcagcaca
    10381 catcattgaa acagcaaaag acggtgtaaa agtaaaaccg gaagaattag aaccggcaga
    10441 agcaccgaaa gcagcagttg aagacgcaca acctaaagga gcacttccag aaggaactgt
    10501 ggtcggagac ggaaaaatca aatatgtatt agcacgtgtt gactcacgtc tgttacatgg
    10561 acaagttgcg acagcttgga caaaagcagt gcaaccaaac cgtattatcg ttgtttcaga
    10621 tgcagtatca aaggatgatc tgcgtaagag actaatcgaa caagctgctc ctccaggtgt
    10681 aaaagcaaat gttattccta ttagcaaaat gatcgaagta gcaaaagacc ctcgttttgg
    10741 aaataccaaa gcattgcttt tatttgaaaa tcctgaagac gtattgacag ctgttgaagg
    10801 tggcgtagac atcaaagaat taaatgttgg gtcaatggcg cattcggtcg gtaaagtcgt
    10861 tgtaagtaaa gtattgtcta tgggacaaga agacgttgaa gcgtttgaga agcttgaaca
    10921 aaaaggtgtg aaattcgatg tacgtaaagt acctaatgat tcacgcgata acatggatga
    10981 tattttgaaa aaagcaaaag ctgaattagc aaaagcgtaa taaataatat taaaatttat
    11041 aggaggctta tcatgtctat tatttcaata attttagtcg tattagttgc ctttctagct
    11101 ggtatggaag gtattcttga tgaatttcaa tttcatcaac cactagtggc atgtacattg
    11161 atcggtttag ttaccggtaa cctcgaagct ggtatcgtac ttggcggtac gcttcagatg
    11221 atcgctttag gttgggcaaa tatcggtgcc gctgtagcac cagatgctgc attagcatca
    11281 gtagcatcag caattatttt agttttaggt ggacaaggtg ttaaaggtgt tccttctgcg
    11341 atcgcgattg ccgttccttt ggctgttgca gggttgttct taacaatgat cgtacgtaca
    11401 atcgctgttc caatcgttca tttgatggat gcggctgctg aagaaggaaa catccgtaag
    11461 gttgaaatgt ggcacatcat tgctgtatgt ttacaagggg ttcgtatcgc ggtcccagca
    11521 gcagcgttgc tatttatccc agcacaaact gtgcaatcat tccttgaatc aatgccagca
    11581 tggttaacag atggtatggc tatcggtggg ggaatggtcg tagctgtagg ttacgctttg
    11641 gtaatcaaca tgatggctac aaaagaagta tggccattct tcgttattgg tttcgttgta
    11701 gctgcaattt ctcaattaac attgattgct ctaggtgctt taggtgttgc attagctctt
    11761 atttacttga acctttctaa aatgggtggt tcatcaaata atggaggcgg aagcaactct
    11821 ggtgaccctt taggcgacat tttgaatgac tattaatctt gaaggaggaa aataaaatgg
    11881 cagaagaaaa aatcaaatta tcaaaaaaag atcgtttagc tgttgcgtgg cgctctacct
    11941 tcattcaagg ttcatggaac tacgaacgta tgcaaaacgg tggctgggca ttttctatga
    12001 ttccagcaat caaaaaatta tacaaaacaa aagaagaacg ttctgcagca ttaaaacgtc
    12061 acttggaatt ctttaataca cacccataca ttgcttctcc aattttaggg gttacattgg
    12121 ctctggaaga agaacgtgcg aatggcgcgc ctgttgatga tgtagcgatt caaggggtta
    12181 aagtaggtat gatgggtcct ttagctggtg ttggtgaccc agtgttctgg tttacagtac
    12241 gtccaatgct aggtgcacta ggtgcttcat tagctatggg aggaaatatc cttggaccaa
    12301 tcatcttctt cttaggttgg aacattattc gttgggcatt catgtggtat acacaagaat
    12361 ttggctacaa agctggttct aaaattactg atgacctttc tggcggattg ttacaagacg
    12421 taacaaaagg tgcttcaatt ttagggatgt tcgtattagc tgcccttgta caaagatggg
    12481 tatcaatcaa attcttacca gttgtatcaa ctgtaaaatt agataaaggt gcttacatcg
    12541 aatgggataa attgccttca ggtggtgaag gaatcaagag tgcatttgaa caagttaata
    12601 atggcttagc cctttctcct gaaaaggtaa caacattaca agacaactta gaccaattga
    12661 ttcctggttt agctgcatta ggattaacat tgttatgtat gtggttgctt aagaaaaagg
    12721 tttctccaat cgttatcatc cttggtttat tcgtagtcgg tgttgttgga cacgtcatcg
    12781 gacttttata atccataaaa atatagctcg ggcgttgtcc cgagcttttt ttatatagta
    12841 tactgttcat aaacaaatat ctggagggag aaaatggttc aatcaatcaa tacaaaagta
    12901 gacttggtaa ttgatgctac ttcgtttacg ggtctgacag attacgggca aatcatgata
    12961 ggtgataatg gttttgagtt ttaccatgcg cgagatagac gcaaatatat ccaaattcca
    13021 tgggaagaag tggattatgt actagcatca gtcatgctaa agggaaaatg gattcctcga
    13081 tatgccattc aaacgaaaaa aaatggtaca tacacatttt cttccaaaga acctaaaaaa
    13141 gtgcttcgtg cgatcagaga atatgtagat gaagatagaa tggtacaatc attgagtttc
    13201 tttgatgtag tgaaacgtgg tattcgtagt atgtttaaac gcaagtaaaa aataaagaaa
    13261 acttctaaga ggaggttttc tttatttttt tactttgcag aagaaaatat gaaatagcaa
    13321 aaccaattat ttgtcccctt taaatcccat atgctaaaat tgattagcaa agaaggaggt
    13381 tatattatga gttggttatg gacattgatt gttggagccg tgatcggagc aattgcaggt
    13441 gcgatcacca gtaaaggaaa ttcgatgggc tgtgtaacga atatcattgc tggactagtc
    13501 ggttcaagta ttggacaagc gattttcggc gaatggggac caaaagctgc aggtatggca
    13561 cttattccat ctattttagg cgcagtgatt ttggtagcag ttgtctcatt tttctttgga
    13621 aaacgatcat aagtttttac aaaatagtaa actaaaagga gttgcagcga gactgtgact
    13681 cctttttggc tttatttatt taaaataaaa agaagttttc cggaaatttt tatttaactg
    13741 tcgtgtacga agaaaagttt gctataatgg aaaaaagcgt gcaaagaaag agggagtttc
    13801 attatgatgc tacgtgtaga aaaattgcgc aaaaaaatgc aagaagaaaa tttggattca
    13861 tttttggtta ccagtccgta caatcttcgt tacttaacga actttacagg tacgacagga
    13921 ttagctgtta ttactttgga aaaagcattt tttattactg attttcgtta taccgagcag
    13981 gcggcagcgc aggctcaagg gtttgaaatt attaaaaatg tcggacctat ttttgatgag
    14041 gtagcaaact tagtacgcaa agaagaactg cactcattag cttttgagga aacaacagtc
    14101 tcatttttag aatattcggt tttagaagaa atcatcgatg cggaattagt acctgtttca
    14161 cagatgatcg aagaactcag agaagtgaaa gatgaagaag aaatcgctat tatcgaaaaa
    14221 gcatgtcata ttgcggatat ggcatatgat cacatcctga aaatgatcca accgggcatg
    14281 actgagattg aagtcgcaaa tcaactagac ttttatatgc gttctcttgg cgcttcaggt
    14341 gtttcttttg atacgatcgt tgctagcgga ttgcgttcgg ctatgccgca tggtgtagca
    14401 agcaaaaaaa tcatcgaaca aggtgacttg atcacattgg actttggttg ctattacgaa
    14461 gggtacgttt ccgatatgac acgtacattc gctattggtg atcctgggga aaaattaaaa
    14521 gaaatttatc aaatagtctt agatgctcaa ttagcagtta ttgatgctgc aaaaccagga
    14581 atgactggtg tccaattaga tgcagttgct agagattata tttcaaaaca tggttacggg
    14641 gaagcatttg gacattctac aggacatggg atcggtttag agatccacga aggaccaaac
    14701 gtttcttctc gtgcagaaaa gcaatttgta ccaggaaatg tgatcactga tgaaccaggg
    14761 atctacttac caggaatcgg tggagtacgt attgaggatg acttgttgat cacagaagat
    14821 ggaaatcgtg tgttgactcg ttcaccaaaa gaattgatca tcttatagtt tcatcaatag
    14881 aagaagtaag cgtaaaaatc agtgttcaca gactttgtta tagaaaatca ctcgatttaa
    14941 tgatacacta tagaagaact tttaagctgt aagagataat aaagttgggt gttttctcca
    15001 attcatagca gtttgcagct gatcggcggc agttttctta ccacgtgatc actatgccgc
    15061 atcatagaga attcggatgt atctcgtgct taggcaaagg gaggaataaa taaatggcag
    15121 atgaaaaaaa tttgatttta aacgcaaacc aagatttagg agagatcgtg atcgctcctg
    15181 aagtcatcga agtaattatc gggattgcag cttctaaagt agaaggcgta tacggaatgc
    15241 gcggaacttt tgccagcaat gtcactgaat tattaggacg tgcagctcac ggaaaaggtg
    15301 tttacttagc aaacgaggaa gatggcttga aagtagatct ttactgttat ctagaatatg
    15361 gtgtttcagt tcctaaagta gcaatggaaa tgcaggaacg ggtcagccag caagtccttt
    15421 tcatgacgga tatcgattta tctgaagtca atatccatgt agtagctgtt gttcctgaaa
    15481 aattagaaca accaacaatc gaagaacttt ttgaagacga agaggaagag aatgaataaa
    15541 gaattatcaa gacacgagat tcgggaaatg gcattgcagg ctttatttcc attagacttc
    15601 aatgcagacc taacaaaaga agatgcgatt ttcaacgcaa tcgaattaga ccaccgcgac
    15661 atgatcaacg aagatgaatc agagtttgtt cctgtttatc ttgatacgct agttggcggt
    15721 gtctgtgcaa aaaaagatga attggataaa gtgattgaaa aacatttaaa aaacaattgg
    15781 cacatcaatc ggatctcaaa aatggatttg gtgatcttgc gtatcgcgat ttttgaaatg
    15841 acttatgtga cagatgtacc agcggctgtt gctttaaatg aagcaatcga gttggcaaaa
    15901 acatttagtg atgaccgttc acgtaaattc gtcaacggtg ttttatccaa tgtattgaaa
    15961 gaattagaag ctggggcgta agtcctagct tttttctatg aatcatcata aacctgttat
    16021 aaatcgggaa aattctatgg tagaatgaaa acacaaaaaa tatctgggag tggttagatg
    16081 gcagaattga ttaacggaaa agaattagct gaaaaaatgc aggcagagat tgctgaaaaa
    16141 atccaaaaat tgaaagataa tggtatccat cccggattag tcgttttatt ggtaggggaa
    16201 aatccagcca gccaaatcta tgtaagaaat aaagaacgtg tagcaaaaga aatcggaatc
    16261 cattcattgg tagagcgtta tcctgagacg atttctgaag acgaactact tgcagaaatt
    16321 gaaaaataca atcaagatcc tagttttcat ggtatattgg tccaattacc attgccaaag
    16381 catatagatg aagaaaagat cctgttagct atccgccctg aaaaagacgt agacggtttt
    16441 catccgatga atttaggcag actccttgcg ggtaaaccgg acaagattcc ttgtactcca
    16501 tacggcatca tgaaaatgtt tgaagcttat tctatcgatc cggcgggtaa acgagcagta
    16561 gttatcggac gaagcaatat cgtcggtaaa ccgatggctc agctactctt gatggcagat
    16621 gcaactgtta ctgttgctca ttctaaaacc gaaaatctgg ctgaactggc acaagaagca
    16681 gatattttag tcgtagcaat aggacgtgga cattttgtca caaaagaatt cgtaaaacca
    16741 ggtgctgtag tgatagatgt agggatgaat cgtgatgcta acggtaagct gatcggtgat
    16801 gtgaaatttg atgaagtgga gcctattgca ggctggatca ctcctgtgcc aaagggtgta
    16861 gggccaatga ctatcacgat gctgatgtat caaaccgtaa aatctgccga agcttttgga
    16921 gcaggtgaat agatggcaca agaatattta actgtcacta cattaacgaa gtatttgaaa
    16981 agaaaatttg atgccgatcc ttatttagaa cgcgtatatt taacaggcga aatatcgaat
    17041 ttccgtttac gtgccaatgc ccatcaatat ttcagcttaa aagatgacca tgcaaaaata
    17101 tctgctatta tgttcaaagg agcattccaa aaattacgat tccagccaaa agaaggaatg
    17161 aaagtccttg tcgttgggcg tatttcatta tatgaagcgg gaggttctta tcaaatctat
    17221 gtagagcaca tggagcctga tggaataggt gctttatatc aagaattgga agaacgaaaa
    17281 gaaaagctga tgaaggaagg cctttttcaa gggcctaaaa aaatgctgcc acgtttccca
    17341 aagcgaatcg cggtccttac cagtccaagc ggagcggtta tccgcgatat catcacgacg
    17401 gtgaaaagaa gatatccaat tgtccaattg gttttgttcc caacagttgt acaaggaaac
    17461 caagcagcgg acaatgttgt caggaatatc cagagagtag aagagatggg caatttcgat
    17521 acaatgatca tcggtcgtgg gggtggttcg attgaggatt tatggccgtt taatgaagag
    17581 cgggttgctc gggcgattta tcaagctaat acgccggtga tttcatctgt aggacatgaa
    17641 acagatacca cgatcgctga tttagtagca gatgttcggg ctgccactcc aacagcagct
    17701 gcagaattag ctgttcctgt tttgtcagaa gagttgatgc gcattcaaga acggcaagct
    17761 agattgcagc aggcgtttgt acgccagatc cagcgcaaac aagaacggtt cgagcgtgct
    17821 cgacgttcct atgtatttcg gcagcctgaa agattgtatg aaggtcagac catcaaatta
    17881 gaccagttga aacagcgatt atatcaagct acacagcaaa tttatcatgc gaaagaaaaa
    17941 caaacgatcg cattaagcca ccaattggaa caagtggctc cgctttatcg agttaaagca
    18001 gctcgccaag aaactgctta tctagaaaaa cgcttagaag agaaaatcca acaatatatg
    18061 aaacaacagc agcagcgttt tgctcaagct gttcaatctc ttgatttgtt gagtccattg
    18121 aaaatcatgg gaagaggcta cagctatacg acgttaaatg atcaagtagt aaaaagtgtc
    18181 accgagattc atcaagggga tttgctgacg atccattatc aagatggaat cgttaaagga
    18241 caagtacaag agatagacaa aggagaataa aaatatggaa aatccaactt ttgaagagtc
    18301 actgcaagaa ttagaaaaaa ttgtcatgca gttagaacaa ggagacgttc cattagaatc
    18361 agctctagat tcgtttaaac gaggaatgga attaagcaaa cattgccaag atacattagc
    18421 taaagcagaa gagaccttga cgaaaatgat gacagaatca aatgaagaag ttgattttga
    18481 cggaaacgag gaaagctgag atgagattaa ctgacttttc agctattcat ctacctgctg
    18541 tagaaaaaga aatcttgtct tttttagatg agcatacaac ggatagagga ctctatgatg
    18601 cgatgagtta ttcagtgaaa gcaggcggga aaagaatacg tcccttatta cttttaacgg
    18661 ccgttgcttc ctttgatgag cctattgatg ttcccgtcta tcaagtagca gctgctttag
    18721 aaatggtgca tacgtattcc ttgatccacg atgatttgcc tgcgatggac aatgatgatc
    18781 ttcgtagagg aaaacctacc aatcataaag tattcggcga agcattagct atattggccg
    18841 gagatggttt gcttacagga gcattccagc taatcagtat ggcacactta ggtaattctc
    18901 ctaagcttct gctgcttcag caattagctg tttgtgcggg atcacaaggt atggttgctg
    18961 ggcaagctgc agatatcgag ggcgaatcga aaaagctttc tttagaagaa ttagcattta
    19021 ttcatgaaag aaagacaggg catttgattc gttatgcctt gctggctggt ggcatattag
    19081 cgaagcaacc tgaagaaatc ttattgctac ttcaacgatt agccgaacat ttaggtttag
    19141 cttttcagat aagagatgat ctactcgatg tgattggtac aacgaaaaca ttagggaaaa
    19201 cagctggtaa agatgaacgt atggaaaaaa atacttaccc gcgtttatta ggcctagaaa
    19261 agacacgtga ggcgttggag attgagctgt tatccgccaa caagatcata gacaaattag
    19321 aagaggacgt tttttctttt gatggcgggc tgatgcgcca gatgatcaag caatttgatg
    19381 tggagaaatg agggaaagaa tggaaaaaga acgcgtagat gtattagcgg tcaaacaagg
    19441 attatttgaa acaagagaac aagccaaacg ttcagtgatg gctggtttga tttacaacga
    19501 aaaaaacgag cggtttgata aaccgggaga aaaaattcca gtgagtagtg aattaagagt
    19561 caaaggtaaa aaacttcctt atgtttctcg agggggatta aaattagaaa aagcactcaa
    19621 agcatttgat ctatctgttg aaggaaaaac attgttagat attggtgcat ccactggcgg
    19681 atttactgat gcagctttgc aaaatggcgc aaaaatgagt tatgctttag acgttggcta
    19741 caatcagcta gcttggaaaa taagacaaga tcctagagtc gtcgtaatgg aaagagtgaa
    19801 ttttcgttat gcaaaacccg aagactttac cttaggaatt ccagaagttg ctgttatcga
    19861 tgtttctttt atttcactaa aactgatgct gcctccatta catgctattt taaaagaaga
    19921 gggagaagtg attgctttaa tcaaacccca attcgaagca ggcagagaag ctgtagggaa
    19981 aaacggaatt gttcgtgacc ctcagaccca tcaaaaagtt ttagaagaca ttttgtcatt
    20041 tgcggcacaa ggtggatatg atgtgctgga attaagttat tctccgatca cggggggaga
    20101 aggaaatatc gagttcttgg ctcacttgag gaaagtaccc gaaagcggaa caatcaattc
    20161 agctattaat atggcagaag tggtttcaaa tgcacacgaa cagtttgacc ataaataaat
    20221 tgtaactgtg aaaatcaagt gagaagtatg cggattcttc tttattatac caaggaaata
    20281 agctatcatt gattaagaag aatatgctaa ggagcgaaga cgtaatgaga aaaaaagatc
    20341 ggcatcgttt gattactcga ttgttgagtg agcaagatat ccgaaagcag gaagaatttg
    20401 ttgaaatcct gaaaagcaaa ggaatatctg ttactcaagc tacgatttcc agagatataa
    20461 aggaactgaa actgatcaag gtaccggctt cagatggcgg ctaccgttat agcataccag
    20521 ctgaaacaaa cgaagatgta aatactaaat tggaaaaact attaaaagac gctttcgtgg
    20581 cagttgacca aatggaaaaa tttgttattt tgaaaacatt gccgggaaat gcttcggcag
    20641 cagcaaattt gattgataaa cgttacaaga aagaattatt ttcgattatc aatgatgacg
    20701 ataatgtttt gatgattaca cgaactgaag accaggcagt tttattgaag aaagacttcc
    20761 tgcattacct ttaagttgag gagcgaatgt gatgctacaa gaaatcagta ttactaactt
    20821 tgcgattatt ccggaattgc ggctttcttt tcacgaagga atgaccgcgc tgaccggaga
    20881 gactggtgcg ggtaaatcca ttattatcga tgctttaggt ctgttagcag gaggaagagg
    20941 ttcaagcgat tatatacgtc aaggagcaga aaaatgtgtg cttgaagggt tgtttgaact
    21001 accaaaacaa gaaggattct ctgaactgat ggttgaatta gggatcgaaa cagatgagga
    21061 caatctgatt gttcgacggg atatgtcctt aactggaaaa aatgtttgtc gagtgaatgg
    21121 tcatattatc acattagcta atctgagaag aatcggcagt tatttagtag atatccaagg
    21181 acaaaacgaa catcaggaac tgctccagcc tgaatcacat cttgctttat tggatcgttt
    21241 tggagatgta gctttccaac agaagaaaaa gagttatcag caagaatata tctcttatcg
    21301 tgagctggaa aaaagggtac gaaaagtgca gcaaaatgaa aaatcctatg ttcagagaat
    21361 agatatgctc catttccagc aagaagaaat agctgctgct gatttgcaag tcggagaaga
    21421 agagaaatta aaagaagaaa gagaaaaact tagcaattat caaaaaattg ctgatggatt
    21481 agctgcaggt tacggagcac tgacgaataa tgaacaaaac agtgtagatg gtgttggctt
    21541 ggccgtttca gaactgcaag ggatcgccca tctagatgtg gagtatgaag caatttatga
    21601 aaatatccaa agtgcttact atttgctgca agatgcaatc ggcgatatga gtcgtcagat
    21661 tgatttattg gagttagatg agagtcgttt agaagaagtt acgcaacgtt tagaattaat
    21721 ccgacagttg aaacgtaaat acggcgaatc cattgagtca atattggctt actatgacga
    21781 aatcaccgaa gagctagctt cttctgattt ttcagaaggt caattggaca aaatgaaaga
    21841 ggaactagag caaaaagagc ttctacttca gcaacaagcc gcagatcttc atgaagcgcg
    21901 taaaaaaatc gcaaaagaat tggaaaaatc cattctgcat gaattgaaaa gtctgtatat
    21961 ggaaaacact gagtttgagg tacgtttctt aaaagaagaa aatagacaat tgaatcatga
    22021 tggatttgac caaatcgaat tttacattac gaccaatcca ggagagccac tgaagccttt
    22081 ggtgaaagtc gcttctggtg gcgaactgtc tcggatgttg cttgctttga aaactatttt
    22141 ttcttctgag caaggtgtga caagtattat ttttgatgaa gtggatacag gtgtgagcgg
    22201 aagagttgca caggccatcg cagataaaat ttctcaaata tcgaaatact ctcaagtttt
    22261 gtgcatcact catctgcctc aagttgctgc agtcgccgac tatcaatatt atattgtgaa
    22321 agcagtgatt gatggacgta cgcaaacttc tgtttcagag ttgaaaacaa aagaaagaga
    22381 agaagaaatt gcacgtatgc tggctggaag tgagattaca aaactcacac tcgaacatgc
    22441 aaaagaacta ttgaaactcg caaaaaggac ctgagccacc tcgcagtggt tcaggtcctt
    22501 ttcagcagct gatagctata ttttattttt acatgacact cattacagca gtcaatacaa
    22561 cagatccaat cgttaaaatc agcattgtcc aaatgacaaa ttttgttact ttagcaaaag
    22621 tacttgtttg ttttttttca cgcattgttt tcaacctcgt cattaattag ttgattcaag
    22681 tgtacaatct tttttccatt tccacaagag ggctttttta taaaaacttc ccttttttta
    22741 aataataggt agttacgata caaataaata atgttaatag aactagccat ataaaggcat
    22801 catccctttc agcaataggt aacttaatgt tcattccata gatactgccg acgattgtcg
    22861 gtatagttaa aataatcgtt aaagaagtta aaattttcat aatattattc agattattcg
    22921 atacgattgc tgaaaaagtc tgattcattt gttctactag ttttaattga atgcgagaag
    22981 tagtcattgc ctgctctgtt tccaccaata catcacgtaa atgatttttc aaagcatgat
    23041 tttcttggaa aagtttattt tgcgctagtt ctttcaatgc atctaaatta gcagaagtcg
    23101 cttctctgaa ataaacgagg cttttttgga tatccatgat ttgataaagt tgtttgtttt
    23161 cagtggatac ttgaagtttg gcttccaaag tatcgcattg agattgaagc gtagctaaat
    23221 aactgttgta agagagtgtg acctgccaaa tgaattgaaa gaagatattg agattaggcg
    23281 aaatatcact ttttggaaag gtctggctaa agactttctt taaaaacagt ggttcatgat
    23341 tcgtaattgt gataagcttc tttttgggcg tcaaaataat agagagcggg tatgtatcca
    23401 cttgaaaata accgctagga cttgttttag caaaaggata ttgtaacaat aataacgctg
    23461 gctttgtcaa ctttgattga ttgaattctt ctgtacggga attttctgca tcgtccaata
    23521 tggaagtgat gtaatctgac ggaatttcaa attgatgtgt cagctgatga atctcttctt
    23581 gattaggacg ttctacacaa taccagatcg tttggtcagt ttcctttccg gtattacgca
    23641 gtttctcatt atctaatgta aaatatttga tcatgatttt cctcctgttg cctgtttctt
    23701 ttattataac ggatagggaa ttggaataca tggaatgtgt ttgtaagata tagaatataa
    23761 ctgcataaat ttaaataaaa tgaaacagtt gtgtaaagta tatacagtat acttgaagca
    23821 acgataaaaa aggagtttac gttcatgggc acattcacac cacttatctt gttttggtat
    23881 atttacccgg taatcgtttt gtttggttgc caatttcttg taaaattatt ttcgttaaat
    23941 cgtcgtttta aaataaaagc ccctgatttg gcgattcctt ttttgtgggg cggtatgcat
    24001 gcattgtcta gaaatacagg gacaatttct atcttaccgt ttttctttat ttctgttttg
    24061 ctaatgggaa tttgtattgc tgttttccaa gcatattatt atgaagaaat tatttatccg
    24121 cgttatttca agatgacttg gcgtttggtt tttctgttga caatcgtctt gtacgctgtc
    24181 ttgatcattg tgaacatact ttcttaccta tgaaaaaaat agaaaagctg taaaaagtct
    24241 tttaaaaggc tttttgcagc ttgattttta aaaaaatgtt tatgaataaa aaaagttgtt
    24301 cccggatttc ccagaaaagg tggtagaaag tgggggattg tggtagacta attttagtta
    24361 gtggaggaat ggggggcttt caatgttgat gggtgaattc caacataata ttgatgcaaa
    24421 aggacgttta atcgtgccct caaaacttcg tgaagaactt ggcgaaaagt ttgttctgac
    24481 acgcggattg gatggctgtc ttttcggcta tccgatgagc gaatgggaaa atctggaagc
    24541 taaactgaac gaaatgccgt tggctaaaaa agacgcacgt acttttgtgc gattcttcta
    24601 ttcggctgcc actgaatgcg aacttgataa acaaggacga atcaatatac ccagcacgtt
    24661 aagaaattat gcagctttga caaaaggttg cgtagtcatc ggtgtatcta accgaattga
    24721 aatttgggat gagacacgat ggcaagattt ttctgcagca gcagaagaga actttgacga
    24781 aattgctgaa agtatgattg atttcggact ataagaatag gaagaggctg actaatgact
    24841 gaaacatttc aacactatac agtcatgttg aaagaaacgg tagatggatt gaacatcaaa
    24901 ccggatggcg tttatgtaga ctgtacactc ggcggtgccg gacatagtga atatttatta
    24961 agccaactaa gttctaaagg gcatctgtat gcgtttgacc aagatcaaaa agcaatcgat
    25021 cacgccaagc tgcgtttagc ttcttatgtc gaacaaggta tggtcacatt tatcaaagcg
    25081 aacttcagag aattagaaga acgcttgagt gagcatgtag ataaggtaga cggcattttg
    25141 tatgatctag gggtttcctc tccccagcta gatgaagcag aacgtggatt tagttatcat
    25201 caagatgcac cgttggatat gcgcatggat cagtcagctc ctttatccgc ttatcacgta
    25261 attaatgaat attcctacca tgaattagta aagattttct tccgctatgg cgaggagaaa
    25321 ttttccaaac aaattgctcg tgaaatcgaa cgagtacgaa aaacaaaacc aatccaaaca
    25381 actggtgagc ttgtcgaaat cattaaacaa gtcatcccag ctcccgcaag aagaaaaggc
    25441 gggcatcctg caaaaagaat ttttcaagct gtgcggattg cagtaaatga cgaactcggt
    25501 gccgaagagg cctctttaga acaggcaatc cggttattga aagtcaatgg aagaatcagc
    25561 gtaatcacat tccattcatt ggaagacaga attgtcaaaa gtatgtttaa agaatatagt
    25621 acggtacaag atctaccacc gggacttcct gtcatacctg acgaattcca accagaactg
    25681 aaagtcatta ctcgtaaacc aatccttcct tcagaggaag agctaacaga aaacaatcga
    25741 tcacgcagtg caaagttacg tattgcagaa aaaataagaa tgaaagagga gtagaatgat
    25801 ggcagaatta aagaaaatgc aagattacga atatgattta cctgaattgg tggatcagcc
    25861 ggaacaacaa atgcctgatt cttcttcgaa tcaagaaatc attcttccac aatcaccggc
    25921 aaaacgatta aagcagattt ctgctttgga aaaaattgct gtggcaagcg tggcgattgc
    25981 tattgttgct ttatgtgttt taacagttat gctgcgcaca aatatcagcg gagctgaaaa
    26041 agaaatcacg tcaatacaaa cagaaatcaa tgataaaaag caagaaaaaa cgagtttaga
    26101 acaagaaaaa aacgaacttt ctcgtactga acggattaaa caaatcgcag aagaaaaagg
    26161 attgtctatt aatgacgaca atttaaggaa agtgaagtaa atgagtctta aaaataaaat
    26221 tcggcggttt atggaaaaga aaaatctaaa tccgatgaac aatcgcaaaa aagtaggtat
    26281 cattttattt gctacgagta ttggattgtt ctttttattt gccgtccgtt tctcgtatat
    26341 cgttattgga ggtcatgttg ctggtacctc attagctgaa aaaacaaaac agttgtacca
    26401 aggaagtgaa gtagtaaaag caaaaagagg aacgatttat gatcgtaacg gtgtggcgct
    26461 cgcagaagat gcctcctctt attcaattaa agcgatactc tcaaaaacat atacctctgg
    26521 ggataaaaaa ctgtatgttg aagaaaagaa ttttgataaa atagcagaaa tcctgcataa
    26581 gaatctttct atagacaaaa aagatgcact gaatatttta gaggacggcg caaaaaaaga
    26641 gctgtatcaa gttgaatttg gtagctatgg gaaaaatatc tcccaagaaa ccaagcaaaa
    26701 catcgaagcg gacatgaaaa aagaaggtgt cgctggtctt tattttgtcg accatcaggc
    26761 tcggatgtat ccaaatggcg tcttttcttc tcattttatt ggatacgcag ttccagacaa
    26821 agatgagaat ggcttagttg gtaaactagg tttggagtct gcttacaatg atatcttgag
    26881 cggtaaagat ggtaagatta tttatcaaaa agataatttt caaaatcctc tgccaggtac
    26941 agtggcagaa gaagaaaaag ctgtcgacgg acaagatatc tacacgactt tagacagcag
    27001 actacaaagt tatttagaaa cactgatgga tcaagtaaat gaagaatatc agccagaaga
    27061 attgacagca gtcctgatga aagcaaaaac cggagaaatc ttggcaatgg gacaacgacc
    27121 tactttcaat cctgaaacaa tggaaggatt gactggaaaa gatgctatct ggcgtaattt
    27181 cttggtacaa gatagttatg aaccaggatc aaccatgaaa gttttcacta ctgcagctgc
    27241 tatcgaagaa ggagaattca acgagaatga aaccttccaa tctggaaaaa tccaagtaga
    27301 agatgctacg atcaatgacc atgactttgg tgaaaaaggt gtgctgacca tgcgtcaagc
    27361 tctttcatgg tcaagtaacg tcgggatggt catactggaa cagcgattag gcggacgatg
    27421 gtataactat ttacaaaaac tgggatttgg acaaagtact cattctggat tagatgatga
    27481 agtaaacggt gctttaccta cattaaatat tgtcgaccga gcaatgagtg cttatggaca
    27541 agcggtaggt gtgacgaatt tccaaatgat gaaagctttc acttctattg cgaataacgg
    27601 tacaatgatt caacctcgat atatcagcaa agtggtggat ccccaaactg gagaagagcg
    27661 aacaactcaa acagaagttc tagggcaacc tttttcaaaa gaaacaacag aaaaagtccg
    27721 tgaatatatg agagatgttg tcgaaagcga aaactacggt attgcttatg gtgtatatag
    27781 tgtacctgga tacaatgtat cggcaaaaac cggaacagcc caaatcgctt ctgatacagg
    27841 gggatatcaa acaggagata cagcctacct gtattcgatt gtggagatgg ttccttctga
    27901 agatccagac tatgtcttgt atctaacaat gaaacaccct aagacatatg atcgaatggc
    27961 attggctaaa atcgcaaatc cattgatgaa acgggcaatg gattttaaag aaactgaaga
    28021 agattcagat acagaaacaa aaacagaaaa agtatccgta gccgattatc gtaatcttga
    28081 agcggacgtt gcagcagctg atgctcaaaa aagtggactg caacctgtag tgatcggtaa
    28141 tggtaaaaaa gtacaaaaac aatctactgc caacggtgat cagttgatct ctggagaaaa
    28201 actgatccta tatactggtg gtgataagct gatgcctgac gtaacaggat ggtcaaaagc
    28261 agatatcatg aagctgggga aaatcctcgg tattgaagtt agttttgatg gagatggtta
    28321 ttgtgtcaaa caagaacttg ctccatatga aaaaatcact gaagacaaac ttagttttac
    28381 tttagaagaa taagaagaga aataggagag aaaagagcat ggaatggaca caagcattga
    28441 ttcccattgt cagcagttgt gcaatgacaa ttgccgcaat gccgctattt attggttatt
    28501 ttcaaatgaa aaagcaaggt caggcgattc gtgaggaggg accaaagtgg cacaatagca
    28561 aagccggaac tcctacaatg ggcggcttag tgtttctgat cggctctatt ttgacaggaa
    28621 tttgggttgg agcttggcaa aaacagctaa ctcctactct tttcatcttg ctttttgtat
    28681 tagcgttata cggagtaatc ggctttcttg atgattttat aaaaatattc aagaaaagaa
    28741 acatgggact gaactcgaag cagaaattga ttggacagat tctaggggga atcatctttt
    28801 atcttgttta ccgttctgaa ggttatccag gtgtattgaa tttcttcgga ctagatctac
    28861 ctttaggaat catttatggt attttcgctc tcttctggct tgttggattt tcaaatgcag
    28921 taaatctgac agatggcatc gatggattag ttgctggctt aggaagtatc tcttttgcag
    28981 cttacggtct aattgcttgg catcagcatc agtatgatgt attaatcatt tgcttaagtg
    29041 ttttaggagg tttactgggt ttcttcgtct acaacagaaa acctgccaag atttttatgg
    29101 gagatgtcgg ttcattagcg ttgggcggac ttttagcggc aatttctata atgctaaacc
    29161 aagaatggac attgcttttg attggtttga tctatgttat ggaaacagca agtgtgatgc
    29221 ttcaagttac ttcctttaag cttacaggaa aacgaatttt taaaatgtct ccaattcatc
    29281 atcactttga aatgtgcgga tggtcggagt ggaagattga tacagttttt tggttgatca
    29341 gcattgtgac ttcgttgatc acattatgga tcgtttggta ggagatgacg ggaaatgaaa
    29401 aaaatatcaa catatgaaaa taagaaagtg cttgtcttag gactcgcaaa aagcggagta
    29461 agtgcggcca aattattaca tgaattgggc gcacttgtca ccgttaatga tggaaaacct
    29521 tttgacgaaa atccggaagc acaagagctg ctttctttag ggataaaagt aataacaggt
    29581 agtcatccaa tcgaattgtt ggatgaagaa ttttcattga tggtcaaaaa tccagggatt
    29641 ccctacagtc atcctttagt agcaaaagcc caagaaatgg gtattccggt aattacggaa
    29701 gttgagctag cttacgaagt agcggaatgc ccaattattg ggattacagg aacgaacgga
    29761 aaaacaacga caacaacgat gacaggacta ctactgaatg caggagcaga tcaaggaatt
    29821 gcacgtttag cgggtaatat tgggtatcca gcaagtggtg tagcgcaaga agcaaaatca
    29881 gaagataaaa ttgtgatgga attgtctagc ttccaactca tgggaatcac agatttccgc
    29941 cctcatatag cggtgattac caatatctat gaagcacata ttgattatca tggtacaaga
    30001 aaagaatatg tcaaagctaa atggaacctt caaaaaaaca tgacagaaaa agattatctg
    30061 atccttaact ggaatcagtc agaactacaa gaattagcgc aaagaacaaa agcacgcgta
    30121 ctgcctttct cgacaaaaga agtgttggaa gacggtgtgt atgctgacga ttactcaata
    30181 tactataaaa aagaaaaaat aatggaaata tctgaacttg gcgtaccagg taagcataat
    30241 gtagaaaatg cactagctgc tatttctgta gcaaaattgt atggtatatc aaatgaagct
    30301 atacgagaaa cgttgcattt tttccatggc gtaccacatc gtacgcaata tgtaggagaa
    30361 atccaaggca gaaaatttta taatgattca aaagcaacca atattttagc gactaaaatg
    30421 gctctaagtg gatttgaaac gtctaaggtc gtgttattgg ctggcggatt agacagagga
    30481 aatacatttg atgaattaat tccttcatta aaaggaatca aagcaatggt cgtatttggc
    30541 caaacaaaag aaaagttgat ggatgctgga aaaaaagcag gcatcgagac aatagtaaca
    30601 gccgattctg tagaacaagc agttccttta gcattagaaa acagtacaga tggtgatgta
    30661 gttttattat caccagcgaa tgccagctgg gatcaatacc caaattttga aactagagga
    30721 aacagattca tggaagctgt gaaccgatta aaatagaaaa agtagaagcg agcgataaaa
    30781 aatgaaaatt ttggttacag gcggaggaac agggggacat atttatccag ctcttgcctt
    30841 tgtcaattat gtaaagactc aagaaccgaa tgctgaattc atgtatgtgg gagcaaaacg
    30901 aggtctagaa aacaaaatcg tccctgatac aggaatgcct ttccatacct tggagattca
    30961 aggatttaaa cgaaaaattt ccatgcataa catcaagacg gtccgattgt ttcttaagag
    31021 tatccgtcaa gcaaaaaaaa tcctcagtga ttttcaacca gatatcgtta tcggtactgg
    31081 aggatatgtt tccggagcgg tagtgtacgc tgcagccaaa atgggtattc caacagttat
    31141 ccatgaacaa aacagtgtac caggtgttac caataaattt ttaagccgat atgtggatcg
    31201 gatcgctctt tcatttaaag atgcggcttc cttttttcct gaaaacaaag cagttcttgt
    31261 aggtaatcct cgagcacaag aagtagcaga tactaaaaaa tcagatattt tgaaaacatt
    31321 tgatttggat ccagaaaaga aaacagtctt gatttttggc ggtagtcaag gagcacttaa
    31381 aatcaatcaa gcagtatcag tttttttaag tgaatatatt ccttcttctt accaagtttt
    31441 gtatgcttca ggagatagat attatgaaga gatcctaaat aaaataaatc ctgaaataca
    31501 aaatattagt gtccagcctt atatcaaaaa tatggcagag gtcatggctt ccagtgatct
    31561 gctggtgggg cgtgctggtg caacttcgat cgcagaattc acagctctgg ggttaccggc
    31621 agtgttggtt cccagcccat atgttacgaa tgatcatcag actaaaaatg caatgagtct
    31681 tgttcacgca ggagcagcaa aaatgattgc agataatgaa ctaacaggtg aatcactaag
    31741 tcagacagtc aacgaaatca tgggtgatga agaattgcaa aagcaaatgt gccgagcttc
    31801 taaagaacaa ggaatccctg atgcttcaaa acgtctttat gacttagtaa aacaaataat
    31861 caaaaaataa aggcggtggt cttagtgagt aagaaaaaag aaccaaaaaa attgtctcat
    31921 ccgtcttctg atgaaaacaa tttgactcct tggcaaaagg ctaaccgaaa atatctggca
    31981 gaacaagaga tgaaaaacca agagccaaaa aaagaagaca taacattaga tacacccctt
    32041 tcagattctg aagaagaaaa aacggttcaa gaggaacagc aagaagaaag ccagtccgag
    32101 aaaaaaatcg aattttttga agagatcaag caaaagaaaa acggtggacc atataacggt
    32161 tcttttttga accggctgcc caatttaaaa agtcaacggc acaaagtact atttcgtcgg
    32221 ttagctttga tcatcactgt attgggtata ccgctgattt ttttgatcta ttatgtttct
    32281 ccttatagca aattacaagc agttgcagta tcaggcaata agacagtcaa cagccaagaa
    32341 atcatctctg atacaaaatt gtcattagga gaaaatgttt ggggacagta ttttcatcga
    32401 tctacttata tcgatcgcct taaaaaagca cagccaagaa tcgaaacagc aaatatacat
    32461 ttcaaaggga tgaatgaatt cgatttggat gtaacagaat ataaagagat tgctttgatc
    32521 gcaaaaaaca atcaatacta cccagtgatt gaaaatggaa cagtattaga tgaaaaagta
    32581 gcaaatccta caaaaaatct ccctatcttg gaagagttca aagactctgc aaaaatcaaa
    32641 gaattaacaa agcaatacaa tcagttatca tcagaattac aaaaagcaat ttctgaaatc
    32701 aagtacacgc caagagcaag caataaaaat ctgatccaat tgaatatgaa tgacggcaat
    32761 caagttattg taaatatcaa taatcttgcg aaccaaatga aatattattc gcaagtagcc
    32821 aaagatatgg atgaaaaagg tgtgatcgac atggaagttg gtatcttctc gcatccttac
    32881 ggtagcactc aagaaacgga agaatcgggc caatcggaag aaacaactga aagtgaagaa
    32941 aatgcggatc aaacaaacga atcaagtgaa gaggcagaaa cgcaaaaagc acaaacacct
    33001 ggatcgtcta gcacaaacgg atcatctgat tcaaatgatt caagtgaaac aagtcaaata
    33061 gaagataatc caagtacaac tccgtcaagt ttaaatgaaa attgataaaa caactttaaa
    33121 aagtaagaat gtactttcta aaaaccagta atttatggta gaattggaag cagtgaatat
    33181 atctattaat aagtatattt taaaacggac gctagttgtt cgcttgtata tggaatagta
    33241 agtaggaggg aatcccattc atggcaaaaa caggaatgta tgtaggcctt gatattggaa
    33301 cgacatctgt taaagttgtc gtggctgaat atatcgacag ccaaatgaat attattggag
    33361 taggaaacgc aaaatcagaa gggattaacc gaggcattat cgttgatatc gataaaacgg
    33421 tccaagcgat acaacgagca gtacgacaag cagaagaaaa agcaggtatc cagatcaaag
    33481 gcgtaagtgt cggtttgcca gcaaacatgc ttgaagtaga aaattgccag ggaatgattg
    33541 cagttaacgg agattcaaag gaaatcaccg atgaagatgt cagaaatgtg gcttcagctg
    33601 cccttgttcg ttcaatacct cctgaacgtc aaatcgtgtc aatattgcca caagatttta
    33661 ctgtcgatgg gttcgaggga atcaaagatc ctcgtggaat gatcggcgta agattagaaa
    33721 tgtacggtct gttgtttaca ggacctaaaa cgatcatcca taacatacgt aaatgcgtgg
    33781 aaaatgccgg attaatcgtc aacgaaatgg tgattactcc tttagcgctg actgaatcaa
    33841 ttctttctga cggagaaaaa gattttggta cgatcgtcat tgatatgggt ggcggccaaa
    33901 caacaacggc tgttatgcat gataagcaat tgaagtttac caatcttgac caagaaggtg
    33961 gcgaatttgt cactaaagat atctctattg tattaaatac gtcatttaac aatgcagaag
    34021 cgttgaaaat aaactacggg gatgcatacc cagaacggac atcaccagat gaagaatttc
    34081 cagtagatgt gattggtcag tcagaaccag tgaaagtcga tgaacgctac ttgtcagaaa
    34141 ttatttcagc tcgaatggaa cagattttca acaaagcaaa agatgcattg gaccaaatcg
    34201 aagcattaga gttgccaggt ggcgtcgtat tgacaggcgg agctgctagt cttcctggtg
    34261 ttgtggactt ggcacaagaa atcttcggcg taaatgtaaa attgtatgtt ccaaatcaca
    34321 tggggctgag aaacccggta tttacaaatg taataagtat cgtggattat tcagcaaact
    34381 tgagtgaagt gtatcaattg gcaaaaattg ctgttacggg tgaaacctca gcagctcgtc
    34441 aaatggtagt tgaacaagaa acaacaaaca catatgagag ctatgaagca cctgaagaaa
    34501 caatctacga tgagccggaa ccaaaagagt ccggagaaaa tgtaaaaaat aaaatcaagg
    34561 gtttctttac aaatattttt gattaattca tcctgggagg aataagatat ggaattctca
    34621 attgataata atatcaatga cggcgcagta atcaaagtca tcggagtcgg tggtggcggt
    34681 ggaaatgctg taaaccgaat gattgaagaa aacgttaaag gtgttgaatt cattacagcc
    34741 aacacagacg tgcaagcatt aaaaaattca aaagctgaaa cagttatcca attaggcccc
    34801 aaatatactc gtggtttagg tgctggttct cagccagaag ttggacaaaa agcggcagaa
    34861 gaaagtgaac aatccttacg cgaagcttta gacggtgcag atatgatttt catcactgca
    34921 ggaatgggtg gaggaaccgg tacaggtgct gcaccaatcg ttgcaggtat cgcgaaagaa
    34981 ttgggcgcat tgactgttgg tgtcgtgaca cgtccattca cttttgaagg accgaaacgt
    35041 ggccgttttg ctgcagaagg tatcgctcgt ttgaaagaaa atgtggatac attattaatt
    35101 atctcaaata accgtttatt agaagttgta gataaaaaga caccgatgct tgaagctttc
    35161 cgtgaagcag ataatgtttt acgacaaggt gtacaaggta tctctgattt gatcacagcg
    35221 ccaggttacg ttaaccttga ctttgctgac gtgaaaacag tgatggaaaa ccaaggtaca
    35281 gcattgatgg ggatcggtgt ggctagtgga gaagatcgcg tagtagaagc aacgaaaaaa
    35341 gcaatctcct ctccattgct tgaaacatct attgatggag ctgaacaagt acttcttaac
    35401 atcacaggcg gtttggatat gacattgttc gaagcacaag atgcttcaga tatcgtagca
    35461 aatgcagcaa ctggcgatgt aaatatcatt ctaggtacat ctatcaatga agaaatgggc
    35521 gatgaaatcc gtgtgacggt catcgcaaca ggtatcgatg aatcaaaaaa ggaacgcaag
    35581 tctagccgac cagctagaca agcacaaatg caatcacctg ctcaaaagac tgtattagat
    35641 atggatcaag ctaaaccaac ttcttcagaa gaagaaaaca gttttggcga ttgggatatc
    35701 agacgtgaac aaaatgttcg tcctagagtt gatgattcca attttgacaa tatcgaaaaa
    35761 aaagaatttg atacattcaa ccgcgaagaa acaaaatctc atggcgatga tgaattaaat
    35821 acgccaccgt ttttccgtcg taaaaaataa aggagaggaa gagcacatga ttgctgacaa
    35881 cttgcagaaa atcaagcaag aaattcagga ttcgtgtgct cttgttcatc gtgatccgaa
    35941 agaagttaca ttagtagctg taacaaaatc tgttgaatcc gctcaagctg aagaattggt
    36001 tagagctggc gttatgaatt gtgcagaaaa ccgagtagat aaattattag aaaagaaaca
    36061 agatttattt gattttcctg aaataaagtg gcatttaatt ggtaatttac agcgcagaaa
    36121 ggtaaaattg attgtaaatg aaattgatta ctttcatgcg ttggacagtt taagattagc
    36181 agaagaaatc cagaagcggt tagagaaaga attatcctgt tttattgaag taaatgtttc
    36241 tggtgaggaa tcaaaacatg gaatttcacc agatgagctt ttaccgtttg tggaatcttt
    36301 agctgcctgc acaaaaatca aggttgttgg gttgatgact atggcgccta aagaagcttc
    36361 tgatgagcaa atccataaag tgtttggtac gctgaaagaa ttgcgtgatc ttgttcaagc
    36421 aaaaggttat gcccatgcac catgtagtga attaagtatg gggatgagtc aagactttca
    36481 aattgcgatt gaagaaggtg caacatttat ccgtgtagga acagcgctat tcaaagacga
    36541 ataaaggagg aacaaaaatg ccgcttttaa acagaggagc aattgctaat tttttcggat
    36601 tagctgatga ggaagagtac gaataccaag attatcctga aacaacaaaa aaggctgtac
    36661 agccaactaa aaaaaataca acacagaatc aatcaactca ttctgtatct gctgcgaata
    36721 ctcatgaaaa acaaaaaacg gcgacttcac gtcctgtaac gcatgaaagt attcagccgg
    36781 ctgaaaagaa aaaagtaatt gaactacatc aatcacctac aaaaactgta gcgaaagaaa
    36841 gaacagcttc aagaacctct acacaaccta aagcaaatct tgctggaaaa atcactgtaa
    36901 tggaaccaag agcttattct gaagctatgg cgattgctaa acgaatcatt gctggagaag
    36961 caaccttagt gaattttcat ttagtagaag aaaaacaagc gagaagaatc gttgattttc
    37021 taacaggaac agtttatgca ctagatggag atatcaaacg tgttggagat gagatttttc
    37081 tttgtacacc gactgggatg gagattgaca gttcaaccgc tcaatctttt gcagatgcga
    37141 atttgtttga tctgtaagag gaggaactac gatttatact attgcactat tacttcattt
    37201 acttcaacgg gcagcctatt tctatagcat tatcttgatg gtatatgcat tgctctcttg
    37261 gtttccaggc gggtatcaat cagggctagg acgattatta gctaagatct gcgaaccgta
    37321 tatcagtttg ttcgatcgtc tgcctttgag gattggaccc ttggatttca caattttagg
    37381 ggcaatcctg atcttgaact tagccgtaca aggtttagca cagatcgtta tatggataac
    37441 tgtccattta gtttattgat tgaagcaaca aaatctttat tctcagaata gataggaggg
    37501 gaaaattttg gatgcaaatg tataccagca ctttcgggca gatgaacgta catttatcga
    37561 ttcggtaggc gattggattg aacaggttca aggacaatat gcaccctatc tgaccgaatt
    37621 tcttgaccca agacaatcct atattttaga aacactgatt cgacaaagca gtgatttgcg
    37681 tttcatgttt tatggcggat atgaacaggc agaaagaaaa agatgtttga ttttccccga
    37741 ttattacgaa cctgtagagg aagattttga aacagcattg tatgaaatcc attatcctag
    37801 caaatttgcc agcctaagtc atgggaaaat ccttggaact ttagtgggaa cggggatcaa
    37861 acgctcttac tttggcgata tcatctcgga cggtgatcgc tggcaagtat ttattgcaaa
    37921 tgaggtggat cattttgttg ctgcacaagt aacaaagatt ggaaaggttg ctgtacgctt
    37981 ggaaccaatc aaatacacag aaatgattat gccgaaagat agctggacac aagaaagagg
    38041 aacagttagt tccatgcgac tagacagtgt gatctctgag atttataata tttccagaca
    38101 gcggtccaag cagctgatcg aagctggtaa agtaaaagtg aactggacag aaaatacaag
    38161 acctgatttt gtacttgaac tattagatat tatttcagtc agaggttatg gcagagtcca
    38221 gatccaagaa cttgagggga agacgaaaaa agaaaagtac agggtactgt tcggtgtatt
    38281 acgtaaataa aataaaaagg tggtttactc atggctttaa ctccattaga tatccaaaat
    38341 aagacattcc cgacaaaaat gcggggatat aatcaagacg aagtagatga tttcttagat
    38401 ttagttgttc gtgactatga ggaattgact caaagaaatc gagaattaga aaaagcagtc
    38461 aaacattctg aagaaaaact tgagtacttc aatgaattaa aagatgcttt gaaccaatca
    38521 atcatcgtag ctcaagatac ggctgataaa gtaaaaacaa gtgcaagcaa ggaatccgaa
    38581 gtgatcgtta cttctgcaca aaacaaagca gatgagttag ttgcgaacgc tgaaaaacgt
    38641 gcacatcaat taacaactga tgcagaagaa aaagcaagaa aaatcttgac agatgcaaca
    38701 gaaaaagcaa gacagcttgc gacagaaact gaagatctta agaaaaaaac acgagtattc
    38761 catcaacgta tcagcttgat gcttgagtct caattagaac aagtgaaaag tccggaatgg
    38821 gatgaaattt tacaaccatt ctccagctac gtgactgaca gccatgaagt gatcaaagaa
    38881 gttttagcaa aacagcttga caatgaaaat gatactgatg taaactcaga cacaggagtc
    38941 atagaagcac ctatgacggc atttgataca caggctattg atatctcgat cgtgcctggt
    39001 gaaaatgaaa acgagtagaa cagaaaagca ctttgttatt cttaaagcga gccgatgaga
    39061 gtggaagttc ggtaagacac tgcatggtga aagatcctct acttgagacc tgcttttccg
    39121 aaattagagt aagaagcagc ggctgacctc gttattggtc acagagagtt tgcactagat
    39181 agatgcaact aaatttgggt ggtaccacgg catgttcgtc ccttgcggct gaacatgctt
    39241 ttttgtgtcc ggaaaacaaa taaaggagtg tatggttgaa tgaaaatgaa agaaaccttg
    39301 caattaggaa aaacaaaatt ccctatgcgt ggtaatttgc caaaccgaga aggcgagtgg
    39361 caaaaagaat gggaagaaaa tcgaatctac caaaaaagac aagagttgaa cgaaggaaaa
    39421 cctagctttg ttcttcatga tggacctcca tatgccaatg gtaatatcca tttaggacat
    39481 tcattgaata aaatcagtaa agacattatc attcgttcaa aatcaatgtc tggctatcgt
    39541 gcaccatatg ttccaggatg ggatacacat ggattgccga ttgaacaagt attggctaat
    39601 aaaggcgtga aacgtaaaga aatgtccatg gcagaatact tggaaaaatg tcgtgaatat
    39661 gcactttccc aagtagataa gcaacgagca gatttcaaac gcctaggagt tgcaggagat
    39721 tgggaacatc cttatgttac attagatcct tcttatgaag ctgcacaagt acgcgtattt
    39781 ggtaaaatgg ctgaaaaaag ctatatttac aaaggattga aaccaattta ctggtctccg
    39841 tcaagtgagt cttctttagc ggaagctgaa attgaatata aagatgtaaa atctccatct
    39901 atttttgtag ctttcaatgt caaagacggc aaagatattc ttgatgaaga tacagctttt
    39961 gtcatttgga cgacaacacc atggacattg ccagcaaacc aaggtattgc tgtaaatcca
    40021 aaatttacgt atgtattggt ggaagccgat ggccgtaagt ttgtcgtagc gaaagatttg
    40081 ctagaaacag tccaatcaga aatcggttgg gaaaatgtaa cagttctaaa agaatttgct
    40141 ggacaagaaa tggaatatat gactgcaagt catccattct atgaacgtga atcacttgtg
    40201 attttaggcg atcatgttac tttagacgct ggtactggat tagtccatac tgctccagga
    40261 catggggaag atgactatat cgctggtagc aaatataaac tatctgtagt gtcacctgta
    40321 gactcaaaag gattgtttac agaagaagca cctggatttg aaggtatctt ttatgacaaa
    40381 gcaaatccta tgatcactga tctattaaaa gaaaaaggag ctttgctgaa gctagatttc
    40441 tttactcata gttatcctca cgattggcgg acgaaaaagc ctgtgatcta tcgggcaact
    40501 ccacaatggt ttgcatctat cgataaattc cgtcagaata ttttggatga agtagaaaaa
    40561 gtagagtggg ttatcccatg gggtaaaaca cgtttgtata atatgatccg tgaccgcggc
    40621 gattgggtaa tttcccgtca gcgtgcatgg ggagtaccac ttccgatttt ctatgctgaa
    40681 aatggagaag caatcatcac accagaaaca atcgaacatg tggcaaaatt atttgctgaa
    40741 catggatcaa ttatctggtt tgaaaaagaa gcgaaagaac ttcttcctga aggatttacc
    40801 cacccaggtt ctcctaatgg cgaatttaca aaagaaaaag atatcatgga tgtctggttt
    40861 gattctggtt cttctcatga agctgtttta cgtcagcgac cagaattatc attcccagca
    40921 gatatgtatt tagaaggatc agaccaatat cgtggctggt tcaactcaag tatcacgact
    40981 agtgtggcta tcaatggtgt agcaccatac aaatcagtta tttctcaagg atttgcttta
    41041 gatggtgaag gccgtaagat gagtaaatct ttaggaaaca tcattgcacc agaaaaagtg
    41101 atcaaacaat ttggtgcaga tatccttcgc ttatgggtat ctagtgtaga ttatgaagca
    41161 gatgttcgtg tttctatgga tatcttaagt caagtggcag aagtatatcg taaaatcaga
    41221 aatacgatgc gtttcttatt agcgaataca gaagatttcg atcctaaaaa agatactgtt
    41281 tcgtacaatg atttacgttc tgtagacaaa tatatgaccg ttcgattgaa ccaagtaatc
    41341 aaagaaatcc gagaagaagg gtacgacaaa tataacttca tgcatattta ccgtacagtt
    41401 atgaacttct tgacggttga tctatcgtca ttctacctgg attttgctaa agatgtagtt
    41461 tatattgaag cagaagatga ttatcaacgt cgttgcatgc aaactgtttt ctatcaaaca
    41521 gctgtagctt tgacaaaatt attgactcct atcattccgc atacagcaga agaaatctgg
    41581 tcattcctga aagaagaaga ggagtacgtt cagttaagcg agtttcctgg ttatgaagag
    41641 tttgcaaacc aagaagaatt gatggatacc tggactgctt tcatggactt ccgtgataat
    41701 gtcttaaaag cattggaaga agcaagaaac agcaaattga ttggtaaatc tttggaagca
    41761 aaactaactg tttatccaaa acaacaagtt cgtgaaatgt taaaagcttt ggatgcagat
    41821 attgcacaat tattgatcgt ttcaccagac tactttgaag ttatggcagc agatgaacaa
    41881 gtgccagata acgctatggt atttgatgat gttgctatta cagtggaaaa agcagacggg
    41941 gaaacttgcg atcgctgtcg ccaagttcgt aaagatgtag gagtagatga aaaacttcct
    42001 catctatgcg gtcgttgtgc aaaaatcgtt gaagacttct atcctgaagc tgtagctgaa
    42061 ggattcgaag aaaagtaaaa gggaaaaggg ggtgtgacat gttctttgtc acaccccctt
    42121 ttgttatcaa tcgtttaatt ttccacgttc acgataccag ttatctggtt gccagatcca
    42181 ttcgaatccg tctttttcaa gtagttcgaa agctgcttta ggtcccattg atccagcttt
    42241 gtagttggga aaatccggtt gtgtttggtc ccaagctttt cgaatgacat ccacgatatg
    42301 ccaagattga gcgacttctt cccaatgaga gaagttcgtt ccatcaccat tcaacgcatc
    42361 taatagtaat ttttcgtaag cttctgggct attttcaacc atttctgcac tatttctaaa
    42421 ttccaatttg attggttccg tttcaaatcc ttgccctact tctttcccat tcaaggataa
    42481 agaaaatcct tcagttggct gaatatagat cgttagaaca ttaggtggaa gtgtcgtatc
    42541 gtcacatggt tcatcgacag atgttttgaa tacattaaca ggaacttgtt tgaatacgat
    42601 gttgatccga gtcccttttt cagtcaaacg tttgcctgta cgtacataga aaggaacacc
    42661 tgaccaacgg aagttatcga tagtgaattt gcctgcgacg aatgtttcag tctctgattg
    42721 agcatccact ttgtcttctt cgcgatagcc cttgaattgt acaccatcca attcaccttg
    42781 agcatattgc ccgcgaacga agttttctaa cacttcttct tcgttataaa tgcggatagc
    42841 tttcaatgct ttgatctttt ctgtacggat ttctttttcg gaaaatgcta ctggcggttc
    42901 cattgcaagt aatgctacaa cctgtaaaat atgattttgc accatgtctt taagtgcgcc
    42961 actatgatcg tagtagcccc cgcgatcttc tacaccgagg ttttcgccaa aagtaatttg
    43021 gatattgtcg atatagcgat tgttccattg agcctcaaaa atattattgg caaaacggat
    43081 agctgagatg ttttggatca tttcttttcc taaataatga tcaatcctga aaatatcttg
    43141 ttctgggaat acttctctga tttgctggtt caattcaaaa gcagactggt aatcagaacc
    43201 gaacggtttt tctatgatca aacgattgaa gccatttttt gtcataatat tttgtgattt
    43261 caaatgagaa acgatcgttc caaaaaattg aggtgccata gccaaataat agacacggtt
    43321 accgtttaat tgatagtgtc catctaatcg gtcagacaat tcttttaatg tctgataatg
    43381 cgttgaatca tttacatcat gggattgata gtaaaaatgg cttgcaaagg tagttgcttc
    43441 ttcatctgaa ggattcaatg cacggattgt ttctttgaca acttcacggt agtgctcatc
    43501 gctccaggga cggcgagcgg ttccgattac agcaaaatgc tcgcctaaat ttccttttct
    43561 gtaaaggcgg aacaaagacg ggtacaattt acgctgggct aggtcacctg taccgccgaa
    43621 tatcgtaaac aatacatttt tttcattaga catgtggata cactccttta tattttatcg
    43681 acaaaaatcg tgctggccgc cttaaagctg acggggattt tctttctatc attttggata
    43741 gtgatcggtc cttcataagc tgcgatgtca acgatctcat attcttcgtt gatattcagg
    43801 tcaatagtga ctaagtaatc caacaattct ttttcatcaa gcactcgtgc gatacgcact
    43861 tttgtaccga caggatagga ctccaatgtt tgtcggcgtt cttcatggac aacttcctct
    43921 tttttaggaa tcgatcctcc atgaggacaa tgttcaggat gattcaaata gttttctaaa
    43981 tgatctgcaa gatgttccga agtcacatgt tccaatactt cagcatcatc atggacttca
    44041 ttccagctat agtctaaatg ttcaactaaa aaaacttccc ataagcgatg tttgcgaatc
    44101 agactgctag ctttttgcag tccgcttgca gtcaattgta caccttgata aggggaatgt
    44161 tctacaagct cttctttcac aagtttagaa atcatttcac tgacagatgc agctgagaca
    44221 tctagactag atacgatttg tttattgttg actttcgttg tatctccacc tagttctaaa
    44281 atgattttca aataatcttc tcgatttggt gtcatattcg atcatccttt catggtaaaa
    44341 aaccatcatt agtaagtgta acaaaaaaaa cgttaattga ctatttttct aaataaaaaa
    44401 caaaggattt ttagacgttt ttacaacaaa aaacgaaaga tgatagagaa gactcattct
    44461 ttcgtttata aaggatatat atatacattg ggagggatct atttttcaga taacaactta
    44521 tatgaagtgt ctgcagattc gtcataaaga gtttgttcgg gataagaaaa gataagataa
    44581 aaaccatact gatcacgtac gactaaacga tattcatcta tttctaccac agatgtgcca
    44641 agagactgac cgttgatcaa aaacgtgtaa tcagatgtaa tctgtaattg ttgtttttgc
    44701 tggctatcac taaagaacca tgtaccttga tactttgaga gtccttctga cttttccgct
    44761 tgttttttct ttttcaagta aataaaactt gagagaacgg taatgagtga accaagaaaa
    44821 tagatcattt tttttatcac caaatcactc ctttatgaga aaataatcgc atagacatat
    44881 tctttcttct ttatttcatt gtaccgaata aaagctaaaa tttctctaag actttcgact
    44941 ggttttttta ctgtttttct acaaattatg ataggataca tagtaggaag aaaggtgaca
    45001 taatttgaag tatcagcaag tggaaatagc tttttttatt gaccgcccga atcggtttat
    45061 agcacattgt atgaaaaaag acggagaaat aattattgcg catgtcaaaa atacaggtag
    45121 aggaaaagag gtcctgatac caggcgctga agtggctatt gtatttgctc ctggacctaa
    45181 acggaaaacc gcctatgatc tgattgctgt aaaaaagaag ggtgactggt ttaatatcga
    45241 tagtcaattg cctaatcggc tggcaattga tggtatttta gacggcacga tccttttacc
    45301 aggattaact ggtccattta cggtatacaa gcgtgaagta acttatcttc actccaaatt
    45361 tgatatttat ttagagacgg tggtcggaca aaaagcattt gttgaagtca aaggtatgac
    45421 acttgaaaat aaagcgattg gtgcttttcc tgatgcgccc acactcagag gattgaaaca
    45481 tataggagaa ttaacgtatg ccgcacaaga tggttatgcc gcttatgtct tgtttattgc
    45541 tcagttcgaa catcttcatc tggcaacgat ccatgaagaa atgcaaccag cactagctga
    45601 tatggttcga catgcgcagc aaagcggagt tcaaatcctc gcctataatt gtcaagtatc
    45661 ggcagatgag gtaactgtca agcaggccat tccatttgat gtagatatcc cttttgaaga
    45721 tcctatacaa tagaaagaag gtaaaataat gatcaactta ggtattatcg gaacaaattg
    45781 gattactcat caatttgtcc aagcagcttt aagcacaaaa aaatatgaat tgacagcggt
    45841 atattctaga catttggaaa aagcacagga atttggttct cattatgaag gggatatcga
    45901 attcgcaaca gatttgaaga cttttttcaa tattactcat atggacacca tttatattgc
    45961 ttctccgaat agtcttcact ttagtcaagc taagcaagca attttggcag gaaaaaatgt
    46021 gatagttgaa aagccagctt tttcgacgcc aaaagaaatg gatgaaatca tccatttggc
    46081 aaatgaacac aatgtgtatt tttttgaagc agcaagaaat atccatgaat caggttttaa
    46141 aaagattgcc gattacttac cattaaaaga cgagataatc ggtgcaaatt tcagctatat
    46201 gaaatattct tcgcgctatg accaagtatt agatggagaa gaaccgaata tcttctcgac
    46261 tcatttctca ggtggtgcat tgatggattt aggtgtttac cttgtatatg cagctgttgc
    46321 ttggtttgga atgccaaaag aagtccatta tttcccaaga aagatttcta ctggtgtaga
    46381 tggtcttgga tgggggatct tacgttatga gaattttgat gtatcgatcc agccaggtaa
    46441 aatcggtgat tcttatttac cgtcagagat ttattttgac tcaggaacgc tagtgatgaa
    46501 tggagtcaat gcaattgaac aagcacaatt tcatgatcgc gcgcataaag aaatcattga
    46561 tttggcggta acagctgcag aaaatccaat ggcagatgaa gcagaggatt ttgccaatgt
    46621 catggctcat ccgaaagatc cagcttgggg attgttgtat gaagagtgga tcgagctagc
    46681 tagaaatgtg aaccaagtga tttatgattt aagaaaaaat ggcggcatca aattcgatgc
    46741 agataatgag gaagattttt aatgaacgaa tttgtacaag tatttcccga aaattggcag
    46801 aaaaaatatg aggataaagg gtttatctca ccttctctta ttcaacgagc agtttttcag
    46861 ccactaagta ataaacaaag tatagtagcg gtttctccta caggttcagg taaaacactg
    46921 gcttatcttt ggcctctact actaaacgtg gaaccaggtg aagcaagtgc gttggtcatt
    46981 tttgcttctt ctcaagaact agctattcaa gtagcagatg ttgcacgtga atggggaaaa
    47041 gacaaagagt tgaaagtcca atctttggtg ggcggagcca acgtcaaacg tcagattgag
    47101 ggtttgaagg aaaaaccaga aatcttgatt ggtacaccag gaagaatatt ggaattgatg
    47161 aaagcaaaaa aaataaaagc acatcaagtg aaaacaatgg tatttgatga agcagaccag
    47221 ctatttgacg ctgggaacag ccaaatcatt gatcaaattc ttcatcaagc tccaacagag
    47281 tatcagctag cttttttttc agcgacagca gatcgttccc ttgaaagcat tgaaaaaatc
    47341 actgggaaaa cgtttgagac aattgacgtg acagcagagg acgattctcg aaaaggactg
    47401 agacattact ttttacgtgt accgatacga aagaaagatg actacttgag acgtctcacg
    47461 catatagaag attttcatgg tctgattttc tttaatcaac tgactgaatt aggcgtaatg
    47521 gaagaaaaat tgctgtatag aggtgtaccg gtaggaagtc tggcctctga tcagaacaag
    47581 ctactccgaa aagcagcgct agaacaattc aaaaagaaaa tggtcagagc acttttgacc
    47641 acagatgtgg cggctagagg tttagatatt acaggtcttc cttatgtagt gaatgcagaa
    47701 gttccgttaa gtgaagaagc ttatttgcat cgttcgggaa gaactggtcg aatgggtaat
    47761 gaaggaatag taattacact cgtacaagat aataatctaa gagatttaaa acggctttta
    47821 cgtccatcaa atataacact tgaagagatt tttttatacg gaggtaagct gcaaactgag
    47881 cagccaacca aagaagatgc agctcctgca gtaaataaaa agtatgcgaa aactttttct
    47941 ggaaaggagc agaacccaga atataacaaa gaaaaaataa gaaaaaaagt taaaaataaa
    48001 tcaaaaaaag agagaaataa aggagcaagg cgcaaataag cgccttttta tgctatgatt
    48061 atgaaaacgg gaacaaaatt ggttgccaga ggaggggtta tgtgaaagaa cgtcaagaag
    48121 agatcttgca gctattgaca gaaaaccaac aaggattgac agcaagtgat gtagctgagc
    48181 gcttaactat cgatcgaagc aatgcaagcc gatatttaag tgaactatac aaggcccatc
    48241 atattgtaaa aaccgctggc cgaccagtcg tttactcttt gccaacggaa aagtcaaaat
    48301 ctgatgaagt gcatgtcgac tcttctacgc aagtgacttt tgaaacactc gttggagaaa
    48361 atgattcttt aaaagtcagc atacagcagg caaaagctgc gattctttat ccaccgcggg
    48421 ggttgcatac gattattttt ggagaaactg gtacagggaa gtcgatgttt gctgaatgta
    48481 tgtatcattt tgcaatcgat tcagagatgc tttctgcaga tgcgccattt gtttctttca
    48541 attgtgcgga ttatgctcaa aatccgcaac tgctctttgg acacatcttt gggatcaaaa
    48601 aaggggctta cacaggagca gctcaagata gtcccggtct aatagccaaa gcagacggcg
    48661 gtatattgtt tttagatgag atccaccgtt taccgccgga agggcaggaa atgctgttta
    48721 ctttcattga taaaggtatt taccgtccgc ttggagaaag tgcacaagta catgaagcat
    48781 ctgttcagat cattggggcg acgactgaat cttcagaaag tttcttatct acgttcaatc
    48841 gacggattcc tatggcaatc accttgccga atcttgcttc acgttcactg gatgagcgtt
    48901 atgaaatcat ctctcttttt atcaaacaag aagcgaatcg tttgaatcaa cgtatcgata
    48961 tcgaaaaaga agcgattctt gccttcatgc tttatgatgc ggaaggaaat atcggtcaag
    49021 tgaaacgaga cttgaaacta gtctgtgcaa aatctttttt acattaccgt actcatcatg
    49081 agaaaaagct aatcattcga aaagaagaac tttcccttca agtacaaaaa ggattactga
    49141 aaatcaagga aataccggaa cgtctcgacc gattcatgga cagcaaaagt cagtatttga
    49201 catttgaacc aggatttgct gatgttgtct ggtcacaaga tccagaacga aacatgcagg
    49261 tctataacga tattgaggaa aaagtcactt ctctttccga aaccggtgtg gagaacattg
    49321 atctagagac cctcatttca aaagatgtgg atgcttattt ccagacatat gtcaaagaat
    49381 tgactaaaag ttcgattccc aaagatcttt tgccagatga aatctggcgg ttaaccaatc
    49441 aattgtatga tgtggctgaa aaacggttag accgtattta taatgaaaaa gctcgttttg
    49501 cttttgcttt gcatctgcaa agtacattgg atcgtgtgag agaaggtcat atgatcgtcc
    49561 acccagattt gaataacgta cggaaaaatt ttaagaggga attccaggta gcaatcgatc
    49621 tttcaacgat cattgaagaa gaagaacaag ttgagattcc ttttgatgaa atcggcttta
    49681 ttagtatgtt tctttctatc aatctaggag aaagtgagcc tgttaaagaa aacaaagtag
    49741 atgttctcat attgatgcac ggcagacaga ctgcttcaag catgttggag atggcacaag
    49801 aactattagg aacaaaagta gggaaagctt ttaatatgcc actggttatg gaagttcaaa
    49861 caatgtatga agaagtgtta caatatgtca aaaactatca agagcatttg acgaatggcc
    49921 tactcttgtt aacagatatg ggatcattaa acaccttcag tgatttgatt tatgaagaga
    49981 ctggcattcg gacgaaagct atcacgatga caagtaccat gattgtgata gaagcgatac
    50041 ggatggcaag tgttggaaga agcttagaag atatttatca aaatatccag ctttcctttg
    50101 aatcgatcgt acgtgatcag ttcaaagcgg aaaagtctga aactttacag cgtaaaaaag
    50161 ccgtaatcgt gacatgcttt actggtgaag gagtagctgc caaattatat cagcgtatta
    50221 tgccagtgat taatcaaaat aaggtagaac tgatccagat gcagttttta gaacgtgaat
    50281 cattcaaaaa acacatcgat gccttattgg aagaatatga gatcaaagca attgcgggaa
    50341 cagttgatgt tgattatcaa aatatcccat ttttttcagc ctatgacgtt tttgatgatg
    50401 aaaaactaaa tattttaaag cgtatagctg atgatgagat cccgacagct aaaattgtcg
    50461 attcacttgc tggatcatta agcgatgtct tttctattga tgaactgatc cgttcattgc
    50521 aaaagacagt tcagcagatc cagaccgatt tgtcgattat cgtggaacct ggagtagata
    50581 ctggaatcgt tatccattta gccttcatgt tagacggatt gatcaaacaa gaaagccatc
    50641 ggcaatttga gaaactgtct attttctcaa aaatttatcg tttggaaatg gatatcgttc
    50701 gggcaaactt gatggcacta gaacgaaaat atcaggtaaa aattccagaa gatgatatcg
    50761 catttcttac acaaatgttt cttgaaaata aaataaaaaa atgaacacac taatcatact
    50821 gtttacacac taaacatctg tgtgcgcact ataaaaaaga gctaaaaagc atgaaaacgc
    50881 tatttcagct ctttttttta ttggcatgct tcttgcgtta tataaaagtg taaaaaaata
    50941 aaaggagtgt taaaaatggc aaaaaaaaca atcatgttag tttgttcagc agggatgagt
    51001 acgagtttat tggtcacaaa aatgcaaaaa gcagcagaag aaaaagggat ggaagccgat
    51061 atttttgcgg tctctgcctc agatgcagat aataatttag aatctaagaa tgtcgatgta
    51121 ttattattag gtcctcaagt acgtttcatg aaagctcaat ttgaacaaaa acttgcgcct
    51181 aaaggtattc cattagatgt catcaatatg caagactatg gcatgatgaa cggagaaaaa
    51241 gttcttgcac aagcagaaaa tctaatgaaa taaatcaagt atgcagttgg gggtataaca
    51301 acgtggaaga tcaacaaaat ctagaagcca tcatggggtt aatcatgttt ggcggtaatg
    51361 caaagagcga tgcaatggaa gcgattgcgg ctgctaaaat cggtgatttt gagttagcgg
    51421 ataggaaaat cgctgatgca gaagaatcac ttatacaagc gcatcatgca caaacagaca
    51481 tgttgacaca agaagcacaa ggaaatcata tgcaagtgac attattgact gttcacagtc
    51541 aagatcactt aatgacatct attgcgttta ctgatttagc aaaagaaatc atcgaacttt
    51601 atcgtcgcgt atacagcgaa taaatacaac ttcttttttg aacggaagaa gaaaaatttt
    51661 tgaacggatt ttttattttg ttataaggag aactctcttt atataaaaaa cttataggag
    51721 gaagtaaaaa atgaacggac tatcaagttg gatggagaaa tacatcctgc cggttgccgc
    51781 aaaaatcgga gcgcaaaaac atttagttgc tttgcgtgat gcttttatcg gcacactgcc
    51841 cgcaacaatg gcgggatcag tcgctgtaat gatcaatgca attattcgtg atttaccgca
    51901 acagtttaac agtacctatg ctgacagttt agcagcagac aaaggaatct ttgccgtatt
    51961 gaatgtcatc atcggaatca atggttttgt ctggaacggt acactagcaa tcgccggatt
    52021 gatctttgcc ttttcttggg ggtataatct tgcccgagct tatggagtaa atgatctagc
    52081 tggggggatc gttggtttag ctaccttgat tcaaggtatt tcctttggtc cagcattatc
    52141 agcagcatta gatgtgaatg ttccttcaaa tgtcgcagat gcaatcaata gttctgagat
    52201 tggtgcaacg attgcagatg gcacactgtc agttggatta tggggttggc taaaactaga
    52261 tcatttgaat ggaaacgctt attttacagt tatgatcatg ggagctattt cagtcattat
    52321 tttttgtaaa ttgatgcttg ccaacattac gatcaaaatg cctgattcag taccaccagc
    52381 agtatctaaa gcatttgcag ccattttacc agcgacgatc gctttatacg tcattgctat
    52441 tatcaattat atcgtaggtc gtgtagctga tgggcaactg atcattgatt tggtgcaaaa
    52501 atatattgca gaaccattcc taggattatc tcaagggttg ggagcagtat tgatcgtcac
    52561 agtatttgtt caagtattct ggttctttgg tattcatgga tcaaatgtat tggcaccagt
    52621 gctagaggga atctggggac aagcacaact aatcaatatt gatattttcc aaaaaggtta
    52681 taacggaaaa actggtacac cagccgtgtt agatgcaatc gaagatggaa aagcttatat
    52741 gtgggtccgc ggatcgttcg atgcttttgc atggtttggc ggatcaggcg gaacgatcgt
    52801 cttactgata gctatcttga ttttctctaa acgtgcagat tatctaacag tagctaaatt
    52861 atccattggg cctggtatct tcaatatcaa cgaaccagtg atgtttggtc tgccaatcgt
    52921 attgaatgca atcatgttta tcccattctt gattgctcca gtagtagcaa cagcaattgg
    52981 atggacagct acttatcttg gcctagtcgc tccggtatcg cagcaagtca catgggtagt
    53041 accgccgttc ttattgtcat tcttagcaac aggagcggat tggcgggcac cgatcgtaac
    53101 attagtatgt atggtcgtga ctttcttgat ttgggcacca tttgtcatct ctgcaaacaa
    53161 aatggaccct gccgcaggcg aacaacaata agatgaacgt gaggagcggg acgctttaag
    53221 tttcgttcct cataatgaat tttcgaggag gaactgaaat gattacaatt gccagtgcaa
    53281 tgcaacaaga tgaaatcaaa gaattgttag aaggatacaa tgaagaagga atgactttta
    53341 catttaaaga aaaacaagga atcaagctgt tctttgaaac aagtgcagaa gatccggaag
    53401 ttgcggctaa aaaagcaaag gaactaatca aagcacagcc ttggggaaca gtcctttatt
    53461 ttcaatcaac agctgtttag aaaatggttt aaaaagatag gatggttgag atgataaaaa
    53521 acggtttatt tttatgtagt attggttctt tgatcgtgtt gtatagtttg aatccacatt
    53581 cgcaaaggta tgatttgtat agtatggtca caggtctttt tctaacagga ttaggaatct
    53641 atttcttcat gaaggggaaa aaaagagaag aagcaaagga gaaaaataac taatggaaat
    53701 cacgaaaaaa ttaaatattc cagcatcgtt tttttacgat aaagttatgg actctgtcct
    53761 tttcgatgta cgtaaagcga ccggcaaatc agtaactaga aaacagctga aaaatttgga
    53821 gtacgtcaaa caattctcgc aaaataaccg ggctcgcata aaaatcgaag aagtcattga
    53881 aaaccagtct tataagtttc gtacatcgac aacgaagaat gaattcgtcg ttcattatca
    53941 aatcaaacca ttggacgatt cgacctgtga ggttcattac acagaaaaaa tggagtctta
    54001 tggcatgctt caaaagttga atgatatgct tttaggaaca atcttgatgt attttaaacg
    54061 gagacgtttc aaaaaaatgc tagatatgat cgaagcaacg tattaaatca atgaaacatg
    54121 gtactattta gttattgaaa aatgaagtaa gctagaagga atttttcata ggaagataga
    54181 tctttcctgc gaatcctcac tagctttttt gtaggaaggg gcagcaaaat gacaaacaaa
    54241 gtaagagagc taggtgtatc ttcgattgag gaaatgtttg atttagccac ttatgcattt
    54301 aatggggcag atacacctga aaggcgagaa cgttttcgta cacttgcgga gaattcttgg
    54361 aattacggat tttttgatga ggaggatcgg ctaacaagtc aagtcatggc gacccctttt
    54421 ccagtgaatt tttacggaca agagtaccta atggccggca ttggctatgt tgcttcttat
    54481 ccagaagcaa ggggacaagg cgggatcaat cagatcatgg aaaagatcct tgaagattgc
    54541 cgtgatcgta aagtaagctt atcttatctg gcgccattct cctatccctt ctatcggcgc
    54601 tatggctatg aacaatcatt tgataaaatc agttatcaac taaacagtcg tgatgtgccg
    54661 tttatcaaaa aaaaatctgg aaaaataaag cgtatggatt ttgaagatgc aaaagctgca
    54721 atcagaagaa tttatgaaca gatgccggaa aaccaacgag ctggtctgaa gcgcgaagac
    54781 tggtggtata cgtataaatt caagcaaaaa ggcaattacc agtttgcttt gcattttgat
    54841 gataatgagg aagcaacggg atatatcgtt tatcagcttg caacgtcttc ctttattatt
    54901 gcggagtggg gatttttgga tcatgatgct ttttgcagcc ttgttcgctt cgtaagttct
    54961 cacaatggtg cttttgaaac atttgaatat tcatgcggct acggcggtaa tgatcaaaat
    55021 tatctattag aaaatccatt tgcttctgta atgatcacac cttatatgat ggcgaggatc
    55081 gtagatattt ctttgttttt ggaaaattat ccatttaaaa agagacaagc tgcatttgcc
    55141 ctacaaatag aagaagatca ttatgcgaaa tggaatgaag gaatcgtgga agtaacaatc
    55201 gaaaatggaa aaaatcatct ctcaaaagta gaacaaacaa aactcccaat cgttcgagga
    55261 agtatccaac atatcacgca gctattatta gggtatcgta aagtggaaga gttggtattc
    55321 caagaaagaa tacaggtgac aaaagaatat gaagaaacgt taagctcaat cttgccacag
    55381 caacagccaa ttcttaatga ctatttttga taaaaaacgg gtgatgacaa tatttttgtc
    55441 accacccttt tgtttttcat ttggcatttg aaggaaaatg ttaaagtaaa tcctcttttt
    55501 tcgtaaattt gaattttttg ttttcttttg ttttagggtt ttcttttttg tggtcttgga
    55561 ttgcttcttt cgtaatcttt tcccgatttt ttttagatga atctaagcgt tctgcgtata
    55621 ctttttcttt tggcttatca ttcattggaa aggacctccc tacattattt cattctacct
    55681 ctagtttatc atctgttaag gactagtgga aacgttttgc ctaacttata agctgatttt
    55741 tttagcagat aagcttgcaa gtaaaaaaca aacaagctac aattgattta ttgaaattgt
    55801 tcctgtagct cagtaggata gagcaatcgc ctcctaagcg ataggtcgct ggttcaattc
    55861 cagtcaggaa cgtataaata acccctgtat cctttgcggt acaggggttt gttttgtatt
    55921 tgtgccaaaa atgtgccaaa ctagttatca tttttatata aactatcgaa cacagtattg
    55981 attttttctt tatcctgatc ttcaagctct cgaacaatat gaagataagt agacattgta
    56041 gtttctaaac tgctatgtcc taaacgtttc gatacgctaa gtatatttac tccttgataa
    56101 agtaaaatag atgcatgtgt atgccttagt ccatgcaaag taaattgctt ttcaagccct
    56161 aattctttta attttttgcg caaatatttg ctgacggcgt ttgatgaaac taatccattt
    56221 tttaagttga aaaaaacaaa attatgaggg tttttgattt caaaattctc gaataattct
    56281 ttttgattga ttttaaactt ttttaagaga tcaatcgtat gtttatcaat ggaaattttc
    56341 ctatttgaag tttcgttttt tgtttttccc cattcattta atttataatt ccatgtttta
    56401 ttaatagtga tgatttgatc ttcgaaatcg atatcttccc atgttaatcc tagaagttca
    56461 gcaaatcgca ttccagtggc accagcaact aacacgagca taggagaaga gtactgcgca
    56521 gaaaggtttt cttctacgag tttcattaaa gatttaaatt gatcataatc taaatattta
    56581 tcttcttttg gcttcaatga atcttttcct ttgattactg cttttctagt tggatcaaac
    56641 ggaattaatc cttcttctac ggcatctttc aacgacgctc taatatggtt attaaactta
    56701 ataacggtag attttacatg gtctttagca tacttattta aaaagcgttg atatcccact
    56761 ctatctaaat cagaaatcaa taccgctggc atatatttct ttatgttcat taacgtatct
    56821 tcgtattttc tataagtgat aggagatact gttccttctt tataaagttg catccaatcc
    56881 tcaaagtaat ctgataataa tagatttttg cgatcccctt taaggccttt gttcaagtta
    56941 tattctaatt cgttggcggc atcttttgct tctccttttg tcttaaatcc tgattttctt
    57001 atcttcgtgt attttccgtc atcttttttg tatgaaattt cgtattgcca actatttcca
    57061 cgtttgacta atcttgccat aattgaatca gctctctttc tctgatacaa taggaactat
    57121 aaagaagccc attgtttggg ttttattatt ttttgcacat cctttttgct tgccggcgca
    57181 aggatgtgct atttgtcgtt ttaatggtat aattggatgt gtattagatc atccaatcag
    57241 aggtgtttat tatgattaaa gtgattttta aaagaataac tgaacttcca gagtttagat
    57301 tatttgttga ttattggtat gtctggtttt ttattgttgc attgctgata ggtctagatg
    57361 tcatattaga aagaaaactt aagtaagagt tgagttaata aattgaattc ctaaatgaga
    57421 tattttgtag cccatatttt gtttgccgac atcacctaat actatggagc taatcgtttt
    57481 ttctaacaga ccaaaatcag ataatctatt taagctacca tctattagct cttgtgaata
    57541 gccagacatg ctgatatttc cagtgaaatt ttcgtttcca cgactcatta agcatgctaa
    57601 caattgaatc tcaatcgatg ttaattgatt cagtacaaga agataatact cctcgttatc
    57661 ccatgaagcg ttgttaattc gaagtaaaca attcttataa agatttttat acatatctat
    57721 tttattttgt gcctttgatt tttctacctc gtcatttatc ccttcgaata ttccaatgag
    57781 ttggtccttg ttctctaagt tgaaaacatc ttctggaatt tgatcgcgaa tgttttctaa
    57841 tgattcgttt aaatttttgt agaatttctc gatgcgtttg aaacgcttct cgtttttaga
    57901 cccaaaataa agtgactgca caacgccacc aatataagga attgctccaa taccgccttc
    57961 aactattaat tcaagatatt cttttttggt tagtttttca tttctattat ccatagttac
    58021 ctcctatttt gtcatttcct ttacaagcat tgtgactcct gatggcactc ttttgtatag
    58081 gttttattat tgtgcacaat ctaactctta gtggggcgtg gttcatttat tttttactta
    58141 ctttactatt aatcatatcc cattgagcat aggatttttc tatgttcgat cctcttgaac
    58201 taatttctcc tgtaaaagtt actttatcgc cctttgtaaa ggtatcagca ttgtttaagt
    58261 ccttcgcaaa aattacatat gaaactttag gtgttgcaga aatatcattc caacttttat
    58321 catcgtactc tttgtctgct ataatggcta ttctagtagt cattgattcg ataacagttc
    58381 cttcaaaagt atatgtttta ccactaatat atttagtaaa agctgtattt tgctcagtag
    58441 ctggagtaat agaataataa ctatctaaga aagcacccca gtcatgatta tttgtctcta
    58501 aaaattcttc aatttgagtt aggcttttat tgctgctgtt agatgtagta gtttgagtat
    58561 cagatgatga gctttcctgt ttgttattgt catttgcctc gtttgatgag gaaagcgaga
    58621 aacctacggt agtaaaaata atcccgataa taaggagaat cataggtgtt ttcttatttg
    58681 tttttttgat aaatcttaca attaacatga ttaaacctat aataaataaa acaataccag
    58741 taaaaccaaa tataccgtcc attaatagat cctctttctc ttttgatatt gaatattata
    58801 aaccaagcaa ttcttttttc ttaaaatcga attcttcttg attaataatc ccttcatcta
    58861 acaactcttt atacttttta atttcatctg cacttgaaat ttgtgtttct ttggaattat
    58921 tatctaatat agattgttgc ttctctatat atttttttaa attttccatt tcattccaat
    58981 atttttttgt aaacataacg gtgttttcat ctttcgtggc cgctaatata cctccgcgac
    59041 tttcgtttcc gcctagcaag gtaaattgta tataaccatt actcatacct ggttttttta
    59101 attgtacggc tgatatattt ttaaagggga tagttttttc tcctttaata ccttgattaa
    59161 ctaaatttat aaatcctttt ctaataattg aaatagaatc atcagttact ttaatcaagg
    59221 tttttcctgg agattttatt aaaatctctt tttccatttt ttcttcctca tttctgtgat
    59281 atgatttttt ttgtagaagc tcatatatga ggaaagagcc cgtgttgcag cacgggcttt
    59341 tttaatgttt tgctgaaatc ggttttttta ataattcttg atgtattcat acatattgcc
    59401 ttgcgtaaga atattttttc ttaaaatagc attggcagac agcataacaa gtgtatctga
    59461 gcttattctg aatagaataa tactccataa atttttcgag attaaactga gattcatcag
    59521 tcagttcgtt ctcaatatag atatttaata aaattagaat agctatttta tctgcttctg
    59581 tttcaaattt tgagtgaaaa gttgtagaag tatcgtacaa aactgaaaat tcaaaatgag
    59641 aggcgctgaa atgtgcaagt tcatgagata aatgaaaggc ttctgcagtt tcaccgtata
    59701 gattttcatt caaaaaaatg attctgggtt ttggatagta gaaaccaggc tctttcatct
    59761 ccatataaac tactttcaaa ttgtattcgc tcaacatttc tttcaatttc aaatacatac
    59821 aaaccatcac tccaactatt cattttcctc taaagcttta gcaattgcaa tcgctttacg
    59881 cattgtctcc ttagatattt cttttccatc aaaagaaaaa acagtatcgt cttctgataa
    59941 atccacatgt ttaggggttt ctctttcttc tctacctaga aggtagtcta cagagacatc
    60001 gaaatagtca gcaatccgac ttaattcatc tgaattagga gtattatttt tccactttgc
    60061 tagataaccg ttagaatatc caaaattaat ttctaactgc cgtatagata tctttttcct
    60121 cttcgccaag tcttttatta tttcataggt attcatcgat aaaacaacct ttctaaatgt
    60181 ttacaaaaaa agtttagaaa aatacgcaga aataatttga ctaattctga gtaatgagct
    60241 atactatatc ttgtaaacag atttaacaac taaaaagaca acaaaaaaca ctattgatat
    60301 gtaaatgcag accgccaaga aagctttaaa atcaatgttt ttatgtctta tttaattatg
    60361 ctcttagtat agagtattac tcagatactg tcaattgaat ttagaaaaaa gttgttaaat
    60421 ttgtttacga atataaaaga aaggaatgag aaatatgaac acaccgcaaa tttttaattt
    60481 cgaacaaaac gaagttcgga caattttagt aaatgatgaa ccatattttg taggcaaaga
    60541 cgttgcgagt gttttgggtt attcaaacac taaagatgct ttgtcgcggc atgtagattt
    60601 ggaagataag atggggtcgc gaatcacgac ctcaggtcaa tcaagagaga tgacaatcat
    60661 caacgaatct ggtttgtaca gtttaatctt aaaatcaaaa cttccctctg ccaaaaaatt
    60721 taaacgttgg gtaacaagtg aagtgttgcc agcaattaga aaacatggag gttatctaac
    60781 tccagaaaaa gtagaagaag ctttgcttaa tccagataca atcattcaat tagcaactca
    60841 actaaaagaa gaaagaactg gaagattaat cgcagaacaa aagattgcag agtacgaacc
    60901 taaaatctcc tatttagata gcatattatc ttctacagat tcagtaacaa ttagtcagat
    60961 tgcagcagat tatgggatgt ctccacaaca gatgaataaa ttacttcata aactaggtat
    61021 tcagaaaaaa gtcggtaacc aatggttatt gtgcaaaaaa cacatgaacc aaggatacac
    61081 aaaatctcat acaactgaga tcccgaaagc cgatggtggc actaaaattg taatgaatac
    61141 caaatggaca cagaaagggc gtctatttat ctacgaatta ctaaaaaaag aaggatatta
    61201 ccctcaaatg gatttagagg aaattggtta gaaaggagtt ttagtatgac tgacattgca
    61261 gaaatcactc aacgagatag agaaaaaatc aaagaatatg tcgaaagttc aaagttctta
    61321 acttacacca tgcttgctga aagatttgga attagcaaaa gctacttatc tttaatttta
    61381 aacggtaaaa agacttctgc agaagcaaac agaattatag attcgattat cactatgtac
    61441 gaattgtaaa gggaggaaca gctaatgcaa tatctagaag caaaaatccc aattccagaa
    61501 ggctatgtaa ttatctccca agtggattat gaggagttaa aaaaagctga tgatactggt
    61561 agatggatga cgttgccaga agtgctagaa cggattaaca gaaaatatga ttggtttact
    61621 tctagagttt taaagaaccc aagatataga aatattatcg atatcgagaa aaacaaaaat
    61681 ggatttgtct attatccagt tgaaggaaga gacacatatc tatttttaag aagtaaaaca
    61741 cttgaatttt tagaaacaaa tttttcggaa atcttaagga ggcaagcgga tgggaaaatt
    61801 taatagagcg ctagtgttca gcgcaccgct aatcatctac gctttaggac tttggggaag
    61861 caggcaagcg ttgataggaa cgattgttta catggtttgg atttttatgg ggcttgatga
    61921 agctgaagct gagtacagag cgaaaaagcc aaccgagggg gctgactaaa aatgaataga
    61981 aaagagaaac tagaatgtat attactatta ctcagtttaa ttctttcact aatttctcta
    62041 ttgggaagtt tgtatttttg atatcaacaa ttttgtggat ttgggttcta tttagagtaa
    62101 atatcatttt tgtttggtga ttaaggagtt tattaaaatt aattttccct acttcaaatg
    62161 caaggtaaca agatatagca cttttaggag gaatatttag cggtacagct tcagagtata
    62221 ttctattatc atcgactgat cctcctttag caattaattt cttaaatgga gtagctgacc
    62281 atctgcggtt taagttagcg gatgaaagtt ctaaattaac taacatagct ggttctgttg
    62341 aatagttaga aatgattact tttgtataaa tcatatctgc agcgaagtaa gaagcattaa
    62401 gttcaacttc tagttgaggg cgcattcctt ttatttttat ccctgttaaa aacgtactca
    62461 atataaaact tattattgac atccattgaa aaattgttaa gtgaaaaaaa ttcaaaataa
    62521 acaccaccag tttttaacta aattatacca aaaaggagag aagaaataat gcaagaatta
    62581 gtaattttga aaaataaaga agctgtgact acgagcttac aagtggcaga aagcttcgag
    62641 aaaaaacatc aacatgtttt aagagatatt gatgcactaa aaaaagatgt gtccaatttt
    62701 ggacagatgt ttgtagaagg taatgaacca gattcatatg gcagaaatcg acgagttttc
    62761 ttcattagta gagatggatt tttcttgctg gctatggggt ttacaggaaa gaaagctatc
    62821 tacttcaaac aaaaatacat tgaagcattc aacgaaatgg aagatgttat tcgcaagaat
    62881 actgttcctc aaacaattga agatatgatg atctatcaat tagaagaaat gaaagatgtt
    62941 aaaaaagatg tttccatgct taaagatact atgcgaatta gcggacaaca agagtttgaa
    63001 attaagcaaa aaggaaatat gaaagttatg ggagttctag ggggaaaaga aagccgagct
    63061 tatgaagaaa tcagcaaaaa agtattctca aaattttggt ctgaatttaa acgtaccttt
    63121 tcaatcccaa gatatggcga gttacctcgt aagagattcg atgatgctgt ttcatttatt
    63181 gaaatgtggt tgccagaaac tgcgatccgt atggaaattg atcaactgaa cagacaacag
    63241 agactttttg gtgatgacaa tgaatagagc tgaagcgcta agaataggga cggcaattgc
    63301 taattgctgg tggaaatact ataaaccaat catcctaagc caacaacata ttgacaagca
    63361 aaaagcatgg caacaaataa aaaagtgact ccgccggcaa gcaaagagtc acaaagaaaa
    63421 cacatcataa ggagatttta gcatatggaa aaagaacttt ccactctaga tcaatatttg
    63481 attgatcctg attggggcaa gccgaaaatt gaggaaacaa gtggtcgaaa aatcagacga
    63541 aatcttttga cgaatgaaga actagcttgg gatcaagatg atttaggcaa ccatgtaact
    63601 atttgggatc atgtttatct tatccatcta tcgaagcatt cgaataaacc tgaatatatt
    63661 tacgtcatcg aagatggctt gattgatgcg ctagaagagt acgacagaga taacttgatt
    63721 gatatctctt attacggacc aggtaagaaa tacattgctg aaatggaggc agaatttgat
    63781 gagtgaaatc aaagggacaa cgaactttga aaaacttttt agtcgtaagt taaataaaat
    63841 tctcaagaaa aaaggaaatt ttgattattt atcttgggct cacgcgtggg agattatgaa
    63901 aaagaatgat ccacaggcaa cggtaactat taatgagtac aaacactaca gggttgtttc
    63961 tggaactcat caagactttc ttgttgagga atataaacct tttcttatgg atgaaactgg
    64021 gacttatgta tctgtctcag taacggttaa aggacacacg gaaaccgaat tatttcctgt
    64081 tttagattat cgaaaccaac cagttgttaa accaaatgca atgcaaatca ataactcatt
    64141 gaagcgatgc tttgtgaaag cattggctct acacggactg ggattatatg tatttcaagg
    64201 ggaagatatt ccaacaccac ctagaatcga tacaaagaaa ttaaacatgc tagagacgat
    64261 tctagaagct ttcaatgagc agatgggtaa agatatgacc aaaaccttaa tcgaatatgt
    64321 taatgagcag acagataaat tagggctctt agctgataac gttgaaacta ttgaacagtt
    64381 aagctatgag caatgtgcct tgatggagcg agcaatagca gctaagagaa aagaattaga
    64441 taagaagtga tatgagtgtt taaaccatta atcgattcat attcagcggt tctgaaaaag
    64501 ttcaaaggaa aagacataag cgcaaccatc aatgaggaag tgaacattga tcgactaaag
    64561 acgatgtatg acggctacga tggtgatcga gtcattgaaa ttcgttttat tgatcctaga
    64621 cgtttcaccg tacagcaacg aaacttcatc tatgcgctga taggcgatat ttttatcgat
    64681 acaggcatgc caacggactt ctggaaggaa ttcttctact ttcgttttga aggtgtcaca
    64741 gggcgcaaaa taagcctgaa agacgaatcg aatacaactg tgagtgatgc caatgtctta
    64801 gcaaatatca tcttagattt catctttgaa catcatattc ctttcaaaga aggctatgag
    64861 attttacctg cgaatcaaga atattacttc tacaaatgca tcacaaaaag agtctgctgc
    64921 atctgtggca aaacaggagc tgacatcgat cactttgaca aagcgctagg aagacgaaag
    64981 cgcaaagaag ttgatcatgc agagtacaca tttgcagcac tctgcagaat ccatcacaca
    65041 gagaagcaca aaataggtgt gattaatttc aaaaataaat atcaaatcaa agggatcaaa
    65101 ttaaaccaga aaacaatcaa aaagttaaat attggagggt aaaaatgaca gaacatcgaa
    65161 gttattatgc gattatacca gccaacgtaa ggtacgacaa aagacttaaa ccaaatacta
    65221 agttgttata cggagagata acggccttgt gtaatgaaag aggcttttgt tgggcaggca
    65281 atgagtactt tgcagattta tatggtgtga ataaagagac catatcgcga tgggtaagtg
    65341 atttgattaa gtttggatac ttgaatcggg aaatcattta caaagagggt accaatcaaa
    65401 taatcaatag gtacctacga attaatcaat accctattga cgaaaaacgc aataccccta
    65461 ttgacgaaaa agtcaaagat aataatacat ctattaataa tacatttaat aatacaaaag
    65521 aatatataag agagttaccg ccttcgaaaa aatcgaaggc taagcccgtc cgtcataaat
    65581 acggagagta taaaaatgtt cttttgtcag atgagcagat ggagaaactc aaaacagaat
    65641 tccctaatga ttaccaagag cgaatcgaac gactgtcaga gtattgtgaa tcatctggta
    65701 agacttataa aaactatttg gcaactattc gaagttgggc aaggaaagaa aaaagtgaac
    65761 ctaagaacgc aagcagtgga tacaagcgca caggaagacg agagaagctt cctgaatggg
    65821 caatcgacca agaagcctat ctcaagaaaa aagcgctaga acgagctaat agacaatcaa
    65881 aagcaccatt ctaagaggtg gaaaaatgaa gatcgattat ctagaactaa ttaatgaaat
    65941 agcgaattat aaaaagggcg aggaattaga cgtcctgaga gacgtatatg atcaactcga
    66001 agaagctgga attgaaggaa ttaagaatga tcgttcgagt tggagtaagc tcagatacta
    66061 tttcgcactt tatatcgata caacacaatt aagaaattta gcatatacca aattactatt
    66121 tgttgattgt gttaaaggct tgcaaaaaca tcttaatgaa cttgagcagg tgtaatcaga
    66181 tggacctaaa gacatttaca gcacagattg aactaatgca tcaagaagct ttaagacaaa
    66241 gtgtatcgta cgaagacaag tggctcaaca cgttccatgg cggacgtgag agcgcacttg
    66301 atcaagtact caaattactg aaaggagaat gtcgggatgg ataagaaagc ggcaatgcag
    66361 cgaattatcg aattgactta ttcagaagat tggcaaaatg acaaagaagc tgcttcagaa
    66421 gtgatgaggc ttggaagagc gatgtgggca gacaagagca acaagccaag accacgaaaa
    66481 atcgcaattt ggcacggtga caaacttcta gtgataggga cagctgaaca gttagcaagt
    66541 ctcacaggct tgcacgagaa aatcgtgagg aaaagagcaa ggtgtggcta cacagacgct
    66601 aagaagagaa cgtttagata cgtggaggaa tcatcatgac aacagaagaa gtgattcaaa
    66661 tgcgtattcg aagccttcag cgtgagattg acgatctgga acgaacaaag gcagtgatgg
    66721 tcaatgaaac ggcgagaaag gcaatcgatt tgcacataga gaatttaaga agggaaatcc
    66781 atcgattgga ggaatgagcg tggataagaa agcagcaatg aaaagaattg ctgaattaac
    66841 caagtcagaa tcttggcaag aagacaaaga aatagttgca gaagtccaaa agctcggtaa
    66901 atcaatgtgg actgaaaagc ccaaacggag aacgccgaga aaaattgcaa tctggcatga
    66961 tgaccgaatt ttagtaacag gtactgctga acagttatct gaaattactg gattaagcaa
    67021 aaacattatc tgggatagag ctaggagctt atggattgat tcaaaaggac gacagtttag
    67081 gtatgtggag gagagataat ggatctcatt acacaataca gtgacatcat cctcaagaaa
    67141 atcatgatga agattcagaa agataaaaaa tcaaaagaac gagctgaatt agttaagtta
    67201 gaaatggctg aaacaggagc aggagtgcga agtagcaggc attggaaagc agcagcaaac
    67261 attgaatttt attacaacga aattcaaaaa gggttcgatc agatgcgtga gctggatcgg
    67321 caaacaaatt ggagcaagaa acttcatcaa gatcgtttca aatttgtaga gaagtataaa
    67381 gaaatattag aagagtattt gaggaggaca gcaaatgata aaaaaactcg ttcaattcag
    67441 catggattta tatgatatcg aatcaggagc aacactatct gtggaatcgg accatctaat
    67501 cataaatttt ggtggaaagc gccagattat tttgtgggta gttgatgatg tactgtttcc
    67561 agaaattgtt catgatttcg aagaatcaaa agcggttgag tttgaaatag tgaaaaaagt
    67621 aatggaattg attgaaaaat acgaggagga cagcgaatga taccgaagtt tagagcgtgg
    67681 gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa aaacggcaaa
    67741 acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca cgtgcgaaac
    67801 atcgacctca tgcaatccac aggaatgaaa gataagaatg gtgtggatat atttgaaggg
    67861 gatatagtat tagtcagcgt gcgaaatggc ttcgattact tagataataa agtctgtatt
    67921 gtcaaaaatt caatagatta ttccggatta gtttgtgcca ctgttgatga agacttagag
    67981 tatcaaattt ttaacacaga gctgtttgaa gaatacacgt acgaagtcac cggaaatata
    68041 tacgagaata gcgagttatt ggaggaacag cgatgaataa acaggaagca atcaaaaagt
    68101 tagaaagtat taaagcgata ggaaatgatg caatagctgc ttgctataac gagagtataa
    68161 attcaggtat tacgttaatg aaaaaaatag acgaaccgca gaaaccagtt attccacaac
    68221 tcgtggccgg ttggcttgag aaatctacgg acccttttac aaaagctgaa aaaatagcgt
    68281 atttaatcaa atctaaagat ggtgattcat attatttctg tgattggttt gtacgagatg
    68341 gcatagtgac gcaagagcaa ggagaagaat tactcgcttg ggcaacaaga caatcatatg
    68401 aaacactatt gagcctatac aacggctacg aggttgagaa agagccgtta tgggcaataa
    68461 agaatgccga tggaaactat cttactaaat gtgctttatg gggaaaagat ggagtaaatt
    68521 atagttttga atgcaatcca tctcatcgat tgcttttcac tgataaagca acagcggatg
    68581 ctgcagcatt gttggtgaat ggaacagtgg aagaggtggt agaaagatga aactaaaaga
    68641 cggattttac gctagcagtc acggtatcgg cggtttaatg ctagatatgc cgacaaagaa
    68701 ccctaaaaca cgcaagaaat caaaattcaa agtcggtgac atggtccgct gtgaagcaga
    68761 agagttcgtt tatccgttca gaggatacgt taaaaagata ctgtcaaact cagcaatcat
    68821 tcgtattgaa aatacaatga agtgtgatcg ttcgactgca aaacacaagc attatttagc
    68881 tgtagcgaga ttagttgaca tggaagtaat caagagcaaa taaaaaaagc cggatcgctc
    68941 cgactgattc aataaatcca acacatttat tatatcacat aaaggagcgg tttgacttga
    69001 tgcaattgtt acgagaggta gatttcaaac agacaagatg taatgcgaga gatgtgctga
    69061 agaactttcg gcgtttggag cggatggcag gtcgctcttt gatagatatt aagtcgccga
    69121 ttataacgga tatgccgaag gcaccgaagc atggcaataa ggcagaagac gcgattattc
    69181 agatgatgga tatagaaacg gagagagatg cgattctagc ggctttgatg gctcttagtc
    69241 tgattagtcg tcagatactc tactacagct tctgtgtgcc agatagcttc tcaaactaca
    69301 gaattagccg tgaagtgggt tattcagaaa gaagtataca acggatgaag tcggaagctc
    69361 taatagagtt tgcagaagca tataaacacg gaaaaataat tgcttataaa taatttggcg
    69421 gttttttggc ggaatgatgg cggtttttag acatttacca gtgttattat ggtagtgtcg
    69481 aaagattagg aaacaggatc gacaaaataa acgaagggag gcaatctccc tcatcgtttt
    69541 aaacttcttc ttagatagac agcaactaat tatgaagaaa aggatgtgaa ttcaactcct
    69601 cctaaattct tcatcggttg ctgtttattt tgattagaca gcagcactca caaaactaaa
    69661 catgttcttg tcgtctttca actgctgctg tctattaatt tatgtattgg agggaagaaa
    69721 aatgaaatta tcagtagaag gcactccaga agaaatacaa gaattgctcc aagctattgg
    69781 tagtagcaag gagcaaaagg taagtgtttc taattcagat attgacggta tttgcaattg
    69841 tttaaacaga gaaaaaatat ctaatcctta ctaagagaaa atatgttcgt taatgaattt
    69901 ccaattttcc tctgtagctc taccttgata atttttttgc agttcacaga tgaataagga
    69961 atcgttatta tcaagtactg tatttaattt ttcacacatt tgatttggat tcaaattagt
    70021 tttaactaac caaaaagatt tttgtagctt tatatatgcc ccaaattctt ttattatttc
    70081 aaatacttta tcatactttt gccctgggtt attaagatca taactaatca tgtagggttt
    70141 atacatgttg tcttgcacat ccttgtttta tatttcagcg gaccactcgc tgataaataa
    70201 aattatacgc ttagtattta ttttcacaat attaatttat cgtaataact tttgagtaaa
    70261 tagaacaaaa aacctgcact agtttccgct agtacaggca gtgactatat cggttgataa
    70321 tctagcatat attaaaaata attgcaagaa aagaaattta tagtggttta taccaaatta
    70381 tcaaagcaat gttattttgt tgctgtctat tgttttttaa ttattcacac gataactaaa
    70441 ggtgggtgaa gagaaatgat tccattaata atttcaattt ttgcgctctg tcttaatgtc
    70501 tatatgattg gatttaaaaa tgggcaaaat aaaaaatagt agcagccaag aataatttta
    70561 tagtgtcact gtggcggaaa gggtagacgc taagcatgtg tgctaggtca atgcttcggc
    70621 aaccatgcaa ggttcgattc cttgccagcg acattaaatg cctatgacgg ttacgactac
    70681 cgaaaaaaga tcgttaagaa gctatacggt gctacgtacg gcaatgtagt aagtgtgcta
    70741 tctgtacacc accaagcttc ggtcactgtg gcggaagtag aagacgcagc ggtaaatggc
    70801 gagtagcctc gtgagagcct ggtaagttct cgtgagtggt gcaatcccac tccagcgact
    70861 ttaagcaacc gaggcatcgg cggtttaaaa atataggggt gcgcaatttc gtacgcgttt
    70921 tgtgcatcgt gcaagttact attacatatt agatcactct ttgagtggtc tttttatttt
    70981 tgcacaaagg aggaaacaac aatgtataga ccacaatact tagaacagaa gtatgaagta
    71041 atcactgttc ataaaggtaa tggcgaaaaa gtatatgagt atagaagacc aataaagagc
    71101 gatacatata aacgaaagga aaacaatgaa gttattccat tgtatggcaa aagaatagct
    71161 aagcattaaa taagattgcg aaaggagacg gaacatgacc gaggaattct atagatggct
    71221 attacagttg acaagagaag atcgtttggt taagttctat cagtctccta aatggcgcag
    71281 gcttagagag aaagcgatga aacgagatca ctatgaatgc caagagtgta gaagactagg
    71341 taagtatcat agagtagaga acgttcatca tataaaggaa gtcaaggata gacctgactt
    71401 agctttagat ttagataatc ttatttgttt atgtgttgaa catcataatg aagttcatgg
    71461 cagatatctt acagcattag ataaacaaga gaagaagata gaaagctttg ctaacttcga
    71521 tgcaagtgaa aggtggtaag tgcatgatca tcaatgataa tggcagagag tatgatacag
    71581 aaaagattga agagtattca tcttatactc agggattaat taaacgtttg atatacgttc
    71641 gctatgtagg tattagggat ctgttatcag ataactgttg tagtaaatac aaagtgaatc
    71701 aagtaagaga agcgttgaat aaagataata acgtcgaaag aataaaaaat gtttttggat
    71761 atagtattga agagattaat tattacattg acttcgctga agctttcatt ccgatggtga
    71821 gataaccccc ccttaaaata aatcgcaaat tttttggggg tgatgaaacg gagggggctg
    71881 tcaggaaaag agattttttc gaactttatc atgaaaggag ggctaaaatg tttaaaaacg
    71941 aattgtctca aaatcgctac agagaaaaat tacgccgctc tttaataagc caattggaaa
    72001 gtcagaaaac aaatattgag ccattcttag ataatgttga tcgttatatc agtttatggg
    72061 aaacggcgat atcactggaa gaagatatat ccgagaacgg cattagactg gagaatggta
    72121 aaaagaatga atcagtagcg ttgcttgttt ctgtcaacaa acaaatggga ttgatgttgg
    72181 ataaacttgc cattactcct gaattggtag gtgaagcaaa tgaatcaatt cctgagttat
    72241 aagcatattg aaaattggtt caaagctata gaagaaggca ctatcaaggt atgcaaagag
    72301 caattattgc taaaaaatta tctagaagaa agagtcttta ctagagaaga tatttacttc
    72361 gataagcaga tggtagagga ttcaatcaat ataccagcac aatactttcc attcgaatta
    72421 attccgtggg aaaaatttct acaatgtttt atttatggtg ttcgatggaa aaaagataaa
    72481 acactagtgt tcaatagata tctttcatta atgggacgtg gtaatggtaa aactggtttt
    72541 gcttcttgga acaacttctt tctactaacc gctaaacacg gtattaaaaa ttatgatatt
    72601 gatatctatg ccaataatga aagccaagca aagactagtt ttgatgatgt atttaaagta
    72661 attaaagatc atcctgattt agataaaaaa gtatttaaag ctacgaagga agttattcaa
    72721 aatatcgcta caaacagcaa acttcgttat aacacggcaa atgctagaac aaaagatggg
    72781 aagcgaccag gtgcaaaccg ctttgatgaa attcacgaaa atgaagatta ttcaatgata
    72841 aatgtggcta cttctggtgg tggtaaaatt cgagattata gagaatttta tgatacaact
    72901 aatggtcatg ttcgtggtgg tccgcttgat gacattatag aagaatcaaa aatgattctt
    72961 tctggagaac ttggaattga caaggatgga gcagaatttt ctagtttgtt tccatttatt
    73021 tgtcgcttgg ataacgataa tgaagttgat gatcccgaca tgtgggaaaa agcttgtcca
    73081 actattaatt acaatgcaga tttaaaacgg aaaatgtttc aagaatactc tcaaatgcaa
    73141 cgtaatgctg gtttaagact tacgttcatg accaaacgaa tgaacagacc tatggaagat
    73201 acacgatttg ctgttgcttc atatgatgat gttctgcata cgaaagaaaa agaatttcct
    73261 gaaaaaatgg atgaagtgat aggaacagtc gattttgctg atagacgaga ttttgccagc
    73321 gttgggttgc taggaaaata cgataaagat gtttatttta cacaacatac ttttatccac
    73381 gaatcagccc ttcgattaca aaacatcaaa cgagaggtta tagatatttc tatagatcaa
    73441 ggaaaatcac agatcgttca tggaaaaaat atagaagctg attatattgt aggttggttt
    73501 cttgaaatga gtaataaata ttatattaaa aaaatcgcta tggatatgta ccgtgcaaaa
    73561 atattgaagc ccgctttaga agaagcaggt tttactgtgg aaattgttcg aagcggatct
    73621 gttacacatg gtatgttaaa agatctggtt gatgaccttt ttattaatca acgtttattt
    73681 tttggtgacg atgcgattat gcgttggtat tgcatgaatg tatatgaaga gcatatttct
    73741 aatggaaata tacgctatga aaaaatagaa cctgaaacta gaaaaacgga tggctttttt
    73801 tcattccttc atggtttgaa ttttttagat gatatttatg attctgctcc tgtaacagtc
    73861 acaaatagct cagtagaaaa tacaggaact ggatttactc ctctagtatt ctaacttgaa
    73921 aggaggtgag aaagtgggga tttttcaaaa ggcggtagga tacttcacaa aaaaagcaac
    73981 ggttccttta gaagaatact tttgtaaatt gcaagttgat tttgtgtatc gaaaatttgc
    74041 gattgaaact tgtattgatt tgattgcaaa tgcgatgagt aaagcggaat tcaagtcata
    74101 tgaagatgga aaaaataaaa agaatgatct ttactatagg ctgaatgtag ctcctaataa
    74161 gaaaaataat gcaacagaat ttagaaaaaa actgatcagg agattaatat tctacaatga
    74221 agtattgatc gtttctccgt ctaataattc tagcgaaata tttattgcgg atagttggga
    74281 tgtcacagaa tatgcattga aagatgatgt gttttctcaa gtgcaaatta acaacatagt
    74341 ccttgataga gaatttctag aaagtgatgt tatctatata aaatacgcag atcaacaaat
    74401 taggcaacta gtcgatgcgt attatcaagc gtatgggaaa ctcatttcta gtgccatgaa
    74461 tgtttacaag cgctctaacg ctcgtagata cgtactgaaa gggaatttat tccgaccgca
    74521 agacaataca acacaagatc aaatcaataa aatgatgaca tcacaattta aggcttttat
    74581 ggaagctgat aatgcaggtg cggtatttca attacaaaat gagtacacat tagaagattt
    74641 cagcggaaac ttccaaagca attcaagaga tataaaaaac ttaatagacg acatctttga
    74701 gatgacagca gcagcgtttc acgttccgaa aaacctacta aagggagaca tgagtgggtt
    74761 atcggatcaa gtggacgctt ttttaatgtt cgaaatcata ccgattgctg aacttattca
    74821 ggatgcgttt aacgctagtc tctatgaagt agaagaatac ttgtcaggga attttgtacg
    74881 tgtggataca actatgatca agattactag cttcaaagat ttggttgacg ctattgatgt
    74941 aggcattaga aatggagtat ttacaatcaa cgaaggaaga gaacgcgttg gaaatgatcg
    75001 ctctgataag gcgatggcag atgaaatatt tataactaaa aacaatcaac aagtatcgaa
    75061 aggaggtgag gcgaatgacg acaatgaaaa catttctagc agtaaagaat gaaggcgcag
    75121 taccgcaaat ttttattcag ggatttattg gttctagttg gttctttgaa gggaatactg
    75181 acaagggaat caaaaatatt ttggatagtc taggtgatca agaagaaatt gaagtagtaa
    75241 ttaattcaaa cggtggagac gtatttcaag ggattgctat tgggaactta cttaagtcaa
    75301 ataaagcaaa agttaacgtt gtgattaacg gcttagccgc tagtgctgct tcaattatcg
    75361 caatggctgg cgatactata aaaatctaca acaatgcaca attgatgatt caccgcgctt
    75421 ccacatacgg agaaggaaat gtcgatgact tccgtacgat tgctgaccaa ctggaatcaa
    75481 ttgataaatc ggtaaaggct tcatataaaa cacgattcaa tggcacagat gaagcattgc
    75541 aagaacttct tgaaaaagaa tcgtttatgg atgcagaaac agctttgagt tatggattgg
    75601 tcgatgaaat tatcgatgca gaaaatagct caggtactga agctaaaaaa gaacaaagcg
    75661 ttgaagaaat tttgaatgac gttaaagaaa aaagagcaga aaaaattgct gcatttacag
    75721 cagcattaaa taaaacattt ggacaaggag atgtaaaata atgacagtta aaaatttaaa
    75781 aggtgtaaca gctgcaagcg accaattgat gaaagctttt aaagatggta acgaagaatc
    75841 ttttagcgca gctatggtaa gcttatctaa ggaaattcag gataaaattt tagaagaagc
    75901 aacagcaaaa aatcaagatc aattagtatt aatgaaccgt ggtcagcgtg tattaactac
    75961 acaagaaaca aaattctata acgaagtagt gaaaaacgaa ggttttgcag gggtcgaaga
    76021 attagtgcca gctactgtat ttgaacgtgt atttgaagat ttagaacaat ctcatccact
    76081 attgcaaaaa attacttttg ttaacacaac tggtgtaaca gaatggattg tgtcacgtgg
    76141 agtcaatcca gcatggtggg gtaaactttg cgaagctgtt aaaaaagttt tagataatgg
    76201 ctttgatgta attaacatga agcagttcaa gctatcaggt tatattcctg tatgtaaggc
    76261 aatgcttgat ttaggtccag tatggttaga tcgttatgtc cgtactgttt tagtagaatc
    76321 attgagaatt gcattagaac aagcaattgt tgatggtact ggtaaagata tgccagtcgg
    76381 aatgatgcgt gacatgagca aacaaactag cggagaatat gctgaaaaaa aagcagaacc
    76441 tattacagct ttagatgctg taactatggg cggtttgatg gcgcgactat caaaattcaa
    76501 tatcgaaggt gtgaatgatc cgatttatcg taatgtgaat ccttctgatg tggtcctaat
    76561 tgtgaatcca actgattact ggtctaaagt attcccagct aagactgtac taactgctaa
    76621 tggagaatac gtacaagtat tgccagtacc agtttcagat ttgcagtcaa cggctgtgcc
    76681 agaaggaaaa gcagttattg gggtagcctc agattacttc atgggtgtag gatctacact
    76741 aaaaattgaa gcttcagatg aataccattt tgttgaagac gaacgcattt atctagctaa
    76801 acaatatgca aacggtcaac ctaaacgtaa cgatagtttc attgtattag atattagcgc
    76861 tttgggaact actactacaa ctacaaaacc aacaaccaca acaactacaa cacaagcgta
    76921 ggtgatcaga atgaagtata ttctttgtca gccggcaatc aatcggttta aatgggagct
    76981 tgaagtttgt ttaactaatc tgaagaaact aggaatcaaa gatatcgtat tgcttttcag
    77041 cagacacgat gatcagattc ctattttttt tgagaaggaa tatggtgttg aagttcatgt
    77101 gtacgatgat ctgcgggacg acaaagagta tattccttcg attaaaccat atttatggtg
    77161 gaaatattta gaagaagatc attcgcgtga ggacgaccga tatttctata tcgattcgga
    77221 tgtcattttc aataaaagaa ttaatttgcg caaattgcct tctaaagatg atgtttggta
    77281 ttgtagcgac tgctgtagtt atctaagtct tgattatatt agaagctgtg aaaacggaga
    77341 aaatattcta aaagatatgg caaacattgt aaatgttaca gtagaatctt tggaaactat
    77401 aaacactaat tcaggaggcg cacagtgggt tattaaccgt cctaaagcga attattggaa
    77461 aaaggtttat ctggattcta atcggctata tcgctacctt agagggcaaa aaacaaatat
    77521 acaaatctgg acagccgaga tgtgggcaca gctttggaac atgatgtatt tcaatattgg
    77581 tcctaaagtt cacgaggaat tagacttttg ttttgctact gatccaatag aaaaagttaa
    77641 agaagtaaaa atcttacaca atgctggagt aacaacaaac gatgaagatt tatttttcaa
    77701 agggagatac gtgacttcta cgccttttga tgaagattta tcatttgtaa acaagaaaaa
    77761 atgctcttac gcatatgtta aagcaattaa ggcggtggtt agatgacgcc tgaacaagtg
    77821 actgaagaat tgctaatagc tgtgaaggat aatatttacg ttacctggaa cgaagaagat
    77881 gagtcaatta aaaagatgat agctaaaaat gctgtttatc ttcaaagtaa agtgagtaca
    77941 acactttctt tttctcctga aagcttagaa tacggattgc taatcgaaag atgtagatac
    78001 gactggaatc gtgctttaga tgagtttgaa caaaatttcg ctagtgagtt attaggtttc
    78061 attcaacatt atgcgctaca agaatatatt gcaggtgatg tgaatggcga ataatcgtag
    78121 actcgaagaa acattcaacg atggttggtt aaagattttg acgcaaacta ccaaaagaaa
    78181 tgaactagga aaaaagattg gtgtagaaga tacagaaatc acttctttaa aatttagaaa
    78241 tctttccatg agagatagtg atataacagc tatggatgcg atgggatcga aattaactaa
    78301 gaaagtaaag actccatttc atccaatcgc caagaaattt aataaagatc aatattttat
    78361 cgtaatcgat agtatgcgtt acaacgttat ctatgccgat tacgataatt tttatatcta
    78421 tttttatctt gaaagtgtgg gtgaatatgg tgattgataa ttctaaagaa aaagaacgtt
    78481 taaataagca aatttctgct atcaaaactt ccttagaaga gcattttggc ctcaaactct
    78541 ttcaagactc cgtaggcgag gatgagctac ctgatgattt taattacttc attctcgaaa
    78601 caggagaaat agaaatgatc actgagccaa aatatagcgt gggtcaaaat ctatatctaa
    78661 ctttctattc agaaaataga gaagatttaa caggagactc actagatatt atttcattga
    78721 ttcaaaatcg ttcgattcgt tttcagagaa tggatcccaa tcatttaaaa ctagagaacc
    78781 aagatcgcta tatcgatcaa ttggtattta cgtttagacg attattgaag agtgattgtc
    78841 atggctaaaa atagttggga gctaaaaata aatggacatg atgaacttct tgtgcggatg
    78901 gaacgctatt caagcgagag cgaacgactg attaacgaag cattgaaatc aaaaggttcg
    78961 gctattgcag tggataggat tacagaaaaa attcctgttt ctgaagcaga tttaagaaga
    79021 gggcaccaac acgcaaaaaa tagtcgtcca cttaagactc aatacattaa tttgggtttc
    79081 atcattagac ctacaagaaa atttgagtat ttaaaatatc ctgatttggg gataggtact
    79141 tctaaaagaa atcagccgga cgaatttatg agaagaggat taggtcttgc acttgatcca
    79201 attacagaac ttctgattcg tcaattcgat aaattaaata aataggggga acaacaatgg
    79261 ctaaaacaaa aactgtagta acaacgttcg ataacgtaag tatcaaacga attgctttta
    79321 attttaagaa cgcaggaaat gcaatcgcaa cgaattgtaa tggacaatta gatggcgaaa
    79381 cagaaatgca aacggtggtt aaaaaatgtg gagcgacaga agtaaaatca aaatctaaac
    79441 caatcaatat gacggtaaca attactgcac atgtaccgat ggaagtttat cgacgtttca
    79501 atgggttgaa acaagatgga cgtattaaac caggcattta ctcttacggt cctgattccg
    79561 taggcgaaga tttctcactt gctgcagaga tcgtggatga cttcgaagaa aatagaaagt
    79621 tagttggtat gttagcatgc acttcgaata caggattaac attctctatt gaaaatggtg
    79681 cggatgaagt agctgcgtta gaactagaaa caaaagttat gcaagatgaa tttggtaaat
    79741 tttatcatga agcaattgtt gcagaacttg aagaagactt aacagatcaa tggatgacga
    79801 atctatctgc tgatgtgatt aaaaaaactt cagttgtgac aactacggcc actcaatcac
    79861 agtaaaaaaa acggaggtag cgaaatgaac gaagattact caaaaattga actaaacgat
    79921 ggaacaattt tgaatttaga acctaaactg aatatcaaga aattattgat gatcaataga
    79981 gattttaaca cagacgagtt tgcaaaaatg actgtgggaa aaggatccat ggatatttct
    80041 gttattcaag gtgcaaaggc tgtgtatatt gcttaccgcc aagcgaacat gactgattat
    80101 atttcattcg atgaatttat cgataaatgg gattttgata tggctactgc cagctatatt
    80161 tatcaattga tgatgttcaa acaagcacgc gatgcttatc aaaaagaatt tgaaaaagca
    80221 aataaggaaa aaaagcttca aaagtaaaaa ttccaaagct cttagttgaa acgtgggtcg
    80281 atgtctattc gatgttgacc gacgtttttt ctatgccttc agatttggtt ttaagcgata
    80341 tctgtttaga tgacattttg caaatggctt acaacaagag tgcttatgaa ggatggaaaa
    80401 actatgcaat aaaccaatcc cagaaaaact aaagaaagga ggtaaaaaat ggctaaaaag
    80461 agaacagaag cagaagtaac tttcatagct aacgatgacg gattgaaatc tacgttaaaa
    80521 gaaatcagtg ctgaattaac taaaaataga gcagaattaa aactagaaca agctcaatta
    80581 caacagactg gttctgaatc agacaagtta ggaagtaaat tatcttcttt agaaaagcag
    80641 tatgaattac aaagtcaaaa agttgaagta actagccaac gtttagccaa tgcgaaaaaa
    80701 tattatggag aaaattccac cgaagttcag aaacttgaaa gagaactgat taatcaacaa
    80761 acagcgcaac aacgtttgtc aaacgaaatt gataaaacga gtaatgcact agctcaagca
    80821 aaaggcgaaa tacagacgta cgagtctaca atgcaacagt tggatagcga acaaaaaaat
    80881 gttcaagcta gtgcttctct gattgaatcc gaatacaaaa aatggcaagc aactgctggt
    80941 caatcagctt ctgaagccga gaaattagcg aaagcccaag aatatgtttc tcaacaatct
    81001 gaaaatgcgg agaaaacgat agatatccta agacgacagt tagaagctac acagtctgaa
    81061 tttggcgcta catccacaga agcaatgcag atggaagcga agcttaatga tgctgaacgt
    81121 gaatttgaag agttaggaca agctgctaaa aatgtagata caactaactt ggacgatatc
    81181 ggaagcaaaa tagatatgaa taatttaatg gaagcttctg acgttttaag cgacattggc
    81241 gataagctta cagaattagg gaaacaagca gtggactctg ctaacagtgt aggtagttcc
    81301 cagagtaaga tacaagctaa ttttggtttg tctaaacaag aggctgaaga attaacgaat
    81361 gtagccagag acatttatta taaaggtttt ggagaatcgt tagatcagtc cacagatgca
    81421 ttgattttgg taaagcgtaa tttaggagat ttaaataatc aagatttaca aaatatcacg
    81481 gaacaagcta tggtcctaga aaacaccatg ggcgctgata tggatgaaac gttacgtggt
    81541 gtaaatggct taatggtcaa tttcggcttg agtgctcaag atgcaatgga tttaatggtt
    81601 tcgggtactc aaaacggttt agataaaacg cacgaattag gcgacaatat ggcagaatat
    81661 agccaattat ggagtcaaat gggatattca gctgatgaaa cgttcggaat gcttcaaaat
    81721 ggtttagatg cgggtgctta taaccttgat aaagtcaatg acttagttaa ggaaatggga
    81781 atatcgttaa cagatggtcg atttgagcaa aacatggata tgtttagtga aagtactaga
    81841 aaagcttttg aagagtggaa aaatggcgga ggaacacaaa aagacgttat taattccatg
    81901 attcaagatt ttagcaatat ggatggtcaa tacgaccaat taaataaagc ttcgacaatt
    81961 tggtctgcgc ttggcgaaga taatgcgatg aaagttgtcc aatctttaac tgatgttaac
    82021 catacatttg atgatgttag tggatctgca caaaaaatga atgaagattc tactactccg
    82081 ttgcaagagt tgaacgggaa aatagctgaa ttaaaggatt cattagctcc tataggcaac
    82141 acaatcatag atgcactcga accagtaatt gattttctag gaaagatggc tgatgcgttt
    82201 aataatcttc cacaaccagt acaggatttc attgttgcta taggtggtct tacagcagca
    82261 tttggcatac tgttaccggc aatactagcc gtgtcatatt tatttggtcc gatgatgctt
    82321 ataattggag gaattatagc tgttatagca ggcgttattg tggcaattaa gaactggggt
    82381 gcaattactg actggtttag tggtttatgg aaaaaattta ctgattggtt gggtgatact
    82441 tgggaaagta taaaagacgg agcctcatca gtttgggatg gggttaaaga aacctggtct
    82501 ggatttgtag attgggttca agatatttgg caaggagttt ctgattggtt tggagagcta
    82561 tggagcggat tagttgaagg agcttccaac atctggcaag gagtccaaga aacttggcaa
    82621 acattcgttg attgggtttc aaatatttgg aacggagtca aagaagtatg gtcgattatt
    82681 tgggcagaca ttgtaggaat tgttcaaata ccatggacat taataacgtc attgattcaa
    82741 gctggtatta atattatcgt gggtattttt gatgtagctg gacagttatt gggcgcagct
    82801 tggcaagctg tttggacacc tatttctgat ttccttaaaa atacttggga tactatgaca
    82861 caatggataa gtatcgcttg gaatgggatt gtaactacat tccatactat atttgatcca
    82921 gtagtggcat ggtggaatgg tatatggaca gctattagta ctacggcttc aaatatttgg
    82981 aattcaatta gtgcaacagc ttctagtatt tggaacagta tcaagaatac aatcactagc
    83041 ttggtacaag cagctgctac agtaattcaa aatatttggt caactgtatc tagttggtta
    83101 ggtggaattt ggaattcaat cagctctaca gcatcaaata tctggaatag tgtgactagt
    83161 agtataagca atgctataaa cgcagctaaa agtgccattc aaagtgtttg gaatagtata
    83221 tcttcgtgga ttagcggaat ttggaacggt atcaaaaata ctgctttgaa tctttggaat
    83281 ggaattacaa gcactattag ctctaaagta aacgatggaa aaaatgcaat ttcaagcggt
    83341 tggtccaatc taacaggtat tgtttccgac atattcaata atgttaaaag tacaattgct
    83401 aacatatggg aaggcatcaa aaagactgtt agcgctccaa ttgattggat tagagacaaa
    83461 atcagtggca tttttgataa tttgaatatt tctataccac atattccgtt accggaattt
    83521 atcatggaag gcagctttaa cccgctaaaa ggtcaaatcc cccatttgcg tgtaaaatgg
    83581 catgctaaag gaggtatctt tactaaacca actttactag gtggaatgaa cggtgtcggt
    83641 gaagcaggac ctgaagcagt tttacctttg aaaagatctg ttttgcaaga aattggtgat
    83701 cgtatcttga gtagcacatc agtttcatct agggcacaaa cgattcaacc tgtgaataac
    83761 tacgaattca atttcacaat tgatggtaac gcagatgagg ttactatgaa gcaaacaact
    83821 caacaaatca ttgatagcat tacaaaaatt caaaatgata atgcttcggc atggcgttaa
    83881 acaggagagt atttctcctg tttttttagt attaaaaagg atgtgaaaaa atgactgatt
    83941 gtatacattc tataatcgat ggatttcctg attatttgca taaattggct ttagctgaaa
    84001 gaccaaccat accttctcca aaaagacaga gagttgaaac ttctgtttta ggtaggttag
    84061 gtggcttagt acaagattac tcgtttgaag atatgtcgtt tacattgcac tataactatt
    84121 tagaggatgt ggaagaccat caagcgttca agcaatcgtt ttatatcatg cgtcattggt
    84181 taaactatgc aaagaaatta gaattctctg acgatcccaa cgtctattac gttatccaga
    84241 ctatcgatat tggggatgca gaaaacgata ttgttgaatg gggagagttc gatgtaaata
    84301 ttactgcgaa accattcgca agagttcaag aagatgtacc tataaccgta gataaaccac
    84361 agtcatttaa cttgctgaat aatagtttag aagaaagttt tccaaagatt atcatcactc
    84421 cttcagctac ttcatgccag ttcatcttaa atgattatgt gtttagtttc gaaggcttag
    84481 tagcaggaac tgatgtagtc attgatagtg atttgatgct ttgctacgaa gagcaatcgg
    84541 acggagatat tttagatcgg tccaacaaaa tgaagaccat gcaatatccg acattgcaag
    84601 tggatatcaa tcattttaat tgcactggtt tgagcaaaat acaaatttat cgtaacgggt
    84661 taaggtaggt gaaatagatg atcgataatt taataactat ttacgataaa aatgacgcga
    84721 ataatttagc tgaacattta tatgatacgc aaggtttagg cgctttgtca gactggttaa
    84781 cagctactgt tagcaataaa ctaaacggag ccgagatatt tcagggtact tattcaataa
    84841 acggaactaa tgcagatttg attgtagacg gacgtattat tcagtgttat gtagatgaaa
    84901 atcgagcaaa acagcgtcta cggatttatt atgcaaaaac ttctgtaata ggcaatacga
    84961 tagaagtaaa agctgaacct attttcaatg atataagaaa atcggtgttg aataaatatg
    85021 acagcggaac agaaaagatc actgctactc aggcatggca aaacgcaaaa gttttagcga
    85081 aaccagctat cccttcgcag ttttctttct cgtcattagt agatacgctt gctaatgtga
    85141 agatagaaaa ggcgaatttt ttagaattct ttggtggaaa agagggatct attctagatc
    85201 gatttcatgg tgagtttcta aaagataata acacattacg tcatgaaaaa agtctaggta
    85261 cggatcataa aatcaaagcg atttatacta aaaacttaac tggtcttgac ttagagattg
    85321 atgctcaaag tgttttagtt ggagtttatc cattcattag cagctcttca gaaggagaag
    85381 acgagatcac tctaccagaa gaagttattt tcacggatta cgtggatgat tatcctgctg
    85441 gatatgtttc ttttgttgat tttaaagaca aagcgactga tgtagccaca ttaagggaag
    85501 ctgctaaaga ctggttgaaa acaaacatag ataaacaaaa accacaagtg agtggttcga
    85561 ttgaattagt accattgagg catcaaagag gctatgaaaa atttgttgat ctagaaaaag
    85621 tttcgatggg tgacggagta gatgtgtatc atccacagtt aaaagtgaat atgtcagcga
    85681 gaattgtgga atatacgttt aatgttctaa ccaattcata cgataaatta gttgtaggaa
    85741 acgtcaaaac aaacttctta gaaaacacag agaataatgt cagcaatttg attaatgatg
    85801 ccattgatca attgaaaaac ggtggcgaaa tcagtgattt actcaatgat attgtagatc
    85861 atcaaactga tatgattact ggtcaaaatg gtggttatgt tttattagat cctaaagaag
    85921 cgcctagtcg tattttgatt atggacacac cagataagaa taccgcaagg aatgttttac
    85981 aaatcaacaa tgctggtatt ggtttttcta aaactggcat taatggaaca tatgaaacgg
    86041 catggacgtt agatggcgga ttcaatgcct cgtttattac ggctggtgaa atagtaggaa
    86101 ttactattag aggtactaca ttaattagtg atggtgctga ttatagaaca agtattgcta
    86161 atggcaaaat gacttggtac tcaaaaaaag ttaacaaaga tattatggag ctagaagcac
    86221 gtgattatgt aagtgctgat gccggtattg tatcatacac catgaaaact ggtggtggtt
    86281 tcatgattag aaatccacag ggtaacttgg tttttagtac gtgggataat ggtaataaca
    86341 gaccgtttct atcttttggt gcgcccaatt tcaggtatag caatgctagt tatgtaactt
    86401 ctggcgacgg tagttcttta agcattaatg gtagtgcggg taactcatgg gaatttaagg
    86461 tagctggtag gactatgaaa tttactagtg atggtatgct aacgttacca ggttgttttt
    86521 ttggttcatg ggaagatggg aaacttgcta ggtttgaaca atcaacggta caagtatata
    86581 aagattttac tgttagaggt actaaaaact caactgtacc aacagaacat tatggacaac
    86641 gactattgaa cgcttatgaa actccagaat attatttcgc tgattatggg gaagccgtta
    86701 caggtgacaa tggtaaagtt cgtgttgata ttgaccccat gtttgctgaa acagtaaatc
    86761 taagtcggta tatgacacat gtgacaccta cagaactagt tttgtgtgct gttactcatg
    86821 aagatattga ccatttcatc attgaaacta gtaagccaaa cgtattagtt agatggaatt
    86881 tagtggcaca ccgtctaggg tatgaagata ttagattaaa agaggataca gcatatgata
    86941 gcacagtgct tgaccaaaaa cgtttttaaa acgaagacaa ggaggtatat aaatggctag
    87001 cagtttatat aatttggctt tagatttcag caaagaatta aactacacca aagctattat
    87061 ggctcgtcaa ggtgataaag ggattacggt gacggttaaa ccgtttctaa atggcttgca
    87121 gatggatacg agtggcggaa catttacttt aaaaggaaca acaccatcta accgttacgt
    87181 agataatgtt gcaactagtg taactagtga agaagtcacg ttttctcttg atggcacatt
    87241 tatgagtgaa gcaggatatt ataaacactg ctacgtagaa tatagaaaag acaatcaaat
    87301 tttaacaacg caagatatca tttttttctc actaggagtg tctgacattt cgcaaggcca
    87361 agccgatgaa tatgtttcgc aattagaaga gttgattcga aagtacaacg aaacttttga
    87421 tgcttttatg gctgaaatca aaggtagagt ggatagctta aatcaacaga ttactgattt
    87481 aactggtcaa gctaaaacgc tacaagacaa gttagatgct ctgaaagaag aaatttctaa
    87541 gttaggtaac ttacaagtga tgtacagtaa cagcatcgac ttcggggact atgattattc
    87601 ggagaacccg aatttaatgc catatattac agaaccttgg gttggacctt tgttaggtaa
    87661 tggacataca gttaaagata gtgttaaaag ggttataact catactaaaa ctagaacagc
    87721 taattccggc gacatattat cgcttggctt aggtattccg tgtacagcgg aagctaataa
    87781 taggtatctt ataaccactt tacgaccatc tacgacatac acattaagcg ttactatgag
    87841 tgtcggttca gattggactg gagagaccaa tactataggg gttaggctta gatatttaaa
    87901 tgaacaaggt ggtatagaac tgcccattaa tgcattaata ccagcaaatg tagaacgcga
    87961 taaaatggtc actcatacat ttactggtat aacaaaagat aatgtaacta gcataacaaa
    88021 ctgttatgtt gaaatattct cacttaatag tgaatataaa ggaacggtta gtgtcagcta
    88081 tgacgttaag ttaaaagcac actaccctaa cttactagat ggcccttact ggttaggtaa
    88141 agttccttta ggcgagaata ttgctgaccc tacagttgtc tttccacaca aaactagtga
    88201 atatatggtt tatggtagac gcaatacaga aaactatatc gctgaccaaa catataccat
    88261 atcaatgaaa gcaactaaac taacagttca atcgtttgcc gtgtatattg ctgctggccg
    88321 cgttaaagta ggagacatga agcctactga ggggttagct aatacatggg agttaacttt
    88381 tactgtaact aagcaacata tagatagtgg ggtaactaat tatctggaaa tataccaata
    88441 cccgtcagct acaaaaggag ctgttcaaat tgagtggctc aaactagaaa aaggcaatac
    88501 tcgaaccccg aatattagtg agtataaata tcgtggtact ggtatgcgtg attcaaacaa
    88561 tccaaaagat tatgtttggg atctagcacc agaatatgtc gaagataatt tggccacaga
    88621 tattaaaatt tctgaaatta ctggtaaagc aaacaattat accgatggga aagtatcgga
    88681 gattaattcg cagttgactg cttcaattaa tgaagtagac accacagcta aggatgctca
    88741 aacaaaagcg aatgctaatg cgactgctat agatgaatta gacaataaga tcgatgaacg
    88801 cattaatgat acagctacta ccacattaac agttacaaac gggaataccg gatcagcaaa
    88861 gctttatcgt gaaggaaaaa cagtttctat atattttgtg gctttaaacg gaaaaagcag
    88921 tggtggaaat gattcaacga tactaacaat tccagaaggc tatcggccac caattagttt
    88981 tgagcaactg gttggctcga tagaccgttc tactttgaac agtgctcagt tatctattgg
    89041 tgcagatgga gccattaaat ggcgaagaaa ctcaagttat ggatcggatt atacctttgc
    89101 aattacttac acgatttaga aaagcgtgaa tcgatatgaa ggcagcatat agaccaattg
    89161 aaccttacgg attcgagcaa atcattgtga atgatgaaga acatttaccg gaagaatgca
    89221 cagaagtcga accaccgatt ccaaattgga aaccgaaatt caattactgg gagggaaata
    89281 aatgaaaaac atttggaaat atggacgtac tggcggagag tacgcaggaa aagtattgga
    89341 cgacatgctt gtatccgttc cttacacaga tcagccaccg cttgaaggaa ttcgtgctga
    89401 tggcgaaccg ttaacaatcg ctgatcaaat gtttgatcct aaattgaatc aatggattat
    89461 tttagcgaac gcactagatc acaacgattt aaacaatctc aaagcaatgt atgagtcgtt
    89521 agaaaatgag aacggcgatt taaaacagat caatgccaaa ctcatgctaa gcgatgtagc
    89581 aattaaacag gaaaatactg cattgaaaga aaaagcggat agtttagcac aaatcaattc
    89641 aaaaatgatg cttgcttcgt tacaaaatag caaagacatt tcagaaatta aagagcaact
    89701 aaatccagct tcaaagggag gtgagtagta tgtttagttt tagcgatgtg aaaatgatgt
    89761 atgattgggg ctgtttcact aacgaacaag taatggtttt cgttccgttg tgcattactg
    89821 aagaaaaagc agataaaatc attagcaaag aagagagcgc atcttaattg atgtgctttt
    89881 tattttgatt caaggagttg tcacatgatt aatttagggg aatggggagc gatagcagga
    89941 tcaataaccg ctatcgtttc tttgatttta ttagtaataa aaccaattac tgcatctttc
    90001 tcgaagatta ctgagactct ttcaaaagta agtcacaatt tagatttgct gactaaagat
    90061 ttagaatcga gcaaatcaga tcgattgatg attcatgaag aactaaagaa acacgatgaa
    90121 agattagata cacatgcaga aaaattggta gaacacacgc aacaaattaa aactttattt
    90181 agggagagaa gaaaatgaat aataaaacgt tcgaagtact aaaatggttc gcactggtaa
    90241 ttattcccgc actagctact ttcgtggggt tagttggtaa agcgctcaat tggcagtaca
    90301 cagatatctg tgttgtcatc attactggtt ttggcgcgtt tttagggagt gtgttgggtg
    90361 tatcaaatcg aacctacaaa atgttctcgg ctgaaagcga agaaggagga aacaaatgaa
    90421 aaagaaaatt actattactg cgatgagcct gttaacggct ctttttttat tacccattaa
    90481 tacgtttgct tatactatta atgacgagta taatttagcg ccgaatcaag gagactccag
    90541 attagcaatt cctaacaaaa ttattttgca cgaaactgga atagatgcac cagcaagaaa
    90601 cgtagccgcc aacatgaaaa ataattataa cggaagcaat tcttatacta cagatgttat
    90661 tggtgacggt gggattgttt accgtgtggg tgagcaagga tatgtttcgt ggggagctgg
    90721 taacgctaat ccttatgcgc ctgtacagat tgaattacag cgcacatatg ataaagcatt
    90781 gtttgaaaaa aactatcgag cttatattga atatacaaga gatagtgcaa aaaaatatgg
    90841 aattccattg actcttgatc aaggaacttc tttatttaca aaaggaatca tttctcattt
    90901 gtgggtgaca aattatgttt gggggaacca cacagatcca tatggttact tatcgcaaat
    90961 gggagttagc aaagaaaagc ttgcttatga tttagctcat ggatttaccg atgaaaatcc
    91021 aacaacttct gaaaacaagc ctgtcattga tccaacacga gctggtgcag ctaatcctac
    91081 gctgacagat ggaacaaatc acgcccacat tgatcagttt ggggaaatcg aaaacgcaaa
    91141 cttgcatgtg gctggatggc acattgctaa ctataaatac gagtatattt tcattatgga
    91201 ctacaatact gggaaagaat tagctcgagt aagagctgat ggaatttata gaccagatgt
    91261 aaaccaagct tataatactt ctggaaatgt tggttatcat gtatctttca atatgcgtaa
    91321 ttttcctagt aagaaagtct atgtaatgat gcgggcaacg aatgatccag agggaaacac
    91381 taaaggcggt gcgcaagatt tccatgacaa acgttggtat ttaaatattc ctaaacgata
    91441 aaaatagctc ctcgttgagg agcagtacat aactatattg acaactataa aaattattcg
    91501 ataaaatagt gatgttatcg catatcttca ctatcaccca taaatagtca cactccaagc
    91561 tat
//
LOCUS       DAMEET010000002        63868 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.002, whole genome shotgun sequence.
ACCESSION   DAMEET010000002 DAMEET010000000
VERSION     DAMEET010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 63868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.002"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(11..616)
                     /gene="dhaL"
                     /locus_tag="PB780_000117"
     CDS             complement(11..616)
                     /gene="dhaL"
                     /locus_tag="PB780_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HDE0732203.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(644..1654)
                     /gene="dhaK"
                     /locus_tag="PB780_000118"
     CDS             complement(644..1654)
                     /gene="dhaK"
                     /locus_tag="PB780_000118"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDE0732204.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(1629..2075)
                     /gene="dhaM"
                     /locus_tag="PB780_000119"
     CDS             complement(1629..2075)
                     /gene="dhaM"
                     /locus_tag="PB780_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HDE0732205.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            2178..2498
                     /locus_tag="PB780_000120"
     CDS             2178..2498
                     /locus_tag="PB780_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDE0732206.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(2610..3479)
                     /locus_tag="PB780_000121"
     CDS             complement(2610..3479)
                     /locus_tag="PB780_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDE0732207.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(3575..4879)
                     /locus_tag="PB780_000122"
     CDS             complement(3575..4879)
                     /locus_tag="PB780_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDE0732208.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(4876..5385)
                     /locus_tag="PB780_000123"
     CDS             complement(4876..5385)
                     /locus_tag="PB780_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732209.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(5560..7695)
                     /locus_tag="PB780_000124"
     CDS             complement(5560..7695)
                     /locus_tag="PB780_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDE0732210.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(7778..9433)
                     /locus_tag="PB780_000125"
     CDS             complement(7778..9433)
                     /locus_tag="PB780_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDE0732211.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(9435..9749)
                     /locus_tag="PB780_000126"
     CDS             complement(9435..9749)
                     /locus_tag="PB780_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDE0732212.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            9919..10179
                     /locus_tag="PB780_000127"
     CDS             9919..10179
                     /locus_tag="PB780_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HDE0732213.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            10193..10507
                     /locus_tag="PB780_000128"
     CDS             10193..10507
                     /locus_tag="PB780_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HDE0732214.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(11237..11791)
                     /locus_tag="PB780_000129"
     CDS             complement(11237..11791)
                     /locus_tag="PB780_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDE0732215.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(11917..13846)
                     /locus_tag="PB780_000130"
                     /pseudo
     CDS             complement(11917..13846)
                     /locus_tag="PB780_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(14152..14868)
                     /locus_tag="PB780_000131"
     CDS             complement(14152..14868)
                     /locus_tag="PB780_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDE0732216.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(14869..16692)
                     /gene="glpO"
                     /locus_tag="PB780_000132"
     CDS             complement(14869..16692)
                     /gene="glpO"
                     /locus_tag="PB780_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HDE0732217.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(16706..18202)
                     /gene="glpK"
                     /locus_tag="PB780_000133"
     CDS             complement(16706..18202)
                     /gene="glpK"
                     /locus_tag="PB780_000133"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDE0732218.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(18338..19798)
                     /locus_tag="PB780_000134"
     CDS             complement(18338..19798)
                     /locus_tag="PB780_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDE0732219.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(19788..21083)
                     /locus_tag="PB780_000135"
     CDS             complement(19788..21083)
                     /locus_tag="PB780_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDE0732220.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(21415..22092)
                     /locus_tag="PB780_000136"
     CDS             complement(21415..22092)
                     /locus_tag="PB780_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HDE0732221.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(22122..23213)
                     /locus_tag="PB780_000137"
     CDS             complement(22122..23213)
                     /locus_tag="PB780_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDE0732222.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(23314..23748)
                     /locus_tag="PB780_000138"
     CDS             complement(23314..23748)
                     /locus_tag="PB780_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDE0732223.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(24332..25789)
                     /gene="ascB"
                     /locus_tag="PB780_000139"
     CDS             complement(24332..25789)
                     /gene="ascB"
                     /locus_tag="PB780_000139"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDE0732224.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(25786..27681)
                     /locus_tag="PB780_000140"
     CDS             complement(25786..27681)
                     /locus_tag="PB780_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDE0732225.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(27739..29514)
                     /locus_tag="PB780_000141"
     CDS             complement(27739..29514)
                     /locus_tag="PB780_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDE0732226.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(29564..31060)
                     /locus_tag="PB780_000142"
     CDS             complement(29564..31060)
                     /locus_tag="PB780_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDE0732227.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(31254..32876)
                     /locus_tag="PB780_000143"
     CDS             complement(31254..32876)
                     /locus_tag="PB780_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDE0732228.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(33204..34517)
                     /locus_tag="PB780_000144"
     CDS             complement(33204..34517)
                     /locus_tag="PB780_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDE0732229.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCILMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(34798..34986)
                     /locus_tag="PB780_000145"
     CDS             complement(34798..34986)
                     /locus_tag="PB780_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732230.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKE"
     gene            complement(35006..35428)
                     /locus_tag="PB780_000146"
     CDS             complement(35006..35428)
                     /locus_tag="PB780_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HDE0732231.1"
                     /translation="MESVYRQERKSNRKKGQTMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGVLTFNFSDPEGNYFVIGKAESSD"
     gene            complement(35573..36283)
                     /locus_tag="PB780_000147"
     CDS             complement(35573..36283)
                     /locus_tag="PB780_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732232.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(36301..36798)
                     /locus_tag="PB780_000148"
     CDS             complement(36301..36798)
                     /locus_tag="PB780_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDE0732233.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPKHLADYLTQAYAPEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(36835..37287)
                     /locus_tag="PB780_000149"
     CDS             complement(36835..37287)
                     /locus_tag="PB780_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDE0732234.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(37422..38027)
                     /locus_tag="PB780_000150"
     CDS             complement(37422..38027)
                     /locus_tag="PB780_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDE0732235.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(38582..39217)
                     /locus_tag="PB780_000151"
     CDS             complement(38582..39217)
                     /locus_tag="PB780_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDE0732236.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKKSMTISNKEMA"
     gene            complement(39219..40088)
                     /locus_tag="PB780_000152"
     CDS             complement(39219..40088)
                     /locus_tag="PB780_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HDE0732237.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYISMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(40111..40518)
                     /locus_tag="PB780_000153"
     CDS             complement(40111..40518)
                     /locus_tag="PB780_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDE0732238.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDRHKLAELYEQVGLKFSELYENDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRSD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(40537..41709)
                     /locus_tag="PB780_000154"
     CDS             complement(40537..41709)
                     /locus_tag="PB780_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDE0732239.1"
                     /translation="MSVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAILWMATMTTVFGY
                     FAGLRGIVLILVGGFIGACFAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVA
                     KLFGEKGENKKSIEDIKLPTHFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGD
                     QNYIMFAFLQAIQFVVGVYVLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFP
                     YSPNAVILGFITTTIGTIIAMFTLPMFGLAMILSGMLTNFFAGGTAGIFGNAVGGRRG
                     AIIGGIAHGFFITLLPALLVTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(41736..42038)
                     /locus_tag="PB780_000155"
     CDS             complement(41736..42038)
                     /locus_tag="PB780_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDE0732240.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(42063..44149)
                     /locus_tag="PB780_000156"
                     /pseudo
     CDS             complement(42063..44149)
                     /locus_tag="PB780_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
     gene            complement(44638..45403)
                     /locus_tag="PB780_000157"
                     /pseudo
     CDS             complement(44638..45403)
                     /locus_tag="PB780_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
     gene            complement(45514..46383)
                     /gene="mdcB"
                     /locus_tag="PB780_000158"
                     /pseudo
     CDS             complement(45514..46383)
                     /gene="mdcB"
                     /locus_tag="PB780_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
     gene            complement(46408..47492)
                     /locus_tag="PB780_000159"
                     /pseudo
     CDS             complement(46408..47492)
                     /locus_tag="PB780_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
     gene            complement(47516..48109)
                     /locus_tag="PB780_000160"
     CDS             complement(47516..48109)
                     /locus_tag="PB780_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HDE0732241.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWATEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(48113..49789)
                     /gene="mdcD"
                     /locus_tag="PB780_000161"
     CDS             complement(48113..49789)
                     /gene="mdcD"
                     /locus_tag="PB780_000161"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HDE0732242.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDFIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEETIVISIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKFRQAGYPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(49779..50078)
                     /locus_tag="PB780_000162"
     CDS             complement(49779..50078)
                     /locus_tag="PB780_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HDE0732243.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(50180..51826)
                     /gene="mdcA"
                     /locus_tag="PB780_000163"
     CDS             complement(50180..51826)
                     /gene="mdcA"
                     /locus_tag="PB780_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HDE0732244.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVVADKADKDGNL
                     YTGFNTEETPTIVEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADEPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALVVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLREAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(51833..52744)
                     /locus_tag="PB780_000164"
     CDS             complement(51833..52744)
                     /locus_tag="PB780_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDE0732245.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLTFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEMVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            52864..53154
                     /locus_tag="PB780_000165"
     CDS             52864..53154
                     /locus_tag="PB780_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDE0732246.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLYTLEEGINYCLRSMRRSLLPSYCI"
     gene            53106..53720
                     /locus_tag="PB780_000166"
     CDS             53106..53720
                     /locus_tag="PB780_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HDE0732247.1"
                     /translation="MSSVNEKIVTSVILYLEVASVSIPNLVRIFMEKHPDIQLTIMQH
                     HLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAIPKKHPLAKKEHLQLEDIQRTPIL
                     MLSKSNAFRHTLDAALEAKSIELTISSTTDDPATLRSIVKQGLAVSFFPKVSWSYDKN
                     DPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAKTMREFYLGK"
     gene            complement(53907..54269)
                     /locus_tag="PB780_000167"
     CDS             complement(53907..54269)
                     /locus_tag="PB780_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDE0732248.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            54615..56052
                     /locus_tag="PB780_000168"
                     /pseudo
     CDS             54615..56052
                     /locus_tag="PB780_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(56059..58611)
                     /locus_tag="PB780_000169"
                     /pseudo
     CDS             complement(56059..58611)
                     /locus_tag="PB780_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
     gene            complement(58725..60056)
                     /locus_tag="PB780_000170"
     CDS             complement(58725..60056)
                     /locus_tag="PB780_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDE0732249.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYSLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELAAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQMIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCTHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(60022..61500)
                     /locus_tag="PB780_000171"
     CDS             complement(60022..61500)
                     /locus_tag="PB780_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDE0732250.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPKKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLKNPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(61805..63640)
                     /gene="tdc"
                     /locus_tag="PB780_000172"
     CDS             complement(61805..63640)
                     /gene="tdc"
                     /locus_tag="PB780_000172"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HDE0732251.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     EQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTTHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHMTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKEIAKAMEKKLNEIIQ"
BASE COUNT        18227 a        13693 c        10803 g        21145 t
ORIGIN      
        1 tttattgaac ttaaggttga ttgatcacat ctaaaagcgt tgaaaataaa atggcactag
       61 aagcagctcc tgggtcgata tgtccgattg cacgctctcc tagataggaa gctcgtcctt
      121 ttttagctac caagactttg gtgtgttctt ttgcctggtc aatgatttct tttgttaaat
      181 tccctgcctt caatgcctct gtgactggca gccaaacatc taacatcgtt ttatcgtcag
      241 cttctgcctt tcctcggctt tgaatacccg ctgttccctg ctcgatcact tcgcctaatt
      301 tttcttgaga atcaatagaa tcgacctctt ttgtcgcttt catcatgttc atgaatgcag
      361 atccgtataa cggaccagaa gccccaccga ctttcgatat catcgccata gagaatactt
      421 tgaatgtatc gctgattgtt tcaggatgat cggcttgaaa agcttcttca taagctgaaa
      481 cccctcttcc catgttattt ccatggtctc catcgccaat cggggtatct agatcactga
      541 gatattgttt gttctgattg attgcctctg caaattgcgt gagccattct tggatttctt
      601 gaacggttag tttcatatta tcaccctttt atcttgagat tttttaccat gaaatcgtct
      661 gtacattgct ttctaaagaa tctttccatt gtgtctccgt caagtcgatc agtgttagag
      721 aaagaccttg catatctagt gaggtcataa aattaccgac ttttcggaaa actacattga
      781 cgcctctttc ttccaacaga gctaaaacat cattcatgaa cacgaactgt tccatcaaag
      841 gcgtcccgcc cattccgttg actagtacac ctgcttcttt tggtaaattc gagtaatcat
      901 ctaaaatctt agtaacaagt tcctttgcca gtactttaga aggctgcatc ttttctcgac
      961 gatagcctgg ctcaccgtgg ataccaacac catactcgat ctcatcttct cctagttcga
     1021 atcctggttt ccctacttct ggtacagttg ctgctcgaag cgctagaccg atcgtcttag
     1081 ttgcctgtac gaccttttct cccagttctt tcaactcagc tagggaagca ccgtttcttg
     1141 ctgcatcgcc cacgattttg tgtacaagga cagtacctgc aactccgcgt tttccagcag
     1201 tatacgtact attttcaacg gcgatatcat catctacgat cacactttct acttcaatat
     1261 cgtccatagc agctaattcc tttgccatat cgaagttcaa tgcatcccct gtataattct
     1321 taacgatcag caaaactcct tttccactat cggcttcctt aatagccgtc tgaatctgat
     1381 ctggagttgg agaagtaaac acatcaccaa gcaccgctgc acttaacatc ccttccccta
     1441 caaacccggc atgggatggt tcatgtccgc ttcctccacc agaaaccaaa ccaacttgtt
     1501 ccgctttctg attcttggct acaacacggg aagattctac acgatgaaca agttctggat
     1561 agctttttac taatccttcc agcatttctt cgacaatatt gcttggatca ttcatgattt
     1621 ttttcaattt attcccatcc tttctttccg gcattttgtg cttctgttaa gatttgctga
     1681 gtatcatctg ttataccggc agtgatggct gctgcaaaag ctccttccac taaaggcgca
     1741 tccaccagat gataacgttg ctgctgctct tcttccaaca tgtcttttgc cagttccgag
     1801 ttcaataccg cgctaccgat atccgcaaaa acaaaataac tagctgcatc gcttgattca
     1861 ttgactgctt ctacgatttt catcgggtct gatccgatct cgccatttat tccgccgaga
     1921 gaaaaaattt gcacctcttc ggatttttgc atctgctcaa tcatttcttt aataccatct
     1981 gttacttttt gactatgaga aataagcaaa atactttttt tcacaagaat cttcctttct
     2041 tttacatagt tgctaacgat tggtttcgct ttcattaatg aacaattcaa gtatataata
     2101 aatttctaat tttgggaaaa tatgtgtagt gcttcttgaa gtttgttata ctttaattaa
     2161 aagagaggag tgatcgaatg attacaacaa caacgaacag tgtagaggga aaagccgtag
     2221 tagcttataa agggatcgtt tttggtgagg tcattactgg gatccatgca tttaaagact
     2281 tggaggcggg gttgcgaaat atctttggcg gccgctcaaa aagctatgaa aacgaactaa
     2341 tgggtgcaag agaagaagca cttgccgaaa tggccgaacg tgcaaaagca ttaggagctg
     2401 atgcgatcat tggcgtaaaa atggactatg aagtattagg cagcgacaat ggcatgctga
     2461 tggtcacatg tagtggaaca gctgtctcca cgagataagt aggggacatc tgttttaata
     2521 actaaagaga gatgggacat gtagtggcac tcaagaatta gatttttcag tctaacttcg
     2581 aggtactcta ccttcccatc ctctcttttt tatttctttt tgcggatgat tccgtaagct
     2641 gctgcgacta acacacccgc ccctgctaag gaataactga cagaagctgt tttttcattt
     2701 gtttttggca aagtctgact tgttgatttg gatggctctg tcgtttgctg aactgtttct
     2761 gtcgtgcttc cagaggtcac tgatttttct cctgccgaat gcaaaatatc ttttattcgt
     2821 tggaaactag ctgctaagtc agcttttttt gcttcaaaat cattagcaac tgtttgtgct
     2881 tcgctgatct ttccgtggat tgcttgactg gccgctgtga tatccgcttg agtttgactt
     2941 agctttccag aagctgtttg agcaacctga tttaattgct gattgaattc ttttgcttgt
     3001 tggatcttcc cgtcaagatc tgtttggaca gcttccgctt ttgctttcgc ttcggcaagc
     3061 tttttctcaa gttgtgcacg gtcatttccc actttatttt taagatcagc taaatcttga
     3121 tcgattgctg cttggtcttc tttcaatttg ttttttgcag cttctaatgc ttcttcgagt
     3181 tcttttcttt tattttccaa gtcttgcaaa tgatcgattt ggtcagaaga ggtagaacta
     3241 tttattgatg tatcttctga agatgaaatc gtttctcctg aattatctgt cggtacattg
     3301 gaagtcattt ctgtttccga ggcaccgttt tcagcaccag aagagcttat ggttgttgaa
     3361 ggttctgttt gagcaggttc agaagtttcc gcaaaagctg tctgtcccaa taagaaaata
     3421 ctggaaacag caattggcca gattaacggc ttcattcttt ttttcatcac ttttcccatc
     3481 cttactactt tttaactaat ttcttattat aagcaaaaat gtttggaaga accataaaca
     3541 caacaggctg tttgtctaaa aaacggtgca tcttttattt ttttgtacgt tccgggcttt
     3601 tccatgtccc gcttccataa cctaaaatcg ttttgactgc tggaatgaaa gtcgtcacga
     3661 tcgttatcgc gttcactatc cagaacaaaa gcatatatag tggtgcgaaa agaaggtatt
     3721 tcaattttct tcgtcgatca tctacaatca gcgaagctag caactggaaa aatcctgcga
     3781 ccatttcaaa gcagatgaac acaaaactga atgtaaaagt aatataaatc tgttcgtagt
     3841 ttccttgata tacataatgc ccgattaatc cagcaaaaag aactacggaa atacaaaaga
     3901 atattgacca gacaatactc aatgtttgat caatgaagat gatcgttctt ccaatatgtt
     3961 taaaaggatg gagcatcact tttttgaaat tcgtcagcca gacttctgtt ccaccttttg
     4021 cccaacgttt tctgtgtcga tatagcatct tcaacgtcac aggtacttcc atgaaaaaga
     4081 taatctcagg agcaaaaaca gataaccagt catttaactg atgatcccaa gcaatactga
     4141 tgtcttctgt cgctcgatct tgtctgaatc cgcccacatc gatcaaggct tcttttcgat
     4201 acatcgtatt tgctccactg tacgcataaa gaccaccgaa aacagctgac tgtgttcgtt
     4261 taatgatgcc cacgatactt gagaattcaa ccgtttgaga ttttgcaatc aatttggtac
     4321 gattttggac atccatattt gcagtgaccg cagcaatatg tcttgcacca gggcgaatga
     4381 aataattcac atacctaatc agtgcatctg gctccggtac cgtgtctgca tcattgctta
     4441 aaatcaattt cccttttgca aaggcaagtc cgatattaaa cgcgtgggct tttcctttat
     4501 ttttttcgat ccgaaccaca cgaagattag cgtatttttt catcagtcta gctaatattt
     4561 ctggtgtttg gtccgttgaa ccatcgtctg tcactaatac ctcatagttg tgataattca
     4621 atttcgtcat caaatactcg attgtatatt cgatcacaat ttcttcatta tgggctggca
     4681 ccatgatcgt gatgaacggt tcgacactca agggaacatc tacccattct ttttgcttat
     4741 gtttgtataa aaacacataa caccagacac caataaacca tacaaatccg cctaaaatcg
     4801 gataagaaac gagaaaaaaa tacataatag acaaacaagt atctaagaat aaattacctg
     4861 tatgggtgat tgagatcatt tcagttccac cccattttct ttatacaaat ctgcaatgaa
     4921 tgtcgtatct agatttttct cttcggagac agaataaaaa cggaccgttt ctctctcttc
     4981 ttttgttcca aaacgttccg tatacacttc ttcaagtaat tcttgtcttt ttttcaattt
     5041 ttcatcttca tattcaaaag atttatttac ttttttgtat agatttcgat tgttccacct
     5101 agtcaataca aatgaggtaa tcaggaaact gaaaaaaatc aatacaaaaa agccgattaa
     5161 gaaatacagc attttctttt ctgataaaaa agcgtacaag gaaaaatcct tggctctttc
     5221 tggtctcgtt aatgggaaaa agacccatat aaaaggaaga acaacgatca tccatcctaa
     5281 aagagccact aacgtttgcc aaatctttaa ccagtaatta ccttttgaaa aatacttatc
     5341 tttcaccaaa attgatgcaa cttcttctct tgttactttt ttcatatcct cacctcacgt
     5401 ttttttgtta cgacgtagga gtttaccata catttcattt cttttcacat aaagtgttcc
     5461 taaatttttc ctaagaaaaa gaacaaaaca tttctcatca gcaaatgaaa aatattttgt
     5521 tctttttatc agttgttctt tttatttttt atctcttaat tatgttgctc tttccaatac
     5581 gacaatgctt ttcattcctc tagatatcgc cgtgtacatt tgtctgcgtc ttctttgctc
     5641 atctccatcg ttttcaaatg gatataagat tacatgatca aattccagtc cttttgctag
     5701 actcaaagga atgatttggc agttttctaa tccatatgaa ttttttttaa tcgattcttg
     5761 tagcgtttgg gtttcctctt ctgtttgggt gataactgcc gctttttctc cttttagaga
     5821 aaccaaaatc tcagctagta ttttcaacaa ttcttcagag gatcgaaaag agcggtattc
     5881 gggttctttt ccttgcggtc ggatcgatac gatgtcgatc ttctcttgat ccacagcata
     5941 ggttttgaaa agctctgtga ttgctccgct agaacggtaa ctatttctaa gatcataccg
     6001 atggatcggc agatggtagt cgtcgaaaca acgcataata tcagcaaaag tagtggaaga
     6061 attaaagatt gcctgattct cgtcacctac taaagtaaag tgagtttttg gaaataattc
     6121 gatcaataga cctaattgtg ccagggtata atcttgtact tcatcaatca atagatagcg
     6181 aaatgctggt acatttacct tttcaacaag taaatgacga atagctaata taatgactgc
     6241 ttcatcaaca gtacgtgtgg gttgccaagc ataagctgag ccatatcgtt tttcataaat
     6301 tctttcaaac aactgatgct catccaccca tgccatttct tcgatttgtc tggttatttt
     6361 tcgatatttt ttttgtaaaa gtttccttgc ataagctaca atactttgtt ccgaatcatc
     6421 agaaatcaat ttaccgaata attccaactg cctatcttct gtcaacgaga gtacctgatt
     6481 ctgcacagca ggactttttg cttgtttcaa aagatgattc ttccattcgc tcatcagtgc
     6541 ctttttcgtt gcttgaagcc tatcgatcaa tttaggatga gcaggaactt tctcatatat
     6601 tttttcgatc gtttttttag aaaggactgt ttttccattt ttagtaagtc cacggataaa
     6661 attcggatga tctaaaaaca aaggatctga ttgttttaga ttatcgataa actttttgct
     6721 acgcaaacgt tctgtctgct ccgatacatt ttctcccgtg atacgtttga aataagcctc
     6781 ttcaccttct atttcctctg ctgaaagctg tgaaaccagc tgtatgatgg ttagattcag
     6841 tggatttctt tcacctaatg atggtaggac attcgaaata tagtcgataa accgatgatt
     6901 aggtgataga atcagcaagt catcggatgt catcgtatct cgatataaat agagcagata
     6961 cgcaatacgc tgcatgatag cagaggtttt accacttcct gctacgccat ttaccaagat
     7021 attttgactt ttcacatccc gaatgattgt gttttgttcc gattggatcg tagcagtgat
     7081 agccttcatt tcattggtct catcttggcc taagacttct aataatacgt catcttgaat
     7141 agctacagac gtatcaaaaa acttgaccaa acgatctctt tccaaaagaa attgtcgtct
     7201 ttctttaata accgtatcga ttttattttg acgaaccgta taagaagatg gaccaatcat
     7261 gttattgtaa aaaagttcgg caattggtga acgccaatca taaacaagat tttcatctgc
     7321 atcatctgtg aaatttgctg taccgatata aaaggattct tttggctcat tatccagaaa
     7381 gtctacaacg acttttccaa agtagggact tttcaataaa cgttcgatct ttgctagctg
     7441 attttctgcg tttttctgtt tgatattcat ctgatcgatt tctctatttt tcatttcaat
     7501 catcgaaaaa gtatccaggt tatctaagta actgtcaaaa ttcaatcgaa gctcgccagc
     7561 catttctttc aacgactggt ttccttctat ttttgccgta tccatcgatt cccgcagttc
     7621 gtttttttta tcaattagct tttgatacac gttcttcaga tatgcttctt ctaactgttt
     7681 ttgtgtgttg accatacaca ttctcctctt tttttaaagt ggtggaaccg attagtttac
     7741 ctcttttaag agtcgaatgc aagcttttta acgagtatca tttgttagga tatatcagtt
     7801 cttcaggtcg gacagtttga tataaagaaa atgcaccatc aaggtttttt acgtggtagc
     7861 cagactgctt caagattctt tccgctagat agctacgtaa tccgctatga cagctgacga
     7921 tatattcttg ttggctgtct aattcattta atcggtcccg aagttcattc aaagggatat
     7981 gtttggcttc cttgaatttg cccttttcca attcttctgc tgttcgcaca tctaataaaa
     8041 tttttccttt tgctagttct gtcggcaatt catgccattg tacggtttca ctaagccctt
     8101 ctgcaatgtt cattgctgca tatcctagca tgtttactgg atctttggct gagccaaatg
     8161 gcggtgcata cgtcaattct aattccggta aatcgaagat cgtcaaatgc cctttgattg
     8221 ctgttgctaa aatatcgatc cgtttatcca caccttttgc cccaacacct tgtgcaccat
     8281 aaatctcgcc agtctctgga tggaagatca acttcagtaa aatatctgtt gcttctggat
     8341 aatagcttgc atgatctttt cctgatacat gaatgacttc aaaagacagt tcgttttctt
     8401 tggctgttct ttcacttaaa ccggtggatg cggcagtcat tccaaacgtt cgtataattg
     8461 ctgttccaat accgccttga tttcttcgag aaattccgga aatcgtgtcc gcaacttgtc
     8521 taccttgacg attggctggt gaagcaagag aaataagtgc atcttttcca gtgatttgct
     8581 gttttacaac gatagcatcc cccaccgcat aaatatcagg aatattcgtt tgataacgtt
     8641 cgtctactag gatacccccg cgtatgccta attcgattcc agcggtcact gctagctggt
     8701 tatcaggacg aacaccaatg gataaaataa ctaaatcagc agggatggta cgcccatcat
     8761 ccagtcgcaa tcgtttcccg tggtcttcaa atccagcgac agcatgagaa gtaatcactt
     8821 ctactccttg atgagacaat tctttttcta taaaagctgc catttcttca tccagtgttg
     8881 gaagaatatg tggtgcttgt tcgatcacca tgacttctaa acctcttctt tttaggtttt
     8941 ctgccatttc caatccgatg aatcctgcac caacgatcac ggcacgcttt ggctgttttt
     9001 ctaatgcatg catcacttta tcgatatctg gtacatttct aatagaaaaa acattgtcgg
     9061 cttctgttat ccctggaatc gatggaacaa ctggttttgc accaggagat aaaaccaatg
     9121 catcgtactg ttcaatgtgt ttttgaccct catgtacgac ttctaccgtt ttgttttcgg
     9181 gaaagatggc tgtcacttca tgatttggac gcacatctaa acaaaaacga gcggataatg
     9241 attctggcgt ttggacaaga agattttccc gttcactgat ttctcccgaa agataataag
     9301 gcaagccgca atttgcaaaa gaaacatacg gtcctttttc aaaaacaacg atttctgcat
     9361 cttccattaa tcggcgaagc cgtgtagcag cagacattcc acctgctaca cctccgacaa
     9421 taacgatttt catcttattt ccctcctact gttcgatcaa accattgatt catgccacca
     9481 cgtacattca ctacatcata accagatttc tttaattctt tagctgcttg tttactcctc
     9541 atccctgatt gacaaatcac atagacggtc ttttctttgt ctccatcata tctgtttata
     9601 ctttgtaatg gaacattttt agctcctttt atatgcccgc cccggtattc tgcaggtgtt
     9661 ctaacatcaa gtaacgctgg attttccaaa agcttttcct ttaattctgg gactgttatc
     9721 gattttattg attgaaatag caaattcatt ttctgcacct catttacatt tttataaata
     9781 cctcatgggg tatattaaac aggagcttta ttaatatgtc aaccagaatg actcttcatt
     9841 tttttcacta ctttttatat ttgcataaaa taccgataag gggtattatc taattataaa
     9901 aatagaggag aatgtgttat ggagattgat aataaaaata tcctgaaccg tttgaaacgt
     9961 acagagggac aaattcgtgg tatccaaaaa atgatcgaag acgaaaaaga atgtatggat
    10021 gtcatcactc aattaagcgc tgttcgttct agtattgatc gcgtaatggg catgatcgtt
    10081 gcagataatc tgaaaaattg ctttgagaaa cctgatagta atccagaaga acaagcggct
    10141 aaactagaac aagcgatcaa gatgattatt aaaaagtaaa ggaatgattt caatgaaagt
    10201 cgttttccat attgatgaat tagaaaaatg gtcagaaaca gggaaaaacg taaaaaacct
    10261 gataaaagct tctccagaaa cgacgattgt cgtatctgta aatggcatcg cgattaccgg
    10321 ttatctagac tctgccaatg cagaattctt agatttgcaa ggagtcgttt ttcacgcttg
    10381 cgccaatgct atgcgtgcta atcatatttc agaaagttct ttacctgaac aagtaatcgt
    10441 tgtaccagca ggtgtccttg atttagttga actgcaatcc caaggttatg cttatatcaa
    10501 gccttagtca gtagaggttg cgacagaagt gatcagcttc aagaaatttc ggcttatttt
    10561 tcgaagaagc tacttctgga aaaccgtgaa tcaaaggttg tgaaaacgct gtcctgcatc
    10621 aagtaataag aacagccaaa caaaaatagc ttctcatatt tttcgtttgg ttgggcttat
    10681 taccgcagat gcaagctttt gaacaccgtt taaggaagag gttgtgaaat cagcggtttg
    10741 acctaagtat tagagaaaaa aacgggaaaa tagctcctca tattttgccg ttttttgagc
    10801 taataccgaa ggtcagctga ttgaacaccg tttaaggaag agattgtgaa aaagtcgctt
    10861 agtttcgagt attaagaaaa attttgtgaa aatggatttc tacatttttg cataaatttg
    10921 gcttaatacc gagaaactgg cttttgaata ccgtttaagg aagaggttgt aaaatcagcg
    10981 gtttgacctg agtattagag aaacaaacgg aaaaatagct gctcatattt tgccgttttt
    11041 tgagctaata ccgaaggtca gctgattgaa caccgtttac caagaggttg tgagcctacc
    11101 atttaactcc gaatcacaag aaataatttc aaaaacaact tctcctgttt ttggttcggt
    11161 tggcttatta tcgagaagta gctactggga gtctatttat caaaaaaagg aatgcgacat
    11221 aggtcacacc ccttttttat tttctttgaa acatctttcg gtactcgaca gcatacaaga
    11281 caaaagattg taaaagaccg taagtgatga cggcaaaaat gactctcacc cacagcgttg
    11341 gtaattctgg gaaaagcagc aaaaggatca tgccaacata gatgacagca gtcgctactt
    11401 tgccatacca cttcgctcca cttaatttga tatttttaga tagcccgatt aatccagcta
    11461 caaacataaa gccttctttt atcaagaata gaccaaacaa cagccaaagc cacggtcgat
    11521 accatgccat agacaaaatc agaaaaagtt gtgtcaattt atctgcaaac ggatcaagca
    11581 ccttccctaa gtctgttacc tgattatatc tccgagcaat gaccccgtct agataatcag
    11641 acaaacctga gacaacaatg accgctcctg ctatataaaa tgactgtctc agcacaaaat
    11701 aaagataaat cggcagcaaa aaaatacgga atatcgataa aagattcgga atcgtttgta
    11761 tttctttacg ccagtctgtg cttaatttca ttttatcgcc ctacctcctc agcactcttt
    11821 tctttttatt ttagcttaca cggggaataa aaccaatgat ttcactgttt ttatttttct
    11881 ttacagacac tgatattatg agctaatttg agataatcac ttgctgtttt catcgtttac
    11941 tcttctttct tcctgtcgtt tgcgcatttc ttttactagt actttatgag ctttttttga
    12001 tcctcgctct aggtcacgct gccgtttacg tgttttataa tcgataaaca gactgtctaa
    12061 atcgtaacct gtcgggtaca gatcttctgc tgaacgcacc agacgaacac gctgacgagg
    12121 cacgtggatt atttttttgt ctaaatatac atcaatcgta tcttcaccta tatcttcata
    12181 aactaacgct tctttttggt attcattgac aaatatccga tcaccttttg caaagagctg
    12241 acgttctttt ttattctcct tcctatttgt aactgctgtc ttaggaagaa attctttttt
    12301 ctgtgtccga taggcttttt ccgccaagta acgctctgct tgatggatca atttcatcga
    12361 catatccatt ttatgagcaa tccacaaagc ttggctttct cctgtttctt ccaacccgca
    12421 actgatattt aggacgaagt gcttcccgat cgaaagccat agcagccggc acaaaatctt
    12481 catgatctcc ggcgaatttc ttgatttctc cataatgggt cgtggcaatg atcaatgccc
    12541 cttgctcata catagattcc atgattgcga tcgctagtgc agccccttca tttggctctg
    12601 ttccactgcc aatctcatct aaaagtacca acgtattcct ttttatcttt ttcaaaatag
    12661 cagcgatgtt ttgcatatga ccagaaaatg tactcaacgc attttctaaa ttttggtggt
    12721 caccgacatc gacgaaaatc tcatcaaaga cagcaagctc tgtcccttct tttgccggaa
    12781 cttgcatacc gaacatcgcc atcaaagtca ataaaccgac tgtcttcaac acgactgttt
    12841 tgcctccggc atttgctccg gtgataatca agcctcgata gtctttaccg atttcaacat
    12901 ccaatggtac cgcatgctca ggtaaaaatg gatgtctgcc ttgcttgata atgatatgtt
    12961 ccgatttatt gattctaggt gtgcttccat tgatttctcg gctaaatttt gcccgagcaa
    13021 agatgatatc taacaccgtg atcgtttcga tcaataaatc gatcacttct tcgttttctg
    13081 cgatagcacc agtcaatgcc gccaggactt gatattcttc actgatttct tcagctttta
    13141 acaactgata gtgttcattc aatttagaaa tcacagttgg ttcaataaaa accgtcgttc
    13201 ttttatttga ttcatcgata atcgttccat ctattttatt tttatagctg gctttgatag
    13261 gaatcgtata gtattcgcct ttttgaacaa tcattgcttc ttggatcatt tctttgtttt
    13321 tcggatgacg caagaatttc agtaattttg actggatttc tttttcgatt agttgtaatt
    13381 gtttgcgtac tttacgcaaa ttacgtgacg cgtcgtcact gactttatta ttttttattt
    13441 tctggtcaat caactcttct acatttatca aatcaggaag atgtttgcta taagcaaaca
    13501 acaagggtgt ctggtattga ttcttatcaa aaaactttgt gatcatccga ctgcttctaa
    13561 gaaaatccgc atattcgatc aaatcagttg gctgcagaat caaccctttt ttgacttgtt
    13621 ctgtcagtgc atcgatcctc ggcaacccca taaatggaac atgctgactg ctttctaaga
    13681 tcaagcgtgc ttctcttgtt tcttcttgac gatccaagac agtttgtagg tttgtcgaga
    13741 ccgtcatatc gctgattcgt tttttgctat agtttccaat tgctcttgct tggatttcct
    13801 ttttgatccg atcaaattgt aattgactaa tcatttcgtt gtccatattc tctccttttc
    13861 ttgaagagat acctgtccca aaataaataa gggcctagag ataaataaaa ggatagaaca
    13921 cagcttaaaa aagtcatgtc ctatccttat tagaatacaa agatattgtt ttcctgacag
    13981 gtaccgtttc ataagcacac caaataaact ttgtttattg gctattcgac ttactgatta
    14041 gataccagct acactcacaa aacatacccc attcttctag gtcttattta ttttccactt
    14101 aactatagca agacacattt tggttgtcaa actttagtat gttttataga tttacaccat
    14161 taatgaatag acacctaccg cgataattgc tccgatcatt ggacctgcga ttggtaccca
    14221 tgaatatgcc caatctgaag ttcctttatt agctattggc aataccgcat gtgctaatcg
    14281 aggaccaaag tcccgagcag ggttgattgc atatcctgtt ggccctccta atgaaagacc
    14341 gatggctaag atcaatccac ctacagcgaa tacgttcgtt ccaggtgcaa attcgttttg
    14401 tccaaaagct agcaacccaa gaactaatac aaatgtacca attaattctg tgatcacatt
    14461 tgctggataa ttccgtatag caggacctgt tgcaaacgtt cctaaaatcg cgctttcatc
    14521 tttcgttgca ttccaatgtg gcaaataaga aagccaaaca agaatggccc ctgcaaaagc
    14581 tcctaaaact tgcgcaatga tgaaaggaac gacaagattc caactaattg cccctgtcat
    14641 cgccatagct agagagactg caggattcaa atgagctggg cccatgaatc cagaaatata
    14701 gacagccatt gttacagcga gtccccaacc aaatgcgatg acaatccagc cagaagcctg
    14761 tgctttactt ttgtttaaat tcacagccgc acacacacca tctccaagca aaactaggat
    14821 cagtgtccca ataaactccc ccatgatttg agtcatatca ctgttcattt atttctcccc
    14881 ctttagttcc cgcagatctg attctgcaat caatgcttct aaacgtttag tctgttgcgc
    14941 cttctcctct tctgtccatt caaaatatcc agcaattgca tcgatcacag gttgcttgat
    15001 ctcatcaagc tgatctcgtt caaacaacag atgatttgta cgacgaatga gataatcgcc
    15061 tggggccaaa accatttctt cttctaaacc ataacgtaac cgagctgact cagcgagact
    15121 caaaccagga taaggtgcca tttcttttgc taattcaaag atacgtcggg cattagtccc
    15181 gtaaaaatca gcgatataag tagcatcttc ttcctctaaa ccggctgcta ctccttcttt
    15241 tgctaattct gtcactgttt cttctaattt agttggatcg aagtttccac ctgaaatctg
    15301 atatttttta gagtcgattt ctttcgtctc tattccgtat tcttctttta ataattggcg
    15361 tatcaatcgc aaagctcctt ctgccatctt acggtaatcg gtgattttcc caccagataa
    15421 agtcagcaaa ccatctggtt ctctttctaa agaactacct ctggaaatcg tagaaggtgc
    15481 tttttcatca cggctgtttt ccaaatggtt caacacgtct tctacttcag cacgagaaac
    15541 tttgttttcc ttatattcac ttactgtatc gacaacttta ttgaagctct tgtctgaaat
    15601 cgatccatta tctccaccat tgtaatcaga accagaatta ccaatcaaca gtggacgaag
    15661 tcctgcccag cttgcttcga tatcattcaa tgtgatattt gcttctggat agcgatgatt
    15721 gatcacatcc aatagataat ctacatcttc ttgtgtgact ttcggatccg taaaatctcc
    15781 ttggtaatcc gtatctgtcg taccaaagta agtcttgttt tctcttggaa tagcaaaaac
    15841 catccgccca tcttgctttc ctgtatcgaa gtatgtgggt tgcggtacag gcagtttttt
    15901 cgcatctaca actaaatgga tccctttagt tggacgcatt ttaggagaga ctggacgcgt
    15961 aaaattcaag tttcgtactt tatctaccca aggaccactc gtattgatca ctaattttgc
    16021 tttgatttcg atcacttcat ctgtcagcag gtcacgagct ttgacgccaa cgatttgatc
    16081 gccatcatat aaaaagccaa ccgctttcat tttacttact agataagccc catcttcggc
    16141 agcctttttg atattatcaa tcactaaacg ggcatcattg ttgcggaaat ctagatagac
    16201 acccgcgcct tttagccctt cttttttcaa aaacggttct ctttccaata cttcttctgg
    16261 tgtcagcgta taattctcat atttggttcc tgtcacattt gccagtttgt cataaaggtc
    16321 cattgctact ttgaccgaga acatattgaa ggttgttgct ccttcgccct catagattgg
    16381 caaaagcatt ggatctggtt ttgggatgtg tggggcaata ctttgtacga ctgcacgttc
    16441 accaactgtg tcagctacta cttccacatc aaatgtcttc agataacgaa taccgccatg
    16501 aacaagtttg gtcgagcgag aggaagtccc ttctgcaaaa tcctgcattt cgatcaatcc
    16561 tgttttgatt cctgatgctg ctgactggac tgccacaccg gcaccagtga ttccgccacc
    16621 gatgatcaac agatccaatt cttcttgctg cattttttga atgctatctt gtcttgtctt
    16681 gtttgaaaac atatggattt cctccttatt caaatgcttg ggtagctttt actgcttttt
    16741 tccaaccgtt gtaaagtttt tctcggcgct cttcctccat ttgaacttca aatagcttgc
    16801 cagcttcata aaattcgcgg atctcgtctg tatctttcca ataaccgaca gctaatcctg
    16861 ctaaaaatgc tgcccctagc gccgttgttt ccaagttttc tgctcgttgg atcggtacat
    16921 tcaagatatc tgtttggaat tgcatcagat attcattgtt tgctgcaccg ccatctactt
    16981 tcaatacagg aatcttgatt ccagtgtcat cttgcatcgt atcgatgata tctctcactt
    17041 gataagcaat cgactgtaaa gtagctttaa tgatatcttc ttttgtcgta ccacgtgtca
    17101 agccaaacat agcccctcgt gctgcctgat cccaataagg agcacctagc ccaacgaatg
    17161 caggaacaac ataaacctca tcttcgcttg tagagcgttt cgctgcgtct tctgaatcgc
    17221 tagctttttg caacatttga agtccatctc tcagccattg aatcgaagaa ccagccacaa
    17281 aaatacttcc ttctaaagca tagtaaacct ttccatttat gccgtaacca attgtcgtca
    17341 ataaattatt tttggataat tgcggttttt caccggtatt catgacaata aatgaacctg
    17401 taccataagt attcttgacc atgcctggtt cgaatgccat ttgtccgaat aatgcggctt
    17461 gctggtctcc tgccatccct gaaattggtg cttcgcttcc ataaaaatga taaccttgcg
    17521 tataaccata aacttctgag ttagaagtga cttttggtaa catcactcga ggaatattta
    17581 gtaaatctaa aatctcctga tcccaatcta gctgatggat attgaacaac atcgtccgac
    17641 tggcattcga gtaatcagta acatgtgctt gtccatcggt cagtttccat actaaccaag
    17701 aatcgatcgt tccaaacatc aattcgcctt tttcagctcg ttcttgcgcg ccttctacat
    17761 gatcaagaat ccagcgtatc ttcgttgctg agaaataggc atcgatcact aaccctgttt
    17821 tttcgtggaa aaattcttga tagccttcat tttttaagtt atctgcaatt ccagctgact
    17881 gtctggattg ccaaacaact gcatgataga ttggacgtcc tgtatctttt tcccatatta
    17941 ctgttgtttc acgctggttc gttatgccaa tcccagcaat ctgatttggt ttgatccctg
    18001 attcgatgaa cgctccggca ataactgact gtacagaatt ccagatttca tttgcatcat
    18061 gttccaccca actttcttgc gggaaatatt gcgtgaactc tttttgagaa ctgccgatgt
    18121 gtcttccttt cttgtcaaag ataattgctc gtgagctagt tgtaccttgg tcaatcgcca
    18181 tgatatacgt tgattctgtc attgtgattc cctccattat atgcaaacgc tttctttaac
    18241 tactcttatc atactactgt tttaaaaaaa gaaactgtca aaattttatt ctttttttct
    18301 ttttttgaca gttttttatt atgaagctat tttttaatca attcttttct tttttcagcg
    18361 tctctattct tcttttcaat cgaataatgt cgtaaggtga cgcaaattct gacagataat
    18421 attcacactg ttttttgttt ctctggcaaa atgtgatcac caaatcatat tctttacctt
    18481 cttcataact tccaatctca ataccgccga ttcccctcag ttctctgatc agatattgtt
    18541 gaaaagcaat acgataaatc ggcagacttt ctaaatcgat tccaatcact actggttttg
    18601 caatataaaa atcgatcatc aaaagaacat tggcatactg gatcatcagg taactaagct
    18661 cactatcttc agctcttttc ctcggcaatt gctgcaccgc tgtttcctgt aatttcgcca
    18721 gtaactgtgt taaggaatgc ccgagcattt tctgctgctg ttccaaaagc cgatcacgct
    18781 catagacttc gatctttcca acaaagaaaa accattcatt gttgatctgg gaaatttggt
    18841 agcctactgc cttttcttct ttgattttca aacgacgagg acgataatgc cataacatcg
    18901 tttcccgaat gtaagtatct aaaacagcgg ttggtaactt tttgctccgt gtcaaatcat
    18961 actgataaaa tatttcttcg tctactatcg aaaaagaaac aaaaaagctg taaaacatca
    19021 ttgtttcgta tacattcagt tctgctgctg ttcgactaag atgcatcacg atgattggat
    19081 cgattgcttg ataaagccgg tccgactgat agagtaattt cttttctttc aaagttgcgt
    19141 acgtactttt ttcattcaat agtctttttt tcgtaatccc gagccagcaa gcaatctgtg
    19201 cttctgctga tgccgtaaac gttgtttcta atacacggtc caatcctaga atgaacggtc
    19261 ttttttctgg tgtattttgc aaaaaaagcg gtcgctgatc ttccgggata tattggaata
    19321 gttcataata aaaatatcgt atctgtagct cttctccgta taattgaccg tttttgattt
    19381 gtaatccaaa ttcctctaag agctggttca attctttgat ctttcgaaaa acagaagact
    19441 cactgatatt aaacgctgtc gctaatcgaa cgatggatac ttgctgatgt tctaataaat
    19501 agacgagcat cttaaacttc aagctttcct gcaataaaaa caatagaatt ttttccaaac
    19561 ttacatcttc gtccaacaca agcagcgcct gatactcatt tcgctgtact tctacttttt
    19621 tttccatcat tcggccaatc aattcaatat cttccaaata ttgatcaaac gaggcttttg
    19681 acagttggac atgctctctc aaaccattga tagaggctgt tcccccttgc agaacaaggt
    19741 ttcgcaaaat caggatctgt aactgctctt ttctttctaa taatgcttca atcttcatag
    19801 gcagtattcg ccacctttga ccacaccata tctggcacac ggtattctgg ctggaaaaat
    19861 agctcatgtt cttctaactg ttcaaacgtc catccttctt ctactgcaat tgtcagttgg
    19921 tcaagaaggt ggaacatctc ttttttgctt tggagctgtg ctccaagaat ctttttttca
    19981 gaatcaaata gaaatttgag tttaactttg ctttgattta ttgctgtaag cttgtagtct
    20041 acgtatcctg tttcaatttt ttggggataa aaaatgctct cttcttctgt caaacctacg
    20101 cttcctaaga aatagccaaa ccactgattg ccgactggac gataggttcc ctgatccttt
    20161 gtctgtgagc cactaattgt cttgctgaca tttcgtgctg tacggacagc gttatttact
    20221 agagaaacat acattttttc atttgttgga cgaaaagtta cttggatcgc atcgccaatc
    20281 gcataaacgt ctttttgaga cgtttgcaag tagtcatcta cccagatcgt tccatcatca
    20341 ttcaatgtta attgttcttc ccacatctga ttgtcgggac gagaatgatt agccagcaaa
    20401 acaagatcat agttctcctt tcttttttcc gtctctacaa tagcggtttc attttcggtc
    20461 aaagacacaa cttgttcttc taaaaaaagt gaaagccctt gatttttcat ttcctttact
    20521 aacggttctg tcatttcagg atctagataa cgaaaaagaa ttgactttcg gctttcgtaa
    20581 agatggattt gtttttttct tgatgccaac ccttcagcta tttcgattcc tgcttgtcct
    20641 gcaccaataa ttgcaatttt ctttgcattc atgatctttt gctgaagagc cgttacgttt
    20701 tgtatctttt taactgggtg aataagcgtt gattctgcag aaatatttcg aaacttttga
    20761 tttgaaccag tcgctagaat caaacgatca aaatccaatt tttttcctcc tgctaaaacg
    20821 actttttttt ctttcaatcc tattactgtc ttttctgtgt aaacatggat tcgatagcgg
    20881 tcagtcagtt cttctttcgt gatccaagaa agttgttttt cactggtaac ctttcctttt
    20941 aatatcaaat tgaacccgct gggaatccat cctaacttcg attgcttttc aatcaaatat
    21001 atttcacttg ttggcatcaa ttggcgaaga taaatagccg catgaatccc tgcaaacgac
    21061 ccgccgacaa ttacaatgtt catctgcatt cctctttctt ataaggtgtt ctactgctaa
    21121 cttctcaatc agaaaacaca gtattcataa ctcgctttca accttatgat acatagcgat
    21181 gcagcagaat caaccctctc catcataccg tgttctaggt ctgcttctca ataatgtact
    21241 agtggaccta attgaagagt atcatttctg aagagatgtt tcaaaaaatg tactcttata
    21301 aaagaaaaaa actcgccttt tacgtacctc tgtccaaata ggaaacagaa aagaacgaaa
    21361 agacgagttt tagcgtgtgt aatgtaatag ttagaataga cacatcattt atttttaatt
    21421 ctttaatgct tcaacaactg cttcgcctaa ttgatgagca gattgaggat tttgccctgt
    21481 aagaagccgc ccatcaattt gaaccacttc tgaaaaagct gctccagact gatagtttgc
    21541 tcctttttct ttcaaagcat cttctgctaa gaatggtact tcattcgttg taccatttgc
    21601 ttcctcttct tcattactga acccagtcac tgtacgtcca gctaaaatag attttccaga
    21661 ctcatctgta actggcaaca atcctacaac accgtgacaa acagctgaga ccagcccttc
    21721 atttcgatag atttgtgaag cgactttccc aagatctatt gcttctggaa aatcccacat
    21781 tgttccgtgg ccaccagcat aataaaccgc tgcataatca tttgcatcca catcatcagg
    21841 acgtaaggta tttcccaagg cccgatttct gaaatcttca tcttcataat aggcccaatc
    21901 gatttcatcc atattcaaaa cactttgcgg ttctaaaggt acatatccgc ctttaggact
    21961 gacaaaatct gcatcgatcc cattttttac taatacatca taaaaatgag tcagttcact
    22021 taaccacaaa cctgtaggac gctcgatctt ttcgtacttt tctacatttg ttaatacgat
    22081 caatactttc attttcccat cctctttcta taaaaattgc gttattgata acgtgctttc
    22141 aagaaattat ataatgggct tggcaaataa agctctttat atgcttcttc aaaaggtttc
    22201 gttacaaatt catcctcacg gttttccaat aatttctctg cccagtcagg atcgacgatc
    22261 atttgctgtc caattgctgc aagttctgca tatttcaata cctgctctac atcttcgcga
    22321 ttgcggatcc caccgacacc aactaaaggg atctttcctt gaatcgtgtt atggaccact
    22381 tcaagaatcg atttatcgtt gtatttatca gaacgagcta ttcgatcata catatttaat
    22441 gaaacatgca gataatctag ttttgactca cgtaatcgtt caagaagcca gatcgtgtct
    22501 tcaaaacgga tacctggtgt ttcatattct tctggtgaga aacgataccc tacgatgaat
    22561 ggttttttag catattgttc aaccgctgag aaaaccgttg ttaataattc ttcaataaaa
    22621 cgataacgat tttccaagct accgccccat tgatcctctc gtctattcga atgcggagag
    22681 aaaaattgtt ggatcaaata cgtatttgct ccatgcaact cgattccatc aaatcctgat
    22741 tcaatcgctc tacgtgtggc tttgccaaat gcttggattg tttcaacgat ttcttcttca
    22801 ctcattgctc gtggtgtttc cgcatcagga cgttttgctg caactgcact tgcagataca
    22861 ggctgttctc cagataaagt tgctcgactt gtcattcgtc caccgtggaa aatctgaata
    22921 atcgcttttg cgccttctcc ttggattgct ttcgctagct cacttaatcg aggaatcatt
    22981 tcatcatgag cgatgctgag ttcacctggc cagccttttc cgatatcttg gacatttgca
    23041 gcaccagtaa taacagcacc aagaccttta gaacgttgtt tataataagt gatctcatct
    23101 tcggtgatca ctccattgaa aaagctgagt tgtgtcgtca taggcgacag catcaatcgg
    23161 ttcttcaatt gaactcctga aggtaaagta attgattgat caaattgtga cattgtcatt
    23221 cctcccataa ttcgctctga ttaacctatc gagaatcatc tatcattaaa ccactcgatt
    23281 agttgtacat gcaactaatt tgcttaaaaa attttatttt ttttgtaact tatctgttgc
    23341 atcattcatt gtctgggcta attctttact tgcttcattt cccaaaactg ctttgtatga
    23401 ctgatgaagt tcttcccagc taccatcgac ttccttagca aattggcgtc cttgttctgt
    23461 aagataaact aatgacaagc gaccttcgct aactgttttg acgtatcctt tatattctaa
    23521 tttttcaaca aaacgagcaa tcgttgatgg ggcggaatag agcatttgtg caatatcttt
    23581 ttgtggtatt ccatcttttt cttttaatat caataaaata aaaccataag ttggtgccag
    23641 acctgtcctt ctaaacgctt catcagctaa tttattcaac atgcgatcta gctttttcac
    23701 gctaaagaaa agacattcat ctaacgcttt ttcaattggc ttttccattt catcccctcc
    23761 attggcgtac atatcatagt atgattttta ttttttgtca aaaaacacgg aataatgtcc
    23821 gcctcctctg tctagatttg aaaaaaagaa gtagccatct tcaaagaatt tcggcttgtt
    23881 ttcgacaaaa ctccttctgg aacaccgtga atcagaggtt gtgaaaaagt cgtttagttt
    23941 tgagtatgaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa atttggctta
    24001 ataccgaaaa actggctttt gaacaccgtt tatatcagag gttgtgaaaa agccgtcctg
    24061 catcaagtaa taagaacagc caaacaaaaa tagttcctca tattttgtcg ttttttgagc
    24121 taatgccgat ggtcagctga ttgtacaccg tttattgaga ggtcgtgagc ctgccgttga
    24181 actccgagtc acaaggagca atttcaaaaa acagcttctc atgttttatg actatggtga
    24241 cttgtgatcg aggagttggc aggagaatac tgtttattcg taaagaaggg gctgtgacaa
    24301 gacttttgtc acagtccctt atttcctttt attataggat ttcgccattt gattggatga
    24361 cttgtctgta ccaatcaaat gattttttct ttaaccgttt ctttgtgcca ttcccttcgt
    24421 catcttgatc aacatagata tatccataac gttttgacat ttgtgaagta gaagcgctga
    24481 cgatatcaat gcagccccac gacgtataac caaataaatc tactccatct ttgatagctt
    24541 ccttcatctg ctcaatatgc tgcttcaaat agtcaatgcg gtaatcgtct tgaacggttc
    24601 catcttcttc tactttgtct atagctccga gtccgttttc agctatgaac agtggttttt
    24661 gatacctatc atataattga ttcaaagcaa ttctcaaacc cttaggatca atctgccagc
    24721 cccattcact tgatggaaga tacggatttt taattcctcc agttattaag tttccttgga
    24781 ccccttccca attttctgga tgagcactga ctgcactaga catgtagtag ctaaaagaaa
    24841 caaaatcaac agggtaggct gctaagattt cttcatcacc tacttctatt tttaagttga
    24901 cacctttttc ttcaaacatt cgttgcgtat acgctggata atatcctctt gcttgaacat
    24961 cactaaagaa caaagaagtt ctttgctgct caattgtttt ttgaatatca tccggatcac
    25021 aagaatacgg aacaagaagc tgatagctga tcatacaacc gacttgtgaa cctttgataa
    25081 tttcatgaca agcttttgta gctaaagcac ttgctataaa ctgatggtgt gcaccttggt
    25141 atttcgcttg ttccaaatgg ggatggtttt cttcaattac tcctacgcta acaaaagggt
    25201 gatgttttac acagttgatc tcattgaaag tcatccaata ttttacttta tttttatagc
    25261 gtttgaatac tgtttcagcg aaacgaacgt aaaactgaat aagctttcga ttaggccatc
    25321 caccatattc tgtaaccaaa tataacggca tttcataatg ggaaagagaa acgactggct
    25381 caatcccata ttttagacat tcgtcaaaca cttgatcata aaaaaataat cccgcttcat
    25441 tgggtgtttc ttcatcacca tgaggaaaaa ttcttgtcca tgcgatagac atccgatagc
    25501 atttaaaacc catttcagca aataaacgga tgtcttcttt aaagtgatga tagaaatcat
    25561 ttccgtgtct tttaggataa taatacttgc tgttgggatc ctgcgccgct tttacttgct
    25621 gatgcgtcat catatgccat tgatctgtcc atttttcttt aggtaaatcg ctgtcgaatg
    25681 tatacacatc agacacagat aatccttttt catctacatc ccatgcacct tcacattgat
    25741 tggccgctgt cgctccgccc cataaaaatc cttctggaaa ttttttcata tcgatccccc
    25801 taaacttgct agttttcttg tttgattatt tttcttatga ctagtaatga tatttttgaa
    25861 tctactacta tttgtaacaa taaccattgt ggtcggatca tatccctctt tgattaattc
    25921 ttgcaaatct acttccatca ataaatcgcc tttacgtact ttttttccat cagatactgc
    25981 actttgaaag tatttgccat ttaaattcac tgtatcaata ccgatatgga tcagtatctc
    26041 tgctccttct tcactgacta ccccaattgc atgtttcgtt ggaaaaactg ttgaaacttc
    26101 cccgtttaca gaactgtaga tttttccgtc cgttggaatg atcgcaaacc cttctcctaa
    26161 agatccacta gcaaatgttg gatcgtttac ttcagataaa tcaattattt cacctgctgc
    26221 aggagtactt aacactatat cctttgagga ttcttcttcg atttcatcct tgtagccgaa
    26281 aagaaaagtt agcatacagg aactgatcat cgctaagaaa taactagcaa ctagccaaac
    26341 aaatccttca ccaatataaa tcgggaaaga caaaatactt cttgtagcaa ctgccaaagc
    26401 accggctcct gcggcaccag ctactccacc aaatatagct ccacatatac aagcaatgta
    26461 aaatggtcgt ttatatttaa gagtcacacc ataaacagca ggttcagtga ttccaaataa
    26521 tgctgtgata aatgcagata aagataaact ttttactttc ttatttttcg ttttcaaaaa
    26581 taccccaagt actgctcctg cttgcgcagc tacggcagtc atacatgctg gacctagcgt
    26641 atcacgaccg tagaaagaaa gattgttggt cattactggt acaagtcccc aatgtaaccc
    26701 aaaaatcact aacacttgcc aaaatccgcc gataacagca ccagataaaa cagggctaaa
    26761 gttataaaca gctaaatatc cagctgcaat cagctgactt gcataatctg caattggacc
    26821 tacaactaat aaaataattg ggaccatcac gatgatacaa atgaatggtg tcaataaatt
    26881 tcgaatactt tcgtgcaagc ttttgcgaat cttaggctca atataagaaa gaacccatac
    26941 agctaaaatt gctggaataa ccgtacttgt ataccttgct agaatcacgg gcatccctag
    27001 aaatcgcaag gttatgctat cactatatgc actgacaatc gtaggataaa cgagagctgc
    27061 tgcaataacc atagcgatga aacgatctgt attgaacttt tttgcagcag tatatgccaa
    27121 aaatataggc aagaaataga aaaatgcatc agctgctgca ttcaaaatga tataagttcc
    27181 tgattcagta gttaataaat tcaaactcgt acataaggcg agtactcctt tgatcaaacc
    27241 acttcctgcc aagacgccta aagtcggggc aaaaacagcg gcgatcatgt ctataaaaga
    27301 gtttaaaatg ccttttttct cagttcctct ctttccacta gatgcatcat cttctagttt
    27361 catttgtttg gataatacgt catacacttc atttactgca tttcctacta cgacttgaaa
    27421 cattcctcca gcttcgacta cagtgatcac accttctagt tgctctacct tctccttatc
    27481 tgccttctta ttatctttta gggtaaagcg taaccttgtt gcacaatggt aaacattgct
    27541 gacattttct tcgcctccga caaatcggac aatatctttc gctaattgtg aatagtccat
    27601 ttttacttcc cccttgttaa ttaatgccgt agcaatcctt cttacaagat aagtatagtt
    27661 cttagaaaac ggtttctcca ttcaattagt accccttcta atagcgaacg aaactgtaca
    27721 atagtgtgta tggtttactt agccgagcaa atcactcact gcttgaaaga aaacaagtat
    27781 atcgtttctc ttatagtcaa aggagacaaa aaaaacacgc ttgatcttcc tttgctgatg
    27841 tataagtggt atctcaagct gatactctgt gatttttgtt tctacttggc ttttgataag
    27901 acagcacaac aaagtctcgg catttaacgg aagcataact aggtcagaac taggagagat
    27961 tagttgctga atggaatcta tctttttatg gatttttatt tgattttctt ctttgtagta
    28021 atgatcaagc agttttgttt tctttttttc ttcaactatg gtcagctttt cacgcaaaat
    28081 ctgataaatc tcccaagatt caaactcatc tagaacacta ggtagataaa tcaaatcatc
    28141 ccactcaaaa ataagctctt gctcagtagt caaaattgca tcatattccg ttattttgtt
    28201 tgattgattt ttgaaaagat acactggttc tatccggcat tctttgatcc acatgttcat
    28261 actttgttga atctgttttt ttagtgtatt caaaatacta attggctggt tactaacaat
    28321 cagtatctcc ccttgttttc gaaaaggatt caacgcttcg gcaagataca aaacaaaata
    28381 agcgctttca gctaaattgg gacgtaattg aaaatgtatt gggaagaaac gccgaattaa
    28441 ataaatttct aaaggataac gggagatgat cttttgtaca aaatgattca tggtattatg
    28501 tccggcatcc atcctcgttt tcatttgttt gatatgggtt agcaaaaatt ctgtttcatg
    28561 gaacaataac gaaacaggaa gatctaaaaa acgaataatc gtttgctcaa atgctcttag
    28621 attcttctct atttctttgt ttttttcatc attaatcatc aaataattta actctaatag
    28681 tcgattttca attgcttgtt tttcttgatt ggaaaaagaa tagggtagtt ctgatgataa
    28741 cttttttgtg agggtctcta ccatcggaat aaataatgta tacctgtttg ttttttctaa
    28801 aagttcttct cttcgaagaa tggtcaaaac gagataccga gaaaatcgta tgaagtcctc
    28861 tcctgaaaca atataattat atgatataag aacctctttt aatgtctttt ggacagtcgg
    28921 catgatattg gaaaaaattg tactgatttg ttgaggaaac cttgcgtgtt ggatcaccgt
    28981 ttctgttcca acaagagata agaaaattct ttttgaattt tcttcagcaa tgatttttag
    29041 tccttttgtc gacgaaactt ccagttctaa tttaggattg cgctcgatcc aacgcaaaat
    29101 cgtttttatt tgtaatgata cggctgtttt tgaaagaaag aaaatgtctg ctatcttttg
    29161 catactgaca tacccttttt gaaacaataa gtacgtcaca atagttgaag ctcggaaatc
    29221 catgctggaa tagattttat gtttcgtcac ctcttgtatg atttgctgtt tagtttcaga
    29281 ttcaattgat tctaaaaaat aacctgaacg tttatttgac tggataatga ttttcttctc
    29341 gtagatagca agcatatttt tgatttcagt ttggatcgtt ttcttgttgg tacctaaagc
    29401 ttcagctaat tcttcgcttg ttatccaatg attcttattt cttaatacta caagaatttg
    29461 gagttgtcgt ttatttaagt ccatttgctt acacctcctc tttgttataa acatacagga
    29521 aaaacctctc aaatgacaat agtcacttgc agaggtttaa aaatcatttg aagaaagatc
    29581 tatattcaag gtttttacca ttagactgga taatttgttt ataatattca aaactatctt
    29641 ttttcaatct cctaaagctt ccgctcccat cattttgtct atccacataa ataaagccat
    29701 atcgcttcct catttcccct gttcctgccg atacgatatc gattggtccc caccataagt
    29761 aaccaatgat ttggcaacca tcttttattg cctcagcaat ttcctgaaga tgtattttta
    29821 gatactcaca acgttttgga tcgtggattc gattcaattc atctggtgct tcatctagtc
    29881 caatgccatt ttcaactatg aataatggta attcataacg gtctgtcaat tcattcatga
    29941 cataacgaaa ccctagagga tcaattggcc aactccaagg ttcgggagat ttttccttca
    30001 aaaagggatt atcgaaacca tttgctccgc cagtatctac gcgcatttct gcttcttttt
    30061 tatagacagc gctacgataa taactaaatg caatataatc tgccgtgtta gcttgcaagc
    30121 actgttcgtc tccatctaac attactggtg cacagtcttc ttccttccat gtcctgtaaa
    30181 tataagctgg ataatgccct cggcacatga catcggagaa aaaccatgaa ttgcgtttaa
    30241 attgcagtga accaaataca ttctctggat cacagtcata tgcataagtc gctaatgcag
    30301 aaaagctaca cataataccc atcttagcat ttggatcgat tttctttaat aattgagtag
    30361 cacgggcgtt cgctagaaac atatgatgac aggcttgata aatcactttt tttgttagtg
    30421 tagcaatggg ttcaagttta tctctagcag ggaatgtaac caatccagca gctgcataag
    30481 gcatacgaaa agcattattg atctcattaa aggtcatcca atatttcact ttcccttggt
    30541 agcgttcaaa gacggtctgc gcataaaatt caaaaaagtc gattaatttt cgattttgcc
    30601 accccccata ctctcctacc aatgctaagg gggtttcata atgactaaga gtaattactg
    30661 gctcgatatc atatcgattt aatgtattga ttagtctgtc ataaaatacg agaccagcct
    30721 catttggctt ctgttcgtct cctcttggaa atattcgtgc ccatgagatg cttgttcgaa
    30781 aagcttttaa acctaactcc ttcagtaaac gaatatcctc ttcaaatcga tgataaaaat
    30841 caatcgcttc atgagaaaga taatggagtg attcgtctaa agcaatctcc caaatctttt
    30901 tttcttcatt ccactgaata ctaggatgct ggttcatgat cccgataagc gtatctgtca
    30961 cattaggtaa tttgccgtct tccaaccatg caccttcaca ttgattagca gctactgctc
    31021 cgccccataa aaaattttct ggaaatattt cctgatccat aactatctct cctcgctatc
    31081 aacaatttct tacttgaagt atagctcgat tcatcaaaac aagatattct aactatttcg
    31141 gcagaagaga agaaaaaaat tgaatgctac tcttcatttt taatatttat atataacaaa
    31201 tacataagac gatccccgaa agaaaacact cgttaaccat ctaagcaaac gatttatttt
    31261 acttccaaca caaatgcttc ataaggtcgt aaatcaactt tgccgtctgg taactgtttc
    31321 agaggataat ttgacacaat aacccttaaa gcttcacgtg gaatagtgaa cgtctgcttt
    31381 tcactggaca tattacaaat aacgagccat tgctggtctt catatatgcg agaataggca
    31441 aagacatgct caggcgtctt ttccactaat tcatagtctc cccagacgat gatgtcatgt
    31501 tttctacgca aatcgatcaa tttcttgtac atataaaaaa cagaatcttg atctgctaac
    31561 gcttcttctg cattgatttc tttataattt ggattgactc gaagccatgg aattccagta
    31621 gtaaacccag catgttcacg ggcgttccat tgcataggcg tcctagcatt gtctcttcct
    31681 ttcgccttga ttgattccaa gatttcctct tttgtaaatc cagaagaaat cctttcgtta
    31741 tacatattga ttgtctcaat atcttttgct tccgcgatat catcgattgg gcaattcgtc
    31801 atgcctaact cttctccttg atagatataa ggggtgcctt tcatcatatg gagcaagata
    31861 gcaaacatct tggcactttc ttttcgatac acatcgtcat ttccccaacg agaaacaatt
    31921 cgcggaagat cgtgattatt ccaaaataaa ctattccatc cttcatcacc taaagatgtt
    31981 tgccatttgg ataatacttt tttcagttca gagatactca agggtttcaa atcccatttg
    32041 tctttgcctt cttgctgatc taatcctatg tgttcgaatt ggaagaccat ggataattca
    32101 tttcttttag gattggaata aagtttagcg atttcggtag ttgctcccca tgtttcgcca
    32161 acagtcatca gatttttgtc accaaaagtt gcttgattca tttcctgaag atactcatgc
    32221 agtttaggtc cattacttgt gatttcttta tccggttctt tgccaatcat atcgatcacg
    32281 tccatacgaa agcccccaat acctttatcg atccagaaat tcatcatttg ataaatctct
    32341 tggcgaacct gtttattttt ccaattcaaa tcaggttgac gcttgctata caagtgcaga
    32401 aaatattgcc ccgttttttc atccaactgc caagcagaac cactgaatat tgagcgcaat
    32461 ctattaggct cttttccatt gaccggatca cgccaaatat aatagtcccg ataaggattt
    32521 tctttatttt tacgggcctc aatgaaccac ggatgttcat cagaagtatg attcaccacc
    32581 agatccatga tgatacggat atttcttttc tttgcttctt caatcagttc ttccatttct
    32641 tccatcgtac cgaattctgc catgattgct tgataattac tgatatcata cccattgtca
    32701 tcattcggag actcgtatac tggagaaagc caaattgcat caatacctaa atacgccaga
    32761 taatccaacc tggaaataat cccccgcaaa tctccaatac catcttgatt tgaatcttga
    32821 aaacttcttg gatagatttg ataaacaact gaatggtgcc accatttttg ttccatgatt
    32881 ttcctcctat aaatggatgt ttctattttt tgcttaatca tttattttag tctctctcac
    32941 taaaaattgt aacgctttca aatagctatt gcaccttgtt atcggtaaca gaataaaaca
    33001 aaaaaacagc tgaggaaatt ccctcaactg ttttttgaaa ggattgattt atgcagggaa
    33061 gaatgctgat ttcacaactt ctttataaac ggtgttcact cagctgtccc ttggtattag
    33121 ctcaaaaaac ggcaaaatat aagaaactat tttttcgttt ttttctctaa tactcaggcc
    33181 agaacgctga tttcacaacc tctttatttc acattcaata tccacatgaa tcccacaaat
    33241 acaaagaaca gaccatacat gattggagat acttcttttc ctcttttagc tgctaacatg
    33301 gtaattggat agaagataaa acctaatgca atcccatctg aaatactata agtcaaaggc
    33361 atgccgatca agatcaagaa tgaagggatc gcaatctcta agtttcccca ttcgatttgt
    33421 cgcaaagatt gtgccatcaa tactcctacc ataatcaatg ctggtgcagt tacttgtgaa
    33481 gtaacaactg atagtaatgg agaaaagaac aagcctaaca agaagaaaat ccctgtagta
    33541 atagctgtga tacctgaacg tcctccaacg gcgataccag ctgatgattc tacaaatgca
    33601 ccaacaggag aagtaccaaa taaggaaccc gcaagcatag ctgttgaatc agaagctaaa
    33661 gctttcccta cacgaggcat cttgttgtct ttcatgaaac ctgcttggtt tgctaatcca
    33721 actaaagttc ctgctgtatc aaagaaagtc acaagcaaga atgtcagcac tacgacccac
    33781 atttgaagag aattgatgtc ccctacatgt tttaatgcaa ctaaaaatgt tggtttcaaa
    33841 cttggtgctg cggcaacaat ttcagaaggc attggaatca aacctgtgac cagacctaaa
    33901 atcgttgtag ctgccatccc gatgaagatg ccacctggta cacgccgaac aagcaagata
    33961 gcagttacga ctaatccaaa aatcgtcaac caagtagaac caacgttgag tgatcctaat
    34021 cctactaatg tagattcatt cgaaacgatg atcccacctt cgctcagacc aaggaaagcg
    34081 ataaatagac cgataccgcc agaaatcgca tatttcaaat ctgctgggat cgcatcaatg
    34141 atcatttccc gcagcttgaa gattgtgatc aaaataaaaa tcaaagaagc aacgaatacg
    34201 ccggctaatg ctgtttccca tggaatcccc attcctaaac aaacagaata agcaaaaaaa
    34261 gcattgatcc caagagcagg tgccgttcca attggataac gggctaatac ccccattaaa
    34321 atacagccga tcgcactggc taatgcagtc gcagtaaaca ctgctccttc atccattcca
    34381 gaagcaccta atacagttgg atttacaaat aagatataag ccattgagat aaatgttgta
    34441 aatcctgcca gtacctcacg ttttaccgtg gtgttcaatt tttctatttc gaagtaggaa
    34501 agtatttttt ccttcatctt ccatccccct aaatccgaac attataaata aagtccgtta
    34561 aaatttcgat ttattaataa aacaacgtgt tttatcatat actaaaacaa aatataagta
    34621 aacaaaaaat tcggatttta agcaaaatcc gaacgtttca tctgattaaa tccctttatc
    34681 tcaaagttca tgaacattca ctttggatta caagaatttc tttattgtgt taataaagtt
    34741 tcttcattct agtctcaaaa atcaatagtg tgatttgatt ttaccatttc gtttctgtta
    34801 ttctttgatg aattcttttg tatcgacagg attcagatta tttagtccgc caaaacctgt
    34861 gtcgaccatt tttccccagc cttttgtctt cttttttcct gatttgagat tctttgctcg
    34921 aagcttctca ctatgttttg ctcgccgata cttcaaataa ccttctaatc ttcccattag
    34981 atccactgtt tttcctcctt tttattcaat cagacgactc agcttttcct ataacaaaat
    35041 aattgccttc agggtccgaa aaattgaaag tcaagacgcc attgttgtct gtgatttctc
    35101 ctggttcttc catgttctcg tagatagtct caaattcgtc cgtaaaaaac actaaagaag
    35161 gcggtggtcc taatacttct ggtgaaaatt tctgcacaaa tacttttggg aatacgccta
    35221 attctaattc ttttgagatg ctgagtacaa tactcttaaa ttcttctggc aattcgattg
    35281 tttcagaaat ttctgcacca attttttcta caaagaatcg gcaaatcatc tctacgtcat
    35341 tgacataaag catcacacgt gttttttgta tcatcgtttg acccttcttt ctatttgatt
    35401 ttctttcctg tctataaact gattccatta tatcacgaaa gcgcaaacac cttgtgtttg
    35461 atactattca agaaaaataa agcaatagtt tctaggattg ctttcctcca caaacttttt
    35521 ttacaaaaaa aaaaaaacgg caatgaatgc cgctcttctt ttttcgattc ttttataatt
    35581 ctgcttctga ttcatctttg gttagtgtgt gtctaacact agttacgcca aatgtttgat
    35641 cgattttttc cttcaacgct tcgattgcct gtgcgccacg tgccgcttgt tcagcattga
    35701 tggattcaat gaccagcacc gctgcttgtg tttcttccgt cagcatcgtt ctttgggctt
    35761 cacgccagtt gagacagcga cagatcgctc cttgttcatc atagtagatg atttcttccg
    35821 gaagtgccgg agcatcggaa tctgcaccta aaggcaggaa tgattcgcca ccttgggctt
    35881 ttcctaaatg cagatttcct gcgatctttt cgatatcttc tccgccgcaa ggtacgccaa
    35941 aagacaagga aatactatta tagatatcta caagcggatt gattgggaaa aattcccgtt
    36001 cttgatttgc acgtttcagt aaagcttcaa tagaagaacg tgcacctttt ttagtcttga
    36061 atgtggtaaa tgcctgacgc cactgagcaa tgacttcgtt ttggctgaat gtttcttctg
    36121 taaggtaatt tcttgcttct tttgctgcct cattcaataa ttgatgatac gtttcttcac
    36181 tttcttttgt tacgtggtta tcgataccat ttacggataa gatttcgatg gttgcttcag
    36241 gaaatacttc ccagaaattg gtatccacta ttactttttt catatgttca cacctcttga
    36301 ttagaataat tgcttgatga atagatagtc tgtttgggga tcatctccca tataaaaaac
    36361 atgttcgctg tatttgataa atcctttttt acgataaaaa gcgagcgcaa catcgttgtg
    36421 ttcccaaaca ccgagccaaa tggctgtttt ttgcttttct tttgcttttt caatagctgt
    36481 atctagtaaa aatgtaccga taccttgatg tttaaatttt tttcgtacat agattcgctc
    36541 gatttctaaa aaatgatcag ccatttcctc agattgactc gttcctgtat ttagtttcag
    36601 gtaacccgca ggctcttgat cgatactcac taggaaaaaa tcggtttctt tatcttttag
    36661 ctcttctgta agtttttctg gcgcataagc ttgtgtcaag taatcagcta aatgcttcgg
    36721 tgaattatct tttccaaatg tatctgtaaa tgtttcgata ctgatttgtt gtagtaagtc
    36781 tacatctgaa ggcgtcattt ttcgaatttc gtatttattc acggcttctt cctttcaata
    36841 gatcctttta ttgccttttt tgacaaaatt ccaatcttcg ctaacattct tgcatacttt
    36901 atttaaaaga tattccaatt gttttgcttc ttcgtcagaa agaccattta atgcgactgc
    36961 attggagtag ttgttttctc gaatgatgaa aggatagatt tctgctcctt ttttagtcgg
    37021 atagagtttc ttgatttttt tattttcttt atcttccaaa cgttcgatca tcccattgga
    37081 ttctaatttc ttgattgctc gggcagtcgt tgtacgatct actttgatca tttctgctag
    37141 tttttcttgg atgattccag gattttcgca aatacgtact aaataaagat actgtcctct
    37201 tgttaaatcg acttctttga attcgatatt gctgatagaa tctagcgctc gggcaatcat
    37261 tccgatgtct cgaaggattt cagccatttc atcccacctt tattttgttt gtaagactag
    37321 tttacctctt tttgttgcaa atgcaactat tttcaataaa aaaatcaagt tcttttttaa
    37381 taactagtaa agtatattct tttcttcttt tacgaaaaag ctcaatcaca atagatctgg
    37441 tttttgtttt ctaaaataag taactgtttc ttcgttgttc gcttttctga tagctttggc
    37501 aactcttcta tactaaaaaa tcccatgttg gaagtttcat tattctcaat aaacttggcc
    37561 tcttcaccgt ggatcgcaca agcaaaaaca agtttataat actgaaaaag ctgtggaata
    37621 tctttttgtt tattcgtatc aaaaactgct cgcaactgag tagtttctac tgttaatcct
    37681 gtttcttcat agacttcttt acgcacattt tcttcaggag acaatcctat ttcagcaaat
    37741 ccaccaggaa gtgaccattc tttcgtccgt ttatcttcga ttagtaggac cttgccatct
    37801 tttttgatat aggcacgaac atctactttt ggtgttggat gtccttctcc aatcgttgtg
    37861 agcgctttta tttcttcgaa agtttctttt cctatttcac tgatcagttc caatgaaatc
    37921 gtccggatct cctcaaaacg ctctttatca aatacatctt ttccataata tagaccagct
    37981 tgagaaagtg cgagtagtct tttgtattga gccaacttct tgttcattct cttcccttct
    38041 tttttagcaa atgtatgact atgtaatcaa gtatagaaga ctagtttaat tacggcaaat
    38101 aaatataatg aaatctgcaa taaataaaag aatgttaaaa aactcgagaa caaaagacat
    38161 tcgacttctc tggggctgtg aataaatggc gttcaaaagc ttgcatctgc ggtaataagc
    38221 cccaccaaac gaaaaatatg acgagctatt cttgtctggc tgttcttatt aattgaggca
    38281 ggacagcttt ttcacaacct ccccactaaa cggtgttcaa tcagctgatg ctcggtattc
    38341 actaaaaaaa cggcaaaata tgaggagcta ttttgccgtt tttttctata atactcatat
    38401 taaaacactg acttcacaac ctcttcctta aacggtgttc tcctaccaac tcctcagtca
    38461 caagtcacaa tattcatcaa acatgagaag ctgtttcttg aaattgctcc ttgtgactcg
    38521 gtgctgaacg gtaggctcac gacctctcgg taaacggcgt tcaaaagcca atttttcgat
    38581 attaagccat ttctttgtta cttattgtca ttgatttttt ttctaattta tccaatagtg
    38641 gagtgtaatc cacttgagta tagtctgtta catcaaattc atatagtgtt cctaactgga
    38701 atttgtcata ttttcggtcg gcgatgatct ttttgaatgc ttctttcgta atatgattgg
    38761 tccctttcgt tcgatcttcc aatacgtcac catagtgata atgatccatg atatagctca
    38821 aatgacgatc tgattcacga tcacgtttgt accgacgttg ccataacact tcaaagtctg
    38881 ctgttaggcg aatcgtgatc acctgatatc cgtatctatc tgctaattct tggagtcgtg
    38941 ctttttgctt atcactaaaa ggatactcgg acacaatcac tttctttcct gcttccatat
    39001 acaaatcaag tgctttgtaa tagaaaggat aaactttcgt cctttcttgt tccgcccgtt
    39061 cttccaaatt gttaaacccg atggattctg catagtattc cttgatttca tcgagcgtga
    39121 tggcatacat atctggaaat cgttcccgta gtttattcat aagatatgtt tttcctgtac
    39181 caggactacc agccagaaga atgaaatact ttttcatctc atttatttcc taagttaatt
    39241 ggactttcca agttcataaa cagtccaatc ttatgcttga caatttcttt tactgcttca
    39301 tttgctggaa tcacattatg gcgaagagac ttgttgattt ctgtttcact aaaggtttct
    39361 ttagctactt tcgtccagtt gactaaaagc tccgttccaa cattgaattt gcggatcccg
    39421 ttattgatca attcaggata atcttcttct ttcacacctg ttcctccatg gataactagg
    39481 gggacatcaa ctactgcatc gatttctttc agcaagggaa tgtttacttc tgtttttgat
    39541 ttgaattgtc catgattcgt accgatagca atagctaatg catccacgcc tgtagcttct
    39601 acaaatttca ctgcgtcttc cggtcttgta tataccttgt cattttcagc gacatggatt
    39661 ccttcttctg taccgccgat tgttccaagt tctccttcta cggaaacacc gtgcgcatgg
    39721 gcatattcta ctactgcttt tgtcttcagg atattttctt tgaaaggcag atgggaccca
    39781 tcatacatga cagaagtata tccgctgtcg attgcttctt tgatatcatc aaagtctcgg
    39841 gcgtggtcta agtgcaagac tgcatcaacc atttcttctt ctgcaatagc tgtcattaca
    39901 ttgactaaca catgcatgct gatatattta gcagtatcga cacttgtttg aataatgatt
    39961 ggcgaaccca tttcttttgc tgcgcctagc atggcaggaa gcatttctag gttatgcgta
    40021 ttaaaagctc caactgtaaa attcaactct tctgctgttt ttgttacttc tttcaatgtt
    40081 gtatacatct tttattgcca ccttatcttt ttatttgttt cctttgatcg ttacttgttt
    40141 tgcaatttgt cgcatctcat ccattttgcc catgtagtaa tcgatccctt gatcatcacc
    40201 agcacgagca gcatctgaac gcttctggtt atagaggctc atcagttttt tatacccatc
    40261 tccgatcgtc tgtttatttt ccaagctctt ttctaaatat tggatggctt gatcgttctc
    40321 atacaattca gaaaatttaa gtccaacttg ttcataaagt tccgctaatt tatgcctatc
    40381 ttgggtagtg gaaatctctt gtttcaattg tgtaatctct gattgaatct tttcggtcgc
    40441 ttctggcgat agggcttttt cttcttttgc cagaactttt ttcggttctt tttctttttt
    40501 cttaaaaaat ccaaacatca ttttccctac tttcaattaa aatgctttca aaactgggct
    40561 taagatccag gcatataaga gtgctgcggc tactgtatct acatcggttg ctgtagcatt
    40621 gatgaatccc atcgaattaa agatcgttac tagcaaagcg ggcaataatg taataaagaa
    40681 accatgagca ataccgccaa tgatcgctcc tcggcgtccg ccaactgcgt tgccaaatat
    40741 ccctgctgtc cctccggcaa agaaattggt tagcatacca gataaaatca tcgctaagcc
    40801 gaacatgggc aaagtaaaca tggcaataat cgtaccaatc gttgtcgtaa tgaaacctaa
    40861 aatgacagca tttgggctat aaggaaagaa aactggacaa tctaaggcag ggatcgcatc
    40921 aggaactaat ttcatagcta tccctcgaaa tgccggcacg atttcaccta acagtagacg
    40981 tacaccagct agcaatacat acacacccac tacgaattgg atggcttgta aaaaggcaaa
    41041 catgatatag ttttgatctc ctgataattc agaagcgaat ggttcgcctg caaataaaac
    41101 agtgatgata tatagcggga ccatcacgac catgacagac aaataagtat cttgcagaaa
    41161 ttcgaaatgt gtcggcaact tgatatcttc gatcgacttt ttattttcgc ctttctcacc
    41221 aaaaagctta gcgacaccgg cttcaaataa gtacccgatc gtacaaaaat gacctaaagc
    41281 aatatcattt gatccagtga ttttacgaat gatcggttgc gctactgccg gcatagcaat
    41341 ggcgaaacaa gctccgataa aaccaccgac caagatcaat acgatccctc gcagaccggc
    41401 aaaatagcca aagactgtcg tcatcgttgc catccataaa attgcttgac cattgataga
    41461 ttcaatagaa gggatgatcc cttgcatatg aaatccttca gtaaagattt tcccgaaata
    41521 agttaaactt ccaacgatga tgctcgaacc ggcactcaac acttgaaatc ctaggagtgt
    41581 ctttaacgtg ccagagatga cttgtcctgc tgatttcttc tgcagtagta gacccaacat
    41641 agcaatcaat gcaatggtga tcgatgcctg agtcaaaata ttttcgatga taaaattgat
    41701 aacgctcatg tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga
    41761 actggggtga ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc
    41821 gccgttttag gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg
    41881 atcacatctg gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct
    41941 actcctaatt tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa
    42001 cctgcgccgc atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt
    42061 tattatttct caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca
    42121 atcaatcgat ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc
    42181 agatgcgagt aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca
    42241 ggattaccga aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc
    42301 ccatcttctg gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg
    42361 taatcattca ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta
    42421 accaatggtc tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc
    42481 atgatattgc tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt
    42541 tctaaattgt ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg
    42601 ccaccacttt ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg
    42661 ttaggctcta accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata
    42721 ttcgtctcag ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga
    42781 aaggtgctga aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt
    42841 aaaactgcag taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt
    42901 ccagtagcca tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc
    42961 ttgattacac tggcgatcgt caaaaagtgg atgacaagaa aagctatctc gtcctccagt
    43021 atcttgccac caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt
    43081 gggaaataat ttgttcggat gttttctttc aatggattca tgacttggat atgattctta
    43141 caatcagtgc agccatgtgc ttatagagat tttcgaataa agcttcttcc tttagatgga
    43201 aaggtacgtg tgtctcgttc tctacaaatt ggatcaattg gatcgttagt aattgagctt
    43261 gtacccactc gattgaattg gagatatctc tagcattgaa cgtctcaata gtgaaacgaa
    43321 gataatttct ttctacttgc gggacattgt ccaattgaaa ttcctcgatc atgtggtcaa
    43381 taaatgcaga aaagctactg tcttttattt ctgtattttt ccaattaatg gcagagataa
    43441 attcatgttt cctgattcgc tggatccaga tcaatgtaaa aagcaggatt tgttttttat
    43501 aaatatcatc ttcttgtttc aacaacttat tggaatagat ctcctgtatt tttttgattt
    43561 ccaccaaagg aaaataatgg agaaagattt tttgagccgg tgttaattgt gcatcttgat
    43621 tcataaagaa gtcggtcatt cccagattct gattgataaa atcatagatc atcgtacgga
    43681 tagatcgttc tgaaccttta taaaccagtc cttgctttcc ataactgacg atctctatcc
    43741 cataactgac cagtgttgct cgtaaccgtc tcatatcttc atctaatgta cttttagaga
    43801 tttgaaattc ttcttctttt ttgtttagat ggatcgggat tcggctgtaa gcaacggcta
    43861 aaactagatc aaaaatacgt tcgcgccgat tcaaaaagcc gtctttcaca tcttttattt
    43921 cagtttctaa tcgagcttgc ttttcctttt ccagcttcaa caaaaaacct ttgttgcgaa
    43981 ttgtacggat ctccggaagc tttttactgc tcaaccagat attcgcttca agaacttcct
    44041 gacggatcgt ccgctgactg acattgaact cacttgctag atgttttgct tttactggat
    44101 attgggctgt catgattctg tgaagtattt caattgttct tgttttcata gaaatgatct
    44161 ccttctgatt tcaagtatat ggttccgctt acataataaa aagtagatat ttctgccact
    44221 tgaagtggaa agaaagtaag caaagcaaac aaaaaataag ccatatgctt tttcatctcc
    44281 tcaacgaaaa agacatacgc ctattattac aaaaattttg cccatccata caaaaaagag
    44341 gttgtgaaaa agctgtcctg catcaagtaa taagaacaac taaactaaaa tagtgcttcc
    44401 tattttttgt ttggttgttc ttattatcgc agatgcaagc ttttgaacac catttagtgg
    44461 agaagttgtg acagagatgt tcagccttga gcgataagaa gcgattttga aaaatagctt
    44521 cccatatttt acaaaatcgt gccttattgc cgataggcta tctctggcac accgtttatt
    44581 cagaaaaagg agttgcgaca aggcttttgt cacaactccg ttcataaata tttcctgtca
    44641 atccagcata atcggctgaa taaaatcggc ttgccatctt ttttctttct cagaaaaatc
    44701 aaaataccat atctgagaaa aatactgtcg ttgtgtctta cttcctgaga tcaatcgccc
    44761 atcttttcga tcgactgaat aatcacagca tctgaaatca attcggatgg caaaactatt
    44821 ttcgtggatc tgtcgataat ttcctacttc ctgaatagtt atttttttag gtatgatttc
    44881 ttacaccgtc tgctttgttt ttttgcagaa ctttctgatt ttcggcaaac agcttgtctg
    44941 tataacagct tctggctttt tcaggagttt cttcttccca agcggattgg atcttcatga
    45001 atatcttttg gatttccaga aactgtgctt tcttttcttt agccgttcct gacatgcggg
    45061 acaatatctc cgctttcagc cttttctttt gtcttttatt cttgacgata gaaaaagcag
    45121 aaacccctcc tactgacaaa acagctaatg caagaaatcc cgtttgctca taactagaag
    45181 gacctgtact tggaccataa tacccataag aatctctgcc gtaagaacga ctgccaaaat
    45241 aactgccccc atatgaacga ccgccaaatg aacctctgcc cgaacttcga ccgctagatg
    45301 tacctccacc atgaccaccc gcgacagcga atccttgggt cggcagagca aacaaacata
    45361 ggcccactaa caaaatgatt gctaaataat tgattttttt cattgtcttc ctcgttatct
    45421 tttattacaa aaaactattt ttatcatagc cttgctaaaa ggaagagtca ataaccagat
    45481 gcataaactg tttttttagc aattttgtct tatttagtca ttgagtaaca tcgaacagat
    45541 gtatgtgtcg gcagtccata attcctctat cttcagcatg aacaagcttg cagacaagag
    45601 atctgcgctt cctccaggag agatttggtt ttcctgacaa aagtgcaaca gtgctgtaaa
    45661 tgaacgattt ggtaaagcac tttgactagc agcatgagca agtttttgca tttccactaa
    45721 cgttttctaa tcactgcgat gcaatacgca tgtgtcatct agcgaactca tcaaaactaa
    45781 caacatatgc atttgtttct gtacatatga atcattatct agctccttag ctgcttggat
    45841 tgcaagcctg atatgcggat accctaatat tgcttcttca tatgcaccat ttacgccata
    45901 tttttgcttc accctatgac catgagtgtt ttttttatga cgatattttg tatcaggaaa
    45961 agcagcaagc ttgctgacat catcaagtat tttcgataaa gaaaaaggct gttccatact
    46021 gattttttgt gcaatgacac tgatcaatag ccccattgcc caaatcgctc ctttatgggt
    46081 attgatgcca tttgtagcaa tcagcattgc cttttccgct tttcgcccaa tctcagcaat
    46141 tctttctctt aactcctgat caatagcatg atgccatgct atttgagcca tttcttcaaa
    46201 aaaaggagtt agcgtcttag cagacttaag aaataaagca tagtccatat cggaatgtgc
    46261 acccgcatcc agtgcatcca ctaatccagg ttttggactt aatgttactt ctgcgatcaa
    46321 tgcttcctct accaacgcag cgagttgctg agtcactgct ttttttcgtt cgtcatttat
    46381 catgacactc actccttctt cctactatta cttgctgaaa ccttttttga tagctaccca
    46441 aatgatatga agtaaccagc ttaaagcaaa tgcacaactt gccgtcatta gataagctac
    46501 aagaatcatg actcctgaag gtaactgcag ccaactatct ttaaacaata ataacagtac
    46561 ttggaaaaca aatacattgg ctaaatacgt acgataagca taaacagaca accatttgaa
    46621 caatggcaat tttggcgaat cgtttacaat cattttggaa gcgcctatga agacagcaaa
    46681 gatgatgact aaactgtata aactttggat cgtattcaag taaggcgcat tcgcaaagct
    46741 caagtctcct ggatgtgcga gcaatgaacg gacagaaaag aacgcgataa ctaaggcaac
    46801 aggtaagaaa agataacgta ctctgtccaa atattgataa actttttcat gataaacaaa
    46861 tgcggctacc ccataaatcc cgtaaatcat gaaactagca acaaaccgat ctaacaagta
    46921 ccagctgtca tgataaggcc cagtcaatac ttgtgtttga tagaacagat accatgcgac
    46981 atataaaatc gttgaaccaa tcaatactgg ccatacatat tttttattag caaatacttt
    47041 gtaaccgagc caaacaaaaa acggcatgaa caattggatc tgcagcatca taacggtata
    47101 ccataaatga ggcgctccat cacctgtgac atatttcagt aaaaagcttg cagtatcgta
    47161 tacggagttt gttgttggac tgctggaaat agccaaagat aagccgtcgt ccatagaaga
    47221 tacggaacga ctagttcaaa aaacttttct ttcaaaaatt cccaataaga caaatgctgg
    47281 cttgttttta ccatattata aacaattgca aaaataaaca ttggtgctgt gtatttaacg
    47341 atgacgtaaa aggtaccaat aaaagctgta tttgttgcgc tgtggctgtt tgccaaagca
    47401 aagctcaata tcgtttgcat cataacagcc tttacagctg atactttctg gaagtcactt
    47461 acgttgattt tgattttttt gttttcaaac atctctttct ctctcttttt ctttattacc
    47521 aaagttcttt agttaattta ggtccttcga ctgttttcaa tagaattttt ttctctggct
    47581 ctctcaaata ctcttttagt gaaaaaccgc cttgccgagt cactacttgg gtatcgaatt
    47641 gctcaaaaaa agcaggatgc gattgaatta cctgaattgg caattcaatc ggtgaatttg
    47701 cgtacagtag caaatcaaaa tcactggttt cttttactgc cggaatccca gtcgataatt
    47761 caaatcctaa gctgccgccg attccccatt tacatcctga taatatgttt ctagcagcat
    47821 gaaattgctg ataaacagga taatccatga ttttctcttg tctatacgat tcttgttcga
    47881 tgatgtccca cggatgaatg acttgtttga cggcatgttt tgagatttct gttgcccatc
    47941 tctgattgcg ggcaggtcca cgtaaaccaa tcggtagtat ttctgcgtga tccgtcgacg
    48001 cacgacgaac tgttccgaac atcttttcag ttagaacgac ccattcaggc aaggtgaacc
    48061 cttctatttc atgtttatca aaaaggacaa tatcatgtgc ttgaatcatt ttttatgcat
    48121 cccattcttc gtccattttg gcacggactt tatttgtttc gacacgtccg aatgttttgg
    48181 ctgtttcgtt tgtgtatcga tttgtcagca tcgtgtcatt tgtattgcgg acatcttgaa
    48241 tggcttcatt gattgcttcg atgactttgc tgacattttg ttctgaagca gcttggtcgg
    48301 tgatatcttc taggaaatga tagatcgctc ctagttttgt ataattattg atatcgtaag
    48361 cgatagctgg aactttttca gcagctgctt cgatttcagc aaccgaacgt tttgtcacac
    48421 gtgcagcact ttctttagac atcgcttgga cggtaatttc tttattgttt aaagcaatca
    48481 agcggcttga ttgtaatcca tgtgctaaaa atgcacctga aatagcattt cctgcaatca
    48541 atccaataac tggatatcca gcttgacgga attttgcata agcagcagca ctattagcca
    48601 aagccacatg gataccgatt aattcttcgt tatagccgta tgcttggctt ggtacatcga
    48661 tcacaacgat gattggccgt ttcacttttt tatctttatc ttcttcgtaa acgtgatcca
    48721 aaatttgtcc catgcggaaa ccttcaacta aaccaacttc accgtttcgt acacggtaca
    48781 tcgggttttt aggatcaggt acgattgata tcacgatggt ttcttctatg aatttacttg
    48841 atttaccata atacacagta ctaatagaag aagttggatt tttcatgcca gttagtgccg
    48901 caaaccaatc gtaaccaaca cttgctgcag ctggttcatt tccttctgct acttctggta
    48961 agtcaattga ttttgcttga tggttagcaa ttgcttcatt gtaatcgtca atggccattg
    49021 gttttgaaag atctaaagaa tcaagtaatg ataaataaaa tgctgagctt tcactgcgat
    49081 gcgtgtcttt tcgttcgtcc attgctgcga tgacagcagc tttgattgct tctacactat
    49141 cagtcactaa ttcatcaatg atttttgact ctacccgttg acgagatcct aatgtattcc
    49201 aaataaagtc tttgtcacta gaatcaaatt ctcgaacgcc tgcttcttgt tcaataactt
    49261 ctggtccatt caaaccgact ctcgcttttt ttgttgcgat taaataactc attaatgctg
    49321 aagtaatcga cataccgcca aatgatccta cacgtcctgg aatcaaaccg atgactggta
    49381 catattgttt tagagcaatg attacatttc cgatttctga aatagataac agtccgtagt
    49441 ttgcttcttg caaacgaaca ccacctgtat ctagtacaat gactggaaag atctcatttc
    49501 cttttttatt ttcttctaac acatgttcaa gtgcagcaac gatttttgca ccggaaactt
    49561 caccgatacc gccaccttgg aattttcctt ccatagcgat tactactgct tgtttgccat
    49621 tgatcgtgcc acggctgaca acgatcccgt catcactttc agggacgatt ccttgtgctg
    49681 gcaaacttgg cgcgatgatt tgatcgaatg gatcaatcaa ttcacgtcca ttgtcaagta
    49741 gagcgttcac tcgttctctg gcgtttaatt ctacaaaact atttttcatc tgaaagttcc
    49801 tccattgctt gcgtcaatcg taggtacaca actcctggtg tagcaccgaa gtcattgatc
    49861 acgatatctg ctgaaattgg ataacgatca aaaaatctcg ttaatacatt gttccatact
    49921 tcgccgaagc catcgcttcc tgtacaaatt tttacagtag attctgtttt ttctgttgga
    49981 aacatcaaga tttccaaatc tcctgaaccg accactccta catggatttt cttattgatc
    50041 ggctgtttcg ttggatattt ataagctaat tgttccatgg atctctcccc tttttgtaaa
    50101 acagagcata cacgttctgc ttattcgtct ttcttcttcc atctgggtaa atggcgacaa
    50161 aaggtttttc tcgccaattt tatgaccaag ttctgaattt cattggtggt tcatacaggc
    50221 catctgacca ttcgatcaaa tcatcgattg tttgtgcagc taataatgaa cgtttcgctt
    50281 cactgcgttt gatacctaga tcttctggta atttcacgat gccggcttca cgcaagctat
    50341 ctagtgattc tttcgtgcta gtcattccaa gaggtgtaac accgccaatt gctgcaattg
    50401 ctgcttgacg ttcttctttt gtttttgctt tgtacaaata agcaattcct tcttctgtca
    50461 cgatatgggt agtatcttcg ctatagatca tcactggtgc attggctaag cctgcctgtt
    50521 tttgaacaac taatgcatct aattcttcga caaagactgg ttttttattt gaaccatatg
    50581 tttcaaccat ttgaacaact aatttttgtc ctttacctaa tggatcatca ttgtcgcgaa
    50641 gtgattgcca tgctggtgta gaatgacgac gaccgccagg attatgtccc atgttaggcg
    50701 ctccaccaaa accagataaa cgtccttttg ttacggtaga agaatttcct tcgtaatcta
    50761 attgcaaggt tgaaccgata aacatatcga ttgcatattg tccagctgct tgtgataatg
    50821 ttcggttact gcgcatcgtg ccgtctttac cgacgaaaaa gacatctggg cgagcagcaa
    50881 tatatttttc cataccgact tcaccaccga aactatggat actttcaacc caacctgatt
    50941 cgattgctgg aatcatcgtt ggatgtgggt tcaataccca gttcttcgcg attttccctt
    51001 ttaaccctag tgattcacca taagttggca gtaataattc gatagctgct gtattgaatc
    51061 caataccgtg attcaaagat tgtacgccgt gctctgcgta gattcctttg attgccatca
    51121 ttgccattag gatttgcaat tctgtgatgt tttgaggatc tcgtgtgaat aaagcttcta
    51181 attgataagg ttcatctgct tttacgacaa cgtctaccca gtcaccaggg atatctacac
    51241 gtggtaaaga atcgacaact tcattgactt gaacgatcac gatgccatct ttgaaagctg
    51301 cagcttcgac gattgttggt gtttcttccg tattgaaacc tgtatacaag ttgccgtctt
    51361 tatctgcttt atctgcaact actaagacaa cgtttggtat caaatcgatg aataagcgtc
    51421 cataaagttc caaataagta tggatatcac ctaatttcat tgtgccgtct tcaatcattt
    51481 gggcgacacg caagctttgt gcacctgcat aagagaaatc gatcttgctt gcgattcctt
    51541 tttcaaaaat attcaaatgt tctggtcgtg agatactaga catgatcaaa tgcagatcat
    51601 tcactttttc tgcatctact ttttcaagac tttctgaaag aaaacttgct tgtttttgat
    51661 tgtcgccttc taatacaact ttgtcgccgg gagcaatcag tttttccatt acttctacga
    51721 ttttttcagt ttctacatac ttgccattga gcaagttgct gattttttcc aatcgctgcg
    51781 cttttttttc gcgtttagaa gcccagttgc ggtttggttt ttccataatt agtcatctcc
    51841 atttttgata tagaaacgtg gcgtcatcaa taccatgttg gtctagattg atcgtacgga
    51901 tccctttatc tacaccaaat tttgtatgaa tcagctttgt caaagtattg ccggggggaa
    51961 attcaatcgt cagatccatt cctaattctt ttgccaagtc catcatttgg ttccactgca
    52021 cagggaaagc tagattttcc aacaaatctt tacgaatctc ttctgcgtcc tgagtggctc
    52081 ttccatccgt attttttata tacaaacatc tggcatcaga tagatggaca gactgcatat
    52141 acggcttgaa tttcttgacc gtatcattca tcaagagaca gtgagatggc acagggactt
    52201 tcagcagctt cgctgtttgc gcttggtgcc gtttggcttc tgccaacgta acggataaac
    52261 cttcgatact cccagataac gtatgttgca tcggacaatt ttcatttgac agataaactg
    52321 gatggtcagg atcaaatgtt ttggctacca tttcctctgc ttcgcttctc gtcacaccta
    52381 ctactacgcc cattgcataa ccgcttggat atgcttcttt catcaagact gcgcggtgat
    52441 agactagttc cgctgcattc tcaaatgtca aagatcgaca agcaacagct gcacaaaaag
    52501 caccgatcga atgtcctgct gcaatatcag gctgaatacc atttttttcc atttcatcta
    52561 aataaaatac tgcttttgtc agtaatgcta gctggataaa aaccgtatct tgatagtttt
    52621 cttctttatc gactaaccga taccccgtca cattttctaa acgttccatg tacatttcag
    52681 ggatatccgc taacatccct aacttctgac tgccttgtcc aggaaataag aaacctgtac
    52741 tcatgagcct cgcctcctca gcacttactt tttccactgc tatcttagtc aatttctgct
    52801 ataataaaaa atgccaaaaa aagaaaaaac cataacaaaa aggtatggat gggatggata
    52861 attatgaaat tgcttcatct gcagtatttt aaaaaagtat tcgaaacaaa aaatgttacc
    52921 caagcagcca aagaattatt tatttctcaa cctgccttga gccgtgcgat caaacatttg
    52981 gaaaacgaat taggtgtccc tttgttttac catagcggca gaaacattga gacgaccgtt
    53041 tatgcagaag aattttatcc atatgcagtc aagacattat acacattaga agaaggaatc
    53101 aattattgtc ttcggtcaat gagaagatcg ttacttccgt catattgtat ttagaggtag
    53161 cttctgtttc tattccgaac ttagtccgaa tttttatgga aaagcatcct gatatccaat
    53221 taacgatcat gcaacatcat ttaccagatg atattaccga tcctgtactg tacatcactt
    53281 cggagatgaa gcctggcatg accaatattc ctatcatgaa agaacctgtt tatgtcgcca
    53341 ttcctaagaa acatcctctt gcgaaaaaag aacatctgca attagaagat atccagcgca
    53401 cccccatctt gatgctgtca aagagcaacg ccttccgtca tacactggat gcagcacttg
    53461 aagcgaaaag tatcgaattg acgatcagtt cgacgacaga tgatcctgcg acattacgtt
    53521 ctattgtcaa gcaaggatta gctgttagct ttttcccaaa agtttcttgg tcttacgata
    53581 aaaatgatcc tttcgtcttg cgggaaatca aagatttgcc cttgacacga acaatttatt
    53641 tatcttctag cttcacagaa gatcatcctt tgatcaaaat gattgcaaaa acgatgcggg
    53701 aattttattt aggaaaataa aaaaaaaaga gctgggacaa aattcttgtc gcagctcttt
    53761 tttcgaacaa acggtgttcc agaagtaatt tcttagaaaa taagccgaaa tttcccaaaa
    53821 atttgagaag caattttcgt aaattccttc ttatttcttg aagctaaaca tttctgtcac
    53881 aacctcttct ctattgtcct agacattcat tgcgttgatt taaaacttcc atcaatcaaa
    53941 tctgctagtt catctagctt ttctatttta cgtagatcaa tagagatcca atgttcttta
    54001 ttcatgtgat aggctggaaa atacccctct tttcctcgca aaattccgcc aagttcagga
    54061 tcgaccttca aatcaagaat attgatgatc ctatggtcac ttgcatcatc atagattttt
    54121 tcttctggaa cagacatcag gaccccaaac cattttttgc ttttttgatg tctgaatacg
    54181 caataatcag gaaatttttt aaaaatatat tctggagaca aattgtatgt ttcattgatg
    54241 tgatcgatta gttctttttt cgttagcatc tgcttccatc tctttcttat tgattcgctt
    54301 tattcaaaga taccacgtaa gaatagaaaa atgaatacaa gtagctgcta atcaaatact
    54361 tcgatatttt tcttttttat caactcttaa atgttcaatc cgcctgtgtt ggtcttgcta
    54421 tcatctacta ttaaaatgtt gattattgtt tgcccaccta ttttttgatt ttagtggtat
    54481 catttttcaa gttcatgatg aaattcttat catcagtaaa tgctccagaa aaataaacaa
    54541 tgattgaaaa atcagttgaa acttgaccac cgtttatttt ctccgtattc ttagaataga
    54601 agggagtctg attgatgtat tcactaatga aaaaaatcat taccgaaaaa gatattcgac
    54661 gtcatgtttc tttagtagaa caattgctga atcatacaaa gataaccgta aatgaattag
    54721 ctgaaatcat agggacaacc gaacgaacga tttattctga ccttcaatct attcgctctc
    54781 aactgcctga aggctgggac atcattagcg atcaagcagg gattagtttg caaaaccagc
    54841 aaaatttatt agcaaatgac ctttgggaga tttttttcaa acaatcagtc agtgtcgaac
    54901 tattgaaaag tctgcttttt acaaaaaaag ttgctgtccc tgattttcta gccgaccatg
    54961 ggctttctta tgggacattg aaaagacatg tgactaaaat caatcagcgt ttagcaagtt
    55021 acgacttgca gatcgatttg acgaaatata ccgcttctat tcttgggaaa gagcgtgcga
    55081 ttcgcacttt ctatcatcga ctgctgattc cttttactca taacaactat tttttcgaag
    55141 attattcgat tcatgaatcc cattattttc aatttttaaa aacaataaat cgaacggagc
    55201 tagcggttga aacagaagag atttttggaa catgttggtt ctttatcaat acgatccgta
    55261 taaaagcgaa ttgccgatta gattcttcta tccatatcaa cagtacactc tcctccctgt
    55321 atgactctgc attgaaaaaa ttgtatttaa cggaaggtat ttatctcaaa gacactgagc
    55381 tttcttttgc ttctttctgc tttctggaaa gttggaatta caacaataac tatggacaag
    55441 aaaccgcttg ctgcctgcat cattctccat ttttcgaacg cctcgaaacg tttgtagagg
    55501 aactagcttc agagctgtct cttgatcaac taaaaaaaca tctctaactg ataacttggc
    55561 tcttctcgta ttaaaatatc atgaatcgcc gatattgatt gaagcattgg accgtcaata
    55621 ccattttttt tagaaacata tgatcagcaa tatgcacatt tgtctcaaca gaaagagaat
    55681 ttactgaacg atctaaaaaa agaaatggaa atcaatgaac cgaatttctt ccttcaactc
    55741 ttatctttac tgatccaaca aacgattttt tctatcaaac cgaatttgtt caatgtctat
    55801 tttattttcc aaggagaacc ttcatggaag gcattctttc agcaggaatt aaaagattac
    55861 ctgggaaaac gtgtctgctt cttacctatc gaactaaccg atctttccac tatttctttc
    55921 gaaaaaacag atatcttgat ttccaatttc ccgctggatt ctctagatat ccctatcgta
    55981 tacatttctt ctattcctac gaaaaatgag ctcaatcgtt tgactgaact cactttcaaa
    56041 acttttttat agtaacggct agcgactgta ttctctagct gttttttctt ttttgcgaaa
    56101 cagtttattc atgatccctt tttctatttc tacaactaag aatagaagaa tcacatttgc
    56161 tccaatcaat aactgttgtg tcatcgttaa acttactgta ccgatcagct gctggccgac
    56221 tggtagaaaa ataaccatcc cttgcaataa agcaaggata cctaatgaaa gaaaaagcgc
    56281 tttgttttgc aaaagaccct ggttcaatga cggcttggac aactctctgc aattgatcat
    56341 gtaaacagct tgagctaaga cgatattttg tagaagaatg gtttgctgta agctttgccc
    56401 ttcaaactgc atagccaata aataggcagg aaccatgatc agtagggaga catagatgat
    56461 ccgaaacaaa tgataaccag ataggattcc ttgctttgga tctcttggtg ggcgcttcat
    56521 gatttctgga tctggcgact cgaaccctag tgcataagat aaagtgattg tcgttaccat
    56581 attgacccat aaaatctgta ctgggaacaa aggtagcggg cgattcagca taagcgccca
    56641 aatcaagatc aatccttgag ctaatgcagt tggcaagaag aaattgatgg ttttttgcaa
    56701 attatccaaa atccgccggc cctcttttac ggcttttgca atcgtatgga aattatcatc
    56761 tcctaaaacc atatcagctg cctgtttgct tacttcacta cctttgattc tcatagcaat
    56821 tcctacatcg gatcttttca aagcaggtgc atcatttacg ccgtctccgg tcattccaac
    56881 gatttatcca tttttctgca aagctttaac aattcgcagt ttatgttctg gcgtcgttcg
    56941 agcaaagaca tcgactattg gcacttgctg aatcaactct tcatcagaca taagatccaa
    57001 atcgatctct tctaaaactt tggcagtatg ttttaagcca atttgttccg caatcgcttt
    57061 tgctgtgtct ttgtgatccc ctgtaatcat tttcactgag atccctgctt gttgacattc
    57121 ctttacagct ttgactgcac tttcttttgg cggatcgatc aatccggcga tccctgcaaa
    57181 agttaattgg gttaaagtct catgcgtgag ttcctgtttc gaatctactt ttttataagc
    57241 aaatcctaaa acacgctgac cttttctcgc aaattctgca gctgtctgac tccatgcttc
    57301 ttgttctgca gaagaaagag ttgatttagc aaataatact tctgatgcac ctttgacgta
    57361 aatcaccgct cctttttcct ccgcatgcat cgttgccata tatttgtatt cagaactgaa
    57421 cggaatcttt gcttctattt ttttcaaagg cagattggct gtttctgtgt gtcgaatcag
    57481 cgcaacttct gtcggattac cacttaaatt ttctgttttc tgttcttctt ttgtttttaa
    57541 ttcctgacag tttttcataa tggacaacac gcaggcttct tcttttgcag ctacatccac
    57601 aactgtcatt tcattttttg tcaatgttcc tgtcttatct gaacagatca ctgtcataga
    57661 acctaatgtt tctaccgaag gaagtccttt gatgatcgcc ttttcttttg ccatttcatg
    57721 tactccaacg gataggatca tggtcaaaac agctggcaat ccttcgggta ccattgacac
    57781 caccaaagca atcatagccg aaagaaggat atgccaatcc ataccatatc gaaatgtcgt
    57841 aaaaataact aagaataggc tcaaacaaag aatgccttga aaaatttgtt tgtttaatcg
    57901 gttgattttc ttaatcagag gcgtcgtttg ttgttcgacc tattgcaatg cttgattgat
    57961 tttaccgatt tctgtcgtgt ttcctgtttc tacaacgaca ccgactgcag aaccagattg
    58021 tacaagcgtt cctgaaaaag ccatattttt ctggtcgccg atcgattctt cttctgtcaa
    58081 aacggcatga cttttttcag atgcttctga ttctcccgtc aaaatcgatt cttccactaa
    58141 taaatcatgt gcttcgatta atcggatatc tgctggaacg acatctccgg cacgtaagac
    58201 aatgacatct ccttttacca gttctttcgc agggattttt tcttgcatcc cgtttgatag
    58261 tactatcgct tcttgtccca tcagctgctt caaaccattc aatgattctt ccgcttttct
    58321 ttcttgccag taactaacga gtccattgac gatcactacg aataaaataa tacttccttc
    58381 aatatattca ctagaagcaa atttcaaaaa agaggcaatg atcaacacga tcattagcag
    58441 atcagtaaag tgctttgcta tttttttcca tcgttttgtc tgctgccttg cttttatttc
    58501 attatatcct tgatcagtca ggcgtttctg tcgttcttct tgcgacaagc cttctgttga
    58561 tgtttgatag tttttcaata catcttctac cgtcaatctg taccagttca tcctatcccc
    58621 tcctgtttaa ttgatactgt tattatacgc agggaagttt tttcaggcgt atcgattatt
    58681 tcaggggggt ctgaaataat tcgttttgca gaatgtatct gggcctaact gatatttcta
    58741 ttgcttattc ttttatatac aaaatttaag aatacaaacc agataaccaa taaaaaagga
    58801 ataaagcgtg tcgtgctttc gaataacaag ctgaaagcaa ttgaagtaaa aaacaccaac
    58861 aaagcaagat ctttatatct ttgtgtagtg ttccgtttct tttctttaat aaattgaaca
    58921 tgtgtacaca agatcgaaga ccacacaaat agaaaacaac tcgttgtcat gcctgccaaa
    58981 agctgaaaaa acaatcgact atcagctaca aaaaaatgta gacaaagacc ggcaaaaaat
    59041 aacattcctg taattaaaat accgtgtaaa ggtacgcctt tcttagaaag cgcacaaaac
    59101 caatggggca aacttttctc ttttgctgtt tgatataaaa tcctgctagt actgtagact
    59161 gcactatttg ctgaggacaa agaagcaacc aaaatcatta gattgacaat tgctgctccg
    59221 ccttggcaat ggatcatttg cagcatagtc actaaaggac tttcttttaa atctaaacaa
    59281 tgccatggaa caacaaataa aatcgacaaa atcgttccta tatagaaaaa gagaatacgc
    59341 caaggcagac agttgattgc ttttttcaaa ttcttttgcg gatcacttgt ctcgccaacg
    59401 gttaacccga tcatttcaat tccagcaaaa ctgaataaaa ccataggaaa tgagactacc
    59461 attccagcaa agccaaacgg tagtaattca tcgatctgtc caaaattttc tttgattccg
    59521 atagactggg tatgaaaatc tgagacactc aatcctatac ccaataagat aaaaaggaaa
    59581 atccccagga tcttgatgat tgagaaaaaa gattccaatt caccaaacca cttgactgca
    59641 acaaggttga ctgccattaa gatcattagt gcgctacctc cgcttatcaa agctggtaca
    59701 tgtacaaacc agaaatggat atacaatcct attgctgcta attccgcaag tccagctcca
    59761 agccagcaaa gccaataagt ccaattgaca aaaaatgccc actttttgcc taaataatag
    59821 tcaacgaaat cactgataga gttaaatgta ctatttgcta aaaataaatc acccatggcc
    59881 cgcatcaaac tataaatagc caaaccgcat atcaaataaa cgagaataac agctggccct
    59941 gctgatgcaa ttgcctttgc tgaacctaaa aataatcctg ttccaattgc gccacctaat
    60001 gcgattaact ggatatttcg attagttaga gtccgtttca ttgccggttt ttccatcaag
    60061 ttcacctttt tctttttcaa gcagcatggc gacttcctgt tcccaagaag gtttctttat
    60121 tttttcaata cagagtggta ctacaaacaa aatgacactg atggctgtga ctgcaattag
    60181 attgatccat gttccagctt tcatcgtcag tccgacagaa aatccgattg ccgcaaataa
    60241 aacaataacg acagtccaaa ctccaagatt gctctttttc tctttttttc caattctaaa
    60301 tggtcgattc aactttgcat gtgtataacg caatctcaaa actgcgatcc ccatgatgac
    60361 atagacgata cagtagataa cagtagttga atttacgagc atcaaaaatg cttgattgac
    60421 gccaggaatc agcaaataaa aaagtgcaaa gattgagata atgacagact gagtcaaaat
    60481 aacggatttg gatacgtcaa attgattcgt cttccaaaaa ttgaattttg gtggatacgc
    60541 accttttcta gcactttctg tcactgtctt tgctggtcct gaggcccaag cagataattg
    60601 gactgctaca ccaatgaaga caagtaaact aaacagattc acaataagat gtggcaaacc
    60661 aagaatttgg caataaatcg aaatagattg cgcgatatta ttcaattcca tttgtccttt
    60721 tggtacgaca tttgctacaa ctaaagcatt caaaatattg acgaaaaata taaaaattaa
    60781 cgcggcaaat actccgcgaa tatacgtttt aactggattt ttcaatcgag tgatatacac
    60841 agaggacatt tcgataccag taaaaataaa catgatcggc gcaaaataaa caaatgagga
    60901 tgcagtcgta gtatctggta ttaatttgtc ccatgaaaaa ctgcctaatg ttccggttgg
    60961 tacgatccca actttgatcc acgcagcaaa ccctaataag agcatcatcg tcaaaggcac
    61021 gtacaatcct aaccaaacac cgacttttcc gccgattttt gccatatcaa atttcaaatt
    61081 caaaaaagtg atgatccaat aaacgactaa aataacaact aatgtaaatt tgctgttcaa
    61141 accaagcggc acattatcga taatattacc aaacaacgga gcaagtgctg aagcgaccat
    61201 caccatccca ggaaacattt ggacccataa cagccatgag acaacgaacc cccatttttt
    61261 tcctagagca ttcgataccc aaagttctgg accacctttt ttgggaaaca ttccggcaaa
    61321 ttcaccagag atcaatgaga ttggaaaagc gtacaataat actgcaacca gcatgtaaaa
    61381 aatcatcgtc cagctcgttg ctgctacatc tggaatattt cgtacacttg ctacaagggc
    61441 gcaggtcatt ccgataaagg tcatggtcgt gatttgcttt gatgaaccag tatctttcat
    61501 ctttattcct tctttcaata gcgacatatg tttttgtttt cttttatttt tgttcaagaa
    61561 cagaaatgac aaggtttcat cgattcagta caaaacccag tatcaccgaa acatccattg
    61621 aacgatctta gtgaactggg tttgatacat gaaacgaacg atccaactta tagaacgttt
    61681 tataaaaaag aatatcttgg cgaggaatat atgaaaaaca gggggaaata caattgtttt
    61741 gatgaagcaa ataattgtct gttctccata tgtctcttgc tattctttca cacaaaaatc
    61801 agttctattg gatgatttcg tttagtttct tttccattgc tttggcgatt tcttttgtat
    61861 aaaaattgaa aattcgatca tcattcaaat aaggagtcat gatagccgca cgtagcagag
    61921 tgattttctg ttcttttttc cattcttctt tcgttagacc cattctttca acaaaacgaa
    61981 tcggagaatc accgtaatct tcttgtgtaa atgtagtatg agaagtaata aaccgttcac
    62041 cataaacatc gccatccata taagaagacc gatcgaacat tttttcattt aattcattga
    62101 tagcgttcaa atctgtacag ccttgttctt tgaataccca attgaccata ttaaaatcag
    62161 gatgattcaa tggatagact tcgattgttt ttcctttaac agtaaaagtc atatgatcga
    62221 gaaattctct gaatctttga gctgcctcga ttgaagcccc aatcaattga ccgtatccgg
    62281 tcacattcaa tggcaataca cgatgtgttg tccagactgc ggcggcggtt gctccagctt
    62341 ttgatccttc caaaatataa gctcctaaca tatctggagc ctttactgat ttttcaaaca
    62401 catatggcgc gaaataagaa atgatatttc tcatattttt atgtttgatt acgattcctc
    62461 cagcagcata agggacatag cccatcttat gaggatctat cgtgacagaa tccgcttctg
    62521 aaatagctct gaacccttca tatacctctt tgtcaatcgt tacgcagtgg tggaaaacat
    62581 gatgttcttc aaagaattca gctaaggaag catacggcac aaattctcct gcttcgttta
    62641 gaaacagtga acgcgcatag ccgccatatg cggcatcaac atgtaaatag aaatagatac
    62701 cttcatcttt taacctttct cttaattgga cgattttatc tacactgtct acttgacctt
    62761 cttctgtcgt tcctacgaca gcaaccacac ctagaattgg gattttttgt tcagctaatt
    62821 cacgaattgt tttttccaaa gcattaatat ccattcgata atcctcttga actgggatag
    62881 caaccatctg atctaagccg actccgcaaa tatctaacgc cttcatccaa gaatagtgtt
    62941 ttgtttgagg gaccagccat ttccctaatc tttggatatt ttttccgcta cgggaagaag
    63001 ctgctttgac ctcatccatt tcttcgtccg taaagctttc ggtcatttcc aaaatttctt
    63061 caacagataa attcaaaagt tcccactcag acattttttt cactttttca ggcagcactt
    63121 ccttgacagc taaaggaatc gatttgatgc agcgggcgta ccataatcct tcaagattag
    63181 caatagaacc atctgctgca atgtgtcccc atccgtctgt catattgaat aatgaagcaa
    63241 aatcctgtcc tacttctgct tccatttgcg aagttgccat agaagattcc aatgcaacat
    63301 tatttgggtt ccacagcatc gcatagttgt aagctaataa agcaggcatc aatgtttcag
    63361 cattcatttg tccccagtaa cggcctgctg aatgccatgg gatcgatcct gcgcgtaatc
    63421 gctgactgac ttcatccaag acctctatca tatggtcgcg ggtggctaga taatctggag
    63481 tgagcttatc tccttcgcta attgcaggca tatctgaagg aatataattt tctctccatc
    63541 caagatgttc atctaccatc ttgttcaaca acattttata aactggtcca ttttcagctt
    63601 tgtcgcctat aaagacggcc ttgatatcca tatctttcat ttttcttccc tccatatttt
    63661 gttggtcaat gtctcgcaga tacatgtgtt tatctgtaac acctcttcac tatagcgcca
    63721 aaaatgagaa atttttttta taacatcaaa tccacgtaga tttttacaaa caaaaataaa
    63781 taaagtcgga tttttctgat aattaaacgc aaaaaaacga acattttttc aataaatcag
    63841 tgttctaaaa tagataacct aaaaaaag
//
LOCUS       DAMEET010000003        56375 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.003, whole genome shotgun sequence.
ACCESSION   DAMEET010000003 DAMEET010000000
VERSION     DAMEET010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 56375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.003"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(81..1637)
                     /gene="rny"
                     /locus_tag="PB780_000173"
     CDS             complement(81..1637)
                     /gene="rny"
                     /locus_tag="PB780_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDE0732252.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(2048..3097)
                     /gene="recA"
                     /locus_tag="PB780_000174"
     CDS             complement(2048..3097)
                     /gene="recA"
                     /locus_tag="PB780_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDE0732253.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(3206..4447)
                     /locus_tag="PB780_000175"
     CDS             complement(3206..4447)
                     /locus_tag="PB780_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HDE0732254.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(4687..5265)
                     /gene="pgsA"
                     /locus_tag="PB780_000176"
     CDS             complement(4687..5265)
                     /gene="pgsA"
                     /locus_tag="PB780_000176"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDE0732255.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(5474..6397)
                     /locus_tag="PB780_000177"
     CDS             complement(5474..6397)
                     /locus_tag="PB780_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDE0732256.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(7136..8422)
                     /locus_tag="PB780_000178"
     CDS             complement(7136..8422)
                     /locus_tag="PB780_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDE0732257.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(8415..9683)
                     /locus_tag="PB780_000179"
     CDS             complement(8415..9683)
                     /locus_tag="PB780_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDE0732258.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            9935..10396
                     /gene="mscL"
                     /locus_tag="PB780_000180"
     CDS             9935..10396
                     /gene="mscL"
                     /locus_tag="PB780_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDE0732259.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(10455..10901)
                     /locus_tag="PB780_000181"
     CDS             complement(10455..10901)
                     /locus_tag="PB780_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDE0732260.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(10994..11239)
                     /locus_tag="PB780_000182"
     CDS             complement(10994..11239)
                     /locus_tag="PB780_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HDE0732261.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(11354..11932)
                     /locus_tag="PB780_000183"
     CDS             complement(11354..11932)
                     /locus_tag="PB780_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDE0732262.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(12009..12539)
                     /locus_tag="PB780_000184"
     CDS             complement(12009..12539)
                     /locus_tag="PB780_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDE0732263.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(12543..13403)
                     /gene="rlmB"
                     /locus_tag="PB780_000185"
     CDS             complement(12543..13403)
                     /gene="rlmB"
                     /locus_tag="PB780_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDE0732264.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(13396..13791)
                     /locus_tag="PB780_000186"
     CDS             complement(13396..13791)
                     /locus_tag="PB780_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HDE0732265.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(13788..15200)
                     /locus_tag="PB780_000187"
     CDS             complement(13788..15200)
                     /locus_tag="PB780_000187"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDE0732266.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(15197..15733)
                     /gene="cysE"
                     /locus_tag="PB780_000188"
     CDS             complement(15197..15733)
                     /gene="cysE"
                     /locus_tag="PB780_000188"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDE0732267.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(15819..16058)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(16055..17515)
                     /locus_tag="PB780_000189"
     CDS             complement(16055..17515)
                     /locus_tag="PB780_000189"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDE0732268.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(17685..18842)
                     /locus_tag="PB780_000190"
     CDS             complement(17685..18842)
                     /locus_tag="PB780_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDE0732269.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(18876..20246)
                     /gene="radA"
                     /locus_tag="PB780_000191"
     CDS             complement(18876..20246)
                     /gene="radA"
                     /locus_tag="PB780_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDE0732270.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(20309..20836)
                     /locus_tag="PB780_000192"
     CDS             complement(20309..20836)
                     /locus_tag="PB780_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDE0732271.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            21066..21872
                     /gene="proB"
                     /locus_tag="PB780_000193"
     CDS             21066..21872
                     /gene="proB"
                     /locus_tag="PB780_000193"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDE0732272.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            21869..23116
                     /locus_tag="PB780_000194"
     CDS             21869..23116
                     /locus_tag="PB780_000194"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDE0732273.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            23192..23536
                     /locus_tag="PB780_000195"
     CDS             23192..23536
                     /locus_tag="PB780_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732274.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(23653..23979)
                     /locus_tag="PB780_000196"
     CDS             complement(23653..23979)
                     /locus_tag="PB780_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732275.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(24352..25553)
                     /locus_tag="PB780_000197"
                     /pseudo
     CDS             complement(24352..25553)
                     /locus_tag="PB780_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            25670..26185
                     /locus_tag="PB780_000198"
     CDS             25670..26185
                     /locus_tag="PB780_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDE0732276.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(26384..26830)
                     /locus_tag="PB780_000199"
     CDS             complement(26384..26830)
                     /locus_tag="PB780_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDE0732277.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(26877..28355)
                     /gene="msr(C)"
                     /locus_tag="PB780_000200"
     CDS             complement(26877..28355)
                     /gene="msr(C)"
                     /locus_tag="PB780_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDE0732278.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(28709..29338)
                     /locus_tag="PB780_000201"
     CDS             complement(28709..29338)
                     /locus_tag="PB780_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDE0732279.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(29322..29786)
                     /locus_tag="PB780_000202"
     CDS             complement(29322..29786)
                     /locus_tag="PB780_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDE0732280.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(30273..31541)
                     /locus_tag="PB780_000203"
     CDS             complement(30273..31541)
                     /locus_tag="PB780_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDE0732281.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(31772..32509)
                     /locus_tag="PB780_000204"
     CDS             complement(31772..32509)
                     /locus_tag="PB780_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDE0732282.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(32576..33145)
                     /locus_tag="PB780_000205"
     CDS             complement(32576..33145)
                     /locus_tag="PB780_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDE0732283.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            33273..34145
                     /locus_tag="PB780_000206"
     CDS             33273..34145
                     /locus_tag="PB780_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDE0732284.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            34487..35359
                     /locus_tag="PB780_000207"
     CDS             34487..35359
                     /locus_tag="PB780_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDE0732285.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            35356..37314
                     /locus_tag="PB780_000208"
     CDS             35356..37314
                     /locus_tag="PB780_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDE0732286.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(37565..38416)
                     /locus_tag="PB780_000209"
     CDS             complement(37565..38416)
                     /locus_tag="PB780_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDE0732287.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            38539..39006
                     /locus_tag="PB780_000210"
     CDS             38539..39006
                     /locus_tag="PB780_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDE0732288.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(39269..39751)
                     /locus_tag="PB780_000211"
     CDS             complement(39269..39751)
                     /locus_tag="PB780_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDE0732289.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(39851..40714)
                     /locus_tag="PB780_000212"
     CDS             complement(39851..40714)
                     /locus_tag="PB780_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDE0732290.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            41312..42160
                     /locus_tag="PB780_000213"
     CDS             41312..42160
                     /locus_tag="PB780_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732291.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(42331..42672)
                     /locus_tag="PB780_000214"
     CDS             complement(42331..42672)
                     /locus_tag="PB780_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HDE0732292.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            42758..43465
                     /locus_tag="PB780_000215"
     CDS             42758..43465
                     /locus_tag="PB780_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDE0732293.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            43452..43775
                     /locus_tag="PB780_000216"
     CDS             43452..43775
                     /locus_tag="PB780_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDE0732294.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(43864..44901)
                     /locus_tag="PB780_000217"
     CDS             complement(43864..44901)
                     /locus_tag="PB780_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HDE0732295.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(45348..46331)
                     /locus_tag="PB780_000218"
     CDS             complement(45348..46331)
                     /locus_tag="PB780_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HDE0732296.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(46747..47517)
                     /locus_tag="PB780_000219"
     CDS             complement(46747..47517)
                     /locus_tag="PB780_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDE0732297.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(47510..48445)
                     /locus_tag="PB780_000220"
     CDS             complement(47510..48445)
                     /locus_tag="PB780_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDE0732298.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(49039..50688)
                     /locus_tag="PB780_000221"
     CDS             complement(49039..50688)
                     /locus_tag="PB780_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDE0732299.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(50883..51779)
                     /locus_tag="PB780_000222"
     CDS             complement(50883..51779)
                     /locus_tag="PB780_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDE0732300.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(51772..52452)
                     /locus_tag="PB780_000223"
     CDS             complement(51772..52452)
                     /locus_tag="PB780_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDE0732301.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(52453..53235)
                     /locus_tag="PB780_000224"
     CDS             complement(52453..53235)
                     /locus_tag="PB780_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDE0732302.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(53228..54145)
                     /locus_tag="PB780_000225"
     CDS             complement(53228..54145)
                     /locus_tag="PB780_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDE0732303.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(54148..54432)
                     /locus_tag="PB780_000226"
     CDS             complement(54148..54432)
                     /locus_tag="PB780_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732304.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            complement(55133..55753)
                     /locus_tag="PB780_000227"
     CDS             complement(55133..55753)
                     /locus_tag="PB780_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732305.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
     gene            complement(55834..56244)
                     /locus_tag="PB780_000228"
     CDS             complement(55834..56244)
                     /locus_tag="PB780_000228"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732306.1"
                     /translation="MVTLFYRRDFLFSIYTKYFTLSSNIKTSTKLICKLFKLDKISNI
                     DDVKSKILTRKVSEPVKLTAQDKKNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSG
                     EKVTFKINFITAISKLEYGKQKKRDNNDQYLPKK"
BASE COUNT        16146 a        11994 c        10001 g        18234 t
ORIGIN      
        1 aatgatagca atgttttttc gtgccttaca aaaaaagaag agctgtggct tttgccgcgg
       61 ctcttctttt gtatttttta ttatttagca taatcagttg cacgagtttc ccgaataacg
      121 attactttga tgtgtccagg ataatccagt tcttcttcga ttcgtttacg aatatcacgt
      181 actaaacgaa cagcgtctaa atccgagatt tcttctggct tgaccattac ccgaacttcg
      241 cgtccagctt gaactgcgta acttgattct acaccggcga agctgttcga aatattttcc
      301 agacttctta aacgacggat gtagttttct aatgattcac ttcgcgcacc aggtcttgct
      361 gctgagagcg catctgctgc agctacaagg acagagataa cagaagtagc ttccacatct
      421 ccatgatgag aagcgatcgc gttgattact actgggtttt ctttgtactt ggcagctaat
      481 tccgcaccga tctcaacgtg agatccttca atctcatggt caagtgcttt cccaatatca
      541 tggagcaacc cggcacgttt tgctaattgg atatcttcgc caagctctgc tgctaagata
      601 ccagctaatt ttgcaacctc gaccgaatgc ttcaagacgt tttgtccata actagttctg
      661 aatcgcagac gtccgatgat cttgattaga tctggatgaa gtgtatgagc accgacttca
      721 aacgctgcct cttccccgta ttcgcgaata cgttcatcca tttctttacg agatttctcg
      781 accatttctt cgatacgcgc tggatggatt cgtccatctt gtatcagttt ttccaaggtc
      841 atccgtgcga tttctcttcg gattggatcg aatccggaaa gaacaactgc ttccggtgta
      901 tcatcgatga ttaaatcgat accagtcagc gtctcaagcg ttcgaatatt acgaccttct
      961 cgtccaatga tacgaccttt catttcatca ttcggaaggg taacgactga aacagtcgtt
     1021 tctgaaactt gatctgctgc gcagcgttga atcgctaatg ataataagtt tttcgctttg
     1081 cgatctgctt cttctttggc acgctgttca gattctttta ccattagagt taactcatga
     1141 ttcagctctt cttcagtcga tttcatgatg acatctcgtg cgtcctcttt tgataaacca
     1201 gcaatttttt ctagttcttt ttgttgatct tcaatcattt tttcaacgtc tttttctcgc
     1261 tcttcaatta attgctgttt tgaactaagt gcttcttctt tactttcaag tgagttctcg
     1321 cgtttttcta acgagtcatc tttacgattc agcgtttgtt cgcgttgaat caaacgattc
     1381 tcttgagatt ttaattctaa cttactttct ctcagctcac tttcaacttc agaacggtat
     1441 ttctgatttt cttccttagc ttctaacaat gcttcttttt ttagagtttc tgcttctttt
     1501 tttgcactat cgatgattcc agcagctgat gattgtgcac cagctatttc tttctcgtga
     1561 cgtgactttg cgaccataaa ccctaaacca agaccgacaa ttaaaccgat gatagcgagg
     1621 agaatgttga ataccataaa atccacctcc atactatctt tttcattttt atcatctata
     1681 cagcttctat gataaattat tctaaactcg tcaatatgca tcttcgcata tatgtgtaca
     1741 aaaaacatgc acaactttat tctaatgttt gtaccccgta gtgtcaactt taaataaaca
     1801 aagaaacggc tttctactta ttcattaaac gatggttttt aaaataaatg acactagttt
     1861 catataggta aacctttaaa ttgagaaact tttctattaa attattcttt gtagatgaac
     1921 agatattttt attttatgta cccactcgta taattaaata actcacgatt tctttattca
     1981 ataaaaaaac ggcaggtcaa ggagagtacc tactcttcag accagccgtt tttgtttatc
     2041 ataattttta ttcttctact aaaggcaatt cttcttgtcc tttttcatct tctgggacag
     2101 cgacttcttc accaatacca taagctgctc taactagagc agaaacttct gccatcattt
     2161 caggatgatt agccatatag attttagcgt tttcccgtcc ttgaccaatc cgatcttctt
     2221 tataagaata ccaagcaccg cttttatcaa caatgtcttt ttctactgcc atgtctagaa
     2281 gttctccttc ttgtgagatc cctaaaccat acatgacatc tacttcagcg attttgaaag
     2341 gaggtgctac tttgtttttt acgactttga tctttgtacg gttaccaaca atatctgtac
     2401 cttgtttcaa ttgttctgca cgacgtactt ccaaacggat cgttgcgtag aatttcaatg
     2461 cgcgtcctcc aggagtgatt tctggattcc caaacatcac gcctactttt tcacggattt
     2521 ggttgatgaa aatagcgatt gtttttgttt tgttgatcga accagagagt ttacgcaatg
     2581 cttgagacat caaacgtgct tgtaacccga catgtgagtc acccatttcg ccgtcgattt
     2641 ctgctcgtgg aactaaagca gcaactgaat cgactactac gatatctaca gccccacttg
     2701 atactaaagc atcagcgatc tctagacctt gttctcctgt gtcaggctgt gaaagaagta
     2761 gttcatcgat attcacacct aatttttgtg catattgcgg atctaacgca tgctcagcat
     2821 caatgaaagc ggccgttccg ccattttttt gtacttctgc aatagcgtgt agtgcaactg
     2881 ttgttttacc tgaactttca ggtccatata cttcgatgat acgtccacgg gggtaaccgc
     2941 cgacacctaa agcgacatct aacgcaagag agccacttgg aattgtagag atttgttggt
     3001 cgattttttc gcccaatttc ataatagagc ctttaccata gctcttttca atttttttca
     3061 gtgcagcatc taaggctgct ttacgatcat ctgccaatac gataatcctc cttatattta
     3121 caaatcagag caacatttca ttatccttat catatctttt ttagtgaaaa aaagcaagaa
     3181 aaaaccgaac aaatattcgc atttatcatt ttcttaaaag ctgttttctc aaaagatcaa
     3241 atccttgcat aaccgcatta tatctgatag cagcacgatc tctggcaaag tgatattttt
     3301 ccgaaaccac gccgtcttta gatgaaatag cgatccatac tgttcctgca gattgacctt
     3361 ctaacgtgtc cggtccagcc actccggtga aagctacact gtaatctgtt cttgctatct
     3421 cacgtgcttg aatggccatc tgctccacac attctttgct gactgttcca tattctgcta
     3481 atagctccgg atcaatctcc agaaaaccag cttttgtttg gagcgagtag gtaacgaatc
     3541 cgcctggaaa tacttcagat acaccagcaa tgtcgcctaa tgcggcttgg aaggcgccgg
     3601 ctgtcaaact ttccgctgct gttacagtct gcttgttttc tttcagtagt tccactacga
     3661 cttttgctag cgaattatca tcgccgtatc cataaaaata ttcccctact cgttcttgga
     3721 ttttttcttc taatgctaaa agagcttgat tccctgcctg aacatcattt gttttcactg
     3781 tcagtcgtaa tgtcacttca tttggtttgg catatggtgc gatcgttgga ttgatctgtg
     3841 tctcaatcag atcttttagc tcagtgacca gtcgcgattc accaatccca taaaagcgca
     3901 aaactttaga aattagtttt tcttcagaag gaaaacgttc ctctagtaaa ggacgtactt
     3961 gttccacaaa cattggtttc aactcattag gcggaccggg aagaaggata tacgcatgtg
     4021 tatctgtttg ataaaaagtc cctaaagcaa gccctgtacg atttggtaga ggaaccccgc
     4081 cttcgataat ctgggactgt tgcagattgt ttttggtcat ttttcgatgg gttgtttcaa
     4141 aatatgccaa taattttttg tatccttccg tattttgtat gagagactta ccgaggtgag
     4201 ccgcagttgc ttcttttgtc aggtcatctt ctgtcggtcc taatccgcca cataagagta
     4261 tgagttcact tcttgtttca gctagagcaa tcaactcttc taaacgctgc tgattatctc
     4321 ctacaactgt ttgataataa acttcgatac ctagatcagc tagctgttct gacaagaaag
     4381 tggcattcgt attcacgact tgtcctagta aaagttctgt tccgacagct attatttctg
     4441 ctttcattac ttcccgctcc cttatatagt atacgctgta tttttatatt tttttattta
     4501 aaaagagggc aaggagctgt gacaaaaatc ttgtcacagc tccttgttcc aaataaacag
     4561 tgttctcctg ccaaaacctc ggtcataagt cacaatatac ataaaacatg aaaagctgtt
     4621 ttttgaaatt atcccttgtg actcagagtt agacggcagg ctcacaacct ctccactaaa
     4681 cgatacttac attgatcctt tgaacacacc gctgtttttg atgaagtaat ccacacctga
     4741 atagatagta aagatcagac aagcataaag catgatttga tctactggaa taccgatcag
     4801 gctgaatggg atattattga taaacagcaa gataatagca accatttgtg tagccgtctt
     4861 gacttttccc ggccaagctg cagccatgac ttctccatgt tcgacaagta gtaaacgcaa
     4921 gcctgtgacg gctaattcac gacagacaat gatcgctact acccaaccag gggcctttcc
     4981 ttggtcaacc aacatgataa aagcagtcat aactagcatt ttatctgcca acgggtccgc
     5041 aaacttgcca aaattagtga caaggcctcg cgcacgggca atttttccat ctaaccagtc
     5101 tgtgatacta gctaaagcaa aaataactgc acctactagc tgtgtgacag ccagtgtggt
     5161 gtctcctacg ttaaattgtc cccaatctaa aggtaaacct acaaccaaaa tgaaaatcgg
     5221 gatcatacaa attctaagta cagttaattt atttggtaaa ttcacactat cgactccttt
     5281 cttccctatt ctacgtgact atcagcgaag tttccaccga tattatttac ttttccaaat
     5341 gaatttattc aaaggagtat ttttattgaa attgatcttt ttgaattaac gaagaaggac
     5401 cgcgacacag atttgtcacg atccttcttt tttataccta caagtaaaca gatgcgtaaa
     5461 ctaatttata cgatcattct gcgtattgaa gagtaaggtt cacatttttt ggactctgat
     5521 tgttttcccc tggattaact ggtacggatt ctccattcgc tttgatcgtt gcattgcttg
     5581 ctgcaccaat cgtgatggtc gcatttgttg ctgtttccgg taacacggta ctctgtgttt
     5641 catttgctgc cagtgtccct tgataaacat aggcattgtc tactagcaca cctaaccata
     5701 cacgattgac agcagtaaat tcaagggtta caggcttggt agcgtctgtg atatttactg
     5761 cgatggcact gccagaatct tctcctgtag ctattgccat tttcttttct tcacttgatt
     5821 ttgttgttga ttctactgta gaagactctt tggtctgcgt gctttgctct gaggaagagg
     5881 actcctcgac tgatccttct actttaagcg aagaagctgt gtctccgatt attggatcag
     5941 cttgacgatc ttgccaagtc atatagccca cgacaacgac aatcgccaaa gcaaccaatc
     6001 ctaataaaat gacaggaaga cttgtccaaa agcgacttgg atgcgattct tcctgatgca
     6061 ttgcttttct agaagtactt actgtttcag gctgcggacg ttcagggacc gatcctgtca
     6121 atttgctttt cccatcaaaa acatcgacta aatggtctcc atcttctcct acagcttcag
     6181 cgtattgccg aataaatgcc cgcacgtaaa atttaccagg caattggtca aaatcatcat
     6241 tgtcgattgc ttccaaataa cgtttttgaa tttttgtaat ctgctgtaat tcatctaaag
     6301 atattttttt attcaggcgt gcttgccgta atttttctcc gatggtttca aaagccactc
     6361 gttcatcttc tccagtctgt ttgattctcc gaagcatctg ctgttgcaac gaaagacgtt
     6421 atgtttgggt gctgtgacaa aagtcatgtc acagctcctt ttttccgaat aaacattgtt
     6481 ctcctgccaa ctcctcggtc acaagtcaca atatccaata aatataagaa gctgtttttt
     6541 gaacttgttc cttgtgactc ggagttaaac ggcaggctca cgacctcttt cttaaacggt
     6601 gttcaatcag ctgttcctcg gtattagctc aaaaaccggc aaaatataag gagatatttt
     6661 tccgtatgtt tctctaatac tcaggtcaaa acgctgattt cacaacctct tccttaaacg
     6721 gtgttcaaaa gcttgcatct gcggtaataa gtccaaccaa gttaaaaaca ttaagaagct
     6781 gtttttactt ggctgttctt attacttgat gcagggcagc tttttcacaa cctcttggta
     6841 cacggtgttc cagaagtagc ttcttcggca ataagccgaa atatcacaaa aatttgagaa
     6901 gcaattttcg taaattcctt cttatttctt gaagctgacc acttctgtca caacctcttt
     6961 gattacttcg tttgatttta ttttaatata agttgattaa aagagagcat gatttgtttt
     7021 attttctcac taatttttcc aacacaacta agaataccat aaaagctgat tttggaggag
     7081 gttcctacta caagtctaaa acgaaattta agacttttcc tcataaaata ctggtttatt
     7141 ttggtctcat gtaaaactcg ctgaaggcct cttcacttat aaatgcttcc ccagctgcca
     7201 gtacgtcttt catttggatc gaatcgatga tttcaggaag atcaaataaa gttcgatcgc
     7261 caaataaact ttgcgtaaat tgattggcaa tatattcgat cgaatttaat gattggaaat
     7321 attgtccaag cattttcttc ttcagcaaat cgagatttgt ttctgacact tccggatcat
     7381 cagcgaatcc aagaatgatt tctttgactt tttctgctgc tttctctggt tcgtccgtat
     7441 ctccgctgaa gtccgcaaag tgaaattcac gatccaagct gaattcaaaa ccaaagctgt
     7501 catcgataat tccttgattg tacatcgcta gatagtttcg agaagtattg cctaaaatca
     7561 tttggaacaa cagattgatt gctgttttat agcgtaacag ttctgttcct tcttgcggca
     7621 atgtgtctaa tcctttaata ccaagtacaa atttatctcg tgtaatggct gcttccaatg
     7681 cactttgttt gatgatttct tttgtgtttt cagggaaata acgtacaatt tcttgctttg
     7741 gagggaaatt ttttgcttct tggttttccc gaataagttt catcaatttc tcaggctcca
     7801 tcttaccaac tacaaataaa accatattgc ttggctgata aaacgtccga tagcacgtat
     7861 acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt gcccgcaata tcgatatgca
     7921 atgggtgtgt aggatacaag ttatttagta tgccaaaaaa catccgccag ttagggtcgt
     7981 cttcatacat ttggatttcc tgaccgatga ttcctttttc cttgtttaca gtttcttctg
     8041 tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag attcttttct acctgatccg
     8101 tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa agcattggcg gaagctcctt
     8161 gtttcccaaa taattgaaag acatcaccat cttctttttc aaacaactta tgttctaaaa
     8221 aatgggcaat tccatctgga actttcacct tttctttttc accataggga ataaattcgt
     8281 tatcgatcga accataattc gtactgaaca aaccatatgt cttgtgatag tcatttttag
     8341 gcaacaaata cacggtcaaa ccgtttggca gtacttcatg ataaagtgtt tcattgattt
     8401 gctcatattc tgttttattc atttgcattt cctcctgcta aaaagaagat tgcctgcaat
     8461 tcgatttgct cagctacttg ttgtatctct ttgatcgtta cgctctccat acgtttcaac
     8521 cattcttcat cagataattt tgtttccggc agccagctgt ccaaaaaaga agcttcgatt
     8581 gccgcttgcg gagagtctag agaaagcaga tattggtttc tcagcattgc ttttgtctga
     8641 gacagttcca actctgttat ttcaccattt cgcaaacttt ctaattgctc gtgaatcaga
     8701 tgaagtactt gatttcggtt cttttcatcg ataccggttt ggacactcat gaatcctcgg
     8761 aacgtatcca cgctactaga ggcataataa gctagacttt ctttctctct gacattcatg
     8821 aaaagtttag agtgaggaaa accgccaaat aaaccgttaa agaccattag cgcaaaacgt
     8881 tctggttcat cgtaatacac atttgtctga taagctaggt tcagctttgc ttgaacgatt
     8941 ggctcacgta cttgtcgttc cttcacgata ttcacttgag gttgggtata aaacagatct
     9001 ggataaactg tttcccgcga ctcaaatggt aattgcccaa ctgcttcagc tgctttgtcc
     9061 ggatcaacat cgccgacaac aaaaatgtcg atttgatcat ttgccatcat ttcttgatag
     9121 taggacgcta gcgaactagc agtgatcttg tccaaatctt cgactacgcc caaccctggg
     9181 atcttttgtt ccgggctgtt ttcaaaataa agttcttgca aggcatagga agcaaaagtt
     9241 tgtttatctt ccttcaaact ttccaaatat agacgcagat tgttcttttc caagtcaaac
     9301 gtcaactgat caaactgatg atttttaata tttggataaa ataaaacctc ttttaaaaaa
     9361 tctaccgctt gagaaaaaag gtttggatca ttgacatatt caccattcac aaaactgatc
     9421 cctgcgttta gccaatgtaa atttcctttt tttctaactg ataatccaaa gcttgcgcca
     9481 taaagctctg caagttgcgc acttaacttt gtctgatccg gataattcaa actatttgtt
     9541 tccaacatgc tgctgagaag tgaacgtttc gtgatgactt cttgttgcag acgcgcagta
     9601 aaacggataa aaatccggat cgttttgtat tgttttgttg gtaagacatg aagattcacg
     9661 ccttctgcca attgatacac catatttata tgctcctttt cacagttgat tgcccgctta
     9721 ctcttttaat gggtcaacct gcctttttta cttattcctc tgcttgaaat ttataagttc
     9781 aggcaattcg ccgttttttc taaatgatgt tacactttat agtatagcat agatgttttt
     9841 tatcaaaaga aacagacggc taaaagcgta tattgttttt ttatttttta tttttcgata
     9901 tactaaagac aaataaaaga agggagggtc attcatgatc aaagaattca aagagttcat
     9961 catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt attggtagtg cgtttacaag
    10021 catcgtaaac caagtggtgc aaggcttgat cacaccacta ataggttggt ttgtcgcaat
    10081 gatcacaggt acttctgatt tagaaggagc actttcaatt cttgactggt cgccgacaaa
    10141 aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc attacatttc tgatcactgg
    10201 ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg aagaatttac gctccaaaga
    10261 agagcaagtg gaagtggaag aaactcctac tgcagaagat tatcttagtg acatacgcga
    10321 tcttcttgcc aaacaagtgg cagaagacaa tacacatcca gtagagcaaa caaatctttc
    10381 cgatacttct aaataataaa gaatctaaaa aaaagaaagc cgattttcag tgtcggcttt
    10441 ctttttttag attcttaact tgctacttgt tcctttttca tgtcgatatg tggaatatca
    10501 tcttccaaat acacatctga gattgccttg aatccaaacg actcataaaa agcttgtagg
    10561 tattcttgtg cgccgatcac gattggtttt tctggaaaat ttttctggat catgttaatc
    10621 gtttcttgaa caatctttct tcctaaacca tctttacggt tgtcctgatg aacaaggaca
    10681 cgtccaaaat ggatactctc cgcttcttca tatactcgcg tgtaggcaag gatcttccca
    10741 tcagtatctt gaaaaaacgt atgccaagct tgtccatcaa tgtcatcaac ttcttgataa
    10801 ggacactgtt gttccacaac aaaaactgcg attcgttctt tgacgatcgc gtaaaactct
    10861 gctgttgaca attcttcaaa tttctttacc tgatattcca ctaagcaaac gccccctgct
    10921 tttttattct tagcataccg attcgcacac aaaaaagcaa acgaacctta cgaatctcag
    10981 gctcgtttgc tttttaaact gcttcagtta aaaattcgat ctcaactgtc cgagtaagta
    11041 catctgaata gctgtaagat actcgttcaa aagaattttc ttcaggatcc aaatcaacaa
    11101 cgaatactga aggataggtt tctgttaaaa cgccgcggcg ttctgtttga cgctttcttc
    11161 ctgtctgagc aaccaacatg attgggctgc cgatacgacc ttctaaatct ttcttgattg
    11221 aagctaaagt tgttggcata cagttcacct ctactatcaa ctataccaca tatttttaaa
    11281 attttcaagt ttaccataaa aaaaaaagaa atgcaataaa aataaataag aagctcatta
    11341 agatttttct aaattattcc tcattataaa atttcaagtg tttttttatt ttgagtttta
    11401 ctctcccgta agcgctcatt acttgcttgt gatccattgc taaacattca gccatgtcta
    11461 caaaatcaac gccttgcata taataatgaa aaattttagc ttctattttt gaaaactgga
    11521 tttcgcaatt ttcgattgct tcttttaata aaagatgctg aatcgctaaa ggttcttgca
    11581 atgtgatctc tgctaaaaag tctggtcctt ctgtctctat tttttggtcg attgagactt
    11641 catctttttg atcacgacgc ttcagcgcgt tctgcttcct caacagagaa tagatttgat
    11701 tgcttataat gcttttataa taacttccaa agctaacacc tttgcttttt tcgtaagttc
    11761 tcaatgcctg atataaagcg atccttgctt cttgttccca atcctcagaa acataatttc
    11821 ttaaataata ggtgtttttc aaacgtagta aaagtggaaa atatttttga tacagggcag
    11881 caaatgcttc ttcgtcacca ttacgtgcag cgcgcaataa ctcctcagac ataaaataat
    11941 tcctccataa attcgtttat ggaggaatct tatcatcaat gattcatcga actgattaga
    12001 acgatatgtt atttattttt tctcattaaa tcgtccaatt tttgggacaa tttttttaac
    12061 tgctcttcat tccatggaga attacgtcgg aaatcaataa aatgcatatc tttattttct
    12121 tgggctattg ttttttccgt tttttttatc gtcttatata attcataagc agaagttcgt
    12181 aatgctcctt gggaaaagat catccactgt tctgctaaat cgcttgtcgc cactgtcact
    12241 tgtgtcaaac ggtcattcag ctgaccagcg attctctcga tataactgtc agccgtttcg
    12301 tcttctttcg taaaaacaac atccaactgg tatttcttat aattttgttg gatgcccgga
    12361 actagttggg catcgaagac aacgatgact tccattcctt catatttcgc ataatttgat
    12421 aaacgatgta aaagagcttc tcttgcatcc gccattttgt cctgcttttt aagctggaca
    12481 agttccggcc acgaaccaat catgttgtag ccgtctacaa tcaacagctg ttttttcatg
    12541 gtctactccc cctgacgttg ggaaaaagcc tgatacataa gcaaaccagc agcaacactg
    12601 gcatttaagc tctggacgtg cccggtcatc gggatcgtca gcaattcatc catttctttt
    12661 ttcaatcctg gactcattcc tttgccttca ttaccaatga ctaaagcaat tgatcctttg
    12721 gcattccatt tttggaagtt tgtgccggtc atatctgtgc caaataccca aaatcctgtt
    12781 tctttgagct gttgcacagc ttgtaccaaa ttcgtcacgc gtgcaacagg gacatgttcg
    12841 actgccccag tggaagtctt tgttacgata ggtgtgattc caactgctcg gtgttttggt
    12901 atgatcacac catcgacgcc acttgcgtct gctgtccgta aaatcgaccc aaaattgtgt
    12961 ggatcttcga tgctatccaa aatcaaaaag aaggggtctt cttttttctc ttttgttcgt
    13021 tcaatcagtt ggtcaaggga taaatattca taaggtgtaa tcgcaagaac gactccttga
    13081 tgcacgccgt gatcgctcaa agtttctaat ttttgtttag gaacccattt gactggcaca
    13141 gcttgttcac gtgcgatttc tttcagttct tctacttttt ctcctcgtga atcttcttga
    13201 agaaagagtt tattccctcg cccttgttgt agcgcttcta ctgcggcatg gtgcccaaag
    13261 acgaaattat cttctaatgc ttcttcattt tgatcagaaa ctgactgatt tcgtccagtt
    13321 tgctttttat ttgaatatct attttgtttt ttgtcttcaa atccacgttt cgattgatgt
    13381 ttgtttgttc tttctttacc catttttttc accaactttc tgaatacacc agcggatcaa
    13441 ttcttccatc cgctcggttt gttttgtcaa atgcagatag cccatgagcg cttcgaaccc
    13501 tgttgcgacc cgatacgtcg taacatcagc attttttgcg ctcgtatggc ttttagcatt
    13561 tcttcctctg cgataataaa gttcttcttc ttctgtcaaa agatgttctt ccagcatttg
    13621 ctgaatcaaa aaggcttgtg cattcgcaga aacataattc gttgccgcac gatgcagctt
    13681 attgggtttt gtttctcctt gatcaatcag gtggtcacga acatagattt cgtaaatcgc
    13741 atctccaaca taagccaagg ctaaaccgtt caattgtgtg taatctctca tgcttctctt
    13801 ctccatcggg ttccttgcgg tgtatcttct aataagatcc ctttttcttt caacaaatca
    13861 cgaatttcgt cgcttcggac aaaatcacga ttgcttcttg cttgatttcg ttcttcgatc
    13921 aattgttcaa tctcttcatc taataactct tctgctgtaa ataaaatacc gaagatcgaa
    13981 agccattgcg agaataaagc ttctccttgt gccaagacag cggcagaaac tttcggctgg
    14041 tctagataag tgttcagcca cttagcaagc tcatagacca ctgtgatccc atttgctgca
    14101 ttaaaatcat cgtccatttc ttcgatgaat cgctgttcta aattttctaa caaagcgatt
    14161 ttatccgcat catctgccaa attctctgag gcagtttctt ggcggaattt caaattgtca
    14221 aacgtattct tcagtcgttg taaattggtg gctgcttctt tcatcgtcgt ttcactgtag
    14281 cggatcggac gacggtattg tgtggtcgcc atgaagaaac gcaagacttg tggatcaacg
    14341 tttttgatca tttcatgtac agtgatgaaa ttccctagag atttactcat tttttcatcg
    14401 tcttcgccaa tcgttacata gccattgtgc atccagtaat tagcgaattt tttccctgtt
    14461 ttcgcttcac tttgtgcgat ttcattttca tgatgaggaa attctaaatc ttgcccgcca
    14521 ccatggatat cgatcgtttc tcctaaatgt tttgttgcca taacggaaca ttcaatatgc
    14581 caaccgggac gtcctttgcc ccatggtgaa tcccaagaaa tctcgccagg ttttgcttgt
    14641 ttccataaag cgaaatctaa tgggtcttct ttttgctgct gttcttcccc tgtacgctga
    14701 cttgctccga cttccagttc atcgatggat tggtcactta atttcccata attttcaaat
    14761 ttacgggtac ggtaatacac atcacctgaa gattcataag catagccttt ttcaaccaac
    14821 acttgaataa atgctaaaat atctggcata tgatccatga ctcttggatg cagtgtagca
    14881 ggctcgacat tcaacgcttt cgtatcttct tcaaatgctc ggatgaaacg atcagcaact
    14941 tctggtgctg taatacctaa ttcgtttgct gtacggatga ttttatcgtc tacatccgtg
    15001 aaattcgata cgaaatcgac tttatagcct cgatattcaa aatagcgtcg aattgtatcg
    15061 aaagcaatgg tgctacgagc atttccgata tggatataat tatacacagt cggtccgcag
    15121 acatacatac cgacatgtcc cttttgtata gggatgaatt cttctttttt tctggtcaat
    15181 gtattgtaga tttttatcat tctgatctcc tcactttttc tcctttgatt cgaacgactt
    15241 ttgctggtac tcctaccgct gtagcgtcgt ctggaacatc tttcaacaca acggcacctg
    15301 caccgatttt ggcgtttttt ccgatcgtta ccggtcctaa tatctgcgca ttcgcagaga
    15361 gcatggctcc ttgtttaaca gttgggtgtc ttttacccgt ttcttttcct gtgccgccta
    15421 atgttacgcc atggaataaa atcacatcgt cctcgatttc tgcggtttcg ccaatgacga
    15481 ttcccattcc atggtcgatg aacacgccag ttccaatcgt tgctcctgga tggatctcaa
    15541 caccagttag gaaacgccag aattgagcat tgatcttcgc caataaaaag agccggtgct
    15601 gatataaaaa atgagagaaa cgatgccaaa acaaggcatg aaaaccaggg tatgtcaaaa
    15661 cgatctcagc tgtcgaacgt gcagccggat cgtttctctt tgctgcatca atcgctcggg
    15721 taaaccaact catgactttc cactcctttc gtaagaattt tatgtaggaa atcatcttct
    15781 tataaagaaa agaagcgcct tttaaataag tttccttatc aaaagacgct ttccgcgtgg
    15841 ttccactttt cttcataatc aatgattatc tctgacagct aacgcctgtt atacgttctt
    15901 ggctactttg tttcctataa aacagttttt cctgttttct gtgtttcgcc aagaccactc
    15961 ccagaggcat ttcggcaacc attcattgat accttgcacc acccggtatc tctcttgtaa
    16021 tgatcagctg cttacttttt tctgttcatc gtgtttagtt cagtacttga tttaaatgtg
    16081 ctaatgattt ttcacgacca agcaattcga tcgtttcagg caattctgga ccatgcattt
    16141 gtcctgatac agctacacgg attggcatga aaagattttt tccttttacg ccagtttctt
    16201 tttgaacagc tttgatggca gctttgacag ttggagcatc tactacttcc atttgttcta
    16261 gctgtgcttt gaatgcgtgg agaacagtag gaactgtttc accagcaagt acttcttttg
    16321 ctgcttcatt caatacagga tgttcattga aaaatagcgt agacagatca acgatttctg
    16381 cagcatagct catttgaggt tgatacaggc taacgatctt cttcacccat tcgattgtct
    16441 tttcatctgg gttttctgct acacgtccat cagcgattag ataaggcaga cacatgtcag
    16501 taaatgcatc tagatccatt gctttgatgt aatggttgtt tacccattgc agttttttcg
    16561 catcgaaagc tgcgggtgat ttacttaggc gttttggatc gaacattttg atcaattctt
    16621 ggtgttcgaa gatttcgtct tcgcccacag gtgaccagcc caataatgca ataaagttga
    16681 acattgcatc tggtagatag cctaattcgc gatattgttc gataaattgc aagattgatt
    16741 cgtcacgttt gcttagtttt ttacctgttt cactgttgat gatcaatgtc atatgtccga
    16801 aggttggtgg tgtccagcca aatgcttcat agatcatcag ttgttttggt gtattggcaa
    16861 tatggtcatc tccacgtaaa acgtgagtga ttttcatcaa atgatcatcg accgcaacag
    16921 caaaattata agttggcata ccatcgcgtt tttgtatgac aaagtcgcca ccgatattat
    16981 cggattcgaa cgaaatagcg cctttcacca tatctgtaaa tgcgtattct gtattgcgag
    17041 gcacacggaa acgtacaaca gattctagac cttgtgcttc tttttctgct tgttcttcag
    17101 gtgtcaagtt cgcacatgtt cctgcgtaat gaggcatttc tcctcttgcg cgttgtgctt
    17161 cccgttctgc ttctaattct tcttctgtgc aatagcattt gtatgctcga ttgctagcaa
    17221 gcagctgatc aatcagcggt tgatagattt ctttacgttc agattgacgg taagggccgt
    17281 attctccagg attttcagga gattcatccc aattcattcc caaccaagca aggttttcca
    17341 actggctttt ttcgccatct tcgatattac gtttttggtc agtatcctca atacgaataa
    17401 taaagtctcc atcattgtgg cgggcaaaca aataattgaa taatgcagtt cttgcatttc
    17461 caatatgcag gtgcccagtt ggacttggtg catagcgtac gcgtactttc gtcattttct
    17521 tcctcttctt tcaaactaga ctttgactaa cagcaaaaat tccgtcagtt acggcttctc
    17581 aaatatcatt tatttaaagc cactcagcac aaattttacc ttttctaagg taaatagtaa
    17641 agccaactgg ttgaatttcc ttaccagttg gctgaatttc ttttttattt tgattgttta
    17701 gtctttttct ctgtctcttt tttattgtcg attcctcgtc ctgcatgtgc aggtttagca
    17761 aagatcatgc gtccagcagc tgtttgaagg gcacttgtta caacgacttc gatacgttca
    17821 ttcatgtaat gctgtccatc ttctacgaca accatcgtgc cgtcatcaag gtaggctact
    17881 ccttgttggc gttctgtccc ggcttttact actagaacat tcatcgtttc accagggatg
    17941 acgactggtt ttacggcatt tgccaacgca ttgatattaa gaacaggtac attttggaat
    18001 tcagagactt tattcaagtt atagtcattg gttacaatga caccatctaa aagcttagcc
    18061 aatttgatca atttgctgtc tacttcactg atatcttcaa agtctccatc gtacatttcg
    18121 acagatatac cctcttcttt ttgcaaggca tttaggatat ctaatccgcg acgaccacgc
    18181 acacgtttca aactatcccc tgagtccgcg atgtattgta gttcgtaaag aacaaaatta
    18241 gggatcatca aggttccttc taagaatcca gtctttgtaa tatcataaat ccgaccgtca
    18301 atgatcacgc ttgtatccaa gattttatat ttatggaaat tttctcctga ttttcgttct
    18361 aacagttgac tctcaacttg actagctgtt tcttgctcga tttttttgct tcgtgaacca
    18421 aaaactttct tccattcttc gattcgtgtc gttcccattc tcaatcccag atagcctaag
    18481 atgatcatca gtaaaatcgg caatacacta ttgacgaatg gaatttccat attgtaaaga
    18541 ggggtcgaga tcacaacacc taaagtcaat ccgatgatcg caccgatcgc accgaagaga
    18601 agataagtca agctgacctc acttagtttt tgttctactt gtttgataat agctgagata
    18661 tgtttggcca acagcaaaga taataaaaag aaaataagtg cgcctaacag gctgttggta
    18721 aatgtattat tcagccatcc gtttgactgt tgaccgattg cctgccaagc tgagggtaaa
    18781 aatgaaatac ccaagcttgc gccgacaatg atcatcagca aagtaatgac acgtttttgc
    18841 atcgtttttc ctccgtttcg acaatcattt attagttagc ggaacacacg cttcaacgtt
    18901 tcagccaaag ttgctactcc aactatctcg attccttttg gcgcttccca gttacctaag
    18961 ttgtttttag gtaggtatac ttttgtgaag ccgagttttt ggacttcttt gactcgttgt
    19021 tcaatatgac tgacacgtct gatttctcca gtcagtccaa tttcaccgat gaaacattcc
    19081 gttggctgtg ttcctttttc tttatagctt gaagcaatac tgacagcgat agctaaatca
    19141 atcgctggtt catttaattt taccccacct gcagctttta agtaagcatc ttgattctga
    19201 agaagcaacc cagctctttt ttccagcact gccatgatca gagatacccg attgaaatca
    19261 agacctgttg ttgttcgttt ggcatttccg aacatagtag gtgtgactaa tgcttggatc
    19321 tctaccaaaa ttggacgtgt cccttccatt gccacaacga tagcagagcc agttgcaccg
    19381 tctaggcgtt cttctaagaa aatctgtgaa ggattagcga cttcttccaa tccatgctca
    19441 tgcatttcaa aaatcccaat ctcattcgtt gagccaaatc ggttttttac tgctcgcaaa
    19501 atccgaaaac tatgatgttg ttcaccttca aaatataaca ccgtatccac catatgttct
    19561 aacattcttg ggccggcgat cgagccttct ttggtaacat gaccgacgat aaaaatggct
    19621 atcccatttg ttttagcgat tttgagtagt tctgcagtgg tttctcttac ttggctgaca
    19681 cttcctgcca cacttgtgat gtctggctgc gtcatcgttt ggatggaatc aatgataaca
    19741 taatcagggg agatatgttc gatggcacga ctgatttcgt tcatatctgt ttcagcgtat
    19801 agataaaatt ctgtgttgat acttccgagg cgttccgcgc gtaattttat ctgctctgcg
    19861 ctttcttcgc cggatacata taagactttt ccgcctatgg ctgctaattg ctgagaaact
    19921 tgtaacagca aagtggattt cccgatacct ggatcgccac caatcaagac catagaccct
    19981 ggcaccacac ctccgcctaa cacacggttc agttcttcta attttgtttt gatccgaggt
    20041 tcttttttag ggacgacgtc tgcgattttt gttggttttg ctttttctcc tgtcaaactt
    20101 gtccgagttc tgcggtccgt tgtatcttgt tcgatttctt cgaccatcgt attccatttt
    20161 ccgcaatttg ggcagcgtcc caagaactta ggcgacacat acccgcaatt ttggcaaaca
    20221 aattgtactt ttgatttttt tgccattgta ccgcctcctt ttatcctaat catgagacat
    20281 taaatcaagc ttaagtatct ttaacgtttt atttactgga agaaccaaat ccgccttgac
    20341 gttgagcagt cgtttgatct tgatctgctt ttaaaaacgg caaaaagatc ccttggccga
    20401 tacgctcgcc ttttttgatc actacatcct tgaaaccaaa gttggtgaat tggaacatga
    20461 tatggccttc atttgcctca ttattgtaat aatcactgtc aatcacacct actccgtttg
    20521 tcaacactaa aaaccgcttc aacggattgc ttgaacggtt agcaagctgc aaaaattcat
    20581 cttctcccat gtaagctttg attccagtag gcacgagggt tggtttcaat tgtttttcat
    20641 tttctgatac atcttttttc aatccatctg cgactgtttc caaccccttt ttccagcctg
    20701 ctttccagat acttggaatc acgacctctt ctgctgcttc aaaatcatag ccagcagcat
    20761 gatgtgtagc gcgctgcggt agattgatat tttgatcttt atatttagaa ataacttcaa
    20821 atcctctttt tctcaccggt tttcctccct aaataaacta ctcctattct aacatatccg
    20881 aggctgtaaa agactttctt tacaaattct tcaagaaatt ctgctatctt aaagtaaacc
    20941 attaattcgt tctttttcca aacgactgca agttgtacaa aaaaactatt ttttacatga
    21001 ctttactcgt caaaacaagt agaatagaac gaaattaatg agtggatatt tgattgaggt
    21061 gtcacatgcg taaagaaatt aaaaatgcaa aacgaattat tgtcaaagta ggaacaagta
    21121 cattgattta tcccaatgga aatatcaatc ttgcggcaat cgatcagtta gcttttgtcc
    21181 tttctgatct tagaaatcgg ggaaaggaag tgatcttagt ctcatcagga gctgtaggcg
    21241 ttggtatgca taaattacat ttgtcagaac gtccgacttt gattcctgaa cagcaagctg
    21301 cggcggctgt tggtcaagcc gaactgatga atatctataa tcaaagattc agtgtatata
    21361 atcaacagac ggcacagatc ctcgtgacaa gagacgtggt agattatcct gaaagtcgta
    21421 aaaatgtgag gaatacattg gaacagcttt tgattatgga cacgatccct atcgtgaacg
    21481 aaaacgatac tgtagcagtg gatgaattag atcatctgac aaaatttggc gataatgatc
    21541 agctgtcagc catcatggca aaacttgtcg cggcagacct tttgatcgtc ctttctgata
    21601 tcgacgggtt ttattcagat aatccgacta caaatcccga ggctcagatg tatcgctata
    21661 tcgataaaat cgatgaaaag attctggcac aagcaggtgg tgctggtagc acatttggaa
    21721 caggcggtat gcagagtaaa ttaaaagccg cttctaggat atttgaagca aacagcgcaa
    21781 tgattttagc aaatggaaaa aatccggcaa ttatttttga tattcttgca ggaaaagaag
    21841 taggtacttt atttaaggag gacttgaaat gactgacttg atgcaattag gacaacaggc
    21901 aaaagatagc gcgcaacaac tagcacttat gcctacaaac cgcaaaaatg agctacttgt
    21961 tcaaatggca caaaccatca aaaagaatca acaagcgatc attgaagcaa atcataaaga
    22021 cttagaaaaa gcagtcgaaa acaatatcag tgaaacgatg caagaccgct tgcagctgac
    22081 acctgagcga atcaatgcca tggctacaga gatcgaaaaa attgcttcgt tagacgatcc
    22141 tattggaaaa gtagatgaga tgtggacgaa caccgacgga ttgagaatcg gcaaaaagcg
    22201 cgtgccacta ggcgtgaccg gcatcattta cgaatctcgt ccaaatgtca cgacagatgc
    22261 agctagtcta gcattcaaat cagggaatgc cgttatcctt cgcggtggca aagaagcatt
    22321 cttctcgaat caattattag tccaattatt gcagcaagta ctggtcagtg ctggcgaatc
    22381 tccttatgcc atccagtttg tcgatgatac ttctcatgaa actgcaggac agttgatgcg
    22441 attgacagaa tacttagatg tgctgattcc tcgaggcgga gcaaaattaa tccaacgagt
    22501 caaagaacag gcaacggtac ctatcattga aacaggtaca ggaaataatc acgtatatat
    22561 cgataaagaa gcacagttat ctatggcagt gaacatcatc gtcaatgcaa aagcttcccg
    22621 tccatctgtc tgtaacgcgg cagaaacatt gctgatccat tctgagatcg cacctttttt
    22681 cttgccagct attgaaaaag aactggtaga acacggggtc tctttacgtg cggatgctcg
    22741 tgcgttagaa tatctagaga cggctgcttt agcagaagac acagattggg atactgaata
    22801 tctagattat atccttgctg tcaaagtggt cgattctttg gatgaagcaa ttgcacatat
    22861 caatcggcat aatactaaac attcagaaac gatcgtgaca gatagttatg cggctagtca
    22921 acgtttctta aatgaagtgg acgcggcggc tgtctatgtc aatgcctcca ctcgttttac
    22981 agacggatcg gtatttggtt ttggtgcaga gattggtatc tctactcaaa agttgcatgc
    23041 ccgtggtcca atggggttaa atgaattaac ttccacaaaa tatatcattt atggcgatgg
    23101 acaaatccgc gaataatgtt tcgtgtgaaa cattattccc gccttttgcc ttttttctgt
    23161 aaaggttcta tccaactaga aaggtgtcaa tatgtttgaa attttaatga ttttttttat
    23221 ttaccttatt tctctgaata tcgctgcttt tctgggagtc ggtattttat cccttttctt
    23281 tcaattcaaa aaacgaagca tagggagtca acgggaaaaa tggtcgcagt attttgacaa
    23341 aatcgggcaa aaagggctgg ttacacgctt gcacattagt tacatggttg cattatgctt
    23401 attagcgaca atcaattact attctttttt cgatcattca atcgcttata cgattacgct
    23461 actgattgct gggatttttc acttatctta taaatatcag ctgaataaaa accacttgaa
    23521 tcggactttc cgctaaaaaa tatccgtacg ataagtagag tgccttgaag atcggcaaaa
    23581 aactgccgga ctttttagag gctgtgacaa agattttgtc acagcctctt tttccatata
    23641 aacggcgttc gcctaccagc tgcctggtaa atctgtttcc tcttcgttaa gaagcgaaaa
    23701 tccgtcaaat gtttcgccat cataataacg aagcagctca tccatggaca agttcatcgt
    23761 tttttctttt tcttcttccg ctgattcagc cagtagctgt tcccgataca cctgatacat
    23821 accagctatt ttttgctgca tcgcttttct agtttcacct tccgcattga ttattggcaa
    23881 ttctttgata tagccacgat aaatcaaccg atcatctgaa aattcaatgg gtaattcaac
    23941 gagtgtcaac ccgacttcct gaaaatattc ttccagcatc tattatcccc ccattctttt
    24001 tcttaaatga attttagctt acctactgaa aattttccag tttcattatt ttgagaaaaa
    24061 aagggaatgt gacaaaaatc gtgtcacatc ccctttttcc gaataaatgg tgttcttctg
    24121 ccaactcctc aatcataagt cacaatattc aacaaacatg agaaactgtt ttttgaaatt
    24181 gttccttgtg attcagagtt aaacgacagt ctcacaacct cttggtaaac ggcgttcaaa
    24241 agcttgcatc tgcggtaata agcccaacca aacgaaaaat atgagaagct atttttgttt
    24301 ggctgttctt atgacttgat gcaggacagc tttttcacaa cctctgattt cttattcttc
    24361 ttcatttgta gcaaaataat tacggtgata aatgatacaa catacactga taagaacaat
    24421 ggcacctgca aaataaaacg catagctttc accaatatgt tcaccaatca cacctgcaat
    24481 aaatggtgct aaagctccac cgaaaaagcg gacaaaacta tagctggacg aagcaacgtt
    24541 tcgttgcaga cctggtattt ccatagcaat cgtcgtcaac agtgtattga taattccttg
    24601 gaaaaatcct gctaacacaa tgctgatcgg tacaaggact ggcatagaag ttccagctcc
    24661 taataacagc aaacaaataa tgaataagac aaatgctgct aaaattgtcc gataagtcgt
    24721 aaacttccgc tcaagagccg gtgcaacgaa aatcgaagca atagccagaa gcacacccca
    24781 gccaaaaaat acaaaaccga cttctaattc agaatagcca atcaataaaa atggcgcata
    24841 cgcaagtaaa gtaaagaatc cgaaattata taacaatgcg attagtccaa ctgaacgaag
    24901 tttatgattg cttaacgctt tgacaccttc ccaaaggcct acttttctct ttggtttctc
    24961 agtttcaggt aaaagtaccg ctagggctat aaaaccgcaa aacatcaaaa atgctacccc
    25021 aaaaaatgga taacgccatg aaccactgcc caagacccca ccgactagag ggccaacagc
    25081 cattccgcag ccaagtgctg cttcatacat catgattgct ttttctgtgt taccaactaa
    25141 aatactgacg atcgttgcta aagcagtaga gacaaataaa gcattcccga caccccagcc
    25201 ggcacgtaac ccaatcagca ttccgatatt cccagataat ccgccaagac cagcgaatac
    25261 aacgatgata aacaatcctg tcatcaatgt tttttcaaac cgatccgact agataagaaa
    25321 cctgtaaata gcatcaccac accagtgact aacatgtaac tagtaaataa cagggtcgtt
    25381 tcagctggcg tagcgtttaa ctgtgcagca atcgttttta agattgggtc tactagaccc
    25441 actcccataa aagcgatcat acaggtagct gctacaacta gcgctgaact tttccccttt
    25501 gtttcttcat ttgtttgttc ttgtatatct atttgattct ccaatatttc catccctttc
    25561 tctctattag catcatacat gatttatttt acatgtaaat atgcacatgt aaaacgtgga
    25621 aactttactg tgatataata aagaaaataa gacgagaggt aggattttta tgaatacttt
    25681 agcctatata ttattatgcg tgcttttatc caaaccacgt tctgggtatg aattgaagca
    25741 gctgatcaca attttttggg aagcccatca cagtcagatc tatacaacgc ttgccaagtt
    25801 agaccagcag ggttatgtga ccgttttgga caaagacgag cactctcaaa aaaagatcta
    25861 ccacttgact gatgaaggac gtgctcttgt agaggaatgg atcaaagagg aaactcccgc
    25921 tcctacgcaa aaagatgaat ttctagcaaa aatctatgct ttttcgacgc tcgataaaac
    25981 aacagctact ggactgctat tcactagaga acaacagctg aacaaaatca tgcaaaaaaa
    26041 tcaaaaaaaa ttgaatgacc tctcgctaga caagaaacaa gaatttggac gttatgttgt
    26101 gatccagcgc agaattatgc tttgtcagca ggaactccaa tggtgtcagc aagtaagaga
    26161 acaaatgcag cagttttttg agtaagggat aaacagaacc tttctagaaa tatctattga
    26221 agagcaaatt atcttcaata aaagagcaat acagttggaa aagaaaaagc gatccgaacg
    26281 acaggccaaa acctatcgtt cggatcacta tttttctaga actgcattca agagactatg
    26341 acaaaaagca tgccaaggcc ttttttcaaa taacagtgtt ccatcattca ttactttgaa
    26401 ttgcttttag cacacttctt gaaagcgggc caacgatgat caactgcaac ggtaaagcgg
    26461 caatcacatt cattttccaa gcattcatat aagcagagaa taattgatca ggtattcctt
    26521 gttccatgat cacaccgaat aaagacatac atgtcaccat tcctaaaacc atcaagcatg
    26581 aaatagttaa gatcatttgg accttgcttt ctggatttac tggtagttta aaggcaattt
    26641 ttttcgccag cacaccgatg atgaagacat ccaaaatgaa agccaccacg aatccaggca
    26701 ctagaccaat caacaaactt gataaatgaa gatcttgatg cagccataaa ttatacgcgc
    26761 tcatccccaa gaccatcaaa aaacacatca ttgttgtaaa aattattcct tcttttttat
    26821 ttgtaggcaa tctgtaccac tcctttaaat ttgtcacgaa aatcatcgta gcacatttaa
    26881 aaatttctcg taagtacttt ttttattttc tgccctttta tttcataaac ttcatcagcc
    26941 gtacgctcga caaagtaaga atcatgagaa gtaaacaaaa tcgttcccgg atatatttgc
    27001 attagcttct ctaaagcttc gatcgttgcc atatcaataa aattagtcgg ttcatctaac
    27061 agcaacacat tacttggctt agtaaaaagc aaagcaatcg tcagacgggt cgcttctccc
    27121 ccacttaatg tacataacgg tttcgtcaaa gtttcctgag caaaccctaa gtgattcagc
    27181 aaactacgga caagcgattc tgaaaactct gtttgtctca tgacaaaacg aatgatggat
    27241 tcttcagatg tcatttgata acccttttgc tgatatactt gaaagcttac tttcggagag
    27301 agaaggattc cttctccttg tttgcggata tgttctaata aggttgtctt acctgaacca
    27361 ttttcaccga caatcgcgat ttttttacct aaagaaaact gaaaatcacc atttaccagt
    27421 aatgttctcc cacttctttc caattggaca ttttgtccca tgatgggata gcggctgtgg
    27481 agttcaagag attttggtac tggaaaacgg atttgtttca tctgctgcgg cttggttgtt
    27541 tctcctattt tttggagtct tctctctaat gttttcgcat tcttttggat ggctttttgt
    27601 acactgtctt tttgtttaga ggaagacaac cgatccggtc tgatttgttg ctttttcttt
    27661 ttactggaca ctttttccaa cttttccgct tgttttttct tttcttggat cgatttttcc
    27721 aatcgttttt tctctttcat gatcgactct gcttctcgta gctgagtctt tttctccaat
    27781 tccttttgag tgagataggc actataattc cccgaaaact ctcggatttc tccgtctttt
    27841 acttcccaga ttttcgatgc tagctcatct agaaaatagc gatcatgact aacaaaaacc
    27901 agcgtaccat aataataacg aagctcttca atcagtcgat caatactttt tctatctaga
    27961 tgggttgttg gctcgtctag aagcatccct tgttcataag aagaaagaag acgtatcaac
    28021 tcgatctttc gttcttcccc tccgctccat ttccgctctg aagtttcttc cagtcttaac
    28081 tggaaatgac tggctaattc caaatttagg tcctttgctt cagcgagata ggttaattga
    28141 ggcaggtagt tgaaggtgat ctctgtttgg attttcccat gatcgggaaa aagttcaccg
    28201 gctattagtt tgagcaaagt cgatttaccg acaccatttt ctcctatgat ccctatacga
    28261 tcttgctgat agacacgaag agaatcaata gatagttcta gctggttgcc aaagctgact
    28321 tgtagatttg ttatatttac tgctaaattt tccatatgtt ttacactcct atttcatgta
    28381 aaacaacaat tatcgatact aaaaaagagc agaaaaatct gtttgcaaaa aaactgcatc
    28441 acatcttttc ctactctgaa cgaaataact atgactaaat caccaaatct gtcatagctt
    28501 tcacctattc tactgtcgtt cgaaaaaatg gacatactcc atccatgaaa ttgttgttta
    28561 tacatgtctt ttgaatttat ttagttgtta ttcaaaagtt cgaaacgtaa tttcatcgat
    28621 gcagtcattt ttccgacctc cttttttatt aggttaccac acacgattca aaaaaagcaa
    28681 tagctactga atagtaactg aaaacctttt attcgaacgt agtgatcttt tctggtgcta
    28741 aacgaacaga ctcgtaacct tcttctaccg cttttcctat tgataaaata ataactggaa
    28801 cataacggtc tttatccaaa ccaaatgcct ctgccaactg atctgcttcg aatccaccaa
    28861 ttggatttgt ttcatagcca tgatcacgcg caactaacat gaattgcata gccgctaagc
    28921 tagaatcgac tttgactaca tcgttcattt cttctcttga aaaactcttg taataaggaa
    28981 tgatcgcagc taattgttga tctctgactt cttttggcat cttccctgat tcgtaagctt
    29041 ggttatagat ttcttcgcca tattcgtaac attgcatatc gccaaaaatc atgaccattg
    29101 cagaagaagt atcattttgt ctcgtattga aacggatcaa aggacgcaag atttcttttg
    29161 cttcctcgct ttcagcgacc acaaagcgcc atggttgcat attgacagaa gaaggtgcag
    29221 tcgtcgcttc tttgatcatt tccagcattt cttcgtgtgg gattttgtat gtttcatcat
    29281 atacgcgaac ggatcttcta ccagtcataa ttgttgaaaa atcatttttt tgttccatta
    29341 ttattaacct ctttcgtttc tgttgttata ttgcaaagtt gggtattcaa tttatctaaa
    29401 agtgttagaa attgtttcat ttctacatcg ctgaaagccg gggatagcac ggaacctgta
    29461 tctttatggt ccatttcaca atgctcgatt ttcttctggg ctttttctgt tagcgctaca
    29521 tacacttccc ggttgttctg aggatttctc tgacgcgaaa catagccatt tgcttccaat
    29581 accttcagat gacgagtaac tgctccttga tcaatctgca acttttcttg caaactgctt
    29641 tgtagcaatg gattttcttc tttcagtctc atcatgatct cgtatctagt aagacttata
    29701 ccgatatttt cttcaaagat ctggacgatt ttttgatcgg ctaatttgat ttgatataaa
    29761 aggtgactaa tctcttctaa agacataatt tcctcctttc taataattga ctcgtcaacg
    29821 attgacttgt caataataga ataaaaaaag aaaaaagtca aagaatcagc tgtgtaataa
    29881 aaaaaacgct ctcaatagaa aagtatagag aaaagaaatt ttttgtagaa tgaacggaaa
    29941 aaatgataaa gaaagcacaa cgctaaaacc aagattattg attcttatca aactatagaa
    30001 gatagctctt ctgcagtaga aaattttaca ccgccaaaaa tttccgaaat cgccttttgc
    30061 aagaatcccg tcgaatatta gaaattggat cagtatgctt aaacagtaat aaaaaagtta
    30121 tgacaagatg ttgtcacaac cttttttgag taaacggtgt tcaaaaactt gcatctgcgg
    30181 taataagttc aaccaaacaa aaaacatgaa aatctgtttt tgtttggctg ttcttactac
    30241 ttgatgcagg gcagcttttt cactacctct ttttaataac cataaaatcc tattccttca
    30301 tatctccagc ccatcggtgt tagccaatct ctttccttag cactcaaggt atagtgatgg
    30361 gtaccagttt tttcatttgg attaaatagt cgataaattg gcttggaacc accagaataa
    30421 aaacttagac cttcataacg ccaaccgatt tttgttaaag agttgatctc agattgtgcc
    30481 attgtgtaat gatggtctcc actgtaagga ttgtataaac ggtagacagg ttgtccgctg
    30541 tttggtgctt gccaagcaat cccttcatat tcccagccta cagttggcag ataatttttt
    30601 tctccggcat tcaatgtata taaatgttct cctgtatgac ggttatacag acgatacatc
    30661 ggagaagttg aagttggtgc tgtatcatac tgtgtcagat tataggtttc gattacattg
    30721 ttcaatgtag aaccataatt aggcgcagtg gcataacgac ctgttagcca agccgtagca
    30781 tctttgtaag agttcgtatt gcttttccat gcaccggaat agtagtaaac gcctggctga
    30841 aaagaagtcg tcttcaatac cgttacgtta tcttgcaatg attctttgta cgaaggatat
    30901 tttctaaaag cggcattcac cgtaatccat tgcccattca catattccga agtaggcatc
    30961 gtaacagatt ggccattata gcttccttta atcccaaata gattgtaatt aggggcagaa
    31021 gccaaagtac tgttccccca gccactttca acgatggctt gagcaatcat gactgatgca
    31081 tataagtcat tggcacttgc caaactttgt gcagttgagg caatactatc aatgaacgct
    31141 tgttgagaat tagcagatga gcgagcataa aaagcatagc cagtatagtt ttcgaattca
    31201 gacaaatctg cttgatgggt atgtgtcaat gtttgcccat ctgtcaattg gttcgtgatt
    31261 tcttctgcat tctctggatt gttctgagct tctgctgatg tactgtcttc ggtttgtgtt
    31321 tgttctgttg tttcagttgt tggttcttcg gttgaaattg tattttctga gacagtagct
    31381 gactgcaaag tactctctgt atcgttttca attcctgttt ctgaagaact cgatgtctga
    31441 gttgcgacag tttctgtcga ttcttcagct agcactgttg tgttggcaac tgctagattt
    31501 cctaaaagaa taacgctaaa taataaattc tttgctttca tcttttttct cctttgaatt
    31561 atgaaacttt tcctaaataa cagtgtaact aaaaaagtaa aagcaagtca atgtccatat
    31621 ttaaataaca tttgtttctt tttgataaat ttgctttttc tacataaaaa gacgtaaatg
    31681 aagaatattc ttctccattc acgccttaaa ttgatggtcg tttgaataac tatttaacaa
    31741 gtaattctct ttggatatta tccgttatgg attatacttc ctgtgtcgtc ggacggataa
    31801 tcatgttact gacagacatc cggtctggcg tttctatcgc aaatgctaca gcagaagcta
    31861 tatcttcgga ggtcaatccc cattcttttt gcgcttcatg aagttcaaga gcagcctttt
    31921 tgtcgctgat ggtttgatat aattcagttt gaacagctcc tggagagatg atcgtagatt
    31981 tgatgttatt ttgccgttgc tcttgacgta atccttccat gattgcacgg acagcaaatt
    32041 ttgttccgca ataaacggcg gaatcaggat aaacaacatg tcccgccaca gaatcagttg
    32101 aaatgatatg cccagatttt tgtttttcca tgattggaag tacggcggaa attccattta
    32161 acacacccat gatgttgata tcaagcatat tttgccattc atcacggtga ccttcaacta
    32221 atggagcggt tggcatgatt cctgcattat tgtacaatac atcgattcga ccgaactttt
    32281 ccatcgctaa atcgataact gcttggactt gtgcaaaatc tcttacatct gctgtttgaa
    32341 tatatagctc agcttctggt aaagattctt ttattgcttt taggcgatct tctcggcgtg
    32401 cagctataac tagtttagcc cctttttctg ctaatagctt cgttgttgct tcaccgattc
    32461 cgcttgaagc gcccatgatg acaatcactt tttctgataa atcattcatt ctattctcct
    32521 cctgttttga aaaatctctt gcttatactc actatctctt atttcatctt actgttcaaa
    32581 taatccgttc gacaggtttg acgattttgg cagggattcc tgcaacgatg gtattgtctg
    32641 ggacatcttt cgtaacagtt gcattcgctg caacaatggc attctcgcca atcgttacgc
    32701 ctggcagaat agttgctcct gctccgatcc aagcattttt cttgatgtgg attggcttta
    32761 ctgacaagcc tcttcgttct tttggatcgg ttagatggtt caccgtgatg aggttgacac
    32821 gaggaccaat caatactgaa tcttctaaac agatgccacc taagtcaacg aacatcactt
    32881 gacgattgat aaagacattt ttccctatac taatatgttt gccgaaatct gtataaaaag
    32941 gcagcagaac agtcaccgta tcatctactt tgtcactaat gatattttct agccgtttgc
    33001 ggatatctgc tggttcatgg tacccagtat tcagcgctgc taaaggctct tcgttttcag
    33061 cttggatctg atggatcact tcaaagagcg gatccgttac tcgaatctct ttattgacga
    33121 tttgtttttc gatgaggctt gtcattttgc tccctccttt tatccttact ttataacgga
    33181 cggcttatca tgtctaatac ttataaaaca tacttaacta ttccttttaa ctatacttag
    33241 tggtatgctt ttttaaaaac aggagggaaa aaatggatat tcgtatactg acgtattttt
    33301 tagcagtagc tagagaaaaa aatatcagcc gagcagcaaa atctctgcat atcacgcaac
    33361 ctactctctc aaaacaactg aaagatttag aagaagaact tggtgtgaca cttttcactc
    33421 ggggctcccg agagatacag ttgacggatt ctggtcgata tctttattca aaaggaaaag
    33481 aaatcttgtc gttggtagaa aaaacggcga acaatttaac aaatgacgca atcatctctg
    33541 gagaaatcta cattggcgga ggcgaaacta aagcaatctc tttcatttct gatagccttc
    33601 attcgttgat caaggcgcat cctgatatcc agtttcattt atatagcggg aatgcagatg
    33661 atatcataga caaactggat agcggattac ttgattttgg actcgtaatc gaaccgacaa
    33721 acaaacaaaa ataccagtca tttcgactgc cagcccaaga tcgttgggga cttttgatac
    33781 atcgaaatca tcctttagca aagcaaaccg tcatccatcc gcaggaactg aaaactgttc
    33841 ctttgatcat ttcccggcaa acgtcagtgg acagtcaact agctgaatgg ctaggtttca
    33901 gcatagatca gttatcgatc gtcggaacat ataacttatt gtacaatgct tctttgatgg
    33961 tcgataacgg gacttttggt gcattagctc tagatgggat catcaatact tatgggacgg
    34021 aaaaaatatt catcccactc gatccgccat tacaagcttc actgaatctc atttggaaaa
    34081 aagaccagcc tctttctcct gctgctcgta aatttttaga tgtcctaacg gagaaataca
    34141 gttaagtaga gtttttttcc ttcgctttca gctttttaac aacgaataag ataagcaaaa
    34201 gatccaacga ttaaataaca taagtttctt aatttatttc ttccttttat ttctgataat
    34261 tccagtaaat tactataatt ataaaaaaag aattgacaaa acataactgt ctatataaaa
    34321 ttgattacgt taacaataat tcgtttcatg tgactagatc atactaggca tgaaaaatct
    34381 ctgaggaaca tggtttccct aggaggtttt tcgtgtctat ttttgtttgg ccgcaatcaa
    34441 ctggtctagc aaacgtgaaa accaaatctt caaaaagggt gcgcgcatga aaatcaaaaa
    34501 gatctttaat caaaatgcag tattggtaga tgacggtgga caggaaaaag tagctattgg
    34561 aaaagggatt ggatttgaca gaaaaagaaa tgatttgatt tttgatcgtg atgtcgaacg
    34621 gatatttgtc atggaaccag aaggtcaaat caagttgcaa aatttactta atcagattga
    34681 tgaacgcttt ttatttgcag cagaaacgat cattgatcat gcggaaactg ttttgatgga
    34741 aaagttgaat gaacatgtat tgatcgcttt gaccgaccac attgcttttt ctgcggagaa
    34801 cattaataac ggtatcgtca tccgcaacaa acttttaaga gaaatcgaag tgctttacag
    34861 tgatgaattc agtattgctc aatgggcagt agagtatctg acaaaagagc ttgggattcc
    34921 atatacttat gacgaagcag gatatatcgc aatccatatt cacagcggac gaagcggtcg
    34981 gaacaataac catcgcagta tccgtgaagt gacgattgta tctgacatca tccatttagt
    35041 ggaatcagaa ttggaaacag atatccattc agaaccattt tcactgaatt attcaagact
    35101 ggtgaatcat ttacgactgc ttctacaacg aacccatgcg caacaatatg ctgttttaga
    35161 cacggaaatc gtagaaatgg tcaaacgaaa atacccagaa agctataaga tttctaaaaa
    35221 aatccgtgtg ttgctgacca aagaatacca gctcgcgatc acgaaagaag aactgggtta
    35281 cttggccatc catatcgagc gtttaagaag cgcaatcgta caaacaaatg ttaacaatca
    35341 tgaggaggaa aaaaattgaa agcatatcta caacgaatgg gacgttccct tcaacttcca
    35401 gttgcggtct tacctgctgc tgctttacta gtagggatcg gaaactggtg ggcatcttat
    35461 agtaacgaca ttgtcgctca ttttttacaa gctggcggta acgcagtttt aggtcaatta
    35521 cctttgctgt ttgccgttgg gttagcacta gggatgtcag tggacaaatc aggtgctgct
    35581 gccttggcag gtttggtcgc atttgaagtt ccagtaaatg ttttaaaaac tgattcagta
    35641 gctactttat tgaatatcaa accagaatca gttgatccat cttttgctca gatcagtaac
    35701 gtatttattg gtattattgc cggattgatt gcggcagctc tttataatcg tttccacgaa
    35761 gcaaaattac cgatggcttt atctttcttt agcggcaagc gtctagtgcc aatcttggct
    35821 gcctttgtca tgttgctgat cagtgcagtg ctactgctcg tatggcctcc ggtttactct
    35881 gctttagttt cttttgctaa attcattttg agtttaggct ggggtggtgc aggtctttat
    35941 ggcttcttca accgtttact gatccctaca ggattgcatc aagcaatgaa ctccgtgttc
    36001 tggtttgacg tagcaggaat caatgatatt ggtaaatttt tggcttctga aggaacaaaa
    36061 ggcgtaacag gaatgtatca agctggattt ttcccaatca tgatgttcgg gcttccagca
    36121 ggagcatatg ctatttacag aaatgcacgt ccagaaaaca aagcaaaaac agcttccttg
    36181 atgctagcag cagcttttgc ttcattcttt actggtgtga cagaaccact tgaattttca
    36241 tttatgttcg ttgcttggcc attatatgtc ttgcatgcaa ttttcactgg gatttcttta
    36301 gcagtgagtg cattcttcca ttggacagca ggatttgcct ttagtgccgg atttgtcgat
    36361 tacttcctat ctttgaaaaa tccgattgcg aatcagccat tgatgttgat cgtccaagga
    36421 ttagtaacag cagtgattta ctactttggc tttaactttg ctatcaaaaa attccatctg
    36481 atgactcctg gccgcgagga agcagatctt tcagatgacg atactgcaac gactaattca
    36541 gacaataaat atgcggcaca agcaagcaaa atctacgctg cattaggcgg agccgacaat
    36601 gtaacttcga tcgataactg tactacacgc cttcgtttgc aagtcaaaga tacaggcctg
    36661 atcgatcaaa ataaaatcaa agccactggc gtacctggta tgaagatcat cgacggaaaa
    36721 aatgcgcaag tcatcgtagg tacagaagta caattcgttg cagatgagat ggcaaaacta
    36781 catggtggtg ctgcagcacg acctgcacaa acaaacaccg tggttaaaac agaaacattt
    36841 gccggtgaaa cgatcgaaca agatatttac gctatcgcaa atgggaaact gattccaatc
    36901 actgaagttt cagatgatgt attcgcggaa aaaatgatgg gtgacggcta tgctgtatta
    36961 ccagaaaatg gagaaatctt ctctccaatc gctggtacga tcacaaacat tttcccaacg
    37021 aaacatgctg tcggaatcca aacagatgct ggtatcgaag tcttgcttca tatgggcatc
    37081 aacactgttg acttaaaagg cgaaccattc actttatatg tagaagaagg tcaaaaagtc
    37141 gcaagaggtc aattgattgc tttagttgat cttgccgcta ttcaatctgc aggcaaaaac
    37201 acggatatgg tcgttgtatt cacaaatggc gacaaagtcc aaagcttaga aatcgaaccc
    37261 gcacgtgatg taaaagcaaa tgataaaatc ggctctgttt ccaacaaagc ctgagccatc
    37321 gtcttcccca gactttttta tcgataaaca aacaggagct gagacaaaaa tcttgtctca
    37381 gctccttttt ccgaataaaa acggcgttct cctgccaact ccgcggtaat aagtccagct
    37441 aaacgaaaca tatgaagagc tattttttat ttgactcttc ttattacttg atgcagaaca
    37501 gctttttcac aaaatttttc ttcatactca aaactaatcg gctttttcac aacctcttta
    37561 tacattattc ctgatccaat atttctttta atgctgcttc aagagaagtt aatggttttc
    37621 ccaatacttt ttctaaatca tcagaggaaa tagctaactg atccttttta atatcatgtt
    37681 ggatcgctaa aaacatgtca gctgcttctt gagggaatcc ggcttctttt agattttcga
    37741 caaacgctgc atcacttgct tcaataattt ctaactcttt acctgtcgca cgctctaatg
    37801 ccttggctaa ttcttcatag tcagttagat tgccggataa ttctaagatt tctggagagt
    37861 cactttctac tagtactttt gctgcagctt ctgcatattc acgtttcaat gcccaacctg
    37921 tttttccatt tcctgcagca taaacgaatt gaccagtttt taaagcctgc ccaatgatag
    37981 gtaattcatt ctctacatac caattattcc gtaaaaatgt atgggaaata cctgaatcct
    38041 taatcatttt ttcagtgaat tggtgatctg gcgctaatac actgatcgag tgatcggcat
    38101 ctgcaaaact tgtataagcg atataagata ccccagctgc ttgtgctgcc ttcactacat
    38161 tcttatgttc ggcttgacga tttcctggaa cgccagagac aaaaagcaat cgatctattc
    38221 ctttcaacgc ttctttcatt gagtctaaat catcataatc accaatacgt atattaaatc
    38281 cagcttcttt cagtgcttct cctttttctt cactacgtac tagtacatag atttcatttt
    38341 gtggaaccat cttttttaag aattccagcg cataaccacc aaaacctcca gttgctcctg
    38401 ttactaaata atgcatattt gccactccct attcttttta tttgttaaga tttacagctg
    38461 tacatttata aattacacct ttatcataaa gagtgtggta agataagtca agcaaaaaaa
    38521 atgaaaggat gacaactgat gaagtattct ctgcaattta gtgatgcaat ccatgtactt
    38581 gcctatattg aaatgtttca agatacccga tcactttcta gtgagatgat tgccagtagt
    38641 atcgaaacaa atgcagcaaa tgtacgtaaa atcatgagtc agctcaaaaa atccggtttg
    38701 atctttacaa aaaccggaaa accgcagcct tcccttgcca aaagtccaga tcagatttct
    38761 ttactagatg tctatcgcag catcgaaggt aatacacagc ttatccaagt cgatccaaaa
    38821 acaaatccaa attgcgtcgt tggtgcgaat atccaagaag ctttagaaga aagctacgct
    38881 cggctgcaga aaaaggcaga agaagaaatg gctaagatca cacttgatag tctgatacat
    38941 aaaatagccg ttttggaaaa agaaaaaaga ccagaaaaca caggcctcgt agagagattt
    39001 ttataattct ctctactgtg cctgcatttt tctgatcgga atcgtctaca taaattcttt
    39061 tattctccgg gggacaaaag ccttgtcatg cccctttttc ctctctaatg ctcaggtcaa
    39121 agcgctgaag tcatgacgtc ttgataaacg gtgttcaagt agctgaccct cggcattagt
    39181 tctgaaaaat gcaaaatatg gaaagctatt tcccattttt cctctctaat gctcaggtca
    39241 aagcgctact ttcacgacct ctatattatc atggtttatt gactaccggt atccagattt
    39301 ctcccatata tttcccgttg gtaactccca tttcgactac agcatttgga cccccaacat
    39361 aggcgtaatc ttctacttct cttaaaacat gcccaaaggc cgtattcgtc aaatcatttc
    39421 ttaattcttc aggcgattct gctaaagcgc ttacaacaag atacgttcct tttgggaatg
    39481 ggatgattcg cgtagtgttt ggaacatcct gactcgtttg aacaccgaca tagtacatca
    39541 ttttcccgtt atagggttcg ttgactgtaa agaaataatg attttcagca gcttgtttca
    39601 atgcatctat tgttccatta ccgataactt tttgctcaaa tgcttgtttc tctgcagcca
    39661 aaccgacaaa atccgtattg ctactcttca attccacacc aatacccgtc accgtaaaat
    39721 ctggtttttc ttcgatccta tactttgcca tttctcccac cctttctatt atattaagtt
    39781 catcttacat aaaattaaag ggaaatgaca cgattacgct cttttttcta gggatatttt
    39841 tattttttaa ctattttttt gctttcactg caatccaaat ctcactgtaa acattactga
    39901 aatcctctcc ccattttgta aaagagatct cgggagcttc gactaaccca taatctgaag
    39961 aaggaagcca ttctgcatag attttccccc aagtatcttg taaagtttgg ggaaatggcc
    40021 ctttattagg aaaaatggcc caagtgtgtt cttctatagc aagcatttct aaatcagcgt
    40081 acggattttc ttttgtcgta gcaaagccaa tcatgtgcat caaactgccc ttttcttcca
    40141 agcggtctcc atcaaatcca taagaaacgt tgattacttg atttggatat aaatctccta
    40201 acttatgcat ctcttccctt tgagtctccg tgatcgtctg cgctaacttt tgtatttctt
    40261 gattgactcc ttcaaatttg acaggaactt ttttcgccac acctgccaca taaaactgtt
    40321 ctttcttttc gatacgaaat tccatcgatt ttcctcctct gacatcaata aaaaatgtca
    40381 agcgtggaaa agatttttgt atgccgtatc tactgatttc tgaaggcaga tatcctgacc
    40441 aatcacgaaa agctcttgaa aaaccttcga ctgtttcata tccatatcta aacgccgttt
    40501 ctgtcactct tgatccatct agaagatcgc gattagccaa agccaatcta cggtttttga
    40561 tatagtccat tagcgacatc cctgcaataa acgtaaagat gcgttttaat tgatattcag
    40621 agattcctgt attttcggct attattttat aggaaagtcc ttctgtcaaa tgttcttcga
    40681 tgtaatcgat caattggttc agatctttaa gcatatttat cttcctcctt gattgccttt
    40741 ttatcatatt atcaaagaaa gtcaattact tgatgaatca ggaaccaatt tagtcagatg
    40801 tagaaaatgt tgtatggatc taaagaaaat tctcaagcag cgtttcgtaa cggataattg
    40861 gtattatctg cctatctatg ttttaccttt agaaaaataa attttgggga agacagattc
    40921 taagtaaaca aagcggtaaa aatatctttt attcccttag ttaatccgct tactacctac
    40981 tgtacaaagc aaattgttgt tcaaactgct gatcagaacg ctgcttggat ttttctttgc
    41041 gatacatcat acctctttta tcaattggaa caattattta tttctaacaa aatcacttca
    41101 tttttgtcag ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta
    41161 ctttttctca cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa
    41221 agtgattatc aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat
    41281 ataaataagt gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga
    41341 aaacgttttg gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta
    41401 tacaagcaag tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac
    41461 ttgggatgaa aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg
    41521 caaacacatg gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta
    41581 ctcactttcc gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct
    41641 tttaacagag attcttatag aacatccgca ctttatcgaa cgcttcaaag ttaagatgcc
    41701 atttcatgca ataaattatt cagataaatt aacaactatc atccataaat acgatcatat
    41761 tatcaagaaa aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat
    41821 ccaagaagca gttgaatcgc ttattcagca aaatgcctat cttcccgcat tcgaattgat
    41881 caatgaagtg attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct
    41941 tcttctgata gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc
    42001 agaaaaaagg caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta
    42061 ttcaaaacgc tactctataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa
    42121 ccaaaagaaa aaaatagata aaaaactaaa gaaacggtaa agaaagacga tttcaatgaa
    42181 aaatagcaag taaacgataa caaccatcct aaatccacaa gaaatacagc agaccttgct
    42241 ttaaaaacac gaatcatgca aaggatgaag taaagaagac ttaacgcgta cccaatatta
    42301 gtcatttgac tattaggtac gcgttttgtt ttataagtca tgacaaattt ccttcgttgc
    42361 tggatagatt ttccggcatt gttgatagat tttatgatac ttttctacat tttgtaagag
    42421 aggttccgct gcatctgtat attggataaa attctgacaa caactttcag gtgtgtcgaa
    42481 ccaccctaac cctagtgcag caatcattgt tgcgcctaat gcaggtcctt cctccatact
    42541 taaacaatag accgttgcat cgaaaatatc agcttggatt tgtagccatg caagattttt
    42601 cgcgcctcct cccaccgaaa caatacgtgt aaatctgcga cccgctagtt ctttacgttt
    42661 caatcccacc attgacaaat gctcattaaa aatttagaat attcacaagt ttaacgacaa
    42721 ttatgaaaat ttatgtcgtg ctaaaggagt tttgttattg gatgaaaagt tagatataaa
    42781 aactgcgatc aaagacacgt tacctaccgt tttcggttat atcggtattg gacttgcatt
    42841 tggtatcgtt gggaaagctg ccggatttca tccactagtc gtcacgttga tgtccctgct
    42901 ggtctatgct ggttctgccc aatttatcac agtcagcatg cttgctagtc acagcccatt
    42961 gctttccatc gttttctcga cctttctagt caattcccga atgattctga tgagtatgac
    43021 gattgctcct tatttcaaga aaaatcggct gcttcaaaat ctgctgattg gcacattgct
    43081 gacagatgaa agtttcgcac tgggaatgaa taaattgaac tatactggac aaaagttaaa
    43141 ctttcgttgg atgaatacag ctaattggat ttcttatttg acgtgggtgg gttcttctct
    43201 tgttggcgca cttttaggga attttattac tgatccgaaa aagtttggtt tggatttcgc
    43261 catcgtcgct atgtttatcg ggttacttta tcttcaagtc atttctgata gaaacacctc
    43321 taaacgatta cagttgatcc tgattggtct cactttgatt ctggtatatg tcggactgat
    43381 ttttatccca agcaacttag tgattgtagt tgtaacactg attgggtgcg gattgggggt
    43441 gtggatcaaa catgccttct tttgagttta cattgctgac tattttaggt tgtactttag
    43501 caacttggct ttctagggtc ctaccatttg tcttactgaa aaaattcgat ttaccgcaac
    43561 cgttgcttga gtatttaagt tttgtcccga tcgtcattat gtctgcttta tggttcagca
    43621 gtctatttac ccaaaatatc ggacatctgc cgcagatcaa tctcgaaaat gctttggctt
    43681 cagttcctac acttttagct gctattcttt caaaaagctt attggtgatc gtcttggcag
    43741 gaattctttc cctttcgttg attcgcttat tataaataaa aaaacaagag cttatggcaa
    43801 attactgtca tgcgctcttg tttttatcaa agtcaaataa gaaaaaaatc ggcgtattct
    43861 tgcttatccg ttgaacggac cactaacaac gacttttcct aacatatgtc ctgtttctac
    43921 gtctttggtt gcttgtttca agttcttcgc attcatccca tctgaataga ccttgctcaa
    43981 tgtactgcgg ataatccctt catttgctaa agcagcaatg tgcgctaaag cttctccttg
    44041 cgtttcgatt tcatagttat agtctgtttt agcgaacata tactcccagt cgaagctgat
    44101 agattggttt ttccaaacac ctatatctaa cggctctttg atccctacga tcgttcccac
    44161 atgtccaaat ggtcggacaa ggtgcttgat ttgatcaaag tagcgggtaa tatcgaataa
    44221 tacagcgaca tactctactt gatcgattcc taattcacga aaacttttac ttaagtcgtg
    44281 atgataatcg attggatagt ctacacctgt ttttttcagc cattcaaaat tcttcggact
    44341 tgcagtcgcg tagacttcta ggccagccca atgcgctagt tgattcaaaa tcgaaccgac
    44401 gcccccggat ccgttgatca ctaagatttt tttcccgcga ttggcatttt cttctggagt
    44461 tagaccaaat ttttcaaata acaattcata agcggtcaat gaggtcaacg gcaaggctgc
    44521 agcttcttca tctgaaagat tttttggtgc aagagcgaca atacgttcgt caactagttg
    44581 gtattcttga ttgcttcctg ctcttgttgt tgtacctgcg taaaaaacgc gatcgccgac
    44641 agaaaatttt tgcacttcgt cacctacagc aacaactttc ccgactccat caaaacctaa
    44701 gatacgtaaa gcatttctta tcccgttatc ttggcgaagt ttcgtatcga caggattaac
    44761 agatactgcg ttgactttta ccagaagatc ccgtgaactg ggtacaggta tctgttcttt
    44821 ttcatcaata aagctttttg gatcatcgat cggcaaccct tcataaaaac cgactacctt
    44881 cattaattcc ttttctgaca tacgaattcc tccttagctt catcaagttg gagcatccat
    44941 acagtgttca tttcaatata tatagtatgc tatttgttcc acctttttta ttcatcataa
    45001 ttattctctt acttaaagta aactatttgc acttttttgg ttaaaaaaga ggttgcgaca
    45061 agacttttgt cacaacctct tataaacggt gttctcctac caactcttcg gtcacaagtc
    45121 aaaatattca tcaaacatga gaagctgttt tttgaaattg ctccttgtga ctcagagtta
    45181 aacggcaggc tcacaacctc tgatataaac ggtgttcaat caactgaccc tcggtattag
    45241 ctcaaaaaac gacaaaatat gaagagctat ttttgtttgg ctgttcttat tacttgatac
    45301 aggacagctt tttcacgacc tctaattcac ggtgttcaag caagaaatca ttgttcactg
    45361 aaaagcgttg gatgttcttt ttcaaaggtc tctaagatct ctcgtacttc atcagtagat
    45421 ttcgtattca tcaactgaac tcgcaagtca tttgctcctg gaaatccttt gatatagatt
    45481 ttaaagaaac gatgtagtcc tgtgatggaa cgcgggatga tttctgaata atgatcttgc
    45541 agatctagtt gcagacgaag tagtccgatc agttcttttt ctgtatgttc cctcgggtct
    45601 ttttcaaatg cataaggatt cttgaaaatc cctcggccaa tcatgatgcc atcaacgcca
    45661 tatttctcgg ccaattcttc tcctgtctgg cgatcgggga tatctccatt gatcgtgatt
    45721 agtgtttgtg gagcgatttg gtcacgtaaa gcaatgattt taggaatgac ttcccaatgt
    45781 gcatctactt tactcatctc tttacgcgta cgcaaatgga cagaaagatt ggctatatct
    45841 tgatgaagta ggtgactgat ccattcttcc atctcagaca tttccgtata gccgatacgc
    45901 gtcttgacgc tgacaggcaa accgccagct ttagctgctt caatcaattg tgccgctact
    45961 tctggacgaa ggatcaagcc actgcctttt ccacgttctg ctacattagg gactgggcag
    46021 cccatgttga tatcgattcc ttggaagccc atttctgcca tagcaatgct catttctcta
    46081 aaaaattctg gcttgtctcc ccaaatatgg gcaaccatcg gctgttcatc ttctgtaaat
    46141 gttaatcttc ctcgtacact gtcttttcca tctggatgac agaaactgtc tgagtttgta
    46201 aactcagtaa agaagacatc cggtgctgct gcatgcttta cgacatggcg aaatacaaca
    46261 tctgttacat cttccattgg tgctaaaaca aaaaaaggct ttggcagctc tttccaaaaa
    46321 ttagtagtca tttatttgtt actctccatt ctatcaagca gtgaagtcgc aatctttctc
    46381 aaaaacattg tcattatact aggaaacaaa aaaagaagca aagtgaacag cttaaaagat
    46441 gtttctgatt gagcaaaaaa aagactgtga caaaaatctt atcacagtcc attttccgga
    46501 ataaatggtg ttcaaaagct tgcatttgcg gtaataagtc caaccaaacg aaaaatagga
    46561 ggagctattt ttgtttggct gttcttatta cttgatgcag aacagctttt tcacaacctc
    46621 tccactaaac ggtgttcaat cagctgaccc tcggtattcg ctcaaaaaac aacaaaatat
    46681 gaggaactat tttcccgttt gtttctctaa tactcaggtc aaaacgctga tttcacaacc
    46741 tcttttctaa cttctcaatc ctcttccttt attgatcaaa taccaagtaa cggcgtacaa
    46801 aacaacgatg aataacacaa gtacgatcat cgatgttgct actggcacat cgatcgttcc
    46861 aaggaatccg taacggaaac cggagatcat ataaacgatc ggattgactt ttgaaacagc
    46921 ttgccaaaag ggcggtaaca tagagatggc atagaaaacg ccacctagat aagttaacgg
    46981 ctgcaagaca aatgtaggca cgatcgatac atcatcataa gactgagcaa aaatcccgtt
    47041 gagcagtcct gctaatgaaa acaagattgc cgtcatcaat aatgtaatga tgaccatgaa
    47101 ccatgaatac acatgaagtg gtacaaagaa caaggaaatg atcgttacga gagagccgac
    47161 gagaatactg cgacccacac caccaatcac aaagccccaa ataatcacat gcgtaggaac
    47221 aggtgctacc aagatttctt cgatattttt ctgaaatttt tgagagaaga aggacgaaga
    47281 aacattggca tacgaactag tgatagcaga catcataatc aaaccaggaa cgatgaattc
    47341 catatatgaa aaaccaccca tgtcaccgat ccgcccaccg atcatttttc cgaaaataac
    47401 aaaatacaag gatgtcgtga tcactggcgg aaccagtgtt tggacccata tacgcaaata
    47461 gcggttcgtt tccttgatag ctaaactttt taatgcagta aaataaagat taaacatttt
    47521 tctcctccgt atgattattt tcttctgtga ttttcaagaa cagttcttct aaacgattcg
    47581 acttattcct catagaaaga actttgattc cttgtcttgt aagctgatcg aagattccgt
    47641 tcaccccttg atttcgttca acttcgacag cgattgtctg ctcatcttca aatgtacaag
    47701 catatccttc aatcacaggt ttggtatcat atggcgctaa atcaaagata aatgtttcaa
    47761 attgcagttt tgacagaagc tctttcatac ttgtattctc gatcagttca cccgattgga
    47821 tgattccgat gttgcgacaa agcatttcag cttcttctag ataatgcgtc gtcaaaatga
    47881 tcgttgtacc attttcgttc aattctcgta gaaactccca catctcgcgt ctcaactcga
    47941 tgtcaactcc tgctgttggt tcatccaaga tcagcaaacg tggttcatgc atcaatgccc
    48001 tagcaatcat cagtcttcgc ttcattcccc cggaaagcat acgtgcacgg acatttctct
    48061 tttcccaaag attggactgc ttcaaatatt tttcgctgcg tttcatcgct tctttgcgag
    48121 agacaccgta gtagcctgct tgattcacga cgatttgctg gaccgtttca aaagggttaa
    48181 aattgaactc ttgtggaacc aatccaatct gttgctttgc tcgaactaga tccttgtcca
    48241 gatcataacc gaagacattc acttttccag atgttttatt gactaaagaa gtaataatcc
    48301 cgatcgttgt agacttccca gctccatttg gccctaatag cgcatagaaa tctccttctt
    48361 tcaccgaaag atcgattccc cgcaatgctt cgaccccact tccatagatc tttttcaaat
    48421 tatttatttc aagcgcattt gccatttatt tcctatctcc ttatagacat gattcttaat
    48481 tgtatcatgg aatacagcag atgaaaaaac aacttggctt ccacacaaaa acacacctgc
    48541 taacaaagag gagatgtgtt ttctagttca tgatcatctt cgatagaata actatctaaa
    48601 caactctgga acttttaaaa tcataaaagt accgacagca actactacga tgacatatag
    48661 aagatcctta taactgaggt tcttgtcttt tttattcatg ttaagctttc ctccgtcctt
    48721 ttatcgaaag cttttttctt ttattttaac cgaaataaat aaaatggaaa tagttatttt
    48781 caatttctgt tttcgctgtt tgttttacgg agaactttgc catggaacgt gcgtctcgtc
    48841 gctgatgcta agttttttcg ataaaagctg ctagaaggaa gacagcgtgg tttattttgt
    48901 atcgtctttt gctaaatctt ccaagatcat ctggacagca gactcgttcc cattgaattc
    48961 tgccactttt ttggcatcaa tgtacttctt ttgataatcg tctttttgga cccacgcatc
    49021 atgcttcgtt tgataaagtt aatcaaacgg ataccactga cgctgttcat cgaacaacaa
    49081 actttctttt tgggcctgtt caactatgga tttaggataa ttcaaaactt tcagcaaggc
    49141 aatcgcattt cgggtccttg aaggtccttc ttttaatttg aaatcaaaac tgattccttt
    49201 tccttctgtt acttgttctt caaagtggac attctcacaa taattcttta aaatctctgt
    49261 caattcgata tcatgtgtgg cgacaaatgc taaagatgga tactccgcaa gccaccgaac
    49321 gactgaagcg gaagaagcaa tccgttcgat cgtatttgtt ccttttaaaa tctcatctac
    49381 gaagcagtag cagcgttctt tggaagccac ttggtccagc aaacgtttga tcgatttgat
    49441 ttctgaaaca aaataactgt cgccttcaaa taaatcatct tcgattgcca tggaagtcaa
    49501 cacatgtcca ggttgcaggg taaactgttc tgcaatcgct gtttggatcg tctgtgctag
    49561 aatacagctg atggctatac ttttgacata cgtagatttt cctgatgcat tggaacccgt
    49621 gactaatgta ttcttctgcc agttgactgg attaactacc gcttctttta acaatggatg
    49681 atagatatct ttcgctgtca caccgccctc agtaaactct ggtcggcaag tgatcggcat
    49741 ataggtgcga taattcaaaa cggctgctgc aacttccaat tctcccagca attcccacag
    49801 cttgatggct tcagttgaat gcttttctaa tttagtgaga acgaaatgat aggagataaa
    49861 tggcagcatg aacatgatgt tgacatattc aaaaatcaat tctgcttcac tcccgctctt
    49921 cattcgaaat gaaaaaccta atttagggat gtctcttaat ggtttagttt gctgtttcag
    49981 ctctttttga agtggtgtcg tcatttttgc taactgattg gcggctgaaa tcgttgaaac
    50041 caaataactc atgctattca gctctgtttc tagtgtttgt ttcttcacta gataatagac
    50101 cgtattgaat actaagccac cgattgctag atagattcct agtacttgcc ctaaaagaag
    50161 caaacctaca cccaccagcg gaagcaagcc aagaaaaata aataaaccta cgcgtccaat
    50221 ctttttactt tttccatccg ctagatattg ttttgcaaaa ttatgatctt tttttcctaa
    50281 acgagcgaat tgatattgga ttctttctct cagttgagga ttttcctgat aaaaagcaat
    50341 tagtttttct aactgataat cttctccaaa atcaaagcta cgtaaacgct gatacaacgc
    50401 ttctgaacct acactagaat aggtactgtt gatcccttca aatacttcga acatatccaa
    50461 gtcataccaa gtaagatcgt cgatttcact atcccatgaa cggaattttt tctccgtgag
    50521 ccacgcatcc ttcaaacttt cttctttatc aaatctcggt tgataaggaa tttttcccca
    50581 attactacga actgtttcct ttactttcaa acgtgaaaaa atgtctaaaa caactaataa
    50641 aataacaact gcaataataa ttaaaacgat aaattctctt tggcccatat ccctctccgt
    50701 tccttgattt gttagtttta gtatagcact gttgcgctat ttgaaaaggg ggtacaaaaa
    50761 taactgtctt cgttacctaa ttcgtatcaa tcacaccaaa ataaaaacac cttccaaaaa
    50821 agaagatgtc tttaaaacga gatacaattt tttgtcattc acttcaggta aattagagcc
    50881 aattaaaccc tccgcaactt gacggagatg gagaagatgc ccccgtctaa tctagcggtc
    50941 atctctcctc ccattccttc caccaatttt tttgacaggt aaagcccgat tccgctagat
    51001 tccgtatttg tgcggctggt attttcggta taaaaacgct ggaagacttt ttcaacagag
    51061 gtttgcgctc ctggtttgat aggattggcc actgttaatc gaatgaatga atcctctttt
    51121 gaaaatgtca cctttgcttg tccatctgca taacgcaata aattctgtat gatattttgc
    51181 agcactcgca gcaacatgag cggatctgct aatacagttg tatttatcga gtgttcagcg
    51241 aacgctacct cgatattttt ctcagaaaat tgttcataaa aagagaaaaa tacttcttcc
    51301 acctctttag aaaaagaaag cgtatggaga tccatttctt tttgcttcgt ctctattcga
    51361 gcaagatcat aaaactgatc cgtcatactg agtaatcttg caacagattg tttgatgatc
    51421 gttaaatact tctcccgttt ctccgtatcc tctacctgtt ccagtaattg tacataaccg
    51481 ttaatagcag tcaaaggagt tctgagatca tgagaaaggc cgacgatcga tagtctgatg
    51541 ttttcttcca tttcttgaaa tgcttgtcct ttttgttcat tttcatccac ccatgtattc
    51601 agtgcatcta ctaggtccat tagattcttt tctcgaaatt cgactgaaag acgaccacca
    51661 tattcttgtc gttcagaaag agtggcgatt ttcttagtta atctcttgat ttccctttgc
    51721 aggaagaaaa taaaacttcc cataaagata acaacgagaa gcaacagcca cttaatcaaa
    51781 tctaaacccc actccccaca ccgtatgaat atacacagca tgtgcctttg ctagtttccc
    51841 tcgaagattg gaaatatgca tattcactgt tttctcacta tcaaaatatg gttcttgcca
    51901 aactgtttca taaagatttg ctcgcgaaaa tacttttttc ggatgttcta aaaaaagctt
    51961 cagcagatcg aattctcttc ctgtcaaatg gacagccaca ccattcacat ttacatccct
    52021 cgactctttg tccagtaaga tttccttgta atagatgcgt tgcgacagat cttttacttg
    52081 gacagaagtc tgatgacgaa gttggatgcc gattcgtaac aacagttctt cgatatcaaa
    52141 tggtttcgtc acatagtcat ccgctcctgc tgccaacaag gaaactttat ctctttgatc
    52201 gctttttgct gaaagaacga tgatcggaac attcgatgtc tgtcggattt ctgctaacaa
    52261 tgcttcacct gaaagccctg gaagcatgag atctagaagg atcaaatcga caggatatgt
    52321 gcttaacaat aattttcctt ccgtacctga ataagcatca agaagtttgt atctctgttc
    52381 aagaacctcc tccagtaaag aatgaacagc ttcatcgtct tcgataatca aaatagtttc
    52441 cagcaggaac acctacttaa tgtcagattt ttggaaaata aacattccta gagctgatga
    52501 taccacgatt gttactagac tgaccagaat cattttcggt gcttgcgaat aagttgcttg
    52561 ttgcgcaaag tccgtataca tgagccacgt ttccgtaaac cactccgtta gaaattccaa
    52621 gcctttgatt tttgcgaaaa caccgggaac gaaggccgtt cccatgatat aaagaaaaga
    52681 aagaagcaaa ctgacactgg tacttctagt gatgaatgtc aaagccactg gaacagataa
    52741 tgtcgctaat atcagcagaa gcaatacagg aaatactttt agaacataat ttccatagtt
    52801 tttgattccc tcaacttcac caaatccgaa agcaagtgtg taagcaatca tggctagccc
    52861 cgtaatcaaa accacgccaa gtgttgtgat caaaagcgta acgagaagtt tcgacacata
    52921 ataagtcatt cgattccgtc ctaaagataa cgtattgcga atcgtaccgt tttgaaattc
    52981 ttccccaata taaaagcacg caaatgctgc gaaagccaga agaatccatg cagccatttt
    53041 ttccaccatt aaaatccctg ttgcaccgtt gatgactgta tccgttgctt ggtttaattc
    53101 attgccgtat tttgtatgca tataccattc gttccaagaa taaaaaagcg gaagtacgac
    53161 catcagtcct agtgagaccc acattttacg gctcttccat acttttattt tttcagcagt
    53221 taatagatta aacatgtgga ttgcctccag ttaattgtaa gaaataatct tccagttttt
    53281 gcccaactag gtggatacca tccacaggaa cttttgcctc taccaatgct tgattgatgg
    53341 ctgctacttg gtcaattcct tcaaaaagct ggataacacg gctagattgg acaaaataat
    53401 ccctataacc aagagagtcg agaaccgtta ctgcttgttc cgtcgccgaa gtgtcaattt
    53461 ttataaattg acgagattct tgtgccaatt cttctttcga taattctttg atcaagtgtc
    53521 cttcatgcaa aataccgtag tgagtggcga tctgtgacaa ttcatctagc aagtggctag
    53581 aaagcaagac ggtgatgccc cgttctttta ccaaacgttg gatgatttct cgcatttctg
    53641 ccatccctgc tggatccatc ccattcgtcg gttcatccaa aatcaaaaat tctggattcg
    53701 tgatcagtgc atttgcaata gctaaacgct ggcgcatacc taaagagaaa ttctttgctt
    53761 tctttttccc tgtattttcc aatcgcatca ttttcagcag gtcttcgatt tctctatccg
    53821 acacaccgcc gttcgctgct tggatacgta ggttatctct tgcagttaat tctggataaa
    53881 gagcaggggt ttcaatcgat tgacccatac gtcgtcttgc tgcctgcaaa tccgtcgttc
    53941 caaataattc gatcgaacct tcatcaatcg agatcaaacc cataatagtt ctcataaagg
    54001 tagacttacc cgctccattc tccccaatca aaccatagat cattccacgt ttgatttcga
    54061 tcgatacttt atctaatgct ttggcagcac cgtatttttt ggttatacca gttgctttca
    54121 agaccgtttc atttccaaaa tccatactta tccttctttc ttattgataa gtgaagtata
    54181 gttgacgggt ataaagaaag cagccagaaa tgtataaaga aagtgtaaat tttccaattg
    54241 aatttcaaga aaaatcagac tgtctcgcag acgtggttga gttgtctgcc aatcaaagag
    54301 cgcaccaaac agtgctaatc caagtggact ggccaccatg gtaatcgttg ttatcgttga
    54361 catcagacgt ccttgtatat gcgttggaac ttctcgctga agccagacca tataaaaagc
    54421 aaaagaaaac attaatcctt aaaaacggac ttatgaaaaa taatacatgc cgtttgggat
    54481 ttacaattag ctaccaaata agaggttatg acaaaagact tttcacaatc ccttcttccg
    54541 aataaacggt gttctcctgc caactcctcg gtcacaagtc acaatattta tcaaacatga
    54601 gaagcaattt ttgtaaattc cttcttattt cttgaagctg accacttctg tcacaacctc
    54661 agaagcggat aaataatatt gtttgatgat actttatctt tttcccgttt gtttatagat
    54721 cgaatgatat tatagaccaa gaatcctacc tattaacata gctaaaaaca caaaaaaggc
    54781 actttccaaa ttgtggaaag tgccagaaaa gttaggatta aggtaactgc gctcagcatt
    54841 gcttcgccca gtacgattct cgtaactcgt gaactcgaac gataatcgca attaacgttt
    54901 tgagaactgt gaagctttac gggctttctt aagacctggt taaacaaaac tctattttat
    54961 tgcattttac aggaatgtat ttcatgcgaa attaataagt ctttgtttat agagttttac
    55021 tagggtataa aaagttgtat gttatcatta cctcaaaaca ttacctcagc ttggcgagtt
    55081 ggttgattga agaataaatc atttatctag atatctcaat aaaaaatgat ttctaagaaa
    55141 cttgtatttt cgtgttatta tttttttgcc ctatcaaaat ttctaattct tctagcgtaa
    55201 tatgattatg acacataatc gattcaatta cttcaatacc tatttttttc gagaaagatc
    55261 ccttttgttc actcacaaaa atactttcag aagacatata taaggaacga agttttggaa
    55321 ttagtatatc gtacaaatag accaccataa aatcgtcctt gcaaccattc tcaaaaagaa
    55381 ttaaatttgc tgattgtgtt acatatatca ctgaattaca ttcatggtca attccaaaaa
    55441 caatgtctac ttcattttct tttctaattc gcgcagttac atttttcaaa ctttcaccat
    55501 tacttataaa tgctttagtt actgtaagct ctgatgtttg ttcagatatt ccttttataa
    55561 tattttgttc atcagccaca ttccccataa atccgccgat tccttttaag taataattct
    55621 cacctaagag tcgcttgcat tcactgaatt gataaaaaaa ccagttaaaa aaatcttctg
    55681 gaaaaataaa ttctgatcga actttattta tatttttagc tcctattaat tttataactc
    55741 gattaatgtt cacttgctgc gatgaacata taactgaata aattgagttg tttaattcaa
    55801 aaaaacaact ttaacatatg catcattaat tcgttatttt ttaggtagat attgatcatt
    55861 gttatctctc tttttttgct tcccatactc caacttagaa attgccgtta taaaatttat
    55921 tttaaaggtg actttttccc ctgaagtatc ggtagtagga atacttaata cctcagcttc
    55981 tgaggaggta tcccaattat agttatactt tgcatcatca ccataaagaa caattccatt
    56041 ttttttatct tgtgccgtta gctttactgg ttcgcttact tttcttgtta atatcttgga
    56101 ctttacatca tcgatattgc ttattttatc aagcttaaat aatttacaaa ttaatttcgt
    56161 actagttttt atatttgacg agagtgtaaa atattttgtg taaatagaaa aaaggaagtc
    56221 ccttctgtag aatagagtta ccacaacaca ttcacagaaa agaggacttc catatgaacg
    56281 attttactac agaaattcta aagactctag cgaacaaagg cgatttgaat gaattattcc
    56341 gtgtccattt ggaaaaagct gtcaatacgc ttctc
//
LOCUS       DAMEET010000004        56349 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.004, whole genome shotgun sequence.
ACCESSION   DAMEET010000004 DAMEET010000000
VERSION     DAMEET010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 56349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.004"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            290..1135
                     /locus_tag="PB780_000229"
     CDS             290..1135
                     /locus_tag="PB780_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDE0732307.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1416..2636
                     /locus_tag="PB780_000230"
     CDS             1416..2636
                     /locus_tag="PB780_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HDE0732308.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2633..5353
                     /locus_tag="PB780_000231"
     CDS             2633..5353
                     /locus_tag="PB780_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDE0732309.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5337..6281
                     /locus_tag="PB780_000232"
     CDS             5337..6281
                     /locus_tag="PB780_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDE0732310.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6350..7360)
                     /locus_tag="PB780_000233"
     CDS             complement(6350..7360)
                     /locus_tag="PB780_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDE0732311.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7540..7884)
                     /locus_tag="PB780_000234"
     CDS             complement(7540..7884)
                     /locus_tag="PB780_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDE0732312.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7877..8311)
                     /locus_tag="PB780_000235"
     CDS             complement(7877..8311)
                     /locus_tag="PB780_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HDE0732313.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8449..9186
                     /locus_tag="PB780_000236"
     CDS             8449..9186
                     /locus_tag="PB780_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDE0732314.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9179..10390
                     /locus_tag="PB780_000237"
     CDS             9179..10390
                     /locus_tag="PB780_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDE0732315.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10594..11376
                     /locus_tag="PB780_000238"
     CDS             10594..11376
                     /locus_tag="PB780_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HDE0732316.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11446..12108
                     /gene="trmB"
                     /locus_tag="PB780_000239"
     CDS             11446..12108
                     /gene="trmB"
                     /locus_tag="PB780_000239"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDE0732317.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12206..13960)
                     /gene="ade"
                     /locus_tag="PB780_000240"
     CDS             complement(12206..13960)
                     /gene="ade"
                     /locus_tag="PB780_000240"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDE0732318.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14092..15021
                     /gene="tadA"
                     /locus_tag="PB780_000241"
     CDS             14092..15021
                     /gene="tadA"
                     /locus_tag="PB780_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HDE0732319.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14978..16027
                     /locus_tag="PB780_000242"
     CDS             14978..16027
                     /locus_tag="PB780_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HDE0732320.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16024..16302
                     /locus_tag="PB780_000243"
     CDS             16024..16302
                     /locus_tag="PB780_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDE0732321.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16277..16747
                     /locus_tag="PB780_000244"
     CDS             16277..16747
                     /locus_tag="PB780_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDE0732322.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16764..17066
                     /locus_tag="PB780_000245"
     CDS             16764..17066
                     /locus_tag="PB780_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732323.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17083..17454
                     /locus_tag="PB780_000246"
     CDS             17083..17454
                     /locus_tag="PB780_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HDE0732324.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17426..17785
                     /locus_tag="PB780_000247"
     CDS             17426..17785
                     /locus_tag="PB780_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732325.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17957..18964
                     /locus_tag="PB780_000248"
     CDS             17957..18964
                     /locus_tag="PB780_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDE0732326.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18989..20173
                     /locus_tag="PB780_000249"
     CDS             18989..20173
                     /locus_tag="PB780_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDE0732327.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20610..22193)
                     /locus_tag="PB780_000250"
     CDS             complement(20610..22193)
                     /locus_tag="PB780_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDE0732328.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22771..22914)
                     /locus_tag="PB780_000251"
     CDS             complement(22771..22914)
                     /locus_tag="PB780_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HDE0732329.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23137..23616)
                     /locus_tag="PB780_000252"
     CDS             complement(23137..23616)
                     /locus_tag="PB780_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDE0732330.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23689..23973)
                     /locus_tag="PB780_000253"
     CDS             complement(23689..23973)
                     /locus_tag="PB780_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732331.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24051..24245)
                     /gene="ssrS"
                     /locus_tag="PB780_000254"
     ncRNA           complement(24051..24245)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PB780_000254"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24317..25603)
                     /locus_tag="PB780_000255"
     CDS             complement(24317..25603)
                     /locus_tag="PB780_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDE0732332.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25741..26412
                     /locus_tag="PB780_000256"
     CDS             25741..26412
                     /locus_tag="PB780_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HDE0732333.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26378..26866
                     /locus_tag="PB780_000257"
     CDS             26378..26866
                     /locus_tag="PB780_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HDE0732334.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26879..27826
                     /gene="prmA"
                     /locus_tag="PB780_000258"
     CDS             26879..27826
                     /gene="prmA"
                     /locus_tag="PB780_000258"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDE0732335.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27827..28591
                     /locus_tag="PB780_000259"
     CDS             27827..28591
                     /locus_tag="PB780_000259"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDE0732336.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28824..31037
                     /locus_tag="PB780_000260"
     CDS             28824..31037
                     /locus_tag="PB780_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDE0732337.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31048..31494
                     /locus_tag="PB780_000261"
     CDS             31048..31494
                     /locus_tag="PB780_000261"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDE0732338.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31665..32924
                     /locus_tag="PB780_000262"
     CDS             31665..32924
                     /locus_tag="PB780_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HDE0732339.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33013..33960)
                     /locus_tag="PB780_000263"
     CDS             complement(33013..33960)
                     /locus_tag="PB780_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDE0732340.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFHIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34354..35646
                     /locus_tag="PB780_000264"
     CDS             34354..35646
                     /locus_tag="PB780_000264"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDE0732341.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35661..37430
                     /gene="aspS"
                     /locus_tag="PB780_000265"
     CDS             35661..37430
                     /gene="aspS"
                     /locus_tag="PB780_000265"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDE0732342.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37539..38414
                     /locus_tag="PB780_000266"
     CDS             37539..38414
                     /locus_tag="PB780_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDE0732343.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38680..40563
                     /locus_tag="PB780_000267"
     CDS             38680..40563
                     /locus_tag="PB780_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDE0732344.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40638..40922)
                     /locus_tag="PB780_000268"
     CDS             complement(40638..40922)
                     /locus_tag="PB780_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDE0732345.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40912..41646)
                     /locus_tag="PB780_000269"
     CDS             complement(40912..41646)
                     /locus_tag="PB780_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HDE0732346.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41775..42398
                     /locus_tag="PB780_000270"
     CDS             41775..42398
                     /locus_tag="PB780_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDE0732347.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42590..43711
                     /locus_tag="PB780_000271"
     CDS             42590..43711
                     /locus_tag="PB780_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDE0732348.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43718..46846
                     /locus_tag="PB780_000272"
     CDS             43718..46846
                     /locus_tag="PB780_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HDE0732349.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46865..50059
                     /locus_tag="PB780_000273"
     CDS             46865..50059
                     /locus_tag="PB780_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HDE0732350.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50205..50912)
                     /locus_tag="PB780_000274"
     CDS             complement(50205..50912)
                     /locus_tag="PB780_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDE0732351.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            51677..52075
                     /gene="spxA"
                     /locus_tag="PB780_000275"
     CDS             51677..52075
                     /gene="spxA"
                     /locus_tag="PB780_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDE0732352.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            52407..53060
                     /locus_tag="PB780_000276"
     CDS             52407..53060
                     /locus_tag="PB780_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HDE0732353.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            53286..54284
                     /locus_tag="PB780_000277"
     CDS             53286..54284
                     /locus_tag="PB780_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HDE0732354.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            54460..56268
                     /gene="pepF"
                     /locus_tag="PB780_000278"
     CDS             54460..56268
                     /gene="pepF"
                     /locus_tag="PB780_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDE0732355.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT        18537 a         9724 c        11681 g        16407 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      841 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      901 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      961 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
     1021 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
     1081 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
     1141 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1201 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1261 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1321 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1381 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1441 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1501 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1561 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1621 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1681 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1741 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1801 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1861 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1921 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1981 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     2041 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     2101 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     2161 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2221 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2281 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2341 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2401 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2461 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2521 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2581 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2641 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2701 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2761 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2821 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2881 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2941 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     3001 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     3061 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     3121 agaatattat cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa
     3181 tcaaaaatta aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga
     3241 agcttatttt ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt
     3301 attggcacaa ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa
     3361 tttttcttta tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga
     3421 tgagcaactt cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg
     3481 tttggaagaa ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag
     3541 atattatttt tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac
     3601 agcggaacaa ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc
     3661 agaattgaag attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt
     3721 tttatatgaa gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga
     3781 aagccagatt tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga
     3841 agtgaatgac ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc
     3901 agtctcttca tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat
     3961 atttgtacca atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta
     4021 tttcttctac aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg
     4081 aatgtggcaa gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga
     4141 agaaaaacaa aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt
     4201 tgcggatcat cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca
     4261 ggaagtcata gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca
     4321 ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt
     4381 accgatacaa gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga
     4441 gatggaacag atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat
     4501 caatcaattg aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca
     4561 agcaggctta tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga
     4621 acagcagatg aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga
     4681 gctgacaaaa gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact
     4741 ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga
     4801 gttgcaaaga gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa
     4861 attacaggaa ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga
     4921 tctttcaaca gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta
     4981 tttaggtatt ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac
     5041 tttaacagac ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca
     5101 gctgattatg gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc
     5161 gatcatcatt gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga
     5221 acttttagct aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat
     5281 ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga
     5341 aaaaaattcg tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg
     5401 cagatgtaag aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt
     5461 caggtactat cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag
     5521 ccggcacagt cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa
     5581 agattcttca tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg
     5641 aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga
     5701 tcaccaatgc gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat
     5761 tttttgagtt tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc
     5821 atactttgac aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc
     5881 gagcattgtt gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg
     5941 gtcctatgtc gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg
     6001 acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc
     6061 tcgtattacg tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac
     6121 gtccaagaat aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc
     6181 agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg
     6241 gtatggacaa ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca
     6301 ttcaaaacaa aaaaagcgag acataagtct tcgctttttt tgttttcttt tattttgatg
     6361 aatcagttgt ttcttcagtt gaagattctt ttgttgaatc agttgatttt gtgtcagaac
     6421 tctttgttga agctgtagtt tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg
     6481 cactcaacac attttcaaag gcatcatctt tgatttttac atttgcatct tttaattctt
     6541 caccgattac tttagttgtg aatgcattgt cacttagttt agtttcagta gcgatatctt
     6601 ttagttgatc tttgtattta tccatgtcat tgcctttatt ttggtttttc accattttga
     6661 caacgtagta ttctgtcgca taagaagtag gatttgttgt agtaataaca tcggagatct
     6721 caccgtcttt caatttgaaa gctgcttctt ttacttctgc aggaatagtt gttgtcgttg
     6781 agtcgaattt aactttaccg ccatcttctt ttgtctctgt atcagttgat ttgtctttgg
     6841 ctaatttaga gaaatcatct ccatcatccg ctgatttttt cacatctttt gcttcatctt
     6901 cacttgataa tttgatgatt tgtgcttcta cttctggatg gaaagatttc caagcagttt
     6961 tcaaatcgtc atctgtaata tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt
     7021 tacgaatata ttctttataa gtatctttcg tatatccagc agcttctaat tgtgattcaa
     7081 aagtatcacc taatgatttc gcttgtttgt cgtattctgc atccacttgc ttatcggtta
     7141 ctttatcgcc atatttgtta ttgaatactt tataaatgat catccgttgt actaaggatt
     7201 ggttcgatga ttcaagtttt gcttcatcat agaaatcaga tacagtgatc gttccgcctt
     7261 tcatcgttgc gatatcttta ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca
     7321 acgcactagt agctgccaaa ataattgatt tttttttcat taaaatttgc actcctgtaa
     7381 ttagttatta tttttatata ttaactatac catatcccat ttcctttaaa aacgttgtcg
     7441 tggtagtttc aaacaaaatt caaaatttca tcacattcct aggaaatacg caaacttttc
     7501 tcacttatat agttaaatct gttgagaaaa taatcattat tatatttgtt caggatactc
     7561 tggcagatca gcttttagtt tttctacttg ttcattgatt tgtgtgatac gtggttcggc
     7621 ttggaattta aaatcttcga tatctttttt gattgcttct tggaaagcag gaatcacaga
     7681 ttcagctgtt tcttttgttt cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa
     7741 atcatttgtc agagttgttg cttcttccaa ttcttcgatc agtttctttc ttgtttcatt
     7801 cccgctcctg ggcgccaata acagacctcc gacagtgcca gcagcagctc caaataacag
     7861 tcctttgaaa aagtttttaa tcatcgcagg acacctgctt tacgattgtt tcttgaatag
     7921 cttttagttc ttccgatcca taactatctt gatgattgac aaagtgaata gaaaaatcat
     7981 cctctttgct gtagcgaggg actaaatgga tatgtgaatg aaaaacagat tgataggcta
     8041 gttcacggtt gttattcaag atattaagac ctttcatttc tggaaaagct ttttccaatg
     8101 cttgagcaat cttaggcaca cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa
     8161 aaatatctgc tacatgacgt ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga
     8221 tgtctaaaaa agcatagacc ttgtcgtctt catatatttt atagcttggg atttcttgat
     8281 tgatgatttt gcaaaaaata cagttttcca tataaaacat cctttctata aagaataaat
     8341 acttcagctt aactttacca taagttaaag ctattgtaag gaaaaaaaca aaataccgtc
     8401 attttcttcg tggtataata aataaaaata aatggcggag gaaaaagaat gagtttaatt
     8461 atcgatcatg tcacaggggg gtacggacat ctccctgtgt taaaagatat cagtttcgat
     8521 gtacagcctg gagagatagt agggttgatt ggactgaacg gagcagggaa aagtacgacc
     8581 atcaagcaca ttatcggtct gctggaagct tcaaaaggaa cgatagaaat caatcatcta
     8641 tccttaaaga aaaatacgga agaatatcgc aaacaaattg ggtatattcc agagactcct
     8701 tctttatatg aagaattgac attgagagaa catatcgaag tcactgcgat ggcctacgat
     8761 atacctatgg atatagcaat gaaaagagca gaaaagttgt tggagacttt tcggttatct
     8821 aatcgtcttg actggttccc agctaatttt tcaaagggaa tgaaacagaa agtgatgatc
     8881 ttatgcgcct ttttgatcga gccttcttta tatatcatcg acgaaccatt tttaggcttg
     8941 gatccactag ctatcaatgc attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc
     9001 ggtattttga tgtctacgca tattttagct actgcagaaa aatattgtga tcgttttgtg
     9061 gtcttacata acggagagat acgagcagac ggttctcttg agatgttgag aaaagaattt
     9121 cagctgccgg attcttcttt ggacgaaatc tatattgctt taacaaagga gcaggcacat
     9181 gagtagtttt tttcaaaaac gtttagcaag acatcaaaag aaaatgatga aatatatgcg
     9241 ttatgtattg aacgatcatt ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt
     9301 atattattct aattggttga aaaccctgca gccaccgttt gcaattggag ggctcataat
     9361 ctctgtattt tggatgctct gtttattcgt gggtaagatg gctacttttg cagaggcagc
     9421 agatattgtt tttttacttc ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt
     9481 tcgctattct tgcatttttc cgttgatctt tttgatattg gcatgcggat tggccatgcc
     9541 attagtggtg gttagtacgg agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt
     9601 atggagtttg aaattcagtc atctgcaaat caagagagca gaattgtttc gactagatca
     9661 acaagttatt cgttattgga aattaggctg gtttttttct tctctgatct ttttgtttct
     9721 atcattttat tggctaatat gggcaggaat agcaggtgca ttagtacagg caagtctata
     9781 ttactggttt ttgtggaagc aacaagaaaa aggattggat tgggagaaaa tggtgcatgt
     9841 agaagaacat aggttgcatc agatctatca atttatcaat ttattcacag atgttcctga
     9901 aattacggca aagatcaaaa gaagacgcta tttggatcct atattgactc atattaaaag
     9961 agaatcaaaa aacacttatc tttacttgtt tgcaagaaga ttggcaagag gttctgattt
    10021 tagtggtctt tatttccgat tagtaattat aggtgggtta ttgattgccg gagtagtcga
    10081 cttttatttt agtctgatta tcggtttctt atttttatat ttgatcggtt tccaactgtt
    10141 acctttagcg aaccagttcc gctatatggt tctcgttcag ctttatccaa tccataagaa
    10201 tcagaagaag caggcgattc agtatctact cacatgggta ttaattatcg cgtcggttat
    10261 ttttggtttg attgctgcag tcgtattaga tgggcagcag cgatgggttc cgctagcggc
    10321 ttatttgatt gttactatgc tctttgttaa actatatgta ccaactcgtt tgaagaagat
    10381 gtcagactga aaacaaagct gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca
    10441 gtaagaatgc tgttcgttct attttttttg agaaaagctg gttttttgaa tggcaattaa
    10501 atgaaaaaga acgtatatgt gcttgacgtg tagtgttcag gcgactagaa taataagtac
    10561 tgcaaaacaa agagtaagag gacgggagaa tgcatgatgg agtttcagtt agaccaagag
    10621 tggcggttac gtcccatcaa aggcgacaca ggaaaaacat acataggcat gcgtgatgag
    10681 gacaaagtct ttattaaacg aaacacgact ccgatgttgg ctgctctttc aagggaagga
    10741 attacaccaa aacttgtttg gacaaaacga acggggaatg gcgatacttt gacagcacaa
    10801 gagtggctgg aaggtcgctt gctgactcct aatgagattg gtcaaagaaa cgacgtgatt
    10861 gatgttttgt accatttaca tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt
    10921 ggtcgtgtca tgacgccgga gaagatgctg caaaaatatg aaaaagaatt accggaagaa
    10981 ctccagaaaa atcaatttct gcaaattgtc catcgttttc ttcgtgagca gatcccgtca
    11041 atagataagg aaaacttatc agtagtacac ggagatgtaa accatcgtaa ttggttagta
    11101 tgtaaaaatt atttatatct agtcgattgg gattcaatca tgtttgcaga ccctgcggtg
    11161 gatattgcaa cgattttagg aaattatgtg ccactgtcta gctggaatca gtggctgatc
    11221 agttatggca tacgcccgac tgacgaaatg cttgaaaaat ttcattggta tgcggccatg
    11281 aatttgctcc aagaaatcac cagatattgc aaacgtgaag atcatcgacg gatgaacgaa
    11341 gaaattttac aattaaaacg aattttcagt ggctgaggtt tggcccaaac tctgcggaga
    11401 tttggccaga cctttttaat tagaggaaag gaatggagat aaataatgcg tgtccgaaat
    11461 cgtccgggag cagcggaaat gttagcggct cacccgaatt ttgtaattag tgatccaaca
    11521 ttgtggaagg gaaaatggaa tgaattgttt gaaaacgatc atccaatcca cattgagatt
    11581 ggtatgggga aaggccagtt catcactgga atggcaaaag cccatcctga aatcaattat
    11641 attggagtag aaatgcaggt cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa
    11701 ccgcttccta acctgaaact tctacatgtg gatggatcag cgttgacaga gtatttcgct
    11761 gacagcgaag tagatcaaat ctatctgaat ttttccgatc catggccaaa aaaacgtcat
    11821 gaaaaacggc gattgacata taagactttt cttgctgtag atgaacagat tcttcgtcca
    11881 aatggagaaa tccatttcaa aacagataat caaggattgt ttgagtattc tctggcaagt
    11941 ttttctcaat atggcatgat cctcaaacag gtatggttag atttacatca aagtcaattt
    12001 gaaggcaata ttatgacgga gtatgaagaa aaattttctt caaaaggtca acgtatttat
    12061 cgagtagaag ctcaatttca agataagacc aaaaagaaaa gtctgtgaaa ctaactttct
    12121 gctttcataa aaacattaat atccataaat aaatcccaga aaatgaacat tacagatgta
    12181 atgtattttc tgggatttat ttgatttaaa atgtatttat ttcatcggtt tttccacaag
    12241 gtggaatcaa ttggaagcga gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg
    12301 gtatgacaga taatgttaag aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc
    12361 ctagttgatg tgctttagtt attaattcat tcaactgttc atagacgatc gaaaaaggtt
    12421 cctgtgtcat gattccgcct ataggaagag aaacacaagc tatttcctgc tgtttgttga
    12481 caacggtcat cccgccacct ttttgaatca aaagattagc agcatacagc atatcctcgt
    12541 cgtttgtccc aacgacaact agattatgcg agtcatgggc aatcgttgtc gctatcgctc
    12601 cttgtttcag ctgaaatcct ttcactatgc ctaatccaat gtttcccgtt tgatggtgac
    12661 gctcgatcac cgcaatcttt aattggtctt tttcgattga aggaatgaat cgttcgcctt
    12721 gtcggtccac tttttctacc aaatctttag tcactagact attcgggata atttcgatca
    12781 catgggcttg tgaagaagaa agaagcagct ctaacgaatg tggatttaat ggttgagcgt
    12841 ttagttttgg cagacaacac gtatatgaat ttgaatgatc agcaaataaa tggtcttgga
    12901 gttgcccaga ttgaacaact aatgttccct tatagaaaac ttcatgaatt ggcagcttgg
    12961 taagatcgtc tgttaagaag aaatcagctt gatacccagg ggcaatcgca cctagtgttt
    13021 tcaaatcaaa acattctgct gcattcaatg ttgccatctg aatggcttgt aatggatcga
    13081 ttccaagtgc aatagcttga cggacgatat gatcgattga tccttctttt actaaatcat
    13141 tgatcagttt atcatcggtg acaaagaagc atcttcttga attttcaggt gtgatcgccg
    13201 gtagtagcga tgccagatct tttgcaacgg tcccttctcg aaccattaaa tacataccaa
    13261 gatccaaacg ttctttagct tcttttgcag ttgtagcttc atgatccgtc cgaatacctg
    13321 ctgcaggata aacatttaac tcttccatgc cgataccagc tgcatgcccg tcaatttttt
    13381 tcttatgctg gtgcatcaat tggatctttt ctaggatgtc tgtttcatta tttgctactg
    13441 cttgatagtt catgacttca gctaagccta aaacttcggg tttttgtaaa aaaggggcta
    13501 atttttttgc atttaaattt gcaccattgg tctcaaaagg ggttacaggt acacaagaag
    13561 gtagcataaa aaagatattc atgagtgttt gtcttgcatc ttctatcata aactcaagac
    13621 ctgtactccc agcaatgttt cctatttcat ggggatctgt cactacagta gtaactccat
    13681 gaagcaagga tacttttgaa aattcgcttg gtgataaaag tgagctttcg atatgcacat
    13741 ggccatcgat gaatcctggc actagatatt gcccattagc attatgtacg ctttttcctt
    13801 cgtattcacc gattcctacg atcattcctt cgcagattgc tacatctttt tccatgattt
    13861 ctttcgtaaa gacgttgacg atcttagcat gcttgatcac taggtcagca ggcttttttt
    13921 tacttgctac ttcgatatat ttccttaatg ttgtttccaa tggagtgcct cccaagataa
    13981 atttttataa gtataacata aattcggttt ttgccaatat ttttttgtaa attcatttta
    14041 tttgaaacaa aaagatcaaa aagacgtatg ttttttgtgg aggggagaat aatggaaata
    14101 aaacgattat cggatcaaat gataagtgaa agttttgcca aacatgccca agacgtatat
    14161 atattgccag cttcagaagt attttacatc tattttcgct atggaaaaaa acgaacattc
    14221 tggaagaaag tctcttcggc agatggacag caactgatct caagatttaa gtatttggga
    14281 cagatggata tcggagaaaa aagaaaagct cagttaggtg catttactta ttctttagaa
    14341 aaacaggaag ttcgtctgcg gttgtcttct gtaggagatt accttcaacg agagagttta
    14401 gtgattcggc tattatacgg tacttttgaa acaccccatc gttgctttag ttctcgagat
    14461 tttgcaacta ttttacagaa aaccgtcaga cgtggattat accttttcag cggaccagtc
    14521 ggatcaggca agacttctct aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt
    14581 actatcgaag atcctgtaga gatagaagaa ccgttttttc tgcagctaca gatcaatccg
    14641 aaaatagcac agacttatga tacacttttg agactcgcat tacggcatcg tccagacttg
    14701 ctaatcattg gcgagataag agatgaatca actgcacatg ctgcggtaag agcagcacta
    14761 acagggcacc gggtttttgc aaccgtacat gcacgcaatc ttgaaggtac attggtcaga
    14821 atccatgaac tgacaaagaa aaaagaagag ctatctgaat gtttagctgg tgtcatttat
    14881 caagaacttt tggaagatgc caatcaccat ccttctgttt tatggagtta tcaatttatg
    14941 gaggaagaat cagtaaaaaa atcatgggag gaaagttatg aagaagctca ggaaaatatt
    15001 tggcagtcag aactattcta aaggattaac aagaagccag caaaaccagt ttgcacaaac
    15061 catgggagaa ctgcttttat ctggttttag tctgcaagaa tccctttatt tattactcac
    15121 gatccgttcg tttccacctg ttattttggc aaatatccaa catcagctaa aggaagggca
    15181 agcgtttagt gaggtacttt cgcaaatagg atatggagca gagcaactgg tacagatcga
    15241 gttagctgaa cgtcatggga atctgccgac aactttgttg gaaattgcca aacagtttcg
    15301 gcttgttgaa ggatttcgca atgatttgaa aaagctgctt gcatatccga tgctattgct
    15361 cacctttttg ctaggtatct tgtttagtat gcggttgttc gttttgcctc agcttattgg
    15421 ttcaggaatg gtcatgcaag agcattgggg tattcagctg atacaagtct ttcattggta
    15481 tgttctacta ttcatcctag cggttactct attggcttta ctagtaaggt atcgtctgat
    15541 gaagttgtct cctgttgatc agagtgaatg gataagcaga caattattga ttggaccgat
    15601 atattccttg tatcaatcgg cttatttgtc actagagatt ggaaagcttt ttcatgaagg
    15661 tttcgaatta aggcaaatca tagtctgctt aaaggaaacg aaacaagaaa gtctgattca
    15721 gcatcttgcc tctaagttga ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt
    15781 ccagaattat cgttttctat cagaagaatt cagtaaaatt gttttacagg gagaggcaaa
    15841 gggaagttta ggtaaagaac tgatttttta cagcgaatgg acaagaaaac agcttttcaa
    15901 acgtcttcat cactggatgc attttattca gccaatcatt tttttgggcg tagcggcttt
    15961 gattttatta gtttatgccg ctgttttatt acctgtttac ggaagcatag aggaggtttt
    16021 gccatgaaaa agagaaaaat atcttattat agcggtttta cattgatcga aatgctaatc
    16081 gtcttactga tcatcagtgt attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg
    16141 aatcatgtag accaagaaag tcgtgaagcg atcatccagt tggtagacac acaaaaagag
    16201 ctctatgcgc ttcaaaataa tggaagagtt cctactgtgg aggaattgtt gaatgaaggc
    16261 tatatcaaac gagaacatgc agaaatctat cagcggcctt gaaggtaagg ttttagcatc
    16321 aggatatacg ttgtttgaat cactcttact gattttatta cttacaacga tcctttcttt
    16381 tccagttctc gcattttctg catggaagaa tgaattggca gtctatcaat ttttcggcca
    16441 atttgaaaaa cgaatctatt ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg
    16501 cttttattgg aatagtgaag agaatcaaat catttttcat gttccgaatc cagataaaat
    16561 aaattgggaa gtattagata ttcctgatga gattaccttg aaacgccatg cttctattac
    16621 ttttgctgct agaaccggga atgaaagttc actaaaagct tatcaatttt attgggaaga
    16681 aaaagagcgg acgattacgt atcaattcca gatagggagt ggtcgctata caaaaagaat
    16741 tgaataaaac aggatttgtt ttgttggagt cccttacagc gcttatgatc gcttctgtct
    16801 gtgtctactt agtgagcttt ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg
    16861 aggaactgct atttattcga atgatttatg aagatatcaa gacgcagcgg atgtatcaca
    16921 aaagaccaga gcgaagggaa gaaatgatcc catacgatat ccaaattgtc gataataatg
    16981 gagtaatcga aaaagtgttt gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa
    17041 gtaattccag catttacgct gattgaatgt ctaattggac tgatgatatt gagcagtcta
    17101 ttgctcacat tcaatctttt gattcagcaa accatccaag taagtgaatt tttgcagcgt
    17161 aaagatcaaa aagagtggct aatattttta gcacaattag aagaagaact caagagcagt
    17221 cacaatgtct ctgtgaaaaa tcagcaactg cactacacga tcgaggataa acagtatatc
    17281 atcgaaagat atcaggcaat gatacgtaaa agaagaactt ccggcggaca tcaacctatg
    17341 ttaacaagta tcactgagct tcaattattg gaaaaagaac acacaatttc tttttatgta
    17401 cactttgaaa atggagaaga tggatatgcc atatggacaa aaaacgatca gtagaaaatg
    17461 ttataagggc tctattttat tttctgtctt gcttttattt cttttatgta cgtttttatt
    17521 gatggcagca ttagaaagct accgtttgtc aatggatctt aatatacgaa tcaaaaatta
    17581 ttatatagca aaaataatga agcagatggc tttatcggaa cagtctacgc ttgccgaaag
    17641 ttcggaagga gttttttact acacaactgg atctgtaaca tatcagtggg tacaacagtc
    17701 cctattctta gaggtggaag tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa
    17761 tgatacagat actagtacag aatgatggaa tgttcgtgaa aacgccctct tttctgttat
    17821 aacactgttt ttaataaaaa aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat
    17881 tgaaattttg tcaattgttc gatagaatta aacaggtgaa aaaattgtta taatatatct
    17941 agaaatgagg tgcaccatgt taccagaaaa aatggaacaa gggtttaatc aaaacctgga
    18001 agctattcag cttttacaga atgcattggg aacttccttt ttagaagcct atgtcgaaaa
    18061 tgcagaaaat ttaatagatg attatcaagt acgtgtggtg gatggagtgc cgacaaaaga
    18121 gacaacgcaa cggatcacag ctctttatga agagttgaag aaactttctt tcgagccaga
    18181 agaatggcgc agactgtctc aattactttt attgaagggc agccagacag aacatttgca
    18241 ggcgaaccat caactcactc ctgatagtat cggtttttta tttgtttttc ttattgaaca
    18301 actatacaca aataaaaaag caccggttaa gattctggat attgcggcag gaatgggcaa
    18361 cttgttatta acagttcttt taaacctatc gaatgcgggg taccaaacag aaggaattgg
    18421 tgtggatatt gatgatacat tgttggctgt cgcagcttct acaagtgatt tgacacaagc
    18481 aaatgttcaa tactttcatc aagacgggtt gcaggaatta ttaatagatc ctgtcgattt
    18541 tgcaatcagc gatttgccaa tcggttatta tccgaatgac gaaaaagcaa aagagttttt
    18601 aacaagtacc gaagaaggcc atagttatgc acatcattta ttgctagagc aatcaatgaa
    18661 atatgtgaag ccggatggtt ttggtttgtt tttgatgccg agtggatttt tagaaacgga
    18721 tcaaagtgaa gaaatcaaaa aatggtttaa agaagaaggt tatcttcaag gaatgattca
    18781 attgccagat gaattgttcc gtaataaaca atcgcaaaaa agtattttga tcttacagaa
    18841 aaaaggacca caagcacaac aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa
    18901 agaaccagag aaggtgacgg aatttttcaa cgagtttaaa aactggaaat catctgctct
    18961 gtaatcattc aacgagaagg agagtcatat gtctaaaaca attgcaatca atgctgggag
    19021 ttcaagtttg aaatggcaat tatatcaaat gccagaagaa accgtagttg cgaaagggat
    19081 cgtcgaacgt atcggtttac aagattctat ttttaccatc aagtacggcg ataatcaaaa
    19141 attcgaacaa atcatggata tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca
    19201 gttgattgaa ctgcaaattt taagctctta tgatgaaatc accggagtag ggcaccgtgt
    19261 tgttcacgga ggagaaactt atggggattc agtagtgatc gatgagaaag taatggaccg
    19321 cattgctgaa ttagcagaat ttgctccttt gcataatcct gcaaacttaa tggggatcaa
    19381 agcgtttaaa aagattttac ctgacatttt gagtgtggct gtattcgata catctttcca
    19441 ctcaacaatg ccaaagcaaa attatttata tagcttaccg gtgtcttatt atgaagattt
    19501 caaagtaaga aaatatggct tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc
    19561 aaaattattg aacaaaccga tcgaagaact aaaaatcatc acttgccact taggaaatgg
    19621 tgtatcgatc acagcagtcg atggaggtaa atccatggat acttctatgg gattcacacc
    19681 attagctggg gtgacaatgg gtacacgttc aggtgatatc gatccagctg ttctcccata
    19741 tttaatggaa aaattagaga ttgatattga tgaaatgatc aatgttttga acaagaaatc
    19801 tggtctgcta ggcctaacag gattgtctag tgatatgcgt gatcttgaaa agaactatga
    19861 aaaagaacat attcgtttag cttatgacat tttcgtggac cgcatccgaa aatacatcgg
    19921 cggttatgtg acaacgatga atggtgttga tgcaattgtc tttactgctg gtatcggcga
    19981 gaatgacgga catgtacgta gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat
    20041 cgatccagcg ttgaatgaat tgcgtggtga agaagtagat atctctacaa aagaatcaaa
    20101 agtaagagta ttagtgatcc caacagatga ggaactaatg attgcccgtg atgtggagcg
    20161 tctgcgcggc taagccaaag cattcgttag gataaaagat gaaacgcatg caagagggaa
    20221 ttcttgcatg tgtttttttt gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc
    20281 cttctttttt acaggatctt cttagggaat tttgcttgga aaagtggtca aaagggaaaa
    20341 gactggcatt ttatttattg attttcagga aaatcccaat attttatttt cattcatctt
    20401 tataagggac attacatatg taaaaagaaa aaacattttt agatcagcct cgtgggctgt
    20461 cgactttatt cttcgcttta tgtttagcta gaaaaccgat ccgaaacatt cattgatcga
    20521 ataataaaaa cttgattttc cacctgtact ttgcctgaat cgtgaaagca caggtatgaa
    20581 aatcaagttt tttgttgcga aaaaacgtcc tagtcaatca gttggccgag cggctggcgg
    20641 ctgatctgct cctgatcaat aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg
    20701 tcatggctga ctgctaggag cgttccttga tagtcttgca gcatttcttc aatttcttct
    20761 cgagcatagc tgtcgaggtg attggttggt tcatccaaaa tcaggacgtt gattttttct
    20821 aaaaagagtt tcagcaacgc caaacggatt ttttcaccac cggataaatc tttgatccgt
    20881 ttacccacat cgctttgata aaagccactt ttggctaatt gccgtcgagc gacttcttcg
    20941 tttccagtca atgtgatggc gtaagccagc aaacggctat cgggatcggc aaactgcagc
    21001 tgctgcggca aataaccgat ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc
    21061 ttttcctcac cgagtaataa tttgagtaag gttgattttc ctgcaccatt gtcgccgatg
    21121 atggctaaat gatctccacg atagactgca aaactgctgt ctttgaatag tacttgatca
    21181 gcgtactgct ggtgcaagtt ttgaatgatg aaaacttctt tgccagattt tccaccatta
    21241 gacaagcttt gcagccgatt tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc
    21301 aggcggcgtt ccagttcttt ggcttttttg aagaaggact cattgtctcc ttcatgtgcc
    21361 cactgacgaa aacgacgaat catgactttc aaccgctgga cttctttttg ctgcagttcg
    21421 taattttttg tcagctcagc caagcgagct tctttcaaga ctcgaaaacg gctgtagttt
    21481 ccgggatact caatcaatgc accttcttcc agttcccatg tttttgtggt gacttgatcg
    21541 agaaattgcc gatcatgaga aacgaccaga accgattttt ttgtattctg taaatagttt
    21601 tccaaccagc gaatactgtt aagatctaaa tgattggtcg gttcatcgag taaaagtaaa
    21661 tcggcatcgg ctgctaaaat tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt
    21721 tctgttaacc gtgcttcctg acggtgtgga atccctaagc cttgcaagat cgctgcaatc
    21781 cggtcttcta atacgtaacc gcctagttct tcaaagcgat gctgcagctc accgtaacgg
    21841 gccagacact tctctaagtt tgtatctggt tctgtcatca atgcttcgta gcgactcatt
    21901 tgctgtttga ggtcctgtat ctcagcggca gaatggagca agtagtggtt gacggtcgct
    21961 tcggaaactg taagttcttg ttcgaccgtc ccgatcttta agcctttttt gcgactgata
    22021 acaccgctgt cgacaccttc tctgccggtc aagatttgta aaaaggttga tttgccagtt
    22081 ccgttgatac cgactaaggc aattttttca ccagcttcaa tttgacagga taaatcctca
    22141 aataatggat tcccgccgat tgttttttta atagaaattg cttgtatgat catgatctct
    22201 ctccttgaga ccactcaaaa aggccatggt tatccatggc ctgtgttatt taaactcaga
    22261 ttatcatgag aaaaatacct tgagatggcc attgatttta cgtgaattca actgttttgg
    22321 acaaacttgt cccgtcaaca gcaactgtaa aaccaattgc atggctaaat gcgatatggt
    22381 atcgttggta attttcatta cttgttattt tagtcatgcg catcatagta ttttccctcc
    22441 ttcgtgtagt tgtcttttcc agtgtacctg aaagtgtcag aaagttcaac tagcgctgac
    22501 atttacttaa tatttatcga tgtgtaattc tgtcagagag ctttttttat ctgactgagg
    22561 gtaatgtctc gtggaccaca tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca
    22621 tatggtgttt gataaaaaac tttgtctaca tgacttcctt ttgccatttg cttaataccg
    22681 ttgcattaaa cagctcttct ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt
    22741 gtggttgcaa tggcaagcgg tagcctttca tcaattcttg aatgatttac cgaatgtagc
    22801 acttgccttc tcgcggtacc cgaacccgaa ataaaatttt agtttggcga taaaatcggt
    22861 atccgccatc gatacttcat attttttcgt atcagattca tcggaaactt ccacattgac
    22921 gaaccaataa gctttggcat gcttaggtcg ttttaaacca caccagggcg actaatcgcc
    22981 ctggtgttca ctttttttta ttttacttga actcattgac tgttttccct ttattttaaa
    23041 cattataaag accgatgagt gcttgccgcg tcttttgttt ttatcacggt tttttgtgca
    23101 aaaaaacctg ccgatagttg gcaggtcgtc tacgtgttaa gctttggtgt ggtctttagc
    23161 tggcaattgg ttttctaaat cagttcggac aacaagcaca tcgcaagctg cgtttctaat
    23221 aacatattca gaaacagaac caataaataa acgttctaca gcattcaatc ctgtggctcc
    23281 taacataatc aaatctactt gattatcttc tgggatttct ctggcaataa tttgttttgg
    23341 tgagccatat tctacgacag aggttacatt attcaagcca gctttcttcg cattgctttc
    23401 ataatctgcc aatgtttgct ttgccatttc agtagcttgt tctgcaagca ttccatcaaa
    23461 ggatgaaacg gtttggaaag aacgtgtgtc aatgacatgt gctaatagaa gttctccgtt
    23521 atttcggata gctacattga ccgccttttt aaaagctaat tcagcttcat cagaaccatc
    23581 tacggcaacc atgatttttt tgtattgttg taacataagc attcactcct caagtcaatc
    23641 gtattgtact cttttattgt agaacacttt agggaaaaag agaattattt actgctttgg
    23701 ttcagcaatt ttttttgcaa atcggatact gaaggtggca gaaacaataa ttacgatcag
    23761 taaaaagacc aaggcaataa agcgttgatt gaaaaaagct gttaaaatac ccattacgcc
    23821 taaaacagca tatgcagctc cgtaaaattg taggaatcct tgatttttat catttttgtt
    23881 gatcaataca aaaaagatat cctgtttatt aaataaataa tagctaacga ataataatac
    23941 taaagtggcg ataatcatta aaatcctaat catgaacaaa tcctccaaaa taagcaacaa
    24001 atagaaaaag caatttgcca agagcaaatt gctttattct ttaaaagatg gtaatctccg
    24061 ttaatgccgt tgtttgtcct gtagtattga tcccgcgaat tcaagcaggt gggtcccatg
    24121 gtcgtagctt cgaactacca aatgataagg catgttacca gcagtgacac aagacgggtc
    24181 ccaagatatc aataaaaatg ttcggtcaac acacaaagag gacaattcga acatcacaga
    24241 tttcccacct ctatagtagc atatgaattt aaaaagagca atgagagagc tcgcagaaac
    24301 agaaccgttt tcttgtctag ttttttggag gatgcttttt ccactctttt atacggtaat
    24361 actgttgacc cagagcctgt tcatatttcc ctgtatcttt tggctgataa tactgagcat
    24421 tcttcagttt atctggtaaa tattgctgat tgacccaagc ctgatcaaaa tgatgaggat
    24481 actgataacc cacgccacgg ttgagtgatt tcgcaccttt gtaatggcta tctctcaaat
    24541 gatcaggaac atctccagct ttgccctctc gaatatcagc aagtgctgca tctaatgcca
    24601 tgtaggcaga attggatttt ggcgacaagc aaagatcgac gacgacgtct gctagtggga
    24661 tacgtgcctc aggcaatccc aatttttcag cagctaatac agcgttcact gtacgagccg
    24721 cagcagcagg attacctaaa cctatatctt cataaccgat aaccatcaga cgccggcaaa
    24781 tacttgccag atcacctgcc tcaactaaac gggcgagata atgaagtgca gcatccacat
    24841 cacttccacg aatcgatttt tgaaaagccg aaataacgtc ataatgagca tctccatttt
    24901 tgtcatgggt aagagctttt ctttggacac attcttcaat gatcgacagt gtaagatgaa
    24961 tccttccttc tttatctgaa ggagtcgata gtgtagccag ttccagccca ttcaatgcac
    25021 tccgcaaatc gccgttcgtt gcacgcgaaa ggtgaagaag agcatcttcg tctaactgga
    25081 ttgcttgttg tccaaggccg cgttctttat cccttaaagc atgttcgact gccaactgga
    25141 tgtcttgttc tgttaatggt ttgacttcaa agatctgtgt acggctgcgg atcgctggat
    25201 tgatggtaat ataaggattc tcagtagtcg ctccgatcaa gatgattcgg ccactttcta
    25261 aatgaggaag cagaaaatcc tgttttgttt tatctaatcg gtgtacttca tccaataaga
    25321 ggatcactgt accactcatt tttgcttctt ccgctacaac ttgtagatcc ttttttgaat
    25381 ctgtagcggc gtttagcatg cgaaatgcat aattagtaga gccggcgatc gcacttgcga
    25441 tactcgtttt tccagtacca ggaggtccat atagaatcat ggaagagagc atgcgagcat
    25501 cgaccatgcg gcgtatgatt tttccttccc cgactagatg ctgctgacca acgacttcat
    25561 cgatcgttcg aggtctcatg cgataagcca atggctgttg cataggattt tcctccttct
    25621 tgtttttacg tcccttaatt atatcatatt tagcaagatg cctagaaaga acagccttct
    25681 gttgttttgt tttcatagct tatccaacta atattgtgta tgataaagta ggtgagaaaa
    25741 atgacggata aacaaaagat agatgaattt ttttggaata aaacgacgcc agaagtggca
    25801 cgggatttat tggggatgta tttggagcat gacacaccag aaggaagatt agctggatat
    25861 attgtagacg cggaagcata cctaggtcca gaagacgagg ccgcccatag ctatggttta
    25921 agaagaacac cgcgtgtgcg ggcaatgtat gaaaaaccag gtacgatcta cctttacacg
    25981 atgcatactc atcggatttt gaacatcatt actcagccag aagggattcc tcaaggagta
    26041 atgatacgtg caatcgaacc cgccgcaatg attgatcaaa tgagtaaaaa tcgtggcgga
    26101 aaaactggtc cagatatcag caacggtccg ggaaaattag tggaagcatt agcaattcct
    26161 caagaacttt atggtcagtc tatagcagat agttcgttac gcttagtttt tgagaaaaaa
    26221 aagacaccaa aaaagatcaa tgctttgcct agaatcggta tcccaaacaa aggtgtatgg
    26281 acagaaaagc cgttgcgttt tgttgtgtcg ggaaatcctt atatctcttt acaaagaaaa
    26341 aaccaaatcg agaaagattg gggttggagg aaagaaaatg aaaaagaagg atctattaac
    26401 atatttagat gaaataattg aaaaacaagt gacagactat gatgtagctt tagattggga
    26461 tacaaaaaat catacgatcg agatcgttgt tcgcttgttt gcagaaaata aagcacaatt
    26521 tgagattgat gatgcagaag ggatcgtttc gcaagaacca attatcgaat ttgaagacgg
    26581 cgtacttctt tttaatcctc agaaatctgt ttttgatgaa cgcaattatc tagcagtaat
    26641 cccttatgaa ggaaaaaaag ggttggcaaa atcagtagct gattctttag tggaatatct
    26701 aaatgaagta ttagctcaag ggcaaagcga tttgcttgat tttttggatg aagacgatga
    26761 cgaagctgta ttcgagcttc actgggataa tcagcaacta gcagaattag tagaagtaaa
    26821 acagcaaaat gaagcagata cttatttacc gtacccaagt tattaaggag tgacagatat
    26881 gaaatggaat gaagtaaaag tagaaactga aagtgaagca gtcgaagcag ttgctaatat
    26941 tttgatggag gcaggggcga gtggtgtcgc gattgaagat tctttagatg ttgaacattt
    27001 tcagtcggat caatatggtg agattttaga taaaaatgat tttacatcgc tgaaagaagg
    27061 cgcactagtc atggcttatt ttccggaaac gactttctta ccggaaatct tgccttttat
    27121 caaagatcgt atcacacaat tacctgagtt tggtctagct attggaaaaa ataaagtaac
    27181 agttagtgaa gtagaggaaa gcaactgggc aacagcctgg aagaaatact atcatccggt
    27241 gcgtattacg cgaatgctga cggttgcgcc aagctgggaa aactaccaga cagaagatcc
    27301 tctagaaaaa attatcacac tagatcctgg tatggcattt ggcacaggca ctcatcccac
    27361 gacttccttg acattgacag cactagaaac agtcttgcgt ggtggtgaaa cattgctaga
    27421 tgtgggaact gggtcgggcg tgctaagtat tgcagcaaaa tatcttggtg caaaagatgt
    27481 ctatgccttt gatttagacg aagtagccgt tcgttcggcg aaagaaaata tggatatgaa
    27541 tgaaatagcc aaagatgtcc atgtatcagc taacgatctt ttgacaggaa tcgagattga
    27601 aagtgatgta atcgttgcga acattttagc agatatcatc ttattgatga tcccagatgc
    27661 ttggcgttta ctgaagcaaa caggcacgct gatcgtttca ggaatcattg aagagaagaa
    27721 gcaaatggtt ctggatgcca tgttagaaca agggtttatc gtggatcaaa ttttccagca
    27781 aaaagactgg tatgcaatta ttttgaaaaa accagaggtg gaatagatgc agcggtattt
    27841 tttaaaagaa tcatatgaat caaaagacta ttttgtttta gaaggggaaa actatcacca
    27901 cattgtacgg gttatgagga tggaacccga ggatgaagtt tttttggcat tcaatgatca
    27961 aatagccatt gttgcagaaa tcacggagat tactgaagag gctgtttatc tgaaagaaat
    28021 taaaaaagaa gaaaatcaaa aggaactgcc aagttcgatt accatagcga gtggctatcc
    28081 taaaggggaa aagctggatt ggatcgttca gaaatccact gaattaggag cgcaagcttt
    28141 tcttggtttc ccggcatcag cctctgtcgt taaatgggat gcaaaaaaac gtatgaaacg
    28201 acaacaacgg cttgaaaaga ttgctcaaga ggcagcggaa caatcccatc ggcagatcgt
    28261 gccatcagta gagttattgg aaacgaaaca agatttattt gagcggctag atgattatga
    28321 cgtggtcttg gttgcttatg aggaatcagc aaagcaaggc gaaaaatcaa atgcagccaa
    28381 aattcttagt gaaacaaaac cagatagtcg aatcttagct gtctttggac cagaaggtgg
    28441 attatcccgc gatgaagtcg attattttgt agaacacaaa gggatacttt gtggattagg
    28501 accaagaatc cttcgtacag aaacagcacc tctttattta ttaagtgctg ctagctatca
    28561 atgggaatta atgaatcctt ctttcagcta acgttcttca ttaataaaag agtatcgaca
    28621 gaaagaattg aaaaaatagt caacaatttg tataatagaa acaattattt atacaatgga
    28681 tacgaaaaat cgtttgaaaa agcttttaaa aagcgaacat ttccgtaaaa aaacgtataa
    28741 ctgcacggat tttgggacaa ttatcaaaca gatatttgtc ccgattcccg ttttaccgaa
    28801 aaaagaaagg gggatcgatt cctgtgacaa aagaagaaat tttaacggga cccggtgtca
    28861 taaagctcgt tagtcaatac atgggtcccg aacatgtagc atttgttgag aaagcatgcg
    28921 aatacgcgac tgctgcacat gacggacagt ttagaaaatc aggcgaaccc tacattattc
    28981 atcctatcca agtcgcaggt atattagcag atttaaaaat ggatccccat acagtggcta
    29041 caggcttctt acatgatgtt gttgaagata cagaaatcac tttagaagat ctgagagaag
    29101 aatttggcga tgacgttgct atgttagtgg acggcgtaac caaattaggg aaaataaaat
    29161 ataaatccca cgaagagcag ctagcagaaa accaccgaaa gatgctgcta gcaatggctc
    29221 aagatttacg agttatcatg gtcaaattag ctgatagact acataacatg cgtacgttga
    29281 agcacctgcg agaagataag caaagaagga tcgctcagga aactttagaa atctatgcgc
    29341 ctcttgcaca tcgtctaggg atcagccgga tcaaatggga attagaagat acagcacttc
    29401 gttatctaaa tccaaaacag tattaccgta tcgtccactt gatgcagacg aagagagaag
    29461 aacgtgaaaa atacgtaagt ggtactgttg aagatattcg aatagcgaca gaagagctgg
    29521 ggatttttgc agaaatctat ggacggccaa aacacattta ttcaatctat cgtaaaatga
    29581 aagatcagaa aaagcagttc aacgaaattt atgacttgct agctattcga gtgatcgtag
    29641 attcaatcaa ggattgttat gctgtattag gagcaatcca cacaaaatgg aaaccaatgc
    29701 ccggcagatt taaggattat attgccatgc caaaagctaa tatgtaccaa tctttgcata
    29761 ctaccgtgat cggtccggca ggaaatccgg tagaaattca aatccgaaca caggaaatgc
    29821 atgaaatcgc tgaattcggg gttgctgcac actgggccta taaagaagga aaaaatgaaa
    29881 aagtagaacc agatggtatg acgaaacaat taagctggtt ccatgagata ctcgaacttc
    29941 aagacgaaag ctatgatgct tctgaattta tggaaggcgt aaaaggagat atctttagtg
    30001 ataaagtcta cgtcttcaca ccaaaaggag acgttactga gttaccaaaa ggatccggac
    30061 cattggactt tgcatacagt atccatacag atatcggtaa caaaaccact ggtgcaaaag
    30121 taaatggcaa aatggtgcag cttgattaca aattgaaaaa cggagatatc attgagatca
    30181 tgacttctcc aaattcattt ggcccaagtc gcgactggtt gaaattagtt gctactagca
    30241 aagcaagaaa taagatcaaa cgtttcttca aagcccaaga tcgagaagaa aatgtgatca
    30301 aaggccacga atccgtggtc aaatgtatta cagatctagg atttacgcct aaagatattt
    30361 tgacgaagaa caaactgcaa gaagcactcg atcgttttaa ttatcaaaca gaagatgatc
    30421 tctatgcagc tgtagggtat ggagaagtta gccccttgac gatggccaat cgtctgactg
    30481 aaaaagaacg taaagaacaa aaaatcgagc agcaaaagca agaagcagaa gaaatcatga
    30541 atcagccgaa aaaagaacct gacaaaatga aagtacgtca tgaaggtggc gttgtcattc
    30601 aaggggtaga aaacttacta attcgtatca gtcgctgctg taatccgatt cctggtgatg
    30661 atatcgttgg ttatatcact aaaggcagag ggatatccat tcatcgtcga gattgtccga
    30721 atgttcagcc tgacaaacca aatgtagcag aacgtttgat tgaagtcgaa tgggaagata
    30781 catcgaatac acgaaaagag tatgatgcag atttggaaat ttacggctat aatcgttcag
    30841 gcttattgaa tgatgtactt caaacagtca atgcgctaac gaaaaatctc aacagcgttg
    30901 aagcacggac gaataaagat aaaatggcga cgatccattt gacggttggt atccagaatt
    30961 tatcccacct aaagagtatc gtggataaaa tcaaagcagt acctgatgtc tacagtgtac
    31021 gccggacgaa tggataggag gacagatatg agagcagttg ttcaacgggt aagtaaagct
    31081 gctgtgtcag tcgatcagca agaaattgga aaaattggct acggcctact tgttttatta
    31141 ggcattcatg aaaccgatac gcaaaaagat gtggactatc tggtaaaaaa aattgcacaa
    31201 atgcgtattt ttgaagatga acaaggaaaa atgaatatta gtgtagaaga tgtgggagga
    31261 caaattttaa gtatctctca attcacactg tttgcggata cgaaaaaagg aaatcgacca
    31321 agctttgtgg aagccgcacg accagaaaca gctattcctt tatacgaatc attcaatgaa
    31381 ggaatcagaa gtcgcggcat cacagtcgct accggacaat tcggagcaga tatgtcggtt
    31441 tctctcgtca atgatggacc tgtaacgatc attattgatt ctaaaaacaa ataaagtaat
    31501 ctggttttca ctagtaaaaa ctatgaaaca cgtcgcttaa gttagactga taaactctaa
    31561 cttaagtggc ttttttgtct gcaaaaagcg cttcttttgt cagcagtcgg aaaaaaagtt
    31621 aagcttaaat tatacaagtt attttttata ggaggttgct gaagatgaaa gaagtaccag
    31681 tagtagatat caaaatcaca gatttaaaaa aattgtatca aaaaggatac aacatgttgg
    31741 aggagttacg ccatgaagcg gacgctcccg tcgtcaaagc aaagattttc aataaagaag
    31801 ctatcacgat ctacggatca tcagcagcaa aagtatttta tgatccaagg aatttcaaac
    31861 gaaaaggtgc tatgccaaaa ttagtattga agacactttt tggtcaaggt ggagtacaga
    31921 ctttggatgg agctgctcat caccatcgga aaaatatttt tatggatcta atgacaccgg
    31981 aaagaatgga agattatcat cgtatcttag ataagaatct gacacaagca cttgaagcac
    32041 agcatggtca atttgaacta tttgatttaa gtaagatggt tttctttaca tctatttgcg
    32101 agtgggcagg tatcaattta tcagcgattt ctaaagatga ggtcgaaaaa ttagcagaat
    32161 atcaaatctc tatgatcagc ggaacgttta cttctccgat tgatcacata aaaggtgtcg
    32221 aaaatcgcaa aaaatcagaa aaatgggcgc aaggcttgat tgaagaagct cgacaaaatc
    32281 ctgtggctgg aaaagaaaat gtggcattat atgcttttgc caatgccaca gatttagatg
    32341 gtcagctttt acctctggaa gtggcagctg ttgaattatt gaacatcatc cgtccaacgg
    32401 ttgctctaac tgtgtgggcg gcattgatgg gacatgcact tttcagtaga ccagatttgt
    32461 atcagcaatt aaaaaatgac ttttcgacgt tacaagatcc gtttattcaa gaaatgagac
    32521 gctattatcc tttcttccca atgctacctg cgatttcgct gaaagaagta gaagtagatg
    32581 gttatcgtat accagaaggc agttgggtta ttttagattt atatggaaca gaccatgatg
    32641 aacgaacagt tgaagcgcca gattccttta tgatcaaacg gtatgtaggg aaagcaaaag
    32701 atatttccta taaagaagaa tatgagatga ttgcacaagg cgggggaaat tttcgacaaa
    32761 tgcatcgctg tgcaggagaa tggatcactc ttcatagtct acgtgtgttt tccgatcagc
    32821 tagtaaataa gtttgagttc tctgtacctg aacaagattg gacgattcca ttcaatcagt
    32881 ttccgactta tccgaatagc cgagcgctac tttacaagaa ttagattagc ataaacttat
    32941 cgaaaaaaag gaagatcaaa cagtgctgat cttccttttt tcatattact ataaattgat
    33001 aaggtcaatt gatcaaaatc cacgaaatgc ttcatcaatg gtttgataat cttcattgga
    33061 taaatgcaaa tctaaagctt ggacattgtc agatacttgc tctggacgct tagctccagg
    33121 tataactaca gacactcgtg gattcttcat gtaccaagct aaaacaagtt gcgcaggagt
    33181 ggcttggtat cttttagcaa tggggcgaag tacatcgaca gcggttaaaa tttctctgaa
    33241 acgttctcct tggaaatctg gatttctttt tcgaggatct ccttcaccaa attgtttttc
    33301 ctcaccaagt tcatattttc ctgtcagcaa accggaagca agaggaaaat agggaacaaa
    33361 actgattttg tttttttcaa gataagggaa taattctttt tcagcctgcc gatggatcaa
    33421 actgtattta tcttctacta catcgacata tccatctgca ttcgcttctt tcaattgttc
    33481 taacgtgaag tttgacacac cgactgctcg gatttttcct gcttctttta attcgtgcaa
    33541 tgtagcaact gattcatttt ttggtgtgct ttcatccgga aagtggatat gaaaaatgtc
    33601 gatgtaatca gtttgcagac gttttagtgc atcttccacc gattgtttga gaaactcagg
    33661 agaattatca aatgttcgcc ctttatttgg tacatgagca gcttttgtag cgatgacgac
    33721 acgtgagcga tcatattctt tttccttcag tacctcgcca atcagttctt cggaacggcc
    33781 gtttccgtaa gcataagcgg tatcgatcaa attgatcccg ctattcaaag ctgtacgaac
    33841 tacttgtttt cctgtctcat cttccaaacc gtcaaaaaga ttatgtcctc caacggcatt
    33901 tgctcctaat ccaagctgtt ctgcgtagac ttgtgaatgt ccgattctta tttctgccat
    33961 agctgattcc tcctttttgt ctagtctaaa acaaaatacc aaaaagctga actaaaacgt
    34021 ttttgtgagg ggaaatagtc agatatctct tgactttgag actggttttt agtaggttat
    34081 aagtaatatc gaatgtcaat gaaagagcga gtaagttttt gaaactttac aagagaggat
    34141 catcgaggct gaaaatgatc taaagtttca ttagcctaaa gacactctgg agatggacga
    34201 gtgaaaagcc gtcccgcgta cgagcgttat tcgtaagagg aaagagaaat ctttcaaaat
    34261 taggtggtac cgtgtatgaa tttcattcac ccttgttcat gatgaacaag ggtgattttt
    34321 ttatttacat agctaagaaa gggagagaaa actatgagtt tccaaagacc aaaaggaaca
    34381 aatgatattt tgccaggcga atcagaaaaa tggcagtttg tcgaagaaac agcacgttta
    34441 ttattcagag attatcagta taatgaaatg cgtacaccga tttttgaaca ctatgaagtg
    34501 atatcccgaa gtgtagggga tacaacggat atcgtttcaa aagagatgta tgatttttat
    34561 gacaaagggg aacgccatgt gacgcttcga ccagaaggta cagcaccaat cgttcgttct
    34621 tttgttgagc ataaattgtt cggcccagag tatgcgaaac cgttcaaaac tttttatatg
    34681 ggaccaatgt ttcgctacga acgccctcaa aaaggacggt tacgtcaatt ccatcaaatc
    34741 ggagtagaag catttggatc agaaaaccct gcaactgatg tggagagcat ggtgatggca
    34801 cttgatttct tcaaacagtt ggggatcacg caaatccggc tagtcatcaa ttcattaggt
    34861 gataaagaaa cgcgtcaagc ctatcgtcaa gcattgatcg actatctttt accttttgaa
    34921 tcagagctga gtgaagactc taaacgccgc cttcatgaaa atccattacg cgtattggac
    34981 agtaaagaca aacgagatca aaaatttgtg gcagaagctc catctatctt agattatcta
    35041 agcgagcctg ctcaaaaaca ttttgatacc gttgtttcca tgctggatgc attggctgtt
    35101 ccttatgaaa tcgacagtaa tatggttcgc ggactagatt attacacaca tacgattttt
    35161 gaaatcatga gtgacgcacc aaaaatgggg gcacaagcaa cgatttgcgc aggcggacgt
    35221 tatgacaatc tagtggaaga actaggtggt ccttcaacac ctggcttcgg atttgcaatg
    35281 ggtatcgagc gattactctt gactatggag gcagaggaag tagttatccc tgcatttaat
    35341 gagttggatg cttacgtggt cgcactcgga gacgaaacaa atatcgaagc attgaaagtg
    35401 gtccaagcga ttcgaaactt tggtttttca gctgaccgcg acttcatgaa ccgtaaagca
    35461 aaagcgcaat ttaagacagc agataaagcg aatgccaaat tagtattggt gatcggtgaa
    35521 gatgagcttg ctagtcaaac gattaatgtc aaagcaatgg caagccgaaa agaaaaaagt
    35581 tttccattag aagagatata tgaacatttt gatgaagtct atgatgaaat gactttattc
    35641 gagtaggagg aaaaaaataa atggcacaaa gaacgatcta ttgcggatta gtttccgaac
    35701 aacaattaga acaaacagtt acattaaaag gatgggtaca aaaaagacga gaccttgggg
    35761 gagtaatctt tatcgaccta cgtgatcgtg aaggaatcgt tcaaatcgtc tttaatcctg
    35821 aaatcaacaa agaagcatgg gaaatcgcgg ataaatgtcg cagtgaatac gtgatcgaag
    35881 tgactgggga agttagaaga cgcggagaaa atgcagtaaa tcctaaaatg gccactggtg
    35941 aattcgaagt gattgcaagt gaaatcgtga ttttgaatca agcaaaaacc ccgccatttc
    36001 aaattgaaga tgatcaagca atcagtgacg aaatacgttt gaaatatcgt tatttggatc
    36061 tgcgtcgtcc aaaaatgact aaaaacatca aattaagaaa tgcgacaaca aaagcgattc
    36121 gtcattattt agatgataat gactttctcg atatcgaaac accttaccta gggaaatcta
    36181 caccagaagg cgcaagagac tacctagttc cttcacgtgt ccatgctgga catttctatg
    36241 cgttgccgca atctcctcaa ttgtttaaac aattgctgat gaatgctgga ttcgaccgtt
    36301 attatcaaat cgttcgttgt ttccgcgatg aagatttacg tggagaccgt cagcctgaat
    36361 ttacgcaagt agatattgaa acaacgttct taacagcaga agaaatccaa acttacacag
    36421 agggattgat tgcgaaggta atgaaagaag ttcgcggtat cgaagtcaca ttgccatttc
    36481 ctcgtatgac ctatgatgaa gcaatggctc gctacggcag cgacaaaccg gatacacgtt
    36541 ttgatatgga attgatcgat ctaagcgata cagtcaaaga agtagaattc aaagtcttcc
    36601 aaatggctct tgaaaacggc ggtgtcgtga aagcattgaa tgctaaaggc gctgcagatc
    36661 gctactcacg aaaagacatg gatcagttag gtcaatatgt aggacaattc ggagctaagg
    36721 ggcttgcatg gctgaaagta gaagaagacg gattgaaagg tccaattgct aaattcatgg
    36781 gagaagcaac agaagcaatc attaaagcaa cagatgcaaa accaggtgac ttgctgatgt
    36841 ttggcgcaga taaaagtgaa atcgtcgctg ctgcattagg tgctatccgg acacgtttag
    36901 gaaaagaatt aggactaatt gatgaatcga aattcaattt cttatgggta actgattggc
    36961 cacaatttga attttcagaa gaagaaggtc gatatgtctc tgctcaccat ccattcacga
    37021 tgccaaaaga agaagatatc ccgttattgg ctgaagaccc tgcaaaagtc tatgcagaag
    37081 cttatgatat cgtcttgaac ggttatgaac taggcggagg ttctatccgt attcatactc
    37141 gtgagcttca agaaaaagta ctagaaactc taggctttac aaaagaacaa atggaagaac
    37201 aatttggttt cttgttaaca gctttagatt acggattccc tccacatggc ggtattgcat
    37261 taggattgga ccgttttgca atgttattag ccggagaaga taatattcgt gaagtcattg
    37321 cctttcctaa aaacggaaaa gcagcagatg tgatgagtga tgcaccaagt atggtttcgc
    37381 ctttacaatt gttcgaattg aatattgatg taacgaatat agaagaatag ccgataaaaa
    37441 ggtaaaaagg atttgtcgaa tacgcttctt ttgacaacct tgatttaatt gaaaaacact
    37501 ttataatgaa gccataacat ataaagcggg gcttttccat gaaaaaaaca ttcgaaaatt
    37561 gggcttatca tgattacacg accaagcttt cggtatctat cgtttatgcg ttgatcgcat
    37621 cagtcgcatt gaatttcttt tatcagccag gacatattta ttcaagtggt attactggtc
    37681 tagctcagat tttgacgact ttgtcaaaat cactgatagg attcgaagta ccggtatcaa
    37741 caactttgta tttactgaat atacctttgt ttgtattggc gtggttaaaa atcgggaaga
    37801 agtttacaat tttcactttt atcacagtca ctttgacctc cctttgtatc catattttac
    37861 cggaaacagt cctatctacc gatccaatca tctgtgcgtt gtttgggggg gctgtgatgg
    37921 gactaggtat tgggctagcg ttgagaaacg gcctatcatc gggcggtcta gatatcgtga
    37981 gtatcacgat tcgtaaaaaa acaggaaagt cgattggttc tatttcgatc tacttcaatg
    38041 cattgatcgt ttttgttgcc ggaattctat ttggctggca atatatgttt tacagtacgt
    38101 tgtctatttt cgtcagtgga aaagtaactg atgtcgttta tactaaacaa aagaagatgc
    38161 aggtaatgat cgtaacgaaa catccagaaa aagtagtaaa tgaaatccaa aactgtatgc
    38221 gtagagggat cacgatcatc aatgaagcag aaggtgctta taagcatgat aagcagacag
    38281 tattgatgac gatcgttaca cgatacgaac tgcctctact tagagaagca atgaaagctt
    38341 ctgatccaca agcttttgta agtattgcag aaaacgtcca gatacttggt cgtttctatg
    38401 aggatggcat ttagatcgag tttgacaaaa attcatgaaa tactgctgta gacagacgtt
    38461 cgtttgtctg cagttttttt ctaccatagt agaatcagca aagaaaaact gcttttttat
    38521 ttgaaatttt cttaaagttg aaaaaaagca tgtttatttt tttaaattta tgggatgata
    38581 gaaaaaaata gtagagaaag gacgcgattc tacttgctat tttcacttaa tacccgtata
    38641 ttacgaatga aaaaagttta tgaaaagttg gaatattgaa tggaaaaaca aaaaaggtta
    38701 aaaaacagcc gctacatcac tgggtttgat gggctccgtt cattagctgt ggtgggtgtt
    38761 atcttctatc accttctgcc aactagtatg aaaggcggct atttgggcgt tccgattttt
    38821 ttcgtcgtat ctggttattt gatcacggat ctgctgcggc aggaatggga acaaaatgga
    38881 aaaatcaata tttggcaatt ttatgtcaga agaatgaaga gactctatcc ggcacttgtt
    38941 tttatgcttg tgacagcttc ggcttatatc actttgtttc agcgagggct gttaaacaat
    39001 cttcgaggag ttgtggtcag cagcttactg tatgtcaaca actggtggca aatcaataac
    39061 ggtttatctt atttcgatcg atttgctaat gagtcaccgt ttacgcatat ctggtcttta
    39121 gcagtagaag gtcaaaacta tttggtttgg ccgatcttgt ttgtattgtt gatggtattt
    39181 gttcgtaaaa gaaaatggat cttttatact gtgttaggta catccttcct ctccgccatc
    39241 ttgatgatga tcttgtatac accaggaggc gatccaaccc gtgtttatta cggtaccgac
    39301 actcgtctgt tttctatatg gttgggaagt gcgcttgcgt ttgtttggcc aagtacgcgt
    39361 ttaaagaaaa acatcccgaa acaagcgaaa aatattctga atatcgctgg aggaatctcg
    39421 cttttggcac tgattctttg cttcttcttt ttagatgatc attacaattt catctattac
    39481 ggaggaatgc tcctagtgag tctgttatgc gtagtgctag ttgcagtgac agcacacccc
    39541 ggagcaagtt tgaatcgatg gctgacaaat ccagtattta cttggattgg caaacgaagc
    39601 tatggcattt atctgtatca gtttccagtt atgatttttt atgaagcaaa ggtgactgat
    39661 attgccgatc atgttttgtt gcatacttta gtggaaatca cattgattct aatcatcagt
    39721 gaactttctt atcgatatgt ggagcgtccg ctagcacgtt ttgattatgg tcgtacattc
    39781 caagtgatga aaggatggtt cagcaaaccg atcatcagta agagcaaacc atgggttgtt
    39841 ccaggcacat tacttgtagc aattgcactc gttggtttcg ttacggcacc gaaaaacgct
    39901 gtaacagccg atcaaaaaca actgcaggca aaaatcgctg aaagtaaaaa attagcagaa
    39961 gaaaccagaa acaattcaga aaacaccact agttcggttg accaagcgat ccttgataag
    40021 tacgagttgt ccaaggcaca agggaataag gcacaaaaat tagaactaac ggcatttggt
    40081 gattcagtta tgttggatgc tgcaacggat ctacaagaac tttatccaaa agtagtggta
    40141 gatggagatg tcggacgaca attatatgcc agtgtaccgt acattgaaaa attaaaggaa
    40201 caagatttgt tgaaagacac tgttctgatc ggactaggaa cgaatggctc attctcggaa
    40261 gctcagtttg atagtgtgat ggatgcaatc ggtgatcgaa aagtatactg ggtcaatgtc
    40321 cgagtaccga cgaagcgctg gcaaaatgac gtgaatgcga tgcttgaaca gatgacgaaa
    40381 aaatacgaca atatgacgct gattgattgg tacagctaca gtaatgacca tagtgactgg
    40441 ttttatgatg atcaagtcca tccaaatcct gaagggatga cacattacat tcacttagtc
    40501 agcgaagcct tgctaggagg aagttcaagt acgaacagca gtgagacaaa cgtgacagaa
    40561 tgatagctac aaaaaacttc aggcaaaaac atttgttttt gcctgaagtt tttttatatt
    40621 taccgtgttt ttaacattca tatagattca ggaagcaaaa cggttgagtg tgtctgtaaa
    40681 taacgttctt tttgttttcg ggtgagtttt ttgaatgcag cttctgcttt tgttgcttcg
    40741 cttcttgaag aaaatacttc tgcatggatc atcttaacag gtcttctttt ttgaagacga
    40801 gtatatttag caccagtacc attgttatgt tcctctaatc gtctagaaag atcggttgta
    40861 tatccaccat aaaatgtctg atctttacag aataatacgt aaaaataatg ttcatttgcc
    40921 atagagcatc tcacgaatct ctgcagtgta ttctccatcc atattgtaag tgatcaatgg
    40981 tggagcgact ttgaatccgt ctaatttgcc ttctttgatt ccttcaacta acaaaatatt
    41041 tgcctctttt ccaggttttg ggtaaacaaa ttggatcctt ttaggtgcaa ttcgatagtc
    41101 ttccatgacc tgaatgattt ctaagaaccg atcaggacga tggaccatag taaagcgtcc
    41161 atttgttttt agtagcttac ttgaaatatc gatcacttct tctaacgtcg tatgaatctc
    41221 atgacgggcg attgccaggt gtttattagg gttcgtttta tttgttggca gccctttgaa
    41281 ataaggtgga ttacagacga ctaagtcaca agaattatgt cgaacgacag ataagctgtc
    41341 tttcagatcg atggtgtgga cagtcatttg ttcatttaaa tggttaagcg cgatactacg
    41401 tcttgccatg tctgctagac gaggttgaag ttcgatagcg tcgatagccg cctgggttcg
    41461 tttacttaaa aaaagtccaa ctgcgccatt tcctgcacaa aggtcaacaa tcttcccttt
    41521 tttaggtaca cgggaaaagt gagcaagcag cacagcatct aaagaaaaag aaaacacttt
    41581 ggaactttgg ataatcttga tatcttctgc gtataattgg tcgattcgtt cgtcttcgtg
    41641 aagcatggat tccctccaaa atgaaaaaaa tagtcgtctg tattatacta tttttattaa
    41701 aaagaaccaa ggtggcttgt caaacctcta ttttttatgc atactatgac cataagaagg
    41761 gaaaggaaga gactatgttt tatcgattta tgcgcggggt agttcgagta ctattagcct
    41821 tagttaatgg aaatgcccgc tatgagaata aagaagtttt accgcaagat gagaattaca
    41881 tactagttgc tccccatcgt acttggtggg acccattata tctggcggta gctgcagcac
    41941 ccaaggaatt cagctttatg gcaaaagaag aactgttcaa aaatccagtt ctgcgattta
    42001 tattaactcg atccaatgct tttccagtta aaagagaaaa tccaggacca agcgtcatta
    42061 aaacacctgt aaagattttg aaatcgacaa atttgagttt gatcatgttt cccagtggga
    42121 caagacatgc tacagaactt aaaggcggaa tggcattgat ttccagaatg tctaaagcga
    42181 aaatcgtgcc agctgtatat caaggtccgt tgacacttgg ggatctgttc aagcgtcaga
    42241 gagtgacagt gcgtttcggc gaaccaatcg acttatcaga tatcaagaaa acggataaag
    42301 aaggtatggc ggagatcgaa cgacgaacac aagcagcttt tgacaaattg gacaaagaag
    42361 tgaaccctga tttcaaatat gaagtcaaaa aaaaataaaa tgataagttt atgcgtgggc
    42421 ggtcttttga atctactgtc ctgttcagtt cagacttttt caatgaaaac gagggagaga
    42481 cgtcaagtct gccctctttt tgcgttccaa tatgtaaaat agaagacagg tgtcatgtaa
    42541 tggatcaaag tgttataata attagaagga cattttttga ggtgattgaa tgcgtttttt
    42601 acatacagcc gattggcata tcggaaaaaa actacaaggt tatgacttga ttgaagacca
    42661 gcgtcatgta ctatcttcta tactagatat cgcgaaagaa gaaaaagtag aggcaatcgt
    42721 gattgccgga gacttatatg ataggagtgt cccagctgtt gatgccgtag aattatacaa
    42781 tgaattgatg gtcgattgga atttaaagga aaagatcccg atttttgcta tatcaggaaa
    42841 tcacgatagc agtacgagac tttcagcggg tacgccatgg ttcagttatt cgaactattt
    42901 tttgcgaaca cagctagcag aagcatttga accagtcgag tttaaaaacg tacaattttt
    42961 ctttcttcct tactttgaac caattgcagc gagattatat tttgaagatg acaatatccg
    43021 aacgatcaag caagcggtag ccaaggtgat agataaaatg caggagctat ttgattcaga
    43081 aaaaaaacaa gtattgatta gccatttttt tgtaatggga agtgtaaaat ctgattcgga
    43141 aacgaaaatc gaagtaggcg ggttggattc gatccctggt gaactactta caccgtttga
    43201 ttatgtagca ctaggacatt tgcataatca agctgctttg agacaggata atgctcgcta
    43261 tagcggttcc ccattgaaat tctctttatc tgagatcaac caaaaaaaag gcgtatggat
    43321 cgttgacctg acaacaggcc tggagttgaa ttttagggag atcgaacccc tgcatgatat
    43381 tgaagaaata acagcgagtt tcaaagaatt gctggatcct gccttttatc agtccgtcaa
    43441 tcgggagaat tacatctata tctatctcac tgatagaacg atcatcccga atatgatgaa
    43501 ccagcttcgg caaatatatc ctcgtatttt ggggattgaa cgggcaaacg gtcgagcaga
    43561 acaaacaaag cgcaaacgaa aagtactaga aatgaaagat cctcaaaaat tagcagaagc
    43621 attttttagt gaaatgacgg aagagcagct aactgcaaac caagaaaaat ggctcggaga
    43681 aacgttgcaa gaaatcatgg aaatggaatg atcaagaatg aaaccgaaaa aattacaact
    43741 taaaaatttt ggtcctttta tcaatgagac cgtagatttt tcacgtttga cagaagcacc
    43801 gctttttttg atcagcggga agacaggagc tggcaaaacg acgatttttg atgggatcac
    43861 cttcgcactt tttggtgaaa cttccggaag acttcgctct ggaaaagaga tgcgttcttt
    43921 gtttgctaca ccagacgaag aaacaagcgt gactttttct tttgaacatc agcagatgag
    43981 ctatgaaatc gaacgaaaac cagaacaagt tttagcaaaa cgaaaaggaa acggaacaaa
    44041 aaaacaggca gccaaagtaa cgttgaccat ttttgatggg aaaggaaaag aaataaaaca
    44101 aatcacaaaa cgtacagaag cagatcagtt gatcagagaa ctgatgaatc tcgatgcgaa
    44161 acagttttct caaatcgttt tactacccca aggagaattc cgtaattttt tggtttcatc
    44221 aagcagtgat aaagagactg ttttacgtca tttattcggt actcaatttt tccaacagtt
    44281 caatgagcga ttaaaagaaa aagcaaaaca acagcaacaa aaattggatc atttagagca
    44341 agagctgcat cttctgcaaa aaaggtttgt tttagtcaaa gaagaggaac cagcagcagg
    44401 gtttgaagcc gttttagccc aatggacaaa ccatcaggca atactcgaaa aacaaatcac
    44461 ggaagaaaaa gaagctctaa cggaacttca ggaaaaacaa aagcaactag aaaattccta
    44521 ctatcgtttt gaaagcttga aaaaaagtta tgaagaaaaa acgatattaa taaacaagca
    44581 aaaggaatta gctgctcaaa aagacgaaat agaggaaaag aaacgatgga ttgggtatta
    44641 tgaatttacg caacgattag tcgaaccaat ccagcgccta caagaaataa aagttgaaaa
    44701 aatagaactg tcacaaaaaa gtagtgagca aacaaagaaa ctagcgatga ctacgcagaa
    44761 ttatgaagag tggcaaaaaa atgaagcaaa ccgcgtacgc cgacaaacaa aaatcaaaga
    44821 agctgctcaa aaactgcaaa acaaacagat gatggtgcca attgtcgaat ccttacgtag
    44881 taaaaaacaa gacgcagaac aattaacaat ttcattggat aaagaagaat tgaatgccga
    44941 gaagctgtat caaaatctcg ctatctatca ggaaagaact cgaattcttc aagaaaattt
    45001 actggatcag gaaaaactgc aagaagatcg tcttgctttt ggacagttaa aatatctaga
    45061 tgaaaagcag caagaaatag aaaaacaaaa agcaaaagac actgggtcgc tcgcacagat
    45121 aagagaagaa ctcaaaaaag caactgaaga aataacaaaa aatagtcagc tattgaaaga
    45181 acagacagaa caagcagaac agctgaaaag ccaatgggca aagcagcaaa ttgctcgtct
    45241 tcagcttttt ttgcgtccag gggagccatg tcttgtttgt ggatcaacgg aacaccccat
    45301 acaacaatta aaacacgaac agccaaataa ggaagatatc ttaaaagtag aacaacaact
    45361 ggcagaagct gagtctgctg tagaaagaac tcagcaaatc attgccaaaa gtgaagcaca
    45421 tgaaaataat cttcaacaac aattatcaga tttagaacaa aaaatcatgg caacgaatat
    45481 acaattggct gaacaagaag aacacttgca acaggctttg aaaaaggcat ttcctgagat
    45541 aacagaacaa aaaacaaaaa caactcttgt agtaatcgaa caatctttag atagacgaaa
    45601 aagcaagatg gccaatgcaa gaatagagct ggaagaaata agacaacaag cagaaaagct
    45661 cgaacaacaa ttagctgtct acaaacagca gcagacagaa aaaaataatc agctccagca
    45721 actgcaagga gaaatcaaaa gtttagaaca acaattgcaa gatacggata gtaactgttt
    45781 agaagaggag atacgacgac ttgaaaaaca gttagaagaa gactcgaatc aactagctgt
    45841 tgagcagaaa gcaggagtgc aattgttgca agatcttcat acgcttcgga cacagcttga
    45901 tcttcaacat gaacaaatag aaaaaataga tgaaaaaata gaaaaggaac aagaaagaat
    45961 agctaaaaag ttagaaagcc agtcgtattt ttcaacaatt gaagaaatct gtgcatttgc
    46021 acaacagcaa gcacaatata aagaaaacca acgtacagtt acttcttatc atgaagagca
    46081 attagtgaca gctgaccggc tgcgtcaatt agaaaatatc cagctaactg agacattccc
    46141 agatacagaa gagatcagcc aacagttaga agaaataaga cgtatgatcg aaataaagca
    46201 agaaaagata tacggtttac aagaacaaaa aattagtaat caaaaaatct acgatgaatg
    46261 tctttcgatt tatcacacaa gccagacgaa attagatgag cttacgcaat tacagcagct
    46321 ttcgcaaaca ttcaacggag aaaatcctaa gaagactagt ttggaacggt atgtgcttca
    46381 ggtttatctt caagaagtgt tacaagtagc aaatgatcat ctccaacgtt taacgaaaaa
    46441 tcgttatcaa tttgagctag cggataccat tggcagttat cgagggaaaa ccggattaga
    46501 aatcaatatc tatgatgatc aggcaggtat gtctcgaagt gcgcataccc tgtctggcgg
    46561 tgagagtttt attgcagcac tttctttagc attagctctt gccgatgtga tccagacaca
    46621 agctggtggt gtagcaatcg atgcattgtt tattgatgaa ggatttgggt cattagatga
    46681 agaagctttg gaaatggcaa tggaagcttt agaaacgatc gaaagtgaag gaagaatgat
    46741 cggcatcatt agtcatgtaa gagaattgaa agaacgagta ttacaacaaa taagaattga
    46801 tacagaaggt agcggacaaa gcaaaatacg ctatcaactt ggataaaaag ggagattggt
    46861 aactatgaaa tggagtattc cagaaaaagt cattgcacgt ggacgtcaat acttgcaaga
    46921 aggccgagtg ttatcggttg tccctgacca agaagaaaac gtgtggcatg cagaagtctt
    46981 gggcagtgaa ttgtatatgg ttgatttgga tggtacagca aaagagatag attactgcca
    47041 atgtccctat tgggaggaac ataaatattg taagcataca gttgctgtgg aactttattt
    47101 acggtcgaaa aatctatctc gtatcatgaa aaaagaccag ccaatgctag aaccggtaaa
    47161 agcaacaagt gagggagaaa tcattgcaaa aggtttttct cgtctaaaaa cacaaaacaa
    47221 tcctcaagcg gtgcaacctt tgattattga ataccaagta gaaacgattg agactaatca
    47281 gtatcaccca gagctttcta ttttggcact ttacttgcgt atcggttcat tggcatcacc
    47341 gaaaaaaaca tatatcgtaa aaaacatcta tgatttttta caagcttaca cagaaaaaga
    47401 aacttacact gttaataaac agtaccagtt tcgtctagat aaacgggcgt ttcaaaaaga
    47461 agatcaaaaa atactcacgc atcttgcagg aagtgctcag acgcatcaat tattaggtac
    47521 gaacggctta caagtcaaag gaaaactaga taaaagatat ctgcttctcc cagtagaaca
    47581 agcccgcttc ctgttagagc agatgaatca gactacacgt ttgcatctgc agataaatga
    47641 acaaaacgat caaggaatac aattttccga tcaaaacaaa cctttatcat tcaaagtgga
    47701 aaaatttgga gaagattatt tgctgcaagc attgaatgat tttgattttt tctttatgca
    47761 ttatcagtgg ggagttatcc aagggacatt ttattctctg acgaaatatc aacaaacggt
    47821 ttatcaaact tggaaacaat tgattcgccg attggagaaa ccagaagtag tgttcaagaa
    47881 aaaagaatta ccgtctttgt ttaaagagat tatccctctg ctgtcagaga tcggcgacgt
    47941 agctattgcc cctgcagtag aagaggaagt agcagatatc cccgtcgaat ttattttctt
    48001 tttccgaaaa gcaaaaggaa aaatccaagc tcgagtcgat ttcgtctatg gcgaagtcat
    48061 ctattcgact gacccgaagc atgaggtctc gacagaacag gcttttcagg tgttacgtga
    48121 cacagtagaa gagaaacgag tacttgatct attcagaatg tatcgctacc agaaaaatga
    48181 aactggttat gaacgtccat tgccaagcgg ggaagaacta tatgcctttt tccgcacaga
    48241 actatctgtc ttccgtcagt ttggtgaagt cagacttgga agaaaattgc gagagttgta
    48301 tttagatgcg cataaacatc aaccgaaaat cgaagtaacc gagaccggtt catggctcga
    48361 tattcgattc gatgtgacag ggatagagga gcaagaaatc gatcatgtcc tgcaaagctt
    48421 gctgagaaat gatgcttttt atacattaga aaatggtcag gttctttctt ttgattcaga
    48481 agaatttcaa caaacaagcc aagtgttgca acaattacgg gaatcgatac gtacagaaga
    48541 aggaacgatc catgtaccta aaaatcaagg attgatcatt caaaatcaat tggaaaaaag
    48601 caatgctact tttagtgaat cgttccaaac gatggtacaa gatctcattc atccagaaag
    48661 ataccaggca cagcttccca aaggattgaa tgcaacgatg agagattatc aaaagcaagg
    48721 ttttcgatgg ttgaagatgt taggtcatta tcagtttggc ggtattttag ccgatgaaat
    48781 gggacttggg aaaacactac agacaattgc ctttttactt tcggaaaaag aagaacggaa
    48841 aagcttctct gctttgatcg tagcgcctgc gagcttaatt tacaactggc aggcagaggt
    48901 gagaaaattc gccccttctt taagtatcca agtaatcaat gggaataaaa aagaacgtga
    48961 agaattactg gctaaagaca cagatatccg agtgacttct tatgccagct taagacagga
    49021 tctagctaat tatcagtcgc aaaaaattga ttatttgatt cttgatgagg cacaaatggt
    49081 caaaaatagc agtacgaaaa ctgcacaagc attgagagaa ttagctgtac ctcagcggtt
    49141 tgcactgagt gggacaccaa tcgagaataa tttagaagaa ttgtggtcat tgtttgcaac
    49201 gattatgcca ggatttttcc cgactaaaac aaaattcagg gaacttgccg cagaagaaat
    49261 tgcgcaaatg attcgaccgt ttattttacg gcgagataaa caaactgtgt tgaaagactt
    49321 gccggaaaaa acagagatga atctatattc tgcacttaca gaagaacaaa aaactgttta
    49381 tttagcttac ttgcgtcaaa tgcgtgaaga aatcacatca atggattctg aagcattcaa
    49441 gaaaaaccgg atcgggatac ttgctggtct gacaagactg cgacaaattt gctgcgatcc
    49501 tcgtctgttt attgaagatt accaaggcgg ttccggcaag ctcgaacaag taaaagatct
    49561 actggtagct gccaaagaga acaaacgaag agttttgctg ttttctcaat ttacagggat
    49621 gctgactatc ttgcaagaag agttagccga gctagggatc tcgacatttt atttaagagg
    49681 cagtacgaaa ccacaagatc ggttaagtat ggtagacgca tttaatgctg gcgaaaaaga
    49741 tgtttttctg atttccttaa aggctggggg aactggattg aatctcacgg gagcagatac
    49801 ggtcatcttg tatgaccttt ggtggaatcc tgccattgaa gaacaagctg ctggacgtgc
    49861 acatcggatc ggacaaaaaa atgtggtaga agtgtggcgt atgatctctg aaggaacgat
    49921 tgaggaacgc atggattcac tccagcaaga aaaacgcgaa ctgttccaaa aagtgatcca
    49981 aggtaatgaa gaacagttga cgaaactgac tgaagaagac atccgcatga ttttaagtgt
    50041 aggagaaatc gaggagtaag agactcgaaa aagcgttcag taatgggtaa gaaagccttt
    50101 ctaaagaagc gattgtttat ttgatttaaa aaatagcaag gaggctggga ttccatttta
    50161 tcccagcttc cttgctatca tttttttatt tctattttta aaggttatac aaagattgtc
    50221 ttcattgtct ctaacagctg atcaaaagaa gaaatctcga tatcgcaaat tggagtctgt
    50281 tcaattttgc gttctcgatg gttgaaccaa agcgcttgcc agttagcact ttttgcacct
    50341 agaacatcat tgtcgtaatt atcgccaacg tataaggtct cttctggaag catatgaaat
    50401 tctttctcag ctagctgaaa aatctctttt tctggttttt ggaaacctgt tgcttgagaa
    50461 atgagcatat tgcgggaagg aatccaattg ttcaattgaa gttgcttcag tttttttgtc
    50521 tgatggtcag tcggcccatt tgtgataatt cctaatggga cattcttttc cttcaagaaa
    50581 tcgagggttt ttttgacctc ctcatgcaag cagatattat caagttcttc ctcgtaaatt
    50641 ttttgaaata agaggccatc ttcttccgta atatgagggt agtctaaatc ttttagtgat
    50701 tggctgatcc gatgtgctcg catatattct agtgtccaat ctcctgccat tacttttggg
    50761 aagttttcat cactatgatg gcggaaccgg atatatagtg gatgcatatc tgcatctgaa
    50821 acaagcggaa cgactctttt gaccgcgttt cgaaatggct gctgctgatc atacatcgta
    50881 tcatcaacat caaacacaac tgatttaatc aaaattttac acgcctttcg gttttcattg
    50941 attttttcac aaatccttga ctattgtaac agaaaaaatt gtctaaaaaa gctgataaat
    51001 aaaggttttt cttactatat ttctatttcc ttgacagaat aaggttttta tatttcagtt
    51061 tttttcacta aaaatcgtga aacacgtcaa taagtttgat gaacaaatta atcattatgg
    51121 tagaagtcga ttgatttatt ttaatgcaat ctctgtggaa ttttcgaaag atttattttt
    51181 ttaatacagt ctcaatataa ctattcaaac ataaaataac gttatttgat ttatttgttt
    51241 atagtaaatt ttatttagta atcattgctt taaaatacta cgcttatata acagagaaaa
    51301 taagcgttat aataaagaac agtattattt aaaatgacag aaaacattac tattaaagaa
    51361 ataaaaaaca taaaaaatct taagaaatcg gtttcattta gcttttgaga taaaataata
    51421 gtacagtttt atacgctgtt tcatatgttt agaaaatttg aattagattt gaaaactcta
    51481 atttttaaat gataaaaaaa gcgagaatgc tggttttact gtatattaac cttagataac
    51541 tttcattttt ttaaaaaaat tatgaaaaaa aggtagctat ttaaaagttg attatgtata
    51601 atagaaacag atgggagatt attatgatct tctgttacaa aaaagtgatt cttatcacga
    51661 aaaggagtgg tgcacgttgt taacattata tacttcccca agttgtactt cctgcagaaa
    51721 ggctcgtgcg tggttgcaag aacatcagat tccatttgtt gaacgtaata ttttttcaga
    51781 accattgaac agttctgaat taaaagcgat tttacaaatg acagaagacg gaacagaaga
    51841 aatcatctcg acacgttcca aagtattcca aaaactgaac atggatttag atgatctgcc
    51901 gttacaagaa ttattggaac ttgtgcaaaa caatcctgga ttgcttcgcc gtccaatcat
    51961 gattgatgat aaacgtctgc aagttggttt taatgaagat gaaattcgtc ggttcctacc
    52021 gcgcgatgta cgacaattag aattacgtca agcacaattg atggctggat tatagaagga
    52081 taatacgaaa cttggaaaag actattacaa agatagtttt tccaagtttt tttgttttta
    52141 caaaaagtat tcttgattag atgtataaaa aggtagtaga agatgatgat tgcacgaaaa
    52201 atagagaggg aaaattagct gaaaagtcgg ctatatagta ttcgtctgcc aaaaatgtta
    52261 tagttaaata agatagcttg aaaggttgaa aaatccgctg gttttattaa attcgcttaa
    52321 cagcgagatt ttttttcttt acatttaagc tgatttcgct acaatgaatc tatattaaac
    52381 attgtggcaa agtgaggtgt agcaaaatgg aaatggaaca tattaatgaa aatacgatcc
    52441 gtgtactaat tggtcatgaa gatttagagg aacggggcgt ttcatttttg gatttacttg
    52501 gcaatcacaa agaaatcgaa aatttctttt atagtatttt ggaagaagtt gatatcgaag
    52561 aggagttccg aggaagcgaa gctgtcactt tccaagtttt gccaaaagga gacggattag
    52621 aactgtttat cagtaaaaat cttccggatg aagaaatttc tcaatcagaa gattctactg
    52681 ttgattcaga agatgtgagt gactttcttc gtcagcagat tattggcagc gatccggagg
    52741 aagaattatc tggaagcgcc ctagaagaac aggaaagaca agcaactttt gcttttgaag
    52801 attttgaaca agtgatccaa cttgcaacag atgtcgaact agagtctgct tggactgatt
    52861 tgtatttatt cgaagagcgc tattacctag tcgttcactt ctggatggaa aatctcaacc
    52921 aagcggatgt agagaatcag attgctcgaa ttttggagtt tgctgaaaag agcgatcgta
    52981 caccagaaac attgaatgaa tatggacaat gtctgatggc gcgtaatgct atcgagcgaa
    53041 cgaaatttta ttttgtataa atcagcatta tagtaagaga ctgtgacaaa aattgtgtcg
    53101 cagtcttttt ttcgtataaa tgtgttcaaa agcttgcatc tgcggtaaca agcccaacca
    53161 aacgaaaaat attgggaact atttttgttt agctgttctt attacttgat gcagaatagc
    53221 tttttcacgc cctctttttt cgtgtaaata aattcttaga cgtaaataag ttcagaggga
    53281 gaaaaatgct gcttgcaatg aatgaatata ataagtgctg ttttgctaaa catgcagaaa
    53341 aaggaaagaa atattattgt cgtggctgtc gaaaaagagt gattttgaaa aagggaaaga
    53401 aaaaatgtgc tcattttgcc catcaaaaat cagataactg ttcggtcttt agtgaaagag
    53461 aatcaaaaga gcacttacag ttaaaggaat gcttcatgga ttggctggga cagtcagtag
    53521 aaccagtatt tttggaagcc tatttgccga ggttgcggca aaggccggat attttgttgg
    53581 cgaatctagc aatagaaatc caatgttcta gactgtctca ccagagattt attgaaagaa
    53641 cccaaaatta tttgaataac agttaccaag tatggtggat acttggtaat tcttttcttg
    53701 gacaatcgca attctcattg atagaaaaaa gttgctgcta ctataataga aaaagaggag
    53761 tgcattgctg gaaagctgat ctcaagaaac aaaagcttta tttgtatcat catattacag
    53821 agactgtatc agggcatatt tccttttgca gtagctgctg gactttttct agtagggacc
    53881 ttaaagaaat attcactagt aacgaaatta aaatgaacca aatgaaaaaa atagaaaaag
    53941 tgagtgaaga tgggaagaaa tggctgacac gccaacttat tcacaagcaa aagaatacag
    54001 tatccataca agaacaatgc tacttgcgtc ataaacatct tttgcatcta tcatcgtgga
    54061 tctatcaaaa aagtcggttt ttcttttatt tacaagaaca ggtttttctt tatcgaatgt
    54121 tgtacgaaga agccttggaa aaccagaaag ttcctgattt caactcatgg ctttgccaaa
    54181 taaaagaaca taaaagggaa tggctttttc ctatgattga tgaagagatg gtctatcagc
    54241 agttttttaa cgaatgcata cacttgtctt ctttgaaaaa atagtaagta taaaaatagg
    54301 ctaggaatcg gttttctatg taaaacaaga tttagttatg ctaaaattga tggaagcaga
    54361 cacatgaatg agtagaaaag ttagatgctg tgacatgtgg atgttagact atgatctatt
    54421 ttgatagata gagctaaggg aagaaaagga gtggatttca tggaagtaaa gcagttgcca
    54481 aaacgagaag aattgcctga aaatttaact tgggacttga ccaagatctt ttcaagcgac
    54541 caagagtttg atgagaaata tttggaatta tcagaagagt taaaacaatc tgaaaaacac
    54601 aaaggaacac ttgatcaagg cgcttctcaa tttttaaatg cgattgaatt cgtattgagg
    54661 gtttatcgcc aaactgaagt catttatgta tatgcgcacc ttaaaaacga tcaagacact
    54721 ggaaatacag attaccaagc gctttatgca agagcaagca gtctgttttc gaaagttagt
    54781 gaagccgttt cctggtttga accagaaata ttgcaattgt cagatgacca gatttggcaa
    54841 tatttcaaag aagaaccaaa attggaagtc tatcgccatt atatccagca aatagtagat
    54901 aatcgagccc atgtcttatc tgctgagcag gaatctcttc ttgctggagc aggtgaaatc
    54961 tttgatgctt caagtgatac atttgctgtt ttgaataatg cagatctagt ttttccaacg
    55021 attgaaggag aaaatggtga aatagtccaa ttatctcatg gcgtgtatgg tcagttgcta
    55081 gaaagcacgg atcgaagggt gcgcgaagca gcatttaagg ggttgtacag tgtttacgaa
    55141 caatttagaa atacatttgc ttctacttta ggcacacata taaaaggaca taattttaaa
    55201 gcgaaagtcc gtaattacag ctctgccaga gaagcgtctt tgagcaataa tcatattcct
    55261 gaaagtgtat acgatacttt ggtagacgtg gtaaacaagc atttgccttt gttacatcga
    55321 tacatggaat tacggaaacg tttattagaa gtggaaaaac tgcacatgta tgatctttat
    55381 acaccggtct taggggaagc tccaattacc tttacgtacg aagaagcaaa agaaaaagct
    55441 ttagaagcac tgaaaccaat gggtgaagaa tacatggcca tcgtagaaaa agcattctct
    55501 gaacgttgga tcgatgttgt cgaaaataaa gggaaacgaa gcggtgctta ttcttcggga
    55561 agctatgaca caaatccata tattttattg aattggcatg atacgctgga tcagctattt
    55621 acgcttgtcc acgaaatggg acatagtgtt catagttatt tcactcgttc gaaccagcct
    55681 tatgtgtacg gcgactactc catcttttta gcagaaattg cttcgacaac gaatgaaaat
    55741 atcctaacgg agtatttatt ggaaacagaa aaagaccctc gtgtacgggc ttatgtactc
    55801 aaccattact tggatgggtt taaaggaaca gttttccgcc agacacaatt cgctgaattc
    55861 gagcatttca tgcatacgga agatgaaaaa ggtgtgccat tgactagtga atacctaagt
    55921 gatagttatg gtaaattgaa tgcaaaatat tatggtccag cagtcgaaga agacccagaa
    55981 attaaattcg aatggtcacg gataccgcat ttttattata attactatgt tttccaatac
    56041 tcgactggct tttctgctgc ttcagcactg gcgaagaaaa tactaaacca ggaaccagag
    56101 gcactagaga actacttggc ttacctaaaa gcaggtaaca gcgattatcc tgtggaagta
    56161 atgaaaaaag caggagttga tatgacacag gctgcatata ttgaagatgc aatgtcgatg
    56221 tttgaacaac gtctaaatga attagaagaa ttgatcgatc gtttataagg ctttctattt
    56281 ttgattttct cgaatagaaa cgaaaaaaat acgtcggctc tcttgtaagg gagtcgacgt
    56341 attttttta
//
LOCUS       DAMEET010000005        47695 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.005, whole genome shotgun sequence.
ACCESSION   DAMEET010000005 DAMEET010000000
VERSION     DAMEET010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 47695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.005"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            96..539
                     /locus_tag="PB780_000279"
     CDS             96..539
                     /locus_tag="PB780_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDE0732356.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(545..1729)
                     /locus_tag="PB780_000280"
     CDS             complement(545..1729)
                     /locus_tag="PB780_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HDE0732357.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            1997..2695
                     /locus_tag="PB780_000281"
     CDS             1997..2695
                     /locus_tag="PB780_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDE0732358.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2702..3379
                     /gene="nth"
                     /locus_tag="PB780_000282"
     CDS             2702..3379
                     /gene="nth"
                     /locus_tag="PB780_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDE0732359.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(3505..4038)
                     /locus_tag="PB780_000283"
     CDS             complement(3505..4038)
                     /locus_tag="PB780_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732360.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4335..6716)
                     /locus_tag="PB780_000284"
     CDS             complement(4335..6716)
                     /locus_tag="PB780_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDE0732361.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6706..7353)
                     /gene="recU"
                     /locus_tag="PB780_000285"
     CDS             complement(6706..7353)
                     /gene="recU"
                     /locus_tag="PB780_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HDE0732362.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7417..7956
                     /locus_tag="PB780_000286"
     CDS             7417..7956
                     /locus_tag="PB780_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HDE0732363.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8046..8465
                     /gene="gpsB"
                     /locus_tag="PB780_000287"
     CDS             8046..8465
                     /gene="gpsB"
                     /locus_tag="PB780_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HDE0732364.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8487..8854
                     /gene="rnpB"
                     /locus_tag="PB780_000288"
     ncRNA           8487..8854
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PB780_000288"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9063..10229
                     /locus_tag="PB780_000289"
     CDS             9063..10229
                     /locus_tag="PB780_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDE0732365.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            10275..11771
                     /locus_tag="PB780_000290"
     CDS             10275..11771
                     /locus_tag="PB780_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HDE0732366.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            11852..12535
                     /locus_tag="PB780_000291"
     CDS             11852..12535
                     /locus_tag="PB780_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDE0732367.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            13180..14370
                     /locus_tag="PB780_000292"
     CDS             13180..14370
                     /locus_tag="PB780_000292"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDE0732368.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            14472..15425
                     /locus_tag="PB780_000293"
     CDS             14472..15425
                     /locus_tag="PB780_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDE0732369.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            15494..16843
                     /locus_tag="PB780_000294"
     CDS             15494..16843
                     /locus_tag="PB780_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDE0732370.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVIAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(17026..17349)
                     /locus_tag="PB780_000295"
     CDS             complement(17026..17349)
                     /locus_tag="PB780_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732371.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            17478..18641
                     /locus_tag="PB780_000296"
     CDS             17478..18641
                     /locus_tag="PB780_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDE0732372.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            18711..20060
                     /locus_tag="PB780_000297"
     CDS             18711..20060
                     /locus_tag="PB780_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDE0732373.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(20105..20536)
                     /locus_tag="PB780_000298"
     CDS             complement(20105..20536)
                     /locus_tag="PB780_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDE0732374.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            20924..23566
                     /gene="alaS"
                     /locus_tag="PB780_000299"
     CDS             20924..23566
                     /gene="alaS"
                     /locus_tag="PB780_000299"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDE0732375.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            23766..24362
                     /locus_tag="PB780_000300"
     CDS             23766..24362
                     /locus_tag="PB780_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDE0732376.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            24484..25632
                     /locus_tag="PB780_000301"
     CDS             24484..25632
                     /locus_tag="PB780_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDE0732377.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(25670..26083)
                     /locus_tag="PB780_000302"
     CDS             complement(25670..26083)
                     /locus_tag="PB780_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HDE0732378.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            26162..26593
                     /locus_tag="PB780_000303"
     CDS             26162..26593
                     /locus_tag="PB780_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HDE0732379.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(26692..26925)
                     /locus_tag="PB780_000304"
     CDS             complement(26692..26925)
                     /locus_tag="PB780_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HDE0732380.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            27108..28778
                     /locus_tag="PB780_000305"
     CDS             27108..28778
                     /locus_tag="PB780_000305"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HDE0732381.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            28925..29311
                     /locus_tag="PB780_000306"
     CDS             28925..29311
                     /locus_tag="PB780_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732382.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            29298..29996
                     /locus_tag="PB780_000307"
     CDS             29298..29996
                     /locus_tag="PB780_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDE0732383.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            30001..30465
                     /locus_tag="PB780_000308"
     CDS             30001..30465
                     /locus_tag="PB780_000308"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDE0732384.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            30462..31373
                     /locus_tag="PB780_000309"
     CDS             30462..31373
                     /locus_tag="PB780_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDE0732385.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            31608..32147
                     /gene="pyrR"
                     /locus_tag="PB780_000310"
     CDS             31608..32147
                     /gene="pyrR"
                     /locus_tag="PB780_000310"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDE0732386.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            32161..33444
                     /locus_tag="PB780_000311"
     CDS             32161..33444
                     /locus_tag="PB780_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDE0732387.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            33457..34383
                     /locus_tag="PB780_000312"
     CDS             33457..34383
                     /locus_tag="PB780_000312"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HDE0732388.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            34402..35682
                     /locus_tag="PB780_000313"
     CDS             34402..35682
                     /locus_tag="PB780_000313"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDE0732389.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            35687..36763
                     /gene="carA"
                     /locus_tag="PB780_000314"
     CDS             35687..36763
                     /gene="carA"
                     /locus_tag="PB780_000314"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDE0732390.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            36768..39953
                     /gene="carB"
                     /locus_tag="PB780_000315"
     CDS             36768..39953
                     /gene="carB"
                     /locus_tag="PB780_000315"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDE0732391.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            40007..40783
                     /locus_tag="PB780_000316"
     CDS             40007..40783
                     /locus_tag="PB780_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HDE0732392.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            40780..41712
                     /locus_tag="PB780_000317"
     CDS             40780..41712
                     /locus_tag="PB780_000317"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDE0732393.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            41709..42425
                     /gene="pyrF"
                     /locus_tag="PB780_000318"
     CDS             41709..42425
                     /gene="pyrF"
                     /locus_tag="PB780_000318"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDE0732394.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            42432..43061
                     /locus_tag="PB780_000319"
     CDS             42432..43061
                     /locus_tag="PB780_000319"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDE0732395.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            43049..43363
                     /locus_tag="PB780_000320"
     CDS             43049..43363
                     /locus_tag="PB780_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732396.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            43401..44042
                     /locus_tag="PB780_000321"
     CDS             43401..44042
                     /locus_tag="PB780_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDE0732397.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            44085..44777
                     /locus_tag="PB780_000322"
     CDS             44085..44777
                     /locus_tag="PB780_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HDE0732398.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            44774..45676
                     /locus_tag="PB780_000323"
     CDS             44774..45676
                     /locus_tag="PB780_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDE0732399.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(45834..47540)
                     /gene="efbA"
                     /locus_tag="PB780_000324"
     CDS             complement(45834..47540)
                     /gene="efbA"
                     /locus_tag="PB780_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HDE0732400.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        15520 a         8350 c        10270 g        13555 t
ORIGIN      
        1 cttggtttca gattgggttt tcctacaaaa tatttttatc tttgttgaat aggaaacaaa
       61 aaaacaatat aatgaaagaa aaagaaggtg tagaaatgga taaaagacaa ctgctaatag
      121 aagaatatct ggatttagct aatgagatta ttcatcgtaa taaaccagaa atggaaaaag
      181 aatttgcagg atttactcta aatgaactag aagtgattga acatatcggg aaaatccctg
      241 atcctaacgt gacaaaatta gcgaatgcta gttacatgac tagaggagcc atcagtaagc
      301 taacaaaaaa actgatagct agagggatca taaacactta tcaaagcgaa gagaataaaa
      361 aagaaatcta ttttcaattg acgccaatcg gacaaaaaat aaggaataga cacgagcaac
      421 tccatcaaaa atgggtacag cgagatgcta ctgtatttga aaatatgtca aaagaagaat
      481 ttgacacagt atttcgtttt atcggtcgat acagagaatt tatacgaagt agcaaatgaa
      541 gctactattt tttattgtag tgataatagg caaaaatcat aagcaaaaat ccgaataccg
      601 tcggaagaaa tggtgctgca gcataaagct gtcctgcgat tagcgggccg atgacgcgcg
      661 atagagattg tatactttgg gtaccgccca ttacaagacc ttgctggtta tctgagacag
      721 aattggatag ttggccattg aatacaggag taaaaataga atcaccgaaa ccataacaga
      781 taatcccaaa taaaaacaaa atcggcagta gaagaattcc agataatgtg atgaatagat
      841 aggcgaggat ttcactgaac atccctattc ttgtgatctg ctgttctgaa aattttttga
      901 gcaatcgagg cataatgaac aattgcgaga cagtatccaa gattccaatc aaagagaagc
      961 ttaaaccaac caacccagcc ttccactgaa aggcgtcaat cgaaaattga gaaaagatag
     1021 attgaagaga gccagctgcc aaccaaacag caaaacctgc tgtcaataaa ggaataacgg
     1081 atgaaatctt gaataattgt tgtaattggt gaaaaggtct tacatgggag aaacttaaat
     1141 ctactgaacg tttttttatt ggaaggcttt ctagcataaa tgtgtagcca taaacggcat
     1201 ttaagaaagt aaagagtgct ccaataaata taggaacaga attccccaac tcagctaata
     1261 gaccccctat aataggaccc agcgttgttc ctataccgac taaagcaccc atccatccaa
     1321 aaactttggt tctttcatta ggttccgtga tgtcggcaaa gtaagcatat aaggtggaga
     1381 tttctcctgc cgtcaatcct tcaatgatac gaccaaggaa caacatccat atcgaatttc
     1441 ctaaaccaaa aagcaaatat ccgatacttg atcctaataa actgataata aggatcggat
     1501 gacgcccaaa atgatcactt aagctaccga gaatgggggc agataagaag gcagctagag
     1561 cataaacaga catgagcaaa gtgatataga aagcttgctg atgtatggag tgagaataag
     1621 gcagcgttaa aaacggaagt acaggactga cgatagtaaa tcctaatcca gtgaataaga
     1681 cagagacgta tccaaaaact ttagaatgtt tatttgtgaa actgtacatg taaaaactcc
     1741 tttatgcaat aatgttaact tggaaacaaa aatagtgtat agtaattttg tttccttgtc
     1801 aacattctaa ggaacagcga aatctactta atttatttat tccgaaaagt gaagatacgc
     1861 atcaaaaaca tgaacaaaca aaaatcaagt agacgacatt gtaaatctga ccgatcagtg
     1921 aagtaaatag aaaagaaacc tgttcttgct tcccatgttt tttttacgta tactgtgatt
     1981 gagcggagga gaaatcatgt tgaatttaga aaattactta agcgctggac aaacagtcat
     2041 atctaatctt ttacttaaaa attatcatag gatcggtcta caatctgaag aatttttatt
     2101 tgttctacag ttgcatatgg cacagcttga aggagatact ttccctgatt tgcagatgat
     2161 tgctatggat atgggaatca aacaagatca gatttttcaa atactggatc gtctcgtaac
     2221 aaatggattt atcaagatag aaacgacgat tgtcaacggt aaaaaagcag atcgatataa
     2281 tctttacccc atctatgatc tattaggtga gtatttaaaa acacaagaaa aaaaatacga
     2341 agaaaaaaca caagaaaaag aaatccagtc agtgtatcaa ttatttgaac aagaatttgg
     2401 acgcccgctt tcttcgatcg agttccagcg gatcggacaa tggctagaag aagaccatta
     2461 tcagccggaa attttgaagc tagcactgcg ggaagccgta ttaaatcagg cttatagttt
     2521 taattacgtt gaccgcattt tgctatcgtg ggaaagaaaa aatctacgta caaaacaaca
     2581 agtggaagaa gaccaaaaac gaagaaaaca gcagcttctt caaagagaag tagaacagtc
     2641 agctcaacaa gaagaattgc cgaaagtctc actgaaaaat tggttggagg agtagcggaa
     2701 tatgttaagc aaacaaaaaa caatggaagc tctagaaacg atgtatggaa tgtttcccga
     2761 agcacatgga gaactcaagc acaataatcc atttgagtta ttgattgcag tgatcttaag
     2821 tgcccaggcg acagatgtat cagtgaataa agcaacgcca gatttgtttg cctcttttcc
     2881 gacacccgat gcgttagcag aagcttcaat cgatgaaatc attcttaaaa taaaaacgat
     2941 tggtttgtat cgaaataaag caaaaaacat caaagcgtgt gcacagcagc tgattgaacg
     3001 tttcgatggg caagtgccaa cgtcaagaga agaattgatg tctcttcctg gtgtaggcag
     3061 aaagacagca aatgtcgtat tgggggatgc attcgggata ccagcaatcg cagtagatac
     3121 ccacgtggaa cgtgtctcaa aacgcctgag gatctgcaaa ctagatgcta cggtcatgga
     3181 agtagaagaa acactgatgc ggaaagtacc acaagaatta tgggtaaaga cacatcatac
     3241 actgattttt tttggtagat atcattgcac ggcaagaaat cccaagtgcg aggtatgtcc
     3301 tttgctatca atatgtcaag atgggaaaaa tcggatgcgt ctaaaagaaa aggcgctaaa
     3361 aaagaaacca cgcctgtaga cgtggtttct ttaattgcaa ataaatgtat gaattatctt
     3421 gttattatgg agaaaagctg tttcgtttat cttttttgca tgaaaaagta aatgtacata
     3481 ctcagtagct aggatggtaa cttattacaa tttattgcga taaaaataaa agttgttatt
     3541 tttattggga aaatacccga tgtagaaaat aatgccttcc aatataccta taaaataagt
     3601 ccagtattcc aaaggattat ataaataagt gacgtatcta tctaaaggtg taataaaaat
     3661 gataagtgat aataaaaaaa taccatttat aactaaacat aaactaatgg atttgggcgg
     3721 tttcaggctc atcagaaaaa tatagattct ggtgaacaac aaccataaaa aagtgagaat
     3781 cattgcatga gagcttcttt ccataaattt tcctttaaga gtagtgctta aaagtacaga
     3841 aaaaataata aatgcaagga taggagcaat taaagtaaat ttatttaata ataaacaaaa
     3901 tttttcagct tttttattct tggaataacg aggtattgtt ttcgataaaa aaagatggtg
     3961 cagacaaatt atgataatta agcatactat cagataactc aaaatgaagc cttctttcaa
     4021 aaaattattc aaaaccatat attgtatttt ttatttgata ataacaatgg gattattaag
     4081 aagtggcact aaataaaaga ctagtgtgta tttagccgat ttaattataa acaagtaaat
     4141 ggataaagta tatgtaatga taaaagaaaa atggattttc taatttttaa gctaaatgtt
     4201 agatcacaca tgtatatgct ggatagtctt ataaaaaatc atgtaataca gcttggtaca
     4261 tgatttttat ttgtagctgt tttttacata gacacaaaaa agaaaccacg cttgtagacg
     4321 tggtttcttt tttactatct gtttccagaa gctgaggtgc tgctggatgg tgtatggtca
     4381 tttgcagcat ccgctccgct agttgctggc tcactggaag aagcaggagg agcagatgaa
     4441 gacggtgtcg agctttcttc ggatgaagaa gaaggaaccg ttgttgaaga cgctggctcg
     4501 cttgtttctg ctgtcgtgct ttcttttgag gactcagcag ttgattcgct ttgactagta
     4561 gagctagagc tttgagtcgt tgtctctgtt gttgagcttc caggagtctg gacatagctg
     4621 gaagatggaa tagttgttga tggtgtaatt gcattcgatc tagctgtata ttgatctttg
     4681 tagtacagtt caccgcccac acgaattaat ccgcttggca tctcccagtc tgtatttgtc
     4741 acgttagctg aaacatactg cataagctcg cgataaacat cagaggctac gtgtgacgat
     4801 tctgaagtga caggtgtcat ttttttattg taacctgtcc acacggaaat cgaataattt
     4861 ggcgtatagc ccgcaaatag gatatctgga tacaccccag aagaaatacc aagttttgca
     4921 tactcgtcat ctgtgtaatt ggatgttccg gtcttaccgg cttgatataa cccagcgatt
     4981 tgcgcattcg taccagttcc ttcagtgatc gtatctttta aaatgtctgt gatcatataa
     5041 gctgtttctg gagacatggc tgtttttcca tctggctcat aagtttcttc tgttccgtct
     5101 tggaagacga ttttattaac atattgaggt ttgtaataag tccctccatt ggcgaaagcc
     5161 gcataggctg ctgccatttt taaggaagaa gctccgtatt ttgtcccatc ttgttcttcc
     5221 gtattactag agatcgcatt cgattgatgg atcgtactgt attcgatccc aagatttttt
     5281 aagaaagaag ccactttatc tgaaccgact tcattgaata atttgactgc tggaacatta
     5341 cgggataagt aaagtgcttg gcgtaaagta atggtgccca tatattgatt atcccagttt
     5401 cctacaggag tcgaagtccc ttcatagtta tacggtgcgt cagtaatagt ttttccagta
     5461 gaatatttca aatattcaaa agctggtccg taatctgtga caggtttcat cgtagaacca
     5521 aaatcacggg aagtattcac tgctaagttg ttgcctaaag ttacgtcttc tgcgatgtga
     5581 cgtccgccaa tctgggcttt tactttcccg gtattggtgt cgataagggt ggaggcaact
     5641 tgcatctcgt cgtctgggta agagacatac tgatccgtgt tgacgatatc atatagtttc
     5701 ttttgggcgt ccagatccaa attcgtataa atctctaaac cgtctgtgta gacattctta
     5761 tctgtttttt cttgtacttc attgatgact tctttgacat aattatcgac gatcttagta
     5821 ttgtcatcgg attgagttag ttcttgcaga ccatccgtta ccggtacatt aacagcttga
     5881 tcatattctg tttgagagat tttttcgttt tgaagcatcg tatacaaaac agtatctcgt
     5941 cttttcttcg cctgatctgg ataaacataa ggatcatagg cggaaggggc ttgaggcatc
     6001 ccagcaagca atgctgtttg cggtaaagaa agttctgata atttcttacc aaagtacatc
     6061 tcagaagctg tctccatccc gtacaagccg tttgacatat acactttatt tacgtagtag
     6121 gtcaagatct cttgtttcga ttttttttgt tccaatctta cagccatcca cgcttcttgg
     6181 gccttgcgtt tcagagtctg atcctcggca ctggtggaga agaaggaaag tttgatcaat
     6241 tgctgtgtca aagtacttcc gccttgcagt ccgccagagg taaagttaga gagggcagaa
     6301 ccgatgatcc gaatagggtc aaccccgatg tgtttataaa aacggcgatc ttctaccgac
     6361 acgatcgcat cttctaatgt tttcggtaat tcattcgcag aaattttttc acgcttttct
     6421 gcgcctaaat cttcgaatag ttcgccatct tgtgtatata gtttggaaga tacggtagcg
     6481 tctaattttt tatccgtcaa ttcgggcgca tcttttgcgt aataccagaa aagtcctaca
     6541 cctgctaaaa agaggataca gaaaaatgag agtattccta aaaggatctt gataaataat
     6601 ccttttttat gtgttccaga ggactttcca gaacctttgc cagaattctt cttcacactc
     6661 tttttaggag taggctgttt ctgtcgtcta gagcttctag tttgttcatt tgccattctt
     6721 gctttctcct tcaatagata atgttgataa atagaagtca accgcgtcca aataaggaat
     6781 ccgcggagaa ataccagttt tgatctctat tcctagttct tcgatcaaag ataaaggtag
     6841 tgatttgcgt cctttttcct tttgggcgtc ccagtaagca atcaaatgaa cactattcaa
     6901 ataaaaacag cgcttcaaag acgaaaacca taacaagaca aagcagaccc cattttgttc
     6961 gaggcatgct ttcatatgtt cgatttgatg ggcatggaag tttttaaagg gaaaagatgt
     7021 tttgttcttg gtttcttttg cttcaaagtc caagtaaaag cctcgataga cgccattata
     7081 gtctgtagtt gaggcttggg taaagtaggc ttccttgatc acagcagcac ttctcttagg
     7141 atagtctact ttcacgattt ggataggggt aggcttttta tggataaccg ccaacccgcg
     7201 ggaaagataa tatttattac tttcattgat tgcttcttca aagcgcatcc cccgattgcc
     7261 gaattcagtg atctgatttc ttttttcttg aattttttcg acgggagagg cattcttata
     7321 ataaggttgg ccatttggat agcgcaatac catctttaat cacactccta ggccacatta
     7381 tagcaaaaaa gcggatagaa agaaaaggaa tctgagatgg ataagtcaaa agtaatctat
     7441 gtgtctgggt atcgaagttt cgaactcggt gtttttaaag aaaatgatcc taaaatccaa
     7501 gttgttaaaa atgttttaaa aaaagaactc catcagttag cagaagaagg gctggaatgg
     7561 gtacttgtca gtgggaattt gggcgtcgaa ctatgggctg tgcaaatagt agcggaattg
     7621 aaagaagaat atccggaact atctcttggt attttgtatc ctttttctga ttttggtaat
     7681 aactggaatg aagggaacca agaaaaaaaa caattagcag aacaattagc agattatgtc
     7741 gaagctgtta gccatcaacc ctaccaatca cctagtcagc taaagaatca cactcgtttt
     7801 atacttgagc atacagtggg ctgtctgctg atctatgatg aagaattccc tggaaaaacg
     7861 aagtattttt ttgaagatgc acagaattac caagcagatc atccctacga aattcggctg
     7921 atcagtatgg acgatcttca aaattttagt gaataatcga tattgtgttt gatttatcaa
     7981 cttttttcct atacaatgaa aaaggtagaa atgttattac gtaaatgaaa actagaggtg
     8041 taaatatggc gaatttggtt tatagtccta aagacatttt acaacaagaa ttcaaaacaa
     8101 aaatgcgcgg ctatgatccc gttgaagttg atgaattctt agacaatatc atcaaagatt
     8161 atgaaactta cagcaaagaa ctccttgctt tacaagaaga aaacgatcga ttgagtgcaa
     8221 aagtagctca attatctaaa actcaaggag cagctcaaac acgcgtgcaa caaacagagg
     8281 caccaaagag cgcagcagtg accaattttg atatcttaaa acgcttgtca aatcttgaac
     8341 gtgaagtatt tggtaaaaag cttgatcaac aagcaagtgc agtcaaacca gcacaaccta
     8401 atccaaataa ctacacaaat gcagacacaa gcttagatga taatgaaaaa acacgtcaat
     8461 tttaattttt gattaatgaa tttgcggttt tcgggtaatc gcggtctgac ttgatcaggc
     8521 tgaggaaagt ccatgctcgc acaggctgtg atgcctgtag tgttcgtgct tagcgaaacc
     8581 ataagctaag gtactctttg gagtaacggc aggaaaacaa gctaaggctt cggctatgct
     8641 taagtatcct tgaaagtgcc acagtgacga agcggctggg gaaacccatc cggtggaacg
     8701 cggtaaaccc ctcgagcgag caacccaaac aatggtaggg gcgctttttg tctggaagaa
     8761 gaacaggaca aaaaggcagt ataactgcag atagatggtt accgctttgt tttttctccc
     8821 tggaaaagca aagtacagaa catggcttat agaaaaccgc aaacatcagg gaattaggtc
     8881 gtgacaaaag taatgcgtta taagaaacaa gaaaaacatt tgaaagctat ttttcaatac
     8941 ttcagatgaa tcgttcttat tacaaaacaa acttttgtat caccggttat tcggaatttg
     9001 agtaaaagtg tggggtagga ctgatgtccc acctcctttt tttataaata aggagattgt
     9061 gtatggaaaa aaacaggaag ttcaacctcg tcgcaactgc ggcaagcggg ctagaagcac
     9121 tggttggtaa agaattaaga gatcttggaa tcgaatgcca agttgagaac ggacgtgcac
     9181 gttttgaagg aacaatggaa acgatcgcta cagctaattt atggttacgt acagcagatc
     9241 gagtaaaaat cgtggttggg gaattcgatg cgtatacgtt tgatgaattg tttgaaaacg
     9301 taaaagcatt gccttgggaa gattttcttc cactagatgc agcttttcct gtagctggaa
     9361 aatcgatcaa gtcgaaacta tatagcacac ctgattgcca agcgatcaca aaaaaagcaa
     9421 tcgttgaacg tctccgtacc tattatcacc gtccagcttc tgtaccactg acggaaactg
     9481 gcgcacattt tcagttggaa gtagctttgc tgaaagacca tgtgatggta actctggata
     9541 cgacaggacc aagtttattc aaacgtggtt atcgcttaga aaaaggtgga gcaccattga
     9601 aagaaaacat ggcagcagca cttattatgt tgacaaattg gcgaaaagac cgtccatttt
     9661 atgatcctgt atgtggttca ggtacgcttt gtattgaagc agccttaata ggccataata
     9721 tcgcaccagg attcaatcgt gagtttgctt gcgaatcttg ggattggttc agtcaagaaa
     9781 tcatggaaaa tgttcgatca gcagccgaag aaaaagcaga ttatgatatt gaattagata
     9841 ttactggttc agatatcaat ggacggatga ttgaaattgc caaagccaat gcagaagaaa
     9901 tcggattagg ctcttcgatt acctttaagc aacaagcagt caaagacttc aaaacggata
     9961 aagaatacgg ggtcatcgtg gctaatccgc cttacggaga acgtctagga gaagaagaaa
    10021 ctgttcggaa attatataaa gaaatgggcg atgtattccg tccattaaaa acatggagta
    10081 aatatatctt gactagcgac ttagcgtttg aggaatttta cggacaaaaa gcaaccaaaa
    10141 aaagaaaatt gtacaatggt gcattgcgta cggatctatt ccagtattgg ggaactcgtc
    10201 cgcctagaaa aaaagagaaa gacgaataat cttactaatt gattcattca attggatgtt
    10261 tggaggaata gggaatgaaa gaaaaagagt ttttacaaga agtaaaagaa atcaatcttc
    10321 tacaacaagc tttaggaatc ctcgattggg atacacaaac aggtatgcct gaaaaagcta
    10381 gtgcataccg aagtgaagta gacagctatc tttacagcat ttatttttca aaaaaaatcg
    10441 gtccaaaaat caaagaagcc attcgctatt tttctgaaca tccggaagaa ttgtcggaag
    10501 ttggaactgc cgtttttgcc aaagtaaaag aagagtatga tttggaacat cgcgtaccgg
    10561 aagatcttat gagaaaatat acagctgccg tttcttcagc acacggacaa tggcaaaaag
    10621 ctagagaaac acaacaattt tctgattttc aacacgcatt gaatcaaaat attgaattaa
    10681 caaaacagtt gataccttac tggagaaaag atgaaaagac gaattatgat gttttgctga
    10741 atcaatacga acccgggatg actgtcgaaa tccttgataa ggtatttgat cagcttaaag
    10801 aaggaattct ttcgatccgc cgtacgctcc aagaaaaagg gactgaacct gaaacagact
    10861 ttttaaatcg gagaatgacg aaaaaacaac agaagagatt tgtagtgaaa gtaatcgaac
    10921 aacttggtta tgattttagt cgtggtcgtt tagatgacac tgtccaccca tttatgactg
    10981 gaattaatcc gaatgatgca cgaatcacga cacgatggaa cgaaacagat ttcaaaatgg
    11041 ctgtttttgg agtgatccac gaggctggtc atggtatgta tgaacaagac atcgatgata
    11101 aatatgcgta tacaccggtt tatgaaggta catcaatggg tatccatgaa tcacaatcgt
    11161 tgtttaacga gatcattatt ggaagtagtc gcagcttctg gaaaaagcaa tatcctttct
    11221 ttcaagaatg tgcagaagga acattcgatg atattccttt tgacaaattt tatcgttcat
    11281 tgaaccatac aaaatcaagt ttggttcgta tcgaggcaga cagtttgact taccctctcc
    11341 atatcatcat tcgttatgaa atcgaaaaaa tgattttcaa tgaaggggta gatgttgctg
    11401 aactacctga aatatggaat caaaaatatg aagagtatct gggaatacga ccaacaaatg
    11461 atgtggaagg catcctacaa gacgttcatt ggtcaggtgc aagctttggt tattttccct
    11521 cttatgcatt agggtacatg tatgcggcac aattacttca ttctttgcag caagaaatag
    11581 atgtggaaaa tgttctagcg tcagacgatt attctccaat caaaaactgg atgtcagagc
    11641 atatccatca atatggtgca tctagaaaac caaatcaatt aatcatggat gcaacacatg
    11701 aaccgcttaa tccgcaatac ctgatcgact atatgaaaga cctgtatttt aatgtatatc
    11761 aggtaaaata aaatacaatt tacaaagata gctgaaattc atctaatcta tagatgtttt
    11821 cagctattta cttaagaaag aagtggaacg aatggataaa ttacaagtga tcgctgatta
    11881 tagtatggac caattagcgc atgatcaaac aggacatggg agcgatcata ccaaaagagt
    11941 tgtcaagtta gctgaaaaaa ttttggagac ggaaccgcag gcagaccgct ttgtgacgct
    12001 tgcagcagct tacctacatg atacgattga tgataaagtc gttaaaaatg agaacgaggc
    12061 aaagcaacag cttcgtgtat tccttcgctc tttgccaata gtagaggaac agatctcgat
    12121 gattttttcc attatcgaaa atatgtcctt ttctaaaaat ctctctgaag cggccgaact
    12181 atctctggaa ggtaagatcg ttcaagacgc tgatcgtatc gaagcattag gagcaatcgg
    12241 cattttgcgt actgcttatt tcggcggtgg acatgggcac ccgatttttg atcctgaact
    12301 gtatccgcaa acattcaagg acaaagaaaa ttaccgaaaa ggcacgacag tgatcaatca
    12361 tttttatgaa aaactgttct tgctgtctga taaaatgaat actgattacg gatatgaaga
    12421 agccaaacga agagaaacct tcatgcgtga attcttagaa gaattttttc tggaatggtc
    12481 tattgacgat gaaaatttta ctccagacaa ttggatcaaa aataacaaaa aatgatggag
    12541 gttgtgacag aagtggtcag ctccaagata tttcggatca ttttccgtaa aagttacttc
    12601 tggaacactg tttaccaaga ggtcgtgagc ctgccgttca gctccgagtc acaaggaaca
    12661 atttcaaaaa atagcttctc gtgtttgatg aatattgtaa cttgtgattc ggaattggca
    12721 ggagaacacc gtttattcgg aagaatagga ctgtgacaag acttttgtca cagtcctttt
    12781 ttcgaattca acaagtaaaa cttttaagaa atattcagag agtaaaatta aaaattgctg
    12841 ataaacgaag atggaaattt tatgtatttt tattggtcta tgcaataaat aaattaaata
    12901 ttcagaatac aattgatttt aacgaaaggg cttctatcaa tggttgtgtt gtatttatct
    12961 gcccaggtag agtaaaatag atcattatct aaaaggtaat tgttcatact atataagtca
    13021 tttgaaagta aaaagatact gtaaataaaa aatgtggtta aataattctc ataatcagtg
    13081 taagcgttat caaaaatata gcatacttgc tttgtagatg aatgacacga tatatgtttg
    13141 gtatgttctg caagacagat gaatatggag gtggagacaa tgtcttccaa agaactaaca
    13201 atgtatttag agaaagaatt ggatcaactg aaagaaaagg ggttatacaa tacgatcgac
    13261 atactagaaa gtgaaaatgg tgcttgtatc atcgtagacg gaaaaaagat gatcaatcta
    13321 gcatccaaca attatcttgg atttgccaat cgcgaagaat tgaaaaaagc atgtattgaa
    13381 gcaaccgaaa cttatggtgt aggagcagga gcagtccgaa cgatcaatgg ttcgctgaaa
    13441 atccatcagc aattagaaga aaaaatcgca gaattcaaag ggacagaagc agcaatcgcc
    13501 tttcaatctg gattcaactg caatatgggc gctatctctg cagtaatgac aaaagaagat
    13561 gctatcttat cggatgaatt aaaccatgca tctattattg acggttgccg cttatccgga
    13621 gcaaaaatca tccgaattaa acatcaagat atgaaagacc ttgagaaaaa agcaaaggaa
    13681 gcgatagaaa gtaagaaata taaaaaaatc atgtatatca cagatggtgt tttctccatg
    13741 gatggagaca ttgcacgatt gccggaaatt attccgattg ttgaaaaata tggactgatc
    13801 acctacgtcg atgatgcgca cggctcaggc gtaactggaa aaggtgcagg aaccgtcaag
    13861 cattttggat taagcgacaa aatcgatatg caaatgggca cgttatcgaa agctataggt
    13921 gttgttgggg gttatgtagc tggatcgaaa acactaatcg actggttgaa agctagaagt
    13981 cggccattcc tattttctac ttcgctaact ccaggtgcag ctgcagctgc attggcttcg
    14041 attactttga tgcaagaaca tccggaactt gtcgaaaaag tttgggaaaa tgctaattat
    14101 tttaaagaag aattgaaaaa agtaggctat aacatcggta tgtctgaaac acctattact
    14161 ccagtcattt tgggagacga aaaagtgact cagactttct ctaagaagct gatcgaacat
    14221 gggatttatg ctaaaccaat cgtttatcca actgtgcctc ttgggacagg caggatcaga
    14281 aatatgccga ctgcggaaca tacgagggaa atgcttgatg aagcagtagc agtttatcaa
    14341 aaaataggaa aagaaatgga aattatttaa ctaaatttag ttttttcgtc tatttaaaat
    14401 tagagaacat gttcacgcat atattgttaa tcatctgaag atagtagtaa aaaagatcag
    14461 ggaggatcat catgaaaaaa gtattggtga caggatgttt aggacaaatt ggttcagagt
    14521 tgatcacacg gctacgtcaa gaactaggca gtgaaaatgt cattgcgaca gatatacgca
    14581 tgcctgaagt cattgagaaa aacgatatct ttgagatcct agatgtaatg gattacgaaa
    14641 aaatgcgcag catagcagaa caatatcaag tggacacatt gattcatttg gctgctcttt
    14701 tgtcagctgt tgcagaagca aaaccacaat tagcctggaa tttgaacgtg actgggctgg
    14761 tcaatgcatt ggaagtcgct agagaattcc agctgaaatt ttttacgcca agttcaatcg
    14821 gcgcgtttgg tccaaatact ccaaaagatc ataccccaca agatactctt cagcgtccta
    14881 caaccatgta tggggtaacg aaggtagctg gagaactact atgtgattat tattatgtga
    14941 aatatggcgt agatactcgt ggtgtccgtt tccctggatt gatttcttat gcaaccttgc
    15001 caggaggcgg tacgacagac tacgcagtcg atatttatta ttcagcaatc aaagaaggaa
    15061 agtacactag tttcattgat caaggtacat caatggatat gatgtatatg ccagatgcga
    15121 tcgatgccat tataaaactc atgaatgcag atcctgatcg attggttcat cgaaatgcct
    15181 tcaatatatc ggcgatgtct tttgacccgg aagagatcaa agcatctatt caaaaaatca
    15241 taccagattt tgaaatggat tatgatgtgg atcctgtacg tcagtcaata gcagaatctt
    15301 ggccaaattc actagatgtt tcttgtgcgc aagaggaatg gggcttttcg ccgaaatacg
    15361 acttagatgt gatgacaaag gatatgttgg aaaaactgaa agaaaaattg gtagagacgg
    15421 tataaaacaa gaaccacatc caaaaagttt tttatacttt tgggattggc tatggtatga
    15481 aaggggacaa gggatggaag aatatcaagc cacgccagta aaagaagtaa aaatagaaaa
    15541 tgaattaaaa cgtacgatgg gattttttac tgctttatcg actgttatgg gcacagtgat
    15601 cggtgcaggg gtatttttta aagccgcaag cgtagcagaa gttacaggat ccgccagttt
    15661 gcatatgttt tcttggtttc tcggtggtat gatttctgtc tgtgcaggac taacaggagc
    15721 tgaactggca gctgctattc cagaaacagg agggatgatt aaatatatcg aacggattta
    15781 cggaaataca gcagcttttt tattgggctg ggcgcaagtc gtgatttatt ttccagcaaa
    15841 tgtagcagcc ttgtcgatca ttttcggtac acagttcgtc aatctatttg gtctttccca
    15901 gtcaatgatt gttcccgtag ctgttacagc tgctgtatcg atcctgttga tcaatttttt
    15961 aggttctaaa gctggagggg cttttcagtc gattacgctt gtctgcaagc ttattccttt
    16021 gtttgtgatc gtcattttcg gtttgttccg acaagaagga gtagacttcc aactgtttcc
    16081 gatccaagca ggagaaaatc tttcattttt ctcagccttg ggagcgggct tgctagcaac
    16141 gatgttcgca tacgacggtt ggatacacgt aggaaatatt tcgggagaat tgaaaaagcc
    16201 ggcgaaagat ttgccaaaag caatttcttt aggaatcatc ggaatcatga tcgtttattt
    16261 attagtaaat gcggtgttct taagaaccgc atctatcgac ggagtcgcag gaaacagcaa
    16321 tgcggcaagt gacgttgcaa aaatgatttt tggcggcttc ggtggaagac tggtcacagt
    16381 tggtatcttg atttctgttt atggaaccat caatggctat acccttactg gaatgcgtct
    16441 cccttatgtt attgcaaaag aaaacaacct gccttttagc aagctctttg ctaaacttca
    16501 tgataaaact aaagttcctg tagccgcagg tatcttagag ctagtcatcg ctattggcat
    16561 gatgatgatc ggtggttttg atacactaac ggatatgctg atctttgtca tttggatttt
    16621 ttatacgatg gtctttgttg gtgtcattct tcttcgtaaa aaagaacctg atttgtttcg
    16681 accatacaaa gttccaatgt atccgtttat tccactagta gcaatcattg gagggacttt
    16741 tatcgtatca agcacgctta tcactcagac ctttttagct tcgatgggga tagtattgac
    16801 acttgctggt atcccgattt atctctattt aaaacgtaga tagctaaaac attcgaattt
    16861 tattagaact gcacttctct gccaaacaaa atattttatt taaatgagga gtacgtggaa
    16921 gaaatgtcaa aagtactcgt taattttgat cacgtaaatg tcgttttgtc tacgatgaaa
    16981 tagcaaagta aaagacagaa atcgggctca accgatttct gtcttttact ttgatttgcg
    17041 taactgaact attcggcgta cgccgcgata aatatttaat aaaaccaagc aaccagaaat
    17101 gaaaatggga agccaaatca gatagacagg gtagccaagc actttagaca aagcatgtgg
    17161 agtcatttta tccatttcaa gaagtgcttg ctgccaagtc tgaagattga agactagcat
    17221 gaaacccgaa ataatcagga gaataaatcc taaataatga attgtagttc tttttcttac
    17281 ataagcgatc gtagcataaa ggaacagtaa aaaaataggt acgccaaaaa caaagtacat
    17341 attcagcatc tcgacacccc ctttgtgttt atcttacttt actttgaaag aaatgagaaa
    17401 taatagcctc gttacttgct tgaaacagca aaggactgct aagataagag agatacaaag
    17461 aatcaaggag gagtcatatg gcaagttata tcgaaccgat tcgttttgcg ctgattgttt
    17521 ttccattttt agccttagcg atctcatctg ttttttttat ttatgaatat cgaaagtatg
    17581 gaacatttct agtaacaagg gcagttattt tgtattcttt cgttttttat ttattgtgtg
    17641 cgtatttttt ggtgatctta ccacttccgg caaaggagac ggttgcccaa atgacagggc
    17701 caaagatgga gctgcattta tttgcttcat ggcaacattt cagagacaac acggtattgg
    17761 tcttgtctga acctagtacc tacctgccgg caatgaagca gagtgtattt ttggaaccag
    17821 cttttaatgt tttattactc ataccatttg gcatttattt aagatattat ttccggcttt
    17881 cttttatgaa aacgatgatt gctagtttct gcctttctct attttttgaa ttgacgcagc
    17941 taagtggact ttattttatc tacccaagac cataccgctt atttgatgtg aatgatttag
    18001 tgacaaatac attgggtgga atcattggtt ttgtcgttac gccgttattt acatttatgc
    18061 ttcctacaag aagacgaatg gatgaacttt cttatgaaaa aggaaagact gtctcattga
    18121 ctcgacgctt ggtcgcttat ttgatcgact ggacattttt atctcttatt aatttggtag
    18181 cggcgatttt ctttaaaatt gcgttcgatt caggggcagt tacttctcat atctggtggc
    18241 ttttattaga agtaggggta tactttatga ttgtaccgtt tataaccaat ggatggacac
    18301 ttggcaaaaa aattgtgcgt atccgtttgg tggaagaaga ccgcgaacga atttctttaa
    18361 aagcattgat gatccgctac gggtatctgt actttctttt ttatggttta agctggttgg
    18421 ggcagttgct tcagcaaatc atgaattcga caacgaatac ggtgttgcag atgagtgcag
    18481 tattcatttc tctcggtgtc ttgttactac aaggtatttt tattttaaat atcttgctat
    18541 catttttgag aaaaaaacgg attctgtttt atgaaaaagc tagtcacaca tatactgtta
    18601 gcacgattgg ccaaacaaag gatgatgatt ctcgaaatta gctgagcaaa aacgctttgt
    18661 ctgtgttata atgccaaagt cgtgataatt taaagagaag aggtgcacga atgaataatt
    18721 ttgatatatt ccgcttcaaa ccgtttatca atgaagcgtt aaccgcaaag ggcttcaaac
    18781 agcccacaga ggtccaggaa cgtttgattc ctttaattgc aaaaggaaaa aatatcattg
    18841 ggcaatctca aactggtaca ggaaaaacac atacgttttt gttgcctttg tttgaaaaaa
    18901 tcaatgcaga aaaagaggaa gtacaagttg ttattacagc tccaagtagg gaactagctg
    18961 cacagatcta tgaagcagct acacaaatcg ctaaattttc tacaccggag atccgtgtct
    19021 cgaattttgt cggcggtaca gacaaacagc gtcaattaaa tcgtctgcat caccaacagc
    19081 cacaggttgt agtaggcacg cctggtagaa tattagacat gatgaatgaa caagcgctga
    19141 agattcacac cgcatttgca tttgttgtcg atgaagcgga tatgacattg gatatgggtt
    19201 ttttaaatga agtagaccaa attgcaagtc gattaccaga aaagctgcag tttttagttt
    19261 tttctgccac tattccagaa aaacttcgtc cattcttacg aaaatacatg gaaaatccaa
    19321 tcattgaaga aatcaaacca aaagcagtta tttctgaaga cattgaaaat tggctgattt
    19381 caacaaaagg taaagatgag aatcaaatga tttatcaact gctgacaatt ggtcatcctt
    19441 atttagcaat cgtatttgcc aatacaaaac agcatgtgga tgaaattgcc gattacttga
    19501 aaaaccaagg cttgaaagta gcaaagattc atggagacat caccccaaga gagagaaaac
    19561 gagtgatgcg acaagtacag aatctggagt atcaatatgt tgtagcgact gatttagctg
    19621 ctagagggat cgatatcgaa ggtgtctctc atgttattaa tgcagaaatt ccagaagatc
    19681 tagacttctt tatccatcgt gtcggacgta ctggacgtaa tggcttaaaa ggaacggcca
    19741 ttacgttata ttcgccaaat gatgagcaag ccattgatgc gatagaaaaa ttgggcgtct
    19801 catttgaacc aaaagaaatc aaaaacgggg aaatcgtcaa aacatacgat cgtaatagac
    19861 gtgccaaacg agaaaaatca aaagatgtat tagatcccac gctgattggt cttgtgaaga
    19921 agaaaaagaa aaagatcaag cctggttata agaaaaaaat cgattgggca attgcagaaa
    19981 aaaataaaaa agaacgcaaa atcgaacgca gacagcaaag ccgtactgct cgtaaaaata
    20041 agaaaaattc acataaataa aaaacaagag gttgtgacaa aaggcgtgtc acaatctctt
    20101 gtttttaagc tagacttttt ttcattccga tacaaagtac ttcatcttca ataaaaggtt
    20161 ctgtgcaagt atgataccct tgtttttcat aaaaatctac agccgtcatt tcagctacta
    20221 cataactagt ctgacaacct tctgaaaccg cccgtttttc agcagcttca agcaactgac
    20281 cgcctaaccc ttttcctcgg tattcttttg ctacgcaaaa tcgttccggc ttgaagcaag
    20341 taggtgtttt cttttcataa cgcaaagtag ctactggtct tttttgatca agaaatagat
    20401 aataggtttg ttgcgggata tcttttccgt cgaattctag ttctgcagga atcccttgtt
    20461 ctaatacaaa aacttgatat ctcaaaaagc agctagctgc gcgtatccat tcatccgctc
    20521 caactatttt tttcatcata ttcctccttg tcatgaatag cagagatgta ttgattcctt
    20581 tattcttatc gtaccaaaaa tcgtggagta tgaccaaaag gaaatgaaaa taaaaattga
    20641 gatattgaca gtgatcaata gaatgcgtat actaattaag tacgaaaagg atatgatttg
    20701 ttttttaaga ctcacagaaa attctttctt ggctgaaaaa agaatagtca ctaatcaaat
    20761 tgctcccttt ttctattttg gtagaaatac cgacgcagct cagcgttatc gagtttaaga
    20821 ggtaatgatt acacatcatt gcaatcaagg tggtaccgcg agttttcgtc cttggctgtg
    20881 atggtgtttt tttgtattta atttatagaa agcaggtaat gacatgaaac aattaagtag
    20941 tgcacaagtt cgacaaatgt ttcttgattt cttccaatct aaaggacata cgattgaacc
    21001 tagtgcgtct ttgatccccg tgaacgatcc aaccttattg tggatcaact ctggtgttgc
    21061 aacattgaaa aaatattttg atggttcagt tgtgccagaa aatccaagaa tcacaaatgc
    21121 ccaaaaatct attcgaacaa atgatatcga aaatgtcggt aaaacagcac ggcaccatac
    21181 gatgtttgag atgctgggta atttttcgat tggtgactat ttcaaaaatg aagccatcca
    21241 ctgggcatgg gaatttttga ctgatccaaa atggatggca ttcgatccag aaaaacttta
    21301 tgtaacagtt tatccaaaag atacagaagc aaaacgcatc tggcatgaag aagttggcct
    21361 gccactagat cacattattg atgtagaaga caacttctgg gatatcggtg caggcccatg
    21421 tggtccggat acggaaattt tctatgaccg tggagaagct tataacgatt tagcagaaga
    21481 cgatcctgaa aactatccag gaggagaaaa tgaacgttat ttagaaatct ggaacttagt
    21541 attttcagaa ttcaaccata agcctgataa cacatttgaa ccacttcctc ataaaaacat
    21601 tgatacgggg atgggattgg aacgcatggt ctcaatcgtt caagatgcgt caacgaactt
    21661 tgagacagac ttgtttatgc ctattatcca cgccgtagaa aagctaagca aacaagtaaa
    21721 atatggtgaa aacccagtaa cagatatttc ttacaaagta atcgcagatc atattcgcgc
    21781 cctttcattt gctattggcg acggtgcatt gccatcgaat gaagggagag ggtatgtctt
    21841 acgccgtttg cttcgtcgtg ctgtcatgca tgggaaaaaa ttaggtattg aacaagcatt
    21901 cttatatcgt ttagtccctg ttgttgggga aatcatggta agttattatc ctgaagtact
    21961 tcaacaaaaa gaatttattg aaaaagttat ccgtacagaa gaagaacgtt tcaatgaaac
    22021 aatcaatgaa ggtttggaaa tcttaaatca agtgatccaa gaagtaaaaa acgaaaacgg
    22081 gacgactcta gcaggaaaag acatcttcaa actatacgac acatatggct tcccggtaga
    22141 attaacggaa gaggtggcag aagatgaagg actgaaagtt gatcatgctg gttttgaaaa
    22201 agaaatggat gcacaacgcg aacgtgctcg ctctgcccgc agtactgaaa aatcaatggg
    22261 ggtacaatct gctttattga cagatatcaa agtagaaagt aaattcattg gatacacaga
    22321 acaagaatca gatagtcaat tattggtcat cgttaaaggt gagtctcttt tgaatgaagt
    22381 cgcagaaggt gaagctcaaa tgatctttga tcaaacacct ttctatgcag aaatgggtgg
    22441 gcaagtagcg gatcaaggat ggatcatgaa tgaagtaggc gaaatcgtag cagttgttac
    22501 agatgttcaa aaagcgccta atggtcaatt tatgcacacc gtagaagtaa aagcaccaat
    22561 gagtgaagga caaacctatc atctattgat cgatcaatcg atgcgcaatc ggatcatcaa
    22621 aaaccataca gcaacccatc tactgcacaa ggcattgaaa gaagtacttg gcgagcatgc
    22681 aaatcaagct ggttctttag tcgctcctgg acatttacga ttcgacttta cccactttgg
    22741 tcaaatcact tcagatgaat tagctgaaat ggaaagaatc gtcaacgaaa aaatctggga
    22801 agctatccca gttgaaacaa ttgaaacaga tatcgataca gccaaaaaca tgggtgcaat
    22861 ggctttgttt ggtgaaaaat acggacatga cgttcgagta gtaaatattg gcggctggtc
    22921 aatcgaactg tgcggaggca cacatgtagc aaatacagaa aacatcggaa tcttcaaaat
    22981 cgtttctgaa tcaggaattg gtgcaggcgt gcgacgaatc gaggctgtaa caagtaaaga
    23041 agcgtacgaa ctacttgaag aagaagaaaa acaactaaaa caagtagcaa caattgttaa
    23101 atcacctcaa ttgaaagaag ttgtttcaaa agcggaacaa ttgcaacagc aagtaagaga
    23161 gctacaaaag gaaaatgaac aattagctag caagttagcg aatcaacaag ctggtgatgt
    23221 attcaaagat ccagaaacga tcaatggaaa aacaatcatt gcagcacaag taaaagtgaa
    23281 agatatgaat cagctgcgtc aattagctga ccaatggaaa caaaaaaata tttcagatat
    23341 tcttgtttta gcaaacgaac aagaaggaaa agtcagtctg ctagcggcaa tgacgaaaga
    23401 atcaaatgaa gctggactaa aagctggcga cttgattaaa gcaatcgcac cgaaagtagg
    23461 cggtggcggt ggtggacgtc cagacatggc acaagctggc gggaaaaatc ctgctggtat
    23521 tccagatgca cttaaagctg cgaaagaatg ggttgcgcaa caatagggcg tttacgtttg
    23581 acagaaaaac aaaagttcaa acattgaacg aaacatgatt atgggggcga agacaaacaa
    23641 gtcttgccct catacttttt tcaattggat ggcgcatcta atcaaatggt cggattcgga
    23701 tggtctttag ttgacgaaag tgacagaaat ttttacgatt agtataaata aaaaggggtg
    23761 aaaaaatgaa tagtagaaat aagacctttc gactagtctt acgtgcgatc ttgttagcaa
    23821 tcatcatcgt acaagcgatg gttccttggc tggggtttat tccattagga ttcattagtt
    23881 taacaattat ccatatcaca gtaattattg ctgcagtagt attaggccca aaagatggca
    23941 tggtgattgg cttgttttgg ggaatcgcta caattgttcg tgcctatgcc atgccaacaa
    24001 cgccatttga tacattagtg ttcacgaatc cgattatctc ggtagttccc agagtacttg
    24061 ttggcttagt agcaggcttg gttttccact ggatttatca aaaaaataaa tcaataacga
    24121 tcagctcagc ctttgcaggg gtactgggtt cattggtcaa tacagtctta gtgttaggat
    24181 tcatgggatt attttataca ggtgcaacgg cagatgccta tggtgtagac tctgctcttt
    24241 tatttaaaac cttagcaggg gttgcagcaa tcaatgggat accagaagca ataggtgcgg
    24301 gaatcataac accattacta gcaaaagcgt tatttgcagc aacaccgttg aaaccagaat
    24361 aatgaccatc ttttaatagt aataggacgc acgatagata gaaaaaggaa gagaggagag
    24421 gaaaacaatc gtcctctcca tctttttacc tgagcaacaa gcaaactaga aggggtaaaa
    24481 aacttggcta agaaacaaga agaaaaaaag aaacaaatac cgcaacagcg gacaaaaaaa
    24541 ggagtactag cgggtatctt aggattggtt acgaatctga tcttgtttgt ctctaagcta
    24601 atgatcgggc ttttttcagg aagtgtctcc attatggctg atgcagtgaa cagtctatca
    24661 gatactgctt catccatatt gactttgatc ggatttaaaa ttgctgcaaa gccagcagat
    24721 caggaacatc cttatggaca cgaacgcttc gaatatatca gtgggttatt tgtttcgatc
    24781 atcattactt atgtaggatt tcaattttta gagtcttctg ctgaaaaaat catccatcca
    24841 gaaaatgtct ctttaacacc catcgtcttt tttgtcctaa tcatttccat ctttttgaaa
    24901 tttgcacaag gcaaaatgta tcaggttatt gcgcaatcca ttgattctga aacattgaga
    24961 gctacagcaa cagatagtta taatgatgtc tttactacac tggctgtatt gctttcagca
    25021 gcaatcgaat gggtaactgg atggcgtatt gatggatata taggttttgc tttagcatta
    25081 tatatccttt atagtggatt gacgatgctt cgaaattttg tatacgaact attaggaagt
    25141 cgcccaacgc cagcagagat tgaagaaatg gaagagcatt taaatgcttg cgatgatata
    25201 cttggctatc atgatttatt ggtgcataac tatggaccca ataaacgctt tgcatcagtc
    25261 cacatcgaag tagatgaatc aatgaatttg aatgtggctc ataagattat tgatctgata
    25321 gaaaaagact ttaaaaagtc tttggatgtg gaacttgtct gtcacttgga tccaatgcct
    25381 gttgataacg aaaaatacta taatatcttg cggcagttaa agcaaattct tcttgaaaca
    25441 ggtacagggc ttaatattca tgattttaga gtaattcatc aaggaaaaac gctgcaattt
    25501 gatgtagtgg tccctgagaa attccagttt gttgatacag aactagaaag aatcttacgt
    25561 agtcaaatag aagaaaaaat cggagattac caattggaaa ttacgtttga tcacaattat
    25621 cttttatatt aaatgagaaa ctataaaaag actccttaaa gtttttaatt taaagagtct
    25681 ttttttcttt taattgttta tggagagcct gacgtgcaag attatctgct cctttatttt
    25741 tgttttcggg caaccattgc aggatcaata aagaaaattc ggatagaagt tcttggattc
    25801 tttgaaggta gggaaggaag agctcgttct tcgttgcgtt tttgtcgacc gtagaaacta
    25861 gtgttttgct gtctgaaaaa cagaaaatcg tttcattctg taagtcgttt ttctttagaa
    25921 attctaaagt ttttaaaaag acttcgaact ctgcttgatg gtttgtccct atagaaagcg
    25981 ggatagatag ctgttcttgt ttaccttgat agacaatcaa gattccacct ccactaggac
    26041 cagggttgcc ttttgtagag gcatcaatgt atacttttaa cataaaaaga gattctcctt
    26101 tttgttcaaa ctaatgttat ggtaagatta tatcattact gaatcggtga gaggttagag
    26161 aatgaaaaaa aatattcgat ggcagcctga actggcacat acgatcatct attggtcatt
    26221 gaccttgatc attctgtttt ttagtctgat tctttcttta gaaaacacag gtccttattg
    26281 gaaaagcaat tttgttttag gtatttttct tgtttttcta tttctaggtt tgcggcgttc
    26341 ttttatttta aaatcaaact ctgtgattat acgttatgct gctttttgga aagaccgaga
    26401 aatatctgtc ggacagattc gatctttgac gccaactaat actggggtgc aaatgatgtt
    26461 gcaagatgag aaagccccat ctgttttcat gatgcgaaaa aaagatcaag tagtcctttt
    26521 tgatcatttt gaaaaaaacg gacccaaatc agaatcaatt ccttatcctt atacgaaaaa
    26581 aagaaaaagc taattttaaa aatcagaacc taaaaaaacg aatttttaca ggaaaagaaa
    26641 agagtgagac gtacggttca tcccgtttag cgtctcactc tatttgttta tctattcatg
    26701 catactagta tcggtagtct gaacgttgcc tttcttgaca tgggcagctt gtgttcctcg
    26761 attcccttcg atgatctcaa attcgaccac ttgattttct tccaaagttt tgaatccctc
    26821 ttcttcaatc gcagtaaaat gtacaaaaat ttcttctgtg tcatcataac tgataaagcc
    26881 gtatcctttt ttgttgtcaa accattttac aattcctgtg gtcaaagcac tcacgacctt
    26941 tctaaagtct gatcatagcc ttcattataa agagtattta taagtacgtc aaataaatta
    27001 aaaacctgta attttatgtt ttttttctga ataaaaaaag gtataattgg catatcttaa
    27061 caaagaagat attttgttga ataaagttcg tgaaaagggg taatcaaatg aagaatgatc
    27121 tgcaaatttc acaagaaaca gagctagaac cgattgttga agtggctagt aaattagatc
    27181 ttaaggaaga cgatttggaa ctgtatggga aatataaggc gaaaatcaat ttttctggaa
    27241 tcaatcgatc aaaggataac gctgaggggc atttgatctt agttacttcc atcaatccaa
    27301 caccagctgg cgaaggaaag tcaacgatca ctgtagggtt aggagacgcg ctcaataaaa
    27361 tcaataaaaa atctgttatt gcattgcgag aaccctcact tggtcctgtg atgggaatca
    27421 aaggtggtgc tgcaggcgga ggatatgcac aggtattgcc tatggaagat atcaacttgc
    27481 attttactgg tgacatgcat gctatcacaa cagctaataa tgcattatct gctcttttag
    27541 ataatcatat tcatcaagga aatgaattga acatcgatgc ccgccgagtg atttggaaac
    27601 gggtagtcga tctcaatgat cgtgaactgc gtaaagtaat tgttggcttg ggtgggccaa
    27661 tccaaggtgt tccaagagaa gatgggtttg atatcacagt tgccagcgaa atcatggcta
    27721 tcttgtgtct tgcggcagac cttgaagatt taaaggctcg tcttgcaagg atcgttgtcg
    27781 gctatacatt tgaccgtcag cctgtgactg ctggagactt gaaagcagaa ggcgctctgg
    27841 cattactgct gaaagatgca atcaagccga atcttgtaca aacaatttac ggaacaccag
    27901 cttttgtcca cgggggaccg tttgccaata tcgctcatgg ctgcaatagt attttagcta
    27961 ccaagacagc attgagatta gcagattacg ttgtcacaga ggcaggattt ggtgcagatc
    28021 ttggcggaga gaaattttta gatatcaaag tacctaactt gaaaaaagcg ccggatgctg
    28081 tggtgattgt cgccactgtc cgtgcattga aaatgcatgg cggaatgaaa aaagatgaac
    28141 ttaaaaatga gaatcttgat gctttgaaag taggttttgc aaatttaaag cgccatatac
    28201 gtaatatgga acaatatcag ctacctgtga tcgtcgcaat caatgaattc gtaacagata
    28261 cagacaatga actcacactg ttggagcact tgtgtgaaaa tcaggggatt cttgccaaga
    28321 gagcgagtgt atgggcagat ggagcagaag gcggcgttga tttagcaaaa gctgtagtac
    28381 gattgattga tagaaaagaa gctaattaca agccattata ccgcctagaa gaaacgatac
    28441 aagaaaagac agaaacaatc gtcaagaaaa tttacggagg aaatggtgtt gttttcagca
    28501 aaaaagcgca aaaacaaata gaagagttta cgaagaatgg atgggacaaa ctgccgattt
    28561 gtatggcaaa aacacagtat tcattttctg atgatccttc actgctaggt gcaccggaag
    28621 atttcaccat cacgatccgc gaaatcatcc caaaattagg ggcaggattt ttagtagcct
    28681 tgactggaga tgttatgacg atgcctggtt tgcctaaaaa acctgctgca ttgaatatgg
    28741 acgtgacaaa tgacggagaa gtgttgggat tgttttaagc ttgattataa tctaccatcg
    28801 aaggagctgt attttgacta cagcttcttt ttttagacga tagggaatta attttgttta
    28861 cactaggatc attctttttc tttattgtat tttgagctat actaaagcat aataggaggg
    28921 tatgatgaaa acgacattta taagaaaaac cggtttttac ggtatgggta gctcgattca
    28981 gctaagaatc aacaatgaaa aacgcagtgt tctcagtcat gaacaaaaga taacgcttga
    29041 tctggaagtg ccctttacga tgcaggtcac cttttattgg ctaaaaagcc ccgtttatac
    29101 agtgaaagaa gcaaagcctg cttatgtgat cacaatgaat tacttgattc ttcaaatcta
    29161 tccgttttta tttctagcaa ctgggttggg ggccgttttc ctacaaaatt tatttttcag
    29221 tttttttgca gttattgtgt tgattgcgtt ctttttctat ataaaaaacc gggcatattt
    29281 gatcaaggag gaaaattatg gcaaactctg atcttttttt aatcagtttc aatcgaatcg
    29341 aaaaatggat gagagacgag atgggaaatg cacgaaatat gggatttacc gaacttgtca
    29401 gacgcctagc tcaaagaaaa caattaatga tcagaaaata cgaagatgat cttttacagc
    29461 ttgctcagtt aaggaatgcg atcgtccatg atcggatcgc agtcgatttt gtcattgcag
    29521 agccaaatga ttggacagtc aagcgaatcc agctgatcga acaagaactg attcagcctg
    29581 aaaccgtgct gcctcgattt gcaaaacgtg taacaggttt tgaacaagat cttccgttac
    29641 ttgaactgtt aaagatagta gcagaaaaaa ggtattcaca atttccgtta tacaataaag
    29701 gaaaatttgt agcattgatt actttacgta atatcgggtt ttggctagca aaagaaagcc
    29761 aaaaaggtcc ggttgattta acaaataaac gagcgttgga tttgatcatc caaaacggaa
    29821 agtacacaaa ttatcatttt gttccagcta aaacacatat ttacgaggtt gaagcgatgt
    29881 ttcgtgagca agggactttg gaagccgtac ttatcactaa agatgggaat cccgatggta
    29941 atctgctcgg gatcgtacga cctcgtgata tttataaaca aatagaaaag gattgatttt
    30001 ttgttaatta tttattggat attcagcgct ttgctagtag gagtggatca gttggtcaaa
    30061 tggtggatca ctgataatct agcgcttgga gagacgaaaa atcttatacc aggtattttg
    30121 tcactgaatc atattcgaaa cactggagcc gcatggagta tgctagaagg gaagatgtgg
    30181 ttctttacta tagtgacact cattgcagtc gttgtgattc tcacattgat gattaaaaat
    30241 cgtgaaaaag gtaatcgctg gttcatgatt ggattgagtt tgattctagc tggtgcgatt
    30301 ggcaatttta tcgaccgtat tcgtcttggc tatgtagtag atatgttcca gacagatttt
    30361 atgagctttc cgatttttaa cgtagcagat gtgacacttg tatgtggagt gatttgtatg
    30421 ttaatttata ttattttaga tgaaaaggat caaagaaaaa aatgacagaa cttaaaacga
    30481 cgattcacca tgaatctggt aggatcgata aagtattgac taatttattc accgattaca
    30541 gtcgttcgca aattcagctg tggctgaaaa atggagcggt ttctgtcaat ggacagccag
    30601 tcaaagcaaa ttacaaagta aaacaaggag acgagattac tgtcttgatc cccgaaccag
    30661 aaacattaga tgtggtccca gaaaatatcc cgttgacgat cgtttacgaa gatgaagatg
    30721 tagcggtgat aaacaaacct caaggaatgg tcgttcatcc ttcagcaggt catacgcatg
    30781 gcacaatggt caatgcgctg atgtaccata tcaaggatct ttctacgatc aacggcgtta
    30841 ttcgtccagg tattgttcac cgaatcgaca aagatacttc cggactgcta atggttgcca
    30901 aaaatgacca ggcacacgaa tcattagctc agcaattaaa agataagacc tctttgcgca
    30961 aatatgttgc tttagtccat ggagagattc ctcacgaaaa aggaaccatc aatgcgccaa
    31021 tcggccgttc gaaggaaaac cggaaaatgc aggcgataag agaagatggg aagtcggctg
    31081 tcacacattt tactgtattg gaacgtttcg aaggatttac tctggttgaa ttaaaactag
    31141 aaaccggaag aacacaccag atccgtgtgc atatgaagta tatcggttat ccgttagctg
    31201 gagatccagt atacggaccg aagaaaactt tgaaaggcaa tggtcagttt ctccatgcgc
    31261 aaatgttagg ttttacgcac ccgactacaa acgaaaagat gatctttgaa gcacctcttc
    31321 cagaagtttt tgaaaaaact ttagaaaaat taagaaaaaa cattgcattt taatttagat
    31381 aacggtatag taattaacaa cgaaagcata aaacttaata gaattccttt aagtttagtc
    31441 ccgtgaggct aagaagggtg ggagcaagga tacgtatggt gcgccataag tagattgctc
    31501 attgacatag tatagctatc agtgtatcag tatacgctca gtctaaacat tcctcctgtc
    31561 ctcttagggc aggagttttt ttatgcaaaa aagtaggggg aatgaaaatg caagcaaaag
    31621 aagtagtaga tcaggtaacc atgaagcgag cattgactcg aattacttat gagattatcg
    31681 agagaaacca tagtatccaa gatatcgtat tggtaggtat caaaacacga ggaatctaca
    31741 ttgcatcaag aattgccgaa cgcctgaagc agctagaaga tatcgatatt cctgtaggag
    31801 aactggatat cactttatac cgagatgaca aaaaagaaaa tccagaagaa ccggaacttc
    31861 attcttctga catcccagtt tccttagaag gaaaagaagt tattttgatt gatgatgttt
    31921 tgtatacagg acgaacgatt cgagcagcaa tggacgcagt aatggatttt ggcagaccaa
    31981 gaaaaatttc attagcagtc ctggtcgatc gtgggcatcg agaattgcca atccgtgcag
    32041 attatgttgg gaagaacatt ccaacatctc gggcggaaga gattttagta gagatgcaag
    32101 agcttgatgg acaagatcgc atcatgattt tgaaagagga agattagagg agagagaaac
    32161 atgtcggaga aaaaaggatt tcataatgat gacgtggtgt tagatatcca cgatcgccca
    32221 accccaggac attgggtagg attaagttta caacacttgt tcacgatgtt cggagcaacc
    32281 gtgttagtcc ccatcttagt aggtatcgat ccaggtattg cgctagtcag ttcaggacta
    32341 ggtacattgg tgtacttatt tactactaaa ggaaagatac ctgcgtactt aggaagcagc
    32401 ttcgctttca ttgcagcgat gcagcttttg atgaaaacag atggttatcc agcgattgca
    32461 caaggagcag tcacaactgg attagtctat ttgattgttg cgctgatcat caaaaagatc
    32521 ggttcagcat ggctggataa aatcttgcct ccaatcgtcg tcggtccagt catcatggtt
    32581 atcgggttgg gacttgcggc aaatgcagca acaaatgcta tgtataatca gggcacgtat
    32641 gatttcaagt atattgctgt agcgctgatc acattaggat taacaatttt cttcaacatg
    32701 tggctaagag gttttctcgg attgattccg attttactag gtattattag cggatacatc
    32761 gtagccttgc tgtttggaat cgtggatact aagccaatcg tagacgctgc ctggtttgct
    32821 gttcctaact ttgagattcc ctttgtacaa tatgaaccaa agctatatct tggagcaatc
    32881 acaacaatgg cacccatcgc ttttgtgacg atgacagagc atatcggcca tcttatggta
    32941 ttgaataaac taactaaacg taatttcttt gaagaaccgg gtcttcataa gacgttgaca
    33001 ggtgatggat tagcacaaat cgtggcagcc tttgtcggag gtcctcctgt aacaagttac
    33061 ggtgaaaaca ttggtgttct ggctatcaca cgtgtccata gcgtgtttgt gatcggtgga
    33121 gctgcagtat ttgcagtcat tttaggattc gtaggaaaat taagcgcttt gattttaagt
    33181 atccctggac cagtcatctc aggaatcagt tttatcttat ttggagtaat tgccgcaagt
    33241 ggaatgaaga ttttaatcga gaacaaaatc gattttgata aaaagaaaaa tctgttgatt
    33301 gcttcagtta tcttagtagt aggaattggc ggactcgtct ttgaagtagg cacatttaca
    33361 ttgtcggcaa tggcattagc aactgtactt ggcatcattt taaatcttat cttaccagaa
    33421 acttcacgaa gcgaagaaaa ctaaggagga aacattatga tcatcaaatc agaacgtatc
    33481 agcttaaagc atttattgac cgttgaggca cttactgatc aagaagttat gggcttgatc
    33541 cgcagagggc aggaattcaa gcaaggtgct aattggactc cgcaaaagcg ccagtacttt
    33601 gctacaaacc tctttttcga aaatagtact cgcacacata aaagcttcga tgtagcggag
    33661 aaaaaactag ggctagaagt gattgaattt gaagcaagca caagctctgt ccaaaaaggg
    33721 gaaacactat acgatacagt actgacgatg tcagcattag gcgtagacgt agcggttatc
    33781 cgtcatggtg atgagaacta ttatgacgaa ctgatacaaa gtaaaacaat ccagtgttcg
    33841 attatcaatg gaggagacgg aagcggacag catccaaccc aatgcttgct tgatttgatg
    33901 accatttatg aagaatttgg tcattttgaa ggtctgaaag ttgccatcgt aggtgatatc
    33961 actcactctc gcgtagctaa atccaatatg cagatgttaa aacgtctagg cgcacaagta
    34021 ttcttctcag gtcctagaga atggtacgac gatgaattcg aagtgtacgg acattatatg
    34081 ccattggatg aactactaga tcaagttgat gtcatgatga tgcttcgggt gcaacacgaa
    34141 cgccatgatg gaaaagaaag tttctcaaaa gaaggctacc atcaggaaca tgggttaaca
    34201 gttgagcgtg cgaagaagtt gcaaaaacat gcaatcatta tgcacccagc accagttaac
    34261 cgtgatgtag aattagctga ttcattagtg gaaggattac agtcgcggat cgttcagcaa
    34321 atgagcaatg gagtattcgt cagaatggca atcttagaag cagtgctaaa tggaaaagcg
    34381 taattatagg aggatgaaaa aatgaaaaca ctcattaaaa acggtcagat caatacacga
    34441 aaaaatatga cgacaccagc agaaatttgg atagaagatg ggcgaatcaa agcaatcggt
    34501 acaggattct cagaagccga atttgatgaa gtgtttgatg caaagggaca attgatcaca
    34561 cctggtttag tggacgtcca tgttcatttg agagagccag gatttacata caaggagacg
    34621 atcgaagctg ggactcgctc agctgctcgt ggcggtttca ctactgtatg cgcgatgccg
    34681 aatttaaatc ctgtacctga tacggcagaa aagctgagac aagtatatga catcattaga
    34741 aaagatgcag ttgtgaaggt attgcagtat gcgccaatca cagaaaatct gcgaagtgag
    34801 aaattagttg atcaagaagc actgattgaa gaaggtgctt ttgctttcac aaatgacggt
    34861 gtaggtgtcc aaacagctgg gacgatgtat ctggctatga aagaagctgc aaaaaacaat
    34921 aaagccctag tagcccatac agaagatgaa tctttattat ttggcggcgt tatgcacgct
    34981 ggcaagaagg cagaagaact tggtcttccc gggatcttaa gcgtaaccga gtcttctcaa
    35041 attgctagag atctgttgct agctgaagca actggtgttc attaccatgt ttgccatgtt
    35101 tctactaaag aaagtgtccg tgtaatcaga gacgcaaaaa aagcaggaat ccacgtaaca
    35161 gcagaagttt caccccatca tcttattttg atcgacgaag atattcccga agatttcggc
    35221 ttttggaaaa tgaacccgcc gctaagagga cgggaggaca gagaagcttt gatcgaaggc
    35281 ttgcttgacg gtacgatcga ttgcatcgct acagaccatg ccccccatgg attagaagaa
    35341 aaaagccaaa gtttcatgaa atcaccattt ggtatcgtag gaagcgaaac agcgtttcaa
    35401 ctgatctata ctcattttgt agaaactggt cgattcacgt tggaacaagt gatcaattgg
    35461 ttggcagtta aaccggctga gatttttggt ttgaatgcag gaacgctaac ggttggtgcc
    35521 cccgctgatg tagcagtttt tgatattaca caaacttgca cgatcgataa agaagacttt
    35581 ttatcaaaag gggagaatac accttttatc ggctggaaag taaaaggcga aacacaaatg
    35641 acttttgtca acggaaaact tgtgtggcag aagggagaat aaaagtatgg aacgtttgct
    35701 tatattagaa gatggaacag ttttcgaagg aaaggctttt ggtgcagatg ctagtgtctt
    35761 cggagaaatc gtttttacaa caagtatgac tggttatcaa gaaactatca ctgatcaaag
    35821 ttttaacggt caaatcatta catttaccta tcctatggtg ggtaattatg gggtaaatag
    35881 agatgattat gaatcgattt ctccaacatg caaaggagta gtagtaaaag aacatgcacg
    35941 tgtagcttca aactggcgaa accaaatgac gctagattca tttttgaaac aaaaaggaat
    36001 tcctggaata tcaggtatcg atacgcgcgc ccttactcgc aaaatccgtg aagtcggaac
    36061 gatgaaagct agtatcgttg atgctggaga ttccttcgaa catgcatttg atcagctgaa
    36121 agcaaccgtt ctagcaacca atcaagtgca gcaagtctca actaacaaac cttatccaag
    36181 tccaggaaca ggtcggaaag tcattgtcat tgacttcggc ttgaaacata gtattttacg
    36241 agaattgagt aaaagaaact gtaacctcac agtacttccc tataacacag atgccgagac
    36301 gattttagcg ctatcaccag acggagtcat gctgacgaat ggacctggtg atccaaaaga
    36361 tgtgccagaa gcaatcgaaa tgatcaaaaa aatccaagga aaagtaccga tttttggtat
    36421 ttgtctcggt caccagttat ttgctttagc caacggtgca gatacctata aaatgaaatt
    36481 cggacatcga ggattaaatc acccggtacg agaagtagcg accggaagaa ttgattttac
    36541 ttctcaaaat catggctatg cagtcgatga agccacgatt gatccagaaa aactcatcgt
    36601 aactcatgta gaagtaaatg acggaacagt agaaggagtc aaacacagag attatcccgc
    36661 tttcagtgtg cagtttcatc cagatgctgc ccctggtccg catgatgcac tacatttatt
    36721 tgatgaattt atggaaatga tggacgcagg aaaggagcag taaacagatg ccgaaacgta
    36781 cagatatcaa gaaaattatg gtgattggat ctggtccgat catcattggt caagccgcag
    36841 aatttgacta cgcaggaaca caagcctgcc ttgccttaaa agaagaagga tatgaagtgg
    36901 ttttagtaaa ttctaatcct gcgacgatca tgacggataa agaagtggca gatcaagtat
    36961 atatcgaacc gatcacactt gaatttgtat caaggattct tcgaaaagag cgtccagatg
    37021 ctctccttcc aactttaggc ggacagacag gcctcaatat ggcaatggag ctggcagcat
    37081 caggtatttt agaagaattg aacatagaac ttctaggaac aaagctttct gcgatcgatc
    37141 aagcagaaga tcgtgatcta ttcaaacagt tgatgaaaga attgaatcag ccaatccccg
    37201 agtctgaaat cgtcaataca gtagaagaag ctgtagattt tgcaaataaa atcggctatc
    37261 ctgtcatcgt tcgtccagcc tttactttag gcggaactgg tggaggtatg tgtgaaaatg
    37321 aagaagaatt acgtatcgta tcagaaaatg gattaagtct ttcccccgct acccaatgtt
    37381 tgattgaacg aagtattgca ggttttaaag aaattgaata tgaagtgatg agagattcgg
    37441 ctgataatgc aatcgttgtc tgcaatatgg aaaattttga cccggtgggt atccatacag
    37501 gtgattctat cgtatttgcc cctagtcaaa cattgtctga ccatgaatat caaatgcttc
    37561 gtgatgcttc tctttcgatt attcgagcac ttaagatcga gggaggctgt aatgtccaac
    37621 tcgctcttga tccacatagt tttaattatt atgtgattga agtcaatccg cgtgtttctc
    37681 gttcatctgc gttagcaagt aaggcgactg gctatccgat cgctaagcta gctgctaaaa
    37741 tagcgcttgg attaacatta gacgaaatga aaaatcctgt tacaggaacg acttttgcag
    37801 aatttgaacc tgcattagat tatgttgtag ctaaaattcc tcgctggccg tttgataagt
    37861 ttgaaaacgg agaacggctt ttaggcacgc agatgaaagc aactggtgaa gtgatggcta
    37921 tcggtcggaa tatcgaagag tctttattga aagctgtgcg ttctttagaa atcggtacac
    37981 accatatcga acttcctgag ttggcagaca tcacggacga tgacctaatc caaaaagtgg
    38041 ttcgagcaca agatgatcgc ctgttttatg tagctgaagc tattagaaga gggtacacca
    38101 tagaagaatt agcggatttg acaaaaatcg atttattctt tttagataaa ctgttgcata
    38161 tcgtagaaat cgaacagcag ttgaaacagc agccgttgga tgacaagata ttgaaaacag
    38221 cgaaacaaaa cggttttaca gacaaaaaag tggcggagct gtgggaaata aacgaagaag
    38281 ccgttcgcca gatgagaaaa gaaaatgatt tgattcctgt atataagatg gttgacactt
    38341 gtgcagcgga attcgaatca aaaacacctt atttttatag ttcctatgaa tatgaaaatg
    38401 aaagttcacg atcagaaaag ccctcaattc tagtactggg atctggtcct atccgtatcg
    38461 gacaaggggt tgaatttgac tatgcgactg tgcattctgt caaagcaatc cagcaggcag
    38521 gatacgaagc aatcatcatg aacagcaatc ctgaaacagt ttcgactgac ttttctattt
    38581 cggataaatt atattttgag ccattgacct tcgaagatgt gatgaatgtg atcgagctag
    38641 aacagccgat aggagtaatc gttcaatttg gtggtcagac agcaatcaac ttagccgaac
    38701 ctttgactgc tgctggtgta aaaatcttag ggacaacgat cgaagacttg gatcgtgcgg
    38761 aaaatcgtga tttattcgag caagcgctga aagagttgga tattcctcaa cctttagggg
    38821 atactgctac aagtaaagaa gaagccgtgg ttatcgcttc gagaattggt tatccagttc
    38881 ttgttcgtcc aagctatgta ttaggcggac gtgcgatgga aatcgttgaa aaccagcgag
    38941 atttagaaga ttacatggaa catgctgtga aagcatctcc agaacatcca gtactagtgg
    39001 accgttactt gcttggtggt gaatgcgaag tggatgcgat ttgtgacggt gaaacagtac
    39061 tcatcccagg gatcatggag catattgagc gtgctggtgt ccattcaggg gattcaatgg
    39121 ctgtgtatcc acctcaaaca ttatctgaat cgatcaagaa aacgatcgaa gattacacga
    39181 tccgcttagc attaggtttg aattgtgtcg ggatgatgaa tatccagttt gtcatccatg
    39241 aaaatgaggt ttatgtgatc gaagtcaatc ctcgagcaag tcggactgtt ccattcttaa
    39301 gtaaggtgac aggaattcca atggcacaaa ttgcgacgaa agctattttg ggagagaaac
    39361 taaagacact tggttatgaa aacggacttt atccagaaac aaaagaagtt catgttaaag
    39421 cacctgtctt ttcttttacg aaattgcaga aagttgatac ttacttaggg cctgaaatga
    39481 aatcgactgg agaagtaatg ggatctgaca gacaattaga caaagctttg tacaaggcat
    39541 tcgaagcttc tggattacac ttgcctgaat atggagccgt attatttacg attgcggacg
    39601 aaacaaaaga agaagcacta gaattagcta aacgttttac agaaattggc tatagtctga
    39661 taggaacgaa acatacagct gcttatttcg aaaaaaatgg tctggtcgtt gaaacagtag
    39721 cgaaaatttc tgaggaagca gcagaaaaaa atgtagtcga cttaataaga gatggtaaaa
    39781 cacaagtggt tgtcaatacg attgataaag atcggggaaa tgcttctaaa gacggtttca
    39841 tcatccgaag agaagcagtt gaacatggta ttccattgtt tacttcttta gacacagcag
    39901 atgcgatcgt tcgagtgatg gaatcccgtg ctttttcaac taaccctatt taacggcgag
    39961 cacatgttaa gacaaaaaga cgaaaaaccg ttgcaggagg ggacagatga aacaagaaat
    40021 gatggaaatc gttcagcaaa aacagctggc accaaagatt tttgaaatga cattgaaagg
    40081 tgatctagta aaagaaatgc agcaaccagg acagttcttg catattctcg tgccgcgtgc
    40141 agacttgcta ttacgccgac cgattagctt gaatcatatc gaccacgaaa caaatacttg
    40201 ccgtatcatt tatcgggtgg aaggtgaggg gactagagtc ttctctactc tttctgccgg
    40261 agaccattta gatgtcatgg gtcctttagg caatggtttt gatttgtcca atcttcatac
    40321 aggagacgaa gtatttataa tcggtggagg aatcggtgtc cctcctctat atgagttatc
    40381 aaaacagtta gtcgaaaaag gaattaagcc aacgcatttt ttaggttttg ccacacatga
    40441 agtcgtttat tatgaaaaag aatttaatcg tctaggagaa acaagaatcg ctactgacga
    40501 tggaacatac ggtatgcacg gaaatgtcgg taatttattg ttaggaacga agacggaacc
    40561 tacagctgtt tttgcatgtg gttcaaacgg gttattgaag acagtggaac agctgttttc
    40621 ttcgcatcaa aatgtccagt tatcattaga atcccgcatg gcatgtggga taggggcttg
    40681 ctatgcttgc gtttgtcata cgccggaaga tgaaacagga acaaaaagtg tgaaagtttg
    40741 tgacgaagga ccgattttta aaataggaga ggtaattata tgaatgagaa tcctttagcg
    40801 gtcaaacttc ctggcctaga attaaaaaat cctatcatgc cggcaagcgg ctgtttcggt
    40861 tttggcaaag aatacggaaa atactatgat ttgaatcagc ttggatcgat catggtcaag
    40921 gcaacgactc caaatgcccg ctttggcaat cccacgccaa gagtggcgga aacaccatct
    40981 ggcatgttga atgcaattgg tttgcaaaat cctggtctag acgttgttat gcataaactt
    41041 cttccagaat tagaggagta caatgagtta cccatcatcg caaatgttgc tggtgcttgt
    41101 gaagaagact atgtagaagt ttgtagcaaa attggcgaag caccaaatgt gaaagcaatc
    41161 gagctgaaca tttcttgccc aaatgtcaaa catggcggaa ttgcttttgg aacagattct
    41221 gatgttgctt ttcaattaac acaagcagta aagaaagttt cttctgttcc tgtctatgtc
    41281 aaattgtccc caaatgtaac agacatcgtt ccaatcgctc aggcaatcga agctggaggg
    41341 gcagatgggt ttacgatgat caatactctt cttggtatgc ggattgacct taagacaaga
    41401 aaaccgattt tagccaatca aacaggtgga ctctccggac cagcaatcaa gcctgtcgct
    41461 attcgtttga ttcaccaagt agcaagtata tccagccttc caatcattgg gatgggcggc
    41521 gtgcagacag tggatgatgt tcttgagatg tttatggctg gagcaagtgc tgtagctata
    41581 gggaccgcga atttcaccga tccgtatatc tgtcctaaac taattaaaga gctccctgtg
    41641 cgaatgagcg aactaggcat cgaatcgctg gaaagattaa tcaaagaagt gagagaggaa
    41701 cgtgaacgat gaatcaacta gaccaacgac caattattgc attagatttt tcaactaggc
    41761 aagaggtaga agactttctt cgatttttcc cgaaagaaga aaaattgttt gtgaaaatcg
    41821 gaatggaatt gttctaccaa gaaggacctg aaatcgtacg ttatctaaaa gaggctggtc
    41881 ataatgtatt tttagatctg aaacttcacg atattccgaa tacggtcgaa aaagcaatgc
    41941 ggggattggc aaagcttggt gttgatctca cctgtgttca tgccgctggc ggtattcgta
    42001 tgatggaggc ggcaatgcga ggattagaag aggggactcc agaaggtgga aaacgtccgc
    42061 tacttctagc tatcactcag cttacttcta caagtgaaga agaaatgcat gccgatcaac
    42121 taatcgaagt ttctttagaa aaaagtgtga tccattatgc tagctgtgcc aaaaaagcag
    42181 gattagatgg tgtggtttct tctgcttggg aagtagaagc gatcaaagaa atggcaggag
    42241 acgaatttgt ttgcttaact ccgggtataa gaccagaagg aacagtcgca ggagatcaga
    42301 caagagtagt cacaccttct caggcaaaaa agatcggctc aacttttatc gttataggtc
    42361 gaccaatcac tcagtcaact aatccttata aagcatatca aacgattcaa acagaatgga
    42421 gctaacctaa aatgaatgtt gaacaaagta tcgcaaaaga cttattagaa attgaagcag
    42481 tatttttaaa tccaagtaat ccttttacat gggcaagtgg aatcaaaagc ccaatttact
    42541 gtgataaccg aatcacgatg agctatccaa aagtccgaaa agagatcgca aaaggattag
    42601 caagcaaaat caaagaagct taccctgaag tacaagtgat tgctggaact gcaacagcag
    42661 gaatccctca tgccgcatgg gtagcggaaa tactggattt gccaatggtc tatattcgaa
    42721 gtaaggcgaa agatcatggg aaaggtaacc aaatcgaagg acgtatattt gaaggtcaga
    42781 agatggttgt gatcgaggat ttgatttcta caggcggaag tgtcttggaa gcagctgaag
    42841 cagcaaaaag agaaggtgcg gatatccttg gtgttgcggc tatctttacg tatgagttgc
    42901 caaaaggaaa agcaaatttc gaaaaagccg aaatccctct tatgacgttg accaattatt
    42961 ctgtattgat tgaagcagct ttagaagatc gttatatcga tgagcaagaa ttaactctgc
    43021 taaaagaatg gaaaaaagat cctgaaaatt ggcagattta agcgcgcttg atgaagatag
    43081 agaaatccga tttgtgtctc cagaatcatc aattggaata gcaaaaatcc aaaaactgat
    43141 tgcgagaagc atcttttcat caggtatgaa aagaaacttc tatcgtgatc agtttttgat
    43201 ttttttaaaa ttcatttttc taagactggt tagcagaaag aagagaataa tctcttctct
    43261 tttttctatt atatatagat tttctctatt ttttggtaag ggagctgttt caaaaatggt
    43321 aaagagcgga aatgttgcga atatcagtta tttaatgata tgatattcgg aaaaagaaat
    43381 ctcaaaggga ggtaactttt atggggttga atgaatttat tttaggattg aacaatttag
    43441 agacgatcac aagggcacct ggatttttta aatatacaga gcatacagtt gcagcacatt
    43501 cgtatcgtgt agcatctatt gcgcaagttc taggagacat tgaagaagcg aatggacaac
    43561 cgatcgattg gaaactgctc tatgaaaaat cattgaatca tgactacaca gagcgtttta
    43621 ttggtgatat caaaacgccg gtgaaatacg caagcaaaga gttaagaggg atgctgcaaa
    43681 cagttgaaga aaagatgacg gacgaattca tcaaccaaga gattccagct gagttccaag
    43741 aagtttatcg ccgtcggttg ttcgaaggaa aagatgaaac cgtagaagga gaactattag
    43801 ccatttcaga taaggttgac cttctttatg aatcttttga tgaaatcatc aaaaataacc
    43861 cggaaaaagt ttataaggag atgttcattg aggcagtgac tacgattaaa agctttgaac
    43921 atcggccttc tgttcagtat ttctttaaag agatatttcc ggaactattg aaccaggaat
    43981 tttatggaaa agaggcgttt ttagaaaccg tctcaacgat tgtaaagaaa gaagaaaaat
    44041 aaaaatgata tgagatgcag ggggaagtat agttgaaaaa gaatatggat gtcgaatggg
    44101 gctattctgg agacaaaggt ccggaaaatt gggcgagttt atgtgattgg tttgcgagag
    44161 gtgcggaatt tccattgcag tcgcctattc atttgaccaa gggggaagaa accaaaatag
    44221 aaggagacac gctttccttt cattatcaaa agcaacggtt tactgacaag gaattcaaaa
    44281 acacgattca tttagtacct tttgaccaac tcagttatgt agagttcaag aaagaacgct
    44341 attatttgac agatattcat ttccacttgc cttctgaaca tatcatcaac ggaaaacagg
    44401 aagatattga atttcatttt gttcatatga acagcaaaaa agagaatcta gttgtgggtg
    44461 tcatgtacca gctaatggta caggaaggtt ggctgtataa tcaagaaaat ggggaaagtt
    44521 ggaacttaga aaaacatgag cattgggtaa atccggatgt gttctttccg gagcaaaaaa
    44581 gccatttcca ttatattggc tcattgacaa ctccgcctac cagcgggcca atccaatggt
    44641 ttgtaatgga tcatgtagga aaactgaacc aagagtttct tctccccttc gatggtcagt
    44701 atgctcgacc gaacaaccga ccaatccagc ccttgaatgg aagagaaatt tattatttcg
    44761 aggagtttga gcaatgaata ttcagcagat gaaatacgtg gtcgctgtag caaacaatgg
    44821 tagtttcaga gaagcggcaa agaaaatgtt tgtctcccag ccaagtcttt cagctggtat
    44881 caaagaatta gaaaccgagt tagggatcac gctgtttact cgaacgaatc gaggggcata
    44941 tttaacagaa gaagggcaag acttcttaaa tcgtgccgag aaaatattgg tccagcttga
    45001 tacattagaa actcactatc taacgagtga aaagagagac aatttttcga tcgctgccca
    45061 gcattatgat ttcttaggtc ctttgaccgc acagctactc gaggaattca aagacgagat
    45121 caaacaattt cggatcatgg agacgacaac agcgagagtg atcgaagaag tcaaagagca
    45181 gcacagtgag atcggcatta tttatatgaa tgagcataat caagctggta tcagaagata
    45241 tttacaacaa ggagagctaa gtggccgcag cattggaaaa ttcaaaacgc atattttttt
    45301 aaggcaagga catccattag ctgaacacgc agagatcacc aaagaagaat tgttatctta
    45361 tcctcaagtt cgttttacac aggatggaaa taatttcccg tatttttatg aagaccttat
    45421 cgagatccct gatcaagaaa cagtgattta tacgagtgat cgaggtactt tgatgaatat
    45481 cgttcttgaa actgatgctt acgcttcagg atctggtatt gtgattggag ggattcgcga
    45541 gcacttgcgg ttaatccctt tggcagaagg cgaactaaat gaattttata tcatttattc
    45601 ggcgaaacgt ccgctcagtg aaatcgctca aagatttatc aaagagctta ctcggttact
    45661 ggaccagcag agctaagaaa agcagatgag aacgctgttt attgaacacc gttgatcaaa
    45721 aaagaggggt tgtgacagaa ttttgtcaca actcctcttt ttgtagcaaa caatttataa
    45781 ctaattgctg ttgtgggaaa gtacgcaaat atgattctag aaacactcat tgattacctg
    45841 ttttgtttca tagctgtaat tttttcttct tctggtgtca cgataatcgt cttttggttt
    45901 tcataaataa cataaccagg cttggcgcca tttggttttc ggatatgctt tacctgtaca
    45961 agatcaaccg gcacttgggc agagtatcgg tattttgaaa aataggcagc taactcggca
    46021 gcttctgtaa ttgtttcatc tgagggcttg tcacttttaa tgatgacgtg cgaaccgggt
    46081 atattttttg catgaagcca atagtcggtt tttttagctg tcttcattgt cagctggtca
    46141 ttttgaagat tgtttttacc aacaagaatg agcgttccgt cagaagaaaa gtattgatca
    46201 ggctgagatg gtttcttacg tttttgtttt tttaagttct tctttttcag gtatccttct
    46261 gaagtcaatt cttctttaat tgcttcgata tccattgggc cggcaatttc cagttgagat
    46321 aagacagatt caagatattg gatttcatct ttggcttctt ggatttgttc gccaatcagc
    46381 ttaacagcgt tcttcagctt ttggtatcga tggaaatatt tctgtgcatt ttgatttggt
    46441 gtcaatgccg gatctagtgc tattttgatt ggccgatctt cttcataata attaggtaaa
    46501 acaacttctt ttgcgccgcg tggtacttgt gtcagaaaag tagtaagtag ttcgccattt
    46561 ctacggtaat tctcagcgtt ttcggattct ttgagtgttt gttcacgttt tttcaattta
    46621 tttttatttc gttttagttc attttcgatt tttcgaatca gttctccgcc ttgttgtttt
    46681 gcccgatctt tctctgcttt ttcgtgataa tagctatcta ataattgact gagtgttgga
    46741 taagctgtta cagcagatgc ctgttcggac aatacctgat aagcaatcgg tgtgaaaaaa
    46801 tctttatttt ccgtttcgta aaaagtagga ataggctgat ggctgatggc tgagaaaaat
    46861 tcgtcccata caaccatctt ttcattagga cgttccagta atcttttggt cagctcttga
    46921 gcgatatcga aaccgattcc ctgaaactga cgttggattg cttttggatc tagttcagtt
    46981 tgggaaagcc gctgaaagat tttttctttc tcggagctaa atgggttcaa gacctcttgt
    47041 tttggcggag caacatattc tacaccagga agcaaagaac gataagtgtt ttgagagctt
    47101 ccaatgtgtt tgatcgcatc cagtattttt ccagtttctc gattaactaa gacgatcgta
    47161 ctatgacgtc ccattaattc aacgatcaat atgatatttt gtagatcacc caactcgttt
    47221 cgtttagcaa agtggaaatg gatcactcga tcattttcga tttgttcgat gtcttctaaa
    47281 atagcgccat ccaaatattt tcgaagcatc atcacgaagt taggcggatt atcaggattc
    47341 tgataatcga tttgggtaat ctgtacacga gcataacttg ggtgcgcaga taataataat
    47401 cgatggtttt tgccgcgtga acgaatcacc agcacgattt cattttcata aggctgatgg
    47461 attttcgaga tccggccaga tagtaacgtt tcacgcaatt cgttgaccat cgcgtgggta
    47521 aatacaccat caaaggacat ggttcattcc tcatttcttt tcataagtat attcattata
    47581 acgaaaaaaa gaagaaaaac caatttggcc gaatagtaaa gcaaaagtct agaaaaatct
    47641 tggactaatt acataaaaaa cagtgtttct atcgttgaaa tccaaatttt taggt
//
LOCUS       DAMEET010000006        46015 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.006, whole genome shotgun sequence.
ACCESSION   DAMEET010000006 DAMEET010000000
VERSION     DAMEET010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 46015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.006"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(95..493)
                     /locus_tag="PB780_000325"
     CDS             complement(95..493)
                     /locus_tag="PB780_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HDE0732401.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(490..1263)
                     /locus_tag="PB780_000326"
     CDS             complement(490..1263)
                     /locus_tag="PB780_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HDE0732402.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1260..1943)
                     /locus_tag="PB780_000327"
     CDS             complement(1260..1943)
                     /locus_tag="PB780_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HDE0732403.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2110..2997)
                     /locus_tag="PB780_000328"
     CDS             complement(2110..2997)
                     /locus_tag="PB780_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDE0732404.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(2994..3863)
                     /locus_tag="PB780_000329"
     CDS             complement(2994..3863)
                     /locus_tag="PB780_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDE0732405.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4000..5286)
                     /locus_tag="PB780_000330"
     CDS             complement(4000..5286)
                     /locus_tag="PB780_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDE0732406.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5559..6137
                     /locus_tag="PB780_000331"
     CDS             5559..6137
                     /locus_tag="PB780_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDE0732407.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6137..7066
                     /locus_tag="PB780_000332"
     CDS             6137..7066
                     /locus_tag="PB780_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDE0732408.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7063..8799
                     /locus_tag="PB780_000333"
     CDS             7063..8799
                     /locus_tag="PB780_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDE0732409.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8862..9698)
                     /gene="pheA"
                     /locus_tag="PB780_000334"
     CDS             complement(8862..9698)
                     /gene="pheA"
                     /locus_tag="PB780_000334"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HDE0732410.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9695..10204)
                     /locus_tag="PB780_000335"
     CDS             complement(9695..10204)
                     /locus_tag="PB780_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDE0732411.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10197..11492)
                     /gene="aroA"
                     /locus_tag="PB780_000336"
     CDS             complement(10197..11492)
                     /gene="aroA"
                     /locus_tag="PB780_000336"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDE0732412.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMSADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11505..12596)
                     /locus_tag="PB780_000337"
     CDS             complement(11505..12596)
                     /locus_tag="PB780_000337"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HDE0732413.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12613..13779)
                     /gene="aroC"
                     /locus_tag="PB780_000338"
     CDS             complement(12613..13779)
                     /gene="aroC"
                     /locus_tag="PB780_000338"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDE0732414.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13781..14845)
                     /gene="aroB"
                     /locus_tag="PB780_000339"
     CDS             complement(13781..14845)
                     /gene="aroB"
                     /locus_tag="PB780_000339"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDE0732415.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14878..15900)
                     /gene="aroF"
                     /locus_tag="PB780_000340"
     CDS             complement(14878..15900)
                     /gene="aroF"
                     /locus_tag="PB780_000340"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDE0732416.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16559..17533)
                     /locus_tag="PB780_000341"
     CDS             complement(16559..17533)
                     /locus_tag="PB780_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HDE0732417.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17514..19397)
                     /locus_tag="PB780_000342"
     CDS             complement(17514..19397)
                     /locus_tag="PB780_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HDE0732418.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDIK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19415..21640)
                     /locus_tag="PB780_000343"
     CDS             complement(19415..21640)
                     /locus_tag="PB780_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDE0732419.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21755..22789
                     /locus_tag="PB780_000344"
     CDS             21755..22789
                     /locus_tag="PB780_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDE0732420.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22856..23869)
                     /locus_tag="PB780_000345"
     CDS             complement(22856..23869)
                     /locus_tag="PB780_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDE0732421.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(23947..24135)
                     /locus_tag="PB780_000346"
     CDS             complement(23947..24135)
                     /locus_tag="PB780_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDE0732422.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(24575..24703)
                     /locus_tag="PB780_000347"
     CDS             complement(24575..24703)
                     /locus_tag="PB780_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDE0732423.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(24706..26079)
                     /gene="rlmD"
                     /locus_tag="PB780_000348"
     CDS             complement(24706..26079)
                     /gene="rlmD"
                     /locus_tag="PB780_000348"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDE0732424.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(26190..27245)
                     /locus_tag="PB780_000349"
     CDS             complement(26190..27245)
                     /locus_tag="PB780_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDE0732425.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(27255..28685)
                     /gene="gatB"
                     /locus_tag="PB780_000350"
     CDS             complement(27255..28685)
                     /gene="gatB"
                     /locus_tag="PB780_000350"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HDE0732426.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(28682..30154)
                     /gene="gatA"
                     /locus_tag="PB780_000351"
     CDS             complement(28682..30154)
                     /gene="gatA"
                     /locus_tag="PB780_000351"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HDE0732427.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(30154..30459)
                     /gene="gatC"
                     /locus_tag="PB780_000352"
     CDS             complement(30154..30459)
                     /gene="gatC"
                     /locus_tag="PB780_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HDE0732428.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(30565..32601)
                     /gene="ligA"
                     /locus_tag="PB780_000353"
     CDS             complement(30565..32601)
                     /gene="ligA"
                     /locus_tag="PB780_000353"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDE0732429.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(32618..34852)
                     /gene="pcrA"
                     /locus_tag="PB780_000354"
     CDS             complement(32618..34852)
                     /gene="pcrA"
                     /locus_tag="PB780_000354"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HDE0732430.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            35007..36566
                     /locus_tag="PB780_000355"
     CDS             35007..36566
                     /locus_tag="PB780_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HDE0732431.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(36677..38608)
                     /locus_tag="PB780_000356"
     CDS             complement(36677..38608)
                     /locus_tag="PB780_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDE0732432.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(38626..39540)
                     /gene="pfkB"
                     /locus_tag="PB780_000357"
     CDS             complement(38626..39540)
                     /gene="pfkB"
                     /locus_tag="PB780_000357"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDE0732433.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(39537..40289)
                     /locus_tag="PB780_000358"
     CDS             complement(39537..40289)
                     /locus_tag="PB780_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDE0732434.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(40449..40658)
                     /locus_tag="PB780_000359"
     CDS             complement(40449..40658)
                     /locus_tag="PB780_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732435.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(40669..41997)
                     /locus_tag="PB780_000360"
     CDS             complement(40669..41997)
                     /locus_tag="PB780_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732436.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            42133..42288
                     /locus_tag="PB780_000361"
     CDS             42133..42288
                     /locus_tag="PB780_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732437.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(42362..42949)
                     /locus_tag="PB780_000362"
     CDS             complement(42362..42949)
                     /locus_tag="PB780_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDE0732438.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(43032..44282)
                     /gene="clpX"
                     /locus_tag="PB780_000363"
     CDS             complement(43032..44282)
                     /gene="clpX"
                     /locus_tag="PB780_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HDE0732439.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(44529..45818)
                     /locus_tag="PB780_000364"
     CDS             complement(44529..45818)
                     /locus_tag="PB780_000364"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDE0732440.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
BASE COUNT        12713 a         9953 c         8218 g        15131 t
ORIGIN      
        1 ttcataagta ttcacgagaa aaatacaaac aaaaaagcgg aaataacgct catccgcctt
       61 cttgtttgtg tttatttcct cttcaaacgc ttgtctatga ttcagacttt tctctatcgt
      121 tattttcatc taaaagttct tgcattccga taatttctcg atattgatga atcttagtga
      181 taccaatgac tttgtctaca tcaatactaa aacaaatacc attacctgga atatcgatgt
      241 tgatgccttg accaatcgca tccattactt tattcgtcaa actatcgggt actaatgtca
      301 acacaatatc tttttctggc gaaatgtcca aacctaaaaa tttccctgaa tcatgtacac
      361 cagaacctct tccatgtaag atcgttccgc ctgctgcacc tgcttctttt gaaaagtcga
      421 taacatcttc gcccattcct ccgtcaacga tcgtcacaat catgtgaaga ttcaatgcta
      481 caagatcttt catttgcttt cccccaattt ttgcaatctt tctttttcga ttctgtcttt
      541 ttcatctttt cgtttataaa tatatcctag tactaaagtg gataatattg gtgtcaatgc
      601 aatcattgca accacaccaa acccgtcaat gatcggatct cttccttcaa tttgtgcagc
      661 gattgccacg ctcattgata aaataaatgt cgaacacatc gggcccgttg ctactccacc
      721 gttatcaaaa gccattgcta aaaagatctt gtccactttt cgtcctaaaa taaatgctag
      781 cccgtatcct gggatcaaga aataataaag agagaatccg acgagcaaac gccacataga
      841 caatcctact gctgcgccga tcccaattga aattaccatc agcatgattt tcgatcggat
      901 tctcccctcg ctcaattctt caacctgttt gatcattaca tgaatcgccg gctcagcaaa
      961 ccctactaag aaccccatta aaaagccaag gggaaccagt atataattgt aaggcaatcc
     1021 cgcaatagca ctgcctaaat gctgtcctac cggtacataa gccatattga ctccgtgcag
     1081 gaaaaggatc aacccaatag tcgttatcaa gaaacctttc ataatgtcaa agaaacgctt
     1141 tttgcgcata cgaaaactaa tgatattcat taaaacaaat aggattacga taggtgccac
     1201 tgccaaaatg acttccatgg taacgatttc gattccatct atcacttgct gccagaaact
     1261 cattggaaga tcacccccaa aatcatcacg gcaagaatcg gccctaacga agcaataccg
     1321 accattccaa aactgtcatt ttcatttttc ttttgtttga tcatactagt catccctccg
     1381 gcaagggcca agataaatgg aacagttact ggaccagtag tcactccccc cgaatcaaaa
     1441 gcaatcggca taaattcgga actggtgaaa aatgaggcga tcaaaacgcc tatgtagcct
     1501 atagccatca attgataata ggatagcttg aatacgaccc gaagcagcgc aaaggctaga
     1561 aatactccag tgccgacgct gacgatccca atcagcaaca ctctgccaat ctctccgtca
     1621 gatatatcat atacttgttg tcccaaaact tgcacggaag gttcagcaat cgtgatcacg
     1681 atcccgatag caaatcccaa actgaccaaa acggtcaaac ttttcttttt gatcatatat
     1741 ttccctacta gatctccgac ttccatcata gaataatctg ctccaaacaa aaagagcgtc
     1801 attccgatca tcatgattgc cgcaccaacc agaaatgaag cgaggtcttc tccttctaac
     1861 ggtgcaaaaa caaacgttaa aatgacaatc aggacagtca tcggcaatat agcagcaatc
     1921 acttctttaa aattttttag catgtatcct cctcctttac tctttaagtt taccttaaaa
     1981 tgaaagagtt ttcgagcaca aacttaaaac gttatcattt aatttttctc aacataaaat
     2041 aacacacgtc ccaaaagtat ctttttactt ggaaaaatga ctttgaagga acgcgcttat
     2101 tgtgtttttt tattttacag agccgatcat tccttgaacg aattgctttt gcaaaacaaa
     2161 aaatacgatg gcagttggta aagtagcgat gactaatcca gccatgatca ttccatagtc
     2221 tggtgtatag gaagcaccca tagcagataa aacaagcgga accgtccgtt tatcaggaga
     2281 ttgcaaagct actaagggcc ataaataatt gttccagcta ctcatgaatg tgatgattgc
     2341 agccgcggca taggtatttt tagccgtcgg catatacact ctaaaaaaaa tacttagttc
     2401 ttttagaccg tctaatcgag cggcttctac tagatctttt ggaaaagatt taacattttg
     2461 tcgaaagaag aagatcaaaa aagcagtgct aacagatggc aggatgacaa ccaagaaact
     2521 gttgattccc aaaaccgttc cgttcaattg tgaaaacatt cgatataaag ggatcatcaa
     2581 tgccgcaaaa ggaaccatca tcgatagcag tagaatattg aaaattaggt cttttcgttt
     2641 actttgataa atctcaaatc catatcctgc cattgacgaa atcagcaatg caaacacagt
     2701 ggtcaccaaa gcgatcatcg ctgaattcca aagggaacga gtaaaattca gctcattgga
     2761 aaacaaattt tgaaaattga tgatcaagtt actgccaatc tttatttttc ccgatgtgat
     2821 atccacgggt gtattcgtca ttcccaaaac catccagaca aaagggaaaa tggaaataaa
     2881 tgataggatg cttaatgctg tatactttaa tactgaccca attgtcgaac gaagttctaa
     2941 tgcttgtttt ttgacgttgg ggcgttcgat tgtttctttt tctaccatat ttttcatcga
     3001 ctttctcctc ctactttcgt ttggattatc gacaatatca caataaagga aacaataaca
     3061 aaggaaactg ctgccgcata tccaaaatta ggcgtgaatt tataactcaa attatagatg
     3121 tattgagata gggtggtcgt tgcatttgct ggtcccccgc cagtaatatt ttgtacttca
     3181 tcaaaaagct gcaatgtccc gatcgtcgaa gtaatggaag taaacaaaat gattggtttt
     3241 aaatttggga tcgtcacttt gataaactgc tgccatttag aagcaccgtc aatagatgct
     3301 gcttcataaa tctccgaatc aatattttgc atgcctgaaa ggaaaaaaat catattgtat
     3361 ccagtccaac gccaagtgat cgaaagaatg atcagaactc tcgcccaaat tggatgagtc
     3421 aaccagccaa tcggtgtgtc aatcatacca atgcccaaca acaagttgtt tacaaggcca
     3481 gattgagaaa atagactttt catgatcaga gaataactga ccatcgaagt gatacatgga
     3541 agaaagatag cggttcgaaa caacccttta tatttcaact ttttatcatt taaaaggttg
     3601 gaaataaaaa gtgctagaaa caacatgatc ggtacttgta caagtaaata caggaatgta
     3661 ttaaaaaaag cttttttaaa agttgcatct gtcaacatcc gctgataatt acctaacccg
     3721 ttaaatgtca tattgacagg gggaccactt tgaaaagacg tgatcaaagc caaaaccatc
     3781 ggaataaaaa ccatgattga tatcaaaatg actggcaaga ttaaaaaagc atgcccgttt
     3841 ttgaataaat ctttctgctt cattcttttc cctccaattc tatcattgat tatctacatg
     3901 aaaagacaag aaagacaata tctgtaaggc agacgtgatc ttttttgtct tttcgtttgt
     3961 ctgatgaaaa agcgaagaaa ttcacttttt taccaccgat caattagcca attgtgcttc
     4021 tacttgtttt tgtgtatctg ctagtacctt gtctgtctct tctccattga tgattcgatg
     4081 aagagcttca gctacgactg cttcaattgc ataggtctca tgtccgtaat tcacttctgg
     4141 tatttcagat gtccatgttg aaaaatcgtc aaagatcttt tgaccactgt aaaattcaga
     4201 agcttcttga taagcttctt gttcattcgc gctaagcatc gtagatacta gtccgatttc
     4261 tttcgcaaga gtgcccatca gttcttcatt cgaagcaaac gttgcttcta ggaaatcttt
     4321 cgctgaatct tctccagcta ctcctttgat cacataccag ccagcgccgc caaggttaga
     4381 agcttgcgct ttttgcagat tttctggaag tgcaggtatt ggtgcaatct tccattttcc
     4441 agattggttt tctgctcctt ggatcgttga agaataccaa gcgccagttg ggctagaagc
     4501 tactgctcct gattgtatcg cagttactcc agcattccag tcagaggttt gttcgactag
     4561 atcttctttc aaaagagtgg caaacaattc aagaccatac tttaaagatg cattgtcttg
     4621 gatcgtgact gtctccccat cttcagatgt ataccattct cctgcctgtt gcataatcat
     4681 ccttactcga ccaaggtcag aaggattgac ttcagcgatt ttctttcccg tcttttcttt
     4741 gacatctcgc gctacttgaa tatactcgtc ccacttcaga ttctccatat cctcttctgt
     4801 ataacctgct tctgccatca gatccgtacg atagaagttt gctgttacgc ccgaatcgaa
     4861 aggaacaccg tatattttat ctcctacttt gtttaccgca aatttatacg gtgcaaaatg
     4921 atcttccttt acaatagcag acaaattagc gaatgcatct ggataagaag tcaaatagcc
     4981 ttgtatccta taatcttcaa tcaagacgat attgggcaat ccttctgtat tcccgctcgc
     5041 taaagcggtg ttcagtttct gaacgatatc atcctgagac atcgtaacaa cttctatctc
     5101 tgtttcttcg ttctcataaa ctttttttgc ttcattcact gctttgatat tgaatgtctc
     5161 atcccacgcc cagacagtga ctttttcttc tttgtctgct gtactactag cacttccgca
     5221 tcctgttaag aaacctgatg ctgccgctaa acaaacgact ccagcaatta actttctgat
     5281 gttcattttt atccgctcct tttccatcca ttagttgttt acgtttgcta cattcatcat
     5341 accaaaattt gaaaacggtt tctttattat tcagatactt caaaaatttc tcctaaaata
     5401 ataaaaaaat acaaatcaac tatgacaaaa ggaataatct tagcatctag accaaagatc
     5461 gaaatggtga ggtttcttta aaataaaaat atccttgcat tttctcctag aaataagtat
     5521 ttttaaatta caagactatt cttaaggcgg gtaaaaaaat gccaatacgc gtaattctta
     5581 tcgatgatga acagacgatt ttagatggaa tgaaacagtt gatcaattgg tctgagcacg
     5641 ggtttcaatt aatagggact ttttccagtc cgctgaaagc gcttgatttt tctttggtac
     5701 atgcaatcga tatcgtggtc acagatgtaa tgatgcctga attaaatgga atcgaattat
     5761 cgcggctatt aaaacagcaa caacctgaaa cacagatcct tatcctctct agctatgatg
     5821 aatttgatat ggttaaagat tcttttaaag aaggcgtcgc tgattatctt ttaaagcctc
     5881 gattgagtcc tgattttttt ctaaattcct taactgccgt ttcacaaaaa atcaaaaaaa
     5941 agcccaacaa aagcccaatc accaatcgtt atgagcagat ctgcgagaat ctttcacagc
     6001 agattgctgg acaaactcgt ccattgcttg accctcttct ttttgaacac accttgtttt
     6061 tctgttatat accgaaaaac ggccattcag ttcaaaacaa ggctctaaac aaaaacagtc
     6121 gatcacccat tcattgatgg atagcgacga ttcgtttact atttatcctg tttctacctt
     6181 ttcggaagaa tccggctatg taatcaatac tgattccaaa gatacactta ggaagtttgt
     6241 tcaaaagttt cgtcaagaag tttttccagg tgattttttt gttgtttctg attcttttgc
     6301 aatccaagat atcaatgaaa tattccaaga gatcagaaat tgcaccaaag gacaacgttt
     6361 ctatcataag ggaaagaacc ttgcttatca gcatgaactt cttccgttat taaccaacca
     6421 cgagcagcaa gcaagtagtt atttaacaaa aatcgttcat aaagactacg ctttagctgt
     6481 caaagaactt gagaatttct tcacgacgat attggaacag cctattgatc cagcttattt
     6541 gaaacatgag gcaatcaatc gtttctatgc attactcaac gctttggctg acgagcatgt
     6601 tgggaatgaa gaactttctt tgctcaagat cgcccttcct tctttattag ccgatgctga
     6661 gtatttcgaa gatttttcta ttcttgtcaa agaaacactt gagaaactca cccacttttc
     6721 tgcaaatgca aaaaaagaca atttcgatat cttgactttg atctacgagt tcgtccagga
     6781 aaattatgag aaagaaatca atttacaact tttatccgaa aaatatcatt tttcatatag
     6841 ctatctctca gccatcttta ctgaaaaatt tggtatcaac ttttcaaaat atctaaaaaa
     6901 agtccgtatc agtaaagcca agctcttttt gacggaaaca cggcttactt tgacggaaat
     6961 ctgtgagaaa gtcggctata ccgaactggg ttatttctcc agagttttta aagaagagac
     7021 aggaatgaca ccttctcaat accgtaaagg aacttttgta aaatgagaaa aaaaatcaaa
     7081 gagcatgaac tctttgccaa acttctgttc attttgttca tcggtctttt tctgcaagcc
     7141 atcgtcattt ctttgtttat ttatcatcga tcaagggatg cctatatcca gctgttcaat
     7201 caatcgaatg atgtggtact aaaaaagatt caatctgaat ttgaaagttt gaatgataca
     7261 atcgagaata cattagccgc ttttgattct aatccagctg tcaaaagcta tttttccaat
     7321 gatccagccc aacatatgga acagcttcag caactaagaa caatccaaaa aatgaatgac
     7381 tctctttcaa aaatccatcc aatgatcgat tacgatgtat tgatctttgg agaaaatgga
     7441 agaacttttg tcggaaacga catgctcact gccgtatctg ccgattcttt ttttcaatct
     7501 gcgattgctc agagagtgaa tgaacgtgct gctgatacac aaatgctttt tgcacaccac
     7561 ggattgactc ttcgcgataa gaaagcaccc agcgtcttct ttgtcaaaaa actgaaaaat
     7621 acattgaatc acgtttacgg atatgctgtt ttatcgatct catccaagca actagcgaat
     7681 ctttttcaat ctgtagtaaa tcctgagata tcgaagatct ccttcatgaa cgaagaacag
     7741 acgatcatcg catcaaatga agaaaataca attgggagcc gatcaatgat tttcaaaaaa
     7801 ctaacagtag gtgaaacaac gatttcaaat ggacagcagt tgacccgtct tcctctgtac
     7861 cgccaggatt gtacattact cagtcaagtc gatcttcatt ctttatctaa ccaaatgggc
     7921 gtgatttttc cgattatttt gtataatatc gtgacacttg cttttgctgg ctcattggta
     7981 tttatgtatt tggatcgaca cacaaaatcg atctaccgct tgattgattc gctaaaacgg
     8041 ctagaggaag atggcttaaa agctactgtt cctgtacaag gaagccatga agtccaattg
     8101 ctagggaata cgatcaatca attgctaagt aatgctcagc atcattatga acagacgatc
     8161 caagacgaaa agaagaaacg ttcgcttgaa atccaaacta tgcaagcaca gatacagccc
     8221 cattttattt ataatacgct gactagcttg aaatttttga tttggcagaa aaaaacagat
     8281 gaagcagttc aaggaatcga cagtttcatt actttactaa gaagtacgat tgggaaaaaa
     8341 gaagaagtga ttccagtcaa ggaagaaatc aaaagcgtac aaagttacat cgatcttttg
     8401 agtatgcgat atggcgaaag aatctcggct aaaataatga ttcctgatga actgcagtca
     8461 ttatctatgc cgaatatgat ccttcaacca attatcgaaa acgcctatct tcatgctttt
     8521 cacaataaaa cgcaaggatt catcacggtg tacggcaaaa caaaaaatca gcttctttta
     8581 ttcgaagtga ttgataacgg cgatggcttc gatactgaaa ataccaaaca aaaaaatgat
     8641 catttttcag gaatcggcat cagccatgta gatgagcgga tacgcctaat gtatggcgat
     8701 gcatatggct taacgatcca atcagtgatt gggacaggaa cgactgtaac gatttgtcta
     8761 ccgcttcttc ctaaaaatca atcagaatat caaaaataaa gaatagccaa gcgctgattt
     8821 ttttatttta agttaaataa atcaatcagt attcttgact ttcatctacg cgtctcttct
     8881 actgtcagca ctggataaca gcccaatatt tgactttctc ctccgagcat cttgatttct
     8941 tggagcgcat tctctactaa tgtcattgga cggtccaaca acaaatcgat cacgaaaaaa
     9001 tattctccca agctagtttt caatggtcgg gattcaatct tacttaagtt gatttcccgc
     9061 cagccaaaag ctgccagcat tttatgaagc atccctggcc gattagcagg tagtgtcaag
     9121 atgacactca tttttactgg agctgactgc tggctggtca tctttcggtc accgacgatc
     9181 caaaaacgtg tctggttcaa ctcattatct tggatattct cagctaagat ctccaatcca
     9241 acatgctgtg ctgtttctaa tgaagcaatc gctgctgcgt cttctttcgg atgttctgct
     9301 acatacattg ctgcagcagt tgttgattct gttgctacta aaggaacatt aggataatgc
     9361 gtttctaaaa actgttgtga ttgtgctagt gcttgtggat gtgaaaggat ttttgtaatc
     9421 tttgttgctg gattgcctaa aagctgctgc ttgataggaa gaacgatttc acttttaact
     9481 tcgacttctg gatgtttgga aagaagatcg attgtgtgat gaacagctcc ttccaatgaa
     9541 ttctctacag gaacgacagc cagatcgact tgtttgcgaa acaaagcgcg caaacaaaca
     9601 gggatggaag catacgcagt caattgttct gtaggaaata gctgacaagc cgcctgataa
     9661 gtgaatgaac tttctggccc tagatagctg acgttcatat gcttttgatc ctttctataa
     9721 tggtatcgat gatcttttct ggtgtttgat tggtcgtgtt gattcgatgt gtcgcacttt
     9781 cttcatacca agaaagtcgt tgtgccaata atgcttttat ttcttctctt gtttttccaa
     9841 gagctaaagg tcgaatagac gtttcatctt gttggatacg atcaactaga cagtccgctt
     9901 ctgcctctaa gtaaagaaca atcggctgtg tttttaaaaa acgccgattt tctgcacttt
     9961 ggacaatccc tccaccagtc gcgataattt tgtcttcatt tgacagcttt cttaaaatat
    10021 ctgactccac ttttcgaaag cttttttcac cgtaatagtg aaaatattcg gcaatcgaca
    10081 attggatatg ttcctcaatc tttgtatcta aatctacata ctctttttgt aaacattgag
    10141 ctaacccttt accgattgtt gtttttccag cgcccatgaa accaatcagc aaaatgctag
    10201 ccatgagctt ctcctcctga cacaagttct gctaaatcgt caaaaaatgc cggataagag
    10261 attgaaacag cttcagctcc ttctagttcg caaggtccat cagctatcaa agcagctact
    10321 tgcagcatca taccgattcg atgatcgcct ctgctggaca cttttgcgcc atgcagaggt
    10381 gtcggcccat ggatgatcag accgtctgct gtcgctttta tctttgctcc cattttctgt
    10441 aattcttctg ccaccgcatc aattctgttg gtttctttta cttttaattc ttctgcgtct
    10501 ttgatgatcg tgattccttc tgcttgagta gccactaaag ctagaatggg caattcatca
    10561 atcagacgcg gaatgatttc tccaccaatc gttgctttct tcaaatgact cgtcttgaca
    10621 cttaaatcag ccgattggtt ctgttcattg tgattcgtct gggtaatcgc tgcgcccatc
    10681 tcctccaaca catctaagat accagttctt gttggattga ccccgacatt ttttaacaac
    10741 agttggcttt ccggaacaag aagtcctgct gctagaaaga aagcagctga tgaaatatca
    10801 cctggaacag ttatctgttg accagttaat ttttgcggtc cggtcactat gatcgtttta
    10861 tcttccactg tgatccttcc gccaaattga cgaatcattt cttccgtatg gttacgggaa
    10921 cgttcttttt caacgattac ggatgtgcct tctgcttgta atgccgcaaa cagcaaagca
    10981 gatttgactt gcgcactggc tacaggcata ttgtatcgga taggtgataa agagcgggtt
    11041 cctgtcacag tgattggtag atactggtca ttttcttctc cttgcaagtc agcgctcatc
    11101 tcgcgtaaag gttccatcac tcgccccatt ggacgtttag acaacgaagc atccccgaac
    11161 agagtagtcg taaaaggctg tccagccaaa atacccatca ataaacgtat ggtcgtaccg
    11221 gagtttccca tgtcaattga ttcggttggt tggacaaatg agtggctacc gcgaccatga
    11281 atcttgatca cctcttcctc ttcttcgatc tcgacaccta attgttggaa ggcatgtaaa
    11341 gtactcagac aatcttctgc gcgcaaaaaa ttttgtatag tcgtcgttcc ttcagcaatt
    11401 gctccaaaca tgatgctgcg atgggaaatt gatttatctg ctggtatcct aacagtccct
    11461 cttaatccat gtatttgttg taataattgc atttatccag cctcctattt gatccagcaa
    11521 tgataactgg tattagacat aatgattctt tttgcttgta acaaatcctg ttggttttta
    11581 aaactgattt gcagtattcc taaaatatct tccctggtct cttggatctt caaattaatc
    11641 aaagaaactc ccgcttttcc gatcagtcca gttacttctg caataacgcc aggttcatct
    11701 ggcacatcga taaataaatc aaaaaaggca ggaatcgcgc cttgcttatg gacaggcaac
    11761 tggtctcgac tttcttttgc ccgattgaaa aaagaaaaga tttccgattg gttctcagtc
    11821 atgatccatt ctgctatttg tgtcatttct tgttgccaat ctgccaatcg ttccaataat
    11881 atctgcttat tgctcaacaa aatatctgtc cacatgacag gatctgagga agcgatccta
    11941 gtgatatctc gaaagcctcc tgctgccagt tgtttggctc gcggatactc ttgattgaat
    12001 tgttctgcct gattgactaa gccagctgcc agaatatgtg gaaaatgact tagcattcct
    12061 gttatacggt catgttctct tgctgtaaga gttacatatt ttgctttggt tccgcaaaat
    12121 agttcttgca gtttttcaac atcttttttc gtcttttcca aaggtgtaaa gatgtaatag
    12181 gcattttcaa atagtttagc atctgctgcc tgtatacctg atttatgaga accagccatt
    12241 ggatgtccac cgacaaaccg aagatttttc tgctcagcgg atgtcacgac ttgctgcttc
    12301 gtactgctga catccgtaat gatgacgtga tcagacaagt tgattctttc aaggacatca
    12361 agataagcta aggttgtacg gacgggaacg gctaataaga taacgtctgc ttgttctgca
    12421 cctgtttcaa aatcacttgc aacgtaatca acgatctgct gttctactgc ttgctcacgg
    12481 gtttttgact gactatccca accaactact atcagttctg gattggtctg acgaatacaa
    12541 agtgccatag aagcaccaat cagtccaagc cccacgataa aaacatgttg tgccatctta
    12601 tttgccccct ttttagtaat gtttcaagta gtcacggtac tccttcactg cagctttcaa
    12661 ctcaacaagc gaatcacttg ggaattttga caatagctct gtcgctacca cagtggctac
    12721 aacattttcc gcaacgacgc tagctgcagg aactgctgta ctatccgaac gttctacact
    12781 tgctttgtaa ggttcttttg tatcgatatc aacactttga agtggcttgt acaatgtagg
    12841 aatgggtttc atgaccccgc gaatgacaag tggttcgcca ttggtcattc cgccttcgaa
    12901 gccgcctaga ttattggtac gtcggaaata gcctctttct tcttcccata ggatctcatc
    12961 catcacttct gagcctggta atgaaccaga agtgaatccg accccaaatt ctacgccttt
    13021 aaaggcattg atactaacca cagcttgtgc gattttggca tctagcttcg tgtcccattg
    13081 tacgtagctt cctaatccag ctggaacgcc tccaacgagc acttctacaa ttcctccgat
    13141 cgtatctcca tttttctttg tctgatcgat caaatctttt attctttgct caactgctgg
    13201 atcaacaact cgtacttctg acgcttcaga aatttctttg atttcttgaa tcgagatatt
    13261 tccaggtatt tctgcttgga ttccaccaag acaaatcacg tgtcctgcta cgatgatgtc
    13321 aagttcttcc agaattttct tggcaattgc gccgattgct acacgcatgg tcgtctctct
    13381 tgcagaggac cgttcaagca cattccgaag atctcgatga tggtatttca taccgcctac
    13441 caaatccgcg tgtcctggac gcggacgtgc aactcgccgc agtgattggt ctttttcagg
    13501 cacttctgct acagacatga cagttgtcca atttttccaa tctttatttt ctaccactaa
    13561 agtgacaggt gaaccaattg ttttgccgtg acggatgcca gacgtgatcc gaacacgatc
    13621 tttttcgatc ttcatccttc ctcctctgcc gtaaccagct tgtctgcgag cgagttcgtg
    13681 attgatttct tcttctgaca gaggtaatcc tgctggtagt ccttcaataa tggctgttaa
    13741 ttctggtcca tgggattctc ccgcagtcat ataacgcatc ttacagtcct cctaagtaat
    13801 ctttgaatgt ctctgtggga acaggaacga tttttgcttg cccaatcttt tctagcaaga
    13861 tgatattcaa ttggcttcct cttgctttct tatcatgggt tatcgcgtgg aataattcat
    13921 ctggatgact tagatcgtac gtaatcggca gatggaattt ttgaagtatc ctttctaaag
    13981 cagctgtcga tccagttgga gacaagccga ttttttctgc atgagaagtg atggctacca
    14041 ttccaatagc tactccttct ccatgactga ctaccccata tcctgctgta ttttcaattg
    14101 catggccaat tgtatgacca aaatttaaaa taagtcgatt tccttggtca aagacatctt
    14161 cttccactac ttttcgtttg atttccaacg tagcagtaat gattggaaca gcatgttcca
    14221 gaagatcttt ttcgtctttc agctcgttca gctgtttcca aagcgttgca tctgctatag
    14281 ctgctgattt gatgatttca gcgattcctt ctcgtaaacg acgtaccggc aaagttttta
    14341 gaacctcagg atcaatcaat acaccagctg gctggctaaa tgttccgaca aggtttttcg
    14401 ctttgtttgt attgactgct gtcttcccgc caatactaga atctacttgt gcaagtagcg
    14461 tagtcggaat ttgaacgaaa tggatcccac gcatgaacgt cgaggccacg aatgctgcta
    14521 gatcacccac cattcctcca ccaagtgcaa gtatcccatc gctgcgggta aaatcattat
    14581 ctgctaaaaa atcgtataac tcagctgctt gttctaaaga tttactggtc tcacccgccg
    14641 gtacgattga aaggactggt tcaaaattag cttgtctcaa ttggaacatg attttttctg
    14701 catatagtgg agcaacgttt gtatcagtga tcacagctac tttttgaggt ctccaaatcg
    14761 tttgaagcca tgatccagcc tgttctagtg cgcctttttc aatatgaata tcgtattttt
    14821 ggttatcaac atgtacagta agcattcgtt tcacttcttt cttttaaaaa gctggaatta
    14881 atcgagtgtc tgaatctttt ccattgcttc tttcatgatc gcaacttctt tcatcatgcg
    14941 tgagtaagtc ttttcattca acgattgggg accgtctgac caagcatttg caggatcagg
    15001 atggatctct acgatcatgc catctgctcc gcttgccaca cccgcacgag ccattggtgg
    15061 tacaagttcc caaattcctg taccatgact tggatcaaca atcacaggga aatgagttaa
    15121 ttttttcatc aatgggacag cacttaaatc aaacgtatta cgtgtcgctg tttcgtaagt
    15181 ccgaatccca cgttcgataa aaatgacagg agatttgtct tcgactgcaa tgtattctgc
    15241 agcattaagc cattcattga tcgtaccgga aattccacgt ttcaatccaa caggtttacc
    15301 tgttttaccg acagcagata gcagtttaaa attttgcata ttacgtgcgc caatctgtag
    15361 gatatcgctg tactcagcca ccatatcgat atgcccttca tccatcactt cagtaatcac
    15421 tttcattcca aattcatcag ccgcttgacg gatgtatttc aatccttctt cttctaaacc
    15481 ttggaaagca tatggagaag tacgtggttt gaaggcacca cctcgcagaa tttttgcgcc
    15541 cccggctttg gccattcgcg cacattctct gatttggtct aatccttcga tagaacaagg
    15601 accagccatc gtcacgaaac tgccgtcacc aatttttact ccgtctacat ccacgactgt
    15661 attgccagga tggaattctc gagaagtcaa tttgtatgtg tttaaaatac gcacagcatt
    15721 ttctactcct tcatagcgtg taaatgctcc ttcttgtaaa cttcgcgtat caccttttag
    15781 tccaatcact actcgttctg ttccttgatc gattgagact ttgaatcctt ctttttcgat
    15841 atgatcgacg acttttttta tttgttcttc cgttgcttct tttttcataa ttactatcat
    15901 tgtttttccc gctttctttt gtttacttat ctatcttggc tgattttctc tactttttca
    15961 aggtggtttt acattaattc ctactaaatg accgtgtcct gttgttctca ttttcgctcc
    16021 ttctttccta cttatgaccc agataatcaa caaaaaaaga tgtccctgta ggaaattgcc
    16081 tctacagtga acatcttcaa cgagccttaa aaagcgctgc aaaaatataa aatagccact
    16141 atagatgaat ttctctacgt ggctgtaagt cgtgtttaaa aatctttagc catcatagat
    16201 acaaacctgg tttgtttgta cccacgaagt catgaataca aacctatcta aaatagctaa
    16261 agtaatacgt attcagtttt gtaagttttc tagatttcat aaaaaatcgc tccaaactga
    16321 tgtaagaatt gtacctagta tagacataaa aaaatcgact gtcaatcatt tttatgaaac
    16381 tttttttaat tttctgatag ttaactttgt ttttcagaaa aataacaatt ggttttattg
    16441 aagaaaatag tcatgaacaa aagtatctga gaactttttt actctcttgt tcatgacata
    16501 tagaataaag cagctttgtc ttgtaaactc cgtttttcaa ataataatct aaagattgct
    16561 actgaataat aaagccgatt tcatggtcct cttcgctgct gattcggtaa ggtgactcaa
    16621 catccgctcc ccagctattg atacccccga ctcctcttac ttttgcaaaa atcgtaagaa
    16681 ccgttcgtct ttctggcggt agttcttcta tatgtgttgc ctgttccagt tcttctgctt
    16741 tgaatggtaa agcggaaaaa gcaaatggcg tatcctgctg ttcaaatcgc aacgaaaaat
    16801 cttcttgatc tttatccgta ttatccaacg tttgatttct tgttagttct acccatttcg
    16861 tttccatatg catgccgcat tcttggggaa caaggtatgg ggaaacgggg agtcctgcca
    16921 ctttataaat accttcgaca ccaccagcca tccgatccgg atatgtttcc cctgataggc
    16981 cttcatactc aaaaaatttt gctttcgttg gcataatgaa ccttaaaccc aatgcgggta
    17041 attccggtaa tcctttttgt ccataataat gcatattgac aagcatttta ccctctccag
    17101 cgatttggta ggaaattgtc acttgggttt ttggtactgt gttgatttcc agtgtaaaaa
    17161 tgatttcaac cgtttcagcg tattcattac ttgaatattg attatttact ggagcaatcg
    17221 gcaaatcgat ctttttattg tccaccagta cttctatttc tttgactttt ggatacaatc
    17281 cggctcctaa ccacataccg gatttaaatc caaatccgct acctctgtca ttatcggtcg
    17341 ttgctcgcca aaaaactgga agtgtttccc ggtatagcca ttcttttccg tttttattga
    17401 gcgattccag tccgcctctt gtataagaaa aaatataatg cgtattcccc gatttgacac
    17461 ctaatgtaca gtcgccgaag acgattgcaa ctttttccgt atccatatga cttttattta
    17521 ctgtattttt cataataata tcttacctcc tgcatggcgg gtttttcttg tccatctgca
    17581 aaaacgatcc cattccctga gaattcatag tcacttggtt tatcgtcaaa gtcaccgcca
    17641 tagcgcatca cttctctgcc tgtgattgga tcagtcactt ggatcgcctg atctttaaaa
    17701 tcccatatat agcctccttg gtacatttca taacgatcaa gcaacgtcat gtaagaatcc
    17761 attccaccta acgaattccc catgtcatgc atatattcac ataagatgaa cggtttttcc
    17821 gctttatttt caaggtagtc aatgatctct tctggtggcg catacatgca gctttttata
    17881 tctgaaatcg aatcttcgaa tgctggattt ggaaaattcc cttcataatg gacaagtcga
    17941 tcggggtcta cttttttgaa gtaatcgttc atttgtcgaa tgttttcttc cgcataagat
    18001 tcatttccaa gagaccaaaa aagaatactt ggatggtttt ttaatgtttc gaaattcgtt
    18061 tttgctcgat caagaacagc cgcctgccac tgaggtactg cacctggtac gttgtaagaa
    18121 ggctcaaatg cgcccatttt ttgccatgag ccatgggatt cgaggttcgt ttccgccatc
    18181 acatggattc ctgcttggtc gcataaataa taccaagtga tctggttggg gtaatgagat
    18241 gtccgtacgc cgttgatctt tgcatttttt atgatagcca aatctttttc catatcttct
    18301 atagtgatgg cgcgtccttt tttcggatgc cactcatgtc gatttacccc gttgataatc
    18361 atccgttttc cattgaagta gatgatttta tctttgattt cgattttacg aaaaccgaag
    18421 gaatagtcaa tatactccgt tactcttttt tcttcgtcta tcgtctccaa ttcaagctta
    18481 tataaattgg gctgaaaatg cccccacagt gccacatttt caaatccatc gatgcgaatc
    18541 gtcatttgct cttttaacgg atattgctgt tgatagattt ccttcccatc aggatctagt
    18601 atcgctacat tcagctgact gtttttggct acttgctcaa aagacaaatc tacttcaagt
    18661 ttgccatttt gacatttgtc atctaagcga ggtttgatcc aaagatcttc taaatggttt
    18721 tgtcctcgag caactaagaa gacatctctg aagatcccaa aaaatcggaa aaagtcctga
    18781 tcttccaaga atgcagctgt acttcttttg taaacctcca cagctagaat attttcttct
    18841 tccaaataag gtgttaaatc aaattcagac ggagtgaacg aatcttcggc atacccgata
    18901 aaatgaccat tcaaccaaat aaacattgct tgttctacac cagcaaaatg aatcgagatt
    18961 tgttttccct ttagaccttc ttctaatcga aaagtcgtca aatacgaacc aaccggattg
    19021 tctttagcag ctgaaaagat cccttccgcc tctttctcag ggcttgtcgt gaaaggggga
    19081 cggcgaaata catgtccctc ccacggatac atcgtattga tgtaatggat cttgtcataa
    19141 tttgctaatt caatatgact aggaacagtg atttcatcaa aagaacaggc atcaaaatct
    19201 tttttataaa atgtttttgg tcgtaccgaa ggatttggcg aataacaaaa acgccatgat
    19261 ccatttaaag actgtttaag ggaggatgtg gtttctactt cttcccttgt tgcataacaa
    19321 acatgatcgc tatgtgcatt tgattggttc actctaaaaa cttgtggatc atctaaccat
    19381 tttatatttg ctttcatact tttttccttc tctctcataa ctttttgaaa tgaagcagtt
    19441 ctgtttggaa atcaccttga aaatcgggga agtaaagtcc aactgtcatc aattcggccc
    19501 ccgaaaatat ccgcttcgag gtctgttcct gataaagtgc ctcatcatct aaccccatca
    19561 gatatacttc atggaacgct ggttgagctg aagctagtat cctccctaaa aacacaatag
    19621 cttcatcttg tttaggagac acaaacatcc atgcggcaat attttcttca aaaggactcg
    19681 ccaaacgata ataatggcca tactggatca ccggacgaat cgtttgatag gatataattt
    19741 ggtcttttac ttgctttttt tcttcaggag acagctttgt caaatccagt tcataaccaa
    19801 aaacagcgct catagctgtt tctcctcttg ttttcaacga agtagatctg tcggtctgat
    19861 gatttggtac agctgataca tgagctgtga tggacgaaat cggataagca aggcttgttc
    19921 catattggat tttcatacgc tccactgcat ccgtgttatc acttgtccat gactgcggca
    19981 tataataaat aaacccggca tcaaaccgtc cacctccacc agaacagcct tcccataaga
    20041 tttccggata atcagttgtc aaatgttcaa gtagttcata aagcccgagc atataacgat
    20101 gcgctacttc tccttgtctc tgtgggtcaa gggtaatcga atagacatct gaaagcgaac
    20161 ggttcatatc ccatttgatg tagtcaagag gaatcgtatc taaaatcttt ctcatttgtt
    20221 tctcaatcgt ctgccgaaca tctaaccttg taaaatccag tacatgttgt gatctggaag
    20281 cagaaggcat tctacctggc tcctgcataa gaaaatcagg atgtgttcga tacagttcag
    20341 aatcaaccga gatcatttca ggttcaaacc ataaaccaaa tttcaatcct ttgctatgag
    20401 cataatctgc tatttcacgt aatccatttg tcaacttgcc ttcatactcg aaccagtctc
    20461 ctaatgaaga attgtccgca tctctttttc caaaccagcc atcatctaat acgaacattt
    20521 cgatgcctag ctcctttgct tcatcaatga tcgccttgat tttttcacta ttgaagtcaa
    20581 agtaagtggc ttcccagtta ttgaccagaa tcggccgttc agcaaattga tgacgacctc
    20641 ttgcgattct ttcacgtagc aactcatggt ggatctgact cattttgttc aatccctgac
    20701 ttgagtaaga aaggatcatt tcaggtgttt gaaaagactg gcctgcattc aattcccacg
    20761 aaaaacgatg gctgttgatc ccaccgacga ctctcaattg attgatctga tctttttcaa
    20821 gttcgaaaga atggttgccg gaataaacaa attgaagtcc gatcgcttct ccagtatttt
    20881 ctgtcgtatg atgatgaact aaagcaataa agttattcat atggtgactg ctcgttcctc
    20941 gacggctttc aaatactttt gttccagata aaacagattg ccgtgaaatc tgtctttcat
    21001 tgacatgtgc tcctggcaaa gcaatgactt catcaaatcg cctagtatgg gcaaagtcta
    21061 gctggaaact agccgctttt tctagtttga ttgtttctcc ggtttcattt cttatcttta
    21121 cagatcgtgt gatcactgct ctatcagtaa atatggtata gaacaaatcg aaataaatcg
    21181 ctgtttctct atccttaagt gaaatcacca acgtttgtgc ctctgacttg tccatcacat
    21241 aagtttgagg caagccttcg atctctggct tcccatctgt aatataatgg ctgtcatatc
    21301 tcagatccag taagtctgat ccattcttgc gctgaatgat cgatgcgggc gttcgaaagt
    21361 ccatgctgcc atgagaagag tattcttgcg gtagtgtatc tttcgacaga gaccggtcag
    21421 gatttaaggg aacatttcct gaaaatccac gatctcttcg gggataattt ttattgtctt
    21481 tgtactgctt gactggtttg ccaaaataaa tatgcgccaa tacattgctt tcttctatct
    21541 ttatcagata agaaagaaat gtatttcgta aatggaagac tcggctcttt tcatcaaata
    21601 agatgaaatt tttatctgtt tttctatgcg taatatccat catttcactc ccaacaaaac
    21661 ggaaaccgtt tacttttttt tataattcct gtatactgtt tactaaacaa ttttgtcaat
    21721 aaaaattttt tatttttttg aggagtgtgt aaaaatggtt ggaattagag atgtcgcacg
    21781 aaaggcaaat gtctctcctg ctacagtttc ccgtgtgttg aataacgatc agacgatcca
    21841 tgtggctcag actactcggc aacggatagt agaggcagct gctgcgttgg attatgacat
    21901 cagtactcgt aaatatatca aaaagagaat gccttcgatt ggtgtgatct ctacgatcgg
    21961 caaagcgagc gaagaacagg atatctatta caaacatttg cgagtaggga tggaagaaga
    22021 agctcgacgt ctgcatttag ggatgaatcg aatctataat ctatctgata atcctaaaca
    22081 atgggaagat ctagatcagt taggtgcggt gatcgttgtt gggactgtta cgaaagaatc
    22141 gattaccgcc ttgttaaaac aaaacatgca aatagtcgtc gtggataacc ctgacatcca
    22201 gcaagaggtc gatatggtgt atgcagatct tgaacggatg actaaaaccg tactggaatt
    22261 gttcttagaa aatggtcata aaagaatagc ctatatcggg ggatataaag tcgatatgga
    22321 tgaattcggt gggaagcaga tttctataaa tgaaaagagg atgcgtgcct atcagcattt
    22381 tatgatcgat catcacttag aaaaatatat gtcttatcat ctaggtggct ggacagaaaa
    22441 acaaggtgag gtacttgcga ctaatctatt agaaaatgaa tcccctcttc ctactgccat
    22501 tcttgtcggg agcgatccaa tggcaattgg tgtctatcgg gcattgaaac aaaaaaatat
    22561 ccaaattgga gaagagattg ttgtagcaag ctttgataat atcgagacaa tcgcaaacct
    22621 ctcccctggc ttgaccagtg ttcaaatcaa tgcgaaatca attgggcaag cagctgttcg
    22681 aatggcattg gagcgaattg acggtatacg ggacgaacca atcattttga cttatcctgc
    22741 acgattgatt gttagggaaa gttttatccc aagaacaaaa aggtattgaa agagaggttg
    22801 tgaaaagccg cttcgtttct tcatactcaa aacgaaacgg ctttttcaca acctcttact
    22861 tatacttatt cacaaaatcc aatgtctgct ggtaacaatt ttgcgattct tttagcgtgt
    22921 aatcaaattg gtattcatgg gtaatctctt tttttgtatc tgtataaaac aaggattgca
    22981 cgggtacctt taattctgtc aaccgatttt ctaatgcttg accttgctcc tggaatgagt
    23041 atgcatttcc gtcagttata tacgttggcg gaaattcctt agaaacatgg tcagccacac
    23101 tagcttcttt caattgctca ctcgttttcc aatcttttgt tccaatcaac gaccacgcaa
    23161 cggttttgac gaaaaacttc ataaatcgat catccgaatg ctgctgtgct gttagcttca
    23221 aatcaacagg tccgcaataa gaaatggttc ctttcagact atcttttgga agtagctgtt
    23281 tgattttcat ttgtttagca tacgcatcat tcgtttgtat agctgcatat tgcaatgcga
    23341 tttgtgaacc tgcactatct ccaccaaaaa ataattgttc catgtctagc aatttgtcgt
    23401 ctttttcttc agctaataaa tctttgatca gttcattcac ttgaagaact tgattagggt
    23461 attctgaatc aggcgctaat tcataattca tcgcaaccac tgcgatgttg gcgtctgaaa
    23521 ctaattttgt cgcaaattct ttgacacccg atttatctcc gccgacgaat cctccaccat
    23581 gtacccaaaa tagaacagga acaggcccct tgctgttttt tggatagtag atgtcataag
    23641 tattctcttt atacttggac tcatacgtca aatctgtttt tttcgccacg tttttttctg
    23701 cttgatcgaa cgttttttta tcagtgattt gaacagtatt tgaaaacata tggttgatca
    23761 agaatgctcc aggtctgggg gaaagctgga aggcaagaaa gattgcaaac gcgatcccaa
    23821 gaatgattcc gaagaaccat ccgataaatt taagaatttt ctttttcata aggctccctc
    23881 agtagttagt attaagcatt ctgattatac catagtgtta tttcctatca agctctccta
    23941 gatgatttaa tgattttccc caaacttctt ctgcaatgct tttgacaaaa gaaatttttt
    24001 ccattgttct tcaaacaaaa cattttcttc ttctgttgaa gcaaatccac attgaggact
    24061 taagcacaaa cgatccaaag gcagatatcg tgtcgcttcg tagatccggt caatcacttg
    24121 ctggtgctct tccatttcgc catttttcgt tacagtcaaa atctaagaca actaatttat
    24181 cttcagagac tttctctaaa agaacaaaac tcccagcacg ttcactatcg tattctaaat
    24241 aataggcacg gacattttct tgagtaaaat aatgggtctg ctacgtcgtc atcccccctg
    24301 atgccgccaa aatcgaataa taatttccgc gtcaaacatg tatatttagt gttgaatcat
    24361 ttgagaatat gaaaataaaa atctttttga tcaatcagtt aatcaaattg aaataacgaa
    24421 aaatctgaga cagaatgacg ttcattctga cccagatttg attttgcaca actcaacagt
    24481 ccttattggc aaatcgccaa aataatgtag actaactaaa aagactaaga aacaataaca
    24541 aaagttaaca cttcttagtc tttctttagc acgtctattt attaaagatt ttagggcctt
    24601 ttctcatgtt tttacgatca ttcttaggtg tttccatttg tttggatcgg cctccacctt
    24661 gtttcttttt gatcaattct ttcatttttt ctttttggtc catttttatt ctcctttaac
    24721 gaatgaagcg actaattcga tatgatgggt ttgcgggaaa agatctacag gctgaacttt
    24781 cttcacggca taacctgctt ctgcaaataa cttagcatct cttgcaaaag ttgccggatt
    24841 acatgagata tagacgattc gttctggtga catttcgatt gctgattcaa taaaatgatg
    24901 atctaatcct tttcgtggag ggtcaactac gatcacagat ggtttgatcc ctttttcttg
    24961 ccattttttc ataacttttt ctgcttttcc gacttcataa tgaacatttt cgattttatt
    25021 atttaaagca ttgaactgtg catcttcaat tgcttctgga ataacttcca tcccatacac
    25081 cttatccact ctgtctgcta aagacaagcc aatcgtacct attcctgaat aagcgtcgat
    25141 cacaatgtct tttttatcta actcggcaaa ctcgatggct gtttggtaca gatactctgc
    25201 ttgttgagta tttacttgat aaaatgactt tgctgagatt cgataggttt ttcctaatag
    25261 ctgatcttcg atatagtctc tgccatataa aactttttct ttgtctccta aaatcacatt
    25321 tgttttttct tcattgatat tttggatgac cgaaaccact tctggcaatt cttcatgaat
    25381 agcttcagca atttccttgg atttaaagaa tttttcttta cgagtaacta aaacgaccat
    25441 catttcatgg gaataatgac cacgacggat aatgatgtga cgaagctgtc cttcattttt
    25501 ttcttcatta tatcctcgta cttggaaccg ttgcaagata tctctgacta ctttgattgc
    25561 ttgatcgatg acaggatctt ggatataaaa atcttcgata gggactagat catggctatt
    25621 ctttttgtaa aaaccggtgg ctaactgtcc atcgatacgg cgaacaggga tctgtgcttt
    25681 attacggtag ccgaatggtt ctgccattcc gaccgttgga agtacaggaa cttctggcat
    25741 cttagcaatc ttcgccatta catgctctac ttgtttttgt ttgaagacaa gctgttgttc
    25801 atacttcaaa tgggctaaag gtgcgatacc tatgcgaagt aatgcattgt tttctacagg
    25861 ctgacgatca ggtgattgtg taataaatgt ttcgactttt ccatatccaa acttattgcc
    25921 tgcttttact actagtattt ctacttcttc tccagggagt gcattttcga taaataatgg
    25981 ataaccatcg atttttgcta cccccattcc ttcataactc aagtcgacga tttcgactgt
    26041 atatcgttcg tttttcttta ctatttgctg ttctgtcata ttttgctcct ttaagcctat
    26101 ttttcaatcc tactgaactc tactataaat cagctaatta cacaactaaa accggcactg
    26161 accttactac gttcagcacc ggcttttgat taattctttt gattaggtgt ttcgtctgct
    26221 atttttccgt caccgtcgat atcttcatcc gttaaacgtt cgacttcttt tacgaattct
    26281 ttgctgacaa cctctaattc ttcttcatct gtacctgtga ttgcttcatc aggaatctga
    26341 tcggtattgg caaagaactc aatgtgttga tgaagatttt ggaaatgcat cggtgcttcc
    26401 ccgccgtatt caccatctaa gttgatcatt actggtcgat tgatatcatc ttctgtttca
    26461 acgaacaaac tgcttgtttt ggtatagatt attcttggat catcgacatg tcgaccgcca
    26521 ttcagcatga gtgctgcaat atgcaagatc tcaaagatat ttgctgtctt cacaatgatt
    26581 aaagaaaact tcccatcatc caatttggca tctggcgcga tctgttcgaa tcctccaaca
    26641 gagttcgtca gccctaggaa aaacatcgaa gcatttccga tatattcccc ttcatcatac
    26701 ttcaagcgca ttttgattgg ctttactcga gggagcatct ctgctccttt cgcaagataa
    26761 gcaagataac caaaaatact ctttagttct gatggaactt cataagtcaa ttctgtcaaa
    26821 tagccacctg cagcgatatt gatgaagtaa ctgttatcag aaaggccgat gtccattttt
    26881 acggtttggt gtttttttat cacttctgct gctgctttaa tattatctct aggaattttt
    26941 aatgctcgag catagtcgtt cgttgtacca gctgggatga tggccatttt aggacggtgt
    27001 tcaagcggag cgattccatt aaccacttcg ttgattgtgc catcgccacc agctgccact
    27061 actagatcaa aacctagttc tgcgactcta catgcttcat tcttggctga atctggttct
    27121 ggtgtagttg cataggcgct ggcttcaaat cctgcttctt ctaatgcctg taggatatcc
    27181 gccaaatttt ttttcaataa ttcttttccc gaggtggggt tataaattac gcgtgctttt
    27241 ttcattttca gcctctatcg tttcgctaat tcatctttaa gtagcttatt cacgacacca
    27301 ggatttgctt gtcctttagt tgctttcatg atttgaccga ctaggaagcc aaccgcacga
    27361 tcttttccat ttttgaaatc atcgacagat tgttggttgt tatccaaaat ttcattaatg
    27421 attggcagta gtttgttcgg atcagataat tgcacccaac catttttttc aactacttct
    27481 tgggcttctc cgccatttgt gatcaattca cggaagactt ttttggcgat ttttgagctg
    27541 atcgttccat cggcgatcaa acgaatcatt cctgctaagt tctcaggtgt caattttgtc
    27601 tcagctaatt ccagtttctc actgttcaag taagcagaaa cttcacccat caaccagtta
    27661 gatgcttgct tggcatctgc accattttcc aaaacagctt caaaaaaatc agacatttct
    27721 tttgtaagtg tcaataccat tgcgtcatac tctggaagtc caaactcacg gatatagcgt
    27781 tcgcgacgag aagctggcat ctcaggcaaa ctagctttta ctttttgaat ccattcgtca
    27841 tcgatcacga atttcgggat atcaggctct gggaagtaac gataatcact agacccttct
    27901 ttgacacgca tcaaaactgt tttattcgtt gtttcgtcaa aacggcgtgt ttcttgttgg
    27961 atttctcctc ctgaaagaag aacttttgct tgacgttttt cttcaaaagc taaacctttt
    28021 ttcacgaaag ataaagagtt caagtttttc aattctgctt tcgtaccgaa ctcttcttgt
    28081 ccataagggc gaagagaaat attggcatca cagcgcatag agccttcttc cattttcaca
    28141 tcgctgactt ctgtaaattg gataattgat cgcaatgctt ctagataagc gtatgcttct
    28201 tctggtgaac gcatatcagc ttcagaaacg atttcgatca aaggcgtgcc ttgtcggttc
    28261 aaatcaacgt aagaataacc gccaatacca tgcatgtttt ttccagcatc ttcttctaaa
    28321 tggacacgtt cgatccggat ttttttcttt ttaccttcta cttcgatttc gatccatccg
    28381 tcatgaccga ttggttgatc aaattgggaa atttgatatg cttttggatt atctgggtaa
    28441 aagtaatttt tccggtcaaa atgagtatct ttagagattt cacagtttaa tgccaaagct
    28501 gctttcatcc cgaattccag cgcagcttta ttcattactg gcagcacgcc tggataaccc
    28561 cagtcgatca cgtttgtatt gctgtttggt tctgcaccaa aatgagctgg cgctggagag
    28621 aaaatttttg agttggtttt taactcgacg tggacttcta gtccaatgac tgtttcaaaa
    28681 ttcattcgtc tttccctcct aaaatagctg gttgttgttt atggaattct gttgcttgtt
    28741 caaacgcata tgccgcttga tacatcgttt tttcatcaaa ataattaccg atgatctgca
    28801 atccaactgg taatccttct gataaacctg ccggtacaga catacctgga agtccagcca
    28861 agttgacagg gatcgttaag atgtcactca tgtacattgt gattggatcg ttaatgttct
    28921 caccgattcc gaatgcaact gttggtgaag atggcccgat gatcaagtca taatctgcaa
    28981 agactttttc aaaatctcgt ttgatcaatg tacggacttg tcctgctttt ttgaagtaag
    29041 cgtcataata acccgcgctt aaggagaatg tacctaacat gatacggcgt tttacttctt
    29101 caccgaatcc ttctgaacgt gagttgacat atacatcatc taatgatttc acattttcag
    29161 agcgataacc gtagcggata ccatcgaatc gttgcaaatt ggaacttgct tctgatgagg
    29221 caatgatata ataaacagcc acaccgtatt ttgaatgagg caagctgact tcttccacaa
    29281 cagcacctaa tgaacggaat gtttctgctg ctttcaatac agcttctttt acgccagggg
    29341 ccacaccttc gcctaagtat tctttaggta aagcaatctt cattcctttg atgtcgcctg
    29401 tcagtccctc tgcaaaatca ggaactgaaa cgcctgaaga agtaccgtct ttttcgtcat
    29461 aaccgctgat agctgtcaat gctaatgcat tatctttcac cgtacgggtc attggtccga
    29521 tttggtcaag agatgaagca aaggcaatca aaccaaaacg tgagacacgg ccataagtag
    29581 gtttcatccc tacgatgccg ttaaaagctg ctggttggcg gatacttccc ccagtatcac
    29641 tacctaaaga aactggaatt tgtccagctg caactgctgc tgcagaacca ccagaagagc
    29701 caccaggtac ttttgttggg tcccaagcat tttttgtttt cttgaaataa gaagtttctg
    29761 tacttccacc catagcaaat tcatccatgt ttagcttacc aacagggatc atatctgctt
    29821 gatagatttt atccatgacc gttgcatcat aaattggttc aaaattgtaa aggatcttag
    29881 atgcagcggt tgtcaagata tcttttgtta cgatattatc cttgatacca attggaatac
    29941 cagcaaggac gttgctttca tcaatccctt tttcatctaa agcttttgcc tgttccaatg
    30001 ctttttcttc gttcaatgta atgaatgcct cgacctcagg ctctgtttct ttgattcgtt
    30061 caaatgttgt ttttgtcaaa tcttgtacag tgatttcttt cgatacaagc atatgatgca
    30121 gttcttctag agaacgattg tgtaattctg tcattatgcg ccagcctccc cattatcgat
    30181 gattgcgggt actttgataa agccgtcttc tgtttccggc acatttttca ataattcatc
    30241 gcggttccat ccagcttgcg cccgatcttg tcgcatcacg tttactgttt caaccacatt
    30301 cgacgtaaat ggaacacctt ccgtatctac ttcttccagc atttcaacca tatcgatgat
    30361 tttacctaat tggtcggtaa aacttgcaag ttcttcatct gcgaacgcaa gttttgctaa
    30421 tttggcaacg tgtttcactt gttcttcact aatcgccata ctaccccttc tttctaaacg
    30481 agttaaaaac tttgctctct attttaacac aagagtgccg tgtctgaaat tgtttattga
    30541 aatttgtctg aaaaaactca tttttcattc ttctactgag cgacttttag ccaatgcatc
    30601 tgccatttga tcttctgtcc aaacttcgat tcctaattct tgtgctttgg tcagcttgct
    30661 gccagcatct tctcctgcta ccacgatatc cgtttttttc gatacgctac cagtcacttt
    30721 cccgccaaga ttttcgatcg tctcttttgc ttcttcacga gtaaatcgtg ttagtttacc
    30781 agtaaggact actgtttttt ctttaaaagg agactcgact tcttgcaatt gggcatttcg
    30841 aagccctttg tattcgaaat tgacgcctgc ttgttttaat tcattcatca attcatggac
    30901 ttcttcgtta ctgaaataag tgactacact gtcggcaatg atatcaccga tcgtatcaag
    30961 cgcaataatc gactcatagt cacttttact caatgtttct agatcaccga aatgttctgc
    31021 taaaattttt gctgctttcg ctccgacatg tcggattcct aacccgaaaa tcaaccgctc
    31081 gaccgaatta tctttgctgt tgtcgattgc tgtcaaaata ttatttgctg atttttcttt
    31141 tattttgtcc aacgtcaata actcttcttc tgtcaatttg taaagatcag caacatctgc
    31201 tactagtttt ttatcataca tctgttctag tacgcgcggc cccaacccat cgatattcat
    31261 cgcatttctt gaaacaaagt ggttcaaacc ttctttcatt tgagcagggc atttaggatt
    31321 gatgcaccgt aaggctactt cttcatccaa atggacaagt tcacttccac aaactggaca
    31381 atgtgtaggc aattggtatt cttcgctgtc tttaggtcgt ttatctaaca cgacttgcga
    31441 aacttctggg atgatgtctc ccgctttata aatcagaact gtatctttca agcggatatc
    31501 cttcaactta atataatcac cgttatgcag gctggcacgg cttactgtcg ttcctgctac
    31561 tcggactggt gtcatgatag ctgttggcgt tactacccct gttcgaccga ctgtccattc
    31621 gatgttttcg attagcgttt caacttcttc tggcggaaac ttgtaagctg ctgcccatct
    31681 cggtgctttg atagtaaaac caagttgatc ttgtagcgag aattcattca ctttgatgac
    31741 gattccatcg atttcatagg gcaaatccac acgtttgtca tgatattctt caatgtatga
    31801 ccacacttca tcaatcgaat gacatagacg tttttcccga ttcgtgtgga atccaatttt
    31861 ttctagctct tctagcgcat catactgggt ttttgcttgc ataggaccga aatcagctac
    31921 tgtgtataaa aaagtgtcca gatttctttt cgctgtgatt ttagaatcta actgacgcaa
    31981 agaaccagct gcggcgttcc gaggattcgc aaagatatct tttccctctg cttctctgtc
    32041 ttggttcaat tggacaaatg accgttttgg catgaaacat tcgcctctta cttcgatgtt
    32101 cattggttct tttaatttga ttgggaccga ccggacagtt tttaggtttt cagtgatgtt
    32161 ttctcctaca gttccatccc cgcgagtcgc acctctcacg aaatttccat cttcataacg
    32221 caaagaaacg gaaagtccat ctatttttag ttcacagcaa tagtcaacta cacgtcccac
    32281 tgctttttgt acacgttgat caaaagctat cagctcttct ttgctgaaaa cgtcgttcaa
    32341 actgtaaagc ggaatatcat gagtgacttt ttcaaaacct tctaatactt ttccgcctac
    32401 tcgttgagtg ggagaatcag acgtgatcaa atcaggatat tgttcctcga ttgctactaa
    32461 ttctgcgtat tctttatcat aaacatagtc ttccactgtt ggtttatctt ctacgtagta
    32521 ttcgcgagac cattggttca gtcttgtacg aagttcttct gctcgtttag cagcttcgtc
    32581 aatcgtcatt tcatgttcca tcatttaccc tccactacta agctttttca attggcgcaa
    32641 aggcagccaa aagacgtttt actccttgac ttgggaaagc cacatctagt tctagatcct
    32701 tcgacgtccc gcttactcgg acgactgttc ctagacccca ttttttgtgt ttgactttat
    32761 ctccaggttt ccagctgtta ttttcccctc cagtagcact tttagaggga acaaccgctg
    32821 tctctggctg tttatagcta tatgctgttt ttttagcgaa tacaggcgtt tttttaggcg
    32881 taggacgcat gccttcgatt tccagtaact cctgatcgat ttcttctaca aaacgagaag
    32941 gtcggttata ttgcgtccgt ccatataatg ttctggagaa agcatttgtt aaatacaacg
    33001 cttcttctgc tcgcgtgatc cccacatatg cgagacggcg ttcttcctcc agttcgcttt
    33061 cttccatcaa tgctcgagat aaaggaaaaa ccccctcttc tagaccaatc aggaatacaa
    33121 ctggaaattc taatcctttg gctgcatgca aagtcatcaa tgtcacttgg gaagcatctt
    33181 cttctaaatt gtcaatgtcg gaaacaagtg ccagatcatt taagaagacc gttagttttt
    33241 cttctggtgc atcagcgtct tcttcatttt gctgttcgaa ttgtttgtcg aattcttgtg
    33301 tgaccgttaa aaattcctcc aagttttcga tgcgtgcttg tgcttctaat gtattttgga
    33361 tttttaagtc ttctagatat ccgctacgat ccaaaacctc tttcgtcaat tctgtcactg
    33421 tcaagtatgg gatcatctga gtgacttctt ggatcatctc tccaaatgca cctaattgct
    33481 gtcctgcttt tccaccgata tttgccagat ccacattttg cgctgcttca agcagtggcc
    33541 attcatgaag agaagcaaaa ctgcgtagtt tttcgataga tcctggtccg atgcctcgtt
    33601 tgggcgaatt aacgatacgc tcaaaactga ttgaatcttg aggatttacc agcacattca
    33661 aatacgctaa aatatctttg atttctttac gatcgtagaa cttgtgccct ccaaccattt
    33721 tataaggaat atttgctttc aacagagttt cttccattac acgagattgt gcgttcgtcc
    33781 ggtacaagat ggcaaagtcg ccgtaattcc gatgattgct tcgaatctct tcttgcatcc
    33841 ggtctacgat aaaacgggtt tcgtctcgtt cattatctgc acggtagtaa gtgatcttgt
    33901 ttccttcttt gttttctgtc cacaagtttt ttggttttcg attgctattg ttttcaatga
    33961 cttggtttgc tgcgctaagg atgtttttcg tggagcgata gttttgttct agcaaaatta
    34021 ctgcagcatc tggataatct ttttcaaaat ccaagatgtt ctgcatatct gcaccgcgcc
    34081 agccgtaaat actttgatct gcatctccca cgacgcacag gttcctaaat ctacctgcca
    34141 ataaattgac tagcgtatac tgtgcgtgat ttgtatcttg gtactcatct acatgaatat
    34201 aatggaattt gttttgataa tacgtgagtg aatcaggatg ttcttcaaat aatcgaatcg
    34261 tattcatgat caggtcgtca aagtccatac attgattata ttgaagctct ttttggtaag
    34321 cagcataaca ttttgccgca atctcttcgt aataactgcc ttgcattttt gcaaaatcct
    34381 gaggggtctg caggctgttt tttgcttgag agattgttcc taatatactt cgaggatcat
    34441 attttttcgg atcaatgttc agttctttta gtatccgttt catcaaggtc agctgttcag
    34501 aagaatcgat gattgtaaag ttccggttat acccgataaa atcaacatct cttcttaaaa
    34561 tacgcacaca catcgagtga aaagtcgata cccagacatc ttcgcctcca gatgcgagaa
    34621 tcgcattgac acgttctttc atttcttttg cagccttatt ggtaaaagta atcgctagga
    34681 tgttccaagg attgacttct ttttcctcaa tcaaataggc aatgcggtgt gtcagcactc
    34741 tcgtctttcc acttcctgca cctgccatta gcagtaatgg tccgtcagtg tgaagaacag
    34801 cttctttctg acgggggttc atcccgttta ataattcagc tttggatgac attaacaaca
    34861 tcctttcttt tatcaatctt gttaattata gcattttctt ggctttcatt aaactagcta
    34921 gttcaaaatc atttgtttct ttttatggat ttttccttgt caattcgttg atttcgcgct
    34981 aaattaaaag aaagcgaggc gtttgaatgg aaaaaaaagg aaaaatcaag aaattagata
    35041 agactcagat atattttatt ctaaaaggaa tatttgttgg actgctgaca ggaatcgtcg
    35101 ttagcctgtt tcgattatct atcgaaaagc taagcgattt agttcgaagt atctatgaaa
    35161 tgagtcggga acagccgatt tacttaattg gcctagctat ttgctgcatc cttgcagcat
    35221 ttttcgtggg ttatctggtc aaaaatgaac cggatatcaa gggaagcggg attccacaag
    35281 tagagggaca gcttcgcggg gaattatcaa tgaactggtt ctctgtttta tggaaaaagt
    35341 tcattggcgg tgtactttca gttggtgcgg gcctttttct aggtagagaa ggaccttcta
    35401 tacagcttgg cgcatccatt ggacaaggag ctggacaatt gtttcggagt ccatcttcag
    35461 aagaaaagat tttaatttct agcggtgcaa gtagcggact tgcagcagca ttcaatgctc
    35521 ctatcgcagg attactcttt gttttagaag aaatccatca ctccttttct ccacttgttt
    35581 ggctgacatc atttgcttca gcaatcactg ctaacttcgt ctctctttac tttttcggct
    35641 tgagacctgt tttatatcta ggtgaccttc cttctttgcc gttgaaatat tatggttcct
    35701 tagtgatctt aggagtactt ctaggcatct taggttttat ttatcagaat gtcttgctgg
    35761 ctttgccaaa attttacaac cgattgcctt taccagctta ttttcacggg ttcgttcctt
    35821 tcttgctgat tttgccaatt ggttatttat ggcctagtac tctaggcgga ggcaatcaaa
    35881 tcgttttagc ttttggacat gcttccttgc caatcattat gattatcggt ttgtttgttc
    35941 ttcgattcgt tttttccatg atttcttacg gtgggggtct tccagggggg attttccttc
    36001 cgatattgac attgggggct ttaatcggta gcttttatgg aaacattatg gttgatttcg
    36061 ggatggatcc aatctacatc aaggattttg tcgttattgc catggctggt tatttcacag
    36121 cgattggaaa agcaccgctg acagcaatta ttttagtgac agaaatggtt ggctctctta
    36181 atcatctgat gcctttagga ttggctgctc ttgttgctta tatcgtcaat gaccttctcg
    36241 gcggtaatcc aatttacgaa tcattattgg aacgtctact tggaaatact cagaccgcct
    36301 cactaaaagg tgcaaggaca acttttgatt ttccagtaac ggcagagagt tctttagacg
    36361 gattaatggt acgagacttc aattggccta aaaatatgct gctaatctct atacgcagag
    36421 gaaatcagga aatcttgact cacggtgata cgatcatgaa tgtaggagat attttggtta
    36481 tcttgactga tgaagcaaac ttgccaagaa tcaagcagga aatcgaggct aaatcttcta
    36541 tcacaaattt gaagcaatca ttttaacgaa catggaaaaa ctattttaca acaactattc
    36601 atctacacta cttgattcta tatgattcta taaaaaaaga agaggctggg aaaacccagc
    36661 ctcttctttt ttatttctat gcttctgcta ctgcaggagc ttttctcaat aaaccataaa
    36721 tcacaccaga tacgactgct ccgatagcaa taaacaccaa atacatgacc gggtgactca
    36781 gtaagaatac aacgaaaatc ccgccgtgag gagccagtaa tttgattccg aaagctccga
    36841 ctaatccgcc agttaaagca gaaccaataa taaaacttgg aatggcacga atcgggtcag
    36901 ctgctgcaaa tggaatcgat ccttccgtga caaacgatag tcccatcacg atgttcgtca
    36961 aaccagcatc ttgatctgtt ttgctgaatt tattcttaaa caaacgtgta gcaacgaaaa
    37021 ttgccaatgg aggtaccatt cctccagcca taacagaagc catcacgaca cttccgccag
    37081 ttgcaacagt cgtagctaaa gttgctgtac caaacacata agcggctttg ttgattggac
    37141 cgcctaaatc tgccgccatc attccagcta ataatgcacc aagtaaagca gcattcgaac
    37201 cactcatact tgtcaagaaa ccgttcaatg catcattgat tgctttcata gggacattga
    37261 ccagcaacat caagaaacca gtaatcagta agccaaacac tggataaaag aggatcgttt
    37321 taattccatc caatgatttt ggtaagttct tgaataattt acgtaaaaat acgatgacat
    37381 aaccagctaa gaatccacca atcagtgcac ctaagaatcc tgcaccgcct gtattagcta
    37441 atgcaccagc agcaaatcca acgatcaaac cagggcggtc accgatactt gaagcaataa
    37501 atcctgctaa taccggcagc atgaaaccaa atgcagcttg tccgatttgg ttgaaccagc
    37561 tagcagcttg attgtaattc cctagatttg ataattgatc atgtggcacg ccaataaatt
    37621 ggtcgatcat gaaagaaagc gcaatagcga ttcctccacc aataacgaaa ggtaacatgt
    37681 gtgatacgcc gttcatcaaa tctttgtaaa ttctttgtcc gatagtacca tccgcatttc
    37741 cttcttctga actgctttct tttccatcac catggaatac tggtgcagta ccactaaggg
    37801 ctagattgat caactcttct ggtttacgga taccatcact gacaggacga ttgatcagtt
    37861 cttttccgtc aaagcgattc atctctactt ttttgtctgc tgcaacgatt acaccttctg
    37921 cacgggcgat gtcttcagca gtcaatcggt ttttgattcc ttctgaaccg ttcgtttcga
    37981 ctttgatctc cacacccatt tctttcgctt ttttcttcaa agcatcttct gccatgtaag
    38041 tgtgagcgat ccctgttgga caagctgtaa cggcaacaac gaattttctt tctggatgag
    38101 cagaagcttc tgcagcttgt gtttcttgtt ctgctttttc ttctgcttct ttttgctctt
    38161 cagctgtttg gaacaagtct tggacttctt ctggtgtatt tgcttctttt aattttccta
    38221 cgaaatctgc atcaatcagc aatcgagata gtgctgctaa tgcttgcaaa tgtgtgtcgt
    38281 ttgcgccttc tggagctgcg atcatgaaaa acagataagt tggctgtccg tctaaggctt
    38341 cataatctac tcctttttgg ctttttgcga ataaaactgt tgcttcttta actgcactgt
    38401 ttttcgcgtg aggcatggcg attccatcac caagacctgt agaagtttgt gcttcgcggg
    38461 ctagaattcc ttctttatat gtttcgatat cggaaatccg gccggcatca aacatttttt
    38521 ggaccatttc atcgattgcg ccttttttat cagtcgcttg caaatccatg atcatcgctt
    38581 ctttgaccat taaatccttg atattcatga ttatcctcct tgaaactaat caatttttgt
    38641 gatttcaact tctggcagca actcatcaat caattcgcga gctgccaagt catcagaaaa
    38701 agctgttgcg cttccgcaag caactcccca tttaaaggct tcgattggat ctttcgtacg
    38761 gctgtaagcc ccaataaagc cagcaatcat tgaatcccct gcacctactg aatttttcaa
    38821 tgggcgaact aatacattcg ttttataagt tccttgttcc gtgaagagca aagcaccgtc
    38881 tccagccatt gagatcagca cattctgtgc cccttcagct agtaattttt tcccatacgg
    38941 aatgatttct tctacagatt ggaaggttgt gttgtaaagc tcagctaatt cgtgattgtt
    39001 tggtttgatc aacaacggtt tcttaacaag tgcatttttt aggtcatcac ctgttgtatc
    39061 aatcacgaaa tctgcacctt tttctcgaat gatatcaatc agttcttgat aaaatccttt
    39121 tcgcaggcta gctggcgcgc ttcctgacaa tacgacgata tctccttgtc ctactgttcc
    39181 taatgcttgc ttcaactcat cgatttcttc atctgaaata gatgggccta agccattgat
    39241 ctcagtttca gtgtctgatt ttagtttgat attgattctt gtatcttctt ttacggttgt
    39301 gaacaaagac tggatttgtt ctttttcaag ccacttcgaa ataaattcgc cagtgaaccc
    39361 tcctaaaaac cctagtgctg tagattgaga atcgatccgt ttcaaaatac gggaaacatt
    39421 gatccctttt cctccaggta acttgaagtc ttccttcatt cggttcaaat cgcctagttt
    39481 taacccatct acgcgtacga tataatcaat tgatggatta agtgttactg tgtaaatcat
    39541 tgtattgcct ccttaattgt tgtcttttca cggaattcat taacgaattc taatggacaa
    39601 cgctcggtta tgatcgtggc tgcttccaaa ggtgcaacag aggtgaagga caactggttg
    39661 aatttggtat gatcaatcaa tacaaatgtc tcttcggaat ttttcatggc taaacgttta
    39721 acagctgcct cttctggatc cggagtcgtg aatccttgtt caagatgggc accgttcatt
    39781 cccataaatg ctttattaaa atgaaaattt tttagttgtt ctatcgcatt agatcctaaa
    39841 acagcattag ttgccagctt tatcgttcca cctaaaataa tggtagcaag ctccaaatca
    39901 actaaatttg ccgcatgttg aacggaattt gtcactactg tgatctgttt tccttgcaga
    39961 aaaggaatca tttctaacgt agtagagcca gcgtctaaat aaatgacatc ttcgtcatta
    40021 atggtttcag ctgccgattt ggcgatttgc tgtttttcgt gaacgttttt gagtgatttt
    40081 tcactcatat tctgctcaaa attcaataat tgctctttct tagctccgcc atgaactctt
    40141 tctagaaacc cactattctc cagctcttgt aaatcacgtc ttatcgttga ttcagaagca
    40201 ttcaaaaggt ttacaagatc ttgtgatttc acgattttct gctgttcaag caagtctagg
    40261 attttttgtc tgcgttcctc tgtaagcatt ttcacgatca cctcaataca aataatagca
    40321 tatttttttt ctactagcaa tataaatcag tcaaaaacgt tcataaaagt tataaatcag
    40381 tcaaaaaaaa aataaaaaac cagtcagaaa gactggtttt ttattttttt gtctatcatc
    40441 aagccgtttt atcgtttttt attttcgctt cttgtttttt tcatataaac agaagcaggt
    40501 tttctttcct tatgaagaag ataataataa agtcccatcc cgccaaacat gaccaaaaaa
    40561 ccgatgactg atcgaatagg aaaattagga acaaaaaaga gacagacgga taataatgat
    40621 gccggaacca ttattcgata atagagttgt ttatccattt tcttcacctc atattttttg
    40681 taaagtaatc aattgttcta attctcggta atctaaaaat tgcacacgca gatgctgctc
    40741 acctttcttg acatcgaatt ggatacttcg ccgctttttc tttattcgtt tgaaggtaac
    40801 atcagagagt ctatgtttga tttcctttaa cggtaattct gacagggatt ctgaaggaac
    40861 atagattctt aaggataaaa gatactcttg atatttttca tctgttacct ctacttctag
    40921 atattcccta atataataac aataagcttc gattccatta tccaaagcaa cagtcggatc
    40981 gataacttta tagcacatca tgcgtgatcg attctcgtca acccaaggaa aatacgcctg
    41041 agcatttagt gttccgcgaa cacgcctttc tttataatct ccttgttgga tatcataact
    41101 ttttgttaca aaaaatgaag cattcttttt aggaacaggt tgcatttgtg cattctgata
    41161 ctggacagat tggatcgtat ggtcagtgta aatgtattca acgaatggcg gtttcaataa
    41221 accgacacca ttgtcccaag caagatgatc aaacccaagc tggtccctaa aataggattg
    41281 aaacatttga ttgtttggct tttgccagtt aaaggggtct cgataaagct ggatacatat
    41341 gacttcgttt tctaattcct tcactttttc tacttgttgg atgtcgagaa aaatcggttt
    41401 agggatgatt tgatgataat agcgactata atacatttcc caacacgaag tgaaacataa
    41461 accttggtaa aaaagatcgt atcctggaaa ttggttgcaa tcgactacta cttccccgtt
    41521 ttgttgtttc attttaggta aatgttcgat ttcatccttt ttctcaaaca atagccgttg
    41581 agagatctgt tttgtattgt gatagataaa ctcgctataa gctcgcatat aggcgaacac
    41641 cccatgagca atcatttttt tctgaatgaa gtctcgaatc aaaaattgct tttctttgaa
    41701 aacagagata gacaatacat tttttatcaa taattgatta tcttccaagc gaaaactaca
    41761 ttcgttctcg tcatttccta caactaacca actttcgtct cttaccgatg ctagcattat
    41821 tttttcttga agctttcgaa gatcatacgg atccgcttgt tgtgtgaaag agcggataaa
    41881 tgttggctgg atcaacagac gtgtcaaaat agcccaatcg cttatttcct gttttttttc
    41941 ttcttcattt aagtctgtga gctggatagt catcaataat ttatcttcgc caatcatttt
    42001 ctcacctctt tcaaatcttt ttataattta attataacca atgataaaga aaaactaaaa
    42061 ctttatgtct tagaaaccaa tttggacgat tacatgatag actaagtaaa aagaaacaaa
    42121 gaggtgttta caatggggtt gaaatttgaa cgtacagata atttagataa acttttcgaa
    42181 tcatttgctg tcgatccgga aaaaacagtt gcaccaaaag aagagatcga tcgatttcta
    42241 aaaccacagc cacaaaatga aaagaaaaaa gacaagaaac aaaagtaagt taagctcaaa
    42301 aaaagactta ccagccaaac ttttatgaaa gagtttgact ggtaagtctt ttttattctt
    42361 tttatgattc aattgcttgt tcgattgctg cccacgcttc ttcttttccc tgttttgtaa
    42421 cggaagaaaa gagaataaac gtgtcattgc gatcaaaatc caacgctttt ttgatttcgc
    42481 tctcatgctt gttccattta ccacgaggga tcttatctgc cttagtggca acaacaatta
    42541 cagggatatc ataatacttc aaaaattgat acatctggat atcttcttga gaaggcttat
    42601 gacgcaaatc tactaaagaa acgacagcac gtagctggtc tctagtcgtt aaatatgtct
    42661 cgatcatctt tccccatttt gctcgctcag ttttggatac ttttgcatac ccatatcccg
    42721 gaacatcgac aaaatgtaat gcgttctcga tcaaataaaa attcaatgtc tgggtctttc
    42781 ctggcttacc ggaagtacgg gctaaattct ttcgatcaat caatgtgttg ataaaagaag
    42841 atttccctac atttgatcgt cctgccagag ctatttctgg caattgtgta tctggatatt
    42901 gttttggtgc cactgcactt atgataattt cagcttgatg gactttcatg gtctctctcc
    42961 ttaacaaggt gatgaataaa gcaagcggct gtcgcaaaag ccaaaatgac ttttacggca
    43021 gcctcttttc atcaacccgc ttttttctct gaatctttat agattactgt tggctcacca
    43081 gacaattcag ctgcttcttt tgtgataatc actttttcga tgttttcatt tgaagggata
    43141 tcaaacatca catccatcat gatttcttca atgatggaac gaagtcctcg agcaccggta
    43201 ttgcgttcga ttgctttttt cgcaatggct ctcaacgctt caggttcgaa ttcaagttcg
    43261 gtattatcta gagaaagaag tttttgatat tgtttgacta gcgcattttt cggctcagtc
    43321 aagatccgta ccagatcatc tgttgtcaat ttctctaaag ctgccataac aggtaatcgt
    43381 ccgataaact cagggatcaa tccgaatttt agtaaatctt caggaatgat atgctgcatg
    43441 acactttcgt cttcagaaag tttttggttt tgtgtaccaa atccaatcgt cttttcgccc
    43501 atacgattct tcacaatcgt ttcgatgcca tcaaatgcac cacctacgat gaacaatatg
    43561 ttcgtcgtat caatctgaat aaattcttga tgtggatgtt tacgtccgcc ctgaggtggc
    43621 acacttgcaa cagttccttc tagaattttc agcaaggctt gttggacacc ttcgccggaa
    43681 acgtcacgag taatagatac attttcgctt ttacgcgcaa ttttatcgat ctcatcaata
    43741 tagatgatcc ctttttctgc aagttccaca ttgtaatcag cagattgaag caatttaagc
    43801 aaaatgtttt ctacatcttc ccccacataa ccagcttctg tcaaacttgt cgcatctgcg
    43861 attgcaaaag gtacattcaa tgtttttgct aatgtttgtg ctaaaaatgt tttaccagac
    43921 cctgtaggtc cgatcaagca gatattgctt ttttgcagtt caacatcatc tgtgttttct
    43981 tgtgcttgct gattgacacg tttatagtga ttgtatacgg caacagctaa cgagcgtttt
    44041 gcgcgatctt gtccaatcac gtagttattc aatacttcaa gaatttctag tggtttaggg
    44101 acttcagtga attcgcgtac agcttcttca taaaattctt catcaatgat ttctttacat
    44161 aggtcgatac attcattaca gatgtagacc cctggaccag ctacgatctt tttcacttct
    44221 tcttgggttt tcccgcaaaa agaacagcgt acggtctcat tgctacttgg attgtcatac
    44281 atggtcttca ccccttaact tgcaaaaagt atgcaagaaa ctatcaaaga ctttacagtc
    44341 ttttactatc ataacatagt ttagtaaaaa gaaaaagtgt ttaacaaagg atcaactgac
    44401 ctataccttt gcatttttta ctcttaacaa aaaataagga actgtgacaa aatcgtgtca
    44461 cagtccctta tttcgaataa acaattttct agaagtagct tcttcagaaa tgatttatta
    44521 cttgatttct attctttcgc ttctgtcgct gtacctgtga tcaattcgat tgcttttttc
    44581 attgtcacat catgttccag catatcttct gtcaacacgc gtttgatttg ttcgattggc
    44641 atgttgtatg tttctgataa ttcttggatt tctgcattga tttcttcttc tgttgcttga
    44701 agattttcag cagctgcaat tgcttcgatc acaagatttg ttcttgtgcg catttctgct
    44761 tcaccttcga attgtttatg caagtcttct tctgtagaac cagttaattg atagtacatt
    44821 tcaggagaga taccttgacg ttgcatgttg ttcaagaatt cgtccattga acgatgtact
    44881 tcatcatgaa ccatcacgtg tggcaattca acgatttctg cgttttcaac agccatacgg
    44941 atcgctgctt catcttttgc atcgtccgct gctttttctt ttgtttctac taattctttg
    45001 cgatattttt ctttcaattc gtctaatgtt gatacttcgt catcaacatc ttttgcgaat
    45061 tcgtcatcta attcaggcac ttcttttgct tttacttcat ggattgtcac tttgaagact
    45121 gcttcttttc cagcaagatc ttctgcttga taatcttctg ggaaagttac atttacatct
    45181 agactttcac ctgatttatg accaactaat tgatcttcaa aacctggaat gaatgagcct
    45241 gaacctaatt caagtgagta attttcgcct tttccgccat caaacgcttc gccatctaca
    45301 aagccttcga agtcgattac tactgtatcg ccattttcag ctgcagcatc ttctttgata
    45361 actaattcag cttgtgcttc ttgttcacgt ttgatacgtg catccacgtc agcatctgtt
    45421 acttcacggt cttgtttttc aacaactaga tctttatatt gtcctaattt tacttctggt
    45481 ttcactgtta cttcagcagt gattacccag tcttgacctt tttccatgct ttcaacatcg
    45541 atttttggtt gagaaacagg atcgatgcca gcttctttga cagcagcttc ataagcttct
    45601 ggtaatacag cattcaatgc atcttcatat aaagcttctt caccgtacat acggttgaat
    45661 acttgacgag aaactttccc tttacggaat ccaggtacgt tcaagctttt tttcacttta
    45721 ttaaacgcag ttgttaatcc tttttggatt tcgtcttgcg caatcgtaaa ttttaaaaca
    45781 ccatcattcg tgcctttttt ttcaaatgtt gcagtcattt aattccctcc gagtttagaa
    45841 attcatttta tacatcaaaa acgtttcatg cataccttta aatagtacac taaccctttc
    45901 aaattgtaaa catattgaat ggatagaatc cttgtttttc ataacatttt tatctttttc
    45961 actgataaaa tctaatggaa gaagatcatt tgctgcaaaa ttttttctct ttttt
//
LOCUS       DAMEET010000007        45667 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.007, whole genome shotgun sequence.
ACCESSION   DAMEET010000007 DAMEET010000000
VERSION     DAMEET010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 45667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.007"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            4..243
                     /locus_tag="PB780_000365"
     CDS             4..243
                     /locus_tag="PB780_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732441.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            310..519
                     /locus_tag="PB780_000366"
     CDS             310..519
                     /locus_tag="PB780_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732442.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            579..1250
                     /locus_tag="PB780_000367"
     CDS             579..1250
                     /locus_tag="PB780_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HDE0732443.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1303..3279
                     /locus_tag="PB780_000368"
     CDS             1303..3279
                     /locus_tag="PB780_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDE0732444.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            3292..4047
                     /locus_tag="PB780_000369"
     CDS             3292..4047
                     /locus_tag="PB780_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDE0732445.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            4044..4868
                     /locus_tag="PB780_000370"
     CDS             4044..4868
                     /locus_tag="PB780_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732446.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            5169..7268
                     /locus_tag="PB780_000371"
     CDS             5169..7268
                     /locus_tag="PB780_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDE0732447.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVED
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(7306..9363)
                     /locus_tag="PB780_000372"
     CDS             complement(7306..9363)
                     /locus_tag="PB780_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HDE0732448.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(9477..9689)
                     /locus_tag="PB780_000373"
     CDS             complement(9477..9689)
                     /locus_tag="PB780_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732449.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            9876..10343
                     /locus_tag="PB780_000374"
     CDS             9876..10343
                     /locus_tag="PB780_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732450.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            10340..10582
                     /locus_tag="PB780_000375"
     CDS             10340..10582
                     /locus_tag="PB780_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HDE0732451.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            10600..10902
                     /locus_tag="PB780_000376"
     CDS             10600..10902
                     /locus_tag="PB780_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732452.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            10974..13028
                     /locus_tag="PB780_000377"
     CDS             10974..13028
                     /locus_tag="PB780_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732453.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            13028..15640
                     /locus_tag="PB780_000378"
     CDS             13028..15640
                     /locus_tag="PB780_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HDE0732454.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            15637..18168
                     /locus_tag="PB780_000379"
     CDS             15637..18168
                     /locus_tag="PB780_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732455.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            18220..18552
                     /locus_tag="PB780_000380"
     CDS             18220..18552
                     /locus_tag="PB780_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732456.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            18553..19146
                     /locus_tag="PB780_000381"
     CDS             18553..19146
                     /locus_tag="PB780_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732457.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            19309..21003
                     /locus_tag="PB780_000382"
     CDS             19309..21003
                     /locus_tag="PB780_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDE0732458.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            21691..23568
                     /gene="ltrA"
                     /locus_tag="PB780_000383"
     CDS             21691..23568
                     /gene="ltrA"
                     /locus_tag="PB780_000383"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDE0732459.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            23699..24076
                     /locus_tag="PB780_000384"
                     /pseudo
     CDS             23699..24076
                     /locus_tag="PB780_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            24076..25281
                     /locus_tag="PB780_000385"
     CDS             24076..25281
                     /locus_tag="PB780_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDE0732460.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            25297..25941
                     /locus_tag="PB780_000386"
     CDS             25297..25941
                     /locus_tag="PB780_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732461.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            25952..26758
                     /locus_tag="PB780_000387"
     CDS             25952..26758
                     /locus_tag="PB780_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732462.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            26780..27010
                     /locus_tag="PB780_000388"
     CDS             26780..27010
                     /locus_tag="PB780_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732463.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            27556..27831
                     /locus_tag="PB780_000389"
     CDS             27556..27831
                     /locus_tag="PB780_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732464.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            27873..28295
                     /locus_tag="PB780_000390"
     CDS             27873..28295
                     /locus_tag="PB780_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732465.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            28314..29906
                     /locus_tag="PB780_000391"
     CDS             28314..29906
                     /locus_tag="PB780_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732466.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            29918..30253
                     /locus_tag="PB780_000392"
     CDS             29918..30253
                     /locus_tag="PB780_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732467.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            30400..30594
                     /locus_tag="PB780_000393"
     CDS             30400..30594
                     /locus_tag="PB780_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732468.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            30626..32998
                     /locus_tag="PB780_000394"
     CDS             30626..32998
                     /locus_tag="PB780_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732469.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            33059..34519
                     /locus_tag="PB780_000395"
     CDS             33059..34519
                     /locus_tag="PB780_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDE0732470.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            34530..36734
                     /gene="topB"
                     /locus_tag="PB780_000396"
     CDS             34530..36734
                     /gene="topB"
                     /locus_tag="PB780_000396"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDE0732471.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            36750..36899
                     /locus_tag="PB780_000397"
     CDS             36750..36899
                     /locus_tag="PB780_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732472.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            36973..37566
                     /locus_tag="PB780_000398"
     CDS             36973..37566
                     /locus_tag="PB780_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HDE0732473.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            37582..38481
                     /locus_tag="PB780_000399"
     CDS             37582..38481
                     /locus_tag="PB780_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HDE0732474.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            38484..38969
                     /gene="ssb"
                     /locus_tag="PB780_000400"
     CDS             38484..38969
                     /gene="ssb"
                     /locus_tag="PB780_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDE0732475.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            39335..39595
                     /locus_tag="PB780_000401"
     CDS             39335..39595
                     /locus_tag="PB780_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732476.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            40036..40242
                     /locus_tag="PB780_000402"
     CDS             40036..40242
                     /locus_tag="PB780_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HDE0732477.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            40242..40493
                     /locus_tag="PB780_000403"
     CDS             40242..40493
                     /locus_tag="PB780_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732478.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            40508..40945
                     /locus_tag="PB780_000404"
     CDS             40508..40945
                     /locus_tag="PB780_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732479.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            40938..41645
                     /locus_tag="PB780_000405"
     CDS             40938..41645
                     /locus_tag="PB780_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732480.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            42026..42205
                     /locus_tag="PB780_000406"
     CDS             42026..42205
                     /locus_tag="PB780_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732481.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            42546..42809
                     /locus_tag="PB780_000407"
     CDS             42546..42809
                     /locus_tag="PB780_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HDE0732482.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            42803..43153
                     /locus_tag="PB780_000408"
     CDS             42803..43153
                     /locus_tag="PB780_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDE0732483.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(43177..43791)
                     /locus_tag="PB780_000409"
     CDS             complement(43177..43791)
                     /locus_tag="PB780_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDE0732484.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            44105..44527
                     /locus_tag="PB780_000410"
     CDS             44105..44527
                     /locus_tag="PB780_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732485.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            44537..44740
                     /locus_tag="PB780_000411"
     CDS             44537..44740
                     /locus_tag="PB780_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732486.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            44951..45535
                     /locus_tag="PB780_000412"
     CDS             44951..45535
                     /locus_tag="PB780_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDE0732487.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT        16741 a         7205 c         9260 g        12461 t
ORIGIN      
        1 tagatggcgc gaccagtttt agaggcgatg gatatggata ctttgacaaa aggaaggtat
       61 gtacagctgt tagctcgtgg ttattcacct aaagaagtat tcaaagcctt atctaaagga
      121 acagatatgg aaaaagaaag attgcgaaaa gaattcgatt attggagaac ccataatgga
      181 atcaaggatt tgaaaccagc acggccagta attaaacgaa agaaaaagat aacccaagac
      241 tgaagtgtag agcgtatgtg atcataggct tttttatttt tgcaaaaaac aaggaaggta
      301 ggaaaactca tgaattatga agaaatcgaa aacagaaaaa aagtgtcgaa agaaatggaa
      361 gaaaagttac taaaaacgat gaagcaaaaa caccttaaaa gattatctgt cgcgcaatat
      421 atcaatgata tgcagttaac aggaaaagag aaagcttgtt tgttaggttc aatgaaaaac
      481 tttgaacagt tgagacgaac ttatagaatt catttttaaa actttttcta tggaaatgag
      541 aaaaagtttt tttgttttgt ctgaaaggag gcagatggat gaaaaaatgg ttatttggtt
      601 ttttaggagt cactttaatc gtcttttgca gtgtgtttgg ctatgttagc taccaaaaac
      661 atgaaggaga ggtgtttaag caaaatatag aaaaaaagat gcccgtagat caaatcaatg
      721 cacatgcaaa gagttataag gaggatgcaa cgaacgtcaa taatgatatg tctttggggc
      781 aaatgttgtc catacaaaaa gaagcaatcg aaatgggcgt caataaacaa gtatttgcac
      841 aaattcagat tcctgcactt ggtttagcat tgccaatttt taaaggggca aatcaataca
      901 ctttgagtct tggagcggct acttattttt atgaagatgc cgaaatgggc aaaggaaact
      961 atgtcctagc aggtcataac atggaaatgc caggtgtttt attttctgat attcaaaagt
     1021 tgtcattagg ggaggtgatg gacttagtta gtaacgatgg cgtctatcgt tacaaggtaa
     1081 cgcgtaaatt tatcgtacct gggtacttta aattaatcga tggcgttcct gaagagaact
     1141 ctttcttgtc tttgccaaag aaaggagaga aacccttatt aacgttgttt acttgtgttt
     1201 atacgtcaca aggaaaagaa agatacgtgg tacagggaga gctacaataa caaggtttta
     1261 atcaacgcga gtcgttgaaa tatgaaatga ggagagtaaa aaatggcaat aaatcggtat
     1321 gtaaaaaaag ctggaatgct tgttggaatg ttaggaatca ttgcaggctg ctttcagggg
     1381 tatcaagtac atgcagaaga tgtcacgcaa aaaacaccac cagagaaggt caatatcacg
     1441 gttcataaat taatgtacga tcaaggaacg caattgaatg ttgatattga cgggatcaaa
     1501 aacgatggtt atacgcacga tcaatatcct acaggtgtaa cgaaatacaa caaggcagat
     1561 tatggtgatg ttgagtttac gttaggaaat attacggatc aagtattacc gagagaagat
     1621 tctgatctaa ccaacgcgaa agtggacgag attgtacaag atgtagaaga caagggatct
     1681 aacagtgagt acgtcaagaa tgcaaccaac aaaattactt cagctgtaga tgaaaatggt
     1741 gagatcactt ttactgatca accagcctat gtcaattcca aagggaatgt atatgtggtt
     1801 tatgaaagta aatccgctgc tggattagtc acacaaaaag caaaaccaat ggttgtgatt
     1861 gcgccaatga cagataaaac aggatcagga tttttaaaaa atattcattt gtatcctaag
     1921 aacattgttt ctaagttgag ttttgaatta acgaaatttg gtgacgatgg cgcagaccaa
     1981 agcaaacaaa cccttttaaa aggggcaaaa tttgagttat acaagggcga acctggaaaa
     2041 ggaacaaaat tgggtgatct tgtatcggat gatcaaggaa agctgactgc gactgatttg
     2101 acgttaggaa aatactattt tgtggaagtt ccttcggaag ttgtagtggg atcagatcaa
     2161 gaaccaacag cagatcaata tttattaggc gcagacgctc gcaacgatgc gcacaataaa
     2221 ttaacctttg aaattacgaa tgatggcgtg acaagtgact taaaagcttc atacgtaaac
     2281 tataaagcac cagtgatcga taaaacagta acaaatggaa caggacaaga acattctttc
     2341 caaattggtg acgcagtaaa ttatcaagga acgattcata ttccaacaga cattgcaggt
     2401 ggcgcagacg gtatcacagt aaacggtgtg aaaagcgaaa cttctcctta cagtgtattt
     2461 aaatggggag atacagcagg taaaggactt tcttatgttg ctgaaaaagc agatattaaa
     2521 gtaacaaata atgatggttc agtcgtatta aaagaaaata ctgactataa aatcgaaaat
     2581 tctgatagtg gttttgtgat cgattttatc gtaaataatg gacaagtaag tgatacagtt
     2641 gcgaatttac atggtcaaga tctaaaaatg acttataaca tgtttgtgaa tgatagtgct
     2701 gaaatcgcaa atccattaac aaatagcgtg gatttcgttt ataacaataa tccatttaat
     2761 caggaagaac accacgaaaa aacgaaagct gacgttgtga cttatggcgc aaaattctta
     2821 aaagtcgata gcggcctatt tggaacaggt atcaaagcaa caccgcttga aggcgcagaa
     2881 tttgcagcta agaatgcaga aggtaagtac tacggtggac ttactgatac agataaagat
     2941 ggtgtcaaag aagcagtttg ggtagacgat gtagcaaatg cagcaatcct aaaatcagat
     3001 aaagagggtc attttgaaat cactggttta actgaaggag attactcact ggaagaaaca
     3061 aaagctcctg aaaactatca aaaattaaca aaagagatct ctttcaaagt agacaaagat
     3121 tcatacaaag aagaaaaccg aatcacgatc aaaaataatc aaaaagcttc cgtaccaatg
     3181 actggtagca acggtttcca atcttacgta ttgatctctt gcttgttgct aggagcaggg
     3241 ggactttctg cagttgttta ctttaagaag aaagcctaga aggatcagct aatgattatt
     3301 cgtaagcccc caaaaagaag agtcatgcac aaactgatga tatttttttg gttgctgtcg
     3361 gcagtagcag tctatttaat tccagttgtg gaaacaaatg tagcgaaatt tcaacaacag
     3421 caaagaattt tagccattga aaatcttgaa aaaacagatt cttctttgga tgttgcgaaa
     3481 atagagttag gcgatcctgt tggtgttctt acaattccaa gtatttcttt aaaactacca
     3541 atttatgacg gaacttccga caaaatttta gagaatggtg tagggattac ggaaggaact
     3601 ggcgatatta caggaggaaa cggtaaaaat cctttaatcg cggggcacag tggactttat
     3661 aaagataatc ttttcgatga tttgccttca gtaaaaaaag ggcaaaaatt ctttattaag
     3721 gtaaatgaag aacaacatgc ctatcaaatt tatcgaattg aagaagttaa agcagaagaa
     3781 ttacaaaaaa attatttaga ttatcttatg cctgaaaaag accaagatag gatcacgtta
     3841 atgacatgca caccaaaagg tgtcaactca caccgctttt tagtgtatgg tgaacgagtt
     3901 tccttcagta aagaagattt aaaactacag aaaaaagcaa aaaataggaa atcaatttca
     3961 ttggattggc gattagcact gctattcgct tttttttgcg ttcctattat ttttatttat
     4021 aaaaaaataa ggaggagatc ggaatgaata agagaaaatg gctgtttgtt tgtgtagcag
     4081 ctctcttttc tactgtagct tattcgcaac tgtctttcgc tgacagcagt gatagtagta
     4141 ccactcaaac aatagataat tcaattactg caccaagcga aacggacagt tcggagaata
     4201 ctacagaaga aacggcagat tcaaatataa gctttactga atcaacgaat ccaacgtcag
     4261 aaagttcttc agagacttca gaaaagccgc aagacgtaga agatacgcaa gtgatcaagc
     4321 aagccattca tattcaaaaa attatttctt cgaatcaaat caacaatcaa ggcagtcaag
     4381 tagcccagca agaaggtatc aatggcgcga agtttattgt ctacgatatc acgaatatct
     4441 tagaagaaat gtcacaagat gataatgaga aaaatcaatc tgtagaaata attgagtcaa
     4501 aattgaaaga tcgagcaaaa aaactttcat acgaccagtt gaaaaaagtg gctgaaggag
     4561 aaaccaaaac gattgatgat caggaaggcg tgattgaatt ttccattgaa gtacctgcaa
     4621 acaaaaaaca agcatactat atcgttaatg aatcaagccc tgaaaacatt tcaaacagtg
     4681 aagatattgt tttattgaca cctgtttcaa atgaaaaagg tgaatttttg acggatgttt
     4741 ggttataccc taaatcagag aaatcagagc cgaaagaaga aattaaaaaa gttgtgtcaa
     4801 ctggtgtaaa gaagaacttt tttgaaaatt gttgggaatt ttttgctcaa ttattttttg
     4861 ataattagga tgaaatgatg aaaataataa gaatattagt ttttaatcta tcctttttag
     4921 ccgcaacaat attgtgttgc ggtttaattt tttctcaatt atcaatttat ccaagatatc
     4981 aacgattgtt cattcatttt ctaaacattc aatgtgcttc catgccatta gaggtgatta
     5041 aaaagctctt gatcaatcca atttttatgg ttagtttggt tcttcttaca ggattggtaa
     5101 cgtgggtatt aggaaaaaaa gaatgaaaag cttacgatcg tttaaaaaaa tattagatgg
     5161 agggcattat ggaaaataaa attcattgca ctacgggaag agtaattact ttagtgttgt
     5221 ttttgttcct tcttcttttt gcgaggggaa ctgcagtcca ggccttagaa aaagttgatc
     5281 cgagtcaaat caaagaaaat aataatctgg tgctttatca tgatagtgat tcattgagaa
     5341 atatcgaagt aactgggatc aatggattta agatactaga tgagtattca actaaaaaac
     5401 aaaaagtact tgcttggcca aaagtcaaag atttgaaaag tacgtcaaaa atcgttgccc
     5461 attatcaaaa agtaggtact tatctaggaa aagaaattga ttgtgacgtg actttttcta
     5521 attttgtagc gcacgctggg gttaaccaag acggattagc taaaacaatt aatggcaaaa
     5581 agtattataa ttatttgctc tttgataaaa atatttatcg aggatttttc atgcagtcaa
     5641 tcaatgtcat gaatgcaaaa ctagaattta agtatgcagg aacaaaaaca aaagtagctt
     5701 tgagtgaacc acagccagaa attcaatcaa aaatgagatc gatgagagca gctggtattg
     5761 atttaaacga tacatttcta tccatttaca gtttaaatgg acagtatcag caacgtgtgt
     5821 cttggtataa tgacaataat gaatctgtcg gtgaacgtaa tattgatatg agagaatttg
     5881 ttgggtatga aaaaatgggt agcttacctt attttgtcac aacagataca gcatgtgcag
     5941 aatacaaagc tcctgcgtta tcaacaaaca atttaacttc aaaagtagtg ggaggacgtg
     6001 cagaaaaggc ttatagctcg aatgatcatt tcaccgatgt tgtaggagct gatacttatc
     6061 acagaagtgg tgtaacgtat acgcttcaag gcgcttcccc aacattcatg attggcgcaa
     6121 atacgaatag tatgatgttt agctttgata ctgcattgct atggacacca caaccatcga
     6181 agcctacaaa agaagtgttt aacaaagcta atactgaaga ggcagcacac aatattgaca
     6241 aaaaagtgat tccacaagga tcagatgttt actatcatat tcaccaaaag tttgatgcat
     6301 taacagtcaa cacaatgaac aaatacaaat catttaaaat cactgatacc tttgacagca
     6361 aaaattttga tatggtatcg gatgggaaaa actatgatgg cgcattgtgg tatgttgaag
     6421 cgaatggtac ttggaaaaaa gctgatacag gaggaaagtt aactgtatca ggtaatacag
     6481 tcacttatac tgcttctgac agctttttag ctaacgctgc aaattacggt agggattata
     6541 ttttgcagct ccatatgaga gcaaaaaatg atgcaaaagg aattgcacca aatgtggcta
     6601 ctgtaacgtt taacaatgat ccgaacaaag gaacacaaaa aacaaataca gtagaaaact
     6661 atttctctct taaaccaaat aaaaaagtgg aacaagatgg gaaagatgtt gatggtcgaa
     6721 atgatggtaa aaaaggaacg cctacagctc ctttaaatgc aggatctgaa gtacaatatc
     6781 tagtcacaca aaaatggcat acaaaaggtg tagacattgc ctcagatcat tacaagcaat
     6841 ttagtattca agatccagtg gaagatcgtt tgacgtataa agaaggttct gcgcgagtga
     6901 tagatatgag tacaggaaaa gatattactt cagaaggtac actaacctat gatagcaatt
     6961 caagaacgct gaagtgggaa gcttccgctg atttcttaag taaaaatctt ttagatggac
     7021 gagaaattca gctgatattt acagctaaaa ctcctttgaa tgaagaattg gatatcgaca
     7081 accaagcaaa agtttcagta gaaagtattg agaaagcaac taatgtagta acgattgggg
     7141 taaaacctaa cttacctcag atcattgtgc caaaaactgg atcgaatcat ttagtgctaa
     7201 ctgttattgg aagtatatta tttcttactt tagcatcatt gggctatgta gttttaaaac
     7261 tcaaataaat ataaaaacaa ggctgagaat cagccttgtt ttcttttatt tgaagattgg
     7321 attcgcacta ataaatacat tatctacgcc aattctacca gcagagtcaa ctacaaaatt
     7381 atgacctaat ccgagaatac tttggttatt ataaagtaaa tttgcattat gggcagggct
     7441 atttttccat cctaccatgg catcgatagc accatcgtct gataaaaggt catcaatata
     7501 cagcccagtt tgacttatgt tttctccctt agcaccgtag cagcctaagc tttccaataa
     7561 atcttggaaa gtaccaccac cagggcgata gtgactgaag ctgacttgta attctttagc
     7621 tcgaatatct gcagcctgtt gaacaatatc attgaattgc attggggcaa caccattttg
     7681 ttggcgataa tcatttaacc aagcaagttc tttttgacgc ataaagttac gtaattcgtc
     7741 taatgaaatc tttttatatt tgaatgtgat tgttttattt tgatcgacgt tattaatgga
     7801 tcgttggccg tttttatcca cggtaacggt aggatcgtca tttctgcaca agaaataatt
     7861 ttgatcagtc atgtttttcc aaggagtagc aaagctatcc ccttcgtata ctgtttttga
     7921 ttcactagag agaacagcgt tggtatcagc atttacatga ttaacagtca cattaaattt
     7981 acgagtgtag ttgaaaatat aattttgact ttcagtcaca ttgtttaagg ttactgaatt
     8041 tgttggttct acccgatcgt ctaaaacata tccttgaata gaaggagcag ttaaagtgac
     8101 actgtcaccg tgagtgacat tgactatctg ttctttcaaa atattaactc gtccttcgtt
     8161 tttgtagttg ttattgcaca acaaattgat ttgatgcact tgattataat tgaatgtgat
     8221 tgttgtatta cctgtgattg ttttgctttg agtatcatta ccaatcactt tgtagctaaa
     8281 attatcttta gcgccttcgc tatatttaaa tgcttttgcc ttagcagtgt aagtagaacc
     8341 agaagttact tttgcttgat ctgtagcaat cacttgacct gtttgattgt caatatggtt
     8401 aatagtaacc gtatattctt ttggtgtttc aggtttgtgt tgcaaccctt taaatgcagc
     8461 atcaagacta tttttagcat tgtttacttg tgtttgagaa accttagaat cgtctagtac
     8521 ttttttagca ttggataaag cagtctgaaa attattccaa gattcttctg taaaatctcc
     8581 tttagcagtt gattttactc ggttgtataa agcttgtaat tcagctttat tcacagtagg
     8641 aactggtttt ttctttaatc ctttgtaagc agaatctaat ttatttttgg cactatccac
     8701 ttgcttttga gtcgcttttg agtcatttaa cactttctta gcattgttta aagcggtttg
     8761 gaatgcgttc caggtattgt cggtatagtc accttttttc gttcccttaa cgctcgtata
     8821 aagagcttct agagccgatt tattcacttt gggtttagag gtgtcagcgc cataccaacc
     8881 gataccttcg tatttccagc catagtcttt ggttaaccat tgtacttctt tccaacttgc
     8941 agtgtagtga tgtgagccag attttacacc aggaaggaaa aggcggtaga tcggtttatt
     9001 atttttttcg gcagaacgcc aggcaggccc ttcataagtc cagccgtaat ttttagtcaa
     9061 ccatttcact tcatcccaac tagctgtgta atggtgatca cgaagacctt tattgtacaa
     9121 acgatagaca agatggccac cgctagtcgg tgcttcccag gcggggcctt cataatttcc
     9181 ccattttatc tttactagat ggtcacgttc ttttttgcta gaggtgtaaa aatgttcatt
     9241 attatttgga ttgtaaaggc gatacatgac gttagttgaa gcagcgtcaa cagatccagc
     9301 aagactaagt gaggcagcaa atgttccaat agctaaaaca gtttttggat taaataattt
     9361 cattttttca actcctaaat ttaattaaaa atgtttacat gattattata tatcggctaa
     9421 ccgatatagt caataaaaaa taagaaaagc cttatataat tttggctttt cttattttat
     9481 ttgttagttg aagtattaat atcaacgatt tcttcagcca atttatttaa tttcatagca
     9541 tttatgaatc tcatgttgtc tatactagag cgtttgttgg caaggtcact tactgtagtt
     9601 aagggtacgc cagattctat ggatatagaa cgaactgatt taccactatc aaacagcaac
     9661 cattcgattt tctttttatc agcgtacatt tcaatgctcc ttcttgtttt ttctttatta
     9721 tagctctttt ttgtaatgaa tcacaatatc aagagaaaaa ttttctatag aaaggtggat
     9781 ttttatgagt attcgttctc cgtgtatcca aatgaggttt gttgctactt tcgttatttg
     9841 atatactaaa aataaacaac gaaagaagga ataaaatgaa gaaaaagcaa ctatggtttg
     9901 tctctatgtt tgcaatattt tttattattt taagtggatg ttcaagtaat aagaaatcgg
     9961 aagctaaaga agataatagt ttattgcaac caggaactgt ttggaaagaa gaagtaggtg
    10021 gtcttattca taatttaaaa attatagatg atactacctg ggaatattct gaaagtgtat
    10081 ggcatccaga tcctgtacaa ataacagtta agaggcaaaa agactatgaa ggtttagaaa
    10141 gatacaaaat agtagattct gcaggcatta gggaatttat aaacaaacct gattcattat
    10201 ttattgttgt tccctatgag aaggatggcg taaaaaaaat aatttttctt ggatctagca
    10261 aggatgagaa gcgaacgaaa gagaaattaa ttcatgatgg atcagagtta aataaataca
    10321 aattacaaaa gactagtgag tgattgctag tctttttatt tatcctgaag gaggaatcac
    10381 agtggcaaaa gttaaaacac cattcgaaaa agccgcgtct aaaatcttag aacgccgtgg
    10441 tgtttctgaa atacagtgga aaaaagaagt tcttgaaaaa gcacaatttg acctattacg
    10501 aggaaaagat aaggacttag aagaatatat ctatcaacgt gaacgtgaaa aacttgtatt
    10561 cgatgaaatt attaaacatt aaaaggagcg aataatctta tgtttgaatc aattacaatg
    10621 aattttttaa ctcaaccgaa agtagtagct gcagttgata tttttagagc attacaaact
    10681 gccgcaacta gtttgaaaaa taatctgatg atttttgcgg ctgtagctgc cgttgcctcc
    10741 attgctcttg gaggtatttt cttcttcttt gggcgaagag gagctgaaaa cgggaagaca
    10801 cacgtaggtt atgtattgtt gggggtagga gttattttag ctgccgcttc tattgtagtt
    10861 tcaatgttct caatgtttgg acaaacccca caaggactat aaaaaaaaca agtaggagga
    10921 gtaaaaagta cgtggctact ttttactcct ttttttatga aaggagaata ttcatgtttt
    10981 ttacgatttc aggaaatgca aaattggata aagagttaat aagaaaagca acgaagaaag
    11041 tgggaaagcg tacttccttt ttaccgaaaa caattaaata ttctgtcatt gaaacggaag
    11101 aacagctaca tgtgttagga caagtgttag aacaagagaa tcaatcttct gttaaattga
    11161 ctctcacgtt acatgataaa gaaggaaaaa tagaaaaggc tggcacagaa tacgtttttg
    11221 gcccactgga ttatcaaatt tcagaaggtt tttatcctac attgatagat ttaatccgcc
    11281 ttgatgtgtt taaagatacg gatgaaacta tgagtgtgga agagtcttat cgaaacgaaa
    11341 aactgctaca agttttagaa gaagagttag agagcgacca aagaaaagtg aatcaagtga
    11401 gtgaagtcga agtaaatgaa gaagtaccat gggaacgacc aatctttcca aagaaggagt
    11461 caaaagaaaa agagacagtc tatgttgacg aacaagctga tattccgact cagaaatcga
    11521 tatttgagga agagaacgat gtgatgggtc aattggaggt agatcccttt gaagaaacaa
    11581 tggaggatga agagacggac gaagtagaag ttgatccagt tgaagctaat gaagagtcaa
    11641 catggactga aggggatttt caggaatata ttgaaccact tatcagtgaa gaagtagaaa
    11701 aaaacgaaac agagatacaa aaggcaactg tatttgatta tattttggat caatatccgc
    11761 cagagcatga atggatgaaa gaaaaaaaca aattattttt aaatgagctt tattcgaaat
    11821 atcaattacc ccaaacctat caagcatttt tagagagtaa agaagaatta atctcacagg
    11881 gtaaaggcac acttttagaa cagttagagt ctgtccaatc aacaaattgg catgagcttg
    11941 cacaagaaga tttattagcc acctttgaag aacgtcaaca agaaacagat caagaaatta
    12001 atgagtatgc gacggaacag tcgtctaatt gggagaaagt aaaagaggaa ctaaataaag
    12061 aagaacagcg agtgattgaa gaagaagtac aaaaagtcaa agagcggtac gaaaagaaac
    12121 gcgacaatgc ttattccttg tgtgtagaaa agatcaacaa ttttactaag acgaatcaag
    12181 aagcgttatc gaaagaaaaa gaaaacttgt taaaagaacg tattaaagaa cgaaaagaag
    12241 agttctaccg cggtttacgt acagataagc taaagattag caaagaatta aatgaacaac
    12301 tgaataagct atatcaaaca acgactgaag ttcttatgga aaaacacgaa cagatccagc
    12361 aagaattaca agtacaaatt ccaaaatgga aaaaagagta tgaaattcaa caacaagaat
    12421 tgaaagaaca agaagaaaaa caacggcaaa aagaaaaaga gcgtgaacaa ttagaaataa
    12481 gaaaacggga acaggatctt gcggaacaaa aaatggcgat agaaaaacaa cggttagagc
    12541 aagaaaagga aaaagaagca gctgctcgta aggaacgtca agaacaaatg gatacattgc
    12601 gtcaagcaca gttgaattat ctagcacaac ctcaaccagt agctgctgtt caaacgccag
    12661 tgaacaacca agcaaattta actccttcac cccagcgttc ctttaacgga tggatggtgg
    12721 gatgtattgc aagtttaagc ttgcttctag gtggaggaac agtatttgct tttaatcata
    12781 tcaatcaatc ttcacaggct gattctgtag ccacaacggc gtcaatcaag gaagaagccc
    12841 aggaacaagc aagaaaagag tatgaacaac aattagaaga attattgaat cgtaccaatc
    12901 agtcaacaac agagtcaagc atggaaaata gcaaagagag tgatcctagt aaggctaaag
    12961 aaggatcaag cgattcaact gtacactcaa acacttccga aagttcagaa tcaaatgagg
    13021 tgaaataatg cgtattagag aaacaacgag acgaaaagac aaacgattca atttagaaaa
    13081 gaataggtat ctttctcatt tctatcgcta tactccttca ggagaagagc taccagaaaa
    13141 acatgacaaa cgtatttctc aaaagatttt ttatagtggc ttagcttatc ttcctttaaa
    13201 ctatgttgtt acaagaatac cagaagcatt tcatcgattt gagattattc caggaagttt
    13261 acaaggagtg aatatttttc agttacaaga aatgccgcag ttattccatt ttgattcaac
    13321 tggtttttgg aatcctttga ccccaacaac aagtttaggc acacgtttag ttgtttctac
    13381 gatttgtgtt gttccagctg tacttatggg attgaaaatg gacatgtggt ttcgtccgct
    13441 agcagatgga caaaaagggg ataatcgatt aatgacctta aaagaaatcc aacagattta
    13501 tccgcaaata cctgaaacaa aaactacttt tccaggttat ggaggtattc caatcacaca
    13561 ttacaaaaaa tattggtaca ttcaaacaga tactgttaat acgtgtattg ttggtacctc
    13621 tcgatcaggg aaaggtcaga ttgaagtgtt agccaccatt gacaatttat ctcgtgcaga
    13681 aaaacaaagt tcaatggtcg ttaatgatcc aaaaacagaa ttatatgttg gtagtaaaga
    13741 tgaactagaa gctagagggt atgatgtata tgcctttaat atcaccgatc cactgcaatc
    13801 aatgagtgat aatccattgg cactcatcgt gaaatactgg aaacgaggag acatagatac
    13861 tgcaacacag ttaaccaata ctttcacaaa tacgatttat tatgatgcca acgcttcaga
    13921 taacaaatac tttaacgaaa acgcgcagaa agctgtcaat gcgcttattt ttgcgttgtt
    13981 agaacaagct gatcaaacag gagattactc caaattgaca ccgaataatc tcgttgagct
    14041 tctttctgag attggcggtt ttaattatta tccagatcca gacaatgaat tcaaacagtt
    14101 gaatgcctta gatgaattta tgaatcagtt gccacctgga aacattgcaa aaaaacaata
    14161 tggggcaacg aaaattggat ctgataaagc gaagggaaat attttatcaa cggcaatcac
    14221 gggattgaat ccatttactc taacgaaaat tgcaaagatg acttctcaat caacgattga
    14281 ttataaatcg gttgggtttc caaaatactt agatttgaaa ttacatgaaa gtcttttaaa
    14341 tcaacgagtc gtagtagaat tttatcataa gggaaagaaa tttcatgaag aacgtgtcaa
    14401 agtaggctat aaaggattta gtgaaatcaa ttttgattgt catttagttg caggtgatca
    14461 agtttgttta aaatacgatc atcaaggaaa agaatatgtg gcttggtatg aattttctca
    14521 aagagtattg aaagacgaag agggtaatat cgtttatcgg aaaaaacgag gagaaaccca
    14581 tttgccagaa ttagaaaaag aagccgttct aacattagat gaggaaaaaa gtaacttatt
    14641 agtagagtct attcggatgc actataccga taaaccaatg gctattttta tgcttacacc
    14701 tgattatgat ccatctaacc atatcattgt tagtattttt cttagtcagt tatataaaga
    14761 attatctacg caatgtgtta agactaaagg agataaatgt caccgcagga ttcattttat
    14821 tttagatgaa tttgggaata tgccaccaat ggataatatg gaaggaatca tgacggtcac
    14881 agctggccga aacatgttgt gggatctgtt tattcaatct tatcagcaac tttatgcgaa
    14941 gtatggagaa caaaacgcag cgattatcaa agaaaattgt cagacacata tttatattat
    15001 gtcgacagat gataatacca tcgaagaatt ttctaaaaaa gtggggaata agacggttga
    15061 acaagagaac agtaccatca attcattagg tatgaatact catatcaatc gcaatgttga
    15121 ttcagatcgt atcttaactc ctgaaagaat ttctactttt ttagaaggag aaacgattgt
    15181 actcgctcct ttaaaacgac aagatttgaa aggtcataaa atacggccgt atcctatttt
    15241 taatcatggg gaaacgaatc ttccttatgc ttatcaatat attgaagatt tcaatccatc
    15301 gtgggatatc aatgatatag acatacattg tacacatgcg gacttggatc ttaaggcttt
    15361 acaaattgat tatgattatt tcctaccaga tgaatatgct agaaatctct atcatggact
    15421 gcatgatcaa ttagaagaaa aaaacgaaga aaatatgttt tataaaatcg ttcaatcttt
    15481 tgagaatgag acggctaaaa aattgttaat tcaattttac gaagaacaag cagcagatcg
    15541 acttcaagaa tatgttgaaa agttgatcaa aaataaagaa atttctgaaa tgattgggaa
    15601 acggttgtta ttggaatctg aaaaggtggt gaaagaatga tcattggagg aattttttct
    15661 ggcatagccg actggtggtc aggcttattc ggagaagggg atgcaaaccc caaaaccgta
    15721 acgaaagtct tacaagatta tgggaattat ctgcagtatc aagatgtatt tagttggatt
    15781 tggaacactt ttctgtgggg attgattaaa ggattatata atctcaatac tttactagaa
    15841 aaaaccattt atcaaagctt tgatttaaaa gacttattga acgccgctgg ggtaaatgca
    15901 ctatttcaaa gtttgattag taaggtattg gctctttttt gtgtcgtcac attaatttat
    15961 ttaggcttga aattttttat gagtaaacac ccaccaaaag tcaaaaatat cttggttaat
    16021 ttatttatgg ccatgatctt gatttttggt ggtagttctt taatcgatga agggttaaat
    16081 atcagtaaaa gcttttacgg agacgtcact gcagcaaata aagcagataa aacgggatca
    16141 cctgcttttc aaattattca aaataatgtg atcgatgtca gtacgttact agcaactgac
    16201 ccgaataaag tagaatccct accaacagat aaaagaaatg ctttaacagc gaaaaatttt
    16261 aaaacagcga atatcaatgg gattattact cctgaacaag ctaaaggtat ggcaggtaat
    16321 gataaaagtg ggttatcaga tatacaaaag gatcggttaa aggcattaca ataccgattg
    16381 gaactagatg ataatgggaa agaaattcct gttcaaatta gtgacgaagg cttagcaaaa
    16441 tatgtctata cttcagggta tcgtcgatac agtagtaaac caggtattat tttagctggt
    16501 gaatcttctt tggcagtagc ctatttattt atcctattta cgatcgttac ttgcattatt
    16561 gagctggtat ttaaaaaatt ctatttagtg attgccgcgt caacggactt tgagacaggt
    16621 caacggatga aaagtgcgat agaagatatt tctcaatcct tcttgttatt ggcgtttacg
    16681 attttagaat tacgaatcta taccttaatg ctttcaggta tcggtgattt gcatgcggca
    16741 ggaaagatca atggattctt gtatgtcgtt gctttgattg tactaaccat tgcgctattc
    16801 aaaggctctc aatcagttac gaaaattttc ggcgttgata caagcctgaa aaacggcagt
    16861 aattctcttc ttagcatgtt tgccttagga aatattgcga aaaatgtggg tagcggagct
    16921 aaaaaccttg caagtgctgg taagagtggt ttaagccatt taaatgatat tcgtaaaaat
    16981 ggatttggcc atcgaaaaga tgttacccct gataacgggg aagaacaatc agacgataaa
    17041 gctacaactg tacctaatag taagaaaaca gtttccgaaa tgttcgggaa agcgaaaaat
    17101 ggtgtttcta aagccgcaga aggtcttggt tacgtcaatg aacgtgggtt aaaaggaaca
    17161 gctgaagatc ttacagaaaa agccaaagac accttagaag ataaattaga cggcacgaga
    17221 gctaaagaat tggctgaagc tgtgaagaat ccatccgaag cactagaaaa acaaaaagaa
    17281 aaagcttcag aagtcaaaga aggactaaaa gatgcgtatg gtgaaggaca agtcaatgca
    17341 gcagtgaaag ctaatacaac aaccgaaaaa gaaaatcaag aaggtatagt gattccttca
    17401 gtcaaagatc accaagaagc gttgcactct aatgaaaatt cgattgaaga gaaaaattcc
    17461 atagaagaaa gaaattcaga agaaggaaaa aatggacagt taccaaaaac tgatccagaa
    17521 caatatcgct ataaacctaa cagtgacctt gaaaatgaga agcgaatttc agatttatca
    17581 aaagtaaatc aagaagacaa agcagaaaat gagggacagt taccaaaaac tgatccagaa
    17641 caatatcggt atagatctgt taactccgaa aatgaaaaac gcatttcaga tttaccaaaa
    17701 cgaaatcaag aagtacaaac ggatgaggat tccatcgttc tgccaaatga taatggaatc
    17761 gaagttggaa ctgaaaaatc aacgaaaatt ggtttaccta aggcacaaaa tttaccaatg
    17821 aaagatagtc gtttgaatca attcaaggat aatgatcaaa gttttgttca tcaatcacct
    17881 gcaggtactg ttcaaatgaa accaagtcgt ttgcctgaaa atggaaatct gacaactgtt
    17941 tcttacaatt tatctgaaag tggtgagaca gggaatgcgt ccgttcaaaa tgttgtgaca
    18001 agagaagctg caccaaaacc tattcaaaac gttcatacag gttacaagcc agcaaatgtg
    18061 gcatttactg caccaacaaa aataaatgtg acaagagaaa caaaaaacag gaacaatgca
    18121 ttgaaagaac aactattgag ggatatcaaa caggatatca aaccataaaa ggcctacaag
    18181 ttaggccttt tttctatgta tgaaaaggag aaatcattta tggataaata tagtaacatg
    18241 aaaaaccctc caagatttag tggagaatgg aagtttttcg cttttttctt tttagatcta
    18301 ttaacgcttg gtggctgctt tttttgggcg aaaatggtca ataatcttgt ccacttacca
    18361 ctcttttttg cactcttgaa ttatggctta tggattgcga tagcgttagt gattgtttgg
    18421 cgaccctata ataacccagg tcaacgacaa atttttgcga ttatcgagtc tatttttttt
    18481 gcggacaaga accattatca tgcgttagat cctaaccgaa attatcatgg tacgtataag
    18541 gaggatgaat aaatgcgagt caaaggacaa aattcagttg aaaggaaatc cattttgcag
    18601 tttggaaaac cagaagagcg ctctatcttg gacttatttc catataaatc ctgtacagaa
    18661 gagggagtct taattactag agaagatcat tttcaacgtt attatcgtgt agtttctaca
    18721 gacgtggaag gattaaatga acaagagaaa ctagaacgaa tgaatcagtt gaccaccgtc
    18781 atgcgtacgt atgtgccaac aattaagtta gttagtttga ccacagagac ggatctaacg
    18841 gaacaaatcg tgcaaaagcg gcaattatta gataaaaatc gtatggaaca gtccttagga
    18901 agaaatctac aacgattaaa aaaatatgaa aagaaattag tcgaagaaat ccaggaattg
    18961 aaacgagcag aacaagaacg tcctgactta agcttctttt tcgtcattga aggaaagaca
    19021 atcaaggatt tacaaaccag aaaccgtcag ttaatgcgat ctagtggcat tttagggtta
    19081 aaaccactct acaaaaaaga attgatcaag attctgtatc gaatgaacaa tatgaatgat
    19141 gagtaggaat ggagaagaga gaatgaaata tccgttgata tggttagaaa aagatgttcc
    19201 gaaggaagag accactagaa aatttcctca cactttaagc ctgggtaagt tgggaaaagg
    19261 gcaatctttt aaccaagtaa agaatcaaac aagaagaggt gactgtacat gttagaagca
    19321 attaaaacag ctttttcacc acaaaatgat caaaaagtac ttgatccatt aaagcaacta
    19381 ggaaatcggg aaatagaaat tgtaaaatcc aaagggtatg atttagattt tttaaaacaa
    19441 gtgatgccac aaggtgggtt aaattttgat gaagaatatg tgacgtatgg taatggttat
    19501 tcacgatgtg tgacagttta tcactacaca aaaaatccta cgcttttttg gttggccaat
    19561 ctcatgaaca atcctcatac agtggccaca ctcgatgtta gcacagatga aaagcaaaaa
    19621 gtgattgatc aaatcaatcg tgccttagat gaattaaatg atcgatcaga taatgaacga
    19681 aaagcgactg accgaaataa aagcgcggaa gaatacctgg aactagcgga atatgctaga
    19741 aaattgactt ctaatgggga aatttctaag caagtcaaag tgagagtaca tgtttacgga
    19801 aacacgattc aagaagtaga tgaacgagcg caggaactga tcgaaaatct caaagggatg
    19861 gattatcgtg cggtgactta tctgcataaa accaaacgag attggcaatc gatgtttacc
    19921 atgttcgatg aacaagctga atggttcgga aatatcaaag ggcaatcact accatcgtta
    19981 aacattggcg gaggtttccc gttccaccac caaacgctaa aagatccagg agggacacaa
    20041 tatggggaaa ccatgacagg cggcccattt gtctggaatg cgacgtataa aaatagtgta
    20101 cggttgtcta ctaatacctt gcttctagga atgatgggag ctggtaaatc gaccgttctt
    20161 aaaatgatcg cggaagccca tttagctgca ggagacttta tttggggctt tgaaaaggga
    20221 aaagacttta ttcctttctt aaaggaatat aacggtatta tggtgcgttt agacggatct
    20281 gacggaatga tcaatccttt agagatattt gccacacgga cgtatgatga agcatccagt
    20341 ttatatgacg atggatcagt caaagatttg aagatcaatg aagcagcgtc ctatcaaaca
    20401 cacttagaca aagtggtcta tcaggtacag ttagtgagtc ctcagctaaa aggaacgatg
    20461 aaagcagaat ttaagaccta cttaaatcgt ttttatgaag agtatggaac agtaccacgc
    20521 ggctttactt cctcaaattc gcgttcaaat actgatacgc aagtcacagg gaaagatcca
    20581 gaagcttatc cgacgttcaa agaattttta gactttttag gacaactgga attaccaggt
    20641 gcttcccaag agaaaaagaa tcgaaaagaa gaaatggaat ccatcgtaga atccttatgt
    20701 gagacgtatg gcatgatttt tgatggtcat agtacgattc gacaccttga ccaacaacag
    20761 ttggtctttt ttgacattga ctctattaca ggcttagatc gaggggtaca acaatgtcaa
    20821 atgtacatgg ctatgacgtt gatttggaat caggcattga ttcaaggaag aagacaacga
    20881 cgtttaatta aagaaaaaca aatggaagaa caagacttca aacgcttctt ggcgatgatt
    20941 gatgagtgcc ataatggtgc gacctgttct gcagtgaaaa gctgtgggtt gcaagtagtc
    21001 taaaaactat tgtaataact cttataacga gaagtcaaat gagtgggtaa cgccacaaag
    21061 ggcttccact gatactccga catgcaacaa gtagtttatg cagaccaacg ttgtgtgaag
    21121 ctcggtgaag tcggctgaag gataccgtaa ggtaaaagaa atagtgaata ccacgaaagt
    21181 tctctagagg taaagggtgt atccgataag tcgggtgtcc aaaaaaactt aatatggtga
    21241 gtatgtacaa acgtactgac gaactagcga atgtactgtc cgaaaggcat agctcctaga
    21301 aatgggagag acagtgagca aaaaatcact cattgtgcta acttgtggtt gagtaaaagt
    21361 cgttgttatg aaagaccatg ctgcgttaca ggcgtaggag aagttagggg gcaaaagcta
    21421 gcatctaagt taaggcatgt ggatagatat aagggaacag gggaagccat caacatggag
    21481 agtgcatact cgatgaagtg gtagtaagaa atatgtgaat gacaaataac ttactttagt
    21541 gatggtgaaa gtagagtcac gactgatgaa ggttctgtaa tggaactgga ggaacaggct
    21601 cgattcgtct gtaaaagtca tgaaacccgt tcaaccatgg gattctcgta tgactaaaaa
    21661 ggtcacgagc caattggagc gtgataaacc atgacaaaag aaacaagtcc aaaagatagg
    21721 aagttgcggc atgcagaata ttatggcatg acggaaatct tcgataagat atactccaaa
    21781 agtttgaagg gagataattt caagaatcta atgcagatta tcatttctga taacaacatt
    21841 gtcctagcat atcgtaatat caagagaaat cacggtagtg tgacaagagg agaagatgga
    21901 gtaacgatta aagatattga aaatctcaac cgtaatgaat tcgtagaaat tgttaggaag
    21961 cgattcgcac attataaccc tcggaaagta aggcgtgtag aaattcctaa atataatggt
    22021 aaattaagac cgttgggtat tccatcaata tgggatagaa tcgcgcagca atgcatctta
    22081 caagtattag aaccaatatg tgaagcaaaa ttcaataatc acagctatgg tttccgccca
    22141 aatagatcag cagaacacgc aatagcagat tgtatggccc gaatgaatca aagtcacatg
    22201 gaatatgtgg tagatgtaga tatcaaaggt ttcttcgacg aagtcaatca tactaaactc
    22261 atgagacaaa tctggtctat gggtatacga gacaaacagc ttctcttaat cattaggaaa
    22321 atgttaagag cacccgtagt acttccaaat gggaagttga tatacccaac aaaaggaaca
    22381 ccacaaggcg gaatacttag cccattactt gcgaacatca atctcaacga atttgactgg
    22441 tggatagcaa atcagtggga ggaaaaagat gccaaagagc taaaacatca ttttaggaaa
    22501 aatggagctt gggataaagg taatatctat cacaagctca gaaagagtac aactctaaaa
    22561 gaaatatacc tagtgagata cgcagatgat tttaaaattt tctgtagaaa ttataatgaa
    22621 gcgaaaaaag ttttctatgc aactaaaatg tggttacagg agcgtctaaa gttaccaata
    22681 tctgaagaaa agtcaaaaat cacaaatctt tcaaaagaag aaagtgaatt tttaggcttt
    22741 acgatgaaat tagtgaccaa aggtaacaaa aaggttgtta atacgcatat aggtacaaaa
    22801 gcacaaagca tgattaaagt gaaactaaaa aaacaaatca aacgaattca aaaaagtcct
    22861 aatagcaaag atacctttat ctatattgga gtctataaca gtatggttat tgggattcat
    22921 aattacttca aaattgcgac tcattgtagt gtagcaatgc gaaaaattgc aagagaactc
    22981 agaccaagtt ttgaaatacg actacgagaa caaggaatct caaaagaagg acaatacgga
    23041 aagtacgaag gatacaagcc ttacctacgg tcaggacaaa tacgatatat aagagggttt
    23101 ccaatattac cagtagggta tattcaacac agacatccga tgtgtaaaaa aagaaatatt
    23161 aataaataca ccgaagaagg tagggaagcc attcataaga agcaacaaac cgtagaagaa
    23221 tggaagatat cgtggttgtg tagcaatcca gtgcttagta agagagcaac gattgaattt
    23281 gcggacaatc gaatttcaaa attcatcgct caaaagggaa gatgtgcagt aacaggtgag
    23341 gagcttatct tatctgaaat gcattgtcat catattatcc cttatcatga aagcaaaagt
    23401 gatagctatg aaaacctagt gattgttact gaggaggttc atcgggtaat acacgctaca
    23461 caaagtgaaa cgattgagga acttctaaaa tatctaaaat taaatccaaa acaaaaagaa
    23521 aaactcaatg agttgagatt aaaagttggt aacgaagaaa tatcttaata aatatttgta
    23581 ggacgggaaa acagaatgtc ttttatagac gagggaacgc cgtatgcggt gaaagtcgca
    23641 cgtacggtgt ggactggggg aaaagacgga gataatatca aactcttacc tatcagtatc
    23701 gattaatcca gattttattg aaacggtaaa ttatgtcaca aactttcaac gagaaatgcg
    23761 taaagtagaa gcaatgactg ttttagctac acaatcccca caagaaatgg tgccagaaaa
    23821 tatgtcttct gataacttct cgaaactgaa gaaagtcttt gaattttctt ctacgaagat
    23881 ctttatgcgt atggatgaat ctattttacg tcatatagaa ggattagtgg gtaaatcaat
    23941 gacgaactca gaattagaat ccattcctca acaaaatcaa ggagacgctg tgatcaattt
    24001 tggctcaaaa gaaaccattc gtgttcattt tagtccgaac aaacgtcaat taaaattgtt
    24061 tgatggagga aaataatgga aaaccaaaat gaaagtttaa tcaagcagta tgttaagcgt
    24121 cgtgcaaagc ggcgcttatt tttgtggttg tttggtactt ctgcaggttt gattactatt
    24181 ttgatcactg tgttcgtgac cctatttcta atacttgcgg cgggctcgat tgataattcg
    24241 gattcagatt caagcagtgg tggcgaagct tttacaggag aatattcgga aggcttaccg
    24301 atttataaag aaatcaaagg aagaggccct ttttctgatg agattgcgca atatgccgta
    24361 ggtgcagcag tgaaatataa attattacca agtgtgatcc tttcgcaata cggttacgaa
    24421 tcagcgtttg gtacatcggc gtccgcaaga aatgatctca actattttgg gattacttgg
    24481 tttgatggtt gtctgtttcc taaaggaact gcacgaggaa ttggtggcat tgaaggtggc
    24541 tggtacatga agtttcccaa ctcaaaagct gcattttcct attacggctt tatggtagcc
    24601 acacaatcca attttaatgc ttgtgtagga aataaaagcc ctggcgcaag cttattgatc
    24661 ttaggacgag gcggttatgc cgctgcagga attacggaag atagtccgta ttatacaggt
    24721 tgtatgtcaa ttatcacttc taataaatta accgaatatg atgaattcgc catcaaacat
    24781 tggggtgaag gtggcaataa caacggcacg attactggtg aatggacgaa tccattccct
    24841 ggcagcagtt tagataaaag ttccttcagc ggtggtcaat tatttggaac gaatccaggc
    24901 ggtgaattcc gtccgaacgg cttccatgat ggattagatt ttggttcagt tgatcatcca
    24961 ggaagtgaga ttcatgcggt tcatggcgga aaagtcgtgt acgttggcaa tcctggtatt
    25021 tcaggtttgg gtgcgtgtgt gatcgtgatt aattacgatg gcttgaatat ggtgtatcaa
    25081 gaatttgcaa atagtacagg aaattctcga gtgaaagtag gcgatcaagt gaaagttggt
    25141 caagtaattg ggatacgaga tacagcacac ttgcatttag gatttactcg aatggattgg
    25201 cgtcaagcac aaggacatgc attcattgat gatggtacat ggatcgatcc gttaccattt
    25261 ttgaatagca gtaaaaaata gatcaggaga aaaaatatgg aaaataaaac gttaaaaatc
    25321 gtcttaacag ctgcaattag cgttagttta atcggcaata ttgcgttgtt ttggcagctg
    25381 aaagaggaac aagcagcaaa tacctataaa attgaagaag tggctaaaaa cgcgtctgag
    25441 aagctcaaaa aaaattcctc tgaggcacag gcgaagatcg acaagttaaa tcaaaccatc
    25501 aatgagaaaa accaagaaat cgatcagctg aagcaacaag atggcacaaa agcaaaagag
    25561 gaagtaactg aagcacaaag aaagacggca gaagagttcg gtcaattggc cattgataaa
    25621 tcattaagca gaaatgaatt aacggaaaaa ctaaaagatg tcgcaaccca agaagtgatc
    25681 gacaaattaa gtcctacaga cgatgatcac caacatgatg attacggaag ttactccttt
    25741 acattaggtg acgtgcaaac ttacgctcaa acaagcagtg taaaagagga acaaaatttt
    25801 gtcgtatttg ttgattatac aatcggcaat ccgcaattca aagatgtcaa accacaaaaa
    25861 ataaaaggtg gattaactgt cactgaaaaa caagtagatg gtcaatggaa agtcatagac
    25921 tttagttatt tcactcgtta ggaggaagaa aatgagtaag aaaaaaggag aaagacaacg
    25981 tttcaaaaaa cgaaaaagtt atcttagatg gctgggaatc tttatgattc tcggcttttt
    26041 gattcttgct agtccgattt atacgaatca gaaggtagat ataccagatg aggtaagcac
    26101 gaacgccttt ttaaacacga aagagctgac aatggtttca aaagaaaaag gagcgaacca
    26161 gcaattagtt tttgaatttg cgatcgatga tgaagatcaa agcttgttaa aagaactggc
    26221 taatcttaat tatcgcgtag aagtaaagac agctaaaggg gattatcaag caatacgagc
    26281 caaagtgatt aaggtgtctg atgattattt tgcagtgaag ctaacgcatg taccagaaga
    26341 atatatcgcc atgcgtttaa ccattattcc tgaaaaaatt gatccaaaag tggatatgca
    26401 agaaccacaa gatctgattt tttatattca cgaagataag gtcaaagatc acgtgaaaga
    26461 cgaagattat gaacaacatg cgatcaatta caaagtgaaa ggatacaaga aagaacaaaa
    26521 agcagctcaa aaacaaatgg aatctttcca agcaaccatt gatttgaatg aagaattagt
    26581 gaagaagcta aaagatcaat tgccttatca agtcgcagat gatcaggaaa atactgaaaa
    26641 caagattaat agctaccagc aagaaattga aacatcgaaa acgcaactga aagatcaaga
    26701 agagatcatt caaaaacttc aagaaaaaat tgatcgtatg acaaaagaaa agatttagaa
    26761 ggacaggtgg agtgaaaaaa tgaagcgaac aaacttggct gacaaagata ctttcgctga
    26821 gcagaaagta ttgattaaag cctatctcaa agttgtaaaa gctaaagaaa tatgtcgaga
    26881 agtgaattta tctaacacaa caatatctga gttgaagaat ggtcgaaaga atttagatac
    26941 cttaaaattc agatcatttg aagctctata tgtagctgct aaaaaatatt ttacagctga
    27001 taacaaataa aaaacaggag tattttatgt gaaaaaagta tataaaaaat gcttatgaat
    27061 cagctatatc accttaacca tttgttaatg agatgggaga aaacattaac cacgattcta
    27121 acgcaaacat caagcacata aaacatttaa cactcctcct ttctggctga agatttcaac
    27181 caaacaggaa gacagcgtat caattatagc atacactttt tcttaaattc atttttcata
    27241 tgacaaaata tttccttcta atcgacaaag ttagaaggat tttttattgt ctaaatacac
    27301 aaataacctt aatttttaaa gagcataagg aaacgtgata tcactttctc acgaaaggtg
    27361 ttaggtaggc tgtgcctacc acgtttctaa gaaagataaa tgatatcaaa attttggatt
    27421 tgtgtgtaga tttgtgggta ggatgaaagc gaatgtgggc agtaaaataa gcgtttgtgt
    27481 gtagcaaaat caaaaaatgt gtagggatat gtgtagctac acatatccct acacataata
    27541 aatttggagg aaaatatgga cttattaatt agaaatattg aagaaaagta tattaataaa
    27601 attgacaaac gttgtgaaga acttacggtc aagactggca aaaaatggag tcgaaatcaa
    27661 tacctaaaag ttttgatcga aaatgatttt gatcacgctc ttctgaatta taaaaaagac
    27721 caatttgatc ggttgttaga aaaatttgtg gatattcaaa cttataatac gaaaacttta
    27781 gaagaatata ttgcaacaaa taatcaatta attggtttgt taattgaata ataaaaatag
    27841 tattgtgaat ttataaaagg aggaaatcaa ttttggcaaa agcaacaaga aaacatgtat
    27901 atctttacga tatgcatatc gaagtacttg atgaaataat ttctactcat gaagatatat
    27961 ctaattatag tgaagcagtc agacatttgt gtatgatgta tgatccaaaa atcgaagaaa
    28021 aaataaatcc aattcaaatt aaattgaatg caatgggaaa agaaatttct attttaaccg
    28081 aaatggtttc agagtttgcg gatcttcatt tgaaagatac aggaatacta attggtacaa
    28141 attcaactgt ttatcaagaa gcagaagaac gtgtggaagg aaaaattaaa tccaatcaaa
    28201 ccaaaaaata ttcttctaag ttaaagaaac cagtgaatga atctgttcat accagagtag
    28261 atccatttga tccaacgaag attctaaaaa gataaaagta gaaggagaac gtgatgaata
    28321 aatcgcctgc agttattctt acttgtcaat ttaccttgcc caataaaaaa tctttttcaa
    28381 catacattga ttatatgaca agagaaaaag cactggaaga aatggaacga cgtacgcccg
    28441 aagaagaaca ggaactgcaa cgaatcaaga atgctttaga ggatttttat attcctgaag
    28501 gagaaacttt ttcggaaagt aagacggata aagaattcag tgaaacgtcg aatgaagctc
    28561 aatcgttgat ggaacatgga atcaattttg acaagttaga ggaacaagat tttaccaaat
    28621 atctttctta tatgacccgt cactatgcgt tagaacaaaa gaaagaactg acgcaaaggg
    28681 aacaaaaaga ataccaacgg ttgaaccaag caatgaaaaa atatcaaaca gagccacata
    28741 aaaaagcttc aggaaatctt cctggagttt tttctatggc ttcagacgaa gtgaaaggaa
    28801 gtgatctgaa ggagatcaaa caaatcttcc aaagaggaca acaaaaaggc tcaatcattt
    28861 atcaagatgt tatctctcac gacaattcgt atcttgaaaa aatgggcttg tacgatccca
    28921 aaacagatac cttagatgaa gaaggtttga aagcagctgg aagaaaaatg atggagacat
    28981 tattcgagaa agaacaattg aatgaaacag gttactggat ggcaacgatt caccgtaaca
    29041 ctaagcatat ccatattcat tttgcagttg ttgaaaaaga aaatacgcgt aaaccgtata
    29101 ctgagattga aaatggtgtg agttatatcg taccgaaggg gaagagaaga caagcaacat
    29161 tagacgcaat gaaaaccaat tatgtacatg agttagaacg tttctcttat gaaaaggatc
    29221 gaatgatcgg tatggagaaa cgaaatttat tgacacgaaa aagtgatttg cgtaacacct
    29281 taacaaaagt agtaaaagaa ccagcacgtt acgatcgaca cgccatgaac ttattaaatg
    29341 aagtatataa aaccctacct gaaaaacgtt cggaatggaa ttatggagat gaaaatcgaa
    29401 ccaagctttc accatttaca aggggaaaac ttgatgagtt aaccaaatat gttttaacaa
    29461 aagaacctgg atatcaagaa tatatacaga tttcaaaagc attgaaagaa gaagccaaaa
    29521 gcctttatgg ggaaagcaaa agagaaacaa agaatgcaga aaaaaacgcc ctctttgatt
    29581 taaaaaagcg cacaggaaat gcgattttaa cagaactgaa gagacaggat agtcaaataa
    29641 aaccattaat agaaagtgtc aatcagatgc aatctattta caatgaacga tcctttcgaa
    29701 cactcaaaca gttgccaaca aatgaataca agtatagtta tcaagaattc gtaaagcgta
    29761 ggaaacaatt ccaacaaaga gttgtcacgc agcgaagctt gcgtcaatta aatcgaacaa
    29821 ttaatgcaca tgaagaaaaa taccacgcaa gtaaagcata tgaagagcaa cagcgaagaa
    29881 tccaaatgga acaagaaaga caataggagg aagagaaatg aaacaatatt tagatcaatg
    29941 gaaagtcata gaaggatcac taaaagaaga acgaatcgaa caacttcctg attgtttaga
    30001 aaaagagcat ttgtttcaga taagcgaaat gttgagaaac gaacagtttg atccaaatca
    30061 atttttagtt gttgagtacc cagctactgg tgtgtattgt tgtaatcatg tgaagggtga
    30121 aaaatatttt attattcaag aatacgaagg taagttagcg ccttattata ctacttggga
    30181 aataaatgaa gagggaatca ataatttccc atgtgaaagc attgaagagt ctatttctct
    30241 cacggaatgt taaataaata gagacgtaaa agagtcctga acgtgattgt ccaggactct
    30301 ttttcaatga attgttttcc gccaattcac tgagtggtgc gattcaattt tattatatca
    30361 aaagaaagtg agattccaaa gtgaagggag gaagaagaaa tgactcagac aacgattggt
    30421 gttgtggttg aaaaaagcag ggataatctc gtttttgtga cagaaattca aacaggtaga
    30481 gcttttgtag tcacagacaa agctgcaaaa gcgtatcaaa atggcaatat tttaacgtta
    30541 aatctgacta cgaagatgtt cgttgatgca gtagaaaatt atccatttgt ttagacaaac
    30601 gatattttac aaggaggaaa cggacatggc agaagaaaaa gaaccactag ataatagtcg
    30661 gttgggaaag tccaaaagaa aattagtacg gttgcaaaac gaattaaata atcaatttca
    30721 aaagctattt gatcaccaaa aattgacaaa tgggcaacca atgaatgaca aacgaaatgg
    30781 tcgttcatgg tttaaccagc aagaaaaaat agaaaataaa atacaaagtt tgagagaaga
    30841 gatcaagcaa caagaaaaac aggtagaaaa acttgaaaga caagaagagc taaaggaaat
    30901 gggctataac aagtacggcg gactggatat gacgatcgat aatattccaa gaatcaaaga
    30961 agaaatcgaa cgctttgaaa aaggtgaatc gactttttca gccgccacga tccgaaaata
    31021 tcaacggaag ttggaaacat tagaacagct gaaagaaaaa tcagaaaaag gaaaagaaaa
    31081 tatcctccct gaagtacaag cgatcattga cagcggacga gtgacgcaat ggaaaaaaaa
    31141 tccaaccatt tattttttga aaggctatcg aaaagttgct ttagaactga atgaaaacgg
    31201 gggatttgag gaatcaaaaa aataccaagc cacgtcggaa gaggaaaaag cagctgttcg
    31261 tgaattactt gaagaggctg caccaactga ttctctgaag gagaaagaaa aagatcattt
    31321 ctatcgagta gaattcaacg aaaatgatcc agaaaaagga ttgaaaagtt atggtgggga
    31381 gatcgtaaca ttagaattga tcgatcaact taaaacgttg gataaccaat acgctgcaga
    31441 agcaggttat tataaattct attttgaaga agtgaagaac ggagaagtag tagatcagat
    31501 ccgaatggat ctaggggatg gcgtcaacag taatcacccc atctatttag agttagcaaa
    31561 attgtgcgaa caagtacctg agaaggaaca aaaacaatcg gtcaaaaatg aacctgtttc
    31621 gaaagaacaa atgcagtcga aagaaacaaa aacaaaacaa aagggctatc aaaaacgaac
    31681 ggcagaagag atcaaacaag aagttaaatc cctttcgcaa caggcactgg atgcggtgaa
    31741 aaagcatacc cattcttctg aagacgtcaa agaacttttg aactttatgt ctcgttttcc
    31801 agaacgatcc tttagaaatc aattattgat tgaggaacaa tttccaggtg caacggcttg
    31861 tttaggtcgt gccgcgttga aaaaagaagg aatctatatc aaaaaagggg aacaagggaa
    31921 caagatcttt gtcagaaaaa cggtcaaagg attttatgag aaaggacgtg gctttgttcg
    31981 agaaacagaa gctacaccag aagataaaaa acgaatcgaa agtggtcaaa tcgaagtgat
    32041 caaaaaacct tactacacga ttgaaaaagt gtttgatatc acacaaacac agttaaaacc
    32101 tgaagactat ccgaaaatct ttccgaaccg tgtctttgat tttcaattgg ataaaaaagg
    32161 acagtcagaa cttcaagctg ggattcaagc ggtagccaac aatattggaa tacaaattcg
    32221 agatatgaca gaaagtgaag tctatcaaag agaattagga caagcacgtg gggcgtatgt
    32281 tcgtaacgaa tttactggcg aagaagaaat cgtcatgaat actcgaaata ctcctacgca
    32341 gcatttagct acaagtattc atgaattagc ccatgcaaga atgcacaaat tttcagaatt
    32401 agataccgca actaaagaat tgcaggcaga aatgacttct tatattgtgt gcaaacattt
    32461 tggaatggac acttcggaga aagctattcc ttatattgca gcatggacga agaatggtca
    32521 gacgttggat gataaggaag cggcggaacg tgggaaaatc atgaatgatg tcagccgtgt
    32581 cgcaaatgaa ttcattcaaa ccatttctaa tgaaatcaat caatacagag aaagagaacc
    32641 agagatccaa ccagcagaag tgaagcagga gcataaaaat actgtagaaa cggcagagaa
    32701 taagaatatt gtgcccgttg gttcactttg gatcgacaag aaagacggga aagtaatgga
    32761 agaagatacg ggtcgaacgt tacatttgaa ggatgcggcg tttattgaag atccgcagca
    32821 taatcaagtg attttaaaag gaaaaagtta tggaaaagag attcaaacgg cgttaccttc
    32881 agaagaattt gcgctaggaa atccattccg attttcactt gaaacagtgt cagaagtggc
    32941 caaacgaaaa aatgaaatct attatgaaca acaattgtca caaaaagaac gcgtataaaa
    33001 caagtaaaaa agactatctg aaaaacggat agtctttttt cttttgggag gtttgaaaat
    33061 gaccgaaaaa aaaatgagtc ttattgatcg atgtaaacag atcgatatag tggattttgc
    33121 tcgaaacaat ggaatggctg tagtcaataa aggacgagac tatcgcttag aagaccatga
    33181 ttcatttgtt tttgatcgaa gaaaacaacg tttttattgg aacagtcaaa atattagtgg
    33241 cgacattatt gaattagcta aacttttttt catagacaaa gagatccaag actctaagca
    33301 acaatttaaa gcagccactg attttattct aaagaatgaa gacaaaacag aacgagtgga
    33361 aaatcttcat tttgaaacag aaaagtacaa agatcacccc gttgattatc aaccactcac
    33421 agaaaaagga aggaattatc tgaaggaaga acgaaaacta ccagagtggc tgattgatta
    33481 tgcagaaaaa gaagggttga ttgccgaact aaagccaaaa tatgaacggc aaaatttctt
    33541 agttcgagac gatcgtttag atcatgcggt cgccttttta tggaaagatc cacagacaaa
    33601 agagacagta ggggcgagtt atcaaggaac gttcattgat tatgaacgtt ttggtgagag
    33661 aggcacatat aagcatatag ataaaaattc gacagcgaat catggcttta acctaaagat
    33721 cggtgaccct aagcagttaa aattttttga gagtagtatt gatttactta gctatgctgc
    33781 actgaatcgt gatcagttaa acgatacgtg gttagtctct atggaagggt taaaacacca
    33841 tgtgatcagt cattattttg gtgaggcagt atctgaattg agaaagaaac aagcctttcc
    33901 tcaatccatc gagatttgtg tggataatga ccgtgcagga catattttct acgaaaaaga
    33961 acaattaatg ggcgcagtcg atccatttac gaatcagaaa gtacgttgtg aacgaggaat
    34021 cgctaatgat tggcaagtgc caaaagaata caaaatcatt tatgaagaag tcgcaaaaga
    34081 agaaaaggtc acaccagaag ctattatggc catacacaag acagaaaaca atctgcaatt
    34141 aacgaaccaa ctggtttccg ctcacaaagt aaatgcgttt tttggccagc agctttctgt
    34201 taacgattca atagaagcca ttaatttgaa agatatttgt agagaagtcg caaaagaatt
    34261 aaaagtttgt gaacgcgtgg atggtacgta cgattttgat cgtttttatc aagaaaaagg
    34321 ggatattaat gcgcagattc ttttctctta taaagcagaa caatactaca aaggctataa
    34381 aaatcatgaa catgaattta tacctgaagt aaaaaaggac tggaacgatc aattgaagca
    34441 tgagatccag caacaagaaa tcagaaagca aaaacgtgcc atgttgtttc aacaaggcag
    34501 acaacaagaa agggagtgaa atcgtcatga tgaaaaccgt tattttagca gaaaagccgt
    34561 cacaagcgaa agcttatgcc gatagctttt ctaaagctac ccgaaaagat gggtattttg
    34621 agatccaaga tcgattgttt acaggagaaa ctgtgatcac gtatggtttt ggtcatttag
    34681 tcgagttaga ttcgcccgat atgtacgatg aaaaatggaa acaatgggca ttagaacatt
    34741 tgcctatttt ccccaatcaa taccactatc atgttcctaa agataagaaa aagcagttta
    34801 gtgtcgtcaa acgacagctg cagtcagctg atacgattgt gatcgcgact gatagcgatc
    34861 gtgaagggga gctgattgcc tggtcaatca ttcaacaagc aggcgccaat caaggaaaaa
    34921 cctttaaaag actttggatc aactccttag aaaaagaagc catttatcag ggattccaac
    34981 agttacgaga cgcagaagag acctatccaa aatttgaaga agtacaagca cgtcagatcg
    35041 ccgattggtt aattgggatg aacggtagcc cactgtatag tttactcttg cagcaaaaag
    35101 gcataccagg aagtttttct ttaggtagag tccaaacccc aacactctat atgatttatc
    35161 aattgcagga aaagattaga aacttccaaa aagaaccata ttttgaagga aaagcccaag
    35221 taatcgctca aaatggcgca tttgatgcga agcttgatcc aaatgaaacc caagcgacac
    35281 aagaagcatt tgaagcctat ttaaaagaaa aaggtgttca attaggaaaa cagccaggta
    35341 cgatccttca agtcgaaaca gagaaaaaaa gtgcggctag cccacgtctt ttttctttat
    35401 cgagccttca gtctaaaatg aatcaactca tgaaagctag tgccaaagat actttggaag
    35461 ccatgcaagg gttgtatgaa ggaaaatatc taagctatcc gcgaacggat accccttata
    35521 ttacggaagg ggaatatgcg tatctgttgg atcacttaga cgaatacaag cattttttaa
    35581 aagccgaagc gattccgact cctattcata caccgaatag tcggtatgtg aataataaga
    35641 aagtccagga acattacgcg atcattccta caaaaacggt tatgacagca gcggcttttg
    35701 aacaactaag tccactgcag caggctattt atgaacaagt gttaaaaaca acggtggcca
    35761 tgtttgcgga aaagtatacc tatgaagaaa caacgatctt gacgcaagtt cagcagcttc
    35821 agttaaaggc aatcgaaaag gtgccgctag atctaggttg gaagaagtta tttggaaaag
    35881 agagcgacgg aaaagagaag gaagaagagc cgctacttcc taaagtgaca aaaggagaaa
    35941 tggtgacggt ggatctacaa gttcttgaaa aagaaaccaa gccaccacag ccttataccg
    36001 aaggtacgtt gatcacggcg atgaaaacag ctgggaaaac cgttgatagt gaagaagcgc
    36061 aatccatatt gaaagaagtg gaaggaatcg gtacagaagc gacacgggcg aatattattg
    36121 aaaccttgaa acaaaaggaa tatatcaagg tagagaaaaa taagctcgtg gtgacgaata
    36181 aagggatttt gctttgccaa gccgtggaaa aagagccgct gcttacgagt gccgaaatga
    36241 ccgccaaatg ggaaagctac ttgttaaaga tcggtgaacg aaaaggaaca caaaccactt
    36301 ttctagccaa tatccaaaaa tttgtctctc acttattgga agtcgtacca gggcaaatcc
    36361 aatccactga ttttggatcg acgttacaag aagtgaaagc agccagtgaa aagcaagaag
    36421 ccgcgcgtca tttaggaatt tgtccaaaat gccaggaaca agaagtctta ctctatcaaa
    36481 aggtggcagc atgtacgtct gaagcctgtg atttcaaact atggacaacg attgccaaaa
    36541 agaaacttac agcgacacag ctgaaagaga tcattcaaaa tagccgaaca agccaaccag
    36601 tgaaagggtt aaaaggtcag aagggttcgt ttgaagcaac gattgtttta aaagaagact
    36661 tcactacagg ctttgaattt tctgaaaaga aaaaaacgaa cttcaagaaa agaacacgac
    36721 gcactacaaa ataatggagg gttaatctta tgcgtaatac aacacacaaa caacaagaaa
    36781 tcgaaaatca aaaagccaaa attcgttcag aacaggaaaa aatgcgtgcc agcaaacgaa
    36841 gactgcagca attgaagaac cagcagcgga ttcaaaaaga aatcgaatta gatcgataaa
    36901 ttcctttctt gttaatataa aactaacatg ttatagttgt gttagtttta tattaacttt
    36961 aggggaagag taatgaaaga caaaatccaa tgtatttttg ataaacataa tggcaaccta
    37021 tccttgaagg atgctagaag tgaaggatta tcgcctatga cgttagatcg attagccgaa
    37081 aaggataaaa tagaaaaggt agcaccaggt ttctatacct taaaggatgc gttcgtggat
    37141 gaattgtatc tcgctcagag tatctattcc agagggattt tttctcatga gactgcgctc
    37201 gatctttttc aagtaggaac atatatccct aaaaagatca atatgatgtt tcctaaagga
    37261 tacaatgtat caaaggagaa attagatcag tatgccattc aaccgcatta cactaataaa
    37321 gaaaattttg acctgggagt cacagaaaca gaaagctttt acggcaataa aattttagtc
    37381 tatgatttag agcgaacgtt atgtgacatg tggaatccaa gatacaaagc aagcgtagag
    37441 gtaaaacaag aagcattaaa agaatatatg acaagtacca gtagaaatac aaataagcta
    37501 agaagataca tggatatttt aaaatcacca aaagaaatga ctttgtatat gttgccactt
    37561 tattgagagg agtaaagatg aatgacaccg acacaattta aagagaaaac aagaaatttc
    37621 gcagcagaga aacatttaaa cccacaaatt gtccaaagat attatatgat ggaaaaatta
    37681 ttagaatgta ttgttgaatc gccttatcat gatgattttg tattaaaagg gggatttctg
    37741 attggatcaa aatacgggtt agaaaaccgt tcaaccgttg atattgatac gacttatcga
    37801 aattcgaaac taacaaaaga aaagttagaa actgtttttt ctgaattaac agctaatcct
    37861 acaaaagatg gaattcgttt tgaattaaag ggattaacag agacgaggga agccgattat
    37921 tatccaggtt ttcaagcaaa gtttattgct tatcttgaaa atgcacgtgt accttttaaa
    37981 ttagatatta cttcaggtga ttctattatt ccagcagcat tgatgtatga acacaaatta
    38041 atgtttgaag ataaaacgat tcatattcca gcttatccta cggaacaaat tctagctgaa
    38101 aaattacatg ccacgtttag ttttggaaaa gataattcgc gcatgaaaga tttttatgac
    38161 ctgtatatga ttccgaaatt agaacgtatc gatgagaaaa atttgtataa gagtgttcaa
    38221 ggaacattca aggaaagaaa taatcaatcg gtgtttaagc atttgtatga tcaggaaatg
    38281 cctcaattgg tgaaagatga gggcttgaga gaaaagtgga aaagatacca acaagaaaac
    38341 tattttgcca aagacatttc tttcgaatct acactaactt ccgttgattc cctgatgcaa
    38401 tcaattgtag agcaagagaa ggcagaacga aaacaaaatt ttaagagatc acaacaaatg
    38461 aatcaagaaa tggagagata aagatgatca ataatgtaac tttagtaggg cgattaacta
    38521 aagatccaga tttacgttat accgcaagtg gcacagcagt ggcgactttt tccctagccg
    38581 ttaatcgtaa tttcacgaat caaaacggca atcgagaagc tgattttatc aattgcgtga
    38641 tttggcgcaa accagcagaa acgatggcga cattagctaa aaaaggcagc ttgatcggcg
    38701 ttgtcggacg aattcaaaca cggacttatg acaaccagca aggacaacgt gtgtatgtga
    38761 cagaagttgt ggccgataac ttccaactat tagaaagtaa agcagcgaca gaaagccgta
    38821 tgcacgctga tcaatcgtcc agcagtccaa gccctaccac ctttgaacaa cgggatacag
    38881 cgacgcctaa caacaatgga ttgaacgctt cacagaatcc atttggtgga caatcaatcg
    38941 atattagcga tgatgatcta ccgttctaag tagcattttc tattggttgc atttgctacg
    39001 gtttatgtta taatttagac aacaaaaaga attttcaatc tataaccaaa aaccactagc
    39061 tggcgggcta gtggctttct tttacgtttc tattataaga attaacgggt aggcttactg
    39121 tctttcttgg caaatttaca atcaagccat ttgcagatat agtgcgcggc tacttctgcc
    39181 atgattgcaa aaacaaaatc aaaaagaatt ttcaaaccta taacctcctc cccttgccag
    39241 attaggccgg ggtagcagac agcaagacta ttatagcata attgaaatat ccaaatgatt
    39301 ggatttttgc tataatttta gcgaggtgat tgtgatgaac aaatatgttg gtttgcttga
    39361 taaaatccga gtaataaaaa cgcaaccgtt attggttcgc tttactttac aaacgattca
    39421 tgaatcgatt aattgtgtag ttgcagatat tgaaattatc gataaattgc taattatgga
    39481 tgatggaaaa tacaatattg ccgtcacagg tcacttcaat aaacgaaatc agctagttat
    39541 tgctagcatg tatgttagaa atcccgatca ttttacgcga tcgatgggca tataaataaa
    39601 agaagagttg tagtcctaaa tgaggattgc agctcttttt gttttacata gaaacaggaa
    39661 aagacctttt taggaagaca caagtgcctt ctcaattgaa aaacctttct ctcactccaa
    39721 ccgccaccgc cgttcgagaa gaaaggaagt gaacgctgca cgttcactaa gatatggtgt
    39781 cttgcaaagc aagacaaaat agacaaaact tcctctcacc aaaaggttcg ttcctgttcg
    39841 ttatctcttt attgatttgc cagaccggca acaagttgcc ttttatcacc aaaaagcgac
    39901 aaggacaagg gtaaatggca cgctgcgcgc ccttgtcctt gtcgcttttc gttgatttat
    39961 tctggccaac aaatcaataa agaaagaact aacgaaagga agtaaaacct ttcctaagag
    40021 gaggcagaat caaacatgcg aaagaaattt gattattggg gtgttccctt cagcgaatta
    40081 ttcccaaact atcatgcacc acatactgtt gaatgcgatt gtggagaaag agcgaaatgt
    40141 atcaaaagct atcgtttgta tcaatgtcca acatgcggga aaaaatatac cttgtcttat
    40201 ggcgattaca ttttgattga tgagaaagga aagaaacgct gatgaaaaca caacacaaat
    40261 tattgactgt aaaagaagtc gttcaaattt tggtggacaa agaagtcatt tctgaaaaag
    40321 tagcagaagc tttacttatt ccgcggtact ggacgataga aaccccttta gtagaggatg
    40381 caccgactat gctatttgaa gacctagtga agattgcagg atatgaactg cattatcaag
    40441 aaaacatgga aacttatgaa atcgaatatt ggttaaccaa attagagaac taaaaaggag
    40501 aaaaaatatg gctcaaaatt actatgacga atttgtcaaa ttacctttgg ataaaatggc
    40561 acaaaaaatg gaggatatga cgttcctata ccatgaaaca cgtgtaccca aaaaacatta
    40621 caaggaaaaa ttgtcagtcg cagtggaaga aatgattgaa tcaggtgtcg aaatgaatct
    40681 cattgcgacg tactatcgaa cactggaaga actgaaaaaa caaaatggaa aatggttttt
    40741 ccaagcctta ctatgcttgg aagtaggtgt caaaccaagc actatcaaac cttccgaata
    40801 tcaagcctta gaactgactt atgcaaaatt cgcacagatt aagaaagcta aagcaatggc
    40861 tagcgactgg ctagatactt ttgaaagtat cgaaaaaaat ggtgcatttt atacgattga
    40921 atcggaggag aaagataatg aataaggtgg tttatcagct aacaacagaa gatgtttcgc
    40981 aatttttaac aagcaaagaa cacttgaatg aagtaatcag acggattcaa aaagacttta
    41041 gttgtatgga agaaatgaag tactttattc aaagagtgga agaacagttg tatatggaaa
    41101 gcgaaacaag tcaaacgaag gtcacagtca gccataatca tttagacgtg tataaacgtc
    41161 gtcctgaaac ggtaaaaaaa ggaacagagc ttctacgact agaaacagaa aaatacatca
    41221 tgaatgtgta cctagaaagg agtgagctgg tcgaaaactt tgtcagctac acgatttgta
    41281 ggaaacacta ttactcagaa tcagaatatt attcaatttt cgagagcatg gaattaatgg
    41341 aagaagaaat ggaagagcta aacagcaaaa atgcgacggt agacgattat taccttgtat
    41401 ttactaggtt tttgacgtgt gggacggaat ctatcgtgtt aatgggaatg gattatgaac
    41461 tcgctgtacc cgaagcattt ttgagggaat atgtagaaga agtagataac ccaggcgacg
    41521 actggtacac gactttaact gattttttag acacctacac ttacgacgat acagaagaaa
    41581 tccaaaatga acttgaccgt aaacgaatgc catatcaacg atttgaaagg aaaaaaggta
    41641 aataaccatt tataccgaaa agtgaggatg tgtgaaataa gaacaaaaaa ccttgaaatt
    41701 aagggaatgg ttcttatttc gcaccaatcg ctccaaccgc ccccgccgct taggtggtgc
    41761 gaaataagcg ggcaaagccc gataacctaa gagaaagaag gagatccata tgaataaaat
    41821 cattcctcta ttgttcgtac tgtttgttgg cgagatactt atgcgtcatg ctgcagctgt
    41881 acatttaaac ttcgtagtat tcttaggaat actggtattt tgtttgatct tttggcgatt
    41941 tgtattagcc cttgtgagtc tactattcaa gatgattgca gccatatttt tcatagggtt
    42001 atttcgatga aaggagagag tactaatgcc aattgcaata ggaaataaac ggttacccgt
    42061 gacattagat gaaaagagac aaaaagaatt gcagcaacta aagcagaagt acggaaaaag
    42121 tgaatctaag attatgtgta ttgcgttaga tctattgatc gcgcaagaaa aagcaggatt
    42181 tgatatacca gcactaaaaa agtgacgtca cttttcattt gaagaaacaa aagtgatagc
    42241 acttaattgt tcctactatt tagaatattt tctatgtcct ttttattcca ttttgtatca
    42301 ataactatga aatctttaaa gtatccagaa gagattattt ttagatcaat ttttttctta
    42361 cctgattcta aagattttgt tttcttaaac atattatgat ttcctttcga ctattttcat
    42421 ataaatgtta acatagttta tggattcatg gaatgttaat ctaatttaat agaaaaggat
    42481 aattaagtat atacgttttt tcttatgtat gttatactat gtatatacag aaagaggtgt
    42541 ctactatgga aatgttacct ataaaaaaat ggggaaatag taatggcctt agattgccaa
    42601 agtatattat ggaatatctt ggaattcata cagaagacaa agtgaagata atacaagaag
    42661 aagtaaacgg tcagaagcgg ctaattatcg aagcggctaa tatagaaaat gaattaacaa
    42721 ttgaacagtt atttgaaaat tacactgatg aaagaaatca tgtgacgatt caaaatttag
    42781 gtgaagcagt aggtaatgaa aaatggtgag aaaacctaga caaggcgata ttctattatt
    42841 gaatacagca cctcgatctg gacacgaaca gacaggtaaa agaccttata ttgttttaag
    42901 tcatgatatt attgcggatt atagtaacgt tgttacagtt gctccaattt ctagtacaac
    42961 tagaaactat ccgctatatg taaatataaa ctcagaatat gaaatgaaga caacaggaaa
    43021 agtattatta gatcaactaa cgacaattga ttatgaagcc agagagtgta tttttttgga
    43081 aaaagcgcat gatagtttag ttgaggagtt attgatgaaa gttagaacag tttttcaaaa
    43141 agtaagcaaa taaaatagaa acagcagcat gatcaactat tcgtgattga ccatgctgtc
    43201 tttttttatt ctatatacgg tttgtctcgt aacattgtag tcttttgcaa tcttagcgat
    43261 cgcaacacct ttttttagat cctcaacgat gtttttataa acaagacgac gttggggatc
    43321 ttttgcattg gcactgtata gcttaggacg tcctttataa acaccgttcg cttttgcaat
    43381 cttaatgcct tgtgcctgac gtcgtttaga ttcggtacgt tcttgttcag cgatcatggc
    43441 caaaatttga atgatcaaat ctttaatgaa tttatccagg agaggattgc caattgcttc
    43501 ggccatgatc ggtagacttg tgatgatcag ctgcacattt ttcttcttca agtaattgac
    43561 tgaagcgatg atctcgtcgt aatttcttcc aagtcgatca atagcttcca caataaaaat
    43621 atcttgatct cggacgaaat ccagtgcttc ttgaaaaaca agtcgatgag taatcgttgc
    43681 gccagattgt ttctcaatga aaattctctc tgcaccgaat tttttcattt cttcaatctg
    43741 ccgttgttca ttttgatcga tggatgagac acgtatgtat gcaactctca aaatttttac
    43801 ctactttctg caactattta aaaaacaccc taatcataca cgagaaaacc agatttttca
    43861 atgtcgcggt cgtgtctagt tagggtatac cttatataga caaaaactaa tgatggagga
    43921 aatagtatgt gtcacttttc tttttaaatg gcaatatttt ttgtaaataa atgtcaatca
    43981 agaaaattta aacgattatg acattaaaaa tatattttat gaattttcta gtatataact
    44041 tgatttatct gttaagattt attaagaaaa cgttttctta tttttgattt cgaggtgata
    44101 gacaatgaaa aataagttta ttttatcttt aggtatttta actattggaa ttagtgtaac
    44161 tggaactaca attcctacat ttgctgatag tacaaatgaa ccaactactg tagtagataa
    44221 taaccaagaa ttaactcctt tagataggaa cggaatagcc attggtaacc caactaacga
    44281 cttaactgtt actgaaaatt atttaatagt ttctccatta cttgctcgag cagcttctaa
    44341 aacgatacga gtgacaggaa cgatgatttt tgataaagtt ggttggcctt ctagacctcc
    44401 tcaatcacgg aattggaaag aaaaaagaaa tggttattgg tactatggaa aattgtattt
    44461 acaaagttat aaatcagctg gaaacaattg gattgcgact tattcaggaa ccttaactag
    44521 aaagtaggga ttttttatgc cagaatttgt gaacacacca aatttttatc aagatagcga
    44581 tattctaagt aaaattaatt taccgaaaga agaatcagct ttacaagttc agcgaacttt
    44641 acaggagaca caaaaaattc aaaaatcaat ttctaatcta gaagattctg aagaaaaaag
    44701 aaaaaaaaga gaccaaaaaa catatcaaaa agaaaaataa tgattttaaa aaaagagtgt
    44761 ctagtcgaca ttcttttttt ctaattgaaa gtgttcataa aatcacccat ttctggacac
    44821 tttctttttt tgctttattt ttcaaaaaaa caattcattt catgttcata aacccataac
    44881 aatatctatg ttcagttaaa ctatatggat aaagttatgt tacaataaaa ttaatgaagg
    44941 agtgattttt ttgaaaatag gatacgcccg ggtatctact ggtttacaaa atttggattt
    45001 acaagaagac agcctaaata aatttggttg tgaaaaaatt tttactgatc atatgagtgg
    45061 ggcaaaaagt aatcgaccag gattagaaat ggccattgat tttgttcgct ctggcgatac
    45121 acttgtcgtt tggcgcttag atcgtttagg tagaaatatg gaagatttga tttcaatcgt
    45181 caatcgactg aatgagcgtg gtgtgagttt tcatagtatt caagaaaata ttacgatgga
    45241 caaatcaagt tctacaggac agctcatgtt tcatttgttt gcggcatttg cagaatttga
    45301 gcgtaatttg atcctggaac gatccgcagc aggacgagca gctgctcgtg caagaggacg
    45361 gtttggtggt cgtcctgaaa aattgtctga acaagatttg gaattactga agactttagt
    45421 ggatagcggt acacctatta agactattgc ggaacggtgg aacgtctcaa gaaccaccat
    45481 ttatcgttat ttggcaaaaa tggaggagaa aaatggaaag acaaattctc attagttttt
    45541 gtcatttaat gctgaaaatt tttgccattt atctagaaaa gtgacaatag tatgagaatt
    45601 ttttgtaagt gtctactgat aacttattct caaatgttgt aaggatttta ttcatttgtt
    45661 aatatta
//
LOCUS       DAMEET010000008        43634 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.008, whole genome shotgun sequence.
ACCESSION   DAMEET010000008 DAMEET010000000
VERSION     DAMEET010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 43634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.008"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..153)
                     /locus_tag="PB780_000413"
     CDS             complement(<1..153)
                     /locus_tag="PB780_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDE0732488.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTS"
     gene            complement(194..439)
                     /locus_tag="PB780_000414"
     CDS             complement(194..439)
                     /locus_tag="PB780_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDE0732489.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDLMDRLQLTRQKN"
     gene            complement(538..852)
                     /locus_tag="PB780_000415"
     CDS             complement(538..852)
                     /locus_tag="PB780_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732490.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            complement(920..1414)
                     /locus_tag="PB780_000416"
     CDS             complement(920..1414)
                     /locus_tag="PB780_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732491.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            complement(1434..1721)
                     /locus_tag="PB780_000417"
     CDS             complement(1434..1721)
                     /locus_tag="PB780_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732492.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGVFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNK"
     gene            complement(1937..3328)
                     /locus_tag="PB780_000418"
     CDS             complement(1937..3328)
                     /locus_tag="PB780_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDE0732493.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            complement(3313..3924)
                     /gene="mobC"
                     /locus_tag="PB780_000419"
     CDS             complement(3313..3924)
                     /gene="mobC"
                     /locus_tag="PB780_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HDE0732494.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            complement(3928..4125)
                     /locus_tag="PB780_000420"
     CDS             complement(3928..4125)
                     /locus_tag="PB780_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732495.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            complement(4116..6794)
                     /locus_tag="PB780_000421"
     CDS             complement(4116..6794)
                     /locus_tag="PB780_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732496.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGF
                     KTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKSR
                     RRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGLSCN"
     gene            complement(6781..6999)
                     /locus_tag="PB780_000422"
     CDS             complement(6781..6999)
                     /locus_tag="PB780_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732497.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            complement(7075..8127)
                     /locus_tag="PB780_000423"
     CDS             complement(7075..8127)
                     /locus_tag="PB780_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDE0732498.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKSKMQMFPNFYYLLKPVLCKDDVYKINKITHQENRQKNLIVQNLNYGT
                     NQYSVDNSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLLESLS
                     TKNNKLDEELIETFIEENLLKQIWGESIVNCLKLASNSDYRQFDNWYKKFKYAIRSAE
                     KEQKIQLKIIYEICNNKYFVDHVREQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNK
                     ALELIELQISEGIDKQ"
     gene            complement(8280..8606)
                     /locus_tag="PB780_000424"
     CDS             complement(8280..8606)
                     /locus_tag="PB780_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732499.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            complement(8618..8818)
                     /locus_tag="PB780_000425"
     CDS             complement(8618..8818)
                     /locus_tag="PB780_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDE0732500.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKI"
     gene            8941..9543
                     /locus_tag="PB780_000426"
     CDS             8941..9543
                     /locus_tag="PB780_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDE0732501.1"
                     /translation="MEFTIGGVTLNRIKFYIDSSKKTIKQISTETGINYTTLTNYYRG
                     ERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIKTARLEKKDKIVKNIAS
                     LDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSFEYTILFAKQKKFINKA
                     LNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            9598..10764
                     /locus_tag="PB780_000427"
     CDS             9598..10764
                     /locus_tag="PB780_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDE0732502.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            complement(10853..11245)
                     /gene="rpsI"
                     /locus_tag="PB780_000428"
     CDS             complement(10853..11245)
                     /gene="rpsI"
                     /locus_tag="PB780_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDE0732503.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(11261..11704)
                     /gene="rplM"
                     /locus_tag="PB780_000429"
     CDS             complement(11261..11704)
                     /gene="rplM"
                     /locus_tag="PB780_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDE0732504.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(12223..12690)
                     /locus_tag="PB780_000430"
     CDS             complement(12223..12690)
                     /locus_tag="PB780_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDE0732505.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(12810..14348)
                     /locus_tag="PB780_000431"
     CDS             complement(12810..14348)
                     /locus_tag="PB780_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDE0732506.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            14428..15114
                     /locus_tag="PB780_000432"
     CDS             14428..15114
                     /locus_tag="PB780_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDE0732507.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(15315..15842)
                     /gene="msrA"
                     /locus_tag="PB780_000433"
     CDS             complement(15315..15842)
                     /gene="msrA"
                     /locus_tag="PB780_000433"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDE0732508.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(15926..16603)
                     /gene="pgmB"
                     /locus_tag="PB780_000434"
     CDS             complement(15926..16603)
                     /gene="pgmB"
                     /locus_tag="PB780_000434"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDE0732509.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(16600..17730)
                     /locus_tag="PB780_000435"
     CDS             complement(16600..17730)
                     /locus_tag="PB780_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDE0732510.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(17730..19913)
                     /locus_tag="PB780_000436"
     CDS             complement(17730..19913)
                     /locus_tag="PB780_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDE0732511.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(19910..20935)
                     /locus_tag="PB780_000437"
     CDS             complement(19910..20935)
                     /locus_tag="PB780_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDE0732512.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(20980..21807)
                     /locus_tag="PB780_000438"
     CDS             complement(20980..21807)
                     /locus_tag="PB780_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDE0732513.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(21804..22871)
                     /locus_tag="PB780_000439"
     CDS             complement(21804..22871)
                     /locus_tag="PB780_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HDE0732514.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(22897..23733)
                     /locus_tag="PB780_000440"
     CDS             complement(22897..23733)
                     /locus_tag="PB780_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDE0732515.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(23730..24599)
                     /locus_tag="PB780_000441"
     CDS             complement(23730..24599)
                     /locus_tag="PB780_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDE0732516.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(24669..25952)
                     /locus_tag="PB780_000442"
     CDS             complement(24669..25952)
                     /locus_tag="PB780_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDE0732517.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(25966..27651)
                     /locus_tag="PB780_000443"
     CDS             complement(25966..27651)
                     /locus_tag="PB780_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HDE0732518.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            27926..28951
                     /locus_tag="PB780_000444"
     CDS             27926..28951
                     /locus_tag="PB780_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDE0732519.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(29546..33220)
                     /gene="rpoC"
                     /locus_tag="PB780_000445"
     CDS             complement(29546..33220)
                     /gene="rpoC"
                     /locus_tag="PB780_000445"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDE0732520.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVNVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLEENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(33278..36895)
                     /gene="rpoB"
                     /locus_tag="PB780_000446"
     CDS             complement(33278..36895)
                     /gene="rpoB"
                     /locus_tag="PB780_000446"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDE0732521.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            37623..38591
                     /locus_tag="PB780_000447"
     CDS             37623..38591
                     /locus_tag="PB780_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDE0732522.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            38609..39514
                     /locus_tag="PB780_000448"
     CDS             38609..39514
                     /locus_tag="PB780_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDE0732523.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            39511..40317
                     /locus_tag="PB780_000449"
     CDS             39511..40317
                     /locus_tag="PB780_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDE0732524.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            40453..41442
                     /locus_tag="PB780_000450"
     CDS             40453..41442
                     /locus_tag="PB780_000450"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDE0732525.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(41759..42283)
                     /locus_tag="PB780_000451"
     CDS             complement(41759..42283)
                     /locus_tag="PB780_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HDE0732526.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            42570..>43634
                     /gene="scm"
                     /locus_tag="PB780_000452"
     CDS             42570..>43634
                     /gene="scm"
                     /locus_tag="PB780_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HDE0732527.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT        12499 a         9070 c         7667 g        14398 t
ORIGIN      
        1 actcgtttta gattgcttta cccatttgtc aaatgctgaa gccgttagtt catattctcg
       61 aatgatttct acacgtggct ttccagctaa gtaaagattg acgatttgtt gcttgaattc
      121 ttgtgaaaaa gttcttcgtg ttctcttaga cataaaaatt cctcctggta tgttttcttc
      181 tagtctacac accttaattt ttctgtctag ttaattgtag cctatccatt aaatcagata
      241 cataagccca tgtacccgtt cgttgtaaat aaagttcttc ttgtaacaac tcccattttc
      301 tcttagaaat cataatgacg ccatcgttca atccattttt agaatcccta gtaatttcta
      361 tttcagaatt tgaatgaact acttcttgca acactttacc tatattttct ttgaagtttg
      421 ttggagtaat agtatccaaa atgaatacct ctcttctcta tacctctatt atgaagtgcc
      481 cataaaataa agcaagcgtt gaggaataat tctatcaaca cttgctttat taagctttca
      541 ttttgtagtt tgactaaaac caataatctt atttctaaac tctcgaaaaa cttcttgtgg
      601 ttgataatag tctggatcta tttcaaatgt ttgtgagaat aattcattga atttttctac
      661 tgaaataatg attttaccat tcttctctcg actgttttct attatttctg cttctaattc
      721 aaataaatct agtgacgaac taaaatcata aatcatgtca tattgaataa aaaaatcaag
      781 aaaaagttgc tcaaactctt ccttatttat cactaatgtt tcaaacggaa caactttatt
      841 ttcctcagcc attctcccag ttccattttc aaaaatcaca aaactgatat taccttaaaa
      901 actactaaca aaataaaact tataaagtaa atggcaaccc aactatgtaa ccattctctc
      961 tacgtactac gatttggtag tcctgccttt caatcggtaa ttcataatag taatttactg
     1021 tttcttcttt gccactttca atatactctg gattatccaa tatcatatca tactcataaa
     1081 gttcttcacc atccttattt tcaatcgacc attgttgtgg acgtatcgga ttttctgtat
     1141 catttataag tgtataggtc acttgtaata cacgatcatg ttgtgcaaat tcatttcttt
     1201 tttcggtcac ttcaatattt ttaaatgtta gttcaacccc atctgcctga taagtttcac
     1261 ctatttttaa atcttttgtt acaattttta ttggcttttc ttctggaaaa tgtctggctt
     1321 taattgttag ataacttcca aaaagaatca atcctagtag cactaaaaga attcctttaa
     1381 cgagccactg aaacgggaag taaactttaa acatcttttt tctcctccgt tttttatttg
     1441 ttttttagag aatacgataa cttttgatca atgtcatcca gcttttcaat tacttcatta
     1501 cttatcattg attctagttc tgaaattttt tgttcaaatt taatgctttc aggatcatta
     1561 aatttattat ttgatggact agctaacact ggtaaattcg aaaccataac acgaaacata
     1621 tttgcctttg aaccaaaaac tcctttgttt tggttgtaaa aattgttgaa tatttcttta
     1681 gtttcttctg aagccgagac tttaaaatat ggatagttca tttttttctt ccacctttct
     1741 ttttttggga gggagtctgt agggagcttg ctccctcaga gatttaccca ccatctgact
     1801 tgccagaaag tagcctagcg tgagcggggc ggattgactg gcaagtagat gggtagctgg
     1861 cgtatgtcct aaaaactgct tctttccaag caactcttca taggcaaaaa actggaaagt
     1921 ccactaacaa gcccattcag aattccattt caaaattttg ttgccttttt tgctgatttt
     1981 caatcatttg ttgtgtgaat tcataatcct ttttagcacg atacattttt acatcgtcat
     2041 tcattatgcg actgatttga tagtgaatct gtttagcaga aggtgtatga gaacaatttt
     2101 caaaaccctt tgccggtaga aataactctt tctgatcagt ctttttaata gaaattgttt
     2161 tacttttcgc actacgtaaa gtttgaggat tcacatccgt atgaagttta atcgtgtatt
     2221 cttgatcatc ttctaaagac attgtattta caaaatgctt gtctacctct cgcaagaagt
     2281 catcatattt aatgaatttt tcatcgcctt cacggtcgct aaattgcttt tgtgcgatgg
     2341 aatctaattc cacaaacggt acatttaact caccagtttt gtaatttact ccactaatta
     2401 gcgacataac ttgctctctt tccatattaa aaaaattttt tatatgtgat tcttctaaat
     2461 tcttttcaag ttgttcagaa acagattcaa atgaatatct tttccctaac tttcttgcac
     2521 taaaagtacg ttcgacatac tgactttttt cttttctaga ttgtttatct cttcctaaag
     2581 taattaactc acctctacgc tcatagaaat ttacaccttg tttgtaaaga acatagccaa
     2641 gtttgttatt tgtttttgaa aattgataaa cttcaatgtt atattctttt gccaaatgat
     2701 ccgaaaaatc ttcaaaagaa ttcacttcgc atttttccaa cgttgcttcg atcgcatttt
     2761 gcacaaactc ttgataatta atttttccat atttcatctt agaaggatcg tttgaataaa
     2821 gattggttct cttttctaag ttttcatagt actcattatt cactgattca agaccatgtt
     2881 cattgaggac ttgatcgtta atttttcgca ttagttctaa atctttaggg tcaatacgca
     2941 accttttaca tgtttccatg ttaatattat taatgacaag atgactatga aaatgatctt
     3001 tatcattgtg acttgcaaca atgatttgcg aatttggaaa agctttctct gcaaattcga
     3061 cagctatttc gtgcatttta tcaatactta actctttaga agtactctta tcaaacgaat
     3121 gaataacatg aaacgcttgt gctatatctt tagatttatt catcttggta cgtactaaat
     3181 tcatttcttg cagtgcttcc tcactatcat cactacaatt tattcctgag actgcaacac
     3241 atttttcttt ttttgaagga ttagctgcat attgtaatag cgaggataaa tttttcttgc
     3301 gtcctttcga tgttatagta gctcccaaag ttgtttcacc tcatctttta aacttgcaat
     3361 aatctgaatt gagtgaatta aattccgatc aactttttgg gaatactctt ctttcgtacc
     3421 cggtctagtt tgcttttcta tccaatattc gaaatattct ttttctactt ctgataattt
     3481 aaaaaaagag ggggcactcc gattggactc caaaaaaatg tcatctggat aactaatcat
     3541 caactttttt gctatgctat tcgcaagttc atctttaaaa atgttgtaac ggctagcatc
     3601 aaaactaaaa tgtaggctct tttctttttt atcttcagat tcttctctaa aatatttagc
     3661 aatgatattt aatgccttta ccgcttgatt caaattgtta ccaatttgat ttaactgttt
     3721 ggttaaaact accctatcat cttcagctaa caacggcggt tcatatttga gtccctttaa
     3781 ccgctcacgg caaacgtctg ctctagaaat tcccctttgt tttgcaatca atgtcagttc
     3841 aagataagtc tttttatcga cacgaaaatt taaggcctca ctatgtttgc gatttttttc
     3901 gtacattttt tgtttatcat ccattgatta tcccctcaca tttttttcta gagagcaaca
     3961 tttgaaattg ctctctattt catttttcat ctttaattct tcttgaatca tcagttcaat
     4021 ataattagtg accgtgattt tttcatttgt tgcgttcttt actgattcac ataattcgtc
     4081 aagttctaag aaatcatgcg tactaatctt tagaattaat tgcatgacaa tccccccctt
     4141 ttatttctta ataaattatc gaaaagtgga ttaggatcat taagtgtatc tctgaataac
     4201 tcattgtctt cataagtgaa tcttcgtctt gattttgatc ccttatcatt ttcacggatt
     4261 tcactatcaa taatcactgt ttcatgattt gaatcaaatt ttttcgctga ctttctgtct
     4321 tcaacgatat tgattttttg tgttttttct ttcatttctc cactatcttt ttgaatgttc
     4381 tcccgaacat ctactgtttt gaatccttga ctacttgtag aactttgtac attttctcgg
     4441 acatctactg tttttggttg acttgcactg ctaccttgca cattctctcg gacatccact
     4501 gatttaaacc cttgactact tgtagaattt tgtacatttt ctcgaacatc tactgttttt
     4561 ggttgacttg tactgctact ttgcagattt tctcgaacat caattgtctt agtaccttga
     4621 gcatttgaag aactttgtaa attctctctt acatcaacat tccgtccaac atgaccattc
     4681 agtgaacctt gaatattttc tcggacatct actgatcggc caccatgact acttgtagaa
     4741 ttttgtggat tttctttgac taatttttct aatcctgtat ttgaattttt agcttcttga
     4801 attctttctt tggtcaacgc ttcttttccg tcaatattag cgcctgtccg cccttctaca
     4861 cgtttcctta acattatttc ctcgcgattc attgcttcag gcgtgtattt cgcttcatga
     4921 cgcttctgat taattaattc ttcagcagac ttagcttgac cgtgcatagc atttttacgt
     4981 tcttcaatat cttgttggac tttttgctga attgcgcttg caggattatt attcgattca
     5041 gcattatttt tcattgcttc acgcatacgt tcagaactag acataattct atcggcattt
     5101 tcaactttag caccatcttt tctatctgaa agacgtgttg gcacggaatc agcatatttt
     5161 ccagtttcat cttctttatt tttagctaac ataggtttgc tatttttatt aatagcggac
     5221 actgtaccat catctaaacc ttctttaaac ttattcttgg tatcattagc tatatcacgc
     5281 atactcttaa atggttttgt tgctctttca ctagccattg tagctccatc tgaagctaaa
     5341 ccggataatc cacgttcatg ggcatatccc aaagctcttc cagttttgcc agcttttttt
     5401 gatagagttt cgccgaaatt cttttctggt tttcgatctt ctccttctcc gcttccttta
     5461 gcacgattaa atccatttga tccttttcta aacagcattg cgcccattcc aaaagcagaa
     5521 gcaagttgcc catagccttc tttgagtcca atatcaacac caaagtatct catgactgtt
     5581 ccactgcctt tgattaaaac aaaaacggca cagatataag caattgtttt tataaaaata
     5641 tttgttgaga taccttgtcc tgagttcaga aaattgataa acatagcaaa cattgataac
     5701 ccaaatcctt gaaacccaac agttaagtaa cattgtagaa tatctgatag aacaactttt
     5761 gaacgctgtc ctgtctcaat atctgttgca aaaacaagaa cacctaacac acgtttaaat
     5821 gcaagttcta gtattgctgt aatgatcaca aacgcgctga acacataggc gattgctaac
     5881 gctgtcaacc caattgaaat cccccataga tttgcttggt atcggtaata gcctgatttc
     5941 aagctatcag aaaatgttga taagaagttg tcagaaaact ttgtagcaac aaattcatta
     6001 ttagaatttt caactaattc ataacgaaga ttgtccgcaa ttggattttc tttttccaat
     6061 ttatcaatca ctttatccgt taacacttgt gtgagatccg tctgttgaaa attttttcgt
     6121 gttaaattat ttcgatcctc cgttttatca atgcttgagt actgatctgt tttattaata
     6181 tatgctaaat ctgttactcc ttgttttatc aatgaccaag cgactccgtc atctgaattt
     6241 gtaatcgtag tagagtcctt ataaaatgtt ttagcaaatt gaataccact agaaatcatg
     6301 gttggcatta aaagtattaa tgctatagac atcactacgt tcattccaac cgattttaaa
     6361 tccactgttc cttttccagt aatcattttg taaccaacaa acatcaatga aaggatcatt
     6421 aatccaatga caattgtatt tacggctgat tgataaacat tatttagccc tgtactttca
     6481 atgaaattga acaaatccag cacatctgga atcatatttt gaatcgaatc tgtcacagta
     6541 aacatgcctt ttaccacagc ccatccgagc caaatcccga actgactaag gaaatctcca
     6601 ttgtactttt ttaggtaagg tctataaaga gaattattca aaaactctgt ttgacttttt
     6661 gtgtcatcaa accaattgaa aatactcgca tgagcaatat tacttgtaaa aataatcata
     6721 gctatcagaa caagaaaatt aacaaccaac caatttattt ttttattggc aatctttttt
     6781 tcaattattt tcatcgttac ctcccttagc ccattttctg aggctctcag agagcttctg
     6841 agccattttt tgattaatta tgacatgagt tacacaatta tcattttcag ctcttaaatc
     6901 gagaactata gtgttgaagc aaatagtctc gattaattcg agactatctg tattttgatc
     6961 tagacaccaa acttgatcta ttaaacgatt tttaatcaaa ataacccctc ctatattcaa
     7021 agttctcaaa tgaagtggag atacccctcc atttcatttg actcaattct attcttattg
     7081 cttatctatt ccttcgctga tttgcaattc aattagttct aacgctttat tttttagaga
     7141 agcaaagata taatcgtctt tttgtttgat acgatgatct gtttttgctc ttcgtatcaa
     7201 gtcacgtaaa gtcattgata gctgttctct aacatgatct acaaaatact tgttattaca
     7261 aatttcatag ataattttca gttgaatttt ttgttctttt tcagcagatc taatcgcata
     7321 tttgaatttt ttgtaccagt tgtcgaattg acgataatca ctattgctag ccagttttaa
     7381 gcaattcaca atactttctc cccatatttg tttaagaaga ttttcttcaa taaatgtttc
     7441 gatcaattct tcatctaatt tattattttt agttgaaagt gattcaagaa gatcatcatt
     7501 atgtgattct ttaatatctt tactatcttt ataagactta tataattcta gttcattttt
     7561 tgaactatca gcaaaattgt ttttgtctct tgttttcatt gatgtgtcgc atagttcaaa
     7621 ttttgaatta tcaacagaat attgatttgt tccatagttc aaattttgaa ctatcaagtt
     7681 tttttgtcta ttttcttgat gggttatctt attaatttta taaacatcat ctttgcacag
     7741 aacaggcttt aacaaataat aaaaatttgg gaacatttgc attttacttt tattgaagcc
     7801 attttttctt ttcacaagaa gtttaacttt ttgaagttca tctagtgctg ataagattgt
     7861 attttttgac attcccgaaa tttctacgag atttttgatt tcaaatatca aaaaaatttt
     7921 ttgattctca tcatacaagt tatttttcaa tgaaagtcta tttctatctt ttagtaacat
     7981 ataaactaac ttagaactta gtttcatttt cttatatttt tcatttacgt ttaaaaaaat
     8041 aggatattga tagtataatt ctgcatatat atcagaatta gaatagcctt gcttttcaac
     8101 taagctttca acttcttttt cattcattgt cacacctgct tctgatttta tttgctataa
     8161 tagattcaaa taaagttttc tcagaacttt attattgagt gttcccccga tctgcaaact
     8221 gacgggaaca ctctttttaa ttttttctac ttcatcaaat ttaaaaaacg aggtgttttt
     8281 taaatttgat gactcaatat ccattttttg cattcttctt ttgaaaacaa ctccattttt
     8341 tctaataaaa taactggcaa tcccattctt ttaaatttca aaaaagttgt atatgataca
     8401 ttcaaatatt tagccatctc ttttcctttt agaaatggtc tttctaatcc ggtatcaatt
     8461 cttgcttgat caatagcctc ttttgtcatt tcaaaaacat aatttttcaa ttgattcgtt
     8521 tgttgtttat ctaacattac tgtaattcct gcatccatta tttttctcct ctcattttct
     8581 atttcttcaa tcagccaact cgccaagttg tatttattta gattttcaaa tcattaagta
     8641 accaatttac caacttttga taagtagatg ctctaatact ttctttgctc ccattttcga
     8701 ttttgacata tgttttccga tttataccta tcaattttgc tgcatcgttt ttagtaagta
     8761 taagatcggc tctatgtctt cttaagcttc taatgaattg atcattaaga gttatcattc
     8821 agcgcctcct ttcaaattat tcactttgtg gtttttatct taataagcac attttaaacc
     8881 acaaaacgag taatatcaat taaaatatta ctcaatttgt ggttttcatg ctataatagt
     8941 ttggaattta ctataggggg cgttactttg aatagaataa aattttatat tgattccagt
     9001 aaaaagacca ttaaacaaat tagtacagaa actggtatta actatacaac tcttacgaac
     9061 tattaccgtg gtgagcgaaa tcctagaaat actgaaacat ggaatatcct tgctgattat
     9121 ttccatgttc ctgtaagtta tttgatggga ttagacgaaa caaattccat agaacaacct
     9181 aatcttgata gaataaaaac tgctcgttta gaaaaaaagg acaaaatagt caagaatatt
     9241 gccagtcttg actcagatta ttttactgaa ttatctaatc tatacttttc catgacttct
     9301 ttacttgaag tcaaacttca agaagatgac tccgactttc tttttttaaa aagtattcta
     9361 gaggactcct ttgaatacac gattctattt gccaaacaaa agaagtttat taataaagct
     9421 ttaaacttaa atatcgatga aagaaaagag ttagaatctg agttatttgg caacttaacc
     9481 caagtacagg ttaacaatct aaaaagagag ataacagctc gattaatatc aaaacttcaa
     9541 taaaactaat tattcaatca gccaactcgc caagttatca gattggagag attagttatg
     9601 tcaatacaaa agtacacaac aaaaagcggt gcacgatata gtgtccgctt gtatgttcgt
     9661 aaagatgaaa atggaaagca agaatactat gtaaaaaaag ggtttaaaac tgaaaaagaa
     9721 gccaagttac acgaagcaag aaaaaaggtc gagattgaag ataccggcta tcataagcct
     9781 tctaatgact tgtttataac agtgtatgaa gaatggttgc cttcttatag gaatcgtgta
     9841 caagaaacga catatcaacg tacaaaagac cttttccgct tgcatatact accaaaattc
     9901 gggaataagt ccatttctaa gattactcct gttcactgtc agaatgcagt caatgaatgg
     9961 gctttaaaat tcaagaatat caaacagctt aagtcttaca gctctcaaat atttgagtat
    10021 gccatttttg cagagctatt acaccgaaat cctatggcaa atacaaatct accaaagcgg
    10081 gaaaagaaag aaaatgaaaa ttacttttca ttagaagaac taaaagcttt ttgggatatt
    10141 ctcaataaag aagagccttt aaaacatatt ttaatcttcc agcttttgat tactactggt
    10201 atacgtaaag gagagctagc tgctcttcat tggtctgaca tttattttga agaacagcta
    10261 atgtatgtta gaaagagcta tgcaactatt cgaaatgaag agaagaatgc aagaagaaaa
    10321 actgtcagga ttcaaaaaaa tacaaaaaat atatcttccg aacgaatatt accaattgat
    10381 gcacaaacta ttgaaatgtt aaaaatctgg aaaaaagaac aagctcgaga gcttttgcag
    10441 tttggcataa atacaaatac aaaaaatcaa cttatcttta cctatgtaaa tgcagacggt
    10501 gacattaatc aacctttgca tgcggactat tcaaataata tattgaaacg tttagagaag
    10561 aaatataaat ttaagcacgt tactattcat ggattccggc acactcacgc tactcttcta
    10621 ctagaaggtg gagcaagtat caaagaaaca caagatcgat tgggtcataa aaatgctgaa
    10681 actaccctta acacttattc tcatgttact gaaaaagctc aacgaaatgc gttagaaaaa
    10741 ttcattgctt acactggttt ataatattgt cattaccttt ctcattacca gaacgaaaaa
    10801 aagacacctt ctcgcaatga gaaagtgtct gaaatcgttg gtataacaac gattaacgtt
    10861 ttgaaaactg tgaagcttta cgtgctttct taagacctgg ttttttacgt tcgaccatac
    10921 gtgcgtcacg agtaagtaag ccagcacgtt taagagctaa acggaagtca ggatcaactt
    10981 ctagcaatgc acgagcgata ccatgacgga tagctcctga ttgtccagcg tatcctccgc
    11041 cgtttacgtt aacaaatacg tcatatgcgc cttttgtttc agtaacagcg aaaggttgat
    11101 tgattacttc acgcaagtca gcatgtggaa tgtattcttc aacatctttt ttatttacag
    11161 taattttgcc agttcctggt actagacgta cgcgagcaac tgcgttttta cgacggcctg
    11221 tgccgatata ttgaacttgt gccaatgaaa ttccctccta ttagattaag tttgtgatgt
    11281 ctaaaacttc tggttgttgt gctgcatgtg gatgttctgc tccaccgtat acgttcaatt
    11341 ttgtaaattg tttgcggcct aaagtgtttt taggaagcat accttttaca gaagtttcga
    11401 tcaaacgacg agaattttta gcacgcaatt cgccagcagt gattgatttc aatcctcctg
    11461 ggtagttgct gtgacggtaa taaattttgt cagtcgcttt tttacctgtt aatttcactt
    11521 tgtctgcatt gatgacgatg acgaaatcgc cagtatccaa atgaggtgtg aaagttggtt
    11581 tatttttacc acgtagtaca gatgcaacaa ctgttgaaag acgtcccaat ggaacatcag
    11641 ttgcgtctac tacataccat ttacgttcta cttcgcctgg tttggccata tatgttgtac
    11701 gcacgttatt ttcctccaat ttaagttctg atgtttgaca tacacttcga gtttccgggg
    11761 ctctttgtgg ggcaaacaat accatttaac atattacccg ctaatctagt caatgtcaac
    11821 gtttttctgc ttgattgaaa aatttatatg aaactttact tcttttcttt cttttatata
    11881 aaagggtcgt ttcctgtcaa aatgaaaaac aaaaagaggc tgtgacaaaa gtcttgtcac
    11941 aaccccttat tttgaataaa cggtgttctc ctgccaactc ctcggtcaca agtcacaata
    12001 tccataaaac ataagaagct gttttttaaa attgtttctt gtgactcgga gctgaacggc
    12061 aggcttacaa cctcttggta aacggtgttc aatcagctga ccctcggtat tagctcaaaa
    12121 aacggcaaaa tatgaggaac tattttgacg tttgtttctc taatactcag gtcaaaacgc
    12181 tgatttcaca acctcttttt cggtcaagtt caacaacagt ttttacgcgt cgatctctgg
    12241 cgctttgccc aaatgagctt ggatcatcca gatacgtttt tctgtggctg ttttgaattc
    12301 gataaagata tcttgtgttg gatcgtcgcc ttcttctcca gaaacttcga ttcctttttg
    12361 gtaaagatct gctagataac gatagcctgc aacaagtgtt tccaaacgtt ctggaatagt
    12421 acgatcccag cggccaattt catcagggat ttttgtgtta tctgcaaatt cacgcaatgt
    12481 agaataaggt gaaccgtcta atgtgatcaa gcgttcggat acttcatcta actgagcgtt
    12541 caaatcatcc atgaactcat ccatcattgg gtgcaatgta aggaaacctg gtcctctcat
    12601 gtaccaatgt gtttggtgaa caacaacaga catttggctt agatctgcaa ctaattgatt
    12661 taatacttct ttggtttgtt ggaatttcat ctttcttgcc tcctaacatt ttcttacact
    12721 taaaataata gtagactttc aatgaaaagt acaacaaaat catttacctg agaacgattt
    12781 tcagttcgct tttttatccg taaagccaat catttttcag aatcagctaa ttcttctcgc
    12841 agttgagcaa attgctgata agtctgtgct aaatgctttt gtgtcgaaac aaataatgga
    12901 tagattttct gatatttttt atgatcagtt tcttgtactc caacttgctc tttcaattct
    12961 atcaattcct gtgcttcact gatactttgg atctttccga tgcttctcca gcctaaaatc
    13021 acagctccta atgcggatgc ttctgtgaca gtagggaaaa caagcgggta acccagtaca
    13081 tcagccatca tctgacgaaa aatcggagat tgagcaaacc cgcctgtagc acggatctct
    13141 gtgagctgat tttcctggca aactcctttt aaaatccgat aaaggttgag acagatccct
    13201 tctagaatcg ccttcatcat ttctttttct gtatgttgcc gctgtaaacc gataaaactg
    13261 ccaaacgcat ctgcgtccca aagcggtgct cgctcaccaa gtaaataggg atgaaaaatc
    13321 agaccattgg cacccgctgg cacaaaagcg atagccgaaa gcagtgcatc atatggattt
    13381 tctccttttt ctttagcaga ttgaacgact tcctgtaaaa atgtatgccc tgcccaatca
    13441 aagatgccag caccattgct agtcgctcct cccactaccc aatgtttctg atccagaaca
    13501 taacaaaacg tctccatttc tggatggaac tgaggctgag tagtaataaa acgaagtgct
    13561 ccgctcgttc ctacggtcaa agcggcgatc gtatcaccaa aagcacctaa ccctagatta
    13621 gaaagcggcc cgtctgcccc tcccaaaaca aatgatatat catttggcaa acctagttct
    13681 gctgctgttt ttgatggtag acctgttact tgataggtgg aaggaaagac ttgcggcaat
    13741 tgctctattc gaatgttcaa gtagtcaagc acttcttctg cccattggaa ttcatggata
    13801 tcaaaatatc cagttcctga agctgttccg tagtcgctga tcagttcgcc aaacaatcta
    13861 taaaagatat agtctttgat accgacaaaa taagcagttt ttttgaataa ttcaggttgg
    13921 ctgttttgca gccacttgat tttgttcaat ggtgtcattg gatgaatcgg cgtcccagtc
    13981 tgccgataaa attctttgcc aagcacggta tatttcagtt tttctgcttc gtccgctgct
    14041 cggttatctg cccaagtgat cattcgagat aacggtgttt tctcttcatc caacaatatc
    14101 aggccatgca tcgcagagga gaaggagaca aattgaatga ttgtgtctga aggcaactgt
    14161 ttaattactt cttttataac ggtgcagaca ccttgaaaga tcaattcctg atcttgttct
    14221 gccatccctt gtgtttcttg gatcaaaggg tagcgaaaat aagcggatgc tgcttcttgg
    14281 gcttcatctg tgaaagcaac tgctttggtt tgtgtggttc cgatatctaa tccgattgaa
    14341 acgttcattt tttcacctca aattttatgt atgtacaaaa atttagttta agcttgcgta
    14401 tcttggtttt gaaaaggggg agatggcgtg ttactttatt ttatcattgg ttgctgtatc
    14461 ggatcatttt tatgtttaac cgcccagcgg attccgttag gtcactctat tatttatcct
    14521 agatctcatt gtgtcaaatg ctgtcgttcg ctttcttggt atgaattgat tccgattctc
    14581 tcgatcatcg tccaacgatt tcgctgccgc tattgcaggt gtcggttgcc cttctactat
    14641 ttgttggcag aagccttatg tggtggtctt tttgcctggt tctttacttt ttcttccgca
    14701 agaaattttt cgacgttcat atggatgctt tcggctttgc tattttcgtt gatggattta
    14761 ttttacttca tggtccattc tcacacgcta ttctgttctt gggcaatttt atggattttt
    14821 tggttgcagg ccggcgtctt ccaatggcaa agtgtgctgt taacgtttat cgtaggcggt
    14881 gtgtgccttc gctatggtca atcctatctc ggagctggcg atactctgtt gatgctttct
    14941 tggagtggtg gcttgtctct agaagagttg cttcagatct tgtttcttgc tagtctgttt
    15001 ggaatatccg ttttttctgt ttactttatc tgtcataaaa aaaagttgga aaagctgcct
    15061 ttcattcctt ttttgagcag tgcgttgctg attgtccttc atcttaggta ttgaaaaaag
    15121 gttgtgacaa acgacttgtc acaacctctg atatatagtg ttctcttacc aaatccttgt
    15181 gacttggagc taggcgacag gttcacaacc tctccactaa atggtgttca atcagctgac
    15241 cctcagtatt agctcaaaat gctggtttta caacctctga tatacggtgt tccataagta
    15301 gctttttcgg cttcttattt cttgacgccc caatgtcgac gaaggaaacc tgcacgtcca
    15361 gaaccttctc gataaagatt gtaatggagc ttgtttttct tgtaataatc ctgatggtaa
    15421 tcttctgcag gataaaattc gcttcttggt tcgatttttg tcacgattgg cttatcgaat
    15481 cgcccgctgt cttgcaatgc ttgttttgaa cgctctgcaa tatttcgttc ttcttcatca
    15541 ttatagtaga tcactggacg ataagaatca ccgcgatcag cgaactgtcc taatgcgtca
    15601 gtcggatcgg tttgctgcca atagatttcc accaactgat cataagaaat aacttctgga
    15661 tcatattcga tctctactgc ttctgtatgc cctgttgtgc ctgttgttac ttgttcataa
    15721 gttggatttg gtacatgccc gccagtataa ccggatgtca cagaaatgat ccctggctgc
    15781 tcttcaaatg gtttgaccat acaccaaaag catccgcctg cgaaaactgc tttttttgtc
    15841 atataaaatc cccctttttt ctcatcatac cttgatttcc aaaaggagga taacgttttg
    15901 cttgtgacag aaaaaacgga aactattata tacgtgatac ggcttctgat aaatgccaaa
    15961 acgaacgtac ttgatcaaaa acggttaaat caggaatcac atattcagca gaaggaagat
    16021 ttatgttttc acctattccc aatacatgca tgttaccagc caaagctgct tgacaaccag
    16081 cttctgaatc ttcaatgacc aagcatgcat ttggcggtac attcaattgc tgtgctcctt
    16141 tgagaaaaac ttctggatca ggttttgctt ttgatacttg cgtgccgtca ataaggacat
    16201 caaaataact tgtcaaattc gttttttcta aaatgaggcg tgcatttttg cttgctgatc
    16261 ccaatccgat cttgatgttt gtttttttca agtactcaag aacagctatt gcaccaggca
    16321 aaacggatgt ttcatccatt ttttgaatcg cttgaagata tagattgttt ttttgctcag
    16381 caaaggctga tttctctttt tctgagaatt tctgttccat ttttccaaat gacagcaatc
    16441 gctctagtga ctcggttctg gatattcctt tcagttgctc gttttctttc tcagtgaatg
    16501 gaatggacag ctggttagct aaaaccagcc acgcttcgta atgataacga gctgtgtcta
    16561 caagaacgcc atctaaatcg aatagtgctg ctttgatttt catcgattac ctccttgaaa
    16621 atgaacgact tgaagtaaag atgcgatgtt tttagctgtg cgatgaagat tatcagtggc
    16681 ttgctgtaaa gcttcttgta aaggcacagc tgcaggaagg atcggaaaaa cggccgaaat
    16741 gccaacatct gctgcttttt cgcttccttt accaacactt ccgcaaatag ctatcacagg
    16801 aacgccctct ggtactcttc tagcaatccc aactggcgct tttcctttta gactttgtcc
    16861 atctagacgt ccttcgccta cgattacgag gtctgcttgc tgtacccgtt tatcgaactc
    16921 cagtaaatcc aatacgtagt cgatgccaga gactattttc cctgaagcaa atgctaacag
    16981 tcctgcaccc attccaccac cagcactact gcccgatgcc tcgattactt gaggggcaaa
    17041 tctcatataa aaatcatgca tggcttggtc agccactgct aattcatttt ctttcaagcc
    17101 tttttgtggt ccgaaaacaa aagaagcgcc ttcttttccg cataaaagat tagtaacatc
    17161 agtaatgaca gtgattttgg ctttaatcag ctcaataggc acgttcgttt tatcaactgt
    17221 agcaatttta cctagatttt ctcccagtgc ttctactttt tgattatctt gatcgtaaaa
    17281 agcataacct agaccttctg ccatgccgat gccgccatcg ttggtactac ttccgccaac
    17341 gccgatgatg atgtgtcgaa caccttgttg caacaattgt cgtatcagtt ctcctactcc
    17401 tcgcgtggat aacctaagtg gctgttgttg gtttagtggg acatctttca atccgacgat
    17461 ttctgccatt tcaaaaatcg cagtttgacc gtctgaagcg tactggattt ctttgttttt
    17521 gccaaatgca ttagtaacga ctgctgtttg ccgcttcaac cttaaactgt ctgctaatga
    17581 tgaaaccgtt ccttctcctc catcgcctat aggtagcaga tcatattcag cctgaacaaa
    17641 aacttcttga aaacctgatt gaatggcttg tgctgcctct aaggctgtca gactttcttt
    17701 gaaagaatct ggcgcaatca gaatacgcat tattttcccc tccgttccaa gatcagttct
    17761 ttttccagct ggtattggtc tccattgaca aaaataggta atgtttcttt cgtctctttt
    17821 tttattacta tttgtttttt tgtcaaaaca aatagcaacc tgtgattttt ccaaataaaa
    17881 ggaaaagaaa ttttttccca tgctgaagga agtcttggag aaatagaaag agttccattc
    17941 gttactgtaa tacctgaaaa accaaatata actgctagcc atagtgctcc taatgaagca
    18001 gcatgaattc cgtcatctga tgaatgcgga tctggtccta aatcaatcag gcacgcttct
    18061 tggaataaat gatacgccca ttccatttct tggatacggc aagcctctat ggcgtgaatc
    18121 gctttactca acgatgagtc gtggattgtt ttgttttcat agtaatgcaa attcttagaa
    18181 acgattggtt gagaaaaata ctcaggtaat aagtataaca gcatgacaag atctgcttgc
    18241 ttgctgactt ggagttcatt tacttcgcta cgggcataat caagtaaaat agactggctg
    18301 ccaggacgtt ctttgtaagt ggtcaaatca ataactggct tactgagata cgtgtcatct
    18361 tgcggaatca gctgttcttt gttaggcact ggcaaatata acttatctaa aaaattttga
    18421 cattctttca aaaaaagcgg atcgccagct tgatatttaa cagctaaggc cacattataa
    18481 tgcgccagat agttcgtata tgcattattg tcgatatatt ctgtgtattc atctggacca
    18541 atgactctgt gtatctctaa atgatcatcg acttttacag ctctgctcag ccaaaaagct
    18601 gctgtttcat gaatgagcgt ttttcccttt gtttctaaaa acgattgatc ctctgttgct
    18661 tggacatact cttgaacggc ataagcaagg tctgcactga tatgatgttc tgcggcagct
    18721 gaagcaactt cttgtcttgt tccggttttg atattcaatg cagcaaattt gggtgtctct
    18781 tcctgaccag tgtgtgcgct ttcccatggg aaaagtgcac cttgatagtg ataatctctt
    18841 gctttttgcc gtgcgccttc taatcgatca taacggtaca gcagcaaatt ctttgctact
    18901 tcaggaaact gatataaaaa gaaaggcagg ataaaaattt ccgtatccca gaaaacatgc
    18961 cctttataac cttcgccagt tagtcccttc gcccctacac taacacttgg atcattgcgg
    19021 ggagtcataa tctgtaaatg gtaagcagcg aagtccatcg ctaactgatc gaaagaagaa
    19081 tcgccttgga tcgttatccg atgctgatgc caaaaatctg tccaataaga catcgactgc
    19141 tctaaaattt ctgtataact aacgcttttc tgggcttggg caatactttt cttaaacggg
    19201 tctgtttgct gaagagaggt agagagcacg atgatcttct ccagcgtaaa ggacgtattt
    19261 tttctgatct tttctgaaaa ggaagcggtg atccggcgat tttttgcttg gaatacacct
    19321 gctttattga atttagctgc aatcccaata tcaattttac tttctgttgt ctggtaggat
    19381 gcactgatca aatcgtcacc aaatactcgc agttcttttt caaccaactg ctgtgtacca
    19441 aaattggttt gctgtccatc gatacccgtt ttcacatcta cttggcagtc ttcggtcaac
    19501 ggcttgatga ccactttcag ggctgcctga tggagattat tttgatcagc aacacgacaa
    19561 gtaatgactt ccactttttt tccttgagca gtttcaatga gcgcatagcg tttaagctct
    19621 cccatttcta aatctagtgc ccgctcgtat gcaagaactt tcatcgtctc aagagaaaaa
    19681 tgttcaccgt cgatttttat ttcccatctg atgacatctg ggagattcac taactcggct
    19741 gattctgctc ctaaaggtcg gttatataca ccagctgcaa acattcccct tacttgattc
    19801 cgatagtctt cctcctggct tcctctgata ccaaaaaatc catttccaat ggtcatctga
    19861 ctcgcatact tattgatata ctctaaatca aactgttttt cttctacttt cataaggtaa
    19921 taacttctcc tgtttctgcc gaacgatata aattttccac cagattctgg atgacatatc
    19981 cttgccagcc atctgcaatc atctgagtct ggtcaccgag cgcccgattc acaaaatgag
    20041 ccatacttag ttcatgtggg tcattaactg gcgtggtctt ttgtaaggaa accagctttc
    20101 catctacatc ttcgaatagt tcgcctggat acagattagc acctgcctga tcgccacaaa
    20161 aagccacatt cagtttgtct tctctgatat tcaaagcaaa cgaagtattc agttggatga
    20221 tcttgccgtt ttctaattcc agtgtgccaa aaattgcatc ttctactgtg aatttgcttg
    20281 gatcccattt cccgaatttc cctgaatttt ttttcgtacc aattttttgg aagctgtacg
    20341 cccgaacgct ttttacttta ggaaatccag taatatacat cgctgtatca agcatgtgaa
    20401 tgccataatc gatcaaaggc ccgcctcctt gaagttcttt gtcgataaac gttccccagc
    20461 ctggaacacc agaccgacga agcgcattcg cttctacgta ataaatatcc ccgagaacat
    20521 caagatgatt cttgatcatt gttgcttcgt cagaaaaacg atgctggaat ccataagcca
    20581 aaatctgccc gtttttatcg gctgtctctg ccatctttct tgcttgatca gctgtcatag
    20641 ctggtggttt ctcgcacatc actgaagcat tagcttcgag ggcagccaat acatgttcga
    20701 agtgaaagcg attgggtgta cagacactca cgatatcggg agaaatctca ttcagcattt
    20761 gtgtaactga tgtatacgca tgagaaaatc ctgtacgtcg gcaaaagtct tctgctcgct
    20821 ggctgactgg atcaaccaca gcggccaatt ctaagacatc tgatcttttt tgataataag
    20881 cagcatgtac tttttctgct acttgtccag cgcctatgat tgctactttc ttcatttatt
    20941 tctctctcct ttgcttattt acttattgtt attctttctc tattctttct ctaaatcatt
    21001 taaatgacgc gctgtttcca gacagttttt caagtattgc aatgattcct gataaatttt
    21061 cacttcattt tctcctcgca aacgacattc atataccata taaccttcat actcattttg
    21121 aagtagcgtt tgaaaatggc gagtaaaatc aattgcgcca gatcctggtt ggtagcgatg
    21181 attatctgct aaatggatat gacccacata ctctttttgc gtagtcaatg cttttgaaat
    21241 atcatcttct tcaatattca tatgataaaa atcagcgatg atctgcacat gtttcaattg
    21301 attagagtca atgtaatgac gcgcatccgc taacaaattg atcatatggt cttggtatcg
    21361 gttcaaaggc tccaaataaa taaccgtacc tgtttcttcg gctactttat ctaaataacg
    21421 aagtgattcg ctgactgctt tatagtctcc ttctttgctt cggggtgaaa ccatcggcgg
    21481 taatcgataa gtgaacattc cccaagctgc tgggataacg attcctttgc ctccaacctc
    21541 gcttagtgca cgcaaaatct cagcaatttg acgtacacca ttaagacgcc gttcttcgat
    21601 aaagtcaccg atccacccat cgtagccgtt acaagctgtc actactggga tccctgtttc
    21661 tttgattgcc tgtttgactg tgtcgatatt ttctattaac aacttaccgt cgatttcgta
    21721 ttcttcaaat ccacagtctt tgataaatct aaatttttct aaaatgtctc ttgggaaaaa
    21781 tgcttgatct tgcgtaccga atttcattgt tctcctcctc ggacttgaac gcccattttg
    21841 atactttctt ctgggtgctg gtctacataa cgcatgtaag cttctgcgct ttctgcaaat
    21901 ggcacaactg gatcaatgat ttcttcacaa tccaagtaac cgttcatcaa taattcccaa
    21961 catgtgtcct cgattcgttt acggttccat cttgggtaat ctgggtttgg ttcactagat
    22021 gctcgagcaa agacgatttt tgcattattg aaatgtgctt cgcgaccaag attaaaccca
    22081 gctggaaaag gtttagcaaa ggcaacataa gaaatgatcc cgccatagcc gatccctatt
    22141 aaagaagctt gtaatgcggc agcactgcca ctcgtttcaa taactgaatc tactccacgt
    22201 ttatctgtca attcctttag acgaaaaccg acatctgctt tcataggatc tagcgtgtaa
    22261 tcggcaccgt gtttttctgc taattctcta cgctgcgcta tgggatcgat cccaacgatg
    22321 gtagtagcac ctgctttttt agctaattga atcgcaattt gcccgatagc gcctaaaccg
    22381 aagattgcta cagcatcacc tgctcgaaca tgtgcttccc ggataccacc taaagcgaat
    22441 tgagcaggat cgtaacatac tgcatttttc cattgatcca accgatcaag tttacgtagt
    22501 ttataattat caacaccgtt tgtaatcagc gtttcacgaa tgggtccata agtgcagact
    22561 aactctcctt tttgatactc tgtcaccaaa ctgcccgttt cttcgatcgt accgacaatc
    22621 atatttccta attgaaattc gccaaatacc acaccgcgtt ggcttccttc ttctcttggc
    22681 aagaataggc gccattcttc tgaaaattct tcatcgataa atggacttaa tcctctaaag
    22741 tcgacaattt ctgttcctgg ttttggagta gcgaaaatta ctcggatacg tacttcgttc
    22801 ttttcgatct cacggtcttc gtaatctttg aatccggcta ttcttggttc taatgcaact
    22861 aattttttca tcttttcttt cctctttctt ttctttttat ccttttacgc cgccttcgac
    22921 taaatcacct ttaataaatc gttcagataa cgcatacata atgatgactg gtaaagcggt
    22981 caccaatgaa gcggccatca ttcgtcccca aatgtagtct ggtgtactga ataatgtgtt
    23041 caaaccgatt ggtaaagtaa atttttcgga ggtcgacagg aaaatcgaag caaataaata
    23101 atcattccac gaaaccataa agcaataaac gaagacagaa atgattccag aaatagccaa
    23161 tggtataatg atccgaataa tgatctggat ccggctaaga ccatcgatca ttgctgcttc
    23221 ttcaatatca gtcggaatcg tgtcaaaata actttttagc atgaatatcg cagtaggaag
    23281 tgtttgaacg accatcgtga taatcagtgc tgtattggta tcatacaaac ctagaccgga
    23341 aatgatttta aataatggaa caacgatcaa aataccggaa aacatataaa cggtgtaaaa
    23401 gctagcattg attttcgagc gagctttgaa gcgtagtttt gacaaagcgt atgccccgct
    23461 gatcccaatc actactgcag ttacggaggc tatcaaggag atcactaaac tatttttgaa
    23521 atacatgatg aatggaaaaa tctcagcatt gaaaatatcc tgataatgtt caaaagtcca
    23581 ttctttcgga aaaatagttt gtgtcgttgc aattgcttct tgtgaatcct tgaatgatgt
    23641 catcagcatg atcaaaaatg gaaacaaatt gacagctaat agcaacaaaa tcgtcgcgta
    23701 aaataagatt ttcctgctct ttttattttt catttcaagt ccaccttctt tcgtgagagc
    23761 aagacgaccg ctgcaataat gatgaacaat agaatcgata tagctgctgc ttttccaaga
    23821 tcattgaacg caaaggctgt ctgatacaaa taaacgccta agatattgac tttattcgtc
    23881 agtagataga catctgtaaa catatagaac atccagatag atcgtaaaac aatcacagtt
    23941 gctagtactg gtttgattgc cggcagtgtc acgatcttga acttctgcca agcatttgca
    24001 ccgtccattt cagctgcttc ataaagcgtt ttgtcgactg tttgcaaaat agccaaaaat
    24061 gaaataaacg catacggata gtatctccag atagcaaaca aaacgaccat aaagaagcta
    24121 gcaactggct ggtcgaacca cagcggtgct tcgttgaaaa gctgcaacct atctactaac
    24181 agatcattga cgattccata actattattg aacatatatt tccaagcaaa aatcaaagaa
    24241 atagatggcg taacataact taataaaatc agtgaacgca ccgttttcct aaatctgaac
    24301 ttgcggttaa gcaaaatagc tacggctaat cctaaagctg tactgcctgc tacaactaag
    24361 aatgtgtaag caactgttaa acctaaagaa cgataaaatt cactatcact taaaatttca
    24421 agataattgc ttaatccgat aaaaacggcg tctgagttag gattcagcgg catatcgaaa
    24481 aaactgatct gaatattgga aagcattgga atcagcacta aaacagctaa taaaagcatg
    24541 cttggtgcta aaagtcccat ggctaatttt aagtcagaat gtttccttgg acgattcatt
    24601 gaaacctccc cctgataatc tgccaatagc tatctacttt tttcgaacac cattggcaga
    24661 acttcttgtt atttcgtatt tttttgtgcg tcagctaaag cttgatcgac atcttcgttt
    24721 ccgacagtta cttggttcac tgcttttccg atcacacctg agcttgtgat atctcccatt
    24781 ttcgtgaaat tcttttcccc gactagtcca aagacttgta cttcatcaaa agcactagca
    24841 atctcagatg gcagctcgcc aaaagacttg atgacttcat tttctttgta accatccagt
    24901 tcgactactt gtttattcac tggttgagca ccaccaggag acatcaagac ccacttttcc
    24961 atgtttttcg gttgagataa gaaagctgtg aatgcttcag cagcctcttt ttgtttctcg
    25021 tctagaccag aagaaatcgt caatccagat actgtgccat agacagcttg atctttttgt
    25081 gttgggatag caaaaccgat attttctgag ttccccgctt cataaacaga aggcaaaatg
    25141 taagttgaat agattgccat tggtgctgtt ccattcatga aagcatcttt gatttctgtc
    25201 acatcattag agcctggcat cgtgtattgg gataagtttt gataaaacga caatgcttct
    25261 ctcatctttt ccgtatcgat cgttacctcg ccttcatcat ccaaaacatt tgctccattt
    25321 gacaaagcaa attgagagaa cgcttgctca ctcattgttc cttcagcagt aggcatagca
    25381 attccatatt gtttattctc tttatctgtg aaatgttgag caatttttaa aatatcatcc
    25441 caattttctg gttctgaaaa accagcttct gataattttt ctttgtcata ccaaatacct
    25501 tgaacccagc cgctaattgg tgcagcaata tagctttttc catcttctga acgaacaagg
    25561 tttttggctc cgtcataata attatcttct ccagcttcct cgataacgtt ttggactgcc
    25621 gtctgatcaa tcagttcatc tttatccatc actttagcaa aatcctgact gacttccatt
    25681 acttctggta atttaccgga acgagctaac gtaacgattt ttgtattgaa cgctgattct
    25741 tctaccggca cttgcttgat tttgatgtct ggattttctt tttcaaaatc agcaaccagt
    25801 tcattgatca cacttaatcg ttcctgttcg acagaagagt gcatgaattg aatcgttgtc
    25861 tgttgtgatt cgttagagct ttgactttca ccacaagcag taagaagaaa tccagtagct
    25921 agcagacagg tagtaacaat aatttttttc atttttttcc acctcttatt ttattatttt
    25981 cagccacata tattcatacg gctggaatat caactttcgt tcttgactgc aatggataat
    26041 ttgtccagaa aacaggtcta ggtaatctcc ttttggtaaa agataacctg ctgattgatt
    26101 ggaaaaatta tggataacta agactttctc ttccttcgct tcgcgaataa aactgataaa
    26161 agaatctgct gagtccagta agtaaatcaa actattcgga tggaaggctt tttctttttt
    26221 tcgaatagat agcatctggg tcaattcacc atatactgct gttcttaatg gatctgtctg
    26281 caactctttc tcaatatctg ctaacaaaaa tttttgccga ttgatttttc gattctctcc
    26341 tgttttttct acgcctttgt aatcatttcg acttcccagc acactttgaa tataaattgc
    26401 cggcacgcct ggtaacgtta ccaaaaatga atgagcagct aaaaagcgtt tgcttctcca
    26461 ttccgtcgta tctgttggag aacttaacgc atctagatac gtagcgttta tctcatatgg
    26521 actggtcgtt ccatctgggt ttttcttgta gtttactcgg gcaccctctt tcttcagctg
    26581 ttccactaat tcaaggatcg ttttttccgg cagaatacct cgtaccggat tcaatccgat
    26641 accgtcatgg gaggctaaaa aattaaaaaa agttgttttt ttcgatgcga aatcgatggt
    26701 ttttaaccat tctttcaact tacttgtatt acccgaacga atcgcttgaa gggtcaaagg
    26761 tggtagcgaa aactgataga ccatctgtgc ttcattctcg ccattcccaa aataactgat
    26821 attatctttg tgtggcacat ttgtctcagt gatcaatacc gttcctggag ctgcttcatc
    26881 agcgattgca cggaataatt ggatgatttt atgcgtttct ggcaggtgta tacaagaagt
    26941 tcctagttct ttccataaaa atcccacagc gtccaagcga ataaatgaag cgcctttttc
    27001 taagtaaaac aaaagaatat cgatcatttt caataaaact tctggatttt taaaattcaa
    27061 gtctacttga tcttcgctaa aagtcgtcca tacgtgtttt tctttcccgt ttttgtcaat
    27121 gaacgttgta agtaacgggc tggtccttgg tctagtcacg ctgcttgtat ctgtatccag
    27181 tggcaaatca ataaaaaaat ctttgtacgt atcattcaag tttaaatatt cctgaaacca
    27241 agtactttcg gcagacatat gattgcagac aaagtcgaac attaaccgtg tttcgcttct
    27301 catttcttct atatcttccc atgtgcccaa acggtcgttg attgcctgat aatttacaac
    27361 ggagaaacca tcatcagatg tatatgggaa aaatggcaag aaatgtacaa ctctaaatgt
    27421 atctgccaaa tacttttgat aaaatttctt aaatgtacgc aaagttggtt gctgctcttc
    27481 aaaaaattga tccccataag taatcaagaa aatatcttct tcgtcccaat gcagtttgcc
    27541 tgcctggcct ttcttctgct gataaacact gactttttgt tcaatcatag aggcggtttt
    27601 ctcagacaaa tctgagtcgc gataaatcgc ttgtaaaagt tttgtataca tcggattccc
    27661 ctttcttttt tgggagcgtt cccaacgatt tgatcatatt atggaaaacg cttactgtca
    27721 atagcatttt tttaaataat tttgatatac acaaaaatac aaataagaaa gcttatgtat
    27781 caataattaa tagcttctta aaaaaatatg gtctctcatg atcatgcaag ctattcctaa
    27841 attttcaaaa tcatagacaa aaaagaatgt ctattgtact atggaattat tctaaaacgc
    27901 tcccaaaaag gaggtgcata taaaattgaa acctactatt tatgacattg cgaaaaaagc
    27961 taatgtctca aaatcaactg tctccagagt attaaataac cagccaaata tctctgataa
    28021 agcaaaagca agtgtacttc aagcaatcaa agaattgaat tatgaaccga gcaaaattgc
    28081 aagaggactg tcaggaaatg gttttgacgc gattctagca ttatctcatc gtacttctca
    28141 tacgacctat ggcaatcctt ttttttcaga tgttattcaa tccatttctg ccgtttctga
    28201 acaaaataac ttcgacttga ttctacaaac ggcaaaaaca ccagaagaag agctagaaaa
    28261 atgcgcgaaa aagatccaag aaaaaatgat ccgaggaatt attattttaa gctctacgat
    28321 ggatgaaaca cttctttttg aattggatca atacaatatt cctattgtgg taatcggaaa
    28381 agtcggaaac gactacacga acatctactc agtggacacg gataatttcc aggatagtta
    28441 cgatttggtg gaaacactga tcaagaatgg acaccgtcat atcggctgtc tccatgctcc
    28501 gacagaagtt tatgtatcca atgatcgtct caatggttac cgtcaatgct tatttgatca
    28561 ccacctagat atccgtaatg aatggattat agacggcggc tttcgcttag aagatagcat
    28621 ggcagcaatg aatgatttaa tgaagctacg agaaagacca actgcgattt tttctaccga
    28681 tgcattaaaa tcattgagta tttacaaagt ggcagaacaa tctggaaagc agatacctga
    28741 tgatctttcg atcgcaggat tcagtgatcc cttattgtcc tccctatttt cgccacagtt
    28801 gactcggata gacattccga caaatcagtt aggaatcaaa gccacagaac ttttattcca
    28861 actaatccac caccaatacc ctgaagaaaa aagaatcatt ctttcaaccg agattactct
    28921 cacagattcg atccaacaac aagaaatgta gaacagaatt ataaaagaac agaaacactg
    28981 aggataagtc agttactctg ttcttttttc aatcactcat ccctcgttac cccctcccgg
    29041 tattttagcg aatttttctt attttccatc tgttttagat agctgtggat aactataatg
    29101 tttgtgttaa actatttttt ccacagggaa gaaaaaacta tttttaacca cgttgtgtat
    29161 aagtttttat agttcgtttt tttaaacaca tttaccctta tttcaatcca tgttatttgg
    29221 ggatacttat ctctaaaaca taaatagaat aacagacatg tctactggtt ttgttttatt
    29281 cgaggaatta ttcaataata gctagcaaaa tattaaaaat aaccgctcta tcttcccatc
    29341 aatttatttt gcattcaaaa aatgatctat ctttactttt gttgattgac ttctcttgga
    29401 agatgatcgt tccgatacct taattggata cgaaccgatg ttgttaagat cctttcccac
    29461 ttttcttcta gttagacaaa ttattttaga caaaccaaaa aagaggttgt gacaataatt
    29521 tgtcacaacc tcttttttaa aatatctatt ttgtttgatt gttcattgcg tcttctgcag
    29581 ccatttgcgc ttcaatatct gaaatactgt agacattttc gcttgctaca ccgacttctt
    29641 ttggttccat gtcacgataa cgttgcatac cagtaccagc tgggatgatc ttacctataa
    29701 taacattttc ttccaaacca agaagaggat ctttcttacc gcggatcgca gcatccgtca
    29761 agacacgagt tgtttcttgg aatgatgcag cagataagaa actatttgtt tctaatgatg
    29821 cttttgtgat acctagtaag acaggtcgag aagttgcagg aacgcctcct gagactagcg
    29881 tattgtagtt ttgatcttta aagtctgcga tgtctaataa tgtacctggt aagatatctg
    29941 tatcacctgg atccattaca cgaactttac gcagcatttg acgaaccatt acttcgatat
    30001 gcttgtcgcc gatttctacc ccttgcatac ggtaaacacg ttgtacttca cgaagcaagt
    30061 agttttcaac agataataca tcgcgaactt gcaacaattg ttttggatcg attgatcctt
    30121 ctgtcaatgg tgcaccacgg tggatcatat cgccttctgc tactttcata cgtgctgtat
    30181 aaggaacagt atacgtacgt gtatctgttt ttcctttgat tgttacttct ttggtacgtg
    30241 ttgcaggatc ttctgagata tcgatgacat cccctgttac ttctgtgatc actgcttgcc
    30301 ctttcggatt acgtgcttca aagatttctt ggacacgagg aagaccttga gtgatatcgt
    30361 ctccggcaac cccacctgta tggaatgtac gcatcgtcaa ctgagtacca ggttcaccga
    30421 ttgattgagc ggcgatcgta ccaactgctt ctccgacttc aacgtcagag ccagttgcaa
    30481 ggttgcgtcc gtaacaatgt ttgcagacac catgttttgt attacatgtg aagacagaac
    30541 ggattgtcac tgtttcgaca cctgcatcaa cgatttgttt tgcgatgtct tcggaaatca
    30601 attggtttcc tgcaatgatc aattcatttg tttcaggatg acgtacttct ttacgggtgt
    30661 aacgtcccac taaacgatct tctaatgatt cgatgatttc atttccctcg cggatggctt
    30721 ggatttcaag acctcggtca gtaccgcaat cttcttcacg gatgattaca tcttgggcca
    30781 cgttaaccag acgacgagtc aagtaacctg aatcggcagt cttcaaggct gtatcggtca
    30841 tccctttacg agccccgtga gttgagataa acatttccaa gacggatagt ccttcacgga
    30901 agttcgaaat gattggcaat tccatgattc gaccattagg agcggccatc aaaccacgca
    30961 ttccggcaag ctgtgtaaag ttggaaatgt taccacgagc tccagaatca gacatcatga
    31021 agattggatt tttcgcttcc aaaccttcca tcagtttttg ttggatgctg tcttttgcag
    31081 cattccaaac agcaatgaca cgttcataac gttcgtcatc tgtgatcaga ccacgacgga
    31141 attgttttgt aatagtttca acttgtttat gggcattttc aatgatttct tgtttttcat
    31201 tcagtacact gatgtcagcg atccctactg tgataccggc atgtgtcgaa tgtttgtacc
    31261 ctaagtcttt catacggtca agcatcttag aagtttctgt tactttgaat cgtttgaaga
    31321 cttctgcgat gatgtttcct aagtttttct tcttgaacgg taatactaat tcttgctctt
    31381 tgatatgtgc aggaatatca gtaccagctt caacaaagta tttgtccggt gtttggacgg
    31441 tcaagttgaa gtctgttggt tcattcagat aagggaattc aggaggcatg atctcgttga
    31501 agatgatctt accaactgtt gtgatcatcg tacgttcttt ttgccattca gtaaatggtt
    31561 tttcacctaa tgagttaggg ttcacaccga tacgtgaatg taaatgaaca tatccgttgc
    31621 gccatgcgat aacggcttcg ttttgatctc tgaagaccat tccttcgcct tcgcgtcctt
    31681 cttcttccat tgtcaagtag tagttaccta gaaccatatc ttgagaagga gtaacaactg
    31741 gtttaccatc tttagggttc aagatgtttt gagcagctaa catcaacata cgtgcttctg
    31801 cttgtgcttc ttcattcaat ggaacgtgga cagccatttg gtctccatcg aaatcggcat
    31861 tataagcttc acatacaagt ggatgcaaac ggattgcgcg tccttgtact aaaactggtt
    31921 cgaatgcttg gataccaagt cggtgaagcg taggtgcccg gttcagcaat actgggtgtt
    31981 ccttgattac gtcttctaaa acatcccata cttcgtcttc ttgacgttcg attttacgtt
    32041 tcgcattttt gatgtttgaa gctagttcgc gttgaacaag ctcacgcatc acgaatggtt
    32101 tgaataattc aatggccatt tcttttggaa gtccacattg gtacatcttc aagaaaggtc
    32161 caacaacgat aacggaacga cctgagtagt cgacacgttt acctagcaag ttttgacgga
    32221 aacgaccttg tttccctttc aacatatgag aaagagattt caatgggcgg ttacctggtc
    32281 cagtaactgg acgtccgcga cgaccgttat cgatcaatgc atctactgct tcttgcagca
    32341 tacgtttttc attttgaacg atgattccag gtgcattcaa gtctaacaaa cgtttcaaac
    32401 ggttattacg gttgattaca cgacggtaaa gatcattcaa gtcacttgtt gcaaaacgtc
    32461 caccttctaa ttgaaccatt gggcgtaaat ctggtggaat aacagggata acgtccataa
    32521 ccatccagct tggttgattt cctgaagcgc ggaatgcttc taaaatatcc aaacggcgga
    32581 ttgcacgtgt acgtttttgt ccttgtgcag atttcaattc ttctttcaat tcggctgctt
    32641 ctgcatctag atcaacatta tcaagtaatt gtttaatcgc ttctgcaccc atagcagctt
    32701 ggaattcttg tccatattgt tcacgttttt cgcgatattc gcgttctgtc aacaattgtt
    32761 ttttctctaa attcgtattt cctggttcga tgactacata tgaagcaaaa taaatgattt
    32821 cttccaaagc gcgtgggctc atgtctaata caagacccat acgtgaagga atccctttaa
    32881 agtaccagat atgtgaaaca ggagctgcta attcaatatg tcccatgcgt tcacggcgaa
    32941 ctttcgaacg agtaacttcc actccgcatc gatcacaaac gattccttta taacggatac
    33001 gtttgtattt tccgcaggca cattcccagt cttttgtagg accaaaaatc cgctcacaga
    33061 acaacccttc gcgttcaggt tttaaggtac gatagttaat cgtctcaggt ttttttactt
    33121 caccgtaaga ccagcttctg attttttcgg gagaagccaa acctatttgc atactttcga
    33181 atttatttac atcgatcaaa aggggttccc tccatttcat ttaatggcaa accgcaagac
    33241 ggctgatctt cgaccgcctt gcagctcata gaccggatta gtctaaatta tcttctgcgg
    33301 tttcaaagtt ttggatgaca tcgtcttttt catcttccac ttgttcagca gctttctttt
    33361 ctaattcttt ggcagtttgc tgttcggcaa attttgtcaa agcatcaaca gtgatcaaat
    33421 cgtcgtcttc atcgtccatg tcgcgaagtt cgatctctgt ttcttcgata tccaagacac
    33481 gcatgtccag acctagtgat tgcaattctt tcaccaatac gcggaatgat tccggtacac
    33541 ctggttttgg aattggttca cccttgacga ttgcttcgta tgttttcaca cgtccgacta
    33601 cgtcatctga tttgtatgtc aagatttctt gtaaagtata agccgcaccg taagcttcca
    33661 gtgcccatac ttccatttca ccaaaacgtt gtccaccaaa ttgagcttta cctcccaatg
    33721 gttgttgtgt aaccaatgag taaggtccga ttgaacgtgc atgcaatttg tcatcgacca
    33781 tgtgagcaag tttgatcatg tacatcacac cgacagacac acgtccatca aatggttcac
    33841 ctgtacgtcc gtcgtacaga acagttttcg catcgctggc cataccggct tcacgaactg
    33901 tttcccatac gtcttcatca cttgcaccat caaatactgg cgttgctacg tggataccta
    33961 actgacgtgc agccataccc aagtgtaatt ccaatacttg cccgatgttc atacgtgaag
    34021 gcacccctaa aggattcagc atgatatcga ttggtgttcc atcaggtagg aatggcatat
    34081 cttcttccgg cataatacgg gatacaaccc ctttatttcc gtgacgaccg gccattttat
    34141 ccccttcatg gattttacgt ttttgaacga tataaacacg aactaacatg tttacgcctg
    34201 gtgacaattc atctccagct tcacgagtaa agattttcac atcgtgaacg ataccgccgc
    34261 cgccatgtgg tacacgtaat gatgtatcgc ggacttcacg agctttttcg ccaaagatcg
    34321 catgcaatag acgttcttct gcagataatt ctgttacccc ttttggcgtg actttcccta
    34381 caagcaaatc gccatcttgg acttctgcac caatacggat gatacccatt tcgtctaaat
    34441 cttttaatgc gtcttccccg acattaggga tttcgcgtgt gatttcttca ggtcctaatt
    34501 ttgtatcgcg ggcttctgat tcatattctt caatatggat agaagtgtat acgtcatctt
    34561 ttaccaaacg acggctcatg atgatcgcat cttcatagtt gtacccttcc caagtcatga
    34621 aaccaactaa aacgttttgg cctagagcca tttcgccttg ttccatagat ggtccgtctg
    34681 ccagagtatc ccctttttcg actttctcac ccaaatgaac gattggacgt tggttgtagc
    34741 ttgttccgga gtttgaacga cggaattttg ttacatcgta tttatctaat gcaccgttgt
    34801 cgcggcgtac gcggatttca gaagcatcaa cgtattcaac gacaccgtca tgtttacata
    34861 acaaagcagc tcctgagtca tgcgctgatt tgtattccat accagtacca acccatggtg
    34921 cttgaggatt gatcaacggc actgcttgac gctgcatgtt ggcacccatc aaggcacggt
    34981 tggagtcatc gttttccaag aaaggaatac atgctgtcgc aacggcaact acttgtttag
    35041 gtgatacgtc catgtagtct actttatcaa tagatacttc taagttttca ctttgagcac
    35101 gagccataac aacgtcttga gcaaatgttc catcttcatt caatgggctg tttgcttggg
    35161 ctacgatgta atggtcttcg atatcagctg tcaagtagtc gatttggtca gtaacacgac
    35221 ctgtttcacg gtctacacga cgataaggtg tttcgataaa tccgaattta tttactttcg
    35281 cataactgga caaactattg atcaacccga tgtttgggcc ttcaggagtt tcgatcggac
    35341 acatacggcc atagtgggag tagtgaacgt cacgaacttc ataaccggcg cggtcacgag
    35401 tcaatccgcc aggtcctaag gctgaaagac gacgtttgtg tgtcaattcg ccaagagggt
    35461 ttgtttgatc catgaactgt gacaactgag aagaaccaaa gaattctttg atacttgcca
    35521 caactggacg gatattgatc aattgttgag gtgtcaatgt ttcagtatct tggatcgaca
    35581 ttctttcacg aaccacacgt tccatacgtg ctagcccgat acggaattgg ttttgtaaaa
    35641 gttcaccgac tgaacggata cgacgatttc ctaagtggtc gatatcatcg acattgccga
    35701 taccttccat caagttcaag aaatagctca ttgaagcaat gatgtcggct ggacgaaccg
    35761 tttttacagc tgcttctgga tagccgtttc cgatcacgtt tacttcacgt tcagggtctc
    35821 ttggtgaaaa tactttgatg acttggacag tcattggatc tgtgaccaca ccgtcttctg
    35881 atggatagta agtcactgaa tttaggccat tatcaatgaa aggagctagt gtttccatga
    35941 cttggtgagt cagaactgtt cctttttcaa cgatgatctc gcctgtttct ggatcaacta
    36001 atgtttcagc taatgtcaga ttcaatagac gtgttttcaa atctaatttt ttgtttactt
    36061 tgtaacgacc aacgtttgct aagtcgtaac gttttggatc gaagaaacga gcattcagca
    36121 agttacgtga gctgtctgct gtttttggtt cgcctggacg caaacgttcg taaacgtctt
    36181 tcaaaccttc ttctgttctt gagtcgcttg cgtttttatg caagtctttt tcgactgtat
    36241 tgcgtaatgt ttcgctgtcg ccgaagattt caaagatcgt atcatcggaa ccaaatccta
    36301 acgcacgaac taatacggtt aaaggaatct tacgtgtacg gtcgatacgt acataagaga
    36361 tgtcttttgc atcagtttcc atttctagcc aagctccacg gttagggata acagtagaac
    36421 cgaatccttc ttttccgttt ttgtcgactt ttccatggaa atagacacct ggtgaacgga
    36481 ctaactgaga aacgattact cgctctgccc cgttgatgat gaatgttcct tgttctgtca
    36541 ttaatgggaa atcaccgaag aatacttctt gtgctttgat ttcgccagtc tcgcggtttg
    36601 tcaaacgcaa tgttacatgt aatggtgcag agtagtttgc atcatgcgca cgagcttctg
    36661 ctactgtata ctttggttct ttcaattcat aatctacaaa ttccaaagat agattgccat
    36721 tgaagtcatc gattggcaaa atgtcttcaa acatttctct aagtccctca tctaagaacc
    36781 attggtaaga gtctgtttga atttcgatca aatttggtaa ttccaacact tcactgattc
    36841 ttgcgaaact tctacgttcg cgatgttttc cgtattttac tacgtgtcca gccaagctct
    36901 tcacccctct aaaatgtcat tcaaaaagac tacccgatca attgttacaa taatatttca
    36961 aatattattt ttaggtaaca acagacattt tttgcggctt ttaggcaaaa aaaaaccaaa
    37021 aatagaacat tctcttgaaa ccttacttcg tgagcttcta cttttgtttc cttatattaa
    37081 agccgaaacg gacaatattt cgtttctgcc ttttcttcat cagatagttt ttgcaacttc
    37141 atatactaca cttattttct ataaaagtca acacttcctg gcgtttcttc tgctttttcg
    37201 caacattttc ttaatgtttt caattttatg ttttcaatta tctgacaatt cttaattgac
    37261 agaccttttt atctcggtta tactttagaa aattcatttt gtggaggaac ttctatgcga
    37321 accaacatca accaaatcgc accaacacaa ctgaataact tttactttag ctactttaga
    37381 taggtttgta ttcatcatga tggatacaaa cgttaaacga cagtttgtat ctatgatggc
    37441 taaagaactt tgtattgcag caaaaagcca cgtagacaaa atgctaagtg gctgagcagt
    37501 gaaaatacaa agtaaaagta tcaagcattc tactttttca atttattttc cgacttttca
    37561 atccatttgg acattttgtc tgaatggatt ttttgtttcc caaaattaag gaggaaaaag
    37621 aaatgaaaaa aaatataatc atcggattag gcgctgccct gctactagct ttaggaggtt
    37681 gcgcacaaaa agacaattcc gcagaggcat cagatggaca aaaaacaatt ggcgtattgc
    37741 aacttgtaca acataactcg ttagatgctg cctataaagg attcaaagaa ggattagcag
    37801 aagaagggta taaagaaggc gataatctaa caatcgatta tcaaaatgcc caaaacaatc
    37861 aagacaacct gaaaagtatg agtgagaaac tcgtcaaaga agatcctgat ttgctacttg
    37921 gaatcgccac tccagcagca caatctttat taaatgaaac aaccactatc ccgatgactg
    37981 tcacagcggt cacagacttg gaacaagccc atttagtcac atccaatgaa aaacctggcg
    38041 gcaatgtgac tggcacgaca gatatggtgc caatcgaaaa acaaatcggc ttactgttaa
    38101 atatcgtgcc agacgccaaa acaatcggca tcatgtataa caacggtgaa gcaaactcta
    38161 agatccaagc tgatttggca gaaaaagaat tgaaaaaagc gggcgtcaaa gtcaaagtac
    38221 tgacagcaaa ttccacaaat gatgtccaac aagtgacgaa aagtctagct aaagatgtag
    38281 atggaatcta tattcctact gataacacgt tcgcaagtgc ggcaagtgtt gtcggtgaag
    38341 tcgcaaaaga aacaaaaaca cctgtcgttg ctggcgcagt tgatcaagtc aaagacggcg
    38401 gtctagcaac agtaggaatc gactacgaaa gcttgggcaa acaaacaggg atcatggcag
    38461 caaaaatctt agacggcaag aatccaagtg aacttcctgt cgaaacagcg aatgatttgg
    38521 aactgtatgt caataaagac atggccaaag cattaggcat caatccagac agcatcaaag
    38581 aagctgaata aaacacagga ggattctcat gacaaatttg ctctctcttt tcttatcatc
    38641 tgcatcgcaa ggggtcttat gggccctact tgcgattggt gtatacgtca ctttccgtat
    38701 cttggacatt gccgacttga ctgctgaagg aagctttcct ttaggtgcag ggatcgcggc
    38761 agtaagtatt acaaatggct atcatccatt gacagcttgt cttctaggct ttttaggggg
    38821 tgcggcagca gggcttgttt ccggactgct tcacacaaaa ttgaaaattc ccgcccttct
    38881 tgcagggatc atcaccatga ctggtttata ttctgtcaca tcccgtgtga tgggtgcgcc
    38941 gaacatctct ttattgggtc agccaacgat ctttcattgg gtcgagtcat tcggcataac
    39001 aaaagacaac gctgtactga tcgtcggtct gctttttgct ttgatcgttg tatgtttact
    39061 tgttttgttc ttacgtacag aaacaggact tgctattcgt gcaactggcg ataatctggc
    39121 aatgagcgaa gcaaacggga tcaacacaga taatatgaag atcatcggtt atatgatttc
    39181 taatggatgc attgctcttt ctggctcctt acttgcgcaa aacaacggct ttgctgattt
    39241 aaattctggt attggaacga tcgtaattgg acttgcttct attatcatcg cagaagtttt
    39301 gttacgtaac cagccactat ttctccgtct cttgactgtc gttttaggtg cggtcattta
    39361 tcgttttatt ttagcgttag tcttcgaact gaatgttgaa ccaagtgatt cgaaacttgc
    39421 ttctgctttc gtgctagtga tctgtctttc tttcccgcag atccagcaaa aattccattt
    39481 acctcaaaaa aagaaaggcg gtagtgtcaa atgaatgacg tcttaacatt ggaaaatctc
    39541 catcatacct ttgaaaaagg aacgatcaac gaaaatcatg tcctgaaagg cattaatctt
    39601 tccttagatc ccggcgactt cgtgacgatc atcggtggaa atggggctgg taaatctact
    39661 ttgctgaaca gcatcgccgg tacattttct gtcgatcagg gtcaaatttt attgaatgga
    39721 aaaaatatca cgaaaaaatc tgttgtcgaa cgttcgaaat ccatcagtcg cgtattccaa
    39781 gatcctaaac tagggacagc cgttcgttta acggtagaag aaaatcttgc tcttgctatg
    39841 aaacgaggaa aaaaacgcgg cttcttccgt ggtgtcaaac cacaagaccg aacatttttc
    39901 aaagaccagc tggttcggct aaatttgggg ttggaaaatc gtttatcctc agaaatcggt
    39961 ttgctgtctg gaggacaacg tcaagctatt acgttattga tggccacatt acagcgtcca
    40021 gaactgatct tattagatga acacactgca gcgcttgacc ctgctacttc caaaacggtg
    40081 atggaattga cagaaaagct gatcactgag cagaatctca ctgcttttat ggtcactcat
    40141 gacatggaag acgcgatccg ctatgggagt cgactgatca tgctgcatca aggaaggatc
    40201 gtcgtagata tccagcaaga ggaaaaacag cagctcacag tgccccaatt attagcgttg
    40261 ttcaaacaaa acagcggcac ggcattaaaa gacgatcaag tacttttatc ttcataatca
    40321 gaaagaggat gggacaaaaa atgaacgccc accctcttct tttttctctt tccttctttc
    40381 gtttttgctt tatgtatcaa ccgttgtttc ttcgaaaact attttgttct ctccttcttc
    40441 ctgatagaat ggatgggaaa ggagcttgat gccatgacaa cgaaagacaa ggtacttcaa
    40501 ttattgaaaa agacaccaga ttttctttcc ggagaaaagt tggcacagca actggaagtt
    40561 tcccggacaa gtatttggaa agccatcaaa gaattggaaa aggcaggcta tcgttttgag
    40621 cacgggccta cagggtatcg ttacctgccc tctgacgtac tagatgcaaa agaaatccag
    40681 caagagctac ggtcgtttat tcctgatttg aaagtacaaa cgatgttgac ttctgaatct
    40741 acgatgaaag atgccaaact ggcagcagct caaggagatt ctgtttcgaa gttatttgtt
    40801 gctgatacac aagaacaagc tcgtggacgt tttggacggc cttttttcgc tgaacctgga
    40861 cgaggaatct acatgagtct cttgctccat ccagacaaac ggtttgatga attgcctcaa
    40921 tatacgatca tcgcagccag tgcctgtgtg aaagccattg aatctgtaac cggtcgttct
    40981 gcttcgatca aatgggtcaa tgatatctac atggatggaa aaaaagtctg tgggatttta
    41041 tctgaagcag tcagtgatat ggaaagcggc aggatcagtc atatcatcat tgggatcggg
    41101 cttaattttt ctgttcctca aaatcatttt ccagctgatc tccaagaaaa agccatctct
    41161 gtctttcccg acggacaagc tagtgtcaca aggaatcagc taatcagcaa aatctggcaa
    41221 gaattgttca cttatctttc tcacccagaa aaagcgttgg acttctataa agaaaaatca
    41281 ttcgtattgg gaaaaaccgt gtcgttcaat caaaaaggaa cgacttatgt cggtcttgca
    41341 aaagaaatcg gtgatcatgg cgaactgatc gtccaaacaa atgaacagaa gatgagtttg
    41401 tcttctggcg aaatcagtct cacatctatc cgtacgcaat aattatcaga tttcacaaaa
    41461 ggacacctga tctcatacag tgtccttttt tctgaattct cttcaaaaga ctctccttat
    41521 tctatcagct gacagtttct ctatcctcta gaaaaaggta ttctaaaaat atctataaag
    41581 catggtacac tttctttaaa tgaatcaatg caaaaaacaa gagagtcatc agtttacacg
    41641 atcaaaagag atagcaggcc gcttcctttt tcaatcttat agatagacca tgggtaacga
    41701 taaaaacaaa aaagaacttg cgacttattt tgccgtaagt tcttttttag ctagtgcctt
    41761 aatctcttaa tcccaacaaa cgtctcgcat agatcacctg accagagtgc atagcagcat
    41821 catcaataat agaaacgaga cgcattcctc gtgtgactgg tggtgtccag ttctcgtcaa
    41881 ttacttcatc tagctcggaa ggttccagcc ttcttaagta agaaatagca aaatcagcag
    41941 ctcctcgcaa gtatcctagc agatcttcta tttgattagc ttggatctgc tttgcttgtt
    42001 ccagactatg cttccaatct tgttcgttat ctgctatcgt catcggaaac cggctttccc
    42061 agtcttgact atgccaaatt ggagaagttc ctgctaaatc agcaatctgg aaatctaatt
    42121 ctcgagcggt atgccaagcc agccaagtca ttgatttgat ctgcggggct ctatcttcca
    42181 ctggaaaacg attggcctcc tcaatagaga cgccttcaaa caaacgttca aaccgatcgt
    42241 atgcccgatc cacactctcg catagcatat ctaaataatc catttatttt cctccttttt
    42301 atttatccta cttacttttt cctaattcta ggatacctac ttatatgtta ctacacaccc
    42361 ttatttttta cactttaaca gtcaccatca tcaatagttt ggagattgaa agaaaaagat
    42421 agtgtcgaac tatcaattga acaagaagca acaatatgaa cgtttcatta atattcttta
    42481 aataaacaat gatttttcaa acgaacagtt taagactagg agaaaaagag tataatagga
    42541 aaatgtttga aaggaaagga aggggaaaaa tgaaaaaaag ggcaagttta ctagtcctag
    42601 ttgctttgtt gttcaatatc ttttcgtatg gtgtggccgt ttcagcggaa acatacattc
    42661 ctaaagaccc tgggaatatt tataatgatc taactggtaa ctatggcttg ttaggaattg
    42721 ctagtcaatt ccacgtattt gctaaaaata agacaactgt gaatgcacat acagacggaa
    42781 atatagcaac acatgaactt gatgcaaaaa ataattttgg tacagagctt catgaaggtt
    42841 tattgaacca agaaataaat tatatacaaa aaactgattc tttcgctaat tcttcaggca
    42901 tacctacagg tagcacaaga atgaataaat ttgtcgttgg cagtaacgtg gatgtagcac
    42961 ttcaagatgg tcatcctaca gtcaatggat ccaggcttga tcacttaact ccagatgatt
    43021 tttataaaga tcgtgctggg catgaatata ttgatttcga gcaggaattt gctaaattga
    43081 atgtagcagc aaatgatctt gcaacgatca ctccagctaa aacatatact gctgcagatt
    43141 ttccagacat gaacaatcgg acaatcgatt taactgattt atcagatacc gggtttttat
    43201 tggtaaatat cgatgcagaa gtgttaacga tgaatacgcc acttcaaatc attaatccaa
    43261 atgatcaagt agttgtattt aatgtcatca acagttcttc tgcattgaac gtacagtcac
    43321 caatcaaata caatgatcgt tcaaatcatg aaacagaaga tttttcggat gcgaatatct
    43381 cttggaattt tggaaatgag atgactgatt tgacaatcag tgctcctttt caaggaacaa
    43441 tccttgcgcc taatgctact atccgcatta ctcaaaacca agacggaacg attataggaa
    43501 caaatgtgat tttagatgca gctaccaatc gttgggatcc taacgaaata tttatcagcg
    43561 acaatggtac agatacgaca gacacaagcg acagtacaga tacatctacc acaagtgata
    43621 gctcagattc atca
//
LOCUS       DAMEET010000009        42372 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.009, whole genome shotgun sequence.
ACCESSION   DAMEET010000009 DAMEET010000000
VERSION     DAMEET010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 42372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.009"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            5..1030
                     /locus_tag="PB780_000453"
     CDS             5..1030
                     /locus_tag="PB780_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDE0732528.1"
                     /translation="MQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGAGKS
                     TLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVLENI
                     QLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALANDPEI
                     LLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMELGKV
                     VEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSERNA
                     NEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQGVG
                     IEVIHRG"
     gene            1023..1709
                     /locus_tag="PB780_000454"
     CDS             1023..1709
                     /locus_tag="PB780_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDE0732529.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            1733..2581
                     /locus_tag="PB780_000455"
     CDS             1733..2581
                     /locus_tag="PB780_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDE0732530.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            2778..3551
                     /gene="sufC"
                     /locus_tag="PB780_000456"
     CDS             2778..3551
                     /gene="sufC"
                     /locus_tag="PB780_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDE0732531.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            3564..4850
                     /gene="sufD"
                     /locus_tag="PB780_000457"
     CDS             3564..4850
                     /gene="sufD"
                     /locus_tag="PB780_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDE0732532.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            4847..6082
                     /locus_tag="PB780_000458"
     CDS             4847..6082
                     /locus_tag="PB780_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDE0732533.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            6069..6539
                     /locus_tag="PB780_000459"
     CDS             6069..6539
                     /locus_tag="PB780_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HDE0732534.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            6544..7935
                     /gene="sufB"
                     /locus_tag="PB780_000460"
     CDS             6544..7935
                     /gene="sufB"
                     /locus_tag="PB780_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDE0732535.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(8031..9173)
                     /locus_tag="PB780_000461"
     CDS             complement(8031..9173)
                     /locus_tag="PB780_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDE0732536.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWIAHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNDLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGNVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(9212..9946)
                     /locus_tag="PB780_000462"
     CDS             complement(9212..9946)
                     /locus_tag="PB780_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDE0732537.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLRNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            10178..10453
                     /locus_tag="PB780_000463"
     CDS             10178..10453
                     /locus_tag="PB780_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDE0732538.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMEQYKG"
     gene            10551..12548
                     /locus_tag="PB780_000464"
     CDS             10551..12548
                     /locus_tag="PB780_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732539.1"
                     /translation="METPMINIPINVDKERLLEKPVIEDEKKRIRKRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNKSEEFLETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKALSINLVHNSAFAIQKSIRYSENLEKFKIVFLLKEAITDYSKMVAVYEYLKKI
                     IPGCDDNVNASNRMFFGGYKSDAVIMINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMDKLLKSSKNL
                     RCLFHDDHNPSASIFTSKTGHSIFYCHSSNCSISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMEDKLFRTIRNYLEEMRHVYDILLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGVVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(12624..12959)
                     /locus_tag="PB780_000465"
     CDS             complement(12624..12959)
                     /locus_tag="PB780_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDE0732540.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            13087..13302
                     /locus_tag="PB780_000466"
     CDS             13087..13302
                     /locus_tag="PB780_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDE0732541.1"
                     /translation="MKKVSNLSRYRKEKGFSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            13339..13701
                     /locus_tag="PB780_000467"
     CDS             13339..13701
                     /locus_tag="PB780_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDE0732542.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            13747..14073
                     /locus_tag="PB780_000468"
     CDS             13747..14073
                     /locus_tag="PB780_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732543.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYSLNRTCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYT
                     EVLMLN"
     gene            14560..14697
                     /locus_tag="PB780_000469"
     CDS             14560..14697
                     /locus_tag="PB780_000469"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732544.1"
                     /translation="MAHNERFKQEIIQELEKGVQHQKLISKTAQKFDISLAKEVKLHE
                     V"
     gene            14762..15544
                     /locus_tag="PB780_000470"
     CDS             14762..15544
                     /locus_tag="PB780_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732545.1"
                     /translation="MKNSSNSAEVNVSTSTSEAVQCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDGVKYSEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKKVSGENNETEAVTEQTMDERIDSSESNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKNVPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(16038..17327)
                     /gene="dcm"
                     /locus_tag="PB780_000471"
     CDS             complement(16038..17327)
                     /gene="dcm"
                     /locus_tag="PB780_000471"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HDE0732546.1"
                     /translation="MFANMSETLNVLELFAGVGGFRVGLEHSNADLFKTKWANQWEPS
                     RKSQDAFEVYDYRFPNSENINRNIEEISNEEFEQMDADIIVGGFPCQDYSVARSKKHE
                     MGIEGKKGVLFWQIIRATNHIKPKYLILENVDRLLKSPSKQRGRDFAIMLASFNDLGY
                     SVEWRVINAADYGRRQRRRRVFFFVYKNDTGFANKIDARFEQKILGEEEHRYDEYIFS
                     EGLFARQFPIIKTPYKGRQASYKLSNDIVEISDNFTGKVYNTGVMRHGRYFTIDTEPY
                     DKEKPTPLREIIQPEDEVAEKYYLTDESKIKKFEYLRGPKKIERVTAEGHTYTFSEGG
                     MSPYDDLDMPGRTMLTSEGSVNRSTHLLKIDGRYRLLTPIEAERLQDFPDDWTKYKKL
                     SSGEIVEVSDRMRLFFMGNALVTKIVERIGDELQQLL"
     gene            17538..19016
                     /locus_tag="PB780_000472"
     CDS             17538..19016
                     /locus_tag="PB780_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutH"
                     /protein_id="HDE0732547.1"
                     /translation="MSEYLTKEQVHNRAKEAVGKSMIELNNGIPLLNSKNKNSIGDIF
                     ESWFGKVKDSESRPDLEEAGVELKATPIKKLKNGQYSAKERLVLNIINYNEVAEETFE
                     ESHFLYKNNIMEIAFYEYLKDTPKDQWTIREAILYEMYKNPVDFEIIRQDWELIKKYI
                     LDGKAHELSESLTNYLAPCTKGTNAVKSLRTQPYSEQKAKQRAYSLKSGYMTSILRKY
                     VLGDEKIDSIVKNTFQLEENNLEDLVLEHFIPYVGQTVQSLCEKFTIKTSYQANYRIV
                     SSILGLSGTVTKGEPFPRVEEFEKASILVKTIYFDKSGNNKESMSFPSFNFIDLSKQV
                     WEKEDGEPSSDWHNFLLDTRFLFVVFKEEDEGVVFKGAKFFSMPEEDIEGPVRKVWED
                     TVSKLREGVELEAIPDKSTKDGWKIKNNFIKKTDKMICHVRPHASQRDYSVDGRYADK
                     LPVPAKWINKPDNDNYSDQWMTKQCFWINNDYIKEQVQDLIS"
     gene            complement(19030..19461)
                     /locus_tag="PB780_000473"
     CDS             complement(19030..19461)
                     /locus_tag="PB780_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HDE0732548.1"
                     /translation="MKHRQLSTTPEVSKRMANVRLKGGKAESLLAKELWHQGVRYRKN
                     YKKLPGSPDIAVTKYKLAIFVDGEFWHGHDWDNRKQKLKRNRDYWIEKIEENMRRDER
                     NNQSLIKLGWVPIHFWEKDILKNLNYCTSVILDYIAQKKEE"
     gene            complement(21033..21698)
                     /locus_tag="PB780_000474"
     CDS             complement(21033..21698)
                     /locus_tag="PB780_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDE0732549.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            21878..23860
                     /locus_tag="PB780_000475"
     CDS             21878..23860
                     /locus_tag="PB780_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HDE0732550.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            23864..24502
                     /locus_tag="PB780_000476"
     CDS             23864..24502
                     /locus_tag="PB780_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDE0732551.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            24530..24958
                     /locus_tag="PB780_000477"
     CDS             24530..24958
                     /locus_tag="PB780_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732552.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            25415..26326
                     /locus_tag="PB780_000478"
     CDS             25415..26326
                     /locus_tag="PB780_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDE0732553.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            26543..26980
                     /locus_tag="PB780_000479"
     CDS             26543..26980
                     /locus_tag="PB780_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732554.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            27075..27959
                     /locus_tag="PB780_000480"
     CDS             27075..27959
                     /locus_tag="PB780_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDE0732555.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            28183..31350
                     /locus_tag="PB780_000481"
     CDS             28183..31350
                     /locus_tag="PB780_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732556.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            31370..31915
                     /locus_tag="PB780_000482"
     CDS             31370..31915
                     /locus_tag="PB780_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HDE0732557.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            31943..32398
                     /locus_tag="PB780_000483"
     CDS             31943..32398
                     /locus_tag="PB780_000483"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HDE0732558.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            32395..33153
                     /locus_tag="PB780_000484"
     CDS             32395..33153
                     /locus_tag="PB780_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDE0732559.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(33239..33979)
                     /locus_tag="PB780_000485"
     CDS             complement(33239..33979)
                     /locus_tag="PB780_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDE0732560.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            34040..35293
                     /locus_tag="PB780_000486"
     CDS             34040..35293
                     /locus_tag="PB780_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDE0732561.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            35615..35842
                     /locus_tag="PB780_000487"
     CDS             35615..35842
                     /locus_tag="PB780_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDE0732562.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            35965..36291
                     /locus_tag="PB780_000488"
     CDS             35965..36291
                     /locus_tag="PB780_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDE0732563.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            36359..37072
                     /locus_tag="PB780_000489"
     CDS             36359..37072
                     /locus_tag="PB780_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HDE0732564.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            37382..38233
                     /locus_tag="PB780_000490"
     CDS             37382..38233
                     /locus_tag="PB780_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDE0732565.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            38354..40219
                     /locus_tag="PB780_000491"
     CDS             38354..40219
                     /locus_tag="PB780_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDE0732566.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            40267..41676
                     /locus_tag="PB780_000492"
     CDS             40267..41676
                     /locus_tag="PB780_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDE0732567.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            41784..>42372
                     /locus_tag="PB780_000493"
     CDS             41784..>42372
                     /locus_tag="PB780_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDE0732568.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQ"
BASE COUNT        14229 a         7043 c         8579 g        12521 t
ORIGIN      
        1 attaatccaa ctaacgaatg tcaaaaagac attcaatggg aaaaacggaa acatccaagc
       61 cgtcaatgac gtttcactaa acgttgaaaa aggggatatt tatggaatcg tcggctattc
      121 tggcgcagga aaaagtacat tagtccgtct cctgaatgga cttgagcttc ccacgagcgg
      181 cgaagtgatc gtaaacgatc aagatattac aaaattaaaa aacaaagaat tacgtacatt
      241 ccgaaagaaa atcggtatga tcttccagca tttcaacctg ttatggtcaa gaactgtact
      301 tgaaaatatc cagctgccgc tggaattagc tggcgtacca aagagcaaac gtaaagaacg
      361 ggcagaagaa ctgttgcgtt tagtcgggtt agaaggacgc ggacaagcat atccttcaca
      421 actttccggc ggacagaaac aacgcgtcgg tatcgcacga gcattggcaa atgatcctga
      481 gatcctgctt tgtgatgagg ctactagcgc attggacccg caaacaacag aagaagtatt
      541 agacttattg ttagcgatca ataagaaatt gaatttgacc atcgtcttga tcacacatga
      601 aatgaatgtg atccgcaaaa tctgcaacaa agtcgctgtg atggagttgg ggaaagtcgt
      661 agaagaagga gacgtattga ctgtcttcag acaaccgaaa aaagaagtaa ccaaacgctt
      721 tgtacaacaa gacatcgaac cagaagctga ttcgacagaa ctattagctg aactcatcaa
      781 agaaaatcca agcggccgtt tggcaacttt gacctttagc gaaagaaatg ccaacgagcc
      841 agtcatttcc caagcgattc gacgctataa catcgatgtc aacgttgtgt atggaaagat
      901 caaacaagtg aaagaaggtt cctttggttc attgactgtc ttgatgactg gtgaaaccaa
      961 agaattagat caagcagaag tgttcatcaa agaacaagga gtaggaattg aggtgatcca
     1021 tcgtggataa aagcttttca gaaacatatc tagattttaa acaaatcaac ccggaaacga
     1081 tgaatcaggc tatccttgat acactatata tgacattaat ctcaatggtc ttagtgacga
     1141 tcatcggatt agttttggga ttgatccttt actctattgg acgcaaaaac aaaccgtatg
     1201 ctcgaatcat ttatggaatc gtatcgatca tcagtaatat tttccggtca acgccattta
     1261 tgatcctgat ggttttgatc attccattta ctaaagcaat cgtaggcact atgctaggtg
     1321 cgcaagcagc cattccagct cttgttttat ccgcagcacc attttatgcc cgtctagtag
     1381 agattgcttt tcgtgaagtc agccctggtg tactggaagc agcagatgca atgggagcaa
     1441 gttactggga aatcatctgg aaggtcttga tacctgaaag tattcccgca ttgatttctg
     1501 gattaacagt tacaacgatc tcgatgatcg gatttactgc gatggccggc gcgatcggcg
     1561 caggtggact tggaggtctt gcttggcagg aaggctacca gcgtggtaat ctaacagtga
     1621 cattcgttgc gactttaatt attttactaa tcgtatttat cgttcaagga atcggtgatt
     1681 tcttaactaa gaaaacagac aaaagataat aggatatagg gggatgtagg atatgaaaaa
     1741 gaaaattttt ggatttgcag cagcgctttt gctgactgta ggattagcag catgcggtaa
     1801 caacagcgac agcaaagatt cagcaaataa agcagacgac acgacattga aagtcggcgc
     1861 ttcaccgaca ccgcacgcag agattttgga acatgtgaaa ccattattga aagatgaagg
     1921 aatcgatctt gaaatcgtca aattcgatga ctatgtatta ccgaaccaat ctttggaaga
     1981 aggcgacatt gatgccaatt acttccagca tattccttat ttgaacaaag aaatcaaaga
     2041 aaaaggatat gactttgtca atgccggtgc tgtccacatc gaaccaatgg gcttgtactc
     2101 taaaaaagtc aaagatgttt ccgaattaaa agatggtgca acaatcatta cttcaaattc
     2161 tgaatcagac tggggacgca tcatcacaat tctacaagat gctggcttag tcaaagtcaa
     2221 agatggagta gatctggaaa cagcaacatt cgatgatatt gaagaaaacc ctaagaactt
     2281 gaagttcaac catacgatcg acccatcact tttagcttct acgtaccaaa atgatgaagg
     2341 tgacctagtt gcaatcaacg caaacttcgc ttacggcgca gggttgaatc cattagaaga
     2401 tgccgtattg cttgaaaagg acaactctcc ttacgtgaat attattgcga ctcgtaaagg
     2461 tgatgaagac agtgacaaaa tcaaaaaact gattgaagtg cttcaccgca aagaagtcca
     2521 agaatggatc acagacaaat ggggcggctc tgttaaacca gtaaaggctg atgcaaaata
     2581 aggtatggaa caaaaaagat gagaaataag tctgtaatgg cttatttctc atctttttta
     2641 tacctattct aatttagaaa tcaaaattgt ttaataataa ataaaagata tttgatttta
     2701 gtaaggaagg tcattgcgct tcattctcaa ttagtctaaa atgagtgaga acgaaagaga
     2761 atttgggggt ataatgcatg tctgtcttag aaataaaaaa tctacatgtg tcgatcgagg
     2821 aaaaagaaat ccttaaaggc gtaaacctaa caatgaaaac cggagaaatc catgccatca
     2881 tgggacctaa cggcacagga aaatctacct tatctgccgc tatcatgggc aatccaaact
     2941 atgaagtaac agaaggcgaa attttgtttg acgggaaaaa tgttttagac ttggaagtag
     3001 atgaacgtgc gcgacttggc ttatttttag ctatgcaata tccaagtgag ataccaggta
     3061 tcacgaatgc ggaattcatg cgtgcagcga tcaatgcgaa acgtgatgaa aacgacaaaa
     3121 tttctgttat ggcatttttg aaaaagttag atgaaaaaat ggctttacta aatatgccag
     3181 aagaaatggc tgaacgttat ctgaacgaag gtttttctgg cggagaaaag aaacggaatg
     3241 agatcctgca attgttgatg ttagaaccta catttgcaat tttagacgaa atcgattcag
     3301 gtctggatat cgatgctttg aaagtcgttg ctaaaggagt aaatgaaatg cgcggagaca
     3361 atttcggtgc attgatcatc acccactatc aacgattatt gaactatatc acacctgatg
     3421 tcgttcacat catgatggaa ggccgagtag tcatgacagg gaatgctgac ctagctaaac
     3481 gtctggaagc agaaggctac gctggtatca gtaaagaatt aggaatcgac tataaagaag
     3541 aagaagcgta agggggagca agaatgaaag aaaaaaactg gctcgattat cttgacgcgg
     3601 taaatgattt ttctttaagt aaaggcgaac ctgattggat gagaaccttc cgtcaagacg
     3661 cactagcgaa agcagatgaa ctgccattgc cgcatatcga tcgggtgaaa ttccatcgct
     3721 ggtcgttatt tgatgtgaaa gaaacacaaa ccatcagtga aacaggcacg attccggcat
     3781 ttgatgcaat gaaagacaat cctgtgcttg tccaacaagg ttcatggacg atttttgaac
     3841 aactgcctgt ggaattagca gaaaaaggag tcatttttac cgacttgttc actgcaatga
     3901 tcgaatatcc agaactagtc caagaatatt atatgaaaaa agcagtaaac atgaacgaag
     3961 accaattgac tgcactccat gtagctttta tgaacagcgg tattttcctg tatgtgccta
     4021 aaaatgtcgt gatcgatgaa ccactcgagt ccttattcat tcaagatggc gcatccgatg
     4081 agcacttctt caagcatgtg ttgatcgtgg cagatgaaca tagtgaattt tcttatctgg
     4141 aacgtttcca gacaaccaaa gaacaagtag caaaatcatc aggtaatatc atcgtggaag
     4201 tcatcgctaa agctggttca aaaatcaaat attcagccgt agatcaatta ggagaaaata
     4261 ttacttctta tatgaatcgc cgtgggcaca ttttacgaga tgcttctgtc gactgggcaa
     4321 ttggtgtcat gaatgacggt catgtgatcg ctgactttga ctcagattta gcaggagaag
     4381 gtgcacacgc agaagtcaaa atcgtggcaa tcagctcggg cagacaaatc caaggaatcg
     4441 atacacgagt aaccaataaa gcccctcata cgatcgggca tattttgcaa cacggagtga
     4501 tccgagaaaa aggaacattg acctttaatg gtatcggcca catcttgaaa ggggcaaaag
     4561 gtgcagatgc ccaacaagaa agtcgtgtct tgatgctttc tgataaagca cgtggtgatg
     4621 cgaatcctat tcttttgatc gatgaaaacg aagtaactgc tggacatgct gccagtgtag
     4681 gacgagttga tccagaagaa atgtactatc tgatgagtag aggattgcat aaagaagaag
     4741 cagagcgctt agtgattcga ggattcctcg gttctgtctt aacagcaatc cctgtagaac
     4801 aagttcggaa agaattagtc gaagtaatcg aagggaagtt gaatggatga tcgatgctaa
     4861 aaagctacgc aatgattttc ctatcttatt tcaagacgtc aatgatgaac ctttagttta
     4921 tctggataat gcagcgacga cccaaaagcc aaaacaagta ctccaggcat tagcgtacta
     4981 ttacgaacac gataacgcca atgttcatcg cggtgtccat acgttagcag aacgcgcgac
     5041 ccatgattat gaacaagcaa gagaaaaagt acgggcgttc atccatgcaa aagaaacagc
     5101 agaagtgctg tttactcgtg gtacgacgac aagcttgaat tgggtagcca gaaccttcgg
     5161 cgaccgctat atcaaagcag gagacgagat agtgctctct tacatggaac atcattctaa
     5221 tgttatcccg tggcagcagt tggcaaaacg aacaggtgct gtcttgaaat atatcgaact
     5281 aacagaagaa ggcttcctgg atatggagga tgcagcgaaa aagattacgg acaaaaccaa
     5341 aatcgtctcc attgctcatg tatccaatgt cttaggtgtc ttcaatcctg ttcaagaatt
     5401 agctgaactt gtccatgaaa acggcggggt catcgtagta gatggtgctc aatctgcccc
     5461 gcacatggcc attgatgtcc aagcactaga ctgtgatttc tttgctttca gcggacacaa
     5521 aatgtgcgga ccaacaggta ttggtgtttt atatggtaaa cgaaaatggt tagaagaaat
     5581 ggaacctgtt gaatttggtg gcgaaatgat cgattttgtc gaattatacg acagtacatg
     5641 gaaagaacta ccttggaaat tcgaagcagg gacaccaaat atcgccggcg ctatcgcatt
     5701 aggccatgca atcgattatt tgcaaaaact cggtatggac aatatccatc gatatgaaga
     5761 agaactagcc gcttatgtgt tgccaaaatt acaagcaatt gatggattga ctgtttatgg
     5821 tccacaagac ccgaattttc ggacaggagt cgttgcattc aatttggatg ggttacatcc
     5881 ccatgatgtc gcgacagctt tagacatgga aggggtagcc gtcagagcag gtcatcattg
     5941 tgcgcaaccg ctattgaagt accttgaagt atcagcgact gctcgcgcga gcttttattt
     6001 ctacaacaca acgcaagacg ccgaccgttt agttgaagca atcatggcaa caaaggagtt
     6061 tttccaacat gggactatct aaattagaca atttataccg tcaagtgatc ttggatcatt
     6121 cttcccatcc gcatcatcgt ggaagcttga ctgactcgac aagcaagatc gaattgaaca
     6181 accccacatg tggagatgtg atcgaattgc aagtcgatgt aaaagacgga atcatcaaaa
     6241 atatcgcctt tgacggcagt ggctgctcca tcagtacggc aagtgcctcg atgatgacag
     6301 acgccgtcat cggaaaatca atagaagaag cagaacattt aactgtagac tttctacagc
     6361 ttgtccaagg aaaagaagtc aaagatcccg accaattagg cgatgctgct atgcttggtg
     6421 gtgtagctaa atttcccgct cggatcaaat gtgcgacact tgcctggaaa gcactaggaa
     6481 aagcaatgga aaacgatggt accgcagctt tccatcatta ccacaaggaa gaggagtgag
     6541 aacatgggcg taccagaatt agaagaatat aaattcgggt ttcacgatga tgtcgaaccg
     6601 gttttcagta caggagaagg cttgacagaa gaagtgatcc gtgagatgtc aagaatcaaa
     6661 ggcgaaccag aatggatgct tgaattccgt ctgaaatctc tagaaacatt taacaaaatg
     6721 ccaatgcaaa catggggccc cgacctttca gacatcgatt tcgatgccat caaatattac
     6781 caaaaaccaa gtgacaaacc cgctcgtgac tgggaagacg taccagaaaa aatcaaagat
     6841 acatttgaaa agattggtat accagaagct gaacgagctt atctagccgg tgcttccgca
     6901 caatatgaat cagaagtcgt gtaccataat atgaaagatg aattcgaaaa gttaggaatc
     6961 gtctttactg acactgactc tgctttgaaa gaataccctg aattattcaa agagtacttt
     7021 tcaaaacttg taccgccaac cgacaacaaa ttagcagcgc tgaattcagc cgtatggtca
     7081 ggtgggacct ttatttacgt gccaaaaggc gtcaaagtcg atgttccatt acaaacgtac
     7141 ttccggatca atgcggaaaa cacggggcaa ttcgaacgta cgctgatcat tgttgacgaa
     7201 ggagcaagca tccactatgt cgaaggttgt acggcaccta cttattcaag taacagcctg
     7261 catgccgcca tcgtagaaat ctttacaaga aaagatgcgt attgccgtta cacaacgatc
     7321 caaaactggt cagacaatgt gtacaactta gtaaccaaac gagcaaaagc atacgaaggc
     7381 gcaaccgttg aatggatcga cggaaaccta ggtgcaaaaa caacgatgaa atacccaagt
     7441 gtttacttag atggaaaagg tgcacgaggc accatgctct ccatcgcctt tgcaggagca
     7501 aaccaaatcc aagatacggg tgcaaaaatg atccacaatg caccaaatac atctagctct
     7561 atcgtctcta aatcaatttc aaaagacggc ggagaagtaa actatcgagg acaagtaacc
     7621 tttgccaaaa acagtgcggg ttcgatctct catatcgaat gtgacacgat catcatggac
     7681 gaatggtcga aatccgatac gatcccattc aatgagatcc acaacagcca agtctcttta
     7741 gaacacgaag ccaaagtctc aaaaatctca gaagaacaac tctactatct gatgagccga
     7801 ggcctgaccg aatctgaagc aactgaaatg atcgtcatgg gctttgttga accattcacc
     7861 aaagaacttc caatggaata tgcagtcgaa ctcaatcgat tgatcagtta tgagatggaa
     7921 ggcagtgtag gttaaacctg ttataggagc tagaaccctt gatatataag gattctagct
     7981 ttttttgttt tgcctgtata ctagaaatga ccagcttttg accagctctt ttactgatga
     8041 gccatcatat cagcaaagtc ttgacctgtt ttcttaggtg cattatttgt aatatgtgca
     8101 tagatgtcta atgtcacttt actattttca tgccctaatc gttgttgaac taccttgaca
     8161 gattctcctg cttctaataa caaactagca tgtgtatgtc taaacccatg aggtgtaata
     8221 cgaggcaagt tatatttttt caaaatatat cttaaccaat cattaacagt gtttggaatg
     8281 tatagagtgt ttttaactgt tgtaaaaaca tgttgctctt tagagctagt attgtatcca
     8341 agttttaaat actcttcttt ttgctgtaat ttccagttgt tgaggataga aagagtctca
     8401 gtatctaaag agataactcg tctagaagct cttgttttcg gagattgaat tataacgtta
     8461 ccaaattcat ctacagcaag agttttattg acatgtagtt ctttattaaa cgtatctatg
     8521 tctttccact gtaaagcgag tacttcactc tttctcatac ctgtgtatgc caataatctg
     8581 aagaaggcgt attgtttcat gttaccaaaa tctttaaaag catccagtaa atcattaagc
     8641 tcctcttttg agtaaaattt acgatttttc tcatactctt tttttcgtgg aagaagtgtt
     8701 ttttccatag gattattgtc aatatattgc attgcaaccc cgtagcgtaa aatttgtgca
     8761 gtctgttttc tcatatagtc gtaactttca tatttactgt gccattcatc taccactttt
     8821 tgacaatatg ggacagagat attcgataat ttaagcttac caaatgctgg aagtatttga
     8881 ttatttgcgt atcttactga aattgcgatt gttgaagctt taacactggt acgctgatga
     8941 gctatccatt cattatacag gtcttcaaat gtcatatcag gtgctttctt tttaacaccg
     9001 tataatattt gatgtctgta ttcgtcataa tctttttgag cttcttgttt gctactaaaa
     9061 cctcttctcg tagtatttat tttttcacca gttcgtggat cggtggcaat gtgtccttta
     9121 tacatccacg ccacttgacc gtttttcttt ttgtactttg atatttttgc catgaaatca
     9181 tctcctctta gtttacaagg agtacttacc actagatggt atcacctccc ttcatttttt
     9241 tcttagtgta ttttttccca attgatgaaa tcgtttgttc tagcttgttt atattatcag
     9301 taatttctct cacagcttct tcgggataag acatgtcttt caattgaata ggtagccatt
     9361 catctaccat tctttctagt agttcttcta tactgctagt gaggaaaaga atgttattgt
     9421 caaattcttc tacaatagca ttgatgaata attcttctat ttttaagata ttgaaaagac
     9481 taattttttc atttttaact ttattacaag tcttgttagc aataaattct tgcatctctg
     9541 gttctaattt ggaatatcta tctagccatg tatctaaatt agagtgtact ggtgggaact
     9601 gttgacgaag atgctggaac aggtttttat ctggaaatgt tataccatct agtacaatgc
     9661 tatctctata aagaataatt cccaaaatat attcttctac attgccacaa actaactcct
     9721 ctatactcat atttctcaaa tcagcaattc gggataatcg ctctggtatt ggtaaatagg
     9781 ttcccttttc ccattcgtag atagtttttt tgtcaatcaa ttgaccgttg ttaatcataa
     9841 gaccaaatgc tggttgtgtt aattttttac tctttcttat agcttttatt tttgttccga
     9901 gtatcttttt tcgttctaaa taatttattg gtggacgctt tatcatattg accacacctc
     9961 ctaaaattag tataccttaa aaaaggggtg ttgtaaataa atttaacaaa aatattgaca
    10021 cccctttttc gtcgaggtat tatgtgtatg tcgatgaact ggtaatcatt ttttgtttga
    10081 ttaatcggtt aataacaaaa tacttttatt ttgagcaaaa aatacgaaga tgattcttag
    10141 aaaatttgtt caagcaatca cgaaaggagg ggaaaatatg tctctacaaa tcattgatag
    10201 cattgtttct atacaagttg aacaacaagt gaaagagata attacagtgc taaagcctat
    10261 gctagaagac attatttctg gagtcactct agagtatatg gactatcagc aggcaagtga
    10321 atatctaggt gtatcagtag gaacgattag aaaatatgtt tctcaatacg ggttacctgt
    10381 tataaaaatt gatacggtca ttagattcaa aaaatctgat attgatagtt tcatggaaca
    10441 gtataaaggt tgagtaaaca aataagacta aaacatatag taataaagag tttgtgttta
    10501 ttatctgtat gttttagtct ttaacagaat tttataagga ggaaacacaa atggaaacac
    10561 caatgattaa tattccaatt aatgtagata aagaaagact attagagaaa ccagttatag
    10621 aggatgaaaa aaagagaata cgaaaaagat gtttagataa ctacagagaa gttacaagag
    10681 atgagtttgt agagatacta aattcatcac aatcatttat tccaagtaag acaaagaata
    10741 aaggcaacaa aagtgaagag tttttagaaa ctagagttat tattctggat gtcgataaca
    10801 ctgttaaaga tgagaagggg aaggtagtag atttaagtaa agatgatagt agatacttaa
    10861 gcatagaaaa ggctttatct atcaatttag ttcataattc tgcgtttgcg atacaaaaaa
    10921 gtattcggta ctctgaaaat ctagaaaaat tcaagattgt ctttctatta aaagaagcaa
    10981 ttactgacta tagtaaaatg gttgctgttt acgaatactt gaaaaaaatc atacccgggt
    11041 gtgatgacaa tgtaaatgct tcgaatagaa tgttttttgg aggatataag tcagatgcag
    11101 tgataatgat taatgaagac aacatgttag atattactga gcttccgatt gattttacca
    11161 tgacatgttc taaaaattgt caaagtgatt cagggagaga gtatgaatta gtcaatgaaa
    11221 ctgattttgt taagttgata aaaaatgacg acaaagaaga aatgaaacag tggtttaaag
    11281 actgcttgtt tgattcctcc gacatgtctt tcaatgagat ttatgaaaga ttgttaacca
    11341 ttgatatgga taaattactt aagtcaagta aaaacttaag atgtttgttt cacgacgacc
    11401 ataatccctc tgctagtatt tttaccagta aaactgggca ttccattttt tattgtcatt
    11461 ccagtaattg tagtatcagt gaaaatttta ttggggttgt gatgttaatt ctaaagaaag
    11521 acagtcgggt agaaacattt aattggctat tagagaacct agacctgtat cctacacatt
    11581 ttcgaaaact gaaagaggaa tcaaaagtac tttttgacac tcttgaatcg atggaagaca
    11641 agcttttcag gacaataagg aattatctag aggaaatgag acatgtctat gatatcttgc
    11701 tttctgatgt taattttttt gatgatagta aggaaagtgt gacttgtatt ttgtcggggg
    11761 aacaattagc taaaaaaatg agttcttact atggtaaaac ttatgacata gataaatgta
    11821 ataagttact atcacttatg acttttatag gattgataaa aaaattagat gatgaagatg
    11881 ttcctttgaa gatgctcgaa tacctaaata ggttaaagga agagaaagcg ttaggaaact
    11941 ttaatcataa gagaagcaat gtctatcgat tagacgtttt taatgcaact tctgtgattc
    12001 aaaaattgaa tgaagatatc cttccacttc tggaaaaaat tcattttaca tatcaatatt
    12061 tttcttatga atgggttaag atatgtttta acgaaaaaga ggctaaaaga gtatttcctc
    12121 aaaattcccg atcagaactc agcaaggaaa aacaattgat tttagatgaa gcacatgaag
    12181 ttttacaggg cttatcgtgg caagggacat atctaatgac agagaaagaa cttattaaca
    12241 ctgtttgtga acgcctacgt aatgttgggg ataacagcgt gaaaaatgct ttaataaaaa
    12301 acagaggcta ctttgttaaa caaggattca ctcttgaacg agcttctaag gatattaaga
    12361 aactatacaa tgtgaaagca agtagtagtt ttttaatata tttagcgaaa ggggcaggga
    12421 tagatgaaac aataaaagaa ttgatattat caggagctgg ggtagttcct ctgtatcaat
    12481 ttacaagtgt aacaagagat gatggacgga gagtaaggaa gagaaaagac ccaattgtat
    12541 taaactagac aggttatcca tagctcttaa tcttatttca tctcaataca cccaccgtta
    12601 aagttcggtg ggttatttat ttttcaatct ttcaatagct gattgcgttc attttcttgc
    12661 ctttttaata gcttcatata gtcaattgtt cgaactgcat cttcagtgct aagagaacga
    12721 tagagagcca aaaactcttc ctcataatct acgacaacat tgtctagaaa atggcttaca
    12781 ggggttccta gggctctaga aagagctgtt aagctatcga ggttaggttc tgttacagaa
    12841 ttttcccatc ttgctactgc ctgttgtgta gagaagattt ttttagctaa ttctttttgt
    12901 gtccacccga gagcttttcg cttttctcta atgacctttg catattgttt ttgtttcatt
    12961 aatacacccc cttctcagta gagtacaaca agttgttgta tttgtaatga tatttgaatt
    13021 tttaatatgt tttatgagtt gaaatacatc atattgttgt atataatgga acaggagggg
    13081 agaattatga aaaaagtaag taatctcagc cgatacagaa aagaaaaagg attttctcaa
    13141 actgagttag ctaaaaaaat gaatgttaca cagcaatgtg taagttcgtg gcaaacaggg
    13201 agaacgattc ctaaacccta tcaaatgaaa atgctttctg agatattaag tgtaccaata
    13261 aatgagctgt tttctgatgt atttaataaa gtaaacagtt aactaataaa caattgaatg
    13321 tttttaggag gagaagacat gtattgtaga aaatgtggca aagaaataac agatgaaagt
    13381 aagtattgcc agttttgcgg agcactacaa gaaacagatg agacaactgg agcagacgca
    13441 gtaaagaaaa gtactgagca gacaattaat gaaatgaagg aacaaatcgt acatggacaa
    13501 gagacaggga agctgtattt gattattggt tggatttcta tggtagtatc gttagcattt
    13561 attcctgttg tatttggtgc tgttgctgtt attatgggtt acttatatcg agagaaagat
    13621 gaaaaaatgg gtacgatttt gatgatagca ggggtagctg gagccatctt tggtgtactt
    13681 ttagggatgt caatgtactg ataagaggga taagagcaaa ttattttgga gataggagat
    13741 aaaaagttga ataaagtaaa tatgaacaag aagtttgtca ttataggtat tgttgtcttg
    13801 gctattatag gtatcatttt ttcaaaaatg acaggtacat ctggattaga gaagtcatcg
    13861 aaaccattgg taacacagat tttacaagaa cagtacagtt taaatcggac atgtgatgat
    13921 gtgacgatta ctcatgaaaa tggagataac acatatcgag ctaaagctat attagacaat
    13981 ggaagtgcta tcaacataaa tattgagtac tatccaaaga aagaccgtat atatgtagag
    14041 ataccatata ctgaggtgtt aatgttgaat tgaaccccac cccattgatg attgaccgta
    14101 ttttgcatgg aaaccttggg aggcataagc taactagggg ttcctttttc ttttcttttt
    14161 ttggaatcta tttaagtgaa atgtatctga aatgaaatgg gagtaagtgt tataatagtt
    14221 aatacataag cgctttattt tatttcagat ggtgaatagt ataaggaatt tttttgatat
    14281 tattacagtt tatcttacta taaagtacta ttttttaaag caataacaga tgaaaatttg
    14341 attcattcat aacaacatgt acagctatac cacctacttg gaataactga agaaaatgta
    14401 cgaaaaacca tttcaagtaa accccaaatg attgttaatg aactacaagg gtggtgaagc
    14461 ttcatgagtc tccacagaga tgctccatca aaagttccgt caagctcatg aaaattgtga
    14521 aaaacgtttt gaataatatc aaagaaagag ggaatggaaa tggcacacaa tgaacgtttt
    14581 aaacaagaaa ttattcaaga actcgagaaa ggagtacaac accaaaaatt aatatcaaag
    14641 accgcccaaa aatttgatat ctcactggct aaggaggtta aacttcatga agtttaacct
    14701 gctagctcta ctaaccgtgg taacgctcgt ttttgttggt tgtactaagg ctcctgattc
    14761 cttgaaaaat tcttctaaca gtgcggaggt aaacgtttcg acctctacca gtgaagcggt
    14821 acagtgttca caagaaacta ctagcttgcc agaagagcta cttggagatt ggcagattaa
    14881 taataaggat gctggtatag agatatcaga cagtaaagct gtattccgta taggaaggtc
    14941 tcaagtagag tcaaaggact attatgtaga tgtaaacaac aaaatgcttt tacatgtcta
    15001 tttttctttt gagtatgatg gtgtgaaata cagtgaagtc tctattgaaa gaactggcta
    15061 tagtgaattt attagcaaaa gcgtgggaat tttaactatg catactaaag atggacaata
    15121 tataagtttg ggtgtgaaga aagtttccgg tgagaataat gagacagaag cagttacgga
    15181 gcagacgatg gatgaaagaa ttgattcatc tgagagtaat gagacagaag tagatactgg
    15241 gaagacgata gatgaaagaa ttgattcatt catcgaaaca tacacagcta taccacctac
    15301 ttggaaactt gtaaatgaag ataaaggaat cacgataaat actgatacag agatttctac
    15361 agatgctcaa tctttatcag atttattagc caacctaaca ggaatcagta atgcggtgac
    15421 agagcaagtt ggtaaaaatg ttccacttcg gttacttgaa aatgggaaaa aagattattc
    15481 tgtggtattt aagaacggac aggtaactga ttacaacgaa gtatttaaaa aagttattga
    15541 ataatgcttc ttcaatcttt ttcattcatt taccccaccc cgttgttata taatcgtatc
    15601 tcaagaggaa ccattagaaa gcataggcta actaggggtt cctttttctt ttgttttttt
    15661 ggaatccgct taagtgaaat gtatctgaaa tgaaacgaaa ataacgtgat tttttactgt
    15721 aatatcaagg ttttagctgt tttagatacc atgtaaccca aaatagttta ttgagaaaat
    15781 aggagagtat gtgatactaa ctagtaggga aaaatgagat aaaaagtaaa tggaatcatt
    15841 tcagaaataa gtgaaataag acaacgtaat aggtataaca tgactattac atagttttag
    15901 acatgaaata ttactacaaa atttgtctaa ttatgtcatt atttatggag gagttttata
    15961 acgatttttg taataaaaag acgagaagga atgcatacat gcacatcctc tcgtctttta
    16021 actatttact ttaggaatta caacaattgc tgtaattcgt cacctattcg ttcaactatt
    16081 ttcgtgacta aagcatttcc cataaagaat aagcgcattc tatctgaaac ttctactatt
    16141 tccccggagg ataatttttt atactttgtc caatcatcag gaaagtcttg tagacgttcg
    16201 gcttctatag gggtcaacaa gcggtaacga ccatctattt ttaataaatg tgtagatcta
    16261 ttcacagaac cttctgaagt tagcatcgtc cgtccgggca tatctaaatc atcataagga
    16321 gacataccac cttcggagaa tgtataggta tgaccttcag cagtgactcg ctctattttt
    16381 ttaggtccac gaagatattc gaattttttt attttacttt cgtcggtcaa gtaatacttt
    16441 tcagctactt catcttcagg ttgaatgatt tctcttaatg gagtaggttt ttctttatcg
    16501 tatggttccg tatctatagt aaagtaacgt ccatgtctca ttaccccagt attgtatact
    16561 tttccagtaa aattatctga tatctctact atgtcattgg ataacttata cgacgcttgt
    16621 ctacccttat aaggagtctt gatgattgga aattgtcgag caaaaagtcc ttcactgaat
    16681 atatactcat cataacgatg ttcttcttca ccaagtattt tttgttcgaa tcgtgcatct
    16741 atcttgttcg caaaaccagt atcatttttg taaacgaaaa aaaagacacg gcgtctacgt
    16801 tgtctacggc cataatcagc agcattgata acacgccatt ctacggaata ccctaaatca
    16861 ttaaaggaag caagcattat agcaaaatct ctgccacgtt gtttagaagg agatttaagt
    16921 agacgatcaa cattctctaa aatgagatac tttggtttta tgtggttcgt agcacggatt
    16981 atttgccaaa atagaactcc ttttttacct tctatcccca tctcatgttt tttactacgt
    17041 gcaactgaat aatcttggca cggaaatcca ccaacaatga tgtcagcgtc catttgctca
    17101 aattcttcat ttgatatttc ttctatattc ctgttgatat tttcactatt tgggaatcgg
    17161 tagtcatata cttcaaaagc atcttgtgat tttcgagaag gctcccattg gtttgcccat
    17221 ttcgttttga ataggtcggc attagaatgc tcgagtccaa ctctgaaccc ccctacaccg
    17281 gcaaaaagtt ctaatacatt tagtgtttca ctcatattgg cgaacatcct ttcggtttta
    17341 acttaaatgt attatacaat aaataaatcg atttgaaaag atcgaaatca aaaatgctat
    17401 gatttactta aggagtttct caaggttatt ctgtaaagtt gaaacaacat caaatgattt
    17461 tgtatagact tggtttctat gtaaccatgt agtatagttt tatataggaa taaacgtagg
    17521 atggagtgac tgaacgaatg tctgaatact taacaaaaga gcaagtacat aatagagcta
    17581 aagaagcagt ggggaagtct atgatagaat taaataatgg aattcccctt ctcaatagta
    17641 aaaataaaaa ctctatagga gacatatttg agtcttggtt cggcaaggta aaggatagcg
    17701 agagtaggcc agacctggaa gaagcaggtg tagagttaaa ggctacgcca ataaaaaagt
    17761 tgaaaaacgg tcagtatagc gctaaagaac gattagtgtt gaatatcatc aactataatg
    17821 aggtcgctga ggaaacattc gaagaaagtc attttttgta taaaaacaac atcatggaaa
    17881 tcgcatttta tgaatactta aaagatacgc cgaaagacca gtggactatc cgtgaagcta
    17941 ttttatatga gatgtataaa aaccctgtag attttgaaat catacgacaa gactgggagc
    18001 ttataaagaa atatatttta gatgggaaag ctcatgagct aagtgaaagt ttgacgaact
    18061 atcttgctcc atgtacaaaa ggaactaatg ctgtgaagtc actcaggaca cagccctatt
    18121 cagaacaaaa agctaaacaa cgggcatact cattgaaatc aggatacatg acatctattt
    18181 tacgtaaata tgtattaggc gatgaaaaaa tcgattcaat cgtgaagaat acttttcaac
    18241 ttgaagaaaa caacttagaa gatttagtat tagaacattt tataccatat gtaggtcaaa
    18301 ctgtacaatc tttatgtgaa aaattcacaa tcaagacatc gtatcaagct aattatagaa
    18361 tcgtttcttc aatactaggg ttatctggta ctgtaaccaa aggggaacct ttcccaagag
    18421 tcgaagaatt tgaaaaagcg tctatactag ttaaaaccat atattttgat aaaagtggaa
    18481 ataacaaaga aagcatgtca tttccatcat tcaattttat tgatttgagc aaacaggtat
    18541 gggaaaaaga agatggggaa ccaagcagtg attggcataa ttttttatta gatacaagat
    18601 ttctttttgt agtctttaaa gaagaagatg aaggtgttgt attcaaggga gctaaatttt
    18661 ttagtatgcc agaagaagat atagaagggc cagtccgtaa ggtatgggaa gatactgtgt
    18721 ctaaacttcg agaaggagta gagttagaag ctattcctga taaatctact aaggatggat
    18781 ggaaaatcaa aaataatttt ataaagaaaa cagacaaaat gatttgccat gttagacccc
    18841 atgctagcca acgagattac tctgtcgatg gacgctatgc ggataaactt cctgtacccg
    18901 caaaatggat taataaacca gacaacgata attactcgga tcaatggatg accaaacaat
    18961 gtttttggat aaacaatgat tatataaaag agcaagtaca agatttgata tcataaaaaa
    19021 agaggaagac tactcctctt ttttttgtgc aatataatct aatatcacag atgtgcaata
    19081 atttaagttt ttcaaaatat ctttttccca aaagtgtata ggtacccaac caagttttat
    19141 gagagactga ttattccttt catctctcct catattttct tctatttttt caatccagta
    19201 gtcccgattc cgtttcaact tttgttttct attgtcccaa tcgtgtccgt gccaaaactc
    19261 tccatcgacg aaaattgcta gtttatattt ggtaactgct atatcgggag agcctggtaa
    19321 ttttttatag ttttttcgat acctgacacc ttgatgccat aactctttgg ctagcagact
    19381 ttcagctttt cctcccttca agcgtacatt tgccatacgt tttgatactt cgggagtggt
    19441 cgatagttgt ctatgcttca ttgtttttta cctccaatgt tctaatagaa tcaagaaatg
    19501 ttagtacaga gatatgattt atttaagata tcactatcat aaatatatag ctaaatgaaa
    19561 aaagttgcga atatggtctt atagaaatag taattatttt cagttctcag cggtcttatt
    19621 gattccgttt tattgatttt aaatgttatt cagtgaccct ttaacaggtc aatttttatg
    19681 tttttgtctt cgtatgactc agtttgatat ctagtgatat agtgatttga gtctacatcg
    19741 aacaatggaa taatccaata aaaaatgatt tagaatatca aaataacaaa aaagaggact
    19801 acctgtatta gtcctctttt tgaccagatt ttgaccagtc tattttacat tctaatatat
    19861 tcatatgagc taattttatg agtaaatggt aagtttttcc ttgtttgttt tagtttaaac
    19921 tagtgtgtaa tttaaaaatc tatatggaag ggtctgtggg gtaatactta aataaaggct
    19981 aggaacctga taacatcaag gttcctagcc tttttataat aaaatgaagc gaaaataatt
    20041 tcttttaaag agccgagatt atcagctata taagagtgaa tttgatgcaa aaaacagagc
    20101 cgaaacagtc ttggtgttcg tagctctgct tatatgtttc gaatttaaga caaagagttg
    20161 gcaaaagatg ttgataagga agataagata actgtgttat ataaactagg taactaactg
    20221 taacaagcta attttaagag attcttatta gaagacgttt aggaatataa ctaagaaaat
    20281 gattatacag gtaattgtca tttcaataat gttttctaat tttcgcgtct ttgttttaag
    20341 aaaaaggcca gaatcagttg gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc
    20401 tactaatatt cccactaaca agagataaaa cggccaggtt ggtgtgattc ctctataaaa
    20461 aaatgcccat gctaccgaaa tcccatagat taacaactga acaattttta caatgaaaaa
    20521 attgttctgc cattactcaa agtctcttcc tccttgtatc gtttatttgt ttcattttat
    20581 cattatgata ggcgcttgaa caatccattt tgtattttat tgagtactct tggataaact
    20641 accagcgatt atcatattta tttatgttac tgtaagccat catataaaag taaatgagca
    20701 gatcatagaa atataaaaaa gaactaaaat tgatctaaat tatgtgcagc ttgactacgt
    20761 accaatctat attacttact agattggtcg ttaatctatt cggatcgaat gagcaaaata
    20821 tttttgtgaa tgtccatcct tgattatgat tccaaaaaga atcattggga ggctgtggcg
    20881 taaatattat actataagcc agaaatacct atcattctga acttttttat ttaggaactt
    20941 tcatctcaag gctttttcaa ttaatttgta cccctgtttt ttggataaga tcatgtgcat
    21001 tgattttttc tatctctacg ttcatctcct acttaatgcc tatctggctc ctgagggctt
    21061 ctttttaagg cgtcgtcata gtcttggctg aggatttctt ggtaatctga gggaatggct
    21121 tcccaagtga gcaggcgaac gttcatgacg tgttttcctt tgtgtttgat tacagcatgg
    21181 tttttctctt tttctacctc tccagcgtga aaagacagcc atgtaccatg gccaaagata
    21241 tcattacata cccaacgttc gatggaattg tccacgtatc ctacagggag tgtggtatag
    21301 ccgatttgtg gtttcaaaaa aggattgaat cgatcgatcg tttcattttt gtattgataa
    21361 ttgataagaa aaagatagac caaacaaaag acgagcagaa agaaagatag tgtctttttg
    21421 agtgagaagg gttgttttga tgaataagat ttcttgttaa caagaggttc atctggcaat
    21481 gttggttcta ttgattcggt agctgattca atagaaggaa aatcaatcgg cagacctaaa
    21541 gtatgatgta aaagtgacag catttcaagg tctggcattg tacgagaatt ttcccaacta
    21601 gaaatcgttt tatcggaaac accaattttt tcgcctaatt ctttttgcgt aagcttacaa
    21661 tttctgcggt aagttcgtat tttttctccg gtgttcatct tcttctcctt tcgattctac
    21721 agtatataga tttattctac atgacctata cacttgatag atacgttttt ttgatgaaaa
    21781 acggtatgat tttatcaaag aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa
    21841 gttcacagtt ttactcacaa tcatagtgac cggtatcatg agtggttttg ccttggttag
    21901 tataaataaa caggtactct atcggacaaa tgaatttttc catcaaacag atacttatac
    21961 tccgattcga ttgccacaac attttattgg gcaagaagaa tatgagcagc taatcatggt
    22021 atttgaaaat gtctcatcag aaactgagct ttcttatatg aaaaaaacag taaatgaatc
    22081 caatgaagta gatacatctt ggaacttcaa gatacagcca aagcttgaaa tgacattgta
    22141 ttatcaaggg gatgtcaaat cttctcacga gaaggtaccc tattctaata cctcctttcg
    22201 cgtaacgtcc attgaagaat cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat
    22261 gacgagagaa aaagaagcct atgaacggtt catttcacta ttcactgatc gattcaatca
    22321 agcatttcac actgattatg ccactaatat ttttgaagac tttgaaaata gctattcgta
    22381 ttcggttatt tcaaatacag acgtatataa ttatcgagtg tatttaacta ttggattgat
    22441 tcttttattc cttttgttat catccttttt cttattgatg aataaagaaa tcagtttatt
    22501 gttgaataat gacttttcat tacatcaagt attgtggtgt ttattgggga aaaagaaaat
    22561 cattattaca ttcataattg caggaatcat tggtggtttt atttttatgt attataagga
    22621 gcaatttctt tatttgttcc agatgtacgt tttcttgttc agcggaatat atggtatctc
    22681 tttgatttct cttttattag ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat
    22741 ccaatacatt ctttttacat ttggattgtt gttttctctt cttttgaccg ttgccaatgt
    22801 cgaattagca actatcgttt tttcttcttc acagttagct gtttcccatg aaccaccaaa
    22861 agaaatcgct ggtgaggact atcatgtatt ttatccagta accatcggga aaaaccacgt
    22921 ggattttatt tattccaatc gttttgcatc agcagcagat caggaattat atgaaacttt
    22981 gaataaaaac ggaagtatat tagtcaatgt atcagattac ttgaatgaag aaaatgaaca
    23041 gtttggcaga gggataaaaa taaatctgaa ttatcttaag aaatttcctt tgattgatgt
    23101 ctctggaagg ttgatccaaa taaccgaaaa agaaacgcac cctgttatat tgatccctga
    23161 acgatataga catacagatt taaaaaatga cctggcgcaa ataacagaat attatcaaga
    23221 aatcagtgaa aaagaaccga aatttctatt tataaaaaac aatcaaccca tttatacatt
    23281 tattccttct atcccctgga tcgaacatta tccggttgtt gaaatactga cattgaaaaa
    23341 cagtatgtat ttggaaagaa atattctcag cggagaaatc tatccaccgt taaagatcaa
    23401 gataggctct ttgtcgaaag aaaggttatt tgagctgatt gaagaaagtc agttacgtga
    23461 taacttgcag ttatcatttc cgtatactca gactgaagag atagctgtca aagtgttaag
    23521 cagatcattt cattacttga tacagatatt tttactcact ttcttttctt tcttcttgtt
    23581 gagctatgtc atgttatgca tatattttgt ctacaattgt cggaaaatcg ctatctttcg
    23641 ttcaagtggt tattcgctct ttgaaacgta taaagatttc ttcatgatga acctcatcaa
    23701 atggggaacg acaagtgtaa tcttcctttt tcttatagaa agagagccta aatatctttt
    23761 taatattttt ttctttagct tcattgattt tattctttca gttgttttta ttttgtctac
    23821 agaaaagaaa agtcagttat tgctgatgaa tggaggttaa caattggagc cgttgatagt
    23881 gataaaaggt gcgaaaaaaa tcattcgaaa caaaacgatt cttcatttgg atcgtttcac
    23941 tgcggatgct ggggatcttg tgactgttgt tggagaaagt ggagcaggta aaagtacgtt
    24001 attgaatata ttaggattga atgatcagtt cagttcagga caattctatt tgttcgatca
    24061 agagacgaaa caattaactg ggagagagaa gcgcttgata aaaagaaata ccatatcttt
    24121 tctttttcaa gattttgggt tgattgaata tgaaacaatc gattttaatt tagatattgg
    24181 attgaagttt tcgaatttaa atgtttcaga taagcgaaca gcaaagtatg aggcattgaa
    24241 aaaagtaaat ataaacaaga agctaagtac gcctatctac acattatctg gtggtgagaa
    24301 gcaaagaatc gcctgtgcga gagtcttatt gaagaatagc aaaatcattt tagccgatga
    24361 accgactggg tcattggata aaagaaataa ggataaagta gttgaaatct tatcgcaaca
    24421 agcttcccaa ggtacgttag ttatcgtagt cacgcatgat ccatatctga ccaaaatagg
    24481 aaacaagaag atcatgcttt aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc
    24541 agttattatt tgtgcactag ggataggttt tgttggagga aacgctatgt catctgaagt
    24601 ggaagcaaat tcagttcacg gtgaaatcta ttacaaatct ttaaagccag gagagattca
    24661 ttttgatgct aaaggtaata ttattaagtc aaatttacca gtagaccggg aaatttctag
    24721 aacagcaatt aatcatagct ctggaagatg ggtttattat agtgatacaa cttgtacgag
    24781 aaaaacaggt cattctaacc actatagtcg tgtctataat cgtcacggtg caaaagcgaa
    24841 agttggaaac tctattcgat cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc
    24901 gacaggaaat agaactgaca cattctcatg ttggtacaat ccaaccggtt ggtattaagt
    24961 atccaaaagt ttatataaaa tagaaaaaac atataatgaa acagcgttaa aagttatttt
    25021 tggtagtttt aatatattca tttttagttg tataaaaata tatcataata aaaacaacaa
    25081 aaaaatgtct ggaaaatcga acataaaagt gtctgatttg aatatcccag cagcggaaga
    25141 agaaaaacaa aatcgataac atttgttcta agaattagtt acgttaaata cggaacgcaa
    25201 gatagacttg aaaaacggta acaaaagaag tagtatatta aggtgtgatg tgctacttga
    25261 catttattct gctgagcgaa ctttctggat tgtgtagcct tatatctatt caggacgtat
    25321 cgagcagatt agtaaaggtg ttcttgatga ttttcatctt gaacaccttt gtttttagca
    25381 attcgcaaaa aacgaaaagt aggagaattg ataaatgaaa aaaagatttt tagcacttgc
    25441 catcgtacta ggcactggct tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt
    25501 tagttataaa ggcggtacaa tcagcgaaca agaagtaatg gatagcttga aaaaaatgca
    25561 aggagcagat tccgctgtgc aacagttgat cgtttatcaa gtatttgaag ataaatatgg
    25621 agatgacgta tcaaccaaag aaatcgattc tcaatatgat caaacaaaga aacaattggg
    25681 tgattcattt gattcacaat tgaaatctgc aggttacaca gaacaaacat tcaaagacag
    25741 tatcaaacaa agtctagcct ttcaagaagg tctaaagaaa catatcaaat tgaccgatga
    25801 agatttaaaa acggcttggg aatctttcca tccagaagtg gaagctcaaa ttatccaagt
    25861 agcaagtgaa gatgatgcaa aagatgtgaa gaaagctgct gataagggag acgatttctc
    25921 taaattagcc aaagacaagt caactgatac tacgacaaaa gaagatggcg gaaaagtgaa
    25981 atttgattcc actactacaa cggttccggc agaagtcaaa gaagcagcct ttaagttgaa
    26041 agatggtcaa gtttctgatg tgatcacttc taccaatgct tcaacttata ctacggaata
    26101 ttatgtggtt aaaatggtga aaaaccaaaa caaaggcaac gacatggata aatacaaaaa
    26161 agaattgaaa gaaattgcaa cagacacaaa attaagtgac agtaccttcc agaataaagt
    26221 aattggcgaa gtattgaaag acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt
    26281 tttgagtaca ttcacttcag attctagtac agcaagtagt aaataagcgg tataaataaa
    26341 aaatatgaac tatttaaatc aataaaactg ttcatctaaa tctttttatt agaactaaat
    26401 caacagtagt ttaattacta cttgatttag ttctttttat tttaaaaatt ttttttactt
    26461 agaatccagc gaccctagat atttttctgt ataatcttaa ataaagaaaa caatatcaaa
    26521 aaatgcacgt agaagtagat aaatggagtg gttttgcaaa tttcttttat ttctagaagt
    26581 tattcacgta tacggatttg tttttctcac aaataaaaag gcaggaagta caatggatca
    26641 tttgtatata aaatatattc atcaattttc cgttttggtg ttaggcacac taattatagc
    26701 ggggattgtc gcatttatta ttcaaggccg tgacatatta acagtacatc aagagttttt
    26761 cagttatgca tttggtattc ctctgatcgc tataaggtct gataacaaaa agtggaagaa
    26821 acgctattac attctcttgc tatttttgtg tttttatctg ccacctaaga tttttacttt
    26881 attaggtata caatgcctat cattttcttt tatttcctat atcattgttt taattttcct
    26941 cagtttcata gtatggatgt tttatcttgt caaaaaataa atgaatgttt catgaacagt
    27001 acttcatcac agcctaattt ttttctttat gctatcgtta tattaaagaa atcaatcaga
    27061 agggaaagaa agtgatgaaa aaagttttat tagtggtaaa ccccagttca ggcggtgaac
    27121 aagcaaagga atttgagcaa ttagcgatag caaaattgga atcggtgttt gatgaagtgg
    27181 tcgtcttgca tacaaaaaaa gcaggggatg caaaaaactt tactcgcgaa gcggctacgg
    27241 aagggtatca tagtgtgttt gtcatgggcg gagatggaac agtcaacgaa ggaatcagtg
    27301 gaatcgctga acaagaacat cggccaaatt tcggattttt cccattgggt acggtgaatg
    27361 acttggctcg agcattaggg ataccgctag aacctgagga agcgatcaat catttttcga
    27421 ttgaatcagt caagccattg gatattggaa aaataaatga cgactatttt atgaatgtcg
    27481 tcgcaattgg ctctatccct gaatcaatca atgatgtaga tgcggagaag aagacgaaat
    27541 ttggaaaact agcttatttc atgtcaggaa tcaaacagtt agcttccaca caatcctact
    27601 cgtttcacgt agaagttgat ggaaagaagg aagaaattga gagcagtacc ttgctgatag
    27661 gactaactaa ctcagtcggc ggttttgaaa cattacttcc aaatgcaaaa gtggatgatg
    27721 gtaagctcca tttggtttat ttaaaagatt cttctttgat cgatacgctt aaaacattgc
    27781 cagacctatt aaaaggagta gatgaatcta cgaaaaatct agcctacctt acttgtgagc
    27841 aattagttgt gtcattagct gatgaggaag aattgacgac gaatgttgat ggagatgaag
    27901 gggataaatt acctgtgaca atcaaagtat tgccctcaca tttacaggtt tattgttaaa
    27961 atggaggttg tgaccgagga gttggcggga gaacaccgct tgttcgggat aagggactgt
    28021 gacaagactt ttgtcacagc cttttttttg tacaaaaaag aaatttacac ctaatagaaa
    28081 aatttttcca taaaaataca tattttaaat atgaaagtac aattggaaat agattaaaac
    28141 gataaaataa aattgtcaaa acgtcagaga ggatggtgga gaatgaatca atgtgtaaaa
    28201 aagctgattg cgtcgatttt gtttgtcttc cctctcttga tggtgacatc cgtttttgcc
    28261 gatgtagaga aaacaaacga atcaacgcaa tcagagaatc aaagttttga caagactact
    28321 ttttcagaag aatcgattac ttctgaaact acagaatctc aaagtgaggc ttctactaat
    28381 aaaaggcaag aagaaaataa gacaagtgag acctcagaag tagctgaaca aacgtctgat
    28441 agcagtgaag cggcactggc aagagctgtt tcagcggatg ggtacagcga agctgccaca
    28501 acagaagaac tggcgcagct tctcgcagac gaatcagtaa ctaagatccg actgattcag
    28561 cctttgactt tagacaggga attagagatc aaacgagata tcgttattga ttttggtgga
    28621 ttcgctcaca atttcggcac acaccatatt tatatcaatg agatacctca gtcgatcgag
    28681 ttccagaatt ttaaaggaac agctgcacat ccaggtggat tgacaccaaa tatcgatgga
    28741 aatgccatta tcattgctta tatgaccgat ttttggggaa gcagatatca ttttacggga
    28801 gaaataaaat ttaccgggac acttgatttg tatgatgggt ccaaattagg actgatttac
    28861 gctcctcgtg caactgtcac attagatggt gtttccggtg tgttggatgt ccaaccagtg
    28921 aaggaaggac tgaatgccgc tccgggtaaa gcctattttt gtagaagcta tcaattgaat
    28981 gtgatcaatg gtagtcaact ttatggaccg tatctaggga aattctacgg ttttctagat
    29041 tcaggagatg agactggtag tggaaaaaca gcgccaggaa tccatatctt aagcggttca
    29101 aaggtctctc ttgattatga tcgggcagat ggcgttagcg atgaaggaga ggcagtcgat
    29161 acgctacctg gtaatgtcat gtttgaagtc agtggttcgg gaagcgaatt ttcggtaaat
    29221 acaaagatca atattaccaa tgacaacaat cgcggaatca tccaaatgag aggttcagga
    29281 tcgaaagtca ctgtatcgaa tggtgggaaa atcaccatca atacagcaac tacgggcgga
    29341 tttcgcttgc aaggagattc tagtcaaatc catgtatcaa gtggtggaca aatctcggtc
    29401 aaaatggctg gagatgacga tcaacccggg aacaatggga tgcgttttgt aggaaaaggc
    29461 ctttctttga tcgtagaagg agccggatca gttatcgaca tcgataagca gagtgggcgt
    29521 tccagcgcag ttcgttttga gaatggtacg caaaagctga cagtaaccaa tggaggctca
    29581 ttgagggtaa gaaatacagg tgatggcaaa tcttacgtga acagactcaa tcaaggaaat
    29641 caagcgatcc aattttatga cgcttctggt tttctagaaa gtccaggttc agctgatttt
    29701 acagtcactg gagaaaaatc aaatatcgat atacgcgcag attccggagc aactgtagat
    29761 acaactggag atattgattt aaattttacg gcaggtgaaa aaacttattt agtcatggtc
    29821 gggaaaacag gggaagataa gaccggcatc atcagtgggg atgtgttgaa tgtggactta
    29881 gaatctccta cctattttga tttccaaaac aaacggattg gaagcgagac caatggcata
    29941 ggcggctggg tattccaagg aaatggagat tcttctttaa agatcgccca ttcagaaatc
    30001 gctttatggc gaaaaacagg cgcaacattc gataactttg aaaaagaccc ggattatttt
    30061 tctgatatga cagacttatc gatcaatggg accgacttag gcaatttcgt atcaagccct
    30121 agtgcaggtt taacaaatga atttgctcag acaggtgccg gaatgaaaaa ttataatcga
    30181 atcagtgcca ataatcagct cccgatgatt gaatccttaa gagtgccaac gaatgcagat
    30241 aaaaaaatct atgggcatgt cgttgttccc gaaggtgtcg aggcaaagcc aagagatgct
    30301 tggacaggcg aagtagaagt gactttacgt attacttacg cagatacgac gaaatcatct
    30361 gtagaaatta ctgctctcac gcaaggacaa acagatgaaa atgaaggggc aggatataac
    30421 ccatggggag aaggcgaaca aggcggcttg ttcgaagttc aagtacccaa tgatgagaga
    30481 ttaaaaacgg aggatcaagt gcaagttgtt gctgctaaaa aagtaagagg tggccaagaa
    30541 gtgacagaac tggactctgc caaaacgaca gtcgatgtga ttcctcctga acctgctaat
    30601 ttaagtacca cattgatcaa tgcagcttct cgcacgatca caggtacagg tgaagcagga
    30661 gccttggttt cattgaaaaa aaatcaagct gttttgtcag aaacaactat tgatgataca
    30721 ggggaatttg ttctttctat cccagaaaat caactttttg caggagatgt attagaaatt
    30781 gtattaaacg accaagcagg agaagctact gctaagggtg tgcagaacaa gccaagtacc
    30841 aacgatgaaa ttgggaatca caatccaagt gatacgccag ttgtttatca tgatgccccg
    30901 ccttttgcac ctgctacgaa gatcgtagta gaaggtggtc tctcatttct tgccccttcc
    30961 aaactcgatt ttggtcagat caaagcaact ggtttgacac aagaagcatt tggccaaatg
    31021 gatatacagg aaaaattatg gatttatgat cagcgagaaa cgaaaaatcc gtggatctta
    31081 acgataaaat tatctcagcc tttcacaaat gatgcaggat tttctatgca ggatgtccta
    31141 tatttcaaga aaggagagga gtatcaatta gtgaatgaag aagaactgga aattgtcaga
    31201 ggacaaaatt caacgaatgt gcaagcagat tattcagaat atttaaataa cggtcgggcg
    31261 tttaaacttg tcttaaccaa agaaaatcag atagctggta attactcagc agagataaca
    31321 tggacattag cagatgcgcc gacagaataa agatcaagga ggatgattga tgaatcgaat
    31381 atggaataaa agtcatcgaa acaactgctc tttacttatg atccttctcc ttggattcat
    31441 cctttggcag acacctgtcc atgcctccga attcaatttt gcggtagatt ttgaattacc
    31501 ggctaatcag attgataaga acaaaacata tataaatgta aggatggagc ctgggaaaac
    31561 acaggaaatc ccctacacgg tctttaaccg aacagataaa tcagtaacag ttgagacatt
    31621 gatccactcc gctacgacga attcaaacgg tgtgatcgaa tatggagaat caaaaagtaa
    31681 atttgatgaa acattaagtt atgatatgaa aaacttagca aaaacagtgg gagaaataac
    31741 tgttcctcct caaagtgcgg tcactgagac actttcactg aaagcgccag aaaaagagct
    31801 ggctggtgtg gtcgcaggag ggatcacgtt tcaagaaaaa aaagagaacg tttctaacaa
    31861 aagtacagtt ggtgtcgtca atcagttttc tcaagtgaaa gcaatcttga tttgaaatag
    31921 cgaaaaagag tcttccccag atttgaaatt aaaaaaagct tatgcggatc aaagcgatgg
    31981 aagaaatgtg attaaggcga atcttcaaaa tccaaatgct tcttatatgt tcggcttgac
    32041 tacatcggtt gaaatctaca aaaaagaaac aaaagagcct tacctttcaa taaaaaaaga
    32101 aaaaatagat gtagcaccca atacaaattt tgatttactt gtcccattag aaggaaaaaa
    32161 attagctccg ggacattatc aagcggttgt gaagggtgct tggaaagaaa aacgctggaa
    32221 tctccatacc gattttgaaa taactgatga tgcagcggac caattgaatg aaaaggatat
    32281 tgatttaaaa aatcagccag aaacatttga ctggcgatgg ctgatacttg ttggagtcat
    32341 tcttattggc ttatttgctg gattatttat tttaaaagaa agaaggaaaa agaaatgaaa
    32401 aaaacaattg cattaagtac cgttctagct ggaagtctat tattatgtac acaaagtgtt
    32461 tttgctgaga ataagagctc agccactaca gaaggaacga tttctgtgac caaaccagat
    32521 gctgcaaatc ctgaagacgt tgttccagtt cctcctgttg atccaaatga tcctgatact
    32581 ccttttacac ctgacgaacc aaatcaggga acatccggat tactgacgat tgaccaagct
    32641 ccagatcttg attttggaac aatcaagtta ggtacagcaa aaacagtata tgctgcatta
    32701 caaagcggaa cggacagtac tggagcacaa acacaagtac agaatttcgt acaagtaacg
    32761 gataaaagcg gaaattatgc cggttggaaa ttatcagtca aacgaacaga atttattggt
    32821 acaacatctg atacgaataa actaactggt tcaaaggtga aattcaaaaa cgccgtgatt
    32881 cgaaatagcg caactaatag cagtggaatg ccatctaccg tttctggaac tggcgctgaa
    32941 ggaatcgaaa tcccagcaga tcaggcagta gatctagtaa ctgcaggagc aaatgaaggg
    33001 attggcacat ggatttattc attaggagag aatgtggaac aagggagaca atcagtggaa
    33061 ttatttgttc ctgtatccaa ttatgctgta gacaactacg tttcaacatt cacatggtcg
    33121 ttaacagatg ctcctacaga agaggaagcg taaaatcata tcaacgattt cagggagtgg
    33181 agccgattca aggcccactc cctgaaatct gctatgctta cgtctcagct cttattcatc
    33241 aactcgaagc agttttttgg aagtcatgga aacgttcctt tgtcttttca aagatctttc
    33301 gaccaacgag taaggagtca ccgttgaaaa attctaacac ataatgagca gaatcaattt
    33361 tctgcaattg cgataagttg atacagtaag attttaaaag taaaaagtaa gcatactttt
    33421 ctaactctgt cttcaattgt tttaaagaag tattcactaa tttatcttct gtagttgttt
    33481 tgaccataac ttggtttttc aaacctttga tactttcaat atataaaatt tcccgaggat
    33541 acaggacgag acttccacct ttttgatcaa taaagatttt atcgacattt acgatctgct
    33601 gttctttcca attgatctgt tctagcactt gacgaatttg ttcagataaa ttcctgtctt
    33661 ttctgatata tccaatcgga aggattttcc gattgatgat ctctactgca aaattcgtat
    33721 aagcggtgaa aaaaacaata ttactatgtg catttttttt acgtatttgt tcggcgacat
    33781 caatacctgt gagtgattgc ttcaagtcga tatcgataaa gaaaacatcg tgattatcaa
    33841 tagtgcccaa ctggatcaac tgattgattt gagagataga agtgagctgc acgatattct
    33901 cgatatgatg attgaccaat tctctgatga tccattcgct gtgaaactgg ttatcttcta
    33961 tgataaaata ctttttcata tgcaggctcc tattaaaaaa atatgtgtac attttgaaac
    34021 aaaacaatgg aggaataaga tggtcgcaac agtgacactt tatttgacct ttactttgat
    34081 tttagattta ggtatctatt tattcttttc gaaacaactg ctagtaaaaa acagctttgg
    34141 ttgtatagct ttgatggtgg tgacactttt acttcctatc cctaaagtga gctttcctta
    34201 cctagttggc ttattattga tgtttctttt ttattttact acgaaaaacc tgtttatcag
    34261 ctgtttgatc agtaatcttt tttttgctat cactggactc agcttttttc tggtttttga
    34321 tctgatggat tatttaggaa agagacatct tggaacagca agtttttttg cactacttat
    34381 cgcatttatt ttctatctat tagtaaaaag tatagacaaa cagacgagat ttctcaactt
    34441 gctttttact tatccaactg aacactattt tggaggtatt tgtattttat taagctgggg
    34501 cgtgatttac ttggtattgg gaagtgttca cttccgctca tctaattatc tgcttctatc
    34561 tcttttgtcg atagtcatca gttttttttc tttgtttttt tccattatgt tgatcagttt
    34621 ccaagtaaaa gaaagacaac atcaggaatc gcttcaactg tatcaaataa atgaagaata
    34681 ttaccatcat ctagaggaat tcaaacatga ttataaaagt ctactgtttt cattaaaaat
    34741 tactcttcag gaaaataaaa aagaaacgtt ggaattttta gaaatattag aagatcattc
    34801 tgcagctatt tttgaaaaac cgcaaataca acagctaaca aatattgtat ccccagctgt
    34861 caggggtgtc tttttaaaat ttctagaaaa agcagaatcg gagaaaatcc cgatccaagt
    34921 agttattcct gtcgaagtga aaacaatccc gattgatctg gttgttttta ttcgctgttt
    34981 atctattttt atttctaacg cattcgatca tcgtgtagcc gatcagagtc cgattacgat
    35041 caatctgctg gaagaaaaag agggatcacg ttttagtata gcgaacaagg caaatccaac
    35101 gaatttgacg ctatcggaga tggtcaaaag aggaaaaacc tctaaaaaaa atggcgggtt
    35161 aggtctatac agtgcgaaaa aaatgttgga tgtttacgaa aatgcagaac taaaaatcga
    35221 attttcaaaa cagaatcact atttttttgt agaaatgtat ctggcatcta gaaagagcaa
    35281 atcagcctac tgacttactg tcaaaggata agtttgagga aagtctacac ggagatagag
    35341 acaacaacac agcatattca aaagaaacag aagcgtgatg accgttgtca cccttctgtt
    35401 tcttttgcta tgtagttaaa caaagtagtt gacacttccg ctacttagta ttagtacgaa
    35461 agtatacttc atgatactaa gtagataaga agtgaaaagc ggaacccacg atcgtcaaaa
    35521 tgctaactgc tttgcttaca ccagataaag ggaagagcaa tatcgcttat tttaggcgtg
    35581 gagaagcaag caaatcccta cgatcacaaa aaaaatgctg acccagcagt taagagagtt
    35641 agaacaagat gaaagtatcc atcgaaaagc ctataaccaa gtcccgccta aagtagagta
    35701 ctcgctcacc gaagaaggaa aatcattaag agagattcta gtagcgttgt ctgtttggga
    35761 agaagaacgt atcgaaaaac aaaaagcctc aagggaagaa gacacgctac tgcatacaca
    35821 tggtggctac ctaaattatt gattttctat gacatttatt ttaaataaaa gaaaacagga
    35881 gtttttttaa aagcatcatc tattttttat gtaaaaaaat ggtatgatta atcaagtaag
    35941 aagtgattgg aagaaggcaa gcaaatgaat agtatggaaa aagtcaacca gtttagagat
    36001 gaacgtaatt ggcgacaatt tcataacgaa aaagatttgg ctatctctat ttctttggaa
    36061 gcatctgaat tgttagaact ttttcaatgg aaaacgccgg aagaagtaaa agaaacacaa
    36121 ttagaacgaa tcaaagaaga actggcagat gtattgatct attcatacat gatggcagac
    36181 aaccttcacc tggatctaga tgagatcatt gaagaaaaat tgatcaaaaa taatttgaaa
    36241 tatcctgtag aaaaaagcgc aggtgtacga aaaaaataca cagagttgta gaaatgtgtc
    36301 attcggctgt tggaaagagc aaaagaaaaa cagtaaaatg agtctggagg gagagaaaat
    36361 gaaaaaaaaa ggaaaagcaa aaagatggct agtcaatatc ctcttgttcc tcttgttatt
    36421 agtaggacta gctttgattt tcaatgaaca gatcaaggat tattttgtca gagagacagg
    36481 agataaatat gctatagcga atgtaacaaa agaagatctg aaaaagaata acgataaaga
    36541 tgtcagtttt gactttgatg cggtagaacc aatgacgaca gaaggggtca tgaggtcgca
    36601 aatgagagga acagatctac cagtgattgc tagtatcgca gtcccttcag tttcaatcaa
    36661 tttaccggtt ttcaaaggct tggataatac gtcactactt tacggagcag gaacattatc
    36721 tccagatcaa gaaatgggaa aaggaaatta tgccttagca agccatcgtg caacgaaccc
    36781 tgaattgctg tttacaccgc tagaaaattt agagatgggt gccaagatct atttgacaga
    36841 tttagaaaat gtgtatacat acaaaacatt ttttaaagaa aaagttgctc ctacagatac
    36901 ccaattactg aatgaagtcg agggaaaaga aattgtcaca ctaattactt gtggcgacat
    36961 ggacgccgtc acgcgactag ttgtacaagg agaactggaa agtgttacat cgataaaaga
    37021 tgcgacagat gatatgagat ctgctttcaa tctagagaca aaaacatttt aggatactgg
    37081 gacataagtc gttacagatt aaaaacagcc aaaggatatg aatcgataag gaaaaaccaa
    37141 tttgctgaaa aaaagagaaa cgcaaagagg tgcatctctg atttggttgt tttctagata
    37201 gacttgtgtt ccagtttttt tattttttat atgaaaaggc ttgcataaat aaaaattcga
    37261 tggtatgatg attttgaata agaaatggat tgttactgta cggcaggcaa aaccagaatt
    37321 cagctaagaa acagtaggcg aattctaggt ttttttattt ttcttgagag ggcgaagcaa
    37381 aatgaaaatt gataaaattt tgaataacaa tgtcgtcatt tcgaaaaatg gattcgggga
    37441 agaagtcgtt tgtatgggaa aaggactagc ttttcaaaaa aagattgggg acgagatttc
    37501 gccagaagcg gtccaaaaag aatttgtttt aagagattct tttgctaaaa accaattcca
    37561 gcagctgatg gctgatattc cggcagagga gatcgaatgg gtcaaacaag tagtcgaact
    37621 ggctgaagat aaactaaaag ttgaattttc gcctaatatt tatctgacat tgaccgatca
    37681 tcttcattat gcgatcacac gagcaaaaga aaatatccaa ctgcctaatc cattattatt
    37741 tgaaacaaaa aaattttatc cgaaagaata tcaagcagcg aaagaggctc ttttgttcgt
    37801 gaaacaaaag acgggtgtgg aactttctga ggatgaagcc ggttttattg cctttcattt
    37861 tgtgaatagc caacaaggaa acgaagacat gcaagtgacg atgaccgcta ccacaatggt
    37921 aagagatata ctgagtatca tcagtaaatt cttcggcatc gtgttcgatg aagaatcttt
    37981 gaattatcag cgtatcatta ctcatctgca gttttttgcc cagcgttatc tcaaagggga
    38041 aggatctgac gagcaagatg aatttctcta cgcattagtt caaggaaagt atccaaaagc
    38101 gtttcattgt gtcgaacgga tcaatgagta tcttcgacaa acgcaaaaac aggaaatggg
    38161 gatcgcagag cagatttatt taacgattca tattcaaaga gtagtaagtg agaaaaaaag
    38221 catccaatca taaggattgt tactgtatgg caggcaaaac ctggattgaa aaaataggtt
    38281 cacgaatgtt tgtgactgtt tttttcaatc tgggttttta ttttttaaat aaaaaagaaa
    38341 gaaggaaaaa acaatggata atcaagcagt tggtcgtcgt gtatgggaag cggtaggagg
    38401 acaaaagaat gtcaaaagtt tagtacattg tgctacgcgg ttgcgattca gattaaaaga
    38461 tgaatcttta gcagatacgc aaaagttaaa agaagacccg gacgtgatcc aagtcgtaca
    38521 aagtggcggt cagtatcaag tagtgattgg cagtaatgta gcagatgttt atcaatcgat
    38581 cgtggatgag gaaggtcttg cagtttcgga ggatggaaaa gaagagtcca aaaatatttt
    38641 gaatcgtctg atcgatatta tctctagtat atttacacct tttctagggg cgatggcagc
    38701 cgccggggta ttgaaaggat tcttatcatt ggcaacggtg atgggctggc tgacagctga
    38761 tagcggtact tatcaaatat tgtttgctgc agcagatggt gtctttacct tcttacctgt
    38821 catgcttgct tttacagcag ctaaaaagtt caaagccaac cagtttttag cagtagcaat
    38881 tgctatggca cttgtgtatc cagctatcac agcagttgct ggtgcgggag aagctatcag
    38941 tttctttggg atcccagtga ttctttcacc atctggttat acttcttctg tcataccaat
    39001 cattctagcc gtatgggttc aaagtaaatt agagccattc gtaaaaaaag tgattccgca
    39061 atttctgcaa atgattttag ttccgcttgt cgttttagta gtgatggttc cattaacatt
    39121 tcttgcttta ggaccaatcg ggacggttgc cggtaatgct ttaggcggtc tcttcaactc
    39181 gatttatgga tttagtccaa tcgtagcagg tctaatcatg ggaagtttat ggcaagtatt
    39241 cgtgatgttc ggcatgcatt ggggattcgt accgatcatg tttttgaata tcgaacaata
    39301 tgggtttgat gtattgatgc caatgcttct tccagccatc ttggcacaag gtggggctgc
    39361 tttagctgta gcattgcgaa caaaagatac gaaacttcga gcattaggga tttcttctac
    39421 agtgacttcg ttatttggaa tcactgaacc aactgtgtat ggtgtgacat tacctttgaa
    39481 gaaaccattc attgccgctt gtatttctgg aggtattggt ggagctatta tcggattttc
    39541 cggagtaaaa gctttctcca gcagtttggt cagcttattg acgattccga ctttcattaa
    39601 tacggtggat ggtgtagagt caaatgtgac agtagcagta atagctacag gaattgcttt
    39661 tgttcttgca ttcgtaggta cattgatttt aggatttgac gaacaaacac aagacaatca
    39721 attagaaaac aaacatgcga atgcaggaga accgattact tcagcacggc ataccttaaa
    39781 aagtccttta acgggtaaag tactgccatt gagtgaagta ccagaccaag ttttttcttc
    39841 tggcgtgatg ggaaaaggta tcgctattga tccagaagta ggagaactag ttgcacctgc
    39901 agacggagag atcacgacta tttttccaac tggacatgct gtagggatca caacgactga
    39961 cggagcggag attttgattc atatcgggat ggatacagta gaattaaatg ggaatggctt
    40021 tgagatctta gtcaaacaag gggatctcgt caaagcagga gatttattga ttcgttttga
    40081 catcgaagca attagagcag ctggctatag cgtaatcact ccagttgtca ttacgaatac
    40141 tgatgcattt gccgatatac ttgaattaga ccaaaaagag attattgcaa acgaagatgt
    40201 tctggcaatt gtaaaataag gaggaaatca ggatgcaatc acgtttttca gaaaaatttt
    40261 tatggggtgg agcaactgct gccaaatcaa ttagaagggg cttatgatcg agacggaaaa
    40321 ggactatccg ttgcagatgc tatgcctgga ggtaagcagc gctttgcgat tatcggcagt
    40381 gaagaatttg actggacgat tgatcaagaa aaatatattt acccgaatca tcgaggaatc
    40441 gatcattacg atcgtttcaa agaagatctt gccttgtttg cggaaatggg attcaaatgt
    40501 tatcgttttt cgattgcctg gacacgaatt tttccaaatg gagacgaagg tactcctaac
    40561 gaagcgggat tagaatttta tgatcagctg attgatgagt gcttaaaata tgatattgaa
    40621 cctgtgatca cgatttctca ttacgaaatg cctcttcatt tggcaaaaga atacggtggc
    40681 tggaaaaatc gaaaactgat cgacttctat gaacgatttg ctcagacagt attagagaga
    40741 tacagcagta aagtcaaata ttggatgact ttcaatgaaa tcaattcggc ctttcacttt
    40801 ccagctttaa gtcaaggatt agtaaaaagc aatggagcag gagagtacca aaatattttc
    40861 caagcatggc ataatcaatt cgttgcaagt agcaaagccg taaaaatcgg ccacgaattg
    40921 cgttcggata tccaaatcgg ctgcatgatt atctatgcaa caacttacag catcgatgcc
    40981 aatccagtca atcaggcagc aacaatgatc caaaaccaag aattcaattt tttctgcacg
    41041 gatgtccaag tacgtggaga ataccctgca tacacagcgc gtacccataa aaaatatgga
    41101 gtcgatccgg aaaaattaga gcaaacggaa aaagacttcc agttgctaaa agaatatcca
    41161 gtggactaca tcggatttag ttattatatg tctacggcaa tcaatgagac tgatccacaa
    41221 gcagcaactt cagaagggaa cttgctggga ggagtaaaaa atccgtttct tgaggcaagt
    41281 gaatggggct ggcagatcga tccagaaggg ctgagaatcg cattgaatga attatacaat
    41341 cgttaccaga aaccattatt cattgttgaa aacggtcttg gagccattga tcatatggaa
    41401 gaggatggca cgattattga tgattatcgg atcgattatt tacgtcgtca tatcgaagcg
    41461 atggctaatg cagtagcaga cggtgtagat ttgatgggct atacgccttg gggatgtatc
    41521 gatcttgtca gtgcatcaac aggagaaatg agtaaacgtt acggctttat ctatgtggat
    41581 ctagacgatg aaggaaatgg gacactgaat cgatcaaaga aaaaatcttt tgattggtat
    41641 aaagaagtaa ttgcctcaaa tggcgtaaac ttgtaattaa agcagctgaa tgaaaaagaa
    41701 cacacttctc acatgtgatt tgtgttcttt ttttattttt tatgtgctat tcttttagta
    41761 atagtaaagc aaagggtgac gttatgaaaa ggacacgtgt actatacttg gaagtcgcag
    41821 ataaaattaa agaagatatt ttctcaggaa aatatccagt cggttcgatg ttgccgacag
    41881 aaagtgaatt agaagaactg tttcaagtca gcaaaatcac tgtccgaaag gcaatcgaac
    41941 tgctggcatc agatgaatat gtcgcaaaga aaagtggtcg tgggacgacg gttctaagca
    42001 atcgtcctta caatcgatta tccaaagcgg caagctttac gcaaatcttg gagaactccc
    42061 gatatgaagt ccggaaagaa acgctggatt tgcaattggt ccatataaag aaggaagatc
    42121 ctttgttttc ctttttcggt gagcaagctg tttgttttaa gcgcttatat tatttgaaca
    42181 atcagcctta tatttatttt gttcattatc ttccacaaga aatgatgcaa ttcagcagag
    42241 aagaatttgg caaagaatct ttgtatcgtt tgctgacgaa aaaaggatat actattgact
    42301 cattttctga tgatttctcg gcagtatttt taacagaaga agaacaagga attctgaaaa
    42361 cagacgcaca ga
//
LOCUS       DAMEET010000010        42200 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.010, whole genome shotgun sequence.
ACCESSION   DAMEET010000010 DAMEET010000000
VERSION     DAMEET010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 42200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.010"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            159..1022
                     /locus_tag="PB780_000494"
     CDS             159..1022
                     /locus_tag="PB780_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDE0732569.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1151..2773)
                     /locus_tag="PB780_000495"
     CDS             complement(1151..2773)
                     /locus_tag="PB780_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDE0732570.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3022..3543
                     /locus_tag="PB780_000496"
     CDS             3022..3543
                     /locus_tag="PB780_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDE0732571.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3729..5459)
                     /gene="spxB"
                     /locus_tag="PB780_000497"
     CDS             complement(3729..5459)
                     /gene="spxB"
                     /locus_tag="PB780_000497"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HDE0732572.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5624..6442
                     /locus_tag="PB780_000498"
     CDS             5624..6442
                     /locus_tag="PB780_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDE0732573.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6545..6742)
                     /locus_tag="PB780_000499"
     CDS             complement(6545..6742)
                     /locus_tag="PB780_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732574.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6911..7489
                     /locus_tag="PB780_000500"
     CDS             6911..7489
                     /locus_tag="PB780_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HDE0732575.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7584..7721
                     /locus_tag="PB780_000501"
     CDS             7584..7721
                     /locus_tag="PB780_000501"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732576.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8534..9037)
                     /locus_tag="PB780_000502"
     CDS             complement(8534..9037)
                     /locus_tag="PB780_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDE0732577.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9396..10421
                     /locus_tag="PB780_000503"
     CDS             9396..10421
                     /locus_tag="PB780_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDE0732578.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10768..11646
                     /locus_tag="PB780_000504"
     CDS             10768..11646
                     /locus_tag="PB780_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HDE0732579.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12122..12238
                     /locus_tag="PB780_000505"
     CDS             12122..12238
                     /locus_tag="PB780_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDE0732580.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13166..13504
                     /locus_tag="PB780_000506"
     CDS             13166..13504
                     /locus_tag="PB780_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732581.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13598..13828
                     /locus_tag="PB780_000507"
     CDS             13598..13828
                     /locus_tag="PB780_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732582.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14228..14599
                     /locus_tag="PB780_000508"
     CDS             14228..14599
                     /locus_tag="PB780_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDE0732583.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(14873..15565)
                     /locus_tag="PB780_000509"
     CDS             complement(14873..15565)
                     /locus_tag="PB780_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDE0732584.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15568..16353)
                     /locus_tag="PB780_000510"
     CDS             complement(15568..16353)
                     /locus_tag="PB780_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDE0732585.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16590..17990
                     /gene="lacG"
                     /locus_tag="PB780_000511"
     CDS             16590..17990
                     /gene="lacG"
                     /locus_tag="PB780_000511"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HDE0732586.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            18015..18332
                     /locus_tag="PB780_000512"
     CDS             18015..18332
                     /locus_tag="PB780_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDE0732587.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18345..20018
                     /locus_tag="PB780_000513"
     CDS             18345..20018
                     /locus_tag="PB780_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HDE0732588.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            20032..21663
                     /locus_tag="PB780_000514"
     CDS             20032..21663
                     /locus_tag="PB780_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HDE0732589.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            22535..23725
                     /locus_tag="PB780_000515"
     CDS             22535..23725
                     /locus_tag="PB780_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HDE0732590.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            23859..26048
                     /locus_tag="PB780_000516"
     CDS             23859..26048
                     /locus_tag="PB780_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDE0732591.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            26045..27838
                     /locus_tag="PB780_000517"
     CDS             26045..27838
                     /locus_tag="PB780_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDE0732592.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLY
                     KFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGI
                     TLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNL
                     FLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            28038..28352
                     /locus_tag="PB780_000518"
     CDS             28038..28352
                     /locus_tag="PB780_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDE0732593.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            28373..28750
                     /locus_tag="PB780_000519"
     CDS             28373..28750
                     /locus_tag="PB780_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDE0732594.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            28760..29533
                     /locus_tag="PB780_000520"
     CDS             28760..29533
                     /locus_tag="PB780_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732595.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            29555..30958
                     /locus_tag="PB780_000521"
     CDS             29555..30958
                     /locus_tag="PB780_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDE0732596.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            31140..32324
                     /locus_tag="PB780_000522"
     CDS             31140..32324
                     /locus_tag="PB780_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDE0732597.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            32321..32611
                     /locus_tag="PB780_000523"
     CDS             32321..32611
                     /locus_tag="PB780_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732598.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            32608..32829
                     /locus_tag="PB780_000524"
     CDS             32608..32829
                     /locus_tag="PB780_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732599.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            32871..33650
                     /locus_tag="PB780_000525"
     CDS             32871..33650
                     /locus_tag="PB780_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HDE0732600.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            33717..34211
                     /locus_tag="PB780_000526"
     CDS             33717..34211
                     /locus_tag="PB780_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDE0732601.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            34267..34905
                     /locus_tag="PB780_000527"
     CDS             34267..34905
                     /locus_tag="PB780_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732602.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            34977..35372
                     /locus_tag="PB780_000528"
     CDS             34977..35372
                     /locus_tag="PB780_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDE0732603.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            35356..37809
                     /locus_tag="PB780_000529"
     CDS             35356..37809
                     /locus_tag="PB780_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDE0732604.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            37806..39818
                     /locus_tag="PB780_000530"
     CDS             37806..39818
                     /locus_tag="PB780_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732605.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETKY
                     SKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSNATGYL
                     VKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYVAYTGL
                     VKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTKIVLPN
                     TDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDTNNGED
                     RENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFSQVLFI
                     IFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTMLYSLSA
                     SSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNRQTRRF
                     QRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQQGGIG
                     QRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDFKQSMA
                     DTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRKRQQTV
                     TPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            39815..40837
                     /locus_tag="PB780_000531"
     CDS             39815..40837
                     /locus_tag="PB780_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDE0732606.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            40854..41783
                     /locus_tag="PB780_000532"
     CDS             40854..41783
                     /locus_tag="PB780_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDE0732607.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            42028..42144
                     /locus_tag="PB780_000533"
     CDS             42028..42144
                     /locus_tag="PB780_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HDE0732608.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT        13818 a         6976 c         8338 g        13068 t
ORIGIN      
        1 cgtaaattcc ttcttatttc ttgaagctga ccacttctgt cacaacctct tattttgatt
       61 agaacttgcg aaaataaacc ggttaaagtg tgtctagcgc aagagatttg ctataataga
      121 cgataagggt aaaatgtaac gagaaggggt tgaaaaatgt gtatcaaatt gtaacagatt
      181 cttgttgtga tctgccatac catgtattag caaaagaaaa agttgatttt ttatcaatga
      241 atctccaatt gaatgatcaa gagttagtag atgacctcgg aaagacattc aattatgacc
      301 gctacatgaa tcagctgaaa aatggtgcga tgcctacgac atcacaaatc aatgttggcc
      361 gatatttaga atttttccgt tcttatgcgg aaaaacagat tcctgtttta tatgtggctt
      421 tttccagtgg attaagcggc tcttacagta gtgcgcttca agcggttgaa ttgctgaagg
      481 aagaatatga agaccctgag atctatgttt ttgatacaaa agctgcaagt attggggaag
      541 ggcttttagt attggaagct gtaagattga aaaaagaagg taaaagcatc gaagaaaccc
      601 tagagtggct ggaaagtaat tatttgaaag tccattcatg ggtaacagta gacgatctga
      661 aatatctaga acgaggcggt aggatttcca aagcaagtgc tactcttggc agtctattga
      721 gtgtaaagcc tattattacg gtggataaag aaggaaggct gacgaatgtt gacaaagttc
      781 gaggaagaaa caagtcgatc cagacaatcg tcaatgagac ggtaaaaaca atcgttgcac
      841 cacttgatca aatcgtttat gtggcttacg ctggggatct agagagcgcg gagaaggcaa
      901 aacggctttt ggaagagcag ataaagatga aagatgtgaa gctttatccc ttaggaccaa
      961 cgatcgccag tcacacaggc tatggctgta tcgcgatttt ttcgatggga atcagtcgat
     1021 aaaagcagta actcattctg taaaaaagcc cagaaattga ttgtaacaaa aacagcgact
     1081 cttaaaagat agatgagcat cctatctctt agaggtcgct gtttttttat tttttaagct
     1141 aaataggcca ttatgcccat aactcgttca attgtttttg aatatctgca ttttccaaaa
     1201 attcatcata tgtcgtatca gctcgatcga tcacaccttt atccgaaacg gcaatgatgc
     1261 ggtttgccaa tgtttggata aattgatggt catgtgacgc aaataagatt gagccagtaa
     1321 atgccatcaa gccgtcattc aatgctgtga ttgattctaa gtctaaatgg ttcgttggat
     1381 catctaatac aagtacgtta gctttcgaaa gcatcatttt tgaaagcatg caacggactt
     1441 tctctccacc agataaaaca ttcactggtt tcaatacttc ttcaccagaa aacaacattc
     1501 ttcctaagaa gcttcttaag aaggtgttat catcttcttc tttacttgca tattgacgca
     1561 accagtctaa aatcgttaga tcagtatcga attctttcgt tgtatccttt ggtaaataag
     1621 aattacttgt tgtcacaccc caacgtactg ttccagtatc tggcgtaatt tcacccatga
     1681 taactttgaa tagcgttgtt gttgttatat cattttctgc gacgaaagcg accttgtctt
     1741 ctttgctcaa attaaaagat atatcatcta aaattttctt tccatcaatc gttacactta
     1801 cattttctac ctgcaataag tcgtttccaa tctcacgttc aggtttgaat ccaacgaatg
     1861 gataacgtct agaagaaggt tggatatcgt ctaaagtaat tttttcaagc atttttttac
     1921 gtgaagttgc ttgttttgat tttgatgcat tcgcactaaa gcgggcaata aattcttgga
     1981 gttctttgat ttgttcttct tttttggcat ttgcgtttgc ttgaagttta gccgctagtt
     2041 gactagattc caaccaaaaa tcataatttc ctacataaag tttgatttta ccaaaatcta
     2101 gatccgccat atgtgtacat actttgttca aaaagtgacg gtcatgggat acaacgatca
     2161 cagtattttc aaaattgatc aaaaattctt ccagccaatt gatcgattgg atatccagac
     2221 cgtttgttgg ttcgtctagc aaaagaacat caggtttacc aaatagtgtt tgtgctaata
     2281 acacttttac cttttgtcca ccagttaatt cactcatttg ctgatggtgc aattcttcag
     2341 ggatgttcag accttgcagt aaaacagctg cttctggttc agcttcccat ccattaagct
     2401 cagcaaattc tccttctaat tcagctgctc gaataccatc ttcgtcagtg aaatcctctt
     2461 tcatgtaaat cgcatctttt tctttcagca cttcatacag acgcttgtgt cccatgatga
     2521 ctgtttcaag tacagtatag tcttcatagt cgtaatggtt ctgtttcaat acggccatcc
     2581 gttcatctgg tcccatagta acggaacctg tggaaggctg cagatcccct gataacactt
     2641 taaggaacgt tgatttacct gcaccatttg caccaatcaa gccatagcag tttccggggg
     2701 taaatttaat attcacatca tcaaatagtt tgcggtctga aaagtgcaaa ctcacatcat
     2761 ttacagtaat caattttttt cctcactttt atcattatta gttttgacaa gtcttataga
     2821 attatatagg ggaaaggtaa aagattcaag gcaatcgaac attctttgtg tttcacgtga
     2881 aacagaatga aaaaaataaa aataaaagcg gagcgaaaat agctactata ggatttatta
     2941 gtttgcaaca gcataatttt tgacgttatt tataagaggg agtactataa aactgtaaaa
     3001 gaaatgaaag aaggattgaa aatggatgaa aaaagaacca tcgactgggg ctcattgatt
     3061 ttgggaattt tatttgtact tgtctcattg ttatcatttc aagaccctgt cggaaattta
     3121 gttgcaattg tagtagtctt tgcgattttt gcctttatta aagggatatt tgaactgttt
     3181 gtacggaaca gattaaaaga gctaacagga tataaaggaa agatgccctt aataatcggg
     3241 atcatcgata ttctagttgg cgtctttttc ttattcaata ttggtgcagg agtcgcagca
     3301 ttgccttttg tttttgcagt atggtttatt gctgattcag ttttagcgtt atttactgct
     3361 gacttggcaa gaggagtaag tgaaggatat tattggttta cgatcatcgt caatatttta
     3421 ggaattttat taggaatttt cttattgttt aatccaattt cttctgcgtt gacattaagc
     3481 tttttagttg gattctattt tatgttattt ggtatcacac atatcgtcta tgcatttaga
     3541 taagtaaaat caaacaaata aaaaagccgg cttccgatta cagttttact gttgtaattg
     3601 gaagccggct tttttatcat ggacaaatat gcacgaggtg atttttaggt gtcgagacaa
     3661 atttatgcaa aaatgtgaga agctatatac acaaatttta tcttaatact cgaagcatag
     3721 cgcagttgtc acaacctctt ttcatataat tcattcaata ctggcaaatc atggacttca
     3781 tatttttctt tgaatgcttg gatttcttct tcagaatatt ttcttggatc taaatgcaga
     3841 tcttctactg gcaatgggcg cttgttattg atcttcacat cgattactac aggacgatct
     3901 gattttgcag ctgcatcaaa ggcaaatcga agttgcgagt attctgtgac tgtaaagcta
     3961 tttgccccga gcgcttctcc aactttaccg aaatctgcat cttctaaaaa tacaccaaat
     4021 ttcttttgtt ccgtatcttc ttgttctgct tcgatgaaac caaaagaatc attcgaaaag
     4081 acaacattaa taattggaag ctgatatttg acttgtgtaa tgatatcttg catgaccata
     4141 gcaaacccgc catcaccact taaggtaaat acttgttttt ctgggtagct tagttgtgcg
     4201 gcaattcctc ctggcacacc attacccata gttgcgaacc atccagatgt cgtatgtttt
     4261 tgtttaccgt tcatattcaa taaacgaata gaatggatag tcgtgttacc gacatctgtg
     4321 acgaaaatcg catcgtcttc agcaatacga ttgatttctt tatagacagc ctctggacga
     4381 atcggttctt cctctcgatc ttcaaaagat ttcaaccaat ttacccattt ttctttattt
     4441 tcttgatttg ctaggtacca gctatcagca gggcgagcat cccctaactc gacaagtctt
     4501 tgcaaagctg tgtttgcatc accgaggatc gacaaagatg tttcatggcg tcgtccaaat
     4561 tttgacgcat cgatatcgat ttggataaat tcagcagttg gattaaaaaa tgcacgtcca
     4621 aatgggaagt cgctaccgac aaataagatc agatcggctt ctgccaatgc ttcatttccg
     4681 ggtttagtag cgacacgtcc agcaaaacca agaaagtttt cataatcatc aggaataatt
     4741 ccttttgcta gaacggaagc gacaacagga actgaaaaat gttctgagaa ttctttgatt
     4801 tgcggaaatc cgtttcgcgt tccttgacca acataaagaa ctggtttttt tgctttttca
     4861 agataaggca aagcagctag caaatctttt tcttctggta atatcacact tgttttgtga
     4921 gtgtgagcgg tggagaaagg ttgttcgtca atttcttcaa aaccaagatc aacagggata
     4981 gtcacgatag ccacgccttt atgctcatag gcggctttaa tggcttcatc tactacatgc
     5041 ggaagacttt gtggtgtcat gactgtacga ttgtatacgc taacatctgc gaaaatagga
     5101 ttttcattta gttcctggaa agagttatag ttcatggatg tagaagcaac ttgtcctaag
     5161 aacgctacta gaggcacatg gtccatctgt gcatcataca aaccattgat caaatgtgtc
     5221 gcaccaggtc ctgcagagcc gaagacagcg ccgactttcc ccgttaattt tgcatctgca
     5281 gcggctgcga gtgcgccgac ttcttcatga cgaacttgaa tgtagttgat ttcggttttt
     5341 tcttgatata gcgcatccat aattgaattg aaagatcctc cgggaatgcc gtaaatatgg
     5401 tcgatacccc agctttcaag aacttttacc attgcgattc ctgcattgat ttttgacatg
     5461 gtgtttcact cctttagcaa tgatgataca taaatttttt gtatctagag tatagaactt
     5521 tttatcgaaa atcccaacta attcgtcttt tcacaaaaat gtagtcatgt ttttgggaat
     5581 atgctagagt agtacataga tagattcaag agaggggaat ggcatggttg aatggctatt
     5641 gagacttgaa ccttggcagc aagcacttgc tgggacagct tttacttatt ttatgacagc
     5701 tcttggtgcc gggttggttt ttttctttaa agaaatcaaa aaagaagtgc taaacttgat
     5761 gttaggattt gcttctggtg tcatgattgc tgcaagtttt tggtctttgt tagatccggc
     5821 tattacaaaa gcagaagaaa atggagatat tgcttggcta gttgtaagta ttgggtttgg
     5881 tttaggcggt gtgtttttat atatggcaga taaaacactg ccccatatgc attttggacc
     5941 acaacatgaa gcagaaggat tgcctaccca tttaaaacgg acgatcttgc ttgttttttc
     6001 tattacattg cataacattc ctgaaggttt agccgtgggg gtagcattcg gtgcagcagc
     6061 aactgcggat aatccgacag ccgctatatt agccgctgtt tcggtggcgc taggtattgg
     6121 tatccaaaac ttcccagaag gagcagctgt atcaatcccg ttgcgacaag aaggtctgag
     6181 tcgtaaaaag gcctttgttt acggacaagc ttctggaatc gttgaaccaa ttgctggagt
     6241 gattggagcg ctactagtaa cgaaagtgga gctgctgctt ccttatgcac ttgcttttgc
     6301 cgcaggagca atgatttatg tcgtggtgga agaattgatc cctgaagcac agcagacatt
     6361 gtccagcaaa cgtcattttg cagtttttgg cgtcatgagt ggatttatca ttatgatgat
     6421 tttggatgtg gcattaggat aaataaaaaa aatatatatg atatatcaaa tagggccgcg
     6481 aacttatatc gcgaccctat ttttttagta agttatatca taatgaagtg aatggtatca
     6541 tacattaata atagtttttt ctatgtgtat ttcctttgat ccagcgaaca agccagatga
     6601 taagtgcgat tgggattaga actttgaccg caaaccataa aagcccgctt aggacactga
     6661 caaaaagact gatgacgaag ctagctgcaa ttagcactaa aagaaccatg aagatattta
     6721 agaacatatt tttttgacgc atacaaaacc ctcctcttgt tcattcttta ctatagaaaa
     6781 agtataacag ggattgttta aaaacacctc caactgaaga ctgattttta cttcatctac
     6841 ttaaatgatc aaacaattgc tttttctctg gtttatcatc taaaatagag cggaaaagga
     6901 ggagaatatc atgcctaaaa atcgtcttga agcatttaca gatgctgtaa tcgcgattat
     6961 catgactatt ttagttttag aacttcatga acctaacgga gacacgtggg aggcgctcgc
     7021 tgcattaggt taccgatttt ttatctatat tgtcagtttt atcattttag cgatttattg
     7081 gaacaatcat catcatttat ttcaagttgt acataagata aatggacggg tcttatgggc
     7141 gaacagtttc tttgtttttg cgctttcgct ttttccgttt gcaacttcat ggatcagtga
     7201 gtacatcaat agcctggctc cagaaatgac ttatggtttt gttaccttga ccgctaatat
     7261 tgcgtattat atcttggcaa aagaacttgt caaagcaaat aaaggccacg agaaagcatt
     7321 gaaggcgatg ttgaataatt acaagaaatc gtatctatcg attggtttga atgtgttagg
     7381 aattgtctta gggtatttag tagcgccagt agccgtgctg atcgcaaaca tcgtgattct
     7441 agctgcttgg atcatcccaa gtcgtaccat cgaatcacaa tatcggtaaa atgatccgtg
     7501 tttaaaattt aagagaaaaa tatagaaaaa ataaataaac gatcaaatat ccgagtgata
     7561 ctagtattca aagagggatg cagatggatc aaaaaagaat tcatgtagga ctgattacta
     7621 gttttgtcac gatgcttatt tatttggatc aaggtcattt gacttggaca gtagttggtg
     7681 tcgtaggatt tttgacatta ttcacagcaa taaaaaaata aacatcttta tgtaatctga
     7741 tgttctatct atttaagaaa agcatgttta cagcagtatg attctgttgt aaacgtgttt
     7801 ttatttgtgg aaaatttaat ttttagaaaa gtgtatcgaa aaatagatag ttagtttttc
     7861 cacagtggat aacttcgtaa gagataaaaa taaaaaatta gtcaaagaag ggcgtataga
     7921 tttcttttga taggagatat acaacagaaa tgaataaata ttaacgtgtt ttcaaacagt
     7981 ataaaacggt gtggtttttg gaataaaaaa ataacatatc tcattttacg cttttgttat
     8041 tttatactta aaaaataacg acacagaaaa taccgtgtct cagatttctc tactacttct
     8101 aaaaactact gtttttttcc tttgaaatgc cttcaaaaag taggatcagg cagaatatac
     8161 tggcagaatc aaggagaagg gagcctaaat aaggagaaaa accatagaaa atataaagta
     8221 attacttata aaacaagctt tttatagaaa aataatatac ctgagtaggg tataaattaa
     8281 gacggaattc ctttctagat tgagatattg actaggaaga atgttaagat tttaagcctt
     8341 taagctaaaa aaaaaccctt tttgttaaat aaggggaata tagcctgtca gtttcaaact
     8401 gcaagtcgtc ctgctgcaat aatatagtag attagagatg aaaatagatt tttttaaacc
     8461 aatgactgaa taaaaagacc gtcatagata cacattggaa tgtgtctatt acggtctttt
     8521 tttacgaacg attttattga agacggacag ttcgggtcaa aaataaaaaa gctacgatca
     8581 gaaataagaa taaaatgtat cccaaaataa tggctagacg gtctaactta tttgtcgata
     8641 gatgtttgtt tagaagttga taggcccata aacctatcag tcctcctgtt gtattagtta
     8701 tcaaatcagt gatgtccgct acgccaatac cgaataggaa ttgaatcaat tctgccaaga
     8761 agcttacaag gaaaataatc cctaattttt tagaaaaaga aagtctcttg aaattgacat
     8821 tcaaaagcac cccaaaagga aaaaagacaa tcaagttata agtggtctca cgtaataccg
     8881 tcgtattttc aaaagaaaaa gtagaaaaag ggacgagatt gacattgctg taagaataag
     8941 tgagaaattg tggaagatta aatgatagct tgaataaaat caaccataat agaaagaata
     9001 aataaatccc taataaaaat tttgatacat accgcataga catctcctcc atttaaaaag
     9061 tataccatcg aaaaccgctg aaaaatcata aaaaagtatg aagtttaagg accaacgtag
     9121 ttatcgtacg aaaattagaa gctttttttt gttgggaatc cggtttcttt aacaaagcta
     9181 agatagcgag ttgcttggaa aagttgctat cttttttctg tcaaaacgac tcggaatgtt
     9241 cgttttttgt tctaatttct ccaagttgtt tactttttga tactaagttt atgaaaaatc
     9301 aagaaatagt tgttatacta ggtaatgctg acaaacggga ttggcatttc atcttggttc
     9361 agcttttttt ccaattacta ggaaggaggg atagattgtt caagacttat tttcgtttca
     9421 ataaaataca agataatcct tttcgtatca tgaatctgat gctacttatc tttattgtat
     9481 tatggttagg ttacataaat catgattttt tgatttcaag tttttcatca ttagggatct
     9541 ttactttttt ctattatcaa aacataccga tgaaatattt gctgaaacgt atgtttctga
     9601 tcggttgcgg actgttggtt gctttcgttt tggggatctt atcgacttat gttttatggc
     9661 ttgaaccatt tgccgtagct ttggttgctt tttccagcag attcgtgttg cgattattcc
     9721 atatttctaa accaggtggc ttgttttttg cgatgctttc tgccatggga acgagcatgc
     9781 agctgccaat cgctcaatta ccgatcgtct cactttattt ttttatgggt gtcgtttttg
     9841 ccctgattgc ggcagtgatt acaaaattac tggattcacg gcctgagcag acaattgaaa
     9901 aagcaacttt aaaagaaaga tttcacgaag agcctttagt gatcattgat tctgttttct
     9961 acagcgctgc cttatttctt tccgtttatg tcagtcacgg cttgaatctg cataatcctt
    10021 attggttgac cttgtcatgt gcatcgattt tgctagcaga aaacttggat gcgatgaagc
    10081 atcgacaagt ccaatatctg attggatcga tgggtgggtt atgtgtgtcg gcattcttgt
    10141 catttgtgcc gtttacgcag ttgcagacca tttttctgat cacttttcta tatggaatcg
    10201 cacaatttct agttgctaga aattatgcag ttgctaatat ttttcttaat ccgatggcgt
    10261 tgatgctatc tacacttatt cgtggagcat acttgatttc tttgatcgaa tatcgttttt
    10321 tagggatcgt cataggaagt ttcatcggtt taggtgttgc gtgggtaatg acggttggtt
    10381 tgcaacatta tttaacggtt gttcgtcaaa aattagaata gaaggacaaa tatatgtgcc
    10441 aaaagaggtt atgacaagat ttttgtcaca acctctaatt caaggtgttc aaaagccaga
    10501 ttctcagcat taagtcaaat tcacacaaaa atgtggaaag gaactttcaa aaaaatttta
    10561 tatttattct atcaaaaaat tttatattta ttctatcaaa aaattttgtt tatgatggta
    10621 actgaataaa ctttcctgag atggtcaatg ctttccttga aagtcttaca gatattcttt
    10681 cttcatagaa agtttaagtt ttatagttag tgtacaataa aagtatgttt ataccatatg
    10741 gtgtccggat aatcagtcat ttaaacaatg aggttcaaag atatgaaaag gggaaatttg
    10801 atgaagaaaa taagcaatga aatccaaatt ctggcagaaa ctttaactct tcccaaagtc
    10861 gttatagagc aaatgattgt acaagcaaaa aacatccttg atgaggtgtt tgagcaatat
    10921 agtcatgaac taatggatcc taataaagca aaggcgactg caaagaaaat agacaaagtc
    10981 tatgaagagg aaccttttgc agcattagct atttatctat atgcagcttc tcaaagttgg
    11041 aaaaaaattt atcaaccatt gaagattcca agaactatct atttggcaac aatgaatgct
    11101 tttactagat ttattcatga acatttccaa gtaacaggaa gctatcgctt tgaccgtggt
    11161 ttttggattt ggcattacac tagtggcttg atctttcgga ttggtgaatt ggagtacgaa
    11221 atgacatatt ttccgaaagg gtacacggat ctaagattag aaggaaagca gtgcctttct
    11281 cttcatattc cttccgatgc agatttatct cagaataaga taagagatag ttacgaaagt
    11341 gccattcgtt tttttgaact ttattttcct gattaccatt accaagtgat gtacacagat
    11401 acttggttac tatcacccaa tcttactaaa tggttgaaaa aagagtcaaa aatctgtctt
    11461 tttgcagccg attatcgatt attatcagtt gatgaacagg acgatagtgg agtcccttgg
    11521 atctttggac gagtagatgc ccaaatacat gattatccag aaagtacgag tttacagcga
    11581 caagcaaaag agcaattgct tgcaggcgag cacattggct ctggcttagg tattcttact
    11641 tcataagttt agtacttcga aatagtttaa aagagcgtga gacaaaagta aagaacactt
    11701 ttgtctcacg ctctaaacac gtataaacgg cggaagcaga agcaactcct tcggaaataa
    11761 gctgaaattc acaaaaattt gaaaagcaat tttcgtgaat ttcttcttat ttcttggagt
    11821 taaacgcttc tgtcccagcc tcttttttag tgttctatcg aacacacagt acgttcaata
    11881 ggtaaactag ctataacaat agatattaaa gttaacggac aagagggcct tttttaggta
    11941 gctaaaacgt aataatgaaa atttatttcc agagatagga gtcaatcagc tagtctgatc
    12001 gactgtttta acaagttgac tttcatttta acttttgata ccatttaaaa cacaaatcgt
    12061 aattgtttcg atttgtgttt tttagcatga ctacaatcaa agaagaaagt gggtcgtcca
    12121 tatggaaaaa acagatatct caagtgcata ccgccgcttg aaaagcccaa atatcaaaac
    12181 acgaaaacgt gcgctgaaga tcatcaaaga tgtgaaacgg aatagtggga aaaggtaaga
    12241 agaaggaaac ttaattaaaa aaatagccga aaaacgtttt tcggctattt tcaataatcg
    12301 gtttttgtgt aaggtttccg aaagaactgc tgttgatttt ccatttttaa catatgataa
    12361 attggtagaa tctttctttt caaattgata ccattcccta attccagtta tatagttaac
    12421 tacagaacca caaagcacgt acgtttataa aagtattatc tgtagttatt tttcaattcc
    12481 ctttcaacct cttattttga ccagtagtat tgatcacttt accttttaac acctttgttc
    12541 gtttttgaaa catttttagt caagaatgga aacgtaacta cataatcaaa ggttttgcga
    12601 aatatttcat gaaatatttc atgtagaagc gagcaatacg gttgcaaata accattatcg
    12661 tttaataagc agttacgaaa gctcccaata tctctaacgt tcagctcact ccatttcttt
    12721 tcttcaaaaa aatcagtgat tcgtatctct ttctatttgt cgaaaaagtt aactttaaaa
    12781 agtaacgccg aaaaaatatg caaaaaaata aaaaagcagt tactattgct agaataacat
    12841 caaggaataa taagttttgg ttttcagata atagtcgata agttgtgtat tggaatttta
    12901 gatacagtaa taaagtattt ttcatgaaat tttgtgagaa tcactattac aagtatcttt
    12961 aataaaatta atggttaagg aggaacttaa agtagaatgt aaatgaatag ttgtagtcag
    13021 tcgattgtga tactagactc aaagttgcag gaataagttt ttcaatttgt gattggaaga
    13081 taaaaaatga aaaagaaggg ctttttttta tactttgttg attgtagtgt ttattttccc
    13141 tctgtttagc caagtaacat tttctatgac tcaaccagtt tcaagtgagg aacatgataa
    13201 cctttttcgt gctacagaag agtttaagaa atatcaggaa aatattgttc cggagactcc
    13261 attagctagt gagccaatta ctgatattga tggaaaccaa ataggatggt atatacagta
    13321 cgaagttatg gtggtaaata atgactcaat agtaaaaaat acagtaggct tagagctaac
    13381 accgattgaa aaggtgagtt cattgttaac gtatatttat actgatgaaa gtgacgagac
    13441 tgagatcatt ttaacggatt actcaaaaat tgctactgat aaaaagtata tattgtggat
    13501 gtagacacta gtgaaatact ttatgatata gaaattgatg aaaatttact agatgtagca
    13561 caagaaatcg atcatcaaaa gcaggaagct attcgatatg ctggtagaaa agggaaaaga
    13621 aaatatttat tacgtgaatg tagcaaaagt tagagaagat gaaaatgagt ggaaagaatt
    13681 caagagtcgc tactcaatta actcgactcc gacatttaca gtttacagag aaggatcgat
    13741 agaaaaaact gttttttgga ctaaagaaag tggaatgtct ttagctgaag tagaagagtt
    13801 tttggattat gttagtatgc aacagtaagg gagttcttat tatgaaaaat tttgacacat
    13861 attatgttat tgttttttcg atatctttgc tgttaatatt actaacttta atgcagtata
    13921 aagagtatcc agagtatttt attttaactt ttgtatattt agtaagtagc tacacacttc
    13981 tatataaatt cagaagttct tctagagttt ctaaagtttg tttcttaatt agcttattga
    14041 ttctataata tttttgattt atataggaaa ttaaaaatat tatagaatca acactcagag
    14101 aacgggttgg tttttaatag atttaataag tactgataat gaaatttctc ctggttggga
    14161 aaacgaagtt ggttaccctg tagaagaatt ttttcaagat tactttgcgg agtatgccga
    14221 taggataatg agagattacg taacagataa aactcaagaa gtcatcaatc aatcccagtc
    14281 attaaatgaa ttatctgata aggcgaaaga agaagcagtt gccattattt cttcagggat
    14341 tagtgactat attgatatga gtggtgaaat caattatggc gataaagaca aaattgtacg
    14401 catgataaaa gcttttatca aaacttttga tgtgaaggca gatcttaagt ttaatgatac
    14461 ttacctagta ggaaaattaa ttaatatcct tgggacagag aaagatacgg aaacgtttat
    14521 ttcctctttg tctatgttgt ttacagataa gcatttttca actcagcttg gtggagagga
    14581 actagtggaa atcttttaac cattacgccc agcattaata aaagtatatc aaaaaagtac
    14641 tcctgatgat ttttaagatt ggtttgagaa ataatgtgaa aacactattt ttaatgttaa
    14701 ttatagatag agatgaacgc gtcatatggt taaaagaatg aaaaagtcaa agatacactg
    14761 ctatatttca ctaaggatat agcagtgtat ctttcatgtc gtttcactgg cagtttttta
    14821 taaaaggagg tgtgcatttc agttgctttt ctttggtagt tagttgaatc gtttaagaat
    14881 taacggatgt ggcagacagg tgctctgtcc aaaaaccagt aaaatctttg atggttccat
    14941 ctttatttag ctgtgcctgt tcaaatactg ttttgttttt accgaatgga tcaattgcaa
    15001 taattgttcc gatccccgca cgtttggcag caagtaatcc tgagtaagca tcttcgataa
    15061 caagacaatc agccggagct aagctcagtt tgtctgccgc tttaagaaaa atatccggtt
    15121 gcggtttccc agggaaagag ccgtcgtcat agacaatctt gtcccagtca aaccagcggg
    15181 ctaaatcgaa aatatcaaaa taaaaagcaa cattctcttt gacagtagcc gtggcaatcg
    15241 ttaatggaat ctgttggtca gtcagctggt caagagtcgc ttcaagccct tttgtcaact
    15301 gaagctcgtt ttcattttgc aagcaaagca tacgataata gctttctttt tcttctgaca
    15361 atacggcgat ctcagaggcg gtcaaatgct ctgaaatgaa atgtttcaaa atctccgtat
    15421 tcgtccgacc atgaatattt tttaaaatat cctcgtctga taaaccatct tttgtatgat
    15481 catgtatcat atgcagccaa gcagcttcat gcagatgaga gtccaaaaac attgttccgt
    15541 tgaaatcaaa tataatccct ttcatagtta cgctcctata gtggtatttt cggttgaatc
    15601 agcaatttgc agatcgacat attgtgcata gtgctcttga acctcccgcg agatctggcg
    15661 atccgtaatg agcagatcga aatcatgcaa attataaaac acataaaaat catctcgatt
    15721 gaatttttta ttatcaacga gtagatattt cttacgggaa ttatttagag cgatctgctg
    15781 tgcttctcct tcttcggtac tgtaagtaga gatcgcttcg ttgtgaacgc catttgaact
    15841 gataaatgct tttgtaaact ttagtcttct taaattgtcg ttggtgatcg gtccgacaaa
    15901 tgcgccggta tttttccgga aatcaccgcc aatcagtaga atttctggtc catctgcttg
    15961 agcaagaata tcaaaaactg gataattatt tgtaatgatc cgaattttct tgttcagtaa
    16021 atgcgtcgcc aatagttcga ttgttgtacc tggtcctaag aaaatcgttt ctccctcgtg
    16081 aatcaaggag gctgctgtgg cagcaatttc ttttttttct tgaatctgca gagtggcttt
    16141 ttcagtgtga gaaagttctt gatctaaagt gtaggaaaga ctttgagccc ctccatggac
    16201 acgcagcagc ttgcccgatt tttctaattc atctaaatct ctgcgaacgg tcatatcaga
    16261 aacatctaat tgattgatga tttcattaac ggtaaggatt ccttttttat tgaccatttc
    16321 tacgatagta agcaatcttt cttttttcaa cattgtcttc acctctaatt taaggataat
    16381 ctatcatata aacggacaac agtcaatcta attcgaacaa aagtaaacat ttagggtggt
    16441 ttttatgttt gttacaatag attttattgc tgttttctgg tttttatgat tatttgtgtt
    16501 tgtttttgtt gacaaatgtt ttggatgcgt ttacaatgca atcagttaga aagagcgaaa
    16561 actgattata gatttgagga gagatgtaca tgcttaaatt accagaagat tttatttttg
    16621 gcggagccac agcagcctat caggttgaag gagccactaa ggaaggtggc aaaggcgctg
    16681 ttgcttggga tgacttttta gaggaacaag ggcgattcag tcccgatccg gcaagtgatt
    16741 tttatcatca atatgcaaag gacattgaat tgtgtgaacg ttttggtgta aatggtctaa
    16801 gattatccat tgcttggagc cgcatttttc ctgatggagc tgggaaacca aatccagaag
    16861 gaatcgcttt ttatcatcgt gtgttcgaag aatgtaagaa aagaaacgta acgccgtttg
    16921 tgacgttgca tcattttgat acccctaaac gtttgtttga ccatggagat tttttgaatc
    16981 gagaaacaat agaagcattt gtttcctatg ccattttttg ttttcatgaa ttcaaagaag
    17041 tcaaggtgtg gagtaccttt aatgaaatct atccagttgc aaccaatcaa tatttgttag
    17101 gtgttttccc accaggaatc aaatatgatt ttacaaagat cgtcgcctgt cttcataata
    17161 tgatggttgc tcatgcccgt gtagtgaact atttcaaaga aaacgaactt ccgggtgaaa
    17221 ttggtgtagt tcactcatta gagacgaaat atgcggcaac agacgcgccg gaagataagc
    17281 atgctgcctt tttagacgat gcattgtcga tccgtttctt attagatgct acctatttag
    17341 gctattattc aactgaaacc ttgactgctc ttgatgagat atgtgaagca aatcaggcca
    17401 gctatcattt tcctgaagaa gatttcgttg aattgaaaaa agcatccacg cgtaatgact
    17461 acttagggat caatcactac caatgccatt ttgtcaaagc ttacgatgga gaaaatgcaa
    17521 tccatcataa cggtacgggt gaaaaaggga cctctgttta taaagtgaag ggaatcggag
    17581 aacgtattta taaagaagga attcctcgaa ctgattggga ctggctgatt tatccagaag
    17641 gtctatatga cttgttgctg cgaattaaaa gcgattaccc acattacaac aaaatctata
    17701 tcactgagaa cgggatgggg tataaagatc agtttgaaga tggcattatt atggatcagc
    17761 cgagaatcga ctatttgaga gtctatttgg aatccttaag caaggcgata acagccgggg
    17821 taaatgtgaa ggggtatttt ttgtggtcgc ttatggatct tttctcatgg acaaatgggt
    17881 ataacaaaag gtatggcttg ttttatgttg attttgaaac ccagaagcgt tacccaaaag
    17941 aatcagctta ctggtacaag ttggtcagtg aaaccaaaac gattatttaa taagtgataa
    18001 tggaggaaga aaagatgaat aaagaggaat tacaaatgct tggttttgag atcgttgcat
    18061 attcaggcga cgctcgcagt acattattga aattattgaa agaagtaaga caaggaaact
    18121 ttgaacatgt tgattcggcg ttgaaagaag ccgacgaaaa tttaaccctt gcacataatt
    18181 cgcaaacaaa aattttagcg gaagaagcat ctgggaaaga gatggagatg ggattcattt
    18241 ttatccatgg gcaggatcat ttaatgacca ctttattgct gagagatctg attcaagact
    18301 ttattgtact gtacagacag aatcaaggat aggagaagaa cgctatggat ggaatcatta
    18361 aacaaatcga aaaagggaaa ccattttttg aaaaaatctc acgtaatagt tatttaggag
    18421 ccgttcgtga cggattttta acagcgatgc cagctatttt attttcaagc attttcattt
    18481 tgattgctgc ggttcctgag atttttggtt accaatggcc tgaatctgtc tccacatggc
    18541 tatggaaagt ctataattat tcgatgggtg ttgtagggtt actcgtttca gcaaccactg
    18601 ctcgctgctt ggctgattcg atgaatcgtc gcttaccgaa aaacaaagta atcaacacca
    18661 cctctgtgat gctggcctct gtggtaagtt tcctgttgtt aagcgtctct ccgattgaag
    18721 gtggattttc aacggaatat atggggacaa agggactact ggcttccttt gtcgctgcat
    18781 ttttaacagt taatttatat aagttttgtg tgctgaaaga tattaccatt aaaatgccta
    18841 aagaagttcc tggaacgatt tcgcaaacct tccgagatat ttttcctttc tctttttcag
    18901 tatttgcggc cgttttaatt gatttaggca ttcgacaaat cttccaaatc tcatttgcag
    18961 aagcaatcat tacggtgctg cagccgctat ttacagcagc tgacgggtac ttaggaatcg
    19021 ctattatctg gggagcgatg gcgctgtttt ggtttgtcgg tgttcatgga ccgtcgatcg
    19081 tggagccggc aattgcggca attatctatg ccaatgtgga aaccaatctg caattgttca
    19141 attccggtga acaagcttct aaagtactga cggtgggttt agggaatttt gtaggaacta
    19201 tgggaggaac tggggcaaca ttggttgttc cttttatctt cctactcttt gctaagtcta
    19261 agcagttgaa ggcagttggg aaagcttcct ttattccagt aagtttcgct gtcaacgaac
    19321 cattgctatt tgcggcaccc atgattttga acccttattt ttttattcca tttttgttaa
    19381 cacctgttgt aaacgtttgc ctgttcaagt tttttgtcga tgtattgggc atgaacagct
    19441 ttatgtacgt tttgccttgg gcaactccag caccgatcgg tttggttcta ggaacgggca
    19501 tgggtatctt agcttttgtt ctagcagcgg ttctaatcgt cgttgacttc ctgatctatc
    19561 taccattttg caaagcatac gatgctgtct tagtagagaa agaagcacaa aaagcagagg
    19621 tgatcgcaac cgaggaaggt gcttctgccg atgtacgtga ggaagtacct gtggcagcga
    19681 gtcaagttat cagcgatgca aataaagaaa tcaatgtgtt ggtactttgt gctggcgcag
    19741 ggacaagtgc gatgttagct aatgctttaa aaaaaggtgc atctgaatcg aaagcaccga
    19801 tcagtgcaat ggcgggtgct tatggttctc attacgaaat gctgcaggat tttgatatgg
    19861 tggtcttagc accgcaagtc aattcttact atgatgatat caaagaagac accgatcgct
    19921 taggaatcaa gctggttgcg acaaaaggag cagaatatat cggattaacg agagatcctg
    19981 aaaaagctgt tcagtttatt ctgaaccaat ttcaataaag gaggagccat aatgaaaatg
    20041 ggaatttggt tgcgtaaagc tgttttattt gcagctgtgt taggggcttt gggggctgtt
    20101 tcaaaaactg ttgaagcatc tgccagcgaa tttattcgcg gagcagatat ttcgatcctg
    20161 tcagatatgg aaaaatctgg tgcaaaatat tatgaagacg atatttcaaa agatgcctta
    20221 gagattttaa aaaataatgg tgtcaactat gttcgactgc gtttgtggca ggatccttat
    20281 aatagtaatg gagaaagtta tggagcaggg acaaatgatc tggaaacgac gattgacctt
    20341 gcaaaacgag caaaaaatct gggacttaag gtgctgctgg attttcacta cagtgatttt
    20401 tgggttgatc ctgggaaaca gaatcttcct aaagcatggc aaggtctgac ctttgaagaa
    20461 atgaatacgg cattgtacga ctatacaaag aacgttctgt cggaaatgaa gcagctggac
    20521 gtttatcctg atatggttca gatcggcaat gaactgaata gcggaatgct ctggccttat
    20581 ggcaagtctt ggggagaagg cgggggagaa tttgatcgat tggctgcttt tttgaagtct
    20641 ggtatccaag ctgttcgaga cactcaacct aaaacaactc cagtaatgct ccacttagct
    20701 gatggtggcg atacaggagc atttacttgg tggtttgatg aaatcacgag tcgaggtgtg
    20761 tcatttgatt taattggagt gtcgtattat ccgtattggc acggttcaat gaatgaactt
    20821 caaaccaata tgaatgcgat cagccaacgg tatggtaaag aagtgatcgt agttgaaaca
    20881 gcttatgggc atacaacagc gaatgccgat actatgccaa atgcttttgg tgaagccgaa
    20941 gcagctgcgg ggggatatga accaacaccg acaggacaag cagagtattt gctggatctt
    21001 gccgatcgga ttcaggcagt accgaataat cgcggggcgg gcttcttcta ttgggaacct
    21061 ctttggtaca acggaaatgt gagttgggca acacaagcag gtatgaatta tctcagtgtt
    21121 caatccacta tgggaaacga atgggacaac caagcgatgt ttgattttgc tggaaacgcc
    21181 ttgccaagct tgcgtgcgtt taaacaagca ggggcgcaaa taaatctagt taaaaatgca
    21241 agtttcgaaa gcgatggacc aacgaccagt ccttccaatt ggcaaacctg gtttcaacaa
    21301 ggaaccgcta agggtacgat caaaacggaa gcgggcactt tttttgggga ctacaaacta
    21361 actttttggc aggatcaagc atacgaggca tcagcttatc aaaatgttag tggcataagc
    21421 aaaggaactt acacattgag tgcctatgta atgtctgggg gagagtttga tactaatcag
    21481 ttatatatca aaaattatgg tggagcggaa ctaaatactg cggttgttaa aacaagtgaa
    21541 tggacaaaaa ttacaatcaa agacattcca attacaacag gtgtttgtga aattggggtt
    21601 tatagcaagg cttcaggaaa tgcttggacg aacgttgatt ttgtttcatt ggtgaaacaa
    21661 tagactgcgg atcgtttcga ttcaagatcg gaggttccct tcagccacat cgttagcgta
    21721 gaaggcagga atcgaacctg cacctcgcct ttgccaaaaa taaccagaaa cgatttccag
    21781 tcatttttac caaaacgagt aaagcaccag acttcatcta cactttgtat tatgctaaag
    21841 agagttcaac gatttcgtaa gaagcaatga aaaaattgca aaaagttgtg cagataacat
    21901 aaagtttgtt taagaaaggg taaccttaga attccgtcgt gaatatgatt tgagcactta
    21961 gatggataga aaaaagttct cggaaaacag ttcgtttttc gagaacttct ttttatgata
    22021 agaatgaaac aaattaactt atattatagt ctcttttggt agggaaccat cttattttta
    22081 atgaaaatca agcattttag ctattgaaga atgttgattt atagggattc cccctccata
    22141 ttatgttggc tctttattcc cactctgttt tcaacgtaaa gtgtataaaa cagaatgcga
    22201 tactatcaag ggagttaaaa caatatacag aacagtaaaa cactataaat aaaaataaaa
    22261 gtgcaataaa agtgcaattt ttgcacctaa tcatactctg tttatatatg aaaactagat
    22321 tctgaatcca atatttggtt aacgagaggc gttcgaattt gaacgtcttt tatatattac
    22381 tttcaaataa atatgaggta gaatactatc tattttcttt ttaacccaat tgttataatg
    22441 gagagtgtaa aatattttgt gtaaatagaa aaaaggaagt cccttctgta gaatagagtt
    22501 accacaacac attcacagaa gagaggactt ccctatgaac gattttacta cagaaattct
    22561 aaagactcta gcgaacaaag gcgatttgaa tgaattattc cgtgtccatt tggagaaagc
    22621 agtcaatacg cttctcaaaa cggagttaac ggctttccta gattacgaaa agtatgatcg
    22681 cattggtttt aacacgggta attctcgtaa cggctcctat gaccgtacgg tcaagaccga
    22741 gtatggggaa cttcatctcc agattccgcg cgaccgcaat ggtgagttca agcaacagac
    22801 tgttcctgct tatagacgga cgaatgacac gttagaggag accgtcattc acctcttccg
    22861 aaaaggtatt accatgtcgg aaatcgcaga cttgattgag aaaatgtatg ggcattacta
    22921 cacgccccaa accatgtcca atataacaaa atcatttaca gaagaggtaa cggcgtttaa
    22981 tgggcgggag cttcatgacc gttatgctgc tatttatatg gacgcaacgt atattccgtt
    23041 aaagcggaaa accgtcgcca aggaagctat tcatatcgca gttggcattc gcccggacgg
    23101 atcaaaggaa gtattgagct atgcgattgc accgactgaa tccatcacga tttgggagga
    23161 aattttattg gaccttcaag agcgcggttt gaaaaatgtc ctcctgttca tcacggatgg
    23221 cttaaagggg atggtaggag cgattagtcg gttctatccc aaagctcgtt ttcaacattg
    23281 ttgtgtacac gtttcccgta atatcagtca taaagtgcgt gtcgatgatc gtaaggaagt
    23341 ctgtgatgat tttaaaatgg tgtatcaagc ctcatctaaa gaggtggcgt tggaagcacg
    23401 tggtactttt gcggaaaaat ggaaaaccag ctatccaaaa gtggttgaat cgattctttc
    23461 gaacgatcac ttgctcactt tctatgattt ccccttggcc atacgcaaga gtatttattc
    23521 tacgaacttg attgaatcct ttaataaaca aatcaagaaa tacagccacc gcaaggaaca
    23581 gttccaaaat gaagagtcga tggaacgttt cttagtctcg tcttttgata cttacaacca
    23641 aaaattccta ggtcgcagtc ataaaggttt ccaacaggcc gaaggcgagc tcgaacaaat
    23701 gctaagccaa ctgattgaga attagaaaca atctgcagta gggaagaatc atttacacaa
    23761 aattattgac gctcccttat aatgagaact tttactaaca taatttatga taaacttact
    23821 atataatcat attaattaaa tagttaggag atctgcttat gtttatatct actcttcaaa
    23881 ttcgaaattt tagaaattat gaatccgaaa tctttcgatt cgataatgag acaaatacta
    23941 ttataggtga gaatgattca gggaaaacca atgctcttac tgcattgaga atcttattag
    24001 atgatagtta ttactatagt agtaaaacac taaaagaatc agattttttt catggaattc
    24061 aaaatgggtg gcaagggcat tggattatta tctctgcgac atttgaagga atcagcgaag
    24121 aagaatttga caatgagatt tgtgcttcca tttctttaaa ttctgaaagt caaacaatct
    24181 tagaagaatt gatttcaaat gcagataaag gtgtaggtag tatttctcta tttattagac
    24241 ctaacaaagc tattagaaag caattatttg atatttccac tgaattagac tctcatcaat
    24301 ttaacgaatt tagaaattct attcgtctat ccgactatga attttactac acatctaaat
    24361 caaatctaaa tttctgtatc aatgaaaatt atgataaatt ggtaggaaga ctcaatgaca
    24421 atatagctag taatcctgaa gaagacgatg aagctctgtt gggctcaaga attgacatgt
    24481 cagatatttt caagcatatt tcggttgttt atgttgacgc tcttcgagat gttcttagag
    24541 aaatgaaaaa caatcgaaac ccagtcaaaa gaatcatgga aacaattgaa tcaaaaatat
    24601 catcagataa tgttgagtca ttaaaaacaa ttatacagca attaaatgag actattacca
    24661 gtgttcctga aattaggaaa attggcgaaa atattaatcg gcaactaaat agcataattg
    24721 gttcagtgta ttctcctaat cttcttcttt cctcaactat gtcagatgat atgggttctt
    24781 tagcaaaatt tctttcaatg aagccagaac aaaatatcga cttagactta ctagggttag
    24841 gacatctaaa tatgatttat ttagctttga aaatagtcga atttgaagcg tgtcgttcaa
    24901 gagaactact taatattatg ttgattgaag aaccagaagc tcatattcat catcatattc
    24961 aaaaaactct gttcgaagga ttaaatcttc aaaaaaacta tactcagatt ctcatgacca
    25021 cacattctgt acatttagct gaagtgtctg aaatatctcg gatgaatgta ttaaaatcgt
    25081 ttaatggcaa atctatcgtt atgaatccag caaataatct gaactcattt gctaaagaaa
    25141 agttaaaaaa gaacaatcta aatctaataa acgcagtaga gagatattta gatgttaaaa
    25201 gaaatggatt gcttttttct aaaggtgtta tcttagttga aggagatgct gaagaaatac
    25261 ttattcctca aatagtaaaa aaattatttg gtataaactt agacgaactt ggaataggtt
    25321 tgattaatgt tggtagtact tcgtttgaat atatcgctag cttgtttgac gatgatagaa
    25381 tacagagaaa gtgtgctatt gtaactgatt tagatgaaca ggcaatccca tcagaacatc
    25441 gattgtataa aagcactgca caagagaaag gtcaagcaag aaaagaaaaa ttatcctccc
    25501 tttttaatga aaacaattgg gttaattgtt tttatgctaa tacaacattt gaaattgagt
    25561 ttttagatgt gaataaagaa aatattctaa cttatgtcag tccaattata gatgacgcat
    25621 tcaaagataa aaaaacaata actgcttata aagaagaaat acaaagtgaa caatatttag
    25681 aaagaaattt agcgatgtta aaactagcca atcatgttgg taaggggtgg tttgcaattg
    25741 agttaggtga aaaaattgac tttttaatca aaattcctaa gtatattctt aatgctattg
    25801 cttttgcatg tcaggaaata gtagatctct caatttataa aaaaattcta ctccatacaa
    25861 tcgacttgta cgaggaaact acagaattgt cagctttatc agctagttta aaaacaagtg
    25921 atacagctga tttgaatata gcaataattt ctttattaga gtactttggt aaagatgagg
    25981 atgttgtgta tctaatcaat cagatagaaa attacacaaa aatactttat gctgaggttt
    26041 caaaatgaca aatatcttcg aaaaaatcgt aaatgattta aatttagaac aaaaagatgt
    26101 tgtggaaagt gaagataacc tttatgtaaa tgcatgtcct gggagtggga aaacaagggt
    26161 acttacaagg aaaattgctt accaagcaat taaacacgat ggatcactaa aaagaataat
    26221 tgctatcaca tacactaata gagctgctga agaaattaag gaacgcctaa gtttacttag
    26281 tatagatgat gatattaata tttgggtggg gacaatacat cagttttgtc ttgaatttat
    26341 tatttatcct tttaaaatga atctctcaag agtatcaaaa ggatttacaa ttattgatga
    26401 ttatactcaa agagcttacg tcagccaaat taataatatg ctgaatttaa atctcaaaac
    26461 gtatgaactg aacaaaattg atttgtcact ttctcatgat tttaaaatta atgaagttca
    26521 gtatactcaa gtttctgaaa tgtatcacac tatgttggaa aaaaataaag aaattgattt
    26581 tgatatgatt ttactagtag cgtataaaat tttaactgta aacagtaacg ttgcacgtaa
    26641 tattgctagt aatataaggg cgatttatgt ggatgaattt caagatacta gagaattaca
    26701 atacaatgta atagctaaat tacttcaaaa caaccctcaa attcagtcaa tgtttgttgg
    26761 agatattgac caagcaattt atggatcatt agatggtata gctaaatcag ttgaagaact
    26821 agaaatgcta actagtcaca cttttcaatc aaagacgcta catggctgtt acaggtctaa
    26881 tcagagatta gtagacttct actctaattt tcagagttgt ccttacgaga ttgaaagcag
    26941 agggaataat aaaaacgacc gtggttttat aagttatgat tgcaaagtta ctaaggagga
    27001 tttacccaac ataataaaaa atataataaa ggaaaagatt gaaagcggca ttcatgaaaa
    27061 agatatttgt gtacttgctc ctcaacacta tcctctgttt tcactgggtg agtatttaaa
    27121 gaaagaatta ccgtattgca attttgatgc tataaatata tcaccaatta aggtaaataa
    27181 tcatagttta tttttcaagt tagcaatttt gtactttact gaatcaggtg aaaaggttcg
    27241 aagaagaaag ctaattgcaa acgatatttt aattatatta aaaaatgaat ttggaataga
    27301 agttaaagat tattttattg acttagattt attacagcta ataaacagtg tgaaaagaat
    27361 gtatagtgga gacgacaacg gtgttgattt atacaaattt gttaccaaac atatatttgt
    27421 taatcttaat attgacgaga aaaattaccc gtctattttt aagcactatc tattttttat
    27481 taacgaagta gaagatcgga taaaaaataa ttcattatct agtagtgtat ctagttttaa
    27541 gaagatgttt aaatctcgag atggtatcac acttactaca gcacataaag ttaaaggaaa
    27601 tgagtatgat actgttatcg ctattaatct tctaaacggg aaaattcctc attggaatga
    27661 aatttttaat gtttctgata aaggaattag ttcagcgaga aaactactat acgtaatttg
    27721 ttcaagagca aaaaataatt tatttctttt ctcagaggtg ggttatttta cgagtaaagg
    27781 agacttgctt tatcccactc aattattaga taaaatagaa tttgattata atatttaaaa
    27841 tacctaggta gtcgttgttt taaatatagt aggaatcgga cgctcagtac ggagcgtcta
    27901 tttttttacc caattttgaa aggaagtgaa cttatgaaaa taaaaaatca agaatcaaaa
    27961 ggtcgctctc ccccctttaa aaccatcaca caaataagat tcattcaaac ataaaaacag
    28021 aaaggatagg taaaaatatg gaacttaaat atgtcattcc caatatggaa aaaacatttg
    28081 gacaattaga atatgctggt gaagggaaca ttgaacaacg tcgaataaat ggcagaccaa
    28141 ccattctctc tcgtagttac aatttatact caacgattca acgagcagat gatattgttg
    28201 tcattcttcc ctccgaagct ggcgagaaac attttgaatc cgacgaaaaa gtcaaactca
    28261 tcaacccaca tattaccgca gaaggttata aaattggcaa tcgaggattt accaattaca
    28321 ttatgcacgc agacgatatg gtgaaagcat aagaaaggaa aggtgaaaaa atatgagatt
    28381 agcagaaggt attgtgattg aaaaagacca gacatttggc acattgaaat tttccgcctt
    28441 gcgtcgtgag gttcgtatcc agaatgaaga tggtacggtt tccgaagaaa ttaaagaacg
    28501 tacctatgac ttaaaatcaa aaggacaagg acgcatgata caagtcagta tccccgcttc
    28561 tgttcctttg aaagaatttg actacaacgc agaagtggaa ttaatcaatc ctgtggcaga
    28621 taccgtagcg acggctactt ttcgaggggc agacgttgat tggtacgtga aagcagacga
    28681 tcttgtatta aaaggacaga caaaacctca tcagaaatat aaaaaagaag cgtccgaaag
    28741 tgaaaaatag gtgttgctta tgacaaacac accattgatt ttgaaagaaa ttccgaagga
    28801 tgaagccatt tcttttattc ggcaatacca ttattcaaag atactcccta gattgtgtaa
    28861 atactttcta gggatttttt cagaagaaaa attattaggc gttgtggaat taggctgggg
    28921 tacgcaaccc ctacaaacca tacgcaaact ctttcccgac agttcccttc aaaccacaga
    28981 ttatttagaa attggaaaga tgtgttttct tccagaaatg aatcaaacca attattttgg
    29041 cagtcaagct ctttcagctc taattaagtg gttgaaagaa catacggact gtcatttttt
    29101 atatacactg gcagatggaa ttgagggaaa atgtggctat gtctatcaag cgtccaactt
    29161 cttttactgc gggtatttca agacaagtgt ctatcgtgat aagcagtcat gggaaaagat
    29221 acaccctcga agtgctcgcc ttttattaga ggaaaatgct cgctttgaac aggtagagaa
    29281 aaagcattgg ctttcccagg cattttgcga atacaaaggg attgagaaaa tcaatggacg
    29341 catgtttcgc tatctctatc cgttgacaaa agaagcgaaa aaactgttag gacacactct
    29401 ctatcgacgt cattattatc cgaaagaaaa aaatttacgc tttgaaaagc gaatcgctta
    29461 tcagaaatat gaagccattt ctcaaccaac ttttgataaa caggctcgta tctacaatac
    29521 acaattattt tgataggtgg aaaggaggat ttttatgcca ttgcttcatg acagaggaga
    29581 gcgaatcaaa gccaacgata agcacttggt ctatcatttt gtactaattt cattgctggt
    29641 agtttttcta gtcgtcctac tcccttttca ttggagagcg ttacaatcgg tgaactggca
    29701 agcagttcag ttagatacat ttttctatca acttcatacg ccatactttt ggagcagtat
    29761 tctcactgct ctttttgtat gcggacttat cagctggctt tattatcact atcgtataga
    29821 ttctataaaa cagttagagc accgtcaaaa gttagctcgt atgattttag aaaatcattg
    29881 gtatgaagcc gaagaaactc aatcagaggg attctttaaa gatttaccca gcaagaaaac
    29941 caaacaacga atccgccatt ttcctaaaat atattatcga atgaaagatg gactaatcca
    30001 tattcatgta gaaatcacct tagggaaata tcaagatcag cttttgcacc tagaaaacaa
    30061 actagaaaca ggattgtatt gtgaattagt ggaaaaaacg ttgaaagatt cttatgtaga
    30121 gtatgtccta ctttatgata ctattggcaa tcgaattggc attgaagacg tggtagctca
    30181 agatggacgt gtcaaattga tggaatcggt ctattgggag ttcgattctc tccctcacat
    30241 gttaattgct gggggcactg gtagtggaaa atcctatttt attctcacat tgattgaatc
    30301 tctcctgcat acaaatgcaa agctttatat tcttgaccct aaaaatgctg atttggcaga
    30361 cttaggggcg gtcatgaata atgtctatta ccgtaaagaa gatatgttgg catgtatcaa
    30421 tcaattttac gaagatatga ttgatcgcag tgaaaccatg aaacaacacc ctaactataa
    30481 aacaggagaa aattatgctt atcttggttt gtctgccaat tttcttattt ttgatgaata
    30541 tgtggcaatg atggatatgc taggtcgtga aagtaccacc gttattcata aactcaaaca
    30601 aattgtaatg ttaggacgac aagcaggctt ctttttaatt cttgcctgtc aacgtccaga
    30661 cgcaaaatat tttagtgatg ggattcggga caactttaac ttccgtgtgg ctttgggtcg
    30721 tatgtctgaa atgggctttg gtatgatgtt cggaagcgat acacaaaaac aatttttctt
    30781 aaaacccatc aagggaagag gttacgtgga tacaggaaaa agtgtgatta gtgagtttta
    30841 tacacccctt gtaccaaaaa ggtacgattt cttaggagaa ataggaaaag tcattcaaaa
    30901 aaagcaatct gagcctgtac cgcacgaagt gaaaggtaca ggttcagact tgctttgatt
    30961 ggtgtggcgt tagccacgcc aatcctttag ccccctctat ctaacagggg ggcacaaata
    31021 caactgaaaa caggcaattc tcatcaacca atgttgatgg tacaaaggat tgcggaaaat
    31081 atgaaaggtg taaactcaag ggtcatagtt tacatctttt ttagattgga ggtttgcgaa
    31141 tggaaaacga aaaaatttgg attaaggaat tgaaagaaaa gcgactggct tatggcgttt
    31201 ctcaaaataa actggcggtt gcttctcata ttaccagacc ttacctatcg gatattgaaa
    31261 caggcaaggc agtccctact caacaggtga aagaagattt actgaacgct ttggaacgct
    31321 tcaatcccga taacccattg gagatgttat tcgactatgt acggattcga ttccctacca
    31381 atgatgtggc tcaaattatc ggtgaggttt tacgtttgaa tatggattat atgctacacg
    31441 aagactttgg ctattattct tatcccgaac actatcgttt cggtgatatt gttgtcctag
    31501 tctctcatga tgtaagtaaa ggtgttttat tagaactgaa aggaaaaggc tgtcggcaat
    31561 ttgaaaactt tctattggct caacatcgga gctggtatga tttctttcaa gattgcatgg
    31621 aacataaagg tattttcaaa cgcttggatt tagctatcaa cgataaaaca gggattttga
    31681 atattccaga tttggcaaag aaatgtaaac aggaagaatg catttccgtc tttcgaagct
    31741 ttaaaaatta tcgcagtggt gaattggttc accgtgatga aaaaccatac atgggaaaca
    31801 cactttatat tggctcatta aaaagtgaag tctatttctg tatatatgag aaagattatg
    31861 agcaatacgt gaaaaatgat gttcccttag aagacgcaga agtaaagaat cggtttgaaa
    31921 ttcggttaaa aaatgaccga gccacccaag ccatgaaaga tttattggca catcaacagg
    31981 cggaaaaaac agcctttgaa attatcaatc gctatatccg ctttgcagat aaagacgaca
    32041 ctaaacgtcg gagtgattgg aaaactaatg aacgttggga atggtttatc ggtaaaaatc
    32101 gtggagcatt acgcctaacc acacagccag agccttattc ttttgaacga acattgaact
    32161 ggttacatca tcaagtcgcc cctacattaa aaatcgcaag cattttagat gttctcaacg
    32221 gtacaaccat tatctccacc atgattcagg aagccaaact gacagaaaaa catgagaaat
    32281 taattgaaca acaacatcta gccatggagg atttgattac atgagccttt taacaaaaga
    32341 attaaagaag ttagggtttc agtgtggcat tgagtttcaa gcgtatatac aaaatacagg
    32401 gaaatatacg tctctcatta ttgagggaaa acggcaagct ggtgatacca tctataccta
    32461 tgatttttac aaggtgagat tttataataa ttataccaat cgagtaaccg tctatggaga
    32521 gcatttaacc ccttttcaat tattaaggcg tgtaaaaagc tatatctatt atcgagaaaa
    32581 atatcttaag gaaaggagga caatcacatg aattttgggc agaatttgta caactggttc
    32641 ttggataatg cacagtcatt ggtattgtta gcgattgttg tcattggact gtttttaggt
    32701 tttaaacgag aattttcaaa actcattggc ttcttgatta ttgctctaat cgccgtcgga
    32761 cttgtgttca atgcagctgg cgtgaaagat gtgttattaa acttatttaa ccgaatcatt
    32821 ggtgcttaac agatattttt catgataaaa taggaagtat atattttgtt atgaaaggat
    32881 ttatgaatat gtttacaaat gatcaattta aatctctttt aattaaaaca ttagaaagaa
    32941 agagctcttc aaattattat gaaggaggta acgagattgt ttctaaaatg aaaatatcag
    33001 aggtaacttt agatgagact gacgatttta cttattataa aggatataaa aagggctgga
    33061 atacgctttg cacatatctt aaaataagag ttccttttga tgatttagac ttttttgaaa
    33121 gtcataaaga tttgattacc cagacagcaa gctccatata tgataaacaa ggtgataatg
    33181 tattagtgga tactatttta gtaccacttc cagagaatta cgaagtaatt aactttagtc
    33241 agttgaaaat tagtgatgtg gtttcacaag ctattgaaga cgctgagtct tttatgagta
    33301 atggtgaata tcaacgtgcc tttgacagag ttcacactgc ttttcatgga tatttaattg
    33361 aaatactaaa aaaatatgaa attacagttc ctagagacga aaatctatct aaattatata
    33421 gtaggataca acaattaatt gaaaaagaaa ttcaacctac tgaacttgct gatatagtga
    33481 agacaacgat aaggtcatca aatggaatga ttagctctct taatgaagca agaaataggc
    33541 attcacttgc acaccctaat acaaatatta ttggaaagag agaagcaaaa ctaataatag
    33601 gtatttcaag tacagttaca gattatatta gcggatacct tgataagtag cgtcattaac
    33661 tgattgctac tttttttatg actattttta taaaaaggat gtgataagaa ttggaaatga
    33721 aagtctatgt ggcaaattta ggacggtata atgaaggtga attagtcgga gcatggttta
    33781 ctccaccaat tgatgaggaa gaaatggcag aaagaatcgg tttaaatgag gactacgaag
    33841 aatatgcaat acatgatttt gagcttcctt ttgatgtgga cgaatacacg ccgattagtg
    33901 aaatcaaccg tctatgtgaa gccattcaag aaatcgaagg tacacctatt tataatgaac
    33961 tcaaagaaat tcaaggcatg tggtttagta gcttggaaga attactggaa aacaaagagg
    34021 atattcattg ttatagtgat tgtgattcga tggaagatgt cgcccgttat tacgtggaag
    34081 aaacaggaca gcttggtgaa gtcccttcca atttacaaaa ttatattgac taccaatctt
    34141 tgggacgtga tatggaaata gagggtaatt acctagttac ctctcatggg gtatttgaat
    34201 attgtcagta agtttaacaa tccgtgttat aattaaatta ataaatagca cggagaggta
    34261 taaattatgg taaatgtatt tgaaaatctt aaactattaa aactagacat gaaagataaa
    34321 ggctgggtga tagattcctt ttactttcgt tacaaacagc aaaattatat tgtacttgtt
    34381 aagctatttg aaaaagagga aaaagttcca gaatatgctc tgttaaaact agaattttta
    34441 aaggaaaacg atttttcaga tatgctagct gtctatgcta attcggtaaa attatataca
    34501 gacacgaaaa caataagaga atattttggt atcgaataca gtagtaactt gggagatgtt
    34561 ttatttcaat ttagccaaac tcttgctcga tttattccga cagaagttag tgagaagaaa
    34621 aatgaagacc aaaaagaagc catgtgtttc tcattaagtc aaagtgattc agaagatccc
    34681 agaaaaaaat attgtttttc agttagaaga aatcctttaa agggaaatgg tgagctggga
    34741 aaacgaagtc cttttaatga caataaaacg agattgtata gacccagctt atatgaacga
    34801 ttagggtcag ataccaattt aagtttcaga tattcaatga atccagatga tgaagaaact
    34861 gatgaaggta ttattgcaaa atggacaaaa aataaaattg aatagttaaa gcattgaaag
    34921 agcaatcacc tattttgatt gttctttttc tatttttacg gaaaggacgt gaagacatga
    34981 aaaaaataag aagttacacc agtatttggt cagtagaaaa agtgatttat gcgattaatg
    35041 atttacaact ccctttccca attaccttta cgcaaatgac atggtttatc gtttctttgt
    35101 ttgtcgtcat actctttggc agtttaccac cactttctct gattgacgga gcactactca
    35161 aatacttagg gattcccatt gttctcactg ggttcatgtg ccaaaagacc tttgacggaa
    35221 agaaacccta tggatttcta aagtctatca ttacctatgc tctacgccct aaagtaacct
    35281 acgctggcaa acaaatacga ttaaaaaaag aaacaatgaa tgaatcgatt acgattgtaa
    35341 ggagtgaaca actagatggc atatccgatt aaatatatgg aagataatct cgtatttaat
    35401 catgatggcg aagtattcgc ttattatgaa ttaattccct ataattacag ttttttaagt
    35461 tccgatgaaa aaattcaagt tcatgataat ttctgtcaat tgattgccca aaatcgtgat
    35521 ggcaagattc atgctctgca actagcaacc gaatcaagta ttcgagccac acaggaacaa
    35581 tcgaaaaaag aaatcactgg acgattaaaa gagatagctt acgaaaaaat agatgaacag
    35641 accgaagcgt taatttctat gattggtgac aatcaagtag actatcgctt ttttattggt
    35701 tttaaactct acctcaatga acaggaagta tccgtaaaaa atgttgggaa agaaattcaa
    35761 aaagcatttg cggaattctt tcatggtgtg aatcacaaat taatgggcga ttttgtttct
    35821 atgcctatgg cggaaatcga acgcttttct aagatggaac agttgttaga aagtaaatta
    35881 tccagacgct ttcagattcg taaattggag aaagatgatt ttggttatat tattgaacat
    35941 ctttacgggc aaacaggtgt cccttttgat gactacgact accatttacc gaaacgatac
    36001 gaagataaaa aagtgttggt aaagaaatac gatattctca aacccactcg ctgtttaatc
    36061 gaagaaaatc aacgctattt acggattgaa caggaagata ccactactta tgtggcgtat
    36121 tttacaatta atagtattgt tggtgaattg gatttcccta atagtgaaat tttttattac
    36181 cagcaacagc agtttacttt ccccgttgat accagtatga atgttgaaat tgtcgccaat
    36241 aaaaaagcct tatctaccgt tcgcaataaa aagaaagaac tgaaagattt agatagccac
    36301 gcatgggaaa gcgacaacga aaccagtact aatgtggtgg acgctttaga atctgtcgat
    36361 gaattagaaa gtaatttaga ccaaaccaaa gaatctatgt acaaactaag ctatgtggta
    36421 cgggtatcag caaagaattt ggacgagctg aaacaacgtt gtgatgaagt aaaagatttt
    36481 tatgatgatc tgtctatcaa attagtacga ccttttggcg atatgctagg cttgcataat
    36541 gaatttattc cctcaagcaa gcgatatatc aatgactata ttcaatatgt cacgtcggat
    36601 tttttggctg gtcttggttt cggtgctaca caaatgattg gtgaaaattc tggtatttac
    36661 gtcggttaca atctcgatac aggcagaaat atttatctcc aacctagcct tgcaagtcaa
    36721 ggaattaaag gctctgtaac aaatgcatta gcgtccgctt ttattggttc attaggtggc
    36781 ggaaaatcat tctctaacaa tatgctggtc tattattcag tactctttgg cggacaggca
    36841 accattgttg acccaaaagc tgaacgtggt gaatggaaag aaaccttacc tgatattgcc
    36901 catgaaatca atattgttaa tctcacgagt gagaacgaca ataaagggtt acttgaccct
    36961 tatgtgattt taaaactccc gaaagattca gaaagccttg ccattgatat tctcacgttc
    37021 ttaacaggta tttccagccg agatagcgag aagtttcctg tcctgcgtaa agctattcgg
    37081 agtgtaactc aaagcaaaca acgagggttg ttattggtga ttgatgaatt gcgaaaagaa
    37141 gatacgccca tcagcaatag tattgctgac catatcgaaa gctttgtcga ttatgatttt
    37201 gcccatctgt tattcagtga tggcagtgtc acccaatcca ttagcttgga aaaacaactc
    37261 aatattattc aagtggcaga tttggtctta cccgattcca atagctcttt tgaagaatac
    37321 actaccatgg aattattaag tgtggcaatg ctgattgtca ttagtacctt tgctttagac
    37381 tttatccact ctgaccgttc catttttaaa attgttgacc ttgatgaggc ttggagtttt
    37441 ttacaagtgg cacaaggaaa agctctatct atgcgattgg ttcgagcagg acgtgccatg
    37501 aatgcagggg tctatttcgt tactcaaaac gcggacgatt tactagatga aaagatgaag
    37561 aataatattg gcttgaaatt tgcctttcgt tccactgacc tagtggaaat caaaaagacc
    37621 ttagaatttt ttggtgtgga caaggaagat gaaaacaatc agaaacgctt acgtgaatta
    37681 gaaaatggac aatgtttagt ttcagacatc tatggtcgtg taggggttat gcagtttcac
    37741 ccgatttttg aagagttgct tcatgccttt gataccagac cgcctgtgcg aaatgaggtg
    37801 gaagaatgac acaaaaacaa aagaaatttc ttcactatag ttggataacg ctcctttgtg
    37861 tgggcgttat tttactttta ttaggcacgc tgggcaatgc agtacaagcg acaggcttag
    37921 ttgatgaaac caaatactcc aagtatggtt tgaatcaata tcagcttgat tattatgtgg
    37981 acaatagctg gggctggctt ccatggaatt ggagtgatgg cataggacaa tcggtgatgt
    38041 atggattgta tgccattact aattttattt ggacaatttc tctttatcta tcgaacgcca
    38101 caggttattt ggtgaaagaa gcctatgcct tagatttcat tagtcaaaca tcccaagcaa
    38161 ttggcgaaaa catgcagacg attgcaggca ttacaccgaa tggcttttcc agttcagggt
    38221 tttatgtcgg tttcctcctt ctttttattc tcattgttgg aatctatgtt gcctatacag
    38281 gattggttaa gcgagaaacg acgaaagtga tacgagctgt tttaaacttt gtcgtggtgt
    38341 ttattctatc ggcttccttt attgcgtatg cacccgatta tattgcgaaa atcaatgatt
    38401 tttccaaaga tgtcagtacc gccagtctga atattgggac aaaaattgtt cttcctaaca
    38461 cggatagtca agggaaagac agtgtggaca tgatacgtga cagcttattc tctgttcaag
    38521 taaagcaacc atggctattg cttcaatatg gctccactga tgtagaagcg ttaggggaag
    38581 aacgtgtgga aagtttattg tctaccagtc cagatacaaa caatggtgaa gatagagaaa
    38641 atgtagtgat tgaagaaatt gaagatcatg acaataccta tttaacccta ccgaagacga
    38701 tttccagatt aggaacagtc ttctttttgt ttgtattcaa tattggtatc tcgatttttg
    38761 ttttccttct aacaggaatc atgatttttt cgcaagtgct atttattatt ttcgctatgt
    38821 ttttgccaat cagtttccta ttaagtatgt tgccgagttt tgatggcatg ggcaggcatg
    38881 ccattaccaa gttatttaat gtgattatga caagggctgg tattaccttg attattaccg
    38941 ttgcctttag tatttccact atgctttatt cgctgtcagc aagttctccc tttttcatga
    39001 ttatgttttt acaaattgtg acctttgcag ggatttattt taaactgggc gatttaatga
    39061 gcctattcac tttacaaagc aacgactctc aaaacatggg cagacaagtg tttcgcagac
    39121 ctcgtcaaat gatgaatcga caaaccagaa gatttcaacg cagtatgagt aagatttttc
    39181 gtggaaaagc agggtcaacg aagcaaaaga caatgacaac aaagcagaat caaaccaata
    39241 aagctaacca tactcgtcaa aatgaacgga ctacaccaaa gaaagaaaca gcactttcca
    39301 atgtgaaaaa gcaacaaggc ggtatcgggc aacgattagg tgaaaagaca gcccatgtat
    39361 tggacacgaa aaatcgtcta gtcaataaaa gaaagcaggc aaaacaacag atacaggcga
    39421 caccaaccca tgtcaaatat gcccttcatc aaggaaaaga gaatgtcact cataatgtaa
    39481 cagactttaa acaatctatg gcagatacca aagctcaacg caaacaacag cgtatgaacc
    39541 agcaggagca aaaacgaaag aacattgctc aaaagcgttt ggagctggat aaagcaaaag
    39601 aaaaaagacg aaaagaaaac ccatcatctc ctgttcagaa taaccagcag catgttcgca
    39661 agcgtcagca aaccgtcacg cctgttttgt cacaaaacac taaacgtgaa aatcatcagc
    39721 gaccattgaa aaaagagcaa aaacaaaaga caccaccgaa gcaattcaat cagaggaagc
    39781 taaataaaag aacggataag cgaggacaac aaacatgaag ctaaaaataa cagtgatttg
    39841 tagtgtggtc ttctctctct tcttttttct cttattattc ctagtcattt tctttgcaga
    39901 tgatacggac agtggcgaaa acaacaagga ttcttctatt ccacaaggcg gtgtgaccgt
    39961 ttcaccagaa gtgttggcac accgtccgtt gattgaaaaa tatggtaagg aatatgggat
    40021 tgaagactat gtttcttata tcttagccat tatgcaagtg gaatcgggcg gtacagcaga
    40081 agatgttatg caaagttccg aatctttggg tttaccgccc aacagtttga gtaccgaaga
    40141 gtccatcaaa caaggggtta aatatttcag tgaattatta accagtgcag aacaacaagg
    40201 agtggatata gattctgtta tccaatctta taattatggc ggtggctttt taaattatgt
    40261 gaggagtcat gggaaaaaat atacctatga attagccgaa cagttttcta aagagaagtc
    40321 gggtggtcaa aaagcagact atcccaatcc tatagccata cctgtgaatg gtggttggcg
    40381 atataactat ggaaatcagt tttatgttca gttggtttca caatacctaa ccgacacttc
    40441 cccaacagaa tttgatgatg aaaccgtgca agtcattatg gacgaagcct tgaaatacga
    40501 agggtttccc tatgtctttg gaggggcgtc gcctaccacc tcttttgatt gtagtggctt
    40561 aatacaatgg gtgtatgaca aagctggcat ttctctccct cgtgtcgccc aagatcagta
    40621 tgacgctacg caagaaatct ctatggaaga agcccaagca ggcgacctta tcttctttca
    40681 ttcaacctat aatgcaggca catatgtgac tcatgtggcg atatatttag agggaaatcg
    40741 cttttatcat gcaggcgacc caatcggtta tggcgactta agcagtcgtt attggcaaga
    40801 ccatctgatt ggggctagac gtgttattca taactaagaa aggaatgaat caaatgatta
    40861 taaaaatcga gcgaaaagaa aagaaggaga aaccaaagaa atctaaaaaa ccgagcaagc
    40921 aaagaaaagt tccgatgatt aaagttggca cgcataagaa attaaccttt gttttatggg
    40981 ttttattgat tggtagtgtc ggatttggaa tctataaaaa ttttactgcc attgacactc
    41041 atactgtccg tgaaacagaa atcattaaac agcagattgt cgataccaac caagtagaaa
    41101 gttttgtgaa atcctttgcg aaagactact tttcgtggca acaatcccaa gaagccattg
    41161 ataaacgaaa tgaaaaattg acccattatt tgacggaaga attgcaggta ttaaatgaag
    41221 aaatgattcg taaggatatt cctactagtt ctagtgtgaa cgatatacag gtgtggcagg
    41281 tttctcaagt gaatgagaac acctttgagg tactgttttc tgttgaacaa gttattaccg
    41341 aagacaaaga caaggaaact atatcgtcta gctttcatgt ggttgtccat atagatgaat
    41401 cagataatat ggttattatc aaaaatccga caatgagtaa gaaacctcaa aaatcggact
    41461 accaacccaa acagctagaa agtgaccata cagtagatac agaaacgatg gacgaaatca
    41521 ctagcttttt agaaacgttc ttccagctct atcctaccgc cacagaaaaa gaattgactt
    41581 actatgtaag taatcatgtg ttacctatga ttaacaagga atatgttttc gaggaattgg
    41641 tgaatcctat ctttaccaga aaagataacc aagtaatcgt gaatgtcgct gtgaaatatt
    41701 tagatcaaga aacaaaggca acccaaatct cgcaatttga gttgatatta gaaaaacaag
    41761 ataattggaa gattgtaaaa taacaaatat tggtacatga ttacagatac tttgtaatca
    41821 tgtactcttt ttgataaaaa attggagatt cctttacaaa tatgctctta cgtgctatta
    41881 tttaagtgac tatttaaaag gagttaataa atatgcggca aggtattctt aaataaactg
    41941 tcaatttgat agcgggaaca aataattaga tgtccttttt taggagggct tagttttttg
    42001 tacccagttt aagaatacct ttatcatgtg attctaaagt atccagagaa tatctgtatg
    42061 ctttgtatac ctatggttat gcataaaaat cccagtgata aaagtattta tcactgggat
    42121 ttttatgccc ttttgggttt ttgaatggag gaaaatcaca tgaaaattat taatattgga
    42181 gttttagctc atgttgatgc
//
LOCUS       DAMEET010000011        41437 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.011, whole genome shotgun sequence.
ACCESSION   DAMEET010000011 DAMEET010000000
VERSION     DAMEET010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 41437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.011"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            214..498
                     /locus_tag="PB780_000534"
     CDS             214..498
                     /locus_tag="PB780_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HDE0732609.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(779..1198)
                     /locus_tag="PB780_000535"
     CDS             complement(779..1198)
                     /locus_tag="PB780_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HDE0732610.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(1200..1784)
                     /locus_tag="PB780_000536"
     CDS             complement(1200..1784)
                     /locus_tag="PB780_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HDE0732611.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="PB780_000537"
     CDS             complement(1831..3306)
                     /locus_tag="PB780_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDE0732612.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="PB780_000538"
     CDS             3487..3798
                     /locus_tag="PB780_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732613.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="PB780_000539"
     CDS             complement(3913..6249)
                     /locus_tag="PB780_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDE0732614.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="PB780_000540"
     CDS             6391..7059
                     /locus_tag="PB780_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDE0732615.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7062..8105
                     /locus_tag="PB780_000541"
     CDS             7062..8105
                     /locus_tag="PB780_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDE0732616.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8342..9223)
                     /locus_tag="PB780_000542"
     CDS             complement(8342..9223)
                     /locus_tag="PB780_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDE0732617.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9382..10110
                     /locus_tag="PB780_000543"
     CDS             9382..10110
                     /locus_tag="PB780_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDE0732618.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10266..10583
                     /locus_tag="PB780_000544"
     CDS             10266..10583
                     /locus_tag="PB780_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDE0732619.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10576..11184
                     /locus_tag="PB780_000545"
     CDS             10576..11184
                     /locus_tag="PB780_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HDE0732620.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11239..12030
                     /locus_tag="PB780_000546"
     CDS             11239..12030
                     /locus_tag="PB780_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDE0732621.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            12168..12656
                     /locus_tag="PB780_000547"
     CDS             12168..12656
                     /locus_tag="PB780_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HDE0732622.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12707..13579
                     /locus_tag="PB780_000548"
     CDS             12707..13579
                     /locus_tag="PB780_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDE0732623.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13755..14117
                     /locus_tag="PB780_000549"
     CDS             13755..14117
                     /locus_tag="PB780_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDE0732624.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14126..14878
                     /locus_tag="PB780_000550"
     CDS             14126..14878
                     /locus_tag="PB780_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDE0732625.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14892..16694
                     /locus_tag="PB780_000551"
     CDS             14892..16694
                     /locus_tag="PB780_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDE0732626.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16971..18011
                     /locus_tag="PB780_000552"
     CDS             16971..18011
                     /locus_tag="PB780_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDE0732627.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(18063..20207)
                     /locus_tag="PB780_000553"
     CDS             complement(18063..20207)
                     /locus_tag="PB780_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDE0732628.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(20564..21937)
                     /locus_tag="PB780_000554"
     CDS             complement(20564..21937)
                     /locus_tag="PB780_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDE0732629.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(21937..22332)
                     /gene="rbsD"
                     /locus_tag="PB780_000555"
     CDS             complement(21937..22332)
                     /gene="rbsD"
                     /locus_tag="PB780_000555"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDE0732630.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(22360..23277)
                     /gene="rbsK"
                     /locus_tag="PB780_000556"
     CDS             complement(22360..23277)
                     /gene="rbsK"
                     /locus_tag="PB780_000556"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDE0732631.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(23385..24398)
                     /locus_tag="PB780_000557"
     CDS             complement(23385..24398)
                     /locus_tag="PB780_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDE0732632.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(24479..25078)
                     /locus_tag="PB780_000558"
     CDS             complement(24479..25078)
                     /locus_tag="PB780_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDE0732633.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            25264..25965
                     /locus_tag="PB780_000559"
     CDS             25264..25965
                     /locus_tag="PB780_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDE0732634.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            25975..28686
                     /locus_tag="PB780_000560"
     CDS             25975..28686
                     /locus_tag="PB780_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDE0732635.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            29137..30765
                     /gene="treC"
                     /locus_tag="PB780_000561"
     CDS             29137..30765
                     /gene="treC"
                     /locus_tag="PB780_000561"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDE0732636.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(30825..32006)
                     /locus_tag="PB780_000562"
     CDS             complement(30825..32006)
                     /locus_tag="PB780_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDE0732637.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            32258..33781
                     /locus_tag="PB780_000563"
     CDS             32258..33781
                     /locus_tag="PB780_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HDE0732638.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(33850..34578)
                     /locus_tag="PB780_000564"
     CDS             complement(33850..34578)
                     /locus_tag="PB780_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDE0732639.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(34591..35871)
                     /locus_tag="PB780_000565"
     CDS             complement(34591..35871)
                     /locus_tag="PB780_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDE0732640.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(36012..36614)
                     /locus_tag="PB780_000566"
     CDS             complement(36012..36614)
                     /locus_tag="PB780_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDE0732641.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            36859..37065
                     /locus_tag="PB780_000567"
     CDS             36859..37065
                     /locus_tag="PB780_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732642.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            37377..38096
                     /locus_tag="PB780_000568"
     CDS             37377..38096
                     /locus_tag="PB780_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDE0732643.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(38177..38860)
                     /locus_tag="PB780_000569"
     CDS             complement(38177..38860)
                     /locus_tag="PB780_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HDE0732644.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            39078..41264
                     /locus_tag="PB780_000570"
     CDS             39078..41264
                     /locus_tag="PB780_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDE0732645.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT        13441 a         7347 c         8315 g        12334 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccgtat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatggttt ttcaccatct ttataataaa ctggttcttt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcattg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaagaaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg attcgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atttgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt
     7081 cacaattatg gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt
     7141 gttttctcgg ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg
     7201 gaaaaccaac gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag
     7261 gaatatcgat ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata
     7321 tgagtctggt agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag
     7381 aagtacagca actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga
     7441 cactgaggaa tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa
     7501 tccaaggata tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat
     7561 ttttatatcg gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa
     7621 aattgaacaa actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg
     7681 aacaaatcag agaagtaatc gttttttttc agcctaaatg ggagaaagaa cagctagaat
     7741 tggatatcga acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt
     7801 ggctgaatat catggataac gccataaagt acaatcaaat caatggacag attcatatca
     7861 aactatttga gacagccaca gagattgtac tggaagtgac cgattcaggt gtaggaatga
     7921 atgaagagac cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa
     7981 tttctgggaa tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac
     8041 gtattgatta tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac
     8101 aataggaaaa agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc
     8161 gtttttttgt gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc
     8221 ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga
     8281 gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg
     8341 cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac
     8401 cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag
     8461 tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat
     8521 ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa
     8581 atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga
     8641 gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat
     8701 gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac
     8761 ttggtggggc ctggaaactt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat
     8821 tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt
     8881 tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga
     8941 tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt
     9001 attgttctac gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga
     9061 tttttctatg ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct
     9121 gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg
     9181 tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta
     9241 ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac
     9301 aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta
     9361 gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg
     9421 tttggcggac taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag
     9481 ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg
     9541 aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg tttgattgtt
     9601 catttaggta atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc
     9661 ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc
     9721 attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca
     9781 ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt
     9841 gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat
     9901 attttgttct tattggcaaa atttatttat ccattgacta caccagacat gatcttagat
     9961 tttaaagctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa
    10021 atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg
    10081 tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg
    10141 actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac
    10201 tatattatat gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg
    10261 acgtaatgca catccagatt ccaactgttc ttcttgatgg tactgtgcta gcagcattaa
    10321 gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca
    10381 gtgaatctac actatatccg gttttgagaa gattgaaaaa agaaggatat cttgagacct
    10441 atgatgaacc ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc
    10501 gtcatttaga agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat
    10561 tggaggagag cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt
    10621 cctgaagacg atcgttttga tttgatccag tattatgagg aatatttttt agattctgga
    10681 aaaacattag atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta
    10741 aaaatcagtt acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa
    10801 agacaaattc gcctgatttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt
    10861 ccactagcac tggggtttat attggttatt gggggactat tggttgcttt actagcgctc
    10921 atttttagta tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc
    10981 tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt
    11041 ctgttcgcca caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg
    11101 cttttttatg tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga
    11161 gatctgcatt cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg
    11221 attaccggta ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta
    11281 ggcgctcaaa agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag
    11341 acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg
    11401 gtcatacgcg gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa
    11461 ataaaggaga aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc
    11521 attagtttag aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta
    11581 tctgaattga caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa
    11641 aaagtgaata ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca
    11701 ataactgcga aagatgaatt aggctcactt cgattagatg aggccaatgt ggataacttg
    11761 atcgtcacag gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga
    11821 aaagtcatcc taaacggtgg caatttatct ttgcatgaga tggaaaacag tggctatact
    11881 atccaaacgt ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg
    11941 ttagaacaag gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg
    12001 acggatcaag aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa
    12061 aacagcaaag atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt
    12121 aaaataagaa agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag
    12181 aaggtcaaag agaaaagtta aaaacagaaa tggaaagatt acatacttat atcactcaac
    12241 taagccaaac cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt
    12301 cagaaggtcg ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga
    12361 agaaagagct ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg
    12421 ggaagaagga acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga
    12481 tggatctaga ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa
    12541 aattggatta tttaccgcag gcagccggag ggacacaggc aagcggttgg tacatcactg
    12601 aagacaaaga gttagaacta gaaggtgtaa tggcgcgtat tcgaaaaaaa aattaaaaaa
    12661 gtaacattag atatttattg aaggataaag gagcgcagga agtagcatga gagaaaaaag
    12721 agcattttat atcaatggtt atgcagggat tgtcggattg gtcgtattag ctttagttgg
    12781 attgtttttc ttctatttag gtatgtggca agcgaaagtt tgggcacttt ttttaagtat
    12841 ttttctttgg ttgatcacgt tattgttgtt aagttcggca actgtagtca gtcctaatca
    12901 agcaaaagta attttgtttt ttggacaata tttaggtacg attcgagaaa atggcttttt
    12961 ccttacgata cctttggcac aaaaaatgac tgtttcgttg aaagttcgga atttcaatag
    13021 ttctgtttta aaagtcaatg atttggatgg aaaccccatc gaaatctcag cagtagtagt
    13081 ctttaaagtc gttgatacag caaaggcatt attcgatgta gcgtattatc aagattttgt
    13141 agaaatccaa agtgagacag ccattcggca tatcgctagc caatacccgt atgatacatt
    13201 caatgagaat gatttgacgc ttcgaggaaa cacgacggca gtgtctgacg agttgcaaaa
    13261 agagctgcaa gagcgtttag cagtagcagg tgtcgaagta atcgaaacac gcttgaacca
    13321 tttagcctat gcaacagaaa tcgctagtgc catgctccaa cgtcaacaag caaaagccat
    13381 tttgtctgct cgacaaacca ttgttgaagg agctgtcaca atcactcaaa tggccttaga
    13441 gcaaatcgaa gatggacaag aaatcaattt caccgatgat cggaaagtcc aattgatcaa
    13501 taatttactt gtctcaatca ttacagacaa aggaacgcaa cctgttatca atacaggcga
    13561 catgaaagac cgtaagtgac atactttttg taagatagga agaaggagtt taggacggat
    13621 tattgtcttg aactcttttt ttagttgttt taaagaaaat gacaaaattg aaagaagaat
    13681 gatagttgat tcgaaggcag aattgttcgc ttttttctat aatcagtata taaagtaaga
    13741 gagaagggga aaaagtgaaa aaacttctga tcggtctact tgttttcatc gttgtggtac
    13801 ttgcaggggt gaaaatcgca gattacgtag ttatgggtgg agaagaatac tatgtacaaa
    13861 ttacgacaga tggtacaaaa acagtttcaa aatcagactc tggacaagaa acagtcaact
    13921 atagatatat cctgcctggc tatacgaaag agggtaagga aaaagaactg gacttcaagg
    13981 gacaattgac tcgaccgctt cgtaaaggag catatttaaa aatcacctgg aacaaaaata
    14041 aaggtgtcac ttcgtatgaa gaagtcgaaa aaaaagacgt gccgaaaaca gcactggaaa
    14101 aactaattaa agggtaggga aagaaatgaa caaaatagtc gaagtacaga atgtaacaaa
    14161 agtatacgga aaaaacaatg agaaaaagac gcaagcatta agcggcatca gcttcgatgt
    14221 agaaaaaggc gaatttattg ggatcatggg cgcttctggt tctggtaagt cgacactttt
    14281 gaatatttta tccacattag ataaaccaac agatgggcat atccgaatca atcaaaaaga
    14341 tgtgaccaca ttaaaaggaa atcaactagc agattttcgt gcgaatgaga ttggctttat
    14401 tttccaagat ttcaatttat tagaaagctt gacagcacaa gaaaatatcg ctgttcctct
    14461 ttctttacaa ggcgtcagac caaaagaaat caagcaacgt gtccaaaaaa ttgcagaacg
    14521 tttatccatc tctcatattt tggaaagcta tccttctgag atttccggtg gacaaaaaca
    14581 acgggtcgcc gccgcacgtg cattgatcac acaaccaacg attctattag gggatgagcc
    14641 tacaggtgcg ctggattcca aaagtgcccg ggatctttta gatacgatgg acgaattgaa
    14701 tacaaaagat catgtatcga ttcttcttgt gacgcatgat ccgttttctg caagttattg
    14761 ccaacgtatt ttattcatca aggatggcgg tatccatcaa gaagtgaaac gtggagatca
    14821 aagccgtgat tccttctatc gagaaatact atctattctt ggaaacttgg aacagtaggg
    14881 ggagattgac tatgctttgg aaattatcac ttactgggat caagagccgt ttaagagact
    14941 acttggtgtt attctcagga ttagttatgg catctgctat tttttacatg tttgaatcaa
    15001 tggcaagcaa cgaagctttt ttgaaaagta atacgatcgt tgcggctaca gttatcattt
    15061 tccgattcgg ttctgtttta ctagggatca tcacattcgt ttatatttta tatgcaaatt
    15121 cctttttgat gacgatgcgg caaaaagact atgcaatgtt tatgatgctg ggagcaaaag
    15181 gcagaaaaat cgcacagatg attttttctg aaacattctt agtagggatc gcagctactt
    15241 tagtgggttc agctgtcggt gtaggattga caagtatcgt ccaccgatta ttagtggatc
    15301 agttagatct caatatttcg cattttacac cttttagtat tcacgggttg ttgatcactg
    15361 ttttgttttt tgctgttttg tttttattag ctgctgttgt caatgctttt tcgattgtca
    15421 aaaaaccgat tttagcattg attcgtgcgg atcagacacc aacaagaatc aagcagcata
    15481 aattcttgtt tttccttgaa gtagtactgg gaattgcttt tctaggctgc ggttactata
    15541 tgatgaaaca cgtggggacc tatcaagtct ttggtatagg ggtagcctta gttacgatcg
    15601 tattaggaac ttatttcatt ttccattctg tgatcatttt ctttttatca ttactaaaga
    15661 aatcagaaca gatttcattg aaaaaattga acaatttcac attgtcacag ttaaactttc
    15721 gtattcgtga atatacacaa atgctctcaa tggtcgctat gctgtttgcc ttggcactgg
    15781 gcgctttgac ggtaggatta ggttttaaaa atgaaattgc agacttgaca gaaaacaatt
    15841 ctggatatga tctagtgatg aataatcctc gggcagaaga tcagcaaaaa atcaaagaac
    15901 tttcaccaac actttctgct tcttatactc aaaaagaaga tgcgggaacg atttactata
    15961 atgcaagtga atttgcggct caaccattga tcatgataaa acatgaagat ggaacaccgc
    16021 cgaagatcac gaaagaaaaa gttcctgcta aaaaaatgaa tgaactgact gtacaagagg
    16081 aacttcgaag ctatgaattg ccggaacagc aagaaaaaga aatcaaggta gtcagtcaat
    16141 cagaatttga tcaactgaac ttgccacaat ccactttaca gctagttcag gtaaaagact
    16201 tccaagcaaa cctgaaacca atcgaaacac ttgttgctca aaataaaatg aacaatccat
    16261 ctttgcaaaa agtagagtac agcaaccaga aatatgaaat gtatgaagtg tataatggtc
    16321 tattctctgg ttttgaattt atgggattct tcctaggttt agctttcttg accatgcttg
    16381 ctagctgttt gatgttcaag atcttgtcag gaagcaagag tgacattgct cgttatgaaa
    16441 tgcttgaaaa gataggaaca cgacgcgggt tgcttaaaca atcgatccgt agagaagtag
    16501 gcgtgttatt cttagcacca ggaatcttag gtgtagtcca tgtcttattc ggattacaaa
    16561 tgtttggtct attcatgcaa aatccataca aagatatttg ggttccgttt acgatattct
    16621 tcgtattgta tttcatctac tatgtactga cgacatggtt atatactgga atcgtattga
    16681 aaaagagatt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa gcaaaaatag
    16741 ctcctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    16801 ttgttcggaa aaagaggttg tgacaatatt tttgtcacaa cctttttttc acggtctttt
    16861 attagaatat agtaaatttt ttctcgttac tcacggaata agctgttaag aaaacggttt
    16921 ccatgtatac tggaatcagt gcaaatgaaa gaaaagaggg tgcaaggaac atggaaatca
    16981 atgaacaaaa ttttggcgaa aactatcact tgattacgat cacgaatcaa aatgggacat
    17041 ctttgtctgt cagtgatctt ggggcacggg ttgttcattt tcaaacaata atcgataatg
    17101 aaaaacgaga gttgatctta ggttttgatg aagcaaaaga atatctagaa aaagaccctt
    17161 atatcggtgc ttcaatcgga cgtatggctg gacggattac gaatggcaca ttcaaattag
    17221 atgatgaaac gttcgaagta aaaacagacc cggacactgg tcattgttta catggtggtg
    17281 cacctagctt tgaagccaag cagtggcatt atacagtcat caatggtgaa aacgaagcat
    17341 cggtcatttt ctatcagact agcccagata tggaaaacgg atttcctgga actttagatg
    17401 tagaagtacg gtatacgctg acgaacgatg atacatggcg agtgacggtc caagggatca
    17461 gtgaccgcaa aacagtattc aatcctacaa atcatgtcta tttcaattta accggtgatc
    17521 cagcacagtc aatcgacgaa catatacttt ggttgaatag ccgattattt gctacgctca
    17581 ataaagatac aacgcctaca ggagaagtag tttcagtaag tggcacagca tttgacttcc
    17641 aacgtcctaa aaaattaggt gaggtctttg ccagtgattt tcgccaatca atgatagtgg
    17701 gcggcatcga tcatccattt ttgttgaaaa aacaatctgg tttactaaat gctgcggaat
    17761 tgacaagccc agatgaaaaa gtatcgatcc aagtaaaaac agatgcttca ggaatcgtga
    17821 tttttacagc aaattttgga gaagatggac cggaaatgcg cgggaataaa ttagtaaatc
    17881 atggcggtat cacatttgag actcaagaac tgccaggagc ggagcgactt gaaagttttg
    17941 gagatgtcac tctggaacct aaccaagtac gcgagtttgt aacggaatac aaaataaata
    18001 taaagaaata gaataaatta aaaagggagt gtgacagtgt cacatcccct ttttaattta
    18061 ttctatttct ttaaaggcag atcaagccag aatgatttgt ctaaacttaa caaggttagt
    18121 acaggaacag ttgcagcttt aactgtacga gtagttgttt ctggtatctg tcttgccgta
    18181 aaattgttcc acaaatccca gattgaagcg acacggaaga tggaagaagc gttgttgacg
    18241 cgttttatcg ttgaaaattc taatcctgtg attggcacgc catctgttgt ccatttgatt
    18301 ggcacatcga atcgcttaga catattgagg atttcagcgt ttgcttcttc agttgttgct
    18361 gcttcttttt tcggttcatc atattttgtc aggtcgtacg cttcgatgat cgcgttcaat
    18421 ttttgtgaat attggcgatc tgtagcataa cgtccttcca aatatttggt tgcttctttg
    18481 tagtcttttg tttccgactt ccaagttggt ttgtagaaat caggattgcc agtcagtcca
    18541 tttttgatca agtcggcata gtcttccaaa ctttccttgt aagaaggata tttacggaat
    18601 tgtgcgcgga tttggtagct ttgtccggaa ctatcttgtt caagtgtatt gaaggtcacg
    18661 ctgcttcctt tatatgatcc tttgatacca aaaaggttat gatttggttc gcttgataat
    18721 aagctgtttc cagatccact ttcaaggata gcttgagcca tcatgactga tgcgtagatt
    18781 ccttcttttt gcccgatttc ctgtgcatca tctgcaatct ttttcaagaa ctgatcagtt
    18841 gtttggtttt gactgaaatg gaacgagcca tctaatccat ctaaaataga tgagccgcct
    18901 gtcagcgaac ctgaagcagc aggtaaacct gctaagttta ctcggacagc aaaatcaggc
    18961 gtgaaatctg aaatagaagt tactcgaact ttatctcctg gttgaggcgc atggataaat
    19021 tgtccgttcc caatatagat acctacatgg tgagtaacac cggaagaagg tccccagaag
    19081 acgaggtcgc caggttgggc ctgacttact ggaatctgcg tacctgcata ttgctgttcg
    19141 cctgtccagc caccgatata atgaccggtt gctttcatat atacgtaata agtcaaacca
    19201 gaacagtcaa aagtatttgg accttttgct ccccatacat atggttttcc aagatgcttg
    19261 aatgcttctc gaacgattgc ttgttggaca ttcgttgtat ttgtaggcaa tacagcatca
    19321 attggtgatt ttacaacaaa atcagaagaa gcagaagatg gattctctat accgctttga
    19381 tttgctgtat ttggacgaga tgaagcattg ttcgttccgc cattcgatga accattgttt
    19441 ggatggtttg gttctctctg agtatttcca ttgttattcg atccagatgg gtttgtttga
    19501 ttcgaagaat tcccgctgtt cgaattattg ttattattgt ttgaattatt tgaaccatta
    19561 ttattgttgt tattacccac atctggtttg tttggcgttt ccggatctgg tgtactaggg
    19621 acttcttcac taggatcagt tggtttctct gttgaagtat ccggtccagc tttaaattct
    19681 gcagtaatag ataaattgtt cgtacctact gtaaaggtct ttgcatcata atttacttct
    19741 ttggcaccat taacagcgac tgaagcaatt tgttcacctt cagctggcgt aatagtgtaa
    19801 gttaccttcg tttccgctaa taaaccttct accgttccac tttccgtaat atccaaaggt
    19861 tgctgttcag gggaatccgc tgtcgaagca gtcagttgga tcttaccaat caaagaagga
    19921 gcaattgtaa gggaatatgt ctcggctggc ttttcatttt ctgatggatc agccggatca
    19981 atttcagttg gctcggtcga tcctgttgtt gagttagttg ttccagttgg gtcttctgta
    20041 aaagaatccg ttgtttcact agtagctgag ctggatgtag tcgtatctgt tgtgactgtt
    20101 tgatcagcta tttgattatc tacggcaaat acggtattgg tcgacaaaac agacggagca
    20161 atcagccaag tagcagctag caaacgtatc gtttttttat ttttcataat aaactagtcc
    20221 ttttcctttc attgattttc tgtcttattc agcattcatt agacattttt tggaacagat
    20281 aatcgtgtcg aaaagctttt tcttttcata aatctgtctg tgtattctaa ataaaacaat
    20341 ttgtaaataa gcaacgagca ttcattcgtt tttctttgac gttcatttta ttgcatagac
    20401 cacctttgtt tggggataaa caagtgggga taaaaatggt tatgcatatg taaattatac
    20461 caaagtttgg aaagtatcac aaaaatttat aaaaataatg atcctaaaaa gaaaaagact
    20521 gaaatatgtg ataaacatct catacttcag tctttttcat taactattca taagacatta
    20581 ggtcttcatc aaacccgcta acgagagtag tgatcattgc tacgactaga ctaacagcca
    20641 gtcctgctaa gtaccagaaa aaagtaccgg aaatgaatgc aggcaatgtg gtcagactac
    20701 caaatacaaa agcgatcgct ttcacttggg caaatcccat tacagctcca ccagcagctg
    20761 caccaatgca agcagcaaca aatggttttt tcaatgggat cgcaattccg taaacagttg
    20821 gttcggtcac tcccgcaagt aaaatcgaag caaaagcaga accggagatc gaacgggttt
    20881 ttgaacgttt acttcgcaga aatacaccta aagctgcacc agccattcca aaattggctg
    20941 ctcctgtcaa cggaacgatt cggtcgaacc cataagcaga gatattactg atcatgacag
    21001 ggtttacagc ccattggata ccaaatatta ccaaaatatt ccaaccacca gcgatgattg
    21061 cgccactgat cggtccgtta atatccatca tccaagtaat tgcggcagct aaaccttcgc
    21121 caaggtaaac acccgcaggc ccgaatacca ttaacgtcaa aggaaccgtg ataactaatg
    21181 aaagtaaagg tacaaatact aattgcatat tttcgcgaat atattttttg aggaattttt
    21241 caacaaaaga ataaaagaaa atacctaaaa tcgccggcat gacagtagac gtatactgca
    21301 tcagtactac aggaatcccc agaaaatcag tagctgcttt gccactgtct gaaagtaaag
    21361 aggtcagatt cggttcaatc agtgctgcag caatgacagc cgcaatatat ccattcactt
    21421 taaacgtttt ggcagcagaa aaagcaagaa agattggcag aaaataaaag acggcattcg
    21481 acgcagcaga caaaatatga taagttccgc ttgtttcact aagccagcca atcgtagtcg
    21541 ctaaaacaac cagacctttt aatataccag aaccggcaaa cagcggaatg atcggcgtaa
    21601 agatttccga tatcaaacca aatagttttt cgatcggatt tagcttttct ttcggagctg
    21661 aatgatcgat ttgacgcgtc aattccttgt aagcaggagc caatccctga tccatgatta
    21721 tttctgcata tctatcgagt aaaatgaaat acttaatgga agggatttgc tgttttaatt
    21781 tttctgcatt ggctactggc tgttttttca atttgatacg aagccgagtc gcacatggaa
    21841 ggacagaatc aatattgtct tttcctccca actgttcaat cagcatatga atctgctcgg
    21901 acagtaccat ctgttcagga ttcattgtcg tttccattag aagaccactc cactcactaa
    21961 tataatattg gcgtaaggtg tcatttcgcc tgtacgaata aatgcatgac agttattcaa
    22021 ctcttttttc atttcttcat gtgggataaa gctaatcggt gtatctggta acaataattt
    22081 gattttttcc aatatttccg ggttcattgt ctttatttct tccgccaatt caatcgattg
    22141 tacctcaagt tcttctaaaa cgttttctaa aacagataaa aaacttggaa gaccgtttga
    22201 gacagccaaa tctatttttt cagtaccaaa ggggacaggc atgcctgcat ccccgatact
    22261 taatttatca aagtgcccca tttgtgcgat tactcgtgaa atatctgaat tgattacttt
    22321 tccttttttc atgtttgttt cttcctctca ttgttaattt tagactgctg attgttctaa
    22381 agcttcgcgg taagggatag aaggttgcgc accataagac tgcacagcaa cagaagaggc
    22441 tagatttcca taaaggatcg cttctttcaa gttactaagg tctggctgaa gtgtgctgct
    22501 taatgcgcca ataaatgtat cacctgcggc tgttgtgtca acggcatcca ctttaaaagc
    22561 agggacgata ccgtgttctt tttctgtatg atagaaagcg ccggcactgc ctagtgtaat
    22621 gattactgtt ccaataccta attcatggag tttttcggct gctgcgacca aactgttatg
    22681 atctgtcact ctgattcctg taatgatttc tgtttctgtt tcatttggta taatgatatc
    22741 tgtcttttca agtagttcag taggaatcgt ttcacgagct ggtgcaggat tcaggatcgt
    22801 tgtttttcct gcgtcttttg caatagaaaa agctaggatc gtcgcatcta agctactttc
    22861 aaactgagca ataataaagt cactgtttgt aatcagttgc ttgtttttta ggacttcttg
    22921 ttcatggaaa gcattatttg cccctgcatg gatcaagata ctattttctc ctgctgcgtc
    22981 taccataatc attgcttgtc cggttgtggc gccttggatt tcagctatcc cagatgtatc
    23041 gatgttttct tttgtcagta gatctaataa ctgttgtcct tcgctgtcag ccccaacgcc
    23101 tccaataaaa gatgtccgtg cgcctgaacg tttggctgcc actgcttggt tggcaccttt
    23161 acctccgcca gcgtgaaaaa tctcatgcgc atgcatcgtt tcgcctggct tgggcatgtt
    23221 ggttaaacga agggtagtgt ccatattaat acttccaatt actgtaaccg tgttcatttt
    23281 acaaacctcc aactatctca tttattttga tgggaaaatt attttttaaa acgttttagt
    23341 aaaacgtttt aatagttcga taaaaatata gcacaccttg aaagctattt caagggtgca
    23401 gttgaaaatc ttttttctaa tcgtacaggt aatctttttt cttcccattc tttttctggg
    23461 aattggatcc gatctaacaa taacttggca gatgtttgtc ccaattcgaa gattggttgt
    23521 gcgatcgttg tcagtttcgg cttgatatac tcgcacatat ctatattatc atagccaata
    23581 atgctgtagt cttcagggat gctttttcca gcttcctcta atccgcgata taatccaaaa
    23641 gctaattcat catttaaagc aaacacacca gttgcagcag aatcaagaag ttctgctgtg
    23701 atctggtagc caccttgttt tgagaactgt gtaggtaaga ggatcagttg gtcgtgaggg
    23761 atttggtaaa catcaagagc agatttaaac ccctcaatcc gagcgtggac attttccggt
    23821 ggattttcag gatagacaag ggcgatcgtt tgatgtccta gactaagcaa gtgcatgccc
    23881 gctaggtaac caccgcgaaa atcgtctgtt cgtacggcat cactaaatcc ttcagatttt
    23941 ttctgatcca aaacaatgaa gggccggcct tgttttttca aattttcgtt gattgcatct
    24001 gtagagacag cagaagtcgc gataatgaat ccatccacac ctcgacgtgt gagctcggct
    24061 agatattcga tttctttttg atgatcagaa tcagcattac ataatatcgt gacgaaattc
    24121 tgcttataca agatatcctc aattcctcgc atcagtgtac tgaagaatgg attcgtgata
    24181 tcaggaacga gtacaccaat tgttttcgtt tccttggtaa tcatttgtct agcaaaatag
    24241 tcaggctgat agcctaattc ttctttggca gctaaaactt tctgaacagt tttcggactg
    24301 aatttctccg tatttccatt taatatcaag gagacagttg tgattgaaac gccggaaagt
    24361 ttagcgactt ctttgattgt gatctttttt ttcaccatgt tcatactcct atctaaatcc
    24421 tgtggttatt ttcccatgaa atcaaaaaga agtaaatgaa aatcacgtgt gccagtactt
    24481 atttgctttg ttggatagct tcgattattt gtttcgttga gcgtactcgt gcagttttcg
    24541 gaaatatctt ttttatgaac aattgatggg attctttgtc acgatcactg caagcatctt
    24601 cagcaagcac gatatggtac ccgtgttgaa aggcatctaa tgcagtagca tatacaccgt
    24661 ttgttgtcgc tacaccgctt aaaatgatag tttcgattcc acgccggcga agttgaagat
    24721 caagagaagt accaaaaaaa gcacttggat tgtattttgt gatagtcaac atattatctg
    24781 tatctttcaa agaatccttc aaaagtaaat cagtgaattc tggaggtaca gaagcagtca
    24841 ttccacccat atcagtttgt tgactcaaat aagggaaagt ggagatgtct acgttcacta
    24901 aaactttcaa ggcagcggta cgtttcaatg ttcttactaa caaatcattg tttgtcagaa
    24961 tagatgctgc agtgtgtgga gagagtgtac cgttatttac gatctctttt tgcatgtcga
    25021 tcgcgacaaa agcagttttt gataaatcaa aaatcatatt ttcctttaaa tcattcatga
    25081 tgttccttct ttcaaataac aatgtttttt atgtaagtaa atcataaatc gtttatttgt
    25141 caactaaatt aaaggattcc tttctttagt ctagagaaat tcagtggtaa aataaataag
    25201 tgtgagaaaa cgcattcgat gcatagatat agcgagaaat atcgtgaaaa tggaggaatc
    25261 agagtgaagt atgtcgaagt aaaaaatgaa tacaaacgat acaaaatggg agaaacgacc
    25321 attacagcaa atgacgatat ttctttttca attgaaaaag gcgaattagt gattatactt
    25381 ggtccaagtg gagcaggaaa gtcaacgatt cttaatattt taggtgggat ggatacacct
    25441 gatgaaggtc agatcattat tgatgacaca gatattgcgc aattttcaga taaacagctt
    25501 actgcttacc gacgaacaga tgtagggttc gttttccaat tctacaatct cgttcctaat
    25561 ctaacagcaa aagaaaatgt ggaattggca acggaagttt ctcctgatgc gcttgatcct
    25621 gtagaggtat tgaggcaagt gggcctagct catcgattga ataactttcc gtcccaatta
    25681 tcaggaggcg aacagcagag ggtttcaatt gctcgtgcac tagctaaaaa tcccaaactt
    25741 cttttatgtg atgaaccgac aggcgcgctg gattttgaaa cgggcaagca agtattgaaa
    25801 ttattgcaaa acgctagcag acaacacggc aataccgttt tgatcattac acataattct
    25861 gctttagcgc ccatcgctga tagagtcatt cacattaatg atgctaaagt aaggtctgtt
    25921 gaattaaatg atcatccttc atccattgat gagattgtat ggtaggggga ttttatgaaa
    25981 acaagaacat atcttaaagc aggttttcgc gaaattcggc aatctaaagg acgattcatt
    26041 gcaatcatcc ttattgtcat gctaggaaca cttttatttg taggtgtcaa aactgctgga
    26101 cctgtcatgc aaaaaacgat ggatcactat gttggtacag caggactttc tgatttgcaa
    26161 atcgtttcta ctggtggctt gacacaaaaa gatattgctc aagcagaaaa gatcccagat
    26221 gcaaaagtag aaacaggaaa acagatctat tattccaata ctggcaaaaa cgaagtgatt
    26281 caaatatttt cttataacaa aaataacaag cagaacaagc tacaattaac tgatggaaaa
    26341 ctcccggaaa aaccaaacca gcttgtattg gatgaaaaag cggaagatga agggtataaa
    26401 atcggtgata tttatcagat tgattcagat gaattgaaag aaaaagagta taccatcacg
    26461 ggctttgtcc gttctccatt gttcatcaac aatttggaga gaggatacgc gaacgtcgga
    26521 aatggtagcg tcgattattt tgtctattta cctgaatccg tttttaaaac agatatttat
    26581 tctgtcttat atcttgattt tacaaacgta aagaatttga atacgtacag caaagcttac
    26641 aaagataaaa tggagcaaaa tcaagaaaag gcagaaaagt atttacaaga tcgacccgaa
    26701 gaacgcttag aagagctgaa aaaatcggcc aacgaagaat tagaaccggc acagcaaaaa
    26761 atagcggatg gcaaagcaca aacaacgcaa gcaagaacac ggttagaaga tgctaaaaaa
    26821 cagttagcgc aacagtcagc aactatccag cagcttcctg aagcgcaacg aacaatcgct
    26881 gttcaaacat tatctcaaca agaagctcaa cttgctgaac aagaaaggca attagttgag
    26941 aaagaacgtg aactggcctc tgcagaggaa gaattgaatc gaaatgcaga aaaaattgaa
    27001 aatttaacta aaccatcata cctatacaat gaacgttcag aaaatgtcgg ttttcaagaa
    27061 tttggtgatc tgtctgatcg tatcgctgcg attgccaatg tttttcctgt tttctttttc
    27121 tttattgccg cattgatcac ctttacgaca atgacacgta tggtagaaga aaaccgcagg
    27181 gaaatcggga cattaaaggc gttaggttat acgaaagttg aaatcgcaaa aaaatatgca
    27241 atctatgcaa gtctagcctc agggataggt attgttttag gaacgatatt aggaacgaat
    27301 cttttgccaa ggatcatttt tgagctgtct aacgagcggt atgatattgg gagtgcgctt
    27361 attttttatg actggccgcc gattttacaa gcagctgccg cattcttcat tgccgcattt
    27421 ggcgctgcaa tgatcgtttt attcaaagat ttaagagaac ggcctgctgc attgcttcaa
    27481 ccgaaagcac cgaagccagg gaaacgcatt tttcttgaat acatcactcc gctgtggtca
    27541 cgccttagct ttaatcaaaa aatcagttac cgaaatctct tccgatataa gtcaagaatg
    27601 cttatggcga ttatcggaat tgctggatgt gccgggctga tggttgcagg cgtgggattg
    27661 aaagattctc taagttctgt ttcagccaaa caatttggac cgatcattga ctatcaagca
    27721 atcgttacat tggattcatc cacggaagat agtcgtcaaa aagcagcgga tatgctgcag
    27781 aagcaagaaa aagtaaatga gatcctagct attcatactc aaaccgtaga attgagaaaa
    27841 aaaggacatg cttcgcaaag tctgacagtg atcgtaccaa gtaaaacagc tgatttggat
    27901 cgttttatcc atttaactga tcttgaagag aacccacgct ccttgcctaa aacaggaatc
    27961 gctattacgc aaaaagtagc agaactattt gacttatcta caggagatca tatttcgctt
    28021 tatgatgaag atcaaaaaga atggaaagtc gaagtaaaag aaatcgtgaa aaactatcta
    28081 ggtagcttta tttatatgtc ccctgaatat tatgcttcag ttactgatca atcgatgatg
    28141 gataatgcat ttttgctgcg tacagataag atgacaacaa aagaagaaga aatgctttcc
    28201 gagaaactgc ttgccacaga cgctgtgaca aatacgtctt tcatgtccaa acaaatcgaa
    28261 acgcaagaag aatcaatggc gaacttagac tcggttgtct taatctttgt cgtattatca
    28321 ggactgctgg cttttatcgt cttgtataat ctaactaata tcaatatttc cgagcgtgta
    28381 cgcgaattat ctacaataaa agtccttgga ttttttgata aagaagtcac gatgtacatt
    28441 gttcgagaaa atatcatctt tactctttta ggaatcatcg gaggatttgg tgtaggatat
    28501 atattgactg acttcatctt gcaacaagcg tctatggaga atgtcatctt tccattagtg
    28561 atcacatggt gggcttatgt cttatcagca ggattaacta tcgtatttac ggttattgtg
    28621 atgattgtta cccatttcaa attaaagcat atcgatatga ttgatgcgtt gaaatcgaat
    28681 gaataaaaac agaggttgtg aaatcagcgg tttgacctga gtattagaga aacaaacggc
    28741 aaaatagttt ttcatatttt gccgtttttt gagctaatac cgaaggacag ctgattgaac
    28801 accatgaatc agaggttgta aaaaagccgt cctgcatcga gcaaaaagaa tactcaaata
    28861 aaattagctc ctcatgtttt acatttgaga agactgattg ccgtagataa aagcttttga
    28921 acaccattta tagaagaaac aagaatattt tgctatttta aacaagagga tagacaaata
    28981 catgtctgat ctcttgtttt ttattgggca acagacctct tagaatgtat aaagataaat
    29041 tttacaaacg ctttcggttc agttataata ataaatgata acttgtatat ataagttgtg
    29101 gtaggtaata aatcagttga atggaggtaa atatccatga catttaaaga caaagtcatt
    29161 taccaaatct atcccaaatc ttacaaagac agtaatggtg atggaattgg agatttaaaa
    29221 ggaatcaaag aaaaactccc gtatctgaat gatctaggca tcgacatgat ttggctaaac
    29281 cctatttatc caagccctca aaaagacaac ggttatgata tctctgatta cacagccatc
    29341 gatcctatct ttggtacgat ggaggaattc gaacagctaa tagccgaagc aaagaaatac
    29401 cggatagaaa tcatgctcga tatggtgctg aatcacgttt cgacagaaca tgaatggttc
    29461 aaaaaagcac tagcagggga ccagcattat caggacttct tcattttaag agaagagcca
    29521 acggattggg tatcaaaatt tggtggaagt gcttggtcgc catttggaga tacggggaaa
    29581 tattatttgc acttatatga tcggacacaa gctgacttga actggcggaa ccaagcagta
    29641 agagaggaac tattcaaagt tgttcgtttt tggatggaaa aaggggtaaa aggattccgt
    29701 ttcgatgtta tcaatgttat cggtaaagac gaacaattga aaaataataa agaaaatgat
    29761 ggcaaacctg aatatacgga caaaccaatc acccatacgt atctaaaaga attgaaccaa
    29821 gcaacgtttg ggacggattc cactatcatc acagtcggtg aaatgagctc tacgacgatc
    29881 gaaaattgta tcttgtatac agaacctagt agagaagaat tatcaatggt ctttaatttc
    29941 catcatttga aagtcgatta tgaaaatggg aataaatggt ctactcctcc atttgatttt
    30001 gcagaattaa agcgactatt tcatacatgg ggagaaaaga tgagtgaggg taacggctgg
    30061 aatgctctat tttttaataa tcatgatcag ccacgtgcgc tcaatcgatt tgtagctatc
    30121 gatcagtatc gaaaacaagg agcagagatg ctagcaacca tgatccattt gaaccgtggg
    30181 acgccctata tttatatggg agaagaaatc ggcatgatcg acccagacta ttcatcgatt
    30241 tccgactata ttgacgtaga aagtttgaat gcttataagt tactccttga acaaggattc
    30301 acagaaaaag aagcgtttcg tcggatcaaa gcaaaatctc gcgataactc tcgaacaccc
    30361 atgcaatggt cagacacaga acaagcagga tttactacag ggaaaccttg gttaaaagtt
    30421 ggaggcaagc tagaagagat taatgtagaa aaagaactat cttctgaaga ttctattttg
    30481 tcctattaca agaaattgat tcggctgaga aaaacatatc caatcgttgc acaaggagat
    30541 tatcatgctt atgaagcaaa tcatccggaa gtttatggct ttcttcgcca atttgaagga
    30601 cagcagctgc ttgtattaac aaatttctat gataaagata caaggatcgc catcccagaa
    30661 ggattcacag aagccaatat tcttatcgat aattattccg atactgttct tcatcaaagt
    30721 ctggagctaa aaccatacca ggcaattgct ttgctgaaaa attaaaacaa aaacacaaaa
    30781 cgtcgttcct acgagctatt ctcgtaagat cgacgttttg tgttttattt aaaataatct
    30841 tcttcagaaa atgaagagtt ttcatactct cctttatcgc cataaaattt attgtagcgt
    30901 tgtttgaatt tacggaaaat aaaagtaacg agtaatttga ctaacgtata gataggaata
    30961 ccaaaaataa cgcccacaat tcccattaga tcacccatga caagtaaaac gattaagatc
    31021 gtaatcggat ggatctgtaa acgatcaccc attactcgag gaatcaccaa gtcaccgtgg
    31081 agaagttgta cggcaaacca gacaatgaca aatttgacag ccataaaagt agagtcttgg
    31141 aatgcaatga tcaaacctgg aaagaaggcg ataaacggcc caatatatgg aatgatacat
    31201 aacaacccag cagaaatagc taaaacacta gcaaattcta atccgatcag taagaaacaa
    31261 acccagtaga ctgctccaag gataaatgaa gcaatgattt gcccctttag aaatgaaccc
    31321 aattgttgat tagcaatctt tgtcaaattt ttaaaatctg tacgaaatgc tggtgggaca
    31381 atcgctaaaa ctgactgata aaatttttga ggatctctca aaaggaaaaa agcgacaatt
    31441 ggaccagtaa ataaagtaat aaaaatagct gtaacagttg aaaaaatgtt gctgatccgt
    31501 tgtgcgccac tttcccaata atttccaata aatccagcta tatcagacgt cacgtcgtca
    31561 atagaagcga aaaattgatt aaatgaatcg gcaaaaggtg ttttctccaa aaattgtcgg
    31621 agactctctt gaaaatcttg aaacaaatct ggcaaactac tgatcaatgt ctttgcctgt
    31681 tcttcaagaa aaggaataag ccatatgctt ccaagcccca ataaaaataa aataatcaca
    31741 taaacgataa tgactgacaa gttcctcgaa aggtgcttgc tcaaacgttt gatgactggt
    31801 tccaaaagat aatacaagat cagtgcaaac aataatgggg gcaagatagt agcgataatg
    31861 attccgacag gcttgaatat aaacgagatc tgatggaaaa aataaatgat cagcgcaagt
    31921 aagataacta ttcctaaaac atagtaagag gtttttcctc ctaaaaagcg gatcaaacga
    31981 ttcgttgtat ctgtttcttt tttcactctt tttcctcctt tatctataac ttcattatag
    32041 acaaaagcgg gtataaaagg aaaatgatag aggttgattg caaaaatatc gtcaatcatt
    32101 catataaact ttctcatcaa ttaaaataag aaaacgcttg acagttcttt taaccatatg
    32161 ataaattaaa tcctgttgct aaaacgtaac tattacggtt tgcgaaaagc gaatcggaac
    32221 attcaaatga aagaagatga gaagaggagt gtcataaatg aaacgattga taggtatatt
    32281 ggccatgctg gttttagcag gtatgttaac tgcttgcggg gcaagtggga aagccgagga
    32341 cagtaaagaa aaattgagcg tgatgacgac cttttatccc atgtatgatt ttacaaaagc
    32401 aatcgtcggg gatgaaggag aagtagagtt attgattcca gcagggactg attcccacga
    32461 ttatgaacct tctgctaaag acatggcaaa gatccaagat acagatatct ttgtttataa
    32521 tgatgaaaac atggaaacat gggtccccgc aatccaaaaa acattacaag aaggcaatgt
    32581 ccatacgata aaagcgacag aaggtatgct tttacttccg ggaagtgaag aaggacatga
    32641 tcatgaccat gaacacgggg aagaaggcca tacacatgag ctagacccac atgtatggct
    32701 agctccaagt ttagcaatca agcaggtagc aaacatacgt gatcagctga ttgaagctta
    32761 cccagaaaaa caggaagtat ggacaaaaaa tgcagcagcc tataccgaga aacttcaagc
    32821 actccatcag ctttatcaag aaacctttaa gcaagcaaaa caaagatctt ttgtcacgca
    32881 acatgctgca ttcaactatc tagcgttaga atatggattg aatcaagtat cgattgctgg
    32941 tctttcttct agtgaagaac cgtctgcggc gagaattgct gaactgaaac actttgttaa
    33001 agaacatgga atcaactata tttattttga agaaaacgcc aaagatagta ttgccagaac
    33061 gttagcaaac gaagcaggtg tatctttaga agtattgaat cctttggaag gattgacgaa
    33121 tgaacaaata gaaaatggag aaaactatct ttctatcatg gaagcaaacc tcgaagcgtt
    33181 gaaaaaaacg acagagacag aaaacccgct agaagatact ttgaccccca aaaaagaaaa
    33241 gagcgtctac aacggttatt ttgaagacga ggatgtacaa gatcgctctc tttcagattg
    33301 ggcaggaact tggcaaacag tagatacgta tgtagaagat gggacttttg atcctgtttt
    33361 tgaatataaa gaaaaattga atcaggataa gacagcacaa gaatataaag aatattatac
    33421 gaagggctat caaacagata ttgcttcaat caagatcagt gagaaagaga tggcatttac
    33481 gtttaagaat ggaacaacga aacgttcaaa ttatcgttat gtagggaaag aaatcttgac
    33541 ctacgaggca ggaaaccgtg gcgtaaggta tctattcgaa gcagaagata aggaaagtgg
    33601 cgcttttaga tatattcaat tcagtgatca cacgattgca ccggctgact ccgaacattt
    33661 tcatttatat gcaggaaatg aaagtcaaga agtattgctg aaagaaatgg agaattggcc
    33721 aacattttat ccagatacaa tggatggacg tgaaattgca caggaaatgc ttttgcactg
    33781 ataggcaaaa aagaaccgaa gggtttggat cggctggtca tttagtcggt ccaaactatt
    33841 cggttctttt tatgaaatca atttctttaa tccacggata gaggctggga tggtctcgcc
    33901 gtttgccaac gtgacttctc tggttttttt attaatcgac cgcacatttt gtgtattgat
    33961 aacataggat ttatgacaac ggataaaatt ccgatgcatt ttctcgattt ctttgatctt
    34021 ctcatagaat tgtaattggg catgagtcaa atggacttcc agtttatgtg gcgctcctag
    34081 cgaagtagaa aaatattgga tgtctttgat cggtaaaaat ttgatctcat cattaaactc
    34141 tattttgaag atatcttttt ggatttggga catttgaaaa tcttcgaaaa ttttgctcag
    34201 gatatgctcg acattttctt ttaaatcata aagatcttct ttgacgatat aatcagtcgg
    34261 ttcgatgttg ctttttaaga ttttcatcga catttccgta tgggaagtca catagattat
    34321 ttttcctaaa ggatctaatt cacggatcaa tttaccgatc gctacaccat ccatttcact
    34381 gtcttctagt tcgatatcta aaaagtaaat accaaaagaa gaagttctga tcttggctgc
    34441 attgagaagt tcttccggat cagaggtaga aatatggaca taactgtcta agttttcaat
    34501 catgattcta tttttgatca ccgttgataa aacttctaat tgttttggat cgtcttcaca
    34561 aatataaatg ggaatcatat cattactcct ttattttatt tgtatataaa tcgtttgaga
    34621 aaaacgctgg ttttcaattg atgtttcaag gaaaagataa ggattggtct ttgtcaattc
    34681 atccaagata aacaagccaa ggccttcgtt ttttggattt gtttttgttg agaacccttt
    34741 ttgtttcaac tggtttaacg gtaatgtatt tgttgttgta ttgatcacac gaatcatcac
    34801 ggaatgattc ttcttgagta tggtcaattc gatttttttc ttctttgcag cactagctcc
    34861 ttcaatcgca ttgtccaaga taataccaag catacgtaca aggtttaccg tatgtttcgt
    34921 atccagttcg attggctcag gaaaatgaag ctggacgatc aacccttttt cctgtgccat
    34981 catgagtttt aatgacagta aactacggat ctcaggaact ttcatattat ccaacatact
    35041 tagattggcc ggaaaaaggt tcatcatctg gcgtgtaggt aatatcgttt gatcataata
    35101 agaccgtaat ccttctaagt cgccttctga aatatagctg tgaagagaaa aaagcagatt
    35161 ttggtaatca tgtttgaatt ctctcaagtc ttgatagttt ttttctagat ctttgatata
    35221 atcctgttgc atcctgactt gttgctctaa taactgatca gctaatttct tattagagat
    35281 tttccaataa taaaccaaga tgatcaggcc aacacacaga tatagaatga aaaaactcgt
    35341 attcaaagct aagacatcag gtgtttcacc agaaaaacgg gtataaaaaa taatgatata
    35401 gtatgtaaat aatatcaatg tcccgatcgt accgatcata cggcggtcca cagtgtgaaa
    35461 ccaagtctct aacgattttt ttaaacaaaa agctaataaa gcagataaaa aagcggataa
    35521 gacaatgtga aaatactgca atgaatctcc tggttcaata accgaagatt tcaaaacaaa
    35581 aaaatcgccg atgcttgtta agtgatctcc taaaagcgac gtcaataacc caaaaggaag
    35641 cgcgtaaacc aattgttgac gcgaagtcga aaagaagaaa aaagtaagca tcagcattcc
    35701 tatagcaatt agagcagtaa aaatccctaa aatcgaatag acccatccaa tcaaaataca
    35761 agaaatcaga atgactcccg caaacagata tttgtatcgg atgtgatggt caatcaataa
    35821 gatacaaatt gtgtaaatca aatattgcaa cgctatggac cataaaaaca taaagtacag
    35881 ctcccattga tttttaccaa attatagcac aattggcgta ttattttttt aatttttcag
    35941 ggattttgaa ttcagaactc ataccaaagc agcagccaga tacaaaaaag tcagataagt
    36001 tagttaattg tttaatgatt gtttttttca tgaaagattc ctccaaaatt catcaagtag
    36061 tttgtaatag gaagcaacgt agctatttct aaaacaatcc caagtagcaa ataggtagaa
    36121 aagtaagaag ctgagaaaaa taacgtaatg agagaaaaaa ttagaaacat gctgatcgcc
    36181 ttctttaata atgggggacg tttctcgggt tcaatgggat tgattgctgt acctcttgat
    36241 ccatagcgaa gcagtaacaa aaagcagctg attaacaaaa agaccatcat tgggaaggag
    36301 atagtcaatt gcagatgttt catcaccaag ggaacgcccc ataaataaag tagtgtccac
    36361 agcagacaaa cgaattcaga gtcggaatgc aatccataag catatctgcg gatgcagata
    36421 taagaaagaa aggtgattcc tgtttccgcc catgagcctg tcaccagaga aaagactaga
    36481 acgaccgcta gttttccaag attattcaat aaaacttcta agacaaattt tacttggaca
    36541 tacaccatat cttcttccga tttgtctccg ctgaataagg cttctaataa tttgttcaca
    36601 actttatctt tcatctcata cgtacctcca ccacaaattt tatgctaaaa aaaataaaaa
    36661 acgtcttttg tttcataaaa agtggttttg acatcaaaac cgtcatgatc tcctaaaaaa
    36721 atcttcgttt tccaagaacc ttaccaaaaa aagtgacagt tttgatacga aaagtacttt
    36781 ttatgacaag tttgtgcttt ttcgagtgat ttatgttaaa aaattactga ccttataaaa
    36841 ggtgatggag ggattcttat ggaaaatgaa caattgtttg aagtcggcga aacatatgca
    36901 tttaaatctg atttatttga atcaccaata aaaggaaaaa tagaaaaaat ctatgaaaat
    36961 agtgtgatga tacgtgtcat gaaatgcgca gtagaagaca atgataccgc tcataatctg
    37021 cactggcagt tagttgtacg caaatcaagt acaatagaag aataaaaaca gataaaatgt
    37081 tgagagcata aagaggttgt gagtctcccg tttagctctg agttacaagg aataatgtta
    37141 aaaaacagct tttcatgttt tatgaatatt gccttgtgac cgaggtgttg gcaggagaat
    37201 actgtttatt tgaaaaaagg gctgcgacaa gacttttgtc acagccttta ttttcatgaa
    37261 taggagttac ttttggctac ttttagagaa gtagctttta aggagcagat aaagtcaagg
    37321 cttttatttc tataaatgaa gtagtaaaat gaagaacaga ttttattaca ggaaggatgc
    37381 cgataattgt gatcattgtt gcaggaacga tcggtgcagg gaaaagcaca ctaactgaaa
    37441 tgcttgccca agatttagaa acaaaacctt tttatgagaa cgtggaagat aatgaagtat
    37501 tgccgctatt ttattctaat ccagaaaagt atactttttt attacagatt ttctttttga
    37561 acaaacgctt tttggcaatc aaagatgctt tcagccatga tgacaacgta ctagaccgct
    37621 ccatctacga agatagtatg ctgtttcatt tgaatgcaga tttagggcgt gtttctgaag
    37681 tagaagtaaa gcagtatgaa ggtcttttag aaacgatgct gaaggagtta gaagaaattt
    37741 cgccacaaaa gaaaccagat ctactggttt atattcgtgt atcctttgaa actatgttag
    37801 cgagaatcaa aaaaagaggc cgggaatatg aacagctaga acaagatcct gaactctatt
    37861 cctactataa agaactcaat cgacgatacg aagagtggta tgaacagttt gatatatgtc
    37921 ccaaaatagt gatcgatggg gacaaatatg atttcgtagc cgatccggca tgtggagaac
    37981 aaatcgtaca agaaatcaag atgcgggcaa agaaaatgac agaagaggca gatgcccagt
    38041 cgatccgcta cactacaaat aacccgaaag caaagggaat ctttacaact aaataatata
    38101 aaaactaagc aaattgctca ctcgcagata tttctctgtg agcagcatct tgcttagttt
    38161 ttttgattca ttgagatcaa atagtgaacc gattcgtatg ataaaattgc cataaatgaa
    38221 atgtctgagt gagggccatg atcaaaaata aaatggaaac gatcaaaatc gaatgggcgc
    38281 caaacagatg atgacagaaa acaaggacaa tagctcctgc gataaaggca agaacgacac
    38341 ttagatagtg gaaaaatcga tcaaaatctg ctttttcttt agaaatacaa taatcatata
    38401 agttcgttgc tacccctttt aaatttccag ttgtaaataa attagtgtag gcacgcccgt
    38461 tgattttatc aaaagctacc cactggatcg atgcagtaaa agcaatcaaa atcgttacta
    38521 atgcgggatg ctcatggacg ggtgtcaaac taacaaagaa aaaaatcact gcttcataat
    38581 atagaacaaa caagcgccag aaatgttttt ctttttttct aaaataatca attagatact
    38641 ttgttagaag aatccctaag aaaaagaaaa gggtagacaa tactttttta ccaacactta
    38701 gccattctcc gtcaaatgct tggatgatcg atagaatgat attgccagtt tgagcagagg
    38761 caaaagcacc atattggata taagtataac tgtccatggc tcctccgata aatgtcagca
    38821 aaagtcctat tgtacgcccc tcgtgaaagg aagtctgcat ttttttcatc accttttatg
    38881 aatatttttg tcttccttat cttagcataa acgattgaaa aaaaggaaaa attttcagaa
    38941 aactaaaatc atatcgcttc ccattttcat ttttagaaag gtctgttagt ataaagaaaa
    39001 aatagacaac gaaactttaa ataaccaact tgaaataaac aaattcaaag aagaaagatg
    39061 ggaaggaaat caatcagttg aatgaaaaag aaattctccg attacaaaaa gaattgaaac
    39121 gctgggagaa caagtataaa aagcttcaac aagtctttcg gctacaagaa gaaatcattg
    39181 ctacctatga agcggcttat ggcgaagcac ccggacttat tgaggaagaa gtacaagtaa
    39241 aagaaagtga aggaaacagc tctttcttta aaatgatgga gaaacgttcc tataatcgct
    39301 tcaatcaaga acaaaaagca gctatcacat atgatatgaa gaaacatctt cgaatcatag
    39361 caggagctgg gagcggcaaa acgcaaacaa tctgtgcaaa agctgtttat ctgatgacgc
    39421 aaaaacaagt agacgaagaa cgcatactaa tgatcacttt tacaaggaac gctgcaaatg
    39481 aactaaaaaa acgagtagat aatttcagcc agagaaaaac cgctgtccat atcggaacat
    39541 tccatagtat cttttttagg atatacaatg aaatttgtcg aaaatttcct gaagtagcaa
    39601 tgcaaggaat acaaggagac ttttcaaagg atactgcaca aaaagtgaat gctgttttgc
    39661 aacagctgat acgtaaatat aatctttata tatttgatca atatggagaa aaaacgattg
    39721 cttctcggtt agactattgg cagaatatga acctttcagt agaagaaatg gttcagttag
    39781 ttaaagaaaa atatgattcc attgataaga atgcacgaca gccgatatca gaacgactgt
    39841 atggattgct cacggaactc caagaacaaa aaagaagcca gcaattgcta gagttcaatg
    39901 atgtactcca aaatctgaaa agcgctttgg agaatgagga gatccagcat tatatcggcc
    39961 aaaaatatga ttatctattc attgatgaat ttcaagatac taatccactt caatgggaaa
    40021 tcgtgaaacg gatgacaaag gaaaacagga tcaagttgat tgttgttgga gatgatgatc
    40081 agagtatcta tggattcaga ggatcagagc cagcatacat caagaacttt gaaaaagaat
    40141 atcctaccaa aactcttttc ttattaacta attatcgttc tcgggcagct attgtccaag
    40201 gagcaaatag attgatcagc tataataaaa atgatcggat accaaaggcg atgattccag
    40261 cacaaaaaga agcaggtgtt atggaagcct atttgttttc tgatactaaa gcagaaagtc
    40321 agtggctaat caaacaaatt cagacattta ttcaaaaaaa tgggaaatat aaggaatcaa
    40381 tcatattata ccgatcagct tcacaaacgc agcagcttgt ccaatctctg ctgaaaacga
    40441 atattccttt tgtactggaa gcagatagtc catatgaagg tatctttggt atcaagtctt
    40501 tccaacattt ctatcaaaaa attgttcatt ggcaaacagc actgaatctg caaaagaaaa
    40561 atcaggccta ccagcaattg ctccggcagc tgatggcaga ctgctacctg aaaaaaagtg
    40621 aatgcgatca atacttttct cctaaatatc aaaatatagc cgttgcagat tatattttgt
    40681 cgataagccc caatttaaaa tcgaaatctg cagaaatccg agagtttgag gaagcgttgc
    40741 gtaaaacaga ccagcatcaa gaaatctatt cgcttgttca aagctatctt catctgccac
    40801 gtatagctaa ggaaatcgac cagagtgatc aagaatggat acgagaagaa gtaaagcagc
    40861 atgctacttt cagaagactc caagccttat cgcaagaaac ccaagcgaca gctaaagaat
    40921 taaaaacccg cttgattgct tacagacaag gaaaacttga tgcactatgt atccagagca
    40981 ttcacaaaag taaaggtctt agttaccgaa atgtcttttt gattggttgt aacgaaggaa
    41041 gtttgcctta taacggagcg acagaaaaga aagtaatcga ctttaatgaa ataaaagcag
    41101 aaccagtaac gacgatagaa gaagaacgaa gactatttta tgttgcgatg actagagcaa
    41161 gaaatcgtct gtatctatgt gtacctcaaa tgaaaaattc aagaaaattg aaagtatcga
    41221 gatttatcaa agaaaccggg agtcgtgtga aaaaaggaaa gtgatagaag agagggtgtg
    41281 acagaagtgg tcagtttcaa gaaagaagaa ggaatgtacg aaaattgctt ctcaaatttt
    41341 tgtgaaattt cagcttattt tcgaagaagc tatttctgga acacagttta ttcggaaaag
    41401 ggaggtgtga caagaatttt gtcacacctc ccttatt
//
LOCUS       DAMEET010000012        39628 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.012, whole genome shotgun sequence.
ACCESSION   DAMEET010000012 DAMEET010000000
VERSION     DAMEET010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 39628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.012"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            4..816
                     /locus_tag="PB780_000571"
     CDS             4..816
                     /locus_tag="PB780_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HDE0732646.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="PB780_000572"
     CDS             813..1613
                     /locus_tag="PB780_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDE0732647.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="PB780_000573"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="PB780_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDE0732648.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="PB780_000574"
     CDS             3110..4819
                     /locus_tag="PB780_000574"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDE0732649.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9383
                     /locus_tag="PB780_000575"
     CDS             5031..9383
                     /locus_tag="PB780_000575"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HDE0732650.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9527..10000
                     /gene="rimP"
                     /locus_tag="PB780_000576"
     CDS             9527..10000
                     /gene="rimP"
                     /locus_tag="PB780_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDE0732651.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10024..11199
                     /gene="nusA"
                     /locus_tag="PB780_000577"
     CDS             10024..11199
                     /gene="nusA"
                     /locus_tag="PB780_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDE0732652.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNSKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11221..11514
                     /locus_tag="PB780_000578"
     CDS             11221..11514
                     /locus_tag="PB780_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HDE0732653.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11511..11822
                     /locus_tag="PB780_000579"
     CDS             11511..11822
                     /locus_tag="PB780_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HDE0732654.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11835..14141
                     /gene="infB"
                     /locus_tag="PB780_000580"
     CDS             11835..14141
                     /gene="infB"
                     /locus_tag="PB780_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDE0732655.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14168..14515
                     /gene="rbfA"
                     /locus_tag="PB780_000581"
     CDS             14168..14515
                     /gene="rbfA"
                     /locus_tag="PB780_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDE0732656.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14602..16278)
                     /locus_tag="PB780_000582"
     CDS             complement(14602..16278)
                     /locus_tag="PB780_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HDE0732657.1"
                     /translation="MLQQSNNTITSIYPIGNQTEELTALYCRLSQDDKQEGDSNSIIN
                     QKKILKRYAIEHGYQPYVFFVDDGFSGTNFNRPDFQRMIAEVEAGRIKRVIVKDMSRL
                     GRDYLQVGMYTEIMFPNMDVHFIAVNDGVDSHVGENEFTPFRNIINEWYAKDTSKKIR
                     AVKRSKGMAGEHIGSHAPYGYMKNPDNKKEWLIDEEAAEVVREIFRLCVNGYGPTRIA
                     NILTERKILCPTYYALEHGEKPRTVLPPDKYLWSATVVSHILERMDYLGHTVNFKTHV
                     KSYKNRKKIDNSPEQWRIFENTHEAIIDKETFEIVQKIRSGKRRPTKMGDMPMFSGLL
                     YCADCGSKMTFHRQMAQSAEKHNFVCSNYRHNSKSCTMHYIRNVVVEQIVLENLKEVI
                     RYVSDYEDEFVQMVMDTDMRQRNKELSQKKKRLTEIHSRIQELDKIFQRIYEDNISGK
                     LSDERFMKLSKGYEEEQHTLQEEQTSLEKELQKEEKQSVDVKQFLSVVRKYTNLTELT
                     PEIVHEFIDKIIVHAPDKSSGKRLQEIEIIYNHIGVFDHSKVTLWKGKAV"
     gene            complement(16373..16525)
                     /locus_tag="PB780_000583"
     CDS             complement(16373..16525)
                     /locus_tag="PB780_000583"
                     /inference="COORDINATES: protein motif:HMM:NF025565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-encoded TnpW family protein"
                     /protein_id="HDE0732658.1"
                     /translation="MENTKNPAPEMIREYQIGNTCYVVKSRSKEQAQEDAVTKVKRLI
                     RNDLKQ"
     gene            complement(16982..17221)
                     /locus_tag="PB780_000584"
     CDS             complement(16982..17221)
                     /locus_tag="PB780_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015552516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDE0732659.1"
                     /translation="MTAKHPMIPFPVIVRAADGDIEAVNQIVRHYSGFIASRSMRPMK
                     DEYGNTHMVVDETLRRRMETRLIAKILSFEIREPK"
     gene            complement(17218..17637)
                     /locus_tag="PB780_000585"
     CDS             complement(17218..17637)
                     /locus_tag="PB780_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDE0732660.1"
                     /translation="MKPSEFQTTIENQFDYICKVAMEDERKDYLKALSRQCKRETLFC
                     DMDDYTVNLFSSEDTYPSYFHTFEMDGFTVRIENSLLAEALENLDGKKRDVILRYYFL
                     GFDDTEISKILEVNRSTIQRRRHAGLEFIKKFMEDKA"
     gene            complement(18093..18866)
                     /locus_tag="PB780_000586"
     CDS             complement(18093..18866)
                     /locus_tag="PB780_000586"
                     /inference="COORDINATES: protein motif:HMM:NF024141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDE0732661.1"
                     /translation="MRNIIKTEFLKLKRYSVIKAGIIMTTLSPLLSLFYSTANGGQTW
                     TFDYFMQQVMISNCTLFFPIIIALIAGYIITREYTDDTMKNILTIPIPYKQLLSGKLL
                     ILLLLTISFSLIGCVIALVINIIVGFPGVHFGNLLNMFIRVTGANIGIYISVLPIILI
                     FCCSANNFLGGVALAFVYGYFGTFEGTLLNYYPIKASMILVDPTCGAEYGYTYHIFPA
                     FITIVLTFLISMIILANKKKKPGTLTVGKKKKAVRKKGW"
     gene            complement(18872..19681)
                     /locus_tag="PB780_000587"
     CDS             complement(18872..19681)
                     /locus_tag="PB780_000587"
                     /inference="COORDINATES: protein motif:HMM:NF024141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDE0732662.1"
                     /translation="MLKLIKCEFWKLKRKRLLYALMSLSLFFPLILAYMAKAGTGADT
                     TEHYLQTRFDYVYTMMLGYGLVFLLPCLIGIIAAILFFIERDCDTSKNLRTIPVTNTQ
                     LIMAKISMLFIFSVAFCLISTLSVALFCKLFHVGMVYGMTYKIFMSLIFGVLIVAASL
                     PIVFLIIYFNKSFLLSILLAFFYSIFNWGILGTIGTSISAAKIAFLNSFPVICVMNWT
                     SGLMMDNLQKDNLLPEAYAIVPTTCHTIFIMAITVILSLWLIIRFYKKWTR"
     gene            complement(19674..20600)
                     /locus_tag="PB780_000588"
     CDS             complement(19674..20600)
                     /locus_tag="PB780_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDE0732663.1"
                     /translation="MDKNYIIETKNLTKQYGSQKSVADLNIHVQKGRIYGLLGRNGAG
                     KTTTMKMLLGLTKPTSGEVKIWGKSLQGNEKKLLPRIGSLIESPGFYPNLTATENLQI
                     FATLRGIPNRHSIKNTLDLVGLPYKDKKLFSQYSLGMKQRLAIALAVMHDPELLILDE
                     PINGLDPIGIAEVRSFIRELCDTKGKTILISSHILSEISLLADDIGIIDHGALLEEES
                     LAELEQKSNKHIRFTLSDTAQAARILERTFHESHFSIQDDHNLRLHNLDLSVGKIVTA
                     FVENGLEVSEAHTCEESLEDYFKRVTGGEGIA"
     gene            complement(20737..21654)
                     /locus_tag="PB780_000589"
     CDS             complement(20737..21654)
                     /locus_tag="PB780_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDE0732664.1"
                     /translation="MEAVIFLSIIIALFSILVSCFAIRRVKKQIAEITDTLIDVKNGN
                     GNRRILSATNELVAPLAYEINEIVVSYESRLSTVRQTEETNRQLMTSLSHDVRTPLTT
                     LIGYLDAAHKGIVTGKDRDDYIETARRKAHDLKEYIDVLFDWFKLNSNEFAMDINIVE
                     AAELTRNILIDWIPIFEDKQIDYNIDIPEQPFRVKLDTDGYMRILNNLIQNVISHSHA
                     DKIEIILSKQEKNMQIRLADNGIGIEKEDLKHIFERLYKCDKGRSEKGSGLGLSIAHQ
                     LVEKMNGTITANSIQGKGTEFTLLFPLAK"
     gene            complement(21661..22353)
                     /locus_tag="PB780_000590"
     CDS             complement(21661..22353)
                     /locus_tag="PB780_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDE0732665.1"
                     /translation="MNKVLIIDDDKELCVLIKRSVLSEDIEADFCNTGKEGLQKLKEK
                     EYQLVILDVMMPGMDGFETLEEIRKENSLPILMFTSKNDSASKVRGLRAGADDYLTKP
                     FDMDELIARIISLIRRYTRFNQTDGMTQQLEFDGLKIDIENRSVTTENGTFELPPKEF
                     DLLLYCAKNQGKILTKQQIYEEVWREEYFYDDSNIMAIISRLRKKLEVNPGSPKYIQT
                     IKGIGYRFNKEV"
     gene            22695..23072
                     /locus_tag="PB780_000591"
     CDS             22695..23072
                     /locus_tag="PB780_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDE0732666.1"
                     /translation="MAKRPTAKYDFKAFGQAIKAARTERKESRKKVSDEMYISPRYLA
                     NIENKGQQPSLQIFYELVTRYDISVDQFFFPDKSAEKDTQRRQLDTLLDSMSEKGIRV
                     VTATAKEIAEVETEDRGMSRTEF"
     gene            complement(23130..24038)
                     /locus_tag="PB780_000592"
     CDS             complement(23130..24038)
                     /locus_tag="PB780_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015552519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDE0732667.1"
                     /translation="MIQIRKEENQKKQKEKKLKVYKVNTHKKTVIALWVLLAVSFLFA
                     VYKNFTAIDIHTVHETKVIEEQILDTHKIENFVENFAEVYYSWEQSAASIDSRTNALK
                     GYLTGELQALNVDTVRKDIPVSSALTDFQIWEITEEKEQHYQVTYTVEQRITEGESGK
                     TVRSAYQVTVYVDGSGNLTIIQNPTITSVPVKSGYTPKAVQSDGTVDSITTEEINEFL
                     TTFFKLYPTATAKELTYYVNEGVLKTVGKEYIFSELVNPVYNRNGNQVTASLAVKYLD
                     NQTMTTQVSQFNLVLEKNGENWKIVK"
     gene            complement(24055..25059)
                     /locus_tag="PB780_000593"
     CDS             complement(24055..25059)
                     /locus_tag="PB780_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDE0732668.1"
                     /translation="MKLRHLFFACSGVFVMMFSLLLLVVIVFSDEEDGGSGGNLIYGG
                     VSVSQEVLAHKPMLEKYAREYGIEEYLNVLLAIIQVESGGTLEDVMQSSESLGLPPNS
                     LSTEESIKQGCKYFSELLAAAETKGCDLNSVIQSYNYGGGFLDYVAGRGKKYTFELAE
                     SFARDKSSGKKVTYTNPVAVEKNGGWRYSYGNMFYVLLVSQYLTVAQFDDETVQAIME
                     EALKYEGWTYVYGGDSPSTSFDCSGLVQWCYGKAGIALPRTAQEQYNVTQHIPLSEAK
                     AGDLVFFHSTYNAGTYITHVGLYVGNNRMYHAGNPIGYADLTGSYWQQHLAGAGRIKQ
                     "
     gene            complement(25527..27329)
                     /locus_tag="PB780_000594"
                     /pseudo
     CDS             complement(25527..27329)
                     /locus_tag="PB780_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
     gene            complement(27416..29866)
                     /locus_tag="PB780_000595"
     CDS             complement(27416..29866)
                     /locus_tag="PB780_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDE0732669.1"
                     /translation="MAYPIKYIENNLVFNHDGECFAYYELLPYNYSFLSPEQKYQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRAAQERSKQEVTGKLKDIACAKIDAQTEALISM
                     IGENQVDYRFFIGFKLLVNEQEVTMKQFRREAKTAVSDFLHEVNHKLMGDFVSMSNEE
                     IWRFQKMEKLLENKISRRFKVRRLDKDDFGYLIEHLYGQTGTAYEDYEYYLPKKRFQE
                     ETLVKYYDLIKPTRCLIEENQRYLKIEQEDGTVYAAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVTNRKALSTVRNKKKELKDLDNHAWQNDSETSTNVVDALDSV
                     NELESTLDQSKESMYKLSYVVRVTAPDLEELKRRCNEVKDFYDDLNVKLVRPFGDMLG
                     LHGEFLPASKRYLNDYIQYVTSDFLAGLGFGATQMLGETEGIYIGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALATAFVGSLGGGKSFSNNMIVYYCVLFGAQALIVDPKAERGRWK
                     ETLPEIAHEINIVNLTSEEQNRGLLDPYVIMENPKDSESLAIDILTFLTGISSRDGEK
                     FPVLRKAIRAVTNSEERGLFKVIEELRAEGTTISTSIADHIESFTDYDFAHLLFSDGD
                     VTQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     VFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDINEIKKTLAFFGVDSEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPIF
                     EDLFHAFDTRPPVRKEVE"
     gene            complement(29850..30242)
                     /locus_tag="PB780_000596"
     CDS             complement(29850..30242)
                     /locus_tag="PB780_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDE0732670.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFKLPFPITFTQMAWFVVSVFAV
                     MLLGNLPPLSFIDGAFLKYFGVPFALTWFMCQKTFDGKKPYGFLKSVLAYLVRPKLTY
                     AGKPVKLKKEYPAQPITAVRSDIYGISD"
     gene            complement(30336..30824)
                     /locus_tag="PB780_000597"
     CDS             complement(30336..30824)
                     /locus_tag="PB780_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001857838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDE0732671.1"
                     /translation="MRIYIANLGKYNEGELVGAWFTPPVDFEEVKERIGLNDEYEEYA
                     IHDYELPFEIDEYTPIEEVNRLCEMVEDLPDYIQEELSELQSYFGSIEELCEHEDDII
                     CHSGCDDMADVARYYLEESGQLGELPAHLQNYIDYAAYGRDMELEGTFVVTNHGVYEI
                     LR"
     gene            complement(30955..31176)
                     /locus_tag="PB780_000598"
     CDS             complement(30955..31176)
                     /locus_tag="PB780_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732672.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLV
                     VSLVAVGLVFNADGVKDILLELFNKIIGA"
     gene            complement(31228..32439)
                     /locus_tag="PB780_000599"
     CDS             complement(31228..32439)
                     /locus_tag="PB780_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDE0732673.1"
                     /translation="MRFFRQEGFSLNEETWVRDIKEKREVYGISQQKLALAAGITRPY
                     LSDIETGKAHPSEALQEAITEALERFNPDNPLEMLFDYVRIRFPTTDVKHIVEDVLRL
                     KLPYFIHEDYGFYSYTEHYYLGDIFVLVSPELEKGVLLELKGRGCRQFESYLLAQERS
                     WYEFFMDVLMEDGVMKRLDLAINDKTGILNIPHLTEKCRNEECISVFRSFKSYRSGEL
                     VRRGEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKHDIPIADAEVKNRFEIRLKNER
                     AFYAIRDLLEHDNPERTAFQIINRYVRFVDRDDTKPRSDWRISEEWAWFIGEHRGSLK
                     LTTKPEPYSFERTLHWLSHQVAPTLKLALRLDKMNHTQIVHDIITHARLTEKHEKILK
                     QQAAAAKEMVL"
     gene            complement(32603..34000)
                     /locus_tag="PB780_000600"
     CDS             complement(32603..34000)
                     /locus_tag="PB780_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDE0732674.1"
                     /translation="MKQLFHRGKRIRPTDKDLVFHTALASLFPVFLLVVLLFHIRQIA
                     GTNWQEESLSQIVQDVNIPYLLFSMGVAGLVCLTAVLLFWRYRRDEVKQLIHRQKLAR
                     MVLENKWYESEQKKEDAFFKDLSSSRSKETITYFPKIYYRMKQGLLHIRVEITLGKYQ
                     EQLLNLEKKLESGLYCELTDKELKDSYVEYTLLYDTIANRISIEDVQAKDGRLRLMKN
                     VWWEYDKLPHMLIAGGTGGGKTYFILTLIEALLRTNAVLSVLDPKNADLADLQAVMPD
                     VYYKKEDMLTCIDRFYEEMMKRSEDMKLMENYRTGENYAYLGLPAHFLIFDEYVAFME
                     MLGTKENAAVLNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMS
                     EMGYGMMFGETTKDFFLKQIKGRGYVDVGTSVISEFYTPLVPKGHDFLKEIKKLIDSR
                     QGVQAACEAEAAETD"
     gene            complement(34039..34416)
                     /locus_tag="PB780_000601"
     CDS             complement(34039..34416)
                     /locus_tag="PB780_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDE0732675.1"
                     /translation="MRLANGIVIDKEATFGALKFSALRREVHLQNEDGSVSEEIKERT
                     YDLKSRGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFQGAEVDWYIKAE
                     DIVLKKGAAMNPQPPKKDAPPVK"
     gene            complement(34438..34752)
                     /locus_tag="PB780_000602"
     CDS             complement(34438..34752)
                     /locus_tag="PB780_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDE0732676.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKTEQRRINGRMAILSRSFNLY
                     SDVQRADDIVVILPAEAGEKHFDFEERVKLVNPRITAEGYKIGTRGFTNYILHADDMV
                     KA"
     gene            complement(34865..35932)
                     /locus_tag="PB780_000603"
     CDS             complement(34865..35932)
                     /locus_tag="PB780_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009255061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDE0732677.1"
                     /translation="MCALKLGSLFDGIGVFPLAASRNGIIPVWASEIEKSPIAITKRH
                     FPGMEHLGDITKIHGGHIPPVHIITFGSPCQNLSKIGKREGLAGSKSGLFFQAIRIIE
                     EMRCATNGLYPVIAVWENVMGAFSSNDRMDFRAVLQSFSDTEISMPPTGGWANAGMVR
                     GGKTDLCWRLMDAQYWGKPRLVQRRKRIFLVADFRGQRSAEILFKPRDMFPIPPPCTD
                     YGLPAAGASRISLDKTRGKIPIIRPFQERRMRSAAKSGNQSAFLASFGRPDEPFPTLL
                     AKAVNMISFWYEGEEKDGVLRNLTPVECERLMGLPEGWTAYGNLGQPISDNARCKALG
                     NAIALPCADYIMAGIAETIHE"
     gene            complement(36297..36518)
                     /locus_tag="PB780_000604"
     CDS             complement(36297..36518)
                     /locus_tag="PB780_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDE0732678.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            36966..39329
                     /locus_tag="PB780_000605"
     CDS             36966..39329
                     /locus_tag="PB780_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HDE0732679.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKLGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRNDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFHSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAATLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT        11676 a         8284 c         8544 g        11124 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgtgtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccagctagga cgaaatattc
     5701 cagccgatga accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac
     5761 ttgaggggta cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga
     5821 ctttgaaaat cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa
     5881 aagatgaaca agtatttgat gcgatcagtg tcggtagctg gttaaaggta cgaggaagta
     5941 ttcaagaaga tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca
     6001 aacatacacc tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc
     6061 atagcaatat gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag
     6121 gaaaatgggg acaccgagct atcgccatta cagaccatgg tggtgcgcaa gcctttccgg
     6181 aagcacatag tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg
     6241 tcgtagatga tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca
     6301 catacgtcgt atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg
     6361 agctagccgc agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg
     6421 atccaggaca tccattgtca cgaacaacgg tagatctgac tggtatcacg gatgaaatgg
     6481 ttcgagggtc aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata
     6541 caattttggt agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa
     6601 ggtatggtat tcctgaagcg gccaatccag tcattgatac gttggaatta gctagatatc
     6661 tgtatcctca attcaaacgt ttcggattag gtgtgttatc taaaaagttt ggtgttagct
     6721 tggaacagca tcaccgagca atctatgatg ctgaagcaac tggtcactta gcatggatct
     6781 ttgtgaaaga ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata
     6841 tcggagaagg agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa
     6901 atcaggcagg actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg
     6961 aacgcgtccc acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg
     7021 gctcagcttg tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg
     7081 caagaaaccg agcaaaattc tatgattata ttgaagttat gccaaaagcg gtctatgcgc
     7141 ctttgatcga gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt
     7201 tggtagaaat cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc
     7261 tgaacgaaga agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc
     7321 cgttgaatcg tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg
     7381 catttcactt tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa
     7441 tcgcagatat ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc
     7501 caggttcaga agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag
     7561 atccgttacc tgagatcatt caaaaacgat tagaaaaaga gttgaattct attaatggta
     7621 atggcttctc ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg
     7681 gctatctagt aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa
     7741 tcactgaagt taatccatta gcccctcatt attattgtcc agagtgccaa tattccgagt
     7801 tttttgaaga tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat
     7861 gtggtgcacg cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc
     7921 atggagacaa agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc
     7981 ataactatac gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta
     8041 cagtcgccga caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc
     8101 agtttcgaag tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa
     8161 ccggccagca tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta
     8221 cgccgatcca atatccagca gacgatcaaa attcagaatg gaaaacgact cattttgatt
     8281 tccactcgat tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg
     8341 ttattcggat gcttcaagat ttatcaggga tcgacccaca aacgatccct acggatgacc
     8401 cggaagtgat gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt
     8461 attccaaaac agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgt
     8521 tagaagaaac gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg
     8581 gtacagacgt atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg
     8641 cggaagtaat cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata
     8701 gcgggatggc atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg
     8761 atgaattgcg agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata
     8821 tcgattcttg ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat
     8881 tgatggctct tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat
     8941 acttttctgt acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg
     9001 tgaaacaagc aatgaaagaa atcacggaca agggattaga tgcttccgta aaagaaaaga
     9061 atcaactgac agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa
     9121 tgattgattt gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg
     9181 caccatttag agctgtccca agtttgggaa ccaacgtggc gaaacaaatc gttgaagcaa
     9241 gaaaagacgg tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa
     9301 cattgatcga atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat
     9361 tatcgttatt cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga
     9421 gtatggtata gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac
     9481 tctttttaat ggaattacgc acttaatttt taaggaggcg aaacttttga gtagcgtcgt
     9541 tgaaacagtc acagaaatgg tgacaccgat tttagatgaa caaaattttg aacttgtcga
     9601 agtcgaattt gtaaaagaag gaaaaagctg gttcttacga gtatttattg acaaagaagg
     9661 cggtatcgat atcgaagaat gcgcctttgt cagtgagaaa ctgagtgaaa aacttgatgc
     9721 agcagatcca gatccgattc ctcaagctta tttcctggag gtatcctccc cgggagcaga
     9781 gcggccattg aaaaaagaaa gcgactatga aaaggcactt ggtgattatg tccatgtctc
     9841 cttataccaa ccagtagatg gcgaaaaaca atatgaagga tttcttcaat catttgatgc
     9901 tgaacagctt acattgaaaa tacgcataaa aacacgagaa aaagaattga cttttgaccg
     9961 aaaaaatatt gcaaaagctc gcttagctat ccaattttaa tcacggagag gaatcagaaa
    10021 aaaatgagta aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa agggatctca
    10081 aaagaaatcg tgatcgatgc attagaagca gcattggttt ctgcatacaa acgtcactat
    10141 ggccaagcac aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat
    10201 gcggtaaaag aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat
    10261 gcacttttaa tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca
    10321 aaagattttg gacggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt
    10381 gaagcagaaa gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa
    10441 ggaatcgtcg aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca
    10501 gttctttcta aacaagatca aatgccaaat gaattttatc aaccacatga tcgaatcaaa
    10561 gtttatgttt cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc
    10621 catccagacc tattgcgtcg cttatttgaa caagaagtac cagaagtcta tgatggaatc
    10681 gttgaaattg tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca
    10741 aatgatccaa atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa
    10801 gcaatcgtca atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca
    10861 gctgtattca tcgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag
    10921 acaaattcaa aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt
    10981 aaacgcggac aaaatgcacg tttagctgct aaattgacta accataaaat cgatatcaaa
    11041 tcagaatctg acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa
    11101 ctgcatgatg aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca
    11161 ttcaacaatg aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag
    11221 atgaaacaaa ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa
    11281 aaagagatgg tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga
    11341 aaaatgcctg gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg
    11401 gataaaaaaa ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa
    11461 cttcttgact atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag
    11521 ttaatcggca aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca
    11581 cgggtgaaga actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg
    11641 caaccgatgc aagtgagaat acaagaaaaa agataaaaga taagagttca tattatgaag
    11701 ttccttgctt tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca
    11761 aggtattcgg aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt
    11821 aggaaggtga ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat
    11881 ctagtaaaga cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg
    11941 gtgcgattac tagcagtgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga
    12001 aacaacaagc tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac
    12061 gtaaaaaaga aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc
    12121 gcggtcaagg gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata
    12181 cgggaaatac cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta
    12241 ctaacaataa caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta
    12301 atcgttataa taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac
    12361 caccacgcaa attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag
    12421 cagatattgc gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc
    12481 ttggtgtcat ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga
    12541 cggactatgg catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat
    12601 tcttcgaggc agaagaagtc gatccagata aattagtctc acgtccgcca gtagtaacga
    12661 ttatgggaca cgtagaccac gggaaaacaa ccttattgga tacattgcgc cattcacgtg
    12721 taacaagcgg cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg
    12781 atggtaaacc aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg
    12841 cacgtggagc aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca
    12901 tgccacaaac agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg
    12961 ctgtaaataa aattgataaa ccaggtgcaa atcctcagca tgttatgcaa gaattaagtg
    13021 agtatgaatt aattcctgaa gcatggggtg gagaaacgat ttttgttgag atctcagcga
    13081 aattcggcca aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag
    13141 atttaaaagc agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata
    13201 aaggtaaagg tcctgtgtca accttacttg tccaagaagg aacacttcac gttggtgacc
    13261 caatcgttgt cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc
    13321 gcgataaaga agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc
    13381 aagcaggtga ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag
    13441 aacgcgcaaa acgtgcatta ttggaaaacc gtgctgccac aagccgtgtg actttggaca
    13501 acttatttga aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag
    13561 atgtacaagg ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg
    13621 tccgtgtaaa aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag
    13681 cagcagccag caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag
    13741 tgcaagctga tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg
    13801 aagaaatcga aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg
    13861 gtcaaatgat cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct
    13921 atgtaacaga aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg
    13981 taatctatga aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca
    14041 aaatgggtta tgaatgtggt gcgatgattg aaaaattcaa tgacatcaaa gtagatgacg
    14101 taattgaagg atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc
    14161 aatcattatg gctaactacc gtgatcgccg agtcggtcaa gaaatcttaa aagaagtcaa
    14221 tgatattttg cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt
    14281 gaaagttacg ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc
    14341 atcagataag aaaaaagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga
    14401 gttagggcac aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc
    14461 agttgtctat gggaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa
    14521 aaatcccgca aatcctgtga atgtgagggt ttggtaagga tgtttttaag atttaggttt
    14581 tgcggtatag tatgttgcgt actataccgc tttccctttc cacaaagtga cctttgagtg
    14641 gtcgaatact ccgatatggt tatagataat ttcaatttcc tgtaagcgtt taccgctgct
    14701 cttatcagga gcatggacaa taattttgtc gataaactca tgtacaattt ctggtgttag
    14761 ttctgtaagg ttagtgtact tgcggactac tgaaagaaat tgctttacat ccacagattg
    14821 tttttcttct ttctgtaatt ctttctctaa ggaagtctgt tcttcctgca aagtatgctg
    14881 ttcttcctca tatccttttg aaagtttcat aaagcgctca tctgaaagtt ttccactaat
    14941 attgtcttca taaatccgtt gaaaaatttt gtccaactct tgaatacggg aatggatttc
    15001 tgtaagccgt tttttctttt gcgacagttc tttatttctt tgccgcatat ccgtatccat
    15061 gaccatctga acaaattcat cttcataatc tgatacatag cggataactt ctttcaggtt
    15121 ttccagcaca atttgttcga ctactacatt acgaatatag tgcatagtac atgattttga
    15181 attgtgccgg taattgctgc atacaaaatt atgtttctct gcgctctgtg ccatttgcct
    15241 atgaaatgtc attttgcttc cgcaatctgc acagtagagc aaaccagaga acataggcat
    15301 atcccccatt ttggtagggc ggcgttttcc ggaacggatt ttctgtacaa tctcaaaggt
    15361 ttccttgtca atgattgctt catgggtatt ctcaaaaatc ctccattgtt caggactgtt
    15421 atcaattttt ttgcgatttt tataagactt aacatgggtc ttaaaattca cagtatgtcc
    15481 taaataatcc attctttcta agatgtgaga taccacagtt gcactccaca gatacttgtc
    15541 aggtggaaga acggttcgtg gcttttcccc atgctctaac gcataataag tagggcaaag
    15601 aatttttctt tccgttagaa tattggcaat tcgtgtcggt ccataaccat tgacacataa
    15661 acggaagatt tcccgaacca cttcggcggc ttcttcatct ataagccatt cctttttatt
    15721 gtcaggattc ttcatgtagc cataaggagc gtgagaaccg atatgctcgc ctgccattcc
    15781 tttagaacgt ttgactgcac ggattttttt gctggtatcc tttgcgtacc attcgttgat
    15841 tatgttacga aatggggtaa actcattctc ccccacatgg ctatccacgc catcattgac
    15901 agcaataaaa tggacatcca tgttcgggaa cataatttct gtgtacattc ccacctgcaa
    15961 ataatcacgc ccaagtcttg acatatcttt gactataacg cgtttgattc ttcctgcttc
    16021 gacctctgca atcattcttt gaaaatctgg tctgttgaaa tttgtaccag aaaatccatc
    16081 atctacaaaa aacacataag gctgataccc atgttcgatt gcataacgct tcaggatttt
    16141 tttctggtta ataatactgt ttgaatcacc ctcctgttta tcatcttgtg aaagccgaca
    16201 gtataatgcg gttaattcct ctgtttgatt tccaatcgga taaatggaag tgatagtgtt
    16261 gtttgactgc tgtaacataa atacctccta tccgacagtc aaacagacaa tgcttttgca
    16321 actttattgt accgcatttt ctgttttctg tctgccctgt tttatagcaa aattattgtt
    16381 tcaagtcatt acggataagc ctttttactt tcgttactgc gtcttcctgt gcctgttctt
    16441 ttgagcggga ttttaccaca taacaggtgt ttcctatctg gtattcccgt atcatttcag
    16501 gggcaggatt ctttgtgttt tccataagat aacaacctcc tgttattttc tttgaagcaa
    16561 accgaacagc tttcgctaaa gctcatggga atctcacccc tgaatggttc tcatccagcc
    16621 gtaccctttg ccagcggtgc tacatcatca cacggactaa ggctgtacgg aagtatcatt
    16681 atcacaatag aaagttcatg gcggcagctc tgcggtaagc tacttatgga acgctggcag
    16741 gaatcgctat ccggtatcat ccctcctttg gtgggtcacg gcgtgccagc ccaacggctc
    16801 tctttctatc gaaacgtatt cggctgcttt atgctgcgtt gattagacgc tttctttttc
    16861 aaggaacaat ccggctttgt cagcctgtta aaatgcactg gtgataatgc actttaatag
    16921 acgggcaagc tctgtctggg aagcatgggc ttgtccatgc ttccacagga gagcgttatt
    16981 tttactttgg ttccctgatt tcaaaagata aaatctttgc aatcagacgt gtttccatcc
    17041 ggcggcgtag ggtttcatct acaaccatgt gggtgttccc gtattcgtct ttcatgggac
    17101 gcatggaacg gctggcaata aaaccgctgt aatggcgtac aatctggttg actgcttcaa
    17161 tatcgccgtc cgcagccctt acaatcacag gaaacggaat catagggtgc tttgctgtca
    17221 tgctttatcc tccataaact ttttgataaa ttccagtccg gcgtgtcttc ttctctggat
    17281 ggtagaacgg ttgacttcca gaatttttga aatctccgta tcgtcaaatc cgaggaaata
    17341 atatctcagg ataacgtcac gtttctttcc gtccagattt tccaacgctt ctgctaacag
    17401 gctgttttca atgcggacag taaatccatc catttcaaaa gtatggaaat aggatggata
    17461 ggtgtcttcg gaagaaaaca gattgacggt gtaatcgtcc atatcacaaa acagggtttc
    17521 ccgtttgcac tgcctggaca acgctttgag atagtctttg cgttcatctt ccatagcgac
    17581 tttacaaatg taatcaaact ggttctctat ggttgtctga aattcagatg gcttcatcat
    17641 tgctcacccc ctttccagcc ttgaaaggga gcgagttgat agaaatttct gcttcttccc
    17701 cccttttcgc tcttagtccc gacaggacag ggggtgtagt tgcatttttg aataaaaaaa
    17761 tacacccatt ttaaaataaa aatgagtgca tgagaaataa cagtagaata aatatgactt
    17821 gtcagttcat attcaaagtc ggacttccac agtggaggag ctatactttt tttaacgaat
    17881 gcaacctatg tatgctgtat agcagcaaag aaattcccat agcggcagcc ctcctaaatg
    17941 ccgctaattt atgttatagg tagcagtaga caaaaagaag cggcggcttt aaaaattaaa
    18001 gccaccgctt caaaggcgcg tgaaagtgta tctgctctgc gacagctttt tttcttttgc
    18061 cgtcgttcgg caggtactat caatcaaatt atttaccaac ccttttttct aactgctttt
    18121 ttcttcttac caacagtcaa tgtaccaggt tttttctttt tatttgcaag aataatcata
    18181 gaaatcaaga aagtcaaaac tattgtaatg aaagcgggga aaatgtgata agtataacca
    18241 tattccgcac cacaagtagg gtctactaaa atcatgcttg ctttaatcgg ataataattt
    18301 aacagtgttc cttcaaaagt tccaaaatat ccatacacaa aagctagtgc aactccaccc
    18361 aaaaaattat ttgccgaaca acagaatatc agaataatcg gcaagactga aatataaatt
    18421 cctatattcg ccccagtaac acgaataaac atattgagta gattgccaaa atgaactccc
    18481 ggaaatccga caataatatt gattactaat gctatcacac aacctataag actaaagctg
    18541 attgtcaaca gcaatagaat aagtaatttt ccagaaagta actgtttata tggaatagga
    18601 attgttaata tattcttcat agtatcgtct gtatattccc ttgttatgat ataacctgca
    18661 atcagcgcaa taataatcgg gaaaaataag gtacagttac taatcataac ctgttgcata
    18721 aaatagtcaa acgtccatgt ctgtccaccg ttggctgtgg aataaaacag tgacagcaac
    18781 ggagaaagtg ttgtcataat aattccggct tttattacag aatatcgttt taattttaaa
    18841 aattcagttt ttattatatt tctcatatct tttacctcgt ccatttttta taaaagcgaa
    18901 ttatcagcca caaagaaagt ataactgtaa ttgccataat aaaaattgta tggcaagtgg
    18961 ttggtacaat cgcataggct tccggcaaaa gattatcttt ttgcaaatta tccatcatta
    19021 agccgcttgt ccagttcata acacatataa ccggaaaaga atttaagaaa gcaattttgg
    19081 cagcacttat agatgtccca atcgttccca gtatgcccca gttaaaaatg gaataaaaaa
    19141 atgcaagcag gatagaaagt aaaaaacttt tgttaaagta aataattaaa aatacaatcg
    19201 gtaaagaagc tgctacgata agaactccaa aaataaggct cataaatatc ttataagtca
    19261 taccgtaaac cattcctaca tgaaacaatt tacaaaacaa cgcaacagac aatgtgctta
    19321 tcagacaaaa tgcaacgctg aaaataaaga gcatagaaat ttttgccata atgagttgtg
    19381 tatttgtaac tggaattgtt ctaaggtttt tggaagtatc gcaatctctt tctataaaaa
    19441 acaaaatagc ggcaatgata ccaattagac atggaagcaa aaaaactaaa ccatatccca
    19501 acatcattgt atagacataa tcaaatcggg tttgaagata gtgttccgtt gtatctgcac
    19561 cagttcccgc ctttgccata tatgccaaaa tcagcggaaa aaataacgac aatgacatca
    19621 gtgcatacaa cagtcttttt cgctttaatt tccaaaattc gcacttaatc agtttaagca
    19681 attccttcgc ctcctgtcac acgcttgaag taatcttcaa gactttcttc acaagtatgg
    19741 gcttctgata cctccaatcc attttctaca aaagcagtca cgatttttcc tacggacaaa
    19801 tcaagattat gcaagcgtaa attgtggtcg tcctgtatag agaaatggct ctcatggaaa
    19861 gtacgctcta aaattcttgc tgcctgtgcg gtatcagaaa gagtaaatcg gatatgtttg
    19921 ttgctttttt gttccagttc cgcaagactt tcttcttcca atagtgcgcc atggtcgata
    19981 attccaatat catctgctag caaagaaatc tccgaaagga tatgactgga aattaaaatt
    20041 gtttttccct ttgtatcgca aagctcgcga ataaaagaac gtacttctgc aattccaatc
    20101 gggtcaagcc cattgatagg ttcatctaaa atcagaagtt cagggtcgtg cataactgca
    20161 agtgcaatcg caagtcgttg tttcatacca agagaatact gggaaaacag ttttttgtct
    20221 ttataaggca atcctaccaa atccaatgtg tttttaattg aatgacggtt gggtatgccc
    20281 cgtaaggtag caaaaatttg taagttttcc gtagcagtca aattagggta aaaaccgggc
    20341 gactcaatca gactaccgat acggggcaac aatttctttt cattcccctg caaagacttt
    20401 ccccatattt taacttcgcc ggaagttggc tttgtcaatc caagtagcat tttcatagta
    20461 gtcgtttttc cggctccgtt tcttccaagc agtccgtaaa ttctcccttt ctgcacatga
    20521 atatttaagt cagcgacact cttttgtgag ccgtactgct ttgtaagatt ttttgtttca
    20581 atgatataat ttttgtccat attcaaacct cctgttctgt aaactattct atcataccaa
    20641 ccttgcatta accttgccgc aaccttgcat taaccttgca atttgaaata tttctcccca
    20701 aataattaag gctcccgtca aaaggacggg agaaccttac tttgctaagg gaaaaagcaa
    20761 agtaaattct gttccttttc cctgtatgct atttgctgtt attgtgccgt tcatcttttc
    20821 tacaagttgg tgtgcgatag aaagcccaag accgctgcct ttttcagacc gtcccttatc
    20881 gcatttatag agtcgttcaa aaatatgctt caaatcttct ttttcaatcc ctattccatt
    20941 atctgctaat cggatttgca tatttttttc ctgctttgac aggattattt ctattttatc
    21001 cgcatgactg tgagaaatta cattctgaat aagattgttc aatatccgca tatagccgtc
    21061 cgtatcaagt tttacccgga aaggctgttc gggaatatca atgttgtaat ctatctgctt
    21121 atcttcaaat atgggtatcc agtcaatcaa gatatttcgt gtcagctccg cagcttcaac
    21181 aatatttata tccatagcaa actcattaga atttaatttg aaccagtcaa aaagaacatc
    21241 aatatattct ttcaaatcgt gagctttgcg gcgggctgtt tcgatatagt cgtccctgtc
    21301 ttttcctgta acaattcctt tatgtgcagc gtcaagatac ccaataagcg ttgtaagcgg
    21361 tgtccgaaca tcatgggaaa gacttgtcat aagctgtcgg tttgtttctt ctgtctgccg
    21421 gacagtagaa agcctgctct cataagacac aacaatctca tttatttcat aggcaagagg
    21481 ggctaccagt tcatttgttg cagataaaat acgcctgttt ccatttccgt tttttacatc
    21541 aatcaatgtg tctgttatct cggctatctg ttttttaaca cgtcgaatag caaagcagga
    21601 aacaagaatg gaaaaaagtg caattattat ggatagaaaa atgactgctt ccatagaaaa
    21661 tcaaacctcc ttgttaaaac gataaccgat acctttaatc gtttgaatat attttggact
    21721 tccgggattt acctctaatt tcttgcgaag tcggctgata atcgccataa tgttgctgtc
    21781 atcatagaaa tattcctctc tccacacttc ttcataaatc tgttgctttg tcagaatttt
    21841 cccttgattt ttcgcgcagt ataagagtaa gtcaaattct tttggaggca gttcaaaagt
    21901 tccattttcc gtagtaacag aacgattttc aatgtcaatt ttcaacccgt caaattcaag
    21961 ctgctgtgtc attccgtctg tttgattgaa acgggtataa cgccgtataa gagaaataat
    22021 acgggctatt agttcgtcca tgtcaaaagg ctttgttaaa tagtcatcgg ctcctgcgcg
    22081 taagccgcgc actttggaag cgctgtcatt tttagaggta aacatcaaaa tcggcagact
    22141 gttttctttt ctgatttctt ccaatgtttc aaatccgtcc ataccgggca tcataacgtc
    22201 cagtatcaca agttgatatt ctttttcttt taatttttgt agtccctctt ttcctgtatt
    22261 acaaaaatca gcttctatat cttctgataa aacgcttcgc ttaatcagca cacaaagctc
    22321 tttatcatca tctataatca aaactttatt catggtgtag ctcctttcct tgttttgttc
    22381 tcccgtcaat cggtttttcc gcgtcctttg gtatcagcca catataccca agttttgaaa
    22441 caccggggat gcggttctct ctgcatagta tctgtacacg ccgttcagaa atcccccatt
    22501 tcttcgctgc ttcggggcaa gacatatatt ccataacacc caagtccttt ctacaaatca
    22561 tatcagtata attatagtcg ggcatacgaa taatagcaag tgcaagcata tgaattatta
    22621 tggctataaa taggaactgt atagacgtat tcagtatgaa agaggaacag tagaatgtat
    22681 atgggcgaat aaatatggca aaaagaccaa cagctaaata tgactttaag gctttcggac
    22741 aagctatcaa agcagcgaga accgaacgaa aagagagccg taaaaaagta agtgatgaaa
    22801 tgtatatctc accacgttat cttgctaata ttgagaataa aggacaacag ccgagtttac
    22861 aaatttttta tgagcttgta acacgctatg atatatccgt agaccaattc tttttcccag
    22921 ataaatcagc ggaaaaagat acacaacgta ggcagcttga tacactgctt gatagtatga
    22981 gtgagaaagg aatacgggta gtcaccgcca cagcaaaaga gatagccgaa gtcgaaacag
    23041 aggacagggg aatgtccaga acagaatttt aacgtgaaga cacattgtaa tctttcagga
    23101 aatacaatgt gtcttctgtt ttatatagct tatttcacaa tcttccagtt ttccccgttc
    23161 ttttccagta caaggttaaa ctgtgatacc tgtgttgtca tggtctggtt atccagatat
    23221 tttactgcaa gggaagctgt cacctgattt ccgtttcggt tataaaccgg atttaccagt
    23281 tccgagaaaa tatactcttt tcccacagtt ttcagcacac cctcatttac ataataggtc
    23341 agttcctttg cggttgccgt agggtacagc ttgaaaaagg tggtgagaaa ttcgttgatt
    23401 tcctccgtag tgatggaatc caccgtaccg tcactttgta ccgcttttgg cgtatagccg
    23461 gattttacgg gaacgctggt aatggttgga ttctggataa tcgtcaggtt tccagagccg
    23521 tccacataga ccgtcacctg ataggcggaa cggacggttt taccggattc tccctctgtg
    23581 atacgctgtt ccactgtata ggtcacttga taatgctgct ccttttcttc tgtgatttcc
    23641 catatctgaa aatcggtcag ggcagacgat acggggatgt cctttcggac agtatctaca
    23701 ttcagggctt gcagttctcc ggtgagatac ccttttaaag catttgtccg gctatcaatg
    23761 gaagcggcgg actgttccca tgaatagtag acttctgcga aattctccac aaaattctct
    23821 atcttgtgag tgtcgaggat ttgttcctca attactttgg tttcatggac agtatgaatg
    23881 tctatcgccg tgaaattctt atagacggca aataggaagc tcaccgccag cagcacccac
    23941 agggcaatca cggttttttt gtgggtattg actttgtaaa ctttcagttt cttttctttc
    24001 tgctttttct ggttttcctc ttttctaatc tgaatcatat cggttcatcc tttcttattg
    24061 tttgattcgt ccggctccgg caaggtgctg ttgccagtaa gagcctgtca ggtctgcgta
    24121 gccaatggga tttccggcat ggtacatccg gttatttccc acatacagcc ccacatgggt
    24181 gatgtaggtt ccggcgttgt aggtggagtg gaaaaagacc aaatcccctg cttttgcttc
    24241 ggacaggggg atatgctgcg tcacattgta ctgttcctgt gctgtccgtg gcagggcgat
    24301 tcctgccttg ccatagcacc actgcaccag tccagaacag tcaaaggaag tggatgggga
    24361 atcgcccccg tacacatacg tccagccctc atactttaag gcttcctcca tgatagcctg
    24421 taccgtttca tcatcaaact gtgccacggt caggtattgc gataccaaga ggacatagaa
    24481 catatttcca taggaatacc gccagccccc gtttttctct accgcaacag ggttggtgta
    24541 ggtgactttc tttccgctgg atttgtccct tgcgaagctc tctgccagtt caaaagtgta
    24601 ttttttccca cgccctgcca catagtctag gaaaccgccg ccataattgt aggactgaat
    24661 cacactgttt aagtcacagc cttttgtttc tgccgctgcg agcagttccg agaaatattt
    24721 gcagccctgt ttgatggatt cctctgtgct aagggagttt gggggaagcc ccagagattc
    24781 cgaggactgc ataacgtctt ccagtgtgcc gccggattcc acctgaatga tggcgagcag
    24841 cacatttaag tattcctcta tgccatattc ccttgcatac ttttccagca taggcttatg
    24901 ggcgaggact tcctgtgata cgctcacacc cccatagata aggtttccgc cgcttcctcc
    24961 gtcttcctca tccgagaaga caatcaccac caaaaggagc agggagaaca tcatcacgaa
    25021 cacgccggaa caggcaaaga aaaggtgtct taacttcatg gcttctcccc tttcttttgt
    25081 ttggaagccg ctgttactgt ttttgtccac tcctttctgg cggtggtacg ccggatggta
    25141 aagttggatt cttttaccgc aggggcagcc cgttttcttt ccggtatcac aggagccgta
    25201 tcattctgga caggtcttgt cggatgggga gcagcaggtg tagtagttgt cttttgtctt
    25261 tcatctggtt tgtctgggga agccggaggg actatctggc gttctgtcct tacaggcggt
    25321 gtctgttcat ggtggatgga agccgctttt accaccggaa ccgaagaccg ttctgccaca
    25381 gttcccccat aaggaacctg tccccgttcc ctgatggatg gggaagcagg ctgcatggct
    25441 ggttttcctg catgatgtcg gaaatcttct ggctgctttt cagtaacagg gcgttcatgg
    25501 acaggagccg cccctttggg ggcttcggaa gctctttgtt gcggctgttt tgcctgttcc
    25561 agttccgccc tgcgttctgc aatggtctgt ctgcggcttt ctgcctgtgt gttccgctgt
    25621 tcggctctgg cagttcgggt ctgggtcacg ctggaagtaa aatcccttac accctctgat
    25681 acctgtgttt tcccgtggta aagggcgtac tttgcattga caggcaaatc ctttgcctgc
    25741 tcccgaagct gtccggcagt atcggctatc ttatctttgg tatctgccgc cgcacccaca
    25801 gcagagccag cccgtttcca tacggattct ttttccggtg ctgcctgtcc gtctggtctg
    25861 ctgtggtctg cctgtgtccg tttggaagag ccggagtggg aagcatttct ctggtctgag
    25921 ccagcctgtt ttcctgcgtg gtatgcagcc gttccagctc caagagccgc cacggaacgt
    25981 cctaacttat gctgtaaacg gtgcatatgg gcgtgcatga gcatacgggg tcttcgcatg
    26041 atacggctcc ccatgctttg ggaatcgctg ctctgtaagg agaacatccc catcaaatcc
    26101 cccagcttga aatagattcc ggcaaaagtc actatctgca agaacgccgt caggaagaac
    26161 ggatattccc cagacaggtt gtagagcatg gtggaaatgc tgaacgctac cgtgataatc
    26221 agggtgattc cggctctggt aaggatagtg ttgaacagtt tcgtaatggc acgctttgac
    26281 atcccctcaa aagagggaac catgctaagg agaaagctca caggcaggaa catggcgtaa
    26341 atgataaaaa gcacctgtga gaaaatcatt atccccgtga gcaggaatac aaaaacggag
    26401 ataccgatat tgaacatgaa caggaagaag acggttccca atcggttgat ggtctttgtg
    26461 atggtaaggt tcgtgttttc cctgtcttcg atttcctcca ctacgatttc ttccctgtcc
    26521 tgtccgttgt tttcgtctgg actggttgaa agtaggtttt ccacacggtc agctccaatg
    26581 ctttccacat ccgaattgcc gtattgcagc aggagccacg gctgcttgac ctgaatggaa
    26641 aacaggctgt cccgtatcaa atccacgctg tcttttcctt gactttccga gttaggcagc
    26701 acaatctttg taccaagggt caggctggca ttactgatgt ctgcggaaaa ttcattgatt
    26761 ttcccgatat aatcaggggc ataagcgata aaggcagcgg acagcacgaa caccaccatg
    26821 aaattgacaa cggcatggat ggcttttgtg gtttcccgtt tgataagccc tgtgtaggca
    26881 acgtaaatcc ccaccaccag aatgagaatc agcaggaacc cgatataaaa tccctccgag
    26941 gacaagccgc ctgtggtaac gccagcaagg gtctgcatat ttttcccgat agaatccgcc
    27001 gtagaggaaa tgaagtccag cgagtaggct tcctgaatca gataccctgt ggcgttggaa
    27061 aggtacaagc tgattgtcca gataaaattt gtgatggcat acagcccgta catcacctgt
    27121 tttccgatac cgtccagcca gttccacggg agccagtccc atccgctgtc cacataaaaa
    27181 tcaagctggt agttgtccag aggatatttg ctgtattcgt tggcagcgtc caccgtatca
    27241 tccaccaatc cggcagcgtg ggcggctgtg ccgaagactg ccagcagcag gaggatgagc
    27301 aggagtgcca gaagcaccct gccaatcact tttcctgccg tcttccaaga caagcccttt
    27361 tttgtgtgat gaggaactgg gaattgcggt cggttggaag ttctatgtat caccatcatt
    27421 ccacctcttt tcttacgggc ggtctggtat caaaggcgtg gaacaggtct tcaaagatag
    27481 gatggaactg tatcacgccc acacgcccgt acaaatcact gataaggcac tgtccgtttt
    27541 ccaagtcacg gagccgcttc tggttgtttt catcttcgga atccaccccg aaaaaggcga
    27601 gggtcttttt gatttcgttg atgtctgtgg aacggaacgc aaatttcaag cccagattgt
    27661 ttttcagctt ttcatccagc aaatcgtccg tgttctgggt gacaaagtaa acgcctgcgt
    27721 tcatggcacg tccggcacga accagcttca tagagagggt cttgccctgt gccacctgta
    27781 agaaactcca tgcttcgtcc aaatccacaa tcttaaacac gctgcggtca gtgtggatga
    27841 agtcgagggc aaaggtgctg attacaatga gcattgccac ggaaagcagc tccattgtgg
    27901 tgtattcttc aaacgaggtt tccttatccg gcagtaccaa atccgccacc tgaataatat
    27961 tgagctgctt ttcaaggctg atggactgtg tgacatcccc atccgaaaag agcaggtgtg
    28021 caaagtcata gtccgtaaag gattcgatat ggtcggctat gctggtgctg atggtggttc
    28081 cctccgcccg aagttcctca atcactttga aaagcccccg ttcttcactg tttgtcaccg
    28141 cacggatggc tttccgaaga acagggaact tttccccgtc acggctggaa atgcccgtta
    28201 aaaaagtcag gatgtcgatt gccagtgatt cggaatcctt tgggttctcc ataatcacat
    28261 atgggtcgag taagcccctg ttctgttcct ccgaggtcag gtttacgata ttgatttcat
    28321 gggcgatttc cggcagggtt tctttccagc gtccccgttc cgctttcggg tcaacaatga
    28381 gagcctgtgc cccaaataat acacagtagt agacaatcat gttgttgctg aatgattttc
    28441 caccgccgag ggagccgaca aaagcggtag caagggcgtt ggtgacggag ccttttaccc
    28501 cttgactggc aagggcaggc tttaagtaca cgtttcttcc tgtatcaagg ctgtacccga
    28561 tataaatacc ctccgtttct cctaacatct gggttgcccc gaagccaaga ccagcgagga
    28621 agtcactggt gacatactgg atgtaatcgt tcagataccg tttgctggca ggaagaaatt
    28681 ccccatgcag ccccagcata tctccaaagg ggcgaaccag tttcacgttc aaatcgtcat
    28741 aaaaatcttt cacctcattg cagcggcgtt tcagttcttc caagtcggga gccgtcacac
    28801 gcaccacata ggacagcttg tacatggatt ccttgctctg gtctaaggtg gattccagct
    28861 catttacgct gtctaaagcg tccaccacgt ttgtactggt ttcgctgtca ttctgccatg
    28921 cgtggttgtc caaatctttc agctcttttt tcttgttccg caccgtggag agggctttcc
    28981 ggtttgtgac aatctccaca ttcatagagg tatcaatggg aaaggtaaat tgctgctgtt
    29041 gatagtaaaa gatttccgag gatgggaagt ccagctcccc cacaatgctg ttgatggtaa
    29101 agtaggcggc atacactgtc ccgtcttcct gttcgatttt cagataccgc tggttttcct
    29161 ctatcagaca acgggtgggt ttgatgaggt catagtattt caccagcgtt tcttcctgaa
    29221 accgcttctt cggcaggtaa tactcataat cttcgtaggc ggttccggtc tgcccgtata
    29281 aatgctcaat caggtagcca aaatcgtcct tatccagccg ccggactttg aagcggcgtg
    29341 agattttatt ttccagcagt ttttccatct tctgaaaccg ccagatttcc tcattgctca
    29401 tggaaacaaa atcccccatg agcttgtggt tgacttcatg gaggaagtcg gaaacagcgg
    29461 tctttgcttc cctgcggaac tgtttcatgg tgacttcctg ctcattgaca agcagcttaa
    29521 agccgatgaa aaagcggtag tccacctgat tttcccctat catggaaatc agggcttcgg
    29581 tctgtgcgtc aatctttgca caggcaatgt cctttaactt gcccgtcact tcctgtttgg
    29641 agcgttcctg tgccgcccgt atgctggatt ctgtgctgat ttgcagggcg tgaatcttcc
    29701 cgtccctgtt ctgggcgata agctgccgga aggaatcgtg tacctgatat ttctgttcgg
    29761 ggcttaaaaa ggaatagttg tagggaagca gctcatagta agcgaagcac tccccgtcat
    29821 ggttgaacac aaggttattt tcgatatatt taatcggata tgccataaat atcactcctt
    29881 actgccgtga tgggctgtgc cggatattcc tttttcagct tcacgggctt tcctgcatag
    29941 gtcagtttcg ggcgtaccag atatgccagt acggatttca ggaagccata aggctttttc
    30001 ccgtcaaagg tcttctggca catgaaccaa gtgagggcaa agggtacgcc gaaatatttc
    30061 agaaatgccc cgtcaatgaa cgaaagggga gggaggttcc ccaagagcat aactgcaaac
    30121 accgatacca cgaaccacgc catctgtgtg aacgtgatgg ggaatggcag cttaaaatca
    30181 ttgatggaat agagtacctt ttccaccgac cagatactgg tatagcttcg tattttcttc
    30241 atgttgtccg tcctttctaa aagatggggg cagcaaaaga gccgcccctg aataaaaaaa
    30301 tccgcctgta tttcctgaaa gagatacaga cggggctacc gtaagatttc atatacgccg
    30361 tggttcgtga caacaaaggt tccctccaat tccatgtcac gcccataggc tgcatagtcg
    30421 atatagtttt gcaagtgtgc cggaagttcg ccaagctgtc cgctttcttc cagatagtag
    30481 cgagccacat ccgccatatc gtcacagccg gaatggcaaa taatatcgtc ttcatgctca
    30541 cagagttctt cgatactgcc aaagtaggat tgcagctctg atagttcctc ctgaatgtaa
    30601 tccggtaagt cctccaccat ttcacacagg cggttgactt cctcaatggg ggtatattcg
    30661 tcaatctcaa agggcagctc ataatcatgg atggcgtatt cctcatattc atcattcaag
    30721 ccgatacgtt ccttgacttc ctcaaagtcc accggcggcg tgaaccatgc ccccaccagt
    30781 tcgccctcat tgtattttcc taagttggca atgtaaatcc gcatttcttc cacagtgtca
    30841 cctccattct ctgtcatgct ccttttgggg agttacgctc cccaagcccc tagtgtactt
    30901 ccgccatgaa gcaatcagag cttgctccat aacggaagtg tgatatagtt tttcctatgc
    30961 accgataatc ttattgaaca gttccagtaa gatgtccttt acaccgtccg cattgaagac
    31021 cagaccaacc gctacaaggg acaccactaa aaagccgata agtttggaaa attccctctt
    31081 aaatccgagg tacagtccaa tcaccacgat tgccagcagc acaagggact gggcgttgga
    31141 taaaaaccag ttgtaaagat tctgtccgaa attcataaaa gctcctttct ttgctgtaag
    31201 atttcttagc ttccaagatt gccgccgtta aagcaccatc tcctttgcag cggcggcttg
    31261 ctgcttcagg attttttcat gcttctccgt cagccttgca tgggtgatga tgtcatggac
    31321 aatctgggtg tggttcatct tatccagacg gagagccagc tttaacgtgg gggctacctg
    31381 atgggagagc cagtgcaggg tgcgttcaaa ggaatagggt tccggtttgg ttgtcagttt
    31441 caggcttccc ctgtgttccc cgataaacca cgcccattcc tcactgatac gccagtcaga
    31501 acggggcttc gtatcgtccc tgtccacgaa ccggacatag cggttgatga tttgaaaggc
    31561 tgtccgttct ggattgtcat gttctaacag gtcacggatg gcgtaaaagg cacgctcatt
    31621 tttcagccgg atttcaaagc ggttctttac ttctgcgtcc gcaatgggaa tatcatgctt
    31681 tttgtactgc tcatagtctt tttcataaat gcagaagtac acctcacttt ggagggagcc
    31741 gatatacagg gtgttcccca tacattcttt ttccccacgc cgtaccagtt cgccgctgcg
    31801 gtagcttttg aagctgcgga agaccgagat acattcctca ttccggcact tctctgtcag
    31861 atggggaatg ttcaggattc ccgttttgtc attgatggca aggtcaagcc gcttcatcac
    31921 gccgtcctcc atgagaacgt ccataaaaaa ctcataccag ctccgttcct gtgccagaag
    31981 atagctttca aactggcggc agccacgccc tttcagttcc agcagcaccc ctttttccag
    32041 ttccggcgat accagaacga aaatatcacc cagatagtaa tgctccgtgt aagagtaaaa
    32101 tccgtagtct tcatggataa agtaaggcag ctttagccgc agcacgtctt ccacgatatg
    32161 cttcacatcc gtagtgggga agcgaatcct cacatagtca aagagcattt caaggggatt
    32221 gtctggattg aagcgttcca gagcttccgt gatggcttcc tgtaatgctt cggatgggtg
    32281 agccttgccc gtttcaatat cgctcaggta cggacgggtg attccggcag ctaacgcaag
    32341 tttctgttgt gagataccgt aaacttcccg tttttctttg atgtcccgta cccatgtttc
    32401 ttcattcagt gaaaatccct cctgtctaaa aaagcgtatg tcaactttca gaaccttgtt
    32461 gacacacgcc ggatatggga aaatcccttg tgtaccgtgg gattctgccc tgttgtttgg
    32521 ttgttgcctg tcgatttgta cccctctgtt agatacgagg ggttcctgct ggcgtgcctt
    32581 gcggcacacc agccacagca ggtcagtccg tttctgcggc ttccgcttcg cacgccgcct
    32641 gcactccctg cctgctgtct atgagttttt ttatttcttt gaggaaatcg tgtccttttg
    32701 gcacaagggg cgtgtaaaac tccgagataa cgctggttcc cacatccaca tagccacgcc
    32761 cctttatctg cttgaggaag aagtcctttg tggtttcccc gaacatcatc ccatagccca
    32821 tttccgacat ccgccccaga gccacacgga aattgaactg gtcacggatt ccgtccccca
    32881 gatactttgc gtccggacgc tggcaggcga gaatcaggaa gaagccagcc tgccgcccaa
    32941 gcataacgat ttgctttaat ttgttgagga cagcagcatt ttcctttgtg ccgagcattt
    33001 ccataaatgc cacatattca tcaaagataa ggaaatgtgc cggaagcccc agataagcat
    33061 agttttcccc tgtccggtaa ttctccatga gcttcatgtc ttcgctgcgt ttcatcattt
    33121 cctcatagaa acggtcaata caggtgagca tatcttcctt tttgtagtac acatccggca
    33181 taaccgcctg taaatccgca aggtcggcgt tcttcgggtc taacacggac aggacggcgt
    33241 tggtgcggag cagggcttca ataagggtca ggatgaaata agtctttccg ccgccggttc
    33301 ctccggcaat gagcatatga gggagcttgt catactccca ccagacattt ttcataagcc
    33361 ggagcctgcc gtcctttgcc tgcacgtcct caatggagat acggttggca atagtatcat
    33421 aaagcagggt gtactccaca taggaatcct ttaattcctt atccgtcagc tcacagtata
    33481 agccgctttc cagctttttc tccaagttta agagctgttc ctgatatttc cctaaggtga
    33541 tttccacacg gatatggagc aagccctgtt tcatccggta gtagatttta ggaaagtaag
    33601 tgatggtttc tttggaacgg ctggaagaca agtccttgaa aaaagcgtct tccttttttt
    33661 gctctgattc ataccacttg ttttccaaca ccatccttgc cagcttttgg cggtggatga
    33721 gctgctttac ttcatccctg cggtatcgcc agaacagaag aacggctgta agacacacca
    33781 accctgccac gcccatactg aataacaggt aagggatgtt tacatcctgt actatctggg
    33841 agagggactc ttcctgccag ttggttccgg caatctgccg gatatgaaac agcaggacaa
    33901 ccagcaggaa gacagggaac agagaagcaa gggcagtatg gaatacaagg tctttatccg
    33961 tgggacggat acgttttcca cggtggaaaa gctgcttcat gggaacaatc ctcctttcgc
    34021 tttcttctta tattcctgtt attttacagg gggagcgtct ttcttcggcg gctgtggatt
    34081 catggcagcc ccttttttca ggacaatatc ctccgccttg atgtaccagt ccacctctgc
    34141 cccctgaaag gtagccgttg ccacggtatc tgccacagga ttgataagtt ctacctctgc
    34201 gttgtaatcg aactccttta acggcacgct ggcagggatg gaaacctgaa tcatacgtcc
    34261 ctgtcctctg gattttaagt cataggtacg ctccttgatt tcttctgata ccgagccgtc
    34321 ttcattctgg aggtgaacct cacggcggag ggcagaaaat ttcaatgccc cgaatgttgc
    34381 ttctttgtca attacgattc cgtttgctaa tctcatagtt tgatgtcctc cttttcctta
    34441 cgcttttacc atatcgtcag cgtgcaaaat gtagttggta aagccccttg tgccgatttt
    34501 atagccctct gcggtgatac ggggattgac gagcttcaca cgttcctcaa agtcaaaatg
    34561 tttttctccg gcttcggcag ggaggataac tacaatatca tccgctctct gtacgtccga
    34621 atacagattg aagctgcggg agagtatcgc catgcgtccg ttgattcttc tctgttctgt
    34681 tttgtcttct ccggcaaact ccaagttgcc gaatgtcttt tccatgttgg ggataacgaa
    34741 ttttaattcc atatagatgt acctgtcctt tctgaaataa tatagttggt tcgatagctt
    34801 ctgttgatgt tccacccgac aacggaggag aacgattttt ctctatcata caaaaccgcc
    34861 tttcttattc atgtatggtt tctgctattc ctgccatgat gtaatcagca cacggcaggg
    34921 cgattgcgtt tccaagtgct ttgcaccgtg cattatcact gatgggttgt cctaaatttc
    34981 catatgccgt ccacccctcc ggtaatccca tcaggcgctc acactcaacc ggtgtcaaat
    35041 ttcggagaac tccgtctttt tcttccccct cataccagaa tgaaatcata ttgactgctt
    35101 ttgccagcaa agttggaaaa ggttcatctg gccgtccgaa acttgcgagg aaagcagact
    35161 ggtttcctga ttttgcagca ctccgcatac gccgttcctg aaaggggcgg atgatgggta
    35221 tcttccccct tgttttatca agagatattc gactggctcc ggcggcgggc agcccgtagt
    35281 ctgtgcaagg cggaggaatc gggaacatat cgcggggctt aaatagtatt tctgcggaac
    35341 gctgtcctct aaaatccgcc acaaggaaga ttctttttct tcgctgaaca agccggggct
    35401 ttccccaata ctgggcgtcc atgagccgcc agcacaggtc agtttttccc cctcgcacca
    35461 taccggcgtt agcccatcct ccagtaggag gcattgaaat ttcggtatcg gaaaatgatt
    35521 gcagcacggc tctaaaatcc atccggtcat ttgaagaaaa cgctcccatg acgttttccc
    35581 aaacagcgat aactggatat aacccatttg tagcacacct catttcttct ataattctga
    35641 tagcctgaaa aaacagacct gatttgcttc cagccagtcc ctcacgtttc ccgattttag
    35701 aaaggttctg gcagggagag ccgaatgtaa taatatggac aggagggatg tgtcctccat
    35761 gtatctttgt aatatcccct aaatgttcca tgccggggaa atggcgtttt gttatggcaa
    35821 tcggggattt ttcaatctca cttgcccaaa cgggaatgat tccattacgg gaagccgcaa
    35881 gagggaacac gccgattccg tcaaacaggc ttcctaattt aagggcacac atagaccgcc
    35941 tttcgtttta ctttttcatg tgttatttcc atttttctgc tcctttccag acaataaaaa
    36001 aaacaccatc aatggaagtc cattcaatgg cgtttgttgt tgtctgctaa tgtttcaagg
    36061 taaagcagaa agctcaatag aaaaagacgc aaatctcaaa aatgcgtctt tttctatctt
    36121 catattcatt ttttataaaa gcactgtctg cctgctgtct gcaaagcctt gattttactg
    36181 gttttctact accatcctta tcaaagcctt gctaaatcag gggttgcggg atttttttgt
    36241 tttcattaag acgtattacc taagagttcc atgactttag tctggtagcg tgtcagctaa
    36301 ccaaaaaaga gaaaaagaaa agtaaaaaat tcgactttct tttttctata gtatataaat
    36361 ttttgcatat agataaagat caagataccg ataacggaag ctagattgta atctatttta
    36421 gtgacagtca ggtatagcca agtaactaga agatcagtgg aacctgcacc ataaaagttt
    36481 gagtttgtcg gtccgttgac agttaaaaga aaaatcacgt tgaagttatt gatatttccg
    36541 attacgccgc ttcgatttgg tcacgatcca aattttggat aatccctgta gaaattagca
    36601 tagtagctgg gataccaatt cacatattga caaagataac agagactttt acataaaacg
    36661 gatcaatcag gaaaggaatt gctcgatcca gccccagttt tccaacaatg tgttaactgg
    36721 cctagcgtca ttcagcaaat tatgcatgat gaggagcgaa atgaactgcg gaacagccat
    36781 tgtgataacg acaagttttt tggatcaatg taaaaaacgt cagcgaaagg aaaaatttcg
    36841 tggtttttta tacctgttca tttggctaga tataaaatcc aaacaaatga acagaagtgc
    36901 ttattaggtt tcaattggga gtatctgcgc tagaataaga gtaataatga aaaggagtgt
    36961 tatgtatgga ttattctagt aaagaatact ttgataaaat gacggcttgg tggcgtgctg
    37021 ctaattatct ttctgttggg caactttatc ttaaagataa ccccttactg agacgtacac
    37081 tgaaaccaga agatgtcaaa aaacatccaa tcggtcattg gggaacgatt cctggccaaa
    37141 attttattta tgtccatttg aaccgagtca tcaataaata tgatttgaac atgttttata
    37201 tcgaagggcc aggacacggc ggacaagtca tggtatcgaa tgcttattta gatggaagtt
    37261 acacagagat ctatccagaa gtaacggaag atgagacagg aatgcaaaaa ttattcaagc
    37321 gattttcatt tcctggaggc attgcttctc atgcagcacc agaaacacca ggttctatcc
    37381 atgaaggcgg agagcttgga tactctcttt cacatgcagt cggagctgta ttagacaatc
    37441 ctgaagtaat ctctgctgtc gtgattggtg atggtgaagc agaaaccggt cctttagctg
    37501 gaagctggtt ctcaaatgtc tttatcaatc cagtgataga tggagctgtt ttaccgatcc
    37561 ttcatttaaa tggtgcaaaa atagccaacc caacgatctt ggcacgtaaa tcagatggag
    37621 aattagcaaa ttactttaac ggacttggct gggagccttt ctttattgaa ggaaatgatc
    37681 cagaaaaatt aaatcctgtt atggctgaaa aaatggatca agcaatcgag aaaatcaaat
    37741 ccatccaaaa agaagctcgc ctgaagacag caacagatgt cgtgatgcca aaatggccag
    37801 tattgatcgt acgcacacca aaaggatgga ccggtccaaa agaatgggac ggggaaccaa
    37861 ttgaaggaac cttccgagca caccaagtac cgattcctgt agatcaagaa catatggatc
    37921 atgcagatgc tttactccgt tggctgaaat cctatgaacc agaaaaatta tttgatgcac
    37981 aaggacgtat tttagaagaa atcagagaaa ttgcgcctac tggtgaccaa agaatggcaa
    38041 aaaatcctat caccaatggt ggaatcgatc caagaccgct tattatgcca gattggaaaa
    38101 aatatacttt gcaatttgaa aaactaggct ccgttaaagc agaagatatg accgaacttg
    38161 gtaaattcgt tcgcgagatc attgagaaaa atccagaaaa tttccgtata tttggaccag
    38221 atgaaacaaa atcaaaccga ttgaatcaag ttttcaaaac gacaaatcgc caatggatgg
    38281 aaaaaatcga accagaaaat gatgaatggc tttcgccatc tggtcgcgtc atcgacagtc
    38341 aattatcgga acaccaagat gaagggttct tagaagggta cgttctgact ggacggcatg
    38401 gtttttttgc aagttacgaa tcattcttgc gagtggttga ctctatgttg actcagcact
    38461 tcaaatggat acggaaatca catgaccttt cttggcgaaa tgattatcct tcgctaaatc
    38521 tcattgcaag ctcaacagtc ttccagcaag atcacaatgg atatagccac caagatccag
    38581 gaatattgac acatttggca gaaaagaagg cggagttcat ccgtgaatat ctgcctgcgg
    38641 atgcaaatac attgctcgca gtcatggata aagcattcca ctcgtcagaa aaaatcaatt
    38701 tgatcatctc ttccaaacat ccgcgtgctc aattttactc agcagaagaa gcagccgtct
    38761 tagtgaatga aggcttgaaa atcattgatt gggcttcgac agcaaaagag gaggaacctg
    38821 aattggtcat tgctgctgca ggaacggaat ctaacttaga agcattagca gctgcaactt
    38881 tgctgctgga agaattccca aaactaaaaa ttcggttcat taatgtcgta gatttattga
    38941 aattacgtca tccaagtcaa gatccgcgtg ggttgagcga tgaagaattt gatcaatatt
    39001 ttacaaaaga caaaccgatc ttatttgctt tccacggtta tgaaacattg gtccgcacga
    39061 ttttctttga tcgtcataac catcatttaa tgattcatgg ctacaaagaa aatggagata
    39121 tcacgacacc atttgacatg cgtgtagtaa atgaacttga tcgttatcat ttggcaaaag
    39181 acgcagcttt aaaaatcaaa ggaagtcaag cagaagattt tgcggaaaaa atggatcaga
    39241 aattacaaga acatcaaaac tatattcgtg aaaatggtat tgatctgcca gaagtattgg
    39301 attggaaatg gaaaaatctt gatcaataaa atgataaaat cgatcccgca aaacagaatt
    39361 ttttacattg attaagaaac tgaaaaaata aataaaaaaa acggaaaaaa gactggaaga
    39421 atggacagtc ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca
    39481 cccataaatc caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa
    39541 aaaacataac atgatttcta tttacgtgag catgaatttt gttacgtttt tattgccgaa
    39601 aaataaaaac cgttcattat gatgtcaa
//
LOCUS       DAMEET010000013        38669 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.013, whole genome shotgun sequence.
ACCESSION   DAMEET010000013 DAMEET010000000
VERSION     DAMEET010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 38669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.013"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(369..995)
                     /locus_tag="PB780_000606"
     CDS             complement(369..995)
                     /locus_tag="PB780_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732680.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(1290..1625)
                     /locus_tag="PB780_000607"
     CDS             complement(1290..1625)
                     /locus_tag="PB780_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDE0732681.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(1612..1896)
                     /locus_tag="PB780_000608"
     CDS             complement(1612..1896)
                     /locus_tag="PB780_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDE0732682.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(1952..3475)
                     /locus_tag="PB780_000609"
     CDS             complement(1952..3475)
                     /locus_tag="PB780_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDE0732683.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(3468..4643)
                     /locus_tag="PB780_000610"
     CDS             complement(3468..4643)
                     /locus_tag="PB780_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDE0732684.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(4647..4832)
                     /locus_tag="PB780_000611"
     CDS             complement(4647..4832)
                     /locus_tag="PB780_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732685.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(4798..6492)
                     /locus_tag="PB780_000612"
     CDS             complement(4798..6492)
                     /locus_tag="PB780_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDE0732686.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(6489..6962)
                     /locus_tag="PB780_000613"
     CDS             complement(6489..6962)
                     /locus_tag="PB780_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HDE0732687.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(7031..7186)
                     /locus_tag="PB780_000614"
     CDS             complement(7031..7186)
                     /locus_tag="PB780_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HDE0732688.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(7320..7700)
                     /locus_tag="PB780_000615"
     CDS             complement(7320..7700)
                     /locus_tag="PB780_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDE0732689.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(7704..7919)
                     /locus_tag="PB780_000616"
     CDS             complement(7704..7919)
                     /locus_tag="PB780_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732690.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(7922..8329)
                     /locus_tag="PB780_000617"
     CDS             complement(7922..8329)
                     /locus_tag="PB780_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HDE0732691.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(8595..10070)
                     /locus_tag="PB780_000618"
     CDS             complement(8595..10070)
                     /locus_tag="PB780_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HDE0732692.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(10060..10908)
                     /locus_tag="PB780_000619"
     CDS             complement(10060..10908)
                     /locus_tag="PB780_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HDE0732693.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(10945..11307)
                     /locus_tag="PB780_000620"
     CDS             complement(10945..11307)
                     /locus_tag="PB780_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732694.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(11308..11466)
                     /locus_tag="PB780_000621"
     CDS             complement(11308..11466)
                     /locus_tag="PB780_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732695.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(11607..12231)
                     /locus_tag="PB780_000622"
                     /pseudo
     CDS             complement(11607..12231)
                     /locus_tag="PB780_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(12228..12539)
                     /locus_tag="PB780_000623"
     CDS             complement(12228..12539)
                     /locus_tag="PB780_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732696.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(12583..12876)
                     /locus_tag="PB780_000624"
     CDS             complement(12583..12876)
                     /locus_tag="PB780_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732697.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            13070..13717
                     /locus_tag="PB780_000625"
     CDS             13070..13717
                     /locus_tag="PB780_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDE0732698.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            13778..14923
                     /locus_tag="PB780_000626"
     CDS             13778..14923
                     /locus_tag="PB780_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDE0732699.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(15004..15309)
                     /locus_tag="PB780_000627"
     CDS             complement(15004..15309)
                     /locus_tag="PB780_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HDE0732700.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(15334..15765)
                     /gene="ruvX"
                     /locus_tag="PB780_000628"
     CDS             complement(15334..15765)
                     /gene="ruvX"
                     /locus_tag="PB780_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDE0732701.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(15762..16028)
                     /locus_tag="PB780_000629"
     CDS             complement(15762..16028)
                     /locus_tag="PB780_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HDE0732702.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(16283..17008)
                     /locus_tag="PB780_000630"
     CDS             complement(16283..17008)
                     /locus_tag="PB780_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HDE0732703.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(17369..17439)
                     /locus_tag="PB780_000631"
     tRNA            complement(17369..17439)
                     /locus_tag="PB780_000631"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17405..17407),aa:Gly,seq:ccc)
     gene            17556..18308
                     /locus_tag="PB780_000632"
     CDS             17556..18308
                     /locus_tag="PB780_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDE0732704.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            18324..18803
                     /locus_tag="PB780_000633"
     CDS             18324..18803
                     /locus_tag="PB780_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDE0732705.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(19054..20634)
                     /locus_tag="PB780_000634"
     CDS             complement(19054..20634)
                     /locus_tag="PB780_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HDE0732706.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(20627..21370)
                     /locus_tag="PB780_000635"
     CDS             complement(20627..21370)
                     /locus_tag="PB780_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDE0732707.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(21546..22322)
                     /locus_tag="PB780_000636"
     CDS             complement(21546..22322)
                     /locus_tag="PB780_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDE0732708.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(22570..23406)
                     /locus_tag="PB780_000637"
     CDS             complement(22570..23406)
                     /locus_tag="PB780_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HDE0732709.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(23417..24346)
                     /locus_tag="PB780_000638"
     CDS             complement(23417..24346)
                     /locus_tag="PB780_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDE0732710.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            24538..24891
                     /locus_tag="PB780_000639"
     CDS             24538..24891
                     /locus_tag="PB780_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDE0732711.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(24965..25621)
                     /locus_tag="PB780_000640"
     CDS             complement(24965..25621)
                     /locus_tag="PB780_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDE0732712.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            25847..27781
                     /locus_tag="PB780_000641"
     CDS             25847..27781
                     /locus_tag="PB780_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDE0732713.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(27867..29090)
                     /locus_tag="PB780_000642"
     CDS             complement(27867..29090)
                     /locus_tag="PB780_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDE0732714.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(29084..30982)
                     /locus_tag="PB780_000643"
     CDS             complement(29084..30982)
                     /locus_tag="PB780_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HDE0732715.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(30976..32094)
                     /locus_tag="PB780_000644"
     CDS             complement(30976..32094)
                     /locus_tag="PB780_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDE0732716.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(32177..33010)
                     /locus_tag="PB780_000645"
     CDS             complement(32177..33010)
                     /locus_tag="PB780_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDE0732717.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(33003..33920)
                     /locus_tag="PB780_000646"
     CDS             complement(33003..33920)
                     /locus_tag="PB780_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDE0732718.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(33943..34122)
                     /locus_tag="PB780_000647"
     CDS             complement(33943..34122)
                     /locus_tag="PB780_000647"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732719.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(34147..35271)
                     /locus_tag="PB780_000648"
     CDS             complement(34147..35271)
                     /locus_tag="PB780_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDE0732720.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            35454..36362
                     /locus_tag="PB780_000649"
     CDS             35454..36362
                     /locus_tag="PB780_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDE0732721.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            36503..38116
                     /locus_tag="PB780_000650"
     CDS             36503..38116
                     /locus_tag="PB780_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDE0732722.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT        11703 a         7708 c         6344 g        12914 t
ORIGIN      
        1 ggaagagact gtgaagtcaa cgttttgacc tgattattag agaaacaaat ggcaaaatag
       61 ttcttcatat ttcgccgttt ttcgagcgaa tgccgagggg cagctgattg aacatcgttt
      121 atttggaaaa agggattgtg acaagacgtt tgtcacaatc ccttttttta tatcttatta
      181 attcattctt ttgttctatt cttcttcttc cgccatgaat gagaaaccca tatcaaagaa
      241 ctataaaggt tatcaaaaca gcgtttatga ctaaatgaat tagagttatc tatcactatt
      301 tttcaagcga tttaaaccaa atggcataca atatggcata caaaaacttc aataagccgt
      361 gggggttttt aaatttcttc ttgttcttta aaatcaatga gtggagctac ataatttgaa
      421 tccacttttt caaacgtcac tttttgcatc gacgaatgtt ttctttccca agataacgta
      481 aatagatttg actctggact tataaagtct acttcacctt ctagtattac tttcttatgc
      541 aaatattcct ctaataaata tagacttgct aaagcataca acacattttc caaatttgct
      601 tccttataat ttgaagaacg accatgtttt actctgttat atattttcca ccaatctggt
      661 gtatcttcag gccacccatc tccaaaaggt tttagtgaag tcaatgttaa agaattcata
      721 accctaacac cattatccaa acacattaat tcgctagatt taataacagc tttaaaatca
      781 gttatgcttt tattgctact gctatcaaat ccgcaaagtt ctttgaaaat aatctcacac
      841 tccgaaccaa tcgctagtag tagtttttga tattcaatag agaatgtttt gaaattattc
      901 tcatcgagca tcacaaaatc tgtggtatta ataaactcat tttctaaatg tagataatac
      961 ttccaataaa aatttgtaaa ctctgctttg tccattaaat cacctctttt gaattataac
     1021 aaaaaaaaca cgtccacaac agacgtgttc tttgatttct acacaatcag cttgccaaaa
     1081 ccatccaatc agtcctagca tgcctgtatt aaaattgaac gttcatcatc tatcatcttt
     1141 cttagtccag catgtttgca accacaccta caattttcag tccaacaata gatttcgact
     1201 agctaagtct tgcaaaattc aggcagctaa cccttcattt actaccttaa ttatactaaa
     1261 tacccacaac gtttacaagt gtaatcattc taagaaatct gtttagctat gatcgttgta
     1321 taatctttaa acgccgttcc ttcattgatt gaaataattt tataactctt gtttttccaa
     1381 gctactttca tatcgtttgt tagttcataa tcttgatcgt atcgaataat aaacgtcaac
     1441 gttccttcaa gaatggtacc tacactagca agcacgtctt ttagcatttg tgaacgcaca
     1501 ttagcccaac attcaaatac agtttcttca ctatccacca tctgcccatc ttcatctttt
     1561 acccgttttg tctttttaaa cttgactctc tcagtcaatt catttacatt cttaatgatt
     1621 ggcataataa gcacctctca tttgttgtac taatgcttgt accgttactg gtatccgttc
     1681 gctgctggct tcttgacggt tcaaatacca atgttgagta agtaatgaca ccgccaaatc
     1741 aaattgctga taattatcca tatctttatc cgttgcgcta ctatcaatag cactttttat
     1801 atattcgcct gctgtatcaa tcaattgttg aataaagtca tcatcaatag tgtgatcgat
     1861 tcgcatactt ttttttatgc tatccaaaat taccatttta tctctcctaa tgaaaaaaag
     1921 gcagtgggat aaaccgccgc ccaatatttt tctatacttt tggtgttaca actggcgtaa
     1981 actcaatata cactgaagca ttttgatcaa ttttcttata atcgttgcga acgatcactg
     2041 caagtccttg tgagtagtaa tcgaattttt cccattgagt cgttacttga tttctacgag
     2101 caacaaagat agattgtgca atatccccca taatcatagg gaatgttcca gctttaggat
     2161 tggcaaacaa tgtatctgca atcaatacta ctggcatacc gaataatgat ttaccactag
     2221 gtgctgtcac atcgggttgt aaaatgtaac gtccatctga atctttcaat gtatctaggt
     2281 ggtaatatcc gctttggttt agaattacca ttttatttaa tgctgggtct aatgccacat
     2341 tgtatagttg tttcaaatcg tccaacgtag cagccgtttt cttagtgaat gtttttaata
     2401 aatccatgat atgcttattg tcagtgtttt ctaccaattt cgctaactga tctttgacct
     2461 cttgtacaat attcactgct gaatcttcca caacctcatt tgataaggca atcttaccag
     2521 cacgagtttc tactttataa tcaactgaag taaacatttc agcgtcaata tcaccaattt
     2581 cagcaagttc agcttttgtt gctaacactg cttgttggtt agttgctact ggatatttac
     2641 cttgtccatt tgatactgtt tttactgttg catattgagc taagttataa tttgaacgtt
     2701 tcaaatcaaa gacttcaccg attacttctt cgggaacaac tgccgctgca tttacagtag
     2761 tgactccatc acgcacttcg ccttgtgaac ggatatattc ttcataccca cgcaattctg
     2821 ttttttcgtt atcgattaaa gttttttcca tgttattact ctccttttgt gctttagaag
     2881 tggattctaa gctattgttt gtttgttttg cttggttgtt actcataaac gattcgtaag
     2941 aacgcttatt tacttggaca tttgatgaat cataagcggg aacggtcacg acactaattt
     3001 cattaagtgc tttgatttta tctattgatc gttcaattgt gccatcttct tttttgtcga
     3061 atgaatctaa tccaagcacg aacccaaagg acatagaatc aacaacccct tttgagatat
     3121 tctcatacac atcatttgaa taggtcgtgt cggttagctc tgcttcaaaa tgtaatcctg
     3181 tatcatcgac attcaatttc aacgtacctg ctttgacact agctagtggc tttgaataat
     3241 cgtgtccata taataagaac acatttgata aatccacctc ttttaatgct tcgggtgtaa
     3301 tcacttctac aaagccgcct aaatcttttg acggttgccc gaatttcaac gcatagcctg
     3361 aaatagtttt gccgtctgtt ggttgttttt cattttcttt ctctactttg ggagaatcgg
     3421 ctgttagctc agcttcttct gtcagccgtt tttccttttc ttcttcatta ttcaactggt
     3481 gtaactcctt cctgttcttt taattgatat ttctccaacg tatctaaata cgtaaagttc
     3541 aaacttgcta gtaaacgatc gccaccatcc atggggggta gccccatttt cgaccgcccc
     3601 tcattaatcg tgagaagtga ccctttgacc tgatccaata cattttttac ttttgtttca
     3661 ggatcagtct ctagtaactg atcggaatta aagcggatat tggtttcaag tttcgtatct
     3721 aattcactca caaacacatt aaagtaatga atcagcgtac tttggatata ctggagatta
     3781 ctttgaacgg tgctagaatg ttcattttca acgcctaaac gctcaatcgg tacaccaaac
     3841 gccttggcaa tttgttttgt gttccaatca ttggaattga taagcttcaa aacatcggtg
     3901 tttatttcta atgttttgta gtccattgtt tcatcaagaa tgatcgttct aagagcatta
     3961 tctccactac ttgaaccgtt ggcttcttca aacttttcac gaattgcgtt ctttgctgat
     4021 ccgtctaaat ctgacttatg aaccttcaaa atacctgacc cactgacacc acgagtaaag
     4081 aaattgtgca atgtgcggtt gccagcttgt tgaattttca gttcatcacg taacgcataa
     4141 agtggtggta atcctgttaa accatcttgg gtgaaatatt tgaagtgcaa aatatcgcta
     4201 gacttcacac gtcttttttt atcaccaatc tgataagaca acgtaccatt atctaattga
     4261 gtaaccgtta cacttgagtt aggtaataag tgaatttctt ctaccttttc accgttgcgt
     4321 ttaatctctg caaagctatt accgtttagc aacatattga ccgccaaagc aaactttaaa
     4381 tgccaaccat ccatttctga attaggtttc tcgtttagta acttcaccag ttcattatca
     4441 gcttgtggca tattgttttt gaccaattga atcggacttg aagcaatatc actagcaata
     4501 attcgaacgg ctgcaaacac atcactattt cttaatgcac gaacgcttgt aaagctggtg
     4561 gtgtaatcat ccgattggat tgataccaca tgttgtagga aaggatctcc cgttgtattt
     4621 tgtctcgctt taaaaaatgc catcttttaa cctcccttct gttctgtatt tagcatgacc
     4681 gctaccccaa taagcactat gcccagcgct aacaagccat agtagacatt tgttagtaag
     4741 gtgatagcaa ccaacacaca caccaatcca agaagtaaaa gaacggtatg aatgtaatta
     4801 gaaagaaaat tcttcacttg tataaaattc attgtctgct ttcgctcctt ccgttgtggt
     4861 gaagtaatcc attgcaaaca cataggcatt gattaatgca gctattggat caattttgtt
     4921 actgttcttt gatttattaa tctgaatgcc gttgttatcc gtttttaaaa tagcgttgtt
     4981 cactgcatgg gtgagaatcg tatttttatt gtgaataacg ttgccttcat aaacttgttc
     5041 tctaaaagtt cgggtcggaa cgtttagagt aatcgttcct tgtcttactt ctaacattgg
     5101 gtagttggct ttttcagctt tactgataag cgaattagcg ttatacgggt cataacaaat
     5161 ccccatacat tccaaatcat tttcttgaat caaatcttga ataaactgaa atacttggtc
     5221 atagtccacg attccacttt ctaattgagt aatggaacac tcaccagctt tttctagttc
     5281 tcgataaggc aaaccgtcgc gcttttcttt gtcttgaagc ccatatttgg tggctacaaa
     5341 gctatgtgaa tcacaataaa gttcgccgtt atctagcggt acgatccatg aaacgctagt
     5401 taaatcatct gttttagata aatccactcc gatataaacg ggcttgcctg taatatcttg
     5461 tggttctact tcggttgcct gccagtcttc agcaatcata tagctgtcct cactcgcttg
     5521 tctccataaa ttgaagtttt tcaccaatac agaattaagg ttattttgtt tcaaagcaag
     5581 gttcacatca tcttgaatgg ttggaatcat tacttttttg atttcttcac tttcaaaaat
     5641 cggattggct ttgatccatt tctcttgatc gtgaatttct tcgggatcgt ccaattccca
     5701 tatcgctata aagtacctgt cggcttgttc ttcgcctttt agcacacgat ctaacaataa
     5761 gtattcctcg tacataggaa cgtttaaatc aagcccagcg gtactaataa cacacaataa
     5821 gccgttcttt tgttgggtca ttcctgactt aatgacgttg tacgttttac gagtttttgc
     5881 ttcgtgccac tcgtcaagaa ttgcggttgt tggtgcaaac ccatctagcg tgctggtttc
     5941 actagccagt gccatagcga acgaattaga gggcaagtgc tgaatcttag agttcataat
     6001 tttcagttgt ggtcttaaaa acttactaga cttgaccacg tttctaagtg aattagacag
     6061 catatcatag cccaatttag cttgtttcaa agcgttactt acaaacaata cttgccttgc
     6121 ttctgctggt tcttgttcca taatcaacgc attggcagcc atgccacttg cgatatacgt
     6181 tttcccattc ttacggctca tactggtaaa cgctcgattg aatcgcctgt aattcccttc
     6241 tttagtacgc cagccataaa gactgcctag aatgaatttt tgaaatccta acatcttgat
     6301 tgctttaccg tccgtagatg gtagcaactc aatgaattta atcgctttgg tggcttgttc
     6361 ttcatcgaac acataaggga agctatctga cttagaacgc tccaaatcgc ttatatgacg
     6421 ctcacacgct tggataattt tcttgggtgc aatcagctta cctgatagga ctttctcaat
     6481 gtaatcattc atgaattcat caactctttg aacgggtctt cgggttcatc atttttaacc
     6541 ttgttcaaag ctagttttga tcgtgattct aaggtcatac ctagtgaggt ggcttgtttt
     6601 ttcaaatcac tcatagcttg tgattgaacc cgaaccgctg gattggcttt ctttactccg
     6661 ttttcgcatg tgataaacgt acctgatttt cttatctcgg cttcggctgt tttcattcga
     6721 gcaaaggcta aacaataggc taccaatgaa ccataatctg cttcactcaa tggataatct
     6781 ttttttaatg ttggtacgat acgattccat tcacttctag cgctcgctgc catccagtca
     6841 gggggcgtga tatttattaa tggttgctgc ttgaacaatt cttctctagc gtccacacgg
     6901 attgcttttt cttcacttga tatattgcct tttgtatcgt gtaatatttt agggtgtccc
     6961 atgttgtcct cctttcagac aaaagaaaaa ggaacgtaag caattaagct cacattcctt
     7021 cgtttttcga tcagaatttt tctttatcta atttatctat ataaatgatt tttccgttct
     7081 gcatttgaat ggtcacttga ccaaaatgag gtaactcaat ggtgcgctgt ttccctgttt
     7141 tatcagtaat aacaattgta tttttcaata aatctcctac tttcattata tactttttca
     7201 cttgtgatta caagtgtaat cgttatttgt aattaccctt atgccttttc acaacggtta
     7261 tttgtaccaa aatgagtagg catcgattgt cagtaagttt cacactatgc ccccttaact
     7321 caaagggtac tcttttctcg tctttgtctt tcattggctg tcttggaatt atggtgtgca
     7381 tggcataaag aacgaaaatt agtttcatct aaccgtctat tccaatcatc ttttagttca
     7441 attttgtgat ctacaacatc agctttcctt atcaagcctt cttctaggca atcctcacat
     7501 actggatgat ttaaccgata caagtatgaa gcctttcgcc atgttctact ttgatagaat
     7561 tgaaaatatt taccttcgct ttgctttctt tcttcacgct taattgccgt ttgctttgct
     7621 ttatgcttcc cacaatactt ctgcctataa tcaatcaatg ttttacatcc agcatgatta
     7681 cattgttttt taactgccat agattaccaa ccaaagcgat ctcgttcgtc agaatcatct
     7741 tgttcccatt ttgatttgta ttcaatataa ggcgaaacta tcttcaagca cgttccgtct
     7801 ttaaaattaa ctttagtgat tttatcttcc tcaaaactaa taaagtctac atcttcttga
     7861 gtgtttgaat actcaatgtg tttctcacca tcgaagtatc taatactttc catatgcata
     7921 attattctac ctccaataaa ttagttatca attcatcatc tgttagtagc aaagccaaga
     7981 acttatcatg gatgtttaaa aacaaattat cctcattcga tgtaagctct cgttgtctcg
     8041 ctcgatcata taggttaagc agacaattaa attcatccat tagctcacct gtatcatcat
     8101 agttgccact atctaccaat gatcccacta ctagcgattg acaccacgct actgactcct
     8161 gcagccaatc aggttcttct tccattatct ttaatacttc tggatcaaat gtggaataat
     8221 cttctgttac caatcccaca ctaaatgcca gcgtgttcat tagaaaccga tccatttcat
     8281 ctgtatattg aaagttcggt gctttgtttg ttgaaataat attgagcatt tactttcctc
     8341 cttgattcgt aacacgtggt aacacctacg taacaggtac ccgttaccac tcaatcccaa
     8401 cggggacaag ggttctagcg ttttggtaac aggtaacggg taaaatccag taacattatt
     8461 ccccagcata ttagctaact atttcaaaag ttttaaggaa ataaggtgtt accccgttac
     8521 caaattgctg tatcccttgg ggcagtaagg gtaaactagt aacgggctag gtgttactga
     8581 cccgttacta cccgttactt tttgtacttt ccagctcgat ttttattttc ataggcattt
     8641 gacttattgt aaaatcctcg tcgtttattt ctttttggca gttgcttccg ttcccaacca
     8701 ggaatactgc taataatcaa ggcaatcttt ttagtttctg catctgatcg accattcaat
     8761 aaatcctttg cttgtttatc aaaaacagct tccaaaatat cagcggtgag tacgctatca
     8821 actaaataaa actcttgttg catagtagct ccattataga aatgctgtaa atttttctca
     8881 ttacatttac tgtcaggata gttctgataa taagaacgtc tcgcccacgc tggagcttct
     8941 tcccatttat acgggatttc catttccaaa tatttagtga ttgcttcttt aattggattc
     9001 tccatcttgg catcctcttg atagtctttt gcaatttcta agacttcttt atcttcatcg
     9061 ggactaaaat agattcgttg tcctttatca aaaagcacct ttgcttcagc taacacttga
     9121 agaaaatatt catcctttgt tttaaacgga ttcatctttg gcttattctt acaaggcaaa
     9181 ggataaaaac ggcggtttcc tgtatcatct ttcaaatact gcccatcatt cgacgttcca
     9241 ataaatacac attgccttgc ccaagctatg acattccgac cataaggagg tctaatttta
     9301 tcgatcttgg cactgatgaa gttttttatc tgttcaattt tagttttgtt cattgaagat
     9361 aattctccaa gttcaataat tacattgcct tgaagctgca tataatcatc tttcttttca
     9421 cctagcgaat ctaatgaatc ggtgaaaaaa tcatccgtgt aaagtaatgc tggaacggta
     9481 ctcttaccaa tcccttgcgg tccatccaaa actggaacga tctcaaactt tattcctggt
     9541 ttatagatac gtgcgactga tccgactatc caacgttttg ttacttctcg tgtgtaaagg
     9601 ttgtcgtcta cccctaaaag gtcaataaac agtgtttcca gtctaggctg ttgatcccat
     9661 ttaacttttt cgatacgttc ttttactggg ttgaaaacat tctttctagc cacatttact
     9721 actgctttgt gtattttgtt gtcatcaaaa actacattgt agttttcttc aatatatgcc
     9781 gttaaggcat cctcaaaact gtcttcccat tctccagcgg tacgattgat ccaaggagaa
     9841 tcttttaata ggtgaatgta tccgctaaat tcgttataag caattgttcc agctaaattg
     9901 ctatcattgg ttaaaatcaa ttctgcattc acaacactgt tacttttaat ctgatcgtct
     9961 ttattgcatc ttaatcgtga catccaggtt ttcaattcaa tgatattgtc ttcacgctcg
    10021 ctggcttcgg gaaagtctaa aatactatct aatttatgct tatgagccat gagctttccc
    10081 cctcaaatct tttttaatca tcgatcgaat aatttgattc acttctttat cttttagcgg
    10141 tggttcattg tacgcatttg ctgtataaat caattctgct aaagtttgat tatctacacc
    10201 gtgccatagt aaccaaccgc ataacttagt taaatgatcg tttctactac cttcgccagc
    10261 gccttgaaca atcatatcta atttttttgc tgtccctgtt tttgctacgg gtcggtattg
    10321 agtctttatt tcttgcggtt tttttagcag taggttcact agccaatcgg gagctggtgc
    10381 aatattattt agagatacgt tttcaatagg ttcatatgct ctcccaacac ttcttgaaat
    10441 aatcatagtt ggcgctattg gaactccttt agtgataata tcaatcccgc ttaaactttt
    10501 acctttatca tcacgcccga agatatcagc aaatgcacct gtttcttgtt ggataattac
    10561 accatcagga attttaaaga agaaatggat tcctttacct ggtgtgattt ccgtataagt
    10621 agaaggaaat ggatcataca ttgaagcaag ttttttataa ttctcaacgc cgtttcgtgt
    10681 ttcagaatgt tgatctaggt caactaccac catttgatga tcttcaagca ttaacccaat
    10741 atttctagtt gcatcttccg tccaccattc attaatttga ttagaatcgt tagttgcatc
    10801 attccaacca ctagagcctt ttattggaat tttagttcca ggagataagg gataaattga
    10861 ccatcccgac ttttgtaatt ccgttgctgc ttgataatta tttgccaata aacctctcct
    10921 cctttttcaa aatactggta taaatcattc agacacctcc atgagcttca actgctcaag
    10981 ttcgtatttc aacccatccc gaacaaattc agcacctttt aattgattct gatactttac
    11041 ccatgtatta aatgttttca cccttgcttc ttgcatatca gtaatgttta accatgcggt
    11101 taaaatctga ttttttagct cgttgatttc tcgacaaatt attttgtgtt ctgcatgttt
    11161 tttgagtaca tacaaaactg ttagtttttc attgaatgaa tttccaaagt aggttttccc
    11221 gatattattt tctagttcag cgatcccttg attaatttcc tctaattcaa ggctatcgag
    11281 ttggtttaat agttttgttt tgtacatcta cttatgccct ccagttctta attgatagtc
    11341 gtactcgtca taagcagtga agagatataa cgctaatcct gcaaagatag ctatttttgc
    11401 aattaatggt aataagccga aaattccgcc gattaaaatg cttcctataa ttaagtttcg
    11461 tttcataatg gttccctccg attttttaat atggtataat cggaacaaag aatatcgggt
    11521 tttatcccaa ttaagttgcc tttatcctgt ccgccaagac tgttatggat aaggcttttt
    11581 ctttgttctc gatcatgtta atgctcctaa gttgtctgca attcagcaga acgattataa
    11641 attttctcac tcactgaaaa ctgcaaccca tatttttctt taacagtcat taattctact
    11701 gtctcatcta aaataggttc acgatcaacc aacatttcag gagtcatatc tttctttttt
    11761 accattttgg aatgaccata aatagttgat acagctttat tggctatcac attcgcttta
    11821 atgtagtcta ctggttttgg ttcagtaata gcgtggctta atttagtcat tgcttctttt
    11881 tggtgttctt tgtctagcat gcggaatact tgaaagcctt caagtcctgt ggactggcgt
    11941 agttctttga tgatgtcgaa aatccagtct tgaaatgcct cggcttcttt tttacggctt
    12001 ttgaatgcca tgcggtaaat attttttttc gttgatgatg tttacatctt gtgttctgcc
    12061 caatgaatcg atgaccttac tagtagtaac cccatcttta ggcaaagagt gaatggctct
    12121 agtaacctgt ttaagaccta acgcttccgc aacatctttc gccactgccc accattcatc
    12181 attaatatcg acaaatctta taatgtgtcc gttccaaatt tcagttttca ttcaacgtcc
    12241 tccagttcaa gccctagcaa gctcgctaac aactcaactt gctgatacaa aatttcttga
    12301 tactcttcaa tagaaagtcg ttctttaaac tctgcttgta ctaaatcgtt caagccacgc
    12361 aatactccgc ttaccgttgg ttcttgtggt agcaattttt gatagtccat ttgttcgtac
    12421 aaacattcaa acgtttcttc tagcgctttg ccttccaagt ttgctaattc tgccgctgtg
    12481 tcttctgtaa gaattgttga taatactttt tcgattgctt tattttgtgt cgattccatt
    12541 attatttcct cctattaatc cctttgcccc cacggcttgc gtttattttt ggtattcttc
    12601 cataaagcga tcacattcat cttgtgaaat tcttacgact ccatcaacta taatcaactt
    12661 taaaccgtta ggaacatatt tctgcatcat agtattgtat gaaacgttca tatacttagc
    12721 agcttgtgcc ttgttcatat attttggaaa ctggtacttt ttttctaata atgaaagtaa
    12781 cgttccttga gtttgctcta cttgttgaac aactcgatcg tcaataattt taaaagctgg
    12841 atcatcaagc gtgagtttgt caatttttaa ttccatattt atcccccctc gttagcatac
    12901 atttctatac aaagatttta aaaaaatgca agaggctctt gaatacattt ctatacctct
    12961 tataaactca atataccatc ataccttttc atttgcaaga attttaacat acttttttga
    13021 acttttatgt ttcacacata tttaaatcgt gatatagtag agaaaatata tgaatggtgg
    13081 tgaaaatgtg gctagaaaaa aataccccga agcagtggag acaggacaaa gaattagact
    13141 ggctagaatt gaaaagggac tctcaatgga acaacttgga ggtaaattat ccccaccggc
    13201 cagtaaaggt gccgttagta attgggaaaa tggctataat cttcccaata acaccaggct
    13261 taaacaactt tcaaaggtat tagatgtatc cactacctat ctattaaacg gatcttatac
    13321 attaagagat atgcatatga tgcccgaaat taagcgaaat gtgcttgaaa agcaattgaa
    13381 aaaaactatt gaaaaaaata actctataat tgattctaat ctttctaatg ctatcaataa
    13441 catcgaagaa gataacctga ctgtctcaca gaagatttat ttaacaaacg caataaaata
    13501 tcttaaaaca ggatctcgaa ttgaatttcc tgacgggagt accccaattg cttatctcgc
    13561 agctcttcta aatacgcttt tacgatatga ctcattaatt aatgattcag aaaaaaatac
    13621 agatgagaaa aaagagtatc aagaatttta tgaaaatgat cttctaaaag agttcacagc
    13681 tcttgttact gagctaaata aacactattc aaaataataa taaactgcct cctccaaccg
    13741 cccccacggc ttgctacact atggagggaa aactattatg gcaacattca agcaatatga
    13801 aactaaaaaa ggtaaatttt ggctctatga agcctatctt ggaatgaaca aaatgactgg
    13861 caaacctgat aaggttcgta gacgaggtta caaaacaaaa aaagaagccc agcttgcctt
    13921 gtcacgcttg caagtggact acgataaaaa cggtattaaa aaagcgaaca acgaaacgtt
    13981 taaagaagtt tatgacttgt ggctagaaac atataaaacc actgtccgag aagccacctt
    14041 catgaaaact gaaatcaagt ttagaaagtg gattcttcca aaatatggaa atcttcgtgt
    14101 gaatgaagtc acggtaaaaa aagcccaaca aattgtaaat agctgggctg aaactactga
    14161 ccaatacaag gtacttcatt caacttctaa gcgtattttt agctacgcta tcaatcttgg
    14221 gattattgag actaatccac tagacaacgt attaatgcct aaaagaacca ccactaagca
    14281 taaagaaaag gtcaaagttt acagtaaaga acaactgatc accttgtttg attacctaaa
    14341 cagcaaacct gaaacttatc gaaacgactt tgataaaacg ttactacgct ttctctttta
    14401 tagcggggtt aggatcagtg aagcccttgc attgaattgg tcagacattg attttcaaga
    14461 aaatacggtc accatcaata aaacactaag ccaatccaaa aatggctata aaatcagtga
    14521 tcctaaaact gattgtagtg ctggggttct tccactagat gaaagaacaa tttctatatt
    14581 aaaaaaatgg cagattaatc agcggaaata tatgctgaca cttggtgtaa ctgatccgac
    14641 tatgattttt tgtggtatct ataaagaact aatcacacac cacgctattt atgcaagatt
    14701 gattactatt tgtgaaaaag ctatggttcc atttctcggt gtgcatgtag caagacacac
    14761 tcatgcttca atgctcttgg acgctggagc tactatggta gaagttcaaa ctagattacg
    14821 gcatacaaaa attagtcaga cgatcgattg ttacggtcac ttggcaaaag aaacgaaaga
    14881 aaagactgtt gaaaaattag tacaacactt aaatattgac tagatggcat acaatatggc
    14941 atacagtaaa aaataaaaaa aggagaaacc ctatcatatc aaggtttctc ctagctttat
    15001 agtctattct tcttcttccg ccatgaatgt attgaatact tcttcaatca tgtcccattc
    15061 tgcgtctgtt tcgatttgtt ctaactcgcc ttctgttcca tcttcgtttt cgatgtatgc
    15121 ataagcttgt aattctacat cttcatcttc tgaaacacca gcaggatata aaagaacata
    15181 gttacgtcca aattcttctt gtccgtcaac tgttagtagg atttcatata atgtttcatt
    15241 tccttggtca tccaccaatg tgatgtgttc gtgtccttca tggtcgtggg aatggttatg
    15301 ttctgtcata agattacctc ttttctttta ttattaaaat aattgcttac ctgagccgtc
    15361 taaataattt tgtaagatca tcacagctgc taatttatcg atgacttttt ttcgtttggc
    15421 tcttgaagta tttgcctgct ctactagcat acgttctgcc tgaacagtgg ttaatcgttc
    15481 atcttgataa tcaactggca aggagaatag ttctttgatc ttttctccgt atgacatgga
    15541 agcttctgcc cttggtccga tggaattgtt catattttta ggtaatccaa ctacaaaccg
    15601 gtctacttca tattgtgcta ctaattcctt caaccgatca aagccaaact cttcttctgc
    15661 ttcattgatc cgaatgatct ctactccttg agctgtccag ccaaaggggt cactgatagc
    15721 aacacctact gtcttagaac cgacatccaa gcccattact ctcataaatt cactccatga
    15781 tttgagaggt agtccttgac aattacttcc atgatctcat cgcgttcgtg acggcgaatc
    15841 aaatttcgag catcacgata acgaggaatg taggccgggt ctccagaaag caagtagccg
    15901 acaatctgat tgattggatt gtagcctttt tcttccaaag cccgataaac aatcgctaat
    15961 gtttcgctca cttctttttt gcgactatcg tcaaaaccaa aacgtacggt ttcatctgta
    16021 aaacccatga ttctacacct ctttcccgca aggtaattcc agcttacctt ctacaacctt
    16081 attttactcg aagaaaggga aaactacaaa caaatattcc aaacttccga ataaattaag
    16141 aaacactatt tgtatgtttt tttccttttt tctgtagctt tatcaaaagt actcttttca
    16201 taaagtatac gctatatttc tttgaatgta tgtttttaca caattttttc ataatattca
    16261 taaaaaattc gaatagctgc tactaatttt ttgtttttgt tgcttgtcct gtctcataat
    16321 tgatcgtgta gccatcttga acattgtaga taaagacatt gaattcaatt tttttgtctt
    16381 caatgctttc tgcttccaat tgcactcccc tggctaccag ttcattcccc tcaaaatgag
    16441 gagtgacacg gtagcgtaca tgatgatttg tttctttgat atatgccgca atatcatttt
    16501 cgtgagcaag catgtatgga gcattcaacg aacgtgtccc tgtcatcaag tttttaatgt
    16561 tgttattttg accagtcagc tggaaaccaa ttaaatggct gcgattatat aaccattgcc
    16621 cgtcacttaa tttcttttgt ttccaacctg tcgggtcaat atacaaaggc tctctctttt
    16681 cagtagggaa caaatctttt ccaagcatag cattggcttg tcctactctg ttcaatttat
    16741 caatatttga atagctttcc catgttcctt tggaaaggct aagatcctct tttgtaaatt
    16801 ccggctgatt cccattgatt tctatttctt gatgttttcc atcatattct ggcacatccg
    16861 ctacttgaga agcttgtggt gtggaatcaa aaatagttgt aatctgctga aagatatctg
    16921 aatctttttg tgtggtggaa acaccgaatg cccctgcaat cacagcagcg acgatcccca
    16981 cgatcgcctt ggttgttttt ttgctcattt ctttactcct ttactaacag ctccctaaat
    17041 cttaacatat gttcccctat aaataaatac tagaaagaaa tagaaacagc aaaaaataaa
    17101 acctgtttta agatggctta aaaatacaaa aaagagacgg attcctcgtc tctaatcagt
    17161 caccactcta tctgtaaggt atttattatt attcaaaaat tttcttcata ctcaagacaa
    17221 tacactgatt tcacaaccgc ttggtaaacg gtgcgcaaaa gcttgcatct gcggtaataa
    17281 gtccatccaa gttaaaaaca taagaagttg tttttgcttg gctgttctta ttacttgttg
    17341 cagagcagct ttttcacaac ctattttaag cgggtgacgg gaatcgaacc cgcgacatca
    17401 gcttgggaag ctgggatttt accactaaac tacacccgca atacaaaaac agtatacgcc
    17461 tcttttattc cttcctcaag tttttttatt ctttacgctt ctggtatgtt ttgtagtaaa
    17521 ataaaactaa tcaatcaatt attggagaag atgctatgac gggaaaagaa ttagatctgt
    17581 tgctggacac ttgtttactc gctgggaaaa tcatgatgga aagcaatgcg gagatgtatc
    17641 gggtagaaga tacgatgagt cgaattgctt tagcttctgg aaatttccgt ttggtcagct
    17701 atgtcactca aaccggtctc tttattggtc ttgatcgtac ctcgaccatc cgaatggaac
    17761 aaattaccaa ccgttcgatt aatttagaaa aagtagtaaa agtaaatgat ctttcacgta
    17821 aatatgttgc ttcagagcta acattagaag aactttactg cgaactgcaa gcaatcgacc
    17881 gagaccgttc tttctttcct gtctggttgc aaatcgttag cgcggcagtg atcagtggaa
    17941 caattatggt gttatttggc ggaaagatga ccgatttacc tgtgacttta gtcattggag
    18001 gattgggata cgcagcttat ctatatagtt tgaaattttt tagaatcaaa tttttatcgg
    18061 aatttttatc ttctttattg attggcatag ccgctatcta cagcgtacgt tttggcttag
    18121 gaagcaatca ggatctaatc attatcggat gtgtgatgcc tttagttcct ggagttcaaa
    18181 ttacgaacgc tatccgtgat ttgctagctg gacattatgt ctcaggagtt tctcgcggaa
    18241 cagaagcgat gatgactgct acgatgatcg gttttgcgat tgcttttatt ttccagcttt
    18301 tttattagga aaggaacaca agtatgggga cgatatttat tcagttttct tttagttttt
    18361 tagcgactgc ggcatttgcc gttattacaa atgtacctag gcgttcattg atttgttgtg
    18421 gtctttctgg tgcttttggc tggatgatct actggatctg tgttcaaata ggcggatctg
    18481 ctgcttttgg ttcattgctt ggttctttag gagtagcatt catcagcgat cttttctcta
    18541 aacgtttgaa aatgcccgta acgattttca atattcccgg gatggttcct ctagtccctg
    18601 gtggtttagc ttatcaagcg gttcgaaatc tcgttactga atcctatcga gaagcggctc
    18661 attatgcggt ccaagcaatt atgattgctg gagcaatcgc tttaggtctt gtattatcag
    18721 aagtctttaa ccataacatc cgtaatttca aagtaaagcg tgacgcaatc atgatgaaac
    18781 gaaaaaaacg gctttttaaa taaacgaagt gtaacaaaga aaaggttgtg aaagagacgt
    18841 tcagccttga gcgatgacaa aatatctcac aaacattgcg acaagtaacc gcaggagcaa
    18901 taagaagtaa ttatgagtat taaaaaaagt tttgtaaaaa tagcttctca catttttaca
    18961 taaaattgac ttaatactga gaaactgact tttgaacacc ctctatctgt aaaaaagggg
    19021 ctgcgacatg atttttgtca cggcccctct tctttattca aattttttcc aaaacgttcg
    19081 ttgataataa acgatccata gagcggaaag tattagaact aaaccgatca caggaatatt
    19141 cactaaccaa aatggataaa acaaagccaa cattccgtat aagacgataa gaataatact
    19201 gatgatcaat gaacccatca tagttgcgac taaacccaaa cttcctgaac ctctgttaaa
    19261 taattggtta atattggtcc agttcagcag cagcaaccga taatctctag cgaaataacg
    19321 tagtgacaat aagtagcagc cgaataaaga accaaatagc aagctaggga taaaaaacaa
    19381 aggcagctga aaagacagtc cgcttaataa tgcgatccca ccagttaaaa aactctgcaa
    19441 tatccatccg aatctgaact tttcttttaa atattggttc atagagattg gcaaagaacg
    19501 gataaacaga aaattttctt gatccaaaga aataagattg ctgataaaag aagtctgatt
    19561 aactgttaag aaagccaatg cgatccctac caggaaaaca acaccgatta acttattatc
    19621 taaatcagtt aattttattt cgcctcctag ggcaaaagca agaataaaaa tgattggagt
    19681 taacaaggaa gaagaaagaa cttgcataat caaattaggt tcttttacta attgagcatt
    19741 gtaactaaat aaaagctgat gcacattttg gttcttttta tgggttcttt ttattttgaa
    19801 ctcactgttt gaatcagtca gttgctctgt aagctttggt actatcagct gttttacagc
    19861 aaggaaaaat cccagagccg tgatagctag tattccccaa cccaataaac cttgttgaga
    19921 aagtggctga ctggcagcat catagaatgg caataatatc gaaatcggat gccgatctgt
    19981 taactcattt tcaatagtcc ccgtttgatg attcatccac atgattccag caaatgcgat
    20041 cagcatcgag ctgccaagca agatactaga aactgttttt ctatgttttt taaacactgc
    20101 tgttcgagcc aaaccaaaaa caatcaaact agccagacaa aaaagaataa tcaaaaatag
    20161 agaaaacaaa aggattgaga taatgatagt aagcaaaatc cctagatcag ctcgccatcc
    20221 ggttaaaata aacatgacta gaagcggaaa aacaaaagga atgatggtca aagcaactac
    20281 caaaatcttt gctgaaaaaa cctgttcttg tccaaaaggc agaggcaaaa atacttgcag
    20341 gtcatttcct tcaaaaaaga tattataaat gactgatata ctttgagtta aacctaacat
    20401 accaaaaagt gccacgtaat aagtaaagaa acctggcaat ttaacaaagt tcatagcaaa
    20461 catagcaaat ccgtaaatca gtaaaaaaat cagccctgaa aaaagatttt gagacaaaag
    20521 atagatagtt aatcgctttc ccttttttcc tttctctctt gctttattcg tgagctgtgg
    20581 attaacatat aaaagattaa ctcgaattaa ttctaataat tgtcgcttat acatacggtc
    20641 catctcctgt cgcttgcctt ccagccattt tcaaataaat cttttctagc gtttcatcag
    20701 gtgagccatt caataattca ctaactgccc cctgataaat caattcacct ttcttcaaaa
    20761 tagccagttt atcgcataac tgttgtgcgg tatccaatac atgtgtagaa aaaatgactg
    20821 tttttccttt tgcggcatga gccttcatca tttctttcaa atcaaatgct gcttgaggat
    20881 caagtccttg caaaggttca tccaataccc aaatatctgg atctggcagc aacgtgccga
    20941 tcaaaatcgt tttttgacgc attccatgtg aaaaacttgc gattgtttca ttttgatgac
    21001 tatacatatc aaaaagcgct gtcaattctg ctaaacgctt ttcttttttt tgtgtgtcta
    21061 attcataggc agctgaaatc aagtcccaat attctccagc cgtcaattgt aaaaaaatat
    21121 ctggggaatc aggcacataa ccgatttttt gtttgatact caatcgattt tccgtcaatt
    21181 tcattccatc tactaaaatc gttcccgatg atggttcaat aatactaact aaacttttaa
    21241 tcgtagttga ttttccggca ccattatgtc ctaaaaatcc aaaaatttcc ccttgtctta
    21301 tcgagagaga caagtctttt aatgcttgtt ttttcccgta acttttactt acattgatga
    21361 attcaatcat ttctgtactc cttttgtttg ttattacttt ttattgtacc cttcgttatt
    21421 ggtggagtaa atgtcaaaaa gagtgcataa caaaaaagga tagaaacgac taggctgcta
    21481 cttgggttgt tctcttctac aggaaaacac tcttctaaca ggtaagatat gagtctttgt
    21541 tttctttaaa atgattcatt ttctatcttt tcttgcataa aattctttag tttatcaata
    21601 cttctctttc ccatctctag ttgttctcca tttgcaaatg ttactatttc gtttactctg
    21661 ctaatagatg taatgttttc agtattaatg atataagctt ttaaatcttt acataaatac
    21721 tcatattcga gtgatttttt atatttttta attgtgccgt ttacgagaat ctcttcatct
    21781 ttcaatacta ggcctatttt atttttataa cctggcatgc tcattagata aaggatttca
    21841 tcgtaatgga tatagtgatc gctattacca taacggacag ttgtccacct ttttttcgta
    21901 gcatgttgaa caggatagtt ttctttttct ttcaataagt aactttggat gcttttttta
    21961 tttttttcat aagtgaattc ttttatcaaa taaccactag gcgtaatctt tcgattaata
    22021 acagttattg ctttattcgg atgagacgtt agataaacaa ttttacactt ttcattcttt
    22081 tctctaagca tttcgcctag ttcgataccg ctataatagg cgttcaaatc gatgtctagg
    22141 aaaaaaatat ctgaggaata gatttcatta tttttaatga attcaacaaa ttgtataacg
    22201 ttcaatccct gaacgatctc aaatttccaa ggacttaata aaattctgga ttcttccaaa
    22261 atttctacta gcattttttc aaaaaccaaa ctatcatcca aaataaatat acgcgttacc
    22321 ataaaaagta cctccactcg atattttact atcaaaatca tttcatacaa cttttcaaca
    22381 tacatgttag gaataaattg taattctctt cacaatcttt tccaacacct ttgaaaccga
    22441 tatcaaatac tttcaggatg ctgcttctaa aataaatatc aaacaaatga atacatcatg
    22501 caaaaaaagc cagacaaccg acgtccttgc gtgtcagctg tctgagctat gtttatttat
    22561 tttgttcttt taaaaagaac gatacacata ctgatttttc ggagcagaga ctttctcagc
    22621 gtttgtaaca actacagatg ggatttcacg cttgaatggt tgataatagt ctgattggat
    22681 ggttccttct attttatacc attcatcatt tttaaccgtt actccttccg gcatatgcgt
    22741 caacaaacca aagactcctg aatctgccac acaatggata atgccgaatc ggaacaagaa
    22801 gatatctgta ttgctatcgt tcggtgtttg gtaaacaaat ccttcatagc tgattttttt
    22861 gccaatgaat tcgctagggt aattataaat tagctccata acttctaaat agttttcatc
    22921 tgtgatcttg atagtttgct tatccccata cttttctttc aactcattca tttggtctaa
    22981 gtagtctgat ttgttgaaat aaatactggt atccggcttt aagtattgtg tctgcatctc
    23041 cggatcccct actgattctt tacttactgg gaaattaaat cctttggctt ctacgatcgt
    23101 agtgtcgaga ctaactgttg gaaacaacgt ccccacgatt actggcaaag ctagcagtac
    23161 ataagcgatt ccacgttgat aaggttttga caacccatgg tcatgatcgt gctcgtgtgc
    23221 cttttcttct gcttttccgt ttttgaccca caaatacagc tgaactaaag ccaaaagagc
    23281 agataaaatc attgaaagaa tagctaagta gcgataatgg acattgatat attgatcgag
    23341 tttgccagaa acttgcaagt acatcatcaa ttcgaaatac ccagataaaa tcaaaaaccg
    23401 cagcatattt tccctcctaa ataaataatg tgaatccaat aacgctaagg gcaatcagtc
    23461 cgatcagcga taaaataaag cgtcctgtaa aatatcgttt catcatcagt agatttttga
    23521 tatcgaccat tgggccaaag accaaaaagg cagctacagg tgctgtgccg aacagactta
    23581 acaaagaaga tcctataaac gcatctgctt ctgagcaaag agacatcgta aaggccaata
    23641 acagcatgat gataatggct aaaatctttg ttgagccgag cgttagcata attctagttg
    23701 gtaaataagt ctgcatcgca gctgcgatta atccgccaat gatcaaataa cgacctgtat
    23761 caaaaaattc gtcgattgaa tgtgtcaaca catgcccagt ttgatggatc agctgatttt
    23821 ttggttcatt cttctcactt tgttcttctt catggacatg atgatggtga ttttctgcta
    23881 ctttagacgt gattttttct ttcaatacag cgtctttttg aaaataggca agccagacac
    23941 caatcacaag ggcaacaaaa aagctgccta tcacccgcca gcttgccatc tgccaagaat
    24001 tgccaaatgc gataaaagtg gaaaaaatca cgattggatt gatgatcgga gcagtcagca
    24061 taaaagcaaa cgctgtgtga actggtacgc cttttttaac aaattgatga acgatcggta
    24121 cgatgccgca ttcacatgaa ggaaagaaaa atcctaagat cgaaccagta aaaatagaga
    24181 agatacgatt ttcaggaagc catctcttca cacgttctgg ggtcaaaaat acttgtaagg
    24241 cgcctgagat aagacagcct aataatacga aaggtaatgc ttcaataacg atcgataaga
    24301 aaatcgtact catctgcaaa accgaatttg gaataaaaga aaacatgagt ttcctccttt
    24361 ttatttctag ttcattcgct ttgcaaatat acgcttttat tcaaaaaaaa caagttgctc
    24421 tgtaaatttt tacacaactc ttatttttta ccaaagcact tcaattgact ttttctgtta
    24481 ttcccattaa attaaggtct catacatatc acttctatca taaaagggag aataaaaatg
    24541 aaacaaacca ctaacttgac tgaagttcaa gacatactgc aacagtcatc cgttgccgtc
    24601 atttatcttt cgatgcctaa ttgcagtgtt tgtcacgctg ttcgtccacg actagaggaa
    24661 cttctcacac attatgatat tccggcactt catttggatg catttgaaac accagaagta
    24721 gctagtaaat ttgaagtgct gactgcgcca gttgtcctga tttttcatca aggaaaagaa
    24781 gtctttcggc aagcacggtt catcgacttt aaaaaaatcc gttatctact agatgaactg
    24841 acagcagaag atgattccct ttcttatgaa gaaatcttcc gaacaaaata atcgttgtcc
    24901 aaacagaaaa aagcgattga acaagtgaaa gttttgcctt catttgttca atcgcttttt
    24961 atttttattc tgctgtattt tcggaatgcg catttgcgtc agaatgcaag aaatagatca
    25021 aggtttgcaa ttcattcgtc aaatcaacat tttggatcaa tacatctgac ggagcattca
    25081 atcgagcagc ggtaaagttc aagattcctt tgattcctgc ttccgttaac tgattgacta
    25141 cttcttgtgc tctttcagcg ggtatcgtta aaattgctac ttcgatttgt tggatcttga
    25201 tttgttctga catatcttcc attggataaa cggggatacc gtctacgatg cgcccaacga
    25261 tttcctcatt cacatcaaaa gcacaactga tacgtatgct attactttga tggaatttgt
    25321 attttagtaa agcacttccc aagttcccta caccgatcag cgcaacattt gtcaattgat
    25381 cgtcgtttaa tgtctttgca aaaaagtgca taagatcttc tacatcataa ccatacccac
    25441 gttttcctaa ttcgccaaaa taagaaaaat ctcgacggat cgttgcacta tctacttgaa
    25501 cggcttcact taattcagtt gaagacactt ttttctttcc agcattattt aaaatcctta
    25561 aatagcgata atacaaaggc agtcttttag ctgtcgcctt tggaataata tgatctttca
    25621 cgaaatgacc tccacgtatt gtctaattac atctttgatt tctccatcat agcagtattt
    25681 ataggaaaaa atcaattcca ttgctcagat attgtgaaat tttattcttt attcatttat
    25741 ttatcatttc tgcaaatctc gcagtataat ggaatttatg gtagactact aattgaaaaa
    25801 tagtttttta tttgaggaga attcacaaag tagaaaagag gattccatga tattattaca
    25861 agccaatcag attgctcgcc attttggtgc agaagtctta tttgaaaaca tacatttaga
    25921 aatcagtaca ggcgctcgtg tcgctttagt tggcagaaat ggcgctggaa aatcgacttt
    25981 attaaagatc attgctggaa ttgaagcacc tgatgaagga aagatcgcta aaaataaaac
    26041 agcgactctc ggatacctcg ctcaggacac aggacttgat tccaatgaaa cagtttggaa
    26101 cgagatgctg aaagcttttg aacaagttag acaaatggaa gagcggatgc gcgagctaga
    26161 gattttcatt agcgaaggta cgccagacac acctgcttat agttcttatt taaaagaata
    26221 cgaccggctg caacacgatt tttcagatcg aaacggctat ggttatgaaa atgaaattcg
    26281 ttccgtattg catgggttta aattcgattc ttcattttat gatcagaaaa tcgatacttt
    26341 atcaggcgga cagcgcacac gtttagcttt agctcgcatg cttttgcaac accctgacat
    26401 tttgattcta gatgaaccaa cgaaccattt agatatcgag acactttcct ggcttgagaa
    26461 ttatctgcaa agctatactg gtgcattatt gattgtttcc catgaccgtt attttttaga
    26521 taaagtagtc aatgaagtct atgaattaag cagaagaaaa atgcgccatt acaaaggtaa
    26581 ctattcgaaa tatctagatt tgaaagcaga acagttagca agtgaatgga aggcttatga
    26641 aaagcagcaa acagaaatcc ataaattaga agacttcgtt gctcggaacc ttgttcgcgc
    26701 ctctaccaca aaacgagcgc aaagccgcag aaagacatta gaaaaaatgg accggctgga
    26761 tcgtcctcaa ggggatgaaa aacaagcaaa ctttctattc agtatagaaa agccttctgg
    26821 aaatgtagtc ttgcaaatcg aagatgccgc tatcggatat gataatgtca ccttatctga
    26881 accaatcaat ctagatatcc gtcgtcaaga agcgattgct ctagtaggtc cgaatggtat
    26941 cgggaaatcg acgttgctga aatcaatcat tcaccagatc ccttttatcc gtggaaaaga
    27001 aacgctaggg acaaatgtca ccatcggcta ttacgatcaa ggtcaagctg atctgcatac
    27061 aaacaagacg atcctagcag aattgtggga tgagcatcct accactccag aaaaagagat
    27121 ccgaagtgct ttaggtgctt tcttattttc tggcgaagat gtagaaaaac caatccctct
    27181 gctaagtggc ggtgaaaaag cacgggttgc tttagccaag ttagcgatga acaaagaaaa
    27241 ttttttgatt ttggatgaac caacaaacca tttagatatt gacaacaaag aagtattaga
    27301 aaatgcattg atcgattatg acggcacact gctttttgtt tcccatgacc gatatttcat
    27361 caatcggatc gcgacaaaag tcgtcgaact ctctgaaaat ggcagcaaag tctatctagg
    27421 agactatgat tattatttag agaaaaagca ggaagaagaa gaaatcgccg aattgttggc
    27481 acaagatgag ccagaatctg ctcctgctcc cacgaacaaa tctgattatt accaaagcaa
    27541 agaacagcaa aagcttatcc gtagccttca acgaaaaatt actcaggttg aagaagaaat
    27601 ggcgcggatc gatgaatgga tcgatcagtt ggaacaagaa atggtcgcgc cagaaaacct
    27661 ggaagatcac gtaaaactca atgagctgaa ccaagattta gaagctgcta gacaagatca
    27721 agaaacaaaa ttgacggaat gggaagaact aagcttggaa ttagaagaat atgaaaatta
    27781 acgaaaaaac caaaacagaa accattcatg ctgttttggt ttttcttttt ttattttatc
    27841 aaacaaaaat tccatattta cctttttcac caataagaag accaattact agtcattctc
    27901 actaaagctt ctaaataata gtaatcaccc caaatacaag gtgtatttac cccattattt
    27961 ttgggcttat tataaacaga ctgtgttaaa aagccatctt cttcttccgt tgtagcatat
    28021 ctttctttca attgtcgcat aattttccaa acagcagact ggtataagtt cttttctgtt
    28081 ttgtctaata gacctgattc tattaattct aataatccac aaaccatgat caccatacta
    28141 gatgtatctt tttcttctct gctagaatca tcaaagatca aatcccaata acaaatggta
    28201 tcttctggca accgattcat aaaataattg gctaatattt tgcttaaatc aaaatatttt
    28261 tgttctttca aataacgata acttaatgca aaaccgtaaa tcccccatgc ttggcctcta
    28321 gcccaacagg aattatcact ataaccttga tgcgtacttc catatcttgg ttttccatct
    28381 ttgacatcca tataaaatgt atgataagtt gaaccatctt ctcttactaa ataatctgct
    28441 gcattttgaa tatgagcctg agcagctttt ttatattttt gatttccagt ttcttcactc
    28501 gcccaataca ataatggaag attcatgcaa caatccatga tcattctacc ttgttgtgaa
    28561 ggatcactag gatctcccca agcttgtata actcctgcct tctcccaaaa acgctgaagt
    28621 aatgtttctg cagcatcaat tgaagcttcc ttcatttttc gatttcccgt tattttataa
    28681 tctgctacgg ttgataaaga atacaaaaaa ccaagatcgt gatgattagt gataatttga
    28741 cagtcaatcc tgtggataaa atctatatct gctttttcag ctgtttgttg aaataaagga
    28801 tcacccgtct tctcaaataa caaccaatta attcctgtcc aaaatccttc tgtccaacca
    28861 atattgtctc ttaatggata acgtcctttt ataggacact cgcttgggaa ttgatactga
    28921 ctgttactga tacgtttgat taacctctcc gtgtgtctgc gacaacgctc gatctcatta
    28981 ttccattctt tttccattaa ttctttataa cattcattta tccatttttc tggatgtatt
    29041 atctcttcaa acatcacttt atcaagcatt tccccaccag cctttagcat aattttagtc
    29101 tttttttatt gtttttattt tggatagtta gcccggttgt cccatcaaaa tcaattttgg
    29161 gacgattgtt ccatacttct aaactataat cccgatcttg atgagcaacc actcctgttc
    29221 cgagccaatg ggttcctttt ccaaatgtac cctctaatcc aggaataaaa gtgttttccc
    29281 atgtcatcaa gtttgtattg ggcaaaccca taatagctgt ggattttcgt ttaatagtat
    29341 cgtcacttaa acagataata cctgaaaagc cagcttcact caccctatag ttttccttgt
    29401 cttcttctat gattattcct tctttctcag gagaacattc aatagcaaac cctgtctcat
    29461 atagaacata ttctgtatct aattctactt tgtgtacgcg aatctgccag ccttcaaaag
    29521 gaataagcca agttctgatt tttgcctgta aagtaggttc ccatgtagag gcaagtacct
    29581 ctttcttaca tacaatgtct cgtacattat ttcgaacgat gaactgtcca taggttttga
    29641 tgctctcatt cttaaaactg gtcgtgatat atgtgtccgc tcgctgaatc cctagcattg
    29701 aatccattgc catttctgct ttcaattgat tcgctcgcgg tatcgaaaaa ccaaattgac
    29761 tagaataagc aaattttaaa tatttttcat tggtcaatgt atggaaattt ggccctaatt
    29821 gtcccgcatt caaaaaaaat gtatgtccat tatcatgttg tatgagcaaa ttggcttctg
    29881 aaagaagttt agttccttca cgaataggca taggcatttc tttagccgtc caatactcat
    29941 ggtcttcagg tagcgccaac aataaaaaaa tcttattcag ccataatgga gataacgtac
    30001 tattataggg ttcagtcaat gtcaattgag ggtaggtata accaatattc aggatacctt
    30061 tttcatcaaa tatttctttt tgcatccacc atcttatatt ccgattgact aaccccttga
    30121 tttcacctaa tttaaaaggc aaaagatcgg cccacaacat agctgaccaa aagcttgaaa
    30181 ctgcaaaacg ataaatcatg cttcgaccaa aaggcacatt tgcaccatct tccgcaaaaa
    30241 aatgaatata gtctctagaa aaatcaatag ctcggttctt atatatctgg ctaatgggct
    30301 tttctggttc tagtacgcta taaatcaaac cataataatg aaatgcaaaa ggattataat
    30361 aatcttggcg acctcttgaa ctgtctcgat accagccatt ttgaagataa caattttcta
    30421 tattatcaaa tgcttctttc agtgctgatt ctttaactgg ataaccaaat ttttggataa
    30481 ttgttccgac aattatctta aaaaattgcc aattaccatc tgcacattca tattcaaaaa
    30541 tttgcgagaa ccaatgaata atctggcttt tttctcctgt atctaatttt ttccataata
    30601 attgttcatg atgatataac gctaataaca ttgcgggcat ttctaccata cgctggtctc
    30661 tttcatgtat ttctccccaa taatcgggat gggaaggatc tgttccagct tttattcctg
    30721 atagaattaa tttcaaccat cgttcatcta attggtcaat tgcgggcccc gctccccata
    30781 aaacccgaga gaatccttcc attcctgcaa ttttatttcc atatcctacc ccttgatgta
    30841 tataatcaat ttgtgcgcca cttgctcgat aaaacggctg gagtggttga tttatctttt
    30901 taaataagtc aaagtaatcc tgtctggtcc ttaatagtgg tactggatgt ttcattgtat
    30961 tttctcctct ttttactacc aaatagctaa ttccgactgt tctaatcgaa gtaagccttc
    31021 aataaaaaaa taatcagaat agatcagtgg cacattataa gtcagttctc tatggtatgc
    31081 ttctgctccg cctccaatga tcccatcttc atccatattc caatttgcat agtgcttttc
    31141 tgtcttttgg agtatattga tagcagcttc atgatacatt tttgatcgta cagaggaaac
    31201 atatcctgct aattccagaa gaccgcatgc agcgcatact ccagcactgg tatcaatatt
    31261 cgaatctttt acagcaaaat caatgagagg aatataacca gttttttcga ttttctgaat
    31321 aaaatttact gctatctttt ctgctatagc tagatagtac ggattttttg tgtaacgata
    31381 acttaacgcg aatccatata aagcccatgc atttcctctg ctccaacaag aattctcatt
    31441 gtatccttgc ccgccaagta accgaatata tttccctgat tctgcatcaa attctgcgat
    31501 atgcccggtt gatccatctt tcctcactag atacttagct gttgtatctg catgctgccg
    31561 agcaatttta gagaacctcg gatcaccagt ttccctcgaa gcccaataca gtaatggtaa
    31621 attcatcata ctatcaataa tcatccaacc aggtttatgg tcattccatg ctactaaata
    31681 gttccctgaa atattatagc gtccagcaag taccgtagct gcatgtaacc ctagttgtcg
    31741 actatgtttg ttttgagact gttgataatg tgcaatagcc gatggtaaga ataaaaaacc
    31801 tacatcatga tgaacgtctg aaaaatggta cagggcagat tctaataatt gttcttgttg
    31861 tattgctgcc tctttgaact tttctcgttt tgtcttgtga aagagctgcc ataaaatacc
    31921 actccaaaaa ccattggtcc accagtcgat tcctctgtcc tttattacgt ctgtatattt
    31981 tccgttgtaa gaaatatatg gaattttatt ccccattctg tctaattccg catctatctt
    32041 ttcagaagct acattcaaaa tttttcttga ccattccttc gtctgttcat tcatggatat
    32101 cccgccattc ttttaatttt tatgaatgtg gagttgctcc tagaacgaac tagatggtta
    32161 acattcgtta ttttttttac ttatttgcag ctccaatgtc attctcctta aatttagact
    32221 gcgcccacac ataaatcaag accattggga tcactgaagt tgctaaaatt agcataattg
    32281 cgccataatt aattggctca cctgtactag aaaccgtcat atttttgata attaagggaa
    32341 ccgtatattt atctttatct gaaagcatga ctaatggaaa tagatattga ttccaacttc
    32401 ccataaagat aagtaaacca ctagtaataa tcgtaggtga taaaatcggt acagcgattt
    32461 tcgtaaaaat cacccactca ttggcaccat cgattctggc agcctccatc agtgctgtag
    32521 gaaattgact gctatattgt cgcatcataa acactgaagt tgtactggca atcgaaggga
    32581 tgatcacacc tgcataagta ttcgataatc ctaaacgact cataatgtaa aataaaggaa
    32641 tcaatgtggt aaaaaaaggc accatcctag atagcataac tgtattgaac aacagtttat
    32701 tccccataaa acgatacttt gctaatgcat aacccgccat tgtagaactg gctgtagcta
    32761 aaactgtact gattacagca ataacaatcg aattccaaat aatcaaggga taattatatc
    32821 tcgccatgat ggcttgccag ttttttaata gcatatcccc aaacttgaaa ttgattccgc
    32881 cacttaaaat ttcttgatta ctttgcgttg aagctacaat catatataaa tatggcatca
    32941 aaaatattaa ggcaccaaac acaataatga cgtacgtaaa aattctgctt tgtatctttt
    33001 gtttagtcat ttttatcacc tccgaattta aattgaatag ccgataatat aacagataca
    33061 aacaccatcg tccaggcaat agcagaggct tttccaaaat caaccatttc aaaacttgtt
    33121 ttatataaat tgactcctac gaccaaagct gaattttctg gtccattgga agtcccaaag
    33181 aatatatttg ggatattgaa attacccaag ccattgatag tggctaaaac tgtggccatt
    33241 aaaatgattg gctttagcat ggggatcgtt aataaaaaga attgccgaaa tttcgatgct
    33301 ccatctattt cagcactttc gtatatctct ttagaaatcc cttgaagacc agctaaaaat
    33361 aaaatagtgt tatatcctac actaagccaa atcatcgtga tgatcattga tattcgtatc
    33421 caaaaaggat ttgttaacca ctgtacaccg ttaattccta gcgcattcaa aaatccattg
    33481 aataacccag tcggattgaa aaaagtcatg aatacggaag ctacagccac cgttgatgta
    33541 atggttggaa tataatagac agttctaaaa aatccttttc cttttaactg tgctgaattc
    33601 aataaaacag caaagtaaat agctaatagc atcgttattg gaattgatcc tgccatgata
    33661 atcaaaacat tcgtgtaaga tttaataaac aatggatctc ggaagatagc tagataattg
    33721 tcaaatccaa caaattcaaa tgagtagcca attttcataa aactaaaagc aaacgagata
    33781 atgatgggaa tcaaaatcgt cacaataaag aaaataacaa aaggtgtgac caatccataa
    33841 gcactttttt tatgagacgt atctgttgct tttttcttgc tgttctttat tgtcttgctt
    33901 ggtaacgaag attgcatcat cgttaagtcc tcctaatatt ttttaatcac cagttaattt
    33961 tacgccgtac ttatgagcaa aattttctgc ttcttttaga tatgaatttt caaaatcacc
    34021 tgtttgccaa tagttatatg cagcggtagt caaataagac gtagcatctg aataagtggc
    34081 tggtaaaaca gtatttgatg tattttcatt gattttattc aatagttcat agtatttttg
    34141 tcctccttag tagtcaaact ttttctcagc tgcttctgtt tgataggcca atgagttaga
    34201 tgtagccacg cccagctcca gtgcgttggc taagcattct tcatctgtca gcataaatgt
    34261 aagaaattgt tgtgccactt gtggattttt ggcatcttta gggacataaa aacttgatcc
    34321 tcctgatact gcaccttttc ctggattttc ttttgaaaaa ggaatcagtg gagccatgcg
    34381 ccatttgtca gatgcatcag gaaaattcaa agacatattc gtagcatacc agccgccagc
    34441 aacaaacatt gcagattctt gaaatgcttt ctcagcatct tgtgcaccgt agaaatgtac
    34501 aatatctgag tcgatcattt ctttgattaa ctttgcagca tgtttcgctt ctttagtatt
    34561 cagacggata tttccttctt catcaagcaa agattgattc tgctgggcta gcatgttgat
    34621 aaacaattct tggtccccag attctggcaa ggcaattcca aaactaccag ttttttcttt
    34681 taatgctttt gccacctgga tatattcttc ccaagaactg atttcttcaa aatctacacc
    34741 tatttcagca aatttttttg gttgataata acaaagtgct ggggtaaaat cactagccca
    34801 aggaataatc gtttcattac tcgtttgttg acgcaaaata ttcaattttt gatcataaaa
    34861 ctgatcgcca tatttgtcat aaaatccata ttcatcagca ctataaaaag catcttgaaa
    34921 tttttccagc acacctgctg ctgaagtgtc tgccatggag ataatatccg gcatatcttc
    34981 gtgtgttacc aattttgggg tgatcatttg tactgttgct gttcccgaag ctacattcac
    35041 aatttttaag tcaatatttg ctccagtttt gcggttatag atttttgcag cattttcaac
    35101 atagtttgtc agtttgccgt ttgcgacata tacttctaat gtagtggctt tttttatcat
    35161 tggatctgcg atgtttgctg ttacttcttc gttagttttt ccacatgcac ttaagctgaa
    35221 taatagggta atgtttagca aagctatcac caatttgtat ctttttttca tgtctatccc
    35281 ttctttcttt tcaagttctt atataaaacg ctttcactta tgtatattaa cggaattagt
    35341 aacacaaata tttatataaa aaaatatttt acttgtcgtt ttggcttttc atttttgtta
    35401 taatagtcat taattataaa tagaaagctt tttatataaa aagagaaagg aaagtggttc
    35461 ttttgttaac aaacaaccaa tcctccaatc ttgtcaaaaa aacactaacc tattccatag
    35521 atacccatct tcatttatct gctgctccct attttcgact actaataaat gaaagcgcta
    35581 atttatatgc tgaatttgca cataaatcta tagaactaga taatccttcc cttttattca
    35641 ttgataaaca gcaagaagtc caaatctttt ctactcaaaa ggaaacacaa acttttatca
    35701 ttatccagtt ttattttgat gagcaattat tagcacatat tacgggacaa tctttttcta
    35761 tagatcaaga aataacatct atcattactt ttctgaaaaa tgaacagttc aaaattgatt
    35821 atctaaaaca tagtaagcca gctatttcct atgatcagga aatcaattct tttttcatac
    35881 tatgccaaca gatgagcatt catttgcttt ccttattagt tacttattgt ctgcaaaaaa
    35941 ttcaattgtc ttccttacca aggcattatc aacaggaact tgctttattg acaatacctt
    36001 caaatgagaa agtcattcct aaaaaaaagc atattcttta ttcagatgaa ttagttgatc
    36061 caatcatacg atatttagaa gaacatcttt cagaaaattt tttgcttgaa caattagccc
    36121 aaattttttt tatcagtcca gccagcttaa aaaaacattt tagaaaagtg aatggaaaaa
    36181 gtgttattag ctattttaaa gaactaaaaa tggaaaaagc aaaacgtctc gtgctagaaa
    36241 atgaattaac ctatacagaa attgcagata tattaggatt tcattcgatc catcattttt
    36301 cttcagcttt taaaaaatat acagggttat ccccttcaaa gtattatcta tccttaaaat
    36361 agaaaaaaat ttttaggttt tatccacaaa ttatttctct atattttctg tctattcaaa
    36421 ataaacaata attttccggc acaattattt gctgtttttt accataacat agtacgatca
    36481 acgtagaaaa ggagatgttt ttatgaaagt tgttgtcatt ggcggtgtag ctggcggtcc
    36541 ttcatttgcc actcgtttcc gtcgattgaa tgaagcgcac gaaatcatta tctatgaacg
    36601 cggagaaaat atttcttatg caagttgtgc tttgccttat tatttaggag gtgtgatcac
    36661 tgaccgtgac tcgctgatcg aacgtacacc agaaatattg aaaacaaaaa acaacatcga
    36721 tgtctttact aaacacgaag taacagcaat cgatccttct actaagcgat taacagttaa
    36781 agacctatcc acaaatgaag aaataaaaac agattacgat aagttgatca tctcttctgg
    36841 tgctagacca gattatccgg atattcccgg agtttttgaa gcagaaaacg gctttgtcct
    36901 ccgtagtgtg acggatgcgg atcgaatcaa atcgttcctt gaagaaaaaa atccacaaca
    36961 tgtcgtcatt cttggtgcag gtgttatggg tctggaatta gctgagaatt tcaagcatcg
    37021 cggcttagac gtgactttaa tcgatcaatt gccacaagtc gctttccctt atgatccaga
    37081 aattgctaat ttagtttatg acaaattgct gaaagaagga ttagccgttc atttagaaac
    37141 aagagttact gagatccgtg ataaaggtcg agaaatagta ttatcagatg gctccgtcct
    37201 ttctgctgat atgctaattt ttgctgttgg tgtttctccg aagaatgaag tggtgaaagc
    37261 aaccggcata caattatcag atacaggtca gatcattgtt gatgaccagt tacaaaccaa
    37321 tctgccggac atctatgcga ttggcgatat tatcgaaacg actagtgtag tgactggtca
    37381 gccgatccaa agtatgcttt ccagtgcggc caatcgtcaa ggacatatgc tggcagatat
    37441 tttaaatggt acgcctatgc gctatcgcgg atatattggt gcaggtgtcg caaaaatctt
    37501 tgatcataca gcaagttatg cgggaatgac agaacatgaa ttaaaagcat caggcataac
    37561 aaattataaa actgttttta tcactccctt tgaccatgcc tatttctatc cagaagctac
    37621 aagattaaat ctaaagctga tttttgatgc agatagcggt cgtattttag gtggacaagc
    37681 atttggagaa aaaggtgtcg ataaacggat gggagaactt tctgtagcaa tcaccggaaa
    37741 cttgacagtc tttgatttgc ctgatttgga gttgccttac tctccaccgt attctactac
    37801 ccgtgacccg ttgaatatag ctggttatgt cgcaatcaat caaatgacga atatcgtaga
    37861 aacgatcaaa gcaagtgata tacccgaaaa cgatttgaaa gaagcgttct ttttagatat
    37921 acgtgaacct aataaagcac cttctggcag tatctcggca acaaaaaata ttccaatgaa
    37981 tgagttacga gatcgaatca atgaaatccc aaaagataaa aaagtttaca ttactttcag
    38041 aagaggattg aatacttata cttctgcccg aatcttggca ggtttgggta tcaaagcgat
    38101 tttgattgaa gaataaaaaa acgaccgcgg tgggtagccg cggtctagga gttattttta
    38161 cttgaggagt aaaaatgaaa aagtatatag ggttgttggt tggtatgtat atagaataga
    38221 agataaatgt gaagaaattg tgcattattt cttcacattt attaggttat agtttaagaa
    38281 agtttaaaca ttgcgcaatt taattatttt agctgtttct tttcataaaa aaacatgttg
    38341 tgactctgag acaaatcata gtccatagtg atgtctctag agtaactacg cctaatatta
    38401 cgaggcatca tattaatcta tcagcactag tacctaaact agcatccctt tcgaatattt
    38461 tttcttatat aataaagttt gctttcactc ctctaatctc accaaatcga ctggcttgcg
    38521 ttgtgtgata ctaggaactg attggtttag aacgactttt ctattgtaat catatttttc
    38581 gccatcctcg ttgtcttcta atccttcaat gaatgatccc taatgtgtgt attaaataat
    38641 ttaatttttt tgttcctatt tatttaaca
//
LOCUS       DAMEET010000014        37732 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.014, whole genome shotgun sequence.
ACCESSION   DAMEET010000014 DAMEET010000000
VERSION     DAMEET010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 37732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.014"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(159..1469)
                     /locus_tag="PB780_000651"
     CDS             complement(159..1469)
                     /locus_tag="PB780_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HDE0732723.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1481..2899)
                     /locus_tag="PB780_000652"
     CDS             complement(1481..2899)
                     /locus_tag="PB780_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDE0732724.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3139..4707)
                     /locus_tag="PB780_000653"
     CDS             complement(3139..4707)
                     /locus_tag="PB780_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDE0732725.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4711..5121)
                     /locus_tag="PB780_000654"
     CDS             complement(4711..5121)
                     /locus_tag="PB780_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDE0732726.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5118..6041)
                     /locus_tag="PB780_000655"
     CDS             complement(5118..6041)
                     /locus_tag="PB780_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HDE0732727.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6038..6916)
                     /locus_tag="PB780_000656"
     CDS             complement(6038..6916)
                     /locus_tag="PB780_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HDE0732728.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6903..8240)
                     /locus_tag="PB780_000657"
     CDS             complement(6903..8240)
                     /locus_tag="PB780_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HDE0732729.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8243..9346)
                     /locus_tag="PB780_000658"
     CDS             complement(8243..9346)
                     /locus_tag="PB780_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDE0732730.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9420..10928)
                     /locus_tag="PB780_000659"
     CDS             complement(9420..10928)
                     /locus_tag="PB780_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDE0732731.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10894..11586)
                     /locus_tag="PB780_000660"
     CDS             complement(10894..11586)
                     /locus_tag="PB780_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDE0732732.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11608..12582)
                     /locus_tag="PB780_000661"
     CDS             complement(11608..12582)
                     /locus_tag="PB780_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDE0732733.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12882..13754
                     /locus_tag="PB780_000662"
     CDS             12882..13754
                     /locus_tag="PB780_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDE0732734.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14212..16410)
                     /locus_tag="PB780_000663"
     CDS             complement(14212..16410)
                     /locus_tag="PB780_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDE0732735.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16642..17634
                     /locus_tag="PB780_000664"
     CDS             16642..17634
                     /locus_tag="PB780_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDE0732736.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17694..19115
                     /locus_tag="PB780_000665"
     CDS             17694..19115
                     /locus_tag="PB780_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HDE0732737.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19108..20607
                     /locus_tag="PB780_000666"
     CDS             19108..20607
                     /locus_tag="PB780_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HDE0732738.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20646..22040
                     /gene="uxaC"
                     /locus_tag="PB780_000667"
     CDS             20646..22040
                     /gene="uxaC"
                     /locus_tag="PB780_000667"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDE0732739.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22199..22882
                     /locus_tag="PB780_000668"
     CDS             22199..22882
                     /locus_tag="PB780_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HDE0732740.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRPP
                     LANYLQKGV"
     gene            22884..23720
                     /locus_tag="PB780_000669"
     CDS             22884..23720
                     /locus_tag="PB780_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDE0732741.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23731..25485
                     /locus_tag="PB780_000670"
     CDS             23731..25485
                     /locus_tag="PB780_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDE0732742.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25761..26102)
                     /locus_tag="PB780_000671"
     CDS             complement(25761..26102)
                     /locus_tag="PB780_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDE0732743.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26170..28341)
                     /locus_tag="PB780_000672"
     CDS             complement(26170..28341)
                     /locus_tag="PB780_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDE0732744.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28510..29409
                     /locus_tag="PB780_000673"
     CDS             28510..29409
                     /locus_tag="PB780_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDE0732745.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(29480..30337)
                     /locus_tag="PB780_000674"
     CDS             complement(29480..30337)
                     /locus_tag="PB780_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDE0732746.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30322..33024)
                     /locus_tag="PB780_000675"
     CDS             complement(30322..33024)
                     /locus_tag="PB780_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDE0732747.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33037..34326)
                     /locus_tag="PB780_000676"
     CDS             complement(33037..34326)
                     /locus_tag="PB780_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HDE0732748.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34476..35522
                     /locus_tag="PB780_000677"
     CDS             34476..35522
                     /locus_tag="PB780_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDE0732749.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     SYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35524..37677
                     /locus_tag="PB780_000678"
     CDS             35524..37677
                     /locus_tag="PB780_000678"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HDE0732750.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT        11154 a         7776 c         6706 g        12096 t
ORIGIN      
        1 acgacttagt tatcttatca cgtctaaaaa catgtgtcaa ctcttttttg gaattttcat
       61 aaaatgtttc aatgaagcgc gacaacgttg tttaatatat cattaaaaaa aataaaggtc
      121 aagctaaaat attatttaag cttgacctta ttataatttc aacgattgtc aaaagtattg
      181 tttgcagctg tgttgccagt ttctatagat aaatcacagc ttttgaaaca taagttttgt
      241 actgcttcga ttcgcccgaa ttcttttgca gttatttggc aatgagaaaa gcagacatct
      301 tctataggct tttcaggaaa tgctactaaa tcaaaagctc gggaaggaag tgagtaacct
      361 ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt tgactttcgt catttgcttt
      421 gattcaggta tttcttcagc cactgcttgc caactttctg gcatttgagc aaggtcattg
      481 ctattttttc tattgtattc attatgccag tccattatcc aagaaaaagg aaattgtaca
      541 tcaatcatat tcagattttc taccctgata ttttcgatca ctccccctct ttcttttgaa
      601 gatttcattc gaataccaca gtctgactga aaaaaatcga tatcgtgaat atatacgtca
      661 ctaactccag cactaacttc gctaccaata gttacaccgt atcctgaacg gattttacat
      721 ctagcaacct cgattcttga tgattttcga ttgactcttg caccattgcc atctctgcca
      781 gacttaatag caatacaatc atcaccacag gccaaatcac attcatagac acgcacattt
      841 gttgaagaat caatatcaat cccatctgta cttggtccat cattgttgcg gatgattact
      901 ttttctacta atacatcgtt agaataagtg atttgcaagt tccaaaaacc tgatttctga
      961 aaaatcaaat cacgaacaac aacatgttct gcatgataca gtaaacaagc gcgtggacgt
     1021 ttaatagcat aatccgcaat ccaacgaagt cccttttgat cgtattcagc tcttgtcccg
     1081 cttttttgat cttttcccca atacagctcc caccaatggt ctccttgacc gtcgattatt
     1141 ccttttcctt ctataagaat atctttcgcc gagagaacat tcaaaatcgc tgctggccat
     1201 ttcatttcta cgccggctac tcgtgtatcg atttctggaa atgcttcgat atctgttctc
     1261 cccttgagga ctgcaccttc ttcaaagata agatgactct tatcttttaa aaaaagggct
     1321 ccgactaaat aaatgccttt tggaataaat acaggtaagt gttctttcgc accttgatcg
     1381 attgcttttt gcagtattat tgtatcatct tgttgagtgt cgtttgtttt aaagttcaaa
     1441 gcattgataa tccctttttt tgtccgcaat acgtttctcc ctatctttca ttgatttttt
     1501 tcaattcttc accaataaat acagtttttg aacgattcag tgggtaacct actagcaaaa
     1561 tgattgcgga tacaaataag cagaatgctg gtaacaagtt tgccatcgta taaatccttt
     1621 cttgtactaa aactgattgg atggctccac ctgttgtaga cgattggtaa ccaatgagtg
     1681 ccagcataaa tccgcctacc gctcctgcac atgcttgtcc tacttttctc gcaaaagaat
     1741 ttactccata tactgttcca tcttctctta agcctgtctt atattgatga taatcaatca
     1801 cgtcagtaat gaatgcccag accatcaaat tgaataaacc agatcctaaa gtagcgataa
     1861 ataaaagaat cagatatacc catgcatttg ttatatgaag gaaatacatc agcaagtaca
     1921 tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt tttaccaaat aattttgtca
     1981 gctttgtagc aaatggtgct aaaactacta ctgttccgta agtaaataaa agagcaacag
     2041 acattgcttg tttattatgg aagtaatcat tgaataaata agtcatattc gtcccagcta
     2101 aaatctgatt gatcacaatg aaaatgtcaa caatgactaa aataatcagt gctcgattag
     2161 ctaacaaacc ttttaccaac tgagaagcag atactttttc gtttttgaca actcttactc
     2221 tttcagtagt caatttacaa gttaacatat atgcgacaaa tgctattgca gcacatccga
     2281 ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg tccactcttg ttatgggtat
     2341 acattaaaat cggaatgaag aatcctgtag ttgatccgcc aatcgcagaa ccaatgcttc
     2401 ggaacgtgga caacgaggtg cggtcattag gatttgagct aattgctgct gccattgctc
     2461 catacgggat attgattgtt gagagaaaaa tcccataaac aatgtatgaa acaaaaatat
     2521 aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg caaaaacaaa atgacagtca
     2581 ctaagcaaaa tggatacttc atttttctga tccaaggaat aaatcggcca gtttctgtta
     2641 actctgagac atcacataat cttccaacca tcacatcagc aaacgcatcc aaaattctcg
     2701 aaatcaagaa taacgtacct acgattgccc ctgaaacgcc aagaacattc gtataataaa
     2761 tcatcaagaa actattgact aaacctaaaa taaaacagtt acctagatca ccgaacatgt
     2821 agcctatctt atcggaaatc ccaaattcct ttttttcttt ttttattgta tttgctgtaa
     2881 cagcattttg tgaaagcatt tgaattcctc ctacctcttc tagtaaacgt tttcaaaatt
     2941 ataactcttt ttttcttgtt tttatattca caatgttggc tttttattgc attatcttga
     3001 cagataaaaa acaaaaaata atctaaaaac attgatgcat aagattgata acttttttta
     3061 cataacaata tatacaaaca aaaaaattca ctaactagtg aaaataagtt agtgaacctc
     3121 atttattaaa gaattaaatt acagatgatc ttttccatca tacttgaaaa actcaaaatc
     3181 agcataactt cctctttttt tatttagatc atgcactgct atcccgataa aatttccagt
     3241 aaaccctcct gagagtatac acatgttttt ttgcggacct actttcttcc attcttcagt
     3301 tgatacgtct ttcaaatgaa actgtgctgt ctctttcttt acagtgactt tcaaaaaagt
     3361 cttcttttct gtgagtggaa tcgtccaatc ttctatttgt gctactccgt tttctgattc
     3421 tatcatacgt aggcattttc ccttgtcctc attccaagta acataacaat aaatataatt
     3481 ggattcattt aagtacagta ccagtccagc catctgatta aaattattag gcatgtattc
     3541 aaatgctgtc tccgcatcaa aagtaaaatc cgtttgccga atagctaaca aatgatgttc
     3601 gaaggtagat tgaagagatt caccggaaat cagacgtaaa aaacctggtc tacattttgt
     3661 atcacaccaa tcagcatttg gcaaaatgcg tctggcattc cagttcttct ttagctctcc
     3721 ttcaaatgta tccataaact gatgatcttt ttcttgttgt acctcttcat ttgtctcaat
     3781 cattatttct tccatcggag ttgtctggct atctttaagt cttaaccaat cgtcggacgt
     3841 ccaatacact tcttgaatag cggtttctct tcccaaaata gccgcaccgt ttaatggtct
     3901 ggtacaaaga tatgtcatgt accatcttcc tgacggggtt tgtactaagc tcccatgtcc
     3961 tgtacattgc aatgtagaag caggtttgtc actagctgtc agcataggaa aaccaggatc
     4021 aagctcgtaa ggaccagtaa tattctttgc tcggcaaact gtaactgaat gtcctcttcc
     4081 tgtaccgcct tctgccgtca tcaaataata atacccgtta cgaaaataca aatgtggtgc
     4141 ctccgttttc gctaattctg tacctgcaaa tattttatag atgggaccat caagcttctt
     4201 cttctctgca tcatattctt gtaatacgat accgctggat ttattcggtg tgtccagacg
     4261 ataatcccat agttcattga caagccattt ttttcccgtt tcaagatcat ggaacaaaga
     4321 aggatcaaag ccgttactat ttaaataaat tggttcggac caaggacctg tgatctcaga
     4381 tgcagtgatc aaataattgt ggacatcttt aaacggacgg cctgcacttt tgacatctgt
     4441 gtaaacaaga taaaacaatc catcagcata actgatctgt ggcgcccata tacttccatt
     4501 tgtaggattt ccttgcaaag atacttgagt ggtcaaaata tccgttgtat gtgaccagtt
     4561 gactaaattt ttcgactcat atactcggat tcctggaagc cattcaaaag aggagacaat
     4621 aatgtaatac cgctctttca ctcttaatat attaggatct ggattgaatc ctcttaaaat
     4681 aggatttttc actttgatag tctgcatgat tcaggctcct tttctcattc ttactcgata
     4741 taaactgaat gccccagctg cgaaataaaa aaatgcaaaa ggaaacgcaa taaactgagt
     4801 gccaatcaac aatatgaatc ctgttataaa atacaaacca gtggcagttg ttaagtattt
     4861 tcctttagca gtcaaaaaca atgctactgc taaaaaagca agtgaaataa acaacacaac
     4921 ggcaaacaga ccagtcatgt cctgtaataa tccaaatgta ttttctaaag acaaagttga
     4981 atgatctaca gcaagcgact gataactttt tttaaactct gacgcagaca tattacttaa
     5041 agtaactgct aacccgccaa agaaaatcaa agatagactg ccaccgacca ttcctagcaa
     5101 tagttcccat tttcttttca tttctgaacc ccctgctttt ctaaatgtat ccattcaggt
     5161 cttttgatcg atgtttcata attgttatta cgttcagaga atttgacagt ctgttcattt
     5221 ttcttgtcat cccaattatc ccaaccagtt gggtgaacat gcccacctat cttacacgct
     5281 tcaaataaga catttgcata cggacgccac ggtcttccta aatagacgtt tctagtatca
     5341 ggttctgcag tcaaggtaca gctttcaaaa cgataccctg ttttttgatt tttcggcgta
     5401 gatgctgctg tgatgtatcc aggattttta ctggctaagc tatggatatg acactgatga
     5461 aaaacggcat ctgccccacc aaatatgaaa tccaccgttc cttcaatcca gcaatattca
     5521 taggtctgac gacaatactc aggattggca gaagccggtg tacaaaaagg agtaccatct
     5581 ttttgcaaat caggcaacgg acccgtgcat aaggtatcct gataaccaga tagtcgacag
     5641 ttttttaaag atacgcgatc tcctgaattg aataaagcaa cagcctgtcc taccttttct
     5701 cctggacctg ctgtattttc gatgtgaaga ttttctaata cgacattcga tccttctaag
     5761 aaaaaagtag cacttcggaa agtccctaac tggctaccgt caagatactc ctgttttgca
     5821 aaacgattac cggagataac tactgttcct gctcccacaa gtttaaaatt ggataatctg
     5881 ctttctatat attcatcata tcttccttct tttactatca agacctttgg atggctatcc
     5941 ggcaggcctg ccaaataatc gattccttct tggatagttt caaaatccgt cggggtatct
     6001 atagcattct ttccaattat cagtttgtct ttcatcctca taaatgctcc cttatgaatt
     6061 gtttccaaat atttctcatt tcttgtgttt ccatatgacc gcctgttaaa gaaaaagaag
     6121 aaaagttttc tggactaccc attgattgat actttttgtc cagccctttt ctcagcttca
     6181 gtacgccttc gtccaagcac atcgtatcgt tttttccaac taagctaaga cgagggcgcg
     6241 gagcgatttt ttcttggatt gctaacgttg tataagctgt caatagattt ggcacatagt
     6301 aataaaaccc atgatgatct aggcggcgat gtagcaaaag tatttcgatg tccacctgtg
     6361 ccgctagatc aatacaaact ttgacacgag aatccaatgc tgtcacccac cagcttaata
     6421 agcctcccat agaaagacca atcgttgcga gccgttgagt atctgtgtct tcccttgtct
     6481 ccaaataatc gatcaagctg atcacatcat aaatacgcat tccccataac gtttttcctg
     6541 ttaataaaaa ttgtttgaac aactcgcttt cagaaatacc tccgcgttct tcaaatcccc
     6601 aagcatcaat cgaccaaaca gcatatccca ttcctgtcag tacttctgca aaagatggag
     6661 aagctaagta aggagcacca tggatcaatt cacttttccc gactgaaaaa tctccgccgt
     6721 gagaatgcag gtaaaggacc ggcggaaacg gtccttcttt ttctggataa gcatagaggg
     6781 ctggtactgt ttccaattca tttaagtaca attgatagta ttcaagcata aaaccgtttt
     6841 gtttttctcg gtataatcgt tttccactag gggtatgtct gctagatgga gcacctaaaa
     6901 aatcattcaa ctgcataagc aaagttttta cgaacaacca gttcatccat attttctgat
     6961 tgatttgatt ggaatttctc aatactgatt tctttagtat tttctaatag aaatgctggt
     7021 ccctcgtgat tttcaatcga catctggtta aaggaaacct tacccgcaaa gccaatataa
     7081 aatccttgtc tgctcatttc ctctattcct gtcatcatag caggaacacc gggaactgca
     7141 tcttgggcca tcgagacatc gatattgtca aatgtgattt cttgtatgaa ttgttccgct
     7201 aatccataaa taaaacctgc agaagcatgg acattccttg ccgtgatatt cgaaaaatgg
     7261 atccttctga atgctggtgt ccgttcatct atcggataag cctttttttc ccatacatac
     7321 ggttctttcc ctcgcggacc acagaaataa taaagattca gtataaatgg acacatcaca
     7381 ttatccatga ctatattgga tactcgaata tcttcaacga tcccgcctcg acctcgtcta
     7441 gatttcaatc gtattcctcg atcagtttct tggaagatac aatttgaaat ggtaatatta
     7501 cgaatactac cactcatttc acttcctaat acgacaccgc catgtccatg gaccatcgtg
     7561 caatttgtga tcgtgatatt ttcgcaagcg atcctttcgt acgtatcttc agtccctgcc
     7621 ttgattgcaa tacagtcatc tcccacgtca atatggcaat tactaatacg cacatttttg
     7681 catgattctg gatcaattcc gtctgtattt ggtgaatccg ctgggttcaa gatcgtcaag
     7741 ttatcaaacg ttgcattact gcaaagaatc gggttgatcg tccaacttgg tgactggatc
     7801 aatttaatat ctttcacagt aattcgatga cagttatgga aactcatcaa tttaggacga
     7861 ggataagcga gattatctgg ttcatttcta aaagtatgcc accatttttt tccatttcca
     7921 tctaatgtac cgaaaccagt gacagagatg ttctctacat tttgagcata gatacaggaa
     7981 gcataaactt ttcgatggac tccttcccaa cgtgaatgaa caacaggata atcttttgga
     8041 tcgtcagaga atttcagaac cgctcctgcg cttaaatgaa gttcgacatt gctttttaaa
     8101 aataaagctc ctgttagaaa ttcacctgct ggaacaacta cggtcccgcc tccgtcactt
     8161 gctgcagcat caatggcttg ttgtattgct tcagtattca attcatcaat tgatgcgcca
     8221 aatgtcaaaa tatcatacat atttataatg cctccacttc aatcaatgct tgcaagaatg
     8281 caccaacgcc tttttgatca ttacaaataa ttggttcact gatgtagtaa gcatatgaac
     8341 catctcgctg atctgctccg cctaaccctg ctacttgaca gtttttattc aagttcagcc
     8401 agccttcttt tgtttccaga acaaattcat caagtaatcc ttgatgtgat ttcttcactt
     8461 gtggaagcca ttcttctttg ttcagcagtc ccaaacgtat tcctttagcc attgcgtaaa
     8521 caaacatact actgccggaa gcttccagat aatttccttt ccgttttgtt tctgttgtta
     8581 cttgatacca tgtcccgctg ttttcttctt gatatggaag taatgcagcc agtgtctttt
     8641 ctagaatttc tttcagcaaa gaacaatctg tttcttcatg aagtaattca taggtatctg
     8701 ctgcagccat caaataccag ccaatcgatc ggctccaaaa atgatgggat agacctgttt
     8761 ttggatcagc ccatggttgg accttctttt catcccatgc atgaatcagc aaatttgttt
     8821 ttggatcgaa tagatgttta taactgattt caaactgtct caaaacatct tggtaatctt
     8881 tttcttcact aaaattctgg ataaattcag cataaaaagg agaacccata tataagccat
     8941 ccaaccaaat ttgataagga taaatttctt tatgccaaaa agctccttcg gatgtgcgtg
     9001 gatggtttgc caactgctgg gaaaggagtt ctgctgcttt tttatatttt tcttcgcctg
     9061 tttctttgta caataaaaag aaaagctttc ctgtatttac atggtcgata ttgtattctt
     9121 caacgctgta gccacggatc gttccatctt cttgcacgaa atagtcgata ttgtctttta
     9181 tgaaatcaaa ataaattttt tcttgggttc gttcccaaag taattggaac cctttaagta
     9241 cgacgccata gtcataagac cattttcctt tataccactt ttcttcatat aagcgaggtg
     9301 ttctttccat gatacttttt gctaatttct ttgaccagtt ttccattatt tgctctcctt
     9361 taaaaattaa aataggattt gggacaaata ccaagaagta tttgtcccaa tcgtctatat
     9421 tactgtgccg ctgctttgta tgcttcctcg tactcttgtg tgattcgatc gcctccttgt
     9481 gctttccagt cttctacagc ttttttgaat ccatcttcgt caagctgtcc catgatgtat
     9541 tgcaccgtag catcaatgac gattttttca atttctgatc ctacttcatt gttcgtcggt
     9601 gaatccaatg gcacagaagg atccattaca gcaaattctt cattttcttt gatcaattga
     9661 ttagagaatt ctttttcagg gtctgtatct ttgaaagaga acgtcacttc actaggacga
     9721 gagcttgata acggttgcac ctcttgttgc caaagatcca tatcgataaa ttcgatagaa
     9781 ccatcatcat tcaattcata atgcgttcct tcgataccgc cagtcatcaa tttatacaca
     9841 tcttcttcta aaagatcatc aacaaattga agtaatttct tcaattgtgc ttcgtctttt
     9901 acttgagatt tgggaaatgc tagcaagccg ccaataccat ttccttctga ccaaacatga
     9961 taaccgccgc cttcaccatc tgagatcttg tttacaggca ctaattccat atcatcttga
    10021 ataccttgac ccatcgtctt cagattcttg atatccacca ttccagtgta gattccagtt
    10081 cttccttgtg caaattgttc ttgctgatca gtcttttggg taactgcaaa gtcttgtgcc
    10141 aaatagccat tctcaaatag atctcttgaa aaattcaaag ctttcatata ctcttctgta
    10201 tcaaattctg gagtgaattt gccattatcg tctacagccc agccatttgg tgctccaaaa
    10261 taagaagtga aaagtttaaa gctggtataa cgaatatcac taggcgcacg atcgccaaaa
    10321 ccaactgtgt cgtccacacc atttccatca ggatcgtttt ctgtaaattg tttagctact
    10381 tcataaagct catccatggt agttggaaca tctagtccta gattatccaa ccagtctttt
    10441 cgaatgacta agccagcacg agcgtagtct ttttgtatcg gtacgccgta caaggcaccg
    10501 tcgatactcg cagcttcgat tctatcttca gaaatttgac ttaaattttt atagtctttt
    10561 aaatattctg atacatccca gaatacgcct gattttaacg cattgcggac agaagagttt
    10621 ttcaacatcg caatttgaag tgtaacgata tctcctagtt catcagaagc caatgcagtt
    10681 gttaatcttt cttcttttga agcatcagga atccagttga atgtcagttc cgtatcggtt
    10741 ttttcttcga tcaagttcac aatcgtgtct gtaggcggtg aagcatgatg caaaatattt
    10801 aaccaagtga ttttagcttt tccatcttct gtctctgaag aactgttact tgaactgctg
    10861 ccgcatgcgg caagtgctag aattcctgca aaactaagac ctaattttac gagcgttgct
    10921 tttttcataa attttctcct tcactgtttt aataatattt ttgttttctt ctgttttttc
    10981 tgtatatgag ttcttccatt ccaaatggca aaatatagaa tttgctgtcc acgtgactgc
    11041 ggaaaagaag agactgccac cgataaaggt taaaacacct ggcattgttt ggatgatcat
    11101 cagataaatc cctaccatac ttaatatcaa cactaatgag taatgaagat gtgagaatcc
    11161 gaataaaaat gccattttca ttttgaaaaa atatcctttc caatcataat gagccatgat
    11221 tgggaaaata tagagtgctg agagcacata tatgaacgca gcaatataca aagcgatcct
    11281 aagataccaa ttgtgtatga aagaaagatc cacaagtaaa acaatcccga tggcgccata
    11341 tatccagctc atcagcaagg tttcacgata gttttcttta aaatatgtga agtacgtttt
    11401 cgtcaaagga aacgtttctt ttgagcgcag ccattttcta atcactgcaa ccatcgcata
    11461 cgatgcagga ccgaaagtaa aaactcccaa acctgcgatg actaaaccgc cccacagaaa
    11521 attcaaataa accaactgta aaatacgatt tagccttccg tttattttat ctaatgactg
    11581 cagcatatct ttccctctct ttctatctta gtaaacgccg tcttctccta gctttttggc
    11641 aatcttatta gctaaaatta ccataaacaa tcctacgacc cctttaaaca atcccattgc
    11701 tgtactgaaa cttaactgtc cattcttcaa tccagctgta taaatatagg tgtcgaaaat
    11761 ttctgcaacg cttcgattca atgaattcaa taacaagtac atatgctcga accctaagtc
    11821 taatgtctga ccgattttta gaatcagcaa ggtaatgatt actggacgaa ttgccggcaa
    11881 tgtaacatgc catgttttgc gtaagcgtcc tgctccatcc atttctgcag cttcatacaa
    11941 ctgtgtatcg actgcagtta tcgctgaaag gtagatgatc gtagaccagc ccaactcttt
    12001 ccagatcact tgtccgatat atactgtgcg tgtccattca ggggatgtta gaaaactgat
    12061 tttttcaaat cccattgttg caaggaattc attcaatacc cctccgtcaa cattcaaaaa
    12121 tacataagta atcgaaacaa tgattaccca agacatgaag tgagggatgt aaatgatcgt
    12181 ttgaatcccg tttttcaatc gtttgttcgt tacttcattt aatagtaaag acaaaatgat
    12241 tggcatcgga aaaaacacga agatatttaa tccgaacaag atcaacgtat ttcttaataa
    12301 tgtaaagaat gtcggttctg taaacaaacg aatgaaatgt ttgaaaccga cccaaggact
    12361 tcccattatt cctaaaaatg gttgataatc ttgaaaagca ataacgagtc cccccatagg
    12421 aagataccta aaaatcaaga aataaaggac tcccggcaga atcatcacat ataaaaattt
    12481 attcgttttg attcctgcaa ttattttttt tcgtcttctg agcttttctt cttttttcga
    12541 tagcgtcagt tgaaatggtt cttgtttctt tacactttcc ataagcccct ccttagtttt
    12601 gtgaatgttt gctgcttatg acttcatgat agataactat ctgaattacg tatataatca
    12661 tttttaccgt aagtaaaaac gcttacatag caatgatttt tgtgctatac aaaacgataa
    12721 agtgtatttt ttcacaaata aactacgcat agcaaacctt ctattttttt ctatctgaca
    12781 cttagcatag gaaatgatta tatacgtaat ctttcaccga tgataaattg aacatataga
    12841 aacagaaaca tttaatttat tttgaggagg agcctatcta tatgaaaaag aaagtctcta
    12901 taggagaaag aagttttaca cttttcaaca atacgtttct aattttgcta gctttgatct
    12961 gtatcgttcc ttttttaaat atcatcgcta catcttttgc ttctacacaa gaagtcgtcg
    13021 cgaaaaagtt tatccttttt cctactacct tttctttaga cgcgtatcgc tacattcttt
    13081 ctacgccgac catttttaga gcacttgctg tttcgattgg tgttacaggt gtcggaacga
    13141 tcgtcagtat gtgtgcaact tcactgatgg cctacggtct ttccagaaag tatttgtttg
    13201 gtcgaggttt cgtcaacttt cttgttgtat tttctatgtt gttcagcggg gggatgattc
    13261 caacgttctt agttgttcgt tctctagggc tggtcaattc ttactgggcc atgatcctgc
    13321 ctgtggctgt taatgcaatg aatatgatca ttatgagaaa ctttttccaa gccttgccgg
    13381 atagtttgga agaatcagca aaaatggatg gttgcacaga ttttggtgta tttttcaaaa
    13441 ttatgctgcc attagcgtta ccgtctattg ctactatctc gcttttttat gctgttactt
    13501 actggaacac ttatatgact gctattttgt atatcaacga ttcatctaaa tggccgatac
    13561 agattctttt acgccaaatc gtcattgtat caagtgggat gcaagcagaa agtagtgccg
    13621 ttgatgtgat tccaccagca caaacagtta aaatggctgt tatcgttatt gcaacagtgc
    13681 ctatgctgat tgtttatcct tttgttcaga aatattttgt caaaggagca ctcgtaggtt
    13741 ctgtcaaagg gtgattatgt actgaacgca aaatggtagt tgtgtcagaa gtggtcagct
    13801 tcaagaaata agaaggcatt tacgaaaatt gcttcttaaa tttttgtgaa atttcggctt
    13861 atttttcgaa gaatctactc ctggaacacc gtgtatcaga ggttgtgaaa gagacgttca
    13921 gccttgagcg atgataaaaa ctctcacaaa cattgcgacg agtaacccac aggagcaata
    13981 agaagcgatt ttgaaaaata gcttcccata tttcacaaaa ttatgcctta ttgtcgatag
    14041 cctgtctctt gaacgccatt tattcgtaaa aaagaactgt gacaagactt ttgtcacagt
    14101 tctttttgcg tgcttagcct tgaaatttaa ctatccataa atacaaatac tagggttatt
    14161 ctaacttctt ttattgcttg aatcagtcca caagaaaaac agcctcttga ttcacaaata
    14221 tttttttcga tattctcccg gcgtaatcga ttccttcttt ttaaagaaac gaataaaatt
    14281 ttgcggattt cgatattgca atctttctgc aatctctttt acggagaggt tagtctcttt
    14341 caaccatttt tttgctacct ctaaacgatg attttgaaca aaatcaccaa atttttctcc
    14401 tgtttccttt ttaaatacat tacttaaata gttagggtta taatgcaacc tatcagcgat
    14461 cacatctaaa gacagatcac gatcatattc tgtgtagacg atatgcataa tattctccga
    14521 caaattttta aattcatgat cagtgattgc ttgagagcat tctgtgattg gcagaactaa
    14581 ttgttctaaa acaagttttt ctagttcttt gggatgataa ttgttcagtg cttttacata
    14641 aatttgtttc atgttatcaa agttttttga atctactccc aataactgtc ctaattgaac
    14701 gagttcattg atcaagcgga ccgaaactac ttcccgactc aaaggatttt tattttgtgc
    14761 aaacaatgca tcaatcaatt catgtactaa attcttcact tcctgatttc cagaacgaat
    14821 ggcttcaaac aatcgatttt gttcttctat cggatatcta ataagtgttt tttcatgagc
    14881 agcaggaacg atttcctgat aaaaaataat tgaacttgga ccagtattta cacgataata
    14941 cagggcttct tttgcccgat caaccgattg cttgctttca ttcagcgttt cgaaccggtt
    15001 actgatgcct atactgatag ttaaattcaa gtattctttt acacgttttt gaatgagtcg
    15061 gcaatactcc atgacttttt tgcttgcttc tgcatcatct ttatccattc gataaatagt
    15121 ggcttgcatt tcttcattca aaacgatcgg cagcattcga ttttcctttg gcacaatttc
    15181 cgtgatcatg ttgttgatcg ctaaaagtag caaatcacgc tccccaccgt gactatcttc
    15241 taacagatcg atttgaacca aagctgtata atagcagtca ttttgagaac tataaccaaa
    15301 ttgctgcaac cgctgatcta gttctcgcct ttctactcga tttctaaata aactaagtac
    15361 aaatagcgtt tccagctgtg gcttttgggt aactaagtgt gcacttaacg cttctttttc
    15421 tcctatgatc cgactgactg attgagcgac caaatctagt tctttcccac tgaatccttt
    15481 tgatttaaga ttcagacgtt cctgtatttg agaaatcgga cgggcaaatc tgtccgaaaa
    15541 taagtaggaa agaagaccaa tgagtcctac cagtgttaaa gacactgcaa ctagcccgat
    15601 tctcagattg cggatcattg cgccaattgc actagagtca acttctaatc tgtaaatcca
    15661 attattataa tctgatttca gtactatata cgttttatct ttttctctta tcactttcag
    15721 ctgattgtaa tctagatcca cgtccgttaa tacagttggt tgctctttat tttttactgt
    15781 ctgagtagta aaaaggttgc caacttcgtt ttcaatagaa agtatgtctt ctccactttc
    15841 accaacaact tgttctattg tatgtttctc tatcgtagca attcccaatg catatttttc
    15901 tttttcaaac attggcaaag aaatcaccat ttcgattcct ttgtttttct tttgccaaaa
    15961 taaattattt ttgttattta tgtacttctg ttgatacttt tcaatttcct cttcggtcag
    16021 ttgactcaac gatccattga ttactcccca tttaccagcc aaactgacta aatcataatt
    16081 attatcatcc ataacaatcg tttctataaa atttagttct ttcctgattt cagaataagt
    16141 ggagtaatct gtatatgtca gcggatgctt gaatatgtcc gagaaatttt tggtcgaacc
    16201 atacgtagaa aacgaatatt caatcgttct aattttccat tcaacgttag ttttaacttg
    16261 cctgagcaaa ttctttttgt ctgttaaata actcgattcg atcgattcat aggtattctg
    16321 gtaaatgaat aaactaaatc ctatgacaag tacggctcct aataaaaaca tcggtaaaaa
    16381 tattttataa aatactgtac ttttcttcat tttcttcctc tcctcctttt aaaataaaag
    16441 gctttcattt ttctgtaaaa ttgatactca tctaattaaa tgagcattcc ccgctttcaa
    16501 gaatagcata attaaaaata aaaatccaag taatttttaa cgtgtgcaat cttttcatat
    16561 caaagatcta ttgacttatc gtagttatcg taatatgatg aatgcgttaa caaaaagtga
    16621 aaaagtaagg aaaatgatgg aatggtaacg atcaaagatg tcgctaaagc agctggcgtt
    16681 tctcatacga cagtttctcg tgctttgaat aacagcagtt taataaaaaa tgaaacaaaa
    16741 caaaaaatac gacaactagc taaggaaatg aattattcgc caaactatag tgcaaaaggt
    16801 ttagtgaatc aaaaaaaata tctaatcgga ctatttttct caagtttgca taaaggaact
    16861 tcatcaaatt tcctagcaga ttccattgca ggcatccatt cagtattaga cacaaattac
    16921 agtttatctg ttgaaagtat cgatgttgtt gatttaacag aaatcaacct gcaacgctac
    16981 gatggactga tcgttatgag tcaaagtgat gatgatcagc ctttcatcga ctatctcaaa
    17041 gaaaaagaat ttcctttcgt tgttgtcaat cgtcacatca atgatccagc ggttatcaac
    17101 gtcactgccg atgatgcggc cggtgttatg caggcagtag attacggtat tggatgcgga
    17161 catacgaaga tcgcttatat tggcggaaag aaaaattttc gttcgacaaa agaacgggaa
    17221 aaaggggtat tgaaaagtct aaaaaaagcg agcatccctg tcaatccagc ctatttttta
    17281 acaggagatt acagcttaga aagcggcctc caaaacatgg aatacctatt atcactttct
    17341 gacctgccta ctctggtttt ttgcggcaac gacgacatag cgattggtgc attgagagct
    17401 gtacatttag ctggcctccg tgttccccag aacatttctc tgatcgggtt cgatgattct
    17461 ccagtcgtct cctatctcaa tccgccgctt accaccgtac gtaagccttt gaaagaaatg
    17521 tgtcaagaag gaacaaagct tttactagat ttgatcgaag gcaggacgat cgaacaatct
    17581 aaaattgagc tctccacctt gttgatcctt cgtgagtcga tcaattactt gtaaactaca
    17641 agtaatttta tataaaaatg ttaacgtgtg cataacacag aaaggaagtt tatatgaaaa
    17701 aactgacaaa agaattagtg aaaaaaaacg aagcacctgt aaaagttctt cagtttggtg
    17761 aaggcaattt tatgcgtgga tttattgatt ggcagattca gcagctgaat aaccaacaat
    17821 tgtttaaagg taatgttgca atcattcaac ctttagaaca aggtcttgga aatatgatga
    17881 accagcaaga taatttatat acggtcattt tacaaggact cagcaatggc gaaatcgttg
    17941 atacttccga gattatcacc gtagtagaac aaatgatcaa tccttatgat gaatgggatg
    18001 cttatctatc tctagcagaa aatgacgact tggcctatat cgtctccaat acaacagagg
    18061 caggaatcca attcgtggag actgataatt taacagatac tcccccacag agcctcccgg
    18121 gcaaactgac agccttttta taccgacgtt tcaaattaaa caaagctggc tttaccatca
    18181 ttccttgcga actgattgac agaaacggcg aacgtctaaa agaaatcatt ttacgctacg
    18241 cagaaaaatg ggatttagaa cctgcttttg ttgagtggtt agaaaaagaa aatgttttct
    18301 gttgtagttt agttgatcgc atcgtcccag gttatcctcg cgaagatgca ggtgttttca
    18361 atcaacggca taactatgaa gatcaattga tggtcaaagc agaaccattc atgctttggg
    18421 tgatcgaagg gcctaaagaa ttggagaatc aactaccatt caaacaggct ggcttgaatg
    18481 tcatcatcac agatgatatg actccttatc gtgaacgtaa ggtgcatttg cttaatggac
    18541 cgcatacagc gatggtccct cttgctttgt tggcggaatt ggaaactgta gaagaagtaa
    18601 tgaaagatcc tttattctcc gcatatgttg atcagttatt caatctagaa ttgattccta
    18661 tgctttcttt acctaaagat gaattagcca tttatgctga ccaaatcaaa gaacgtttct
    18721 tgaatccttt tgcccatcac aaattagaag cgatttcatt aaatagtgtt tcaaagttca
    18781 gcacccgtct gcttccagtg ttcaagaagt atatcgaaga acaaaatcag gtaccaccgt
    18841 taatcactgt ttcactagct gcgctacttc ttatgtatcg aggcgatcaa gtaaagccac
    18901 atgatgatga aaagacgata agcgaattta ctgatgcttg gtctgataat ggaacagcta
    18961 ttcctcgatt attgcaaaac gctgcgcttt ggggagaaga tttgtctcaa ataccgaatg
    19021 tgacagatac agttcaagaa atagctgcaa agatcgatca aaaaggtgtc cggtcagtat
    19081 tacaagaaat gaaaggaacg atggtccatg cttgaaaccc tgcaaccaac aatgaaatta
    19141 aatcgacgag acagtgtggt cgtcgcatta acccctattc caaaacaaac acaactgacg
    19201 attgatcagc aaacgatcac cactcttgaa gatatcccgc aaggtcataa gattgcattg
    19261 ggtgatttga aagctggaga caacgtgatc aaatatggct atccaatcgg tcatgtgacg
    19321 acagacgtaa ctgccggtca atggctgcac actcataatg taaaaaccaa tctttctggt
    19381 gaattggatt atcgatatga aaaagatgtc catccaagtc attacctttt tgagaaccga
    19441 acatttcaag gttacttgcg aaaaaatgga aaagtaggga ttcgtaatga tttgttttta
    19501 gtacctaccg ttgggtgtgt gaatggcatt gctgaactga tcgtcaaaca gttcaaggaa
    19561 aaacatccag atctgggaca gtttgaccat attacgattt taaagcatcc ttatggctgt
    19621 tcgcagcttg gaaaagatca tcaaaatact cgagaaattt tagcggacgc agtaaatcat
    19681 cccaatgccg gaggagtttt agtatttgga ttaggttgtg agaacaatac agttcctgaa
    19741 ttcaaaaaaa ttttaggtga atatgatgaa gaacgagtga aatttctagt ggctcaagat
    19801 gtttacgatg agatcgaaca aggtgtggct ctattagaag agcttttaac agcggcggaa
    19861 aatgaccagc gcatatcagt cccgctctct aagttgaatg tcggtttgaa atgcggcggt
    19921 tctgatggtc tatcgggaat aactgctaat cctttacttg gtgcattttc tgactacttg
    19981 attgctcaag gaggcagcac gatcttaaca gaagtgccag aaatgtttgg cgcagaacaa
    20041 gtattgatgg cacgagcgga aaatgaagaa gtttttgact caatcgttga cttgatcaat
    20101 gacttcaaac agtatttctt gtcttacggg gaaccagttt acgaaaaccc atctcctggt
    20161 aataaagcag gtgggatcac tacattagaa gataagtctt taggctgcac gcaaaaatca
    20221 ggaagctctc ctgtcgttga tgtgctacaa tatggcgaaa aaatcagaaa accagggctt
    20281 agtttattgc aagctcccgg gaatgatctt gtagctgcct ctgctcttgc ttcatccgat
    20341 tgccagttgg tcttgtttac cacaggaaga gggactccgt ttggctcata tgtcccaacg
    20401 gtcaaggtat ctacgaatac aactcttttt gaacgcaaag gtcattggat ggattttaat
    20461 gcaggcgtct tattagaaca accaatggaa gtaatcttag aatcgtttgt tcaaaaaatc
    20521 atcgctgtag ctagtggaga agaaacgaaa aatgaacaaa atgacgttcg agaaattgcc
    20581 atttttaaaa acggagtaac actttgattt tcataaaaca gtataaaaag aaagaaggaa
    20641 gatttatgtt tttatctgat gattttttat taaaagacga ttgggcgaaa aagctttacc
    20701 attcctacgc aaaaaacatg cccattattg attatcactg tcacttagat ccaaaagaaa
    20761 tttacgaaaa caacaatttc agcaatctga cagaagcttg gttaggaggc gatcattaca
    20821 aatggcgctt gatgcgagct tgcggcgtgc ctgaagaaaa aatcacagga aatgcttctg
    20881 attttgaaaa atttttagct tggtgtcaaa ccgtaccaaa aattatcgga aatccgcttt
    20941 acagttggtc acatcttgaa ttgaagcgtt tcttcggtat tgacttgttg ataaatgaag
    21001 aaaatgcaga gaaaatttgg acacaagcaa atgaaatttt ggcagctcct gattttcgcc
    21061 gtcgggaaat cgcaaaaaaa tcgaatgtag aagtgatttg tacaacagat gatcccgcag
    21121 acgacttgca ctatcatcag ttgcttgctc atgaagaacc agatttgaag gttctacctt
    21181 cattccgtcc agataaagca ctcaatattg aaaaagaagg atttaatgca tgggtcacac
    21241 tactggaaga agcagctgac caaacgatca ccacctatca agaactgata gaagtcttag
    21301 ggcagcggat agaatacttc catcaacatg gatgtcgtgt ttccgaccac gctttagaca
    21361 tcttacgcta taaagaagcg gatgaaacag tattagaaga gatttttcaa aaagcacgag
    21421 ctaacaaact attatcttca gatgaaatcg atgcttatcg tacagaaaca ctgatacatt
    21481 tgacccactt ctatcattct cataattgga cgatgcagct gcacatccat gcttatcgta
    21541 actgtaatac acaaatgttg gaaaaattag ggcccgatac cggctatgat ggaatgaatg
    21601 atctttcttt gactattcct ctgcaaaaat tattgaaccg tgcagaagaa acggatcagt
    21661 tgccaaagac gatcctttat tcattgaatc cgaatgacta tcctgcgatt ttagctttga
    21721 tgggctgttt ccaaaaagaa cagaatggaa aactgcagtt aggttctgga tggtggtaca
    21781 acgatactcg agctggtatg cgcgatcaat tgacacaatt tgctgatggt agtgctttgg
    21841 ggaattttgt cggcatgctg acagattcaa gaagtttcct gtcctacact agacatgaat
    21901 atttccgtcg tgttctttgt gaatttttag gcgaaatggt catgcgagga gaagcacctg
    21961 aagatgaaca acttttagga agtctagttc aagcgattag ttatacaaat gctaaaaatt
    22021 actttggctt ttttgcctaa ttgattaata gtaacagtaa cgaatcaaaa acaggatagt
    22081 gaaataacgg aatccgttta tccacgtttt agagaagaag acggaccaaa tttgctccgt
    22141 cttcttctct tgctgttctg cttcattgcc tataaacctt tatgaaagaa gagataacat
    22201 gcctactatc catattgccg gtgattctac tgcggcagaa aaagatgaaa acaagcgtcc
    22261 cgagacaggt tggggagaaa aaatctctgc ttatttttca cccgagatcc agatcaataa
    22321 ccaagcaaag aatggacgca gcaccaaatc atttatccag gaaggaaggt tagcccgtct
    22381 agcttcacag tttcagccga atgatttctt atttgtacaa tttggacata acgaccagaa
    22441 aatccaagaa gaaagaggta cgcttccctt cggtagctat ttaaaaaatc tgcagatatt
    22501 ttttgaatgc gcaaaaaaag cgaacgtcca aatggtctta ctgacatctg tgacgcgacg
    22561 agattactta gaaaacggaa cattgaatcc agatatactt ggagactatc caaaagcgat
    22621 gcgtgcattt gctgagaaac accatattcc tcttttagat gtgttctcaa gatcacaaga
    22681 attgttccag accttctcaa aagaagagac aaaaaaattc tacctgcatc ttatacctga
    22741 tacttgtaaa aattacccag aaggactaaa agacaatacc cactttagtc cagaaggcgc
    22801 tgataaagtt gcccgaataa ttgttgaatt gataaaagaa tcccgtccgc ctcttgctaa
    22861 ttatttacaa aaaggagttt gagatgtttc cattaaattt aggcatccgt gcccacgatt
    22921 tagatgttcg aaatcgtgaa gaaatcgttc aaaaagtatc acagcttcac ttgtcccata
    22981 tccaatttgc accacaaaaa gcatttcctg aacaacttaa attaactgac atgacacttg
    23041 ggagtgctgc ctattttggt gaatacttcc aaaaaaacaa tatcgaactt tccattttag
    23101 gatgctatat caacctatcc agcaaaaatc aagaagttcg tgcacaagca ttggcaactt
    23161 tcaaacagca aattgctctg tgctctgcct atcaagccaa gatgattgct acggaaaccg
    23221 gtagtgtctc tgtcggttac acaaaagaaa attttactga agaagcttat cagatcgcca
    23281 gaaattctat cattgaatta gtcgctcatg cagaaaattt tggcgtaact gttgctgttg
    23341 aagcagggat caatcatcca atctataact atcagttagc taaacgttta atcgatgaag
    23401 tagcttcacc aaatttaaaa atcattttag attgtgcgaa tttgatgcat aaagaaaatc
    23461 atgctgatca ggaaaaagtg atccgaggtg ccttggacaa tttacatggt tatattacag
    23521 cgcttcatct caaagactat atcatggaaa atggaaagat ccgcatggta cctgttggca
    23581 agggctgttt agatttccgc ccggtactgc attatatcaa atatgagcgt cctctaatgt
    23641 acgccactct tgaagctact cctgagattt atgttcctga agcaatagaa cacttgcagt
    23701 ttatttacca agattgttaa ggagaagatt atggaggaac gaatacagaa aagttatcaa
    23761 aaatatcatg caaatgcttt tcagacattg ctgcatcttt atcagggaaa ttattggcgt
    23821 tttgccatgt caggactctt ttttattatc aaacatcttc catcttggtt aatgccgatc
    23881 gctattgcca atgtaatcaa tgcagcttca tcgaaagatt cttctcagtt gcaggccatt
    23941 tatttcaacg cttttttgat ggcttgtctg attggtcaaa atatattgac gaattatttt
    24001 catgtaaaat accatagcac ttcgatccgt caagtagaag ctggactaag agcgaccctt
    24061 atcaagaaaa tccaagaact atctattcct tatcaaaaag aattgcaatc tggacgaatc
    24121 cagtcaaaga tcatccgtga tgtagaagct atccaaacgt tgtcctctca agtatttgtc
    24181 agcggattaa atatcgctgt caatttattc gtagctttag gtatcacgct ttataaaagt
    24241 cctgttgttt tcatgttttt cttattgact gttcctctcg ccgcattcgt tactctcttt
    24301 ttccgtaaaa ggatcagaca gtccaactca gacttcaggc aagaaatgga agagacttct
    24361 gcaaaagtga tcgaagtgat cgaattagca ccgattgctc gagctcatgg gttagaaaaa
    24421 cacgaaacaa ctcgcttaaa taagcatttt actcaaatct atgaaaaagg attccactta
    24481 gatatcctgc aatctgtttt cggatcagtc agctgggcta gttttcagat atttcagctg
    24541 atttgtttga ttttcactgg gatattagct ttacaagggc gcatccaacc cggcgatgtc
    24601 gtgatgtatc agacgtattt cacgacgatc gtcaattccg tttctggaat gatcacgctg
    24661 attcctacga tttcaaaagg catggaatct atcacttcta ttggcgaact gttgattgct
    24721 ggcgatgtag aaacttatca cggtaagaaa aaaatccaac atttggatgg aaattttcgt
    24781 tttgaggatg tttcttttct ttatccggac tcactcaaac cgattatcga tcatttttct
    24841 ctagaggtga aagctggaga aacaattgct tttgttgggc catctggctc tggaaaatct
    24901 acactcctta acttattgat tggatttatc cagccgactt caggaaaaat ttttctagat
    24961 gatcagcctt tggatgaact ggatatgcga agtgtgcgaa attatttagc cgttgttcct
    25021 caaaccactt tgttgttctc cgcttcgatc aaagagaaca tcacttatgg cttgaaaaac
    25081 gtatcaaaag aacgtttaga tgaagtcatc gaagctgcgc aactttcttc actgattgac
    25141 cagcttcctg atggtctgga taccttagta ggcgaacatg gcaacaagct gtctggtggc
    25201 caaaagcaac gtatctccat tgcacgagcg ttgatacgtc agccaaaaat cattttgcta
    25261 gatgaagcaa cctcagcttt agataaccag tcagagaaaa aaatccagca agccctgaat
    25321 tacctgacag aaacacccac tacttttatt gtggctcatc ggctttcaac gatcaaagag
    25381 gcagataaaa tcgttgtgat cgatgaagga aaaatcgttg aaataggtac ttacgaaaaa
    25441 ctgttagctc aaaaaggcta tttctaccgc ttgaaaaatg aatgacaaaa aataggagct
    25501 atgacaagac ttttgtcgta gctcctattt ctgaataaac ggtgttctcc tgccaactcc
    25561 tcggtcacaa gtcacaatag tcatcaaaca tgagaagctg ttttttgaaa ttgttccttg
    25621 taactcagag ttaaacggta ggctcacgac ctcttggtaa atggtgttca agagacagcc
    25681 tatcggcaat aagaagcaat tttgtcatcg ctcaaggccg aacgtctctt tcacaccctc
    25741 tgttgtttca taaaatagtt ttattcgctg taaaggtcgc gaactggtgt catgatgata
    25801 cggttcaagt cattgatgat tgtactgaag ccttgctctt tttgcatcaa gtcattgatt
    25861 acttcttctg attggatttt cacagccatt tcttgtgctt tcattgcatc ttcgtcgctg
    25921 aactcttgtc cactcatttg tttttcttgt aattcgattt gcatcgcttg gaagtctttg
    25981 aacagtttgt gtgcttcttc gtttgcttgt actttctcgt acgcttcttt caacgctttg
    26041 aattctggta attcacggat ctcacgttcg atttgattcg cgctatcgta gatgtttgac
    26101 atgttatgcc ctcttttctt ttagtagtta ctctctattg taacatgggg ttattcgttt
    26161 tcaaaggggc tactctcctt gatatttccg ccagaggtct ttagccgctt caccaattcc
    26221 ctgaacgcct tctttcacca tatcgccgaa gtttttcagc ccttcttttg cttgttcacc
    26281 atattgattc aagttatctt gccaatttcc agactctcca ttagaatttc ctgtgtttcc
    26341 aacttgatct gcagctacca cttgtcctcc tgtggcatag gcatctgcta cactaaatcc
    26401 tgtttttgaa gtatacggaa ggatgccttc tgccgctgat ttgaagactt tcccgacaac
    26461 atttccactt gtcccttcca aatagtgcag cttgctggat tcttcgtaac ccatccatgt
    26521 cgaaatcact acgtctggcg tgtaaccgat catccattgg tcatttgctt ttgtggcatc
    26581 gaaattagtt tctgtggttc ctgttttccc tgctatcgta taacctgcag gattcgcagc
    26641 agccgctgtt ccatttgaaa atgtaccaag aagcatgctg gtcatttcat cggctgtttc
    26701 ctttgatatg acctgttttg gttttttgtt tgtgttatcg acgatcaccg ctccagtcga
    26761 atcaacgatt ttcgtgataa gatgcggcgt atacatctga ccatcattgg caaatgcact
    26821 gtaagcaccc gccatgatca atggagatac tcctttttcc agtccaccaa gggctaagcc
    26881 ataatactta tcagattttg ctaaaggcag gccgaattct tctgctttat catatccttt
    26941 ttgtaaaccg atctcatgca aaagccatac agtaggcaaa ttcaaacttt gtgcaacagc
    27001 ttgatacatc ggtacttctc cttggtatgt cccgtcaaaa ttatgcgcat cgtaataact
    27061 ttgcggttcg tctttcaaga tactggaagg cttgtatcca gcttccagtg ctggtgtata
    27121 tacgcttaat ggtttgatcg ttgatcctgg tgaacgacgc atctgtgtag caaagctgaa
    27181 tcctctaaag acatgatctc ctcttcgtcc tactagtgct tgtacaccac ctgtattcgg
    27241 atctaaagct actgaagcag attggaccat ggcaccatct gaagcatttg ctgggaataa
    27301 tgcactgttt tgataagtag cttctaattg cttttgataa tcctgatcta aagctgtata
    27361 gattttatag cctttattca tgacatcttc ttcatcaaga ccatactcgt taactgcttc
    27421 atcgatcact gcatcaaaat aataaggata acgataacca gaatctgaat tatcgtatgt
    27481 gtcattaaga tacgcgctga tatctgtctg gctttctgct tgttcttgtt cttttgtgat
    27541 ttttccgttt tcttccatta cacttagtac tgtgtttttc cgatcattcg cattttttgg
    27601 gtgatcgatc ggattataaa taccaggacc tttcagcatt cctgccaaag tagcagcctc
    27661 agacagcgta acattcgttg catctactcc aaaatacttt ctggaagcgt cttggacacc
    27721 ccataccccg ttgccaaaat aggcattgtt aagatacatg gtcaagattt cttctttgct
    27781 gtacttcttt tcgatctcga ttgccaaaaa taattccttc gcttttcgat caaacgtttg
    27841 gtctaatgtc agatacgcat ttttggcaag ctgctgagtg atcgtacttc ctccgccccc
    27901 tgatgttccg ccagacaaga ccattcggac tgctgcacgg gcaatccctt tgatatcaaa
    27961 cccgtgatgc tcgtaaaaat tcctatcttc tgtcgagatt actgcatctt ggatataggg
    28021 agaaatcgcg tttaattcta catatgttcc tttttgggcg tacagtgtgc cagcttcttc
    28081 gtttttctta tcataaatga ctgtcgattg gcttaatcct gattttaacg tttcgacatt
    28141 tgcttgtttt gctaaaataa agaggtagat acttgtcact aagacacaga tcaaaccaat
    28201 cagcaaaatc aatttgttca catgatattt tttccagatg cgctttcgcc atcgatggaa
    28261 ttgtccgaga tatggtctga tccaacgcca aaaactgatc aaagcagctt ttatcttttc
    28321 taaaatgctt tgaaaatcca tttgcttctt ctcctgtcta tttgattcaa tcgtcttatt
    28381 ttaccatagt gaaagagtgt tttcttcctg cttaagttgt agttcaatat gtttttttac
    28441 gttagtgcaa gtagagtaac ataagaatga acagaaaaat gtaagttttt tcttaaatat
    28501 gttttaaaaa tggtacaatt agtctgcgat tgcactgaaa ggagtacact tgtgaaaatc
    28561 acgatcactt tacctatgaa ccatcctgaa acaacaattc gcgacttgct tgaaaaggaa
    28621 tggttagttc ctagaaaggt tcgacacttt ttgcgtatac gtaaaaacgt ttggatcaat
    28681 caacaacctg ctttgttcca tcatgaagta aagggcaatg accagatcac attgttattc
    28741 gaagaaacag attataatta tcaagaagtt caattaggag atgcgaagca catccgtgtt
    28801 ttatatgaag atgagcattt gattatagtg gataagcctg caggaatgaa gactcatcca
    28861 aatgaaccga cagaaaacag taccttactc aatcatttag ctgcttatct cgacactaaa
    28921 cagcaacgac cttacgttgt ccaccgcttg gacaaagaaa caagcggtgc cgttttattt
    28981 gcaaaaaatc catttgtctt gccgatactt ggaagaatgt tggaacaaaa aatgatctat
    29041 cggagatatc aagccatcgt atggggagaa attgccaaag atatgaccat caccaataaa
    29101 attggacgag atcgccatga tcggagaaaa agggtcgtag atgataaaaa tggtcagagc
    29161 gccttgacac atgtcactgt caacaaagta ctttctgaac aaacacaagt ctattgtgtt
    29221 ttggaaaccg gacgtacaca ccagatcaga gttcatttag ctcataaggg acatccgatc
    29281 gttggagacc ctctctacca aaatagaaca gctgaacgac tgatgctcca tgcattagaa
    29341 ctgcacatga tccatccatt tactcaagaa aaaatagtcg gtaaagcttt acctggatta
    29401 tgggaataga aataaaaaag aacagagtct tatcagcaga taatgataag actctgttct
    29461 ttttgtgtaa tttgtctgct tacttttgat gttcgtgttc tgctaacagc aaactaccga
    29521 taatcccact ttcattcggc agtccccaac gaacgatata ttcacttact tgaggtgttt
    29581 ccatataacc acccatcaat tcaacaaatt gctgacggat tttttgaagg agatgatcct
    29641 gattcattac cccaccgcct aaaataatct tttcaggtgc taacgtcaac gtatagttga
    29701 tcagtgcttg agcaatataa taagcctgaa tagcccaaac aggatgatct tgcggcagat
    29761 cttgcccttt gatccctgtg cgtgcctcaa tagacggtcc tgctgctaat ccttctaaac
    29821 aatctttatg ataaggacaa gtaccttcat aatggtcatc tggatggcgt ttcacccaaa
    29881 tatgtcccat ttcaggatgt gcgatccctg aaaaaatttc ttcattgatc acgacacctg
    29941 caccgattcc tgttcccacg gtaaggtaaa tacaactatt ttttccttgt gctgctcctt
    30001 gcttcaattc cccatatgca gcagcattga catccgttgt ccaagcagca ggaatattgt
    30061 agcgttcctt gattgtgcca agaaaatcga attgtgccca cccttttttg ggagttgcca
    30121 aaacgtatcc gtaattatct tttgttttgt cgataccaat cgggccgaat gagccaatcc
    30181 cgattccctt caattcataa cgatcaaaaa attcaaatac ctgatccaat gtttcttctg
    30241 gtgttgtggt agggatactt aatttttcta aaatttctcc accatctgaa accgcacaca
    30301 caaattttgt tccgcctgct tctattccgc catacattcg ttttcctcct cgaatccgat
    30361 tgtccgaatc tcataaggtt ggattattgg gacaaccgct tgcttcgttg gttcttcaag
    30421 cagattcaat atgacagttt ttccattgtc tttagttatt tccaattttt ctaaatgact
    30481 agacatattg aaacctctta ctactacttt atcactaaac ttgctgcgtt tcaaagctgt
    30541 aattgcaaac gtttcacttt ttatcgttaa atattgttgt tttgaaatca atgttccttc
    30601 atgatgtttg atttgttgag tggaaaaagg aatctgtgct gcgtatgcat gcaaataagt
    30661 agagaatttt tcttctggtc catgtagttc aatgctgtaa ttgaaccgat gctcacctag
    30721 acactgtgct tctggtgttg gaaaatatcc ccaatcgcct agttctccca cactgcgaag
    30781 tagcgtcact gcgatttgtc cgtcttctgt tacttcatat tcattcaaac caaagtttcc
    30841 gactgttaca ccgtactctt catcatgaat attgacaaat gcttgctgat gttgcggatt
    30901 cgtggggttt tcccaacttt tagacacatg gtttgggcgc ttcaccactt caaaaatact
    30961 atctgcttcg tgatgttcca catgtaactt tgtaggaaac aagacacgta agcgatgatc
    31021 tttcatctga ttgtcgagag tcgtttcaaa gtcgattttt ttactatctt ttctgattgt
    31081 catgattgtt tttatttgaa ggattcgttt ttctttggat cgttctgctt ttcgataacg
    31141 aaattccaca accatctgct gttctttttc taaacgctcg tctgcagaaa cagggatttc
    31201 catttcctgt agaatactta tttttgctat ttccggtgta tctactaata cttttacttc
    31261 agagttttct ttgttactgg ataggattgg ctgatcacag aaaggttgtt taaagatata
    31321 ctcatttcct atatcgccga tatcttcaaa aatatgcaga ttattcaact ttcgtttgtt
    31381 tttgcgatct tccattgtaa tggtaccatt tttttcaatc gtcaaatgta gagggccgtt
    31441 ttcgatgatt cgtccactct gatgaatcat tgtttccttt tcttctgctt tcgcttcccc
    31501 ttctacaagc gcgaatgttt cccaagaaaa tgctggcatg ttttctaatg gtaaagtcac
    31561 tttgatcatt cttgccatat atggcacacg gaaacggtct ttcggcaaat cataaccaaa
    31621 ttggacagtt tcttcagata aaatagctgg tatagtggcc ccactttttg tttctacatg
    31681 atacttacgt ttaggcaaat tttctaactc ttgatatagt tgttcaggta ttccttcagc
    31741 gaatcttttt ctttctaatt cgatggttat ttctgcttca ccagttttat ccattcctgc
    31801 agtatttaca atgacaaatg gaaagctgtc ttttgggaat ccggtcgtat cgattgctgc
    31861 ggtcagctgt tctaaggaat cttgtgcaag atactttcca acttcatctg ctttttcaaa
    31921 acgaggtatc atttcccgat ggacagaatc gactgaacaa ccgcaaatac tgtcgtgagg
    31981 atgattttgc attaatgtct tccaagcata atctaattga tcatggggat agtttccact
    32041 aacttcataa gccatcgtcg caagtggttc tgtaatattt tctagctgtc gctggacctt
    32101 ggtattccat tgttttaagt aaacccgagc agatgctgta ttggctaaag tataccagcc
    32161 atccgtttct tgacttgtca attctccttc tactgagcct aagtcttctg gcacatcttt
    32221 ttgaactgct tccagatagt cagtaaaatt agaatgaata aactcgtaat ctggaaacag
    32281 ttcatttgcg agatggattg cttttgatat atctttttgt accggttgat gatcaacacc
    32341 attcatcatc aataagtggt tcgtagaggc atattgttct gcatccgcta atttttgctt
    32401 ccaaaaggca agagctgctt ctttttctac tggaatctca ttcccgttgc tataccaatt
    32461 ggcaaatagc agaccaaaga ttgctgtttg atcaggtcct ttccaccaca tctctgagta
    32521 ttgggaagaa tagttttctg cttccaaaac ttgattatca aagccaattg gttttacacc
    32581 acggccaaac gctgctgctg aaataccagc ttgtttcatt aattgaggcg tctgtcccat
    32641 atttccaaat gtatcgggaa aatagcctaa catgacaggg gttccccatt tacggctttc
    32701 ttccatgcca atcagcatat ttctcacgtt cgattccgag ctgataagaa aatcatcttg
    32761 caagatataa aatggtccaa ttctcaattt tccttcattg attgcttgtt ggattgcctg
    32821 tcttttttct ggccttactt gaagatagtc atctaaaata atcgtttgcc catccaaatg
    32881 aaaactgttg aaggacggat caacttcaaa tagctctagt agatcgtcta tcaattcaat
    32941 cagtcgcatg tgatgctgtt cgtaagccat gtaccattca cgatcccagt ggctatgaga
    33001 aatgatatat actttctttt tcatttacga tacctcctac ttttctactc taatatcaaa
    33061 gtaatccatg actaattcgc aaaacatcat atttgcccaa gaaaaccatt ctctcgtata
    33121 attttcagga ttgttcacat cgaatccttc gtgcataagg tgtgttcctg cgtcagttgc
    33181 tacaagtaaa ttcaatatcc gctctttttc agatttgtct gacgttgtca ttccttccat
    33241 agctaatgca atcggccaaa cgtatttttc tggcgtatgg gaggatccaa ttccttttgc
    33301 gtactttcct tcatagaaat atggattttc tttactcaac aatgttcttc ttgtttgcag
    33361 ataatcttcc tcttgttcag aacaatatcc caagtaaggt gcggcgatca gattgggcac
    33421 attactgtca tccatgactg tggcgtttcc cattccatct acttcatatg cataaatcgt
    33481 ttgccctttt tgattaaccg ttcgtccgtg attctgaatc ccctcttgga tctctttctt
    33541 caatttctgt gtttgctgta caatttcttt atcatcgagt atcgtttgaa aaatttcttc
    33601 cacataacct aacacgacca ccgcaaacat attggatggt accagatatc cataacggca
    33661 agtatcatcg ctaggacgga aaccagacca tgtcattcct gtaaaagctg tttttgcacc
    33721 tcgcccgtca ttgatcagcg tatcttcttt tcttgtcgtg tctcgtacaa atgtataagg
    33781 agagtgttca tgatcttgct ctgttttgaa cacgtccaag atttttttga taccttcaat
    33841 aaaagattga ttgaattgat cagtttttcc tgtatttttg taaagaagat aggccaactg
    33901 tattgggtag cataaagaat caatttcata ttttctttcc cagatccaat cgttcatttt
    33961 tgtatgatct gtttggtggc cagcaccgtt agcttcttcg ttgaaggcat tcgcatatgg
    34021 atcgatattg atataaaaaa attgcttttt gactaacccg ctgatcatgt cagctaattc
    34081 ctcatcttct tttgcaatca ctaaataagg acgaacttgt gcagtagagt ctcttaacca
    34141 catcgctgga atgtctcccg tcaataaaaa ggtggttccg tcattttgct ttttgactgt
    34201 tgtcaacaga gtattggcaa atgctgctgt aaaattcttt gcccagtccg catggatatc
    34261 cccacatttt tgtgtgatat cttccataaa tttctgcacc gattttggta tatctttata
    34321 agccactaat aaaaccttct ttctatttga tatatcttta aagattatac ttcatacagc
    34381 caattatgtc tacgctttca aaaaatatct ttttatttac tttttcatcc attatggtat
    34441 tatttgaata tgatatatca ttaagaggtg tttatatgaa tcaaccattg taccagacaa
    34501 tatttaatga tttgcaaacg gctattttaa ctggtgaatt acctgtagac agccaactac
    34561 caactgaaaa agaactttca cgtacctata aagtaagcag aatcacttcc aaacgtgcat
    34621 tgactgaatt agagcaatta ggacttattt atcgcgttcg cgggaaaggc agtttcgtta
    34681 aatccgcttc tccggaacag attcttgctt ctcctacaaa aacacgacgg attctgtttc
    34741 ttcttccata tgtcgctgat ttgtctgttg gtgattttag caagggattg aaccctgtga
    34801 tgcaagataa acagattgat gtcatgatga cgacgcttga ctttctacaa aataaaacag
    34861 caaaagagct aatcaatgaa tttgacggat tgatttatta tgctcttgat acagaacaac
    34921 atcttgactt actttttgag ctttcattga aagaattccc tgtcgtcata ttagataaaa
    34981 aaatctatga gttacctttt cctaccgttc tttccgataa tttccaaggc ggtgcgcttg
    35041 ctgcctctta tttgatcgaa cagggacatt caaaaatcgc ttatttgttt ggtacgcaga
    35101 ctcatccaca atccgttcga cagcgttacc ttggttacct tgaagcattg gacaaagctg
    35161 gtttgacttt ccacacttca ctggatgaca aaagcgcgac attagatgaa ttgctttctt
    35221 atatcactaa gtatgaagta actgcttttg tttgtgaaaa cgatgttgtc gctatccaga
    35281 ccatgaatct cttgagacag catagttatc atatccctga agatttttca attatcggat
    35341 ttgatgatat ccaagcagca gctcttgttg acccgccact gacaaccatc gcacaagatt
    35401 ttgaacagtt aggtaagctt gcgggagatg ctttggtcac ttggttagag acaaaagatc
    35461 gaccatccga tagtaaattt ccagtttcat tcatcaaaag acaatcaaca aaggagaaat
    35521 aaaatgaata tcggtcaaat agatactaga catgcaacag cgaatcagcc aaatttctct
    35581 aatgggaact gccagcctta tactggcgtt ccttttggga tgaattattt tgcaccgcaa
    35641 actacggatc aaaacggcag ctggtggttt catcctgatg accgcgtttt tcaaggctac
    35701 cgtcttaccc atcaaccaag tccttggatg ggtgatttca gtcatatgct gtttacgccg
    35761 atcaatggaa aactacagga gaacaccctt ttccatgctc aaagttctta ccgcccagaa
    35821 gaaagtatat tttgcccgac tcatctgtcg atctgtcaat tacgttatgg catctgttct
    35881 acattgatac ctagcatgta tggcggaatc ttatctattg attacagcaa aaatgacagc
    35941 ggtttacttt tatcctttcc gggacgccat cagctctttg ttatagatcc ttacacagtg
    36001 gaaggaaaaa tcacacattt tgctggttca gaagatcctg atttttcttt ttattttata
    36061 ttgagatttg aatatccttt aacaattgat gagttagctg tttccaacaa aaatagcgac
    36121 tcgatcccgt tgtattttga acagacaaaa aaacaaacta tccgatttgg gacatctttt
    36181 atcagctcgg aacaagcagc ttataacctg gaactagaac aaaaccaaag caaagagact
    36241 taccttgctc acagtcaaga acaatggcag tcctattttg atcgtataca gctcacacac
    36301 catgatcaac aacaattgcg gacgttttat cataatttat atcgggtgtt tttatttcca
    36361 caaacatttt atgaaattga tcctagtggg caaaaaatcc actatgatac gactagtcga
    36421 tctataaaac ctggtgttct ctatacaaat aatgggtttt gggatacgta taaaacggtt
    36481 tatcctcttt attctttgat tgctgtagaa aaatacgaag aaatgctgga aggttttttg
    36541 aacagctata gagaaagtgg ctatttgcca aaatggctgt ctcctgatga acgtggactt
    36601 atgcctggga cattgatcga tgcggtcatt gcagatgctg ctgtaaaaga tatccgacga
    36661 gacttgatgc ctgaatttct aacggcaatg gaaaaaggtg ctacggttca aagcaaacat
    36721 ccaaattatg gacgccaagg aacagaagat tacttgacat atggctatgt tcctaatcac
    36781 taccacgaat cagtcaatca cacattagac tatgcttata gcgatttttg tattagccaa
    36841 gttgccaagg tactagaaga agaggaaatg acgaagaaat atcagcagca atcattgaac
    36901 tatcaaaata tctttgacta tgagtcagga tttatgcgtt caaaagataa agatggcaag
    36961 ttcagagaac cattttcagc taatcgttgg ggacaagatt atgcagaagg aagtgcttgg
    37021 caaagcagct ttgctgttta tcacgatttt tcaggactga tcggaaaata tggtggcagt
    37081 gagagatata aagaaaaact cattgagctg tgcaatcagc ccccaacgtt tgacccagga
    37141 ggatatggtt ttgagatcca cgaaatgagt gagatggccg cgatagaatt tggacaatta
    37201 gctatttcaa accagccaag ttttcactat ccttacttgt tcagttatat aggacaacct
    37261 gaaatggcac agccactgct taaacagttg atgactcaga ccttcgacga ttctccaatg
    37321 ggttaccctg gagatgagga caatggaagt atggccgctt ggtatatctt caatagtctg
    37381 ggattttatc ctgtcacgcc tggaaccggt gaatatgtta tcggtatgcc attaattgac
    37441 aaggctgttt tgcttttgtc aaatggaaag gaattgacga ttgaagcaac gcctaatcgg
    37501 ccgcagcaac aatttatcca taaaatcaaa cggatggaca aagaatatac gaaattatat
    37561 tttacccatc aagatttgct gaaaggcggc acgatctctt accaattagg gatcatccct
    37621 caccctaaga aatatcaagc aaaagatttg cctttttcct tatcaaacta taattaacaa
    37681 gaggttgtga aaaagctgtc ctgcatcaag taataagaac agccaagcaa aa
//
LOCUS       DAMEET010000015        37143 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.015, whole genome shotgun sequence.
ACCESSION   DAMEET010000015 DAMEET010000000
VERSION     DAMEET010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 37143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.015"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(98..1228)
                     /locus_tag="PB780_000679"
     CDS             complement(98..1228)
                     /locus_tag="PB780_000679"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDE0732751.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..3079)
                     /locus_tag="PB780_000680"
     CDS             complement(1877..3079)
                     /locus_tag="PB780_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDE0732752.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="PB780_000681"
     CDS             complement(3139..3342)
                     /locus_tag="PB780_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HDE0732753.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="PB780_000682"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="PB780_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4879..5691
                     /locus_tag="PB780_000683"
     CDS             4879..5691
                     /locus_tag="PB780_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDE0732754.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6715..6867)
                     /locus_tag="PB780_000684"
     CDS             complement(6715..6867)
                     /locus_tag="PB780_000684"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDE0732755.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7058..7936)
                     /locus_tag="PB780_000685"
     CDS             complement(7058..7936)
                     /locus_tag="PB780_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDE0732756.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7975..9093)
                     /locus_tag="PB780_000686"
     CDS             complement(7975..9093)
                     /locus_tag="PB780_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HDE0732757.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9090..11189)
                     /locus_tag="PB780_000687"
     CDS             complement(9090..11189)
                     /locus_tag="PB780_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HDE0732758.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11240..12061)
                     /locus_tag="PB780_000688"
     CDS             complement(11240..12061)
                     /locus_tag="PB780_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDE0732759.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12058..12867)
                     /locus_tag="PB780_000689"
     CDS             complement(12058..12867)
                     /locus_tag="PB780_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDE0732760.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12888..13382)
                     /locus_tag="PB780_000690"
     CDS             complement(12888..13382)
                     /locus_tag="PB780_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDE0732761.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13394..13882)
                     /locus_tag="PB780_000691"
     CDS             complement(13394..13882)
                     /locus_tag="PB780_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDE0732762.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13959..16655)
                     /locus_tag="PB780_000692"
     CDS             complement(13959..16655)
                     /locus_tag="PB780_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDE0732763.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17083..18156)
                     /locus_tag="PB780_000693"
     CDS             complement(17083..18156)
                     /locus_tag="PB780_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDE0732764.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18153..18980)
                     /locus_tag="PB780_000694"
     CDS             complement(18153..18980)
                     /locus_tag="PB780_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDE0732765.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18981..19787)
                     /locus_tag="PB780_000695"
     CDS             complement(18981..19787)
                     /locus_tag="PB780_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDE0732766.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19799..20884)
                     /locus_tag="PB780_000696"
     CDS             complement(19799..20884)
                     /locus_tag="PB780_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDE0732767.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20901..21443)
                     /locus_tag="PB780_000697"
     CDS             complement(20901..21443)
                     /locus_tag="PB780_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDE0732768.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21701..22690
                     /locus_tag="PB780_000698"
     CDS             21701..22690
                     /locus_tag="PB780_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDE0732769.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22687..23424
                     /locus_tag="PB780_000699"
     CDS             22687..23424
                     /locus_tag="PB780_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HDE0732770.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23421..23972
                     /gene="coaC"
                     /locus_tag="PB780_000700"
     CDS             23421..23972
                     /gene="coaC"
                     /locus_tag="PB780_000700"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HDE0732771.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            23988..24620
                     /locus_tag="PB780_000701"
     CDS             23988..24620
                     /locus_tag="PB780_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDE0732772.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24705..25352
                     /locus_tag="PB780_000702"
     CDS             24705..25352
                     /locus_tag="PB780_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDE0732773.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25357..26340
                     /locus_tag="PB780_000703"
     CDS             25357..26340
                     /locus_tag="PB780_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HDE0732774.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(26743..28257)
                     /locus_tag="PB780_000704"
     CDS             complement(26743..28257)
                     /locus_tag="PB780_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HDE0732775.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            28395..29201
                     /locus_tag="PB780_000705"
     CDS             28395..29201
                     /locus_tag="PB780_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732776.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            complement(29376..29945)
                     /locus_tag="PB780_000706"
     CDS             complement(29376..29945)
                     /locus_tag="PB780_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDE0732777.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(30028..32088)
                     /locus_tag="PB780_000707"
     CDS             complement(30028..32088)
                     /locus_tag="PB780_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HDE0732778.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            complement(32365..33843)
                     /locus_tag="PB780_000708"
     CDS             complement(32365..33843)
                     /locus_tag="PB780_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDE0732779.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(34141..35076)
                     /gene="murB"
                     /locus_tag="PB780_000709"
     CDS             complement(34141..35076)
                     /gene="murB"
                     /locus_tag="PB780_000709"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDE0732780.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(35149..36069)
                     /locus_tag="PB780_000710"
     CDS             complement(35149..36069)
                     /locus_tag="PB780_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDE0732781.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            36182..36934
                     /gene="xth"
                     /locus_tag="PB780_000711"
     CDS             36182..36934
                     /gene="xth"
                     /locus_tag="PB780_000711"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDE0732782.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT        11105 a         7691 c         6272 g        12075 t
ORIGIN      
        1 aataaataat ataaaaggta aataaaaaag agtggatttc tccactccat gaaaagccga
       61 ctttttggga cgcttttttt tgccaagcct cacattttta tagccaaact tcttttgcga
      121 cttgctgcaa taattggatt ttcttccatt gttcttcttc tgttaaatgg tttccttctt
      181 ctgttgaagc aaacccacat tgaggactga tacttagatt ttccaatggt acaaattgtt
      241 gtgcttcttt tattcgttct tttattttct ccgtatcttc caaatctgga aacttagaag
      301 taactaatcc taaaacaact ttactttctt tgttactcca atgtcttaaa ggagtaaaat
      361 ctcctgctcg gtcagaatcg tattccaaaa agaaaccatc ataatttgtt gaaaacaact
      421 cttctgcaat gggttcatat ccaccggaaa acagccaaga agaagcgtga ttcccgcggc
      481 agatatgtgt tgtgatcgtc aaatcttctg gtttgtcttc cagtgcgtta ttgataatct
      541 ctgtacaaat atgggccatt tctttcggat caattccttt ttgaagaagt gcttctcttt
      601 tagtttgatc agctagaaac gcccaatttg tatcatcgaa ttgcaggtaa cggcaacctt
      661 tttcataaaa agcatgaatc gctgctttgt aagtagagga caagtcctca taaaaatcag
      721 atagatcagg ataaatctcc ttgatttttg aagaaccatc attagatagg atctcttgtc
      781 gtaaaatcat cgtgggacta gggatcgttg cttttgcaac aaaagtatca tctgtttcca
      841 ctacttcttt cagaaaagca aaatctttta aaaatgggtg atttggattg aaagataatt
      901 tatctactac ccgaatatta taagctggtg ctgcttttcc gtggaatgtt tgattatatc
      961 cagtttctgg aacatagcct tcgataccat ttagttcttc taaaaaatca agatgccaaa
     1021 atccacgccg aaattcacca tctgtaattc ctttgatgcc gactgctttt tggtcttcca
     1081 ctaatttttt tatttcctgg tcttcgattg cacgaagttc ttcttttgaa agtaagcctt
     1141 cttgaacttt ttttcttgct tctttgatcg attgcgggcg taagaaactt ccgacatgat
     1201 ctactgtaaa tggtctattt ttgctcattt cactcactct tttctttgtt ttttgtaaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta gaaccggggg ctttattcct gcagtccggc
     5821 tacgccttcc tgcaatagac aaaactccag atcgggtaat tcgcttgtgg cgaaacccga
     5881 cctggagttt gtctctgtat tttgaaccct aaaaattaat caaacagact gatattctca
     5941 taatcagcgc gaaataagcc attaagtaca gttttcctac ctatttttga taacccgctg
     6001 gttagttaat attccttttc cgagagagat aaatgattgg ttaaatctat atacatctca
     6061 acctttaaaa atgttttcga aatacttcat cagtacatgt catacaaatt accagtgcat
     6121 tctcaaattc tagttcttta attgatttca gttgaggagc agttactttt ggcggaaatt
     6181 tccatttttg tccaaacctc ttcccccgat gaaatttctt tgttgcaatt attgcaatga
     6241 aaaatttttg tttcaaaaat atacataatt acttcctcca ataaaaacta tctacctctc
     6301 aaattatttt tctctaataa gaaaaacaga aaaggtaata agatacaaca gtgatgattc
     6361 ctaataaaga aaagactaca ataatatttt tctaacttat cgccttcaaa gaaatagttt
     6421 cttatttttc actaaaataa aaaatttatt gcaaaaaaaa tagaagtgac aaaattatca
     6481 atcatttcaa ggtaatataa ctacctgagt ttattatcga ttaaaatgct aactacattt
     6541 gcaagagaag cgagagttaa aggtaaatat agaatttcta cttttttagc aatagaaata
     6601 ggaataaaat atttaaaatg tatagaaact ttcttttttt ttcttgctat ctttttataa
     6661 tcacaactct ctataataga attacgattt attttatacg gctgaaaaaa tctatcacca
     6721 aaaaaattca aacattaagc caaggattat gaagtcaaca tttcgaaaaa tgaacataca
     6781 ttggtaagca actataacta ttaccacaga cataaagtaa cacaatatat tactatttaa
     6841 gtgtacgcca ccagtgaata tcctcaaggt atacagcgca caacaaaaag aaaaaaggaa
     6901 acgcagaagc atttcctttc agtgatttaa tgaaaacaac acgatttgac ttagagcctt
     6961 tttatttgaa ttcattttcg ctttatccaa cttcatttga tcatttgatt actcggttaa
     7021 atacttttga catgagtatc aatgaaacat tcattaatta tggttgctta tttcttaccg
     7081 cttttctggc caatgctaat ccgccgatta ttccggcatt atcacctaat tcgggcgtaa
     7141 taatgtagct atcaattggt ggtatttcaa catatccatt tataagctca acaaatttat
     7201 cctgaacttt tttcttcatg tgttcttgtt tcattacacc accgccaaaa ataattctat
     7261 ctggcgaaaa catcagtgtt gtattgtatg cacattgggc aaggtaaaag gcttcaatct
     7321 gccaaaacga atcgtcagcc aataaattct ggccttttac acccaaccgt ttttcaatcg
     7381 ctggtcctgc tgccatcccc tctaaacaat cttggtggaa gggacagttt cctgaaaaat
     7441 tgtcctttgg atgtcgcttg atcaacatat gccccatttc cggatgactg aacccttcca
     7501 cgaaagtccc attgaacaag gcaccacctc cgatacctgt tcccacagtg aagtatgcaa
     7561 cacctgctaa ccctttgcca gaaccagcaa catattcacc atagcaagcc gcattgacat
     7621 ctgttgtcca ttcaatcgga atattgaaat attgctttag cgttcctaca aaattgaaat
     7681 tttgccaagc aagttttggt gttgatgtga tatgaccgaa actcgttgat ttgcattgaa
     7741 tatcaattgg accaaagcag ccgaccccga tgcctgccaa ttgttcctta aaaggtttaa
     7801 aaaaatcaat gacctgcttc attgtttcaa ctggagttgt tgtgggaaaa ctgactcttt
     7861 ccaaaatcgt taattcctca gtaccgactg cacagacaaa tttagtccct ccagcttcaa
     7921 ttgccccata tatcatcata ggttcctccc ttcgaatatg tgaaaaacac ttcattaacg
     7981 cggcaagcgt tctcgatcag tgaaatgacg tttagtattt cctgaagctt ctgttgaata
     8041 ccagtaagcg acactagcaa catcgtcttg ccgctcaaat aagcgaacat catcgttgcc
     8101 gatttgttgt aaagtgaccg ataaatcttc ggtaaaacag atggggtctt gaagatgcca
     8161 acgataaaga ccatgtcgtg gcattgcgtc gtcaccaaag gcatgaacgg cattcaattt
     8221 tcccgtttca aaacgatctc ttgtggcatc tctatttgat tggtaaggat accctaaaaa
     8281 ggctgttgaa tagtttttcg catgtggacg tccgaactca tcgtactcat gaaaggccca
     8341 cgccccgcca aaataatctt ctgaacctgt tgaggttacg gtcggatact cactatcccc
     8401 atcaatgtag aatttaaact cgccttcgcc ccaccaataa cgttctaggc aggtaagcgc
     8461 aaggtaagta cccacgtaat agccttgacc ctttaatcga tcaattaact tataatcctc
     8521 ctgtaatttg gtaatgcgtt cccgatgcca atttgcgtga aaatataggc tattttcttc
     8581 caattcgtcc accaaagcgt aatttatcgt ataaaaaaat gaggacatat ccttcggatg
     8641 taaattctcg atcgtaattt tagcggatgt ttggaaaggc atttcgaaat aggaattaaa
     8701 gccccctgta ggattaacca ctatcggcaa cgagttcacc tcgcaacgct ctccaaaccc
     8761 attacaaaag aaatccccta aaggactttc aacagcaggg ctatccgacc catcccaata
     8821 aattcttaag attaaatcac gtaaaacaaa actgccaaat tctgttcgat cacgaatcgt
     8881 catccacata tgacgaatta ttcccgttcc agtaatatcc gcaatcacag tttcttcacc
     8941 agaagtcagc ggaattgcgc cacgaccttt gcggcttggt cctagctcgc ttggcaacat
     9001 cgcagcttta cctttctcgc ctgttggatt ttcgggagtg attgctcgac tctggatttt
     9061 tttaaattgg gtaatatttt ttagtatact catctttttc tcctttgaat gtgaatcgtt
     9121 tttccctttt ctacgtgaag aatttttttc gtatcactta ttttcaatgg cttttctgac
     9181 aaatttgaaa tactcaactc ggattgcgta cattcaatcg aaatgaaggc ttgaccgaac
     9241 ttcaaattct tccaagaaaa cgttaaatta tcaacaggat caatttgcaa tactcctttt
     9301 ttgaatttca atccgaaata ttgactgatc attaggctga caaaaagacc cgatgcccag
     9361 ccgcatttgc cgcagccaaa gtctcgcacg acttctcctt gttgagcctc taagcgatac
     9421 ggccaccacc accacgaacc gtctaaatct gccaaacaaa ttagttcttc taatcgggtt
     9481 acgaaagctt gagggttgtg cagctcgctc attaaaacag tgataaagcc aggaaaagtg
     9541 gcgccggatt ctaatcccca agtgatgcct ttgatttcct cgctataggt tggattgaaa
     9601 gaactagcag aaaaggccat ggtgttctga tataaatgat ctttattatc taaaaattga
     9661 taaaaaggca tcaacgtagt gtctgattct tctccatcat gcatcaataa attaattttt
     9721 ccatccacaa tcacatcact taaaaagatt aacccttgct caagaatcgg tttttgatag
     9781 tgcttcactg aataggttgt tttctcctca tcaccgattc cctccaaaaa ttgctcacca
     9841 aaaggaccat cgatagtcat ttgtttacaa attgattcct ttagtttaca ggcaatgttt
     9901 ttgtatcgct tgctaagact cagttgttct agggcttcaa aaatttctgc aagtccaata
     9961 caggccttcc aaagacaaag attgctgccg gtatgatact ttccaagtgc aaaggcgtct
    10021 gataaccaaa cagatggaaa aagcattggt tcccctgacc gacgttgagc taatatgtcc
    10081 tcaataattc ttgaagcatt cagtagaacc tgcggacacc gctcaaagaa ttttgtatcc
    10141 ccagtatgtt caaaataaag actagccagt aacgcactgg ataacgaatt actcagagaa
    10201 tgattgatac ctcctgtaaa ttttgaacca ggaaacttaa cgccatattg atcaaaccat
    10261 agaaccgttt tttgacataa ctccggttca aacatggtga atggcaatga tgcgtaatac
    10321 atatccttat tccaaattcg attagtagca ggatagctgc cccaattgct gccgacgacc
    10381 tgtccctgtc gattcatacc gaaactagaa aagctttgat aaagtgcacg acgatagaga
    10441 tggacaactc tatcgtttgg cattgacagc tgcccaaata gctgagcaaa atagttcaat
    10501 gtttccttca gccatttttc gatatcagat tcctgaaaag tcattacttc ttcaaaagca
    10561 tttgggtcca caaaagcaat ggcgaagctt gccacttcct gttgtgcgat cgtgtcctta
    10621 tttccttttt gagaaatcaa cacattttgc tgatccgaat acttctcttg atacgaaggc
    10681 acatctttca ctgaaacttg aaggctttcg tgtgtttcat tcacaatgta aacctgttga
    10741 acaagcatac ttaaacgctt attagccagt atgggagcaa aggatactac aatgacctta
    10801 aacgtcgaat aatgatgaat atatctaggc aaacagtctt gaatcacatc gacttcaata
    10861 cgatgatccg attctgacaa atggtagaca atcccgttta cttcaacctt taaggataac
    10921 gaattggttt gagagagctg tttattgacc caaacacctg gtttttcctc agttagtaaa
    10981 ttttctcgat aaaaggtcaa cgtttgaatc gtcccgcaaa catcaaggtt tgctaatgtc
    11041 ttttgattac ttacatcgaa ggtcaatccc tttacatcag gaatattccg atagtagctg
    11101 tattcgtttg aagtagtcac atttccgtcg tttaactcag cattaaaaaa tgggtgtttg
    11161 ggtgaataaa ttacttcatt aatagtcatc ttttccctcc ttgctaaaaa aactgcagcc
    11221 tagtagctgc agcttcctac tatgcgattc caagtaatga caataaaatt cccaagacga
    11281 ccattccaaa aataattttg atgacgccga cttttttatc tagtaatttg aaacagctca
    11341 atgttaccaa taacggtaca attcctttaa aaatactatc gaggatttct tggacattca
    11401 ttagttcttc gcccttcatt gaaaagctca aaacggtatt gaatttgacc atactgatcg
    11461 tcattgcacc taccatgatc agacccaaga tactagcagc ttttgtcaga atttcaatta
    11521 acccgttcgc atacagcttt tcaatatatt tagagcctaa tgaatagcct aaaacgccgc
    11581 catagtatcg catcaagtaa ttaggtatat tgaacataac taaaaagata attggtgcca
    11641 aaatatttcc ggcattggct aagttgatcg ctagtcccgc ggtaacgaca cgccaaactc
    11701 cccagaacaa tgaatcgcca attccagcca atggtcccat caaactcgtt ttgatcgcgt
    11761 tgatactttc cacgtcaaaa ccagcttttt cagaattttc cttctccatc gatgccgcaa
    11821 tccccatgac aaaggacgcg caaggaatgg tcgtcgaaaa atactgtgta tgacgttcca
    11881 gcgccgcagc tcgatcttct tcacttttgt agaaatgatt aatgaagggc atcatagaat
    11941 aggcgaaacc accgccgccc atcgtcaccg gattcaccga agcatacata gtcatcgagc
    12001 gccaaaacat cttccgaacc aattttttct catctttaga aattccatca gctaatttca
    12061 ttgaaagaaa tcctcctctt cttggtcaag cgttgcattt acagcagcat cgctttgaac
    12121 tgcatgaaca gatatatttt ttagttgtag atctctttgg gctataacaa ttgcaataat
    12181 cgttccaact gcggcaattg caatcaatgg taactcgaga tacgccataa caacggcccc
    12241 aaagaaaaac cacacagcaa tatttttact ccaaagcatc cgtaacagca tcgccatccc
    12301 aactgccggc agcagatttc cagcgactgt taatccgttc atgatgacct ctggaatatt
    12361 atccaagacg cttttgacaa catctgctcc aaataaaacg gcaaagaatg gaataagagc
    12421 ataaagcgca tagtggacaa accacataaa gaagtgcaga ttgatgattc ctttttgatc
    12481 accagatttt gcaaatttga aaaatttagc agccatcggt ccacatacaa aagaaaaaat
    12541 catattcatt aactgtaatc ctaatactgc tagtggaaag acaatgggaa taatcgcatc
    12601 cacgtttcct cccattttga ttgcgaacgc tgtagccagc ccagcagcca aagaaggctc
    12661 cgccgtacta gagattccga tattgacgac acccataaag atggcttcta gtgacgcccc
    12721 gacaatcaac cccgtctgca aatcccccat caacgctcct gctaacggag caacaacaat
    12781 cggacgactc agcatggaca tcccgaacat ttcatgtcca gcaactccaa taaaaacggc
    12841 caaagcgact acaaatgcat catacataat ttttctcctt cctttttcta aaaatcactc
    12901 aaaatagaga tagctgaacg tttttccatc gccggtgtag gctgaaaaac ggtatctgga
    12961 taaagttcaa ctaattcttt taagtcttga atttctgacg tcgttaacat cacttccttt
    13021 gataaggttt tcttatcttc cgatggcggt gtcattttcc cagcattccc cacgttcatt
    13081 aattcgattt ccgttgggaa agctttcgca acttttaccg catctttgac attagggact
    13141 aaaaccatca agcgcatgtc cttcgcccgc ggatctttta acaaatgaat cgcgtcatca
    13201 actgttttaa taatgacttt gacattcgat ggtgcagcca tcttcaatgt catcttttga
    13261 atatcgttgg tagccgcctc atcgcttgct actactaacc cggttaaatt tagactcttt
    13321 gtccaagtca tcgctacttg tccatgaatt aatcgttcat caattcgcac tgctttaatc
    13381 atttttcttc ctcctaaaaa aaatcttcct cttcatttac tacatgtacc tcatcaagaa
    13441 tggtcatctt taattcctgt tggcatttat ttaccagttc ctgaatatcc tcctttgtta
    13501 attccttggt tgccaatact atttcgagca gaattggcag attaaagcca gacaaaatag
    13561 ttacagcaat ctctttctcc aaaaaggttt tggtaatctt ctgattgaca cttccgccaa
    13621 ataaatcggt aaaaacaaaa atctgatcct gctcgttcac ctggtttaca aatacattca
    13681 actcctgttc aaaatcacac tcatccacat aagcattaat caccgtaatc tcttgaacct
    13741 tccccgtcag taattgaata ctgctaagaa agcctgaagc taagtgaccg tgagtcgcta
    13801 ttagaatttt cttcatatgg ttaattcctt tctttctgtt cacttttata taagcaattc
    13861 ctgtgccaac ttttaaaaac attggtttaa tcacgttaat tcacattaaa aaaagtgtct
    13921 aacctaaaaa aaacagatta gacacttttt tagacacttt atttttgaat atacggtcct
    13981 aataattcat agagcagcga aagctcataa gcattgactt taaaattata tttcatctcc
    14041 attgattgaa aaatacttct agaaacataa taaaattcat cttcatctgg tttcaaatca
    14101 taattcggtt cttcagattt ttctgtccga gccaagaata aacgttccat catcaatgcc
    14161 gtatgcatat agagatttag ttttacacgg ccatttaaag tgaaatgata gtaattttca
    14221 tatttagaaa tgactgtttc cacttcactc atcacgatcg ttgggttcag aaagttcaaa
    14281 cggtccgcaa ttccttccaa cgaaaagaat tttactagct cctgaatcaa gcggttaaat
    14341 tctgccggct tcatttcagg actcaaaata tgctccaaat tggctgctcc atcaccgtct
    14401 aagatgtcat aaacattaat attaggaatt gaaaaggaac gtggaagatc cgaagtcgtg
    14461 atgaccaatt ttgtcttgtc aaaataagtt tcatcatttt ggtcgattaa ttcacgtagt
    14521 tctctgtagt ccttcgtgaa aacttcgatg gattcatccg ataaatgatg acgaaaaaca
    14581 tctttcaatt tgtccgaaat ccccaaaccc gacatacagg aaatgataat gttgctactt
    14641 tgagaaaagc cttcaaagta ttgaacattg attgaatact tgtctgctgc atcctccgca
    14701 atctgtttaa atggcgcatt catcgtcatc ttcaagccaa tatcaagtgc aatggaagtc
    14761 gtcaaattat tcagaagaag taactcccct tttaaatcgt tcttgatctg tgtgtaaatt
    14821 tgatttaaag agcccatatc tacgatcagc actagactat ctgtattcac ttgccgtttc
    14881 acgaaattcc ttgtcgattc gacaatctct gacacattac tctctactgg catatccaac
    14941 gcttcaaaaa cataattgcc gcataattga ttcactactg cttgaatgct gctagccgta
    15001 ctatctccgt gagcaagcaa caggcccttc agctcgatgg attcatctac ataatcagat
    15061 aaagtcacga caataagcag tcttagaaag ttctcgtcct tttctgagag cggtggtaaa
    15121 tgctccaaga actgattaca taagtaagtc gtccgaggta gatttgcagc taatgtacga
    15181 tttatcccgg ttaagtctgt cttatagctt ggggaaatga cataggatct gctaataatt
    15241 tctgcaacag gtccactcgt cgtcaatccg tacgtttttc ctaaaacgga tacccagacc
    15301 tttctaaaca tctgaatcat caattttcta ctcattgata aaaaagaatc cttatcaatg
    15361 tgatgcacca aatgctgtac ttcattcttt atcgaagaaa aattttcatt gaaattacct
    15421 tcatttatca gctgagtaat tttttccaaa atcgaatcat aatcaaacgg tttgattcga
    15481 tccgttttta tcttttgatt tttctttatc aataaactag caatcggcaa gctttctact
    15541 tcggattcca gcatatttaa ctgaatcaag atctcatttt tcccaatcgt taatggttct
    15601 tcttgtgttc gactatacac atccgcacaa ctgattttaa tgatattctt cagcttacca
    15661 atattcccac taaagggatg agataacatt aaagacaagg cttctcttga aacaataata
    15721 tctttttgta aaacatccgc ttcattttga aaaaataatt gaatcagctc taatcgttct
    15781 cgaatggggc gatcttcaaa agttggcaac ataacactaa tttgaatacg ccgatcaaag
    15841 gtgtctaata aataattttc acctttttcc gtcgtggcga aaataaaacg aaccttagca
    15901 gaatgccaat ttacattttc accgactgga cgatagtttc cagtatcaat aaatagaaag
    15961 agcttttctt gattttcttt cgataggcga tggacctcat cgagaaaaag atagccgttg
    16021 ttggccgttg ccaataaacc atcggtatcc ttttcagccc ccgtaaaggc ccccttcttg
    16081 tatccaaaca gtgttgccga aagcagttcg ggattgtccg cataatcggc acagtttaag
    16141 atagcaaatg gtgcatcttt ttcaataatt tgctcatgga tagcgtattc aaaaattttt
    16201 gtggctaaaa aactttttcc aacacccgtg gctccattta ttaatacact gagtccgtta
    16261 ggcggatatt tgacagcggc tatacatttt tgaatgacct ctctcagact tccgtcatga
    16321 ccaactacag aatgaaaggc ttcgtttatt tgctccgtat tatctacaac cgcatattca
    16381 tctaaaggct tccacaaaac aggctttccc ggtaatttct cgaccttttt atcttccact
    16441 aatctcgtca aataatggct ggcattctga cgagagatac ctagattctt cgaaattttc
    16501 tcggcagtga cagaagacat attccctgtt agtcgtatca gctcatcaaa aactcgatct
    16561 ttagcgctct ttttcatttt ttcctccagt ttaatgtgtc taaaagcata ataaactatc
    16621 ggctgcaata tgtctattct atatttgtat tccaattttt actttgaaac acaaatagtc
    16681 gaatatttgc ctcagatatc tgacaaaata atttatctac caatttagtt tttatctaat
    16741 atgattttta tgttttttca tagctacaaa tattgaactg gattcatata ttagttttat
    16801 taatagatta cagaagggtt aagtatattt gcatagtttc caattacttt atccttctgt
    16861 aactttttca atcttagatt tactagcccg tctcgggccc tattttacaa tggttttgtc
    16921 cacttttctc tggtagaaaa acgtggtaga aaaaataaaa taacacaaaa aaagcggcgt
    16981 ttcatccgct tttggtagaa gattggaaaa ctcagataga aaaaggacga tttttagctc
    17041 tttagaacgt tgataagtcc ccattcttac tgagttttgc cattatctcc tatacatctt
    17101 gaattctaag aataggtcat tatagatgcc taggtatttc gatcctagat ctttgtagac
    17161 ttcaagatgg gagatcgtgt catcagatgg atagaattgt tcgtccgacg aaatgtcttt
    17221 tggcaataac gcttttgctt cttcatttgg tgtagaataa ccaatatact ctgcattttg
    17281 tgccgcattt tccggttcta gcataaagtt gatgaaatca taggcacctt ctttattttt
    17341 cacggttttt ggcataacga tattatcaaa ccaaagattg gatccttctg gtgggatcac
    17401 ataatgtaaa tgttcatttt cccacatcat atcggcagct tcgccagaaa atgtcactgc
    17461 cacggaggct tcttcattga tcatatacat tttgatctca tctgccacga ttgctttgac
    17521 atttggtgtt aaatgattca atttatttgc tgcttctgtc agctgtgcca tattcttact
    17581 attcaaagaa tagcctaaac tattcaaaga aagccccata acttctcgtg caccgtcaat
    17641 cagcatcaaa ctgtctttta attccggctt ccacaaatca tcccaatgct taatttgatt
    17701 tgcatcaaag aatttatcgt tatatacgat ccctaaagtt ccccaaaaat atggaatcga
    17761 atactgatta tctggatcaa aatccaagtc taaaaatcgt ggatcaatat tatttaatcc
    17821 tttgatcttc gaatgatcta atggaagtac tagtttttct ttgatcattt tttggatcat
    17881 atattcactt ggtatagcga tgtcgtagtt tgtcccacct tgctggattt ttgtgaacat
    17941 cgcttcatta gaatcaaatg tttcataatt cactttgtat ccatattctt tttcaaattt
    18001 cgtgatcaaa ctaggatcga tataatctcc ccagttatag atggtaagta cctttgctcc
    18061 actcatgcca cttgtttttt caagttgacg aacaccaagc agcagcaccg caataatgat
    18121 gagaatacct aaaaacagag attgtaattt tttcatcgca gccgtgccac cccctcgcgt
    18181 actcttctgt ttttcttcag ccgttttgga cggttttctt tactaataaa gtagtatcca
    18241 atgaccaata tcatggagaa caagaaaact aacgcactta gcgcattgat ttctaaactg
    18301 attccttgtc ttgcacgaga atagatctct actgagagcg tcgtaaagcc atttcctgtg
    18361 acgaaaaatg taaccgcaaa atcatcaaga gaataggtaa atgccataaa atatccagct
    18421 atgattcctg gagaaagata aggcaaaatg atatttttga ctacttggaa attattagca
    18481 ccaaggtctc tagccgcatc gaccattgaa tcgttcattt cctgcaattt tggcaagacc
    18541 atcaagacga cgattggaat gctgaaagca atatgggaaa gcaatacact ctgaaaacct
    18601 aatccgaatc ctagtagtgt aaagaaaatc aagaaactag caccgataat aacatcagga
    18661 gagactaata agatgttatt catgctcaac aacgctgtcc gtgcacgtcg ttttttcgtg
    18721 taatagattc ccatagcccc aaatgtcccg atgatcgtgg cgatcaatgc agataagaaa
    18781 gccagcatga acgtattcaa tacgataatg atcaaacgtg tatcttcaaa aacagcttca
    18841 taattttccc aagtaaatcc tgtaaagctg ttcatcgttc ctccttcatt gaaggaataa
    18901 aaaatcagat agaagatcgg tgcatataag aataaaaaga cgatccctaa ataaagatac
    18961 gaccatttaa attttttcat ttatgagcga cctccttttt tcttctcgcc tgtcaggagc
    19021 atcacgatga acatagcaat gattaaaatc acaccgattg tagaacccat cccccagttt
    19081 tgtgtaacca agaaatgctg ttcgatagcc gttcctaaag tgatcacccg atttccgcca
    19141 atcagacgag tcagcatgaa taaagaaaga gaaggaataa atactgcttg gacaccactt
    19201 ttcacaccat ttaatgataa cggaaaaatc actcgtcgga aggtctcaaa gctattcgcg
    19261 cctaaatctc tactagcact gatcaaagaa ggattcatct cttccaatgc attgaaaatt
    19321 ggcataatca taaatggaat ctcgatatac gttgccacga acagaaaact gaagtctgtg
    19381 aataatatct gtttcggccc gatcccgata aaatcaaaga aatgattcac gctgccgtgc
    19441 acactgaaaa tcccaataaa tgcgtaagct ttaagcagca gattcaccca tgtaggcaaa
    19501 ataatcagca tcagccataa ttgcttgtgc ttcagttttg ttaaaaaata agcagtcggg
    19561 taactgatga tcagcgtgaa aaacgtgatc aaaaatgcat accatacaga attgatcgtc
    19621 atagaaagcg atgtccccga cgtaaaataa gatagataat tattcagcgt gaattgccca
    19681 ttcatatcaa aaaaagattg ataaatgatc attaatacag gcgcgatcac gaataaaccg
    19741 agccacaaaa tataagggac ggaatagatc ctgcgcattg ttttcattct tgtcccccct
    19801 attcttcgta gctgtcaaga cgtgcatcga attcttcttc agactcgttg aaacgcatga
    19861 catggatatc ttctggctca aaggccaatc ctactttgct tccttcttta gcttttttcg
    19921 ttgaatggac catccattca ttttgctgtt catcataaca gatgatttca taatggacac
    19981 cacgaaacag ctgagtatcg accgttacga ctagttttcc tttatctggt gtcgtgatcg
    20041 tcaaatcctc tgggcgtaaa acaacttcaa ctggttcatt tagacgcatc ccgccatcga
    20101 cacattcaaa ttgcttacca acaaactcaa ctagattatc ttcgatcatt acaccgtcca
    20161 cgatattgct ctccccaaca aaatcagcaa cgaaatgatt gattggctca tcataaatat
    20221 ctacgggtgt accactttgg acgattttcc ctttattcat gacaaaaatc tcatcactca
    20281 ttgccaacgc ttcttcttgg tcatgagtaa caaaaataaa cgtgattcct aatcgttgtt
    20341 gcagatcccg cagttcatac tgcatatctg tacgtagttt caaatcaagc gccgataacg
    20401 gttcatctaa tagcaatact ttcggttcat ttacaattgc tcgtgcaatc gctacacgct
    20461 gtctttgacc accagacatt tcactaatct cccgtttttc gtatccagga agctggacca
    20521 tacgcaaagc atcttttacc ttcttttcga tatctgattt ggacattttc ttgatcttca
    20581 atccaaaagc aacgttatca aaaacattca tatgaggaaa aagtgcataa tcttggaaga
    20641 ctgtatttac ttgtcgctta ttagctggca aatcattcaa ccgtttccct tcaaaataga
    20701 tgtccccttc tgtagcttcg gtgaatccag ctataatacg taggatcgtt gttttaccac
    20761 agccggaagg accaagcagt gtataaaatt ttccttgttc gatctcaaaa cttacttttt
    20821 ttaatactgt ttcatcatca tagcgtttca ctacttgatc aaatgaaatg attggtttcc
    20881 ccacgttttt accccctata ttacaaatat gattgcgttg caacaatcaa tacttttgtt
    20941 cgttttttga gatgattcct taattgatgt tgttttgtcg cttgataata gatcgactgt
    21001 cctgcttttg ccacatagac cgtctctcct aagcttaact ctaattcccc ttctaataca
    21061 aaaataaacg tttcagataa tgaaggttca aacgtcttat attctcctgc accttcaaaa
    21121 gtcagcaaga caggctccat ttctttttca ttcgaatcag ccacgagcca ttccaattca
    21181 tacccgtttt cttcatcgaa ataaaccgta tgatcttctt ttgaataaac cacttgcaga
    21241 ttcgctgctt cttgatgaaa aaattcttct ggagtcacac ctaagacttc aagaatagaa
    21301 aagaatgttt ccattgatgg tgaacttaaa tctcgttcca actgggaaat ataccctttt
    21361 gacaaatctg tgcgttcgcc tagttcttct tgtgtcaaat tcttttggat gcgcaaattt
    21421 cttaacttct caccaatttc cataatttcc tccgaatcga agttttctaa tagtaaactt
    21481 cctgtttact accactcgta ttagtatacg aaatttattg aaaaaggcaa ccctttgtaa
    21541 aatttttttt gctgaatatt tctttatctt ttggaataaa ttctagttgt ttgattcttt
    21601 atttttcacc taatttttgt ctaaatttac ggaagaacaa gaataaaaga gtaggcttta
    21661 tggtatgata aaagaaaatt ttaaaaggag tgtctcacga atgaacgctt ttcgatcttt
    21721 tcaggtaata aaggaacctg ttttccatac agcaatcagt gatctgccat ttactgagtt
    21781 ggctgaaaat gaagtcttgg taaaaattgc ctattctgat gtcaattata aagatgcatt
    21841 ggcaatgagc gagtctggtc aggtgatacg aacatatccg atgactccag ggattgattt
    21901 atccggaact gtcgtccaaa gtaacaatcc acgtttttca aaagaagatc ttgtacttgc
    21961 tactggtttt gggcttggcg tcacccatcc aggcggttat agccaatatc aaaaagtacc
    22021 aggagactgg ttagttcctc taccaaaaaa tatgacgctg cgtcaagcga tgatccttgg
    22081 tacagcaggg tttactgcta tgttgtgtgt gaacgcttta atccgtcaag gtatgacctc
    22141 cgataaaaaa gttgtcgtta ctggtgcttc tggcggtgta ggcagtacag cgatagccct
    22201 gttacataaa ctaggattcc agtcaatcat tgccttttcc agaaaagaag agtctgtcac
    22261 atggctaaaa agtctaggtg ctttgcaagt cgtacgacct gatgaatttc tgccagagac
    22321 aacaaaagct ttaggaaaac agcaaatcga ctatgtcatc gacacagtag gtggcgagca
    22381 gctaagcagt cttcttccat tgatctctta taacggtgca gtagctttat gtggcaatgc
    22441 tgggggaatc aaactaaacg cgactgtctt gccttttatt ctgcgaaaca tccagttgat
    22501 tggaattgat tctgtcaatg taccaataga tcaacgtctt tctttatggc aacagatggc
    22561 ggatttgcag attgcagatg aactggtcgt ccaagagatt actctggatc aattgcctga
    22621 aacagcatct aaactacttg ctggaacaca ccaaggaaga acactagtaa atgtaggtga
    22681 ccataaatga agattctaat cacagctgga ggaacgactg aaaaaatcga tcaagtacgt
    22741 gctattacaa atcattcgac aggacgatta gggcaagcat tagctgatta tttagctgcc
    22801 aatccggata ccacaatcga ttacgtgact acgaaacaag cactgaaacc gaaaagacac
    22861 tccaatataa cgatttatac gatcgaatct gcactggact tatttttaca attagaagaa
    22921 ctaacgaaaa aagaacacta cgatgcgatt atccacagta tggcagtcag tgattttact
    22981 cctgctttta gtttttcaga agaacagctg gcaaaaaacc tacccacttc ttcaacgcaa
    23041 gaagaactgg acaattggtt tgcggaaaat gaacagacag ataccactgt ttcaaagatc
    23101 tcttctaaca ccgagcatct tgtgctcgtt ttgaagaaaa caccaaaaat catttcttat
    23161 ctacgtgaat ggcagccaaa agcaaaaatc attggtttca aactattagt agatgtacca
    23221 aaggaatctc ttctggcagt cgcaaaaaat agtctgataa ataacaaaac tgattttatt
    23281 ttcgcaaatg atttgacgga aatccatggc gagacacacc acggctatct cttatctaaa
    23341 gatggaacag tggaagaagc tcaaagtaaa tctgagattg ctgcattgat tactgagaaa
    23401 attagactgg aggaatcaaa atgaaaaaga tcttactagg cgttactgga agtatttctg
    23461 cttacaaaag cgctgatctt acaagtcaat tcgtcaaatt aggtgcccag gtagatgtga
    23521 ttatgtctaa aagcagcaca cattttatca cgccattaac cttgcaatct ctgtcgaagc
    23581 gcgcagtcca tacagacgtc atgcaagaat caaatccgca ggtaatcaat catatcgaac
    23641 tagcaaaaca agctgacttg tttctcatcg cgcctgctac agcaaacatt atcggcaagc
    23701 tagcaaacgg cattgcagac gatcttcttt caacagtcgc aatggcgctt ccagtgggaa
    23761 caccaaaatt gattgcccct gccatgaaca cgaatatgta tcaaaacccg atcactcagc
    23821 gtaatctgga aacattgaga tctgtcggct atcaggaaat tgaaccaaga gaatctcttt
    23881 tagcttgtgg agattttgga aaaggtgcct tagctgatat tcagacgatc atcgaacagg
    23941 tcatgctgac tcttaatgaa agaaatgctt agaaaaaagg agtacttatg aaaaatacaa
    24001 aaaactttac attaactgcg atgtttttag ctattttgat tttattagct gttacgcctt
    24061 taggttttat ccctatcggt ccaatcaatg caacaaccat gcatattcct gtaatcattg
    24121 cttcgatcgt tttaggacca agactaggtg cttttcttgg cggtacattt ggactgatca
    24181 gcatgatccg tagcacagtt atccaaacac ctttgtcttt tgtcttttct ccgtttatcc
    24241 cagtcatcgg aacagaacat ggaagctgga aagccttgct gatcgcgttt attcctcgca
    24301 tcttgattgg ggtggttcct tatttcgttt ataaaggtct gcgcaaacta atgaaacaaa
    24361 aagcagattc cgtttcttta ttcttagctg gactttccgg ttcattgacc aatacgattt
    24421 tagtgatgaa tatgatctac tttttattcc agcaagatta tgccaaagtc atcggaacaa
    24481 atttaaatgc tgtttattca gctattctag cggttatttt tacaagtggt atacctgaag
    24541 gaatcgtcgc aggtcttgcg actgcagcag tatgcaatgt cttgttaaga tacttcaagc
    24601 acccttctct tactaactga tagtcatcgt agccgatctc tgattttttt ctcttttggt
    24661 gttactattg aatggaaaga tttgacgtcg aaaggatgat ccaaatgaaa atttttgttg
    24721 taggtgcaaa cggacaaatt ggccgacacc tgataaaaga tttagctcca agttcccatg
    24781 aaatttttgc aggggtgaga gatgttgctt cccaaacatt agtaaaaaaa gagaatgttt
    24841 cgtatgtctc ttttgattta acttggtccg tggaaaaaat gaccgaagca ttcaaaggca
    24901 tcgatgtatt gatttttgct gccggctctc aaggaaagaa tcttctgcaa gtagacttgg
    24961 atggcgcaat caaaacagtg atcgcagctg aaaatgcgca tgtctctcgc tatctgatgg
    25021 tcagtgctgt ttatgcagat gaaccagcaa aatggcctga gtccatgaca gattattata
    25081 ttactaaaca ttatgcagat gaatggctga aacgaactaa cttagatttt gtcattcttc
    25141 aaccagtcac attgactaac gatgaagaag ttacttccat tcaattaact aagcctaatg
    25201 agaaggcatc aaaaacgatc actcgctcta cagttgctgc tgtacttgcc gctctagtgg
    25261 aagaaacgga tatttctcgt acgactctag tactttctga aggtagcaaa gagttgaata
    25321 cagcattcca agaatgggca aaggaggaat agatccatgc gtgccatttc aatcaagcat
    25381 cctggttctg tagatgtatt agaacaaatt gaagtgcctg ttcctaaacc aaaacaaggc
    25441 gaattactga tcaaagtcca tacagcagct gtcaatcgaa cagatattat gaaaagagaa
    25501 tcagtctctt tgaaacctcc ctaccctatt ttgggagtcg aagtagctgg agaggtagtt
    25561 gagaataaaa gtgatatctc cacttttact cctgggacaa aagtatacgg attagtcaat
    25621 ctaggtggtt atgccgaata tgctgtgatg cctgccgatc gtgcaatcct tctccctgat
    25681 acgctagact atgtttctgc tgctggaatc tcagaagttt ttttgactgc ttatcaaaca
    25741 ctttactggc ttggaaaatt acaagaaggc gaaactgtct tgatccatgc aggagcaagt
    25801 ggtgtgggaa cagcagcgat ccaattagct agacagcttt ctcacgcgaa aatcatcgtt
    25861 actgcaggat cagaagaaaa actcgccttt tgtaaagaac ttggtgcaga tgaattgatc
    25921 aactataaaa aacaagactt tcaagaagag gtacgaaaaa tcactgaagg tcggggagtc
    25981 gatgttattt tggatttcat cggcgcttct tactgggaaa agaatttagc ttctatagcc
    26041 gttgatggac gttgggtctt gattggtatg ctaggtggga ccatcgtgcc tgagatagac
    26101 cttggtgtat tgattgggaa acgtgtccaa ctgcttggta cattattgac acctagaagt
    26161 gatcagtata aagcagagct cgcacaaact tttatgcaaa aagctgggcc ttttctagaa
    26221 aatggcaaga ttcggccaat cattgaccgt acattccaat tcagcgcagt caaacaagcg
    26281 catgaatata tggaagcaaa caaaaacatc ggaaaaatca ttttgaagat aactgaataa
    26341 aatcatgctg atcaaaaaag aggctgggac agaagcgttt aactccaaga aataagaaga
    26401 aattcacgaa aattgctttt caaatttttg tgaatttcag cttatttccg aaggaatttc
    26461 ttctgcttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct ttacttttgt
    26521 cccacgctct ttttccgaat aaacggtgtt caaaagcttg catctgcggt aataagtcca
    26581 accaagttaa aaacataaga agctgttttt acttggctgt tcttattact tgatgcaggg
    26641 cagctttttc acaacctctt cactaaacag tgttcaatca gctgaccctc ggtattagct
    26701 caaaacgctg atttcacaac ctctttttat ttacgttcta tttcattcat tttccgaagc
    26761 aaatcgagct gcttgtatgc ccgcttgtcg cccaaaaata atgatctccg caactgaatt
    26821 tccaccgatc cggttgtttc catgtaagcc acctgtgact tcaccagcag catataaccc
    26881 cctaattggc tggttttctt tatctaatac ttcagtgttc gtattgattt tcacaccgcc
    26941 catcgtgtaa tggattcctg gtgcgatttt gatcgcataa taattcggag acgataagtc
    27001 atgttccatg gctgtggcac ggttgaattg agcatctttt ttctccgcaa catccgtatt
    27061 ccatccatca actgtttttg ctagtgtttc ttttggtaaa tcgatttttt ctgctaattc
    27121 ttccattgtc tttccagttg tgacatatcc tttttggtca taaaattcaa tagctgtggc
    27181 acgatcacgc acgccttggt caaaaatcaa ataagctgat ttttcaggca atgctgtaat
    27241 tgctgcagat actttatccc ttgtatccat ttcattgaca aatcttttcc cctcttgatc
    27301 gaccaagatt gctccttctc cgcgaacaac ctcaccaatc aagaccccct catcctgctg
    27361 cacagttgga tgaatttgta ttttatccat atcaacggtt tgtccaccta atttttcaat
    27421 catggtgata ccatctccag ttgatccttc ttgatttgtc gtaatataat cagctaaatc
    27481 gggacgatat ttttcaataa attctttgct ggcaccaaaa ccgcctgtgg taactacgat
    27541 tgcttttccg gtgacctctt ttggttcttc tccttgaaca tgtacagtca cttcattgac
    27601 cgtttcccca tttttcttaa tgtctgtcac atctgcgttg acaaaaatag ggatttcccg
    27661 ctcatggacg ttattcaata acccctccac taagtatcca ccaatagccg agccatcaga
    27721 cggacgatgt gtcctctttt cactcatccc tccagtgatc gttaagttgt ccagtttgat
    27781 accgttttga tctagccaat cgaccgcatc agcagagtga tcgacaaagt atcgaagcat
    27841 ttctttatca ttcgttcctc caccaccttt tagcgtttct tcatagaagg catcattcga
    27901 gtctttgatt ccttcttctt tttgaaattt tgtttctgaa gcattcatac cgcttgatga
    27961 tttagatgtg tttcctccgg caacaggcat cttttccaaa attactggat tcaacccagc
    28021 atcttttgct tcgatagctg ctgccatccc tgcaccacca gctccaataa tcacgacatc
    28081 ataagattct ttcatttctt ttggatctgt atatacttgt tcagaagcgc ctgatgatac
    28141 ttcggcagat tccttcgtac tagaacttgt ctgcgctgta tttttctgat ctgcgccgca
    28201 tgcgctcata gcaagaagtg caacggctgt taataaacca gcaactaatt ttttcatttg
    28261 tatccccctt ttctccttat actatattaa gtgatttttt tcacaatatc aactctgttt
    28321 ttctttaagt catatagctt tggtaaacac acgcttctta gtataattaa ggatatacat
    28381 gaaggagtta ttttatgaat catttgaaaa ccttttttac gaacagtaca aaaactgcag
    28441 gtagaatcac aaacggaatc tatattttat tacgtatcat cgtcgttatt atgctagtgg
    28501 atcaggtctg gaaccaacac tggggaaata caatgctttt attactgaca ttaattttat
    28561 ttgaaatccc ttattttctg gaaaagatat taaagataca gattccaaat atgctagaaa
    28621 tcattttgtt atcttttatt tttagttcaa cagttttagg agagttaagt gatttttatg
    28681 gctatttttc aatgtgggat acttttctac atgcattcag cggattttta gctgggggtg
    28741 tcggcttttc ccttgtttat ttgctcaata aaaatacaaa aaccatgaac ttgactcctt
    28801 tattactagc agttgtttcc ttctgttttt cgatgacagt tggtgttatg tgggagtttg
    28861 tggaattttc agcagataaa ttactgcaat tcgatatgca aaaagatacg atcatccagc
    28921 aaatcaatag tgtggagtta agtaaagacg gaaacactgt ctatcatgtt ccagatatca
    28981 ctcggacaat cattgaaagt caaaatgcag atggtaaaac agataaaaca atcatccaaa
    29041 atggttactt agacgtggga atcatggata ctatgaagga tttatttgtt aattttattg
    29101 gagcagttgt tttcagtgta tttggttacc tctatagtcg aaacgattac caaaaattta
    29161 cgttcatcaa gaattttatt cctaaaaaaa tcaaacgata aaacatgcta aaaaacaaga
    29221 gaaggggcag tgacaatact tttgtcactg ccccttcttt tttgaatgta tggcatgcca
    29281 ttagttatcc gatagtatta actcaaaaaa gtaagatatg aggaatgact atttcgtttt
    29341 ttatcccaat actgaggact tatttcacca tttcgttatt tatctaattt acttaaaacc
    29401 tgcataactt tttcataatc tggattaggt gtgccgtcta gattttcagc aataaaagta
    29461 aaacccattt ttttataaag agcaatcgcc tgatggctcc atgtctgtgt gtgaagatag
    29521 attggtccgt ccgtatggag ggtaggaaat tcttgcaaga ctctggcagt caatgctttt
    29581 gcgagccctt ttccttggta atctggtttc actgctaacc aatgaaaaac cggatacact
    29641 tgctctcttg atttttttcg ccaagcggta caggttgcta ctttttctcc tttttttgtc
    29701 tctacaaaaa acatccttct ttcaagttct tcaggataag gagcaaagtt ttgttgaaaa
    29761 tactcaagtg cttcatctgt cgtttgaaat tccccaacgg aagtctcaat ctccgcccaa
    29821 gcagattcat cttctttttg ataaaatcga aattcaaaac cttccgctaa aggcgtgatt
    29881 ggaacatttt gttgaagtgg tcttgtcatc cagatttctg cgtaaggtaa cgttttatct
    29941 aacatttttc tatccctcct cgtctccatt atgccaaata acatatgact cagctactta
    30001 tttgactata acagatagag atataaatca gaaccagtca tctgcgtcaa attcatttcg
    30061 attagcgtat acccatgttc ctgccatacc tcgcatgaaa tctcctaaag cagaagaagg
    30121 aatacctcca ccaggtgcgg gtgttcccgg actaggaagt ggtacttgcg gctgcagatg
    30181 ctcatcggtg gaatcaatag ttgacaagcg ttttttcttc gtttccttct ggtcatctga
    30241 ttccttctga tcttccgact gtttagcaac aaattctttt tctgattgtt gttttatttt
    30301 aggctctaaa atttcatttt ccaatccaga aacttgtggg gccaaatgag cttcttgcct
    30361 tatcgtttta ttagattctg agatttttct agatattctt gctaactcta ggcttcttgt
    30421 ttgttttact ttccctgttg gagaaactgc atgagccatt tgttgttttt tttctgacaa
    30481 gctttcttct tgtctttttt gttctttttg atctatctgt ttgtttaatg gtaaaacatg
    30541 taatagtgac tgagcatgga gttcttggag tgaatacggg tcatgataac tatacataat
    30601 cgtaaaatca cctgtttgtt tggtcaaata accatcctcc aatggtactt cattgattgt
    30661 aaccgataca tcctttaacg gtacgggttt acctaaggaa tttgttgctg attggatcaa
    30721 ctcttctttg tttatttttt cgtttggatg aacaataaga tcatgacctt ctaatgaacg
    30781 attgtctggt atataaacat aagtaaaggt aatttcccct cctgattgct tcctattatt
    30841 catcagttct gagtgaagta cttcaaggat ctccgaccag tgactttttc tgtctaatcc
    30901 gtttgcttgc atccaatcag aaatagaagt gactttccca ttttgttcat aagtcgataa
    30961 atctatattt ggcactcgat cagaaaattc agcaggtagg cgcaaatcag gaatacgatt
    31021 tacactactc atatgtgttt ctgcttgaag ttctttattt gtttgactat ctataaaatg
    31081 agtgatgaca gtcgatgaag cagcagatgc ttctacataa actacagctg ttgtttctgc
    31141 cacatttgat gttttcgagt ctatataagt taatacaacg ggatattctc ccggagttgt
    31201 agtatctact gtcccgttca ttttgatttc ttctttattt cctgaagttc catcgctttt
    31261 tgttacatat tcaacaagat ctttaggggc gtaactctct cctgttctta agtgaagatc
    31321 ttttcctttg atagctgttt gatcagcacg aacatggatc acagcagatg cagtcattgt
    31381 ttttaaagca attggatcaa aataggacaa ggaaatagta gatgttcctg tattagtcgt
    31441 atccacgata ttcccattgt ttgaaattac tttggacata tctccttgtg ttccatctgc
    31501 gttcttcagc ctttctacca gatcagatat cgaaactttt gtccctgcca gaacatcgat
    31561 tttctttctt tcaaactgtg aataatcttt ttcatataca taagaaactt gtgcgcttcc
    31621 accgtccttt atatctgtca aagcatgatt caaaatatca gtgatctgtt ttaactgatc
    31681 accttttgta ggatcagtca tatcggaaaa gatagaaagc ggcacactgc ttccggtttc
    31741 cgatgtccat aatgttttgt taggaattaa ccagtagtgg tttatcgttg gaggataact
    31801 gactttatta taggaagtca atgcaggaag tagctgtttt ccatttgcat cagtgaaagt
    31861 atgagtaata gtgatagggg aatcagtggt ctcagaaaca gaggtacgta cgaccttgat
    31921 ggtttgtgtc gttgcctgaa cgggtacatg catatagaca cctacagata aactgatcag
    31981 catcgtgcgc atcataaact taacgtagtg tttttcagaa cacgaccgtt ccttatctaa
    32041 acgacaagcg tgtttattta gttgttttga acgtcttttt gaacacattt attcttagtc
    32101 ctccctactc ttgtctaaac atgaagtagt aattttcata agatgatttc attatataaa
    32161 taaaatcaaa agaagatgga ctattaggag agtatttttt ttgatttttt attgtttcaa
    32221 gaaaatcgga ataataactt atgtactttt taattttgtt aagtaatata tctagttata
    32281 tctagttata tctagttacc ttttctttga aaaaaacata aatcagctaa taaatatgtg
    32341 agacaaagct gccatccata aatactagct gattttttca gttaaatgtc tatttttttc
    32401 tctgatgatg gtatttccaa aatattccca gtcatctgga gtcggtggat ttttccaaat
    32461 tatctgttct ttattaaatt tttcaagtaa acaatcagat aaataaatat tcgtacgaga
    32521 agtgattcga ctctcaatca caatattgta atttttaaat cctttcactt gagaaataat
    32581 gtaatcggta taagcagatc cttgcgaaaa atcaatacag acatgaatcg gtgcttcaaa
    32641 atatgatacc ggacaaatac cgaccaataa aaagagataa tcataaaata agcgaatcat
    32701 gaaacgttca tcttttccca agaaatagcg tttattctct tgtatgacct tttggattgc
    32761 tgtactgaat attggatacg tctcgaagaa aaactgtagc tgctttttag acgtaaagct
    32821 agtcatctca aagtcgaata tccaattttt tcgattgaca tgcatcatac ccatacgaag
    32881 ttcttttcgt atatatagtc tttcttcttc agagatttca atcgaaaatg gtaattgatc
    32941 aatcaattcc actgacattt tttgactaat catgtggata tcatgaaaat gagcttccgc
    33001 aagtccaaat gcgccatcta attcaaattc ttcttccgtg atcaaaaaca acatgatata
    33061 agaaatctca tcaatgatct gctctttagg gatatgaaga aaagaaatat gtgataaatc
    33121 cttgatgatc ttcattcctt ctgagctctt aaagtattca tctgaaaaga aacgttttcc
    33181 gattggttga ttcccatagg ccctcaacaa actaatagtg gttagcaaat tgaatttgat
    33241 tcgttttgtt gcagacagtt ttaaattgaa cagatattca aaatattgca cgatactctt
    33301 tgcttcattc atgacgcttt ctctaaatgg catagaaaaa ccattaaaga ccccgaaata
    33361 taccccaaat aatacgttcc gtatgtttat ttcttcccca actaatatgt ttttctttaa
    33421 ctttatttga tgctgttcaa gaaaagagac taattctctt ctttttacat aggctcttga
    33481 tatgcttaaa aaatgattct ctgcaaattt ttcgatcgtt acttttgaat ccacgatctc
    33541 cgttaacagt cgagcaattt ttgatcgttg aaagtagatt cttcttaaat ttttgatatc
    33601 cgtcaaattc agacgttcga catggatctc tgcttcttct gaaatttgga tctctggctt
    33661 tgttttgaat tcagtaaatt ctttatttaa ttctatgata taatttcttg ttttaaattt
    33721 agacaaacct aatgtttcat ataacttagg cagcactaca taattctttt tctcaaattc
    33781 taaatatcta gctagcagca gtttttctct atcttcctct tctataaggt aatccaactt
    33841 catatcctac acctcaaaaa atgcctcata ccaattactt gatatgcaga tgaaagttca
    33901 tcttttcacc tatactgatt ttatgatgga ttatgcttgt tttttcatca gttattctca
    33961 ttttttatct tttttttgag aatattgcaa aaaatggaaa aatttatgag gaaatattct
    34021 atattttttg ttttattgtg ttaaaatgta caagatttgc gccatttgcg taaacaacca
    34081 caaaaaagag caggacggcc accgtcctgc tcttttactt attttaagtt ccattactta
    34141 ttaattggct aaacttctgc ttgtttctga tattgcttgt ttttctccaa tgatccgcac
    34201 ttctgtttct aaagacacat caaatttttc tttgattact tcttggatgt gcgcaatcaa
    34261 ttcaatataa tctgtcgctg tcgcatgatc gatattgacg atgaatccag catgtttttc
    34321 agagacttgt gcaccgcccc atttcaggcc ttgcaatcct gcttgttgga tcagctgacc
    34381 tgtatagtgt cctactgggc gcttaaagac acttccgcat gaagggtatt ctaatggctg
    34441 ttttgattga cgcaattcgg tcaactcatc cattcttgct ttgattgctt cataatcccc
    34501 tgtttgcaaa ctgaatcgag cttccaatac gatagcacgt aattcttgga ctttactgtg
    34561 acgataagaa aataccattt cgtcttttgt caaagttttc aatgttccat cttcaagcaa
    34621 aagatccact tcagcaaaaa catctttgat ctctccgcca tacgcacctg cattcatata
    34681 aaccgctcca ccgacacttc ccgggatgcc gcaagcaaat tcaaatccag tcaatgattc
    34741 tgccaaagct gcataagtcg tatcaatcag tgaggcacct gcttccgcaa tgaccatcgt
    34801 tcctttgaca tgaatctgtt tcatttcagt cagcatgatg actacaccgc gaatcccgcc
    34861 atcttgaacg atcagattgc ttgcatttcc taataccatc caaggtatat catggattcg
    34921 gcaataatcg atgatttgct ttacttcttc ttgttttttc gggaatgcaa ggacatcagc
    34981 tggtccacct gttttcgtaa acgtatagtt catcaatggt tcatcaaaca atatagtaat
    35041 cgtggctaag ctttcaacta tttcttcttt attcactgag gattttcctt tcttttctca
    35101 aacgtcctca ttattctatc acgcttaata caatcttgcc agtcttgttc aaagttgatg
    35161 acggtagtga tctttaagca cttcctcaca taattcatga cttcttacaa cttctttttg
    35221 tttaagcaat tctgtttccg atttacctaa acggatctga gaaaagaagg cctctaccat
    35281 tggctcaaac ccgcgtttga ctagcgtgtt tgtccagtcg ccaaattctt tcactttttt
    35341 tccatcagca tcttgaatga caagctgggt cagatcgttt acttcataag taccattttt
    35401 acttgttact tgatattttt ctatattagc gccacttgct aaatccatcg ttaaaatagc
    35461 cgtttgattt tgtgtttcta attgtaaaat ggcatattcc aaaaattcct ttgtttctct
    35521 tatctggtat tttgttctca aaatgggttc atctaataaa tacacagctg tatcaataag
    35581 atgtaaaaac aaatcataaa ggacgaactc tgtcgtttct tttgccgcta ttcgattttt
    35641 ttgcaggtgg atcaaccgct tatctgggat acgtttcaat tcttctacca taggagcaaa
    35701 gcgccggttg aaaccgatca tcaaaattat ttggttctta tcagctaatt cttgcaattc
    35761 acgaacttca tttagatgga cactcagtgg tttgtcaata taaacatgga taccattttc
    35821 tagacatttt ttggctaatt cataatgtac ttttgttgct gcgtggatca tacatgcttc
    35881 gattttttct tctaaaagct catcgatcgt ttcatataga tgttggaacc cgtactcttt
    35941 ttgaattctt tcttttgttt ctttatttct tgttgcaaaa taaaaatcaa gtgttccttg
    36001 ttttttcgca taagttggta aatatgcttt ttctgcgata ttccctacac cgattacacc
    36061 aatcttcatc tactttcctc ctggatcatt tagttacttt catcttacac cattttttac
    36121 taaaatgagg tacaatagta tcgcaaaggt aaggaaatta attcgaagga gtgtgctttt
    36181 attgaaattt atctcttgga acgtaaacgg gcttcgtgcc atcgtaaata aaaatttttt
    36241 agaagttttt catgagttag acgccgattt tttctgtctg caggaaacga aactccaagc
    36301 aggacaaatt gatttagatc ttccaggtta ttatcaatat tggaattacg cggaacgaaa
    36361 aggctattcc ggcacagcga tttttgctaa aaaaccagca ttgaatgcca cttacggaat
    36421 gggtatcgat atccatgata cagaaggccg tctgattact ttggaatatt ccgatttctt
    36481 tcttgtaacc tgttacacgc caaattctca aagcgaatta aaacgtttag attaccgatt
    36541 agaatgggaa gaagcctttt ataattattt ggaaaatcta aaaaaacaaa aaccagtcat
    36601 cgtgtgtggg gatctaaatg tcgctcatca aaagattgat ttgaaaaact ggaaaacgaa
    36661 tcaaaaaaat gctggattca ctccagaaga aagagcggct ctttctcgac tgttggataa
    36721 cgggtttatc gatacattcc gttattttta tccaacccag gaaggtgtct actcttggtg
    36781 gaactaccga ttcaattcaa ggaaaaacaa tgctggttgg cggatcgatt actttttgac
    36841 gagcaaagat ttagagccag gactagctga tgcaaagatc catacggaca ttatgggcag
    36901 tgatcattgt ccagtagaat tagatttgaa ataaagaggt tgtgaaaaag tcgtttagtt
    36961 tcgagtatta agaaaaattt tgtgaaaatg gctttccaca ttttgcataa atttggcata
    37021 ataccgagaa actggctttt gaacactgtt tatcgagaga ttgtgaaaaa gctgtcctgc
    37081 atcaagtaat aagaacagcc aaacaaaaat agttcctcat atttttcgtt tggttgggct
    37141 tat
//
LOCUS       DAMEET010000016        37004 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.016, whole genome shotgun sequence.
ACCESSION   DAMEET010000016 DAMEET010000000
VERSION     DAMEET010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 37004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.016"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            299..1960
                     /locus_tag="PB780_000712"
     CDS             299..1960
                     /locus_tag="PB780_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDE0732783.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2030..2377)
                     /locus_tag="PB780_000713"
     CDS             complement(2030..2377)
                     /locus_tag="PB780_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HDE0732784.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2461..3402
                     /locus_tag="PB780_000714"
     CDS             2461..3402
                     /locus_tag="PB780_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDE0732785.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3403..4446
                     /locus_tag="PB780_000715"
     CDS             3403..4446
                     /locus_tag="PB780_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDE0732786.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4452..5522
                     /locus_tag="PB780_000716"
     CDS             4452..5522
                     /locus_tag="PB780_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDE0732787.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5512..6465
                     /locus_tag="PB780_000717"
     CDS             5512..6465
                     /locus_tag="PB780_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDE0732788.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6477..7487
                     /locus_tag="PB780_000718"
     CDS             6477..7487
                     /locus_tag="PB780_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDE0732789.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7772..8593)
                     /locus_tag="PB780_000719"
     CDS             complement(7772..8593)
                     /locus_tag="PB780_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDE0732790.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8690..8968)
                     /locus_tag="PB780_000720"
     CDS             complement(8690..8968)
                     /locus_tag="PB780_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732791.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9236..11245
                     /gene="metG"
                     /locus_tag="PB780_000721"
     CDS             9236..11245
                     /gene="metG"
                     /locus_tag="PB780_000721"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDE0732792.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11724..12311)
                     /locus_tag="PB780_000722"
     CDS             complement(11724..12311)
                     /locus_tag="PB780_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732793.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12486..13808
                     /locus_tag="PB780_000723"
     CDS             12486..13808
                     /locus_tag="PB780_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDE0732794.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13963..14739
                     /locus_tag="PB780_000724"
     CDS             13963..14739
                     /locus_tag="PB780_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDE0732795.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14732..15301
                     /gene="rnmV"
                     /locus_tag="PB780_000725"
     CDS             14732..15301
                     /gene="rnmV"
                     /locus_tag="PB780_000725"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HDE0732796.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15294..16178
                     /gene="rsmA"
                     /locus_tag="PB780_000726"
     CDS             15294..16178
                     /gene="rsmA"
                     /locus_tag="PB780_000726"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDE0732797.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16629..17258
                     /locus_tag="PB780_000727"
     CDS             16629..17258
                     /locus_tag="PB780_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732798.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="PB780_000728"
     CDS             complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="PB780_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDE0732799.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18700..19122
                     /gene="mgsA"
                     /locus_tag="PB780_000729"
     CDS             18700..19122
                     /gene="mgsA"
                     /locus_tag="PB780_000729"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDE0732800.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19445..19981
                     /locus_tag="PB780_000730"
     CDS             19445..19981
                     /locus_tag="PB780_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HDE0732801.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20021..21784)
                     /locus_tag="PB780_000731"
     CDS             complement(20021..21784)
                     /locus_tag="PB780_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDE0732802.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21787..23502)
                     /locus_tag="PB780_000732"
     CDS             complement(21787..23502)
                     /locus_tag="PB780_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDE0732803.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(24281..24505)
                     /locus_tag="PB780_000733"
     CDS             complement(24281..24505)
                     /locus_tag="PB780_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732804.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            24696..27473
                     /locus_tag="PB780_000734"
     CDS             24696..27473
                     /locus_tag="PB780_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDE0732805.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            27554..27973
                     /locus_tag="PB780_000735"
     CDS             27554..27973
                     /locus_tag="PB780_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HDE0732806.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            27985..28455
                     /locus_tag="PB780_000736"
     CDS             27985..28455
                     /locus_tag="PB780_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDE0732807.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            28472..29251
                     /locus_tag="PB780_000737"
     CDS             28472..29251
                     /locus_tag="PB780_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDE0732808.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            29238..30059
                     /locus_tag="PB780_000738"
     CDS             29238..30059
                     /locus_tag="PB780_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDE0732809.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            30076..31125
                     /locus_tag="PB780_000739"
     CDS             30076..31125
                     /locus_tag="PB780_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDE0732810.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            31138..32142
                     /locus_tag="PB780_000740"
     CDS             31138..32142
                     /locus_tag="PB780_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDE0732811.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            32775..33794
                     /locus_tag="PB780_000741"
     CDS             32775..33794
                     /locus_tag="PB780_000741"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDE0732812.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            33837..34718
                     /gene="galU"
                     /locus_tag="PB780_000742"
     CDS             33837..34718
                     /gene="galU"
                     /locus_tag="PB780_000742"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDE0732813.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            35018..35395
                     /locus_tag="PB780_000743"
     CDS             35018..35395
                     /locus_tag="PB780_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732814.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            36001..36615
                     /locus_tag="PB780_000744"
     CDS             36001..36615
                     /locus_tag="PB780_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDE0732815.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT        12108 a         6483 c         7569 g        10844 t
ORIGIN      
        1 tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat tcttttttat
       61 ctttgtatgg caataataga caatataacg aaaattattt aatggctttt ggaagaccat
      121 ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca cgaaaaaatc
      181 attattttat gaaaaagaca aaaaaaagtg ttgatttttt aataattatt cattaaactc
      241 ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg tacgtaaaat
      301 gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta
      361 tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg
      421 cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga
      481 tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga
      541 cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac
      601 ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc
      661 ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta
      721 cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga
      781 actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc
      841 atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga
      901 agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac
      961 attagaagat tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca
     1021 ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc
     1081 ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga
     1141 gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag
     1201 ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa
     1261 agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc
     1321 agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga
     1381 ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg
     1441 ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga
     1501 tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga
     1561 tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc
     1621 gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt
     1681 tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata
     1741 tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga
     1801 tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca
     1861 aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg
     1921 cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg
     1981 agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca
     2041 aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa
     2101 agctgtacat accgtagctg gcagacgaga gcgaggaaaa ttctggtttt ttctttcggt
     2161 tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga
     2221 cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga tcattagata
     2281 attgaatgag tgtaaaggaa tggttgatga gattttttat taaaataacc agaatcagta
     2341 gaaaagctac tgcgtagggg atagattttt ttttcatttg attgctcctt tatttttggt
     2401 tcattatcta ataatgtagc gaaacatctg tcagaagtca aaagctaggg ggaaacatta
     2461 gtgaatagtt atatcaaata tgtattaaaa cgtgtctttt tcatgatcat cacgctttgg
     2521 ttaattgcaa caatcacctt tttcttgatg caattgctgc cgggcacgcc atatacgaat
     2581 caggaacgtt tgagtcctga aacaatcgag atgctaaata aacaagtcgg tttggataaa
     2641 ccagttatcg tacaatatgg gatttatctt tctaatttgc ttcaaggaga ttttggaatt
     2701 tccttccaat ttaaaaacca gccagtcgca catttgttag ctggaagaat cggaccatcc
     2761 ttgcagttag ggttacaggc aattatcttt ggaacagtcc tagggacgat tttaggaacg
     2821 atctctgcca tgaagcaaaa tacatgggca gatacatctt ctacactcgt agcgatatta
     2881 ggtcgttcca ttccaaactt tgtatttgct gttttattac aatatatctt tgcaatcaaa
     2941 ttacaagttt tgccaatcgc aaaatgggat ggctttgtgt acacaatctt accaacaatt
     3001 gcgttggcca tgtcgccgct tgctgactct gctcggttta tccgtacgga aatggtggaa
     3061 gtactgcata gtgattatgt ggaattagca aaagcaaaag gattgagccg ttgggaaatc
     3121 gcctttaaac atggtcttcg aaatagtttg attccattga tgactttatt aggaccgtta
     3181 gctgtagcat tgatgaccgg atcactggtg gtagaaaata tctttgctat tcctggtatc
     3241 ggggaacaat tcgttaagtc aatcacaaca aatgattatc ctacgatcat ggctgtaacg
     3301 atcctttact catttatgct gatttttgtt attttgatcg tggatctatt gtacggtcta
     3361 gttgatccaa gaattcgtgt ttcagaaggg agccgaggat aaatggaaat gataccaaag
     3421 cgttatgaaa cagttgcaga tatccctgcg gatgaatttc ttccattaca aaaaaatacc
     3481 gaagaagaac gtgaacagat tactgcacct tctttgaatt ttatccaaga ttcatggcgc
     3541 cgtttaaaga aaaacaaagc tgccgttatt tcaatggttt tactattggt catcattttg
     3601 atctctgtga tcacaatctt tgtttctcct catgatccaa ctgcccaaaa cgtggcttat
     3661 atcaatttgc ctcctcgtat tccgggaatc aatatcgatg gattgaatgg aaaagcaatg
     3721 gttggtggtg aacttgttga taaatatgct caagcaaacg tcccttcaaa cgtaaatttc
     3781 tttttaggaa cagacggttt gggacgtgat gtattaagcc gcttatttat gggaatgaga
     3841 atctcgttgc tgatcgcatt tattgctgca ttactagatg taacgattgg tgtagcttac
     3901 gggttggttt ctggtttgtt aggcggacgt gtcgataacg tgatgcaacg ttttttggaa
     3961 atcttatcag gaattccaaa cctcgttgtc atgattttga tgttggtcgt ttttgaacca
     4021 ggaatttttt caatcgtggc tgctatggcc atcacaaact ggataccaat ggcacgtatc
     4081 gttcgtgcac aaacaatgaa attaaaagat caagaatatg ttttagctgg attgacatta
     4141 ggtgaatcaa gaatgaaaat tgcattcaaa catattttgc cgaatatctc tagtgtcatt
     4201 attattcaaa tgatgtttag tattccatct gctattttct tcgaagcctt tttaagcttt
     4261 atcggtttag gtcttacgcc tccgtcagct tcattaggaa cgatgttgag cgaagggtac
     4321 aagacattcc tgtatctgcc ttatctatta tggattccgg cagtaacttt atcggttatc
     4381 atgattggct ttaacttact tgctgatgga ttgcgtgatg cctttgatcc taaaatgaaa
     4441 gagtgaagac gatgacgaaa attttagaag tgaaagactt agaaatctct tttgatacgt
     4501 atgctggaaa ggtacgagcg attcgcggtg tcaattttca tttgaataaa ggagaaacat
     4561 tggcgatcgt tggggaatct ggaagtggaa aatcagtgac gaccagaagc atcatgcgct
     4621 tgctgtcaag caacgccaat atagatgctg gtcaaatcct ctttaaaggt aacgatatcg
     4681 ttggaaaatc agaaagagag atgcaaaaaa tccgtggtaa agaaatagcg atgattttcc
     4741 aagatccaat gacctcttta gatccgacga tgcccattgg gaagcaagtc gctgagtccc
     4801 ttagaaagca taataaagtt tcaaaaaaag agggacaaaa agctgcatta gaattgctta
     4861 aattagttgg aatacccgaa gctgagaaac gtatcaacag ttatccgcat cagttctcag
     4921 gaggtcagcg tcaacggatc gtgatcgcga ttgcactggt atgttatccg gaaatcctga
     4981 ttgctgatga acctacaaca gcgcttgatg tgactattca agcacagatt ttggagctgc
     5041 tgaaagaaat tcagacaaaa atcgatacat caattatctt tatcactcat gaccttggtg
     5101 tagtagcgaa tgtcgctgat cgtgtcgctg tcatgtatgg aggaagaatc gttgaagttg
     5161 gaacatcgga agaaattttc tacaacccgc aacatcccta tacttgggga ctgcttggtt
     5221 caatgccgac attagatagt gcgaacgatc gattgtatgc tattcctggt tctccaccgg
     5281 atttattgaa cccgccaaaa ggagacgcat tctatccaag aaatgaattc gcgatgaaaa
     5341 tcgatgcaga acaagaacca ccattttttg aactatcaaa aacacacaag gcagcaactt
     5401 ggctacttgc tccgcaggca cccaaagtga caccgccaga agaaatccaa cgtcgctggg
     5461 ctatctttaa agagaaacaa aaacatactt acggtcaagg aggaattgct gatgccaaat
     5521 aaactacttg aagttaaagg attgaaacag tatttcaatg taggaaaaaa gaatgaagta
     5581 catgctgtaa atgacatcag ctttcacatt tatgaaggag aaacattcgg tttagttgga
     5641 gaatctggaa gcggaaaatc cactactggc cgtacgatca ttcgcttgaa tgaacctact
     5701 gatggtgaga tattattcga cggtcaagat ataacgaaga tcaaagataa aaaaggtttg
     5761 acgaaattcc ggcatgatgt tcagatgatc tttcaagatc catacgcttc gttgaatcct
     5821 cgtatgaaag tcagagatat cattgccgaa ggaattgatg ttaatggact agctaaatca
     5881 tctgaagagc gtgcaaaaaa agtcgatgat ctattgagaa ctgttggatt gaatccaagc
     5941 cacggtactc gttatcctca tgaattttca ggtgggcaac gccagcgtat tggtattgca
     6001 cgagcattgg cagtaaaacc gcgttttatc atttgtgatg aaccaatttc tgcactagat
     6061 gtgtcgatcc aagcccaagt ggtcaatttg ttgcaagatc tccaaaaaga acatcaattg
     6121 acctatctat ttattgcaca tgatttgtcg atggttaaac atattagtga ccgaatcggt
     6181 gtaatgcata acggcttgtt attagagatg ggaacaagtg atgaaatcta taatcacggt
     6241 gttcatcctt atacagaaag tttgttatct gctattcctc taccagatcc agaccatgag
     6301 agaaaacgca gaagaatcaa atatcaacct gaaccagatg acggacaagt acgtcaactt
     6361 cgtgaaatcg caccagaaca ttttgtctat gcaactgagc aggaagtccc atattacgca
     6421 aaaaaactaa aacgacaaaa ggaatctctt ctggttgcta actaggaggg acatagatgc
     6481 taaaagcttt taaattccgt atgtatccga cagaagaaca aaaacagcaa ttgattcgta
     6541 cttttggatg tgctcgattt acctataacc atttgctgaa aaagcggcag aaaagttggc
     6601 aacaaacagg agtagccaat ttttcgctca cacccgctac attaaaaaaa gaatacccat
     6661 tcttaaaaga agtagacagt cttgctttag caaatgcaca gcttaatttg gatcgggctt
     6721 ttcgcaatta tttcaaggga cgtgccagct tcccaaagct taaaacgaaa aaaagtatgt
     6781 ggcaatctta tacgaccaac aaccaaaccc gtacgatcta tctggaaaat ggctacttaa
     6841 agttaccaaa acaaaaagaa ctaatcaaga taaatcagca tcggccagtt gaaggatcga
     6901 ttcgctccgc tacgatttct gctcgatata atgaagaatt ttatgtcgca ctactttgtg
     6961 atgtttcgcc agtaaaaaaa gaatctttag ctaaatggat cgggatcgcc tatcatccga
     7021 agacattgat ccaaacatct cgacctctag aagtaacttt accaaaattt caccagacag
     7081 aagaaaagct gcaacatgca cagcggaaat tgaatgtgaa agtccgctcc gctcaccata
     7141 gaaagatcag gttagatcag gcaagcaatt atcagaaaca aaagagaaaa gtaatggatc
     7201 tttatctgaa acaaaagaac cagcgagaag attatcttga acaattatct gggaaattgg
     7261 tcaaacaata tgactactta tttgtagaat cttttccaaa agaagaggca catgctgatt
     7321 tttctattca cgactggcat aaattaataa caaaattgcg ttataagtcc caatggtata
     7381 ataaaaaatt tcttttgatc aataccgacg gagcagaaga aagcaattcc gtaagaaaga
     7441 gccaagtagt agaaaaaatg ggcagacatt cggttatcaa gggataaaaa atgaatgaaa
     7501 ctgataggag cgttagggtt agccttgtta ataaaaagtc ttaatgactt ttgtttctag
     7561 gaatttttct tttgaagaaa agctcactaa ttatgacaaa ccaataaaac aatagcagaa
     7621 aaagttcgta cgagcttttt ctgctattgt taatttttta ttcagaaagt ttacgaatac
     7681 atcaaaattc tgaaacaaaa aacgtaactc acaaagagtt gtttttgcgt tttttcttga
     7741 atgattaggt ctgaatcttt tgctcacaac cttattcatg gtgccccggc ttgagctgat
     7801 agatttttga aggacggccg attcctacag gacgttcacc gatctcatcg aaatattgga
     7861 gcatagcttt cttaaaattg gaatgatcga tcgttttata atcgatccct aagaatttag
     7921 caaatacttt tcgagcttcc gtaatcgtga aatctttacc taaaacttgt agaacttgcg
     7981 gctcgtgttc cattttattg actacacggt tgaatgcttt caagatgatt tgactatggt
     8041 caaaagccaa agtatctttg ccagaagact ctccagtgac taaatcaagc gagatagcca
     8101 cttctccgct agataaatgg atcttgttac tatgacgttc tagtgtgaac cagcgtactt
     8161 ccttggcatc atctccggcg attagcggtt cttctccaat aaaggctaag tagctgactg
     8221 tgacgaccca gcctcgcgga tctcgattag gcgtactgaa tgtatgcagt tgctcaatat
     8281 tttcttttga aataaccaca ccagtttctt cctttgtttc tcttaagacg ctttctccag
     8341 ttgactcatc cttttggaca aagccaccag gcaatgccca tgaatttcga taaggatgcc
     8401 cttttctttg gatcaacaaa actttcagct gatcttcttc tttgttatag cacatcagta
     8461 cgatatcgac agtcaaagag ggcttgtcat attctggacg ttcctgtttt tgataccagt
     8521 taagaaattc ttcttcactt gctttatgtt cataatataa tttttcttct tctttagaag
     8581 caaaacggtt catctgacca cctccattga cagtaaaagt actttttacc ttattgtagc
     8641 agactctgaa aaaaaaccaa ggatttactt gaaaaatcta ggttatcatt taaatatgaa
     8701 aaataaatgg aagtatgaga ttgaataaaa agaaaagcag ataaaacggc aatactttat
     8761 accgctcttc acgaatggca aagaagccac aaataacgat atatacgtca atgataaaac
     8821 gcaaaatcca agctgctgtc ataaaactgt tcaaatgccc atggaggtta aaataaccat
     8881 acatacgaat ataaaaatag ctgaatatta aatgtgctaa tgaaagaaaa gcaatactaa
     8941 gtgtaaaaaa aagatgtttc ttctgcatga ttgttccagt cccttactaa gatagatagt
     9001 ctttattata ccacgtgtga ggggagaaat aaaaagccgt tttttagtag atatgaaaca
     9061 aaacaaaaac ccgcatttat tcaacctatt catcctttta aaggatagat tgaacgtaga
     9121 tagaagtcgt ttatccagtc tttttcattt tgaaaaaaag ctaacagcat gcttttttta
     9181 tggtatacta gcaaggaaac gaattcgcat catgcgataa aggaggaaga gccccgtggc
     9241 agaaaaggaa actttttata tcactacgcc aatctattat ccaagcggca aacttcatat
     9301 cggcaactcg tatactacga ttgcatgtga cgcaattgct cgttataaac ggttgatggg
     9361 ctttgatgtg ttttatctca ctggagtgga tgaacatggt caaaaaatcg aaacaaaagc
     9421 agaagaacta ggtgtaaaac cgcaagaata tgtagataaa atggcggctg atgtcaagaa
     9481 attatggaaa acgcttgata tcagttatga taaattcatt cgcacgacag atgattacca
     9541 taaagcagct gttcaaaaaa tatttgatcg tttattagaa cagggagata tttacttagg
     9601 tgaatatgaa ggctggtact ctgtttctga cgaagaattc tttacagaaa cacaacttgc
     9661 agaagtgtac aaagatgacg aaggtaacgt gatcggtggt aaagcgccaa gtggacacga
     9721 agtagaattg gtaaaagaag aatcatactt cttccgcatg agcaaatacg cagaccgtct
     9781 attggaatac tatgaagaac atcccgaatt catccagcca gaatctcgta aaaacgaaat
     9841 gatcaacaac tttatcaaac caggattaga ggatcttgca gtttcacgaa caacttttac
     9901 ttggggagta ccagtcaaga gtgatccgaa acatgtggtg tatgtatgga tcgatgcatt
     9961 atccaattat attactgcac taggatatgg ctccgaagat gaaagtctat tcgaaaaata
    10021 ctggcctgca gatgttcaga tggtcggtaa agaaatcgtt cgctttcata cgatctattg
    10081 gccaatcatg ctgatggccc ttgatttgcc tctacctaaa aaagtatttg ggcatggctg
    10141 gctattgatg aaagacggaa aaatgtctaa atcaaaagga aatgtcgttt atcctgaaat
    10201 gcttgtggaa cgttatggtt tagatgcact ccgttattat ttgcttcgtg ctgttccgtt
    10261 tgggtcagat ggtgtcttta cgccagaaga ttttgtttcc cgtgtcaatt atgatctggc
    10321 aaatgatcta gggaacttat tgaatcgaac gattgctatg atcaacaaat attgtgatgg
    10381 actcgttcct gactatgcct cacttgtcac tccatttgat agtgaactgt ctacgacggc
    10441 tgctaacgtt gtcggacgtt accatgatgc aatggaaaaa atggaattca atacagcatt
    10501 ggctgaaatc tgggtgctga tttctcgtgc aaacaaatac attgatgaaa cagaaccatg
    10561 ggtattagca aaagacgaag aaaagaaagc tgaattgaat agtgtcatga tccatttagc
    10621 tgaatctttg cgtatcgcag cgatattact tcagccaatc atgacagaaa cgccagagaa
    10681 gatcttcaat caactaggat tggactctga aacaatgaat cttgaaggat tacactttgg
    10741 cgaatttcct tcaggaacaa aagtagttgc aaaaggtaca ccaatcttcc cacgtttaga
    10801 tatggaagaa gaagttgctt ttattcaaga aaaaatgtct gaaggtactc aaacaaacga
    10861 agatacagtc aaatgggatc cagaagaaac agaacttgtt tcaacgaaag aaaaacaaat
    10921 caaatttgat gtatttgaaa aagtagaact aaaagtagca gaagtcatca actgtcaaaa
    10981 agtagaaggg gcagataagt tgctgcaatt ccgactagat gctggagaca gccaagaccg
    11041 tcaaatcttg tcaggaatcg ctgaatttta tcctgatcca agcgaattga tcgggaaaaa
    11101 agtcgtcatt gtagcaaatc tgaaaccacg aaagatgcgt ggacaaatca gtcaaggaat
    11161 gatcctttct gcagaagcac cagatggctc tttacaagta atcgaagcac caaaatccat
    11221 gccaaatggc tcagaaatcg catagaaatt caaagggggt atgacactag ttgtgtcata
    11281 cccccttttt ccgaaatttg agaagcaatt ttcgtaaatt ccttcttatt acttgaagcc
    11341 aatcatataa tacagaaaca gagaactatc caaaataaat cctagaaaaa gaagtacgag
    11401 cttcgctgat ttttgttaat ttagaataat actaatagga atcgtaaaaa cagaaaaaaa
    11461 gcataatgat aaagggtcgg gcagatttta attgattcat ctggtactct tttttattgt
    11521 ccttacattc ataaagtttg aatgcaatga ctttattaca tctatattta tatctaacag
    11581 tagcgttgat gtgttaatta taagttctat cactttatgt tcaattagtt tgtgctctta
    11641 tatgtgtgat tgaaaatata gataaaaaaa caatcatcgg ctttgacttc gatgattgtt
    11701 ttctttattt tgtaaaaatc ctatcacttg attttatggt aaccttttat ttttgtcttc
    11761 aaacggcttg gtaagagagc agtaagcagt gcaaatggga tcgaaaagac ataccagaaa
    11821 atcgtacgtc cgataccata atgttgatag agctttgtca acatcacgtg taacaagatc
    11881 aaccctaaaa gcaaggagcc agctataaac caatgattat cccaatgcat gacttcacta
    11941 agtgccatca ctccaaaagg atagaaataa aaatagatcc cgactaaggt catccgccag
    12001 ttctttgtaa atgttccgat attcaataaa aagaaaatcg ctgaaagcaa aataccgata
    12061 ggcggaaaaa gcccaaccaa aaaggaatac aacatccccc acaagatcat cacaggacct
    12121 ttgatcaatg caacgattgc gatgaacaaa gtcagcaata ttagtttttg tgataacgtt
    12181 gcaacagtca ttaatccaaa taaaattaat gtgaaccaat gccaatggga ctgctgttgt
    12241 tctttacggg aaaaatcgac ccggttggat aaataatctg ctaatttcgg ataagaacgc
    12301 ttaaaatcca taaactgttc ctccttcgtg gaaaaggtag gtttagtata cgaaaaataa
    12361 aaaagaaata catccgtctt aagagggagt ttttcactga aagtggagaa aaaagaatgg
    12421 taaattaaaa tattctcagt tatactgaaa attataccaa atttcaaaga agaaagagga
    12481 tgggaatgaa aggcaaacta aaacgagaaa tcaatctgtt tggcgcatta gctaccgtta
    12541 tgggaaccgt gatcggtgca ggcgtcttct tcaaaactgc tgctgttact gctagtactc
    12601 agtctacaag tttgacattg ctagcttggc ttttgggtgg tttcttaacg atctgtgctg
    12661 gattgacagt ggctgaatta gctacagcaa tcccggaaac tggtggagct gtaaaatata
    12721 ttgaagctgc gtatggaaaa ttgcccagct ttttattagg atgggcgcaa agcttgattt
    12781 actttcctgc taatattgct gcattatcga ttatctttgc gacacaattg acgaacttat
    12841 tacaattatc taccgattat cttttgttga ttgcagtgat caccgctgtt tctgtgacag
    12901 ggttgaattt gttagggaca aaagtcggtg cttctgtaca atcagtgacg ttgattgtta
    12961 aattaatacc aattgctgtg attgtcatat ggggattatt gaccccagga caaggaactg
    13021 tccagttatt tccaatcgaa gctggaaaag atgttacttt tgtcgaagga ttgagcagtg
    13081 cgttgttagc aacgttgttt gcttatgacg gctggttagg tgtcggtgca atggcagggg
    13141 aaatgaaacg gccggaaaaa gatttgccta aagcaattat tcttggtttg agttttgtaa
    13201 cagtcgttta tctattgatc aattttgttt ttttgaaaac tttgccgatt gatcatcttg
    13261 ccggtaattt gaatgcagct tctgaagcat cagacgtcat ttttggagga atcggtggaa
    13321 agctggtcac tattgggatc ttgatctcgg tatacggtgc attgaacggt tatacactaa
    13381 ctgggatccg cgtgccttat gcgatggctt tagaagacga gctacctttc agtaaacagc
    13441 tgacaaagct ttcaaagaag tttacggttc cttatgttcc tgcggttttc cagttagcag
    13501 tagcttgtat tatgatgagc cttggttctt tcgacttttt aacagacatg ttgatttttg
    13561 tgatgtggct attcagttta ctgattttta tcggtgtctt tattttaaga aaaaaagcgc
    13621 cggaattgcc acgtccatat aaagtaccac tttatccaat cgttccgatt attgcgattc
    13681 tcggtgctat tttcatttta ggtatgacga tgatgacgca aacgaaatta gcattgatcg
    13741 gtataggtgt taccttgata ggtatcccag tctattatca aaaaaagaaa aaacgctcag
    13801 aagagtaaaa tatttcaaca acagacagaa aaagtacgaa ctaggtcctc tagatcttcg
    13861 tgctttttct atttcaataa aacaagttct tctattttga tttttgctac ttctgcaagt
    13921 agtgcttatg tgctaaagta agaatgaaaa ggagagataa acatgatatt tgattctcat
    13981 acccatttaa atgctgaaca gttcaatgaa gatattccag aaacgattca gcgagcacaa
    14041 gaaatggggg taacgaaaat ggcggtcgtg ggcttcgata cgccaacgat tgaaaagtct
    14101 ttgcaattaa gccatgacta tccaaatata tacagtatta tcggctggca tccgacagag
    14161 gctggcagtt atacgaaaga aatcgaaaat aaattacaag aacaattgac tttaccaaag
    14221 gttgtagcat taggggaaat tggcttggat tatcattgga tggaagaccc gaaagaagtc
    14281 caagataaag tatttcgcag acagattgct atcgcaaaag aaatgaacct accaatcagt
    14341 attcatacta gagaagcact tgaagacacg tatcaaatcc taaaagaaga agatgttcga
    14401 gatatcggtg gaatcatgca tagtttcagt ggtgattatg aatgggcaaa acgatttctt
    14461 gacttaggga tgcatatttc atttagcggt gtcgtcactt tcaaaaaagc attagacgtc
    14521 caagaggctg cagcaaatgt accaatcgaa cgattacttg tggaaacaga tgccccttat
    14581 ctggcaccag ttccttttag aggaaagcga aatgaaccag gctatactcg ttatacagta
    14641 gaaaagattg ctgaacttcg gaatcttccg acagaagaag tggctgccca aacatggaca
    14701 aatgcgcacc gattatttgg tctaagcgaa catggataag atgaagatcc aagagattat
    14761 agtcgttgaa ggaaaagacg atacaagaag gctgcgggaa gtagttgatg cagatacgat
    14821 agaaacaata gggtctgcaa tcaatgaaga gatcctgatg caaattgaac atgcccaaga
    14881 gactcgtgga gtgatcgttt ttaccgatcc tgatttttca ggagaaaaaa taagaaaaat
    14941 cattatggaa gctgttcctc aggcaaaaca tgcttttttg ccgcgcacac aagctaaacc
    15001 aaagaaaaag ggaagccttg gtgtcgagca tgcaagtgat gctgctattt tagaagcatt
    15061 gaaaaaggtc gtgacgccag acatttccca aacggctgtt tctgaaatca ctagacaaga
    15121 ccttttagac tatggtttaa tagctggggc acaagcaaaa gaaaaacgag aaaaactagg
    15181 tgacgagttg cgcatcggtt acacgaacgg caaacaattg gaaaaacgtt tgaagatgtt
    15241 tcgtattacg ccgaaagaac tcgcccaagc aatgaaaata gtggaggaac aacatgagtg
    15301 aatatcgtga aattgcgaca ccctctagaa caaaagagat tttattgaaa catggttttt
    15361 catttaaaaa aagtttagga caaaattttc tgactgaacc aaatatttta aggaaaatcg
    15421 tagaaactgc gcaaatagat gaacatacga acgtgatcga agttggacca ggaatcggtg
    15481 cattgacgga acaattagcc aagcatgcaa aacaagtcgt agcatttgag atcgatgatc
    15541 gtttgatccc tgtattggct gatacgatgc aaccctatga caatgtgact attgtacatc
    15601 aagatatttt aaagaccgat ttgtcaacag ctgttagaga aacatttcat gaggagcttc
    15661 cgttaaaagt cgtagcgaat ttaccttact atattacgac accgatcatg atgcattttc
    15721 ttgaatcaga tttggttgtg gatgaactag tcgtcatgat gcaaaaagaa gtagctgatc
    15781 gtatctctgc tgaaccagga acgaaagctt atggttcatt atcgattgct gttcaatatt
    15841 atatggaagc cagtttagca ttcatcgtac caaaaacagt atttgtccct cagccaaatg
    15901 tagattcagc gattttgaaa ctgacaagaa gagatatacc ggcagtcgaa gtgacagacg
    15961 aaaaagcatt tttccgattg acaaaagcgg catttcagca aagaagaaaa acgctttgga
    16021 ataacttgca gcattcgtat ggcaaagatg accagacaaa agcatggtta gcaaaaagct
    16081 tggaaacagc tggaatcgat ccaaaacgtc gtggggagac tttgtcatta caagaattcg
    16141 ccgcattaag caatgcgatg tcagaaaata tgcagtaaaa aaagaggttg tgacagaagt
    16201 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata gttcctcatg
    16261 tttttcgttt gtttgggctt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    16321 aggttgtggg cctgccgttt aactctaagt cacaaggaac aatttcaaaa aacagcttct
    16381 catgtttttg agttagttgg tcttattact tgatgcaagc ttttgaccac tattatgccg
    16441 aacaaggaga tgtgacacaa attttgtcac atctcctatt tttgtttctt gatcaaaaaa
    16501 ttcttatact tgatactcac actcattttg tatggattta gaaaagaata tgtgtcttga
    16561 cagttattag taagtgagtt atactaagtc gacatttatg tgtcaagaca tattaggaga
    16621 gagaaaaaat gagaaaaaat tcagttcgaa aactaacgat ttctgcttta ttgattggga
    16681 tgggggtcat tatcccaatg gtcatgccta aaatcatgat tccaccagct tcctttactt
    16741 tggcgtctca tgtgccatta tttattgcga tgttttttac accaggagtt gcagtggctg
    16801 ttgcgctagg tacgacattt ggtttctttt taacaacgcc ggttatcatc gcattgcgag
    16861 cattgtcaca tcttgtgttt gcgttgattg gggcttggta cttgcaaaag catccgagta
    16921 tcgttttaaa aaatggacag tttacttttg ctaattggcg tttccaaggg ttcaattttg
    16981 tgatcggtgt gattcattct ttagcagaaa tgctagtagt cagcatcttt tactttattg
    17041 gtaatatgcc tgaaacatat tattctcaag gatattttta tacagtattc atcttgatgg
    17101 ggattggtgg gcttatccac agtttagttg attataatat tgcttatttt atagcaggaa
    17161 cactcagcaa atattttgat atccccgtat ttacggcagc aaaaaaagaa aatcggaaag
    17221 agtcgattca agggtacgcg aagaaaacga catattagac atgatctctc aaaaaaaaaa
    17281 ctctcatcta gtaatggata ataaatcctc taagatgaga gttttttgtc ctaaaaagag
    17341 ctgcaccaac atcttctggc acagctctaa cagggggcaa tttatggaag tttgatgact
    17401 tgttctgttt ctttatcaaa gaagtggcct ttgctgatgt tgaaggcaag gtcgactatt
    17461 tcgcctggtt tatggaaatc tcttgcatct acttttgaga tgaactcagt agaaccagtt
    17521 tttgtgtaaa gcatcgtttc agctccaagt aattcggaaa cgacaacttc tgatttcact
    17581 acggcttcag gtgaagcttc aagggctact tgttcactgt ggatgtcttc tggacgaata
    17641 ccgaagataa cttcttttcc ttcataacct ttttcaacta atactttgtt ttttccttca
    17701 ggaatacgaa gcttaagccc atgtccatct gtgataacac catttttcaa tgtcacatta
    17761 aagaagttca tggcaggaga tccaataaag ccggcaacga acatattgac gggtgtatca
    17821 taaacttctt tcggtgtacc gatttgttgg ataaatccgt ctttcatgat aacgattcgg
    17881 tcagccatag tcattgcttc tgtctgatcg tgagtaacat agatggtcgt tgtttcaaga
    17941 cgctggtgaa gtttggcaat ctcagcacgc atggctacac gtaatttagc atctaaattt
    18001 gataatggct catccattaa gaagactttt gcatcccgaa cgattgcccg tcccaatgcc
    18061 acacgttggc gttgtccacc agataaagca gctggttttc gtttcaagta ttctgtcaga
    18121 cctaagatct ctgctgcatt gtccacacgt tttttgatgt ctgctttatc atatttacgt
    18181 aatttcaagc caaaagccat attatcaaaa acagtcatat gaggatacaa tgcgtagttt
    18241 tggaaaacca tggcaatatc acggtcttta ggtgccacgt cattcatgac ttttccgccg
    18301 atatttagtt ctccttcaga gatatcttcc agtcccgcga tcattcgcaa agtagttgac
    18361 tttccacaac cggaaggacc aacaaaaacg ataaattcac ggtctttgat tgatagatta
    18421 aaatcagtta cagaataatt ttctgcattg tcatattttt tatatacgtt tttcagagcc
    18481 atttctacca taatacgttc cacactttct tttttagttt tattaactat ttaaagtata
    18541 aatgaaagcg ttaactaact tcaatgtcat gttgaacaag aattttttct tttttttgtc
    18601 aatgtgctag ttttaagctt taataaatat ccataagaaa taaaaactga tacactggag
    18661 taaataagta tgttaacaat atcaaaagga gtgtctctta tgaaaatcgc tttgatcgct
    18721 catgaccgaa aaaaacctat gatggtgaaa ttagcaacag cgtataaaac aatattggaa
    18781 aaacatgaac tttttgctac cgggacgact gggcagagga tcacggaagc aaccggcttg
    18841 ccgatccatc ggtttaaatc cggaccgtta ggcggggatc agcaaattgg agccatgatt
    18901 tcggaggatc agttagattt ggtgattttt cttcgagacc cgctggctgc acagccccat
    18961 gaaccagatg taacagcact tatccgttta tctgatgtct atgagattcc gttagctacg
    19021 aatatcggaa cagctgaggt gctactaaga ggactggaag ccggcttcct agattttcga
    19081 agaatcgtcc atgaaataga caagcatcca ctttcatttt gatggataag cgtatggaaa
    19141 aataacgttt aatgttcgcc tatatgtagc tataaaagct aaacaaactg ataatatgcg
    19201 atataatacg aacgtattgg ataatataaa caataatatt agtttttcgt atttgcagta
    19261 aatgtgaaaa tatgctatag tttctgtggg aaaagatccc aaaaataata cagagtagaa
    19321 aataaagcgt taagtgctaa gaggatggga tgttgcctct tggacgaagg acgtaatgtt
    19381 cgcggtttta ttttcgcatt cgctgcatga tacgatgtag cagctcttaa aggagatgct
    19441 aaaaatgaag aaacaccggt tagacgcacg tatgattgct attatgggct tattgattgc
    19501 attgatggtc actttgtccc gactggtagc aatcgagacc ccatttatta aaatcagtgt
    19561 cacatttatt ccacaagtga tcatgggcat tctttttggt cctttttggt cagggatagg
    19621 cgcagtttta gctgatttag taggaatggc cctcttctcg aaatctgcat tctttattgg
    19681 atttacattg aatgcattta ttgaaggagc aatctacgga ttctttttct atcgaaaaga
    19741 gatcacttgg aaaaatgcca ttctggctac gcttagtgtc acgctgatca tcaacttatt
    19801 tttgacacca ttatggcttg cgttgatgta ccatgtgcca ttgttcagct gggtcgtttg
    19861 ggcgccccgc ctgttaaaaa cagtaatatg gttaccgatt caatcaattg ccatttatta
    19921 cgtaggacgt agtattcctt acaaaaaaat tttaagaagt ttagcgatcc acgctaaata
    19981 aaaaacaagc agtcttccca tcccagaaga ctgcttgttt ttattgtgct gtgaattgtg
    20041 cttgatagag ccgtcggtaa taaccatctt tttgaagcag ctcatcatgg gtgcccattt
    20101 cgacaatagc tccctgatcc atcactaata tctggtccgc atttttgatt gtggacaggc
    20161 gatgggcaat cacaaaactg gtttttcctt ccatcattgc taagaaggct tgttggattt
    20221 ttttctctgt caatgtatcg actgagctgg tagcttcatc taagatcaac atagggggat
    20281 tactgatcat cgttcgagca atcgtcaaca gttggcgttg tccatctgat atctttaacc
    20341 cttgaccgcc aatcttagta tctaattttt ccggcaaacg ttctacaaat tcatacatat
    20401 aagaggcttt tagggcatca ttgatttctg cgtcacttgc ttccggattc ccaaaacgaa
    20461 gattttctcg caatgtactg tcaaatagcc aagtatcttg taacaccatt ccaaaacttt
    20521 tacgtaatgt atcacgttgg atagagcgga tgtcatggtc atcgactgta attgttcctt
    20581 ttgttgtgtc ataaaaacgc atcagcaaat taacaagtgt ggattttccg gcccctgttt
    20641 ttcctactat agcaatggtt tgacctggat aagcagtaaa attgaaatct tcgatcaaac
    20701 gctgcttggg gtcataagaa aaagaaacat gatcaaaaga gacttttcct ttgatatttt
    20761 tgggttccaa ggcataggca tctggcaaat caggcttttc ttctggctga tcgatcaaat
    20821 caaatgctcg ttctagacca gaaaaagcgg tttgcagttg tgtcgtgatt ccagaaagtt
    20881 cgataaatgg tttagaaaac tgacttgagt agatcgtaaa gctggaaatc acaccaacag
    20941 tgattgacga accactcttc aatgccaata ggccgccaac taaaccaatc gataaataag
    21001 caaggtgatc tacaaaacga gaagacgggt ttgtcaagga agaagaaaat tgtgcccgtt
    21061 gtcctttttc atatagatca gcatttagca gttcaaattg ctgttgacta gcttcttctt
    21121 gctgaaaagc tttcacgatt ttctggttac cgattcgttc ggtcacaaag cccgagatat
    21181 ccccaataat cctttgctgt ctatcgaaat ttgtttgtga agcacgagca acgatccaac
    21241 tcacaaggaa aataattggt gtagaacaaa gaacgaccaa tgtcagaagt ggacttagac
    21301 gcatcataaa aataaacgaa agaatgatga ttgccactcc agaaaataat tgattgaagg
    21361 ccgcagaaat tgcaatggaa atattgtcca tatcatttgt aaatcgactg acgatatttc
    21421 cgtgggagtt ttgatcataa taacgaagtg gcaatcgatt cagatgagca aatgcatctt
    21481 ttcttaattt tgcaacggat aagtaagcta accgattgct taacacttgg atgagccatt
    21541 gactgactac gttgattaaa acgataccac cgaatagaat aagtaaatgg aacagtcgat
    21601 tgaagcggac ctgtccttct ccaatcattt gatcgatact ttgaccaatt tgtaacgtca
    21661 tcacaactgt tgcaatccca ttgatgattc ctagtatcac agctagccaa atgtcttttg
    21721 cataggcagt aaggtaagga atgaatcggc ggagcgtctg caaggataat ttttttactt
    21781 tcataactat tcttcctcct gtgatgcgac gatttcctga tattctgctg attgagcaag
    21841 taattcttca tgggtaccct gagcagcaat cttaccttga tccatcacta agatatgatg
    21901 tgcttcttga atagagcgga cacgttggga gataataatc actgtactgc caagataagt
    21961 ctgtagcgcc ttacgcaaat ccaagtctgt ttgataatcc aacgcactta aagagtcatc
    22021 taaaatcagt aatgctggct tggcaatcaa agctcttgca atcgtcagtc gctgtttttg
    22081 tccgccagag aaatttttcc cattttccat gacttttgta tctaaaccat ttggaagctg
    22141 acggacaaat tcttctgcct gagcgatttt cagagcttcc caacattctt tttctgtggc
    22201 gtctttctta ccccattgaa ggttttcacg aatagtcccg gtaaatagta cagaagtttg
    22261 gggaacaaga gcaatttgct gacggagatg atccagctgc caagaacgaa catccattcc
    22321 gtccattata actgtaccgg aactgacatc atagaaacgc ggaatcaatt gtgtcaaagt
    22381 cgttttcccg cttccggttg gaccaatgat tccaaggact gtatttcgct tcagctgaaa
    22441 tgtaatattt tggagagaaa gaccagaatc aggagtataa cggaagtcaa catggtcaaa
    22501 agcgataatt gcttcagaag aaaaatcggg agtagtactg acctcagctt caagaatcga
    22561 attttctgtg tctagcacct ctttcacccg atttcctgaa gcttcggcac gtgtgaagat
    22621 cacgacgagg tttgaaacaa cgatcagtgc caaaagcatt tgattcatat aattgatcaa
    22681 agcaagaact tgtccttgtt gcaagctacc aatatttact ttgatcccgc caactgtcag
    22741 caaacagatg atcccgacat tcatgatcaa ggttgtagca ggtgataata gggcagagat
    22801 attggaaaca cgtaaataat tgttcgctag atcatttgtg cttttatcga acttctctgt
    22861 ttcttttttt gtctgcgcaa atgcgcggat gacacgaaca ccgctaagat tctgactaac
    22921 gtgttcattg agctgatcta gtttttcttg cactttttgg tagagaggaa cggttttttt
    22981 gatgataaaa aataaaatga ttgagaaaat aggcaataat gctaagaaaa acagacctac
    23041 ttcccagtcg atcacaaaag ccatgaccac tgacccgata cttaaaaacg gtgctctgat
    23101 caataaacga atgaccattg ctaaagctag ctgaagctga ttgatgtcgt ttgtcattcg
    23161 ggtgatcaag gtatctgttc caaaatgatt caactcgttg tgagagaaag tattgatttt
    23221 tttgattagc tgattacgta actctgtacc aaatccttgt gaagctactg aagcatagta
    23281 ttggcaaatg atagcacaaa tcagaccaat cacagacata gcgaccatcc ataagcccat
    23341 ttcaatgatt tttcctcggt cattcatttt tagtccatta tcgatcaagg aagccatcat
    23401 taaaggcagg acaagttcga agacagcttc aagaaatttg aagaacgggc ccaaaatgat
    23461 ttgcttacga tagtcttttg cataacgtaa taactgaatc atatgattcc tcctattatt
    23521 tatttgtcca caattgaaaa aacaagtact accacattgt agcttaggaa atttagaatg
    23581 aaaagagaac aagccttttc ataaatgaaa gttttattat gaagaagctt tagaaattga
    23641 atagactatt ttttttaaaa aaaagaaaat gagaaaacat gccagtctct tcattcatag
    23701 gaacgtctat tttacttaat ttttctataa atgaattctg cataaaaaat atatcaagta
    23761 aatgtttata gatgaaactc tatttacttc ttttttaatt tgttcttgca atcttttttg
    23821 ctacgtaatt tttgtaaaaa agtgggttga gagaaatcta attgattttc aaagaaggag
    23881 tatgaaaatg agaaaaatat ttattcgttt attgtgggta ctatcttaga ggaataaata
    23941 atagtggaga aaactatggt ttggcgcaga ttctatcagg tttgatggaa cgaagacaac
    24001 ctcccgacat caagtcaaaa gctacttgag attttgatct gaaaataaaa aaagaaaaca
    24061 aggtaggaac atcaatgttt ctaccttgtt ttcgtaatca atgggaaaaa actaagagtg
    24121 gtgtacgtaa tcaataaaaa ggatggtgac aacagtacct ctttactctc tgaaagcatc
    24181 ataactcgtt tttcctcatt atgccaataa attgttgtta taccacatat aaggatttgt
    24241 aatagctaaa aacagacagg aaagttgaaa tctgtgaatc ttattttgta tggaaggatt
    24301 tatagaacgc aaaaatcaag ataaagatga ataaaaatcc tgtttcggct atatattgcg
    24361 tatctacgcc aataaaacga gtttgttctg tcatagccat ataaagcaaa ggcaaaaata
    24421 caactacagc gagataagat ccgatgattt ctcgaaaagt tttccaggaa gtatgtttcc
    24481 tgtttttata tttatccgat ttcattgaat agtcatagcc tccttattct ttcatatgtt
    24541 gaaattccag tataaaaact ggaaggaact gtacgttttt tagaataaaa attataaggg
    24601 ataagtaggc tgctgtctat aataagatat atttttcaat tctttattaa agattttatt
    24661 gacaaaatga atccgttttc tatgatggag gaggggtgaa taagttgttg aagaaggaaa
    24721 ttttatgtta cttagagaac cagacagcct ttttcgattt agaacatatg aacgaggtct
    24781 ttacagcaca aaggttagct gatcattttg gggtaaaaag aaacacagtc agtcattatt
    24841 tgaaccagtt aaatgatgag caactactag tcaaaatcaa ttctcgacca gtgatttatt
    24901 tccacaaaga agcttttgaa aaacaatttt ttaagttacg cacgaatttt tatttttcta
    24961 tcgatgatct caaagaggaa cagccttttt tcgcgcaacc aaaagactta ttctctttta
    25021 tgatcggata cgacgccagt ttgaaagaaa gtatcgaaca aatcaaaaca gctctttact
    25081 atccagatgg cggattacca cttttgatta caggcgaaag tggcacaggt aaaagttatt
    25141 tagttaattt ggtctatcag tattgtctgc ttcatgatct gctagaagat tctgcaccat
    25201 tcatcacttt taattgtgca caatatgcag ataatcctga acttttgact agcaatttgt
    25261 ttggacatgt taagggagcg tacactggtg cagaagaaaa taaaaaaggg gcttttgaag
    25321 cagcagatgg tgggatcttg tttttagacg aggtacatcg cttaacacca gaaggtcagg
    25381 aaaaactgtt cacctatctt gatcaaggtg tgatttatcg tatgggagaa cctaatttgt
    25441 cacgccggat caaaacacgt ctcttttttg ctacgactga agagattact agtcatttct
    25501 taacaacttt cattcgaaga atacctatcc gaatcgaact acctgcactt aatcagcgta
    25561 gtcgtaatga acggctagaa ctgatttact ctttttttct cgaagaacaa agaaaaatgg
    25621 ggagcccttt gtcagtcagt ggaccagctt tgtcactttt aacaagtcag aaattatccg
    25681 gaaacatagg agagctgaaa aatatcgtca aagtttcagc agcaaaagca tatgctgaac
    25741 agcgtgaaca gcctgaaatc catgtcacga tccatcattt gcaaaaagaa ctacttgcgc
    25801 agcctattac taaaaataca gcacagcaag gaatctatat tacgcaagac cagaccttag
    25861 agcaattaat ggagaagcaa cagcccgaac agcagcgaat cgttaaaagt tttgagcaaa
    25921 tcctaattga tttcaagaaa aataactgtc tgctatattc ttgcgaagga aaattaaagc
    25981 aagaagtgat tcaattattt gattttttgc tgtttgagac cagtagacaa gaaaaacacg
    26041 agctgctggt ttattggaca caatctatcc gagagacctt tcgacaaatg gaaactgcct
    26101 atcaaatcaa attcgatgga aacagtgtct atgccttgag ttactatctc tatcagcgaa
    26161 gcactatcaa atggttaccg gaagaacatg atattatcca actaatcaaa gaattagaac
    26221 aacaagtttc tcagtcttat ccatctagtt atcactatgt ccaacgaatt ctggaattaa
    26281 gtaagatcaa attagatatt gaagacacaa gtatggatcg gattttactg acgatctatc
    26341 taaaaaaagc gaaatggtca aaagaaaaac gactgccaaa agcgatgatt gctgctcacg
    26401 gttatgcaac tgctagcagt atggcaaatg tagtaaatcg tttattacaa gaggatctat
    26461 ttgaatcatt tgatatgcca ctagatgtca caccacagca gattgcttca gaaatccttg
    26521 attattgcga aaacagcgat gtttcaaatg gattgatcat attagtagac atgggatctc
    26581 tgaaagaaat ccatcagttt tttaaaaaac agttatccgt accgcttttg attttgaata
    26641 atgtgacgac acctttagca atcacagtag gggaatgcct gcaaaaaaat gtatcattag
    26701 aagagatagc agaagagact gttcgacaga tccagccaga atggcgactg ctctatccag
    26761 aggaaaacaa acccaaagca ctcattacta cttgcttcac tggaatcggt acggcggtcc
    26821 acttaagtga actgctagaa aaaagcttac ctacggcttg tcagttgaaa atcatcccat
    26881 atgaatatca gcaattaaaa gataagaaaa acagtgaccc attattttcc atatacgaag
    26941 tcgtaggaat gattggcacc acagatcctg atatcaccaa tatcgactat ctttcattag
    27001 aagacctcat ctcgggggaa aagatgtctg tactagctga atggttggac actactatga
    27061 atgcttcaga gaaggaaata ttcaatcagc gcatcattcg taatttttca ttggaaaaag
    27121 tactggattc cgtgactatt ctagatacag agaaggtgat gggagaaatc gatcatttta
    27181 tgagagaatt agaggtgcag ttgaatcgaa gcatcagcaa tcccaaaaaa ttagcgttgt
    27241 atgtccatgt gagctgtctc attgaacgat tgatccgaca aatgccaatc gaaacctatc
    27301 aaggattaga gaaatggaag cagtgtcaaa aagaagggtt atctgctatt aaatctgcgt
    27361 ttagtgtcat tgaagagcat tatagtgtca tgatccccaa ttctgagatt gcctatatct
    27421 atgatatttt gagtgaagat acagagtttc tctcgataga agaagaattt tgaacataag
    27481 caagttgttg ccactaattt tggcatgcaa cttgcttttt tagtatgtgt aagtgaaaag
    27541 aggagatgaa aaagtggata gagaaattat cttagcttcc catggaaaat ttgcttcagg
    27601 gatattagat tcattagagc tgatctatgg gaaaaatcat tctattactg tattcgattg
    27661 ctatactgat gagaattttg atttagcgga aacagtaagg cagcttttta tgaggtacaa
    27721 agataaagaa atcattgttc tgacggatct ttttggcgga agtgtcaaca acgaattctt
    27781 acaatatatc aatcgggatc atgtctatct cgttgccgga ttgaatttgc cactattgat
    27841 tgagctagta tctcggttgg acgctgaagt agagacaact gttttgatcc agcaggtgct
    27901 aactgattca aaaaaaatga tccagttttg taacgagcgg ctcaatcaga aaatagaaga
    27961 agacgaattt taggaggaag aaaaatgatt gtattgacaa gagtcgacca tcggttatta
    28021 cacggacaag tagctttttc atggacacaa acaataggcg cagactgtat tttgattgct
    28081 aatgatgatg tacctacgaa tgagatacga aagacaacga tcaaattagc caagccacaa
    28141 ggagtaaaac tagtgatcaa atcaattgat gactcgattg ccgcgttgaa aagcggggtg
    28201 acagataaat ataagttatt catcgtagtg gaatcgatcg aggatgcata taagctggca
    28261 gaagcgtatc cagaaatcaa aaccatcaac ttaggaggga tcaaggcaaa acctggtact
    28321 cgcagcatcg gaaaagcggt aaatatttta ccagaagagg aggaacgctt gaaacgatta
    28381 attgacaaac aaatagaagt agaaatccgt caagtaccag cagataaaaa agtggatgta
    28441 agagaagttt tgtaagaaag gaagaacctc aatgattgta caagcagtct tattaggaat
    28501 tgttgcattt gtcgcacaat cagaatatgc actagggaca tcgttgcttt ctcgtccgat
    28561 tgtgacgggg ctgtttacag gaatcgtgtt aggtgatgta aaaactggga tcattatggg
    28621 cgcaacttta gagttagcat ttatcggatc gttctcagta ggtgcttcga ttccacctga
    28681 tgttgtgacc ggtgggattt tagggacggc ttttgctatt acagctggag ccggtacaga
    28741 aaccgcgctt ttactaggtt tgccgattgc tacgttgaca ttgatattga aaaatattta
    28801 cttagggctt ttgattccaa ttatgaacca taaagcagac aattatgcag aagaaggaaa
    28861 ttataaagga gtcgagcata tgcacttgct tgccggtatc ggtctttcct tgatgcttgg
    28921 tgttgtggta atggtttctt atatggtggg aagcaatacg atcggaaatc tattaaatat
    28981 gattcctgac ttcgttcaaa aggggttgtc agtagctaca gggttgattc cagctcttgg
    29041 ttttgcgatg cttgctcgtt tgctgatcaa caaacaagtt gcaccatatt ttttcttagg
    29101 ttttgctata gcttcttact tagagattcc tgtgaccgga gtagctattt ttggtgcgat
    29161 tttagcagtt gtggtagtca atattatgaa tgtccgtcaa ctaccacttc aaacaactgc
    29221 aggggaggta gaagaagatg aagaattctg agatcaaaaa cagcaaaata acgaaaaaag
    29281 agctaaatca cgtattttgg cgttcttttc aaatggagtt ttcttggaat tatgaacgtc
    29341 agatgaatat ggcttatgtt tatgcaatga ttccgatttt gaaaaagtta tatcagtcga
    29401 aagaagaaat ggcagcagct ttgaaacggc atctagagtt ttttaacacg accccacata
    29461 tcgttacact gattttagga atcacggctg caatggagga agaaaataag gatgatcctg
    29521 aatttgatgt gactgcgatc gatagtatca aaacatcttt gatgggacct cttgcagggt
    29581 taggagattc tttcttttgg ggaactttgc gattgattgc cacaggtgtg ggtacctctt
    29641 tagccatgca aggaaatatt ttaggcccta tcttgttcat tttgattttc aatattccgc
    29701 acattctgtt tcgctatcta gcaacaggtt ggggatatcg cttaggaacg ggttttttga
    29761 aaaagatcca ggcaaacggt atgatggaaa gtttgacatt aggcgcgtct attattggac
    29821 tgatggtcgt tggagcaatg actgccacga tgatcaccat caatattcct ttaaaaatcg
    29881 gttcaggaga aaatgctgtg actgttcaaa gtatttttga tgatatcgtt ccaaatatct
    29941 tatgtcttgg agcatttggc atagtgtttt atctattgaa gaaagaagtg aaaccattaa
    30001 cgatcttgat cggactagct ttctttggaa tttttggttc gtggataggg attttttaaa
    30061 aaaggagcag agaaaatgtt tacgatgcaa gattatatct atgaagaaaa ggaagtacta
    30121 agtacgatct tgaaaaagaa tgatttttcg acaagagaac atatgaaaaa aacagtgaat
    30181 ttactgatct tagcaacggg ttcatcttat aacgcttgtt tagcagcgaa gccagcattg
    30241 gaatcttatg gtgatcttac tgtcgatatt caagaacctt tttatttttt caattatgga
    30301 aaactttctc cttcaatcga tactgtgatt gctgtgtcac aaagcgggaa aagtgcttct
    30361 actgttgaag cagtaaaaat gatacaaaaa caagggttac ctgttgttgc tatcacaaat
    30421 gacgtgcaaa gtccacttgc tttagaagct gcacaaatta ttgatttagg tgccggggta
    30481 gaatcagtag gcttcgttac aaaaggctat tctgcgactg ttttacagtt actactctta
    30541 gggataggaa ttggaataag taaaaataaa atcagcaaaa aaatagaaca agactacatg
    30601 caacagttgc gcaaaataat caaccattta ccagcgatta ttcaaaaaac ggaagaattt
    30661 tttgatggat atcaaagttt atttcgttta gcacagcgtt tcgtcaccat tggatatggt
    30721 ccaaactggg gaacagctaa agaagcagag acgaaattga ctgaaacaat ccgcgttccg
    30781 tcacaaggat ttgagttaga agcttatatg cacggcccat acctggaggc agatgcttcg
    30841 catttgctat tctttattga aggggaaagt gtaaataagg aacgttcgca aaagttgcaa
    30901 agatatatga gtcggtacgt tggagagact ctcaccatta ccacaaaaaa agcaagaaac
    30961 gagaaaacac ttggattagc aatagaatgt gatgaatatc tgtctgtatt agcgcttgtc
    31021 gttccgtttc aactattcgc ttataaaacc gcagtagcta aaggtattga cttgaacaaa
    31081 aagatttttg aagactttga tacagtcttg aaaagtaaat tataagaggt ggaaatcatg
    31141 ctgaaattca atgaagaaga acaaatcaaa gcaataaaag gcgcactagc tttacgtccg
    31201 caagtagaag aaatcatcga taaactttat gcggaaaaat ttgacgctgt ctattatcta
    31261 ggaatcggtg gaacgtatgc ttcctcgatg caagctgtca cttatatgaa tgggaaaagc
    31321 aacttgcctg tctttgtaca gcatgctgca gaatattaca ctactggaaa caaaagatta
    31381 acaaaagatt ctatcgttat tttgtcctct gttaccggta caactcaaga agtagtgaaa
    31441 gcagtagaac aaatcaagaa agtaggagcg acactgattg gatttatcga taaagcgaac
    31501 agtaagttgt ctcagctatg cgattttgtg gtcacttatc cagctcctgg aacagaacaa
    31561 atcaaatttt ttatggcagc agatcgattg atgtatttga atggagaatt cgaagcttat
    31621 tcagaatact atgaacagct cgaacgttat ttaccaacag ggttagtgga agcagagaaa
    31681 aaagcagatg catttggctt atcatttgca gaaaaacatc gacgtgatag tatgcattat
    31741 tttattggag cagggaatca atggggagct gtttattcat atgccatgtg ttactgggaa
    31801 gaacaaagct ggcttccatc gaaatctatc catgctgccg agtttcttca cgggacgtta
    31861 gagatcgtag aagaaacaac cccagtcaca ttatttttag gcgaagatga acagagagtg
    31921 ttatctgaac gtgtggctaa acttttgcca aaaatttgca gcaattatac attgatagac
    31981 acaaaagact atccagtaga aggtatcagt gaaaaatatc gcggacgtgt tttgtctttc
    32041 ttattgatgc atgtagtgac acaacgcatc gatgctcatg tggagcagtt gaactgccat
    32101 ccattagaga tacgccgcta ctatcgtcag tttgattatt aggatagact ggataaaaag
    32161 aatataaaaa ataaagagag cagcgataac tgctcccttt atttttttaa aatattggca
    32221 tcaattacgt tataaaacac attttttgca ttatctacag ttcaacttat aatctgttta
    32281 atgggacatg tgggtgccaa ccattttttg ttatgcaata gtactattta gttgttgcat
    32341 ggatccgtag taaaagtcca agtacaacga gttgcgccag tttacatagc gttttgtatc
    32401 cagaatgttg actaaaacaa aagttcctat gtcaaccgtc aacagagaca atctgtccgt
    32461 tgcaggacct gacagattta gaaatgttca atctgtctct tatgtatttt aaatcaatac
    32521 tcactaggag aagtttccta acctcctaat agtaatgctg gtagtaacat tgtcaaaact
    32581 gcttcctctt aggaacaagc aaagcataac gagtgtaaaa ataaaatgta gactattgta
    32641 acatatcatt gattatttca agtaaaaacg ataaaagaag taaacaaaca cttaaaaaca
    32701 atggaaatta gtcccaactt taagaaatgt atactataat ggaaattatc aattatttat
    32761 ttggaggaat atccatgaaa caaaaaatcg cagttcttgg tcctggatct tggggcactg
    32821 ctttagcaca aacattggca gaaaacgggc atgatgtccg tatttgggga aatgtacctg
    32881 aacagattga tgaaatcaat aagtatcata caaatcagca ttttttacct gatttgacga
    32941 tcccagaatc gattatcgga tataaagaat tggcagaagc agtggataac gcagatgcta
    33001 ttttatttgt tgttccaaca aaagcgattc gttcagtcgc acaggaactt attggtaaaa
    33061 tgaatactaa gccaatcatt atacatgcaa gtaaaggact agaacaagat acgcataaac
    33121 gaatctcaga agttttggct gaagaaattc cagaagaaaa aagacaggca attgttgtat
    33181 tatctggtcc tagccatgct gaggaagtgg cagtacatga tatcacaaca attacagctg
    33241 caagtattga tcaaaaagca gcagcctatg tacaaaaact gttcatgaat gattatttca
    33301 ggatttatac aaataatgat gtgattggcg tagaaactgg tgcagcactg aaaaatatca
    33361 ttgcaattgg tgccggcgct atccatggat taggattcgg agatgatgca aaagcagcaa
    33421 tcatgactcg aggattggca gaaatcagcc gtcttggtgt cgcgatgggt gccaatcctc
    33481 tgacatttat cggtttgagt ggtgtggggg acttgatcgt tacttgtaca agtgttcatt
    33541 ctcgcaattg gcgtgccggt aacttgttag gacagggaca taagctggaa gaagttctag
    33601 aaaacatggg aatggtcgtt gagggtgtgg caacgacaaa agcagcagta gagctagctc
    33661 aacagctagg tgttgagatg ccaattactc aaacaatcta taatgttttg tataatggag
    33721 aagatatcaa aaaagcagca aaagaaatca tgttacgtga cggaaaaatg gagaatgaat
    33781 tttctcttcg ttaaatagat gatcaatagt taatcgtcgt ttaggaggaa cagcaagtga
    33841 aggtcaaaaa agcagtcatt cctgccgctg ggctaggaac acgttttttg ccggcaacaa
    33901 aagccatggc aaaagaaatg cttccaatcg tggataaacc aacgatccag tttattgtgg
    33961 aagaagcatt ggattcggga atcgaagaca tcttgatcgt aacgggaaaa gaaaaaagac
    34021 ccattgaaga tcactttgat gcaaatatcg aattggaaaa caatttgcgg gaaaaggaaa
    34081 aaacagaatt actcgcttta gttgaagaaa cgactcaagt caatcttcac ttcatccgtc
    34141 aatctcatcc aagaggactg ggggatgccg tgcttcaagc aaaagcattt ataggaaatg
    34201 aaccgtttgt cgtcatgctg ggggatgatt tgatgaaaga tgatatccca ttgactcaac
    34261 aattgatgat ggattatgaa gaaacacaag cttccgctgt tgccgtcatg cgtgtaccag
    34321 aaaatgaaac atctaaatac ggaattattg atcctaaaac agatagcgaa aagggtcgtt
    34381 gcagagtaaa agggtttgta gaaaaacctg aaatgggaaa agcaccaagt aatttagcca
    34441 ttatcggacg ttatctactg actcctaaaa tatttgagat attagaaact caagaaccag
    34501 gagcaggaaa cgagatacag ctaacggatg cacttcagac tcttaaccag acagaggcgg
    34561 tctatgcgag agagtttaaa ggaaaaagat acgacgtagg cgataagtta ggatatatga
    34621 agacaaatat tgaatatgga cttcaacatt cggaaattgg gacttcatta agtgcatata
    34681 ttatccagtt gagtaaaaag ctaagaacag aaagttaact ggaaataagt ggcctgctga
    34741 gaatgaaagg ctatttgtta ttaaagcggc agcttgtgcg tcatttgcgc aactgtcgtt
    34801 ttagtttttt gactctagat cagttgagtc gtggaaaacg aaagatagaa aaagactttc
    34861 gtgaatagat tcagaaaata ttatgctaat ggtttaattt ttttttaagt tgtataaaag
    34921 aaaatgatta cataagagaa atgaaagcca agccagaaga ttcatagtag aataaaaagg
    34981 cgaatttttt aattataaaa aaaggtgtgt gttcactatg ggaaaattag tcgctgttgc
    35041 acagtcgctg cttgatatac tcaagtggct gatggaagga agcaattgga tatttgtgtt
    35101 gccactattg cttattagtc tatttatcat gtttcgcatg gtcagaaaaa aagaaacgtt
    35161 tctgattttt atctggtatg cagcatccgt tggggttgct accttgatgg ttgaattggc
    35221 gttaggtagt tgggaagctt gcgtatttgc tttatttttt gctgtactgt atctaatcat
    35281 ttatatccca tatatgaaaa gaaagtctcg gataggcaga aaaggtagac atacaactac
    35341 tttttctagt acagattctc tcgaacaaag tagaagaacg agaagaagac ggtaatattt
    35401 agtaggtata cgcaaaaaaa gataaaataa tggataaact attgagatga gagatttatt
    35461 taattggtgg aaaaaagctt ttttcactta tataaaagaa tcttttttaa aggaatgctt
    35521 gttgtatcgg gataagccat tccttttttt atgttattta tatatatata aatcatacca
    35581 atgttttttt gtattttctt gcttgcactt ggataagaga ttacttacaa taaattcaga
    35641 tagaaacagc tgaataaaaa aaggctccct gacccttagt tggcgctaag aatcagggaa
    35701 tgaccagtca cacacataca ccctgaccga tcattttgcc atagtcaatg cagtgcatcg
    35761 acctaattta ttgtacctaa tctagggtga aaattctacc cctatgtaga tattttccat
    35821 ggtctgcacg ttgttttatt tgctatgtca aggtattggg cgtattcgtt caatgccttt
    35881 tttgtgtttg aaacagcaaa gaccacatgt gttactgtct aactactgtt tttttatgta
    35941 cttccttaga ttaggcagct gtttctattc atgtcaagta tagctaggcg ggagagataa
    36001 atgagtttat gttattttga ctattatcac caattggaag agaaattaag gatagaagag
    36061 ccgctgatgc ttcaagctgg cacttggatc gcgaaacaaa ttaatcaagg tggtagattg
    36121 cagatatatg ctagccgcac actaagtggt gtagcatttg aattttggga tcaaaccccg
    36181 gatcttgtac caagtatatt gattgaaaat ccagccggag gtgtctatga aacgctagaa
    36241 ggtgcaggac aggcaattat tgatcagctt tctgtcaaat cagaagacat ttttcttctt
    36301 ctttcgaatg aaggaagaaa cccggcaatc atcgaactag ctcagtggat caaagaaaat
    36361 ggtcatccat taatcattgt tactggattt gatttgtcac gaagtatcaa atcgcaacat
    36421 agcagtggtt tgcgattatt tgaatttgct gatttggttt tggacaacca cgccaattta
    36481 catgatgcag tcttgacttt gccaaatcta gatccagcga tctgcggaac agcctctctt
    36541 gcaacgatct ttttgctgca gcaagttttg tattttacag caaaacagct agtccaggac
    36601 acaagtggaa aatagttatc cacatgttga caaagttcct taaaaaaata atgttccaca
    36661 taaaaatgga gcgtttgaaa aaaagaaaac gattattaat gaggattaac aacgactact
    36721 ttttttgttt tagttatttt aagatggatc atctttgatt ttaagcttgt tttgtttaaa
    36781 gtttgttatt tttataagtt gtttcacagt tatgagaaat gagttataag aatatgtagt
    36841 gtcagaaaaa taaaaagtag tggaaaaaga gacaagaaag aatccatatg gattctttct
    36901 tgtctcttaa tacacgttga tgaaagagtc ttccccaaaa ctctttacaa agtaattata
    36961 gtacatttgt tatcattatt gcaatgaagt ttacctgata taac
//
LOCUS       DAMEET010000017        36568 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.017, whole genome shotgun sequence.
ACCESSION   DAMEET010000017 DAMEET010000000
VERSION     DAMEET010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 36568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.017"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="PB780_000745"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="PB780_000745"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDE0732816.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="PB780_000746"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="PB780_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDE0732817.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="PB780_000747"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="PB780_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDE0732818.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="PB780_000748"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="PB780_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDE0732819.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="PB780_000749"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="PB780_000749"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDE0732820.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="PB780_000750"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="PB780_000750"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDE0732821.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="PB780_000751"
     CDS             complement(8053..8523)
                     /locus_tag="PB780_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HDE0732822.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="PB780_000752"
     CDS             complement(8569..8994)
                     /locus_tag="PB780_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDE0732823.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="PB780_000753"
     CDS             complement(9061..9330)
                     /locus_tag="PB780_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HDE0732824.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="PB780_000754"
     CDS             complement(9341..10936)
                     /locus_tag="PB780_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDE0732825.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="PB780_000755"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="PB780_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDE0732826.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="PB780_000756"
     CDS             complement(14489..15049)
                     /locus_tag="PB780_000756"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDE0732827.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="PB780_000757"
     CDS             15402..16376
                     /locus_tag="PB780_000757"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDE0732828.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="PB780_000758"
     CDS             16546..17946
                     /locus_tag="PB780_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HDE0732829.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="PB780_000759"
     CDS             complement(18024..18746)
                     /locus_tag="PB780_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDE0732830.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="PB780_000760"
     CDS             complement(18825..19331)
                     /locus_tag="PB780_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDE0732831.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="PB780_000761"
     CDS             19454..21253
                     /locus_tag="PB780_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HDE0732832.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="PB780_000762"
     CDS             complement(21321..22895)
                     /locus_tag="PB780_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDE0732833.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="PB780_000763"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="PB780_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDE0732834.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="PB780_000764"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="PB780_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDE0732835.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="PB780_000765"
     CDS             complement(24672..25310)
                     /locus_tag="PB780_000765"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDE0732836.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /locus_tag="PB780_000766"
     CDS             complement(25311..25961)
                     /locus_tag="PB780_000766"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDE0732837.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="PB780_000767"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="PB780_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDE0732838.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="PB780_000768"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="PB780_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HDE0732839.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="PB780_000769"
     CDS             complement(29104..29844)
                     /locus_tag="PB780_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HDE0732840.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="PB780_000770"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="PB780_000770"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDE0732841.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /locus_tag="PB780_000771"
     CDS             complement(31215..32153)
                     /locus_tag="PB780_000771"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDE0732842.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="PB780_000772"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="PB780_000772"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDE0732843.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="PB780_000773"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="PB780_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDE0732844.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /locus_tag="PB780_000774"
     CDS             complement(35313..35618)
                     /locus_tag="PB780_000774"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDE0732845.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="PB780_000775"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="PB780_000775"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDE0732846.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         9886 a         7988 c         6706 g        11988 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatcg cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaattg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gcaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ccagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 cgtcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc ttcttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc taattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgttcttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gatatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaata
//
LOCUS       DAMEET010000018        36422 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.018, whole genome shotgun sequence.
ACCESSION   DAMEET010000018 DAMEET010000000
VERSION     DAMEET010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 36422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.018"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(51..632)
                     /locus_tag="PB780_000776"
     CDS             complement(51..632)
                     /locus_tag="PB780_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HDE0732847.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(638..1897)
                     /locus_tag="PB780_000777"
     CDS             complement(638..1897)
                     /locus_tag="PB780_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDE0732848.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIAPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(1899..2945)
                     /locus_tag="PB780_000778"
     CDS             complement(1899..2945)
                     /locus_tag="PB780_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732849.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQRPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(3180..3455)
                     /locus_tag="PB780_000779"
     CDS             complement(3180..3455)
                     /locus_tag="PB780_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDE0732850.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(3698..5008)
                     /gene="der"
                     /locus_tag="PB780_000780"
     CDS             complement(3698..5008)
                     /gene="der"
                     /locus_tag="PB780_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDE0732851.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(5190..6419)
                     /gene="rpsA"
                     /locus_tag="PB780_000781"
     CDS             complement(5190..6419)
                     /gene="rpsA"
                     /locus_tag="PB780_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDE0732852.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(6554..7234)
                     /locus_tag="PB780_000782"
     CDS             complement(6554..7234)
                     /locus_tag="PB780_000782"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDE0732853.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAIQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(7283..7918)
                     /locus_tag="PB780_000783"
     CDS             complement(7283..7918)
                     /locus_tag="PB780_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDE0732854.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(7984..9387)
                     /locus_tag="PB780_000784"
     CDS             complement(7984..9387)
                     /locus_tag="PB780_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDE0732855.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGFLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVIHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKHQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(9374..10405)
                     /locus_tag="PB780_000785"
     CDS             complement(9374..10405)
                     /locus_tag="PB780_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDE0732856.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYERILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            10436..10657
                     /locus_tag="PB780_000786"
     CDS             10436..10657
                     /locus_tag="PB780_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDE0732857.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(10822..11559)
                     /locus_tag="PB780_000787"
     CDS             complement(10822..11559)
                     /locus_tag="PB780_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDE0732858.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGNSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(11713..13053)
                     /locus_tag="PB780_000788"
     CDS             complement(11713..13053)
                     /locus_tag="PB780_000788"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HDE0732859.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(13290..14813)
                     /locus_tag="PB780_000789"
     CDS             complement(13290..14813)
                     /locus_tag="PB780_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDE0732860.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(15191..15802)
                     /locus_tag="PB780_000790"
     CDS             complement(15191..15802)
                     /locus_tag="PB780_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDE0732861.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIP
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(15925..16049)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(16131..16847)
                     /locus_tag="PB780_000791"
     CDS             complement(16131..16847)
                     /locus_tag="PB780_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDE0732862.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(16853..17422)
                     /gene="scpB"
                     /locus_tag="PB780_000792"
     CDS             complement(16853..17422)
                     /gene="scpB"
                     /locus_tag="PB780_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDE0732863.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISFEEDVSPDLFFDGMNKGE"
     gene            complement(17406..18203)
                     /locus_tag="PB780_000793"
     CDS             complement(17406..18203)
                     /locus_tag="PB780_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDE0732864.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(18173..18544)
                     /locus_tag="PB780_000794"
     CDS             complement(18173..18544)
                     /locus_tag="PB780_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDE0732865.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGVYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(18563..19450)
                     /gene="xerD"
                     /locus_tag="PB780_000795"
     CDS             complement(18563..19450)
                     /gene="xerD"
                     /locus_tag="PB780_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDE0732866.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(19546..20019)
                     /locus_tag="PB780_000796"
     CDS             complement(19546..20019)
                     /locus_tag="PB780_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDE0732867.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(20124..20987)
                     /locus_tag="PB780_000797"
     CDS             complement(20124..20987)
                     /locus_tag="PB780_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDE0732868.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(21183..23324)
                     /locus_tag="PB780_000798"
     CDS             complement(21183..23324)
                     /locus_tag="PB780_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDE0732869.1"
                     /translation="MMKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDA
                     AMQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIE
                     KNEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHRED
                     TKEEHAKELLKMISEKIAQENKVEPEHEAEKDTEKDISGVNAAGKESLKTNDLKTNDL
                     KTDIPEKTETLSEKQSEDEKSNGLPEADKKEMLEKADKKATESDGINNEADAETQSAS
                     EDSTALEAKIEKEENQPELIEAETEPITIVPNTEKTPQESNPEKKDLDKTQKKKSKKV
                     PYLALAAVLLLGGGGWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFI
                     RADMIHQDLSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDH
                     LADDIKLKADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVV
                     DSATRDQYKTAKEAVEKVKNTDLSAQLNEQLQQVDKMLTDKEKKEEEEKKKAEEAKKA
                     EKAKKAAEEQAKQAAQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARAS
                     DIADASNPAWTWAPGVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKS
                     ALMKGIGESALPFYIVTINCKTGWFGGNGSN"
     gene            complement(23541..24650)
                     /gene="rpoD"
                     /locus_tag="PB780_000799"
     CDS             complement(23541..24650)
                     /gene="rpoD"
                     /locus_tag="PB780_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDE0732870.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(24674..26515)
                     /locus_tag="PB780_000800"
     CDS             complement(24674..26515)
                     /locus_tag="PB780_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDE0732871.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(26697..29012)
                     /locus_tag="PB780_000801"
     CDS             complement(26697..29012)
                     /locus_tag="PB780_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDE0732872.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(29197..30057)
                     /locus_tag="PB780_000802"
     CDS             complement(29197..30057)
                     /locus_tag="PB780_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HDE0732873.1"
                     /translation="MEKRQKMENQKVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(30116..30748)
                     /locus_tag="PB780_000803"
     CDS             complement(30116..30748)
                     /locus_tag="PB780_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HDE0732874.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMRSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            30915..31676
                     /locus_tag="PB780_000804"
     CDS             30915..31676
                     /locus_tag="PB780_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDE0732875.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            31702..34293
                     /gene="mprF"
                     /locus_tag="PB780_000805"
     CDS             31702..34293
                     /gene="mprF"
                     /locus_tag="PB780_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDE0732876.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLVSVLPMIGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDP
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(34481..35668)
                     /gene="tuf"
                     /locus_tag="PB780_000806"
     CDS             complement(34481..35668)
                     /gene="tuf"
                     /locus_tag="PB780_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDE0732877.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            36036..36392
                     /locus_tag="PB780_000807"
     CDS             36036..36392
                     /locus_tag="PB780_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDE0732878.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT        10119 a         7708 c         6251 g        12344 t
ORIGIN      
        1 attccacatc cagctgatct ttgtcttttt tagtactata ctatttttaa ctaattgtta
       61 tgttcactta cccggttact tttcttttgt gtctgcaaaa ggatctttcg gttaacttca
      121 tcagataatt tcaaaaaagc atccctatct ccatcttcta gtgcttgatc gatttgagca
      181 taaagcattt ttagttttgc ctcttcttct ttttcttgaa aatatcgttc aattaattca
      241 gtatcttctt cactgactga agaatcccaa gaagaaaatg gattgtcttc taagatagat
      301 agatattctc ttgtctgcca agcattttcg aatatacatt ccacatataa aggctttttc
      361 caattcaaac ggatttcatg aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa
      421 aacaatttca tgggttcccc ttcaacagat agatcactga tctgcagtcc tcgatccgca
      481 aaattcgccc cttcaacaaa gtggacattg cttaaaatcg cttcatgatt ggcaagataa
      541 ttcaagatcc aaaaaacttc tcttcggcta aaagatacat gattgaccat ccacgttaaa
      601 aaattcttct tttcccgtac atcaattagc atttttctca cctctctgtt aggtcatcta
      661 ataatgacag tgcctccata tctcctggtt caagtgttaa ataataagtc aataattctt
      721 gcgctctttt cagctgccct tcttctctta agaaaaaggc atactctttc aagaaatctg
      781 gttcatgtga tagttcatga taagcctgtt catagtgtac agctgcaacg gtaaagtctt
      841 ctaattcatt ataagcatgg gctaggttcc attctgcata aggattagca gtttcttcca
      901 tctgattgat cacttgtatc acatcttcat atctttcatc atccaagtaa agattgctta
      961 aagtaagtaa tgtttcatct tgtttgtctc ctacttctaa agctttcagt aaaagttctt
     1021 ctgctcgctg tctatcatgc agacggaaag cattttccga ggccaggtga taaagttcga
     1081 cttggtaagg attttctttg attccttctt ctaacactgt ttgtgcttct tcaatcattt
     1141 cttcttcttg aagagcttgt cctagatata gatataacga ttgataatgc ggattaactt
     1201 ctcgaagttc ttccagatag ttgattgcct gctgattttc tttcaattga agattcgtaa
     1261 aagctagctg gaacagtcga tcatctgtcc tttcttcttt cagtgcttct tctaaaggtg
     1321 cgatagctgc ttcgaattct ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg
     1381 agattcctga aatttcattt atcccgcttg ccaacaatgt ttcataaacc actgctgcct
     1441 ctttgaaacg atcaactgaa aaatacaatt cagctagcgc aaactggatc aatggttcat
     1501 caggaagcaa cccagctgct tctttcaatt ttgcttcact cacttcaggg attccgataa
     1561 cttggtacaa atcagctatc gccaataagc tttgcggata gtaatcactt tgcttatcca
     1621 ctttttccag gtattcaaaa gctaagtcga tctcattatc ttcgattgca atttctgcta
     1681 acgggatgtt cagtccgtca ttttctgggt attggttgat cagttgttca aaaatctgtt
     1741 ttgcttcgat caaaaagcca atagccagca attcttcgcc tagttcagct aatatatctg
     1801 ctgggtcttc ttttaatgct ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg
     1861 ataaagcttg caacattttt tcgctatttg tattcatttc aattccctct tatctcactg
     1921 acgatttgtt gaaataccgt cattgttctc aataattctt tttcgtttgg acgttggtat
     1981 ccgtctatta tacctatttt ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa
     2041 agaacttttt ccaaatactc catctttgaa atctgttcca gccagtggat tggatagcca
     2101 agcaagtcca gccacacaaa aaagtttttt agttggaaat caaaaggata tgcttggaga
     2161 ttccacacga aatgaaacat cattcctaaa aattttttca tactagcaga aagtagatgg
     2221 ccattttcgg tcagataaaa agcttgttca aacagtttac cataatttga caggttttct
     2281 gcctctaatt gataaaaata gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt
     2341 aattgctttt tatttggata gtttttatac agattttgca aaaaacgatg atcacaaatc
     2401 attccgcaac agataaaagt caaaaagtca atctgctttc cgtccacttg ccgctcgctg
     2461 attgtttgat cataaacgat gcgttctggc agattgatgg tttgcagtac tacacgattt
     2521 ggtagctgca aaatcgtccg ctgcggtgtt aatgctcggc ttagagaacg gatcgataca
     2581 gggatcatcc atagtgatga atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc
     2641 atgatccctt catttccaaa tccgatgata aacatggatt gattttcggg atagcgtttg
     2701 acgaaatcaa gcaggttctt taattctgtt aaatgattgc attgagtgtt ggcacaaata
     2761 taccagtcat tgtttgcttg atttgcaaat aacgggttga tcttatctgc aaacaaatca
     2821 taatagcgct gattcgttaa aaataagatt ggctgatcaa tggaaagttc tcttagctgg
     2881 ctgactagcg tttctccgta aaacactttc gtcaacgcat cttctacttg ataatcaagt
     2941 tccatacgca tccctcctct tcttattttt attttaccat tagtaagcaa attctagaaa
     3001 agataagagc aaagctcacc tcataacttg gaaataaata aaaattaaat aaaatacatg
     3061 tgttttctat gtatgacata cggattcgga atctctgtct cttatttatt ccttttctgc
     3121 taggaaataa aaagaaaagc acttgtagcc ctagagccac aagcgctttt tcaactgtct
     3181 tatttaacag cgtcttttaa tgctttacct ggtttgaatg caggtacttt gctagctgga
     3241 atttcgattt cttcaccagt ttgtgggttg cgacctttgc gagctgcgcg ttcgcgaact
     3301 tcaaagttac caaaaccgat taattgaact ttttcgccgt tagctaaagc atcttgaata
     3361 gttgaaaata cagcatctac tgctgcagtt gcatcttttt tagttaaatc agtagctgaa
     3421 gcaacttttt cgattaattc tgctttgttt gccatggata gattcacctc ctccacaaga
     3481 gatatgatag aaatatcatt tagttaaaac tttctgagtg gtccagaagt ttaaaaagaa
     3541 atattgatta acttcaatat tctgttatca cgatatcata tgaaccgctt aatagcaagg
     3601 tttttaccaa ttttcacatg ctttttttag tcatagaaac gtaacaaacg aaactgtctt
     3661 tttcttttga aaaaaaaacg tcgatatggt aaaatgccta ttttctccta cgagggataa
     3721 ttcggatgga cgtcccttca aaagtaaatg cttttctgat ttgattttct aagaaacgtt
     3781 cataagaaaa atgcatcaat tcctcttcat tgacaaaaac gacaaatgta ggtggtttga
     3841 cagcgacttg tgtagcataa aaaatcttca ggcgttttcc tttatctgtt ggtgttggat
     3901 tgattgctac agcatccata atgatatcat tcaacacagc cgaagagata cgaagattct
     3961 ggttcatact tacttgttcg atcatttcag gcagacgttc caaccgttgt ttagtcactg
     4021 cagaaacaaa aacgattgga gcgtaatcaa gataccggaa ttcatcacga atctcttgtt
     4081 cgaaatcacg cattgtattg gtttctttct ttacaagatc ccatttattc acgacgataa
     4141 taattccacg accagcttca tgggcgtaac ccgctactcg cttgtcttgt tcccgaatcc
     4201 cttcttcagc attcagtacc atcaaaacga tatcagaacg gtcaatggca cgcattgcac
     4261 gcatcacgct gtatttttca gtattttcgt atacttttcc gcgtttacgc ataccagctg
     4321 tatcgatcat taagaatttc tgaccacttt cagaaacgaa gtgggtgtcg attgcgtctc
     4381 gggtcgttcc ttcaatatcc gaaacaatta cacgttcttc tcctaaaatc gcattgatca
     4441 aagaggattt accaacattt gggcgaccaa tcaaactaaa cttaatcgtg tcgtcgtcct
     4501 cttcttcagt ctcttcagag aaatacttca ctgcttcatc taaaatatca ccaatcccta
     4561 gtccatggct tcctgaaaca ggataaggat cgcctagccc taaggaataa aattcataga
     4621 tatcgtttct catttctgga ttatctactt tattgactgc taaaatcact ggtttattac
     4681 ttctgtataa aattttcgca accatctcat ctgcgtctgt cacgccttcg cgtccgctga
     4741 ctacacaaat gattacatct gcttcttcaa tcgcgatttc tgcttgatgc ttgatctgat
     4801 ccataaaggg ttcatcgcca agatcaattc cgcctgtgtc gatcacgctg aattctcgac
     4861 ctaaccattc accttttgca taaatacgat cacgagttac tccaggagta tcttcgacaa
     4921 tcgagatacg ctcaccagcg atgcggttaa aaatcgtaga cttaccgacg ttcgggcggc
     4981 caacgattgc aattgttgga tttgccatat tatttcctcc tcacaagttt ccaatctcac
     5041 ttagtttaac gaatgcattg ccaactttca agcatagatt gtcaattaaa cgaagtattt
     5101 ttattcctta ttgaaatact aatcgctttt caaaagaaaa actgagacaa aagcagctgc
     5161 tttcatctca gtcacagata caaaatcaat tatttttcag aatcatcggt tgctttttct
     5221 tctacttctt ctttcaacgc atcacctaaa atgtctccca tagtaaatcc agtattttct
     5281 tctggtaatt cataagactc aacatttttt ggttcttctt ctgaagctgg tttttcttct
     5341 aaagctttga tactcaatgc aatacgatgt tcttctggat ggatctctaa tactttcact
     5401 ttcacttcgt caccttcatg aagcacttca tgtggtgtcg caatatgttt atgtgaaatt
     5461 tgagagatat ggactagccc ttctactcca gggaacactt caacaaaggc accgaagctt
     5521 gtcaaacgtt taactgttcc gtctaatact gtgccaactg gagcttttgt atcgatttct
     5581 gaccaaggtc caggctgtgt gtctttgatg gacaaggaaa cacgttcttg ttcaggattg
     5641 atagacaaca ctttgacatt tacatcatca ttgacagcca atacatcact tggttttgct
     5701 acatgactat gtgaaatctc tgagacatgg actagaccgt caattccgcc taggtcgaca
     5761 aatgcgccaa aatcagtaag acgtgcaaca cgtccactga gaacatctcc ctcatggata
     5821 gttgaaagaa tttctttttt cttttcttct ctttcgtttt ccactaccgc tttatgcgat
     5881 aagatcaaac gattttctga tggttcgatt tcgatgattt taaagctcaa tgttttgcct
     5941 ttatagtctg aaaagtctgc aacaaaatga tcttccacca ttgatgcagg gacaaaacca
     6001 cgtacaccga cgtcaacgac taaaccgcct ttgacaacat ttgtaacagg tgcttcgatc
     6061 accttgccct cttggaaatc tctttcgata tcttcccaaa cttttttagc atctaaacgg
     6121 cgttttgaaa gtaggtagct gccgttttct ttatctttgc cgatcgtact gataacgact
     6181 aagtctaata catcaccgac ttttactact tcgctgatat cttctaccgg caaagttgat
     6241 aattcttttg ccggaacgac tccttcaact ccagcccctt cgattccgac aatcacttgt
     6301 ttgtcttcaa tgactaaaac ttcgccctta acaacatcgc caacttttac ttcttgtacg
     6361 cttttcattg cgtcttccat tgactggttg ttctcttgat tttgatcaaa ttctgtcata
     6421 acacttgtcc tcctaaccaa cattctgccg taagtacggt actgttttta gtttaattga
     6481 ttattcaaaa aaaataaagc gatttcgttc gttttccttg aaaaaacaat tgtttttcat
     6541 tttttttctt aatttaaaaa agaagataac cttttttcaa aacaacagct tttattgctg
     6601 caacgacttc tggaatcgat ttgccagtcg tatcgatacg aacagcatcc tctgcttgct
     6661 tcagcggaga gacgtccctt gttgaatcat aatggtctct ttcctgtatc tctttcgtca
     6721 gtgtttcaag atcagtcgga attccttttt cttggttctc tttgaaacgt ctttctgcac
     6781 gctcacttac gcttgctact aaaaagattt tcacttctgc attcggtaat acagctgtcc
     6841 cgatgtctct tccgtccatg acaacgccac cgcgttcccc tatttttcgt tggatagcaa
     6901 ctaattcttc tctgactttt ccatgtgcag agacttcaga aacagctttt gtgacatctg
     6961 gttgacggat ttctaaagtc acatcttttg atgctatgaa tacatgctgt ccatcttctt
     7021 gttgcgaaaa agagatagga tactttttga tcagcgaaac tagcccttct tcatctgaaa
     7081 aggatacatt gttcgtaata gctaaataag ttactgcgcg atacatggca ccggtatctg
     7141 tataaatata attcaattct tttgctaata tcttggcaac ggtacttttc ccagatgaag
     7201 caggcccgtc gattgcaata ctgatttgtg tcatgatata actccttttt ctcaaaaaaa
     7261 aagccgcaaa gcgcggctat tattatttaa ttcttaattc ttgaccaggg taaagcataa
     7321 agttgtttgg atctaagcca ttcaattcaa acaattgatc agtggtgatc cctgcacgct
     7381 cagccacttg ttttggacct tcgcctgctt gaacagtcgt tgttgaaccg gctgcagctg
     7441 atgaagaagg tgttgtttct gctgcattgc tgcttgaatc ccctgcttga taagttgcct
     7501 cacttgagga agtcggtgtt gtctctgctg caatactgct ttctgacgtt acacctgtat
     7561 cttgagacac agtagattct tccgcgattg tactagattc tactgttgaa gtctcagttg
     7621 actcctcttt tgttgtactt gtagaacttt ctactactga tgtcgaagtt tcagaagaag
     7681 ttgctgctgt tgcattgttg cttgatccat ttctgatcca gaaataagca cctgctggaa
     7741 tagcgataca taagaccagt aaaatcaaaa atagcgaaag aaataatgta tttcctttct
     7801 tttgttgtct ctgagaactt cttgatgaaa cttcatcatc tgtatcgtag attggttgtt
     7861 cccatggttc ttgtgtttcg ttattttctg tagattgatg tctatctttt ctgctcactg
     7921 ttttacctcc taaactctat cgtgtgtatt ataccatagg aaaaagtgtc ttgtcctaaa
     7981 aaatcaatgt tccatgtttt ttttaaataa atttagtaaa atcgtttccc aatgaagttg
     8041 ttcagtctta gaaaaaagct tcctctttgg cagaagttct gcttttgctc catcaatatc
     8101 acaacaattc tgcggctttt cggagagttt ttcgccaaaa taagctaaga tgaattttct
     8161 gcggcagttt tcttcattaa tatagcgaag catctgctgc aggcggaatt ctttttcttt
     8221 ttcctgatgt ttgagccgtt ccacccaatt ttctggtttt tcagactgct ggatcagctc
     8281 catccatttt ttttgcagct catcaaaggg agcttgctct gtggcatatt ccaaataaag
     8341 ctcaaaactt tgtcgctgtt ctctagaaag ttgattaaag aaataatgga tacgttcatc
     8401 cccactttga taaaaaagaa ttgctgcact ttcttcctga tctctgcctg ctcgaccgat
     8461 ttcttgtaca tagttttcta gactgtctgg caaatcataa tggatcacat aacggatatc
     8521 agatttatcg atccccatac caaatgcatt cgtagcaatg agaaactgca gctgattttt
     8581 tacaaactgc tgttgaagtt gtcttctttg agctgcatca agaccaccgt gatagtatcc
     8641 aactgtaaaa cgttccctga ataaatgata caatcgttcg acttcttttt ttgttgcaca
     8701 ataaatgatt gccgctcctt gtgctctttc catgaagtgt tcaagttctt gttctttttg
     8761 ctgcgttttc cgaacaaaaa gcgagatatt ttgccgattg actgattgtc tgatctcttt
     8821 tggtggtcga tcaaatagta catgacgtat atctcttgct actaaatctg ttgctgtcgc
     8881 cgttagtgcc agtgtgaccg ggtttcctaa aagctttcgg atttttccta actgttgata
     8941 ttctggacgg aaatcaactc cccattgaga cacgcaatga gcctcatcta ctacatataa
     9001 cgcaatattt tgttttttca gatgctctaa aagagaagga ttagtcaaca tttcgggact
     9061 caaaaacaaa aatttatatt gcgatagatg acggaggacg tatcttcttt cagattgcga
     9121 aagtaaacta ttgaaagcag ctactcgttt ctcccctcgt ttttgtaatt gcgtcacttg
     9181 atcttccatt agagaaagca gcggtgacac gatgatgacc agaccttcca ttaaaaaccc
     9241 cgtcaattga tagcaaaggc tttttcccgt tcctgtaggc aaaatagcga gagtatcttc
     9301 tccatcaagc aacgcctgaa tgatttcttc ttggccggga cgaaatgaat caaatccaaa
     9361 atattttttt aattcattta ttaacattct gtatcgcttc ctttctcaat agatagattt
     9421 gatacaaacg aaattctcca taatctaatt gtccatctac attccattcg ctaaagcgcc
     9481 actctcgcac ggaatcctta tttggttcca aacgttcgat tgtttctgca gagagaatcc
     9541 gctcgtatgg aaaatcatca aaaaacaagg cccactcaat aagatgatct gttactgttc
     9601 ctctttttaa atgacggatt gccataatct ggtcaattgt ctgaccattc ataaacatct
     9661 gcttggtgat catcatgctt tgattgaaat tttgctgtaa taatgaatcg atcaaagcgt
     9721 gccaaagcga atctggccgt ttttcgattt gtgctaacaa aaggtggata catctgcttt
     9781 gttgcagata aaccgctgac tcatcattgt tttttgccag ctgataagga agcagccctg
     9841 ttcgctcatt tccagataat tggttagcta aaaaatctgc tgcttcttct ggtatctccg
     9901 aaaacagctg caccaaactc tcatacgcag tatcaatcaa aatttcacgt ggcaattggc
     9961 tttcactaag ccattttttt agttggaaag tataaaaagg acttgtctct gctggaagat
    10021 agtcttgaat acccgttgcc agattagaaa ttacttgaac agcaaattta atgagccgcc
    10081 aactagtttc ccctgttctg ccatagcggt caaaacgcaa accagtatgt tctatttgat
    10141 gttcggacaa catatcagcc cctgcaggcg ttagctttgc ttcattttca tttatttcga
    10201 tccaaccttg ctgcgcaatt ttttgcaaaa tttgataaaa cttatcctgc ttcaattctg
    10261 gaaaactgtt atggataaac aaaagttcgt gggtaaatcc ataaataagt actgaagtcg
    10321 ttcgttttcc cacaaggagt tggtaaagag aactaatccg cagcttgtgt cctgatgaaa
    10381 atagcgataa aataaaaaca atcatattat tctccttaag ttaggatggt tagaaatgtc
    10441 actactatgt aaacttgtac ctgaacgctg tattgcatgc ggtctttgtc aaatcgaagc
    10501 gccagatatt ttcgattatg atgaagaagg aatcgtttta tttgcccaag aaccagaggc
    10561 tcatcaacag ttcgtgatgc cggaagaaga aacatatgta aaaaaagcgt atcaaaaatg
    10621 tccagttcga gcgattttat tagaaaataa aatataaata taaacgaaac tttaaaaacg
    10681 tacactaagt tctctaaact tgaaaataag ccgggatctg tttctcttgt tccgtgaaat
    10741 cccgacttat tttgaaagat tcatttgcta tctatagtta attgactagt tttttcacgg
    10801 accctatagg acccatttat attatttcaa ctttttcagc cagttttcca atgtattgac
    10861 gatcacagtt tgctggtctg cgttagaaat ttctgcctct ccatctcctt tttgctcacc
    10921 atagctgcca aaacctccat gatttccgcc attgattgat acatactctg ttctttccgg
    10981 taaatactgc ttagcttctt gataagcttc ctgattcaat actttatctt ttgtcgccgt
    11041 gatcgataaa acaggaacag ctgattctgt caatgttcct tttttgtcgg gataacttgc
    11101 catgaagaat acccctttta aatgtttgtc tgaatgattc ttattgacat aacgacttgc
    11161 cattacgcca cctaatgaat ggccaccgat cacataatcg gaatttggaa aatcttcttt
    11221 gaccttatcc gctttgtttc cgcccatcac tgctagatca agtggcattt tgacaatagc
    11281 gactgaaaag ccgttttttg ctaaatcttg cgcccaaata ctataacttt ctggctctac
    11341 gagagcacca ggataaaaaa taagtaatgg agaatttcca tgattctttt tctctccttc
    11401 aaaaaataag taatcttctt tgttttttcc ttcttttacc aacacttgcg cttctgacga
    11461 tggttcgtac gtattttgct gtatatagta ataaccggct ccaccgagta atcctaaaat
    11521 gatgactaag ccaattagta ttcgtctaaa tattttcact ttctcccacc ttttctatct
    11581 gagtgacccc tttttcttaa tcaagtatga ttatacctta aaaaaaataa gagagaaatg
    11641 caaactttcc agcatcaaat aaaaaaagac ggaatatcca tttgaatatc cgcccttttt
    11701 gtatgtttat ttttatttag cacataaacg atacacagct tctgcataaa tttccattgc
    11761 tttatacata ctacttaatt cccatcgttc attcgcctgg tgcataaagt caggagtatc
    11821 agggaacatc gcaccgaatg ccacacactg gttcatcgta cgagcaaaag tagcgccgcc
    11881 tgatacaaac ggttcagtca tgtctccagt aatatcgcga tatgtcccca atagattttt
    11941 cactaactca ctgtctagtg gcacgtataa tgaatcaagg aagtcaaatt cttcataagt
    12001 caacccatat ttttctgctg ttttagttaa tttagctact aaatcatctt tcttgaatgt
    12061 aacagggatt ctcatatcta caccgatttt tgtttgttct ggtgtgattt ccaaacttgc
    12121 aaagttcatc gttagctcgc cggattgttc atcttctgtt ttgcccacga cagcttcacc
    12181 agttgcattt tcttgaacta attttcctaa gaaattgatt gggccaaaat cgaatacttc
    12241 tgaaagagca attgccagac gagaaatagc attcactcct tgtgctgcat ctttcgcgtg
    12301 gatactttta ccaagaacga cgatgccttc accttgttct tcgaaatcga attcgtgttt
    12361 tttcaacgct tcttttactt cagaaagctt ttctccagag taaggagctg catcaggaac
    12421 aacattcaac gctcctccag cttttacaga aaactcgttc gttcctggtc ctgtcaagta
    12481 agcttgcaat aagccttttt ctgcataaat taaaggaaat tcagcatctg gcgcaaatcc
    12541 ttgcgtaatt ccttcttctt tttcgttata tttttctaaa caacgccata aattttcttc
    12601 atctgtacca aagataaaac gaatacgtgt cttgaattcc acgccagcat caatcaaagc
    12661 tttgacggca tacattgccg caatagaagg acctttgtca tcttgggatc cgcgaccaac
    12721 taaccagccg tcttttatag ttggatcgaa tggttttgtt tcccaattat tttcgtcccc
    12781 tgctggaaca acgtccatat gacacaagat accgaataat tcatctccag agccaatctc
    12841 tgaataaccg taatagcctt ctggatcttt aaatgttcta aatcctagct tttcactgat
    12901 ttccaatact ttatccaaag cttcaatcac ttttttaccg aagggatgtc cttgtcctga
    12961 atctgcttca tctagtacgg aaggaattcg gatcaattcg ctcaatgatt ctaatgcttg
    13021 ttcatgatgt tgttctgtca caaatttttt catgatatta tcctcctaat atgttaaaaa
    13081 gggtcatgtc aaattcctcc gacacaacct cttcttctca aataatgacg ttttaagcct
    13141 gctccatcag caagcagaat gttcttaaca aatgatgaaa gaaaaaacac tcactgaatg
    13201 gagctattga cttgtccacg tgcttatcga tagccgttta tcggtttctt cattcttatg
    13261 atagaccttg tacctgtatt tgtctaggtt tagaacattg ctgctacacc taggatcaca
    13321 cagatggcta caaataatat cgcaattaat ttaccggtaa atttccacca cactgaaaca
    13381 tcgaaacgag caattgctac agcacccatt acgatagcag atgttggtgt aattaagttt
    13441 actaaaccag atgcagattg ataagcagtg attactaaat ccttaccgac gccggcaaat
    13501 tcacccatag gtcccatgat tcccattgtt gcagcagcta agccggaagt tgaaggaatc
    13561 aagaatgaca tagggatata gaaaataaat gtaataatga tgaatacact tgaagataat
    13621 gaacttaatc cttgttcgcc ccaatgtaag atggtgtctg tgattaatcc attgttcata
    13681 accacttgga taccacgagc aacagctact acgattgcta caccgatcaa atcagaagca
    13741 ccagctaaaa attcagagac aaattcagac tctttcatgc cataagtgat tgctactacg
    13801 atggacatta ctaagaataa catagtaatt tctgggaagt accagtcacc taatggaagc
    13861 atgcttttac ctagaacagt acctagtacc ggaaggtttg ttaaccagtt tgtcaatgat
    13921 tcaaaaatcg tgaaattcgc attcaaggtt gtccaaggaa tcaaactgat gatcatgata
    13981 ccaaaagtaa taaagaacaa tacattaaca tgtttttgtt ttttcgtgat tacttcttga
    14041 cgaccgattg cttcaatatc gaaatgcttc atatcttctt tacgttggtt gtatactaat
    14101 gattttgttg gatctttttc gatctttgat gcataacgat acacatatac aatagaaata
    14161 gctaacaaaa taacgaacat caagaatcga agaccaatac cttcacccat cgaaatccca
    14221 acagcttgtg aagctacgcc tgttgcaaaa ggatttaccg ttgaagctag acagcctact
    14281 tgagacccta ctaatacgat tgctactgct actactgtat caaagcctac agccatcata
    14341 actgggatca gtaaagcata gaatggaatc gtttcttctg ccattccgta agtagagccg
    14401 cctaatgcaa ataacagcat caatactgga ataagcattt tttcttttcc tttgaatttc
    14461 ttcacaactg aagcaatccc ggcatctagt gcccctgttt tgttgataat acctaaaaat
    14521 ccaccaacaa ccaaaatgaa gaaagagatt gggattgcag caggtgtttc tttagttagc
    14581 tcccccgctg gtacacctaa catcccattg attggcgcca tgaaaatatc ccacagccct
    14641 tgaggatttt gttgtactcg ttcatatgat ccagcgacga tgttgcctgc atcgtctaca
    14701 ctatagtgcc cagctggtat aaaccaagta agtacagcga ttactgcaat aataataaat
    14761 aagaccgtat acgctgaagg catttgaaat tttttctttt ttgtttgttc catcttcttc
    14821 ttcctttctt ttccgttaga gacaacaatt ttggctctaa tgttatttta tcaaaaagag
    14881 tatcagaaca aaaataataa cacgcatttt gtaaaacttt tgttcaaaaa gttcgttttt
    14941 ccctattccc tcctcttttt tcaggttttt aaagctttct tctttttcat tgtagcgttt
    15001 cctttttttt ataaacaaac attttcgtct tttaaaagga gaagaatttc acatttgcaa
    15061 agaattgatt gatttttatg cataaatcaa aaaaatactc aaaacaaaca aataaaacgt
    15121 ttacattatt gcatgtcttg tatttttttc caaaaaaaaa gaccaagaaa tttcttgatc
    15181 tttttgataa ttatttgact gctgtatgac gttgttcgaa ctgatgttgt tttcgactta
    15241 accatggaag aagttgcgca tgcagcacta aaaagacagc gctcacgatg aatcctttga
    15301 tcaagttgaa aggaacgatc ccaatcagta catagtttgc taaagacatg cctagcatct
    15361 gtgttgcgtt cagtcctaaa aacattaagt aaagaggcgt aataacgaaa taatttgcga
    15421 tactcataaa aatagttaga gcgatggttc ctgtaatgac acctaaaaat ttgtttcttc
    15481 ctttatgctg tttctttaca ctaaagaaat aataaatcgg gaatgtaaag atcgttgttg
    15541 ataagaaact agcagtgtct cctaccaaat tatccggaga aaaacctgta gtcagcagat
    15601 gtaatactga acgcaagaac gctgttgcaa ctccggccgc tggaccgaat aagaacatgc
    15661 tgatcaatac cgggatatca ctaaaatcga tcttcaaaaa actaaaagcc ggcatgatcg
    15721 gaaaagcaaa atactgtaaa actaacgcga tcgcagcaaa catcgctact gcaaccattt
    15781 tctgtacacg ggtgttcttc atttgaaatc ctcctgttag gactcctctt cagcgaactt
    15841 tttgacgttt ctctccaaat gaaaaaactc tattttccag ggaaaataga gttagagtta
    15901 tttgatcaag cgtcgtcaaa taagctctat cttctttatc cagactatac tgtcggtatc
    15961 ggaatttcac cgattcagct attttttgca aaataggtcg tggactataa ccaccggtcg
    16021 ggaatttcac cctgcccctg aagacgaacc ttatataatt tttgtttaca tattcattat
    16081 acatatctat tcaaaaaacg caacatctaa cacttataaa aagtaagtgt tcattttgag
    16141 tggttcagca aactgctgac ttcttttttg ttcagttcac ggtaatctcc aggacgtagg
    16201 ccttgtaaag tcagatctcc gtatttttca cgttttagtt tttgcacagg aagaccaact
    16261 gcttgaagca tatttttcac ttgatgatta cgtccttcgt ggatcgtcaa ttccacgata
    16321 ctgtggtttg tcttaggatc cactgataaa atttggaatt tagctggaga tgtttttcgt
    16381 ccttcgatct ttattccttt tgtcaaaggt gcaagcatcg attttgtagc gatccctttt
    16441 acctttgcaa cgtaaacttt atccacttca tgttttggat gtgtcaggcg ctgagcaaaa
    16501 tctccatcat ttgtcagtag caagatcccc gaagtatcgt aatccagtcg acctacagga
    16561 tagatacgtt cttttaccat cggcaaaaga tccgtaacaa cttttcttcc tttgtcatct
    16621 gcaacagcag aaataactcc tttaggtttg tacaataaat aataaccgta ttcttcttga
    16681 taaattggga cttcatccac ttccacagtg tcatgtttcc caacttttat ccctaattcc
    16741 cttacaactt gtccgtttac ttttacacgt cctgcaagga tgaattcttc tgcttttcga
    16801 cgagaagtga tgccagcgtg ggcaattact ttctgcaatc tttccattac aattattctc
    16861 ctttattcat tccatcaaag aatagatctg gtgacacgtc ttcttcaaat gaaatttcca
    16921 tttcttgtgt atcaggcagt tcttctaatg atttcaaacc aaaataatct aagaagtagc
    16981 cagttgttcc atataatatc ggccgacctg gcccttctac tctacctttt tcttcaatca
    17041 gtttatgggc agctagacgc tgtaccgctc ctgaaacctg aactcctcga atctgctcga
    17101 tttcagctct tgtgattggc tgtttataag cgatgattgc taaggtttcg acagcggctt
    17161 gagataatgt gtttgatgtc ccttgcgcat atcgcttaag taagggagcc agctcttttt
    17221 tagtcgttaa tacataatgg ctttccgttt caaaaatatg caaagctgag tgcgagtttt
    17281 gagtgtaaga ttcttttaat tctttcacta aacttgtgac ttctgcttcg ctgagattta
    17341 acagtccgct taattcttca ttactgatcc cttcctcgcc aacaataaaa agaagggctt
    17401 caagttcact taatttcgtc atgttcggta tctcctactg ctggataaag cataattgat
    17461 tcatagtttt ctttttgttg cgcaatgatc tgcccttttt tcatcaattc tagcaaagcc
    17521 ataaaagtag tgacaacttc acttttagaa ggtgactcaa aaaaagaatc gaaagtacag
    17581 ccatgcgcat ttctttttat acgctcttcg ataaatacca ttcgttcttc gatcgttgtg
    17641 tcatcgggcg tgatcgtttt ttccactttt tgagaaaatt tcttacgttc taagattttc
    17701 tgcatggcac ggatcaaatc gatttttttg acttgtcctc gcttcaacgg cttttcagca
    17761 tccgctaacc catcgatgtt cataggttct tttgtgtaat aaagacttcg ttcctctgct
    17821 ttttctgtca gtctttcagc tgcatattta tatttacggt attctaaaag ctgagtgacc
    17881 aattcttctc gaggatcttc ttcccataat tcttcatcat ctgtgacgaa ttcttggaca
    17941 ggaagcaatg tcttgctttt gattgccatc aatgtagcag ccatcactaa gtattctcct
    18001 gcaacagcta gctctagtgt tttcattgta tggatataat tcatatattg ctccgtgatc
    18061 gtagcgatcg gtatatcata tatatcgatt tccatttgct gaatcagatg aagcagcaga
    18121 tcgagaggac cttcgaatac atctaattta atattgatct ccgtcaactc attcactctt
    18181 tcttcttttt ctttgctagc cattttccaa tgtaaggctc tgtgatttca gttgcacgga
    18241 tggagacatc cgggtacttt tcctgtaatt catctaacat ttgaaagccc atattctcac
    18301 cgcgataaga cggattcaaa gaaatgtcat ggatgatcag ctgatcgttc gttgcccagt
    18361 aaacaccaat aatcccttga atgttggttg aaccttctgg cgtaaagaaa aaaagttcat
    18421 aatcgtcttc agattcgtac gtatcaatct ccttcaacaa agaagtttta cctgtcagtt
    18481 tcttatggaa actcaatagt cccatagcaa ttttacggtt ttctttccgg taatgtgtta
    18541 acatcttctc accttcctat gactaagccc ttggaaaatg ctgcttataa acatccgtca
    18601 ttcttttctt cgtgatatgt gtataaatct gagtcgtaga gatatccgcg tgacctaaca
    18661 attcctgtac tgtccgcaaa tcagctccat tttctagcag atgtgtggca aagctatgcc
    18721 gcagcgtatg tggtgttact tctttgtaaa tcccagcctc tcgtactagc tgcttcagat
    18781 ttttccagat tccttgccga gaaagacctt tcccatgatt attgacaaat acatgcattt
    18841 cagatgcgtc ctttacaagt aatggacgtg cttcgtctaa gtagcgttcc agccattgga
    18901 tcgcataatc acctaaagga atgatccgct ctttatctcc ttttccaact gtctgtacca
    18961 gtccaagaga aagatgaaga tctcccaatt tcaatccaat caattcactg acgcgcatcc
    19021 ctgtagcata catgacttcc aagattgcac gatctcttat ccctaacgtt tttgtcgtat
    19081 caggtgcttc aatcagcttt tctacttctg tcagagtcaa tgtacttggt aatttctgtg
    19141 ctttcttcgg cgtatcgata tgttgcatcg gatcatgatc agtgatacgc tcttgccgta
    19201 aaaactgatg gaatcttctg agacttgaga tcatccgagt aacagttgct gtcgcttttt
    19261 tttcttcatg caacttctcc agaaaacgga tgactaaaaa gcgatcgaca tcctgccatg
    19321 aagcaatttt ctgctcctgt agaaaatgca agtactgttc caaatcacgt tcatagcttt
    19381 gtcgtgtatt ggcggacaat ccccgttcaa tattcaagta gtggagataa tcgttgattt
    19441 gctctttcat aataatcccc ctcatgcctc tctatcatat cagataccgc ttcattctga
    19501 tagtcttttt ccataatctg agcaaagaaa aagccgtcta gacactcaga cggactggtt
    19561 tcttttttct ttttcatagc attcctgaca gattccatgg aacgttagcc gatgatcttt
    19621 tactaaaaaa tgataacgtt cttctactac ttgctccact tcaagtaaaa gatcctcttc
    19681 tacttcctcg atcgtgccgc agttcaagca tagtaaatga tgatggaaat gtttggcgcc
    19741 ttcttttctc aaatcataac gggctaatcc atcattaaaa cttactttat cgacaatttt
    19801 caattctgtc aagatttcaa gggttcgata aacagtagca agtccaattt ccggactctt
    19861 ttttttcact aaaaagaaga tttcttctgc agataaatgc tctttctcat tttccaaaag
    19921 cacaagcaga gtagcttctc tttgcggtgt cagcttaaac cccgcttcat gtagctgctg
    19981 ttttgttttt ttcatcgccg aaatagcatt catgaccatt tactcaaccc caacaattta
    20041 taataattac aaataaaaaa agatataaca ctatgttaat taaaattatt ataagctagt
    20101 taagaaaaac atgcaaggga ttctcaatta gattttttga ctagttttgt cacaccgttt
    20161 tcttgtgtaa tcaatccttg tttcaaaaga ttacctaatg cgcgcttaaa tgctcctttg
    20221 ctgataccaa aagctttcat gatttcttct ggatccgatt tatctgtaaa gttgatttgc
    20281 tgatctgcag aacgttctaa aatggttaaa atcatttgtg catcatctga tatagcttca
    20341 tatgctcttg gcttcaatga aagattcagt acaccgtctg gtcggacacc aatcactcga
    20401 ccttttattt gctcgcctag acgaggttca ttatatcgtt cagaaggatg gataaaccca
    20461 agatagaaat cttccgttaa aacgtacgta cctgttaatt tcaagcggta tgctgttcct
    20521 atcacatctt tgtttttcat ttcttctgtt ccatatttgc ttaaagattt gaatattttt
    20581 tcatctgcta aactgcccca aatacggtct tttgcatcaa ctctaaggga aatcatcaag
    20641 cgatcccctt ttttaggcca tagctctgtc attgtcggca attcatcaag agatacgaca
    20701 aagtctttat ctggaagacc gatatcgaca aacgcaccta aatcttttcg tgtagcagta
    20761 acgattccaa aagcgtaatg accgatacgg cttttaggaa tttgtgtagt gatccgattt
    20821 tcatgttttt gattttggta gccgaatcct tccactgctt caccgatttt gtgttcacct
    20881 tctgatttgc ttaagccaaa ggtttgtccg tttttttgga caaagtagtt tttttcattt
    20941 tcatcaataa taatacctgt aaatatagtt gctaataaat cgttcatttt tttctccctc
    21001 tatagtgaaa ggttttctta atacatttct ttgctatcat aacataatgt tatctacttg
    21061 aatagctgag ccttggtttg gaaaggtgaa aaaaggagta tgacaacagt tgtgtcatac
    21121 tccccttttt cgaataaacg gtgttaaacg gcaggctcac agcctctttc atttgcctaa
    21181 aattaattgc tcccatttcc tccgaaccat ccagttttac agttgatggt tacgatgtaa
    21241 aatggcaaag cactttctcc aatgcctttc atcaatgcag attttgttga agtggcatat
    21301 agattgtaat agccttcccc attttcgatt ctggcttttt ctaatctgta tcctccttca
    21361 acgatatatc cacgttggat acatgtagca atcactttgc tttggacacc tggtgcccac
    21421 gtccatgctg gattagatgc atcggcaata tcacttgctc tggcagattt gattggctga
    21481 ttcgctgagt ttgaagagga aggggtcgag gatgccgctt cttgctgtgc tgcttgtgcg
    21541 accgcagctt gttgagcggc ttgagctgca gctgctgctt gagcggcttg ttttgcttgt
    21601 tcttcagctg cttttttcgc tttttctgct tttttcgctt cttctgcttt tttcttttcc
    21661 tcttcttctt ttttctcttt atcagtgagc atcttatcta cttgttgcaa ttgttcattc
    21721 aactgagcag atagatctgt atttttcact ttttctactg cttcttttgc cgtcttgtat
    21781 tggtcgcgtg ttgctgaatc cactactttt ccgtctttaa agactatagc tgttttttct
    21841 ttagctgttt gcaattcagc atattgattt ttagcttctg tttgtgcttg ttgaatcaat
    21901 tttccaaaat ctgtgttatc cgtttcgatt gcttggatct ctttatctgc tttcaacttg
    21961 atgtcatctg ctagatgatc atcgacgatg gcaggtgcta tgaaccactc attcactttt
    22021 tggatatctc ggatcttaga ttggatatct tggtaagttg tttctaactc tgaatatcct
    22081 ttttcattct taagactgga taaatcattt tttagttttg ataaatcttg atgaatcata
    22141 tctgcacgaa taaattgatg gtgatcatct gtataaaagg cagcaagttt ctcatgaagt
    22201 tcttccattt gttgctgtct ttttgcttct gctgctactt cggcttctac tttttgatga
    22261 tggtcataat aagcccagcc tcccccacct agtagaagaa ctgctgctaa agcgagatac
    22321 ggcacttttt ttgatttttt cttttgtgtc ttatctaaat cttttttttc tgggtttgat
    22381 tcttgaggcg ttttttcagt atttggcacg attgttattg gttctgtttc tgcttcaatc
    22441 agctcaggct ggttctcttc tttttctatt ttagcttcaa gagcagtgct atcttcactt
    22501 gcagattgag tttcagcatc ggcttcatta tttattccat ccgactctgt tgccttcttg
    22561 tctgcttttt cgagcatttc ttttttatct gcttccggca acccattcga tttctcgtct
    22621 tctgattgtt tttcagacaa tgtttcagtt ttttccggga tatccgtttt tagatcattc
    22681 gtttttagat cattcgtttt taatgattct ttacctgcag cgtttactcc actaatgtct
    22741 ttttcagtgt ctttttcagc ttcatgttct ggctctactt tgttttcctg tgcaattttt
    22801 tcactgatca ttttgagcag ctctttcgcg tgctcttctt ttgtatcttc acgatgtgct
    22861 ttgatataag cggatagtat cgaattttct tcctcattat cgacgatttc tttcagttca
    22921 gctactcttc ttttttgttt tgattcaagc gtttcttctt gattctttcc tgattcttcc
    22981 gaagtctcat gaacagttgc tttcttctgt tcatttttct ctatattagt tacttgatcg
    23041 tgagcaggtg gttcttcgtc ttctggttct gctttttcga ataattccat tacagattct
    23101 agaggtaaat ctttcaattc cgaccattcg attggatcgt ttaattctgt atcaggaaag
    23161 actgcatctt cagttgattt atcctgcata gctgcatctg tatttgtttc ttcaattgat
    23221 tcttttattt gctgatcttc tgcagttatt tgatgaccgc agtctggaca gatagtattc
    23281 tgctccattt ttttcgatcc gcaatttgga cattttttca tcacttagcg atcggctcct
    23341 ttactaggtt tacgctacca tttaatctat catattctat acgtcacgcc aatagcttat
    23401 ttttaggatt tacttaaaaa taatgaatga atttccttcc tatagaaaaa aaagcttgag
    23461 actgcgtagt ctcaagcttt ttttctttta aaataaacca aacagtttta ctgatttctc
    23521 ttctggtcaa tagttcatgc ttattctaag aaatctttta attgttttga acgagaagga
    23581 tggcgcaatt ttcttaatgc tttagcctcg atctgacgaa tacgttcccg agtaacacca
    23641 aatactttcc ctacttcttc caatgttctc gtccggccat catctaaacc aaaacgtaag
    23701 cgaaggacat tttcctcccg gtcagtcaat gtatctagta catcttccaa ttgttctttt
    23761 agtaactcat aagctgcgtg ttctgctgga cttgttgctt cctgatcttc tatgaaatca
    23821 cctaagtgag agtcatcttc ttcaccgatt ggcgtttcaa gtgaaacagg ttcttgcgcg
    23881 attttcaaga tttctcggac tttttctgtt ggaagatcca tttccgcacc gatttcttct
    23941 ggcgtaggtt ctcttccaag gtcttgcagc aattgacgtt ggatacgtat caatttatta
    24001 attgtttcaa ccatatgaac tggaatacgg atcgtacgtg cttggtcagc gattgctcga
    24061 gtgatggctt gacgaatcca ccatgtagca taggttgaaa acttaaatcc tttgcggtag
    24121 tcaaattttt caactgcctt catcagtccc atgtttcctt cttggatcaa gtcaaggaac
    24181 tgcatgcctc gtcctacata acgtttagcg atacttacta ctaaacgaag gtttgcttct
    24241 gccagacgct gtttcgcttc ttggtcgcct tcttcgattt tcaatgctaa cgctacttct
    24301 tcttccgcag ttaaaaggga gacacggcca atttctttca gatacatacg aaccgggtca
    24361 ttgattttta caccggttgg cgcagataga tcttctgttt ttgcttgttc agcttttttc
    24421 tcagcactct tcaagctgtg aattgatgga tcaccatttt catccaccac actgatacct
    24481 gcgtcttcaa tcttttggat caatttgtcc atttcatctg cattcaaagt gaatggtgta
    24541 gcaagctgat tggataaatc atcatataca acttgtcgtt tgggtttgtt ttctttgatg
    24601 aaagctgcta ctgctgcttc atacgttttt ttatctgttc catttgccat aaaagaaggc
    24661 cccccttgag tagctatttg gcttgtttta actgtttcgt taaattgatg atttcaatcg
    24721 tcaattcaag ctcaagctgt ttgtttccag ttcgtctggc ttcttgctgc atttgttttt
    24781 ttaatgttat ttcatcagct aagccagatt tgctgatcac atctagcaag tcttggattt
    24841 ctcgtggcga actttcttct gccaccggta aatattcgat ttccaccacc aaccgcctta
    24901 aattttcatc ttgcaaaaag tctaaaaact ctgcaaggtt gaattcataa tgttgtgaga
    24961 catatgcatc gaataaaaaa taaacttctg catatgcatc atgaataaac tgaaattctc
    25021 gttcttttaa aatattacgg acagaaactt ctttaaatgc acgatataat agaatctgct
    25081 ctgccttttc tacttgcgtc aaaggtctct ttttccgtgc agtttcttgt ctcgtaacct
    25141 gagtgtgttg actttcctga ggttcttgac gattttgctg atttgtcctt cgaagatcgc
    25201 gcagttgttt ttgtatcgtc tctctgccga tctgaaactc tgttgacagt tgagatagat
    25261 acatatcttg ttcgattggt gaaacaactc ggctaagttc aactagtaat tcttcgaggt
    25321 attcgatctt gtctttttca ttatctaaat ttttcccttg ttttaaataa cgtgatttaa
    25381 aagtaaagac tgtttcgcgc ccatgagcca ctaattcttg gaaagatgct tctccatact
    25441 tacggagata ttcatcagga tcaagctttt ctggaacgac tacgacagaa agtgctaagc
    25501 gactgttttg ccgaagcaat tcaatccctc gattggttgc ttcaaaacct gcgttgtctc
    25561 cgtcatagca aaagaccaat tctttcgtca gcttttccat ttgttggatc tgttgagctg
    25621 ttagacttgt ccccatagaa gctaatccag ttttgatacc agcctgccat gcagcaatca
    25681 catccataaa cccttcgaac aacaaaattt ctccgttttt ccggatatcg cttcttgctt
    25741 tatcaagatt aaataaaact tgtcttttat tgaacagttc tgtttctgga ctattcaagt
    25801 atttgggctg atctttttcc tgcccttttt caggtgcaag ccagcgtcct gaaaaaccga
    25861 tcgtctggcc ttgagaatta cgaagtggaa acatcactct ttgataaaaa cgatcagaca
    25921 tacggccatc atcatgcgtg acaaacaatc cagagcgttc taacaattca tttgaaattg
    25981 attcattctt gaatacttgc tgaagaaaag aacgctcatt aggtgcaaaa cctatgttga
    26041 attcttcaat taattctaaa gacaacccac gactttgcaa atattctaaa gctggctgtc
    26101 cagcttttgt atgcaatagc atgtgatgat agatttccgc tgctttttca tgagcagcaa
    26161 tcagtttccc cgtagcagat tcaggatttt ggacagctat agtacgctga cgccattgat
    26221 cttctaacgg tatctgttcg aagtctgcca cttttaccac tgcttctgga aaacttagac
    26281 cttctagttc ttgaagaaaa gtaaacacgt ttccgccctt accacaacca aagcaatgaa
    26341 aaatctgctt gtcttctgca acagaaaaag acggtgtacg ttcttcatga aaaggacaca
    26401 gccccaagta atttttacca gactttttca actggacata ttgtccaatc acctcaacga
    26461 tattcgtttg actgcgtact gtttcgataa cctcttgagg aatccgttga gccatttttc
    26521 caccccctgc tctattgata tcttttgggt ttaacagaag atatttcagg agaataaccg
    26581 gtaaaaaatc gcactaactg aaatcagtgc gattttaaag gacacaatta tacatgtttg
    26641 attgtatcat atttcattgc tttttcaagt ataacacatt tctatctcat taagcatcaa
    26701 tttgctactc ttttcagtaa ctgattttga atatcagcct tccagtacaa tactcgaaga
    26761 acaatattta cgagcatgaa tacaaagaag tatcctacgc tctctcccaa ccctaagttt
    26821 acttccaaaa ttccatggaa cagcattgca gctacatata tccctacgat cgtcgttact
    26881 gctaggaaga tgcgcaatgg gttgaaggga taacaagctt ttacaactgc catgcaactg
    26941 atcccaatca gcaagtaata catcaaagta gtagtttcag catttcccca tgcgaatgtt
    27001 tttccaagta gatacaccgc aatgatattc actactacaa gcaaggcatt tggtaaagca
    27061 ttaataagtg ctgtaggcaa ataacggtaa gtgatttttc ttttatcttg ttcaaaagat
    27121 aaaaagaagg aaggataccc ttcaatcgct aaatcaatca gcgtgatttg gatagggata
    27181 aacggaaatc caattgctgt aacggcacat ataatcgata aaataaatga ataaatcgtt
    27241 ttgataaaaa agatgcctga gactttcgtc acattgttga ccactcggcg tccttcaaac
    27301 aggacttctg gcaatgtagt aaaatcagag tccaacaata ccagattcgc aatctgacgt
    27361 gttgctccat caccttctgc catagcaatg ctacaatctg cttctcttaa cgccaaaaca
    27421 tcattcacac cgtcgcctgt cattgcaacc gttctgccgc tttctttcaa ttcatttacc
    27481 agcaatttct tttgctgagg actcactcgt ccaaaaaccg tatagcgatg agcagcttct
    27541 cgcacttcca tttcttcagt aatttgagaa agatcgacat acgcatcata attcggtaat
    27601 cctgcacgtc tagcgatatt ggagacagta aaaggattgt ctccagaaat cactttcaga
    27661 tccacacctt cctcatgaag ataagctagt gtttcttgag cgttttttcg gattggatca
    27721 tcgatttcaa acagagcaac cggctcaaca aatgctggtt tttcttcgtt caattgctga
    27781 tttggagcga tccctaacat aagtacgcga tagccattcg cttgggctgt ttttagactg
    27841 tccggaagcc gatcttcttc caccaaacgt tcaggcgctc ctacgtacac gactcccagt
    27901 tctggaaatt ccatcgcgcc ccatttgcga tcagaagaaa atgcgacttt gtttgtcaca
    27961 tcataccgat tagaaagctc aaaataatct ctcatagccc gcatagtgat gttgttatca
    28021 ctgctttctg ccagatacgt ccccataatt tctgggatgg ctttctcgta cagcggatgt
    28081 agtgcttcta cccgctggat actcattttt ccttcagtaa tggtccctgt tttatccaag
    28141 cataatgtat cgacatgggc cagcgtttca attgaataca tgtcttgaac aaggattcgt
    28201 ttttttgcca gtttggttac ccctgtcgtt aatgcaatac ttattagcaa taccaaaccc
    28261 tttggaagca tgcctaacaa agctgctgca gatgcaataa cagaaatttt gatagaagca
    28321 tcgcggatga acagtgcttc caaaaatagt atgatcccca aaggaataat cacgaaacta
    28381 gtgaatttgg atactttgcg aatcgaatct acaagttcag aacgaatagc tttgtgagtc
    28441 tttgcctcgc tagtcaagcg gacagcataa ttatctgcac ctactcggat aacttcagcc
    28501 atgacagaac cgcttgtcaa aaagcttcca gaaagcaatt cagctccagt ttctttgaca
    28561 atcaaatcag attcgcctgt caaaagagct tcatttgctt ctgcccgtcc agatataacc
    28621 tgtaaatcgg ctggtatctg ttctcctgct ccgattttta ccaaatctcc taataccagc
    28681 tctttagtag aaatcgattg ttctttaccg tcccggatca ctagtacttc tgattgggaa
    28741 acaatcgata atttccgtac aagatttctt gcatgtattt cttgataaat tccaataacg
    28801 atattgacga caataattgc taaatacgcc atgttggaat acgcacctac aaataacaga
    28861 caaatcccaa taccaaagtt cagaaaattg aataacgtca atatattatc gctgaatatt
    28921 tgttttgtac tttttgatgc atcttcctgg tagtcatttt gctggccaga agccattttc
    28981 tgaactacct cttgctgtgt aagtccattc attttctttt tctccatttt ccgccaactt
    29041 tcaaaaataa tcacgtgttt aagtctagcg tgttccaaga ttaaaaacaa cttgttttta
    29101 taaaatttat agaaaaacac acgaatgatt ttacaaaaaa gatgccaacc tttttgtttg
    29161 aatcacccgt gtgctgtttt tattatcaat ctatctttac ttatattgtt cctccaaacg
    29221 gctcatccac caacctagtg ctgtaccggc tataaaaaga acaggacaag ctagtacccc
    29281 agcaactccc aatcccgcgt aatcggtagg aatgatcata atcgttgaag caaaaactac
    29341 acctaaaatg aaatggaata gcttgctata tgcctttgag aaaatgtaat ccatgatttt
    29401 tgacaatcca agaacacaaa gaatcccacc aattccgatc ggcacaatca cttcaagtcg
    29461 tacttcttta aatccgtcag ccatcgcttt atacaatccc atatacacca aaaaattaga
    29521 agggctcaat cctgggacga tcatacccaa accaatcaat ccgccggcaa tcagccatgt
    29581 tccaaaattc tgatcgactt gcccgtcaaa taattgagcg ccgtataata aaaacaaata
    29641 agccagaaca aaagtaacaa ccatgatgat cacatcagaa gtatctcttc cttttttccc
    29701 tgcttctttc cataacgcag gaacagttcc agcgatacat ccgacaaaaa accataatat
    29761 cgttgtttca tacgcaccta ataaaaaact gactaaaaaa gaaagtagaa agatgcctgt
    29821 cactccgcct agaccaacag gcagaaaaaa caaaacattt tctttgaaat tttttgtaag
    29881 gtgggctaaa aaagaaatca gccgctcata catgccaaag acagctgcta atgcaccacc
    29941 gctgactcct ggcaagataa atcctgaacc gataaacatt cccttcacaa accgtaacat
    30001 ccagtctttg gttgtgtacc ccactttttg attttccatt ttttgccttt tttccactat
    30061 ttgcgcactc cttctttcat ttaattttat tcatgattga acatttcctg ctattttact
    30121 taaaaacttt ttcataggtc tctattattt tttcattcat tttgacaata tcaggataaa
    30181 acatgttacc tccattatga tagagatagc cgccattgta aagcaaggac tggatccgcc
    30241 aatatcggta ttgtttccct tcctcatttc ctaaaagtgg gctcagcaca tctttagaat
    30301 acgcttctgc taattctact gtgttttctc cgccatgatt ttttacatag gcaatataat
    30361 ctttcccaaa attatatgct tggacagctg tccataaatc gcatccttgt ttctttgctt
    30421 ctactatcat ttccgccaaa tatttgacac cttgatcaat actgtcagct ggattatcaa
    30481 tgatctcagc ttctccatgc agactttccg agctccgcat gagatctgtc ccttttcctt
    30541 ttgattcggt caatattatc gccttgatca aattctcgta gttttccata tgataatttt
    30601 ttgcagctgt tgttatttct atttgatagc tttccactcg ttttaccgaa cgataagttc
    30661 ttcctcctaa aaacaacacc gcaccaataa ccgtcagaat gatcatgtaa aaaagaatac
    30721 ggataataga taatcgtttt cgcttcattt catcacctag ttattttgac atcttttttt
    30781 atcttactat ttctttatct tcttggctat ttcttccttg atttctgtgt acgccttaag
    30841 aaaaaatgaa ttttcctgta ttttttgtca tcttacgaaa atgatcgttg tttttcagta
    30901 aaaacaattg cccaatgact gatataatcg ttatacttta cacagtaggt gattatgtga
    30961 aaaaagaaaa gaaatatgca aaaatgccag atgggagcga aatttactat gaaaagagcg
    31021 gtcaaggctt ccctctcttt ttactgcatg gcaatgacgg cagcggccgc tttttttcag
    31081 aacaggtccc tgtactcgaa cgctactata ccgtgtatct agtagatagc cgaggacatg
    31141 gtcgttcgac aaacgaagca tctatgttga attttcagtt aatggccgag gatctgaata
    31201 cgatcatgtt gctggaaaaa atcgatcagg cagattttct tggatttagt gatggagcaa
    31261 atctagcact ggtatttgcc agcagcttcc ctaaaaaagt tcatcgattg attttaaatt
    31321 ctggaaacac gcttgttaaa ggcgttcgtt tttctgccag agtcatcagt aatattcatt
    31381 atgcatgggt atggttactc tctttatttc gcccaagtct tcgcaaaaat cttttagtta
    31441 tcaaactttt gctgcatgac atcgggctta cagaaaatga tttaaaaaaa atcaactccc
    31501 ccactttgat catcgtggga aaaaaagatg taatcaagct aaaacattct ttgtatatag
    31561 ctaaaactat accaaaagct tcctttgtgc ttgtgaaaga acaaggacat gaattagcta
    31621 gaaaagatcc tgaacgcttc aatcgagaag ttcttcaatt tctttctgaa acataactat
    31681 taggaataga aggtgattta tttgttaaaa aataccatac aatggctgaa aaaccaccta
    31741 ggactgttca aaacaatctt tttgatttct gtcattgtca ttattgtcgg tgaattgatg
    31801 tccatcggta aaactctatc tatccagcaa ttaggagaaa cgtttgccac tatacctgta
    31861 tggaaaagcg gacttatgct gttgattggt cttgtctctg tgttacctat gattggttat
    31921 gatattattt tgaatcggat gcttgaacaa aagcagaatc ctcgttactt gtttgagaca
    31981 agctggctga tcaatacaat caacaatatt gccggtttcg gcgggtttgt tagtgtgggg
    32041 cttcgctctg aactatatgg acaaaagaaa gagagcagaa acgtgatcca agcattatcg
    32101 aaagttttct tgtttttgat ggctggactt tctatttaca gtctgctttc ttttttactt
    32161 gtagtcttca cgccagttgc tccttttttg aagcaatatt ggatatggtt gatcggcggc
    32221 ggtctatatt ttcctgtcgt ctttttattt actactttca aaaagaaagg attccttggt
    32281 ggtacctcac ttaaaactca aggacagcta ttgctagttt cattgttgga atggaccggt
    32341 gtcctgttta gtttcctttc tgttggttat ttaatggaaa tccggattga tctgtggcaa
    32401 acaattccgt tattcattgc tgcttcagtg ataggaatcg tgtctatgat tccaggagaa
    32461 attgggagtt tcgatgtaat gatgattatc gggttgtccg ctattggtgt gccaagagaa
    32521 atagtcgttg tgtggatctt gctctatcgc ctgttctact acatcattcc gtttttgatt
    32581 ggaatcgtct ttttcttcaa aaatatcggt tccacttttg atcagcgtta ttctggtatc
    32641 cctaaacaat tagcaacaga aattgcgcat aaaatcgtgg ttgtcttatt atacttttct
    32701 ggaatcatgc tagtactttc tgccactatc ccgcaagctt ttacagagtt tcgctggctt
    32761 catagtttga acccattgaa atttcatttt attatccaat ttcctagtat actattggga
    32821 tttcttttaa tcattatggg cagagggatc gcagcacgtg tgaaacgagc atatttgcct
    32881 acaatcttct tgattgttct agctttactt tatgttttat taagtgattt tagtttcaca
    32941 ccggtggttt tcttgtctat tctgcttctg atcattcttg cttctaaaaa cgaattattt
    33001 cgggaacaat tgatctattc atgggagtgg cgtacgatcg atggaatcat tattggtgca
    33061 ctgtccttgc tttacatcgt tattggggta tacaatctgc cggatttccc tcatcggcgt
    33121 caccatttta tttccttttt cttatttcct tctgaaaaga tttggttttc gggattgttg
    33181 gccatcatcg ctgttagttt catgatcgtt ctatttgttc atttccttca aggagaaaag
    33241 aaacagattg gcgaagcctt taatgaagaa aaggccctga agatccttac aacttatgga
    33301 ggaaactccg atagtcaatt gatcttctta aaagataagc ggatgtttgc ctatgaaaaa
    33361 gacggtgaac caactgttct tctccaattc gcttgcttca ataataaatg tattgtaatg
    33421 ggtgatcctt ctggtaagaa agaagatttt ccagaagcaa tcgaagcttt tatcgaagaa
    33481 acagatcgac tatgctattt accagttttc tacgaaacaa gcgaagagat tgttatgatt
    33541 ttgcatgaat tcggctacga tttcattaag atgggggaag aagcttatgt agacctgaat
    33601 agttttacaa cttcagggaa aaaaatgaag ggcactcgtg ctgtcttgaa tcggatcgaa
    33661 cgagaaggat ttacatttga tgtcttacaa ccaccatttt ctactgagca aatgagtact
    33721 ttcaaaaata tctctgataa ttggttgggt tcacgaaagg aaaaagggtt ttctttaggc
    33781 tttttctctg aagattatct acaaagagcg cctattgcag ttgtcaaaaa tacccaagaa
    33841 gaaattgtcg cttttgcaac gatcatgcct acttacacga acaataaagt aggaacgatt
    33901 gatctgatgc gatatgatcc taaaaaagca ccttctggca gtatggattt cttgttcttg
    33961 catttgtttg cctatatgaa agaagaacat atccaatggt tcaatttagg gatggcccct
    34021 ctttcaaatg tcggaacttc cagaaagagt tttttacagg aaaggattgc atacttggtt
    34081 tacgagtttg gttctcattt ttattctttc catggattaa gagaatataa aagcaagtac
    34141 gctacaaact gggtctcacg ttatactcta tattctagag atagttggat tgcatatgtt
    34201 atgattgctt tgctgattat tgataatgcg ccagttgagc agacacaagg tagaataagt
    34261 ggtttgaaaa aatggatacg aagaaagtat taaaaactgc ataccgatag atgaacactc
    34321 tcatgatgaa taaaagagac tgaagtaaaa cgaaagttca tgcttctaca atctcttctt
    34381 atacaattga agaacaaaga gcggtccaaa cagaaatcct gtttggaccg ctcttttatt
    34441 ttaaacattt ttcattacca aaatattcga gtcgcgtttc ttatgcttcg atctctgtaa
    34501 cgatacctga acccactgtc cgtccgcctt cacgaattga gaatgtcgtt ccgttttcaa
    34561 cagcgattgg atggatcaag tcaacatcaa tcgtcacgtt gtcaccaggc atgaccattt
    34621 ctgtatcttc tggcaatgtg attgttcctg taacatcagt tgtgcggaag tagaattgtg
    34681 gacgatagtt gttgaagaat ggagtatgac gtccaccttc ttcttttgtc aacacgtaca
    34741 cttctgcttt gaattttgta tgtggagtaa tggaacctgg tttagcaagc acttgtccac
    34801 gttcgatatc gtcacgttgg atcccccgta acaacacgcc tacgttatcc ccagcttccc
    34861 cgtaatctaa cgttttacgg aacatttcta cacctgttac aactgctttt tgtgtttcag
    34921 gtttgatccc tacgatctct acctcatcac cgacacgaac agcaccacga tcaatacgac
    34981 ctgaggcaac agttcctcga cctgtaattg agaagacgtc ttccactggc aatagtaatg
    35041 gtttatctgt atcacgttct ggtgttggga tatattcatc tactgtatcc atcaattcca
    35101 taatagcagc ttcagcatct ggatcgcctt gcaatgcttt taatgcggaa cctttgatca
    35161 caggagtgtc atcgcctggg aaaccatatt cgctcaataa ctcgcgaact tccatttcta
    35221 ccaaatcgat caattcttca tcatcgacta aatcaacttt gttcaagaaa acaatcaggt
    35281 attttacacc aacttggcgt gacaataaaa tgtgttcacg tgtttgaggc atcggaccgt
    35341 cagttgcaga aacaactaag attgcaccat ccatttgtgc tgcccctgta atcatgtttt
    35401 tcacatagtc cgcgtgtcct ggtgcatcaa tatgcgcgta gtggcgtttt tctgtttcat
    35461 attctacgtg tgcggtattg atcgtaatcc cacgttcacg ttcttctgga gctgcatcaa
    35521 tactagcata gtcttgagga tttgctaggc cttttttgcc taatactgta gtgatcgcag
    35581 cagttaaagt agttttccca tggtcaacgt gtccaatcgt accaatgtta acgtgtggtt
    35641 tacttctgtc ataatgttct tttgccataa tttacaaacc tcctaaataa tatagatcac
    35701 tgaaaaattt cagctctcac ctttttatta tagtcttttt ttgactaaat caaaaagaat
    35761 acgtccgact tactttttgt aagattcgaa ctaagcttat tatacgtcaa tattggtcaa
    35821 agtcaactaa gagcactcgc ttgtcttata cgcgaaaaaa gtacctttta taaataaaaa
    35881 aatgtgtttt ttgagaaaaa cttcaagata gtccttaact ttcttttgaa gcaataagaa
    35941 caaaatgagg aaaagtttaa aattcagctg ttgccaacaa ctttcaatag acgaaatctt
    36001 ctatttcgtt aataataagg aaagaggtga agtacatgaa aaaattaatt ggcgtacttg
    36061 tcgttttagg catatttctc ggcggaagtt ttgcagctta tcattatttt tacggcggcg
    36121 aagcttatta tacaaaaatc acgacgaatg gcgaaatgtc agctacccaa gcagataacg
    36181 gagagaaatt cacgacttat acctacaagc aagcggctta taataaagat ggtgaaaaaa
    36241 aacaggtgac gcttcgcgaa gaaagagaac atccattaaa gatgaatgcg tatcttaaac
    36301 tcaaagtcaa tcctcgtaaa ggtgtattaa gctgggaaga aataaaggct tctgaagtgc
    36361 ctaaaaatgc ggcagaaaaa atcgataatt agtaaatggt cttaaaattt tattagataa
    36421 aa
//
LOCUS       DAMEET010000019        35983 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.019, whole genome shotgun sequence.
ACCESSION   DAMEET010000019 DAMEET010000000
VERSION     DAMEET010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 35983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.019"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(5..1282)
                     /locus_tag="PB780_000808"
     CDS             complement(5..1282)
                     /locus_tag="PB780_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732879.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(1361..2005)
                     /gene="pcp"
                     /locus_tag="PB780_000809"
     CDS             complement(1361..2005)
                     /gene="pcp"
                     /locus_tag="PB780_000809"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDE0732880.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(2204..3148)
                     /locus_tag="PB780_000810"
     CDS             complement(2204..3148)
                     /locus_tag="PB780_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HDE0732881.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(3150..3836)
                     /locus_tag="PB780_000811"
     CDS             complement(3150..3836)
                     /locus_tag="PB780_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HDE0732882.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            4310..4630
                     /locus_tag="PB780_000812"
     CDS             4310..4630
                     /locus_tag="PB780_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HDE0732883.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            4784..5689
                     /locus_tag="PB780_000813"
     CDS             4784..5689
                     /locus_tag="PB780_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDE0732884.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            5686..6459
                     /locus_tag="PB780_000814"
     CDS             5686..6459
                     /locus_tag="PB780_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDE0732885.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(6640..8064)
                     /locus_tag="PB780_000815"
     CDS             complement(6640..8064)
                     /locus_tag="PB780_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDE0732886.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            8275..8721
                     /gene="nrdI"
                     /locus_tag="PB780_000816"
     CDS             8275..8721
                     /gene="nrdI"
                     /locus_tag="PB780_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDE0732887.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(8786..10768)
                     /locus_tag="PB780_000817"
     CDS             complement(8786..10768)
                     /locus_tag="PB780_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDE0732888.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(10782..11678)
                     /gene="murQ"
                     /locus_tag="PB780_000818"
     CDS             complement(10782..11678)
                     /gene="murQ"
                     /locus_tag="PB780_000818"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDE0732889.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(11681..12742)
                     /locus_tag="PB780_000819"
     CDS             complement(11681..12742)
                     /locus_tag="PB780_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDE0732890.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(12953..13519)
                     /locus_tag="PB780_000820"
     CDS             complement(12953..13519)
                     /locus_tag="PB780_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDE0732891.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(13547..15049)
                     /gene="eat(A)"
                     /locus_tag="PB780_000821"
     CDS             complement(13547..15049)
                     /gene="eat(A)"
                     /locus_tag="PB780_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HDE0732892.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(15307..16428)
                     /locus_tag="PB780_000822"
     CDS             complement(15307..16428)
                     /locus_tag="PB780_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDE0732893.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            16554..17495
                     /locus_tag="PB780_000823"
     CDS             16554..17495
                     /locus_tag="PB780_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDE0732894.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(17645..18541)
                     /locus_tag="PB780_000824"
     CDS             complement(17645..18541)
                     /locus_tag="PB780_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HDE0732895.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            18810..20150
                     /locus_tag="PB780_000825"
     CDS             18810..20150
                     /locus_tag="PB780_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDE0732896.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            20368..20811
                     /locus_tag="PB780_000826"
     CDS             20368..20811
                     /locus_tag="PB780_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HDE0732897.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(21315..22733)
                     /gene="nhaC"
                     /locus_tag="PB780_000827"
     CDS             complement(21315..22733)
                     /gene="nhaC"
                     /locus_tag="PB780_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDE0732898.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(23136..24359)
                     /locus_tag="PB780_000828"
     CDS             complement(23136..24359)
                     /locus_tag="PB780_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDE0732899.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFLYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(24390..25229)
                     /locus_tag="PB780_000829"
     CDS             complement(24390..25229)
                     /locus_tag="PB780_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDE0732900.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(25246..25926)
                     /locus_tag="PB780_000830"
     CDS             complement(25246..25926)
                     /locus_tag="PB780_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDE0732901.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(25928..27001)
                     /locus_tag="PB780_000831"
     CDS             complement(25928..27001)
                     /locus_tag="PB780_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDE0732902.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(27057..27295)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(27338..27571)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(27648..28319)
                     /locus_tag="PB780_000832"
     CDS             complement(27648..28319)
                     /locus_tag="PB780_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDE0732903.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(28406..31084)
                     /gene="mgtA"
                     /locus_tag="PB780_000833"
     CDS             complement(28406..31084)
                     /gene="mgtA"
                     /locus_tag="PB780_000833"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDE0732904.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            31743..32180
                     /gene="ndk"
                     /locus_tag="PB780_000834"
     CDS             31743..32180
                     /gene="ndk"
                     /locus_tag="PB780_000834"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDE0732905.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(32202..33068)
                     /locus_tag="PB780_000835"
     CDS             complement(32202..33068)
                     /locus_tag="PB780_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDE0732906.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(33228..35321)
                     /locus_tag="PB780_000836"
     CDS             complement(33228..35321)
                     /locus_tag="PB780_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDE0732907.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT        10417 a         7556 c         6354 g        11656 t
ORIGIN      
        1 ttttttatag taattccttc agatactttc ttgcctcatc attgaaacgc agtgattctt
       61 ctaacttctg ataatattcc aacgcttgtt tctgagcttc agtaaaacga taatgttttc
      121 ctttccattt gtcgattgtc tcttgcacat ttccagattg gatatctttc actactagtt
      181 gccatttttc ttttgagact gtacggtctt tcttatcagc agattgtata gcttctcgtt
      241 ctttacgcaa tacttttaaa aaaccatttc tgactatccg aggaatgctg tcgaagaacc
      301 cttctgtaaa accatagcga caatattgct tccaaataaa tagatagatt tttggttgga
      361 tgaatcgttg gtttttcgca aaatattcca tctgagcagt gacttctggt gttcgttgac
      421 gttttgtctt tctcaacacg gaaataaatg tttcaaaata agcattagga tgtactggca
      481 cttcttcata tacgatggat tgccaatctt tacgtaaaaa tgcgcgatag ttgatcaaga
      541 attcttctac atcctttact aattcttctg caaagtcgtc aaatagttct ttttttatct
      601 gtcgcttttt tgactgccca ttgatgtgtt cacttatttt cttgatttca ttttccaatg
      661 cttgataatc cgctatgtaa atcggcattt ctacttggcg aaattctgcg agccgctgag
      721 ctaattttat atccggtaaa tatggctgtt tcatccgacg attttggtac aagacaaaac
      781 agtatttttg ccatagtcct tcaggaaaat cgatattctt ctgcttaagc cataataatt
      841 cgtctaaggc cagatcaact tttgtcattc cctgatggat cgaagctgcc actgtcggag
      901 agacatgcag gtgatcggca aagtggttga tgcctagttt taggatcttt ccggtttttg
      961 gcgatttaac gatgtattgc acttttaatt ctctaccaca ctcacaatac aatttttgct
     1021 ggcttcgttt tgttcgaaaa aacggattga tcttgaagtc agaaaacacc caatctgttg
     1081 cagctaaata atgttcttcc ataaaacgag agctgattag atatcggcgg tgcttatcga
     1141 ctagttcccg ctgttttttc gttaattttg catagatctc ccaacgttga ctgcttgtgt
     1201 attgtttaga aattttgatg ggatagcttt tgctgatttc ttttggaaac cattcttcaa
     1261 agacggattg ccattcttcc acggctgttt ctttcctttc taattacaaa aagctggcta
     1321 ctaacataac gttagcaacc agccagtgtt tcttgctaaa ttagtgagtt gttccggcat
     1381 ttccggaaat atcttcttta ttttctactg ctgcttcaac tgcataagtc aaagaagaag
     1441 caatgtcttc taaagacata gaaggcgtac ccactggacg atcgatcact tgatcgggtg
     1501 agaatggcac gtggatgaac ccacctcgaa ttgccgggaa ttgtgtttcg atcaaatgaa
     1561 gcaaacgata catgatatca ttgcagacaa atgttcctgc tgtataagag atgtacgctg
     1621 gtagaccatg atcttgtaca ttttttgtca ttgctttgat tggcaatgag gtaaagtaag
     1681 cggctggacc gttttcttct aaagccgtat cgaatggctg atttccttca ttatcaggga
     1741 ttcttgcttc tgctagattg attgcgacac gttcgaacga gactgctgac cgcccacctg
     1801 cttgtccgac acagatcacg acatctggtt ggtgctgttg gatggcttct ttgactactt
     1861 gactgctttt ttcgaatacg gtcggtactt ccactttgat gatttcagca ccgctgattt
     1921 catctggcat tttttttact gcttcatagg ctggattcac tttgtcccca ccaaatggat
     1981 caaaacctgt tactagtact ttcatctgtt gttcctccgt ccgattgatt taacatatgg
     2041 ttatctttta tcataccatt ttccttgaac agcgtaaata aaggaaacaa tagtttaaat
     2101 aacataacag atcaaactta atatattctt ataaaaaaat agtgttccac tacaatgtta
     2161 gaagaggttg cgacaattat tctgtcacaa cccctctctt ctattattta aatagaatca
     2221 tataagcaat ttggaaaact agcataaata acgcaataaa caactggttt ttgatcactc
     2281 cgtatttgtc tttcatctcc agcatcgcta caggcacgat attgaagtta gcagccatcg
     2341 gtgtcagcag ggtcccacaa aaaccgcaag tcaatgcaac cattcctatt aacgctggat
     2401 tcgctccatg actaaatacg aatggtgcac cgatcccaac cgtcatcacg gtgatggccg
     2461 caaatgcatt ccccatgatt accgtaaata tcaccatgcc aagagcgtaa atgatgatgc
     2521 cgatgttcac atttccttct ggaacgatcg tacctactgc attggaaatc actgtcccaa
     2581 ctccagcgct tgtaaatacc gcacctagag aagctaaaag cattggcaac atgcttaacg
     2641 gccctactgt gcccagcata tctgctgcat catctaaaaa tgtacttggt cgattatctc
     2701 gagaaaagaa catcaagatt agcatggctg caaaaacacc gacgcccacg ccgactaaag
     2761 cacctaaatt ggtaaataga gcaaacaaga tcgcaaagac accgatactc aaagctggaa
     2821 taaaaatttt cattcctagt ttgtctgaca ttttttccat atattctttt gatggaagcc
     2881 ggctttctcc tttgctgact ttcttagcaa ttgctggtat ggtcatcgca aagatcaagc
     2941 caccgttcac gattgccgga agccatcttc ctcctgcaat aactacccct aagaccgtcc
     3001 agaaaaaggc tgacccataa cgatgaggat tggttttatc tgttaaattt cttactccag
     3061 tgtaaatcaa tacgagtccc atcacgatat aaaggatctc cagtaattta tcgcctaata
     3121 aaatatcagg atttgtaaaa aagtccatct tattgcccct ttcctggata atatttcttc
     3181 gctaatttct tttctttaag atagaagtag acacttgcca caactaaagc cgtgatagcc
     3241 actggaactt ccactaaagc aagctgtccc aatgttacgt catatcctag atctttcagt
     3301 gttgattgga ctagcaacgc ccctgctcct ccgatgaata gtacttggcc aaagaaccag
     3361 cagacatttt ccatagctga agccatacct tttagttctt cttcatgctt aggatggact
     3421 tctaaccctt ttgattcgat ggtccccaat gccatcggta aaatgattgg acgaacaaat
     3481 ccagcaactc caccaaaact cacgttaaaa gccgcaaaga tcattcgaaa gatcccataa
     3541 atatctacaa tcataccagc agatactttc ttgaatcgct tgatcaagga agccgctgat
     3601 tctttcagac catttctttc aagcgttcct gttgccaaca taatgatgat aaaaatcgcc
     3661 atcccgcgat tgtttacaaa acttgtacct aaaagttcta acattccttg aatgccaaga
     3721 ccgccagtca aagctgtaac aactaaagca accattacga tcaatatcga gtctaattta
     3781 aatgcaaacc cgagaataac aatcaagacg cctaaaaggc taagataatt ttccatatat
     3841 tttcctccat aaaatgacaa tattttttta attataaagc aaaacacata aaaaatatag
     3901 ttgttttctc acgaaataga cactattttt aaatattttt cgttattttt ttatcacaaa
     3961 aattatctta cttattgtaa ggtaaagctt tttttaattt attgcccgat aatatataga
     4021 aataaggtct agttactgtt ttttttaaat gattaataaa taacaacttc tttctatgaa
     4081 attgcaaagc attcatcttc ttttccgcta acgatatatc cgtcagtgct tggtacactt
     4141 tactaaaaag tatgagattg ccgatatctg gataggtgtg ctatactaca gaaaaagata
     4201 tacaatctgc caccgggtag gataaagagg atgatcattc cattaggtct ttgaagggat
     4261 gattttcctc tttttgtgta tttttttata gttaaggagg aatgtaagta tggcatggat
     4321 ggaattaatc atagcaggaa ttttggaagt attctggtca acgatgatga aatggagtga
     4381 tggtttttct aaaatcaatt attcttttta cacagtgatt ggcatgattg ctagttttta
     4441 ctttttgtca aaggcaatca aaagtttgcc gatgagtctc tcttatccta tctggacagg
     4501 gattggcgca gttggttcgg tcataattgg tgtggtcttg tttcatgata agctgaatct
     4561 ttccacttgg ttctttgtgg ggatgctact catcagtatc attggcatca agattactag
     4621 cggccactag acacttggaa taagtactga tcagaaaaaa taagcctttt tatttaatat
     4681 tcaatttttt tatgaaaaaa taagaaataa gtccaatgac ctagcgactt ctccactcat
     4741 caggtgtatg cttgtactat ctatagaaaa ggagtgaata tttttgaaaa gtctttcggt
     4801 ccaagaatta tccaagaact ttggtcacaa acaagccctc agcgatgtca gttttagctt
     4861 atcccctggc aaaatcgttg gattagtagg tcctaatggc gctggaaaaa caacgatcat
     4921 gaaagctatt ttaggactga tccattactc agatggaaca atctgtattg gtgaaaatga
     4981 gatttctccc tctagtcata aagggttgga acaagtagga gcattgatcg aatacccggg
     5041 tatctatcca ttcttaacag gctatgatca tctgaagtta ttctctgaaa ccaacgatgt
     5101 atctgctatc gacactatcg tgaatcagct caagatggaa aagtacattc acaaaaaagc
     5161 gaaatcctac tcgttgggaa tgaaacaaaa attgggaatt gctcttgcct tactgaacca
     5221 tccgtctatc gttattttag atgagccgat gaacggtttg gatccacaag caacaagaga
     5281 tgtgcgaaaa atcattaccg acttggctac tcagggcacc tctttcttga tctccagtca
     5341 cttactcagc gagcttgaaa aaatggtcga tgagctgatc ctgatcaatc aaggaaagat
     5401 catcaaacaa tgtacaatgg ctgaattgaa ccaaacagcg caagatttca tcgttatctc
     5461 tacaacaaat gatctagctg ctcgaacgat cttgacagaa gctggatata ctttggaaga
     5521 tcctgataaa atcaagcttt taaaagaaaa cgaagaatta ttaggaaccg tgatccagct
     5581 attgtctgca caaaatatcc atgtgacaga cgtccagcat ctgacgaatg atttggaatc
     5641 ttctctattg gaaattctgg attcagtaaa gggggaagaa gaaaaatgac attgcttgta
     5701 aaacaagaat tatttaaatt gatcaagaaa aagtcaactg ctgttttatc tgttctatta
     5761 gttgttttgc tcataggaac agcacttcta gccaaaaaat acacgacgat cattgatcca
     5821 gttgaaatga ctgctcagct ttttagtgcc acatcttgga ttgtctttat catgattgca
     5881 gctgcaagca cgatcatttc gatggaagca caatatggta cattgaaaaa tctcctttat
     5941 cgaaagtatt ctcgaggaga aatattagta agcaaatgga tcacattagt tatttattct
     6001 gtttatttat accttttagc tattattgtc actgtgttga tgaaactgat tcttttccct
     6061 tcgatttcct tcacggagaa ggtttctact ggtcaaacac tgatccaaag cttgttcact
     6121 tatactttgg gtagttatat cggtttatgg ctgattttaa gtttagtgtt gatgattgct
     6181 tgctttatca atagttccgg cgcatccatc tcagcaggaa tcgtctttta ttttgcttcg
     6241 tcaatcatat ctggtatcct tattgcactc atccaaaaat gggagtggat caaatggaat
     6301 ccgatcagta tgttgaatct gcaaaaccaa attggaaacg aagagatcat gaagcaattg
     6361 actcatcttt ctactaatca aatgctattc ggtaatttag cttacatcgt tttgttctta
     6421 gcattaggat atctcgtatt taagcgaaaa aatgtatgat gactcatcaa aaaagaggct
     6481 gtgaaagaga cgttcagcct tattgccgat tggctgtctc ttaaacaccg tttattctga
     6541 aaaaggagct gtgacaacat ttgtgtcaca gctcctttcc tagtatgttt ttgaataaac
     6601 gacaggctca caacctctct ttatacttct tttatacttc tatacgtgaa gcatcccagt
     6661 ggcaaataaa accattgcaa tcactccgac catcgtcaca cctataagta aaatcttatc
     6721 taagtggata tgttgtacag gctcgttttg ctccttacgt gctttgaggt aaaaataaat
     6781 gcctggcaag aaaagaacaa gaacaagcat tacttcatta aaggctacga ctagcatagc
     6841 ccatacttga aaggcacttg ctaaaagccc tactaaaaat tggacccatt ctttccgctg
     6901 ataagaaagt ttcatttggt acaatcctaa gaaaagccac gtgaagaaaa tagctgctgt
     6961 accaagtgtg taagcaaagt tataagcact gtcagtgaat aataatgaaa gtaaaaagac
     7021 attcgtacat aacgtcgtca atacgatagc ataagttggt gccccatttt tgttgagttt
     7081 tccccatgct ttaggcaaaa gtccgtcttc agccatgttt cccattgttt caatcggtaa
     7141 gatcgtccat gagagccaaa caccaactaa agaaatgatc aaaccgccgt tgatcacgac
     7201 tgctccccat ttaccaacca tcatttcaaa cacatagccc atagcaggct gcgccttgat
     7261 cgtttgcagc tgattcatcg tcattgtccc atatggaagc atggaaacca agacgtaaat
     7321 aataatcaag ccgaatatcc ctagaatcgt tgctttccca acatctgatt gtttttttgc
     7381 tcgtgaacct acgacagagg ctccttcaat accaacaaat acccaaagca tcaccatgat
     7441 gcaactcaat acttgactgg aaaacgggat cggctgtcca ttcgcattca atataatgtc
     7501 gctagaaaag ttatcaatga attgttggat cgaaaacgtg ctgatagcta ctacaataaa
     7561 taaaaataat ggcactaatt tacagaatgt gacgatagca ttgacgaaac tagcattttc
     7621 gactccccga ttcactagcc atgcatatcc ccataaaaag actgtactca tcacgatcga
     7681 tgccagattt tgcccacctt caaatactgg gaagaaatat cctaatgcgc tcatcaacaa
     7741 tgttgcaaaa gctacgttgc ctaaccagca tgtgagccag taaccccaac cgctgataaa
     7801 accagcgaaa cggccaaaac cttcttctgc gtaactaaag atcccgcctt ttaaatctgg
     7861 acgtttttta ttgatattat tgatcgacaa ggagagcatc aacattgctg ttcctacgag
     7921 tacccaactg aaaatagctg ggccgccagc cgatccggag atgtccgtca taataccaaa
     7981 tacgccacct ccaatcgctg aaccaattac taaggcagca agtggaatta aaccaattcc
     8041 ttttttctgt tgctctgtat ccatgtaagc atcctccaaa ataaaaccgc ttttatcaaa
     8101 aagtataaca aacctttttt atttttactt aattaataaa aactttaata gaaatataat
     8161 tctaccggct tttttctcat tatacaagca aagcgcctag tccctttcca aattacacca
     8221 ccaaaaaact taaaaaagat gctatactaa aaggaaagaa ttggaggatg atccatgaac
     8281 attttatata tatcgatttc aggaaatacg cgtgcctttg ccaaacatct agcagaatat
     8341 gctgaaaaaa tgcatgcaga agatcctgtc aatcctgaag ttactttaaa agaaatccat
     8401 gaaaatagtg attttacgaa agaaacagaa ccatttttta ctttcgttcc tacttattta
     8461 gacggcggaa atggtttaga taacggtgat acagaaatct tgaccgaaac gatgcgtgaa
     8521 tatctggaac atgaagacaa tcataaactt tgtcttggtg tagtcggtag cggaaataaa
     8581 aacttcaata atcagtattg tctaacagcc aaacaatatg cacaaacatt tggctttcct
     8641 ttcttggcag attatgaatt acggggaaca ccatctgatg tgacacgaat ttatcagatt
     8701 ttagcagaaa acaataaata ataaaaaaga agaagctgaa aaatagattt cagtttcttc
     8761 tttcgtttct attgtttcta tcatattaag ctttgacatc catcacaggt gcttttgctt
     8821 ttgctttacc tgttttttcc aagacaaagt tttttacacg atccatattg gttaccaaga
     8881 cgatcatgga tgtttctttg cctgctgctt tgatcgcttc taagtcagct tctgctacaa
     8941 gcgtatcagg tgtcacttgc tgcccttctg ttactttcac atcaaatggc gtaccgccta
     9001 agtcaactgt gttgattccc atatgaagca agatttctaa accatttggc gttgtgatcc
     9061 caatagcgtg tttcgttggg aagacagagc tgactacccc agtaactgga gaatagatat
     9121 gtccgttttc tggttctaca aagtagcctt cacccatcat cttttgtgca aaaactggat
     9181 cacttgcttt atctaaagaa acaactgttc cgtcagcaac tgatgagagg atagtcacac
     9241 tttcagctga agtttgaggt actacttttt caggcacttc atttactgtg gctgtttcag
     9301 tcgctaattc ttctttttct aaacggtctt taggaatacc gaagaagaat gtagcaacga
     9361 agccaccagc ataagctgcc aataatccaa ggatgtagcc taaccattta ccgtcagaga
     9421 tcaatgggat caaagcggct cctgaaggtc caatagcgat agccccgaca tttccgatca
     9481 ttccgatcac tgccccgccg atacctccac cgatacaagc agtaataaat gggcgtccta
     9541 gaggtaatgt tactccgtaa atcaatggtt cgccgattcc taggatcccg actggtaaag
     9601 ctcctttgat catttctgtt aattctttat cttttttgca gcgaatccat aatgccaatg
     9661 ctgcacccac ttgtcctgca ccagccatcg caagaattgg aagcaatagc gtacgaccag
     9721 tttcatcaat catttgcaca tggattggag tcaaaatttg atgcagtccg aacataacca
     9781 tcggcaagaa taatgtgcct agaacgaagc cggcaaatgc tccaccaacg ccaagtaccc
     9841 agttgatccc accgaccatg ccatcagaaa tgaaacctgc taaaggcatg atgaagaaaa
     9901 tttcaatcag accgatggca atcaatgaaa gagttggtgt aacgatgata tctaccgcat
     9961 caggtactac tttatgcagt tttttctcaa cgattgacaa tagccaaaca gcgaagatga
    10021 cacctaggat cccaccttgt ccagctgcaa gagcttcacc agtaaataag tttttcagtg
    10081 gtgcttccgg attcatccct gtcagcatca caacggcccc gataactcca ccaagagttg
    10141 gtgttgcacc aaaaacttgt gcagcattga tacctgtata gataactagg tagctaaaca
    10201 tcccgttttt caagatgttc atcacatcga tatattgttg ccatgttgct gcatcaagtg
    10261 ttccggctgt gactaagttt gctaaaactg cggcaatccc tgccacgatc ccagtaccga
    10321 caaatgcagg aatcattggc acaaagatat tagaaatgtc ttttaatacc gatttgattt
    10381 tagatggttt ttgtttcgat ttttgtgccg ctttcatttc ttgtgcccgt tcgtttacac
    10441 ggtcttttcc agatgctgat gaatttgctg tagctgtact tacagaagaa agtttttctc
    10501 ctaatttcac gcctgccaaa tcaaccattt gctgtgctac tttgttcact ttcccaggtc
    10561 cgatgatgac ttgaagctgt tcatcttcta ctacaccaag cactcctgga atcgctttta
    10621 attcagagac atttacaaga tcatcattga tgatcttcat acgcacacga gtcatgcagt
    10681 ggacaatgct ttctacattg cccatgccgc ctgccccttt ataaatttgt tttgccagtt
    10741 cttccaaagt cagttcgttt tttgccatgg tcttttcccc ctcataaggt cccttttaca
    10801 aatccgtttg cttcagctaa ttttgcttga gcttcctctt tatcactatt tgtcaaaatc
    10861 atgacgatag ccagcttaac attttgatct gcttcttcaa agtacttttc agctgtttcg
    10921 taatcagctt ctgtcgcttg catgatgatt cgttttgagc gttcgattag tttttcattt
    10981 gttggtttga catcgaccat caagttattg aatactttac ctataccaat catactgata
    11041 gtagaaatca tattcaagat aagtttttgt gctgttcctg acttcaatcg agtagagcct
    11101 gtcaaaaatt ctggacctgc atcgacttcg attgggaaat ccgcgtgttt actgatttcg
    11161 gcttgtttat tgcaagaaat cgttcctgtg acagcaccga tcgattttgc gtaatctaat
    11221 ccaccgatca catatggtgt acgaccgcta gctgcgatac caataactat atcatttttt
    11281 gtcaaattca gcttgattag atcttcttgc cctagctcat aagaatcttc ggccccttcg
    11341 acagctacag tcattgcttc ttctccacca gcaattagac ctatgaccat ttccggttct
    11401 acaccgaatg tcggtacgca ttcagcagca tctaacacac ctagtcttcc acttgttccg
    11461 gctcccatat aaatcagacg tccacctttt ttaaaggact caatcgtgtt agcaatcact
    11521 ggttcgatac ttgtcagttc tttttcaacc gcagcggcca ctttctgatc ttctttgttc
    11581 attagttgaa cggcttgaga aatactcatc tcatctaatc caaaagtttc ttggtttctg
    11641 cgttccgttg ttaggttctc taaattgatt ttgctcattt ttatcttcct tcttttctta
    11701 taaattgata gttcatacca gctttgattt ggttgatcaa tggtagctct tctttgataa
    11761 ctttagctac tacattcact ttttcatccg ctggcaaatc atatttggtt aattggatct
    11821 cacccatata gcgcaaatac ttttcgttat ctaaagtaat agaccctttc atacgcgtag
    11881 ctgtatttct tgctgggata ggcggaatct ctcgaaaacg tgcatctacg cttcgtatga
    11941 catctcttgc atcgtcttgc cgattcgtat gcttccctaa tatatattcg taaaattctt
    12001 catctacagg ttccgtatgg agcaagactg tctgttcttt ttggaaagaa gcaaattgct
    12061 cctgaacgtt ttctgacaga cctgcatctc cgatgtaaac aatatccgtg ttacttgcta
    12121 atagatctag agcgcctgca agaggatgga tccctcgatg ttcttccaaa gttggcaatc
    12181 cttgatatag tgggcctctc aactctgtat ctcctggtac aaaaccttgg acaaggagac
    12241 cttgaatttt gagccattga ttttttttct gataccattc tttgtccagc cctgtttcgg
    12301 gacgtggata ataattatgc caagcttcaa tctgtgagaa atcagcttct gcttctttca
    12361 attcatcgat atctttaggc gtgatcgtgc tagcattcaa actgattttc aattgatgtg
    12421 accagtcggc aatctgctga ttggaaatat gataatccat tcttaaacct gttacaccaa
    12481 ttgctttcaa aggcgttaga tcttctaatg aaaaacccgc tcgttccaat gcctctcctg
    12541 aaatatcgac catcaattct agtttattct ttttcgcttc gcttcctagt tcgatcaatc
    12601 gcttttgata tgccgaaaca tcatcttctg gtatatgcat ggaagtgaaa ataccgacaa
    12661 agccattgga tgccatttgg cggatatact gtttcttctc ttctgacaaa gaatcgttca
    12721 taaatattga aaagccaaac atattatccc tctttttctg tatccgtctt catttctatg
    12781 actagagtat atatctaaag aaacaaaatt tcaatacttt tattaaaata attaaatttc
    12841 atttctaaag gttgagttta tattatcaaa agaattgtca taacctaaaa aaggcactgc
    12901 taagcaaaag cagtgccttg aaatatttta tttaaataac acaaaaacat aattactcat
    12961 aagaaaccaa ttctgtatta tctaccatga atgtaaagaa cccctcatta ggcatcggat
    13021 gaaaataagc attgatcact cttatcaagg cattatggca aactaacaga tagacatttt
    13081 cttcgtcttc gatgcattct ttcaataaag gaacgatccg tgcatacaca tctaagactg
    13141 actccccatt tgggaatcgt acagcgaacg ctaaacgtgc tttttgaaag ttctcatctt
    13201 tgcttggcag tccatcataa tccccaaaat ccatttctac caatcgttca tcaacggtca
    13261 gcggcaagct gagacgatca gcaactgctt gtgctgtatc tcttgctcgt tgaagagggg
    13321 aatggatgat tttcgtgatt ggaacaggca aatctactac cttttctgct agtttctgtg
    13381 cctgcatctg tcctttttca gtcagcggca catctgcccg tccgcaaact aatccttgtg
    13441 cgttccaagt tgtttctccg tgtctcgtta cataaagcat gtgattctct ccttcgttgg
    13501 caaagttccc cttaaccata ccttgttgtt cttgagatta caagggttag attctctcaa
    13561 gagaaataat ctcagtagat actttatcca ggaaggtttg atcatgttcc actagtaaca
    13621 tggcaggttt cacgttcaag atcagttgtt ctaattgttc ttgattgaag acatccaaat
    13681 aattcaatgg ttcatcccat gtatatagtt cagctggctg tgataaagat ttagccaatt
    13741 ccactttttt ccgttggccc atactcatct gctcgatctt gttatgaaaa acatctcttt
    13801 ccatcccaag ctttcggagg ttgttcaaaa atgcttggta gtctacttgg tttttctctg
    13861 caaattccgc caacgttcct cgattgtctt cataattttg gcttgcataa ctaatgctca
    13921 gatgttttgg ctggtatttt tctcctataa ccttgccgtt gaatgccccc agaagataat
    13981 ggataatgga tgactttcct gcaccgttag gacctgaaat cgccacccgc tgatgaggct
    14041 cgattgtaaa atgaattggc tcgaataaca gattttcata ccctaattga agatcttcta
    14101 cgcttaaaag tcgcttatgg tgagacgctt ggctattcat cgtcaaagaa tcgatatatt
    14161 cgatatcttt taatagtttt tctttctcgc tgatctgggt ctccatccga ttcacgattg
    14221 ctttggatcg tttcatcgtc cgtgcagcat cagcacccac tgctccttta ttcactcgtc
    14281 tagattcagt atcgatgaat ccgacttgtt tctttgtttt atctccttct cgggaacgag
    14341 accattcggc tttttcagct gctgttttct ttagcctact gacttctttt ttcaattttt
    14401 cattttgagc catttcaaat tcatcacgaa gttttttctg ttcttcatag atagagaaat
    14461 tcccttgata aagttccagt tgactttttt cgattgctaa aacatggtcc actacttcat
    14521 cgataaatcc ccggtcatgg ctgaccacga tgaagccttg ttttttcttt ttcaaataag
    14581 ccgctacttg ttttctacca gagatatcca aatgattcgt tggttcatcg attaacggga
    14641 aatgagtgtc atccacaaat aaaagtgcca gtaggacttt tgtcttctct ccccccgata
    14701 gtgtgctgaa ttctctccat aagatttcag gatctgtttg catcaattgg agctctcttt
    14761 cgatttccca tatctcaaaa tccgtaatat catttaacac gtaataggtt aaacgttctt
    14821 tatcttttgt ctgttgcggg aaataggcaa attcttgctg atggattact tgcccttgat
    14881 aaggtagttt gttttgtaga atattcagta aagttgtctt tcctcgaccg tttcgtccga
    14941 taagtcctag tttccattgt gtgtcaaaat ttagatttgc ttgttcaaat aataatgtgc
    15001 cttggctgtc gtagccgaat gtcagatttt ttatttcgat tttagacatt ttccatcacc
    15061 tcgtaaaaaa agaccagtcc tattttcaga actggctaaa gaggatgcaa gggttggata
    15121 cacacaaaaa gacccataca tcgaaaaaac caagccaatc ccgaaaatct gcacactgct
    15181 tccttgtctc cacaaggtac agtctgaatg cagctttcat caatcgactt aatttttcaa
    15241 tgcacggatc cctcactttg tcctatattt gaaattacga tagcataaag aaaatgattt
    15301 tgtcaactac ttcgggagga tggtgaaaaa tctgctaagt gtctgtccta agatttttgt
    15361 tttttctaat gtttgcctgt gttccattgc ttcttctaaa cctattggac ccaaatgaat
    15421 acttaagaca attgggagaa aatcagaaag tccttctagc tcttggtctc tacttccgca
    15481 aatagcgatt attggttttc gatatttttt tgcaagcact gctacaccgt atggaacttt
    15541 tccgtggatc gtttgctgat cgatccgccc ttctcctgta atgatgacat ctgcttgttc
    15601 gatctttttt tccaatccag tgatttggct gactaaatcg aaaccagaaa ccatcttcgc
    15661 tcccaacaaa aaaagggctc caccaatacc gcctgctgct ccacttcctg gaatatcatc
    15721 taaatccacc tcccagaaat cttttatttg ctgagcaatc tttcctgctt gtttatctaa
    15781 gaaagctagc gtcgtatcta ttcctccttt ttgtcttcca aattgccaag cagctccttg
    15841 ttgacctgta tatggattgg taacatctgc agcgataatc aattttactt tagaaacaag
    15901 aacgtttctt cgatcaaaat gaagtttttt agtagataat aaaggatttc cttctgtgta
    15961 cccttctaaa gaagcaccca aggattggag taatcctaga ccaccatcgc tacacccgct
    16021 tcctcctaaa gtgacaataa tttgttctac ttcttgaagc agtgcatctt taataagttc
    16081 tcctaaggca taactggaag caagtcggat agtttgatcc gttggtttat caagtagatc
    16141 taaccccaat gcttgtgcag attcgatgac tgcggtgttt tttccttgcc aagttgtcaa
    16201 taaatagctg atttttcgct gacggaataa gagatccatc ccatcaatgg tacgccatgt
    16261 tcccctgagc gcgtaataga ttgcttctgc agtattttct ccgccatctg caatcggcac
    16321 attgattttt cttcctttac gatcttccca gccatttaga actgcctgat ttagttcaaa
    16381 tgaagaagca cttcctttaa atgaatcaat tgccgccaaa atatccatct gtatccctca
    16441 ctttcttctt aagtaagaca ctttttttca tttttttcaa acaaaacaat gctattttct
    16501 tccatttttc tatatactaa aagggaactc attgaataaa ggagagaaaa aacatgaaaa
    16561 tcgcaatagc tggggccgga gcaatgggca gccgaatcgg attgatgcta catcaaagcg
    16621 gaaatgaggt cctcttgatc gatcgttggc ctgctcacat tgaagccatt cgtacaaatg
    16681 ggttgattgc tgactttaac ggtaaagaag tggtcgcaaa actgccaatc tattcaccag
    16741 aagaaatcat cgaaagcaac gaacacgtgg acttgatcgt agcattaaca aaagccaatc
    16801 aattagatga tatgttttgt tcgatccaat ccattattac tgacaatact tatgttttgt
    16861 gtttactaaa tggattaggt catgaagatg tactggaaaa atatgtgcca aagaaaaata
    16921 tcttatttgg gatcacgatg tggacagctg gtctagctgg tcccggaaaa gtcacattgt
    16981 taggtgatgg agaaatcgaa ctagagaact tggaaccaga aggcgaagca tttacaaaaa
    17041 aagttgtaga agtctttcaa gaagcaaatt taaatccgat ctatagccat aacgttcgtt
    17101 attccatttg gcgaaaagct tgtgtaaatg gaacattgaa tggtttatgc acgattttgg
    17161 attgtaatat tgctgaacta ggcgcgcaaa aagcggcaga gagcatggtt cgaacaatcg
    17221 tatctgaatt tgcttctatc gcagcaaaag aaggaatcat actagatcaa gaagaagtgt
    17281 accaacatat tgcttctact tatgatcctg acaacatcgg gttgcattac ccatctatgt
    17341 accaagacct gatcaaaaac catcgtttga ctgaaatcga ctacatcaat ggtgccattt
    17401 ggcgaaaagg acaaaaatat gatattgcta caccatattg tgcctttttg acccaattag
    17461 tccatgcaaa agaaggaatc cttggcgcag aataaaaggc tctctgatga gaatcagctg
    17521 aatcagcagc tggttctctt tttttaagcc caataaaaga tcaaaaagaa aacacaactc
    17581 tcatgatgcc ataagaattg tgcttgtttg tttcagtaca taatctcttc aaaatgtagg
    17641 ttttttattt gttttcaaca gaggagaggt catattcgta ttgctcataa atagtggaat
    17701 cgttagtgat aaatcgtttt gcccaatttt ctgcatcata aaattccaat agctctgata
    17761 ggattggctt aaatggtgta tgcagaaatt ccggtaattc atctaaatca cgttctcgaa
    17821 gcaaaaaaat attttcagga cgataaaaat gataatgagc aatcgcttga ttatccgtca
    17881 ctagtttctt ttgaaaatac atggattcaa atgtccgcaa agtcactcct tgctgaccct
    17941 cccgcaacag ttccagaatc ccgcgcgatt cccctgtttt tttcaggtac tcttcataag
    18001 taagatatcc aggttgttca ttccctcgat gtggaagaat gaaatagttg gtcgtaacat
    18061 ctctttttat aaattcctgc ttatagcttt ctgctcgttc ttttctccca ttattcgttg
    18121 caccaaaaaa tagatccgtc tttggttcgc tagaagaaaa attcatatgt ttcgaataaa
    18181 atgaactatt atgtttcagc ccaagttctt ttgcttccaa aaaatcaaaa tgataaatct
    18241 catcgattgc cggttcatct tgtgcctgct tcagcatcgc ctcgtgttct gctttgaagt
    18301 gattccaaaa aaataacaca atttttgcat tggtaaatgg gcgcacgcgt cgagccatct
    18361 ccatatcaaa tccataatct aaaaaaacga tcagctgata atttgccaac tgttgtctag
    18421 ctgatttaat gaattccgac tgtagtaaat cttttccgga ccaaacatct aaagagcgga
    18481 tggaaggcaa aaaataaagt gatttatttt ttgcaactag cagtacgtca tttttgttca
    18541 tcttttccgc ctcactttct ctgtcacttt ttcctatgat agcctgttta aatagtaaaa
    18601 agccagtaaa acgtttccta tatttgtatg aaaatttaac atttgtatga aaatatgatg
    18661 aaataaatca ttcttctatt aaaattcgcc taaaagtttt ctgaatagtt gtaattgtct
    18721 gaaaatttgt tatcattagt cattgttaaa tattgctatt tattcttaat ataaattttg
    18781 attgtgaatt taaaagaaag aggtcattta tggaagataa aaaattgaaa aaacaactta
    18841 gctccagaca tatcaccatg ttagctttag gtggagcaat cggtgcaggt ctatttaaag
    18901 gaagtggcga agcgattggg atcgccggcc cttctgtatt gattgctttt ttgattggtg
    18961 ggctgatttt atatatcgtg atgaaaggtc tgggaaaaat cgttttaagc ggtggcgaca
    19021 cgcatcacgg attatctggt ttgatacgcc cttatctagg tgctcattct gctgatttta
    19081 ctgattgggt ctactggtct atgtggatta tcaatatcat cgctgaagct gttgcagctg
    19141 cttccttttt gcagttgtgg ttcccgcaca ttccagcctg ggtatttgtt tttatgttgg
    19201 ctgttttaac tacgatcatc aatctttatt cggtacggtt attcgcagaa acagaatatt
    19261 ggctggcttt tgctaaaatc agtgtcatta tcttactgat catttttgct acttatcttg
    19321 tcggtcagca aatgttgggc acaggcgttt tccctaccct tcagcaattg actgaccatg
    19381 gtgggtttac tcctcatggt atgaagggaa tcatcagttc tttgcttgta gttatctatt
    19441 cttacggcgg ttctgaactg attgctatca cggtaagcga agcagatgat cctaaacgtg
    19501 cgattcctaa agcaatcaaa ggtgtcatgg gaagaatcat ttccttttac atcattcctt
    19561 tgtttttgct tttgattctt tttccttgga atactttagc aggcacgaat gtcagtcctt
    19621 ttgtgatggt atttgaaaaa atgaatatcc catttgcagc ggatatcgtc aattttgtga
    19681 ttgtccttgc tttgttctca tctatcaatt cgggtgtgta tgcatcttca agaattttgt
    19741 tcttccgttt gaaggatcga aaaggttcct ctagaaaact agctgtttta aacaagcatc
    19801 aagtaccgca acgtgctgtt ttattttgta ccagtgttct ttatcttggt gtagctcttt
    19861 cttattttgt cggtgacaaa ttatttggct atctggcagg ttccctttcg tatacagtat
    19921 tattgatctg gatcattatc agtgcggcat cctttgtact ggcactaaga aaaggaacat
    19981 tatgggataa aggaatcagc ttttttgcat tagctgtctt aggattgatt ttccttggta
    20041 tcctgttcac gaactccatt ggggtaactg tattgactgg cctgctttac ctgttcattt
    20101 atttcagtta tcaaaaaaag aatgatgcgt tcgtattgac gaatgaatga aatcgataga
    20161 aaattttatt ttatttatgt ggaatagatg gagctattga agaaaggagt tgtgacaaaa
    20221 gtcttgttgc aactcctttt tccgaataaa cagtctcatg acctcttggt aaacggtgtt
    20281 caaaaaccct ctgtgcaaaa taattgattg ttctccatgc tcatgacatg atagaagaaa
    20341 ggaatgaaaa aaaggagttt gttttaaatg aaaaagtatt ggaagttttt gttgatgatc
    20401 ggtgtttcaa cggtagttat gtttggtatg atgtacttga atgtctatgc gctcgatcat
    20461 atcttcttca gtcgtacacg agtattcatg gcgttgatga tgggtgcagt catggcagtc
    20521 atcatgctgt tgttcatgtg gaaaatgtat gaaaacaaaa agataaatgt tatgatctta
    20581 gccgctagtc ttcttgtttt tgccggttcc ttgtttatgg tacgaagcca aactactgtc
    20641 aacgacacag cttggatgaa ggccatgatt cctcatcatt ctatcgctat cctggcaagt
    20701 aaacgagctg atttaaaaga tcctgaagta aaaaaattag cggaagaaat cattcaggca
    20761 caagaagaag agatcgacaa aatgaaaaaa atgatcaaag aaatggaatg accattatcc
    20821 gtccaaataa aaaaagagga tgttccagaa gttatcagct tcaagaaata agaagaagct
    20881 acttctggaa cgccgtgaat aaggggttgt gaaaaagctg ccctgcatca agtaataaga
    20941 acagccaaac aaaaatagct tctcctattt ttcgtttggt taggcttatt accgcagatg
    21001 caagcttttg aacaccgttt attaagaggt tgtaaaatca gcgttctgac ctgagtatta
    21061 gagagacaaa tggaaaaata gttcctcata ttttgccatt ttttgagcta ataccgaggg
    21121 tcagctgatt gaacaccgtt tatcgagagg ttgtgaaaaa gtcgcttagt ttcgcgtatt
    21181 aagaaaaatt ttgtgaaatg gctttccaca tttttgtata aatttggctt aataccgaga
    21241 aactggcttt tgaataccgt ttattcgtaa aagggattgt gacaaaagtc ttgtcacaat
    21301 ccctttttat gtttttattg ttctaattgc gctgtcttgc ttactgcttc ttgcggtttc
    21361 acaaattttt ttgagattcc ccaacctgtg aaggcactga ccatcgaaaa cacaggagaa
    21421 agcaaactga aaaatacgaa tggcaagtaa gtgagtgtag gaataccaaa tgtacttgct
    21481 acgaaacttc cggcaattcc ccaaggcact aagtagttga ttactgttcc accatcttct
    21541 agcgttctac ctaatacagt tggatcaaat cctgcttttt cataagtttc tttaaatgca
    21601 ttaccaggca gaataacaga taggaattgt tcaccaataa aaatatttac tccgatactt
    21661 gtcatcaatg ttgtaaaaat caaatttggt gtgttcgtca tctttcttga tactcgttcc
    21721 atcacggttg agatcaagcc aaattcgatc aacaatccac ctaatgacaa agttagaata
    21781 atcaatgaca cagtaggcat catactttcg attcctccgc ggctaagcag tgaatcgatt
    21841 tgttggtttc cagtatttga aacaaaccct gattcgatga ccccagccaa cttcgttaag
    21901 gaagtttgtg ggttttgaat aaaaatcatt cctactgcta cagcgatatt caacaaaatc
    21961 gttaaaattg ccggcacctt tttccatgca cacagtaaca ttaaaaataa aggaatcagt
    22021 gaccagattg aaatagtaaa gtttgcttct agaacatcag tcacttcacg aatagatgaa
    22081 agacttgcgg ctttacttgt gtgtcctaaa atcatgaata aaaagaaaga aacaacaaat
    22141 gccgggacag ttgaccacat catatgcttg atgtgtttga acaaatcggc atcaacaata
    22201 gctgctgcta gatttgttga ttcggataat ggagacatct tatctccaaa aacggcacct
    22261 gaaacaatag ctccaacaac cagcgctgga tttaagccta gtgttgtccc aataccgaaa
    22321 aatgcaatcc caatcgttga cataacagta aaggcactcc ctacagcgct acctacgatt
    22381 gcacacacaa caaatacaga agggacaaac cactggacac tgatcaattt gaacccaatg
    22441 accattagag ttggaatgat ccctgcttgt atccatacag caatcaatga accgactaaa
    22501 ataaaaataa agattgggat gattcctggt ttgatgccat tgatgattcc ttcgtgcagc
    22561 caatctgtcg ggtatctttt tatcactgca tacaacatga ccattccgat cgctgctaag
    22621 atcgtgatat taggtgagac ctttaatccg acaacgccaa tcgcaatgcc taaaacaata
    22681 accattaata caagtgttgc ttctaaaaaa cttggtgttc ctttttttcc catgataatc
    22741 tctccttttt tagttgttct tatgttctaa aaaagttaag atatcggatt actccgccgc
    22801 aattcatccg cgattcctcc tttataaaaa taaaaagacc tgttttaaac taaggacgaa
    22861 tgactccgcg gtaccacctt aattaaaaac agatctgttt ttcactttca aacttttatt
    22921 caaatgtatg gactttccat gtaattcgtc aattaggctt gatcggttca cagcgaccac
    22981 cgactttctg aacaaaacac ctaaccgcta ctgcgtgaaa agttgacttg tacgaagttt
    23041 accttccata agatcatttg tcaatgaaaa agaaaatatt ttttcatttt tctgaaaatt
    23101 atttttataa ctttcacaca caagtttatt ttttactaga aagtagcgcc ctcttgtttt
    23161 tttcttgcta ctatttcttg atgttcttgc ttgattcttt ctaatagctt tggctctttc
    23221 aacagatcaa gagctgtatt ggcgagtagt tctgctgcaa tcgcaataga agcaaggcct
    23281 ttgtcacttt tagcagcagc tttgaattct tctgtgtgtc cggcaatata tgtctcagag
    23341 attgctaccg taggctggat tgttggtatg acttggctga catttcctac atcagaagaa
    23401 ccgagacttg ttctttgttc cgtttctatt tcttcatctg gaacacctgc ttcttttgca
    23461 tgctggaaga ataagtcatc aaaagaagga gtaacgatga catcatcaac tgcattttgg
    23521 aacaatccaa actcaaaagt cgtgcccgta cttatcgcag cacctcgtac gatattttcc
    23581 actttttcat atactttatc taatgtttgc cgatttcctg cacgtaaata gaaacgtcca
    23641 gcagcgtaat ctggaatgac gttggcagct actcctccgt ctgtaatgat cccatggata
    23701 ttgacatctt ttggcaattg caagcgtaga gcgttgattc cattaaacac ttggatcaat
    23761 gcttccaaag catttactcc tttttcaggt gctgctgctg catgggaagc gacaccgtaa
    23821 aatttgatgt cgactggatc attcgctaaa gattctgtcg ttttcccata aagaaaagct
    23881 ggatggacgc ataacgctac atcgacatcc tcaaaaaaac cttctcgaac aaagctgcct
    23941 ttagctgatc cgttctcgcc cccttcttcc cctggggtgc catatacacg gatttctcca
    24001 ccagtttcat caatgaattg cttcactatg ctggcggcta atacggagta agtaccaaac
    24061 aaattatgcc cgcatgcatg accaatacct ggtaaagcat cgaattctgc caaaaaggca
    24121 attgtgggac cgggtttatt ggaaatataa cgtgcatcaa aggctgttcg gtgtccagct
    24181 acttcttttt ctacccaaaa cctttctttt tctaattgtt taatcaacgc tgctgaggaa
    24241 taaaactcat agttacttac ttcaggatgt tcgtggatat ctagagcaag tgcttgatat
    24301 tctggtaaac gatattggat tagatccttc aattggtttg ttgagattgt tgttgtcata
    24361 aaaaattcct cctatgctag tcatttttct tattttctcc cgaatgtttc ccatgcaggc
    24421 acgcttgatc cttttgatgt tttctcgatt actttgactg tgtcttctgt ttgataagcg
    24481 tctactattt tttgatacgt tttattgttt tcatcttctt ttcttgcaac gatgatattc
    24541 acataaggac gtgcatggtc atccactggt tctaagaaga tggcatcaga agcaggatta
    24601 aatccagcat ctacagctac accgctattg atcactgaaa tgtctacatc tgacaaagca
    24661 cgtgctgttt gggaagcatc taattcagaa atgtctagat tttttctatt ctcggtaata
    24721 tcagaaacag tgggcgcttg tccttttgcc ttatccactt tgatcaatcc agcagtttgc
    24781 aataacagaa gagctctgcc gccatttgta acatcattag ggattgctac ttttgctccg
    24841 tctttgatat cagctacatc cttcaaagtg ttagaataaa ttcccagcgg tgcattgaca
    24901 gtatttccga tcgatactaa atcagttccg tgttctttat tgtaattgtc taaaaagaat
    24961 tgatgttgga aagaattcaa atcaatcgat ccatctgcta aagccacatt tggttgattg
    25021 taatctgtaa atttcacgta ttccaaatta atgttctcct ttttcaaacg gtctttcacg
    25081 ttgtcccata catctgtatc ttctccaata atccctagtt ttacgatagt ttcttttttg
    25141 tcagttgtat ctgcttttcc ttggctacac cctgccaacc aaataattgc tacaaatgta
    25201 atgactacaa aacgaataat ttttttcatt ttttctcctc cactttcaat gtgtaatttt
    25261 tttaatcagt tgattactga taaattgact taaaaaaact aagattagaa tcaataatgt
    25321 agccacaatc gttacatctg tttggaatcg attataccct ctcgatatag caagattccc
    25381 taatccgcct gcacctactg ctccagccat ggtagtcagt ccgatcacgt tgatgatagt
    25441 caacgccgag acccgaatga taccaggcaa accttctaac aaatacacac gtaaaatgat
    25501 accgattgga ctggttccca tcgcttctgc cgcttcgatc accccaggat caacttctag
    25561 cagtgcgttc tcgatttgac gagcaaaaaa cggaacaact ccaaaaatca atggcactaa
    25621 agctgctttc gcaccaatcg tcgttccaac taagaaacga gtagtcaatg ctaaaagaga
    25681 aagcaagata atgaaaggga tggaacgaat gatattgatc agcttatcaa gtatttcaaa
    25741 tagcagacgg ttagctagta atccattagg gcgtgtcact actaacgtca cacctaagat
    25801 gattccaatc atcccgccaa tgaatgctgt ccaaaaagac atatatagtg tttgcttcgt
    25861 cgcttcgaca aattcatccg gaatcaaata aacattgggc aaatatgttt taaagaaatc
    25921 agtcatttta tcttcctcct tcgatcactt tgatacgact atctaaatcg tttttttgta
    25981 aaatatttat ttttacacca gatttgatca aataatccaa tgcttttttc cgctgtttgg
    26041 tttctccagc taaagtaacg atcaaactcc ctaacggcgt ttcttgtaat aactctacat
    26101 tgccatataa aatattcgct gttacttgat atttgctgta cagatgagcg attagaggtt
    26161 cgttcgtctg atttccaata taacttaatt ccaccagcca tttattggcg atctgatcgg
    26221 caaaagcaga atgggcaatg atcgtttcta atgcttggtc aagatgagta gctgttcgaa
    26281 taaaatcttt ggtcaattct tcttcaggat tgctgaagat ctggatgctg ctaccttttt
    26341 caacgattct tccatcttcc ataaccgcta ccttgttgca gatttctttg acaacttgca
    26401 tctcatgagt gatcaggacg attgttaaac ctaactggcg attcaatttt tttaacaatg
    26461 caaggatctg aagggtcgtt ttcggatcaa gtgcactggt tgcttcatca cataataata
    26521 ccttaggttc attcgccaaa gctcgtgcga ttgctactcg ttgtttttgt ccccccgaca
    26581 gttgtttagg ataagcagat gctttttctt ccaaaccgac aagttccagt agttcattca
    26641 ctttttgacg acgttcctgt ttcgattttc cagaatactt taaagaaaaa tcaacgttgt
    26701 caaaaatagt tcggctttcc attagattga aatgctgaaa gatcataccg atggcctttc
    26761 tttcttttcg caattctttg ggagagagct gactcagatt cgtctgattg acaatgactt
    26821 ctcctgctgt tggtttctgc agcaaattca tcacacgaac taacgtactt ttccctgcac
    26881 ctgagtaacc gacgattcca taaacatcgc ctttttcaat cgttaaatct acagaatcta
    26941 ctgcctgtat ctctttttct ttttgtttga aacatacaga aacatctcgc aattcaatca
    27001 ttaaaattcc tccttatcta aaaaaagcgt cctcaagaaa atgaaaatgt cattttcttg
    27061 aggacgcttt tttgcgtgtt accacctcat gttcaagctg tgttcacaca caactcctct
    27121 tcaagtacga tcatactcta gcaagatatc ggtcgcagcc gtcatcgcct tacatctatg
    27181 ctcgggatga attgctcgat ggccattttc caatagtcgt tccttgcttc ttctcagcta
    27241 ccgaagctct ctgtcaaagg ttcttctatc gtacttacca tttcaatgca aataatattc
    27301 aaaaaaaaag tccttttact aacataaact atgtcagtaa aaggacgacg tatcgcggta
    27361 ccaccttttt tcggaagttt ccttccctca agcccaacat ctctaaaaga gagattggcg
    27421 ttcgttttaa cggtcgaaca gttccgaatt tgcctactta tcgacaaatt attctccaag
    27481 gccattttca gcttttctta cttgactctt ctcaccagcc agagcctctc tgcaaagttt
    27541 ccaaacttac tttccttttc aacgaaatcg atattttgtt gttgggatta atctacatga
    27601 ggaaaactga aaagtcaagt acgaaattca aattattttt tctatattta tttttctgtt
    27661 tttttattta acatttcaat aacagttcga agatcttttc cttccgcaag aagtttcaat
    27721 ttttcctttt cttcgacggt ataatttcgg acaaaactgg aatcaggtat caaaacaacg
    27781 cctaatcctg ctcgtgaagc agctgttgct cctactaaag aatcttccac tgcaaaggct
    27841 gaactagctt ctaatcctgt tcgttccaaa gctgtcagat aaggaatcgg tgaaggcttg
    27901 ttctccgaca cttcgtctcc gaatgtaaac gtgtccaagt aatcagctaa tttaaagtga
    27961 tccaagatat ctgttgctct tttcgtaaca gtggtcgtag caatgcctag tttcatatga
    28021 cgttctttta agtctgccaa tatttctttt gcatatggtt ttaagtggat ggctccgctt
    28081 gtcagctgtt gataaaaata ttcttcacgc ttcgctcgga cttcctgtac tttttcaggg
    28141 ctacctgctg ctttcactaa aatatcaaaa ctttgtttcc agttttttcc tccccaagcg
    28201 tcaacgattt cttttgggat atggatatca aattgctgaa atccccaaag ccagccatca
    28261 cgatacgttt tttctgtgtc aactaacaaa ccatctaaat caaatattgc acctttcatc
    28321 tatacattaa tcctttccaa aacaatacac atagtttagc acgactggtc caaaaagaac
    28381 agaagcaaag aaccagcctt ctcgtttata agagttcttg gaatcgacgg atatagattt
    28441 tcttcacagc tgtcacaagc gccagataag cgatgactgt agcaaataac cataaccagt
    28501 aattgccagg aagtggcatc aagcctaatt cagcaccaaa acttgtaaaa ggaaggacag
    28561 aaccgacaat aattcctaat gtagtgataa caaacatggc aaatgttgcg ttgctttgga
    28621 taaatggtac tttcggtgta cgcagtgcat ggagcactaa ggtttgtgtc catagtgatt
    28681 cgacaaacca tcctgcatgg aataatgcga tgaaaatcac tcgctgtgaa ctatccaatg
    28741 tatggaaact tccgcccact acagcaggac aaataacaaa atacatcaac aagtaagtag
    28801 taatatcaaa tatcgaactt gttggaccaa agtaaaccat gaattcccca atactagatg
    28861 cattccattt ttttggctcg tccaaatatt ctttatccat ccgatcccat ggcaaggaaa
    28921 tgcaggaaac atcatagatc agattcaaaa acaaaagttg taaaggcagc attggtaaaa
    28981 atggcaggaa cgtacttgca atcagcacgg aaaacatatt gccaaaatta gaacttgctg
    29041 tagccttgat atatttcatg atattcccaa aagtttctcg tccagaaaga aggcctcttt
    29101 ccaaaatcgt caaatctttt tctaaaagga tcacgtctgc agattctttc gcaatatcca
    29161 cagctgtatc tacagaaata ccgatatcag ccactttcat tgcaggagcg tcattgatac
    29221 catctcctaa aaatccgaca gtatgaccat ttttacgtaa agctgtcgtc aaacgtgctt
    29281 tttgctgagg attcaatttt acgaatactt catggtcttc agctacttgt gctaattcat
    29341 gatcactcat ttccgagatt ttctcaccgg taaccaattc attgatctct agacctactt
    29401 gacggcacac tgaacgtgta actaattcgt tgtccccagt cagaactttt acagctacac
    29461 catgttcttt cagagctttc aacgcttgtt tcgttgtttc ttttggtgga tcaaggaaag
    29521 caagataacc aatcaaaacc atgtctgact catctttgac tgaaaattct ccaacgacgc
    29581 ttggattggt cttttgtgcg acaccaatca ctcgtaagcc atcttcatta agtgactgta
    29641 cttttgcaag tacggcttct tttcgttctt ttgttaatgg tgtaacagta ccgccaagat
    29701 caataaattt agaaatgttc agcatttctt caatcgcgcc ttttgtgatc atttgtgttt
    29761 taccagctgg atcagcaacc acaacactca tacgacgacg ttcaaaatca aaaggaattt
    29821 catccacttt ttgataattg atcttatctg tatctaaagt ttctgttgct tcatcaatga
    29881 tagccacatc taacaaattt ttcagccctg tttgatagta gctatttaga tacgcatggc
    29941 gcaaaacacg atcatcttca tttccttcac aatccatatg atattcaaga atgattttat
    30001 cttgtgtcaa tgtccccgtt ttatccgtac ataacacatc gattgcacca aagttctgga
    30061 tggcattcaa atttttgatc actgttcctt ttttggccat cacacttgct cctttgacca
    30121 aattcgtcgt aacgatcatt ggaagcattt caggtgttag accaactgct acagatagtc
    30181 caaaaagaaa agcttctagc caatcccctt ttgtaagccc attgatgacg ataactgctg
    30241 gagccatcaa tgacataaat ttgatcagta gcatggatgt tttatggatg cctaattcaa
    30301 agcttgattg aattggtttt tcagatagcg tttctgctac atgaccaaat agtgtttcat
    30361 ttccaacaga aacaatgatt ccttccgcac ttccactgat gacattactt cccataaaga
    30421 caagattttc gtaacttgtt tcgctttgct gatcgtcttc acatactttc gcatgctttt
    30481 ctactggata gctttcccct gtcaatgctg cttgggaaat gaataaatcc tttgcggaca
    30541 ataaacgcat atccgctggt atcatatccc ctgctgaaag ccgaacaaga tctccacaga
    30601 cgatttcttc cataggaatc tctgtgtact ttccattacg ttttacagct gccgttactt
    30661 tcacaagtga tttcagtttt tcagccgctt gattggaacg tacggactga atcaacgtca
    30721 ttgtcccgct gacgataacc atagtgaaga taatggctac accgaaaaga tctttatctc
    30781 caggagctgc taatacatat tccgtgatga atgaaataat tcctaatgcg atcaacacta
    30841 atgtaaatgg cgtgatgtat gctttcaata tttctacaat gaatggtgtt tttttgccat
    30901 atgaaatcgc attttcaccg taattttctc ttgaacgttc gatttgttct tctgtcaatc
    30961 catttgctga tgcatggaat agttcaaata attcttgttt cgtttttcca gcaaaagctg
    31021 catagatttt ttgtgtatct ttcttttttt ccatctcaat ctctcctttg tgtccaaaag
    31081 acagctgttc tcgctgcttt tggtctttag ctattcatcg gatcgataga gaaaaaactg
    31141 aaaggggctg agaacagata gataatagcc ctggcctttc ctctagatcc ttgtttttcg
    31201 aatgactgtc atccatttgt tctcatctcc tttactaagt aattttaatt catgacttcc
    31261 aatcattttt cgaatagtta tttttccaat tagaccacct cctttttatt tttcaccgat
    31321 ggggatttgc ggcaacaaaa aaacctctcg ccaaatactc agcgaaaggt ttgaaaaagc
    31381 atattaaaaa aagcttctca gatctcatcg ttgagttttg acactgtaca acgtaaaaag
    31441 cagatacctg cttttagcta ccttatgaaa agccttatct cgacaattcc tgttctaccc
    31501 attggcgtct ctcgacgttt ttgggcagta gcctatgttt gtataggagc ctcacctaac
    31561 aaatcttatt cggttttcct tcgttaatat aacaactaaa aaagaggatg tcaaatcaat
    31621 tttccatgtt tttcatactt tttttaaaat acacacgtta tattcctgtc tcacgcggtt
    31681 ttatttgtct cattttccac ctcccgctat aatagaaata tagtaggaaa cgaggtgctg
    31741 ttatggaaag aacacttgtt attatcaagc cagacggcgt gagacgccat ttagtcggaa
    31801 gcatcatcca acgttttgaa gcaaagggac tggccattgc tgaaatgaag tttgaaacta
    31861 tgactcctga attagcaaaa gaacattatc agcacttgac tgagcgcagt ttttttgatg
    31921 aactgatcga ttacatgact tccggtcctg tcgtttatct tgtgctggtc ggcgaggaag
    31981 tgatcgatat cgtccgtaag atggttggag caacaaaagc tgccgatgct gttccaggta
    32041 cgatccgagg agactacgcg ctgccgggga ctgaaaacat cattcacgct tctgattccc
    32101 gtgatgctgc ggtaaaagag atcgcccgct ttttcccgcc attcgatata aacaaaccgt
    32161 ctgaaaaagc ggcgaagtga tacaaattct aaactgctgg tctagttgct gggttcagca
    32221 atggccagca gttttttctc taaggacctc cgttgattct ttcctaacat atattcgagc
    32281 actgtgaaga attcttggta gtctttcgtc ttttcttccc ctaaagcagc agaaatcaat
    32341 ctttgataat aatctgccat caatgtttca tagatcactg tacttctttc tttatgtgct
    32401 aagctccgtt tcatatcttc attcacacta cgtgcctttt tcaaccgacc attttctata
    32461 taaaacgaag tcaggttcat taaaaaagta aagagatact gctcatgatt gttcaactgt
    32521 gctaatcgtc gcattctttt tttgattttc aaataattgt tctcaatgaa ccgatcactg
    32581 aatatccaag gaaaatcagc tagaatagat agttcaaatt gaccatagtc tggtatctgc
    32641 atcaagtatt cttcaatgat tcggcattca taacgtaaaa attcaaattc tgtctcagat
    32701 acttctgttt tttcgaccat gtgaagattc aaaaccaaat aatacagatc ttctgttttc
    32761 ttaaaatgcc tacggttatt gctgatctct ttataaggat caaagctgtc ttcttgtacc
    32821 cgatctagca ttcgtctttt tttctttaac tttattggtt catccagctg gtttcgcatc
    32881 cattcatatt ctgcaagtgt catgttcatt cgctctaaaa atgatctcaa tgttacaaat
    32941 gacatttttt tcactgaatt tgtctcaatc ccgactagag aagtcttaaa acagattcct
    33001 tccgctaatt gggactgtgt caacttcttg ttgactcgaa gtttcttgat caattcacca
    33061 tcatacataa tagttctcct ttttaattta aatttttcca cattcaataa atagatacgt
    33121 taaaaaggaa aaatacacga aaaaaataaa aaaatgtttt tttcataaaa aataccaacc
    33181 ttcaagcatt agaatgttgt ctaactactg aaggttggtt tgattgttta ttgattattt
    33241 ttttgctgtt caacgtctgt tcttgttact ggatataagt tggttgttgt tttattacca
    33301 ttttttgaaa agacacttgt cgaatgttcg cctttttgct tttcgattgc ttcattacgt
    33361 tccgcttcat ttttatagtc atattttttc ggatcaatcg tctcaaatcc gtttggcaca
    33421 taaaaccgca agagattttc ctgattcagc ttatctgaaa gacttaacgc agtcttgact
    33481 ttttcctgat ctttggagat ttcctcatcg attcctgctg ctttcgtatc gatgatttct
    33541 cctgtctgat tattgtaagc ttttcctcct aggacggtgt atttgggtgt cacaaagttc
    33601 ccatttcgaa aagcaactac ttgatcatgc tgagaagata ataagtctgt accaaaatga
    33661 agataatctt tatcatcgat tcccaataga tgtagtaatg tcggcaaaac atcgatttct
    33721 ccaccgtatt catgattgac tgtcccttta gtataaccag gcatatggat catcaatggc
    33781 actcgttgca tctgtgcgtt gtcaaattca tcccatgtat ctggatcttt accaagagcg
    33841 cttgctaaat ctttattatc tgtattcgag atcccaaaat gatccccgta aatcacaaag
    33901 attgaatttt ggtaaatacc agattttttc aaatacgtaa aaaattgttc caatgattga
    33961 tccagataat gtgccgttcg aacataatta tctactgtgc tattgccagt attcaaagat
    34021 ggaaagtcga aattcttgtc atccgtataa taaggcgtgt ggttggttac ggaaagaaat
    34081 ttcgtgtaaa acggctgttg tagatgctca agatattggg cagattctct gaataaatct
    34141 ttatctaata tcccataacc taacgtttcg tcggattcat caaaataaga tcgatcaaag
    34201 aaattgtcat aacctaagtt tttatataca tgatctctat tccaaaaact agctacattc
    34261 ccatggaaaa cagcgcttgt atagccttgc ttttgtccaa ggatcgctgg tgcggcttga
    34321 aagacattgt ctgacccaag ttctgtgaat aatgagcctt gaggtaaacc aaatgtgcca
    34381 gtttccaaca tattttcagc atcacttgtc tttccctgtc ctacttcatg aaagaaattg
    34441 tcaaagctga tcgttgcttg attctggaaa atactattta aaaacggtgt gacttcttgt
    34501 ccgtctacct tcatattaat caaaaactgc tggaaacttt ctaaatggag aacgatcaca
    34561 tttttccctt tagcgatccc aaatgtttcg ggatttggct ctgcataatg tttctttgta
    34621 taatctaaaa cttcatcaat cccattactg cttgcatgcg cgcgaacact gctagtcatt
    34681 cctgttttga ttccgtcata aatagtatac gcatcgattc ctagatattt gacaatatat
    34741 gaacggtcaa atgttcgctg cagcaattgt ggacggttgg cttcgctcag cgctagattt
    34801 cctgaaaaaa taagccctga aagacaggta acagcgatag ccatcggttt gccgacaggg
    34861 ttgcttttga taggtatttt cttgaccttc aaccaaacca gtagcccaat gaacacagcg
    34921 atatccagcc agtagatgac atcatggggc ttcatcaaag aaaaggagct tcctgacaat
    34981 ccttgcgaca ctttcgaaaa gcctaataca gatttgactg taatgaaatc tgtgaattcc
    35041 cgataaaaaa taatattcaa gtacaacaaa agcgtattta aaatatcaat gatcaaaaga
    35101 atagctaacg ctggctttgg ctttttaata tacaaagaaa ggccaaaaaa caataaggta
    35161 gtcgcaattg ggttgatcca caagataaag tactggatcg taccttccac acctaatgaa
    35221 aaatctgcat agtaggcaat gatcgtcttc agccagaaaa agaaaacagc tgtcaaaacg
    35281 attgctaaac gattttggca caatttttta agttgtgtca cgtatatcac ttctctctat
    35341 tcacattttt tcttattttg ctcctgaaaa atgaaaattt cctaaaaaag ttgttatctt
    35401 aagatttttt tattctttct aaagattatt ccgtccactt ttcacagcat aaaaccgctg
    35461 cgatgtaagc gattaaacta atgaagagat aaaagtcttg tggtgaccgc ttcactccac
    35521 ggtattcaaa agccagtttc tcggtattcg tacaaaaaaa gaggctgtga caaaattctt
    35581 gtcgcagctc cttatttctt gatgcaggac agctttttca caacttcttc cttaaacggt
    35641 gttctcctgc caactcctcg gccacaagtc acaatattta taaaacatga gaagctgttt
    35701 tttgaaattg ttccttgtgg ctcagagtta aacggcaggc tcaagacctc ttgatacacg
    35761 gtgttcaatc agctgaccct cggtattaga ttatgtcagg acgctaattt tacaacctct
    35821 tggtaaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
    35881 aaggagctat tttttgaaat tgctccttgt ggctcagagt taaacggcag gctcaagacc
    35941 tcttgataca cggtgttcaa tcagctgacc ttcggtatta gct
//
LOCUS       DAMEET010000020        34903 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.020, whole genome shotgun sequence.
ACCESSION   DAMEET010000020 DAMEET010000000
VERSION     DAMEET010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 34903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.020"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(324..1172)
                     /locus_tag="PB780_000837"
     CDS             complement(324..1172)
                     /locus_tag="PB780_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDE0732908.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1396..3336)
                     /gene="thrS"
                     /locus_tag="PB780_000838"
     CDS             complement(1396..3336)
                     /gene="thrS"
                     /locus_tag="PB780_000838"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDE0732909.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3673..3852)
                     /locus_tag="PB780_000839"
     CDS             complement(3673..3852)
                     /locus_tag="PB780_000839"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HDE0732910.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3899..5722)
                     /gene="cadA"
                     /locus_tag="PB780_000840"
     CDS             complement(3899..5722)
                     /gene="cadA"
                     /locus_tag="PB780_000840"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDE0732911.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGVKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            6000..8279
                     /locus_tag="PB780_000841"
     CDS             6000..8279
                     /locus_tag="PB780_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDE0732912.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETEIDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8372..9019)
                     /locus_tag="PB780_000842"
     CDS             complement(8372..9019)
                     /locus_tag="PB780_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732913.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNNVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(9198..9917)
                     /locus_tag="PB780_000843"
     CDS             complement(9198..9917)
                     /locus_tag="PB780_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDE0732914.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(10048..11199)
                     /locus_tag="PB780_000844"
     CDS             complement(10048..11199)
                     /locus_tag="PB780_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDE0732915.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKTTGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTYSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDVGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11416..12156)
                     /locus_tag="PB780_000845"
     CDS             complement(11416..12156)
                     /locus_tag="PB780_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDE0732916.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEIFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(12160..12516)
                     /gene="rsfS"
                     /locus_tag="PB780_000846"
     CDS             complement(12160..12516)
                     /gene="rsfS"
                     /locus_tag="PB780_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDE0732917.1"
                     /translation="MIDSKEQLKIAVEAADSKRAEDIIALDIRNVSLLADYFMICSAN
                     SERQINAITEEIIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERSFYNLE
                     KLWSDAPLVDLSEWLD"
     gene            complement(12540..13133)
                     /gene="yqeK"
                     /locus_tag="PB780_000847"
     CDS             complement(12540..13133)
                     /gene="yqeK"
                     /locus_tag="PB780_000847"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HDE0732918.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(13117..13767)
                     /locus_tag="PB780_000848"
     CDS             complement(13117..13767)
                     /locus_tag="PB780_000848"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDE0732919.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13777..14088)
                     /gene="yhbY"
                     /locus_tag="PB780_000849"
     CDS             complement(13777..14088)
                     /gene="yhbY"
                     /locus_tag="PB780_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDE0732920.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(14104..15213)
                     /gene="yqeH"
                     /locus_tag="PB780_000850"
     CDS             complement(14104..15213)
                     /gene="yqeH"
                     /locus_tag="PB780_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HDE0732921.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(15210..15737)
                     /locus_tag="PB780_000851"
     CDS             complement(15210..15737)
                     /locus_tag="PB780_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HDE0732922.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15964..16749)
                     /locus_tag="PB780_000852"
     CDS             complement(15964..16749)
                     /locus_tag="PB780_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDE0732923.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRFDQHELLEKRYKRFRQY"
     gene            complement(16742..17611)
                     /locus_tag="PB780_000853"
     CDS             complement(16742..17611)
                     /locus_tag="PB780_000853"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDE0732924.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHML
                     LELHTQKGWIDRG"
     gene            complement(17612..18991)
                     /locus_tag="PB780_000854"
     CDS             complement(17612..18991)
                     /locus_tag="PB780_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDE0732925.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPTRHIEVQILGDHF
                     GNVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18999..19418)
                     /gene="fabZ"
                     /locus_tag="PB780_000855"
     CDS             complement(18999..19418)
                     /gene="fabZ"
                     /locus_tag="PB780_000855"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDE0732926.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19443..19919)
                     /gene="accB"
                     /locus_tag="PB780_000856"
     CDS             complement(19443..19919)
                     /gene="accB"
                     /locus_tag="PB780_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDE0732927.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPETMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19922..21157)
                     /gene="fabF"
                     /locus_tag="PB780_000857"
     CDS             complement(19922..21157)
                     /gene="fabF"
                     /locus_tag="PB780_000857"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDE0732928.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIIGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(21176..21913)
                     /gene="fabG"
                     /locus_tag="PB780_000858"
     CDS             complement(21176..21913)
                     /gene="fabG"
                     /locus_tag="PB780_000858"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDE0732929.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21925..22842)
                     /gene="fabD"
                     /locus_tag="PB780_000859"
     CDS             complement(21925..22842)
                     /gene="fabD"
                     /locus_tag="PB780_000859"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDE0732930.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22949..23173)
                     /locus_tag="PB780_000860"
     CDS             complement(22949..23173)
                     /locus_tag="PB780_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDE0732931.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(23226..24188)
                     /locus_tag="PB780_000861"
     CDS             complement(23226..24188)
                     /locus_tag="PB780_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDE0732932.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(24198..24638)
                     /locus_tag="PB780_000862"
     CDS             complement(24198..24638)
                     /locus_tag="PB780_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDE0732933.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24944..25909
                     /locus_tag="PB780_000863"
     CDS             24944..25909
                     /locus_tag="PB780_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDE0732934.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRAQLPDLQ
                     EMTFEQKKKELQGLFQGQNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKHFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMIASADVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQSEIELLNKKEAEKQLN"
     gene            complement(25966..26196)
                     /locus_tag="PB780_000864"
     CDS             complement(25966..26196)
                     /locus_tag="PB780_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HDE0732935.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(26282..27169)
                     /locus_tag="PB780_000865"
     CDS             complement(26282..27169)
                     /locus_tag="PB780_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDE0732936.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(27258..28481)
                     /locus_tag="PB780_000866"
     CDS             complement(27258..28481)
                     /locus_tag="PB780_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDE0732937.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28498..29544)
                     /locus_tag="PB780_000867"
     CDS             complement(28498..29544)
                     /locus_tag="PB780_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDE0732938.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYSIRVPIQAISNGIDVEKYRPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(29708..31435)
                     /gene="ptsP"
                     /locus_tag="PB780_000868"
     CDS             complement(29708..31435)
                     /gene="ptsP"
                     /locus_tag="PB780_000868"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDE0732939.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(31435..31701)
                     /locus_tag="PB780_000869"
     CDS             complement(31435..31701)
                     /locus_tag="PB780_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HDE0732940.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            32180..34414
                     /locus_tag="PB780_000870"
     CDS             32180..34414
                     /locus_tag="PB780_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDE0732941.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34562..34882
                     /locus_tag="PB780_000871"
     CDS             34562..34882
                     /locus_tag="PB780_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HDE0732942.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT         9882 a         7532 c         6060 g        11429 t
ORIGIN      
        1 taatattttt ttcataaaat ctttcatcat ttcctatcct tccgcctatt tacattcatg
       61 cttcattgta cccaaaaact tcttaataaa aaagaaaaaa tgggcggagc aagaaattct
      121 ttacctaatt tcaagataaa atcaactttt tgtttcacag caatcactta aagttacata
      181 tccaactatt tttttcatta aaaaataaac ttatttttga aaattatgtt ttcttaagtc
      241 gtgaaacact ttgatggatc atttatttgt ggaaaactac gttttgttat tctaaaaaac
      301 ttatataaaa aatagtaagg tttttatttc gaatccataa aatattcttg gattgcttct
      361 cgtgtttttt tcactttttc gctaagcttt tcgtttttag ccatatagaa ataaaaaatg
      421 atatcgatcg tagcaaattg ggccaaaagt gaattagttg ccgcacttcg attcgtgatt
      481 tcttttggtc tagatgtttg tacgagtaca tcggctatct tgctaagcgg gttatttcca
      541 aattgcgtca acgcaatgat ttcgatattc atgcttttcg ccagctcggc taatgcgact
      601 acatctgcac ttctacctga gtttgaaaca agccaaagca ctttcttttt ctcattgctg
      661 acaagttgcg ggagcaagac gtggatatct ttttcaaaaa tgattggctt accgacacga
      721 gaccattttt gcagaatatc ttctactact aaagaagagg cgccaacccc atataaaaaa
      781 acagtctctg ccgattccaa acactctacg actttttgga tcgtttcttc ttctagaata
      841 tctactgttt cttttatcgc tacttgtgca ttgttaccaa gtttgttttt gatagagtaa
      901 aaggactcat ttgcttctat atcactaaaa ccttcatcga ttccagtctg tagttctgtt
      961 gaaatcgtga ttttgaagtc cgtcagactt tgaaaaccga tttttttgga aaaacgtaca
     1021 actgtcggcg cactcaaccc ggaagcttct gctaattcat tagctgtcat tgtagggacc
     1081 ttctcaatat taccgataat gtagttggcc aacttttttt ctgctgaaga ataatcttcc
     1141 atcatacttt caatacgact aattacgctc ataagttcct ccaccctttt aatgatagtc
     1201 tatgtaagta taccatatca gacccttatt cacggtcgta tactggataa aaaacaaaaa
     1261 aacaatccgc ccaacagaat atcttatccc atcaatccca cagattctat tgagcatctg
     1321 tgtctttgag gaatacctgt tatctattgt tcggattgct ttctatctac tactttttta
     1381 caggaaattt acctattagt tttctctgct gtaatttttg acttctgctt ccatagcatc
     1441 aataaagata ttcaaatctt ctacagctgt ttctttactt ccataacgac gtacgttcac
     1501 tgttgcatct tccaattcct tgtctccaac aactaattgg taagggatct tttgtgtttg
     1561 agaggcacgt atcttatagc ccattttttc attacgatca tcaacttcta cacgcaaccc
     1621 cttcatttgc aaacgttctt tgatttcata agcataatcg ccatgtgcat caacagaaac
     1681 agggatgatt gttgcttgta taggtgccaa ccatgttgga aaagcgcctt tgtatacttc
     1741 tgttaaataa gcgacaaaac gttccattgt agaaacaatt ccgcggtgga taactactgg
     1801 acgatgcgta ttttccccgt cttctccaac atatgtcaaa tcaaagcgct ctggcaataa
     1861 gaagtctaat tgtatagttg ataatgtttc ttctgtacca agagctgttt tgacttgaac
     1921 atctagtttt ggtccgtaaa aggctgcctc gccttctgct tcaaagtaat ctagttctaa
     1981 ttcatccata gcggctttca gcattgcttg ggctttttcc cacattgcgt catcatcaaa
     2041 atatttatct gtattgtttg gatcgcgata gctcaagcgg aaacgataat ctgtaatatt
     2101 gaagtcagca tatactgcca ccatcaattc aagcgtacgt ttaaattcat ctttgatttg
     2161 atcaggacga acaaatgtat gaccatcatt caacgtcatt tctcgtacac gttgtaaacc
     2221 tgacaaagca cctgattttt catagcgatg catcattcct aattcagcga tacgaattgg
     2281 taattcacga taagaatgga tcgtgttttt gtagaccatc atgtgatgag ggcagttcat
     2341 tggacgaaga acaagcattt cgccatctcc catatccatt ggcgggaaca tgtcttcttg
     2401 ataatgatcc cagtgtccag atgttttgta caactcaaca tctcccatta ttggtgtata
     2461 tacatgttgg taacctagac taacttcttt gtctacgata taacgttcga tcgtgcgtcg
     2521 gattgttgcc ccttttggaa gccagaatgg caaaccagaa cctacttctg gggaaaccat
     2581 gaacaagtca agttcttttc ctagtttacg atggtctctt tcttttgctt cttcacggag
     2641 acggatatat tctttcaaag ctttcttgtc aaaaaaggct gtcccgtata cacgttgcat
     2701 catttgattg tttgagtttc cacgccaata agcacctgcc actgataata atttaaatac
     2761 ttgtatacgg ccagtggatg gaacatgtgg tccacgacac aaatcgatga attcgccttg
     2821 ttgataagca gtaatcactt cttcttctgg caattcacta atcagttcta ctttgtacgg
     2881 atcacttgca aaaatttcta atgcttcctg ttttgaaaga acacggcgtt cgattggcag
     2941 attttctttg acgattttca tcatttctgc ttcgattgcc ggcaaatctt ctgcagttac
     3001 ttggttttgt ccattatctg tatcgtagta aaaccctgaa tcgatagctg gtccaacgcc
     3061 aaagtggata tttggataga ggcgacgcat ggcttgggcc atcaaatgag ctgctgaatg
     3121 acggactaag ccaagtgcat cttcatggtc tggtgtgatg atctcaatac ttgcatcttc
     3181 ctcgattgga cgagtcaaat caacgagttt tccgttcact ttaccagcta aagctttttt
     3241 tgctaagctt tttgaaatgc tttcagcaat tgccaatgtt gttgttccag actcaaattc
     3301 tttgactgca ccatctggaa aagtaatttt tatcataata gtttcctcct tgaaatgttt
     3361 tgattgatca gatcatcaag ctgaaaaaca aaaaaatcct agcatctctt gaaaagagat
     3421 actaggacga aaagttcgtg gttccaccta atgttcaaac gcaaatgcgt tcctcaagcg
     3481 ttgtaacggt ccgaccgccc ttgcttcaac aagggtttcg aaagtggtca tcctttggtt
     3541 ttctcctgga aaagtttcag cctagctttt cctctcttat cgaagactct caaattcagt
     3601 tggctttctc atcaatcaat tatgtgttta tttaaccact aataaataga aaaatcaagt
     3661 gggaaactaa acttattttt tccattcccc atttacaccg tatgttcctt ttttcatctc
     3721 attgatgatg acatggatgt gttcttttgg tgcctgcgtg tttttatgaa cagcttccgt
     3781 gatatctttc atcatatttt ctaattgttc cggactgcga ccttctacta attcgacatg
     3841 tacgaatggc ataatgctct ctcctctttc tgatcgttct tttttcaact cgattctatc
     3901 atactatgtt tgtcttgggt actgaatcag gaaaaatggc tggatctttt tttgctctca
     3961 aggcactcaa aatagagacc gtatccacga cttcttgcaa aacagctccg aataatgcgg
     4021 ggattagtcc cgtacttgca attaacatca aaaaaataca aataaagatg ccaatcagta
     4081 cagactgttt agctattctc atcgtatctc ttgaaatcac tactgctttt gctacacgca
     4141 agagatcatc tttcaagatg accgcatctg cactttcact agcagctgta gaaccatgag
     4201 cccccattgc aataccgata tctgcagcag caagtgctgg agcgtcattc acgccgtctc
     4261 ctaccatcgc aacaggtctt tgatctttag ggatactttc taaaatggtc agtttatctt
     4321 gaggcaagct acgggcatgt acagaatcta attggacttt ttctgcaatc gtttgagcaa
     4381 tggcagatcc atccccggtg atcatcatca aatttttcac atcaagtttt cttagctcct
     4441 tgattgtctt tgctgcttct gggcgaatct gatccgaaaa taggatcgtt cctgcaaaag
     4501 atcgatcaat tgttacatag attgctgtat tggacgtttc tatttcttca gctgaaacaa
     4561 aagaggcacg tccaatcttt acttcatgat tattgactac tgcttggatt ccttgaccgg
     4621 tcacttcttt cagttgacta acaggataca gttggctgtt tttcacgttg ttcactaacg
     4681 aacgagctaa gatatggcta gattcctgtt ctgcactagc tgcatacaga agtagctctt
     4741 ctttttttac tccattgact ggctgaatcg cctctacttc caatatccct ttggtaagcg
     4801 ttcctgtttt atcaaaagca atcgtttttg tacgtgcgag cttttcaatc gttgttccag
     4861 tttttacaac gatcccgttt ttacttgaac ggctcattcc tgcaactaaa gcaatgggcg
     4921 cagctagaat cagtggacaa ggtgatgcca ctaccagtac ctcagcaaat cgtaaagggt
     4981 cttttgagaa ccaccaagaa ataccaccga tcaaataagc aatcaacgtg aaagggactg
     5041 cataacgatc agctaaacgg acaaatcggg caggcttttc ttttgattct tcgactagtt
     5101 taataatcat ttgatactgg ctgtctgcgg cacgtttttc agcgatcatt tggatcgatc
     5161 cgtctccatt taaagaccct gacataatct gatctccagg tttcttggtt tccgggcggg
     5221 actcacctgt caaagaagat tcatcgaccg aagattctcc tttggtcact cgaccatctg
     5281 cgggtacaag ttcgccaggc ttgactagta actgatcatc aatatttatt tcatcgatta
     5341 ctatatcctg caattttcct tctttgaatc gatgggcaat tcttggggtg ttatctaaca
     5401 aagactgcag ttctttaccc gctcgtttat tcgcgtaatc ctctagacta tctccgcccg
     5461 taagcatgat caatatcatc aaactagccc agtattcttt gacggctaat gttgcaagga
     5521 ttgcggtaat cgctaataca tctacgccat atcttcctga acgtaacgtc ttgatcattt
     5581 ccaaaaacat caagaaggcg agaatccctc cagtaaatgc gacaatcgca aatgcaaggg
     5641 ccggctggtg aaaaacaaat tcacttaata aagctatccc accaataagc aaagtaagta
     5701 aaaatttttt gaaatgtgtc attgtttttt ctcctctttt atgtaactta gagaggttag
     5761 taataaattt ccagctgcct ctttcatctt aagcatagtc cttgactagc actttttaaa
     5821 acaataggcc ttaattgaaa acaactatca ctatcagcgt ttccaaaatt catcagttct
     5881 acaaacaaaa atattcagca aggattactt tttcgattca tcagttaaaa agtacctttt
     5941 ttcgaaaaac tatgctatac ttttaattcg ttatttacgg cggaaggagc aaacatgcga
     6001 tgaatgagaa acaactagaa caaacgcacg tggatgaaac gatcgaacta atccagatcg
     6061 aacaaaatat gctggataca aaacaacaag aattgactca ggaaatgaat cattcgacaa
     6121 aagacacagc taatcacaag attcgagctg gttccaatga agctttttat gaatctgctg
     6181 ttgaatatcg ccaacatgag caggaattgc ttttgaaata ccatactctt gaagcacagc
     6241 aaaaacgcct gcaaacgttg tctgtcatga aagatagtcc ttattttgcc cgtatcgact
     6301 ttaaagaaga ccgtgaaaca gaaacattat atctaggtat cgcttcttta agagatctgg
     6361 cagaagaaac aatcgttatt gactggcgtg caccaattgc caatctttat tatgaaggtg
     6421 agattggtcc cgcattttat gaaactgaaa tagatagaat agatgtcgat ctgcagttga
     6481 aacgacaatt taaaatccaa gaaggtcgta ttttatctat ggtagatacg tcggaagtaa
     6541 tcaatgatga ctttttgcta gagatattgg atgaatcatc aagcaaccat atgaaaaaca
     6601 tcgtctctac gatacaaaaa gcacaaaatg caatcattcg ggacacaaca agtaaagtaa
     6661 tgctgattga aggaatcgcc ggaagtggaa agacatccgc tttgcttcaa cgtattgctt
     6721 ttctgttgta tcataatcgc aagtggctcg aggctgaaaa cgttcttcta ttttcaccca
     6781 accatttgtt ttctgattac atctcgactg tattgccttc attaggtgaa agcggcgtgc
     6841 ctacacagac attcaaaaat tatttggtgc agctgctgcc agagttttct ttactagatg
     6901 aacagcaaca agaaagtggt tttttgctag gggaaaaaga tccgatacaa gtgatgaaaa
     6961 gcggattgac cttagtcgat cagattgatc gctatatcca aggcatcaca aactacggtc
     7021 ccttgttccg tgatatgaaa ataaatggcc gtacgatcct atcaaaggaa tcgattcgcc
     7081 aatggtataa agaaacaaat gaacaattgc ctttacatca tagactctct ctattacaaa
     7141 cgaagctcct gaaaaaatta ggcggtctgc aaaaagacga aacacgtcag caatgggtca
     7201 aagatctggc ggaagaacag ctccaagaac tatacgcaac agatccaaat cttgaatata
     7261 cagaacaaaa ggagcgttca ttacgtaaaa aactggccaa ccaaatcgta cgtaaaagat
     7321 tccgtcgaat ccatcgaggg atcatgaatt atcaatttgt caatataacg aaacaatatc
     7381 tccactttct acaaactgtg ccactttctc ttctaacacg acataaaatc agcgagacca
     7441 gatggcagga acatttgaca actgttcgta cacacttgcg tcaaagagaa ttgcgtcaag
     7501 aggatgctgt actattcttt ttactgatgc gccgaacata tccagtgacg gttacacaca
     7561 aagctcgcta tatctttatt gatgaaatgc aggatttcgc accatcacaa gtggcattgc
     7621 tgcaagcctt gttcccaaaa gcaaatttga ctttctgtgg agatctaaat caaaatgtct
     7681 ttggaaatga aacgatcgtt ggatcattgg aagacctttt cgtggaccgt gaagtcactc
     7741 gtttccaatt aacgacaagc tatcgctcca caaaagaaat cactgatttt gctaatcatt
     7801 ttattgtgaa agaacatcaa gtagaaacaa cagcaagaaa aggagacctt cctttgctca
     7861 tccaaggaac aacagatgat atcaaattga attggttagc agataccatt cgaacgactc
     7921 agggaaaagc ccggtattgg cgtacagcaa tcatagctaa aacacaagaa gaatgcgaag
     7981 ctctttatgc tcaactgcca gacgagttga aacaacaagt ccagctgatt ttaaatgaga
     8041 gtgactttat gaaacgttcc attattttac ttccagcctt tttagcaaaa ggtctggagt
     8101 ttgaccgagt tttcttgtgg gatgtagatg cagaacattt ttcttctcag caagacaaat
     8161 tgatccttta caccatgtgt acacgtgcga tgcatgaatt gacccttttt acctccaata
     8221 atgaaagtcc tttgttaaac aatatggata tttcatctta tgagaaaaaa gtactgtaag
     8281 taaaaagaca actgctatca ttaatgttag cagttgtctt tttatttaca ataataattg
     8341 cagaaaaagt aattcaatca tttaaggtca tttattctat atataaatcc aaatctgttt
     8401 caggatattt taatttgaca agcgaaataa tcattttcag ttcttccatg ttttctggat
     8461 attctgggtc aaataccttg ctatgacggc caatcccgcc attgtcgatc aatcggtaaa
     8521 ttcgattcca ttcaatgaat tggtcaattg attggaaatt gctgattgga tagccgccac
     8581 aatccaaaat ctctgataca cgaaatgcta ggtatgcact atcgatcaca ttattttttt
     8641 tcgtgtctat cttctcattt ctttcgataa gaattttttc aggacgatgc ttcaagctcc
     8701 actctaatag tagaaagaaa gcctccatcc gatactcttc ttcaaataac cgaaaggctg
     8761 aatcagaacg acgaaaaagc agcggaatca gataattttg tctataaatc agcattggtg
     8821 cgcggtccac catacactct aaataacggt tcagataccc tgtaccgaca tagtttccta
     8881 atcgatagcc ttcaacgccg aatcttgcgt atttttgcgc taattttgaa caatcagtta
     8941 cgttcacagc taactcttct actttgcggt aaccgcttaa gatacgttca aaaggttcta
     9001 aagtgatttt tgcttccata ttcttccccg tccatcttcc acatacttca tatcatttca
     9061 catgatactt atgctctcat cataccacag gttgaattag aaaagaaaat aaatcacaca
     9121 aaaaagcaag acaatctgtt agtgagattg tcttgctttt cgaaaaagac tgaatacttt
     9181 tcgttaaaac tggttgctta gtcttctaat gccacattat ggaacacatg ctgtacatct
     9241 tctagatctt ccagtgcatc aatcattttt tcaaatgttt cttgatcttc tggagatagt
     9301 gttacctcgt tttgagggat catttggatc tcagcgattg aaaaatcagt gattcctttt
     9361 tcttttaatg cttcttgtat actatggaaa tccgctactt caccgtaaac gatgatttgt
     9421 ccttcttctt ccattacatc gcgaacatcg atgtcttttt ccattaggta ttcaaggatc
     9481 tcgtcagcgt cttctcctgc aaaagcaaag attgctgtat tgtcaaacat gtaagaaacc
     9541 gctccggaaa cacccatgtt cccgccattt ttcccaaatg ctgcccgtac gtctgctgct
     9601 gttcgattca cattgttcgt caatgtatca acaatcacca ttgagccgct tggtccaaat
     9661 ccttcatagc gcaattcaga gtatgtttca tctcctgcac cttttgcttt ttcgattgct
     9721 cggtcaataa tatgttttgg tacattataa gttttagcgc gatcaatgac gaaacgtagt
     9781 ttttggtttg catgcggatc aggatcacct gattttgctg ccgcgtagat ttcaatccca
     9841 aattttgcat aaatccggct attattagca tctttagcag ttttctttgc tacgatattt
     9901 gcccatttac gtcccatatt ttcacttcgc tttctgattt gatagtttca taacgcaaaa
     9961 acgtcctgtt ccattatact tgtgaatcac gattctgtga agcttttttt ctttcttcct
    10021 tcattcttct catttttagt caattatcta tactcggatt gggatttgcc cgaaattctg
    10081 ttccggaata ttgcctccca attgatagat ttgatcactt ctaactaaca gttccgcttc
    10141 ttgtgcctct ttttttccga ttcgggaata aatagaaatc tctttttttt cttttaagaa
    10201 tcttcttcca acatcggtaa atccaagtag tcgaagataa tttttatttt gttggacagg
    10261 aatctcttgc tttttgatat tcagcaagac ataacaagat aatcgttgga tccgtgtcca
    10321 tgtatatctt tttgttttta cctttgacac aaaatcctca aaactatcag cagttgcagc
    10381 tgctgttttc atgcgcgatt ctattccttc ggtcatctga tagatatctg tcagttccag
    10441 caaagtagaa gataacaacc gataacgcaa atacggccaa taagattccc aagtctgcag
    10501 cgaataagtt gaaagatctt cttctgtctg aggcggcaca agtccagcta ttctttttcc
    10561 ttcttctaac gcatggcgaa tggctgttgc actggctatc ctctgatctg tgatgatttt
    10621 ttcatgatag cctgcacttt tacgtcggat cggatgtaat tccatagggt ggacatactt
    10681 ggcattttcc tttgcataac ttaatcccaa gatatgattt ggcgaagaaa aatcaagcgt
    10741 aatatcggga tacagactgc gaaaaacctc agtcattttt tgcggataac tatacgtttg
    10801 atcagacagt tcttgaaatg cctcttctat cctatcttga ttctcttgga caaagcgtcc
    10861 gaaactgctg taatcaaacg aagtctcttc ttctgttcca aaacacaaac tgtcacagtc
    10921 cagtgtttgc aagattttta ctgcaccttt agcaaaatag tcagctgact gaaccgccca
    10981 ttcgacggga agctccacta ctaaatccac accattttgt aaagcttcat tcgcgcgatg
    11041 ccatttatcg atgatagccg gttcgcctcg ttgcaaaaaa ttcccgctca ttacagcaat
    11101 gacgacctct gctcctgttg tttttcgtgc catttccaca tgatagcgat gtccattgtg
    11161 aaaaggattg tactctacga taatcccgca gcttttcatg cgcatcctcc ttcatagtta
    11221 tcgataaaaa agaaatagag acaagtaaaa aaaggcaaaa atcggaaaaa atacccgaac
    11281 tatataaact attctcgcaa acacaatact tctgcgacga aaagctgcac tgcaagagca
    11341 atgcctatat cgttcggata tataatttat gttgaagatt tattcttttc ttgttaactc
    11401 gagcaggcaa aaccgctagt ctttgtatgc tacaaagaac cagcgctttg atttctctga
    11461 tgctgcttcg tctgtaaaat cagaaaatgc ttcgactttc gaaaagccgc tgttttccag
    11521 cattcttaaa taactttcca atggataggt acgctcttga tgtagttcat cttcacgaat
    11581 aaatcgacca gacccttctt gttcttcaac gaaaaaggta agaaaatgtt cgatgctgtg
    11641 tttctcgctt cctggataac tatcccacaa aaaagcaaaa ttatcccctt gataatgata
    11701 gctatattct ggaaaaattt catctacttg ataggtggag tgtacgtcaa ataaaaatcg
    11761 gccattttct tctaacgctt gatagacttc atcaaacact tgctgtacct cttgtctatt
    11821 tttcatatag cacagcgaat cagaaaaaca ggtaattgcc tgatattgtc cgatttctga
    11881 taaatcgagc atattgcctt ccacaaattg gatcgatgcg tcttcttcat atgctcgctg
    11941 gctggcaatc atcaacatct cttctgaaag gtctaaccct gtcactttat atccatcttt
    12001 tgcaaaggca actgctaatg ctccagtacc gcatgccagt tccaaaatat tttttgtgtc
    12061 ttcttgtaaa tgacgtttcg aaaaagctag ccattgctga taaagtgttt cgtccatcac
    12121 ttcatcgtaa atgaaagcaa atgtttcata agccatatgt taatccaacc attcactaag
    12181 atcgactagc ggagcatctg accatagttt ttccagatta tagaaacttc gttctggtgc
    12241 atggaaaaca tgtacgatca catctccaag atcgatcaat acccattttc cgccttcttt
    12301 tccttcaata cgttttactt catatttgtt ttcttcttct ttatcaatga tttcttctgt
    12361 aattgcgttg atttgacgtt cgctatttgc tgaacagatc atgaaataat ctgccaacaa
    12421 ggaaacattt cgaatgtcta aagcgataat gtcttctgcc cgttttgaat ctgctgcttc
    12481 aaccgcaatt tttaattgtt ctttactatc tatgatgatt tcctcctaat atgagcagct
    12541 tatttttttg ctacccagta attatatgtt tcgattgttt ttggataaat ctgttgttct
    12601 tgcgcaagca aatgggccaa tgtatgtttt gtttcaaatg ctacagcttc gtctagatca
    12661 gcccatgcaa tggctcgtgc atcttgtacc ccaggaaaat ctcgacctgg ttcaatatag
    12721 tctgctacat aaatgatttt gtcaaggaga ctcatcttag cagccccagt cgtatgcacc
    12781 cggattgcat gaagaatttc tgcatcggtg attcctaatt cccgctctac aaacgaagcg
    12841 ccaacaagac catgccagat tgcattatta taattcaata actctggatc aaaaccgtct
    12901 cttacgatta ccattttgaa ctcctcatct ggtcgttctt ttgcataatc atgtgtcaat
    12961 gcagcaatac tagctttttc aggagaagca ccgtattttt tggcaagagc cacggctgtt
    13021 tcttctactc caagcacatg cttgaagcgt tgttcgctca ttctcatctg tattttttgc
    13081 atcaggtttt ctctagaata agcagtatat tcattactat agtccatatt catacaaccc
    13141 ttcattttga atataatcaa ttactttatc tggaacaagg tagcggatag aacagccttc
    13201 tttgatcttt tgacgtattt tcgtggaact gatgtcgatc tcaggtacat ctacccaaat
    13261 tacaggatat ggagtatccg ttgtgtatcc tggtcgacgt atacccacaa agttgaccat
    13321 agaagtcagc tcatcgattt tataccattt tggcaaatat tctaccatat ccccaccgat
    13381 aataaagtaa taatctgtat ccggattgtt ttgcgtcaat tccttcatag tatcataggt
    13441 gtaacttttc ccaccacgag ctaattcaat cgtttcaatc tgcaaaaaag gattgtcttc
    13501 cacagctaat tccaacatgt tgagtcgatg ttttgcatca atcgtctgtt tctcatcaac
    13561 atgaggtggc tgataagaag gcatcaaaaa tacgcggtca agacctaaat tccggccagc
    13621 ttgttcagcc atcactaaat gtgcaaggtg aacgggattg aatgttccgc ccaagatccc
    13681 tacttgcttt ttcttctcta agaattgagg catttcttca ggaaataatt gtgaacgaac
    13741 aaatgtctta gcttgcgtat tcatcatggt cactccttat attgctttta cttcttttga
    13801 tagcctttga tacttttctt ttgtggaagg tttgtataat acaataacac gtccaatgat
    13861 ttggacgatt tcgcaatcta tttctttttc taatacttga gctgcttctt ctgcaatctc
    13921 atctgtattt tgaagtaacg atactttgat cagttctctt ttttctaaag cttcttcaat
    13981 ttgaacgatc attgctgaat tgatcccacc ttttccgatt tgaaaaatcg gctgaagatg
    14041 atgcgctttg ctacgtaaaa aacgcttttg ttttcctctt aaactcataa ttttccttct
    14101 tccttaaatc aatgctttgc gtttcaatac atctacaccc tttggcgccc agcccgcaat
    14161 tactactggc gctgttacag tgatccagcc tagaccggca aaaacgatgt ctgttttttc
    14221 tttcacagaa aactcaaaac gaacaagctc cggaaatgcc tcgacttcat caggacgagg
    14281 aggctgtaat aatccaccta catgtttttg ataaaattcg tctgcttttt ccagtttcgt
    14341 tcgatgaata tttacatcat ttgacacata agcaataaat gaagcacgat tgccgctgat
    14401 aaaatcaaat cttgcaagtc cgcccaagaa aagtgtttgc ccttcattca gctgataaac
    14461 tttaggtttg atttctttta acggtgcaat gatccgcaaa tcttttttac ctaagtagtg
    14521 cgccatttga tggcgatgga taattcccgg tgtatcaatc aaaaaatgcc cgtcatctaa
    14581 aggaatctcg attttatcca aggtcgttcc tggaaactgc gaagttgtga ttacatcttg
    14641 tactcccgct gtatttttga tgatttgatt gataagcgtc gattttccga catttgttac
    14701 tccgacgata tagacatccc gtccatcgcg gtattcttcg attttttcta atagttccgt
    14761 catctcatga gatttcttag cactagttaa taatacatct actggacgaa ttcctgcttc
    14821 gtaagcacgt tccttcatcc attgtgttaa tttagttttt ttcaatgatt taggtaatat
    14881 atccactttg ttcccaacta aaagtactgg attgtcaccg acaaaacggt gtagtcctgg
    14941 gattagtgaa ccattgaagt caaaaatatc caccacattg acgatcaatg catctttctg
    15001 ccctaattca ttcagtaagc gcaaaaagtc gtcatccgtt aatgagacat cttggatctc
    15061 attataatgg cgtaatctga aacaacgctg acaatagact tccccggaag ctaaaccttt
    15121 ttccaaagcc gtttgcggcg tgtatcctaa ttcaccgggt ttgtttgttt gtataacggc
    15181 accacagccg atacattgta attcttctgt cattcgattc cacctcgcca gcccatatca
    15241 gggtgttttg ctaatagata ggccataatt cttttttccc aaaaacgatt aaattgtgtc
    15301 ttccaactat ctgttgcgat aatgggtttt acaaggatgc tacgtatccc tgcacgattg
    15361 gctccacgta tatcagtcat aatctgatcg cctaccatta ctatctcatc atctgctaaa
    15421 tccaattgtt tctttgctat gttgatccct cttgctaaag gtttcattgc tcgtgcaaca
    15481 tacaaaagat caaatttttc aatcgctcgc gcgacacgtt ttgaattatt gtttgaaaca
    15541 acaacaacag ggattcctgc atttttcatt tctaaaatcc aagtaagcaa ctcttctgtt
    15601 ccatctggat tattccatgc aatcaacgta ttgtctaaat cggtcaatac tgctttgatg
    15661 cctagattct ttaattgggc aggtgtaatc tgatagatgg cttctaccat ccatgttggt
    15721 ttgtaatttg aaaacatgct gtctcctcat ctgcaaaaaa tgaaggagca cataaagtac
    15781 tcctcaaccc cgtattatac tgcatatcag caaaaaaaac tacctctata ctcgttcgtc
    15841 tttcagaaaa agcatttctt tacaaaatga ccaaaaataa agattgatca cagaaaaatt
    15901 tagaaacaaa aaacaactaa gcgagcaaga ccattcttta ctctttgctt agttgttaaa
    15961 cacctaatat tggcggaacc tcttgtatcg tttttccagt aattcatgct ggtcgaatcg
    16021 aacaagttcc gtcaatcttt caatcaacgc ttttttcata atgtgtgcga ctttatatcg
    16081 tggcaactgc tcgccgttga acgtttcagg aataactcga tcaacgattc ctaactcgcg
    16141 tagttctggc gcagtaattt tcatcaactc agctgcttct gcggcgcgac taccgtcttt
    16201 ccacaaaatc gacgcaaatc cttctggaga aagcacagca taaatcgttt cttccagcat
    16261 ccagacttca tccgctaaac ctagagcaag ggcaccgcca cttccacctt caccaatgat
    16321 gatggcgatg atcggtacct ttaaatcaga catttcgagc agatttctgg caatagcctc
    16381 tccttcgccg cgctcttctg ctcctacacc gcaatatgca cctgctgtat tgatcaatgt
    16441 aatcaccggt ctattgaact tttccgcttg tttcatcagt cgtaaagctt ttcgatagcc
    16501 ttcaggatgg ggagaaccaa aatttctgcg gatattttcc tgcagatccc gtcctttttg
    16561 aataccgatc actgttaccg gtttgccatc taatcgagcg attccaccaa caatcgcttc
    16621 gtcatctgca aacaaacgat caccatggca ttcctcgaaa gaatcgaaga tctcttcaaa
    16681 aaaatccaag cttgtccatc gttctttagc tcttgctaac tgaacgacat catgtgctgt
    16741 cttatccacg atctatccat cctttctgtg tatgcaattc aagaagcata tggattttct
    16801 gcttcaattc catgcgggga acaatctgat cgacaaaacc atgagataat aagaattcag
    16861 ccttttggaa atcttctgga agttcctgct tgatcgtctg ttcgatgacg cgtcttccag
    16921 caaaaccgat caagctttgg ggttctgcta gaatgatatc gccttccatt gcaaaactgg
    16981 cagttactcc tccggtcgtc gggtccgtta aaacagttag ataaaaaaga ccttcattgc
    17041 tatgccgttt gacagcagca gagatttttg ccatctgcat tagagaaaag attccttctt
    17101 gcatccgtgc gccacctgaa gctgtgaaca agacgactgg aagactttcc tttgttgctc
    17161 gttcgaataa tctggtgatc ttttctccga caatcgttcc catacttccc atgataaaat
    17221 taggatccat cactccaata atgaagggaa tcccttgaat ggttgcttta ccagtgagga
    17281 ctgcttcatg aagtcctgtt ttatcttgca tttttttaat tttttccgta tattcgggaa
    17341 atgctagcgg atctttggtt acaagatccg tatcccattc ttcaaaactt ttttcatcaa
    17401 taatcaaagc tagacgctgc catgcaccga ttcgaaaagc gtatccgcaa tgagggcata
    17461 ctttttcttc accgatgtct ttcgtataaa ggatatgttt gcaatttgga cactttgccc
    17521 acatgttatc aggaacagat ggcgagttag caggtttgct ctctctatta ggattgatcc
    17581 gaatatattt ctttttctta aacagagcca tctatgcgcc tcctatctcc gtttctggtg
    17641 tccaattcgg taaaaattct tcttgtaaaa atgctgtgct gtaatcacct gctatgactg
    17701 cagggtgact aatcaaatcc atctgaaatt ctgcgttcgt gatgattcct tctgtgataa
    17761 attcacttaa ggctcgctgc atcttcatta atgcttcaaa acgcgtattt ccatggacga
    17821 ttatttttgc aatcattgaa tcataatatg gcgggatcgt atatcccggg taaactgcac
    17881 tgtctacccg cagtcccatc ccacctgctg gaagcagtaa gttttggatc ttccctggag
    17941 aaggagcaaa atggaaagca ggattttctg cattaatccg gcattcgatt gcgtgtcctt
    18001 ggaatactac ttcttcttga cacacattca aaggctcacc ggctgctatc tccacctgtt
    18061 tttttactaa atcgattcct gtgaccattt ctgttactgg atgttccact tggattcgag
    18121 tattcatttc catgaaataa aaatcgcctt cttcatccat cagaaactcg atcgttccgg
    18181 cattttcata attgactgct ttagctgctc gaacagcagc gtctcctagt gcttctcttt
    18241 ttgtttgtga gatcactaca gaaggagatt cttccagtac tttttgatta ttacgttgca
    18301 aagaacaatc tcgttctcct aaatgaatga cattgccaaa atgatcacct aaaatctgaa
    18361 cttctatatg gcgagtagga taaatgattt tttccaaata catatcatca tttccaaatg
    18421 ccgctgctgc ttcttgctga gctgaagaaa aatgctgtgg gagctcttct tttgattgaa
    18481 ctttccgaat gcctttcccg ccaccacctg cagctgcttt cagcatcact ggataaccaa
    18541 ttctgtctgc tatttccaat gcttcttcta ctgtagataa tacaccagta cttcctggaa
    18601 tgactgggac atttgcttct tgcatcaatt gacgcgcatt gatcttattt cccattgcat
    18661 cgatcgttgc acttttagga ccaataaatg tgatattgca ctcttcacac atggaggcaa
    18721 atcggctatt ttcagataaa aaaccaaatc ctggatgaat tgcttcggct tttgtcacga
    18781 ttgcagcgct cagtacttcc tgaacattca gatatgaatc aacggctttt gccgggccaa
    18841 tgcagattgc ttcatctgct aactgtgtat gaagtgcttc ggcatctgct tctgaatata
    18901 tcgctacggt ttggatccct agttcgcggc atgcacgaat gatacgtaca gcgatttcac
    18961 cgcggttagc tatcaatact ttagaaaaca tatttcacct atccaatcat aaaggttaat
    19021 tccgcttcgg ctactttttt accgtctact ttagcgattc ctttgcctat tccggctgaa
    19081 gctttcactt tcaatatctc aacttcaagg tataaggtat ctcccggtgt aactttctta
    19141 cggaattttg ctttgtccaa tccgccgaaa taagccgtct ttcccttgaa ttgttcaaga
    19201 gaaagtaatg caactgctcc tgcttgagcc atcgcttcaa caattaatac ccctggcata
    19261 acaggttctt ctggaaaatg tccttgaaaa aagggctcat tgatcgttac atttttctta
    19321 gcaacgatcc gttctccttc aaccatttct tccactcgat caatcaaaag cattggataa
    19381 cgatgtggaa tgatttcttt tatttcttgt atgttcattg atttccctct ttcctgcttt
    19441 gcttattttt gctgaatcct aaagagtggc tgtccatatt cgacaactga ctcgttttct
    19501 acaaggattt ctgtgatcac tccgtcggcc tcactagtta tctcattcat cagtttcatc
    19561 gcttcaataa tgcagataac ttctcctttg gcaacttgat cacctacttt tttgaaagct
    19621 ggtttgtcgg gcgccggttg cagataaacg attcccacga ttggcgaaac aacctcgatt
    19681 ccatcaaata ctgccgattc tttttctttc attgtttctg gagcagttgt tgcttgttca
    19741 ggtatgacat tgactggctc cgaaactggc aacgtttcgt ttgtttgaac catattcatg
    19801 cctcgacttg ttttattttt attcatatac aattcaaatt gaccttcacg caaatcgaat
    19861 tccgttagcg aggattgatc aaattgactg actaattctc tgatttcatt gatatccaaa
    19921 attattcctc ccagcgtttc atgcacagaa ccgcattatg tccgccaaac cctaatgaat
    19981 tactcattgc ataagtaaaa cttgcaggtt tgctttcgtt tttgacaact gtcaagtcaa
    20041 tctcaggatc ttgtttttct acattgatcg taggtgggat aaattgatgt tctaatgcca
    20101 gcaaagttgc caaggcttcg atcccgccgg ctgctcccaa taaatgtcca gtcatcgatt
    20161 tcgtgctgct gacgtaagta tctctgaaat tttcacctaa tgcgatttgg attgcttttg
    20221 cttctgcctg gtcattggct ggtgtacttg ttccgtgagc attgatatat ccgacttgtt
    20281 ccggctgtac accagcttct tccatcgcaa gcttcattgc tttagacgca ccagaaccat
    20341 ctggagtagg agctgtcata tggtaagcgt cacagtttgc accgtatcca atgatttctc
    20401 catagatttt ggcaccgcga gcttgtgcat gttctaaaga ttcaagaact agaacacctg
    20461 ctccttcacc catcacaaaa ccatttcgat tttcgtcaaa cggaatcgaa ccacgattag
    20521 gatcagttga ttctgagagt gctgttaaag ctgcaaatcc agcaatcccc atctcacaaa
    20581 tcgttgcttc tgttcctcca gcaagaatga catcagaata gccatgtttg atgttacgga
    20641 atgcttcccc aattgaattc gttccgctag cacaagctgt tacgatagct gtacaaatac
    20701 cttttgcacc tacgcgcaaa gcaatatttc cagctgccat gttggcaatc gccattggta
    20761 cgaacattgg agaaactcgt tttgcacctt tttcatgtag acgaataact tggttttcga
    20821 ttgttggaag accaccgatc ccagatccga tgatgacgcc aaaccggtct tgattcattt
    20881 tttctttatc taaaccgctc atctccattg cttctaaagc agcgtagatt ccatagatcg
    20941 aaaacatatc cattcgtttg ctatcttttt taataaaata tttatcgtaa gggaaatctt
    21001 tgacttcacc cgctaaggta atccctgttt ctgaagcatc aaatttagtg atcggtgcga
    21061 tgccattcgt cccctctttt aagctattca aaaagctgtc tgcgtcattt ccaataggtg
    21121 aagtgactcc atagccagta attaccactc gattcatttg tttgctctcc ttttcttatc
    21181 cgtgcatgac caatccgcca tcgacattga tgacttgtcc ggtgatatat ggattttgtg
    21241 ctaagaaaac tgctgtttgt gcaatatctt ctacttgtcc gaaacgctgc atcggaattt
    21301 gcttttccgc ttgttccttg actttgtctg ctaaaacttc cgtcatatca gttgtgatga
    21361 atcctggcgc aatggcatta catgtaatgc ctctagttgc agcttctcgt gccacagatt
    21421 ttgttaatcc tactacgcca gctttactag ctgcataatt tgcctgacca atatttccaa
    21481 tcaacccaga tacactagag agattaatga tggccccttc tcgctgcttc atcatttttt
    21541 tcaatacgtg ctgcgtcata ttgaaggttc caatcaaatt gatatcaaga cattttttga
    21601 aatcttctgc atccattcgc atcacaagtt tgtcatttgt gattccggca ttgtttacta
    21661 acacatggat cgatccaagc ttttcttctg cttctttgat catttgtccc gctttctcat
    21721 aatcagagat gtcaccggaa actcctacgc attttacacc gaatgcttcg atttcttcta
    21781 tcttttcttt tggaatctct ccgcgaccat tcaaaatgat atttgctcca gcttttgcaa
    21841 aagctaaggc catcgctttg ccgattcctc ttgtactacc tgtgataaat acattttttc
    21901 cttttacatc cataacgttc ctccttatag tattgctatt gtttcatcaa atgtctgctt
    21961 attctcaaca cgcagaactg gtattgtttt atcgattttt ttcataaatc cgcttaatac
    22021 ttttccgggg ccgacttcga tgacttgttc gatgccgatt gtttttaact tatggatact
    22081 ctcatagaaa cgaacaggtt gcatgacttg ctgttcaagt agtcccgcga ttgtttcttt
    22141 cttcattatt tccgttgttg tgttgcttat gataggaaaa gaaggttcag agaattgaat
    22201 ctgacttagg tccttcgcca gtttttttgc tgccggttct aaaatagctg tatgaaaagg
    22261 cccgctgaca tttagaggga tcattctttt gaaccctttt tcttttaata gtaaaacagc
    22321 ttcgtctact gctttttctt cgccaccaat cacgatctgc tgaggtgtat tataatttgc
    22381 aggagatacg atgccgtact tgctagcttc atgacagctt tcctcgatcg tttcaatcgg
    22441 cgcattcatc actgcaacca ttttcccgct tccagctggg gcagcttctg tcatataagc
    22501 gcctcttttt gctactaacg caactgcttc agtgaaggat agcgcattac tagctactaa
    22561 tgccgaatat tctcctaaac tcagtcctaa agctgcatct gggatgattc catgctcctt
    22621 caataagcga taatacgcga tgctgactgt tagaatagct ggctgtgtat attgcgtctg
    22681 atccaaacgt tcattttctg taaagcaaag ttcagccata tcgtagccga gaatctcact
    22741 tgcttcatca aatgtctgct ttacaacggc ttcttcatat aaatcttttc ccataccttg
    22801 atattgtgca ccttggccac taaataagaa tgctgttttc atgtttttct ccaatatatc
    22861 cagaatatat gtttgattct tcctattcaa aaaggccgag acattcctat ttcaggaaat
    22921 gtcccagccc ccaacctaat tttaatgatt atgcagtttg tttttctacg taagtcacta
    22981 aatcttgaac cgttttgatt ccgtcttctg tttcgatttt cacgtcaaat tcatcttcaa
    23041 tttcattgat gatttggaat aaatccaagc tgtcagcttc taagtcttct tggatgtcag
    23101 tagtcaattt gatttcttca gtttcttttc ctaattcttc tgcgatgatt tcttgaattt
    23161 tttcgaatac cataataata tattcctccg atattgtttg ctttttattt ttataattta
    23221 acagtctaca actccatgag aatacttccc catgttaaac ctccgccata gcctgtaaat
    23281 attactttct gctgttttcc caaggtgatc tttccactag aaactgcttc atccaataag
    23341 atagggatgc ttgcagcaga tgtattccca tatttatcca tattggtcag gaacttttct
    23401 tgcggcatct ttacttttcg ggcaattttc tcaataattc tgaggtttgc ctgatgcaaa
    23461 agcagataat caactggttt gtctttcatg acttcttgga tattgtttgc aacgtctcgt
    23521 acggcaaaat caaaaatatc tctgccagtc atttgcaaat aatacgaacc ttctgaatct
    23581 tctgaataga acggattttt attttgctgg tagccagcgg tcaatgagct acttctggta
    23641 ccgtcagctg ctaatttttc tttcagcaat tttttctgtg gattcgcttc taagagaatc
    23701 ccacctgctc catcgccaaa caacacagca gtcgtacgat cagaccaatc gattagctta
    23761 gatagtgttt caccaccaat cactagtccc ttagttgagc cgcagcggat cattttttca
    23821 gccatgctta acgcataaac aaatcctgag cacgcagcac tgatatcaaa agcgatagca
    23881 tgagttgctc ccaatttccc ttgtacttgt gctgctacag atggcgtcgt gtaatcgggc
    23941 gacattgttg ctactaaaat aaaatccaaa tctttagggt ttagattttt ctgttttatc
    24001 aatttttccg ccactaaaat gcataaatca gaagtctcct gatttgttac acatcttcgt
    24061 tcacgaatac ccgtccttga cgaaatccat tcatcagagg tatccatcat cttggaaaga
    24121 tcattattag taataatctt ttcaggaagg acgctggctg ttgcagtaat cattccaaat
    24181 tgttccatta aatatcctca cttatagtct tgcaagaagt cgtgcaagtt tttcagtgca
    24241 tccagcaagg catgctgttc ctccgtagcc attccgtcta agatgttctt gaccatttca
    24301 cgatgaaaat gctgatggac ccgaaatagt aactttcctt ttttcgttag tcccaacttt
    24361 acaacgcggc ggtcatcttc actgcggata cgttcgacat agcctttttt tactaacttg
    24421 ttaatagcag tggtcaaggt cccaacagta attgatagtt ctttggctac ttctgaagtt
    24481 gtttttttct tgtacatccc gattgcctcg atcgtgtgca tctctgtgat tgacagatct
    24541 ttgaattgag attttttcag ctcagactct tcgattgtca aaatatcatt gaagacacta
    24601 acaagataat catttactgt ttttaaatct ggttccatgt tccaccgtcc actaattagt
    24661 ttgattatca aatcttttga tgttcaaact atatcttaac ttttttcaaa atgcaacctt
    24721 aaaagatcat ttttttacta tatgagcttt ttttaagatt ttactcattt aaaacaactg
    24781 ataaatcagc atttataatt tcttttatta gaaaaatcat ctatctcaca tattttgatt
    24841 atcaaagtat tttctgtgaa cagaactata gaattcctta aaacttgtcc attatttcaa
    24901 aatttgttat agtcttttta ttcaaagcgt aaaaggagat cctatgttaa aagaaaaaat
    24961 gaaaactctc cccatgctgc cgggtgtcta tcttatgaaa gaccgtgatg gtaaggtcat
    25021 ctatgttggt aaagcaaaac ggctcaaaaa tagagtaagc agctattttc atcagaataa
    25081 acagcattca aaaaaagtat tacgtatgat tcatcatata acagattttg atttcgtcgt
    25141 agtagacacg gaattagatg ctttattgct cgagtgtcaa ttgatccaac attaccgtcc
    25201 tttttataac cgacaaatga attatttcag taattataat tatgtacata ttaccaataa
    25261 gggatttgta ttaacagata ctcctacggc tcgaacatac gggccttttc gtctctataa
    25321 aaaaatgccg tctattttgc gtataatgga agaaacttat caaatgcctt ggttgtctga
    25381 aattagtttg ttagctttgc gagcccagct tcctgaccta caggaaatga cttttgaaca
    25441 gaaaaaaaag gaactccaag ggctgtttca aggccaaaat aaaaagctac taacttactt
    25501 gaaaaaaaga caacaacatt tcatctatca gttgaatttc gaaaaagccg gtatgcttca
    25561 aaaagatatc gaattagtta cttattttat tagacggatc caggaacaaa agcatttttt
    25621 aagaacacca tcgctcacct tcagcatgcc attagcagca gatgagagtc agaaaaagca
    25681 ctacctcgtt tgttatggtc aacttgcaga aacaatgatt gcctctgctg acgtttctcc
    25741 tgacttctat tatgaaaaga aagaagctca tttatctttg aaacgtcaat tgtcaaaaga
    25801 agaaatcgat cctgtacaaa tcttgatcag ttaccgcaaa aaattagaaa aagaacagtc
    25861 tgaaattgaa ctgttaaaca aaaaagaagc tgaaaagcaa ctaaattagt tgcttctcgg
    25921 cttcttttag gcatcttcaa cttcaaatgt caattatcct aaaatttatt ggattaagtc
    25981 atagatttcc atcgctacga tatcgatatt atcaaattcg tattgctgat gagtatctgt
    26041 ttcagttaat tcaaatgtat cggtcgcttt atcataagtt actgtacact tctctacgcc
    26101 ttctttttca aaacggcgaa cttgcagttc attatctgct gactcgacca tagcctccag
    26161 tcgttttatg attgcgacta attgtgaatg tgtcatcgga ggtcctcctt ttcttatttc
    26221 tgttatattg tatcaaaagc atagcctact tgcattgatt ttctctaaaa aaactcgttt
    26281 tttatgattt tttttctggt atttttcctt caggccacca aagtttgatc aaagcttggg
    26341 ttccgtcatt ctcaaaacct ttatctgcca actcttcata tagtttcaat gcttgggtag
    26401 ttgctggcaa tacaaggtcc attttttctg cttcttctaa tgcaatcttt aaatctttga
    26461 taaaatgttt gacaaaaaaa ccaggtgtgt aatcttcttt taaaatacga ggagaataat
    26521 tgcttaaaga ccagttagct gcacttccac ctgaaagcgt ctcaatcact ttctttaaat
    26581 caagtccgtt tttttgtgca tagacaagca tttccgtcat tcctgtcatc gtacctgcaa
    26641 tcatgatctg attagccatt tttgtatgct gacctttccc agcagaacca tgcaatgtaa
    26701 atgtcgtacc aaattctttg aataaaggca gtacttgatc ataaacggct tcttctccac
    26761 cgaccatgat cgtcagtgta ccattctttg ctcctaagtc accaccggat accggtgcat
    26821 ctaacgcatc tgcgcctttt tcttttgctg tttttgcaat tttttctgcc aggcttggtg
    26881 tacttgtagt caagtcaact aaaatatgtc ctgagatatc tgcagaaaaa atccccgact
    26941 gtccatagta gatttcttct acatctttgg ggtaacctac cattgtaaag agaatattac
    27001 tttgttctgc taccgctttt ggtgtatctg cccagatagc tccttctgtt actaaaggat
    27061 cagcttttgc ttttgtccga ttatagacag ctacttcgtg tcctgcttct agtagatggc
    27121 gtgcgacagc acttcccatg acgcctgttc cgataaaacc aagtttcatt ttttcgacct
    27181 cctaaaaatc aaaaatcctt gcttctatgc tagtatacgc tagaaacaag gattttttca
    27241 taaaaaatgt ttgaatttta tcttttaaat gaatttaatt ttattttgat tcgttcaatc
    27301 gagtcttctt cttcttttct tcgcatctct tcttcatgat aaacaaccat gtcttgataa
    27361 aattcgatca tagcttcgcc gaaatgtgta gatgaaatat cataaagttt agcttctaaa
    27421 acttttggat ctttaggaac ttcactggaa atataatcaa taagtgctgg tgcaaagtct
    27481 tcgtcggttt caaaagtttt ccctaatgaa ggatgatcaa acaatcggtt caaataagca
    27541 tttccttcca cgacacaagg tgttccggct gccatcgctt ctgtataagt aagcccttgt
    27601 gtttctgaag tagaagcact gacaaaataa tctgccgctt tataataaag tgctacatca
    27661 ttgttgtcca cttctcctgt gaactgcaca aattcttcta actgcagttc tgagatcaat
    27721 tcctctaact cttctacata aggtcctttt cctacgataa ctaatctggc gttaggataa
    27781 gcggagatga cctgaggaaa cccattaatc aaggcttgga tatttttttc ataagaaatc
    27841 ctgcttaaag aaagaatcat caaatggttt tcctgaagac ctagtgactc ccttaatttg
    27901 ctgatatcct gctgagtgat atcagaacgt ttgaattttt caatgtcgat accagtagga
    27961 atcacgcgaa gcggtgctat tacaccatag ctacgtaatg tatcaataac acgttcactt
    28021 ggacaaacta cacctgtcgt atgatttgca aataatctag acaagtattt tacatgggat
    28081 tgcctgatca cttttccttt accgatataa tgaaggtagt cttcatacat cgtatggtaa
    28141 gtatggatca gaggtatttt caacttctta gctaccatct tcccaagtat ccctgctccg
    28201 aattctgtat gggtatgaat aagatctagt tctagttctt tggcaaccaa ataagcatac
    28261 cacatgcctc taacaacaac tcttcgatcc ttgaaagaaa taaatggtac acttggcatt
    28321 cgaatcacat ctttttccaa cttatccgca ttaggatcgg tcgttgtaaa aatatagact
    28381 tcatgcccat gtctttctaa ttcttgtttt aatgttttga tcgatgtagc aactccgctg
    28441 acttgcggaa agtaggtatc ggtaaaaaag ccgattttca tgtttttcgc ctccaaacta
    28501 gtccttgacc tgatttactt tttttaattt gctagagact ttttcattca gtacactttc
    28561 gtatacattt ttaagttctt caccgatttt attgatactt cgctcttttg ctgtttgata
    28621 accggcttgt gtcgtactcg gtagttcttg attggtaatc tgttggatca gtcggataaa
    28681 ttcttcgttt gtcttcccca tatagcagtt cttgctatct tctaaccagc cattatacac
    28741 tggaatatct cgaacaagta cgttttgttt gcttgctaaa gcttctaaaa caacgatccc
    28801 ttctgtctct tcataagaag ggaaaaagaa tagatctgca cctgaatacg caccttctat
    28861 gatctctcca cgaatgtaac caggaaacag gacattttca ggatgatctt cttttacaat
    28921 tttccggatg ttccgaggaa tcgagtacat gggaacatga ccaaaccaaa taaatgtgta
    28981 ttcgggcatc ttcttagcaa tttctacgaa atcaatgatt cctttacgtt cgaaaaacaa
    29041 accgacacaa atgattactt tttgatccgg agacagtttg aaatattctc tgaacttttg
    29101 ttctttcatt ggatcagggc gatatttctc tacgtcgatc ccattggaaa tagcttgaat
    29161 cggcacacgg atactatagc tttccaacaa ctgttttgaa taaggtgttg gcgtgatcag
    29221 atagtccgct ttttggtaca atcctaccag atattttttt acgataggag aaagctggtt
    29281 cgatccgata aaggaatttc ggaaatcttc ctctgtagaa tgcgcatgat agacaacttt
    29341 ttttccttct cttctggctt tattgaccat attatgacta ttgattccgt atgtattgat
    29401 gtgcaggatg tcataatctt ctttttcatc tagtgtataa gagataccga cttccgtcag
    29461 agcacgtttt tgatgatcaa gcgcacgccc gattccagat tttgcaagca tcttttcact
    29521 ttcaaaatat aataatacct tcaccttacg tcccccttca cttatatagt ataacatagc
    29581 ctacaaactg tcactcaaac ttaagcccta tttttatcta ttcttactga caaaaacgtt
    29641 ttcttaaaaa agaacaaaaa aactggcgta gctcttttac aagccacacc agcttttgat
    29701 acgttgttta ttttacatat tcatgaacaa gttcaacgac ttcttccatc gtatcgcatt
    29761 ctttcaatgc acgatcagca agttcagcca ttttcgctgt gtccaagcgt ttcattaaac
    29821 tacgcgtttt aagaatagat gtcgcactca ttgagaactc atctagaccc atacctacta
    29881 gcaatggaac agctgtttga tctccagcca tttctccaca catacctgcc cattttcctt
    29941 cagcgtgtgc tgcatcaatt acatttttga tcaaacgtaa aattgatggg ttatatggtt
    30001 ggtacaagta agaaacgcgt tcgttcatac ggtctgctgc cattgtgtat tgaatcaaat
    30061 cgttcgtacc tacagagaag aaatctactt cttttgcaaa cttgtctgct aagactgctg
    30121 ctgctggaat ctcgatcatg atgccgactt ggatcgtatc agatacttcc ttgccttcac
    30181 tgatcaattt ttgtttttct tcttcaaaga tcgcttttgc tgcgcggaat tctttgagtg
    30241 tagctaccat agggaacatg atacgtaagt taccatgaac agatgcacga agcaatgcac
    30301 gcatttgtgt acggaacatt tcgtcgcctt gttcagataa actgatacgc aatgcacgat
    30361 atcctaagaa tggattcatt tcatgaggta attgcagata tggtagttct ttatctccac
    30421 cgatgtccat tgtacgaaca actacaggtt ttccttccat accttctaat acagctttgt
    30481 aagcttcata ttggtcatct tctgttggga agtctggaga atccatatac aggaattcag
    30541 tacggtacag accaactgct tctccaccat tattatggac accaactaga tcttttggtg
    30601 ttccgatgtt tgcagctaat tcaaaatgct tgccgtcagc tgtgacagtt tctgcatttt
    30661 ttaatttttc ccattctgct ttttgtgctg cgtaatctgc acctgctttt tcaaattcag
    30721 ctttttgttc atctgtagga tcgatgatta catcgccttc gatcccgttg acagccaaaa
    30781 tatcgccttc tttgacttta gcagtaattt cttttgtacc aacgattgct ggaatttcta
    30841 aagaacgtgc catgatagca gaatgtgaag tacgtccacc aatgtctgtt acaaacgctt
    30901 ttacaaaatt gcgatctaat tgtgctgtat cacttggagt caagtcatgt gcaacaacga
    30961 ccacttcttc gttgatcatt gatggatttg gtaaagtaac gcctaataag tgcgctaaaa
    31021 tacgtttagc aacatcacga atatctgcag cacgctcttg catataagca ttatcttcca
    31081 ttgcttcgaa cataccgata tacatatccg ttacttcttt tagcgctgat tcagcattta
    31141 cgctgttgtc tttaatattt tgcttgattt gacctaccat ttctggatct gaaagaacca
    31201 tcaaatgggc atcaaatact tgcgcttctg cttcacctaa gctttgcgct gctttttcac
    31261 gaatttgctg caattcttca gtagatttag ctaatgcagc atccaaacga gcttcttcag
    31321 ccgcagtatc ttctactgtt gccttactga atgttaaatc cggttgaact agcaggtaag
    31381 cttttgcaac ggctacaccg tcactagcgg caatcccttt tagcatttca accattattc
    31441 agataagcct tctttcttca ttgtttcaac gattgctgcc attgcatcag cttcgtcagc
    31501 accttctgca gtaatagtaa catcagaacc ttgacctaca cctaaagaca taacgcccat
    31561 gattgatttc aagtttactg atttaccttt atactctaag tttacatcag agttaaattt
    31621 gcttgctgtt tgtactaata gtgtagctgg acgagcgtgg atcccagttt ctgctactac
    31681 gtgaaattct ttcttttcca tattaatcgg tctcctttaa gtgagtgttg tttttatgtg
    31741 agggttttct tttttagtaa ttcagagaat cctaacaaat gccacccttt catctgtaaa
    31801 gattaacatt ttttttcgtt agatacaaca gttttcattc aatttcagat aaaagtattc
    31861 aaattattcc ggattctttt tcatcctttt cttcacattt tataacaaaa gtgtttattt
    31921 tctttttctt gtcgctttct cttctcctga cgtttatcat tctttccttt gaccctcttt
    31981 gttttcatat gaaacgaatt acttgcttcc ctttttattt ttagtataat aagagtcaaa
    32041 ggtcaggaat ggtcaataga gatttcacct tttctacctc ataaatctct taaaaaacta
    32101 ggggttattc ttttacctca tgaaaaacga gatgtaaaat atggatgtac ccatttttgt
    32161 gaaaggacgt gaatgattca tgctttgtca aaactgcggc aagaatgagg caacgattca
    32221 cttgtatgcc actgtcaatg gtcagcggac acaactcgat tactgtcaga gctgttatca
    32281 aaaaatcaaa aatcaacaaa atggaggtct gactaatatg gcccaaaatg atccatttgg
    32341 atttggcagt ttggacgatc ttttccgttc catgtctcgc caaatgcaac agcaaggaaa
    32401 ttatgagcaa acaccaccaa cacaatttgg cggaaacaac aacttcaatg gtggtcagcc
    32461 acctcaaggc aatacagaag gtttattggg agaatacgga atcaatatca cagaacaagc
    32521 acgacaaggt gatatcgatc ctgtgatcgg acgcgatgac gaaatcaaac gagtcattga
    32581 aattttaaat cgccgaacca agaacaatcc tgtcttgatt ggagaaccag gtgttggtaa
    32641 aacagcagtt gttgaaggtt tagcacaaaa aatcgtagat ggtgatgtac cgcaaaaatt
    32701 aatggacaaa gaagtcattc gtttagatgt cgtatcttta gtccaaggta ctggtatacg
    32761 aggacaattt gaagaacgta tgcaaaaatt gattgaagag atccgtcaag cagaaaatgt
    32821 gatcctattc atagatgaag ttcacgaaat cgtcggtgct ggttctgctg gtgatggaaa
    32881 tatggatgct ggtaatattt taaaaccagc tttagctcga ggagaattgc aaatggttgg
    32941 tgctactacc ttaaatgagt accgcatcat cgaaaaagac gctgctttag aacgtcgtat
    33001 gcaacctgta cgggtagatg aaccaactgt cgaagaaacg atcagtattt tgaaaggttt
    33061 gcaaaaacgt tatgaagact atcatcatgt aaaatatact gatgaggcca tcgaagcagc
    33121 cgcaacttta tccaatcgtt acgtccaaga tcgcttcttg ccggataaag ccatcgattt
    33181 gctagatgaa acaggttcta agaaaaactt gacgatccaa atcgttgatc ctaaaacaat
    33241 cgagaaaaaa ctgcaagaag cagaagaaca aaaagtttta gcttctcgag aagaagattt
    33301 cgaaaaagca gcttattatc gcgatcagat caataaactt caaaaaatga aagaacgtca
    33361 gctcactgaa gaggaaacac ctgtgattac cgaaaaagat atggaaaaaa tcgttgaaca
    33421 acggacaggt attccagtgg gtgaattaaa agaaaaagaa cagacacaac tgaaaaactt
    33481 agcagatgac ttgaaagctc atgtcatcgg acaagataac gctgtagatc gagtagcgaa
    33541 agcgattcgt cgaaaccgtg ttggattaaa caaacagaat cgtccaatcg gctccttctt
    33601 atttgttgga ccaactggtg taggtaaaac agagcttgcg aaacaacttg cctacgaact
    33661 attcggttca caagattcaa tgattcgttt cgatatgtct gaatatatgg aaaaacatag
    33721 tgtctctaaa ttgattggtt ctcctccagg ttatgtcgga tacgaagaag ctggtcaatt
    33781 aacagaaaaa gtacggagaa atccttacag tcttgtcttg ttagatgaag tagaaaaagc
    33841 tcatccagat gttttgcata tgttcttgca aatcttagat gatggtcgat tgacagatgc
    33901 acaaggaaga acggtcagct ttaaagatac gatcatcatt atgacaagta atgccggaac
    33961 tggtaaagta gaagcaaacg ttggttttgg tgcggcacgc gaaggtgtta ctcgttctgt
    34021 attgaaccaa ttaaacaact actttacacc agaatttctg aaccgttttg acgggatcat
    34081 cgaattcagt gcattatcaa aagaaaactt gatgacaatc gtcacactaa tgctagacga
    34141 tgtcaaccaa atgctggcag cacagcaact tcacatcgaa gttcctacga atgtcaaaga
    34201 aaaattagtt gacttaggct atgatccatc tatgggtgct cgtcctcttc gacgcacgat
    34261 ccaagaacaa atcgaagatg gtatcgctga attttatctt gatcaccctt ctatccatga
    34321 gttgaaagca aaactggata aagatggaaa aatcatcgtt acttctaaac cagaacgttt
    34381 ggctaaagaa tcagccgaag aaacggctga ataaatccag ttttatagcg tgagacggta
    34441 tttctttgag aaatgccgtc tcattctttt ttcagaaaag tcaaaatact gttcatttta
    34501 taaaaaagaa attataataa gagaaacaag caattaaaaa tacataaaag aggtgagcgc
    34561 catgaaattg gtaaatgtaa caaacagcca ttcccgtctt gtattaaacc agcttgaaaa
    34621 cactgatgcc catctagtca aggtctacac agccggaaat accactattg tctacactga
    34681 agcccctgaa cataatgaaa ttctgctgat caatgacaaa cgtaaaatcc agccgaaaga
    34741 aatcgaagat gctaaaaatt atttcttaga taaaatcaaa gacgccgttt accacgagga
    34801 tgaaatcaaa gtaatcgaat tggcaggcct tgttgaaatc tcgattccaa aacatgaatt
    34861 aggcgctaca ttagccgtgt aaacaaattt tgaacataat aat
//
LOCUS       DAMEET010000021        34660 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.021, whole genome shotgun sequence.
ACCESSION   DAMEET010000021 DAMEET010000000
VERSION     DAMEET010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 34660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.021"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(313..2205)
                     /locus_tag="PB780_000872"
     CDS             complement(313..2205)
                     /locus_tag="PB780_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDE0732943.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2202..3929)
                     /locus_tag="PB780_000873"
     CDS             complement(2202..3929)
                     /locus_tag="PB780_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDE0732944.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3945..4595)
                     /locus_tag="PB780_000874"
     CDS             complement(3945..4595)
                     /locus_tag="PB780_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDE0732945.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4799..5095
                     /locus_tag="PB780_000875"
     CDS             4799..5095
                     /locus_tag="PB780_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDE0732946.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5287..5907
                     /locus_tag="PB780_000876"
     CDS             5287..5907
                     /locus_tag="PB780_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDE0732947.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5999..7165)
                     /locus_tag="PB780_000877"
     CDS             complement(5999..7165)
                     /locus_tag="PB780_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDE0732948.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7177..7530)
                     /locus_tag="PB780_000878"
     CDS             complement(7177..7530)
                     /locus_tag="PB780_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDE0732949.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7658..8680
                     /locus_tag="PB780_000879"
     CDS             7658..8680
                     /locus_tag="PB780_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HDE0732950.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8699..8860
                     /locus_tag="PB780_000880"
     CDS             8699..8860
                     /locus_tag="PB780_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732951.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9119..9553)
                     /locus_tag="PB780_000881"
     CDS             complement(9119..9553)
                     /locus_tag="PB780_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732952.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9663..11522)
                     /locus_tag="PB780_000882"
     CDS             complement(9663..11522)
                     /locus_tag="PB780_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDE0732953.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11640..12799)
                     /locus_tag="PB780_000883"
                     /pseudo
     CDS             complement(11640..12799)
                     /locus_tag="PB780_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            complement(13106..14530)
                     /gene="araA"
                     /locus_tag="PB780_000884"
     CDS             complement(13106..14530)
                     /gene="araA"
                     /locus_tag="PB780_000884"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HDE0732954.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14546..15244)
                     /locus_tag="PB780_000885"
     CDS             complement(14546..15244)
                     /locus_tag="PB780_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDE0732955.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15201..16862)
                     /locus_tag="PB780_000886"
     CDS             complement(15201..16862)
                     /locus_tag="PB780_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HDE0732956.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17080..18480
                     /locus_tag="PB780_000887"
     CDS             17080..18480
                     /locus_tag="PB780_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HDE0732957.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18732..19811)
                     /locus_tag="PB780_000888"
     CDS             complement(18732..19811)
                     /locus_tag="PB780_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDE0732958.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19897..20856)
                     /locus_tag="PB780_000889"
     CDS             complement(19897..20856)
                     /locus_tag="PB780_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDE0732959.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20868..22379)
                     /locus_tag="PB780_000890"
     CDS             complement(20868..22379)
                     /locus_tag="PB780_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HDE0732960.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22401..22964)
                     /locus_tag="PB780_000891"
     CDS             complement(22401..22964)
                     /locus_tag="PB780_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HDE0732961.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22970..23833)
                     /locus_tag="PB780_000892"
     CDS             complement(22970..23833)
                     /locus_tag="PB780_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDE0732962.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23847..24743)
                     /locus_tag="PB780_000893"
     CDS             complement(23847..24743)
                     /locus_tag="PB780_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDE0732963.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24918..26207)
                     /locus_tag="PB780_000894"
     CDS             complement(24918..26207)
                     /locus_tag="PB780_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDE0732964.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26179..27696)
                     /locus_tag="PB780_000895"
     CDS             complement(26179..27696)
                     /locus_tag="PB780_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HDE0732965.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27894..28784
                     /locus_tag="PB780_000896"
     CDS             27894..28784
                     /locus_tag="PB780_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HDE0732966.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28857..29681)
                     /gene="nadE"
                     /locus_tag="PB780_000897"
     CDS             complement(28857..29681)
                     /gene="nadE"
                     /locus_tag="PB780_000897"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDE0732967.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29693..31165)
                     /locus_tag="PB780_000898"
     CDS             complement(29693..31165)
                     /locus_tag="PB780_000898"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDE0732968.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31802..32164)
                     /locus_tag="PB780_000899"
     CDS             complement(31802..32164)
                     /locus_tag="PB780_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HDE0732969.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32387..33139
                     /locus_tag="PB780_000900"
     CDS             32387..33139
                     /locus_tag="PB780_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDE0732970.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33279..34202)
                     /locus_tag="PB780_000901"
     CDS             complement(33279..34202)
                     /locus_tag="PB780_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDE0732971.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34217..34489)
                     /locus_tag="PB780_000902"
     CDS             complement(34217..34489)
                     /locus_tag="PB780_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732972.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10230 a         7212 c         5979 g        11239 t
ORIGIN      
        1 caagaggttg tgagcctgcc gtttaactcc gagtcacaag gagcaatttc aaaaaacagc
       61 ttctcaggtt tgttgactat tgtgacttgt gaccgaggag ttggcaggag aacaccgttt
      121 aaggaagagg ttgtaaaatc agcggtttga cctgagtatt agagatacaa aacggaaaaa
      181 tagcttctca tattttgccg ttttttgagc taataccgaa ggacagctga ttgaacaccg
      241 tttattcgga aaaagggact gtgacacgac ttttgtcaca gcccctaaaa aaaataataa
      301 atttgtacta ttctatgaag cttcttcttc agaaaactga ctgttataaa gatctgcata
      361 gaatccgtct ttttccatca atgtatcgtg gtttcctgtt tctacgatcg aaccatgatt
      421 catcacaata atgttgtctg catcgcgtat tgtagacaag cggtgagcaa caacgaaact
      481 tgtccggttt tccaataggc ggttcatcgc tttttgaatc agtatttctg ttcgtgtatc
      541 tacactagat gttgcttcat caaggatcag gacatctgga tttgctaaga atgcccgcgc
      601 aattgtgatt agctgacgct gcccttgcga aatattgctt gcatcctcgt ttaatacagt
      661 gttatatcct tctggcaatt tacgaacaaa atcgtctacg tgagcagctt tagcagcctc
      721 aatgatttgt tcgtctgttg catcgtcgtt cccatatttg atattgtcat agatacttcc
      781 tgtaaacagc caagtatctt gcagtaccat tgaaaactgt gcgcgcaatt catcacgtga
      841 aagatcacgg gtatctacac catcgtaacg aatactacct ccagaaacat cgtagaaccg
      901 ttcaagaagg ttgatcagtg tcgtcttccc tgcaccagtc ggacctacaa tagcgaccat
      961 ctcaccaggt ttgacatcca agttaaaatc agtcattagc aagttatctg gtgtgtagcc
     1021 gaattgaaca ttttcaaaac gaactttgta tggtgtatct ttttcttctg gaaggccaga
     1081 tttttcattg gacatctctt cttcatccaa cacttcaaat acccgttcgg ctgaagcaac
     1141 tgttgattgg atagtgttca tcaaacttgc aagttgagtg attggttgag agaactgtgt
     1201 tgtatattgc aagaacgctt gaacatcccc taatgtcatg ttcccatttg caacttttac
     1261 tccgcctagt acagcgacga acacatagcc tagattttta acgaaattca tcaacggcat
     1321 gatgattgcc gaaataaatt gagctttcca accagcggca tacagattct gattttcttt
     1381 ttcaaatact tcctggtctt tttcagtatg attgaatgtt ttaacaatgg tatgaccacc
     1441 atatgtttct tctacttggt tattcaataa acctaaactt ttttgctgat ctgcgaaaaa
     1501 cacttgtgat ttcggcgcta cgatcatcac gatgatcaag cttaaaggga tcatcaataa
     1561 cgcgactaac gtcaattgcc agctgattga aagcatcatc acgatgaccc caatcaatgt
     1621 gactgcacta gttacaaatt gagtcaaatt ttgctgcagc gtgcttgcga tgttatccat
     1681 atcattgatt gctcgagaca tgatatcccc atttgtatgt gtatcataat aggaaatcgg
     1741 tacacgattc attttggctt ccaaatcatg acgtaattca taaactgttc gttgggaaac
     1801 acgtgtcata ataaattgct gtaaaaagtt gaatacagca gaaatcaaat acatcacgat
     1861 tactgtcgca atgatctgcg cgattttatc aaagtctatc ggaaattttg ttacttgttg
     1921 gccttgttgc atctgttgag cgccagccat tacacctttg aatatttctg tggttgcttt
     1981 ccccaagacc tttggtgttt ggatttggaa gatcacggct gctattgcta gtacaaatac
     2041 agcgatgatc gcaaccatac gtttagacat atagccaaac aatcggcgta ccgttcccca
     2101 gaaattttta ggcttttcac cttttacacc gagatttctg ccaggtccac catgtcctgg
     2161 accgcctgct ggtccatgag agggacgtgt gtttgtttta ctcatgctaa gtcctcctct
     2221 cttagttgtg aatgaacgat ttcttgataa gtttcgtttg ttgctaatag ttcttcatgt
     2281 gttcctttgc caaccagttt tccttcatct agcacaagga tcatatctgc atccattact
     2341 gtacttactc gttgagcaac taaaacggtg atactttctt tcatattcgt tttcaatgct
     2401 tggcgaagat tggcatccgt tttgaaatcc aacgctgaga aggaatcatc aaacacatag
     2461 atatctgctg ttttgactaa tgcccgtgca attgccagac gctgtctttg tccaccagag
     2521 aagtttcctc ctccttgttc cacatggcta tctaagccat cttccatctc tgaaacaaaa
     2581 tctttagctt gtgcgatttc taatgctttc caaatcattt catctgttgc atccggctta
     2641 ccatactgca tattgtctcg gatcgttcca gagaacagaa ctgctttttg aggaacaaag
     2701 cccattaatt ctcgcaaatt atattgagtc atgtcactaa tgtccgttcc atttatttgg
     2761 atcgaaccag attcgatgtc atataatcga gggatcaaat tgactaaggt cgttttacct
     2821 gaaccagtcc cccctatgat tgcgacgatc tcgcccgact tggcttggaa atcaatatct
     2881 tccaatgcaa gtttttctgc tccatgatat cgataattga catgattgaa ggccaatgtt
     2941 gcgttttttc cagcaaaaga aactgtttta ggattttcag ggtcatggat accgattttt
     3001 tcatccaaca cttcattaat acggtcagct gaagcttgtg cacgaggcac catgataaaa
     3061 atagcggaaa gcatcataaa gctgatcaag atctgcatcg cataagtcat aaatgcaatc
     3121 agattaccga cttccaaagt catatctgct atgtagtgtc caccaaacca agtgattgct
     3181 acgtttgtac cgctcatgat caatgtcatc attggcatca tcaatgcgac gatcgtatta
     3241 actttgattg ccgtatttgt ataatcttta ttcgctaaat caaaacgatt ttcttcaaag
     3301 cgtgtcttat cgaatgctcg aatcactctg acccctgtca atccttctcg aaataccagg
     3361 tttaaacggt cagttttctt ttgcatactt ttgaataatg gaacagcaaa gtacatgatt
     3421 ccgccgatca aaataatcat tactgggatg acaactaaga agatttttgt caactgatga
     3481 tctttttgat aagcaagtat gctcgcgcca atcaacgtga ttggtgcatt gatcatcata
     3541 cgtaaaaaca tttgtgtaac catttggatt tgagtcacat cattggtcgt tcttgtgatc
     3601 aaagaggccg ttccatattt atcaaacgca tcttttgtca gactctctga ttttttatag
     3661 acatccgtac gcaattgctt acccagtttc tgtgattcac gagtagcaaa aaatgtattt
     3721 gcgatagcag ctaagatact gatcaaagaa aagccgatca tcacaaaacc tgtatgccaa
     3781 atataatcga catctccttg cgctacccct ttatcaataa tattggaggt aagtgtcggc
     3841 aaatataaat ctgccagtac ttggatcacc atgaaaactg cagctgcaat tgctgaagtc
     3901 aaggagatcc gttttacgat ttttagcatc tagttccctt ctttctattt atatactccg
     3961 tttttcagcc aagaaagttt caaattgaat tcactgattg cttgctctac tgagtaatct
     4021 tcagatgttg aaaataagcg atacgaatgc gcaattgttg taaatacagt attcatcact
     4081 aattggatca gtaattccaa ttcatgatca ttttttactt tcagcttcgt ccgatcgatc
     4141 aattcaagca actcttttgc atcttgtttg tgttctttat gtggatcgtg aggcgttttt
     4201 tggcgactcg tatgaggagc gacttttctt gaggcacggt aatccatgtt cataaacaag
     4261 ttacgataaa aagaagcatt ctcgccttcc aaaatctcat aaatcatttt agaaaaatag
     4321 gattcgaatc ctttgaatag atcaccatcg gattctttca ttagttgtaa catatcttta
     4381 gagctgtcac gtctcattgt ctcaaagtaa taaaaataaa gatcctcttt atcttcaaaa
     4441 tactgataga aactgcctct agggatatcc gctaatttga cgatattagc aatcgaagct
     4501 tcttttaagg gtactctaga aaactcgatt ctcgcagcct cgattagtct tttttgtttt
     4561 tctttaggca aatggaaaaa tgtctgtgtc ggcatatgga ttctcctttc tttcaagatg
     4621 acacattgtc atatgacgct atgtcattat aacacctcct acctgttttg caagacattt
     4681 gcatagtttt tttcattatc atccgataca ttaaaaaagt gataagagga atccttccat
     4741 ttgaattaat gagagttgcc cattaaaata gccacatact aaacaaagga tgagataaat
     4801 ggcccaatca attagtgtcc aagacttcaa taaattgcct ctcgatgctg acagcatcgt
     4861 tttagataca agaaatacct tagattacac aaaaaaccac ttaagccact cgctttctat
     4921 ccccgctgct atcttgccaa gacaattaaa aaatctagat ccaaagaaac tttattatat
     4981 tctaagctat acaggcaggc gatcagaagc attagccgat caacttactg ctaatggttt
     5041 tcgtgctgtt tatatcattg gtgggatgga agctcttcag tcgagtgttg cttgaatatt
     5101 gcttgttaga ttcaaaagag gctgtgacaa aaaatcgtgt cacagcctct tttataccct
     5161 gtcttttttt agttatttat tcttcaacct tatcaatatg acttgatagc cttttctttt
     5221 tcgagtaaac tgattttgtt agtcatagtt ataacaatca tagcaataaa aggagaaggt
     5281 aagcatatgg catctgtttt agtagtcaaa ggtcatcccc taactgcaga agaatcgcgc
     5341 acagttaagg cattgacgtc ttttttaaca agttataaag aaaatcatcc agatgatgaa
     5401 gtgactgttt tggaacttta tcgcgatgat attccggaaa tcgatgaaga attattatca
     5461 ggttgggaag cattacgtgc tggtgcagaa ttcacttcct tatcagaaag tcaacaacaa
     5521 aaaattgctc gtttcaatga attgactgat cagttccttg ctgctgataa aatcgttatc
     5581 gcaaacgcct tatggaatct aaatatccca acaaaattaa aagcttggtt cgataccgtc
     5641 aatgtagctg gaaaaacgtt ccgctataca gaaaacgggc cagaaggatt agtgacaggt
     5701 aaaaaagcac tgcacatcca atctaatggc ggtgtataca acggacaaga ctttgcctct
     5761 caatatgtaa aaggtatttt aaactttgta ggcatcgatc aagtagatca gctattcatc
     5821 gaaggaatcg attatgaccc tgaccgtgca gatgagttga tgcaaaatgc attagataaa
     5881 gctgctgctt taggtaaatc attttaaaaa ttgagataaa tgcatacaaa agagacagag
     5941 gaaaaaatct tcaaagcagc atttgtgcct tggatatttt cccatctgtc tctttttgtt
     6001 attctttctg ctttttcaac ggattttctt cataaagaga agagattgtt gtgtaccatt
     6061 caaaaacatg agtaatcact actttagctg ctgcataaac aggaatcccc aagatcacac
     6121 cgatgattcc gaacaatttc ccagaagtca gcagaactaa taaaatcgtt actggatgga
     6181 ttgctaactg attccctagt actaatggag agatcaagcg tccttctatt gtctgctcta
     6241 ctgcaaaaac gatcagtact ttgatgatca tgaatggacc tgctacaatg gctaaaaaga
     6301 tagccggaat catcgctaaa aatgatccta agtaagggat caggtttaaa aaacccgcaa
     6361 taatccccaa cgtgacagca tactctaaac caatgaccgc aaaaccaatc ataaacatga
     6421 ttgccacagc aaaagccaca gttagttgtc ctcttatata agaagaaact tgactattca
     6481 tttcactcag cacattcaac gtcggttttt gcatgcgtgt cggtaaaaat ttgacaaaat
     6541 aaggtgctaa ttgtttaccg tctttcaata gataaaataa gataaacggc atcgtcagca
     6601 gagccactaa aatagtcgct accgcgccga caaaacttcc aagactctga actgtactct
     6661 tagatatatc ttggatcata tcgcctgccg aattcatgaa cttatcacct gctgtttcca
     6721 actgttcgcg aaattggttg aacagcgggt cccgcaaaat ctcggttaat ttattatcaa
     6781 tcgtatctac atattgaggg aagttactga tgaagctcac tgtttgttcc tgaatcttag
     6841 ggatgatcac tacgctcccc catgcgatta gtgcaacaac caaaacaaac agcccaatga
     6901 tactgtaaag ccttggtact tttcgctttt caaagtaatc aactaccgga ttcatcaagt
     6961 aatacaagat tcctgctaaa atgataggca gacctacgat tgctaaaaat tgccagattg
     7021 gtgcaaacaa atatgaaact tttgtaaata aaaagacgat cagcaatatt aacaatacaa
     7081 caagtaaggc tgtgactact tgattattta aaaaccagcg ccaaaaccac gataccggtc
     7141 tgttcttctt attttttcct tccataattc ctcctgttag ttataaaaaa tttggcttcc
     7201 caaaccaata gcaggcagtc tatgcggatg aagatagatt ccatttgcaa tttgtccttc
     7261 ttctggacaa gcatcaaaaa tcaatgatac gtgagctact gtctttaaat tcccattggc
     7321 catcggaccg acataatcga tcgtatatac ctgatcatca aaagcaattg ttccgcctgc
     7381 tttcaaagaa aaaggttcgt cttttgatac ttcttgaatc accgaatatt ttcgtaaggc
     7441 agctgttgct gttttatcga acaaaatcag catgggttct tccttgttta ttgcttctgt
     7501 cccaatttct gtaatctttg cctgaatcat tgcactctct cctttaattg ctttcaaaaa
     7561 cagtataaca cattctcatt ctttagccgt gtggaataat tatgtgatag aataaaaacg
     7621 aaaactattt cattacaatt aggaggaatc agctcatatg atcgaaaaga taattttagg
     7681 aacctatacg cgtcgcgaaa gtaaaggaat ctatacgatc gaccttgata cagaaaaaga
     7741 agaattagcc aacctttcat tagctgttga agaaaatagc cctacctatg tagcaaaaag
     7801 taaagctggc aatctttaca cagttactag tgtggatgga ttaggtggtg ccggtgcgta
     7861 cgataaggat tttcattttt tgaatgcggt aacagagtct ggcgctcctt tgtgttatgt
     7921 agcggttgat gaagcacgtc aattactata cggggcgaac tatcataaag gtgaagtaaa
     7981 tgtctaccat atattaggag atggcgaact gaaagcagca gattcaatat tccatcaaga
     8041 agcgacaggt cctcataaaa accaagatca tgcgcatgtc cactatacgg atctgactcc
     8101 tgaccaacga ttagtcgtat gtgatcttgg tacagatcgt gtgtatactt atgatgtgtc
     8161 cgaaaacggc aaattgaatc ttgtaactac cttcactgca gagccaggaa caggccctcg
     8221 ccatcttgtc tttcatccaa atggacaatt cgcttattta tttggtgaat tagacagcac
     8281 agtccgcgtg ctttcttata ataaagaaac aggtgctttt gaagaattac agaaaatatc
     8341 gaccttgcca gaagaattcg acggtgaaaa tggaggggca gctatccgca tttcaaatga
     8401 tggcaagttc ctttatgctt ctaaccgagg acataactcg attgctgtat tcgcagtttt
     8461 tgaagatggt tcccacattc aaaacaagca aatcatctca acagaagggg attttccccg
     8521 cgatttcgca ctagatccaa gcaataattt tgtcgtgtgt gccaatcaga acaccgataa
     8581 tctgacatta tatcgccgtc atcctgaaac aggattactg gaattactga aaaaagatgt
     8641 ctatgtacca gaatgtgtct gtgtttattt tgatcgttag taagaaaagg agaatgaaat
     8701 gaatcagcaa gacgtattag acagactacg ggaagaacta aatattcctt tctttgatgg
     8761 gaaaatcgaa gataaagagt atagcgaaga ggaatatcag aaactcaaag ccgatttaca
     8821 gaactacttt gaacaatatg tacagaatgt agaaaactaa tcagctttcc atactatctg
     8881 aaaagaacat caggacaaga cttttccgtc tatcctgatg ttcttttcta caaataaaaa
     8941 accgccgaga tggttagact cgacggaaaa ggagtattta ctcgattttg agtaagtaca
     9001 aggttgttgt tggttatagg tatgtcatta tcataaagga ttttgatcac tctatcataa
     9061 aggtaatatt acatttatat tagataactc tagtttttat tgttatcaaa gatttctttt
     9121 attttttcaa caacaaatta gtcatttttt ggatggcagc tgtcaattgg tctgtcgctt
     9181 ggtcttgttt tgcttcagat gtattgaata gatcttcatc tacccctgat tcaagatcga
     9241 tactttggat catttcttca cagcctgcta catatgaaag ataagctttt tcaaaatttt
     9301 tatgaatgcc cattgctttt gctggtggac gcaaagtact gactttcata agcattgccc
     9361 gatatttttt tgttccttct tcaaataaca ggatgatctc ttcaagagtc gatttgtcca
     9421 gttcacttgt ttttccttca tcaattgcgt ttctgatcac ttcatatttc ggatgcattc
     9481 cttctccgat tgcttctgtt tccttgacga tttgatcgat ggactgcaca taatagccaa
     9541 tattcggtaa catgttcgtt ttcctacttt ctccattgtt gttattattt attctacttg
     9601 tttttatcct caaatgacaa gtacttgaaa aaatttaatg gtttctatac agctcgccat
     9661 tcttattcgt gctcaaagaa accatctgct aaactatatt gtttccctgc gacaatgtca
     9721 tattggatca attgatattc gtcgtaaatc tcttgttgcg agcgattgaa ttgcgcttct
     9781 ttataccact gtccgttttg atagtacagt tctcgttcaa acgccggaag ttcctgttct
     9841 aatgataaaa gcagctgata aaagccattt gttgtctgat tcgtctgttc cattagattc
     9901 gctgcaaaat aaaacggact ggtgattgca tcgtgagtcg tttgcttttc taattctcct
     9961 ttactgtcga acataagaaa ttgtgtttca tgcaatgttt gtttgtcatt ttttgcttgg
    10021 atcgtatctg aatagatccc tggaagatga tccccccaaa aaacaactaa agtccttcta
    10081 gaaagattct tcaattcttc cgtgaactgc ttcagtgccg tgctgctata ggaaatatct
    10141 tgcagataat tctctaagtc aagtgtccca cttccatctg aagtttttgc aggatagtct
    10201 agattatcat actttccatc gtagggcata tgtgtctgca tcgtgactaa atgaatgaac
    10261 tgaggtgttt tttcttcctt gagaagtgtc attacttctt tgtaagctga ctcatcagaa
    10321 atatatggat tattctctat cgtatccgta taggtcatcg tacgctcgct gatgaactgg
    10381 tcaaatccta atgtctgata aacatcctct cttttataca tcgaagtatt gtatggatgg
    10441 atcgctgtcg tctggtatga ttgtgcgttt aacgctgaaa cgatagaagg aagctgatcc
    10501 atttttggta caagcatcgt gtaaggggtg gtgagttgcg cattaaataa cgccatagaa
    10561 aaaccagtca atgcttcaaa ttcgatattt gctgttccac cgccatagtt ttgagaaagc
    10621 atattgccac tatatgtttg atcagctact tcataataat ctgccaatgg ctcaccagat
    10681 acttctacgc cgtttaatcg actaggatct gagaaacttt cactcatcac gaacacgatg
    10741 ttcggcgact cttcctctac agttttattt ttttcatcgg ctagcttctg gtatttttca
    10801 gtgatttctt tgatcttttc tttcgaatat ccttcaggtt catccattgg ttctaccttc
    10861 agattgtaca aaaacccacc gataaaacca gtgttatagt agttcatttt ctggctgtaa
    10921 ggtatccata gtgccgtttt attatatgct tttcgcaaca aattatcggg attgttgaaa
    10981 ttactgatat accctagcag tccaattgcc acaagcagtg tcgttaatcg gatcaattgg
    11041 aatcgccttc cttttttcag gcttcgaaac atgtaccagc aaaacaatcc tatcacggtt
    11101 cctgcggcag ctaagatcac tgtaaacagc atagtgccgg tcatatcttt cagcaatccg
    11161 atttcagtga tcatcttcaa atcgtctgga taaatcggtt cttgccgata actcattttc
    11221 atataatttg caaaacctaa tacaccgatg gctacgacat acgtaaggga acctaaaggg
    11281 acagatcctg ctagtgcaat cagaaaaata agaataatca gcaagacgag acaggctaag
    11341 aagaattttt ccgtatgcca agaaaaagca aatttcaacg caaggtctac ggatagatca
    11401 ttttgacacc actgcaaaaa taaattacta aaaacaacaa gaaaaaagat actgatgatt
    11461 gctgctgttc cttttaaaat ctttttctgt ttggtcgaaa gtctgcttat atatcttttc
    11521 aaatgcttgc tccttcattt ctatcaataa aagaatgttg cttctcttat tttagttgta
    11581 cgaaattcga aagtaaagaa cggaaagaag aattaatgaa atccaaatat tctctttctt
    11641 tatggccttt ctggtttctt taataagcgg acctttcgta ctccagaaat tttttctagc
    11701 tgttgtttca cttgattgag ctgttcaaaa tcagtactgt ctatgtcaat caatatgtag
    11761 acatgctcat tcttatggtt acgcgatata tctgcgatcg ctagctcgtt ctcattcagc
    11821 agagaaaata tctctgctaa tatattggtt ttcttttgat aaaatatagt gaatcgaaac
    11881 ggcgcttgga aaaacattcg tgcggatgga taattgaccg cttctctaac cgtaccgaac
    11941 aaaagataat cttttaaggc ttcaactgcc aaacgtccgc tgtctgccaa agcttcctga
    12001 gtcgatcccc tagatgaggc aacataagta tacggtcatt ttctaagaac tcttcttctg
    12061 gaaaatcagt caagtattta gccagatgtt gtttttccaa tgcttgcata acagcatatt
    12121 tatcaacaat ttcacttctt cctacattga taagtacagc atcttttttc attttttcta
    12181 tattcgcctg atcgatcaat ccttttgtat cttctgtcaa cggcagcatg actacgatgt
    12241 aatccgaggt agaaagaact gtttctaaat ctgcttgccg aacataatcc aactgggcat
    12301 cgcggataga ataacccaag acatccatcc ctaagctata gcaacttagc gctacttttg
    12361 tgcctatcgc tccaagacca agaagaccga tggttttgcc ttgcaattct cgtcccacat
    12421 aagcacttct tttgttttct gcctgttcta aaatattagg acctgttagt gtttgtacca
    12481 tacgacttgc ttcgattatc ggacgggaag ataagagcaa acagcacaaa acaagctctt
    12541 ttactgcatt agcatttaca cctggtgtat tcatgacgat cgtcccgttt tcagaagctt
    12601 tttcaacgtt gatcgtgttc accccaacac cagctcttga aattgctagc aaatctggtg
    12661 taagccattc atctttcact ttcgtactac gaataactat tccttcggct ggcaaatcat
    12721 ctattatttt gaaatattcc ccttgtaact tttctatctc ttgttctgga atagcatcaa
    12781 tcaaatgaac gtgatacata aaaccccctc cttactaaca agtatctcga aatttctgca
    12841 attagacaac tgtttaccat ttgacttcct agaagcaaaa agagcagtta gagcagagta
    12901 ggctgagtga taaaaataag gctatgacaa gttactgcca taagccttat tttcgagagc
    12961 aaaatattga gggtgagata tcttgataaa tggtgttccg ttagctgaca ttgataataa
    13021 gactaaattt caaaaaagtt gaaaagcaaa tttcgtcaat ctcttcttat ttcttaaaac
    13081 taagtggctt tttcacaacc tctttttatc taataaccac tgtttccaac tcaactaatt
    13141 ttgcccacgt ttcaatttga tctgttgtta gatttagcga gacaacggta tgatgaccgc
    13201 cacctgcctg aatccatgaa tggacacctt catggaaatt cggtttagtt ttccataaca
    13261 cacgtgcaac aggtaaatta ggtgcttcag tcgttggttc aaaggcttct acttcattga
    13321 tcagcagttt ataatgcgta ccgaaatctg ccatcgatac gactacccct tctcctgctt
    13381 tcccatcaaa tacaagacgc gccggatctt ctctatctcc cattgataaa ggagaaacaa
    13441 cgattcttgg tttcgtcgca gcaagcgtcg gatctacttc catcatatgg gattccaaga
    13501 ttgcttcttg gccactagct agctcgtaag tataatcttc catgaatcct gtatctttcc
    13561 ctcgagccat gattttcagc aaccgatcaa tcgctgcagt tttccagtcg ccttccccag
    13621 caaaaccgta tccttctgcc atcaaacgtt gtacagctag tccaggtagt tgtttcattc
    13681 catacaggtc ttcaaaatta gtcgtaaaag cgttatagcc tccttcttct aagaaacggc
    13741 gaataccgat ttcctgctga gcttgtactt ttacatgttc ctcccatgtc ttttcttcat
    13801 aatcaccata gtcgaaggta tacaattcct tgtactcttc aaataattga tcgacttctt
    13861 cgtctgatac gcgatcgatt acttgtacaa gatctccgat accaaaataa tcaacagtcc
    13921 aaccaaattg aatctgtgct tccactttgt ctccttctgt gacacccacg tttctcatgt
    13981 tgtcaccaaa acgagccact tttattccaa aactttcatt ataggctact gcaacatcca
    14041 tccaatcagc aatctctttt tggatctcag gtcgtttcca gtagccaacg acgattttat
    14101 tttgtttatt taatcgagca ttgatgaacc catattcgcg gtcaccatga gcggattgat
    14161 ttaaattcat gaaatccata tcgatcgttt tccaaggaat gctttcgtta tattgcgtag
    14221 ctaaatgaag caatggtttt tgtagaagct ttgtcccgcg gatccacatt tttgctggtg
    14281 agaaagtatg catccaagtg atcacacctg ctacttcttc tcgataattg acctctttca
    14341 tgatttttgt gatcgtatca gcagtaactg ctaattcttg aaggcggatt ggataaggca
    14401 attggccatt ttcattcagt tcatcgacca tttcttgtgc atgtttcttt acttctcgca
    14461 gtgcttcttc cccatataaa tgctgtgatc ctacaacaaa ccaaaattct ttttctccaa
    14521 tattcaacat aattagccac tccttttatt tgttttgtcc gtaatacgca ttttttccgt
    14581 gttttcgtaa ataatgttta tctaaaatcc gttgaggcag ctcttctgaa aatgaattga
    14641 gctgtctggt aaataaattc atcttgcata cttcatctag tacgactgcg ttcattaccg
    14701 cactttgcgc atctttcccc caagtaaatg gaccgtgtcc gtgtaaaagt acccctggaa
    14761 ttgctaaggg atcaattttg cgttctttga atgtttctat gataactttt cctgtttcat
    14821 attcgtaccc gctgtcgatt tcctgttgtg tcaaaaatcg agcacaaggt accgatccat
    14881 aaaaagtatc tgcatgcgtc gtccccatcg caggaacatc caatcctgct tgtgcccaga
    14941 tcgtcgccca tgtcgagtga gtatggacga ttcctccgat ttgaggaaat gaacgatata
    15001 aaacagcatg agtaggcgta tccgaagagg gattgagttt accttcaatg atatgattgt
    15061 ccaagtccac aacgaccatg tcgtctgctg tcatctcttc ataactgact ccgctaggtt
    15121 tgataacgaa atatcgtgtt tcaggatcaa aagcgctcac atttccccat gtatatttga
    15181 cgagtccgtg tttaggcaag tcaagatttg cttggtatac ttcttctttc agttgttcca
    15241 gcattttcta tcactccttt ttcttgcaat tgatgaatcg ctgttttttc aatgtcgatc
    15301 ccctcacggt aacgtctcat aaatatctga tagcctgcta tttcttctgc tgaaggtgtg
    15361 atggtgattc cttggtcttg tccaaacacg tcttctgcca agaagttttc taaagacttc
    15421 ccatgtcgat ccatcatata ggctgctaat aacgcgattc cccacgctcc accttctccg
    15481 gctgtgtcca tcactgtaac tggtgcctcc atagcagatg ctagaatccg ctgtccaact
    15541 tctggtgttt taaaaatccc cccatgcccc actaaacgat caatttggac attctcagat
    15601 ttcaatatat ccattccgat tctcattgca ccaaaagcac tggctaaatg gatccgcatg
    15661 aaattcgcta aatcaaaacg gctacctggt gtacgtacaa ataatggcct tccttcattt
    15721 actcctgtaa tgttttcacc ggaaaaataa ccataagaaa gtaatccccc gcaatccgta
    15781 tcgccttcta gcgctttgtt gaataatacc gtgaataatt tatctctgct tatctccata
    15841 cccaaagaat tggtaaattc ttcaaaaata ctgacccaag cattgatatc cgaagagcag
    15901 ttattcgtat gaaccatgcc tactagttct cctgaaggtg tagtgaccat gtcgatttcc
    15961 ggatggacat tttctagttc tttttctaat acgatcatag cgaaggcgga agtaccagcc
    16021 gaaatgttcc ctgtcccttt ggctacactg ttcgtagcga ccataccggt tcctgcatcc
    16081 ccttctggcg gacaaaccgg gattcctggt ctcaaatttc ccgtagggtc gatcagataa
    16141 gcccctgatt cgcttaactt gccagcttct tctcctgcta ctagaacttt aggcaaaatg
    16201 tgatccaagt caatcgctac gtgcgctttt tcgattaact gaccaaaaat atttttcatt
    16261 tcttgatggt aggttttcgt tgaaggatcg ataggaaaca tgcctgatgc atctcctacg
    16321 cctagtactt tctcaccagt aagctgccaa tggacataac ctgcaagcgt tgtcaaaaaa
    16381 ttaatttctt tcacatgttc ttctttattc aaaatagatt gatacaaatg agcgatactc
    16441 catctctgcg gaatattgta ttgaaatttt tccgttaata ttcgttctgc ttcttcagtg
    16501 attgtatttc gccaagtacg aaatggtact agtaactgtt ccttgctatc aaatgctaga
    16561 taaccgtgca tcattgcact gaacccaatc gacccgattg tcgtaagatt ctcgccatag
    16621 atttcgaata cttctgctgc catttttcgg taactgacgc gcagtccttt ccaaatatct
    16681 tctaatgaat aggtccagat accattttct agtttgtttt cccactcata actaccagcg
    16741 gcaataggag taaaagatga atcaatcaat acagctttga tcctagttga tccaaactca
    16801 atccctaaag atgtacgacc atctatgaca tctaacatac tttgcgcatt tacttccttc
    16861 ataatgattt aactccctcc ttctcgtcaa acctgatcaa accgtctttt tagtagcgct
    16921 tacaatgatt attgtatact tttgtacgta catgtcaagc agttatttac aagaatacaa
    16981 taaaaaacac ttgttttgta cgtacataag ttataaaatg aaattgctta caaaatctga
    17041 tcaaccgcct tctcgaaatt ctagaaggag agaatactta tgaaaaaaga aaaaaaagtt
    17101 tcctcatcgt ttatttattt ttttggcgca tttggcggca ttctatttgg atatgatatt
    17161 ggtgttatga ccggcgcctt acctttttta cagcatgatt ggggtttggc aggtaaagct
    17221 tctttgatag gatggatcac gtcttcggtc atgttaggtg cgatccttgg cggctcttta
    17281 tctggtcttc tttctgataa gcttggacga cgaaaaatga ttcttctttc tgcacttatt
    17341 tttatggctg gttctgtcct ttctgcatct gcccctcaca atggttctta ttttctgatt
    17401 gcagcaagga ttcttctagg tttagctgtt ggcgctgcat ctgccctagt tcctgcttat
    17461 atgtctgaaa tggctcctgc tagactaagg ggaagattgt cagggatcaa tcaagtgatg
    17521 attgcttctg gcatgctcct ttcctatgtt gcagattatt tgctaaaagg gttgcctgaa
    17581 acgatggctt ggcgcgtgat gttagggctc gctgctgtcc ccgcgctgat tttatttttc
    17641 ggtgttttag ctctaccaga gtcaccgcgt tttcttatgc aatctggccg cttggaagag
    17701 gcaaaaagag tattgaacta tattcggaca ccaaaagaag ctgaacagga attcgaacaa
    17761 atccagctga atgtcaaaca agaaaagaca accggaactt cttggcatac tttgttttta
    17821 gaaaaatatc gttccttagt cattgccggt attggtgtag cagtattcca gcaatttcaa
    17881 ggagctaatg ctatttttta ctatattccg ctgatcgtag aaaaagcgac tgggcatgca
    17941 gcgagcgatg cattgatgtg gccgatcatc caaggaatca ttttagttgc tggttctttg
    18001 ctctttttgg ttattgcaga caagttcaat cggcgaactt tattgaaaat aggcggtagc
    18061 gtgatgggac tttctttcat cctaccagct gttttaggaa ctgtactgga tgctcataca
    18121 aattcgttgt tgattctctt gtttttatgt atttatgtcg cattttattc ttgcacatgg
    18181 gctccactga catgggtaat cgtaggcgaa atattcccac tagctgtccg cgggcgagca
    18241 tctggcctag cctcttcttt taattggatt ggttctttcc tagttggctt gctttttcct
    18301 gtgatgactg cttctatgtc tcaagaaatc gttttcggca tttttgggat catctgcttt
    18361 ttaggggttt tatttattca agagatcgta ccagaaactc gtgggaaatc tttagaggaa
    18421 atcgaacaaa gtgcatctaa aaaaacttat cctaaacgta tttctatcca tcattcttaa
    18481 agaggttgtg acagaagtgg tcagcttcaa gaaataagaa ggaaccgctt agtttccagt
    18541 cacaaggaac aatttcaaaa aacagcttct catgtttgat gaatattgtg acttgtgacc
    18601 agttggtagg agaacaccgt ttagtggaga ggtggtgaaa gaaacgttca gccttgagcg
    18661 atgacaaaag ctctcacaaa cattgcgacg agtaaccgta ggagcaataa gaggcggtaa
    18721 aaacccgtct cttatttttc gttttctttc actgtttccc gttcaactag tacaggttca
    18781 tagagaatat ttggctgtgc ctgtcctttt tcaatcgttg tgacgatcca ccgtgcggca
    18841 tcttgtccca acttttcttt tggatgcgtc aatgttgtca attttttatt ccctaaatgg
    18901 cttaaagaag aatcgtctgt tcctacgatc gataggtcat ctggaatatg atagccattt
    18961 tctgtcaact tatctagcat taaactagct atttgatcgt tatagcaaat gatgccatct
    19021 aaagcttgtt cttctttcaa ccgttgtact gttttttcag cgattgtttc tcgtgtttcc
    19081 gtggtgaaag taatgatatc ttccgtcaaa aaacgcacat tatactgttc acacgcgctg
    19141 ataaaacctt tcatcctgta ttttccttga agatcgtcga tttttgtgat gaacaacaga
    19201 cgctgatgtc ctttttttat caaatattct gctgccataa aaccgacttt cacatcatct
    19261 aaacaaatat aagaggcatt taactcttca taagcagcat tgatcatcac catcggaatg
    19321 ccttgttctc tcaaagcaac ataaagtgct aagtttgggt tatactgatt acttttcgta
    19381 ggctcaacga tcaaaccatc taccccataa gcaatcattt tcttcaaaca ctctttctcc
    19441 aattcgtgat cgttattcgt actggctaaa agcaatgaat acccagattc tcgtaattca
    19501 ttttcgattc ctcggataat cgacggaaaa atataatcag agagatatgt ggtgatgaca
    19561 ccgatcgttt ttgttctgtt caatggtgtt ttttctttct tatagctgtc atctacatat
    19621 gtgcccgacc ctttttcttt acgcagatag ccctcattta ccaacacagc gattgcttgc
    19681 cgaatcgtat gtctgctgac gtcatatatt tcttgcagct tgaattctga gggaatggtt
    19741 gtatttactt cgtattcctc acgaaggatc ttttgtttta tctcttctgc aatatcttga
    19801 tattttgcca tttgccttgc ccctttttat ttgtacgtac atatatcata acgagtctgc
    19861 aaggtttatt caagcaaaaa tcaaggtttc ttgtgctcat aaggcatacg gaacaggttc
    19921 accaaattct ggaaatcctt gctcattcca cgtaaagcat tgcgcgttcg cgtgccgatt
    19981 aggattgtct aacggatccc ctttttggtt cttctctggt cgagcatgat agatcaaaat
    20041 atcaaagcac ccgtcttctg aaaccgtaaa cgagttgtga ccaggaccaa attgcttatt
    20101 tttttcagaa gaaacgaata ctggctccga taatttatgc catgattctt ttaataaaag
    20161 attcttgtgc tcatcagccc acagcaggcc catggcatag ttttcatctg ttgcactacc
    20221 agaatacgta atgaatattt tcccattgcg agtaaggact gctggacctt catttactga
    20281 aaaaccccgt ttttcccatt catactcagg tatagaaagg aggatctgct tcccctttaa
    20341 cgtccaagga ttatccattt cggatagata aagattagaa ttcccagtaa tttcaggatc
    20401 ttgttgggcc cacacataat atagctttcc ttgagaagag aaagttgttc cgtctaaact
    20461 gaaagtttcc cttttcgttt ttatttgtcc cttttctatc caatctgtat tcattggatc
    20521 tatatgctga ttttcaagta cgaacatgcg gtgatgatga gattcatcac gtatagtttt
    20581 atgattgctt gcagcaaagt aaatatacca ttttccattg atataatgga cttcaggtgc
    20641 ccagatcaat tcactcatcg gtccttcttc atgagcctgc catacaatca ggcttgcagc
    20701 atgttctaat tcattcagtg actttgccct tcttaattca atcacttggt agcctggaac
    20761 cgatcctgta aaataataat aaccatctgt atgtttatat acccaaggat ctgcccgttg
    20821 ccgaacgata ggattattat aaagtgtctt catcataaca aatttcctca cattttcttt
    20881 attctaatta cattccaaga gtatttttct atccctacat ttaattcgtc atgtttttct
    20941 attgaaaaat agtttatttc aggttttatt ttttctggtt cttcacgtgt atttttgtcc
    21001 aacagatcgc tactggttaa tgtgtagtgc tctccttgat ctagcaaatt gaaatcatgt
    21061 tcaatcgtta tcttttgttg ttcatttgtg cgattgatca agaaaatagt cagctgattg
    21121 ccgttatcta cgatcacact gtccacataa ggaacatttt ttatcttttc acaatcataa
    21181 acagggcaaa tgatttgttg tttcaatact tgacctttag catatttcga taagtgttgc
    21241 atcacataat aagttggatt cctccatgct tgtcctttag catctgtcag tattaaaggt
    21301 attgtgttta ctaacaatga ttgacaacta atctttacgc gatctgcgtg tcgtaaaata
    21361 gccagcccta atgaaccaaa taataatgta tcttccatag taaagtaaca ccaatcgatg
    21421 ggactaccga cttcaaaatc cctatgtctt ttttccggtt gggcatgtac attccattca
    21481 tcaaacgcta gatacattgt tttctcactt ctcaaatatc cttttacata atcacaagta
    21541 gcaacgattt cttcaatttg acgatcgaat cgataagctt ttgcaaggta tgtcgctgta
    21601 tcatcacgct catttttagg aggtttagtc aagtcttctt catcatactt atcaatatag
    21661 ttatgcaaag ctaaataatc tacattctca taagcttctt ctaatgcgat acgatcccat
    21721 tcagggtacg atggtaaacg tggcgtagag cttccaacta agattgtttc aattgaatca
    21781 tccactaact tcatgacttt tgaggcttca tttgctaacc taccatattc ataagcactc
    21841 ttagccccca cttgccatgg accgtctagc tcattcccta aacaccataa tttaacgcca
    21901 aaaggctttt tttgcccatt ttttattctt aaatcactcc agaaggttcc ttttggaaaa
    21961 ttacaatatt ctactaaatt ttttgcttca tcaattcctt ttgttcctaa attcaccgtg
    22021 aatatcggtt tcgcttgtac atgactgatc catttaaaaa attcgtggag tccaaaagaa
    22081 tttggttcta tacctcgcca tgctaaatca attttttttg gtcgttgttc tttcctacct
    22141 acagtatcca cccaatcata accagatgta aagtttcctc caggatagcg aatgacacct
    22201 aattctaatt cctttacata agagaggaca tcttgtctaa acccgtcacc atcagctgtt
    22261 tcatggcccg gttcataaat ccctgtatag acgacacttc ccatatgctc aacaaatgag
    22321 ccaaacagcc gagaactgat ctctcctacc gtaaaatcct ctctgatttt tactttcact
    22381 tcttcgtcca acagctttcc ctacctttca tttcctatag tcaagataat agatacaagt
    22441 ttccatacgc caccagcaaa gataaaaata aaaggaggga aaagaaatat gagcagtaca
    22501 aatcctaata acaatacgca acaaagaata tttctcaaaa aatcaaaaaa tactttccat
    22561 gcactttgtt caaaaatttg taaaaatgtg atatctttgt tttcagaaat cacatatatc
    22621 caggataaaa gaaacatgga ataaataagt aaaactacat atccactaat tgaaagtacg
    22681 attgtctgtt cttctaataa ttgattaaca agaaaataaa taatgatcgt taacagacta
    22741 aggaacaagt tcttaccaaa atgttgcttt attccttgaa agaaaaaata aatgatattt
    22801 cctgatccct cacctttcca ttgcgccatt acataaaaaa cagcacatgt agcaggcacg
    22861 atagtaataa caggcaaaca cacgattacc cagagaatac ctaataagat gtaatcagaa
    22921 accacaatca actgtccata aagtttagtc tcaactattt tcatcttcct cactctttca
    22981 ttccagacat agctgttcct tgaacaaaat atttctgtgc aaaaatataa accactagta
    23041 atggcagcat tgaaataacc gttccagcca ttacaggtcc ataaaaagaa atgttctgcc
    23101 ctgtaaataa agctaaccct gctggtaaag ttctcatttt tgaactactt gtcaatatca
    23161 atggataaag caagtcattc caactattca tcagcgtgaa aatccctaat gataaaagag
    23221 taggtttgct caatggcaac atgattttca aaaaaatatt aaactcactt aatccatcaa
    23281 tgcgtgctgc ctcttccaaa gaatctggta aagtaataaa aaaggaacgc atcatgaaga
    23341 taccaaatgc agttgtcata cgaggaatga tcaaacctgc ataagtatca agcatgccaa
    23401 gcaaattgac ttcaataaat agaggaatca tgaagacttg gaatgggatc atcattgtca
    23461 gcaaaacgaa atagaacaaa actgattttc ctttgaaacg catacgagca aatgcatacc
    23521 ccgcaagaga atcaaagaat acagaagtga tcactgtccc tccagcaaaa atcactgtat
    23581 ttttgatgta atctaataaa gggatcgttg tccaaacctt aatatagtta tctagggtat
    23641 agctatctgc aaatattcta ggcggaaatg agataatgtc tttttcgtgt ttaaaagaag
    23701 aaataatcag ccacaaaaaa ggaaacgcaa taaccaacaa tataactagc atactaatcg
    23761 ttcgaatgat ttttttacca ctaactgctt ttcgactatt cacagtaact gtaatcgagc
    23821 tttctggtgc catacaggtt tccctcctat ttcatttgtt tttctttccg tgacatgtac
    23881 acattagtgg agatagctat agttgcaaca atgataaaca aaaccgaaga cagtgctgat
    23941 gcatagccca attcataagg tgctgtaaat cctcgactgt aaatatattg aacagctgtt
    24001 tctgtcttaa attgtgggcc accagcggtt gcaacatata cttggtcaaa cacttgcatc
    24061 gaaccaatca gattcgttaa aatacaaaaa cctaaggagg gaacgatctg aggtagcgta
    24121 atgttaaaaa actgctgtcg atttgttgca ccatccattt gagcagattc atacaaagag
    24181 ggagagattc cctgaatagc agtcaagaga atcgtcatag taacaccaaa attcttccaa
    24241 acagtcatga tggctaccgt cggcatagca agggttggat cgcgaaaaaa ttgagggatc
    24301 ggcaagccga cttgtgaaaa caagtaagga atcgccccta cattcggatc aagcaacata
    24361 gaaaaaataa tcgcaattga cgtcaatgaa caaataactg gaatgtaaaa tgtcgaacga
    24421 caaaaacgat tgaatctggt atttttagat aatgctactg ctgctaataa accaacgata
    24481 atttgtgttg gcgtttcgag tacggtgaaa tataaagaat gaatcaacga attaactgct
    24541 cgttcatcac taaatatacg tgtgaaattt tcaaaactta caaaatttct tgatgtaaag
    24601 aaaatattca aatctgtaaa actaatagca aatgttccta aaagcggtat gacagtaaat
    24661 aataataaaa ctaaaaaaga tggagttaaa aacaaatacg cagcacgatc aggacgattc
    24721 caatatttgg tgaatccgtt catatagtcc acctcatctt tcattttgat aaaatataca
    24781 atcttggttt aaacgaatct gaataagata tccgaacaat cgctgtgatt tttgcaataa
    24841 cacctatctg caaatactag gtcgatcatt cagatatctg tatcttgcca acagtctatc
    24901 ctgtataaag ttctgtacta tttttgcagt aattgattga tggcatcatc tgcatcgttc
    24961 atgagtgtct caggatcttc tcctgctaat aatttttcaa tcagtggatt gatgatatcg
    25021 ttattgatcg cagagatttg ggtaaaacct ggtaaatatg gctctgcata gtccatttgt
    25081 ttcgataatt cagatacaat aggatcattt tttacttctt catcttttgc tactgaatcc
    25141 aagtaggcag gaaaaccatt ttcgatactc cattttttac caatttcagt tgtattccaa
    25201 tatttcaaaa actcatagac agcttctttt ttattattat ctgtacttgc cggaatggca
    25261 tatcctacgc catctaaaat agctgttttt ttgtctgaat cactcccttg tggtaaggta
    25321 gtcacattat aatcaatctc gtttgctttc aatccgttat ttaaccatgg cccattgatt
    25381 tcaatggcta gttgaccagc attcattaaa ttgtccattt cggcgccaga aatcgattct
    25441 ggaccagtct tgtctacatg gatcattttt tggatcgctt ttaacgtttt aaggttctca
    25501 ggagacgcaa attcagactt agtataatca tcgttcacta atttaccgcc atttgctaac
    25561 atccagttat ataacggtgc agttccatcc ttattgaaga caaaacctga tacattttta
    25621 ctactatcag ttaattttgg tgccatttct gtcaattctt cccaagtagt aggtggttgt
    25681 tcagtatcta atccagcttg tttaaacaaa tcattattcc aatacatata catgccttgt
    25741 acctgcatgg ggacaaaata ctgtttacct tctacttcac ctaattcaat agccgtttct
    25801 ttaaaatcag atttatctac tccatcatat tcccagaaat cgtctaatgg ttgtaccgct
    25861 ccgttttcta catactgtgc aaaatctgga taatttaatg caataaagtc aggtgctttt
    25921 ttagaagaaa tcgctgtagg aagtttttca ttcaaattgg cccaagtcat gatatccatt
    25981 tcaattttga tgtttttatc atttgtttta ttaaattcat taacgatttc ttgcaatatt
    26041 tcaccatcag atgcagtaaa cccattccaa aaagtcaaag tcacttcttt atcagaatca
    26101 gattctgctg aacttccgcc accgcaagct cctaaaatac ctgttgcagc taccaacaaa
    26161 gagccaatta accatttttt acctttcatt ttttgttcct ccttcatctg ataaacgaca
    26221 tctgaccaca ttccaagata gcggtttcaa taaacaattg attttcccat cttcagcttt
    26281 tacatttgtt gaatgacttg gttttacttg ttcactttta ggaagattag ttgcttttgt
    26341 atcaaatcca ctcatttcag aaaaatcaat cactccctct aatgataatt cagacaagtg
    26401 aacagttaga ttcatttctt cttctccacg gttaactgca aatatgacaa gttctttgtc
    26461 atttattacg gctatacttt ctaaatacgg cacttcttca aaatcttccg tcgcatagtt
    26521 tgctgaacga atttgtgggc ttaacacctc tccttgtcca tacacagcag cttgcataaa
    26581 tggataatag atcgtctgtc tccaagcttc tccgtttgtt tctgtcctga ttggtgcaat
    26641 aacatttacc aattgagcaa gacaagcaat ctttactcta tctgcatgtt tcaataaagt
    26701 gattaataga cagcctaata gtaatgcatc ttcaaaatta tagttatctt ctagtaaagg
    26761 aggagcaatc tgccaaggcg ctgcttgctc atcttgttcc gctgaatgat accaaatatt
    26821 ccattcatca aaggacaggt tgatctgctt cttactatgt ttcttcgcct tgactgtatc
    26881 gcacatcgct gtcacttcag aaatgaaccg atccatgtct aaggaacgtg caagataatg
    26941 agaaagatca tttttttggt tgccataata ttgatgcaga gacaaataat ctatttgatc
    27001 gtacgcttga tcaagaactg ttaactccca atcaccaaat gtaggcattt gccgattcga
    27061 acttccgcat aaaaccacct caattgaatc atctacgagt ttcatcgcct tagccgtttc
    27121 ctctgctaac cgtccatatt cttccgctgt tttatgtcca atctgccaag gaccgtccat
    27181 ttcattccct aaacaccatg tcttaattcc aaaagggctc tcttgaccat tcgaacgcct
    27241 taaatcactc caataagttc ctttaggaaa attacaatat tcgactaaat tacgagctgc
    27301 atctactcca cgagtaccca gattgacagc catattgatt tcggccccca catgctgaca
    27361 ccagttataa aattcatgaa tcccaacttc attcgtttca attgtccgcc aagctaaatc
    27421 caaccttcta ggtctatttg ctatgggccc aatactgtct tcccaacgaa atcctgacac
    27481 aaaatttcca ccaggatatc taatcatggg aatgttcaat tcttttacca agtgaatgac
    27541 gtcttggcga aatccacttt tattagctga tggatgatct ggctgataaa tcccttcata
    27601 tactgctcgt cccatatgtt caataaatga actgtacaac cgcggatcaa ctttgcctac
    27661 acgcttgaca ctactaatca acattgtggc ttccataact gacctccttt aatttataca
    27721 tgaataatac acctcgttga aagcgttgtc aataatataa atgttaaact tacttatttt
    27781 ttatcgattt taattaacca aaatattaat aatgattaaa acaaataatt taatataact
    27841 tttattaaca aatatgttaa tatataagaa aaacctttta agaggtgata tatatgcaac
    27901 tacaaataaa cgaagaatcc ttaccggtat atgaagcatt agctagtaaa acaaggatca
    27961 agatcatcca gctactttcc aaaaaaaaga tgaatgtcaa agatttggca aaagaacttg
    28021 gagtgagcag tgctatcacg acgatgcacg tgaaaaagct agaggaagca aacatcatca
    28081 aaacagaaaa agtaggacaa caaaaaattt cgagccttcg tgtcgataaa atcgatatca
    28141 gttttccaga aaaaatattc aatgcttttg atacgaaaga aacatcgata cctattggtc
    28201 actatacaaa ttatgcaatt gaaccaacat gcggtttagc tacgatccat gattttatcg
    28261 ggaaagtgga tgaaccacgc tattttatgg atcctcgaag gatggatgcc cgcattctct
    28321 ggtttaccag cggatttgtc gagtatcagg cacctaattt tttaaataca gaggatacat
    28381 tggagatgtt ggaagtttct gtggaaattt cttctgaatt tcctttttca aatgacaatt
    28441 ggccttcaga tatcactttc agcctaaatg gtgtggagct aggaacatgg acgagccctg
    28501 gagattttgc tgatatccgc ggtaaatata cgcctgattg gtatccagat aatcttaatc
    28561 aatacggctt gctgaagacg atccgaatta ccaaacactt gacgaatatg aatggagaac
    28621 ccttgtcaaa catcacgatc aacgacatcc ctaaagagca agacacttgg catttgcgaa
    28681 tcgaagtgaa agacgatgcc aaacatgtgg ggggctgtac tttatttggt aaaggattcg
    28741 gtaattacga tcaggatatc aaattaaaag tttactacag ctgatatttt tcttttctat
    28801 tttataaaac aaaaacagcc aatccctatg aagggacggc tgtttttgct ttattgttat
    28861 ttccagaaat catccaaaac ggaaattggc aaatgtcgtt tatgttgtgt cttgttccac
    28921 catgcttcga ttttttcttg tgcttgagcg ggaacttctt ttccttctag ataatcatca
    28981 atttcattat atgtgacacc tagtgcgact tcatctgcaa tcattggttt cccatcttca
    29041 agatctgcag tcggaatttt tgtgtataat ttttccggtg cattcaatgc ttgaagcaat
    29101 tgtttccctt gtcgtttgtc caaacggaat aatggcaaaa tgtcggctcc accatcacca
    29161 aatttcgtga agaatccagt aatgttttct gcagcatgat ctgtccctaa tacggctcct
    29221 gctctttctc cagctacggc atattgcgta atcatacgtt gacgtgcttt gatatttcct
    29281 ttgttaaaat cgctgattcc cacacctgct tcgcttaata cttgtacttg tgcatcaaca
    29341 gctggtttga tatttactcg cagactaaca tcgggctgga taaagtcaag agcagctttt
    29401 gcatcttctt catctgcttg ttcaccataa ggaagacgaa cagcgataaa ttgatagttt
    29461 tcatcttttg tttcttcacg catttcttcc atcgttagtt gcgccaaacg gcctgctaaa
    29521 gtagaatctt ggccgccgct gatacctagt acaaaagttt taagaaacgg atattttttc
    29581 aaatatgctt tcataaagtc aatactttta cgtatttctt cttttggatc aattgttggt
    29641 ttgacaccta attcattgat aatttctgtc tgcaggctca tttattctcc tcctatgctt
    29701 ctgtatgaag ttctgctacg ctttttttca ttcgttccat gatagccatt ttatggttcc
    29761 agcattcagt agacagatcg actgggtatt tttgcggatt caagtctcgt ttatactctt
    29821 cccatagtga atctagattt tcttttgtat attctttgat ttcgtttagt gcaggtaatt
    29881 cgtaaactct ttttccttcg ataaagatat cttgtaaaac tgggcgagct gtaaagtctc
    29941 gaacagtttt attgataaac gtatgcactg gatggaacat gaagatagct tcttcttctc
    30001 gagggtcttc atcccataat gtcacgtagt ctccttcaga tttcccatca aaatttcgag
    30061 tgatgcgcca cacttgtttt ttccctggag tagttacttt ttctgcattg cttgataact
    30121 tgatcgtatc catcattttt ccttcatcat cttcgatcga tactagcttg aatactgcac
    30181 caagggctgg ctgatcatag gctgtgatca attttgtccc aacaccccat acatcaattt
    30241 tagctttttg catctttaaa ttcaaaatcg ttgcttcatc caagtcattc gaagcataga
    30301 tttttgcttc cgtaaatccg gctgcatcta attgttcccg aacacgctta gatatatagg
    30361 ccatatctcc actgtcgatg cgtacgcctt ggaagttgat tttgtcacct aattctttcg
    30421 ccacgcgaat cgcacttggc acacctgatt tcaacgtatc ataagtgtcg actaagaaca
    30481 cgcagtcttt atgggtttta gcgtaggcca taaatgcttc ataatcattt ccataagatt
    30541 ggactaacga atgagcatgg gtcccgctag ctggtatccc aaatatcttc ccagcacgta
    30601 cattgcttgt tgcatctgct ccgccgatat atgccgctct agttccccag actgctgcat
    30661 ccagttcttg tgcccgtcta gttccaaatt ccaatagtgg atcttctccg atgacagatt
    30721 tgatacgagc tgcttttgtc gcgatcaacg tttggaaatt gaccatattc aacagtgctg
    30781 tttctactaa ttggcagtgc gctaaaggcc cttccacttg gattaatggt tcatttgcaa
    30841 aaactagttc tccttcacga gcagaacgaa ctgttgcttt aaattcaaaa ttttgtaaat
    30901 actccaaaaa accttcagga tagacttcta atccacgcaa gtaagcaata tcggaatcat
    30961 tgaacgtcag attctctaaa taattgacta accgttccaa tcctgcaaaa accgcgtatc
    31021 cgtggttaaa aggcatttct cgaaagtaac attcaaaaac agcatgtaaa tcagctcgac
    31081 ctaattccca gtatgtctgc atcatattga tttgatatag atccgtatgc agcgtcaaac
    31141 tgtcgtctgg gtaagtatta ttcatcttgt attactcctc ttggcaagtt ttcatttcct
    31201 acccattata acaaaaaaaa ttaataggaa aagaaatctt ccattttttc tgaaaaagca
    31261 catgttttac ggatatttct tgctaaaaat gaactccatc tgctaaaact atcctttctt
    31321 attggtattt aacctaaacg agaaagaata cttattttat tcaatgtatc cgagacacga
    31381 agaaattagc gttttctatt tttcttactt gataaaataa gtagattttc ctactacaat
    31441 cgtagccgtt aagataccga tgattgaaca aatataacca aatgtctcta caacataatc
    31501 aatggcaaaa gtttttgaag caataatacg tctttccttt tttgcttctt atccccaaag
    31561 aaacggatat aacttattat gtattgaatt tttttggaaa ggcgaattat gacaagatat
    31621 accttgcgtt gaaaaaactc gcggaaaaaa tatcacacgt ttttaaaaag atacaaagta
    31681 agaaagcagc tgtcaataga atggaaacaa aaatggcgag acatcaatca gtctcgcctt
    31741 gttttttatt gtatacatta gatatggagc aggttaccat aaagatgatt attagtcgtt
    31801 attaaataga atccttcgta tttttttgtt ctatcactac aatatcttta tttggacgtt
    31861 tgaattgtat tccggcacgt tctagctcct gtttcactcc tacatggact ttgacatctt
    31921 ctcgtgtcat ttttaaaacg tgatgaatcg catagtcgat ttcttgcctt gtgatttttt
    31981 tcttcgtatt gatcatcacg acttgctttt tatcatatgt gtctaccaat agcagttgtg
    32041 ttcgatctaa cgaaaataat ttgtaataat taatagccgt attcccaaaa aagaattcca
    32101 ctactttagg ataaaatgtc ttaatattta aattactatt cacaggtgtt tcgattagct
    32161 tcatctcatt cctcttttct atatagtcga tttagatcca atcttaagtt atgttaccat
    32221 ctttcctagc attaaggaat cggttacaat tgaaccttta aagacaaaag gatttacctt
    32281 ttatcactta atagtataac ataatttcgt gagaaaaaaa tacaattttt tcttgaccat
    32341 ctcgttacgt aaaggattat gcttacattt gtcaggagga aaaataatgg agtacacaat
    32401 aaagaaaatg tcagagatat caggagtcag tccgagaacc ttgcgctttt atgatgagat
    32461 tggactgcta aaaccagcta ggatcaattc ttcaggttat cggatttatg gcaaaaaaga
    32521 agtcgatcgt ctgcaacaca tcttatttta tcgaacaatg gcattcaagt tggatgatat
    32581 ccaagaggtc ttagacaatc catcttttga ccaccagaaa gctttaatca agcatcagca
    32641 aatgttgctg gaaaaacggg cacaaatcga taccctcttg acaactgtcc aacagacact
    32701 tgatatgtac gaaggaggaa gaaaaatgag tgacaaagaa aaatttgaag ggtttaaaca
    32761 acaaaagttg aaagaaaatg aagaatccta tggacaagag attcgacaaa aatacggaaa
    32821 aaaaaaggtt acagcatcca atgaaaaatg gatgaatatg gataaagaaa cgtatgaaga
    32881 aatgcaaact gttgaaaagc agatgttatt tgatctcact ctttacttaa aagaaccaga
    32941 agatgaaagc ttagcagacc gtatcttcca agctcataag aagtggttct cttacagctg
    33001 gcctgaatat cagccaaaag cgcataaagg actaggaatg atgtacgaag ccgatgaacg
    33061 gttcactgca tattatgacg agcgaagtgg tcatggtgca gcaaaagctt taaatgaaat
    33121 tatccaaaaa aaggcgtaat cttttacatg atccaacaaa aaagtacacg taaaatgacc
    33181 taataacaaa acagcagaca cccaagttag atttttatgc taacttttgg tgtctgctcc
    33241 attctttcac aagttttttc atcctacttt aaatatattc atcgttgaat aaacgtataa
    33301 ggtattttca ctttatcctt tttttctgta agaaacaatt gaaatgccat ttctccgcaa
    33361 gcttccagat ggtggtcgat ggttgggaaa ccaagaagct cgctgatcag caaattatcg
    33421 cgtccaatca ccactggtgt cttatcttgc agatagcttt gcaagatgcc tgctgcaacc
    33481 atatccgaat ttgtaaagac accatcaact ccaagctctt taaaaaagaa gcctgcttgt
    33541 tttcctttct ctgcatctat acaatcccaa aaaacaagtg attcatcaaa agatggaaaa
    33601 agttgatgac atagctgcaa agtgattttt gagttataac ttagcctgcc acttcgcccc
    33661 aacgtgatgc ccaattgctt tataccttgc tgcttcaaat aagtaagacc ttctgtaatt
    33721 gattgttctc gatcaataaa gacacagctt gcctgttttc ccttgatttc ttcacaaaaa
    33781 actagaggtc catatttttg atatgccaat aaatcatcta gtgtattggc acgagacgta
    33841 atgatcaatc catcgaaagc tttagctgca aattcttcaa gataccttcg ctctttttct
    33901 ttatgatagc ccgtcggtaa aagtgtcact ttgtaatcat aatcaaacgc tgcttcaatg
    33961 atcccactga tcaactgatc aaaatacgga tgattggtat aaggcagaat cactccaatg
    34021 cttttcgttt ttccataact taactgtctg gcatttgcat tagggatata atcgacttct
    34081 ttcatgaccg caagtatttc tttccttttt tcctcatcta catatggatg tccattgatt
    34141 acacgtgaaa tagtagaaac tgaataacct gtgagctttg cgatatctcg aatgtttgcc
    34201 actgctgctt cgccacctat acaaaaagat tttcttgtaa atagtctacc acagctggac
    34261 cgcattcaaa aaactgatac atttttcctt catgcgaaaa actgatccgc tgcccgcccc
    34321 aatatatatc ttttgtcaaa ttttcgattt cattcattgg tatttctttt actatattcc
    34381 tattcaaccc ttgaaaactg tacaacgtta atacggatcc cttgaactcg acaaacgcat
    34441 aaaaataatt gttcgttgtg ccatctgtta tctctacata agtattcatt gcaagccctc
    34501 cttttgatac tttgagtata gtgtatgaaa cgcgtctcat agcaaggagt ttgttttgcc
    34561 aaagaaaagt tttattcctc tttcattatt accaaagtct cttattttaa attgaataca
    34621 ttataatgat actgtaaata tatctttatt attagtgaat
//
LOCUS       DAMEET010000022        34636 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.022, whole genome shotgun sequence.
ACCESSION   DAMEET010000022 DAMEET010000000
VERSION     DAMEET010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 34636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.022"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            591..1280
                     /locus_tag="PB780_000903"
     CDS             591..1280
                     /locus_tag="PB780_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDE0732973.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="PB780_000904"
     CDS             1267..2475
                     /locus_tag="PB780_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDE0732974.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="PB780_000905"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="PB780_000905"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDE0732975.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="PB780_000906"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="PB780_000906"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDE0732976.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="PB780_000907"
     CDS             complement(6578..7273)
                     /locus_tag="PB780_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HDE0732977.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="PB780_000908"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="PB780_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDE0732978.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="PB780_000909"
     CDS             complement(8472..8663)
                     /locus_tag="PB780_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HDE0732979.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="PB780_000910"
     CDS             complement(8684..9574)
                     /locus_tag="PB780_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDE0732980.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="PB780_000911"
     CDS             complement(9561..10328)
                     /locus_tag="PB780_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDE0732981.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="PB780_000912"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="PB780_000912"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDE0732982.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="PB780_000913"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="PB780_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDE0732983.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="PB780_000914"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="PB780_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDE0732984.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="PB780_000915"
     CDS             14899..15282
                     /locus_tag="PB780_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0732985.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="PB780_000916"
     CDS             complement(15450..16208)
                     /locus_tag="PB780_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HDE0732986.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="PB780_000917"
     CDS             complement(16226..17044)
                     /locus_tag="PB780_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDE0732987.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="PB780_000918"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="PB780_000918"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDE0732988.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="PB780_000919"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="PB780_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDE0732989.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="PB780_000920"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="PB780_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDE0732990.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="PB780_000921"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="PB780_000921"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDE0732991.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="PB780_000922"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="PB780_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HDE0732992.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="PB780_000923"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="PB780_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDE0732993.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="PB780_000924"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="PB780_000924"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDE0732994.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="PB780_000925"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="PB780_000925"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDE0732995.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /locus_tag="PB780_000926"
     CDS             27086..27385
                     /locus_tag="PB780_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDE0732996.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="PB780_000927"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="PB780_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDE0732997.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /locus_tag="PB780_000928"
     CDS             27973..28209
                     /locus_tag="PB780_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDE0732998.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="PB780_000929"
     CDS             28352..30325
                     /locus_tag="PB780_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HDE0732999.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="PB780_000930"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="PB780_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDE0733000.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="PB780_000931"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="PB780_000931"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDE0733001.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="PB780_000932"
     CDS             33204..34496
                     /locus_tag="PB780_000932"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDE0733002.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10554 a         6733 c         6844 g        10505 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagac caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgttaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggattaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggcggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccgtc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagactat gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atgggaaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatct
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaattct tgaaaactat
    25441 atcgaacatc agcgcgaagt catcacaaga cggacagtct ttgaaaaaga aaaagcagaa
    25501 gcaagggcac acattttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaagcaaaag cgacgatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgcacagc caaaggaata ccaattatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggttagagg aatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aagatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaatag ccatcaagac taattgtgta agctagcaag
    26881 aatgaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataatcag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattggatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaac gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atatcgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaacact gtttattcag aataagggac tgtgacaaaa accgtgtcac
    34621 agtccctttt tccgaa
//
LOCUS       DAMEET010000023        34112 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.023, whole genome shotgun sequence.
ACCESSION   DAMEET010000023 DAMEET010000000
VERSION     DAMEET010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 34112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.023"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1152
                     /locus_tag="PB780_000933"
     CDS             <1..1152
                     /locus_tag="PB780_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HDE0733003.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="PB780_000934"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="PB780_000934"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDE0733004.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="PB780_000935"
     CDS             2728..3003
                     /locus_tag="PB780_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HDE0733005.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="PB780_000936"
     CDS             3009..3776
                     /locus_tag="PB780_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HDE0733006.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="PB780_000937"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="PB780_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDE0733007.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="PB780_000938"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="PB780_000938"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDE0733008.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="PB780_000939"
     CDS             7920..8222
                     /locus_tag="PB780_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HDE0733009.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="PB780_000940"
     CDS             8225..9388
                     /locus_tag="PB780_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDE0733010.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="PB780_000941"
     CDS             9414..12842
                     /locus_tag="PB780_000941"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HDE0733011.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="PB780_000942"
     CDS             12863..13987
                     /locus_tag="PB780_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733012.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="PB780_000943"
     CDS             13977..14414
                     /locus_tag="PB780_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDE0733013.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="PB780_000944"
     CDS             14398..14736
                     /locus_tag="PB780_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HDE0733014.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="PB780_000945"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="PB780_000945"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDE0733015.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="PB780_000946"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="PB780_000946"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDE0733016.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="PB780_000947"
     CDS             15893..16936
                     /locus_tag="PB780_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HDE0733017.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="PB780_000948"
     CDS             17095..17823
                     /locus_tag="PB780_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDE0733018.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="PB780_000949"
     CDS             17939..18184
                     /locus_tag="PB780_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDE0733019.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="PB780_000950"
     CDS             18181..18399
                     /locus_tag="PB780_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDE0733020.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="PB780_000951"
     CDS             18535..19074
                     /locus_tag="PB780_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HDE0733021.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="PB780_000952"
     CDS             19067..21340
                     /locus_tag="PB780_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HDE0733022.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="PB780_000953"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="PB780_000953"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDE0733023.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFASYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /locus_tag="PB780_000954"
     CDS             complement(22513..22767)
                     /locus_tag="PB780_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDE0733024.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="PB780_000955"
     CDS             complement(23167..23436)
                     /locus_tag="PB780_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733025.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23906..24361)
                     /locus_tag="PB780_000956"
     CDS             complement(23906..24361)
                     /locus_tag="PB780_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDE0733026.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="PB780_000957"
     CDS             complement(24499..26427)
                     /locus_tag="PB780_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDE0733027.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26624..27457)
                     /locus_tag="PB780_000958"
     CDS             complement(26624..27457)
                     /locus_tag="PB780_000958"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDE0733028.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27649..28353)
                     /locus_tag="PB780_000959"
     CDS             complement(27649..28353)
                     /locus_tag="PB780_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDE0733029.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28376..29002)
                     /locus_tag="PB780_000960"
     CDS             complement(28376..29002)
                     /locus_tag="PB780_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDE0733030.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29328..31703
                     /locus_tag="PB780_000961"
     CDS             29328..31703
                     /locus_tag="PB780_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDE0733031.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31727..32218)
                     /locus_tag="PB780_000962"
     CDS             complement(31727..32218)
                     /locus_tag="PB780_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HDE0733032.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32405..33325
                     /locus_tag="PB780_000963"
     CDS             32405..33325
                     /locus_tag="PB780_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDE0733033.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33479..33823
                     /locus_tag="PB780_000964"
     CDS             33479..33823
                     /locus_tag="PB780_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HDE0733034.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34013..>34112)
                     /locus_tag="PB780_000965"
     CDS             complement(34013..>34112)
                     /locus_tag="PB780_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDE0733035.1"
                     /translation="KKNLGSLKAQQKNGAAIYREDEKEYYTKIKLM"
BASE COUNT        11295 a         5962 c         7060 g         9795 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttatttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgctg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgt catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa
    26581 aaaccagaac atttctcaaa taagagaagt gttctggttt tttctacatt gcaataaacc
    26641 aataaccaat caaaatgata ccaaggataa tacgatacca gccaaaagcg gtaaagtcgt
    26701 ttttcttcaa atagcttagt aagaatttga tagctaagac ggaaaccaca aaagatacga
    26761 atgttcctac aagaagaaca acagtttctt ccattttgaa tgaattccct tcagctaaat
    26821 atttgacgat cttcaagccg cttgcgccaa acatgacagg aatccctaag aaaaatgaaa
    26881 actctgtagc gacaaaacgc gaagcaccaa tcaagataga tcctaggatc gttgcgccag
    26941 aacgggatgt tccgggaaca agtgccaaga cttggaataa cccaacaaga agagctgctt
    27001 ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt gtttttgttg cgtttttcga
    27061 caataataaa ggcaataccg taaacgatca aaacaatggc aactggtaag aacttgtgga
    27121 aatttgcttc taaccaatca tccaaaggca aaccaatgat gatggctggt atgcaagcaa
    27181 cgatcacttt aaaccaaagt gtccacgtat cttttttttc taccgttgat ttacttggag
    27241 aaaatgggtt taacttgtga aaataaagaa cgacgacagc caagatcgcc cctaattgga
    27301 taacgacatt gaacatcgac ataaattgtg aactggcatc cagtttgatg aattcatctg
    27361 ctaggatcaa atgaccagta ctactgatgg gcagccattc cgttattcct tcaataatac
    27421 cgagaatcac tgctttgata atatttgcaa aaaacataaa ttccttcctt cccaattaag
    27481 atacatctta tagtataccg ttattttttg agaaacccaa tctgtttttt aatattaaga
    27541 aaaacttgag aaataaagca gataagtaca cgaagagaaa aaagaagaca aagccctcgt
    27601 tcagactttg ccttttatga ttctctggta aaaattaaaa tatccccatt atagaaacga
    27661 gtcgcgacta tgtgttttcc cgttatcgat ataagacgta tcattgatgc cgtggataga
    27721 aaatcgaccg tttgcataag tgacctttgt tacgctgcca tttaaaagcc cagcacgaac
    27781 aggctgttta ggatcgatca gattaagcaa aaaggcgatc gtcaaaccat gagatacgac
    27841 taaaacattt ccaccaccat ttttttcgca gtgatgggcg atatcttcaa atcctgtcca
    27901 tacacggtct cgcaatgttt cataagtttc cgcccaccca gcggtatcta agttgtatac
    27961 agcattagca atttgttcaa aagaaacttc tgtggtaaac atttcatcat atgttttaaa
    28021 attcaataca cgagggatca caccccacat ttccatgtca tatccacctt caagagaccc
    28081 gaaacaccat tctctaatcc ttttttcaat aaaataagga atttcttttc ccttagaatg
    28141 ttctcctaga ataatacggg cagtttcgat tgcacgacca ctgtcactag aataagcaaa
    28201 tgaaaagtcg acatttttta acccgcgacc taaataatga atgccttctt gtccttgctt
    28261 tgtgaggggt gtatcacacc agccttgtac tcgttcaatt gtattgaaca tcgtttttcc
    28321 gtgtcgtacg atataaagtg tagtttctgt cattttattc tcctcctaag tcgttttaac
    28381 gattgaaaaa aggatttgtc cttttttcct gttcaatcgt cgttgcatcg ccatgtcctg
    28441 gatagactgc aaattcatca ggtaatacaa acagctgggt ccgaatacta tgaagtaatt
    28501 gttctaaatt ccctgtatac agatcggttc taccgatact tcctctaaac agggcgtcgc
    28561 cagaaacgac aaaatcatca aaaataaagc tgacgcttcc tatagaatgc cctggggtag
    28621 ggacgacgcg aaaagacatg tttcctaaag tataagtttt catttcaaat tcatattctg
    28681 caggcgagac aataacatca gcaatatcat catgtctacc gagtccagaa agattcaaaa
    28741 tcggatcacc tagccatttt tgctctagtg ggctgacata aactgggatt tgataatacg
    28801 cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt atgggtaagc aaaatcgcta
    28861 ccggctgttg gtttgttttt ttgatttctt gtttgattcg ttccgcatct tctccagggt
    28921 caatgatcaa aagtgcttct tcgttgtaga ccaaataaca gttttcttca atcattcctg
    28981 tttttaatct ttcgatatgc atgtgcattc ctccttgata gccaaaactt ctttcatcca
    29041 ttagtataac gcattcattg ctaggaaaca aaaacaggta cttatttctc tttacttatc
    29101 tgaattatca gactatatga aaagtatttg gatattctaa agagaaggaa agtaagtttt
    29161 catagcattt taatatgtcc ttgttacatt agattcatcc aaaaatccaa attctatatc
    29221 ctatatccct gcctaatcag cagggtgttt ttttatgttt ggaaagagag ctctttttgc
    29281 taaactaggt tttttcaggt aatcttagag agaaagaggt ggtggaaatg atcaaacagt
    29341 caaaaatatc tgtgcggcac ttagtagaat ttattttgcg acgtggaagt atcgacaatc
    29401 gtaaaaaaag taaccatact gcattagaag gagcgagaat ccaccgcaag ctccaaaaag
    29461 atgcgggaga aaactatgaa aaagaagtct ttttaaaaac aacagttcaa ttagatgaat
    29521 attacctgat cgttgaaggc cgtgcagacg gtattttcaa aagagacggt atttattata
    29581 tagatgaaat caaaacctcc gaaccacgat ttgaagattt agaaccagaa caagtggaac
    29641 ttttctttca tcaagcaagg gtgtatgcct atatctatag tcaagaacat gatttacaag
    29701 aaataaatct gcaattgact tacttccaga cggcagaaga agtgatcacg agaaaggttg
    29761 aacatcagac aagagagcag ttagaaactt tttttaaaaa gctgactgaa gattataaag
    29821 aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa tgcctctttg atggctttga
    29881 aattccccca tgatgagtat aggaaaggtc agcgtgaact agcggtagct gcttataaaa
    29941 cgatccgtac gaaacagaaa ttgtttgtag aagcacctac cggaacagga aaaacaatct
    30001 ctacgttatt ccccgctttg aaagctgtag gggaagaaga gggagaaaag atcttttatc
    30061 taacggctaa aacaataact agacaagtag ctgaagatgc tatgacagca ttaaaagata
    30121 caggagctga agtaaaaagt gtcactttga cggcgaaaga caagatctgc tttttagatg
    30181 agacgacctg taatccagat cagtgcccat atgccaatgg ttattacaat cggattaatg
    30241 aaggtttatg ggatcttttg aatcatgaga atcagatcac tcgtgaagtc atcgaaactt
    30301 atgcaagaaa gcatacactt tgtcctttcg aactatcatt agatgtcagt atctggtgtg
    30361 acgtaatcat tggggattac aattatttat ttgatccaac tgtttattta agacgttttt
    30421 ttgaagatga aaaaaacgag gactatctgt ttttgattga cgaagcacat aatttggtga
    30481 atcgttcccg agagatgtat tcggcagaac tatcttatga aaaaacgaaa cgaagtaagg
    30541 aagctatacc aaaggaattc aaaaaacttc atcgccgttt caacaaacta ttaaaagaat
    30601 ttgattcgat ccgagaaata gcaaaagaag atcattggga ttatcatcat caaaaagcac
    30661 cggctgaatc tttagtaaag gcaggttatc aattaagcga aaaaattaaa gaatggctgg
    30721 cagaatttcc tgagcaccca tcacaagaac aattgcttcc ttattatttt gatttgcttc
    30781 attttttgaa ggtcagtgaa ttttatgatg atcactacga aacaacagtc gaaaaaacgt
    30841 atcgcgatct gatagtaaaa gagttttgta ttgatccaag cttgtttctt gagcagtcgt
    30901 tggataaagg aaaaagcagt attctttttt ctgctagttt ttcaccactc tcttattatc
    30961 aggaaacact ggggggacaa aaaagtctgg cttacaaact gccgagtcct ttcccagaag
    31021 gaaaccagca agttttcatc gcaaattacc tagagacgac ttatcgaaaa agggaacaca
    31081 gtctggcaaa attagtagag acgattcatg cttttgctga tggaaaggtc gggaattatt
    31141 tcgttttctt tccttcttat caatatttag atcttgcggt ggaagcattc agacagcaat
    31201 atcccgacag caatgtgcta atccaagaaa cggacatgaa tgaggaagaa cgcgaacgat
    31261 ttttaacaaa atttcaaaca aatcccgctg aaacattgat tggattttgc gtacttggcg
    31321 ggattttttc agaaggaatc gatttgagag gggaccgatt gatcggcagt atgatcgtag
    31381 gtgtaggttt gccgcagatg aatcatgagc aggaactgat caaatcatat tatgatgaga
    31441 aggaacacct tggatttgct tacgcttatc agcttcctgg aatgaacaaa gtcttgcaag
    31501 ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat cgttgtattg gcagatcaac
    31561 gattttctag tttttcttat cgacgtttat tcccacaaca ttggcaaaca gcaagagaag
    31621 tacgaagtgt tggggaattg gaagaaggaa tacagcgttt ttggttatca aaagaagcag
    31681 taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt ttttgattaa cgatagttag
    31741 tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa agcaatcaca gcttgtagat
    31801 catcacggtt cttacctgtc actctaatct gatcttcttg gatttgtgct tttactttca
    31861 gtttttcatt tttgatagct gtagtgattt tcttcgcgtt ttcacgatcg attccgttga
    31921 ccaaatcggc agtttgtcga gctttgccac ctagagcgtg ttctgattcg gaaaaatgga
    31981 tatttttgat tggcacattt cttttgatca gcttaccaaa taatacatct ttgatctgtt
    32041 cgattttgaa atcatcatcg ctcaatacaa ctagattgct gttctctaat ttgatttcta
    32101 ctgtagaatc cttgaagtca aagcgattgg acaattcttt tgttgtttgc tgaatcgcat
    32161 tttttacttc ttctgtattg acttctgata caatgtcgaa gctagcatct tttgccatag
    32221 gaaccctcct gatatacata taaatattta ccatacatct atcttatcat aggattaggg
    32281 cagattctta cctgtttgct aaaattcttc tcatttgagt tcgaagtatt ttattttaat
    32341 agataaactg ttattcttac atttggaata ccaaagtagg ggagaggtgc ttgtgagtaa
    32401 aattatgact gcgtatcata aaaatgaaag attaaagaaa atagtggtta ttttgataac
    32461 cggattgacc gctgcggtcg ctttaaatta ttttttgatt ccagccaatg tcttttctgc
    32521 agggatgaat gggatcgctc aaatcatttc ctcgctttta tatgaatggt ttcatatcaa
    32581 gatagacaca ggaacattta tttttctttt gaatatccct gtgtttgtat tagggtttct
    32641 caaagtagga aaacgagcaa cgatccttag ttttgtgaat gttgtatgtg tatcggtgat
    32701 gacgatgctg cttccagtcg gacagatcac tgataatatt ttgatgaatg ccctagtagg
    32761 cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa atgggcttta caactggtgg
    32821 gatggatatc atctctcttg tactttcaca aacaactgga aaaacagtcg gaaactacat
    32881 gctgattcta aatggtatca ttgtagtcat cgcaggcttc ttctttaact gggaaagtgc
    32941 cttgtatacg attatttcca tttatgcaat gactcaagta attgatgcca tccacacgag
    33001 tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca gaagaggtaa cagaagaact
    33061 agcaaaacgg atggtacgtg gaatgacatt actgccgtct gtcggtggct attcaaaaaa
    33121 acaagggaac atgatcatga tggtcatcac ccgctacgaa atgtatgatc ttgatcagat
    33181 cgttcatgaa atcgacgacg atgcatttat caatatttta ccaactcatt cagtatttgg
    33241 ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca actggcgtat tgccagaaat
    33301 aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt ttagaataaa tttaatctat
    33361 tacatctaat aagaacattt ttataaattt aaacgagaat cttacttcta aattctcatt
    33421 tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt gttaaggagt gtcgtacgat
    33481 gagcgaagca aaccaagaac ggtctggaca agaaatagaa ctaatcaaag aacgtattct
    33541 tgctgccttg gaaatggtga ttgatcctga attaggaatc gacatcgtca atttaggctt
    33601 gatttatgat atagaattca atcctgaaaa tggagaaaca gtcattaaga tgactctaac
    33661 aactatgggc tgtccattag cagatatttt aacagaatct atccatgacg cattaaaaga
    33721 agtgccagaa gtaacgaaag cagaagtcaa actagtctgg tatccagcat ggacgacaga
    33781 taagatgagc cgttatgcac ggattgcgtt ggggatacga tagaaatctt ggctgactag
    33841 ctatgagaag caaataggaa aaacaaaaaa gccttttatc ctttctagaa gaaaagagat
    33901 aaaaggtttc tttgtttata aaattttgtc tgctatatta taagttaatt aaataaatgt
    33961 gttctattga taaaggacat tcaaatccct ttccttttgg aatccttctt ttttacatca
    34021 gtttgatttt cgtatagtat tctttctcat cttcacgata aatcgcagcc ccattttttt
    34081 gttgtgcttt aagactgcca agattttttt ta
//
LOCUS       DAMEET010000024        33406 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.024, whole genome shotgun sequence.
ACCESSION   DAMEET010000024 DAMEET010000000
VERSION     DAMEET010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 33406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.024"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            322..630
                     /gene="rpsJ"
                     /locus_tag="PB780_000966"
     CDS             322..630
                     /gene="rpsJ"
                     /locus_tag="PB780_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDE0733036.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            661..1290
                     /gene="rplC"
                     /locus_tag="PB780_000967"
     CDS             661..1290
                     /gene="rplC"
                     /locus_tag="PB780_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDE0733037.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            1318..1941
                     /gene="rplD"
                     /locus_tag="PB780_000968"
     CDS             1318..1941
                     /gene="rplD"
                     /locus_tag="PB780_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDE0733038.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            1941..2231
                     /gene="rplW"
                     /locus_tag="PB780_000969"
     CDS             1941..2231
                     /gene="rplW"
                     /locus_tag="PB780_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDE0733039.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            2270..3103
                     /gene="rplB"
                     /locus_tag="PB780_000970"
     CDS             2270..3103
                     /gene="rplB"
                     /locus_tag="PB780_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDE0733040.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            3146..3424
                     /gene="rpsS"
                     /locus_tag="PB780_000971"
     CDS             3146..3424
                     /gene="rpsS"
                     /locus_tag="PB780_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDE0733041.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            3447..3794
                     /gene="rplV"
                     /locus_tag="PB780_000972"
     CDS             3447..3794
                     /gene="rplV"
                     /locus_tag="PB780_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDE0733042.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            3808..4464
                     /gene="rpsC"
                     /locus_tag="PB780_000973"
     CDS             3808..4464
                     /gene="rpsC"
                     /locus_tag="PB780_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDE0733043.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            4467..4901
                     /gene="rplP"
                     /locus_tag="PB780_000974"
     CDS             4467..4901
                     /gene="rplP"
                     /locus_tag="PB780_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDE0733044.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            4891..5079
                     /gene="rpmC"
                     /locus_tag="PB780_000975"
     CDS             4891..5079
                     /gene="rpmC"
                     /locus_tag="PB780_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDE0733045.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            5104..5370
                     /gene="rpsQ"
                     /locus_tag="PB780_000976"
     CDS             5104..5370
                     /gene="rpsQ"
                     /locus_tag="PB780_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDE0733046.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            5428..5796
                     /gene="rplN"
                     /locus_tag="PB780_000977"
     CDS             5428..5796
                     /gene="rplN"
                     /locus_tag="PB780_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDE0733047.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            5833..6141
                     /gene="rplX"
                     /locus_tag="PB780_000978"
     CDS             5833..6141
                     /gene="rplX"
                     /locus_tag="PB780_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDE0733048.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            6169..6708
                     /gene="rplE"
                     /locus_tag="PB780_000979"
     CDS             6169..6708
                     /gene="rplE"
                     /locus_tag="PB780_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDE0733049.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            6727..6912
                     /locus_tag="PB780_000980"
     CDS             6727..6912
                     /locus_tag="PB780_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDE0733050.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            6948..7346
                     /gene="rpsH"
                     /locus_tag="PB780_000981"
     CDS             6948..7346
                     /gene="rpsH"
                     /locus_tag="PB780_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDE0733051.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            7378..7914
                     /gene="rplF"
                     /locus_tag="PB780_000982"
     CDS             7378..7914
                     /gene="rplF"
                     /locus_tag="PB780_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDE0733052.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            8075..8431
                     /gene="rplR"
                     /locus_tag="PB780_000983"
     CDS             8075..8431
                     /gene="rplR"
                     /locus_tag="PB780_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDE0733053.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            8452..8952
                     /gene="rpsE"
                     /locus_tag="PB780_000984"
     CDS             8452..8952
                     /gene="rpsE"
                     /locus_tag="PB780_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDE0733054.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            8967..9146
                     /gene="rpmD"
                     /locus_tag="PB780_000985"
     CDS             8967..9146
                     /gene="rpmD"
                     /locus_tag="PB780_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDE0733055.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            9190..9630
                     /gene="rplO"
                     /locus_tag="PB780_000986"
     CDS             9190..9630
                     /gene="rplO"
                     /locus_tag="PB780_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDE0733056.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            9630..10925
                     /gene="secY"
                     /locus_tag="PB780_000987"
     CDS             9630..10925
                     /gene="secY"
                     /locus_tag="PB780_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDE0733057.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            10987..11634
                     /locus_tag="PB780_000988"
     CDS             10987..11634
                     /locus_tag="PB780_000988"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDE0733058.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            11825..12043
                     /gene="infA"
                     /locus_tag="PB780_000989"
     CDS             11825..12043
                     /gene="infA"
                     /locus_tag="PB780_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDE0733059.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            12076..12192
                     /gene="rpmJ"
                     /locus_tag="PB780_000990"
     CDS             12076..12192
                     /gene="rpmJ"
                     /locus_tag="PB780_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDE0733060.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            12210..12575
                     /gene="rpsM"
                     /locus_tag="PB780_000991"
     CDS             12210..12575
                     /gene="rpsM"
                     /locus_tag="PB780_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDE0733061.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            12603..12992
                     /gene="rpsK"
                     /locus_tag="PB780_000992"
     CDS             12603..12992
                     /gene="rpsK"
                     /locus_tag="PB780_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDE0733062.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            13072..14010
                     /locus_tag="PB780_000993"
     CDS             13072..14010
                     /locus_tag="PB780_000993"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDE0733063.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            14038..14418
                     /gene="rplQ"
                     /locus_tag="PB780_000994"
     CDS             14038..14418
                     /gene="rplQ"
                     /locus_tag="PB780_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDE0733064.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            14690..15529
                     /locus_tag="PB780_000995"
     CDS             14690..15529
                     /locus_tag="PB780_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDE0733065.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            15505..16374
                     /locus_tag="PB780_000996"
     CDS             15505..16374
                     /locus_tag="PB780_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDE0733066.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            16364..17161
                     /locus_tag="PB780_000997"
     CDS             16364..17161
                     /locus_tag="PB780_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDE0733067.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            17364..18110
                     /gene="truA"
                     /locus_tag="PB780_000998"
     CDS             17364..18110
                     /gene="truA"
                     /locus_tag="PB780_000998"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDE0733068.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(18443..21112)
                     /locus_tag="PB780_000999"
     CDS             complement(18443..21112)
                     /locus_tag="PB780_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HDE0733069.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(21350..21538)
                     /locus_tag="PB780_001000"
     CDS             complement(21350..21538)
                     /locus_tag="PB780_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDE0733070.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            21808..22170
                     /locus_tag="PB780_001001"
     CDS             21808..22170
                     /locus_tag="PB780_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDE0733071.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            22222..23904
                     /locus_tag="PB780_001002"
     CDS             22222..23904
                     /locus_tag="PB780_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HDE0733072.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            24021..26057
                     /gene="recG"
                     /locus_tag="PB780_001003"
     CDS             24021..26057
                     /gene="recG"
                     /locus_tag="PB780_001003"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDE0733073.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            26106..27107
                     /gene="plsX"
                     /locus_tag="PB780_001004"
     CDS             26106..27107
                     /gene="plsX"
                     /locus_tag="PB780_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDE0733074.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            27229..27471
                     /gene="acpP"
                     /locus_tag="PB780_001005"
     CDS             27229..27471
                     /gene="acpP"
                     /locus_tag="PB780_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDE0733075.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            27739..28743
                     /locus_tag="PB780_001006"
     CDS             27739..28743
                     /locus_tag="PB780_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDE0733076.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            28744..29688
                     /locus_tag="PB780_001007"
     CDS             28744..29688
                     /locus_tag="PB780_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDE0733077.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            29688..30650
                     /locus_tag="PB780_001008"
     CDS             29688..30650
                     /locus_tag="PB780_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDE0733078.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            30677..31591
                     /locus_tag="PB780_001009"
     CDS             30677..31591
                     /locus_tag="PB780_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDE0733079.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            31617..33398
                     /locus_tag="PB780_001010"
     CDS             31617..33398
                     /locus_tag="PB780_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDE0733080.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
BASE COUNT        11075 a         6052 c         7276 g         9003 t
ORIGIN      
        1 ttatttattg caggatcagg aaaaaagaca ggagtattat aaatagaaga aacacgaaat
       61 aaaagagtca gtattttcta aatgaaactt tatgaaaaca aaatgttttt tacaagaaaa
      121 agccttgctt tgtgagttta tatatattat aatgacaaag gtgcgtttac tttagtgaat
      181 tgcacagacg taatcagaat gatttcgtcg gctaagaaac gagaggttgc gacacgcccg
      241 gacgctttgc cacgaacgag cgtgacggaa attttcgtgg agctatgtct acttttcaaa
      301 ataggcgaag gagggaacaa gatggcaaaa caaaaaattc gtatccgttt aaaagcgtat
      361 gaacaccgta ttttagatca atcagcggat aaaatcgtgg aaactgcaaa aagaactgga
      421 gctagcgtgt ctggtccaat tccattacca actgaacgca gtctttacac aatcatccgt
      481 gcgactcata aatacaaaga ttcacgcgaa caattcgaaa tgcgtacaca caaacgtctt
      541 atcgacattg tgaacccaac accaaaaact gttgatgctt tgatgaagct agacttacca
      601 tctggtgtaa acatcgaaat caaattataa aactaaaaca aaaataatgg aggtgtactc
      661 atgaccaaag gaatcttagg gaaaaaagtg ggaatgacac aaatcttcac tgaatctggt
      721 gaattaattc cagtaactgt tgtagaagct actccaaacg tagtactaca agtgaaaaca
      781 atggaaacgg atggctacga agctattcaa gttggttacc aagatatgcg tgaagtttta
      841 tcaaacaaac ctgcgaaagg tcatgttgca aaagcaaaca cggctcctaa gcgcttcatt
      901 cgtgaattca aaaatgttga gctaggagaa tatgaagtag gaaaagaaat tacagtagat
      961 gttttccaag caggagacat tattgatgta acaggtacta cgaaaggtaa aggattccaa
     1021 ggggttatca aacgccacgg acaaagccgc ggaccaatgg ctcacggttc tcgttaccat
     1081 cgtcgtcctg ggtcaatggg tccagttgca cctaaccgtg tatttaaaaa caaacgtctt
     1141 gctggccgta tgggtggcaa ccgcgtaact atccaaaact tggaagttgt acgtgtcgat
     1201 gcagaaagaa atgttatctt gatcaaaggt aatgttcctg gagctaaaaa atctttaatt
     1261 actatcaaat cagctgtgaa agctaaataa ttgtaggaag aaaggaggaa ctaaggaatg
     1321 ccgaatgtag cattattcaa acaagatgga agccaaaacg gtgaaatcac acttaacgaa
     1381 gaaatcttcg gaattgaacc taatgaaagt gttgtttacg atgcaatcgt aatgcaacgt
     1441 gcgtcattaa gacaaggaac acacgcggtt aaaaaccgta gcgctgttcg cggcggcgga
     1501 cgtaaaccat ggcgtcaaaa aggaacaggt cgtgcacgtc aaggatctat ccgctcacca
     1561 caatggcgtg gaggtggcgt agtctttgga ccaacaccac gttcttacag ctacaaactt
     1621 cctaaaaaag ttcgtcgttt agcaatgaaa tctgtattgt cagaaaaagt tgctgaaaac
     1681 aacttggtag caatcgaagg cttgaacttc gacgcaccaa aaacaaaaga attcaaacaa
     1741 gttcttgcta acttatctat cgactctaaa gtattggttg ttctagaaaa aggtaacgac
     1801 tttgctgctt tatcagcacg taacttacca aacgtttctg tagtaacttc tgataatgtg
     1861 agtgtgctag acgttgtatc aaatacaaaa gtgttagcaa cacaaactgc tcttactcaa
     1921 attgaggagg tgcttgcata atgaacttac tagacgtaat caaacgccca gtgatcactg
     1981 aaaaatcaat gcttgccatg gatgacaaga aatacacttt tgaagtggac actcgcgcaa
     2041 acaaaacttt agtaaaacaa gcggtagaag ctgcttttga cgttaaagtc aaaaacgtga
     2101 acatcgtgaa cgtacgtgcg aaattcaaac gtatgggcaa atatgcagga tatacaaaaa
     2161 aacgtcgcaa agcgatcgtt acattgactg aagattcaaa agaaatccaa ttattcgaag
     2221 ctgctgaata agcactaata gataactcat taaattagga gggaaaaacg tggcgattaa
     2281 aaagtacaaa cctacctcaa atggtcgtcg taacatgaca ggttctgatt ttgctgaaat
     2341 cacaacatca acacctgaaa aatcattgtt acaaccattg aaaaaccatg ctggacgtaa
     2401 caacaacggc cgcatcactg tacgtcatca aggtggcgga cacaaacgcc aatatcgcgt
     2461 gatcgacttc aaacgtaaca aagataacgt tgttgcaact gttcaaacga tcgaatatga
     2521 tccaaaccgt tcagctaaca tcgcgttagt acactatgaa gatggagtga aagcttacat
     2581 cttagcacct aaaggactag aagtgggtat gaaacttgtt tcaggtccag aagcagatat
     2641 caaagtcgga aatgctcttc cattagaaaa cattccagtt ggtactgtta tccacaacat
     2701 tgaaatgaaa cctggtaaag gtggacaatt gatccgttct gctggtacaa gtgctcaagt
     2761 acttggtaaa gaaggcaaat acgtattgat ccgcttgaac tctggtgaag ttcgtatgat
     2821 cttagctgca tgccgtgcta caatcggttc tgtaggtaac gaacaacacg aattaatcaa
     2881 catcggtaaa gctggacgct ctcgttggat gcgtaaacgc ccaactgtac gtggtagcgt
     2941 aatgaaccct aatgatcacc cacacggtgg tggtgaaggt aaagctccta tcggacgtaa
     3001 agcaccagtt tctccttggg gtcaaccagc tcttggttac aaaactcgta acaaaaaagc
     3061 taaatcagac aaacttattg ttcgtcgtcg caaaaataaa taattacttt gaccatgtgt
     3121 cgcacatttt gagaggaggt tcaccatggg tcgtagttta aagaaaggac cttttgtcga
     3181 tgagcattta atgaaaaaag ttgaagctca agcaggggtt gaaaagaaaa aagttatcaa
     3241 aacttggtca cgccgttcaa cagttttccc aagttttgtt ggatatacca tcgcagttta
     3301 tgatggacgt aaacatgtac cagtttacat ccaagaagac atggtaggac acaaattagg
     3361 tgaatttgca cctacaagaa cttatcgtgg acatgctgct gacgataaga aaacgaaacg
     3421 ctaatttgag gggaggataa atccaaatgg cagaacaaat cacatcagct aaagcaactg
     3481 caaaaacagt tcgcacttca cctcgaaaag cccgtttagt aattgacctt atcagaggta
     3541 aaagtgtcgc agatgcaatt tcaatcttga aattcacacc aaacaaatct gctggaatca
     3601 ttgaaaaagt attgatgtca gcagttgcga atgcagaaaa taactttgac ttagatgttg
     3661 aaaacttggt agtaagcgaa gcttttgtta acgaaggacc tacaatgaaa cgtttccgtc
     3721 ctcgtgcaaa aggatcagct tctccaatca acaaacgtac aagtcatatc acggtagtcg
     3781 tatcagaaaa ataaggaggg ataatcagtg ggtcaaaaag tacatccaat tggaatgcgt
     3841 gtaggcatca tccgcgactg ggatgctaaa tggtatgctg aaaaagagta tgctgagttc
     3901 ttacacgaag atttaagaat ccgtaaattt atttcaacta aacttgctga tgctgctgta
     3961 tcaacagttg aaatcgaacg cgcaacaggt cgcgtaaaca tttcaattca cacagctaaa
     4021 ccaggtatgg ttattggtaa aggcggttct gaagtcgaaa gcctaagaaa agaattaaac
     4081 aaattaactg gtaaaagaat ccatatcaat atcgtggaaa tcaaaaaacc agatctagat
     4141 gcaaaattag taggtgaagg aatcgcacgt caattagaaa accgtgttgc tttccgtcgc
     4201 gctcaaaaac aagccatcca acgctcaatg cgtgctggcg ctcaagggat caaaactcaa
     4261 gtatctggtc gtctaaacgg agcagatatc gctcgttcag aaggatactc agaaggaact
     4321 gttcctcttc atactttacg tgcggacatt gattacgcat gggaagaagc agatacaaca
     4381 tacggaaaac taggagttaa agtgtggatc tatcgtggag aaattcttcc aactaaaaaa
     4441 aacactgaga aaggagggaa ataatcatgt tagtacctaa acgtgtaaaa caccgtcgtg
     4501 aattccgcgg aaaaatgcgc ggggaagcta aaggcggaaa agaagtagca ttcggtgaat
     4561 acggtttgca agctgttgat tcacattgga tcacaaaccg ccaaatcgaa gctgctcgta
     4621 tcgcaatgac tcgttacatg aaacgtggtg ggaaagtatg gattaaaatt ttccctcaca
     4681 aatcttatac tgccaaagca attggggtac gtatgggttc tggtaaaggg gcacctgaag
     4741 gatgggttgc accagtaaaa cgtggtaaaa tcatgtttga aatcgcaggc gttcctgaag
     4801 aagtagctcg tgaagcgtta cgtctagctt ctcacaaatt accaatgaaa actaagatcg
     4861 taaaacgtga ggaaatgggt ggtgaatcga atgaaggtta aagaaatcag agaattaacc
     4921 actgccgaaa tgcttgatca agaaaaacaa ttaaaagaag aattgtttaa tcttagattc
     4981 caattagcaa caggtcaatt agaaaacact gcacgtatta aagaagtacg taaatcgatt
     5041 gcacgcatca aaactgtgtt gcgtgaacaa gctaagtaat tatggaagga ggccattaag
     5101 cgtatgactg aaggaagaaa tcaacgcaaa gtttatcaag gtcgcgtggt ttcagacaaa
     5161 atggataaaa caattaccgt tgctgtcgaa acaaagaaaa accaccctat ctatggcaaa
     5221 cgcatgaact attctaagaa atacaaagtg catgatgaaa acaacacagc aaaagttggc
     5281 gacatcgtga gaattatgga aactcgtcca ttatcagcta caaaacgttt ccgtttgttg
     5341 gaagtagtcg aagaagcagt tattatctaa taacacgaga ttttcctata tagattgaat
     5401 gcaaaatctg aaaggaggat acacaacgtg atccaagcag aaagtcgttt aaaagtcgct
     5461 gataactcag gcgcacgtga aattttgacg atcaaagtcc ttggtggatc tggtcgtaaa
     5521 actgctaaca tcggtgatgt gatcgttgct acggttaaac aagcaacgcc aggtggggtt
     5581 gtcaaaaaag gtgacgtcgt taaagccgtt atcgttcgaa caaaatcagg agctcgtcgt
     5641 acagacggtt catatattaa atttgatgaa aatgctgcag tgattattcg tgatgataaa
     5701 agccctcgtg gaacacgtat ctttgggcca gttgcacgtg aactacgtga aaacaacttc
     5761 atgaagatcg tttcactagc accagaagtt ttataatctt gagacactta tcaaaggagg
     5821 tgcgaaacag ttatgtttgt taaaaaaggc gataaagtta aagttatcac tggaaaagac
     5881 aaaaacaaag aaggcgttgt actagcagcg ttcccgaaaa aagataaagt tattgtcgaa
     5941 ggtgtaaatg tcgttaaaaa acaccaaaaa ccttctcaag ctgctcctca aggtggaatt
     6001 gttgagatgg aagcgccaat tcatgtttct aacgtgatgg tggttgactc tactggtgta
     6061 gctggccgag ttggctacaa agaagtagac ggcaaaaaag tccgtgtttc taaaaaaacc
     6121 ggtgaagttt tagataaata aaagtttaag gaaggagggt cttactgaat gaaccgccta
     6181 aaagaaaaat atcttaaaga agtaactcca tcattgatgg aaaaatttaa ctatagctct
     6241 gttatgcaaa cacctaaagt tgaaaaaatc gtcatcaaca tgggtgttgg tgatgcggta
     6301 tcaaacgcta aaaacttaga taaagcagtt gaagaattaa ctttgatcac tggtcaaaaa
     6361 ccaatgatta caaaagctaa aaaatcaatc gctggattcc gtttacgtga aggaatgcca
     6421 attggtgcaa aagttacatt gcgcggagaa agaatgtacg aattcttaga caaattggtt
     6481 tctgtttcac taccacgtgt acgtgacttc catggtgtaa gtaaaaaagc ctttgacggt
     6541 cgtggaaact atactttagg tattaaagaa caattgatct tcccagaagt agattatgat
     6601 ttagtagata aagtacgtgg tatggacatc gttattgtaa caacagcaaa ctctgatgaa
     6661 gaatctcgtg agttgttgac acaattaggc atgccattcc aaaaataatt aaaggaggcg
     6721 aactacgtgg ctaaaaaatc aatgattgct aaaaacaaac gtcctgcaaa attttcaaca
     6781 caagcttata ctcgttgcga acgttgcgga cgtccacatt cagtttatcg taagttccat
     6841 ctttgccgta tttgcttccg cgaacttgcc tataaaggtc aaattcccgg cgtgaagaaa
     6901 gctagctggt aaaaagtaaa ttcgcataag aaggaggtaa aagatcaatg gtcatgacag
     6961 atccaattgc agattttcta actcgtatcc gtaacgcaaa catggttaaa catgagtctt
     7021 tagaagtacc tgcatcaaaa attaaacgtg acatcgctga aatcttgaaa cgtgaaggtt
     7081 tcgttcgtga tgttgaatac atcgaagatg acaaacaagg cgtgatccgt gttttcctaa
     7141 aatttggtaa aaacggcgaa cgtgttatca caaacttaaa acgtatctct aaaccaggtt
     7201 tgcgtgctta tgtcaaagct gacgaagtac caaaagtatt gaacggttta ggtatcgcaa
     7261 tcatttcgac ttctgaaggt gttgtcactg ataaagaagc tcgtgaaaaa aatatcggcg
     7321 gcgaagttat cgcttacgta tggtaatcaa gtagaatcta caaggaggtg tctttaagtg
     7381 agccgtatcg gaaataaagt cgttgttatc cctgaaggag taacagttac tcaagacgga
     7441 aacaacgtta cagttaaagg accaaaaggt gaattgactc gtactttttc agcagatatc
     7501 aaaatgaata tcgaaggaaa cgaagtaact ttcactcgtc caaacgacag taaagaaatg
     7561 aaaacaattc atggaacaac tcgtgcgaac ttcaacaaca tggttgtagg tgtcagcgaa
     7621 ggtttccaaa aagcgttaga actaatcggg gttgggtacc gtgcacaatt acaaggatca
     7681 aaacttgtat tgaacgttgg ttactcacat ccagtagaaa tcacaccacc agctggagta
     7741 actgttgaag taccagctaa cacacaagta attgttaaag gtgcgaacaa agaagaagtt
     7801 ggcgaattag ctgcaaacat tcgtggaact cgtcctccag aaccttataa aggtaaaggt
     7861 attcgttatg ttggcgaata tgtacgccgt aaagaaggta aaactggtaa ataatcgttt
     7921 agaaaaattg catagaagcc agcagcagga aaaaggcatt tggctgaagt ttaccttcaa
     7981 aaaaatacat ttttacttgt gccgcagctt ctatttttcg aaacgtattt actgcttgaa
     8041 atagtagtac aataaaaata aagaggtgac aattgtgatt acaaaaccag ataagaacaa
     8101 aacacgtcaa aagagacatc gtcgtgtacg taacaatatc tctggtactg ctgagcgccc
     8161 acgcttgaac gtttttcgtt ctaacaaaaa catctacgct caaattattg atgacgtagc
     8221 gggtgtaacg ctagcaagtg cctctacctt ggataaagaa atttcaggtg gaacaaaaac
     8281 agaagcagca caagctgtcg gtaaactagt tgctgaacgt gcagctgaaa aaggcattaa
     8341 agtagtagtc tttgaccgtg gtggatacct ttaccatggc cgtgtgcaag ctttagctga
     8401 agctgctcgc gaaaatggac tagaatttta gaagaaggag gaataccatt catggtttat
     8461 atcgatccaa aacatttgga attagaagac cgcgttgttg caattaaccg tgtaacaaaa
     8521 gttgttaaag gtggacgtcg tctacgtttt gctgctttag ttgttgtcgg tgacaaaaac
     8581 ggacacgtag ggtttggtac aggtaaagct caagaagttc ctgaagcaat ccgtaaagca
     8641 attgaagatg cgaagaaaaa cttagttgaa gtacctatgg ttggttcaac aatcccacac
     8701 gaagtaatcg gtgcttttag tggtggtaga atcctaatga aacctgctgt agaaggttct
     8761 ggggttgctg ctggtggacc agttcgtgcc gtattagaat tagctggggt agcagatatc
     8821 acttctaaat ctttaggctc aaatacacca atcaacgttg ttcgcgcaac agttgaaggt
     8881 ttgaaacaat tgaaacgtgc agaagaagtt gcagaacttc gcggcaaatc agttgaagaa
     8941 ttaatcggat aaggaggaca acaataatgg ctgaattaaa agttacttta aaacgcagta
     9001 ttatcggacg tcctcaaaac caacgcgata cagttaaagc gttaggtctt acaaagatta
     9061 acagttcagt ggttaagcct tctaatgaag caattaaagg catgatcaat actgtttctc
     9121 acctagtgga cgttgaagaa atttaattta gaaaaatcat aactagaaca ttaaggaggt
     9181 gccaaaccta tgaaacttca tgaattaaaa cctgctgaag gttcacgcca agtacgtaac
     9241 cgtgttggtc gtggtacttc atctggtaac ggaaaaactg ccggacgcgg acaaaaagga
     9301 caaaaagctc gttcaggcgg cggtgtgcgt ttaggatttg aagggggaca aacaccattg
     9361 ttccgtcgtt taccaaaacg aggattcaca aacgtgaacc gtaaagacta tgcagtcatt
     9421 aatcttgatg tattgaaccg ctttgaagac ggagctgaag taactcctgt tgctttagtt
     9481 gaagctggaa tcgtgaaaaa cgaaaaagct ggaatcaaag tattagctaa tggggaacta
     9541 aacaagaaat taactgtgaa agcagcaaaa ttctctaaag cagctcaaga agcaattgaa
     9601 gcggctggtg gatcaatcga ggtgatctaa tgtttagact attaaaagat gcttttaaag
     9661 taaaagacat tagatcgaaa atcctattta ctgtatttgt gttgtttgta tttcgcctag
     9721 gtgcacatat tactgtacca ggcgtaaatg ctgcttcatt gcaaaacctt tcttctttgc
     9781 catttttcaa catgttgaac ttggtaagtg gtagtgcgat gcaaaacttt tccattttct
     9841 caatgggggt ttcaccatac attactgctt ctatcgtcat tcagcttctt caaatggaca
     9901 tcgtgcctcg atttgttgaa tggtcgaaac aaggggaagt tggtcgtaaa aaactgaatc
     9961 aagcgactcg atatctgaca ttagtattgg catttatcca atctgtcggt atcactgctg
    10021 gattcaatca atacgctcaa ttaggtttcg tgaataatcc taacgtagca acttacgtaa
    10081 cgatcggttt gattctaaca gccggtacga tgtttgttac ttggctagga gaacagatca
    10141 cagaaaaagg tatcggaaat ggcgtatcaa tgatcatttt tgcaggtatc atttctagat
    10201 tgcctgaaag tatcagagaa atttatgaag attactttat aaatattgat ccttcagaca
    10261 tatggaaatc tgctattttt gtggccattt tactagtagc ggtcttgttg atcgtgacat
    10321 tcgtaacatt tttccaacaa gctgaacgca agatcccgat ccaatatacg aaacgcgtag
    10381 ctggcgcgcc tacaagcagc tatctgccgt tgaaagtaaa tgcagctgga gttattccag
    10441 ttatctttgc tagttcgttt attgctacgc caaatgctgt tttacaagca ttgagcggta
    10501 gattcggttc tgaaaactgg tacaaagtga tgatggagat ctttaactat aatacagcac
    10561 ctggtgctac gatttatacg attctgatcg ttgccttcac tttcttctac gcctttgttc
    10621 aagtaaaccc tgagaaatta gcggaaaact tgcaaaagca aggaagttat ataccaagcg
    10681 tacgccctgg taaggggact gaagagtatg tctcaagatt actgatgcgt ttaagtacag
    10741 ttggtgcagt cttccttggt ttagtggcat tgcttccaat cattgctcaa atggtttggg
    10801 atcttccaca atcaatcgga ttaggcggta caagcttact gatcgttatc ggtgtagctt
    10861 tagaaactgc gaaacagtta gaaggcttaa tgctgaaacg taaatatgtt ggattcattg
    10921 agtaagaaag cgtgttgagg tttcccctca acacacgttc tcacacattt attaggaggg
    10981 aaaacaatga acctcatttt aatggggctg ccaggcgcag gtaaaggaac acaagctgaa
    11041 aaaatcattg atgcttatgg tattccgcac atctcaacag gagatatgtt ccgcgcagcg
    11101 atgcaaaacg aaacagctct tggcctggaa gcaaagtctt atatggataa aggtgcatta
    11161 gttcctgatg aagtaacaaa tggaatcgtg aaagagcgac tagctgaacc agatacagag
    11221 aaaggcttct tattagatgg ctttccacgt acgctagatc aagcaaaagc tctggacgca
    11281 atgctaaaag atttgaataa aaaaattgat gctgtcatcg atatccatgt tggtgaagaa
    11341 atcttggtcg aacgtctagc cggccgattt atctgcagca attgtggagc aacttaccat
    11401 aaaattttca accctacaaa agttgaagac acatgtgatc gttgcggtgg gcatgaattc
    11461 tatcaaagag aagatgataa acctgagacg gttaaaaatc gtctggctat caacatcaaa
    11521 aatagtgaac caatcttggc ttattataaa gaacaaggtt tgctaaacac tattgatggt
    11581 gctcgtgaaa tcgatgctgt atttgcagat gtacaaaaaa tcattgaaaa atagcacgat
    11641 tgcccgtagc cgacttgcat tatgcagtta ctcttgtcaa gttcaataaa ttatgataga
    11701 atatgtcagt ccgacttcta gagaatcctc tagatgatcc attttgagaa ttttgaggtg
    11761 ggaactgcag tttccgccat tttatcgtgt gaaaatgcga aacaagtaca aggaggtact
    11821 gtgcgtggca aaagaagata tgattgaagt cgaaggtaca gtcgtcgaaa ctttgccgaa
    11881 tgcaatgttt aaagttgaac tagaaaacgg acaccaagtt cttgctactg tttcaggtaa
    11941 aattcgtatg cattacatcc gtatcttacc tggtgacaaa gtaacggttg aattgtctcc
    12001 atacgatctt aaccgtggcc gtattactta tcgctttaaa taattgtact ccgtaaaact
    12061 acaggaggta ttatcatgaa agtaagacca tcagtaaaac caatgtgcga acattgtaaa
    12121 gtaattcgtc gtaaaggacg cgttatggtg atttgcccag caaatccaaa acataaacaa
    12181 cgtcaaggat aattggaggt gctataaata tggctcgtat tgcaggagta gatattcctc
    12241 gtgataaacg tgtagtagtt tcacttactt atatctatgg tattggtaac actactgcta
    12301 aaaagatctt agctgatgct ggtgtatctg aagatgttcg cgttcgtgaa ttaacgaatg
    12361 aacaaacaga tgctatccgt gcagaagtag ataaattaaa agttgaaggg gatcttcgtc
    12421 gtgaagtgaa cttgaacatc aaacgtttga tggaaatcgg ttcttaccgt ggaatccgtc
    12481 atcgccgtgg attgcctgtt cgtggacaaa acacgaaaaa caatgcacgt actcgtaaag
    12541 gcccagctcg cgctatcgct ggcaagaaaa aataattctt aagtgaagga ggttaaaact
    12601 tcatggcagc aaaaaaagtg aatcgtaaac gccgtgtgaa gaagaatata gaagcaggta
    12661 ttgcacatat ccattctaca ttcaataaca ctatcatcat gatcactgat actcacggaa
    12721 atgcattagc gtggtcatca gctggttcat taggtttcaa aggaagcaaa aaatcaactc
    12781 cttttgccgc tcaaatggca gcagaaactg ctacaaaagc agcaatggaa cacggattaa
    12841 agactgttga agtaacagtt aaaggacctg gttctggacg tgaagcagca attcgttcat
    12901 tacaagctac aggtttagaa gtgactgcaa ttcgtgacgt gactccagtt cctcataatg
    12961 gtgcccgccc tccaaaacgc cgtcgtgttt aatgagtgcc gctcattttg agttttatca
    13021 gtaacgtcat tgaacaagac acttttcgtt ttgaaagggg taaagataag aatgattgag
    13081 ttcgaaaaac caagaatcgc aaaaattgat gaagaaaaag attatggcaa gttcatcgtt
    13141 gaacctcttg aaagagggta tgggactact ttagggaatt ccctacgtcg tattttatta
    13201 tcttctttgc caggtgctgc catcactaat attcaaatcg atggtgtact gcatgaattc
    13261 tctaccatca aaggtgttag agaagacgtc actcaaatca tcttgaacat taaaggctta
    13321 gcgctgaaaa tgtatggcca agaagaaaaa acccttgaaa tcgatattac cggacctgct
    13381 acagtaactg ctggcgatat tatcgttgat agtgatgtag aaattcttaa caaagatatg
    13441 tacatctgta gtgtctctga aggagctacg ttccatgctc gcttaacagt gaaacctgga
    13501 cgtggttatg ttcaagcaga tgaaaataaa aaagaagata tgccaattgg cgttcttcca
    13561 gtcgattcaa tctacacacc tgttcgtcgt gtgaactatc aagttgagaa cacacgtgtt
    13621 ggacaccgtg aagattttga taaattgacg atggagattt ggacagatgg ttcaattgaa
    13681 ccgttggaag caatgagttt gtctgcgaaa atcatgactg aacatttgga tatttttgtg
    13741 aacttaactg atgaagcaaa aaatgctgaa atcatggtcg aaaaagaaga aacacaaaaa
    13801 gaaaaaatgt tggaaatgac gatcgaagag ttagacttgt ctgttcgctc ttacaattgt
    13861 ttgaaacgtg caggaatcaa tactgtacaa gaacttacaa ataaatctga gcctgaaatg
    13921 atcaaagtcc gtaacttagg acgtaaatca ttagaagaag ttaaagcaaa attgcacgac
    13981 ttaggtttag gtttacgtaa agaagattaa aaccttttac gaaggaggga aaccaacgtg
    14041 agttatcgta aactaggacg cacatctagc caacgtaaag cgatgttgcg tgatttaaca
    14101 actgatttat taatcaacga acgtattgtg actactgaag ctcgtgcgaa agaagttcgc
    14161 tcaactgcag aaaaaatgat cactttaggt aaacgcggag atttgcatgc acgtcgtcaa
    14221 gctgcggctt tcgtacgtaa tgaagttgcc agcgtgcgtg aagaaaatga ggacatcgtc
    14281 attgaatcag ctttacaaaa actgttcaac gatattgcac ctcgttacgc agaacgtcaa
    14341 ggcggataca cacgcatttt gaagacagaa ccaagacgcg gtgatggtgc accaatggtt
    14401 gtcttagaac ttgtctaaaa tcacactaat aacatcactt tatagatgat tcttttagag
    14461 tgttatgatg ttggaagata tttttccgag tctagctcag cgctgctcct caagcgaata
    14521 tcgcttgggg agtaagcact catcataaag tgttttttta taccttcaat tacctgtcat
    14581 gacagatatg tttgaaggca taaaaaaaca ttttaagcga gaaataattg aaaattttgt
    14641 ctttttttga tagtgttagt aggaacttta ggatgaagaa ggagtaatca tggagccaat
    14701 tattgaatta gaaaagataa attataaata ccagccagat gatcttcgcc ccgcattgaa
    14761 agatgtctcc tttacaattg ataaaggtga gtggatcgct attattggac acaatggttc
    14821 aggaaaatcg acacttgcca aaacgatcaa tggattgctt ctgccagaat cagggatcgt
    14881 gaaagtggga aatcaaatat tggatgaaga aaatatttgg acgatccgac agatggttgg
    14941 aatgattttt caaaatccag acaatcagtt tgttggttcg acagtagaag atgacgttgc
    15001 ttttggtctt gaaaatcaag ggattcccag agaagaaatg ctagtgcgtg tgaaagatgc
    15061 acttgagaaa gttagaatgg ctgagtttgc ttcacgcgag cctgctcgtt tatcaggagg
    15121 gcaaaaacaa cgtgttgcca ttgccggcgt ggttgcctta cgacctgata tcattatttt
    15181 agatgaagca accagtatgc tagatccaga aggtcgtgaa gaagtgatct ctacgatcaa
    15241 gaagatcaaa gaagaaagtc aattgaccgt tatatctatc actcatgata ttgatgaagc
    15301 agctaatgcc aatcggatac tagtgatgag acaaggcgaa cttgtccgtg aaggtacacc
    15361 aaaagaaatt ttttctgctg gtcctgaact gatcgactta ggtctagatc taccatttcc
    15421 tgaaaaactg aagagcgcac tgaaagaacg tggggttgat gtgcccagtg aatatatgac
    15481 agaagaaagg atggtggatt ggttatggac atccgttttg aacaagtaga cttcacttat
    15541 cagccgaata caccatttga acaaagagcc ttatttgaca tcaatatgac gatcaaagaa
    15601 aacagttata ctgcattagt agggcatacc ggaagcggga aatcaacctt acttcagcat
    15661 ttgaatgcgc ttgttaagcc aacaagcggt acagtccata ttggagaacg agatattcag
    15721 ccggatacgg ataataagaa tttaaagccc atccgaaaaa aagttggcat cgtctttcag
    15781 tttccagaag cacagctatt cgaagaaacg gtagcaaaag acattgcttt cggtcctaaa
    15841 aactttggtg tcagcgaaga agaagcacta gtcctagcaa aagaaacatt agaacaagtt
    15901 gggctggatg aaagctattt ggaacgttcg ccatttgaac tttcaggagg gcaaatgcgt
    15961 agagtagcaa tcgctggtgt gcttgccatg aggccggaag tacttgtatt ggatgaacca
    16021 acggcaggac ttgatcccca gggcagaaaa gaaatgatgg agatgttttg gcgtcttcat
    16081 aaggaacagc agatcactat tgtattagtt acgcatttaa tggatgatgt agccaatttt
    16141 gcagattatg tctatgtgtt ggaaaaggga cgaatcgtta atagcgggga gccgcaagag
    16201 gtctttcaaa acattgaatg gctaaaagaa aaacaactag gcgtacctac tgccaccgag
    16261 tttgcagaag aattaatggc taaagggatg aactttgcta ccttgccttt gacagctgaa
    16321 gaattagcag acgctatcgt ccattatgct ggaggaaatg cccatgatga ataaattgat
    16381 atttggtcgt tatatgcctg gagattcctt gatccatcga ttggatcccc gagctaaatt
    16441 attagccagt ttttatttta tcggaatcat ttttttagca aataattggg aaacgtacct
    16501 tttgttggca tttttcacat tactttctgt cttcttgtca aaagtagatt tgaaattttt
    16561 tatccgaggc attcgcccat tgatctggct aatcctcttc actgtagctt tgcaggtctt
    16621 gtttacaagt ggcggagaaa cttattggag ttggggtgtt ttccatatca ctgaatttgg
    16681 tgtgatgaac ggcctcttta ttttctgtcg attcgtgttg atcattttta tgtcgactct
    16741 actcacgctt actacaccgc ctttggaatt atcggatgcg attgagtact tgcttcgtcc
    16801 attgaaagcc gtacggttcc cagtccacga aatttcattg atgctgtcga ttgctcttcg
    16861 ttttgtgcct acattgatgg acgaaacaga aaagatcatg aacgctcagc gcgctcgcgg
    16921 tgttgatttt ggtgaaggaa acctagttca gaaaatgaaa gcagtcattc ctttgctgat
    16981 tcctttattt gtcagcagct tcaaccgtgc ggaagatctt gcgactgcta tggaagcaag
    17041 aggctacaaa ggtggggaag gaagaacgaa atatcgtgta ttgcattggc ataatagaga
    17101 cacgatggtt atcattgctt ttgtattatt aactgtgcta ctgatctttt taagaggata
    17161 aacgttcagt gggccgctat ctattctttt cttgctgatc tgtttgcaag aaaggatgca
    17221 agcagtgtat gacaagaaat attagggtct gtggcagact aaggtcacag gcctctttct
    17281 tttatctgaa aagaaagaac gaaaaaattt tatagaaaaa tcattattcc agataacctt
    17341 ttaggaacaa aggagagaaa aacatggtac gttacaaagc aatcattgcg tacgacggaa
    17401 caaattttaa tggattccaa aaacagccaa atgggcgtac tgttcaagaa gaagtagaaa
    17461 aaacattaca aaaaatggca aatggaaaag aaatcacggt ctttggttcg ggaaggacag
    17521 atgccggagt acatgcgatc gggcaagtaa tccattttga ttatccagag gagcgtccgt
    17581 tagaacgaat gcgtttcgcg ctagataccc agtcaccaga ggatattgct gtaagacaag
    17641 tagaaattgt ttcagaagac ttccacgcaa gatatttggt caaagaaaag acgtatcaat
    17701 ttcgagtgga tatagggaaa ccacgcagtc cttttcgacg ccattatgca agttattttc
    17761 cttacccact tgatttggaa aaaattcagc gggcgcttcc tgatttatta gggactcacg
    17821 atttcacctc tttttgtgca tcaggaagct cgatagagga taaaatacgt acgatctacg
    17881 aggctaagat ggaggtaaat gaaacaaaag acgaattgtt gtttacattt aggggaaatg
    17941 gcttcttgta taagatgata cggatcttag taggaacgct tttgaaaatt ggcaatggcc
    18001 gattgcctga agatagtatc ccagatatta ttgcgaaaaa agatcgtaat gcagcaggac
    18061 ctacagcaca ccctgaagga ctttatttgt atgaagtgaa atatgaataa gaggttatga
    18121 tagaagtggt cagcttcaag aaataagaag gtatttacga aaattacttc tcaaaatttt
    18181 gcaaaatttc ggcttatttt cgaagaagct acttctggaa caccgtttaa gaaagaggtc
    18241 gtgagcctgt catttaactc tgagtcacaa agagcaattt caaaaaacag cttctcatct
    18301 ttgatgaata tcgtgacttg tggacgaggc gttgataagt gaatcatctt tttgttacct
    18361 attttttctg taaccaaaaa acgatggaac gttgttttga ttgcccttgg tgcggacagt
    18421 ctgaacaggt tccatcgttt ctttattttc tacgtcttct tctgtgtgtg tttcgtggtt
    18481 gttcttctat tgctggatca atcgtgccat aaaaggggcg attgcggtca aggtaccaga
    18541 cataaagaat aaacaatgca atacatagga caaaaaggat tgcacctatc gtaaatcctt
    18601 caggcaagta actgaaagtg acagtatgtt tacctgctgg aacttttatc atcaagaatg
    18661 cgtctttgaa attgctgacc ttgactggtt tcccatcaat tttagctttc catcctttgt
    18721 cataaggaat agttgtcacc aacatggaat cttcagcggt atttacagtc ccagaagctt
    18781 ttctgccagt ggcttccatt tcgacacctt tttctttgat ctgattcatt gcggcttgat
    18841 aggctacaac atctagacca acgactttag gctccatgaa acttatttct ttcgtcccat
    18901 aaaaacttgc agtaaatgtc actgttgtct cttgaggata atagccaaga tcatagtatt
    18961 gaccagaaat gttgatttgc gtttttcgcg atgtaccatt gacagtgatc gtcgcactag
    19021 aactttctaa ttgcgcaaaa ttggttggga acaaacttaa gtaagcttgt gtatttgcag
    19081 gcacatggac ttcccaagtg agctcttttg gctggttatc tgctgtttct gagtaagtga
    19141 cgccagtcgt tgtctttgtg atcgtcgtat tcaactgatt cttcaaagta ggtgtataga
    19201 atgtaaaata ctgttcattt agaccagaca atgtattgat caagtttgtc tgactggtca
    19261 aattatctgt cactggctgc tcaacatcat taacggacaa cggtgcagta aatgctagtg
    19321 gcaatgcatt gacattctga tacagtgtat agtcgccgct ttgaccagct tggcggaagc
    19381 catatttact gaacgtgctt tcgtttttag aaatgttata tttgattcct gtaaacccat
    19441 ccatcagcaa agtattattg ttataacgga tattcaaatt tgtccctctt gaacggaaac
    19501 ctaatttatc caggtaagag gaagaattac gatttcttat agaagaaaac aaactgatac
    19561 cactgtaacc atagttgatc gaatcgtttg cagatactgg atccagattt tccaagcggt
    19621 aaaacgagtc ggaatctttt tttgtttcat ttacaagagc tttcaagtca ggataaggat
    19681 cagtatataa actgcgggaa gcataattcc aatcatcaag aatgccatgg atcatactgt
    19741 tcgtattgac cagtgcttca gcagacatga tcaataataa caatatactc agatgattga
    19801 atgaaagttt tttatgctga taaaatccca cagcaaaagc ataaatccct aagaaaacca
    19861 ctgtcaatac aaaggaagtc atcgtcacat aggtgtagct tccttcaggt ttgatgcccc
    19921 atgccaccgc aaagagagca gctacgacca aaatgattcc taacaacagc ccaccatcgt
    19981 cttgttctat cttttcccat ccttttcctg caagcaggag cactaagaaa gagaacagat
    20041 aggcataacg gaataaaaac atattcggtg catgcatacc atgccagaac aaattcaatg
    20101 gaacaagata aaaacttgcg ataagtatgg cgaaaagact gccgtacaaa aatttatttt
    20161 tcaacggtac tttccgactg gcaaaataaa acagacagag gactaatggt agcagacctg
    20221 cgtaaacgaa cggaatcgat ccgtattttg tcgtgtcata aacaccaatc atatttttca
    20281 tgattagatc cagataagaa gtcgcttctg ttttcaacgt agtgatctga ctcagtgcct
    20341 cgccatttga ccgaagatcc agcactgctg gcaatacaat gatcattgaa gcgccgcctg
    20401 ccagtaaaga agtgatacca taaggaacaa tcgttttttt atattctgac cagtttgtca
    20461 atagacgggc gataaaatat aagaaagaaa atacgccaat cataaaaccc atataaaaac
    20521 tggataaaaa caagagtaaa taactgataa ataaaagttt cggccgttta agatccataa
    20581 cacgatgaat ccccaaaatc actaatggaa gataaacaaa tgcatccagc cacatgatga
    20641 tctccgaatg tgcagtgata aatgagatca atgcgtaact gacgctaagt gcaacatgcc
    20701 cccagcgagg aatcttgaac gtacgtgcaa gaaaccaaaa agccaatccg gcacagccga
    20761 ccttaagcaa agtcagaaca tacagtgcat ctggcatcat ctggtttgga aaaaagaaca
    20821 ccagtggcgt gaagatcccg cctagatagt aagaaattaa agataaataa ttcaatccta
    20881 gagaagcatt ccatgtataa aaaatacttt gctttccaag aagaacattt cggaagctgg
    20941 catgaaaatt agaaaactgt gaaaaggcat cacttgccaa aatacttctg ctgcttccag
    21001 gatagattcc gatacttaga taaacaagtg ctaaaatcaa aaacggaatc aggaaactag
    21061 ctgctgcgta gcgtccgttt tctctaaaga atgactttaa attttttttc atatttattt
    21121 tccaccaaat ttctatgaag ttgtattcag tttatcatat ccttgttttt ctctgcttaa
    21181 aaagagtcta gttttcacaa aaaaataaaa tttagaataa ataacttcat aatttgttcg
    21241 tataacaaaa ataaacgcaa aaaaagggta ggaatcaaaa gtcccaccct taaaaatttt
    21301 tattaaataa aaaaccgttc actatctgaa cggtccaagt atcattcaat taaacgcgtt
    21361 caacttttcc tgatttcaaa gcacgagttg acacccaaac ttttttaggt tttccgtcga
    21421 ccataacgcg aactttttgt aagttaggtt taacagtacg ttttgttgag ttcatagcgt
    21481 gtgaacggtt gtttccactt tttgtcttgc gacctgtaaa gtaacaaact tttgccatgg
    21541 taatttcctc ctttgccttg atttggagca atagaataat tcagctcata ccagactaac
    21601 atatcatata gaatgggaat ttgcaagtac attttcatag atacaattga caattgagaa
    21661 gaataattgt aaattaaaca aggttatacc tgaaattttg caaggttttt gagtactttg
    21721 ggtaactgcc ttttatttca agttgcgcta tgataaaata tgatagaaga aaaatgggag
    21781 ttttttaccc gttaaggagg ctcattcatg gctgtaaaaa ttcaaacgtc agctggaacc
    21841 attgaaatta ccaatgatgt aattgcaaca gttgttggtg gcgccgcgac agatgtgttc
    21901 ggtgttgtag gaatggccag caaaaatcaa attaaagata atattactga gattttgcgt
    21961 aaagaaaatt actcaagagg agtagttgtc cgccaagaag ataatggtat tgctgtagat
    22021 gtttatacga ttatcagcta tggtacgaaa atctctgaag tttcccgcaa cgtccaagaa
    22081 aaagtgaaat acaatctaga ggcattatta ggcgtaactg ccaattcagt taatgtcttt
    22141 gtacaaggtg ttcgtgtact gccagactag gcaggagact ggctggtaac tcagcagtcg
    22201 atctgaagga ggattatcta ggtgagtgta acagaaataa gtgcaagcca gttccaggaa
    22261 atggtccagg caggtgcgaa tcgtctgcaa gtaaatgcag aatacgtcaa ttcattaaat
    22321 gtattccctg tgccagatgg cgatacggga acaaatatga atctatctat gactagtgga
    22381 gctaaggcag tagtagattc aacctcagaa aaagtaggag aattagcagc aatgctttca
    22441 aaaggattgc tgatgggtgc gcgcggaaat tccggcgtta ttttgtctca attgtttcgt
    22501 ggattttcta agcaaattcc agatgttgtc tcattaaatg cttctgatct tgctgcagcc
    22561 ttcactcatg gagttgaaac tgcgtataaa gcagtaatga aaccagtaga aggaacgatt
    22621 ttgacagtag ctagagaagc agcgaaagct ggagaaaaga aagcaaaaag tactgatgat
    22681 gtcgttgaag taatgacagc tgttgtaaaa ggagcaaagc gtgctttagc taaaactcca
    22741 gacttgcttc cagtcctaaa agaagtcggt gtcgtagaca gtggagggca aggactgctg
    22801 tttgtttatg agggcttttt aagtgcattg aatggagaat tccaagcgga tgaaacatat
    22861 gaaccttctc cagcagaaat ggatgatatg gtcaatgcag aacatcatcg cagtgtccaa
    22921 ggtcaaatgg cgacagaaga tatccatttt ggttactgta cagaaatcat ggtaaaaatc
    22981 ggagaaggac ctacagtcga tagtacattc gattacgaag aattccgtaa ctatttagat
    23041 ggtataggag attctttact tgtggtcaat gatgacgaga tcatcaaagt ccacgtgcat
    23101 acagaaaacc caggagaagt cttgaattat ggacaaaaat tcggttcatt agtcaaaatc
    23161 aaagtcgaca acatgcgctt gcagcatgag acgattttgg aacacgatga agaagtgaac
    23221 gagtttgcga aaaaagaaat accacgtatc ccgtatggta tcgtagcgat cgctgctggt
    23281 aaaggcgtcc aagagctgtt tgaaagttta ggcgctaatt atgttatcag tggtggacaa
    23341 acaatgaatc caagtacgga agacattgtt aaagcgattc aagaagtaaa cgcagataaa
    23401 gtgattgtct tgccaaacaa taagaacatc ttcatggcag ctgatcaagc agcagaagta
    23461 gcagatatcc cagtagcagt tgttccttca agaacgattt cccaaggatt gacagctatg
    23521 ctttcattca atgatcaagc ttctttagaa gaaaacaaag aaacgatgac agaaatgttg
    23581 tctagcgttg tgagcggtca agtcactcac gcgatcagag atacctcaat tgacggtgta
    23641 gagatcaaag aaggagactt tttgggcatg atcgatggaa aaatcctgat ctctaaccca
    23701 gatatcacag caacagcgtt aagtacacta gaacaaatga tcaatgagga cacagagatc
    23761 gtaacgatct tggcaggtga tgaaggaaca gttgaacaag caaatgaatt tgctgagaaa
    23821 ctaacagaaa aacatcctga tttggaaatc gagatccacc aaggagatca gcctgtctat
    23881 ccttatctat tttctgctga ataatttctg tgtcagaata tctttctaac acagtattca
    23941 ttcataaaag gccttcggtt agtgatcagg tatgatcgct gaccaagtcc tttttttgtt
    24001 aaaggaggga ggaaaaattt atgcgttctt tagatgatcc aatcactatg ctggcaggag
    24061 tcggagaaaa aagagcagct agtcttgcca gcttgggcat ccaaacgatt gaagacttac
    24121 tgacttatta tccttttcgc tacgaagata ttcaggaaag aaatctaaac gagatccagg
    24181 atcaagaaaa ggttactttg aaaggaatcg tagtctcgcc tcctgtgatg aatcgatttg
    24241 gttataaaaa aagccgattg caatttcgta tgatgcaaga acatgatgtg ttcaacgttt
    24301 ctttttttaa tcaaccttat ctaaaagaaa aagtcatttt atcggaggaa atcgccgtat
    24361 acggtaaatg ggatgcaaag cgtaaagcct tgaacgggat gaagatttta ggttctcaaa
    24421 caatggatga cttttcgccg atatatcacg tcaacaaatc aattcgtcaa acgacattag
    24481 ttgatctaat caaaaaaggg tttcaagaat acggcacatt gattgaagaa aatctgccaa
    24541 acaaccttgt ggagaaatat cgactgcttg accgagcttc tgctgtaaga agtatgcatt
    24601 ttccaaaaga ccacgaagaa aaccatcaag ccaaacgacg tgtcgttttt gaagagttct
    24661 ttttatttca aatgcgtctt caaggattga aaaaagcaga aaaagcagaa acgaatggtt
    24721 tggagatcct ttatgatgtc caaaaattga aacaatttac tcaaaagcta ccttttgaac
    24781 tgacaaatgc acaaaaacgt gtaacgaatg aaatctgtcg ggatcttcgc agtccgcagc
    24841 atatgcagcg acttttgcag ggagatgtag gtagcggaaa aacagtggtt gccgcgattg
    24901 cgttatacgc taccgttaca gcaggtttcc aaggtgcgtt gatggtgcct acagaaatct
    24961 tggcacaaca acatatggaa agcttgcaac agctgtttga tcccaatgaa gtaaccatcg
    25021 cattgattac ggggtcaacg aaagcaaaag aacgacgtga gctccttgaa aaactagagc
    25081 aaggggaaat taacgtcgtg atcggcacac atgcattgat ccaagatgga gtcgaatttc
    25141 agaatttagg tctagttatt acggatgagc agcaccgttt tggcgtgaac cagcggaaag
    25201 tacttcgtga aaaaggctgg cgaccagatg tcttatttat gacagcgact ccgattccta
    25261 gaacattagc tattacagca tatggtgaga tggatgtatc tatcattgat gaacttccag
    25321 ctggacgtat cccgatcgaa acacgctggg taagaacacc tcaattggac agtgtactgg
    25381 attggacgaa aaaagaactg gcaagaggac atcaaatgta tgtcatctgt ccgctgattg
    25441 aagaatcaga agcattagat gtaaaaaatg cagtggaaat ctatgaaaaa cttcgtgaac
    25501 tatttgcacc aaaatatgaa gttggcttgt tacatggtaa aatgaagaat caggaaaaag
    25561 aagtaatcat ggaagccttc aaagacaacc agatgcagat attagtttcg actactgtaa
    25621 ttgaagtagg ggtaaacgtg ccgaacgcga ccgtcatgct gatcatggat gcggatcgat
    25681 ttggattggc acagctgcac caacttcgtg gacgtgttgg acgcggaagt gatgcttcct
    25741 actgtatctt agttgctaat cctaaaaatg aactaggtgt ggaacggatg aaaatcatga
    25801 cagagaccac caatggtttt gtactaagcg aaaaagatct ggaattacgt ggtcctggtg
    25861 aagtatttgg cttccgtcaa tctggtctgc ctcaatttgc gattgctgac ttggtgacgg
    25921 atggaaatgt tttggaggta gccagagaag aggctgcagc cctttggcaa ataaaggatt
    25981 ggcagatgct tcctgagtat cagcctttag cgcaaagcct ccaaatagaa aatcaggata
    26041 atcgttattt cgattagcag actgtgctat actttatact gatgacggaa aaaggagtgg
    26101 aaacaatgaa aattgccgta gatgcaatgg gcggagatca cgcacctcaa gcaattgtag
    26161 aaggcgtggc tttagcgcag caagattttc cagaagtaga attcttactt tacggaaaag
    26221 aagcagaaat caagaaatac ttaacaaatg aaaaaaacat cacgattatc catacagacg
    26281 aaaaaatcaa tagtgatgat gagccagtaa aagcaattcg acgaaaaaag actgcatcaa
    26341 tggtccttgc tgcacaagca gtgaaaaacg gtgaagcaga tgctgtattt tcagcaggga
    26401 atacaggcgc gctactagca gcagggttgt tcatcgtagg acgtatcaaa aatatcgaac
    26461 gccctggctt gatgtctacc ttgccagtag tagggaaaga aggtgccggt ttcgatatgc
    26521 tggatcttgg cgctaacgca gaaaacaaac cagaacattt gttgcaatac ggtattttag
    26581 gttcttttta tgcacgtaaa gtccgaggta tcggacgtcc tcgtgtcgca ctcttaaaca
    26641 acggaacaga agcaacaaaa ggtagtgaag tgacgaaaaa agcctatgaa ctccttcaga
    26701 atgaaacaag cttgaatttt attggaaacg tagaagctcg tgagctactc aatggtgtgg
    26761 ctgatgtagt ggtcacagat ggatttacag gcaacgctgt tttgaaatct attgaaggaa
    26821 cagcaatgaa tatgatgaat ctattaaaat ctgctattct aaatgaaggg atcaaaggaa
    26881 aaatgggcgc tatgctttta aaagacggct tacgttcttt gaaagcagaa atggattatt
    26941 ccaaacatgg cggtgccgtt ttgtttggct tgaaagcacc agtcattaaa actcatggag
    27001 caacaggccc cgatgctgtt cgctatacga tccgccaaat ccacacaatg cttgagacag
    27061 atgtagttgg acaattagtc gaacaatttg aaaaaagcga agaatagtat ttggatatct
    27121 gaagcgatcc gttccattga aaaaaaggtg gacattcctg aaaaatgcca gtaaaattag
    27181 ccatggtgta ggtaattgac aagtgtatga ttcttggagg tgaaaaagtt gactcgcgaa
    27241 gaagtattta acaaagtagc gaaaattatt tcaaatcatt ttgagataga taccgataaa
    27301 gtgacggatg aattgaacat caaagatgat ttaaaagctg attcaattag tattatggaa
    27361 ttcgttttag agcttgaaga tgaattcggt acggaaattt cagatgaaga cgctgaacaa
    27421 attgaaacag tcggtggcgc agtagattat atcagcagcc atcttaaata atttcaaata
    27481 aaagcaaaga aggataaaaa gctgcatgca gctttttatc cttctttttt taatcttttt
    27541 gacacgtaaa agtcattttt ctgataattc tggcgataaa cagaaattat gttgcaaagt
    27601 aaagcggttt tctatataat tattaataat tcattacaca aagataagaa taacctttgt
    27661 tataaaacga tttattctgt gtaatgtcct tttttattaa gaaaaacttc catttaggaa
    27721 gcagagaggg gtattcgatt gtccgataac caattattag aagttcagaa cctacataca
    27781 ggattccgac tcaaagatga ctattacgat gcagtagatg acgtttcatt tactttagac
    27841 aaaaacgaga ttttagcaat cgttggggaa tctgggtgcg ggaaaagtac cttggctact
    27901 acgattatgg gattacttga tcctaataac acaaaaatta caggtgagat tttatataac
    27961 gatctgaatt taatcgacct gaacgaaacg ctctataata aaatccgcgg aaatgatatc
    28021 ggaatgattt tccaagatcc gttgtctgca ttaaatcctc tcatgagaat cgaagaccag
    28081 atcaaagaag gactttccta tcacacaaag atgaatgcag aacagcgtca agctcgtgca
    28141 ctagaattgc tagaacaagt agggattcct aatcctgcga gagttggacg ccaatatccc
    28201 catgaactat caggcggtat gagacaacgt gtcatcatcg caatcgcaat cgcctgcaaa
    28261 cctccaatcg tcattgcaga tgaaccgacg acagccctag atgtaaccat ccaagcacag
    28321 attcttgatt tactgaaaga ccttcaggaa gaaacaaaaa cagggatcat cttgatcact
    28381 catgacttag gagtcgtagc agaaatggct gatcgagtgg cagtgatgta tggcggacag
    28441 ttcgttgaag tagcaggtgt aaaagaactg tttgatcatc ctaagcatcc ttatacacgc
    28501 tctttactga actctatacc acaagaagga aatcatgatg cagaactcca tgtgatcgaa
    28561 ggtgtcgttc cttctctgaa aaacatgcct cgtaaaggtt gtcggtttgc accacgtatt
    28621 ccatggatac cagcaagcga tcatgaagaa gagcctatcc ttcatgaagt cgcaccgaac
    28681 cacttggtac gttgctcctg ctataagcat ttccacttta gagacgaaaa aggagaggtg
    28741 tagatgacag aattaatcac aatcaaagac ctgaaagttc attaccctat tcgcagtgga
    28801 ttcttcaatc gagtaacgga tcatgtatat gctgtagatg gtgtggattt tattatcgaa
    28861 aaaggaaaga catatggttt agtgggagag tctggctctg gaaaatctac tacgggaaaa
    28921 gccgttgtgg gattagaaaa ggtaacagcc ggacaaatca tgtatgaagg aaaagatgta
    28981 acgaagcgaa gcaaccgcaa aaaaatgggt tataacaagg atgtgcagat gatcttccaa
    29041 gattcaatgt ccagtttgaa tcctaaaaag cgagtgttgg atatcattgc tgaacccatc
    29101 cgaaattttg aacggttaag cgatcaagaa gaaaagaaaa aggtaaaagg actgctagat
    29161 atcgttggga tgccggaaga tgcgctatat aaatatcctc atgaattttc aggcggacaa
    29221 cgtcaacgtc taggagtagc ccgtgctgtt gctaccaatc caaaactgat cgtagccgat
    29281 gaacctgtat ctgctctgga tctatctgtt caagcgcaag tattgaattt catgaaacga
    29341 atccagcaag aatacggcct cagctattta ttcatttcac atgatttagg tgtagtaaaa
    29401 cacatgtgcg acaatatcgc aattatgtac aaaggtcgat ttgtagaaat cggtacaaga
    29461 gaagacattt ataatgaccc gcgacatatc tatacgaaac gtctgctttc agctattccg
    29521 caaatcgatg tagaaaatag ggaaagccac aaagaaaatc gacgaagagt agaacaagag
    29581 tatatccaaa accaaaaaga atactatgat gcgactggtc gagtctacga tttgcgtacg
    29641 atcacaacta cacacaaagt ggcgttgaaa gatggaggtg caagctaatg tggaaaacga
    29701 ttctgcgacg tgttttattg atgatcccgc aagttattat tttaagtgtt ctgatcttct
    29761 tattagccca agcgatgccg ggggatccat ttaccggttt gatcaatcca aatcaggatc
    29821 cggcagtgat cgaacagatg cgacaagcag ctggcttgaa tgacccatgg tatgtccagt
    29881 acttccgttg gatcggaaat gctttgcaag gcgattttgg tcaaagtttc ttatacaaac
    29941 gctctgttgc tgacttgatc ggcgaacgga tcatcaacac tttatatcta tcgatcttga
    30001 cagttgtcct tacttatttg attgctgtgc ctctggggat gcttgctggc cggtatcaaa
    30061 attcaatctt ggataagatt gtcgtaatct acaacttctt tagttttgct gttcctttat
    30121 ttatcttcgg cttgatcatg ctatttgttt ttggttatcg tttaggctgg ttcccaacaa
    30181 gtggatcaca aacactaggt ttgcaaggtg gatttatcac tcaatggata gaccgtttga
    30241 aatatattct gttaccatca attacccaag catttttggc gactgccgtc acaatccaat
    30301 atctgcgaag tgaagtaatc gattcgaaat cgctcgattt tgtcagaaca gcccgttcca
    30361 aaggggtacc gacgaataaa gtattcagtc gacatatttt ccgaaatgca gcgttgccga
    30421 tggcttctca aatggggtat gaaatcactt cgttgattgc tggttctgta gttatcgaaa
    30481 agatttttgg ttatccggga gtcggaaaac tatttattga ttccatcggc caacgggact
    30541 ataccgtgat cacatcctta gtattgattt taggaatcgc gacgctagtg gggacattac
    30601 tttcagatat catcatgagt atcgttgacc cgcgtattcg gattcaataa aaccaatcag
    30661 aaaggagaat ggatcgatga gtgaaaaaaa caatacagca gttcaagaaa gtattcctcc
    30721 tatggggata cgaatgattg ctcgagaatt taaaaaagat aaaattgcta ttttttcatt
    30781 aggtttgcta gtggttttat tattagtgat ctttatcggt gcgatgctga ccgatcaaga
    30841 caaagtgatg tatgttagta tcttcgacaa atacgctgca ccaggaacag tttcgacaca
    30901 aggacaaaag ttcattctgg gagcagatga aggcggacgt gatgtattag gacaattgat
    30961 cataggtgct agaaactctg ttacgatcgg atttgcgatc acgatcctta cttcgatcat
    31021 cggggttgga ttagggatca tctctggcta ctacggaggg atcattgaca acatcttgat
    31081 gcggatcgtt gattttatca tgattctacc gatcatgctg atcattatcg tcttcgtatc
    31141 gattatttcc agatacaatg tctggtcctt tatattcatc atgagtgcat tttattgggt
    31201 agctaaagcc cgattattcc gaagcaagac attatctgaa gcaagaagag attatgtcag
    31261 tgcctcaaaa acaatgggaa ccaatgattt taaaatcatg ttccgcgaaa tcatgccgaa
    31321 tctaagttca ttaatcatta ctaatttgac gatgaacttt gcggcaaaca tcggtatcga
    31381 gacaactttg actttcttag gtttcggctt gccggcaaat gtacctagtt taggtacatt
    31441 gatcggttat gcaagtaacg gagaagtctt agtgaatcgg caatggatct ggttacccgc
    31501 atcaatcctt attttagttt tgatgttgag tatcaattat gttggacaag cattcaagcg
    31561 ttctgcagat gcgcgccaac ggttaggcta gtatagaagc gaagggggaa ataggaatga
    31621 agaaaacgat ttttggattt gcagcgctga tgtctgctgt cgttcttgca gcatgcgggg
    31681 gcggaggtac agatacctct ggaaacaact ctggaggcaa cggcaataac aaagaggaat
    31741 taacttttcc aattgcaaca aaaaataatg atgatgcaat cgaagacggc gaattagaag
    31801 ctgcggtcgc aacggacagt cagtttaaag gattgttcca gtgggagttt taccaagatt
    31861 catatgatgc agcttttatg gctccttctc atgaaccgct atttgcaaac gatgacgatt
    31921 tcagaatcac agacgacgga gctgcaacgc ttgatctaga tgaagatacg aaaaaagcga
    31981 caatcaaagt aaaagacaac gtcaaatggt cagatggtga agatgtaacg gcggacgatg
    32041 tgattttccc ttacgaaatc attgggaata aagattacac tggtatccgt tatgacgaca
    32101 ctttccgtaa catcgtaggt atggaagaat atcatgatgg aagtgccgac acaatctccg
    32161 gaatcaaaaa aatagacgac aaaacagttg aaatcgaata taaagaagta aacccaagca
    32221 tgcttcaatc gggtggtggt atctggtcat atgcagcacc aaaacatacg ttagaaggtg
    32281 tagcaatcaa agatatggaa tcaagcgatc aagtacgtaa aaatccagtg acttttggcc
    32341 cttattacat gagtaatatc gttgcaggag aatcagtaga atacttgcca aacgagcact
    32401 actggaatgg tacgcctaaa ttgaaaaaga tcacgatgaa atctgtaccg atggcaagtg
    32461 caactgaagc attgaactct aaattatatg atatgatttt ccaaatgcca acagatactt
    32521 acgataccta taaagaaatt gctggctata caaacttagg ccgtgaacaa acttcttata
    32581 cttatctagg attcaagtta ggtaaatgga atgcagatac aaagagtgtg gaatacaatc
    32641 cagattcaaa aatggctgat aaatcattac gtcaagcaat gggctatgca ttagataata
    32701 atgcagtagg tgaacgtttc tatgcaggtt tgagaagcaa tgcgacttca ttgattcctc
    32761 cagtattcca gtcataccat gatgatgaga tgaaaggatt cacacaagat ctagacaaag
    32821 cgaaaaaact attagatgat gctggctaca aagatgtgga tgacgatggt ttaagagaag
    32881 acaaagatgg aaataaacta acaatcaact ttgcttcgat gtccggtggg gaaacagcgc
    32941 aaccattggc agattactat atccaacaat ggaaagaaat tggtttggat gtccaataca
    33001 caacaggtcg tctgatcgaa ttcaattctt tctatgataa attagaaaac gatgatcctg
    33061 aaattgatgt ttaccaagct gcttggaata caggaactga tccaaatcca agtggactat
    33121 ggggaccaaa tgctgcgttc aactacactc gttatgaatc agaagaaaat acaaaattga
    33181 tcaacgatat cagttcaagc aaagcatttg atgaagagta ccaaaaagaa gcatatcaaa
    33241 aatggcaaca atatgcattt gaagaagcat ttgcgattcc aacgttgttc cgtaatgaag
    33301 tattgccagt caacgaccgt gtcaaagact gggattggtc ttatgatgta ccaaacccat
    33361 gggcaaatgt ttctgttaca gcagattcac gttcataatc actaaa
//
LOCUS       DAMEET010000025        31981 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.025, whole genome shotgun sequence.
ACCESSION   DAMEET010000025 DAMEET010000000
VERSION     DAMEET010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 31981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.025"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..379
                     /locus_tag="PB780_001011"
     CDS             <1..379
                     /locus_tag="PB780_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDE0733081.1"
                     /translation="EVDPEEKPDVTPEPDTDARDQGIPEKINKKTIQEDGKKESKKSN
                     LAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTFASPETKNKQLPKSGESQNKVILW
                     SGIILLSIATMLSAKRFKQNRSL"
     gene            complement(505..1584)
                     /locus_tag="PB780_001012"
     CDS             complement(505..1584)
                     /locus_tag="PB780_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDE0733082.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(1693..2133)
                     /locus_tag="PB780_001013"
     CDS             complement(1693..2133)
                     /locus_tag="PB780_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDE0733083.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(2190..2600)
                     /locus_tag="PB780_001014"
     CDS             complement(2190..2600)
                     /locus_tag="PB780_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDE0733084.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(2781..3215)
                     /locus_tag="PB780_001015"
     CDS             complement(2781..3215)
                     /locus_tag="PB780_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDE0733085.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(3315..4163)
                     /locus_tag="PB780_001016"
     CDS             complement(3315..4163)
                     /locus_tag="PB780_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDE0733086.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            4552..6318
                     /locus_tag="PB780_001017"
     CDS             4552..6318
                     /locus_tag="PB780_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HDE0733087.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            6420..7679
                     /locus_tag="PB780_001018"
     CDS             6420..7679
                     /locus_tag="PB780_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDE0733088.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            7826..9127
                     /locus_tag="PB780_001019"
     CDS             7826..9127
                     /locus_tag="PB780_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDE0733089.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            9130..9981
                     /locus_tag="PB780_001020"
     CDS             9130..9981
                     /locus_tag="PB780_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDE0733090.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            9994..10809
                     /locus_tag="PB780_001021"
     CDS             9994..10809
                     /locus_tag="PB780_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HDE0733091.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            10960..12126
                     /locus_tag="PB780_001022"
     CDS             10960..12126
                     /locus_tag="PB780_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDE0733092.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            12356..13003
                     /locus_tag="PB780_001023"
     CDS             12356..13003
                     /locus_tag="PB780_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDE0733093.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            13087..14031
                     /locus_tag="PB780_001024"
     CDS             13087..14031
                     /locus_tag="PB780_001024"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HDE0733094.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            14166..15617
                     /locus_tag="PB780_001025"
     CDS             14166..15617
                     /locus_tag="PB780_001025"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HDE0733095.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            15638..16474
                     /locus_tag="PB780_001026"
     CDS             15638..16474
                     /locus_tag="PB780_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDE0733096.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            16474..17133
                     /locus_tag="PB780_001027"
     CDS             16474..17133
                     /locus_tag="PB780_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDE0733097.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            17259..17948
                     /locus_tag="PB780_001028"
     CDS             17259..17948
                     /locus_tag="PB780_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733098.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            17954..18910
                     /locus_tag="PB780_001029"
     CDS             17954..18910
                     /locus_tag="PB780_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HDE0733099.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            18954..19466
                     /locus_tag="PB780_001030"
     CDS             18954..19466
                     /locus_tag="PB780_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDE0733100.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(19722..20270)
                     /locus_tag="PB780_001031"
     CDS             complement(19722..20270)
                     /locus_tag="PB780_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDE0733101.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(20359..21540)
                     /locus_tag="PB780_001032"
     CDS             complement(20359..21540)
                     /locus_tag="PB780_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDE0733102.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(21776..23284)
                     /locus_tag="PB780_001033"
     CDS             complement(21776..23284)
                     /locus_tag="PB780_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HDE0733103.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            23443..23634
                     /locus_tag="PB780_001034"
     CDS             23443..23634
                     /locus_tag="PB780_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733104.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            23837..24754
                     /locus_tag="PB780_001035"
     CDS             23837..24754
                     /locus_tag="PB780_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HDE0733105.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            24747..25820
                     /locus_tag="PB780_001036"
     CDS             24747..25820
                     /locus_tag="PB780_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733106.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            25968..26486
                     /locus_tag="PB780_001037"
     CDS             25968..26486
                     /locus_tag="PB780_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733107.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            26467..26859
                     /locus_tag="PB780_001038"
     CDS             26467..26859
                     /locus_tag="PB780_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HDE0733108.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            26935..27870
                     /locus_tag="PB780_001039"
     CDS             26935..27870
                     /locus_tag="PB780_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HDE0733109.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            28003..28782
                     /locus_tag="PB780_001040"
     CDS             28003..28782
                     /locus_tag="PB780_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDE0733110.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            28775..29092
                     /locus_tag="PB780_001041"
     CDS             28775..29092
                     /locus_tag="PB780_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDE0733111.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            29336..30946
                     /locus_tag="PB780_001042"
     CDS             29336..30946
                     /locus_tag="PB780_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDE0733112.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            31103..31951
                     /locus_tag="PB780_001043"
     CDS             31103..31951
                     /locus_tag="PB780_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDE0733113.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        10511 a         5693 c         6256 g         9521 t
ORIGIN      
        1 agaggtagat ccagaagaaa aaccagatgt gactccagaa ccagatacgg acgcaagaga
       61 ccaaggaata ccagaaaaaa taaacaaaaa aacaattcaa gaggatggaa aaaaagagag
      121 taaaaaatca aatctagcaa ttctaaaaat aaatgaagag cagctgaata aaaaatcacg
      181 aatatttgat agcgcccaat cagctgaaac cttaaaaagt agcaaggata caacctttgc
      241 tagtcctgaa acgaaaaata aacagctacc aaaatccgga gagtcacaaa acaaagttat
      301 actgtggagc ggaatcattc tattaagtat agcaacaatg ctttctgcaa aaagatttaa
      361 acaaaacaga tctctctaac atttgaataa tgtctgtgaa cgcatttttc aaataaaaag
      421 aaatatccag aaaacgaccc caaaatacca gattgaaatc tgatatttgg ggtcgttcat
      481 actttatctt tcttcatatt tattttaagg gatatctgtt tcttttggaa gatggttcgg
      541 gatctttttc actttcaata catctaaaaa gaaggtaaag atcggaatgc cgacaatcaa
      601 gccccaaacg ccaaagaacc gttcgctaac aagtaagacc acaaatgtat aaaaaatcgg
      661 cagttctgtt ttgctggaca tcaacttcgg attcagcaca taagactcaa ttagatggac
      721 aatcaaaatc gtaatcagga tataaacaac atctcggatg cctccttggg aataagctat
      781 gaaagataga ggaatacaag aaatgataac acctgctact gggatcagac ttaaaatgaa
      841 aatcatgatc gctaaactca gcagctgatg aaagccaaat acagccaagg cgattgtagt
      901 gagcaacgtg tttaaaagag cgataacaaa ttgagcttct aataccaaac caaatgtatt
      961 gacaaactta tcggcaaaat agtaaatatc ttgaaagaac caagaaaatt cactttttaa
     1021 aaacaatctt gagaatacaa ttgttttttt cttttcaatc ataaaaaaga aacttaagat
     1081 gaatgacata gcaaacgcca ctgccatttt tcctatatcc tgtaaatagc caagcgcaat
     1141 cgaagcgcca ttttgaagct gttcaaaaaa atcgtttcgc tcaatataag tatggatata
     1201 ttgtaaaaca ggatctgcat cttttggctg atgctcgtaa aaatcaataa cggactggat
     1261 catatcaaat gtttgatgaa taagcatcgg gatatacttt gtgaatgcca gatagataag
     1321 gtaaacgatc agtgtataaa tcatgatagc taaaacacct gtagggattc ggacaaatcg
     1381 ctgaatgatt tgaacaagat gaatcatcaa ataagtaaag ataaatgtga gcaaaatggt
     1441 tgtaatcata cttctaacta agaaaagaac aaaaacgaca aataataaaa cagtgagacg
     1501 gcgtattttt tcattcgcca aaaaacgctg ataaacggtc aaatcgacaa cctccaaata
     1561 acaaaaaata aacacgttat tcattatatg atggaatatg aaaataagaa agagaaagag
     1621 caaaaagaaa aaccgctaaa tgacgttatc ttatgagttg gttttcctca taaaatcgtt
     1681 catctagcag tttcagccct ttttctggtc ttgatgaaaa ttatctagca tttttcgggt
     1741 cagatgttgc agtgtatcca attcttcctg ttcaagtgga agataagact tgatcttttg
     1801 tggaatacct tctgcttgtt tttgcaacgc agcaccttga ggagtcagcg taacatttac
     1861 gattctttca tcacccgttt cacgctgacg attaatataa tttttttctg ctaacttttt
     1921 tagtaaggga gtcagcgtac cagaatccag acacaaatca tttcctagct ctttcactga
     1981 agtctttttc ttttcccata aaactaacat cacaagatac tgggtatagg taagattcag
     2041 cggcttgagg agggaagtgt agtgtctaat gatttcctta gaggcggcat ataaaggaaa
     2101 acatacttga ttaactaaat atagatcttc catttttcca cctccgctgt tttactatag
     2161 tgtatcatag cggatttaca caaaaaagtc tattcactaa ctgctttcag agtaacttct
     2221 atatttccag ctgtagcttt tgaatacgga cacactttat gcgctgcctc taataattct
     2281 tgcgtttctt ccagagaaag accttcgata gatccttcaa tctctacacc taagacaaag
     2341 ctcgtttcac tttctgaata aagggaaact tgtgcactga cagtagaagc cccagagata
     2401 tttttttgct gtttgatcaa ttctaatgca ctgttaaaac atgcactgaa accagctgca
     2461 aacaattgtt cagggttggt tgcattttcg acgcgtttac caggagctgc aatattcaat
     2521 tcgaatgtac gatcgggatt atgaacttct ccagagcgtc cgcctgtgtt gatcattttc
     2581 gtagaataca tttttttcat aaaattacca cctttaatta tattttatac aattgaattt
     2641 tacacaatat aattgtggaa agcaaagaaa tggattggca taagataaat aagagaaagc
     2701 acatgaaaca gacgcgcttt ctcgaatagc aaacatatac aaaaaaatat cagagacaaa
     2761 aactaatctc atcataaaca ttacatatat ttctcaaaaa gttgttttct ttcttcaaaa
     2821 cttacttcac gatggacctg atgaagcatc cataaataac caaaaggatc tctgaacatt
     2881 gagttagtca caccaaagtc catcatttct gtcatttctt gaatctctac acagccattt
     2941 cgtaatgctt tattatgtgt gtcctcgata tcagagacga tgatattgaa ccagatcgta
     3001 tttggatgtt cagcagtagg agcagtcaaa ccaagagacg tgttttcatc cagcatatgg
     3061 aatctcgtgc catacaaggt aaaaatgact tcattattgc ctctagggta atctgttgcc
     3121 tcaatcagtt gtatttcatc aaagattgtt tgatacagtt ctagtgcttt caaactatca
     3181 tttaatacaa aatctatttc cgttccttta tgcataaaga tctcctctcc ttttcagtcg
     3241 aacagttact agattctttt attataccag ttaaagaaaa cgcattcaaa aaaatagcgg
     3301 atttacttac caactcattc gattggatga taagtaaatc cgcgtcccat tacttctgct
     3361 acgctgctga caatgacaaa ggcacttgga tcgatttctt ctatcaatgg tttcattcgg
     3421 gaaaattcat gatcagtcac aacgatcaat aaaacctctt ttgactttcg attatacccg
     3481 ccttttgcct ctagcagggt aaggccacgt ccaatctcag cacttaagcg aaggcggatg
     3541 tcttccaaat aatgttcact catgaccata acagatttct ttcgattgat acctgtttcg
     3601 atataagtca tgacaattga agtgattgca atagataaaa tagcataaag aaactcttct
     3661 attccaaaaa caaacagatt aagagaaaca acgaatgcgt ctgtaaaaaa taagccaatc
     3721 gaagtattca agtggaaata ttttttaaaa atgaggggag ggatcgtcgt accgccgcta
     3781 gaagagttgt ttttatataa aatcgcaact cctaaagcaa aaatcgcact tccaaaaatg
     3841 acagacaaca accgatctga agtaatcatt gtttctggaa ctattgcaat gattaaagga
     3901 aaaaacaagc ttccgaaaag tactttaaaa aagggttttt tacctaaaaa tagaagagct
     3961 agtaccaaca taacgatatt caacactaaa ataactatag ctcggtcaaa tcctaaaaca
     4021 gcttccagta atattcctag accgctgaca cctccagcag caatatgatg aggccctaaa
     4081 aacatattga tagcgactcc taatatgaag agggcaaaaa taatcttaat gtatgtccat
     4141 ataaattgtc tcgtttcttt catttatcca cttccttaat tcattaataa tactcttatc
     4201 ttattataac ttacttttca ccttaagtta actttaatta ataaaaaaat taggctagtc
     4261 taaattatta tgtttcattc ttctagaata tacttctacg ttaaaaactg ttaaaaaact
     4321 tttactttag aaaattggag aaattgatcg cttaagataa attcaggaca ctaaatagta
     4381 caggaagtag attaaaactt ttaaaagttt taagtctatc tatttggcaa gtttaaaaaa
     4441 taaaaattcg gtatcaaaaa actgttaccg ataacatttt tgaaagcgtt gtcttttgtt
     4501 tctctatcac ttatagtgat gacgtcatat taaaacacaa ggaggaaatc aatggataca
     4561 gctgcaattt atcatcgacc ggaaagtgaa tatgcatttc tatatacgaa tacaagattt
     4621 cgtattcggc ttcgtactag gaaaaatgat atcaaaaaag tatatgtgct ttgtggagat
     4681 ccatatacca taacaactga aaaatggtat caaaaacaac gaccaatgaa aaaatgtcta
     4741 agtaccaatg ttcatgatta ttgggaaatt gaagtgacga gcgaaacaag gcgcttacag
     4801 tatgcgttcc atgttgttgg ggaagataac acggactgtt tttacggtga tcaaggaatt
     4861 tttccttatc gagaaaatgt gattactgaa ccaaattttt attttcgaat accttatttt
     4921 catcaaatcg atcgattcac tattccagaa tgggtaaaag aaacagtatg gtatcaaatt
     4981 tttcccgaaa gatttgcaaa cggggacaaa gcaaatgacc ccgaacacac tttaccttgg
     5041 ggaagtaaag atccagaccg agaagacttc ttcgggggag atttgcaagg tgtgagggat
     5101 catttggatt acttgacgga ccttggcgtc aatggcatct atttttgtcc catatttaaa
     5161 gccacatcca atcataaata cgatacgatt gattattatg aggtggatcc cgcttttggc
     5221 gataaaaaac ttttaaaaaa cttgattgat gaagcacata aaagagggat acgtatcatg
     5281 ttcgatgctg tattcaatca tatgggtgtc cattcaccac agtggcaaga cgttctcaaa
     5341 aacggagaaa aatcgatcta taaagactgg ttccacatcc gttttttccc agtagattct
     5401 tatcagatga ctgacttacc agaaactgct gaaaatatcc catatgatac ttttgctttt
     5461 actccattca tgccgaaact aaatacagca aatccagaag tgcaaaaata tttgctggat
     5521 atcgcaactt attggataaa agaattcgat attgatggat ggcgtcttga tgttgctaat
     5581 gaagtcgatc atcatttttg gaaaaaattc agagaagcag ttacagaaat caaaccagac
     5641 atctatatcc ttggagagat ttggcattcc tctcaagctt ggctgcaagg ggatgaattc
     5701 catgcagtga tgaattatgc attcacagat tcgattaaag attattttgc aaaaaagaaa
     5761 atcacagctt cacaaatggt cagtggaatg aatcatcagc aaatgcttta cagagatcag
     5821 gtgaacgaag gaacattcaa cttattagat tctcatgata ctgccaggat cttgaccctt
     5881 tgccaaggta acaaagaatt gatgaagtct gtgatggcct ttatgtttct tcaaaaaggg
     5941 tctccttgta tctattacgg gacagaaata gggatgactg gtgaagatga cccggattgc
     6001 cgcaaatgta tgatatggga gaaggaggaa caagatctag agctattcgg ttttataaaa
     6061 gaacttgttt ctctaaggaa acagttgtct aagattattt ctgaaggttc tactcagtgg
     6121 ctaatagtat atgatcgtga agataagctc tatttcacac gggaactaga gggacagatc
     6181 atctatgttt actttaatca gtcaaaagaa ccttgggtag tagaacagga aaatgaggtg
     6241 attcttagtc aaaattgtca gttgttagaa gacgggaaag cagagatacg acaatacgga
     6301 tttttgatca tgtgttgaaa actgttaccg gtaacaacaa gaaagcgttt gcaaatgaag
     6361 atttgtgccc attataataa gggtgtaaca gcacgttgaa tacacaggag gaaatgaaga
     6421 tgaaaaaaag aagtgtgaaa cagttgggtt tagggttaat ctctttagga atcttaggcg
     6481 gattagcggc ttgtggcaat ggtaactctt cagcagaagg ggaagccgat gaaggaaaaa
     6541 cgttaacagt ttccgttgat tctggatata aagattatat aaacgaaata aaaaacactt
     6601 tcgaaaaaga aaacgatgtg aaaatcaagc tagtagaaaa ggacatgttc gatcagttgg
     6661 aatcattagc gttagatggg cctgctggaa agggacctga cgttatgatg gcagcatatg
     6721 accggatcgg tgcgttagga cagcaaggac atctggcgga agtaaaactg ggaaatgaag
     6781 cagcttacga tgaaacagac aaagcccaag tgacttatga tggaaaaatc tacggagaac
     6841 ctgcagtaat cgagaccctt gttttgtact ataacaaaga tttagtcgat acagcaccat
     6901 caacgtttaa agatttggag aatctatcca aagacagtcg ttttgctttt gagtcagaag
     6961 caggaaaaaa tacaggattt ttagctaaat ggactgactt ttattattcc tatggtttaa
     7021 ttgcaggtta cggtggctat gtatttggtg atgatggcac aaatccttca gatatcggtt
     7081 tgaataatgc aggtgctgta gaaggaatct cttatgcaac agattggttc aaaaatgttt
     7141 ggccaaaggg aatgcaagac ataaaaagtg caggagactt cgccagccag caattcatgt
     7201 ccaataaaac tgcagcaatc attgacggtc catggcaagc gcaaacatac aaagaaaaca
     7261 atatcaatta tggtgtagcc aaaattccta ctctaaataa tggacagcct tatcaaccgt
     7321 ttggcggagg aaaaggatgg gtagtcagca attatgctaa aaacaaagac ctctctcaaa
     7381 aatggcttga ttatgtaacg aatcaggaaa atcaagaaaa attctttgaa atggtaaatg
     7441 aaatccctgc aaaccaacaa gctagggaaa cagcaaaggc taaaaacgat gaattaacaa
     7501 cagctgtgat tgaacaatat gaaacagctc aagcgatgcc aaacatccca gaaatgggcg
     7561 aagtttggac aggtgcagaa aacttgatgt tcgatgcagc atcaggttca aaaacaccaa
     7621 aagaatccgc agatgaagca gtaaaaacaa tcagtgaagc catagaacaa aaatactgat
     7681 taaaatgata accgtccatt tttataaaaa atggaacagg tgcttcagat aaaagtggca
     7741 agagcgagat ggaaaattat cttgctcttg ttgccattat cttaaaagca gtttgcggtt
     7801 tagcaagttt agagggggca ttcagatgca gcaagataaa acaagcaata tcaacgaaaa
     7861 aaaaatcacg ctttgttcac ttattccagg gatggggcag tttgtaaatg gccaagcttt
     7921 taaaggtatc gtttttttcg ccatattcgt cctattcatt ggtcagatgc ttctgggcgg
     7981 atttcatgca ttggtaggat tggttacttt gggaagtata ccaatggaag atcattcatt
     8041 atttattttg attgaaggca ctttgcaatt catcattaca atcattttct tgatttttta
     8101 cggtttgaat cttttagacg cttatcgagt agcgaagatg cgtcaagagg gcaaaaaaat
     8161 caatcggacg atgaaggaag tcatacataa tataggtgat gaaggttttc cttatctatt
     8221 gacacttccc gcttatcttc tcatgatttt tacgatcatt ttcccagtcc tagttacatt
     8281 gttcactgca tttactaatt atgacttcaa tcatattcca ccagccagtt taattgattg
     8341 ggtaggggct aaaaactttt ttagtatctt ctttttaagc tcatatcgta atactttttt
     8401 tgcagtcttt tcgtggaccg tgatttggac tttatgtgca acaactttgc aaatcaccct
     8461 tggtattctc acagcggttg ttgctcacca gaaatttatt aaattcaaac gtattttcgg
     8521 tatcattttc ttattaccat gggcggtccc agcttttatt actatcatga gtttttcgaa
     8581 tatattcaac gatagcgtag gtgccatcaa tactcaagtg attcctttca taaaccagct
     8641 tccttttgta gatatcagtg caatctcttg gaaaacggac ccgaactgga cgaaagtagc
     8701 aattatctta gtgcagggct ggttaggatt tccctatatc tatgtgatgg ttacaggaat
     8761 cttacaatcg atttcagaag acttatatga agcagcaaaa atcgacggag caaatgcatg
     8821 gcagcgcttt tcaaacatca cgcttcctgc tgtcttccta gtggcagcgc caacatttat
     8881 tacacagtat acggggaatt tcaataattt ctccatgatc tatctgttta acaatggtgg
     8941 tcccggaagc gtaggcggag gagctggttc tactgatatt cttatttcct ggatctataa
     9001 acttacgact ggaacttctc cgcagtattc tatggcttca gcaatcacgt tgattatctc
     9061 cgtcatcgtt atctgtgtgt cattgctggt atttaaaaag acaaatgcgt tcaatatggg
     9121 ggattaacaa tgaaaaaatt tcattcgagt gctcgattcc aaaaacgact ttctcacttt
     9181 tttacttact tttttctttt aatactgtca gtcattatca tctatccgct tttgatcaca
     9241 gcaagtactg ccttcaagtc aggaaatatt gcagctttta gtttagatct ttcaagtgac
     9301 tggactttag gaaactttac tcggctattc agtgaaacac tttacgggac ttggtataaa
     9361 aatacattga tcatttctgt cctaacaatg atcgtacaag tgactgtagt aactttggcg
     9421 ggctatactt atagtcgcta tcgttttatc ggccgcaaat caagtttgat gttcttttta
     9481 gtgattcaga tggtaccgac tatggcagca ttgactgcct tttatgtcat ggctttctta
     9541 ttagaagcgc tcgataagta ctggttcttg acaatgatct atataggcgg tggaattcca
     9601 atgaatactt ggctgatgaa aggatacttt gacactgtac caatagattt agatgaatct
     9661 gctaaacttg atggggcagg gcatttcagg atttttttcc agattgtatt accgcttgtc
     9721 aaaccgatgc tcgccgttca agcattatgg gcttttatgg gaccttttgg ggatttcatg
     9781 ttagctaagt tcctgcttcg aacacctgaa aatcagactg ttgctgtcgg cttacaaaca
     9841 tttatttctg atgtgacaaa ccaaagagtc tctctttttg ctgctggggc aattttgatt
     9901 gcactaccga tttgtctatt attcttcttt ttacaaaaga actttgttac cggcctgaca
     9961 tctggcggaa caaaaggata ggagcagagt atcatgcaaa catttccgtt aaactatttt
    10021 tccgcattac gtacgccaac tcaagtgttt gcgggacgca aactattatc atggcctaaa
    10081 tttttcttga tatttgtatt tttagtttcc ttgatggtca tgcctgtgac tcttttttat
    10141 gccaatcaga tccaagcgat tccattagaa caatttctat ccgttcattc gctgatcgat
    10201 gaacaaggaa cccaaaaatt ttctgaactt gaattatctg aaactgggtt acaagcatcg
    10261 caacaaacga tagctgtgac ccctgaaatt ttggtaggtg tgtctttatc tgagaaacaa
    10321 caatccgatc atggaacttt tattgatttt gagaaggaac aatgggtgat ccaacaaaag
    10381 gataaatctg gtatacgccg ctatacgatg aattatagcc catctttcca accagattct
    10441 gtacgtacgc ccgaagattt ccaacgattt ttagaaagag aattttatgc cagtaatcgt
    10501 ccaacaatca ttttatctta tagtttaagt cttggtcttg tcctatttgt catgacgagt
    10561 ctcattttat tcggggcttc ctttttcttg tggatgacaa gaaaaagcca actttcatcc
    10621 attcatacat tcaaagagag tgcaaatttg atgctgaatg ttatgggaat aggttccatg
    10681 gcagcagcag ttgtagggtt tatccacttt gactttatct tgatgctaag tgtccaaaca
    10741 acgataactg tcttgctgct tttatgggtt tttgtcaaaa caagattcaa agataagcgt
    10801 gtcgaatgac aagtttaagc gatttatgcg acaatgaatg tgaaaaaaat gaaaaataaa
    10861 gtagtttttg aaaaatttct gaaaaaatta aacttcttag ttgattcttt tcttttttag
    10921 tattaacgta atagggtagt ttttttaagg aggaaaatca tgttagagaa aactttatac
    10981 aatcaattat tttcacgttc tttctcgctc cctgtcgaag taaattattg ggacggtaca
    11041 acgaaacaat acggagatac ggacactcct ccccaaatca aaatcaatat ccatgaagaa
    11101 atcccaatga aagagattac aagtaacgct tctttggcac taggagaagc ctatatggat
    11161 catcgaatcg agatcgaggg aagtatccaa gcattgatca acgatgctta tcagcaagca
    11221 aacagctttt tacgaggaaa agattatcta aaatggctgc caaagaaaga aaaacatacg
    11281 aaacaaaaaa acaaagaaga tatccatgcg cattatgact taggtaacga cttttataaa
    11341 ctttggctag atccaacttt gacttattct tgtgcttatt tcaatacacc tgaagatacg
    11401 ttggaacaag cacaaatcaa taaagtccat catatcttag acaaactctt tatcaaagaa
    11461 ggcgaaacgt tgcttgatat cggttgtggc tggggaacat tgatgttcac tgctaccaaa
    11521 gagtataatg tcaaggcaac tgggatcaca ctaagtgaag aacaatacga tttgattacc
    11581 aaaagaataa aagaagaaca tttagaagat aaatgccgtg tattattaat ggattaccgc
    11641 gaactaaaag gcgaaacgtt tgatcatatc acaagtgtag gtatgttcga acatgtaggt
    11701 tctgaaaatc ttgaaggtta tttcaaagtg gtaaaagatt tattgaaacc aaaaggaaca
    11761 gctttgatcc atggaatcag ccgacaacaa ggcggtgcga caaatgcttg gatcaataaa
    11821 tatattttcc ctggcggtta tgtccctggt gttactgaat tagttgataa tattacaaaa
    11881 aacgatttgc aactgattga tttggaaagc ttacgtcgtg actaccaatt gacattagaa
    11941 cattggacaa agaattttca tcaagtccaa gatcaagtca cgaaagaaat gggtgattcc
    12001 ttctatcgaa tgtgggatct atatttacaa gcttgtgcag cttcatttga atcaagtaat
    12061 atcgatgtga ttcaatattt gcttgttcaa cctggaaaca atgacatccc aatgcatcgt
    12121 gcataattga ggcaaaagta ctgtttaaag actatttaaa aagaagctaa gttttatgat
    12181 gctccagaag tgaacttttt tgtctaactt ctaggattgt ctctaaagct tagtttcttt
    12241 ttctttatat tttcataata aaagtttggt tctttaattt ttaaacatga cagaaaattt
    12301 tttcttcgtt atacttgtaa tagaatacta ttttataaca aaggggatcg aatcaatgaa
    12361 aaaaactgtt atttatacta gtttatttgc agctttgttt ttagctgctt gtcaatcaaa
    12421 taccaatcaa ggcacatctg ggaatacgtc ttccagtagc gaacagcgct cttcacaggt
    12481 atcggactcc tcaacaaaag caagcacatc ttcgtctagc cagacaaaaa gttctagtga
    12541 agcaagtact cagtcagata tcaaagtgtc tgcagctcgt gcgatcgaat tgttccaaga
    12601 taaataccca gatactgcaa ttactagttt ggaattagat agtgattggg gatcttactt
    12661 ctacaagata gaaggtgtcg atgatcaaaa agaatacgaa gcaagaattg atgctcaaaa
    12721 tgaaaaaatg gagacagaga atccagaaac tctagatcga gatgaacaaa atggtcaaaa
    12781 gaaaaaagaa gatggtctgg atgttgataa attgatcacg attgaggaag cagcaaaaat
    12841 cgccgtcgac aaagtaggaa gcggtacggc aacagattgg gatttggata aagatttagg
    12901 tactacttat tgggaagtca aagtaaaaga cggcaatcaa agtacatcag tcaagatcaa
    12961 tagccaaaca ggcgaagtac tgtcaactga acaagatgat taagaatttc ttcggaaaac
    13021 aagagttgat ttttctttta gtcatgatac actagacaaa ataacttgtt tgaaggagcg
    13081 atttacttga gtttagaaac gacctttgcc aatcatatct ttgccaatcc tttgatgaat
    13141 gcatctggtg tccactgcat gactacccag gaattagatg aactggctca ttcagaagca
    13201 ggagcattta tcactaagag ctgtacgatc aatgaacgaa aaggaaatcc cgaaccacgc
    13261 tattttgatg ttcctttagg gagtatcaat tctatgggac ttccaaactt aggtttttct
    13321 tattatctag agtatgctct tgcgtatgaa aaagtgcagg aaaaccaaaa tcaaccgtta
    13381 tttttttcca ttgccgggat gagtgtccag gaaaacttgg aaatgcttga gaaaatagaa
    13441 aaaagtgggt ttaatgggat cactgaatta aatttatctt gtcccaatgt gcctggaaaa
    13501 ccacaactag cttacgattt tgaggccact tatgaaacac tgaaagaagt attcagcatc
    13561 ttttctaagc cgctaggaat caaattgcct ccttattttg attttgctca ttttgatcag
    13621 atggctgata tcttgaatca gtttccattg acctatgtca atgctatcaa cagtgtgggc
    13681 aatggtttgt atatcgatac agaacaagaa gcagtagtca tcaaaccaaa agaagggttc
    13741 ggcggcattg gcggagagta tatcaagccg actgctttgg cgaatgttcg cgctttttat
    13801 acacgtttga aaccagaaat ccaaatcatt ggtacaggtg ggattcggac aggacaagat
    13861 gcatttgaac atctgctatg tggcgcaagc atgttacaga ttggtacaga gcttcataaa
    13921 gaagggccgg aaattttttc tagaatcata aaagaattaa cgcagattat gtcagaaaaa
    13981 ggatatacgt caattgacga atttaaaggc aaattacgca ccatttcata gcaaagagac
    14041 gaaggtcatg attgatcttc gtcttttttt ataaaaaaca ctcgtctgtt cattcctgtg
    14101 caagcgtact cattgtatgt tagacgaact tgtgggaaaa taacagaggc aaaaaggagg
    14161 ataatatgaa tgatgcaaca gaattagcaa aagagcttaa agaaaaagaa tttagttttc
    14221 aagaactagt tacggatata cagaaaaaaa taaagaagca aaatcatgaa ttgaatgcgt
    14281 ttgtcgcgct tgaaccattc gagagtaatt cccacgagct attgattccc cagtcagaaa
    14341 aagggccatt ggctggtatc ccatttcctt taaaaatgct cggtcaagaa aaaaagggat
    14401 ggctagctac ttcaggatct cgtttatttg aaacacaccg agcttctcat acatctaatt
    14461 atgtgcggca agcagaagcg ataggtctag ttccttttgg acaaaccaat gcgccagagt
    14521 ttggtttcaa aaatattaca gacccagtca tctatggccc agcaagaaac ccttggaacc
    14581 ttgaatgtac accgggcgga tcaagtggag gagctgctgc tgcagtttca tcaggaatcg
    14641 ttcctttagc tggtgctagt gatggcggtg gttctattag aatacccgct tcgttttgcg
    14701 gattgattgg gctgaaacct tcgcgaggga cgatgccagt tggtccatat gcttggagag
    14761 gatggcaagg agctgctatt gatttcggct tgacagtttc tatgagagac acggaggctt
    14821 tattttatgg gatgagaagt atccattccg gtgcgccata tcaagtatcg ccagttgagt
    14881 ggcaaacaca tccaagaaaa aaacggttga agatagcttt ttgtgttgat tcaccgatcc
    14941 actcacagat tagtgaagag gcagtacttg ctgtaaaaaa agctgtagat ttccttggaa
    15001 aagaagggca tgatttgaca gagattcctt acccactaga tggtcgtaaa ctgatcacgt
    15061 cttattatca gatgaatggc gcagaaacag cagcaatgat ccgctcgatt gaaacaggca
    15121 tccagcgagc tgtcagaaaa gaagaacttg aacccatttc ttggactttg ttccaatatg
    15181 gtcagaagct ctctgccgct acttacatcg attctctgca tgtatgggat gaagcagcaa
    15241 ttgtcatgga aaacctacat caagaatacg atctattcct ttcaccaacg actgcattta
    15301 ccgcccctaa aatcagcgaa gatttgcaaa gtgatgcgat aagagcacag atggaacaca
    15361 tcagtgattg ttctgaagta gaaggtttag acgttgtttc ttccatgttt gaggagagct
    15421 tgaagatcac accttataca cagttggcta atctgactgg acaaccagca atcagcttgc
    15481 ctactcattt gacaaaagaa aacatgcctc taggaatcca atttatggct gccagaggaa
    15541 gagaagattt gctatttcaa atcggctatc tatttgaagc aaatcatcag ttccatttac
    15601 caaatccatg gaaatagcag aaacaaggag aagaagaatg aagaaaaaaa gattgtttgc
    15661 tctaataact agtgcctttt ttattttaag cgcttgcggt acagctgccg ctaatcagga
    15721 agataacagc tggagtaatg tagaaaaaaa gaaagaatta gtagtagcga cttctggcac
    15781 actgtttccc gcatcttatt ataatgatca aaatgaatta gttggttatg atgtagattt
    15841 ggcaaaagag gtggctaaac ggttaaatgt caaaatctcc tttaaagaat acaacgtgga
    15901 tggacaaatc acttctgttt caaaaagaga atcagatttt gctgcgaacg attttggtat
    15961 cacaaaagaa agagaaaaaa aattctccct ttcagagact ttcaaatatt catttgacag
    16021 tatgatcgta cgaaaaaaag atgattcagg aatccactca cttgaagatc ttaaagggaa
    16081 aaaagcagct ggcgaaccca atactagcta tatgcgttta gcggaaaaac acggagctaa
    16141 attagtcaca tatgacaatg ccacgaatga tcagtattta agcgatgtag caaatggtcg
    16201 aacagatgtt attctaaatg attattatct gcagaaaatg gccgtatctg ctttaccaga
    16261 tatccctgta aaaatactgg agaacgttta tttcaatgaa aacgaaagtg gttttttatt
    16321 gaaagaagga aatgatgctc tgaaaaagaa aatcgatggc gtattaaaag agatgaaaga
    16381 agatgggacg acaaagaaaa tatctgaaaa atattttcaa actgatgttt cagtcaaacc
    16441 gaaagaaacg atggctgagt cggtgagtga gtgatgtact ttcctctaat tgatttttcc
    16501 ctcatgtgga cttcacttcc tttcgtactg caaggcttgg tatacacatt agggattggc
    16561 ttcgtgtcgt ttcttttagg aaatttggta gggttgcttt tgacagtttt aggtcttttg
    16621 gattggctgc ctctgaatgt atttattcgt ttttatttat cgttctttag aggcattccc
    16681 gcactagtcc tgctgtttct gttatatttc gggttacctt atcaattaag tgccttaacg
    16741 gcttcagtca tctgctttac gattaccagc agtgcattta ttggtgaaat ctatcgagga
    16801 tcactagcag gcgtttcttc aggtcagtgg gacgctggtt tagcttctgg tttttctttc
    16861 tttggggtat tgaaatatat cgttttgccg caagcttttc gaatctcagt accggcactc
    16921 agtaacgtag ctatggatct agtgaaaggt acttcgttag cagcaacaat cacagtgcca
    16981 gaaatatttc aaaaagcaaa gatcataggc ggtcggacat tcgattattt ttctctctat
    17041 attttagttg cactgattta ttgggtcatt tgtatcggga tcgaaaaagt gcaacagtat
    17101 ttagaaaaac gttctatcca tcagtcaatc tgaaaaatag aaatggtgct agcgtggctg
    17161 gcaccatttc tattttttgt ttcttgtata ttctgcctag tattttaaaa ccatttcatg
    17221 taaaatgtaa atcgaaaata tgttcggagg tagaaaacat gaagaccccg ctaaccatcg
    17281 aaatagaatc ctcgttatac caatattgca tcgagcaagg cggcttggtt gtagaagaag
    17341 tcacgatgcc tgatgaccaa ggaattgtag atacgcttag ctgcttgaca aaatttgatg
    17401 gaagccgaga atggcgatgc tatgagctga aagtgacaaa agcagatttt cgctctacag
    17461 ccaaactgtc atttgttgga cattataatt attttgtttt accaagtaaa ctatatgaag
    17521 aagtaaaaac tgaaatccct acaggaatag gtgttttagt gtatcgtccg tacacggttg
    17581 aagaggagat gcctgcctct ggtacatttc tgattgcgaa aaaagcagca aagaaagagc
    17641 ttgctgtacc ggaagaagct ttgacaaacc gcttcatggc ttctttattc agggaagtaa
    17701 gaaaagcaaa gcaaatggag cgaggcgtaa aatattttcc aaaagatcaa ttgtacaaag
    17761 aattgaaaag acgttcagaa aatcaagatc cattttctaa cgaacgatat tatgaacggt
    17821 ttttagagga aatagctagt caggcaatca atgagttgca agaagaagtt atcgcgcttc
    17881 agcatgatta tgaatatttg aggcaacaac agcagatccg tcggttgcct acagagccat
    17941 tggagtgaaa aatatgtact atccttattt aagaggaaaa caatttgacc tattagcatt
    18001 aaaagaagca ctgtcgcgag gattgctctc caataaaatc caaccagtga tcgaaccagt
    18061 acgtgattcc gctacgctta aaaatgtgat cgaattattt caaaaaaagc accatccaat
    18121 cgctgtgatc caaaatcccc aagtaggaca attcaaatta ttcgatcagc atgtgcacag
    18181 ttggacagtc aaagaaaata gctctgtcgt ccctgcacag atcttaacgc cagaaaatct
    18241 cagtgaagta ctggattcac ctccagcgtt tcttgttttc gatggacagc actacccaaa
    18301 agatacagag gtgtggaaac agttggcagg agttgattcg aaatttttga taccagatac
    18361 tagtcgcttt cgcatctggc tgcccgaaaa caaaattgtg atccgtgact cttttcaaac
    18421 gagaaagcat gttgaaagtt atgcagataa aacagatgac ttttttagtg atgactatct
    18481 ttttttccac gcagacggat atattggatt ttctgatttc acgatcgaag gtagccgtta
    18541 tttcgataaa gggttcccta gccgagcgct tgctatccat ctaacgtata ttgatgctta
    18601 cggcaatata cgcgtgaaac attttgtctc tgacagtaac gactctgcta aagaccaagc
    18661 attgaagttt cttgaagcag gagataaaat gaaaaaatgg atcatgcgtc accatcatca
    18721 actcttgatt acttcaggaa tgattgaatt attggctctt taccagcaac aaaaattccc
    18781 tggactggga gtgttgaaaa agtggtcact tatgcatcat ttggaactta ttagtcacct
    18841 gctagatcat ccaaaagatt ggcttaaata ttattcaaaa gttcaggaac tatatgaagt
    18901 gatccaatga ttagataatg taaatgaaaa aggaatagaa aagaggaaac tggatgtata
    18961 ttcggcaaac aaggaaagaa gaagtagaag aagtggtaga aatcattaat gccgcagcgg
    19021 atttcattgc ttcgcaaggc tctcctcaat ggcaaaacgg gcagaaacca acaaaagaaa
    19081 cgattttgaa cgatattgaa agaaaagaaa gctatgtatt aatagtggaa gatcaaattg
    19141 ctggaacagc tgcattagtc tcaggcgtgg atcccgttta cacaaaaata gatggtagtt
    19201 ggaaagaatc aactggtcct tatttatcga ttcatcgtgt cgctttagca gaaagtactc
    19261 gaggaaaaaa actagcaaaa ccgttcttag aacatttgtt gacgattgca gccagcaaaa
    19321 atatccaaga tatccgtatc gatacttatc cagctaatga accgatggaa aagaccatct
    19381 attctgctgg attcacttat caagggatga ttgaatttcc ttttgaacac ggcgaaagaa
    19441 aagcatatca aataatcatc aattgactat aaaatcatct gagcaaagat aaagacatta
    19501 acactactga aaaaaacaat catatgatag ccaagaggtt gtaacagaag tggccagctt
    19561 caaaaaacaa gaaagaattt ccgaaaatgc ttctcaaatt tttgtggaat tttagcgtat
    19621 tttacgaaat agctgctttc tgaaactccc ttcatcgaga ggtcgtgaca tagcttttgt
    19681 cacgatctct cggcttatcc tacgagtatt attcgataca tttatatcaa taatcctttt
    19741 tttattttat ctgcagcttc ttggaacgct ccatcgtttg acacgactaa aagtgagggg
    19801 atagctttca aacgtttcaa ctgtgctaaa tcttctacat aaaccgccag tctggctgaa
    19861 atctgttcag atgaatctcc acgttttttc aggcgcgatt gcaatgtttc tttagagatc
    19921 gtaataaaga taggacggac taccgtcccg aaatactcat ataaatgcca aaaaccattt
    19981 gctgtcaaaa cggcgtaaca atttcctact gtcagtttct ttttaatttc tgctttactg
    20041 gaaccatatt tatgttgagc atattggtcc cactctgcaa actcatttcc cttgatcatc
    20101 tgattgaatg tattgtcaga aacaaaataa taatcctctt gatctttttc cccaggtctt
    20161 ggtgatcgat tcgtgtaagt aatgatcttt tgttccggct gaaaaatagc ttctgcgagt
    20221 gaagtttttc cagaaccaga aggaccgata aatacatagc aataattcat tgtcatttct
    20281 cctttgatga ttgaacaagt gaaaaagctt tggaaaatat ttccaaagct tttaaaagta
    20341 actttgcttt tttgtatttt ataccagcaa gccagtcaca atagcactta aaaagctgac
    20401 taaagtcgct gttagcaaaa ttttcaatcc gtgttttgcg acaagatttc ctttttcttc
    20461 atttaatccc ttcatcgcac cagaaatgat cccgatagaa gagaagttgg caaatgaaac
    20521 gaggaagaca gatataattg ctgttgtacg ctcagtaaaa tggaaattac catttgctaa
    20581 ttctgtcata gcaacaaatt catttgtaac cagtttagtc gccatgatac taccggcatc
    20641 cactgcttct ttccatggga ttccgctgat gaaggcaaga ggggcaaaga tgtaacctag
    20701 gatctcttgg aaagtgatgc cgaatatccc gtggaagata gcattgatca tggcaatcaa
    20761 agcaacaaaa ccgataagca tagcagcaac cgtgatcgcc acatggaaac catcaagaat
    20821 gtattctccc aacatttgga aaaatgtctg ttctttgttt tcttcaatga tcagctcatc
    20881 ttctttttct tctaatacat agggatttac aattgaagcc agaatataac cgccaaataa
    20941 gttcaaaaca agggcagtga tcacatattt agaatcgatc agtgccatat aagatccgac
    21001 gatcgacata gaaactgtag acatagccga tacactcatg gtgaacaaac gtttctcaga
    21061 taggaagggt aattcctttt ttatggaaat aaaaacttcc gattgtccta aaatagcaga
    21121 agctactcca ttataagatt ccaatttgcc catcccgttg attttactta atcctagacc
    21181 aacagctttc ataaataatg gaagaatctt gatatagcgt agaataccga taatagcaga
    21241 aataaataca attggcatca atacggaaag aaagaaaggt gcacttcctt tatttgctac
    21301 accggcaaaa acaaagtcaa caccttgccc agcaaaagca agaagatgat caaacatttt
    21361 tgccaataca gaaacaaccg ctgtaccaaa agttgtacgt aataatacga aaccaagaac
    21421 aagctgtaaa acgaacatca caattagtgg tttcaatttg attgcttttt tgttactgct
    21481 gatcaaaaaa gcaagcccta atgtgacaaa taaaccaata atacctaata aataactcaa
    21541 ataatcactc ctattccaaa ttccgtttta ttttacctgt tttgcaaata aaatactaat
    21601 ctttagtgaa attttcatta cttttttaga gtgaaacgca ttcattttcc attagaaaat
    21661 aaagcgaaca aaataaaagg tttaaaaagt gtaacgtctt agtaaagtct ataaaaaaac
    21721 gaaactatcg attaagctaa tagtttcgtt ttcaacattt ataagtaaac tttttctatt
    21781 ggtaatatag attttcggaa gggtagacag gtgcgcaggt aatatcgatt cctagttttt
    21841 tcaatgtttg ttcatcattt ttattgacca tcacagtaga gtgggcttgt gctccctcaa
    21901 gttcagctag tttatcataa gcaacttgag cgattggatt cgttactgca ctgattgata
    21961 aagcgatcaa gacttcattg atgcttagtg catccaaaga ggaatgaagt gtctttgatt
    22021 tcaatccttg tattgtttca agaactaatg gtgacaataa aggaatgtca tctgcgatat
    22081 gggctaaatg ttttactgca tttaagataa ctgccgctga cgcatccatc aatgaacttg
    22141 tacgcccggt aatgatgact tcatctggta attcgattgc aatcacagca gaaggatctt
    22201 ttgtaggact ttgcgcttga atcttatctg catagtcatg ggcagggact accacgtttc
    22261 ggtcttcagg acgtaaaccg acttcttcca taatcagttt gattcgatta actgcttctt
    22321 cgtcgatcaa tccttttttg aagtcgcatt ctgtttggaa ataacgacgg atgatttctt
    22381 gtttagaggc ttcttggaca acggtatcat ccacgatccc aaatcctaca cgattcacac
    22441 ccatgtcagt aggagattga taaacagatt ctttaccagt aattttttca ataatacgtt
    22501 tgattacagg aaatgtctca acatcccgat tatagttcac agctacttta ttgtaggcat
    22561 caaagtggaa ggaatcgatc atattgacat cttttagatc gactgtggct gcttcataag
    22621 caatgttcag cggatgtttc aacggaacat tccatactgg gaaagtttcg aacttggagt
    22681 aaccagctgc atgtcctttt tggctttcat gatacagttg gttcaagcaa gtagcgagct
    22741 ttccacttcc tgggccagga gctgtcacta caacgattgg tttagttgtt tcaatatact
    22801 cattttttcc aaaaccatct tctgagacga ttttttctac gttgactgga tagtcttcga
    22861 tttctgagtg tttatagacc ttgatgtttc gtctttctag tttattgata aagactttcg
    22921 tggcaggctg tccactatag cgagtgataa cgacactgtt gattaataag ccataaccac
    22981 gtaattcatc gattaaccgc aagatatcca tatcataagt aatcccataa tcaccacgga
    23041 tcttattacg ttcaatatct ccagcataga cgcaaatcag tatttcagct tgatctttca
    23101 atttttgcag caatttgatt ttcgcatctt cttcaaatcc tggaagtaca cgtttcgcat
    23161 gcttgtcgtc aactaacttc ccaccaaatt ctaaatataa tttgtcatag tggttcactc
    23221 gttccaaaat atacgctgac tgctcttcaa tatatttttc tgaatcaaaa ccaatttttt
    23281 tcactaggtt ccctccttaa gtactaaagc attttttcat ttttttgagg attcgtcaac
    23341 tggaagagcg aaattcctca aaaaaacaaa gaaaatggtt taaaccaact tgtctagata
    23401 gttattcatt ggtatgatag tttttgataa ggaaggtgct acatgaaaaa aagacagaaa
    23461 aagaaaaacg catataaaca ctatatccgt tcgattttta ccggttatga aagaatgctt
    23521 gaagatcccg aattggaaca attgacgttt acttatctaa acgaagaaac gcagctaact
    23581 cgtgatgagc atcagcgcat tcatttcaca acaagagatc tgcctagtaa ataaaaaata
    23641 agataaaggt catcggattc gattctgtcc tatcagatgg aaagactagt tgatcgaaaa
    23701 aaaggctgta ccaaattttg gtgcagcctg ttttatggag cattgcccgc tcttttttat
    23761 aacaattttt tacagaaaat ggtactattg aagtattaat aagtgaaaaa cagatgagta
    23821 agaggaggca agtagtatga aagagccaga tacaaatgca aattatgagc cagacgaact
    23881 agaggagacg aacgcggatc aggatacagg acaagaatcg tcttcttttt acgatatcaa
    23941 agaaaaagca agcatgaatc tttcccaatt attagaagac ttacaggctt ttgaaaaagc
    24001 tgtccgcgaa gaagatatcc ctgagatcta ccgtttatac aatggccgat tgaatgaaga
    24061 attaaaaaga agctcaaatg aaaatcacga gatcgatcaa ttgctgatgc gtaagatcca
    24121 tgatcgtttt ttacaggaat ttccttttat ggaacaagta gaaaacatct cgccaacgat
    24181 gagctattac aaaatcggca cttattacca tgatcggccc acggtcggta ttgatgcaag
    24241 tctgccagaa atttttgttt tgcctaagat cgatgaagag tgggagaaat attcccaacc
    24301 ggagtcaacg gactatgaca aaaaaatcaa tgagctagac gcaaaggtga tcactgctca
    24361 gacagaaatt gaacgtttag atgaacaaat aaaagaaatc aatcgacaaa tgactgattt
    24421 gaacgacaat aaaggattct tgaatcgtaa aaaaatcgag gaagaaattc aagaattaga
    24481 gaagaagaag caagtactat ctaatgaaaa attaggctgg cttccttata ttgagacgcc
    24541 tgaaacaatc cagcagcaaa aagaagcatt gaagcaagaa gcaagagcag accagcttcg
    24601 tgcagccatc gtagaaaaag aacaaaggca aatcagacgc tatttcggta gcaaagaggg
    24661 attcggtcaa gcaatccatg aattcttgat gaattatctt ggcaatgaga atcctaataa
    24721 tcaatctaat gaaggaggtt ccgagtatga atgaacacga tgaagcagaa tggacaaaaa
    24781 atgaagaaca aacagaagat catgaagaaa cgaacacttc attggaagaa caaacaagta
    24841 atcctctgga tgacacagaa gaaatcccta tgctagagga aacaaaaata catgaaaaag
    24901 aaaaagtaac tccgacgaat acgaatactg cagaaaacac ccatgcttac gaaataactg
    24961 aaaatcccgg gtatttagaa ggaaaagagc aagatcatca agatatcgtc agtttgattg
    25021 aaacagcgga agcagaatta gcggatttac gtatgagaaa agatttgatc gaatcagatt
    25081 ttccacaatt attagaatca tatgatcgtt ttatattgaa tgaagaacca atctccacga
    25141 tcggaaagac aagcctgatc gaatacttca cgtatgaatt actgaaaccg ttgaatgata
    25201 ttggtctgga acggtcaaat tcagcttatg acacatggaa aacaagacat ttttcgatca
    25261 attatacgtt gaatggacaa aacgaattag tcttttcctt tgtattaccg accatcgatc
    25321 aacgatacca agtggaatcg atgagtctcc taaacgtatc accagagaca atggaaatca
    25381 atgtccaaga tgaccgggtg ctttctttga tccgacactg gtcagtggat cgagtattct
    25441 ctgcagggca gatcacgata ttcaatcaca aattgaatca attattagcc catgcaagaa
    25501 cacttggttt taccgttaat cagacattac tagacaatac gaagccactt cgtttgaatc
    25561 ttcagagcga atttgaattg acgaatgaag tattggatga tatttttatc gtggcaatga
    25621 aaaatccatc ctacgatatg gaaaagatca acgaaactac ttatcaagta cgattagata
    25681 aaggacagtc tttaacgata acaacaaacg aaaaagatca aacagtcttg tcgatctcat
    25741 ctggagacta cccacgatct gtcattgatt tctttatcaa ttacgaattc ttggtcccat
    25801 taatggtgag aaagtcctga ttggataagt tggattacca gatgtcattc agataattct
    25861 gttccaaagg acttgcggca gttatccaga ataattgtca caagtctttt ctgtatgtac
    25921 taaatggacg tgaataactt gctgatgaag gagggatttc attaggaatg tccacaaata
    25981 aaatagcaca gcttagctta ttgtctgctg cttgtgtagc aggtaggatc gcttttcaat
    26041 ttattccgaa tttccagcca atgactgcta tttttttatt tatagtactt tacttaagct
    26101 taaaagatgc gttaattgtc atgagtcttt ctattgcgat cagcagtttt tattttggtg
    26161 ttggaccatg ggtcatcggc caatggatca gctatactgt tgttctgagt ttgttttcaa
    26221 tcgtctgctt gagaaaaaca gtgcaaagga atctatggtt aaagggagtc tgtttttttc
    26281 tggcgggcat ggtttacgga ataggtatga ccgtttttga tacgctgtta tatcgtttgc
    26341 ctcaaccttg gatctattac cttcaagggg tgtcgtttga tttgatgcac agtataggca
    26401 atgttgtgtt ctttcttgtt tttctgccag tcgtcaaacg tttttacaat cattcaggag
    26461 gaaaaaatga aaaaaataat ttatagtttg cttgcaatga gtgtcagtat actcattttg
    26521 acaagttgca gtacaaccta tacacaatcg gcttccaacc aaaaggagga agtaaaaaca
    26581 tcgcaaaaag caacaatcac gttacaagaa aatggtcaaa acttttcgga aaaagaagtg
    26641 gtcttcaaaa aaggcgatga tttactgagt attttgaaac gaaattttga agtcaaagaa
    26701 gataacggct tcatcacttc gcttgaaggg cactcacagg atgagaaaaa tcaactctat
    26761 tggatgttta ctgtggacgg gaaaagtgct actactggtg ccaaagagat caaattgaat
    26821 gatggacaat cggtcatttt caatttgtct aaactataag tttcataaaa aactgttaaa
    26881 gggcgaatat tgccataatt cagtgagttc agttgatgaa agaaggaaaa taacatggaa
    26941 aattttaaaa aaagcggaat gttttccgta attgctgctc taggggctaa tattctagtt
    27001 gctatcagca aatttgtcgg ctatgcaatc agtggcagtg ccgccatgct aaacgaaagc
    27061 atccacagta tcgttgattg cagcaatcag attttgctgt tattcggtga taaacaagcc
    27121 aataaaggac agagcgagtt gcatcaattt ggtgaaggac gagcaaaata ctttttcagt
    27181 acaatcgtcg caatgatgct tttcttcggc ggtggggcac ttggggtgat ggaagctttt
    27241 gaaaagttga cgcatccatc ccatgaagtg ggtaatccga tcattgttat agcaatactg
    27301 ttatttggtt tggtaatcga aggaagttcg ttacgcgtgg caatgaaaga aatcaaagaa
    27361 ttgaatactg aaaaactttc cacgatgcgc tttttgcgcg aaagtcgaca cagtgaaata
    27421 ctgattattt ttacggaaga cttgtgtgct gtaatcggtt tggtcttggc tttagcagga
    27481 acaatattga cttatgcaac cgggattgct gcatttgatg cgatcagcgg attgctgatc
    27541 ggtttcttgc tgatgggagc cgccattttc ctagcaaaag aattttatag tctgatcatc
    27601 ggtgaaagcg tgacagcaag cgatttatca aaaatcaagt tagcttttga acgtcctgag
    27661 atcagtcggc tgatcgatat aaaaacagtt catttaagtc caacggagat tttagttgca
    27721 gcaaaagcag atatcgttgg taacaaagag aatgaagctt actcggtcat caatgatatt
    27781 gaaaaaaata tgcgtcagtc tcttcctgac aagaagatgt atatatacat tgaaacagat
    27841 aaatatgacc caaattattc tcgtaaataa aacgtttatt catgaattga agcaaaaatg
    27901 agaaaaatat aatcttgttt ttaaataaaa cggctacaat ttagcgaatt tatgttacaa
    27961 tgagtccgct gtttatttgt tgtaattaaa ggagatattt taatgaacaa aaagaaaagt
    28021 gcgccatggc ttcaagcttt gcatgcagtg atgccacttt gtataagcta tataccagta
    28081 ggattagctt gcggagtatt gttgcaaaaa gttggatttg atcctctctt atcaggactg
    28141 ttatcgattc tggtcttttc cggtggcgcg cagtttttag ttgcatctat gcttacgacg
    28201 caagcatcat ttgcaacgac attattgatg gttttctttt tagaattacg ctatacatta
    28261 ttaggagcaa gtcttgccgg ttttatgaaa aaagaaaagc ggacgttttt ggctgtcttt
    28321 tctcaatcac tgaatgatga aaactatgcg gtgaactact taaagttttc aactgaccct
    28381 tcctggaata aaagaaaggc tttatatgtc aattggttct caatgactgc atgggccggt
    28441 agcaatatga tcggtaattt tttcggctcg gtgattcgag tagatgctga ccttgtccat
    28501 tttgctttga cggccatgtt cattttcatg tttatcatgc agatgaaaag cgcactactg
    28561 atctttacag gtttattctc aggcgtatta ggggtcgtat tcatggtatt attccaaaat
    28621 actttcggat tgattgcagc aacagtcatt gcttcatttg ccggatttat gctggagaag
    28681 gcattcaaaa aagaaaaagc taagaaacta agagaccgcg gtaaagatgt caatgagccg
    28741 ctcttcaact tcccggaaca ggaggccaat caccatgact aaactatatt tgctactttt
    28801 agttgcttgt ctgttcatcg tggcatatat cccgcgcttg ttcccgatgc tttattttac
    28861 tcatcgaaaa gtgccatcct ggttcagtga ctggatgaaa tatgtaccgg tggcattatt
    28921 tgcggcgtta gcttttaaag atgttttcat tactcatgaa catctggata ttgcttggaa
    28981 tatcaaaata gctgcaatga ttctagtagc aggcgtggct tacaaaacac gttcgatggc
    29041 gttgtcagtt ttgacaggac ttgcgtctgt ctttttgctg tctatgcttt aaactgtttt
    29101 attttattaa aaacagagat tgcgacataa gttgttcaaa ctgatctatc cgaactgcat
    29161 ttgcaacagt caattctgga tgcaagttta ttcgtaggga taaaaaagat tagtgttggc
    29221 ttatgatgca agctcttttt tattttagga attagagagg gatttgtcct tctatgattt
    29281 tttgctataa tagggaagat gtttatttta tgaaagaata gttgagggaa tttaaatgaa
    29341 taaaaaattg atgcaaggaa cattttggct aacctttgct aatcttctat gtaaagtttt
    29401 aggggtagtc tatttgattc cctggctaag tatgatgggg aacaatcaag atggtatgct
    29461 tgctacgaca ttgtataatg ttggctatct tccttatgga ttgttcctga tgcttggtac
    29521 agtcggattt ccaaatgcga ttgcaaaaaa agtagctgta gccacaaaag aaggagataa
    29581 cgctgcttgt cggctgattt ttagaagtac gatcaatatc atgtttgtca tcggtatcgc
    29641 ctctgctgca ctgatgtatt tatttgctcc tttactagcc ggaatcagtc cgattgctaa
    29701 cgttaataat gggatactag ctatcagaag tttatgccca tctttgattg ctatcccaat
    29761 tcttagtgca atgagaggat atttccaagg aaaaaatgat ctgcggcctt atggaacttc
    29821 tttgattatc gaacaggctg tgcgagtgat cgttatcttg gctggtactt attacttgcg
    29881 cgtattgact gacggaacca tattagaagc tgtattgatc agtacagtcg cttctttctt
    29941 cggtgggtta gctgcgatta tccatatgta tatcgtcgga cgcagaaaag acttcttcga
    30001 acttaaagat tttttgattt ccagccgtta tttcgaaaga gaaaaccgat ctgcctctgt
    30061 ctctatcatt cgggaaacac taccgtttat ttttgtcggc tcagtcatta ctatcgttca
    30121 aatgatcgac caagtaacga tgaaaccact gcttcacttt ttccgtccag agatcgcaga
    30181 tcagcaactg gaattcttat tcagccgtgc ttctgtcaat ccaaataaat tgacattgat
    30241 cctgatctcc atggtaggaa cagtagctat aagcagcttg ccaatattaa gcacgatgaa
    30301 gaaaaaagat cggatacaaa tcgaaaaaac agtaggagat agtttttcga ttgcgttatt
    30361 gattcttctt ccttcattga ctggaatgtc tttattagct ggaccactat acacattgtt
    30421 ttttggctat gatcctgaaa gtgtcggata tttccaaatg gctttgctag cttctttgtt
    30481 tttctcttta ttcacgatcc tatctacgat gcttcaatct ttgaatcacc atcgttttgc
    30541 gatccgatta actgtagaag caattatcct gaaagtcatc ttccaagtaa tcggattagg
    30601 attagttggc ggatacggga tgagcttatc aaatggctta gcattcggta ttgtctttgt
    30661 tcgcggctat cactttatgt gcaaagaata ccgaatagca cctttagcaa agatcagcaa
    30721 ttttttctta aaaactttcc gctccaccct gattatgctt gcagtatgtt ttgccgtttt
    30781 ctttttgctg aatcagcgat tgtcaatgga atcaaaaagc tatgcaatga tttattgtgt
    30841 ggctgtagga agtattggcg gcttagtatt tttattttct caattcggaa aaaatgggtt
    30901 tcgaatgttg aaaaatgtta aacatcatag cagaagcaaa gaataggtta actaatcgtt
    30961 ttttgctgtc acttgaaaaa cggcaactcg tatgattacg cttgtcgttt ttctattttc
    31021 caattttagt ttcatatcta atcttactta tgatatagtt ataaggtctg gaaaaattct
    31081 ttgaaacgag gaaattaaac acatgaaact tgcagtagtt acagatagtt cggcatatct
    31141 gccggaacgt atcaagaacc atcccgactt attcgttatt ccaatccctt tgattatgga
    31201 tgggaaaatt tataacgaag gaatcgatat cgaagcggat gaatattatg gacttttaaa
    31261 aaacagtaaa gattttccta ctacttcaca acctgtcgta ggggaagttt tgggtttata
    31321 tgaggaattg aaagagaaag ggtatgacac gattttaagt atccatcttt cttcagggat
    31381 ttctggcttt gtcaacacat tatttgcgat gaaagatgac atcagcggag taacggtcat
    31441 tccttatgac tctaagatca ctagtatgcc gatgggacac atggtagaag ctgccttaga
    31501 cttaaatgat gaaggtcagt cccttgatga aatacttgca catatcgatc gaatccgaga
    31561 taatacctat gcttatctga ttgtagatga tttgaacaat ttagtacgag gaggacgttt
    31621 gacaaatggt gcagcgctga ttggcagctt attaaaaatc aaaccgatcc tgacattttc
    31681 tgaagggaaa attgttttat tcgaaaaaat ccgttcaagt aaaaaagcat ttgctcgagc
    31741 tgaagaaatt attggtagaa gaaataacga aatacaaccg ccagtcaaac tttacgtgat
    31801 acatgcaaat aatttagaag tagcgattgc ggagaaagag aaattgcaga aaaaatatcc
    31861 aaatgcagaa atcgaaattg gtcatttcgg accagtcatt ggtacacatt taggtgaaaa
    31921 agccatcggc attgccattt cagcgcaata attcctttta ttcaaaaaac ataccaaaaa
    31981 t
//
LOCUS       DAMEET010000026        31236 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.026, whole genome shotgun sequence.
ACCESSION   DAMEET010000026 DAMEET010000000
VERSION     DAMEET010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 31236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.026"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            211..1080
                     /locus_tag="PB780_001044"
     CDS             211..1080
                     /locus_tag="PB780_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HDE0733114.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1289..2314
                     /locus_tag="PB780_001045"
     CDS             1289..2314
                     /locus_tag="PB780_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDE0733115.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2353..3303
                     /gene="manA"
                     /locus_tag="PB780_001046"
     CDS             2353..3303
                     /gene="manA"
                     /locus_tag="PB780_001046"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HDE0733116.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3397..5100
                     /locus_tag="PB780_001047"
     CDS             3397..5100
                     /locus_tag="PB780_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDE0733117.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5102..7135
                     /locus_tag="PB780_001048"
     CDS             5102..7135
                     /locus_tag="PB780_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDE0733118.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7146..7586
                     /locus_tag="PB780_001049"
     CDS             7146..7586
                     /locus_tag="PB780_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDE0733119.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7786..8913)
                     /locus_tag="PB780_001050"
     CDS             complement(7786..8913)
                     /locus_tag="PB780_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDE0733120.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8916..9593)
                     /locus_tag="PB780_001051"
     CDS             complement(8916..9593)
                     /locus_tag="PB780_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDE0733121.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9857..11623
                     /locus_tag="PB780_001052"
     CDS             9857..11623
                     /locus_tag="PB780_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDE0733122.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11638..12075
                     /locus_tag="PB780_001053"
     CDS             11638..12075
                     /locus_tag="PB780_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDE0733123.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12088..13239
                     /locus_tag="PB780_001054"
     CDS             12088..13239
                     /locus_tag="PB780_001054"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDE0733124.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13301..13999)
                     /locus_tag="PB780_001055"
     CDS             complement(13301..13999)
                     /locus_tag="PB780_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HDE0733125.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(13992..15344)
                     /locus_tag="PB780_001056"
     CDS             complement(13992..15344)
                     /locus_tag="PB780_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HDE0733126.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15567..16967)
                     /locus_tag="PB780_001057"
     CDS             complement(15567..16967)
                     /locus_tag="PB780_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDE0733127.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17128..17865
                     /locus_tag="PB780_001058"
     CDS             17128..17865
                     /locus_tag="PB780_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HDE0733128.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            17990..18469
                     /locus_tag="PB780_001059"
     CDS             17990..18469
                     /locus_tag="PB780_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HDE0733129.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18466..19233
                     /locus_tag="PB780_001060"
     CDS             18466..19233
                     /locus_tag="PB780_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733130.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19463..20032
                     /gene="lepB"
                     /locus_tag="PB780_001061"
     CDS             19463..20032
                     /gene="lepB"
                     /locus_tag="PB780_001061"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDE0733131.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20136..20738)
                     /locus_tag="PB780_001062"
     CDS             complement(20136..20738)
                     /locus_tag="PB780_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDE0733132.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20844..21313
                     /locus_tag="PB780_001063"
                     /pseudo
     CDS             20844..21313
                     /locus_tag="PB780_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            21419..22003
                     /locus_tag="PB780_001064"
     CDS             21419..22003
                     /locus_tag="PB780_001064"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDE0733133.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            22006..23079
                     /gene="prfA"
                     /locus_tag="PB780_001065"
     CDS             22006..23079
                     /gene="prfA"
                     /locus_tag="PB780_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDE0733134.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23072..23911
                     /gene="prmC"
                     /locus_tag="PB780_001066"
     CDS             23072..23911
                     /gene="prmC"
                     /locus_tag="PB780_001066"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDE0733135.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23904..24083
                     /locus_tag="PB780_001067"
     CDS             23904..24083
                     /locus_tag="PB780_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733136.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24105..25130
                     /locus_tag="PB780_001068"
     CDS             24105..25130
                     /locus_tag="PB780_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDE0733137.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25305..26549
                     /locus_tag="PB780_001069"
     CDS             25305..26549
                     /locus_tag="PB780_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDE0733138.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26702..27649
                     /locus_tag="PB780_001070"
     CDS             26702..27649
                     /locus_tag="PB780_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDE0733139.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27783..28412
                     /gene="upp"
                     /locus_tag="PB780_001071"
     CDS             27783..28412
                     /gene="upp"
                     /locus_tag="PB780_001071"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDE0733140.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28551..28793)
                     /locus_tag="PB780_001072"
     CDS             complement(28551..28793)
                     /locus_tag="PB780_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733141.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            28914..28986
                     /locus_tag="PB780_001073"
     tRNA            28914..28986
                     /locus_tag="PB780_001073"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28947..28949,aa:Thr,seq:cgt)
     gene            complement(29105..29743)
                     /locus_tag="PB780_001074"
     CDS             complement(29105..29743)
                     /locus_tag="PB780_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDE0733142.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(29866..30498)
                     /locus_tag="PB780_001075"
     CDS             complement(29866..30498)
                     /locus_tag="PB780_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDE0733143.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            30849..31148
                     /locus_tag="PB780_001076"
     CDS             30849..31148
                     /locus_tag="PB780_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDE0733144.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT        10647 a         5457 c         6297 g         8835 t
ORIGIN      
        1 gaggagctat ttttgtttgg ctgttcttat tacttgatgc agaacagctt tttcacaacc
       61 tctttttata agagttttta ctcattaatt tacaaaaaac aagcaaaatg cgaattttct
      121 cacatttttt ttaacaaaat caaagttatt gcttgaaaga agaagaatcc atcgttaaaa
      181 taaagcttga cgtagagggg agcaagaaat atgaaagatt ttcaattggg ggctgccacc
      241 tatttaaaaa gtgtagccat tcgggtgaaa gaccgagata aaatgattga tttttacaag
      301 aacgttgttg gctttgattt gaaaagagaa gaaaacgaat tagccatttt aggaacagaa
      361 gaagctggaa gtgaaatgtt gctgctggaa gaaagtccta gagcgaatga tcacatgggg
      421 gtgatcaaaa agctgaaccg tttttcattg attatcccta gcacagaaga aatggcagat
      481 atcctttgtc gtatccgtaa aatgaattat ccaatcaaag aagctttaga agatcatgga
      541 cgtatgggga ttttgctcgt agacccagaa gagaatgaat tggaaattta ccatgaagag
      601 aataagaacg accaaagaga aattccagaa acaatggatc aagaagcatt attaagaaaa
      661 tctgaagaaa aattcagtaa actatcaaca ggttcttatt ttgaaaaaat ccatttaaat
      721 gttactaact taacaaaaga acgccaattt cttaatcatg tacttggttt gaaggtacat
      781 aatgaagaag agggcatcca tgtattgaat gaaggagatt tccatgttgg tttgacagaa
      841 gctagcggag gcgcaattga tttgcctaca gatgaagtga ttggacttga ttttctgaaa
      901 ttcatgatcg atactgaaag catggaagga ctgattgctc acttgcagca actatcagtt
      961 gatttcttct tagacaaaaa gcacacagta ctcacaatct atgatgctat aggaatcgaa
     1021 tggtggttcg tcagtgagcc aaaagaaaag aataaaactg acattcgatg cgacgcttaa
     1081 ctttttcttt cttaacgatt gtcgcatgtc tcgtcatttt atgaacggtc atactatcgc
     1141 ttattctaga gatagaaaga gaaaatagta taccggggga aactgcgcgg tttttcgcgc
     1201 agtttttttg tgtaaaaaag gttgattcct catagattaa gctttaagct tggactgtaa
     1261 gaatctgata gaaaagcagg agaaaaagat gtatccacac acgaaacagc ttattttgaa
     1321 aaagatggaa gaaggatttt atccaggtgt agcagtcgcc ttcataaaag aaaatcaagt
     1381 agaaacattt acagccggct ttgcccaagt cgttcctgaa aagcagctga tgacagaaga
     1441 tctgttgttt gatgcagcct cactgacgaa agtcgttgca accacttcat tagttttacg
     1501 tctattagaa gatggaaaga tagaactcga tcaaccgctt cataaatatc ttcctgaatt
     1561 tgaaaatcag acaatcactt tgcgtcattt actgacacat acttctgata tcaaaacatg
     1621 gataccaaac agagataagc tatctcagga agaattgatc gccgcttatt tgaagctaga
     1681 accaggtgag caactaggaa aagaagtaaa atatacagat gccggtacga ttcttcttgg
     1741 gttgatgcta gaggaaattt accaagaaac tgcagtggaa atctttcaag aacaagtatt
     1801 ggaaccatta ggaatgatga acagcttatt tcttcctttt ccttccaaaa aaatcgttcc
     1861 cacagaacaa ctgccttcgg gtgaggtttt gcgtggacaa actcatgatc cgaaagcaag
     1921 agttttggct gaacatgcag gtaacgcggg tctctttatt gatttgaaag acagcattac
     1981 ttttgtccaa atgtatttga acaagggaaa aatccgtaat ggtcgttttt tagaagaaga
     2041 gacgattgat tctttactgc aagatcaaac accgaaaaaa aatggctatc gttctttagg
     2101 atgggactta aaaaaggatg tagaagacgg tcatgcgatt ctttttcata ctggttatac
     2161 aggtacgttt ttcttgattg atataaaaac acaagaagcg ttgatatttt tgtcaaatcg
     2221 cgtacatcct gtcgatcatc gtgcggagta tctcatatgt cgagatgaaa ttgttgaaaa
     2281 ctttttgaaa gagaaagcgg tattaccgaa ataatgctat aatagctatg aaaaatgaga
     2341 ggagtttgaa ttatgcagga acctatgttt ttaaaaccag tttttcaaga aaaaatttgg
     2401 ggaggaagtc gtttacgttc agtctttggt tttgatatcc ctaacgataa aatcggagaa
     2461 gattgggcga tcagtgcgca tcctcatggt gtcagtgtag tagaaaatgg cgaattcaaa
     2521 gggaaaagat tagatgaact atggtcagaa cacaaggaat tgtttggcca tccttcagaa
     2581 ccagtgtttc cattattgat caagatctta gacgcagaag atgatctatc tgtacaagta
     2641 catccagatg atgcctatgg gatgaaacat gaaggtgaat taggaaaaac tgaatgctgg
     2701 tatatcatcg atgctgaacc tggtgcagaa atcatctatg gtcatcatgc aaagacaaga
     2761 gaagaactag ctgagatgat caaagatggg cgctgggatg accttttgaa gaaagtacca
     2821 gttaaaaaag gtgatttctt ttatgtacca agcggaacga tccatgctat tggtaaagga
     2881 atcatgattt tagaaacaca gcaaagttct gacacgactt accgcgtgta tgactacgac
     2941 agaaaagatg cgcaaggaca aacaagagag ctgcatatcc aacaatcgat cgatgtaaca
     3001 actgtccctg cgaaaacacc agaattacaa atcaaagaag tccgaaaagg gaattcatcg
     3061 atcgtcactt atttagaaac tgagtttttc aatgtatatg agtgggatat taaaggaatc
     3121 acatctttca aaaaacaagc cccttataca ttgatgacgg taatcgaagg agcaggagac
     3181 cttgtcgttg acggaaaaac atatccatta gaaaaaggaa caagctgtat tattccaaat
     3241 ggcgtttctg agtgggcggt ccaaggtgaa ctagcaatca ttgcgtctga accaggcaaa
     3301 tagttcccag atagctgaaa gcagcagacg gtttttcgtt tgctgctttt tctacataaa
     3361 atccgtccat caataaatag aagtctggtg aaaaagatgg aatacaaatg gaaagcaaac
     3421 aacagcagtc gcgtgtctat gaaacaactg agaaaagctg gacatcactt aaatgaaatg
     3481 atcatgccaa atctaagtct gtttatcgct tggggaatct tgtcattagc aacaaaaggt
     3541 gtgtctggcg atttgcacac cacattagaa gaggtatgcc attggatgat ccagctgctt
     3601 ttacccatct tgatcagtta ttcaggcggg aaatcggtag ggggacaaaa aggggcagtc
     3661 gccggtgcaa ttgcttcttt agggttgatc gctcgttcca atgctccaca aatcgtagga
     3721 gcgatgatta tcgggccgct tgccggcatc gtatatgaac aatttgtttc tagattccat
     3781 gtgaaattca aggctggata tgaaatgctt gtcagcaacc tactagtagg actgactggt
     3841 agcgttattt gtataattgg aatcgttttt attgaaccag ttttaaatag tgttcacttg
     3901 ttgctcgcct ccgttgtgtc ttggctagtc acaatgaatc tgttaccact tgtcagtctg
     3961 atcttagaac ctctaaaagt actatttttc aataatgcaa tcaatcatgg tgtattgaca
     4021 cctttaggaa tcgaattctc acaaacagtc gggtactctt ccttattttt gatggaggca
     4081 aatcctggac ctggtttagg gatcttactt tctatccttt gttttgcaga aaaacaggaa
     4141 aaagtaaatg cgagtggagc attgatgatc catgcgattg gcgggattca cgaaatctat
     4201 tttccatttg tcttgctgcg accgtatcta tttttagctg taatggctgg tggggcaagc
     4261 ggcacgttga tatttcaatg gttagatacg ggattagctg cacctgtatc gcctgggtca
     4321 ctactcgtga tttttgccaa tgcgccttat ccacaactag ctggggtaag tatagggata
     4381 gcaatcagta catttgtgac cttcatttgt gcagcgatac tattgaaatt accaaataaa
     4441 gaaaaagaga acaaaacggt caaagaaagg ggagaaatct tggaaccatc tatcattcat
     4501 tccgtcattt ttgcttgcga ctcaggtatg ggatcaagtg ctatgggagc cgccctgctg
     4561 aaaaagaaat tagcagcaga aaaaatcgat ctttctgtcg gatacagctc tatctatgaa
     4621 ttgaataacc aagtaaatca gctggttatc gtgcaacatg aactagcggg tcttgcaaga
     4681 gaaaaagtac ctcaaagtca aatctatgta ctggatcact ttttggaaac agatatcttg
     4741 ctgtcacgaa taaaagaagc gatccaattt ccaaagcggg aagaaaaaaa agagatagca
     4801 gatatcacag aacaaaaaac gtcgcctgct aatattgtat ttttgtataa aaacaacaga
     4861 cgcggctcac aaactatggg catgacgatt ttaagtcaat tagcaaaaaa agcgaaaaaa
     4921 cagtttgtta tctcaaaaga atcaatagaa aaaatcaaaa tccaagaaga aacgatttat
     4981 atcgcacaaa gagaactagc tgtaactgaa cagctagaag aaaagatccc tcggctagtt
     5041 ctagttgaac attgggtagc tacgaatgaa tacgaaaaac tcgtgaaggg agaaatataa
     5101 gatgtattta tcgcccagag aaaaacaatt attagcagaa tttctcagta gcccgtcacc
     5161 tgtatctatc caaaaaatga tgaatttatt gaaggtcagt aaacggacta tttaccgcga
     5221 attagataat cttgaagtct cattgaaatc ggtaaatgct tctttgaaaa aagcagctag
     5281 aggctcattt gtcattgagg catcagaaga agtattagag caattaaaag aaaatacaga
     5341 tgggaaaagt aatgacttat ccactgctaa acgacaacac gcgattttag ccgagttgtt
     5401 actgacgaca gagccgttga gtcttgctca tttcatggaa tcatatcgca tcagtaatac
     5461 cactttttat gcagatatca aacagttgga agaaagtatt cgccaactgc ctttggaaat
     5521 cattcggaac cagggatacg agatcgaagg accggaaaaa taccgccggc ttctgacagc
     5581 gaatgtatta gaattagaaa tcaatgaata tgaacttttt catagtattt ctttcgatgc
     5641 atcactgaat tatttttttc gattcgttga tccgcaacat ctatctcttg cacgcaaagt
     5701 aatcggggaa gaattgatcc aaaaaaagac caatctaagt gatagaaagc ttgaacattt
     5761 ggttttgatg ctgacattga cgatggagcg tgtgacgaaa aatcatttac tgacaaatga
     5821 gacatataat agtctggcaa acaaagaatt actacagaca gctaaacgat tgttttcacg
     5881 gatcgcttct gagacaaaac aactttatcc agtaaatgag atcgtctttt ttgcaggatt
     5941 attaaatgac ttcacgaact catttgatca gaatttcttt gaagagacgt tcgatacaca
     6001 gcttgcttat ttagtaaaac aactgattca agaagtaagt gaagagacga atgtccattt
     6061 ttatgaagat gaaactttgt acaaaatgct gttgactcat ttatctggtg tgttttcaag
     6121 agccgtttta caagaagaga cactgaccaa tccgatttta gaaaaaatca tggttcaata
     6181 cgaggaagtt gcgaatgcaa tccgtaaagc agtagagcgg atttttcaag aaaaaaaatt
     6241 atccgaagaa gagatcgctt atatggtcct tcattttgcg aattcattgg aacgaagtcc
     6301 gaaagaaagc aatatcagta ttgcaggatt ttcgccaagt ggattagctt ctactagcat
     6361 gttggaacta cgattgaaaa ggtattttcc cttcatccaa caaattcatt tctatcggat
     6421 tgctgattta aaaaaaatcg atttacaaga agaatatgat gttgttattt ctacatctat
     6481 tttaccagga tacggtggaa aatatatttt agtctccccg ctactgctag aagatgaagt
     6541 caagcagcta aaagaagctt tttcgcatat tgagaagaaa aaaaggcaaa aaattacgta
     6601 taacagcaat aacgagtgga tgatagaaga tacttatgag gaaacaatgc atttcatgga
     6661 tcaaatcaat caattactcg gtcgtttttt tatccaagaa ctggataatc ctgagacgat
     6721 ttccgaaacg attcgcagct tatttcctta ttttcctgaa ggactagtaa cggatgaaga
     6781 ggtggttcat cgccggctgc ttaagcgata tgaacaagca cctatcggta ttcctcatac
     6841 aaatatgggg ttattccata catccagctc attgatttct aaaccgatct tttgtatttt
     6901 caatttgaaa catccgttga ccattgaagg gatggataga cagccagtcg atttgtcccg
     6961 tatgttagtt atgctggcgc cttcgccaat ccaagaaaaa gacgggattc ttttaggaaa
     7021 gatcagtgga gcaatcatca tgaatgactt gaacaccgag atcttccgct caggaaataa
     7081 agaaatcgtt tatcagttgt tagcgaaaat cctgattgaa gaaataaaga aatgaaggtg
     7141 aaaagatgat acagctacca gcagaaaaaa tattgctcga tcaatcctat caaaattgga
     7201 aagaagcgtt gaccgagatc ggccagtgga tgacccaaca agggggaatc aaaccggcat
     7261 atatccaatc aatgatcgaa agacaagaaa aagcgagtgt ctatatcggg aatttcgtag
     7321 ccttgccaca tgggactgac aaagatgaag aagtagtaga agaaggaatc tatttagtcc
     7381 aagtgcctga tggtgtggac tttggaacaa aagaggaacc aaaaatcgcg acactcttat
     7441 ttgtagtggc tttaaaaaaa gaacatcagt tagcggtgct ccaagattta gcctttttct
     7501 gttcagacat cgaccaagta atggcgctca gtgatgcgga aaccattgaa gaagtccaaa
     7561 tgatttttga acatggacat atataaccaa tgttcacagt aaaaaaagct gccttgcatc
     7621 aagtaataac ttcaaccaag ataaaaataa gaggagctat ttttgttggg aggttcttat
     7681 tactgcggat gcaagctttt gaaaactatt tattctagaa aaccgtttat ttgaaatagg
     7741 ggacggtgac aaggtttttg tcaccgcccc tattattttt gtgttttaat agctcttttt
     7801 tatatagata gtgcccatac ctatgaataa taaaacgata ctccatatac caagtgtcaa
     7861 gataccattc atgaaagtaa cctgttctga agctagtggc gagaaaatat ctcctcctat
     7921 gatcacattt tgaaaatcaa cataagaagt tggcaagtaa tgactgattg attctatcca
     7981 tccaatcgaa gaaaacaagc gatactctgc taagaaaaga caaagaagtg tacctaaaag
     8041 attgactaga gaatgttcaa ataaagcaga tagaagaaaa ctgattgtac ttaaaaagag
     8101 cacccacaac aacaggaaca gaaagctctg gaaaagatac tcatacatcg gcaggattcg
     8161 tatctcctga ccaatgatcg ttcctacagg ataacggaag tctcccaaac catttttgat
     8221 tattactagt atagagacga tcaaaaatgg catcacgaga cttagcatag ctgcaccata
     8281 ataaatggta aaacggatgc tatgtttctt taccatacta tccggtaata gattgactag
     8341 agagattacc tgctttcttt tttcataaga gatgatggaa gaaagaaaca aggcggttat
     8401 acataaaatc aaaaaactag aaatcattcc tgaaaaaata ttttcgtagt aatttgctaa
     8461 agagagatgc gctggcaagt caaaaataga atatctttgg atctgatgtt ctttcatata
     8521 acggagcaac tcgacacgtt tttgttgttc aatgattgga attccttgtc tttgaccaga
     8581 aatcagtcga tccagtatat ctttttcata atgataagtt gcttcgacca tttgctggac
     8641 attccctgtg ttttttgcat gtaagattgc ttcgatcagt ttgttgcttt tctctaaact
     8701 ttccacaaat tctttctcat tctcatcttc tttcaacgtt tcaatcaaac gttgcatctc
     8761 ttcatagtta tgattcagtt catcgaattc tgtataagca agtttatgat caaaaaaaag
     8821 tgggaatgca aagagcacga ccaacaatag aggaaataaa aagtttactt tgttttttag
     8881 taagagattg cgttcaaatg taagtaaaga catagttaca cctcatataa tttacgataa
     8941 atagctaaaa tctcttccat atttgaggat tctgtgacga tttctccatt ttttagaaaa
     9001 catgctttag aacaaatcga ttggatattc gcgatagcgt gggaagaaag aagaatcgtc
     9061 ttcccctctt tctgccattg tctgatgagg ctattcaaga tatcaatact tcctgggtct
     9121 aatccgttca acggttcatc gaataacatg acagatgaat cactgatcaa gtaagcagca
     9181 agcaagacat gttgtttcat tcctagagac atttttccaa tcggcaaagt aaggtactca
     9241 gtcatcttca atcgttggaa aacagtttgg cattctactt tgctctgcca atagtatttg
     9301 atcaaagtca aataatcaac agccgataga tagtcggata actgattcgt tgattctaga
     9361 aaaaagaaag accggcgata atctgtataa gcagtaggag agatgttgtg aagggacaat
     9421 tgatcataag ttgttggcag cagttgggca atgccattca gcaaagttgt tttgccaaat
     9481 ccgttcgggg caatcaaagc agctacttct cctgattcta ctaaaagatt caattgtcga
     9541 atcacacgtt ttttaccacg tttcatgttc atgtttttga tcagtaatgt catagatagg
     9601 cctccaatat aaaatttaat aacataatag aatggaaaac atctaaatta aatgctaaca
     9661 aaagttattg tttgcatatt ataaaaaaat taaaataaca gttttattta aaattgaagt
     9721 tttctgatac cgaaatcttt ttattttgct gacatactca aacctttggc acaaagtttc
     9781 gtgccaaagt acgactattt ctctttttag taagcggata caattagagt atcaaaagga
     9841 gagagggaga gacaaaatgg aacggacgat tcccaaaaac aacatttcgc taaaagcgag
     9901 ggtacaaaaa ttaggcagta ctttatcaag catggtcatg ccaaatattg gagcactgat
     9961 tgcctggggt gtattaacgg ctctatttat cccagatggt tatttaccaa atgaggcttt
    10021 cgctaccatg gtaagcccaa tgctaactta tctgattcct ttattgatcg gttatactgg
    10081 cggaaaagta attgctggtg accgcggatc agttgttggg gcaattgcaa cgatgggtgt
    10141 gattgtcgga acagatattc caatgatgct aggcgcgatg atcatgggac ctcttggtgg
    10201 atatgccatc aagaaatttg atcagctatt ccaaaaacgg atcaaatccg gttttgaaat
    10261 gctagtcaat aatttttcag caggtcttat cggttttgga ttagcgttat taggtttttc
    10321 tgcgattggt ccagttgtcg atgcattgac tcaagcaatg gcaaaaggtg tagagatcat
    10381 tttatctgcc catttgattc cattgacaag tatttttatt gaaccagcaa aaatcttatt
    10441 tttgaataat gcaatcaatc atgggatttt gacaccattg ggaactgaac aagtagcaga
    10501 ggctggaaaa tctattctat tcttgcttga agcaaatcct ggtccaggac ttggcgtatt
    10561 acttgctttt actttgtttg gcaaaggagc agcaaaatct tcagcaccag gagccatgat
    10621 cattcacttt ttaggcggga tccatgaaat ctattttcct tacgttatga tgaaaccgtt
    10681 attattctta tcagttatag gcggaggaat gtcaggaagt ttcattttcc aattgatgga
    10741 tgctggacta agagcgccag cttctccagg gtcgatcatt gcgattctag cgatgacacc
    10801 gttcaattct taccttcctg ttattttggg cgtgattgtt gccgcagctg tttcgtttgg
    10861 tatttctgca gtaattttga aagcggatgc gaaagataca aatgaagact tcgaaaaatc
    10921 tgttcaagag acaaaggcag ctaagcaagc ttccaaaggt gcccaaggta ttcctcaagg
    10981 agctacattc tctggcgtac aaaaaatcat ttttgcttgt gatgcaggaa tgggatcaag
    11041 tgcgatgggc gcttcgatct tgagaaaaaa agtacaggaa gcagacttgc ctcaaacggt
    11101 gaccaatcaa gcaatcagcc aactgacaga tgactcgaat acattgatcg ttactcaggt
    11161 cgaactacaa gaaagagcaa aacaaaaagc accgagcgcg cgttttgtct cagtagaaaa
    11221 ctttttgaat tcacctcgat atgatgaaat cattcaagaa ttaagcagca agcagaaaac
    11281 atcagtttct gaaattgaga atgagccagc agaagagttt ctttcagcaa acatcaaaca
    11341 aatcaaagag attctacttg tccacgatga tcgttccgga tcagcaacaa tggggatgac
    11401 cgtgctcaaa gatatattag acaagaacca tttaactatc ccgttaagaa aagtcaatat
    11461 ccaagattta aaagatgaac ctgattcact gatcgtagcc aaagaaagtt tagcggaaaa
    11521 agtacagatg gctgcacctc aatccattca tttatcagtc aacagcatgg tcaatccgca
    11581 aaaatacgaa tcgattgcac aatatttgaa acagacagct taagggagga aaacagcatg
    11641 caaagtttgc cattcgacat gatcgagctc aatcgatctt ttaaaaccaa agaagaagcg
    11701 atccgctatt gcggtcagat gcttgtagaa gcagggtgcg ttgatgagga atatatcgat
    11761 gccatggtag atagagacaa aatgttatct gtttatatgg ggaattttat cgcgattcct
    11821 catggaacag atgaagcaaa aaaatatgtg aaaaaatccg gcatctgtgt cgttcaagta
    11881 ccagatggtg tagattttgg taccgaagaa gaagaaaaga ttgcgactgt tttgttcggt
    11941 attgcaggag tcggagatga gcatttgcaa cttgtacagc aaatcgcact ttattgcagt
    12001 gatatggaca atgttgtcca gctagcagac gcgctcagca aagaagaagt aacggaaaat
    12061 ctagcaattg cttaaggaga gagaaaaatg agagcagtac attttggtgc aggaaatatc
    12121 ggtcgaggat ttattggaga ggttttggca gctaatggat ttgagattac tttcgtagat
    12181 gtgaatcaca cgatcattga tgctttaaat gaaagaaaag aatacgaaat cgaattagca
    12241 gatgaatcaa aagaaaaaat cactgtaaaa aatgtcaaag gaatcaacaa tcaaacaaat
    12301 ccagaaaaag taatccaaga aatcgctgaa gcagatttag ttactaccgc cattggcccg
    12361 aatatcttac cttttattgc tgagttgatt gcaaaaggta tccaagaaag agaaaaggaa
    12421 ggaagcacaa catctttaga tatcattgcc tgtgaaaata tgattggcgg ttcagcattt
    12481 ctagaaaaag aagtgtataa atatcttccg gaaacatcat ttactgacaa atatatcggc
    12541 tttccaaatg cagcagtcga tcgaatcgtc ccattacaac accatgaaga tcctttattc
    12601 gtccaagtgg aacctttcaa agaatgggtc attgacgata gccaaagaaa aaataaaaaa
    12661 atccaattaa aaggggtttt atatgtagat gatctagaac catatatcga acgcaaacta
    12721 tttagtgtca atactggtca tgcgactgtc gcttatacag gtgccttgtt aggttaccag
    12781 accattgatg aagcaatgca agatgcgtta gttgtggcac aattaaaatc agttttgcag
    12841 gaaacaggaa gtttgctgat tgccaaatgg gggtttgatg cagagcagca tcatgcgtat
    12901 attgaaaaaa tcattcatcg cttccaaaac aaaaatatct ctgatgcgat cacacgtgtc
    12961 gctcggacac cattacgtaa gttaggttat caagaaagat ttacacgtcc agtgagagaa
    13021 ctacaagaac ataacttgac ttgcccacat ttgacggcaa caatggggat tatttttaat
    13081 tactatgatc ctgaagatga acaaagccgt caattgcatg aaatgaagat ccatgaaaac
    13141 ttggaacaat tgattcaaga agtcacagga atcaatgatc cgaaaacaat cgggaatatc
    13201 aagcaaaacg tgaaccgtta tgccaagcaa gtcgcttaaa ataggatacg aaaaagggga
    13261 atgtgacgaa atttttgtca cattcccctt tttcgaataa ctaggctgtt tgtccttcaa
    13321 gatagggcag atccaattct ttaccgtaac gttgttctaa agctaggcgg atcaaacgag
    13381 ctgccaggtt ctcatttctt gcaaggatag gtccatggaa ataagaaccg aagacatttc
    13441 ggtaaatgac accttcagat tggtcttcac cattatttcc ttgaccttgt accactttcc
    13501 ccaaaggtcg ttcaccttca cctaagaaag tccgaccatt gtggttttca aatccataat
    13561 aagtttcatt gaattcttcg ttatggataa cgatatcacc gataaaccgg ttattttcct
    13621 gacttaacgt gtagtgatcc agtgcactga ttccttggat cttttctcca ttagctccca
    13681 tgtagtagtg acctagcaat tggtagccac cacaaatggc taacatgacg ccatcatttt
    13741 cgatatagtt gatcaaagaa tctttctttt tctggatgtc ttttgaaatg attaattgct
    13801 caaagtcttg accaccgcca aagaaaacca agtcatattt atcagcatca aaaggttcat
    13861 gtatactaac gatctccgta tggcaagaaa cccccatttt ttctgctata tatttcaaca
    13921 tcaaaaggtt tccgttatca ccatatgtgt tcatcaaatt tccataaagg tgggcgatat
    13981 tgagttcata attagccacg gttcattcct cctttgataa atccttgagc agctagttct
    14041 ttacgaagct gcaatacagc agtgtaagta gctaaaatat agacatgctc agttggcaat
    14101 tctttgattt tttcgattac ttctggtaat tcttttgttt ctgtcaattt attttctgga
    14161 atcccggcaa cttttagacg taaagtcata tctgtatgac ggtctcctcc agaaataaca
    14221 gctgggatat ccatatcggc aaagttttca aaattaccat cccaaatcca gctgacatcg
    14281 attccgtctg cgtaattggc atttaataaa gccactaaag aaaatggata aggagctaga
    14341 ccaatcatat cgattacttg attgatacct acaggatttt tgacaagtac taatgtacat
    14401 tttttcccat tgatattgat gacttcttgt cggccgaata ctttctcgtc atatcctaaa
    14461 ccttgtttga ttttatcagg atcgacattg aagtactctg ccactgcagt agctgctaaa
    14521 gcattgtaga cattatacat accgccaact tcgattccgt attcatgacc atcgatcacg
    14581 aaatctgctg acacattgtc catccgtacc atatcagtta atcgataatc caattcagga
    14641 cgtttgaagt cacaatttgg acagtaatat ttccctaagt ttgcataagt gatcatttta
    14701 taatgcaaga tgtgatgaca atttggacat aagacaccgt ctgtattgta atgagccatt
    14761 tgttcacgat cttcttcatg gtcaaagcca taatattttc taggattgac tgtttcgata
    14821 gaattgaaga ttggtgaatc accgttactg ataatcgttg catttggtga ctttgcggca
    14881 ccgtctacaa tcattttata ggtcgtatag atttctccat accgatccat ttgatcacgg
    14941 aaaatatttg taaataagaa cagttctggt ttcatatatt ctgtgacttt gctcaaactt
    15001 gcttcatcta tttctaggac agcaaacttt ttctgtcctt tttttggttt tgcctgcaag
    15061 aaagtcgata caattccctg taccatattc gcaccagtag ggttggttag aacttcatcg
    15121 aattgctggc gcaaaatatt gactgttaaa gcggtagtca atgttttacc gtttgttccg
    15181 gtgacaacga cgacttggta gtcttttgcc aaactatcta gtatatgtgg atcgactttc
    15241 agcgctagtt tacctggtaa acttgatccg cctttaaaaa aagtttttaa tgcccattgc
    15301 gacgtttttc caactgcaat cgcaaaatga ctgcgtattc ccattatgta acctccaaaa
    15361 acgatttgct gacgaataaa ttcaactaat cataccatat gttgaaaaaa aggttaaatt
    15421 gcttttctta ttcttaagaa aaggttacgg ctgaaagtgc atgaaatcgg acaaaatcgc
    15481 atgatttaac aaaaaaacag cttgaaacaa aatgattgtt tcaagctgct gtgattttat
    15541 tattccctct tacctatatt cccattttaa ccgataataa ttttctcgct tggatattca
    15601 tagcctagat ctcgtgtttc ttttggcacg aataacatca atgtatatag cattccgatc
    15661 cgcccaataa acatcagtgt agcaatcgtg attttgccca caacagacaa gtctccggta
    15721 atccctaaag ataaaccagt tgttccaaac gcagaagtta cttcgatgat gatcgagatc
    15781 agcgtttgtt cttctgtagc agataaaaaa acgatacaaa agaaacacat accaagagaa
    15841 agcataaaga caacgactga ttttcgcaca tcatcttgat cgattctccg tccaaaaata
    15901 ttgatgttat cttcactttt gaggaaggaa tatagataca aaccgatgat tgctacagta
    15961 gttgttcgaa tcccgccgcc tacagaacta ggactgcatc cgataaacat cagtaaagaa
    16021 aaaatgatca gtgttgtgat ttgaaaatca cccaaatcgt gaatctgcag accagcatta
    16081 cgagtcgtaa tcgaataaaa catcgaattg atccatttga cactcatatt ggaatcttgg
    16141 aataaatggt ccttttccaa tagataaatc aagacagttc ccgaaacaaa caagataaca
    16201 aaagctaaca cagctaattt ggtaaacaag gaaaaacgaa acggaagctt cgcattggaa
    16261 cgtttgtata atagccattc ccggcactcc atcaatacag gaaaaccaat tcctccgaca
    16321 aaaatcaaaa acatgatcag gatcaagaaa aaatagtcat gtgcaaaggg cttgatggaa
    16381 tccccagtga cgtcaaaccc agagttcgtg acagccgata tcgcctgata aaaaccgtaa
    16441 aaaacggcat cacgccaacg atcaaaatag cctctgtaat aaaaataaat agaaaagaat
    16501 gtcccaaaaa gtaattgaaa ccagattaaa atagcaaaag tgatccgaat cagtcggacg
    16561 ataccagaca aacgcggttg attcatatcc gtcatgatca gctgtcgctg tttcagtgtg
    16621 atccgtcgtt tagaaaaaat gacaaaagca gtagaaatca tcatgatccc caaaccacca
    16681 acttgaaata atatttccag cagtataata ccgttatcat tgaacacaga attgatatca
    16741 aaagtcgata gtccagtcac actcactgta ctgattgcca tgaacagcat gtccacaaaa
    16801 gaaacatggc tatctggttc gcgaaaaatc ggcatataaa atagaacgag tgataagacc
    16861 gtcatcaata tataataaaa aacgatgatt tggatcgatg agaaatgatt ggcagcaaac
    16921 ttctgtcctc gccgctgcag ccttcgaaag aataaatcaa catgcacagt actatcctcc
    16981 tgacaatagt cgtttctacc agtatacaag gggaattagg aatactcaaa gaaattatca
    17041 aacagataac aatacagctt tcgattaaaa tagaaatggc ttataataag gaaaatccta
    17101 gaaaaaagaa ggaggaggaa taacaaattg attaatgctc aacaaatcgt agaaaaaatg
    17161 aaaaatcaaa aaataaatta tgatacagtt ctgaaaaaaa tgatcatgca atgggagaaa
    17221 gaagaagtaa agcctaagat attaatacat acatgctgtg ctccttgcag tacgcacaca
    17281 ttggaattca tgtctcaata tgcagagatc acgttatttt tttcaaattc gaatattcat
    17341 cctaaaagtg agtatttgcg aagattacat gaacaaaaac gatttgtaga ccaatttaat
    17401 gagaaaactg accatcatgt tgcgctaatc gttgatgaat accgtccaaa tgaattcacc
    17461 aaaatggttt taacaaacca tcttgaaaac gaaaaagaag gtggtgctcg ctgcagtgca
    17521 tgcttcaata tgcgattaga tcttgttgcg caaaaagcgc aagaactagg atacgattac
    17581 tttggaagtg ccctgaccat ctcgccaaag aaaaatgcag ctttgatcaa tcagatcgga
    17641 atggatatcc aaaagattta cgggacacaa tacttgccaa gtgatttcaa gaaaaacaaa
    17701 ggctatgaac gctctttgca aatgtgcaaa gagtatgata tttatcgcca atgttattgt
    17761 ggttgcgtgt tcgctgccaa gcagcaagga tgcgatctga aagaaatcaa tcgggaagca
    17821 aaagcttact taaaaacatc aaccgtagtt tttgaggaaa attaaagaaa tctttcggaa
    17881 aaacgtaaga aaaaaataaa gaatttatgg tagaaataag gtagactgta taatttgtcc
    17941 gtagtaaagt aaggaaacaa gaataaattt caatgaagga agagataaga tgcgaaaagt
    18001 agcacctatt ttgaagccaa tcgctgtatt aacaatctcg acgattgtcg cgactactac
    18061 aggtattgtg aatagttcca gctaccgtgc accaagttca agttctagta ctgtagaaga
    18121 aacaacacca actacttcag tctcgacatc tgaaccaaca acaacggaaa gtttgaatga
    18181 aaccactacg acaagtacat gggacgatac aacaactagt tcgtcgtatg atgagacata
    18241 tacaactgac agctcaagcg aaacaagtaa tacggtacca gatggcactc catcggattc
    18301 aaccaatcca agtacaagtc cggatacaag tgctagctcc gaaacgatac caagcagtac
    18361 gactgtagat gataacacgc aaaatgatga tgcagcacgt acagcacagc aaaaccaaga
    18421 ttcaggaaat acccaaacgt ataaccagca agcaggtgat tctcaatgac atttttacta
    18481 gaccttatca acggggtgca taaattcctt ggctatttag acatcagtcc taaatatctt
    18541 aaccgtggat atacgatttt aagtgtgatt cccacgcttt atttattgag aattgtttat
    18601 ggactttggc aaaaccaaaa ctatcttcaa tttttcttat atggacttgc atttctagtg
    18661 cttgtttatt ttactgtttt aaatgtattt tattactttt tagataaaaa cacgaaagcc
    18721 gatgtcactc agctttttgt gaagtattta cctgatgaag cgttcaacat ccagacagaa
    18781 gaatcgatag ataatggaaa ttcgatcgat aaagtgaata cacaagaggt catggtttct
    18841 tatgacgaag attatcaatt gaaattagcc gagaatatgc ggtatttgat tggaaatggt
    18901 gagatcaaaa caaatgaact cggtcgcatg gatggatttc tggtcgatag aaatacattg
    18961 tatccttatt actatgtcaa gaaaacagga gagaaagagt atgttctaaa tattggacga
    19021 agctactcgg acatggaaaa agtagggacc attcgacttg actcaacaga agacacattg
    19081 gaacctgtcg gattgttcat cgtcggcggt gattttgtca aagacgggtt acgctatcat
    19141 gagccttatc gtctgaaatt gattgcaaaa aaggcacagt cgcaggaaga accagtggtt
    19201 tatagtcgta gccaaagaag aagacattca taatgtgatg aaacgccgtg tatcagtggt
    19261 tgcgataaaa acgtttattc ggaataaggg ggatgtgaca cgtcttttgt cacgttcctt
    19321 ttttatataa cgagaaagat aagacgaaca catcacttat tctttccttt catttataaa
    19381 cattgtagaa taaagaaagt acaacgaagt attgtgaaaa aacgtaataa ataacgagga
    19441 aaatacaagt aggaggtaag ctgtgaaaga atttttgaaa aattggggga ttttgatcct
    19501 tgtattagcg gctattttat tagctagagt gtacgtgttt acaccagtta ctgtaaacgg
    19561 gcattcgatg gaccctacat taagtgatgg acaacgattg atttcatcta agatctcaaa
    19621 ctatgaacga atggatatta ttactacaaa ggaaccgggg gatgaagaac ggatgattgt
    19681 taaacggatc atcggtatgc ctggagatac agtcaagatg gaaaatgacc agttgaccat
    19741 taatggcaaa aagtatgatg aaccttatct ggatgagttc aaaaaagaat tttcagaaga
    19801 taagttacaa ggagaatatg cttatagctc aggattccaa gcacaagcag aaagttcatc
    19861 cacctttacg agtgactttg aatatactgt gccaaaagga aagtatcttg ttctaggcga
    19921 taaccgcttg atttcaaaag acagccgaat gtttgggtta gtcgacaaag atatgatcca
    19981 aggaaaagtt gttttccgtt attggcctct ttcagaaatt aaaatcatgt gatgaaaaaa
    20041 taaaaagaag ctgtgacatg ttttttgtca cagcttcttt ttccgaaaat tccttcttat
    20101 ttcttggagt tgctcacttt ttttgcaatc tcattttaag ctttttggct gatggataag
    20161 aaaagcgaag cgtttttgtt cataaatagc ttttccccac gatggaaggt atgactagta
    20221 gcagagacaa agcccgtttt ttccaatcga ttattcaatt cttcttggct aaagccatta
    20281 tggactttcg gatgactaat ttgttcattc ttgtcaaagt ctacgatgat caattgaccg
    20341 ccaggagcta aaatttcata aagttttgta agaatattct ccgtatcagg aatgtgtaaa
    20401 agtacaagtg atagaaggat gagattagct tggacagcag gaaattcgac agaagcatca
    20461 gcgtggatcg ttcggacatt tttcagatct gctgcttgga tcttttcttc tgccattttc
    20521 agcatagttt ctgaagcatc ggcaatgatc agttctttga atctttccgc aagcggtaaa
    20581 ctaacaagcc cagttccgcc tccgtaatcc agcaggactt tttggtggct gtcgcgcggt
    20641 agataagtag ttagttcttc gcgaataatc ttggctaatg cttgacggtc aggggaatca
    20701 taatggttag cgatggaatc aaaaatattt tttgtcatca aatagctcct tacgtaaaac
    20761 tttgaaattt taacttatta tagcacgtga aaagaaagaa agcatgctat actcaattca
    20821 aaaagggaga atctagaaag aatatgccga tatcgtttgt aaaagataga gaggaaaaag
    20881 gaaagtgtgt ccgagaaata ttactagact tgcctgagtg gtttgggctt cctgaatcaa
    20941 cggaaaaata tatcgaggaa tcaagtaaac tacctttatg gtgtgaaaaa agaaaagaag
    21001 aatatttagg ctttatcact ctttcacaga caagtgaaga tactgccgaa atctattcta
    21061 ttgtatggga ataaaaaaag actatcatca ccaaggatta ggaacaaaac tttttaaaga
    21121 agcagaaggt tacgcagcaa aacattacaa atatttgcaa gtgaagactg tagacgaggg
    21181 gcactactct atatatgatc aaactatttg tttctacgaa tcattaggat tttcaaggtt
    21241 ggaagttttt ccaaatttat gggatgaatg gaacccatgt ttagttcttg tcaaaaaatt
    21301 ggaacaaaag tgagcaatag atttaatact aatctgtgct tgatgaaaat tctcactgta
    21361 aaaaatgctg aaaatcaagt ataataccat aggaacaaac tggaaggtga aaaagagcat
    21421 ggcacaacta ttttttaagt atggcgcaat gaatagcggc aagacgatcg agatcctaaa
    21481 agtcgcacat aattatgaag aacaaaataa gccagttgtc ttaatgacaa gcggtattga
    21541 tacaagagat ggtttaggcg ttgtttcaag ccgaatcgga ctgaaacgta atgcgatgcc
    21601 gatatttgaa gaaacagacg tatttgacgt aattcagaaa atggaaagca aaccctattg
    21661 tgtgctgatc gatgaagcgc aattcttaaa aaagaaacat gtcattgctt ttacgagaat
    21721 tgtggacgaa ttggatattc cggttatggc atttggtttg aaaaatgatt ttcgaaacga
    21781 gttatttgaa ggatcaaaat atttattgct ttatgcagac aaaatcgaag aaatgaaaac
    21841 aatctgttgg ttctgccaca aaaaagcaat catgaattta cattatattg atggaaaccc
    21901 tgtttatgaa ggcgatcaag tgcaaattgg cggaaatgaa gcttattacc ccgtttgtcg
    21961 aaaacattac tttcacccat caatgattac agaaggagaa taagcatgta cgatcaatta
    22021 caagcaattg aagaccgtta tgaagagctt ggagaacttt taagtgaccc agaagtgatt
    22081 tctgatacaa agcgttttat gcagctttca aaagaagaag caaacacgcg cgaaacagtc
    22141 gaagtttatc gtcgttataa agaagtagta caaggaatca ccgatacaga agaactgctt
    22201 ggtgaaaaac tggatgatga aatggctgaa ctagcaaaag aagaattatc tgaattaaaa
    22261 aaagaaaaag aagtcttaga agaaaaaatc aagatcttgc ttttaccaaa agatccaaat
    22321 gacgataaaa atatcatcat ggaaatccgt ggagcagcag gcggagacga agcagcatta
    22381 ttcgctggcg atcttttcaa tatgtaccaa aagtatgctg aagcacaagg ctggaaaaca
    22441 gaagtgatgg aagctaacgt taccggaatc ggtggatata aagaagtgat catgatgatc
    22501 accggagaca acgtatattc gaaattaaaa tatgaaagtg gtgcgcaccg cgtccagcgt
    22561 gttccttcaa ctgaatcgca agggcgtatc catacttcca ccgcaacagt tgtcgtgatg
    22621 ccagaagctg aagaagtaga gatcgatatt gaagacaaag atatccgtac agatatctat
    22681 catgcttcag gtgctggcgg acagcacgtc aataaaacag catctgctgt acgtttgact
    22741 cacttaccaa cagggatcgt tgttgctatg caagatgaac gttctcaaat caagaaccgt
    22801 gaaaaagcaa tgaaaatctt gcgtgctcgt gtgtacgata agatccagca agaagcacaa
    22861 agcgagtatg atgccaatcg aaaatcagca gtcggaactg gggatcgttc agaacggatc
    22921 cggacgtata atttcccgca aaatcgtgtg actgatcatc gtatcggatt aactatccaa
    22981 aaattagatc agattttagc agggaaatta gatgagatcg ttgatgcatt gatcatgtat
    23041 gatcaaacat caaaactgga agctatgcaa aatgggtaaa atgacctatc gagaagtcct
    23101 gtcacgggct tcttcttttt tagaggatca tgggaaagaa gcatatagta tccaattttt
    23161 atttttggaa cgaaaacaat ggaaaaaact tgattggctc cttcatatga acgaagagat
    23221 ttcagaagaa gaacagcggt tgatcgaaac cgatctgcga cttctgcttg cagatcatcc
    23281 acctcagtat ttgctaggat atgcagattt ttatgatcat cggttaaagg taactgaagc
    23341 aacactgatc cctcgaccag aaacagagga gttagtcgaa tggtgtttag acgagacgcc
    23401 tggtgtaccg ttagaagtga tagatattgg gacagggact ggagcaattg ctattagttt
    23461 gaaagcagca agaaaaaatt ggcatatatc tgctgtggat ctttcagaag aagcattaga
    23521 agtagcgaaa gaaaatgctc agaaagaagg aacgaaaatc agtttttatc atggagatac
    23581 attaggacca gtaatggatc agcaatttga tgtgattatt tctaatccgc cgtacatcag
    23641 tcggaatgaa tgggaactga tggatgaaag tgtccggtca ttcgaaccta aaatggccct
    23701 ttttgctgag aacgatggat tagctattta tgaaaaaata gcagttgaag caccaagcgt
    23761 actaaaatca aatggaaaga tttttttaga aatcggattt cgccaaggtg aagcagtaaa
    23821 aaaaatattt cagcaagcat ttccagataa aaaagtggcg ataaaaaagg atctttttgg
    23881 caatgaacga atgatccaag tttatggttg aagttgtgga tgaaaaattt tttatttgtt
    23941 tttttattat aaaaaaacac ttatttgaca aggatgtaaa cgactttttg gtttatcaac
    24001 aagtttatcc actattgaca aatagattat ccacaaacag tggaaaactt gaggataaac
    24061 tcagacaaaa taagaatgta tgaaaaaggg gttgtggata aactgtggaa acgaaacgat
    24121 atgatgaaac agaattgcaa gaggcagccg aagcactgaa agaaggagaa cttgttgctt
    24181 ttcctaccga aacagtatac ggattgggcg ccaacgctct tttaccaaac gctgtcaaga
    24241 aagtgttttc tgtaaaaggg agaccgcaag ataatccatt aatcgtccat gtcgcctctt
    24301 ttgaacaagt aaaagagtat gtggacaact ttcatccggc agcagagaag attgtgaaga
    24361 acttctggcc tggaccatta actttgatat ttaagatcaa aaaagacact ttgccctcag
    24421 ttgtgacggg aggattatcc acagctgcat ttcgtatacc ggataacaaa aaaacactag
    24481 aagtaatcga actttccggt gttccgcttg tcggtccaag tgcaaatacg tcgggaaaac
    24541 caagtccaac gactgcagat catgtatatc atgatctaca aggaaaaatt accgggatta
    24601 ttgatgatgg agctacgcga attggtgtag aatccactgt tcttgattta agtgatccaa
    24661 cagccatgcc tatgatttta cgaccaggtg cagtaacgaa agaacaaatc gaagcagtca
    24721 tcgaatctcc tgttgctatt gatcagcatc ttgtaaaaga aaatgaaaca ccaaaagccc
    24781 ctgggatgaa atacaaacac tactcacccg atacacgtgt tctgatggtc agagatggcg
    24841 attggtcaac tgcagttcaa tgggcaaaaa ataagaagat ccgtgcaggt gtcatagcaa
    24901 gccccgaaat tgcggatcaa gtgcggacag atactgcagc tgtttatatg tataacgaca
    24961 actcagtaga agcggcggct aaaggattgt ttgcaggtct aagaggatta gatgaaccaa
    25021 cacttggatt agatttgatt tttgtacaag tgtatccaga aactggattg gggaatgctt
    25081 acatgaatcg cttgaaaaaa gcagcaggac aaaattattt tgaaaaatga tcaattggtt
    25141 attgtcgcat tcttttagta tgatacaatg ctatcaagta aatcttctat cagtgaacga
    25201 aaaactaagc gtaactaatc gtaatagaag agatacgaat atttggcaat ttgtttttga
    25261 tgggaaaaat aaaattgtag gaaaataaaa aggagctgtt gtttgtggta gattacaaaa
    25321 cgtttgaccc tgatctttgg gctgcgatcg ccaaagaaga agaaagacaa gaacataatt
    25381 tggagctgat tgcctctgaa aactttgtct ccgaagcagt gatggctgca caaggaagta
    25441 tcttgacaaa taaatacgca gaaggttatc cagggcatcg ctattatggt gggtgtgaat
    25501 ttgtcgatat cgttgaaaat ttagcgattg accgtgcaaa agaattattc ggagcgaaat
    25561 ttgcgaacgt ccagcctcac tccggctctc aagccaacac ggctgcttat ctcgctttgg
    25621 tagaaccagg ggacacgatc ttaggaatgg atctttccgc aggcggacat ctaacacatg
    25681 gatcaccagt caacttcagt ggcaaaacct atcactttgt tgcttacggt gttgatccga
    25741 caacagaagt tattgattat aatgtggtac gtattttagc tagaaaacat caaccaaaat
    25801 tgatcgtagc aggtgccagt gcttacggac gaacgatcga ctttgcgaaa ttcagagaaa
    25861 tcgcagatga agtaggcgca aaactgatgg tagatatggc acatatcgca ggattagtag
    25921 cagcaggact tcatcctaat ccagttcctt atgcagatat cacaacgact accactcata
    25981 aaacattgcg tggccctcga ggcggcatga ttttgacaaa cgatgaagca ttagcaaaaa
    26041 aaataaatag tgcggtattt cctggtattc aaggcggccc tctagagcat gtgattgcgg
    26101 gtaaagcagt agcattcaaa gaagcattag atcctgcatt taaagaatat agtgaacaaa
    26161 tcatcgccaa tgcaaaagca atggtcaaag ttttcaatca agcaattgga accagagtca
    26221 tttcaggggc gacagataat catctgatgt tgatcgatgt tcgcgagtta ggtataaatg
    26281 gaaaagaagc agagagcatt ttggacagtg tgaatatcac tgtaaataaa aactcgattc
    26341 cttttgaaac actaagtcca ttcaaaacaa gcggtattcg aatcggcacg ccagcaatca
    26401 ctactcgcgg gttcaaagaa gaagatgctg taaaagtggc tgaactagtt gtcaaagcat
    26461 tacaagcaaa agatgataac gctcaactag acgaagtcaa aacaggtgta cgcgaattaa
    26521 ctgaaaaatt tccgttgcat aaaaaataaa aatttaaatt ccattagtaa acagaaaagt
    26581 tcaatgttgg gcttttctgt ttattttttg ggaaaacgga ttctttgaca atatcggtga
    26641 tgctctctag tatatgaggt gtaattttag tcttcacact aaatctcagg gaggaaaagt
    26701 catgactgat cgtatctcgt tagaaaagga agcagtagca ttcagtgatg caaatgcgcc
    26761 tcatccgcgt atctatgaac tagcaccgga agaaggaaga gttttactag aagaagtaca
    26821 agaatcacct gtgaacaaat attccgtcga tattgaagac ttgatggtgg atactggcga
    26881 atggggaaaa ctcaatattc gtctcgtacg accagaaggg aatatggata aattaccagt
    26941 tattttttat attcatggag caggctgggt ttttggcagt gctcaaaccc atgataaatt
    27001 gatcagagaa cttgcggttc gtacgaattc cgtcgttgtc tttccagaat atagtcgatc
    27061 accagaagca aaatatccaa cagcaattga acaaaattat gctgtgttgc aacagctgaa
    27121 taaattagcc aagtcaaaaa atctggatct cgagaatcta atcgttgcag gagattcagt
    27181 aggtggtaac atggctacag tcatgacgat cttaacaaaa gaacgaaagg gcctcccaat
    27241 atccaagcaa ttgctgtatt atccagtcac aaacgctgat tttgatacag attcctatca
    27301 tgaattcgga gaaaactatt ttttgacaag agaaggaatg atgtggtttt gggaccaata
    27361 tacgacgaat gaagaagagc gtgctgaaat cacagcttcg cctttgagag caaatataaa
    27421 tgaactaaat gatcttcctt cagcaatgat tttaacggga gaagcggatg tattaagaga
    27481 tgaaggtgag gcttatgcgc gcaaacttcg tgatgcaggg gttcctgtta cacaagtacg
    27541 cttccaagga atcatccatg attttgtcat ggtaaatgct atggatcaga caaaggcaac
    27601 gcgtgcagca atggcattat ctacttcttg gatcaagaaa aaagaataag ctttgcttgt
    27661 ccgcaggaaa acttcttttc aaaaacggat tttcttagga aaaatggcga gtttgactag
    27721 tcaatcactt gattttgttt tacaattaaa aagaaacgac agaacaaagg agagcttaaa
    27781 aaatgggcaa atttcaagtg atcgatcatc cattgattca acacaaacta actattattc
    27841 gggacaaaaa ctgcggaaca aaggtttttc gtgaagtagt cgatgaaatc gctatgttga
    27901 tggcatatga agtatcacgg gatatgccat tggaagacat cgtgatcgaa acaccgatca
    27961 ctgaaacaac acaaaaaacg ttgtctggta aaaaagtagc gattattcca atcttgcgcg
    28021 caggtattgg aatggttgac ggtatcttgg aattgattcc agctgctaaa gtgggacata
    28081 tcggtcttta tcgagatgaa gaaacattgg aaccacatga atatttcgtc aaacttccag
    28141 aagacattga tgcacgtcag ttgtttgtag tagatccaat gttggcaaca ggcggatcag
    28201 caatcatggc aatcgatgca ttgaaagaac gtggcgcgtc gaacattaaa tttgtttgtt
    28261 tggtagcagc acctgaggga gtaaaagctt tgcaagaagc gcatcctgat attgacatct
    28321 atacagcttc tctagatgaa aaattgaatg aacatggtta tatcgttcct ggactagggg
    28381 atgcgggtga ccgtttgttt ggtacaaaat aagtaaatac gaaaaagggt gtgacacaac
    28441 ttttgtcaca tccttttttg gtcattttta acgtttgatg cagaacagct ttttcacagc
    28501 tttcgtttgc atataaaaaa accatcccaa aaatgcagag atggtcaaat tcaaatcttt
    28561 tttagttctt tgtagttaac gattactaca cccttacttt gcgtcatcaa gttcgcattt
    28621 tctgcagttt catcaatctc aacaacagcg gaatttttca attgctttga aatcgttcca
    28681 gtttgcgact tgccgtgaat tgtgaacatt acgtgagtat tgatcttgaa tttttcacca
    28741 atttccgatg gtacgatagt tgtatcaaat tttccaaaag ttgccatttt cataaaacct
    28801 gcctttcaca tcaattatac cacgttttat gaaaaatatc atgaaattaa actaattttc
    28861 catcggactc tttacaatca gccaaataga gtatataatt tcttttggat aaggccagct
    28921 tagctcagtt ggtagagcaa cgcactcgta acgcgtaggt cgtaggttcg atccctatag
    28981 ctggcattaa aagagaagac agatatatca aggattttta atccattgat acatctgtct
    29041 ttttgtttaa aggaaataaa gtatgctttc ttagcaatag agcatgtatt atttctataa
    29101 aaatttagga gaataaaacg actagcaata tagctgtgaa atcaaatatc acatgaactg
    29161 cccaacttgt aatgatcgag ttgcttttaa tggcagcttg gttgaataac aatcttgcca
    29221 ccccttgtat caaaaggatg tgtgctacag tgtgtaaaat atttccatta tcataagtac
    29281 tgtaatggat aaatccaaag atgattgctg acaagaaact agcgacagga atgctgaact
    29341 tactagaaaa aatagcgaga aaatacactg agaacaattc ttctccaagt atcatgatag
    29401 gcaaaataat aaataataac gggctgaaag cctcatttat tggattgcct gttaaacccc
    29461 atttcaaaat ttgttgcaaa aataccgaga ctgcggcact gattactaca ttgataaaaa
    29521 aatatttggc tatatttttg acaggagcga caggtttcga gaacattttt ttcgtatctt
    29581 ttttaccaaa aacaagtgta aatgctataa atccagtgat agttaaagaa aaaatgctaa
    29641 taatggatgg cgcaaatagc aaccctaaca aaccgagtac aacgacaata gtgcccaata
    29701 tccctttttt aagttctatt ttttttccta aaatgttatt catgataata actcctacta
    29761 ttcagttttt gaagatagag tcagtataca tgaagagtta aaataggcaa tgttttttta
    29821 tggatatttg tattgctaaa aatttatctt atatattcat tttgattata ctacttttat
    29881 ttgtaaaaat tttctagcaa aaccaagtgc ttgcaactga ttaagcgtac aattttttag
    29941 atgttccgag cgtacttcaa tcatttcaaa ggagttgcta cttaaatcga tattttttaa
    30001 atcagtatca aaaaagctag cattttctaa atcattagta gaaaacatta ttttagtgag
    30061 ggaacagtct acaaaatctg cgcttgataa gacagagtgc tcaaaggtac tttcttttat
    30121 gtttgaacca gataaattta catagtccat tttacaattc tcgaataacg tgttcccaat
    30181 ataactttcc atcaatttag tacctgttaa tttacaattg acaaatttca cgcgatgcaa
    30241 catgcttttt gtcaatatac agcctgaaaa atcacagttt tcgaatatac agtcggtaat
    30301 ctccattctt tgaaaatcat tggaagcaaa aaatgcatac ttgaaaatag atttgtaaat
    30361 aattggatgg cagattattt ggttttcgat gacctctttt tgtgaaagga tattatacag
    30421 ttcaaaatct tcgttttcat agatatgaaa aaaggatcct ttattcaagc ttgcttcatt
    30481 aactttagga aaaatcatat ttaattacct ccattttcat aaagtcatat taacaaaagc
    30541 taaaccaact tacattagca gaaaatgaga taggttttcg cctttttcga taacgatagt
    30601 gtcgctatct cttgaaacgt tcttagaatt gccctgagag acacaaatta ggcgtaattc
    30661 taataaaaat cttagaataa aaatttttac tgatttaatt tagaatgttt ttaaaataaa
    30721 aatcgttgcc ataacagtgt attgaaaggt tttaacaatc cagtaatgat ttaaaaaaat
    30781 ctagatgtag cgtatacttt gtttatagaa tgtagtggga agaaatatat ttttgggagg
    30841 aaatcgtgat gtctaaaagt gaagaaatag aaaacatggt actaaaatta tatgatctcg
    30901 ttttaaatcc agagatcaaa gaaaaggaac gtatactttt gatagacgca aagacaggct
    30961 tagaaaaagg tcagtattat cctaaagtca taaataattt ggaacgtagt ttacgtccgt
    31021 tagcaatcag gggagaattg tctaaaccag tatcaccgtt ttatatggag atatcaacaa
    31081 taggaaaatt tgaaaaagaa ttaggtcgag gaatggcttc ggcaccgatt acttttgggc
    31141 atttataaaa acgagttatt catctcaatg aattaaaaag tatagtcaat ctcataatgc
    31201 aggctaggtt catttatttg aacagagtct gtattt
//
LOCUS       DAMEET010000027        31120 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.027, whole genome shotgun sequence.
ACCESSION   DAMEET010000027 DAMEET010000000
VERSION     DAMEET010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 31120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.027"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            126..1025
                     /locus_tag="PB780_001077"
     CDS             126..1025
                     /locus_tag="PB780_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDE0733145.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            1018..2280
                     /locus_tag="PB780_001078"
     CDS             1018..2280
                     /locus_tag="PB780_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDE0733146.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            2441..4339
                     /locus_tag="PB780_001079"
     CDS             2441..4339
                     /locus_tag="PB780_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HDE0733147.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(4638..5357)
                     /locus_tag="PB780_001080"
     CDS             complement(4638..5357)
                     /locus_tag="PB780_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDE0733148.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            5694..6026
                     /locus_tag="PB780_001081"
     CDS             5694..6026
                     /locus_tag="PB780_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733149.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            6013..8016
                     /locus_tag="PB780_001082"
     CDS             6013..8016
                     /locus_tag="PB780_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HDE0733150.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            8035..8505
                     /locus_tag="PB780_001083"
     CDS             8035..8505
                     /locus_tag="PB780_001083"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HDE0733151.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            8526..9110
                     /locus_tag="PB780_001084"
     CDS             8526..9110
                     /locus_tag="PB780_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HDE0733152.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            9125..10111
                     /locus_tag="PB780_001085"
     CDS             9125..10111
                     /locus_tag="PB780_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HDE0733153.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            10101..10412
                     /locus_tag="PB780_001086"
     CDS             10101..10412
                     /locus_tag="PB780_001086"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HDE0733154.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            10434..12215
                     /locus_tag="PB780_001087"
     CDS             10434..12215
                     /locus_tag="PB780_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HDE0733155.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            12208..13584
                     /locus_tag="PB780_001088"
     CDS             12208..13584
                     /locus_tag="PB780_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HDE0733156.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            13591..14226
                     /locus_tag="PB780_001089"
     CDS             13591..14226
                     /locus_tag="PB780_001089"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HDE0733157.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            14256..15611
                     /locus_tag="PB780_001090"
     CDS             14256..15611
                     /locus_tag="PB780_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDE0733158.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKITIHSKIALTVTGTLLLGGFVVFFFTERNGETLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(15803..17197)
                     /locus_tag="PB780_001091"
     CDS             complement(15803..17197)
                     /locus_tag="PB780_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDE0733159.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQKLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQNKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            17289..18260
                     /locus_tag="PB780_001092"
     CDS             17289..18260
                     /locus_tag="PB780_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HDE0733160.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKDKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSATLTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            18257..19038
                     /locus_tag="PB780_001093"
                     /pseudo
     CDS             18257..19038
                     /locus_tag="PB780_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
     gene            complement(19207..19578)
                     /locus_tag="PB780_001094"
     CDS             complement(19207..19578)
                     /locus_tag="PB780_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDE0733161.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TTPSYYFVIDPDGYKIEVIRG"
     gene            19712..20155
                     /locus_tag="PB780_001095"
     CDS             19712..20155
                     /locus_tag="PB780_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDE0733162.1"
                     /translation="MDKPIFGEKKQSANYQTRYAAYIVIERDKRIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(20198..21007)
                     /gene="yidA"
                     /locus_tag="PB780_001096"
     CDS             complement(20198..21007)
                     /gene="yidA"
                     /locus_tag="PB780_001096"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDE0733163.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(21026..22393)
                     /locus_tag="PB780_001097"
     CDS             complement(21026..22393)
                     /locus_tag="PB780_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDE0733164.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHKVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(22377..23207)
                     /locus_tag="PB780_001098"
     CDS             complement(22377..23207)
                     /locus_tag="PB780_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDE0733165.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            23370..23807
                     /locus_tag="PB780_001099"
     CDS             23370..23807
                     /locus_tag="PB780_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HDE0733166.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            23883..24503
                     /locus_tag="PB780_001100"
     CDS             23883..24503
                     /locus_tag="PB780_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HDE0733167.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     EKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            24692..25198
                     /locus_tag="PB780_001101"
     CDS             24692..25198
                     /locus_tag="PB780_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HDE0733168.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            25215..26114
                     /locus_tag="PB780_001102"
     CDS             25215..26114
                     /locus_tag="PB780_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDE0733169.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKARR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            26201..27086
                     /locus_tag="PB780_001103"
                     /pseudo
     CDS             26201..27086
                     /locus_tag="PB780_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     gene            complement(27278..27349)
                     /locus_tag="PB780_001104"
     tRNA            complement(27278..27349)
                     /locus_tag="PB780_001104"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27314..27316),aa:Arg,seq:ccg)
     gene            27409..28743
                     /gene="rpoN"
                     /locus_tag="PB780_001105"
     CDS             27409..28743
                     /gene="rpoN"
                     /locus_tag="PB780_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDE0733170.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            28892..29983
                     /locus_tag="PB780_001106"
     CDS             28892..29983
                     /locus_tag="PB780_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HDE0733171.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            30024..31025
                     /gene="gap"
                     /locus_tag="PB780_001107"
     CDS             30024..31025
                     /gene="gap"
                     /locus_tag="PB780_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDE0733172.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
BASE COUNT        10217 a         5419 c         6623 g         8861 t
ORIGIN      
        1 gaaagtaaag tgtcctatca ttttatgtac aacaaaaaga gcgtttacgt tttgttggta
       61 aataataaat cgcttacgtc tggtgtctaa ggatatatgt gatttcttga agaaggagtg
      121 aagaaatgtt agaagtcaag gatcttgtta aaacttttgg gaactacaca gctgtcgatc
      181 atgtctcttt ccaaatcccc gatggaaaaa tcatggggct aattggccaa aacggggctg
      241 ggaagacaac gacttttcgt ttgatcttgg attttttgac acaagaccaa ggagaagtgt
      301 tatggaatgg ccatcatctt ggggagaaag attatgatgt catcggttat cttccggagg
      361 aacggggact ttatccgaaa gtaacgattc aagaccaact gctttatttt gcagaactta
      421 gagggaaatc aagaaaagag attgaaccaa aaatcgattt ttggatggaa aaatttcagg
      481 taaaagggaa aaagacggat aaagtaaaat ctttgtctaa aggaaatcag caaaaagtcc
      541 agctgatcgc cacgttaatc catgaaccta aattgatcat tttggatgag ccattcagcg
      601 ggttagatcc ggtaaatgct gaattgctaa aagatggaat attggaattg aaaaaacatg
      661 gttcatgtgt cattttttct agccacaata tggataatgt agaaaaaatc tgcgatcatt
      721 tgatcatgtt gcgaaacgga aaaatggttt tgaatggaaa agtacatgag attcgtgaat
      781 catttggccg tacgaagtta ttcttggaat ctggtttaag ccaacaggaa gtagaagaaa
      841 tcgaaggcgt gaaaaaagtg atccttcatg aagacaatac gttagagatt accttagaag
      901 atcctgaagc tgggaaagaa atctttactc gtgcgactca gttcggctac attcctatgt
      961 ttaaccagca accgccaact ttggaagaga ttttcaaatt gaaagcaggt gaaatcgatg
     1021 cgtaagtttt ggattattac caaagatgtc tatttaaaaa atgttaagtc gatttctttc
     1081 ttgatcatga tattagtccc ttttatcatg ctgggtatca tttatgtagc aggaaacttt
     1141 gctcaacaaa atagtgatgt caataaaatc ggcgtattgg ctgataatga gcaaatcagt
     1201 cagcaaatga gccaagtcaa aacagatgat tttgagttca aagcagttga ttctgaagaa
     1261 aaagcacaaa aagagctttc ggaagaaaaa atcgatgcgt atttagtagt ctcaactgag
     1321 aatgataaga taaatgggat gctttatagc gaaaattcat taggacaaag tacacaacta
     1381 ctgatacaac aacaactgac tggcttgcaa tcgatgcttc gagcaagtca attaggcatt
     1441 tcaccagaag aagtagctag cttgagccaa caagcaagtt tgactcgaca aaaagtcaat
     1501 tttgattcaa aaggaaaaat gacagttgga gaagataact cgacagtcca atatgtcgta
     1561 agttatgtag caacgattgt cttgttcatt attattttga cgtatgcgca gatcattgca
     1621 caagagatcg cttctgaaaa aggcacgcgg atcatggaag tgatcctgtc cagtacgact
     1681 gcacaaaagc atttttacgg aaaactaaca ggtgttttac ttgttgcatt aacgcaaatg
     1741 gcattatacg gtattatttt tggtatagga tacagtcaat tcaaagatat ggaaatggtc
     1801 aaaaatatat tagacggtat ctcattggat aacttgattg gaccattctt gtggtattct
     1861 cttctattca tgatctttgg cattttgatt ttttctgttt tagcagcttt atgtggctct
     1921 ttggtcaaca aagcagaaga tacagcaaaa gcaattttgc cagtgactta tctatctcta
     1981 ggcggatata tgcttggatt gatactagga gcttcagatc caaacaatat cgttattcgg
     2041 atcacttcgt atattccatt cttatcttca tatattatgc cggttcgctt agcaaatgag
     2101 acagtcggta tgggtggagc attgatttcc ttgttggtac ttgtagccgt aacattctta
     2161 ttgatgatct tgtctgcaaa catgtacaaa tcgaatgtac tcgtgtatag cgaaggcgga
     2221 ttatggagtt cattgaaaca atcgatttca attatgagaa atgaacgaaa aaaagcataa
     2281 aatatagaaa aatatagaag gaagctggac atttattgtt cccagcttct tttttgtata
     2341 gacctatttc tattttttta ttatattgga tatatattca ttgaaaatag agcataacaa
     2401 ctttataatg ttgagtatac caatttttgg aggcgtttta atggaccgct atttagattt
     2461 actttcagag aagttcccta caacggaaga tgtcatcacg gaaatcatca atctcgaagc
     2521 aattttgcag cttccaaaag gaacagagtt atttttgagc gatatacatg gcgaatttcc
     2581 tgcttttgat catattctgc gtatcggttc aggaaatctt aaagaaaaga tcaaagacct
     2641 tttctctgaa caaatgacgg aaacggagat cagccaattc actattttta cggcttatcc
     2701 agaatatgtg ttgacgactg attggtacaa aaaacaagat aaatcccagc tgatccatcg
     2761 tttgatcgat cttttacggt ttacgacagt caaatacact cgatcaaaag ttcgaaaagg
     2821 attgccgaaa gaatatagct atatcattga ggaactcttg tatatcgatg atcgtatcaa
     2881 cgggaaaaaa gactatgtaa aaaaaatcat tgaacaactt gtcttgatga aggaagaaga
     2941 aaaattttta acaaaacttg cgcagacgat ccaaaaatct gtcatcgatc atttgcatat
     3001 cgtgggtgat atttttgatc gaggaactca agcagccaaa gtaatggatc gaatcatgga
     3061 tttttcctct gtagatatcc agtgggggaa tcatgatatt ttgtggattg gtgcgtattg
     3121 cggctctgag gcatgtttat tgacgctttt acggattgcc gcacgctaca actatttatt
     3181 tgaattagag aaagaatatg gattaaattt acgtccatta ttttcttttg cccatcaaac
     3241 ctatcagaag aatccagtat ttacccctaa aaacaggaaa gatctatctg aaagagagca
     3301 agaagaatta gaaaagatcc atcaagcact ttctatcctc caattcaaat cagaacatca
     3361 gttgttgaat cgacgacctg aatttaaaat ggacgatcgt aaattgttgg aaaaaatcaa
     3421 ctatgaggaa tcaacgatcg ttttagaagg tcagctctat gagttgaaag ggacttgttt
     3481 tcaaacgatc cagccagatg atcctaaaaa attcttggaa gaggaacaaa aagtgatcga
     3541 tagtttgatg tattcattcc agcactcgtt aagaatgcaa aaacacatga cgtttttgat
     3601 cgaaaaaggg ggaatgtatc tcacgaacaa tcagcatgta ctgttccatg gctgtatacc
     3661 tgtagatgaa aaggggcagc tcttagcatt tgaattagac caatcttatc gcggaaaaga
     3721 actgctggga ttttttgaaa aacagataac tgaaagcgca aaagatttga ctgcaaatga
     3781 agatttggcg accgatttga tttggtatgc gtggagcggg ccattttcgc ctttattcgg
     3841 caagagtaaa atggcaactt ttgagcgcta ttttctcaca gagcctcata cgcacgtcga
     3901 aaaaagcaat ccctattatc acttgcgaga tgaagagtgg ttcagtgaaa aaattttggc
     3961 agaattcgat gcaaaagaag aaggttcagc aatcatcaat ggacacacgc cagtaaaagt
     4021 aaaaaaagga gagtcaccga tcaaagcaaa cggaaaagca tttgttattg acggaggtct
     4081 atccaaagct tatcaaaaaa caaccgggat tgctggctac tcattactat gcaattctta
     4141 cggtttccaa atcgtgacac attatccgtt cttgcctatt gaacaacaat ttgcaaaaaa
     4201 atctgatcaa acgaatgtaa aaaaagtgat cgagcagcag ctcccacgca aacttaatcg
     4261 gaatacgaca aaaggaatgg aattacagca acagattact caactccaaa ggctgttgga
     4321 ttatcgaaag gacgattgag aaaaatgcta gccaacattg ctcaatagac taaaaagcga
     4381 ctgcaacaaa aggcgtgtcg cagtctcctt cggaattagc tcaaaaaacg gtaaaatatt
     4441 agaagctatt tttctgttta tttctctaat actcaggtca gggcgctgat ttcacgacct
     4501 cttccttaaa cggtgttctc ctgccaactc ctcggtcaca agtcacaata tccaacaaac
     4561 atgagaagct gttttttgaa attgctcctt gtgactcaga gttgaacggc aggctcacga
     4621 cctaatcaga aaaacgatta aaattcattc acaaagaaat cgaataaacg ctgttcagat
     4681 gaatctacac gatgagaaag ttccggatgc cattggacac caaggatttt gtgcatatta
     4741 tctttacttt ggactgcttc aatgagtcca tcgttggaaa aggcgatggc ttttaacaaa
     4801 ggagaaagat ctttgactgc ctgatgatga taggaattca caaagtatga atcagggaga
     4861 aggtcactta ataaactgtc cgaaacgatt ttgacttcgt gagttgcaaa ctgcggggca
     4921 gtgggctgtt gttcgtgttt tacggaccat tttggatata atgaaagatc ttgataaagt
     4981 gttccctcca acacgacatt caatagttga agtcctcggc agactgcaaa aatcggtttt
     5041 ctttgtttca atgcttccaa gatcaatgcc tgttcgaatt ggtctcgttg aatatttgtt
     5101 tccgtcagtt ttggatgagg gtcttccatg taaagttgcg gactaacatc ctgaccgcct
     5161 gcaagaatga gtttgtcgat tttctcaatt agttgaggcg ctgaatctgg agacatgact
     5221 ggcagaataa gaggtaatcc ttgagcatct aaaactgcat ccacaaatcc ctgcggtgta
     5281 tagcttactt gatttccttg gaaagtatct gtcgctcgaa tcagttgatt cccggcaatg
     5341 ccgatgattg gtttcatttt tattccccct aaagtagact aattgtaaca aggatttaaa
     5401 aaaatttaaa ctatgtaaga cttgccccta tacttatctg ttcttgctgt gttactatct
     5461 gtaaagagaa ttttcgtgtg aaattcatga cgatagttga aaatattgct taatttgtta
     5521 taataaaaag taagcagaaa taaaaaggtt gggcgtaagc ttcaaggatc tagttcttca
     5581 aggggtgaga aggacatttt attgaagggg cctgcctata ctaagttatc tttagaggta
     5641 catactttca atgaaaaaag aaagcatgta cctctttttt aaatcgaaag gaagtgaagc
     5701 aagtgttaga agctttggaa caaatccgaa aagcagaaga agaaaatgag aagcgtgctc
     5761 gaaccctacg gcagcagttg aagacctatg cagaagaaaa agagcaagaa ttggagataa
     5821 tccaaacgaa cagtcggcaa cagctgaaac agttggtaga agaaaaagaa caattcgaaa
     5881 agcaagaatt agaaaaagaa aaaatgaaat tgaaagcaga agcagacaag gtgcaagcaa
     5941 ctttgaaaga ccaatatcaa aagcatcaga gtcaagcaat cggtgcaatc attgaaaggg
     6001 tgaaagaaac ttatggccgt cactaagatg gaaaaagtga ccctcatttc agataaaaga
     6061 aatcaggaga ggattttaca agcagtacaa ggaatccaaa atgttgaaat ccgggatctg
     6121 tatcaaaata gaacaaacaa ccagtgggta gaaaaatatt ttccagatac cacgattatt
     6181 gataaagaag caaaattaag cgctttaggc aatcggctga aagacatcca agaagctatc
     6241 caattcatac aacaccacgg cgatagccgt caaaagaaac tccatttgaa acgtcaagaa
     6301 ctttccttac aagacttgga agcaaattat tccgaagaaa atttcatgaa taaattagaa
     6361 gaggtccttg atttaaaaga aaagtgggaa cagctaactg aaaaacgtga tcgtttagaa
     6421 gaagaggaag aatggcttct tcattggcaa cagctggata ttgctccaag tatgtatgca
     6481 agtaagacga ccatttttga gatggggact gtgagtgcag caaattttga aagtttcaag
     6541 gaagaactaa gtaagcttga tgacgtttat atggaagaaa tagattatac gccaaaacaa
     6601 gtttatcttt cgtatgttgc tttaaaaaaa tcagttgctg ctatagaaga aattgctggg
     6661 aaatacgggt tcaataaaga aacgtatcct tatgaacaaa atcctaaaga aaaattgcaa
     6721 gaaaccaaac agcaattatc cattgttcac gagcagcaaa aaaagataac gacagcgtta
     6781 ggtcagtgca gcggctatat ccaagaattt gaatgggtag aagaagtcac acttgccgtt
     6841 gcagaacggg aaaaagtaaa agaacggttt atccatgcca cttatctgat cgttttgcaa
     6901 ggttgggtag atgcagaaga aaaacagaat ttgatgcatg tgttaaacga aactgtatca
     6961 gaagaagata tatacgttag tttcgaaaca ccaaactcag aagagattca gcaggaagtc
     7021 cctactaaat tgaaaaacca tccgcttatc gaaccttttg aattgctgac tgaaatgtac
     7081 agtctgccaa aatatgaaga agtagatcca acgccttgga tgacaccatt ttatcttgtc
     7141 ttcttcggga tgatggtcgc agatgttggt tacggattgc tgatgctcat tgggacaata
     7201 ctagctcaaa aactatttgt tttaccccgc gggatgaaac gtttcgtaaa attctttgag
     7261 attctctcgg taccatcgat catctgggga ttgatctaca gttcattttt cggacaggca
     7321 ttaccaaaag aactatttgg tatccgcttg cctttcccaa tcctatctac gacggatgac
     7381 gtcaatacga ttttgatttt atccgtgatt ttcggtctga tacagatctt ggttggatta
     7441 ttcgtggcag caaaggaaaa tatcaggagg aaagattact tgagtgctgt cagtgatggt
     7501 tttgcctggc aagggatcct gattgggatc gttattgcat tgattggtgt catgttgata
     7561 aaaaatacag catttgttta tcttggcgcg gggctatcga tcattgctgc tttatgtatc
     7621 ttaatcgtgc cgattatcca atcgccgtct aaagtcaagg gtgctgccaa aggagcgtat
     7681 aatctttacg gattgactgg ttatatcggt gatttagtca gttatacacg tttgatggca
     7741 ctaggtattt caggcggaag tattggcgcg gcattcaata tgctagttgc tttcatgccg
     7801 ccagccgctc gattcagtgt agggatcctt ttgatcatcg ctttacacgc actgaatatg
     7861 tttttgacac tgttaagtgc ctatgtccat ggtgcacgat tgcagtatgt cgaatttttt
     7921 ggaaaatttt atacaggagg cggacgtgcc tttgacccgc tgaaaacggc ggaaaaatac
     7981 gtcaacatca atcataagaa aaaaaacaat aaataattgg aggattttta tacaatgatg
     8041 gattacttaa ttagtcaaaa tggtggaatg gtatttgcag tattagcgat ggcaacagcg
     8101 acgatttttt cagggattgg ttcagctaaa ggtgttggaa tgacaggtga ggcagcagca
     8161 gcgttaacaa caagtcaacc cgaaaaattc ggacaggctt tgattctgca attgcttcca
     8221 ggtacgcaag ggttgtatgg attcgttatc gcctttttga tttttatcaa cttaggaaat
     8281 gatatgtcag ttgtacaagg attgaacttt ctaggcgctt ctctgccgat tgcttttaca
     8341 ggtttgtttt caggtatcgc ccaagggaaa gttgcagcag caggtatcca aattttagcg
     8401 aaaaaacctg aacatgctac aaaaggaatc atttttgctg cgatggttga aacatatgcg
     8461 attttaggtt tcgttatttc cttcctatta gtattgaatg cctaagcata aaggaggaaa
     8521 agcagatgaa cgccattgaa aaaatcatct ctcagatgaa tgaagcagca gagcaagaaa
     8581 gagcggcgtt agagcaagaa gagcgaatga agatcgatca aaactttgaa caaaaacgta
     8641 cgcaaatcga aacagaacat caaaaacaaa aagaaaaaca aatcgaatta cttgaaaaaa
     8701 aataccgtca gcttaggaat cgccaacaag tagaagtacg acaagaaaat ttgaatgcga
     8761 agcaggaatt tttacgacga ttgtttgcag atgcaatagc tgaaatggag aattgggatg
     8821 aatctgaaca aatccagttt atcaaaaatt cattgtattc attacctttg acaggcaaag
     8881 taacattcat tgctggagaa aaatcagcag cttatctttc tcagacattg ctggatgaat
     8941 ggaacaatga acttcccttt atgatggttt taagtgatga aactgttgct gatcaagcgg
     9001 gttttctgat aaacgatcaa ggagttcagt ataattttct tttcagcagc cttgtacaag
     9061 atatccaagg aacgatgagt tttgagattg ccaatcaatt attcgaataa ggagggatca
     9121 gtgtatggaa taccacgaac taaatccctt gattcgcgga agagagcttg aacttttgtc
     9181 caatgagaca tttgaacgga tgattcaaac aaattctgtc gaagcacttg gtgaaatatt
     9241 gaaatccacg atttacagtc catatattta tgacggatat gaatatgact tcgaacagaa
     9301 tcttttaaag gagcagagcc gtctatttcg ctggttaaag gaatcagcac cggagcctga
     9361 aatcgtttgg atctatacga tgcgctatac tttccataat ctgaaggttt tgacaaaagc
     9421 cgagatgaca aatcaaaatc tggaccattt gtatatcgac gatggatttt attcattaga
     9481 aaccttgaag gatgccattc atacgcaagt ttcaaatgaa ctccctgaaa gcattatgga
     9541 cagtatacga gaagtacgtg attattttga agagtctacc attcttcagg gaattgacgt
     9601 gatttatgat cgacagtttt tgacagagca gcgacggctt gccgataagc taggatatca
     9661 agaattgctg acagaagtca ttgcttttat tgacttgacc aatatcacaa cgatggctcg
     9721 aggaatattg cagaaaagaa catatgggtt catgacgacg gtactttcca gctcgggaag
     9781 tatcgaaaaa gaagtatttt tgaactttgt agataaagat atggaagatt acacgaattt
     9841 tctattagag acagattatg ctgattttct gcgccctgtc atccgtgagg gaaatcttga
     9901 tctcgtaaag cttgaacagt tgaaagatga ttatctaagt tcattgtatc aagaagcaca
     9961 aacccaggct ttcggaccac tgccactact agcctttttg aacgcaaaag aggtagaaag
    10021 caaaaacctt cgtttactgg ttatcggcaa aagaagccat ttcgaaccag aaacaatcag
    10081 aaagcgggtg agacaagtct atgacgcata agatcggtgt agttggcgac aaagattctg
    10141 ttttgccatt caaattgttt ggctttgatg ttcgttatgg tacaaccaag caaaccgttc
    10201 gtcaggcaat tgaagaaatg gcaaagaatg agtatggcgt gatttatgtc acagagcaat
    10261 gtgcagcatt gagcccagaa acgatcgaac gttacaagga acaaatgata cctgctatcg
    10321 tattgattcc caatcatcaa ggatcattag ggatagggct ggaagaaatt caaaaaaacg
    10381 tagaaaaagc agtaggtcaa aatatcttat agtatgaaaa ggagatagaa attttgcaaa
    10441 ttggaaaaat cataaaagtc tccggacctc ttgtaatggc tgagaacatg tctgatgcca
    10501 gtatccaaga catgtgtcta gtgggagatc taggagttat cggagaaatt atcgagatgc
    10561 gtggcgatgt ggcgtctatt caagtatatg aagagacttc tggaattggt ccaggcgaac
    10621 cggttcgctc tactggtgaa gctttatctg ttgaattagg accagggatc atttcacaaa
    10681 tgtttgacgg aatccaacgg ccattagata cctttatgga aatcacacaa agtaatttct
    10741 tagggcgcgg agttcaattg cccgcattgg accatgagaa aaaatggtgg ttcgaaccaa
    10801 cagtagaagc cggagaaacg gtgagtgctg gagatgttat cgggatcgta gaagaaacaa
    10861 aagttataaa acataaaatc atggtgccaa atgggataaa aggtacgatc aaaacaatcg
    10921 aaaaaggttc attcactatt gatgaaacag tatgtgtcat tgaaaccgaa aatggtgaca
    10981 aaaatttgac gatgcttcaa aaatggccag taagacgtag tcggccaatc aaagaaaaat
    11041 taaacccgga tgcacccatg atcactggac agcgagtgat tgatacattc ttcccagtga
    11101 caaaaggagg agcagcagct gtacctggtc cttttggtgc agggaaaaca gtcgttcagc
    11161 atcaaattgc aaaatgggcc gatgtggaca tggttgtcta tgtgggatgt ggcgagcgtg
    11221 gaaatgagat gacggatgtt ttaaatgaat tccctgaatt gattgaccct aatacaggag
    11281 aatcgttgat ggaacgtacg gtattgattg ctaatacatc aaacatgcca gtagcagctc
    11341 gagaagcttc tatctatacg ggaatcacaa ttgcggaata tttccgtgac atgggttatg
    11401 acgttgctat catggcagac tccacttcac gatgggcaga agcacttcgt gagatgagcg
    11461 gacgtttgga agaaatgcct ggtgatgaag gttatccagc ttatcttggt tcacggcttg
    11521 cggaatacta tgaacgatct ggaagagtca ttgcattagg atcagaaggt cgcgaaggaa
    11581 gtataaccgc aatcagtgct gtatcgccgt ctggagggga tatttcggaa ccagtcacac
    11641 aaaatacatt gcgggtcgta aaagtattct ggggtcttga ttccagttta gcccaaaaaa
    11701 gacattttcc ttcgatcgat tggatacaaa gctattctct ttattcaaca gaagtaggga
    11761 agtatatgga tcatatcctt caagctgact gggcatccat ggtcaccgag ggcatgcgta
    11821 ttttgcagga agaacagcag ctgaatgaaa tcgtgcgttt ggtagggatt gattcccttt
    11881 cagataatga ccgtctgaca ttagaagtgg caaaatccat tcgtgaagat tatttgcagc
    11941 aaaatgcttt tgatgatgtc gatacattca cttcaagaga gaaacaattc aaaatgttga
    12001 agatgatctt gacctttggt gaagaaggac gccgtgcgtt gtcgttaggt gcttatttaa
    12061 gtgagatcat ggacggtacc gtatcgattc gtgaaaggat cggacgaagc aaatatatcc
    12121 cggaaacaga attagaaaaa ctagatcaaa ttattggtga aatcaagcaa acgatgcaaa
    12181 cgatcgtttc agaaggaggc atgagcaatg attaaagaat atcgtacgat cggtgaagtc
    12241 gtcggtccat tgatggcggt tgaaaaagtt gctggtgtca aatatgaaga attaatcgaa
    12301 gtccgtatgc aaaatggcga gattcgtcgt ggacaagtat tggaagtcca agaagataaa
    12361 gcaatggtcc aaatattcga aggaacaagt ggtatcaatt tacgtgactc ttctgtacga
    12421 tttttaggcc atcctttaga actaggtgta tcggaagaca tgatcggtcg agtattcgat
    12481 ggtttgggac gaccgaaaga taacggacca gagatcctgc ctgaaaagca tttggatatt
    12541 aacggagaag taatcaatcc agtagctcgg gattatccag atgagtttat ccaaacaggt
    12601 atctctgcaa tcgatcattt gaatacatta gttcgtgggc aaaaattacc agtattttct
    12661 ggctctggtc ttcctcataa agaattagct gctcagatcg cgcgacaagc aacggtactc
    12721 gattcatcag atgatttcgc cgtagtcttt gctgctattg gaatcacttt tgaagaagca
    12781 gaatttttca tggaagattt tcgtcaaacg ggcgcaatcg accgttctgt catgtttatg
    12841 aatttagcaa acgaccctgc aatcgagcgt atcgctactc ctcgtatggc tttgactgct
    12901 gcagaatatc ttgcttatga aaagggaatg catgttttgg ttatcatgac agacatgacc
    12961 aactatgcag aggcattgcg agagatttct gcagcacggc gagaagttcc aggaagaaga
    13021 ggatatcctg ggtatcttta cacgaactta gctactttat tcgaacgagc aggaagaatc
    13081 cgcggactaa aaggatcggt tactcaaatt ccgattctaa caatgccaga agacgataaa
    13141 actcatccaa tccctgactt gactggttat attacagaag gacagatcat cttgactaga
    13201 gagctttaca agagtgggat tcagccacca attgatgtac tcccgtctct ttcgcggtta
    13261 aaagataagg gaactggacc cggaaagaca agagaagacc atgcagctac gatgaatcag
    13321 ctttttgcag cctatgcaca aggaaaacag gcaaaagaat tggccgtagt tttaggtgaa
    13381 tctgctttat ctgatgtaga caagatctat gcgaagtttg ctgagcggtt tgaaaaagaa
    13441 tatgtcaatc aaggattcta cacgaaccgt tcgattactg aaacacttga tctagggtgg
    13501 gaactgctat cgatgttgcc aagaaccgaa ttaaaacgaa tcaaagacga catgctggac
    13561 aaatatctgc cagaagggaa gtgagttctg atggcacgat tgaatgtgaa tccgacccgc
    13621 atggaattga ctcgattgaa aaaacagttg acaactgcca caagagggca taaattatta
    13681 aaagataagc aagatgaact gatgcgtcaa ttcatcctgt tgattcgaaa gaacaaccag
    13741 ctgcgtcaag aaatggaaga gtctatgaca gaagcaatgt ctgattttgt attggcaaat
    13801 gcttcgatca cggagccttt catcgaagaa ttatttgttt tgccggcgga aaacgttgat
    13861 ttgtctatcg tagacaaaaa tatcatgagt gtaaaagtac ctgtgatgaa ttttgaatat
    13921 gatgaacagc tgacagacac acctttggaa tacgggtatt tgaattccag tgccgaactg
    13981 gatcggtcaa tcgatcggtt tactaccctg cttccaaaac ttttggaact gacagaaatc
    14041 gaaaaaactt gtcaactgat ggcgagtgaa atcgaaaaga cacgtcggcg agtcaatgca
    14101 ttagaatata tgacgatccc tcaattagaa gaaacgatct attatattcg tatgaagctg
    14161 gaagaaaacg agcgcgcaga agtaacaagg ttgatcaaag taaaaaatat gggtaccact
    14221 gactaagtgg tatccttttt taaaagagga aaacgatgat taaacaaagc aaacaaataa
    14281 agaaacgctt gccggctgtt cagcttctcg cattaggatt ttttctcctg attttagttg
    14341 gcggcggcct acttacccta ccatttttta gtaatagtgg gcatgggaca aattttatcg
    14401 atgcgctttt cactgcaact tctgctgtct gtgtgacggg attgacaaca ttgaatacag
    14461 cagagcattg gaacagtgct ggtcaatttt tgatcatgct tttgattgag atcggcggtc
    14521 ttggttttat gatggttccg attctatgct ttgcattgat aaagaagaaa gtcagtctca
    14581 gcacacgtat tatattaaaa gaagcgctga atctagaaga agtatcaggt gtgatgaagc
    14641 tcatgcttta catcctgaaa ttggcctttg tgatccaact gattggtacg gttgctttat
    14701 cttttgtatt cgtgcctgaa tttggctggt tgaaaggtat ctggtacagt ttgttccatg
    14761 caatctctag tttttgtaat gcagggtttg atttgttagg agacagttta gccaatcacc
    14821 agcaaaatgt ttatttgatc atggtggtat ctgctttgat catttctggt gggttaggct
    14881 tcatcgtatg gcgtgattta ctggaatatc atcagaaaaa gaagataacg attcattcaa
    14941 aaatcgcatt aaccgttaca ggtacccttt tattaggcgg attcgttgtg ttctttttca
    15001 cagagcgcaa tggggaaacg ctagtggaag ggaatatgtt tcaacgattg gcaaacacct
    15061 tttttatgag tgtgacacct agaacggctg gatattattc agttgattac ctccatatga
    15121 gccatgcagg tctgatttta acgatgtttt tgatgtacat cggtggaaca tctgggtcga
    15181 cggccggcgg attgaaaaca actacgttag gtgtgttgct gatccagatg cgttcgatgc
    15241 ttaaaggacg tacgagagcg gaagcatttg gccgtaccat ccgacaaggc gcagtgttgc
    15301 gggcacttac tttatttttt gttacgttga cgttgtgtgt agtcgccatc atgattttat
    15361 cggtgactga aacgatacct aaaacttctg gcatcgaata tattgctttt gaggtcttct
    15421 ctgcgtttgg tacagtaggt cttacgatgg gattgacgcc agacttgact gcaataggga
    15481 aattgatcat tatagcgctt atgtacattg gacgagttgg tgtattgact gtggtcttct
    15541 ctctgatgac aaaagcacaa agaacacaat caaaatacaa atatccagaa gaaagtgtca
    15601 tgattggata atttcatctt ctgacagaaa cctagtgatt tagtaaaata agaaaataaa
    15661 ataatttttc ttgcttcttg gatactttaa gtgcaaaatg ttcgtttatt ttttgaggca
    15721 ctatccgttg ttgaacctcg ggtagtgcct ctttcttatt gaaaggaatc aataagattt
    15781 gattccttta tttatcaata ggttataatt catatttttt tattttttct tttacgatac
    15841 ttagtccaga agctaaaatt tcaaggttat tagaggatag ggaaattcga acgaatgcat
    15901 ctggttgcgc acgtgtagta aagcgtctgg aatgataaag tcggataccg ttagcaagaa
    15961 agtcattctc aattatctta ggatcaatct caggatcaac tggaagaatt cgatagtaag
    16021 ggacacgatt cgttggtttt tctactttga aaagagaatc aaaaagctga ttggctcttt
    16081 tcgtaagact taatttagtt tctaagatcc tatttgcttc atttgaatgg aggatttgag
    16141 tgatgacttc cacatctaat ccagaagttt tcacatttat attaaaaatt gcttgttcta
    16201 tcttgttttg gacatgtact ggaactacaa gataagcaac tcgcaatcca gaacaaataa
    16261 attttgtcat tccagctaag tagattgttt ggttaggtaa taatttttgg aaaggaggaa
    16321 tgatcgtatg ttgatagtaa gttgttaaaa atgaatggat atcatcttca ataacccata
    16381 gaccttcttt ttcaatcact tcggctaatt gctctcgtct tttgtgagag atttgaaacc
    16441 ctataggatt attacaagaa ggcataagaa agacaccatg aatcttcttt tttctacatt
    16501 cctgaatcaa tatatcaggt cgcattcctt gctcgtcaaa atcaattgga acgatctcta
    16561 aatgatataa ctgagccaat tcaatgaaat tagcgtatgt atatcggtct gttgctattc
    16621 tatcacctgg agagaaaagt gcagaaagtg tgatagccaa tccattttga acaccggaaa
    16681 caatagatag agattctaag gacggaatga ctccttgagt ttttaaccaa tttgctgcag
    16741 tagtcaattg aaaatttgtg cctttgggat cttggtaatt aagcaaacca acaagatttg
    16801 ggttattact agttgattga ataaacggga caacgaattt attacattct tcaaaagaac
    16861 taaccagacc aaaatcaatg atatgttcgg ttacttcatt ggtagagatg gtgatggact
    16921 caaagacatt gggagaaata aatgtgccac ttccaatatt cgtatacagc aaacctttct
    16981 caattccata tttataagcc ttgccaacgg tagtaaaatt taaatctaaa taatcagcta
    17041 gctctctttg ggagggtaac tgagtgtttt tctgtaattt attctttaaa atatcttgtt
    17101 ctaattgttg aatcaacgat cgataataag gacgcttcaa tttttgtttg tctggtcgcc
    17161 aagttaacgg gtaattgtca taagaattaa taggcatttt ctacactcct taaaattgta
    17221 taccatacaa ttttaccatt gattgtattg attttagaag ttaatctttt gtgaggaggg
    17281 aattgtctat gaaaattatt aattttgagg aagtagtagc ttcttttagt tttgaagaag
    17341 cgattcaagt aatgaaaaaa tgtttttttg attatgaaag tggtaaaatc agccaaaatc
    17401 ctcgattggt ggaaatttta cctgacggaa aagacaaaaa tatgtttgcg atgatgcctg
    17461 cttatttggg ggagaacaga tattttggaa caaaagtcat taccgctttt ccggataatc
    17521 atcagtatca tttaccttct catttaggag agattttctt gtttgatgct caaaacggac
    17581 tacccaaagc attgataaac gcaaatgcag tcacctggat tcgaacagct gctgtatctg
    17641 cgctggcaac agattactta gcaaatccgc atgcagaaac acttgcttta attggagctg
    17701 gtcaacaagc attgtcgcat ttgactgcga tttctaccat tcgtcccatc actacagtgt
    17761 ttgtttacga tattttagaa gaaagacgaa atacttttat cgctaaagct aaaaaacagt
    17821 atcctaaaat aacgtttata aatgcaatat cagtagaaga tgcagtacgt ccagcggaaa
    17881 tcatttgtac gttgacctct agtaaagagg catttcttca aaaagagtgg gtaagggaag
    17941 acgcacatat caatgctgtg ggcacattta ctcctgatac aagagagata agtacatcgt
    18001 tgatggaaac ttctcaaatc tatgtagatg attactcagc taccttaaca gaaagcgggg
    18061 acctgcttat agccatggca gaaggagatt tagaggaatc ttgtattgtt ggtacattag
    18121 gcgaattagt ttctgagaaa aaaacatttt ataaaggaga atcgaggccg acgattttcg
    18181 acgcagttgg tctagctatt gaagatcttt gttgtgcaga atatatttat aacaaattag
    18241 agaaaggggc tttaaaatga acttagatta ttacgtagtt gatgcatttg cagatgaggt
    18301 gttcaaagga aatccagcag cggtttatgt gctagaagaa tggctgccag aaggtacgat
    18361 gcaaaaaatt gccattgaaa acaatttatc tgaaacagca tttacagtga agaaaaacca
    18421 agaatttgag cttagatggt ttacgccaga tcgagaaatc gatctttgtg gtcacgcgac
    18481 attagcaaca gcatttgttt tatttaacta ttacaaaata cctgatgaaa cgattaaatt
    18541 ttctactcaa agcggtaatc tatttgtgac aagaattaaa gattattatt acatggattt
    18601 tcctagtatt atgccgaaaa aagtgccgat attgactgaa tatgaagaag caattggggc
    18661 taagattaaa gaagcctact tagcacgtga tttatttttc gtattagaag atgaaaaaac
    18721 ggtggctaaa ttgcagccag attttacagc tatcaaaaat tttgaattag gaatagagtc
    18781 atcgtgacag cagaaagtgt gcaaaaagac tttgtttcta gaaccttctt tccaaaatta
    18841 acaatcaatg aagatccagt atgtggatca gcacattcta atcttattcc ttattgggca
    18901 gaaagaatga ataaaacaaa attgtcagct tatcaaatgt catcaagggg cggggatcta
    18961 gaatgtgaat taaaagaaga tcgtgtgata ataggaggaa aagcagtttt gttttctaaa
    19021 ggtacctcgt ttatttgatt tctttcaaat gaattaaaaa taaattggtg gagagtggca
    19081 gccttatagc tacaatggtt gttaaaaaaa gacgcaaact ttttcaattt aatgaaaagt
    19141 ttgcgtcttt ttttaattat tggtgtttgt tttttattca atcaagagta aaatgcccca
    19201 tactaactat cctcggatga cttcgatttt ataaccatca gggtcaatga caaagtagta
    19261 agaaggcgta gtgccaggta atccttttaa gtcagtaatc tcaagacctg cagctttatg
    19321 ttgctcgtgt aatttttcta aatcatcagc tgcaatagcg atatgaccat agccgtctcc
    19381 tagatcatac gcaccatgat catagttgta agtcaactct aattcatagt catctccagg
    19441 aagtgtcaaa taaacaagtg taaatttgtt ttctggaaaa tcacggcggc ggctttcttc
    19501 aaaaccaaat gccttttgat aaaattcgat tgatgcttca agatctttca cccgaacaca
    19561 tgtgtgagcc attttcataa attcatctct ccttttcctt ttactcaatc ataacaaaaa
    19621 gaagtaagaa aatagttgat ttgtgcttgt tcatgagaaa tttctcatgt aaaatggata
    19681 gaaaataata ctgttaaggt ggaaacaatt catggataaa ccaatttttg gtgaaaaaaa
    19741 gcaatctgct aattatcaaa cccgttatgc agcatatatt gtcatagaaa gagacaagcg
    19801 gattgcggtg atcgaggctc caaatggtgc ttttttcctt cctggtgggg aaatcgaagg
    19861 agaagagacg aaagaagaag cgattgtccg tgagctgata gaagaaatgg gaattgtggc
    19921 agaaatttct tcttatctgg gagaagctga tgagtatttt tactcgaatt atcgtgcaac
    19981 ttattattat aatccaggat atttctttgt tgctgattct tggaagcgta taggagaacc
    20041 aactgaaaag accaataaaa tttggtgggt gagccctcaa gaagcgcttg tgaaattgaa
    20101 acgtggtagc caccgatggg cagtcgagag atggatcgaa aaaaataaaa actgaaagaa
    20161 agcgagtaga tcagctttct ctcagttttt ttatttatta cagtacatat tttttgattg
    20221 cttcagctac accgtgctcg tcgtttgatg aagtgatgac atttgctgct tctttgacaa
    20281 gagaaacggc atttcccatt gctactccta gaccagcata ttcaatcatg gataagtcgt
    20341 tctcgttatc tccgatagcc atgatttcat cttgatcaat accaaggtgc tgtgcgagat
    20401 tcgcaactgc cgcaccttta cttgcttctt tatttagtac ttcgaagtaa tagggcgcac
    20461 ttttgaccgt tgtgtattta tcacggaaag tttgcggtaa acgcgcaatt gctgcatcta
    20521 aaatttctgg ttcgtcgatc atcatcattt tgacgatgtt catgtctggt gtcatttctt
    20581 caggtgttcg atattttagc ggcatcttga ctaaacttgc ttcgtggatc gtataagcgc
    20641 tgatatctcg attaggcgtg tagatcgctt tgtcatcgat cgaatggaga tgactgccga
    20701 cacggcgtgc catcacttcg atctctagga aatcttcatg agtcagtccg tgacgggcga
    20761 tgattttacc tgatttcgtt tgttgtacga gtgccccgtt ataagtaata acgtaatcat
    20821 tatcttgaaa aaggttcaac tcgtttaact gttcctggac acctggtaag ggacggccag
    20881 tgcataaaac gatcgaaaca ccttttgcaa cagcagcggc aatcgcttct tttacttctt
    20941 ttgttacttc tcttttctca tttaataaag taccatcgat gtcgatagcg actagtttga
    21001 tagacataat ttcctccaat atattttaat ttatttcaat caatgcgcca ttacggatat
    21061 ggctggcaaa ttcttgataa gtttcgtcaa ataaatcata atgatcacgc aaggagggat
    21121 caaccatttc ttttggaaag aaaaatcgct catctccttg ttcttgtccc gctaatgcag
    21181 caacaagctg actaacctgt gataattcta tcaaagttcc gtcatttcgc aaaatctcga
    21241 tttgtgtgcg atgtctgcct tgttctgggc gataaaaatc ataaggcaga tcgtagctag
    21301 aattcacagc agtgtaataa acagggttat agccaacctt ttctactaac aaagtgagtt
    21361 cttggactaa ttttgaatcc cggttgccag aaaaagtagc agatttcagt ggtttacgat
    21421 tcaaaaaacg tttagctaaa tcatttaaaa taggatcagc agattcagac cattgagtaa
    21481 agtaagtgcc caagacccca tcatcaagtt tgaggtattc ttctaaagta aagcttccgt
    21541 caagaaatgg cagaagtaat tgagaatgga gttcaaaact gctggcatct tcttgataca
    21601 actcgtgtgc gcgatgaagc agatgctcca aaatcacttc cataccacgg gaaacaggat
    21661 ggaagtacac ttgcacatac atttgatatc ggctaacgat ataatcttcc actgcatgca
    21721 tgccactcat ggaaaaagcg atcccgcctt cataaggacg aatcacacgt aatattcggg
    21781 taagatcaaa tgttccatat tctgttccag tgaaataagc atcccgaagc aaatagtcca
    21841 ttcgatctgc atcgatctga ctagaaatca tttggacaac ttgtgggttg gggtaagttt
    21901 tttggatcac actagctacc ttttcaggaa aaccttcttc tactcggtta agaatctgat
    21961 acacttctgt ttctggagaa gtgatgatct ctacagtgat ggcttcatga ttcgtatgga
    22021 agatatgttc aaatgtatga gagtaaggac catgaccgac atcatgcaac agtgccgcac
    22081 ataaagtcac cagccgttcc tgatcatccc agcctccgtt gcctattttt tcttttgaaa
    22141 aatttcgaga aaagatatcg cagattcgtc ttgaaatctc ataaacgcct aatgaatggg
    22201 taaaacggct atgttctgct ccgtgaaaag tgaaagaaga agtgcctaat tgtttaatgc
    22261 gtcgtaaacg ttggacttct ttcgaattga tcaaatctaa gattacttta tgctggacat
    22321 ggatatagtt atggactgga tcacgaaaaa ctttttccat aggcagcatt tgatatttat
    22381 aaggggttgt cataatgtag ctcctttatg ttattacgtt gttccattct ctggatttgg
    22441 ttgttccatt gctccagctg gatttctttt tttagccagt cggtagaatc ctgtaaatca
    22501 cttgcagaat acagcttttc aaaagctttt gcaaatgctt ctttgacttc ttttacaaaa
    22561 aggggttggt caagcagttc ttcaagtgtt gccattgtag caggatccac tgcaggatag
    22621 ccatttgtac caaagtcttc ttttaaagca gattgataaa atcgcttcat gacttgcccg
    22681 cgtttttcct gattaccgtt gacacttaag tacatcataa cagaaatccc atttttgatc
    22741 cgacgctgag cgataccggc aaattttttg ccatggatgc ttaaatcata agttccagga
    22801 caataagaat gggtgatttc gaatgcttgt atcggtgcct ttggaaaagc gtatttagtc
    22861 aataggagca tttgctcgta agcgtcatct atggttattt tttttgtgtc actttgaggg
    22921 aaaatcaaag aaatatttaa aattccttca tctgatacga tccctaatcc acctgaattt
    22981 ctgatcactg gagcgtaacc tgcatctttc agtgtttgga tcccgtcact tagaaaagga
    23041 gttcgaacat ccttcatacc taagatgact gtgcagtcat attgccagaa atgtaaaaag
    23101 atttgctggt gttctttaga atattccgtt aatatatctg ttaatgcaaa aggggaaaag
    23161 tcgttttttt gataaatgtg ctgatctagt cgaatcgcag ggttcattca accggctcct
    23221 ttgtataaat tcatattttc tacaatatta tactctaaaa tcaaagagtt gttttggaaa
    23281 aaaggttttc tgtaaaaatg gacaagttgg ttgacaaaac aaaagaaact aggaacaatg
    23341 tagctatgag aagtaaagga gaatgaataa tggaattatc acaaggaaca ccgatttcaa
    23401 tcaaattgcg tacaaaagta aaacagcaag gggaagtgca agatttttat tttgatttga
    23461 aagggcaaat ggtgacaatt ggcgatacgc tgtacatacg ttataaagag attcaggagg
    23521 agacgggcga tgagattcct gtaacgatca aattagttcc agatggtcat gtacagctga
    23581 tccgtgcagg agaaatgcgg atgcgtttga aatttggcta taaagaacgg ttagaaacat
    23641 cctatcgaac accatacggt atgatccaga ttgctacttt tacgaaagaa ctgcatgtta
    23701 gtttgaaaga ccgtcccaca gcagggaagg ttcgaatcga ttatgatctg ttcatgggac
    23761 cggaaaaaat cggcgaatat tatttgacct tggattttac agcgtaaata taaaagaaaa
    23821 gtttgatttt ttgtgtctga ttttgtatga taaggagtag actgtgaaag gacgtgtcct
    23881 gattggaaat tagtgtattt gaaggtgcaa acaaaagcga attatcaatg atcgaagtag
    23941 cacacgcgat cttagaacaa cgtggcgatg tcatggattt ttctgattta gctaaccaga
    24001 tccaaaacta tcttgagaaa tcagacagtg aaattcgtga ttcactcgca caattttata
    24061 ctgacttgaa tattgacggc agctttatct cattagggga taatcgttgg ggcttgcgtt
    24121 catggtatgc tatcgattcg atcgatgaag aagtaaacca tggaatggac gaagaggatg
    24181 aagatactcc acgtcgccgt aaacgcaaga aagtcaacgc ctttatcaat gatgatgaag
    24241 acgcgatcga ttacaatgac gatgacccag aagatacaga tctgacagaa gaagatgacg
    24301 atgatctgtt tgatgacgat gatgatgaag atgaagaaat cgctgcttat aattctgact
    24361 tgcaagaaat cggagcagat gacccgacag atgatgaaga agacttgcca ggaaatatcg
    24421 aagaagattt aagtatcata gacgaagacg atgatgatga agattatgaa gaggaagaat
    24481 tttctgactc tgaaaaagaa taaacattat tttcttacga gaggttgtga cagagacgat
    24541 cagcctatca gaataaggag gtgtgacacg atttttgtca caccttcttt tttgtatata
    24601 aaaatcccaa gtaaatacat aaaaaataaa aaaattgaaa cttttagcgc accttgtacg
    24661 tcatattaag tgaggcagaa aggagtggag aatgattttt actggatata agaaaaaacg
    24721 gcagatcgaa aaataccgtc acctgctgaa tggactcatc gaaactgatt atcaaaaaat
    24781 gttccgtatc acgatgaatt acgtacatga taaagaagag gccttagatg tgatgcaaga
    24841 tagttttcat aaagccttga actattttga aaaagaaaaa gagatcgagc attttagtgc
    24901 atggttttat cggatattga tccgcacggc attagatagt tggcgcagac aaaaaaggac
    24961 gcctgtggac ttatttgaaa ttcaagatat cccccaacag ttacctttag aaacttccgt
    25021 tgcagagctc catatgattt tagataaaat agacagtccg gaaaaagaga ttctgatcct
    25081 ctactttttt gaaggatttc gtttgaaaga aattgcaact atcttggata tgaatgaaaa
    25141 cacagttaaa acgaaaatgt accgctcatt gaatgcttta cgtcaaatcc tagaatgaag
    25201 gagagaagag tgccatgtat tcccaaaaag aaaatttaaa aaaactaaag aaaacttata
    25261 aagaacaagc aattcccaaa gggcttaaag aggagatccg ccagcgtttt attatcgaag
    25321 agcaatcatt tataaaagca aggcggagaa agagacgtct ccaagggttc ggagtatctt
    25381 tggctatcgt aatgattaca acaagtagcc ttctttttaa tagtcaagtt cgaagctttg
    25441 cagaagattt acccatctta ggatcagtca ttgaattgat cttaggcgaa cgctttactg
    25501 atcgatctga aaaaatcgat attcaggtac cgaagatcaa tacacaaaat gagaaagaaa
    25561 ataaaacaat tcatggatta aatcaaaaat actttcgcga aggacaagca gattttgaaa
    25621 aagctgaaaa agaatatgga aattttgaaa cggatcatta ccaagttttg ggagattatc
    25681 agaaaatcgt agacgataac cggtttctcg tgattgaaag gcagatcacc caaacagcag
    25741 cagatagcca tgttgaaaaa agatacgaca caatcgacaa gaaaaacagt gtccagctct
    25801 cacttccact tttattcaaa gatgacactt atctatctgt tttgacaaaa gaagtcaaac
    25861 ggcagatggc cgaacaagtc aaggaagatc catctaagta ctattggact gagcaagata
    25921 ttcaagaagg gacgatagaa aaaccaacac ttgttacgcc tacacgaagt ttttatttga
    25981 acaaagagca tcaattagtt ttgacttttt ctcaatacga aattgctcct ggttatatgg
    26041 gtacaccaga atttgtcatt cctaagtccg tgacaaaaac gattttggca tcggaagatt
    26101 atttggatca ttagacgaga agtagttgcg actagcagtt tcaattttca agtaagtaca
    26161 agtaaataat aagaagagaa aaagagaaag aagagatata gtgaaaagaa attatttagt
    26221 catcagtatt attgctttat ccgtgctttt gtttttcgaa attacttcgg tggcctcctt
    26281 gatcaccgaa gtaaaaggac aaacacactc ttcggagcat tcgtcacaat ctttcaaaca
    26341 atcagaacaa tcaaccatta aaagccaatc aactgaaaca gctaacgtca aagaagccat
    26401 tcaagaatca gcggaaaagg tacaaagtca atcatcacaa tcaacacaag acagtacaga
    26461 agcaatccaa gacccgactg tcaaaagcgt cgcaatcagc tttgatgatg gaccaggagc
    26521 gaccacaacg ccgcagcttc tgcgaatctt aaaagaaaaa aacgtgcatg tcactttttt
    26581 cgtcttagga gaaaatacgg cgcagcatcc tgaaatcgtg aagcagacag ctgaagcagg
    26641 gcacgagatt ggcaatcaca catacgatca tcagaatctg gccactcttt ctgcccagtc
    26701 gatgacggag gaagtgacga aagcagacac tgagatcaaa aaaagtcact ggcaaaaccc
    26761 ccgcttttgt tcgtccgccc tatggttctg tcacgaatgt aggagcaacg atcatccagc
    26821 gaccgatcat cgaatggtct gttgattcag aagattggaa gacaaggaat cctgatttga
    26881 ttttgcagaa gatccaagca acagtatacg atgggtcaat tattttattc catgatatct
    26941 atcctgaaac gattcgtgcc gttcctcaag tgatcgatta tctgaaagag caaggctatc
    27001 gcatcacgac agttagcgat ttattagggc atccaacggc tgtcgagaac tattatggca
    27061 gaaacgatca tcggccagtt cagtaagtga gacaagtatc tttaataaat aacacatttt
    27121 aataagtagt tgaacacata actaccttac ttgcaaaggc ttaacaatat ctatacagat
    27181 acaacaaaaa actgtccacc aattctcttt ggcggacagt tttcacttaa aaataaagaa
    27241 gcaactattc gtaaattatg ctaaaatagc agtagaacgc gtcctgaggg aatcgaaccc
    27301 ccgtctcaag aaccggaatc ttacgtgata tccactacac taaggacgca agcactggga
    27361 taattgtaac taaacagata aaaaattaca aggggattgg acaaaaaaat gaaatttgag
    27421 caaaattttt cacaaaaaca gcagcagaca cagaaactag ccatgacgca acagttgcaa
    27481 cagtccatcc aagttttaca atataacagt gaggagctgt tggcattcgt tgagaatcaa
    27541 gcaatggaaa atccattggt ggaagttgtt gagccggaat ggcagccaga ttacataaaa
    27601 gcttcttcat cttcttatga aggagaagag acaaattacc taaatcagat tcctgataca
    27661 aaagggtctt tatttgattc attgatcgaa caagtccatt tgaattaccg agatactttt
    27721 ttgaggaaaa tcgttcttta tcttgtagaa tacatcgatt tgaacggatt tttgacgatt
    27781 agtttagaag aagccggaaa agagattgga gctacgccta tccaaatgct cgatgcgttg
    27841 actctgattc agcaattaga accagctgga gtaggtgcac gtagtttgca agaatgtctc
    27901 atgcttcaaa cagaacgaga tgattatgcg ccggaattag cttatatcgt gttggaagaa
    27961 tgttttgagg aactggtgga gcgaaaatgg aaagaaatcg cacaacgttt ttctgttgat
    28021 ctacatgctg ttcagcagat attcgactat ttacaaacgt tgagtccaag ccctggccgg
    28081 attttcgacc gtagcagcga attgtacatt attcctgatg tccgggtatt agtggacgac
    28141 gataaaaatg tacaagttat ctcaaaccga aaaaatcagc caaatatacg ctttcaagag
    28201 agttacttca aacaaatgtc acaacaagca gataaagaaa ctgaaaatta tttaaaagaa
    28261 cggaaacagg aattcgaatg gttgaagaaa acgatcctcc aacgtgggga tacaattttg
    28321 cgggttgctc aagtcattgt ttcccggcaa aaagctttct ttatcgacaa ggaacgtcca
    28381 atcaagcctc ttacgttaaa ggaagttgca actgagatag atgtgcatga atccacggtc
    28441 agtcgtgcag tcaacgggaa atatttggaa acagatttcg gtgtatttga attaaaaaaa
    28501 tttttcacta cacgaattcc tacgaatagt acagaacaga cagaagacct ttcagcagat
    28561 acagcgaaga aaaagctgca ggaattagtc gatcaagaag acaaaaacaa gccgttgtcg
    28621 gaccagaaat tggttgaatt gctgaaaaaa gatgagatag cgatctcaag acgaacagta
    28681 gcaaaatatc gtgatttatt aggaatcccg agttcatcaa aaagaaaacg ttatgataac
    28741 taatcagtca aaagactaga aaaagtattt cactttttct agtcttttta gttgaaaaac
    28801 gagagcaaaa ctgttagact tacagtcgtg aggttgagat ttccctttat tgatgtgaaa
    28861 ttcgaatagt taatttcttt ttttatcgta tatgggtcgt tttaagtcta cattggacgt
    28921 aaagtgtcca actaaggaga tcgctatgct ggatgaaata aaaatgattg aatctgttgc
    28981 accagatatt atcgaagtgt tgcaagaacg ctataaaatt ttacgaaata tctattggat
    29041 gcagccgatt gggcgtcgaa gtttatcgga aagtatgggg atcacagaac gagtcttacg
    29101 aacagaaaca gaccttttga aaaatctgaa tttgatcgac acctcaaaaa gcggtatgac
    29161 gctgacaact aagggcgaag aagtttatca aagccttgaa aacttcatgg atcagctttt
    29221 aggtacgcat caaaccgaac aacagttggc agaatatttt ggtaaccaac gctgtatcgt
    29281 tgtttcagga aacagcgaag agcagaccaa agtggctgat gcattcggcg aagcgctgag
    29341 cgaagcctta gaccgactac ttccagaagg cgagaatatc atcgctgtga tgggaggaac
    29401 tactatggct gtggtagccg aacaattgtc taatctggaa aataagaaac ggcataacct
    29461 gtttgtccct gcaagaggcg ggattggtga agcgataact gtacaggcaa attctgtcag
    29521 tgcaagaatg gctgctaaag caaacggtaa ccaccgcgca ctttatgttc cggaacaatt
    29581 aagtctggca acttacaatt ctttactcaa tgagccatct atccaagaag tcttaaactt
    29641 gattagtgaa gcgaattgtg ttattcatag tattgggcgt gcattacaca tggcagcacg
    29701 tcgtaagatg actgaaaaag aattagtcat gctgaaacaa gcaaatgctg ttgcggaatc
    29761 ttttggctac tttttcaatg aaaaaggtga agtcgtttat aaggtcccta gaatcggcat
    29821 agaattgcaa gatttggaaa aagtcccgat catcatggca atcgcgggcg gaaaatcaaa
    29881 agctaaagca attcgagctt atatgaaaaa tgcaccaaaa caaacgtggc tcatcactga
    29941 tgaagctgct gcaaatgaga ttttaaaagg ggtaaccctt taaaataaat atttttttga
    30001 ttttcataag gaggaaatct taaatgacag ttaaagtagg tattaatggt tttggacgta
    30061 ttggacgtct agcattccgt cgtatccaag atgtagatgg aatcgaagta gtagcaatca
    30121 acgacttgac agatgctaaa atgttggcac acttgttaaa atacgacaca actcaaggac
    30181 gtttcaacgg aacagttgaa gttcatgaag gttcattcaa cgtaaacgga aaagaagtta
    30241 aagtattagc taaccgcaac ccagaagaat taccatgggg cgaactaggc gtagatatcg
    30301 ttctagaatg tactggtttc ttcacttcta aatcagcagc tgaaaaacat ttaacagctg
    30361 gtgctaaacg cgttgttatc tctgctcctg gcggaaacga tgttccaaca atcgtttata
    30421 acacaaacca cgaaacatta actggtaaag aaacagttat ctcaggtgct tcatgtacta
    30481 ctaactgttt agctcctatg gctaaaacat tgaacgacaa atttggtgtt gttgaaggat
    30541 taatgacaac tatccacgct tacacaggtg accaaatgac tctagacgga cctcatccta
    30601 aaggtgactt ccgccgtgca cgcgctgctg ctgcaaacat cgttcctaac tcaacaggtg
    30661 ctgctaaagc tatcggttta gtaattcccg aattgaacgg taaattagac ggagctgctc
    30721 aacgtgttcc tgtaccaaca ggttcattaa cagaattagt aacagttctt gaaaaagaag
    30781 taactgttga cgaaatcaat gcagcaatga aagaagcttc aaacgaatct tacggataca
    30841 acacagacga aatcgtttcc tcagatatcg ttggtatgac ttacggttca ttatttgatg
    30901 ctacacaaac taaagtaatg acagttggcg acaaacaatt agttaaaact gttgcttggt
    30961 acgacaacga aatgtcatac actgcacaat tagtacgtac tttagaatac ttcgctaact
    31021 tataagatag acaaacgtac aagtgtaaat tatagacatg tacatttaaa agcggggaag
    31081 caatcgcgct tcttccgctt tttttgataa taagtttatt
//
LOCUS       DAMEET010000028        29110 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.028, whole genome shotgun sequence.
ACCESSION   DAMEET010000028 DAMEET010000000
VERSION     DAMEET010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 29110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.028"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1030)
                     /locus_tag="PB780_001108"
     CDS             complement(<1..1030)
                     /locus_tag="PB780_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDE0733173.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1112..1801)
                     /locus_tag="PB780_001109"
     CDS             complement(1112..1801)
                     /locus_tag="PB780_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDE0733174.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="PB780_001110"
     CDS             complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="PB780_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDE0733175.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2328..2831)
                     /locus_tag="PB780_001111"
     CDS             complement(2328..2831)
                     /locus_tag="PB780_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDE0733176.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3035..3162)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="PB780_001112"
     CDS             complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="PB780_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDE0733177.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="PB780_001113"
     CDS             complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="PB780_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDE0733178.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4799..5467
                     /gene="sdaAB"
                     /locus_tag="PB780_001114"
     CDS             4799..5467
                     /gene="sdaAB"
                     /locus_tag="PB780_001114"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HDE0733179.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5480..6352
                     /gene="sdaAA"
                     /locus_tag="PB780_001115"
     CDS             5480..6352
                     /gene="sdaAA"
                     /locus_tag="PB780_001115"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HDE0733180.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6443..7462)
                     /locus_tag="PB780_001116"
     CDS             complement(6443..7462)
                     /locus_tag="PB780_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDE0733181.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7635..8540)
                     /locus_tag="PB780_001117"
     CDS             complement(7635..8540)
                     /locus_tag="PB780_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDE0733182.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="PB780_001118"
     CDS             complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="PB780_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDE0733183.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9245..9415)
                     /gene="secE"
                     /locus_tag="PB780_001119"
     CDS             complement(9245..9415)
                     /gene="secE"
                     /locus_tag="PB780_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDE0733184.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="PB780_001120"
     CDS             complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="PB780_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDE0733185.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9753..10397
                     /locus_tag="PB780_001121"
     CDS             9753..10397
                     /locus_tag="PB780_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDE0733186.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10453..10956)
                     /locus_tag="PB780_001122"
     CDS             complement(10453..10956)
                     /locus_tag="PB780_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDE0733187.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10969..11015)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11553..12518
                     /locus_tag="PB780_001123"
     CDS             11553..12518
                     /locus_tag="PB780_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDE0733188.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12617..13789)
                     /locus_tag="PB780_001124"
     CDS             complement(12617..13789)
                     /locus_tag="PB780_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDE0733189.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13776..14921)
                     /locus_tag="PB780_001125"
     CDS             complement(13776..14921)
                     /locus_tag="PB780_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDE0733190.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14935..15867)
                     /locus_tag="PB780_001126"
     CDS             complement(14935..15867)
                     /locus_tag="PB780_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDE0733191.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15984..17063
                     /locus_tag="PB780_001127"
     CDS             15984..17063
                     /locus_tag="PB780_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDE0733192.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17060..17689
                     /locus_tag="PB780_001128"
     CDS             17060..17689
                     /locus_tag="PB780_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDE0733193.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17799..18026)
                     /locus_tag="PB780_001129"
     CDS             complement(17799..18026)
                     /locus_tag="PB780_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDE0733194.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18129..20717)
                     /locus_tag="PB780_001130"
     CDS             complement(18129..20717)
                     /locus_tag="PB780_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HDE0733195.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20737..21396)
                     /locus_tag="PB780_001131"
     CDS             complement(20737..21396)
                     /locus_tag="PB780_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDE0733196.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="PB780_001132"
     CDS             complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="PB780_001132"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDE0733197.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22169..23035)
                     /locus_tag="PB780_001133"
     CDS             complement(22169..23035)
                     /locus_tag="PB780_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDE0733198.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23144..23785
                     /locus_tag="PB780_001134"
     CDS             23144..23785
                     /locus_tag="PB780_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDE0733199.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="PB780_001135"
     CDS             complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="PB780_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDE0733200.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25226..26125)
                     /locus_tag="PB780_001136"
     CDS             complement(25226..26125)
                     /locus_tag="PB780_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDE0733201.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26127..26924)
                     /locus_tag="PB780_001137"
     CDS             complement(26127..26924)
                     /locus_tag="PB780_001137"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HDE0733202.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26902..27606)
                     /locus_tag="PB780_001138"
     CDS             complement(26902..27606)
                     /locus_tag="PB780_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDE0733203.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27737..28312
                     /locus_tag="PB780_001139"
     CDS             27737..28312
                     /locus_tag="PB780_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HDE0733204.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28430..29107
                     /locus_tag="PB780_001140"
     CDS             28430..29107
                     /locus_tag="PB780_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDE0733205.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8566 a         6169 c         5232 g         9143 t
ORIGIN      
        1 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
       61 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
      121 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
      181 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
      241 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
      301 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
      361 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
      421 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
      481 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
      541 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
      601 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
      661 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
      721 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgcta
      781 ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact aaaaacagta
      841 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
      901 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
      961 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt gttttttcat
     1021 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
     1081 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
     1141 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
     1201 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
     1261 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
     1321 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
     1381 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
     1441 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
     1501 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
     1561 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
     1621 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
     1681 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
     1741 ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca
     1801 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
     1861 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
     1921 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
     1981 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
     2041 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
     2101 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
     2161 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
     2221 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
     2281 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
     2341 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
     2401 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
     2461 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
     2521 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
     2581 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
     2641 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
     2701 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
     2761 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
     2821 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
     2881 aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac ctattcactt
     2941 cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt tgctcaacca
     3001 gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca
     3061 ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg
     3121 cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg
     3181 ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa
     3241 ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt
     3301 tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc
     3361 tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat
     3421 atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt
     3481 tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa
     3541 tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa
     3601 gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc
     3661 agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt
     3721 accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc
     3781 tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt
     3841 tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc
     3901 atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg
     3961 gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct
     4021 tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc
     4081 gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc
     4141 ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt
     4201 cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga
     4261 atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg
     4321 tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag
     4381 gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt
     4441 ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc
     4501 atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac
     4561 aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt
     4621 tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct
     4681 catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata
     4741 acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat
     4801 gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag
     4861 tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga
     4921 acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca
     4981 cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt
     5041 agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag
     5101 tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct
     5161 ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt
     5221 caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg
     5281 tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac
     5341 agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca
     5401 agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt
     5461 cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc
     5521 ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg
     5581 tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga
     5641 gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga
     5701 tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa
     5761 tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc
     5821 cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac
     5881 acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa
     5941 caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc
     6001 aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc
     6061 agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt
     6121 tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga
     6181 tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta
     6241 ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt
     6301 tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc
     6361 cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa
     6421 ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact tcatcgatcg
     6481 tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag
     6541 atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg ccaatcgcaa
     6601 ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg acaccagagt
     6661 cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat acttcaaagg
     6721 acgcaggacc tccatctaat tgacgtccac catgattgga tacccagata ccatctgccc
     6781 ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg ataactggaa
     6841 gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt tttgcagctg
     6901 cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt tcgctatacg
     6961 cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct tcttcacggt
     7021 aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct tctactgctt
     7081 tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg aaaaactgcg
     7141 gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg
     7201 aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct ttttcatgtg
     7261 ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca attccgaaaa
     7321 cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg attttctttg
     7381 tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca ccgcgaatat
     7441 agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag gcaatgttta
     7501 caatatcgat tggatgttct tcattacttg tatgataagt catagtagat ccttctttca
     7561 tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac atttactctc
     7621 agatactttt atcgctattc tttccaaata aatcgactta caagctgatc tacttcttgg
     7681 ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc ctctatttca
     7741 tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg tactgttcca
     7801 tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt ggttatagga
     7861 atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc aggccccttt
     7921 gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca
     7981 attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt tcctaaatag
     8041 cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc tatgccgtag
     8101 ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca ctcgttattt
     8161 ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga caaatctcct
     8221 gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac tgcttgctga
     8281 tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct gtagccgtgg
     8341 aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc cgttttttct
     8401 ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa
     8461 ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat tggttctgtc
     8521 atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat catatgatcg
     8581 acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa gttctgtact
     8641 ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc cgtcaacttc
     8701 tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga cttcgccaac
     8761 ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga tatgattgac
     8821 ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt
     8881 cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca tttcaacaag
     8941 tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt tgacttcagt
     9001 ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca tactttgcgc
     9061 acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat gcaagacata
     9121 ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc
     9181 tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg ttgtggctag
     9241 cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt acgtaaaaca
     9301 tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg agttgttttc
     9361 tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat ttcatgagac
     9421 aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat tacaatattt
     9481 acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg attttgtgta
     9541 gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca tttacttctg
     9601 cccccttgct gagaatcaga cttatacttc ataacagtat cacgtaactt tttacttgtc
     9661 aatttgtgaa ataaactatc tattgttttg gataaaatta tgatatgatt aatttgaaat
     9721 taagctaaaa tgagacaatt ggaggtatag cgatgctatt aaaaacactt gtatataaga
     9781 aaaaagatct gacaaccgtt acagagacta gtacattaga agaagcatta aaaattttag
     9841 aaacttccgg ataccgttgt gtgccgatct tagattcttc aggtaatatt ttccgtggaa
     9901 acatttacaa aatgcatatc tatcgtcata aagcaaatgg cggtgatatg acgcttcctg
     9961 tcactcatct tttgaaaaac gctacaaagt tcatttacct ggatacttct ttcttcaaag
    10021 tattctttac gatcaaagag cttccttata ttgctgtcct tgacagtgaa aatcattttt
    10081 atggtatttt gacacatagt acgttgctta atatgctctc tcaatcttgg agcgtagaac
    10141 aaggaagcta tgtactgaca attgcttcca caggaaagca aggagatttg gctgctattg
    10201 caaaaatcat ttcaaaacac agcagcattg ccagctgtat tactcttgat atcgaaaaag
    10261 acgaatacat tcgacgtaca ttgatcacgc taccggctaa cactgaagcg aaagtatgtg
    10321 aaacgatcgt caaccactta gagaaaaaga attataaagt ggtagaaatt gaaaatttgc
    10381 aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt
    10441 cgctttttat aattagacac gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa
    10501 tggtgctgaa acagccatga cgataaactc acttaatgca agcgtgccat aggttgtcca
    10561 aaatgcttgg ccacctgttg gaactaacat ataagcaatc agtcccatgc tgatggtaaa
    10621 gaagatcgtg ttcaatacta aacgcatctt tacatttttc actttgtttt gcaataaagc
    10681 tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa accacatcca tgataccaaa
    10741 accaaagaag gcattataga cgatgacccc gcctaaaact ccccaaagca atttacggtt
    10801 aaagacaact aaatgattca aactttctga aatacgaaac tggatcggcc ctgaggatac
    10861 aggcgcaact aaaatagtta acgctagata aagagccatg atgatgccat ttacaacgat
    10921 tacggataac cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt tctcctagtt
    10981 tttttaacgc gggatggttg atgaaccgcg gttatgtgaa acaaacaata attataacag
    11041 acttttcatt tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg
    11101 taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg tcataagaat
    11161 tcccagtaaa gcagacgaag taggatctgc aagaacagaa ttgttgtgaa acttaaataa
    11221 ataaaaaagg atgtgggaat ttgagcaacc tcttactaga gaacggtcta gcaagcagct
    11281 gaagatctaa gtggacatgc cgaaccttgg aacttctcct tacgtaagta atggatcatg
    11341 cgcttgctag gcttttttgt gtttttcggt cttcggacct ttttcatttt cctagacttc
    11401 tgctataata accaagaatt aattttgtaa cagatttttt tgaaaaaaca agtttctttc
    11461 attcaaaaaa tagaaaaagc ttgctaatta ttggttaacc agcaaacagg agtattaaaa
    11521 tatcttgttt tattgaatag caggaggaaa tcatgaaatt ccattatcat atattgatca
    11581 atccatcggc aggcagcgga aatggctata aagtagcaga aagaatttta ccggtattaa
    11641 aaaataagca tattgactat acacttcatt atagtgaata caaaggacac gatgctgaga
    11701 ttgctgaaac tttggcaaaa gaaacattga ttccttggat agaagaagaa cagaaaacaa
    11761 tcgatacttt cccccttttg atcatcgtag gaggtgacgg caccttacat caggtacttg
    11821 atactttcta tcagatggaa gtagaattcc cagttgctta tattccagca ggttctggaa
    11881 atgattttgc tcgaggggca ggccttccaa aaaatcccaa aaaaggactt cagctgattt
    11941 tagcagctca atcaccagaa aaagtccaca taatggctta tgaagaaaaa ataagtgaaa
    12001 aaaaaggaat agctgttaat aatttcggta taggattaga tgcagctatc gtccatgcga
    12061 caaatcattc ttccacaaaa aagcgattga acaaatacaa cttaggatca ttttcgtatt
    12121 tattctctat tctccgtgct ttattcactc aaaaaggatt tccaattctg gtagatgcag
    12181 gaggaaaaca attaagtttt tcaaacgcat ttttatgtac agcaacgaag catccttttt
    12241 ttggcggagg aatcgcgatt gttccaactg ctgatgcgtc taaaccagtc attgattttg
    12301 tcgtggtcga acgaatcaat ttctttaaaa tcttgtggct cattctttta cttatacaga
    12361 aaaaacaatt gaaatcgaaa tatttccatc actacacaac aaacagatta cgtatcgttt
    12421 caacaacacc gcaacatggt caagaagatg gagaagaaat ggaaaaacga ccttttgata
    12481 tcacaataac cacaactaaa cagttgtttt ggtgttaata aaagaggttt tgtcagaagc
    12541 tacatctgga acaccgttta ttcggaaaaa aggactgtga cacgactttt gtcacagtcc
    12601 tttttttact atgcagttaa ttctttgccc aattgggttg ccatcgatag tttctttctt
    12661 tacccaaaat aaattggatc attgaaaaca ccgcagcaaa agcaactaag acacaaccat
    12721 tgaaccaaaa agattctgca aatgtttgat tgtaactgag tgtttcactg atcaagttat
    12781 tattttgaac aaaccaatat gttggtgtga atgccgctat tttgctaacc atttctggca
    12841 agtattctgc tggtacaaac accccaccga taaagcaact gcctgtgata aaaatatctt
    12901 tgatcccacc gctggtttct gagtttttta ccaatgaagt gatacaagcc gaaaagctaa
    12961 tgatcggcaa taaaaacaag aaagtattta aaatatgata ccataccgtt atatcccacg
    13021 aggtgtggat cactaacaga accattagaa gtaagaagcc actgaacaac agcagcgagt
    13081 aactaatcaa actaaaggtt actttgcgat tcaagtttcg tttggtaatg ggcgcactct
    13141 ggtttcgttt actgatctct ttgcgattaa acgctagatt gatgacgctg aaaccgctaa
    13201 aaatggatag aaacaaccca taagacaaga aattaaaggt tgtgcccgtc aacagctttt
    13261 tcatccgctg actatatccc gcacttagct caacttctcc agtaaccgag agtgccattt
    13321 gtgtctgttc catagctgca tcgattgttt tatcttcttc ttgaaacaga atcagtgttt
    13381 gcaaaaaggt gttcacttgc tgtgtaacaa ctgttttaga aaaggcatct gggcgactct
    13441 gtatcttgat cgtcgccatc tttcctactt cgacttgttc ggtaaagtct gcaggcagat
    13501 gtaagataat gtttacacga ttaaaataaa gagcatcatc gatttctttt tgtgaggttc
    13561 catccaatgt aacgactgtt tccttgtcat tcaaatattt acgaaggcca tcgactacag
    13621 gcgactcttc ttcactaatc aaggcgattt tcgcatcttg cagagaggta ttttcaacat
    13681 caatattttg cgcaaaaaag aatgtggcaa caacagtgat aattacacct atcagtaaat
    13741 tcgatttatt tgctttaagc aatgtccaaa tcgttttata aagcgtcata acgcacccct
    13801 ctttccatcc aagcatttcc taacgcaaaa aggaaaataa atccgatcaa ccatagaaga
    13861 ttggtataaa aactagccaa atcagtgtag aaataaagtt tatacaaact ttcgctgatc
    13921 aagttgacga gatttaattg accaacaatc ggaatatgaa gatcaatcca atacttcact
    13981 tgttccgacc ccatcatacc agcaaaaaag ctcatcgcaa tcgtcactga aatcaagatg
    14041 ctatcctttt gcaccagatt catttttggt atcaaattac caaccaatgt acctaacaaa
    14101 atcgcacaca acgaccccag agcggtaaca atcatcaacc attgccaacg ttcaccaaag
    14161 tctacttgat agataaaacg aaagacagct aacataatat aaatctctgt caaaacaatc
    14221 gtaaagcttg ctgccaaatt agcgattgaa actaacgatt gctttctagg aatcaaggac
    14281 aagcgaatac catttgacga ttgatttgct tgctgatcct gtacattgcg gaccccccac
    14341 atcgtcccgt agagaatcgc cattccaatc agagtgaaga aataaaaact tttgaaactc
    14401 atattacgac ctgcatgaac ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg
    14461 ggcgcgagat caacgttctc cacttctgcc actttactcg aaattatacc aatacgctgt
    14521 aaaaattgat ccatgacgtt tttcatcaac gtctgactca ttcctgcttg acccacatgg
    14581 agcgaaacag atgaaccatc aatgagataa aaaccagcca aatcatctgc atttagcttt
    14641 tcattcgctt ctacttccga tagttcctgt acttcaaata atggtttatc attgacttcg
    14701 acttgttcta agactgtggt aaaattttcc gaaagcgctg tttgatccgc tttgattaca
    14761 attccagtat taatcgtttc aagattgcca ccttgatcaa tctcgccaaa ggccgccata
    14821 aacatcaatc ctaaaagaat tggaaaggcc aatgtccaaa acaacatgga tttatttcgc
    14881 agcagtattt ttgtgcgata tttataaaaa tgccaaaaca ttatttcccc tcctctagtc
    14941 tctcaaatcc ttgccagtaa tctctaaaaa gacatcattc aaggacgctt ctttggttgc
    15001 tatttttccg taactgatct tttcttcttg taagattgta agaatcggca acaatactgt
    15061 atttagttca gtagttgtta cttggagctg acctttttta taagcgattc catcagaagc
    15121 tattgttcgc tgaatcgctg acaacacatt ttctgggagt aatgggatgt cgatcgtcaa
    15181 acgatccgcc gtccgtacca tatcttttag agattctttg gttcctttag caatcacctg
    15241 tccttgatcc atgatcacga tctggtcgca aagaatttct acttcttcca tgtaatgtgt
    15301 tgtatagaca atggttgctc cttgtcgatt cagctccttg atactttcta aaattttatt
    15361 ccgactttgg gggtctacag ctactgtcgg ctcatctaaa aaaattaacc gcggcttgtg
    15421 ggcaatccca catgcgatgt tcaaccgacg tttcaagcca ccactaagtt ctcctggacg
    15481 aaacgaaaca tatttttcta atcccactaa ttgaatcacc tcttcaatca aggctttcct
    15541 tttctctttt tcggaaacat ataaaccgca aaaataatcg atattttctt gcacacttag
    15601 ttcgtcaaag accgcaacgt cttgaggaac gaccccaatc attgccttga cagcataatt
    15661 ttcaggagtc attgttgtcc cgaacaattt aatggttcct ttgtcataat ttaacaaaga
    15721 aagcatacag ttgatggcag tcgatttccc actgccgttt ggacctaaca gacctaagac
    15781 ttctccttct tgtacttgca gatcaaaatg attcaatgct acaaaatctc catagcgttt
    15841 gactaattta tcaatttcga taatcatcat gaaaaactcc ttccaaattt cttgttgatc
    15901 tgactataat aaagtgcaag aaaagaaact agtgacgatt ggctaaacca cacatgacat
    15961 ttgtcacaat gaaggagtct gctatgcata aatctcggct actcaacgaa actagctttc
    16021 tactggttat ctcattgctt ttgctggtaa gtcaaggatt cgttgctatg caagttatct
    16081 atttattgat cgccgtttta ttcactggtg tcagttattt actgcctaca aagccaaatt
    16141 ggattttcca agttattcta tttcttttat gtgccttttt acctgatctt gtcctgtttc
    16201 tgccagcgac gatgcgagtg atcgcccctg aagaaagcca ccttgattac ttgtttttgt
    16261 taggtttttt gttgatttcg acggttggta atcatccccc catcaaggtc gcctttgtcg
    16321 taattttgct gtttttttcc ttttacttac gaaaaaaaga tcaacgaacg accgatcttg
    16381 agaaaaagta cattgtctcc aaagatactc attgggaaaa acaacaagag ttatcggaac
    16441 aaaataaagc attggccctg tcacaagaaa cacaattagc cttgcaaatt gtggaagaac
    16501 ggaaccgaat tgcccgagac atccatgaca atgtcggtca tctgctgtca agtgcgattt
    16561 tacaaattgg tgcgcttgaa atgatcaatc aagaaccaaa actaaaagaa ccattagaaa
    16621 atttaagtgc caccgttcac actgggatgg atcggattcg tcaaagtgtc catgacctcc
    16681 atgagacatc gatctctttt caacactcct tagagtttct gatcgctgat tttccttctt
    16741 ctgttacgat tgaaggaaac ttgtttgaag gattgaatga aacgcagcaa aactgcttct
    16801 taatggtcat caaagaagcc ttaaccaaca gtatcaaaca tagtcatgcc aaaaccgttg
    16861 ttttacattt tcgaacgttg ccggcctttt atcgcttgca aatcacgaat gatggcacca
    16921 gcccacaaaa aaattctgct cccggtattg gtttgaaaac gatgcgtcaa cgtgttcaag
    16981 aacaaaaggg acaagtccat attttccaag aaaaccagca atttcagtta acgatcattt
    17041 taccaaggga gggacaccaa tgataacagt agtcattatt gatgatgatc catttgtgac
    17101 gaaatcgtta caaaccattt tagaaagcac gaaagagatc cgtgtgttag gcattggtca
    17161 ttgtgccaag gatgcgctag tcttatacga aacccatcgt cccgatgttt tgctgacgga
    17221 catccgcatg cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc
    17281 caaagccatc cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt
    17341 ttccatcggt gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt
    17401 caaatccgct tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca
    17461 aatgatgaag aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga
    17521 gcttgcaatc atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt
    17581 gtacttgagt gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt
    17641 acgggatcgc acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa
    17701 cacaaaaaaa cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt
    17761 caattgtccc gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca
    17821 ggattcgttg gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt
    17881 tctagttttg attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat
    17941 tctttttcca tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct
    18001 aatttatctt cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca
    18061 tatcacactg aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat
    18121 ctttaaaatc aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca
    18181 atcatcggat ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa
    18241 acagcaggtt ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt
    18301 tccactgttt ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt
    18361 agcttttctt ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt
    18421 ccgaccaaag aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca
    18481 ttatccatga tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa
    18541 gtgacatagg cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc
    18601 gtgtagagca aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc
    18661 atcttgaatt cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta
    18721 ttccattcgt ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc
    18781 actttatctt cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg
    18841 aatacattca attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg
    18901 gtgtcgttcc aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc
    18961 attttattta acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata
    19021 tcttgtgcac ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca
    19081 atttgataag catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt
    19141 ggcagacgtc cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc
    19201 cgataaatct gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt
    19261 acttgaccag gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata
    19321 ttcgtcggaa tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca
    19381 tcaacaatca aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt
    19441 ccattcagcc ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt
    19501 cgtttagctg ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat
    19561 tttgaaggat ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc
    19621 acagtagttt tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct
    19681 tcagcgatag cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc
    19741 cgaatattct tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg
    19801 cgctggatcg aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta
    19861 gtctcttctt gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga
    19921 tcaggctgga tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat
    19981 gtctccgccg gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag
    20041 atagctgctc gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta
    20101 tctgcacgtt tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg
    20161 atgatatcca aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg
    20221 aacccgtaac gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc
    20281 tcagcaagca tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga
    20341 tcagcaatga ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt
    20401 ttcttcccga ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt
    20461 gttggctttt cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc
    20521 actaattctc cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg
    20581 actacaatct ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact
    20641 ttgtaaaaat tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc
    20701 atttcttctg gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt
    20761 ttcagttcct aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca
    20821 aggctcttca gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa
    20881 ctcacttagc tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc
    20941 tacgaataaa agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat
    21001 acgtgtgatt gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa
    21061 atgattacgc aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg
    21121 accttctaaa gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg
    21181 acgagcaagc tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga
    21241 ataaattgct ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta
    21301 actttgtggc aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca
    21361 ttgtgtcttc ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt
    21421 ttattttact ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa
    21481 ttctaaatta gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt
    21541 cgataaattc aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc
    21601 gttttcacgc ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg
    21661 ctcttctcca tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa
    21721 atgtagttta cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat
    21781 atttacatca ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa
    21841 acctagtggc tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt
    21901 tccagtgttt gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc
    21961 tctcatattt atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt
    22021 atcaactcgg tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt
    22081 tatcagaaca ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc
    22141 ctcaactttt ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag
    22201 ttccttttaa taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg
    22261 ctttgattcg ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat
    22321 tttcctctgg tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt
    22381 cagcaaactt ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat
    22441 aaaaagcctg acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc
    22501 catcttctga gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa
    22561 ccgcgttgaa tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg
    22621 cttcggcaga ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag
    22681 cattttgaat ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa
    22741 catctaatag cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat
    22801 acatgccaga ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt
    22861 taaccgcatg ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat
    22921 ttttgcatat caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga
    22981 acagctgatg attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc
    23041 cccctcattt ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg
    23101 atacaatgcc tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg
    23161 cgctgaaaac tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt
    23221 gtgtgacaac ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt
    23281 aagttatgct ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga
    23341 cttcgtctat aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa
    23401 attagggacg gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga
    23461 ggccttagaa ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt
    23521 tgacagtatt cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt
    23581 tgaccgtatc ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg
    23641 gcttaaagaa ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt
    23701 cactgcagag aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac
    23761 caaaatcgtg gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa
    23821 tgcttattgc aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata
    23881 ttgaaataaa tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct
    23941 actgctggat caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc
    24001 gccactgtga ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt
    24061 ttccaaaaac ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg
    24121 gtcaagactt cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca
    24181 agtcgacgaa cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc
    24241 agcgaaatga agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt
    24301 aaagaagcgg tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac
    24361 gctgccttta ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa
    24421 tcgctagcag cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac
    24481 aaatgatcat cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt
    24541 gccacatctt cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca
    24601 gaaatcattg cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc
    24661 tgaccttctt gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc
    24721 ttcagcacgt acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt
    24781 gtcatgatcg cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca
    24841 tcgtctgccg acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc
    24901 aagtcaacag cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc
    24961 atttcatcta ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc
    25021 gctaattctt taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat
    25081 tggtagaatt gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga
    25141 atattatttt cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt
    25201 tcatccaaac caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt
    25261 ctgcggctaa aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca
    25321 gatcataaca atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat
    25381 catcaccgag cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc
    25441 ctgtatgcag ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact
    25501 cagtctgtgc cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc
    25561 gggcgatcgg caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta
    25621 aggcctgata ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg
    25681 caaatccatg ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca
    25741 catggattac ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca
    25801 ttgtcccatt tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta
    25861 ccagaacgtg ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt
    25921 catgttctcc ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca
    25981 agacattttc tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta
    26041 acagaccctt tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt
    26101 tctcataatc ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga
    26161 tgccaaaaat gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa
    26221 taaattgagc ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct
    26281 tctgtatttt ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc
    26341 aaagtacgaa acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata
    26401 ctaggatgga tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta
    26461 gaaatcgtca atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg
    26521 gctaccatcg tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg
    26581 tccggtgttt catccaaata actgatccga acatccagca agggataact aacactttgt
    26641 tcccggttcg ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg
    26701 taaaacccta aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg
    26761 tagtgatgga acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc
    26821 tcatgatcat ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact
    26881 tctatagtct ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct
    26941 ttcgctttct ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg
    27001 ggagaataac cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa
    27061 ctgataggca gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc
    27121 ttgatattta taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg
    27181 aatctggatt tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat
    27241 caccagatga taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg
    27301 gagaactttc atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac
    27361 aaactgacac atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat
    27421 tggaatatgc cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt
    27481 gacaaattca atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag
    27541 tttcactttc aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg
    27601 ttccatagct gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa
    27661 gttttttcgt actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca
    27721 aaaaggagtc gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa
    27781 gcaagagtac gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca
    27841 gactaatatt tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg
    27901 gatccgtttc gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg
    27961 tctcgaaaca acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg
    28021 tctgcctcgt tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc
    28081 attaatccaa accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact
    28141 tttagctatt gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt
    28201 aggggatgca tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc
    28261 ttatcgtcct gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt
    28321 cattttcaca aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg
    28381 aaagaatcta gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat
    28441 ctatttattt gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca
    28501 atttatcgaa gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc
    28561 tttggtcaat ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa
    28621 tcttgaggaa cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc
    28681 ggcgcagttt caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt
    28741 cggctgtaat gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg
    28801 cggcgatttg aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt
    28861 ttctgatcaa aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta
    28921 caattatgcc tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct
    28981 ttggagaatc acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga
    29041 acatttacat acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc
    29101 taattaaaaa
//
LOCUS       DAMEET010000029        28577 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.029, whole genome shotgun sequence.
ACCESSION   DAMEET010000029 DAMEET010000000
VERSION     DAMEET010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 28577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.029"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(290..1087)
                     /locus_tag="PB780_001141"
     CDS             complement(290..1087)
                     /locus_tag="PB780_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDE0733206.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1084..2268)
                     /locus_tag="PB780_001142"
     CDS             complement(1084..2268)
                     /locus_tag="PB780_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDE0733207.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2293..3540)
                     /locus_tag="PB780_001143"
     CDS             complement(2293..3540)
                     /locus_tag="PB780_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDE0733208.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3755..5500
                     /gene="dnaX"
                     /locus_tag="PB780_001144"
     CDS             3755..5500
                     /gene="dnaX"
                     /locus_tag="PB780_001144"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDE0733209.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5520..5834
                     /locus_tag="PB780_001145"
     CDS             5520..5834
                     /locus_tag="PB780_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDE0733210.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5859..6455
                     /gene="recR"
                     /locus_tag="PB780_001146"
     CDS             5859..6455
                     /gene="recR"
                     /locus_tag="PB780_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDE0733211.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6519..7163
                     /locus_tag="PB780_001147"
     CDS             6519..7163
                     /locus_tag="PB780_001147"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDE0733212.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7178..7507
                     /locus_tag="PB780_001148"
     CDS             7178..7507
                     /locus_tag="PB780_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HDE0733213.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7521..8459
                     /gene="holB"
                     /locus_tag="PB780_001149"
     CDS             7521..8459
                     /gene="holB"
                     /locus_tag="PB780_001149"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDE0733214.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8495..9319
                     /locus_tag="PB780_001150"
     CDS             8495..9319
                     /locus_tag="PB780_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HDE0733215.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9312..9659
                     /locus_tag="PB780_001151"
     CDS             9312..9659
                     /locus_tag="PB780_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HDE0733216.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9728..10600
                     /gene="rsmI"
                     /locus_tag="PB780_001152"
     CDS             9728..10600
                     /gene="rsmI"
                     /locus_tag="PB780_001152"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDE0733217.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10709..11830
                     /gene="dinB"
                     /locus_tag="PB780_001153"
     CDS             10709..11830
                     /gene="dinB"
                     /locus_tag="PB780_001153"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDE0733218.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11884..12486)
                     /gene="nrdG"
                     /locus_tag="PB780_001154"
     CDS             complement(11884..12486)
                     /gene="nrdG"
                     /locus_tag="PB780_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDE0733219.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12801..14990)
                     /gene="nrdD"
                     /locus_tag="PB780_001155"
     CDS             complement(12801..14990)
                     /gene="nrdD"
                     /locus_tag="PB780_001155"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDE0733220.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15289..16083
                     /locus_tag="PB780_001156"
     CDS             15289..16083
                     /locus_tag="PB780_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDE0733221.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16073..18115
                     /locus_tag="PB780_001157"
     CDS             16073..18115
                     /locus_tag="PB780_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDE0733222.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18396..18545
                     /locus_tag="PB780_001158"
     CDS             18396..18545
                     /locus_tag="PB780_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HDE0733223.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18556..20070
                     /gene="dltA"
                     /locus_tag="PB780_001159"
     CDS             18556..20070
                     /gene="dltA"
                     /locus_tag="PB780_001159"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HDE0733224.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            20067..21272
                     /gene="dltB"
                     /locus_tag="PB780_001160"
     CDS             20067..21272
                     /gene="dltB"
                     /locus_tag="PB780_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HDE0733225.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21317..21550
                     /gene="dltC"
                     /locus_tag="PB780_001161"
     CDS             21317..21550
                     /gene="dltC"
                     /locus_tag="PB780_001161"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HDE0733226.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            21553..22821
                     /gene="dltD"
                     /locus_tag="PB780_001162"
     CDS             21553..22821
                     /gene="dltD"
                     /locus_tag="PB780_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HDE0733227.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            22962..24044
                     /locus_tag="PB780_001163"
     CDS             22962..24044
                     /locus_tag="PB780_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDE0733228.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            24044..24847
                     /locus_tag="PB780_001164"
     CDS             24044..24847
                     /locus_tag="PB780_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDE0733229.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            24917..25657
                     /locus_tag="PB780_001165"
     CDS             24917..25657
                     /locus_tag="PB780_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDE0733230.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            25668..26672
                     /locus_tag="PB780_001166"
     CDS             25668..26672
                     /locus_tag="PB780_001166"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDE0733231.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(26717..28561)
                     /locus_tag="PB780_001167"
     CDS             complement(26717..28561)
                     /locus_tag="PB780_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733232.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         9011 a         5052 c         5878 g         8636 t
ORIGIN      
        1 ctctgagtca caaggaacaa tttcaaaaaa cagcttttca tgtttgatga atattgtgac
       61 ttgtgaccga ggagttggta ggagaacacc gtttaccaag aggttgtgaa aaagctgttc
      121 tgcatcaagt aataagaaga gccaagtaaa aacagtctct tatgttttta acttggctgg
      181 acttatcacc gtagatgtaa gcttttgaac gccgtttatt tgcaacaaaa ggggctgtga
      241 cacgattttt gtcacagccc tttttttgcg aacattcatt tagcaatttt tagaaacttc
      301 tgccgccacc gccaaaagtc ccgccaccgc tagaatgggt agtacttccg ccaccgccgc
      361 ttccaggagg tgggcttttg gggattcttc ttgtcgtgac aaaagaattt gtcagacgat
      421 cggttttttc agttaatttg atactggatt tttctcggaa tggatatttg tagatgcctg
      481 atttcaattg ataacgcgaa acagtcacaa aataaaagac aagactaagg ataagagcca
      541 aaacaacagc aatgatgatt tctactgtcg tcaaaacctt atagcgtgta attttgccag
      601 tttcttcgtc tacacgataa tgtcctcctg gaactccgtc ttccacatat gttgctgatt
      661 tagacaggta agcttttgca gcattgtaat aggcagcatt tgtcatctga tcataaacat
      721 catctaagat cgaatcgatt ctgctatcag tcaaataatc gatcatatta ccagaagtcg
      781 aaatgtagat ttcacgctgt cccatatcta atagcaatac tgagccattt tgatcatttc
      841 cgactgcgtc tcttaagtaa ttatcagcaa agtctcttgg ttcttctgaa ttagatgtag
      901 tcgttacgat caagactcgt cctttgattt tttcattcag cttttctgcc tgttgttcca
      961 aagattggat ttgatctgtt gtgaataacc cagcctcatc atttaccgta gctgtatctg
     1021 catgagtagc caccggccag ccaacactta ctagcaaaag aaagaataaa aaaaattttt
     1081 tcatcatatc aagtaacctc cgatcagaaa gaggatcgct agcccagcga aaatcccaag
     1141 agcggtcagt cccaattttt tctggctgat cggcaggaca ccgctgacct ttcctgtttg
     1201 tccattcatt gcgtagtagt agactttttt acttgagtca ttgcttcgat aagtaacaag
     1261 ccataccggc agtaatacgt aattatttac ttcgtttgtg attgaagcag aagttcgcac
     1321 accggtcaat gttgtatatc catttgcggt atcttttaac agcttctctg aatagtcttt
     1381 taattcagat tgaatctgtg atttgattgc ctcatattcg atatctcttt tttctgcttg
     1441 gaacccggct aaatactggc ttttaaaatc aattgcttta tctaacggga aaggttgcac
     1501 gccttcgacc atcttttgtt gcgtattttt tgataaggca ttttttacta gttctttaaa
     1561 agaaagcttg cctgctcttg aaacagcaaa ttgcttcgtt tctgtgtatt cgatgtcacc
     1621 tatccgccat atgcggataa ctgtaccatt cgcctgcatg gcaccatcta cttcagcatc
     1681 tgtagcccaa taagggaaat acacacctgt caacttatcg atttgatcct tattgaaaaa
     1741 agctttggga acaaaccatt ttttcttggt ccaagcgaga aatctgttga ttgcttcttc
     1801 tttctcgaca gcaaaaggaa gtactttatt tggcaagaat tctccgctta aacgtcccga
     1861 tagtacgacg ggattatggc agtagtaaca ataagtcgca gcagttgtcg catcagtcac
     1921 gatctctgca ccacagttgg gacaaagaaa aagatctatc gctgcggtat tatctgcaga
     1981 ttcgtccgct gtatctgaaa aaccgcctgc ctcgtcaacc gctttgcgct cggcttcatt
     2041 catatcggca agctgttctt gggctgtaaa agtaaaatct tcggtaggtg aaggttcttc
     2101 tttgctttct tcgccagcca tacgcgcttc tttttgcttt tgctcatatt ggctgacttc
     2161 ttcttctgta tagatattca ggcagtattc gcaatgaaat ttttggtctt tgggatcaaa
     2221 agtcaacgga ccgccgcaat taggacattt atgtgtaaga acatccatca gctgcacacc
     2281 tttctaaata aattaattaa cggggattcc tttaaacggt ttaccacagt ttggacagaa
     2341 tttagggatg ccgttggaaa gatccacgat ctcattgcat tcactgcatc gcatttgtag
     2401 cttaggctga acgttttcta caggtttggc agctccgcag ttggaacaga attttccgga
     2461 attttctgtc ccgcaaactg gacatgtcca agtatccgca tttccttgtg cagtttgttg
     2521 attttgtttt tctgcttgtt gtttgatttg ttcttggtta ttttgagctg cttgagaaag
     2581 ataagaacca tttgcattca ttcccatacc tacacccata aagccggtca ttgctcctgc
     2641 ttcatttttt ccggctgctt ccataccacg tgcaatggaa ccttgcacgt atccttcacg
     2701 tacgctagga tcacctagca tcgccccttc gttacgcata ttgatcagtt tcacagaatc
     2761 atcggtgtaa gaaatgctgg ctacagccac agaaacaatc tccatcccac gtaattcacg
     2821 ccaagaatca tccaatgcag tatccatgta tttgcttaat tccaaactct tagatggtac
     2881 ataagaaatc cgttgaccat ctgcagacat ctgattgatt gctgattgta gcgctgttag
     2941 gaattcagct aaatactgct cattgatatc attgatttcc acttgtgttt tgtttttagg
     3001 gattgcgttt gtataaaata aaatcgggtc aacgacatga atagaataag tgccatgtgc
     3061 ccgtaagaat aattctgcgt tatagaaatt gtcaaagtag ttcaacggcg cgcttgtacc
     3121 gaatttgatt ccctttattt cttggagatt gatataaaaa acttgttgtt tctgcggtgt
     3181 gacgccgcca aatttgaatc ggtcaaaagt ttcagcaatt gcttctttca gtgttccatt
     3241 gaacatggaa ggagctgcgt catttttaat ggtgtaataa ccttcctcag cggtataatc
     3301 gatgattttt ccaccatcga ccaaaagcat catcatgttt ggatagacat ggacgactgt
     3361 tccgtctgtt aaaacatctt ctgttccttt acgattggag ccacgtttgt catctttgcg
     3421 gactttgaca ccttttgtca tgaccgttgt atcactcata ttgtctggtt cgatgacttc
     3481 tagccattga tctgccagac cgccgccaac catacttgtt gctgctttga taagacccat
     3541 aaaaaattcc tcctctgatt cagctagtgc caaacaatta ttgtttattt attataccat
     3601 aggaagatgt gaaacttctc ggactagaga cggaattgaa atggaaaatt gcgtattgtt
     3661 taaatacctg aaaaagccga gaaggatagt agttttttta cctaatatca cgtataataa
     3721 gagatagtga attttttaaa aaagggggat ccttatggca tatcaagcac tttatcgagt
     3781 ctggcgttcg cagcgtttcg aagacatcgt tggacaaaaa gctgtgacac agacactaaa
     3841 aaatgctatt gtttcccaca aaacctcaca tgcctacttg tttacaggac ccagaggaac
     3901 cggaaagacg agtgcagcaa agattttcgc aaaagcaatc aactgtccga acagtaaaga
     3961 cggtgagcca tgcaacgaat gcgaaatgtg tcgatcaatt actgctggta cgcaagaaga
     4021 tgtgatcgaa atcgatgctg ccagcaataa cggagtcgaa gaaatccgat ttatccgtga
     4081 tcgggcaaat tatgcgccta caaaggcagc gtacaaagtg tacattattg atgaggtaca
     4141 tatgctttca acaggagctt tcaatgcctt gttgaaaaca ttggaagagc caaaagaaag
     4201 tgtcatattc atcttagcaa cgaccgaacc tcacaaaata ccagctacga tcatttcacg
     4261 gactcagcga tttgatttta aacggatcaa tacggcagat attgtcgaac atcttgaatt
     4321 tatcttgaaa gaatcgacta tcagctatga accagctgca ttgaatgtga ttgcccgttc
     4381 tgcagaaggc gggatgcggg atgcattgag tattcttgat caggcaatct cgtttagtga
     4441 aggaacaatt actttagatg atgccatgca agtgacaggt agcttaactg acgaaatgat
     4501 ggacagttac ttgagtgctt gcgtgaaaaa agaagtacca gaagcattgg aaacattgaa
     4561 ccaaattctc gcagccggca aagaatcacg tcgctttcta gaggatatgt tacaatactg
     4621 ccgtgacttg ctgatgtatc agcaagcacc taacctagtt gaggaacaat cgagtacgat
     4681 cagaagtaca tttacagaat tggcacaaac tgtttcagcg gatcagctgt tttcaatgat
     4741 aaagattttg agcgatactc aaaatgaaat tcgttttacc aatagtgccg ctatttattt
     4801 ggaagtcgct acagtcaaac ttgcacagct accgtctgct tctgttcctt cagcagatgt
     4861 agcatttgca gatgatcaga tagtaaaaca gctacaacaa gagattaagg aactaagaaa
     4921 cgaaatcaag gaactacgca gtaatacagg aattgagaat acaaaagtac agccggtaca
     4981 gcaaacaaat cataaaaaac agacaagtac ctatcgtgta ccgacggaac gagtatataa
     5041 agttctgcgg gaagcgacaa gacagcatct gaaccaagta aaagaagtat gggaagactt
     5101 gctgatgagt ctatctgtca ctcagcgagc aatgcttaaa gcaagcgaac cagttgcagc
     5161 tggacctaca ggactagtga ttgcgtttga ttatgagatt gtttgtcaaa gagcagcaaa
     5221 tgacgaagaa ttgcagcttt cggtacacaa caacttaagc cgaatgatca aagattatgc
     5281 gcctgatcct gtttatatta caagagaaag ctggccagaa cttcgaagaa attatttgac
     5341 gcaggcacaa gaagagggaa cagaaaactc ttttgcttct gaagactcag aaggagaaat
     5401 cgagttgctt cctcaagaac aagaagtacc agaagaaaca cttgtagtaa aagcagagga
     5461 actgtttggc tccgaagctg ttacagtggt agatgattaa taaaaaggag tagataatta
     5521 tgatgcgtgg aatgggcaat atgcaaggca tgatgaaaca agtacaaaaa atgcaaaaag
     5581 aaatggcaga agcacaagag ctgctaaata aaacagagtt tacaggtaaa gccaccaatg
     5641 aattggtcaa agcggttttt acaggcgatc gtcgcatgaa agatatccaa atcaatgaag
     5701 aagtcgttga tcctgatgat atcgagatgc ttcaggatct tgtcgtaatg gcagtcaatg
     5761 atgcgatcgc acaaattgat gtggaaactg agaaaaaatt agggaaatac gcaaaaggtt
     5821 tgccaggatt ttagttttaa actgaaggag taatgaatat gcaatatcca gaaccaattg
     5881 cgaaactgat cgacagctac atgaaacttc caggaatcgg tcagaaaaca gcgacaagac
     5941 tcgcctttta tacgatcgat atgaaagatg aagtagtaaa tgaatttgct aaatctttgt
     6001 taagtgtaaa acgcgatttg catttttgta gtatctgcgg aaacatcaca gaagaagatc
     6061 cctgcgaaat atgtagaaat ccttcacgtg atcgaagcgt tatcctagtg gtcgaagagc
     6121 cgaaagatgt aatggcaatg gaaaaaatgc gagaatatca cggactttat catgttctcc
     6181 atggtgtgtt atcaccgatg gaagggacag gtccagaaga catcaatatt tcttctctgt
     6241 tgcagcggct gcatgatgca gatgtaaaag aagtgattat cgcaacaaat gcaacaacag
     6301 agggagaagc aacagctatg tatctctcac gtctgatcaa acccgcaggt atcaaagtga
     6361 ctcgtctagc acatggtctg tctgtaggct ctgatatcga gtatgcagat gaagttactt
     6421 tattgaaagc tgtcgaagga agacgtgagt tataaatttc taattaagtg ttttttcaga
     6481 tacaatataa aagcagcaca agagatgggg gcaacaatgt gaacggacta tttattacaa
     6541 tcgaggggcc tgatggtgca ggaaaaacca gcatcttgaa tgaactttac cctcttttga
     6601 aagaagtagc aaaaacagaa atcattcaaa caagagaacc aggaggcatt ccgattgctg
     6661 aagagatccg agcagtgatc cttgatccaa aaaatgatcg gatggatgaa cgtacggaag
     6721 cactgcttta tgcagcagca agaagacaac atctagtcga aaaggttctg cctgctctgg
     6781 caaaagggaa aatcgtttta tgcgatagat ttgtcgacag ttctcttgct tatcaaggtg
     6841 caggccgacg catcggcgtt accgagattg ctcggttgaa tgagtttgct acagaaggta
     6901 ctacgccgga ttttaccctt tatcttgacg tagattcaga caccggatta agaagaatca
     6961 agaaaaacag acaaaatcaa atcgaccgtt tagattcgga aggtttggaa ttccaccagc
     7021 gtgtacgtca tgcttactta aagctggcag aagagaatcc ggaaagaatc cacaaagttg
     7081 atgcaagaaa aagctttgaa gaagtgctgc aaacaagtta tcatacaatt attgaacagt
     7141 atccgcaatt ttttgaaaac taggaaggtg aacgtttatg aaaattattt tagcgattat
     7201 ccaagacaaa gacagcaacc gtttatcaaa tgaattgatt gatgcaaata tccgtgccac
     7261 aaaactatca tcgacaggcg gcttccttaa ggctggaaac agtacgttta tcgtgggtat
     7321 tgaagatgac cgtgtagaag aagcattgga aatcatcaaa aaaacgtgtg aatcaagaaa
     7381 gcaatttgta tctacacctg ttacactgga tatttcgatg gatgggggcg tcccttatcc
     7441 agtagaagta gaagtaggcg gtgccacagt cttcgtctta cctgttgaag gattccatca
     7501 attttaggag gagctgagct atgaatcaag aaataaactt ggctcaaatg caaccgatcg
     7561 tgtatcagca gcttcaacgg agttttgagc atgggcgtat cgctcatgct tatctttttg
     7621 aaggggagaa aggaacagga aaacatgaag taggtatatg gcttgcacag cacctgttct
     7681 gtacccaaat gaaagacaat ctgccttgtg gaaaatgcaa caattgtcag cggatcgaaa
     7741 agaaagaaca tccagatgtt ttggttattg aaccggaagg acaaaccatc aaagtcgatc
     7801 agatcagacg tttgcagacg gaattcagtc gcagcggtta cgaatcaaga aaaaaagttt
     7861 ttttgatcaa agaagctgaa aaaatgaata gcagtgcggc aaacagtttg ttgaaattct
     7921 tggaagagcc ccctggagat tttttagcgg tgttagaaac agatgcagta ggaagaattc
     7981 tccctactat tcaatctcgt tgtcaagttc tgcattttca agaactttcg aaagaagcac
     8041 tgatccatcg attgcaagaa gagcagattc cattggaaaa agcaaaactt ttggctttct
     8101 taacaaacag tttaggaaaa gcagttgaaa tctctcaaga tgaatggttt aatgatgcta
     8161 aggacttgat ttatcaatgg ttcgtttact tgcaaaagca agatactcaa gcctttatct
     8221 atgtccaaaa aaaattggta aaatcattta aagaaaaaac acaacagttt acaggattat
     8281 ctgtcttgat gttttattat caggaagcac agcgtcaagc agtagcaaac ggcgaatatg
     8341 gcaaagtgaa acagataaac cagataattg aacggatatt gatcgcagag cagaaactac
     8401 gcagcaatgt cagttttcag gctgttgcgg aacagttcgt tttgcatacg attttttagc
     8461 ttgccgcaaa ggatgatgat caaggaggat ggaaatggta gaagtagtag gtgtccgata
     8521 tagagatgca ggtcatatct attattttgc accaggagac tctgaatatg tttatagtga
     8581 caaggtatta gtcgaatctc agcaatcctt acatattgct actgttgtcc ttcctaaaaa
     8641 agagattgat cccaatgacc ttcctgaagc tatccgtccg atcttgcata aagcgtcaca
     8701 agctgaatta gaaaaagaca aaaaaaacaa aaaagatgct tatgatgcat tgaaaatcgc
     8761 aaaagaaaaa gtgcagttgc gtgagttgga aatgaaaatg gtcaaagccg aatacacatt
     8821 tgatcgaaat aaattgattt tttctttcac agcggacggg cgaattgact ttagagaatt
     8881 ggtaaaagat ctggccgctg tatttcgtac tcgtattgag cttcgacaaa ttggtgtccg
     8941 cgatgaagca aagataatag gcggaattgg tccatgtggg cgtccattat gctgttcttc
     9001 ttttttaggc gatttcgtgc ctgtatcaat caaaatggca aaagatcaaa gtttatcttt
     9061 gaatccaaca aaaatttccg gtttatgcgg acgcttgatg tgctgtctgc aatatgagag
     9121 cgaagaatac gagacagcga aaaaagaact ccctgacttt ggaaaagtaa ttgctacacc
     9181 tgatggtaaa gggaaagtcg tagggttgaa tctgcttagc cgtatcgtta gtattcggct
     9241 gactggacgg gaaaatcctg tcgaatatga atgggatgaa ttgaaagaat ttatccaagt
     9301 aggtgagatg aatggataag agatcattat acgacggatt gaatcaatta gaatcagagt
     9361 tgagaaatac attaacagaa cttgttgaaa tgaagcatgc tttgcatgag atcgtagaaa
     9421 aaaatacaac tttggagatg gaaaatcaac gtctgcggga acacctgcgg gaattgaacc
     9481 aagttgcaca agctccagct gaaaatgtga agcaggaact ttctaagtct cgtatgaatc
     9541 ttgaaaaaat ttatgaagaa ggattccatg tctgctacga tttatatgga tcaagaaggg
     9601 acaatgacga gccttgtgcg ttttgtctag aagtgatcta tcgcgaaagc ggtcgatgac
     9661 caataacaag aggagactgg agcggagaaa tactgctcca gtctttttat taaggagatt
     9721 gtgacagatg tataatcaaa aaagtttcaa gggaaatccg gtatatgggg atttatatct
     9781 tgtaccgacg ccaattggaa atttagaaga tatgagctac agaagtgttc gtatgctgca
     9841 agaatccgat cttatcgcaa gtgaggatac gcgtaataca caaaagttat tgaatcattt
     9901 tgaaatccat acaccccaaa aaagtttaca tgaacataat tataaagaac gtgtccctca
     9961 attgattgct ttgttaaaag aaggaaaaac gattgctcaa gtcagtgacg caggaatgcc
    10021 ttccatcagc gatcctggac atgagctagt tttagcctgc ataaaagaag ggatttctgt
    10081 gatctctatc ccaggaccta cagctgggat gacggctttg attgcttcag ggctagtgcc
    10141 tcaaccattt ttattttatg gcttccttcc acgtaagaaa aaagaacaga aagaaacatt
    10201 gaaaaagcta aaagaacaga cagttacgct gattttttat gagtcacctt atcggatcac
    10261 tgctacattg acgaatttct tagaaatttt tggaaatcgc caagtcgttt tgtgtcggga
    10321 attgacaaag gtccatgaag aataccttag aggaagtacg gaagagctac tcgcttatat
    10381 cgaagaacat ccagtaaagg gtgaatgttg tctgcttgta gaagggaatt tgttcgaaat
    10441 gccagtgacg gaagttgatg aagagttggg aacgttaaag gaacaagtag aagcaaagat
    10501 cgcttcaggc gaaaaaccaa atgctgctat caaagcagta gcgctgaaaa atgggctgaa
    10561 aaaacaagaa gtctaccgtc agtaccatga attagactga gatagtataa gttgtctata
    10621 atgaaagact aatgaggaaa tcagctacta acttttcaaa tctcttgaac gcgaacgaat
    10681 gttcttgtat aatgaataag aggtgaggat gatggagctg caatttcctt tacataatga
    10741 tacatcaaga aaaatcattc atattgatat ggatgctttt ttcgcttcgg ttgaagaacg
    10801 agatcatcct gagtttcgcg gacatccatt agtaattgcc agacacccca gtgacactgg
    10861 tgggcgtgga gtagtgacta cagcaaatta tgaagcaaga aaattcggaa ttcattcagc
    10921 aatgagtgca caaaaagcgt atgagctttg tccgcaagcc atctttaagc ctggcgacca
    10981 tcataaatac agcgatattt ccaaacaagt acgggaagta ttctatcgtt atacagatat
    11041 tgtggaacca gttagtatcg atgaagcgta tcttgatgtg accgaaaata aaatcaattg
    11101 caagtcagcc atcaaaatcg cccgaatgat ccaagcagat atttgggaga gtgtccagct
    11161 aacatgttct gcgggagtaa gttacaataa gtttttggcc aaattagcat cagactatca
    11221 aaaacctaga ggaatcacgg tagttgcccc tcaagacgct gtgccatttt taaaagcttt
    11281 gccaattgaa aaatttcatg gggtagggaa gaaaactgta cctagaatgc atgagctagg
    11341 gatttataca ggaaaagacc tatatgaatg cacagaaatg atgttgattc gaaattttgg
    11401 aaaaatgggc tattctcttt atcgtaaagt cagaggaatc catgattctc cagtaaacgt
    11461 tacaagagag cgaaaatctg tgggaaaaga gcatacttat gggcagccgt tgcaaacgga
    11521 agaagcagtg ctgactcagt tgcgccaact agctgaaaaa gtagaagaag cactgagaag
    11581 agtccaaaaa catgggaaaa cagtagtttt gaaggtcagg tacacggact attctactgt
    11641 gaccaaaaga gtgacattgc cagaatatat ctataaaaaa gaagcactct tttatcaagc
    11701 aagccttata tgggaggaaa tcttgggagt agaaaaagga atccgattgt tagggattac
    11761 tttgacgaat ctggacccga tgacttatga aaacatcgtc cttccattat gggaaaatca
    11821 agaaatataa aaacagcttt gagagttgaa accggaaatc cgttttctct cccaaagctg
    11881 tttttatatc aagtcttgct ttttgatttg ttcataggtt tctgttgcat ccgtacattt
    11941 ttcccaaatc acaacttgat tggattctaa agatttttgg acgtcaatga tccgctggtt
    12001 gctacttccg cgaaattgta gttttaggtc acgtttgctt aattcaaagc gtccgtcaac
    12061 aaggatatcg atttgcgaaa ggagttctaa tttatcttct gaatcttgta gcaattcatc
    12121 aaaggtatag cctgtccatg accagatatc ttttgtcgta ccgaactctt ttcggatacg
    12181 tcgtacgact tgcagacaaa catctgtatt caaaaaaggt tcgccaccaa gtaaagttaa
    12241 tccttgcacg taatcgttgc gcaagtcagc gatgatttgt tcttctaatt caggtgtgaa
    12301 tggtttgcca tatcggaaat tttgtgcagc tttgttgaag cagccttcgc aggcaaataa
    12361 gcagccacta acgtaaagac tgttgcggac accttcacca tcgacaaaat taaacggttt
    12421 gtagtcagca atatagtttt ggctgtagtc ttttgctaac cattcttttg gttttggatt
    12481 tctcataaaa aaacttcttt catttaaggt tcaaagatat agctattgaa gataaaatct
    12541 actcgaccac ataaataaga acaaactgac ctaaatccat tgttcaagaa agcttgttct
    12601 tattttgata gagggcgttc aaagatacaa agccatcgaa agagggtggt gaaaaaagac
    12661 ttgtcatagc ctcttattct gaataaacgg tgttcaaaag cttgcatctg cagtaataag
    12721 cccaactaaa cgaaaaatat gaggaactat ttttgtttag ctattcttat tacttgatgc
    12781 aggacagctt tttcacaacc tcatttcatg tgtttcacac gtgaggaaat ttctttatgg
    12841 cggccatgta ccatcggacg agcttgcgga tttcctaaat agccacaagt acgtttgact
    12901 acatcacatg tttttggatc atgatttcca cattctggac attcgaaccc tcgttctgtc
    12961 ggtttgaaat caccttcgaa accacattga tagcaatgat cgattggcgt attcgtacca
    13021 agatacccga ttttgtcata tgcaaaatcc catacagctt ctaaagcttt cggattttgt
    13081 tggagcatag ggtattcaca gtagtggatg aatccacctg aacaatattt tggataatcc
    13141 ttctcaaatt ccaatttttc aaatggcgtt gggtttttac gcacatcata gtggaaactg
    13201 tttgtataat attctttatc tgtgatattt tcgatgatgc cgaatttttc tgtatccaaa
    13261 cggcagaaac ggtcagttag actttcactt ggagttgaat agacactgaa atgataacca
    13321 tattcatccc cccatgcatc tgcattagct tttaattctt tgacgatatc aagtgtgaaa
    13381 gcttttgctt ctgggtttgt ttcccattcg ccaccgaaga aagctgaagc aacttcatac
    13441 aatccaatgt agcctaacga tacagtagct cgacggtttt tgaataattc attgacagaa
    13501 tcggtacgtg aaagacgttt cccgaaagca ccatacatat ataaaattgg cgcatttgct
    13561 ggaatggctt ctttacaacg ttctacacga tagagaagcg catctttcat gatttctaag
    13621 cggtcagaca gtaactgcca gaatttattc tgatctcctt ttgattcgat cgcgattctt
    13681 ggcaaattca atgtaacaac acctaaattc attcgtccga catttacttc ttgaccattt
    13741 tcgtctttcc acccttgcaa gaatgaacga cagcccatag gtactttgaa acttccagtt
    13801 aattcaacga ttttatcgta attcagaata tcaggataca ttcttttcgt tgcacattcc
    13861 aatgctagtt gtttgatgtc gtaattagga tcttcagggt ttaagttcac accttttttc
    13921 aaagtgaaga tcagtttagg gaagatagct gtccgctttt cactccctaa tccattgatt
    13981 cgaatctgta aaattgcttt ttgaatttct ctttcaaacc agttttctcc tagtccaaat
    14041 cctaatgaag tgaaaggagt ttgtccattt gaagtaaata aggtattgat ttcatattcc
    14101 aaactttgca tagcatcgaa gatatctttt ttggtttttg cttttgcata ttctttttgg
    14161 cgttctggcg aatcgatcca ttcttccgcg tcttttagat gttttttata gttcaattct
    14221 gcaaaaggag caagcaattc atctgttcgg tcggcagaac agccaccata ttgacttgac
    14281 gctacattgg caataatctg tgagatttgt gctgtggcgg tctggataga ttttggcgat
    14341 tcgacttcgg cattgccgat tttgaaccca ttgttcagca tgcctttgaa atcaatcaag
    14401 cagcaattcg tcatcggtgt ataaggatga taatccaaat catggtaatg gatatcgcct
    14461 ttttgatgcg catttgctat atgtggtggc aacattttca gtccgattga ttttccaacg
    14521 atcccggctg tcagatctcg ctgtgtattg aaaacattac tgtctttatt tgcgttttcg
    14581 ttcacgacag tttggtcctt gttgattagc ttttcaatcg aaaagttgat atctgttgct
    14641 ttactgcgag caaaatctcg tttggtacga taattgatgt actcttctgc taattcatat
    14701 tcgttttttt ctagtaatat atgttccacg atgttttgaa tctcatagat tttgacattt
    14761 tcagcaaacc ggcttttgat ttcttggaca accaagtctg caatcatttg aattttttca
    14821 taagtaagtg gtgataatga gccgtgaatt ttcttttctg ctttgatcag tgcttcattg
    14881 atcttttgtt catcgaaatc aacatggcgg ccatcgcgtt tgatgacttt catctgggat
    14941 gtctctggct gctgagtaac gacagttttt tcttcataat ttactttcat tatcttcaac
    15001 tcctgctaaa ttgttcttgt ctatcataaa acaaaatcgg ggaaggagca actaaatatc
    15061 ggctatatat agtattcaat gtgctgtata attggatttc tatactatat gtagtgtata
    15121 ttttattgtg aaaaaaggtg aaaacttgta gctggtggat ttgttttttg catatttaat
    15181 tttgctgaat aaaataattg aataaaattt tttattcagt ccaagtaaaa gtgacaaaaa
    15241 cataagagca aattattcgt agatatgcta ctatgcaaat aaatagaaat gaaaaatgaa
    15301 agaggaggag aagaattgga aaagatgatc gaagtgaaac atctaagtaa aagctacggc
    15361 agcaaacttt ctcccgtcaa agccttagat aatatctcat tcacagtaga agaaggcgag
    15421 tttgttggga ttatgggccc aagtggtgca ggaaagacaa cgctgatgaa tatcttgtcc
    15481 acgatcaatt tgcctacgat gggcagtgtc tcgatccaag gaaaagaaat cacaaaaatg
    15541 aaaaacagtg agctcagtga ctttcgtcga aaaaaactag gattcatttt tcaagagttc
    15601 aatttgatcg ataccctaaa tgcaaaagac aatatcctat tgcccctagc agttgaacgg
    15661 atgactaaag aggaaatgga aaagagagta agacatgtag ctcaattgtt gaatatagaa
    15721 gaactgctca aacggtatcc cgatgaactt tctgtcggac agagacaacg aattgcagct
    15781 gctagagcac ttgttgttca gccacaagtg atttttgcag atgaaccaac tggctctttg
    15841 gactcgaagt cggctacaga attgttgaac tacttgaaga ccatgaatca aaaagaaaaa
    15901 gcaacgatac tgttagtcac ccatgatcca tatacagcaa gctactgcga tagaatttta
    15961 tttattaaag atggtgtgat tttttctgaa gttgtcagaa gagggacaag aagagagttc
    16021 tttgagaaag taatagacat gcaggcgacg attggcggag gaggaaaaat gaatgctgta
    16081 taaactagcc gttaaaagtt tttttcggca gtcacgaggc tatcttgttt attttttcag
    16141 tttgacttta tccgtaatga tctattattc cttcagcgca atgacctacg atcaatcttt
    16201 agtcagaaga gcaagtcaag atacttcgat agatggtgga ttgagttttg gtggatttat
    16261 cattactgtt gttttgcttt ttttcgtatt cagtgccaac cgatttttct tgaatagaag
    16321 gcaaaaggaa ataggcatct atcagctttt tggtatgaat aaactgcaga tatcaggtat
    16381 atatgtgtta gaaatcatga taattggatt gttcgcctgt atttctggga tattactcgg
    16441 tattattttt tccaaactct tttcaatgat tttggttcga atgatggata tggatcttac
    16501 tagtccattt tttatctcca ttccatcagt agtagatact ttatttgctt ttttcatcat
    16561 ccttttagca gtatctgtat attcaatatg gaaaatatgg cgctatccaa tgattcgaag
    16621 ttttggagaa aaagaacaag cagatagttc aacaatgcgc ttcaggacaa ggcaccgatt
    16681 actcgcagtc atcgggttac tacttttaac gagcggatat tttggagcta gtcattttag
    16741 ggaaatcact tcatggatga tcagttatca ttatcttgat acattagttt tcttttttcc
    16801 ttttttaatt tttttcgttt gtgtggtcgg tacttatttg tttttctgtt atacatttcg
    16861 gttatttttg atcatattga gtaaatggaa gatttattac tatggtatca attttttgat
    16921 aatgggtaac acgcaagttc atttgctcaa aagttggcgt acaaattctc tgattacagt
    16981 cattctcgga atttctttaa tgacaattgg cggcacggct agtatctcta ctattttatc
    17041 ctaccaggag aaagtttcct ccccgatgga ctatcagttt gatgttgaga cagagaaaaa
    17101 aatcaagccg attctagcag aggaagggca gaagataact aaagaaatca ccttgcatta
    17161 taaagtcatt gggagtatct atagtcaaaa tctcgaagaa cctatgtttc aaactgctaa
    17221 tctgatcacg gaaacacaat atcaagcctt cagaaaagtt aatccagatt tgccgaacat
    17281 ttctttgaaa tcacctggtc atactgtttt gcttgatcaa atgcaaactc tttttcggaa
    17341 tatttcatta tatggatcta gtattggact gccagataat caagaattga agattcaaca
    17401 aatgcttcca agtgtattag gtgacgaaac aatggtatat ctaagtcctg tactgattgt
    17461 agatgatacc gtttttgaaa aagcaaaagg tgtggattat caagtagtca atgtagatgt
    17521 atcaggtgga aataatgaaa aaatagatga acgactgagc aatgacctgt cgataaagtg
    17581 gctcaatgct gtttattatt cttatgatat cgtaaatgga agtctacaag gagaaattag
    17641 cacaaagaaa cttccggatg accaaatgaa ggaagcacaa tttagccaag aaagctctcg
    17701 tttgaatttc tcttccagct attcgaaatt gcgtgcagtt aatcggcgaa tcggtatcaa
    17761 tatttttgtc acactatttg tcgggatgat cgtgattatt gctactggta gtattttgac
    17821 agtccggcaa ttagcagaag cagaggaaga aaaagaaaat tatcacctat tgactaagct
    17881 ggggattcca caaagaagga tcagacggat gatcttcgag caaaacgcac ttaccttctt
    17941 tccacccatg atattaggga tcactcacgc agtatttgca atcaatgtct tcactcagta
    18001 tataaaaact gccgattact ggttggctta tcttgtgagt ggcttattga tccttatcta
    18061 tcttttgctt tatgtcatta cgagtcagtt atattgtcgg attatcgagg aatgatatac
    18121 ggaataagac aagataataa atagaactaa attttgaata ccatttattt ggaataagag
    18181 gttgtgacac gatttttgtc acaacctctt tattttttat attttttaaa agtacgagaa
    18241 attataaaga aaaaagcagt gatttgatgt gctattttta taaaagctat gtgacaaaac
    18301 tgttagtaaa aaaacggttt ctgtaagcta gacacatgtt tctttttgtc aaaattagtt
    18361 acactttgtg tgaggttata tgaggaggga atccaatgaa caaatggcgt tctaatccaa
    18421 aattgaatta ttggggaaaa ttcatcattc gaacaatttt ttatagtgca gtactgctgt
    18481 tcctgattta tctgtatcac tataaaaata ttcaaggagg aacttttatc tataacgaat
    18541 tttaggagga agagtatgga aatcaaaacg attattgaag caatcgatga ttggggaata
    18601 aatgaacctg atcgagttgc ttatcaggcg gatgagacgc atacttttgg tgaattgaaa
    18661 caagcatcag acgcacttgc ttattattta gaaaaaaagg tagaaggcga tggaccaatt
    18721 gtcgtttttg gcaatttgga gtttgaaatg attgtctcat ttctaggggt tgtcaaatct
    18781 ggccatgcct atttgccgat tgaagagcat acacccaagg agcgtatcct atcgatcttt
    18841 cgggtggcta aaccatctat ggtcatcagt atcggggact ggatagaaga tattccttcg
    18901 gttcctgtca taacaaaaaa agaatttcaa gaaatcaaga agataccagt aggattttat
    18961 cccagaaata gcgtaaaagg tacagaaaat ttttatatta tttttacttc tggtactact
    19021 ggagaaccaa aaggcgtaca aatcagtcat gataatctag tcagctttgt tcaatggacg
    19081 atggaagact ttgggatcaa gcctggtatg catttcttag cccaagcgcc attttctttt
    19141 gatttatcgg tattttctat ttatcctgca ttagtttctg gcggaatgct gaagccgtta
    19201 aataaagcag ttgttcaaga ctttcgtcaa ttattcgcga cattgccagc cttgaagttg
    19261 aatgtgtggg tttctactcc atcatttatg gatatttgct tgatggaacc tacattcaat
    19321 ggagaaaatg tgccggaatt ggacatgttt ttattttgcg gagaagaatt gacgaaaaaa
    19381 actgcagaaa gtctacttga tcgattccca aatgcacgga tatataatac ttacggtcca
    19441 acagaagcaa ctgtagctat ttcaagtatt cagattgacc agcatgtact agatagttat
    19501 gaccgactac cgattggcta cgtaaaaaaa gacacgaaag tttctattgt ccaagaaggt
    19561 aaacctgttt caaaaggaga aacgggtgaa atcattattg caggaccaag cgtatcaaaa
    19621 ggttacctga acaatcccga aaagacagct gctgcgttct tttgtcaaaa tgacttagca
    19681 agttaccata caggggatgc aggtagacta gatgaatatg ggcttttatt ttatgaagga
    19741 cgtatggatc agcaggtcaa attacatgga taccggattg aactcgggga tatcgaacat
    19801 tatctgctcc aagacaaccg aatcaaacaa gctgtcgtcg taccaaaata ccaaggaaca
    19861 aaagtacagc agctggttgc ttttgtcgta ttagaaaaca aaacgaaaca gccggatttt
    19921 cagttgacta aatctatcaa agaacaatta ctagaagttg taatggatta tatgattcct
    19981 cagaagttcc attatgtcga tctgctgcct cagacagtta atggaaaaat cgatcgaaaa
    20041 aaattaacag ctgaggtgaa tcctgaatga tgttacctca tatgatccct tatgcagagc
    20101 cgttttattt ttttctttta gccattgcgc ttgtaccgat cgttctatct ttattgatat
    20161 gggaaaaaag attaccagtt tatcaatcag cagtgacact tttcttttta tttgtcagct
    20221 ttggaggtaa ttattggcaa cagggagcgg cgctgattgg gtatgttttg tggcagacga
    20281 tacttgtatg gggctatttt gtttatcggc aaaggaaaaa tcagacagga tggttttttg
    20341 ccgccgttat tttagcgata attccactgt ttttaacgaa agtgactcct ttttttgaac
    20401 atggaaaagc atcgttactt ggttttttag gaatctctta tttaaccttt aaatctgtac
    20461 aaatgatcat ggaaataaga gatggcatga tcaaaacata taagccattt cgttatatcc
    20521 agtttctgct gttctttcca acaatctctt ctggaccaat cgatcgctat aggagatttg
    20581 aaaaagatca gctccatccg cctgttcgtg ataaatatgt tgatcttttg ggaaaaggcg
    20641 tccaaaatat ttttgtcggt tttttatata aattcattat cggttattat tttggtcaag
    20701 tactgcttcc tgtagtggga cgcattgcct tacagcatgg tggtatttca tgggcattag
    20761 taggttatat gtacgtgtac agtatgtatt tgttttttga ttttgctggc tatagcttat
    20821 ttgcagtcgg tacaagctac atgatgggat atcagacacc aatcaatttc aataaacctt
    20881 ttttatcatg gaatatcaaa gaattctgga atcgttggca tatgacgctg tccttttggt
    20941 tccgtgatta tgtatatatg cgattgatgt tcttcatgat gaagaaaaaa ttttttaaaa
    21001 gcaagatcgt aatgtcaaat atcggttatt ttgctttgtt tcttctaatg ggtatatggc
    21061 atggattgac gtggtactac atcgtatatg gactgtatca tgcttgtctg atctgtctga
    21121 cagatgcatg gatccgattt aagaagaagc ataaagagca gttgccttct aacaaattta
    21181 cgcatggctt agccgtattt ttgacatttc aagcagtatg ttttagcttc ctgatttttt
    21241 caggattttt agataaactt ttttttagct aacgaatagt gacatattaa tttatataac
    21301 aaaggagaaa aggaaaatgg aagaacaaat tttatcaatc ttagaagaaa taacaggaac
    21361 agatgaagta agaaaaaatc gtgatattga tctatttgat gaagggttgc ttgattcttt
    21421 agcatctgtt caattattag tcgagctaga cggagagctg gatatccaag tccctgtatc
    21481 tgaattcgag cgagaggatt ggagcacacc gaataaaatc attcaacaag caactgcatt
    21541 gaagggttga gtatgaaaaa gaaacttttt gctattttcg gcccggttgt tttagctgtt
    21601 ggcttactgt ttgtgttttt tacctcctcc tatcaagtag atatctctaa tccagaaacg
    21661 attagacgtg cctcttcttc catgtcagtg aacgtgttga aaggagatgc gataaaaaat
    21721 tatgcattat ctgaaaaaca atatattcct ttttttggct caagtgaatt aagcagaatc
    21781 agtccatttc atccgtctgt tctggcagaa aaataccagc gaaactatcg tccatttttg
    21841 ctgggagcac ccgggacaca atcgttaagc caatatatga tgatgcgttc tgcaggagat
    21901 gctatgaaaa acaagaaagt cgtctttatt atttctcctc agtggttcgt taaaaatggt
    21961 gtaaaaactg attactttaa tacgtattat tctgaacttc aaacatacga ctggttgttt
    22021 tcaatgaaga aagtaacgcc tgctgatcgc tatttagcta gaagattatt aactttctca
    22081 aaagtaaaag agaatgatac gttgacggcg attctgcaaa cgatcaaaaa agggaagcta
    22141 cctttacctg aatctttgaa tcagctgcgc agccaatgga acatgctgaa gagagaggat
    22201 gaagtattca gcaatattgg gttgaaagat cgacagcaaa aaatcgatca tgagtcaaaa
    22261 cgattaccaa aacaatacca agaaacagaa ttatccattt tagcaaatca aattggagaa
    22321 cgagaaacga cgaataatcc attcgggttg aaaaacgatt tttatacgca tcgtatccga
    22381 gcccacgagc cagagctcaa acagtcacaa aaaaattggg attatcgttt ttcgccagaa
    22441 ttttctgatt ttcagctggt cttggatcaa ttagcaaaaa accataacga agtattgttc
    22501 atcatcccac cggttaacga gaaatggagc gattataccg gactatctca agaaatgtta
    22561 caaggatttg ccaaaaagat caaatttcaa ttaaatagcc aaggattcaa tcgaatagct
    22621 gattttgtca atcaagcggg taccaactat tttatggaag acacgatcca tctaggctgg
    22681 aaaggctggt tggctgcaga tcaacagatc agaccgtttt tagaagaaaa tcatattact
    22741 gctagtaagt atcatcttga cgatgctttt tttagcaaat cttggcaaca tcaaatacca
    22801 gataaactac aattaaaata aagaaaacag gcgagtgttg ctcgcttgtt ttctttattt
    22861 ttgaatattc tgaataaaag ataaaaatac caagtttgcc tgaacaaaaa tcataagcaa
    22921 tggtataatc acaatatgaa tatagaaatg ggggaaaaca tttggataca aaagaagaac
    22981 agctacttat tgatttaact tctgcaaaag gagtacctgg aaatgaagaa gaggtaagag
    23041 aagtattcag agaatatgcc aaaccattcg ctgatgatat tttttatgac ggtttaggca
    23101 gtgtgattgc caaacatggt gctggcggag gccccaaggt ctttatttct ggacatatgg
    23161 acgaagttgg ttttatggta acaaaaataa ccgaaaaagg attcttggaa ttccaaactc
    23221 ttggaggttg gtgggggcaa gtcatgcttg ctcagcaagt tgaaatcaaa acacgtgaag
    23281 ggaaaatcgt ccacggtgtg attggttcca aaccaccaca tgttttaaca cctcaagtaa
    23341 gaaataaacc ttatgaaata aaggatatgt tcattgatat tggcgcatca agccaagaag
    23401 aagccaaaga gtggggcata cgtcctggag atatggtcac tccatatatt gaatacaaac
    23461 gaatgaatgg cagtaaatat ttactggcaa aagcatggga taatcgtatc gggacagctg
    23521 tttctctgcg agtattagag aatctatcta aagaagcaca cccaaatgtt ttattcgcag
    23581 gaagcgatgt gcaagaagaa gtaggcttgc gaggtgctcg aacaagtaca caccttgtaa
    23641 atcccgatat tgcttttgct ttagatactg gtacagcagg agacactcca ggaatgacac
    23701 caaaagaagc agattcgatt ttaggaaaag gcccacagat tttgattttt gatgcatcta
    23761 tgatcccgca taaaaaatta ttgaattttg ttattgatgt agcagaggaa atggatattc
    23821 cattccaata tacagtgatt tctggcggag gaactgatgc aggacaaatg catttgacaa
    23881 gagatggtgt tccgtctgta gccattaccg ttcctgttcg ttatctgcat tcccatacat
    23941 cagtgattca tgaagatgat tatttgaata cagtgaaact agtcacagaa ttagtgaaac
    24001 ggcttgatag tgaaacagtt gaaaaaattc ggagttatga ataatgaata atagaatacc
    24061 tatttctgaa tatcggaaaa aagcacgagt ttctttggaa aatcaatggg gaatcaatac
    24121 ttggcttgtt ttcttatcta taatatttac gagcgtcatc cagaactttt ttgacggttt
    24181 cagtcctttt ccggaagaaa gcatgcagca gcacttattg agttttctag tcgataattt
    24241 acttttattc gcattcaact atgcattgtt ttacattgcg ctagtagttg tacggggtgg
    24301 cagaccaaaa agtaaccttt tattttcagt tttccaaaga aagtattttg gttcgatact
    24361 tggcatcaat ttgatcaatt cagtcatcaa ctggctactc aacagtttaa tctttctgcc
    24421 catgttttta atcagcggag tgagtactta tatgcagctg cttttcagtt caaatgcggt
    24481 acctacgggt gtcatgccag aagattttct tgatatcagc tttgttttat cgatgatagg
    24541 gatgattttt gtttcagcac tcatcatgag tattgttggc ggtttattcc aatttgctgc
    24601 atggacaaag ttcgactatc ctgatttgtc ggttattcaa tgtttgaaat atggctggtt
    24661 tttattgaaa gatagaattg gtacgtatat tttgttgcag ctgtctttta tcggctggta
    24721 tttgctagga gcaatcgctt tgttcatcgg tcttttgtgg gtcattgttt atgtgaatgt
    24781 gtcagttgca gaattttatg aacaagcacg gattgaaaaa ggaggtccag acgcctattt
    24841 tacttagcat gtaagcagtt tattcatcaa aaaaataact tttgtggtag gataaatctt
    24901 tgagggggaa tgtacgatga aattaactgt tttaggatgc ttaggagcgt atccttataa
    24961 aaatcaaggg acaacaggtt atttgcttca atctgacggg ttcaatttgt tgatcgatgc
    25021 aggtagtgct acgctgatca aattacaaga acaccttgat ccacttgatc tagatgctgt
    25081 gatccttagc cattatcatc atgatcatat tgctgacctt ggtgtattac agtattactg
    25141 gcaacttcat cctaagagaa atccggagac attgctttca atttatggac acacaaaaga
    25201 ttccattcac tttgaagaat tgacgttgga aaatgtctca aaaggacatc cttattttga
    25261 agctgaagaa ttaaaaattg gtccttttct gattactttt atggaaacaa tccatccagt
    25321 agtatgttac gccatgcgta tcgtagaaga aacaacgggg aaagtattcg tatttacagg
    25381 tgattcaggt tatttggaaa gtttcgctga ttttgcgaaa gatgctgatt tgtttctagc
    25441 agatacctat ctttttgaag gaaatgaaca tcatcatgcc catttcactt caaaagaagc
    25501 tggagaactt gcgaaatcag ctaacgtaaa gaaacttgta cttacacacc tacctcagtt
    25561 tggtgattta gaacaattga aaaatgaagc gcaaaaggca gcaggcaaag ggattgatat
    25621 agaacttgct gcagttgaca aagtgtttaa catttaggag gaagaacatg atttttgtac
    25681 caaatgaaaa ccacgaccca agaatcaatt tagcaattga acaatatttg ctgcaagaaa
    25741 tgcctttaga tgaacctatt ttgctctttt atatcaatga accttcaatc atcatcggca
    25801 gaaaccaaaa tacgcttgaa gaaatcaatc gagattatgt ggaagaaaat ggcattcatg
    25861 ttgtccgtcg cttaagtgga ggcggagctg tctaccatga ttttggaaac ctgaacttta
    25921 gttttatcat gcctgatgat ggggattcat tccgtgactt tgcgaaagta acacagccaa
    25981 tcatccaagc acttcatgaa ttgggtgttg ctggggcgga attgaaaggc cgaaatgatc
    26041 tagtcattga tggcatgaaa ttttctggca acgcgatgta tgctaccaac ggtcggatgt
    26101 ttgctcatgg aacaattatg ttcgactctg atatcaatga agtagtcaat gcattaaaag
    26161 taaaaaaaga caaaatcgaa tcaaaaggaa tcaaatcgat ccgttctcgt gtaacgaata
    26221 tcaaaccatt tcttccagaa gaaaagcaaa aaatgacaac agaagaattt cgtcaagaaa
    26281 tcttgttgaa aattttcggc gtagagtcag tagaagaagt aaaaacgtac gaattgactg
    26341 atgaagattg gaaaaaaatc aatcgaatct ctgaggaata ttatcgaaat tgggattgga
    26401 attacgggaa gtctccagat ttcaatttct ctcgccaaaa acgtttttct atcggttcga
    26461 ttgaagtcca tttgaatgtc tcagaaggaa tgatcaaaga agcaaaaatc tttggcgatt
    26521 tcttcggact aggagatatc tctgacgtgg aaaaaaaatt ggttggacag aaatatgata
    26581 aagcatctct atcaaaaata gtcagccaaa ttgatgtgaa gaaatatttt ggtgcgatag
    26641 aaccagaaga attgcttacc ttactttatt gaaaataaaa aatagcggaa tccacatata
    26701 tggattccgc tattttttat acaggattcg ggtaatttcg agagagttga acagtgatcc
    26761 gatcaatgaa agtatctttt tcctgcgtta gtgttcctgt gtaacgatcc gctacgtctt
    26821 tcatcatcat taagtcaaaa cgttcctgat catctaattc ttctgtgatg cctcgactaa
    26881 ataggtttgt atcgtaatgt gcactatgtt cgcaacaaat gacgaaatgt tcctcttgtt
    26941 cgtgtatcga caaataaatt tttcgataca gtggattttc gatggcatga gtttctctaa
    27001 aggaatgctc taaagcgtgg atcaaaagtt gagccagatc gtcatcgttg ataggaagtt
    27061 cttcgggaag atctgcttgg aaggttatct gaatattccg tttggcagct aacttctggt
    27121 aacgcgctaa gataagattt gtcacctggt ggttgcaaaa agaacacagc atttcgaaat
    27181 tttttgcttc ttctaatatc ttttccacat agtttttagc ttttgtttgg tccgtatgca
    27241 ttaaatcttg cagattctcc atctcttgga caaattccag gcgaagtgta tttacttctt
    27301 ggatatgctg tcgaagctgc tcataatgtt gctccaacaa atcatttttg atttttaaga
    27361 aatctacttg tctccgatct ttgtcggtat ttaagatgtg atcgattaga ttgtagctga
    27421 taataacgat tagaatgata atatataaaa tcgtagacca attgatcgtt gtttgataga
    27481 tacctaatgc cgtttggatg taaatcaagc aatggataat ggctaccaag acgatccaag
    27541 gtgtacaagc tacataaaaa cggttattct tatatgcttc gccaatgcat gcaagtgaag
    27601 tgactaaagt gcttataaca ctagcaatat tagcaatcgg cattaattca ggaagttctg
    27661 caggagtaaa agttgcataa agcagaacgc cgataaaaat caaaagttgg aacgtacaaa
    27721 aaattccgta ccatttttta tagtaaaaca ttcggctcca atagtagcag atgatgaaag
    27781 ctgacgttaa gataataaat aaatgagata aaaaagaatg aacgaatggt tcaaaaagaa
    27841 taccacttaa aatatcttct gatacagctt caaatcctaa tgccaaagca aagcaactta
    27901 agagaataag tgagtattca agacgttttt gtttatacat caagtagatc gtatagaaaa
    27961 aggtccctat agcgataaaa acagcgatta tcatcgtcaa tacttgcggc actgattgtg
    28021 aataaataaa actaatcaat ggtccagttg ttcctagata aactttcggt ggcagtccag
    28081 catagttctg ataaggagat gagactgtaa gggtcaattt ttttccgata taatttgctg
    28141 gtaaatctat cacagccaat gagagtccgg gattgctttc ttcctgataa tctttccgtt
    28201 ggtataaaat cttttcgcct agatatacag taatccattg atggtttcct ttgattagta
    28261 aatttgcttc gtccaacggt ttttcaagtg tcgtttccat aatgaatgtt tcgtctttat
    28321 ccacggtagg aatcgtacga gtgtacttgc tgtcaaactt aaaatcagga tcatcttttg
    28381 tatagaaaac ccacgaatct tttatttctt tcatttgatt gctgtccgca ggagaaaaaa
    28441 taaaataaaa gaaataagca attcctgtta aaaataacaa tgataatata atgattatgc
    28501 tccactttat acgtaaataa aaagattctt ttttattata tatgttatta aaaaaagaca
    28561 taaaggtatt cctttct
//
LOCUS       DAMEET010000030        28007 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.030, whole genome shotgun sequence.
ACCESSION   DAMEET010000030 DAMEET010000000
VERSION     DAMEET010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 28007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.030"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            99..269
                     /locus_tag="PB780_001168"
     CDS             99..269
                     /locus_tag="PB780_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733233.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVRVKSKRP
                     TNHTKKWMAKNR"
     gene            489..1196
                     /locus_tag="PB780_001169"
     CDS             489..1196
                     /locus_tag="PB780_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDE0733234.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(1255..1899)
                     /locus_tag="PB780_001170"
     CDS             complement(1255..1899)
                     /locus_tag="PB780_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDE0733235.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            2025..2312
                     /locus_tag="PB780_001171"
     CDS             2025..2312
                     /locus_tag="PB780_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733236.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            2420..2740
                     /locus_tag="PB780_001172"
     CDS             2420..2740
                     /locus_tag="PB780_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDE0733237.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            2821..4134
                     /locus_tag="PB780_001173"
     CDS             2821..4134
                     /locus_tag="PB780_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDE0733238.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            4131..4850
                     /locus_tag="PB780_001174"
     CDS             4131..4850
                     /locus_tag="PB780_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDE0733239.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            4978..5529
                     /gene="raiA"
                     /locus_tag="PB780_001175"
     CDS             4978..5529
                     /gene="raiA"
                     /locus_tag="PB780_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDE0733240.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            5751..8285
                     /gene="secA"
                     /locus_tag="PB780_001176"
     CDS             5751..8285
                     /gene="secA"
                     /locus_tag="PB780_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDE0733241.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            8411..9512
                     /gene="prfB"
                     /locus_tag="PB780_001177"
     CDS             join(8411..8482,8484..9512)
                     /gene="prfB"
                     /locus_tag="PB780_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDE0733242.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            9617..10303
                     /gene="ftsE"
                     /locus_tag="PB780_001178"
     CDS             9617..10303
                     /gene="ftsE"
                     /locus_tag="PB780_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDE0733243.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            10287..11180
                     /locus_tag="PB780_001179"
     CDS             10287..11180
                     /locus_tag="PB780_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDE0733244.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            11332..12780
                     /gene="cls"
                     /locus_tag="PB780_001180"
     CDS             11332..12780
                     /gene="cls"
                     /locus_tag="PB780_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDE0733245.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEIAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYILQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            13117..13974
                     /locus_tag="PB780_001181"
     CDS             13117..13974
                     /locus_tag="PB780_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDE0733246.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            14089..15009
                     /gene="pstC"
                     /locus_tag="PB780_001182"
     CDS             14089..15009
                     /gene="pstC"
                     /locus_tag="PB780_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDE0733247.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            15009..15893
                     /gene="pstA"
                     /locus_tag="PB780_001183"
     CDS             15009..15893
                     /gene="pstA"
                     /locus_tag="PB780_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDE0733248.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            15908..16714
                     /gene="pstB"
                     /locus_tag="PB780_001184"
     CDS             15908..16714
                     /gene="pstB"
                     /locus_tag="PB780_001184"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDE0733249.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            16730..17488
                     /locus_tag="PB780_001185"
     CDS             16730..17488
                     /locus_tag="PB780_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDE0733250.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            17501..18178
                     /gene="phoU"
                     /locus_tag="PB780_001186"
     CDS             17501..18178
                     /gene="phoU"
                     /locus_tag="PB780_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDE0733251.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            18415..19446
                     /locus_tag="PB780_001187"
     CDS             18415..19446
                     /locus_tag="PB780_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDE0733252.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTNQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKHGLFALGSLIINIVLLLLLLLIFTKIEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNLWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            19443..20210
                     /locus_tag="PB780_001188"
     CDS             19443..20210
                     /locus_tag="PB780_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDE0733253.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTTIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            20310..21878
                     /gene="liaX"
                     /locus_tag="PB780_001189"
     CDS             20310..21878
                     /gene="liaX"
                     /locus_tag="PB780_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HDE0733254.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            22175..22495
                     /locus_tag="PB780_001190"
     CDS             22175..22495
                     /locus_tag="PB780_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HDE0733255.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            22495..22845
                     /locus_tag="PB780_001191"
     CDS             22495..22845
                     /locus_tag="PB780_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDE0733256.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            23012..23950
                     /locus_tag="PB780_001192"
     CDS             23012..23950
                     /locus_tag="PB780_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDE0733257.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            23965..24795
                     /locus_tag="PB780_001193"
     CDS             23965..24795
                     /locus_tag="PB780_001193"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDE0733258.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            24839..25864
                     /locus_tag="PB780_001194"
     CDS             24839..25864
                     /locus_tag="PB780_001194"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDE0733259.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHCLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPVEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            25879..26817
                     /gene="galU"
                     /locus_tag="PB780_001195"
     CDS             25879..26817
                     /gene="galU"
                     /locus_tag="PB780_001195"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDE0733260.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            26942..27382
                     /locus_tag="PB780_001196"
     CDS             26942..27382
                     /locus_tag="PB780_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HDE0733261.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            27385..27987
                     /locus_tag="PB780_001197"
     CDS             27385..27987
                     /locus_tag="PB780_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDE0733262.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIIIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
BASE COUNT         9270 a         4813 c         6062 g         7862 t
ORIGIN      
        1 gttcgagagc tctttttttt gctttgctac cttgttcctt gaagattctg ctgatttaag
       61 atagagttaa gttaactaca agaattggag ggatccacat gaaatggaaa gaaaaagtag
      121 acgaagcagc tgaaaagtta tatgatttgg tcaaatcaga aaaatacaat attgaagtga
      181 atattccgaa aaaaggcgga aaagcggtcc gggtaaaatc aaaacgccca acgaatcata
      241 caaaaaaatg gatggctaaa aaccgctaat aataacaaaa gatccataag tcggcatttt
      301 catcgatttt agggtctttt gttattttct aggttggaca tatctaaaaa attgtttctg
      361 acaataggag taggaagaac gatgtgtgtc taaatcctct gttgatggat agaaaccttt
      421 atctttttca gttgtgaatc aggtatatac cagttataat agaagaaacg aaaggacggg
      481 tgtacgttat gtcgaatcaa ttacctgtat atatacagat tcatgaccaa ttaaaaaatg
      541 aaatcgaaca aggtatatgg aaagtcggag accgtcttcc ttcagaaaga gaattagcag
      601 tgaaattcaa tgtcagccgt atgacgttga gacaagcaat tcaaaacttg gcagatgaag
      661 gaatcttaga aagaaagatc ggttcgggta catatgttgc acgagaaaaa gtacaagaaa
      721 aaatgtctgg tgcaacaagt tttacagaaa ttatggaatc acaaaatcga gtaccgtcta
      781 gtcggacaat ctcttatttc ttgacttcgc caagctcaag tgaaatggaa aaactaaact
      841 tgagtaaaga tgacacgatc ttgcgaatgg aacgtattcg tttcgcagat gatgtcccta
      901 tttgttttga agttgccagt gtgccacaaa agctgatcga tggctacagc aaagcagaga
      961 ttactgaatc gttgtatcgc acgctagaag aaaaaggcgg gaaaaagatc ggtccagcga
     1021 atcaaacgat tactgctgtg ttggcttctg agaaaattgc tgactattta gaggtaaaac
     1081 gaggagatgc agttcttcgt ttgcgccaga tttcttattt tgaagacggt acgccgtttg
     1141 agtatgtgcg tacgcaatac gtaggaaatc gttttgagtt ttatttggaa aaataaaaaa
     1201 caggtgatcc ctgattccgt attagaaata aaagagaatc agaatcacct gtttttatat
     1261 atgatcagtt accagttgct cgtgataaga aacaggaccc tcttcaaatt ggacttgtcc
     1321 gttcagcagc tcaatgatgg attctttaaa gtggtcagca cttttttcat ctacagcaca
     1381 gatgacgcta acttgatcag tatattggat ttcttttata aaaaaatgtt ggagttcaag
     1441 ataattctgt aattttccta gttgcggata cgtgatcatc acagagatct cctgttgcaa
     1501 ggtaccttct acgattccaa tgtcagacaa tgcttgagag accgcatgag tgtatgctcg
     1561 tattaatccg cctgtgccga gtttcgttcc tccaaaatat cgagtgacta ctgcagctac
     1621 attgatcagt tgattttttt tgaggacttc caacatcggt atgcccgccg ttccacttgg
     1681 ttctccatca tcgctgcttc gctggatttc atttttttct ccaatgatga aggcgctgca
     1741 gttatggttc gctttccaat gttctttttt tatttcttga atgaaacttt ttgcttcgtc
     1801 ctctgattct attcgtctaa gagaacagat aaagcgtgat tttttgatct cgatctcact
     1861 ttgtccattt tcttttattg tacgatagcg ctcgatcatc ttgtcacctc ttttttcatt
     1921 ttctttattc agtataaata aagttctagg ttgtttcaac actgtaacgg ttgcgcaatt
     1981 gctgtagtat gcgataataa agacaaaggc aggtggatct ggggatgcag gaacgaatga
     2041 aaaagtacga tgcaattacg cattatttga agaacaatgg cggctctcaa gtaacgttga
     2101 cctttaccca atttgacgaa ctgctgtttc ccagtaacgg acttcccaaa acagcaaggg
     2161 aaagtacaga ttggtgggca aatgattata aacaccctga aaaaggtgcc tatggctgga
     2221 taaatgcagg ctatgaagta gttgtcatca atttggacaa agaatatgtc gtgttcaata
     2281 aattagtgaa atctagttgg ctatttgatt aaataaaccg cgtttagaaa acgttgacaa
     2341 atttctttct ttttatataa tagagtaaca gacgcgaaga aaacgtttgc gtagtttgtt
     2401 ttaaaaagga gagaaaagta tggctaaaaa aacaatcatg cttgtatgtt cggctggtat
     2461 gagcacaagt ttactagtaa caaaaatgca aaaagctgct gaagcaaaag gaatcgactc
     2521 tgatattttc gctgtgtccg cttctgacgc agataacaat cttgcaaata aagacgtgga
     2581 tgtgttgtta ttaggcccac aagttcgctt tatgaaagct gattttgaga aacgtctgga
     2641 accaaaaggc attcctttag atgtcatcaa tatggctgat tatggtatga tgaatggcga
     2701 aaaagtgtta gatcaagcaa tcacgctcat aggtgaataa atagaacagt ataactatcg
     2761 aacaagcgat ccaaaaaata gatcgcttgt tttttcgtat atttttttag ggcgaataat
     2821 atgaatcatt tttggggtag aaggcttctg acgagagata ttaaagcaat agactgtgag
     2881 gaggtaaatt taccaaatta tgagaagatc gcagcagtgg aacgtttgag caactggatt
     2941 ctttgtcatc gctgtggaat gagaacgtcc gtcattgatg gacagcttgc agattacggt
     3001 tatttttgta cacattgtct atatttaggg cgatgcgaca ctcaacaaga tttatatttg
     3061 tttgatcagc ctgaagcaaa acctcgagaa gtcgtctttt tttggacagg acagcttact
     3121 gaaaaacaaa cggaaatctc aaagagaata ctttgccatt cagaaaaaag acatcatctt
     3181 atttgggctg taacaggtgc agggaaaaca gagatgcttt atcctatttt agtggaaact
     3241 ttaaaagctg gaggaagagt agcgatttgt acaccgcgag tcgatgtttg taatgaattg
     3301 tttttacgct atcgttatgt ttttccagaa gaaaaaatca cgttgctcca tgggaataca
     3361 gcaacggctt atacctttac ttcttttgtt atctgtacga ttcatcagct ataccgtttt
     3421 tatcggtcct ttgatttaat agtgatcgat gagatcgatg cgtttcctta taacggggat
     3481 gcaggattag ctcatgctgt gcaaacagcg attaaaccag acggacgatt catctactta
     3541 agtgccacac ctgataaaaa gttactaaag gagatcaagc agacgtttga catccacaaa
     3601 cttgctttac gctttcatcg gcgtttgctt cctgaacccg tcttggtttt catgaataat
     3661 tggcgacaaa aatgcaagaa caagaagaag ataaaatctt tggtgcggat cattcatcaa
     3721 ttaattaaag aaaaccatat ccttttattt tgtccaagta tcacgatgat gcatcgtttg
     3781 aaagccacac ttcaagaggc tcttccgaga tatgagatca cagatgtttc ttcaagagac
     3841 aatcagcggg cggaaaaagt ccagaaaatg cgagaagcaa aatatgacat actgttaacc
     3901 acgatgatcc ttgaacgtgg agtcacattt gaaagaatat ccgttatcgt tttaggagcg
     3961 gatcatccgg tttttactaa atcttctttg gtacaaatcg caggaagagc cgatcgtaaa
     4021 gggccttata cgaacagtca agtatatttt ttctatgaag aaaaaactgc agctattcga
     4081 aaagcttgtc aggaaatcaa agaaatgaat gaagaaggga agaagatact gtgaggtgca
     4141 gttgctgcag taaagatatc gtccaaaatt taacattcag cgagattatt ggtgtttcga
     4201 aatacagatt acctcagact tgttcagttt gtcgcgcaca atttcattta ttggaaacaa
     4261 gaggctgttc tggctgtcaa aaaccaatcc atttcaatct agagagtacg aaaaagaaag
     4321 taaattctat ccccgtttac tgcagtgact gtcaagaatg gaagaagaaa tatccgacct
     4381 atgattttca gcaccgtgcc ttttttgtgt acgatgaaaa tatgaaggcc tggctcaatc
     4441 aatacaagtt tatgggagat atccgactag caggcacctt tgcatataaa tggacgcaga
     4501 taaaaaaaga atacccgaca tacatctatt gtccaatacc tttatctgaa aaaaggcgaa
     4561 cagaaagagg attcaatcaa gtaagcgaga tgttgaaagc agcaagaatt ccttttcaga
     4621 tgctactcaa aagagagaag catctgctgg ctcaagcaaa aaacacgcga aaggaaagat
     4681 tggagatgcc tcaacctttt gaacttgcag tcaccttttc taagatacaa aataaaaaaa
     4741 tattgattat tgatgatgtc tatacaacag gacagactct tttccacgca gcagattgtt
     4801 tgcagatcgc acagccgaaa atcatccgaa ctatttcttt ggcgagatga attagatgtg
     4861 gaaatttgat agcgatttaa tttgcaaata acctagagtt cgatataata gaattaagaa
     4921 gagggaaaga gtggtccttc tccttcttga ttgtggtaga cgaaaggggt aatttttatg
     4981 tttagatata atgtacgcgg cgaaaatatc gaagtaactg aagcgattcg cgactacgtt
     5041 gagaaaaaag taggcaaact agaacgctat ttcagtgacg cacccgatgc aacggcgcat
     5101 gtgaacttaa aagtttatac agaaaaaaca gcaaaagtgg aagtaacgat cccattacct
     5161 tatcttgtat tacgtgccga agaaacttct ccagatttat acgcaagtat cgatttggtt
     5221 gttgacaaat tggaacgcca aatccgcaaa ttcaaaacaa aaattaatcg taaatcccgt
     5281 gaaacgggca tgaatactgc aaatgctgct gctttattca atgaagagga cgaaaacgaa
     5341 aatgattctg aattagatat cgtacgtacg aaacgccttt cattgaaacc aatggatagt
     5401 gaagaagcag tcttgcaaat gaacatgctg ggacataact tctttatttt tgaagatgca
     5461 gaaacaaatg gtacaagtat cgtttaccgc cgtaaagatg gaaaatacgg actgattgaa
     5521 acagactgat cagccttact taaataacaa cgaaaacttg cagcagctac tgacagctgc
     5581 cgcaagtttt tttgagtttc ctcctctttt agatgaaata tgaataaatt atgaagttct
     5641 tactttaata cgcttaagat atttcaatta tctataacta atgataaaat gaaaagtaaa
     5701 gtctacatag tctggataat acacagaagg aaaaggagat tacacaacgc atggccaatt
     5761 tgattcgttc acttatcgaa aacgataaga aagaactaaa acgtttagat agcattgcaa
     5821 aaaaagttga agcatacgct gaccaaatgg cagcattaac tgatgaacaa ttacaggcaa
     5881 aaactgctga attcaagggc agataccaaa aaggagaaac attagatcaa ttacttccgg
     5941 aagcttttgc agtcgtccga gaagcagcaa aacgtgtatt aggtttatat ccataccacg
     6001 tacaattgat gggtggaatc gtgcttcacg atggaaatat ccctgaaatg cgtactggtg
     6061 aaggtaaaac cttgaccgca acgatgccag tatatctaaa tgctctgact ggcgaagggg
     6121 tacacgtagt taccgtcaat gaatatttag caacccgtga ctcgacggaa atgggcgaac
     6181 tttataattt cttaggatta acagtggggt tgaatatcaa ctcaaaatct tcggatgaaa
     6241 aacgcgaggc ttataattgt gatatcactt atagtacgaa taatgaatta ggatttgact
     6301 acttgagaga caatatggtc gtttaccgcc atcaaatggt acaacgtccg ctgaattatg
     6361 ccattgtcga tgaagttgac tcaatcttga ttgatgaagc gagaacgccg ctgatcattt
     6421 cagggcaagc agaaaaatcc acagctttat atacaagagc agacaacttc gtgaaacgtt
     6481 tgaaagaaga agaagattat aaaatcgatg tacaatcaaa aacaatcagt ttaaccgaag
     6541 cagggatcga aaaagcagaa gaaacattcg gtttggataa cttatacgac attgaaaata
     6601 ctgcattgac ccatcacttg gatcaagcat tgagagccaa ctatatcatg cttttagata
     6661 tcgattatgt tgtccaagac ggaaaagtac tgattgttga ccagttcact ggtcggatca
     6721 tggatggacg ccgttattca gatggacttc accaagcaat cgaagctaaa gaaggcgtag
     6781 agatcgaaga cgaaacgaaa accatggcga cgatcacttt ccagaactat ttccgtatgt
     6841 acaagaaact tgcgggaatg actggtacag ctaaaacaga agaagaagaa ttcagagaaa
     6901 tttataacat ccaagtatac cagattccga caaatcgtcc ggttatccgt gatgatcgtc
     6961 cagatttgct ttacccaaca ttacaaagca agttccacgc tgtcgtgcaa gatatcaaag
     7021 atcgctaccg caaaggtcaa cctgtgttag tcggaactgt tgcggttgaa acatctgagt
     7081 tattatcaga tatgctgaat caagaacgta ttcctcatga agtgctgaat gcgaaaaatc
     7141 actttaaaga agcagagatc atcatgaatg ctggtcaaaa aggtgctgtg acgattgcta
     7201 ccaacatggc tggtcgggga acagatatca agttaggact tggggtaaga gaacttggtg
     7261 gtctagcagt tatcgggaca gaacgacatg aatctcgtcg tatcgataac cagcttcgag
     7321 gacgtgcagg acgtcaagga gaccctggta tgtcacagtt ctacttgtca ttggaagatg
     7381 atttgatgaa acgattcggt tcagaacgaa tcaaagcttt cttagatcgg atgaagatcg
     7441 gtgaggaaga tgctgttatc caaagcaaga tgctgacgaa acaagtagaa tctgcacaaa
     7501 aacgtgtaga aggaaataac tatgacacac gtaaaaacgt cttacaatac gatgatgtga
     7561 tgcgtgaaca acgtgaagta atctataaac agcgacaaga agtaatcatg gaagaaaaaa
     7621 gcttatccaa gccactaatg aacatggtca aacggacaat cagtcgtgta gtagatgcgc
     7681 atacacaatt agagaaagaa aattggaatt tggaaagtat catcgatttt gccggcagta
     7741 ctcttgtcca tgaagatagt attactcttg cagatatcga aggaaaaaca gctcaggaga
     7801 tcaaagagta tctaaacgaa cgagcacaag aagtatttaa taccaaagcg gctcaactaa
     7861 atggtccaga gcaactgttg gaatttgaaa aagtcgtgat cttgcgtgtt gtggactcta
     7921 aatggacaga ccatattgac gcaatggatc aattacgtca gtcaatcggc ttacgtgctt
     7981 atggacagaa caatccatta gtcgaatatc aaacagaagg ctataagatg tttgaagata
     8041 tggtaggcgc catcgagtat gaagtcactc gaatctttat gaaagctgag atccgtcaaa
     8101 acgtacagcg tgaacaggtt tctcaaggaa cagcttctca ctcagaagac ggagatgcca
     8161 aagaaaatag taatactagc gcgaaaaaac agccaatcca tgtgaacaag gtcggaagaa
     8221 atgatccttg tccttgtgga agcgggaaaa aatataagaa ctgtcatgga aaaaatgcaa
     8281 actaatttct gacagcagga ttggtgagac aaattctttt tttaagaatt cgtctcacct
     8341 tctttctgtt atttgctgat tctgttagac tagatataat gaaaaaagta tagtgaaaga
     8401 aggaaaaaac atggaaatat ccgaaattcg aaatctcctg acagaaatgc aggaaaaggt
     8461 cacaagtttc aggaggtctc tttgacttag atcagctgga agaagacatc gcggaagcag
     8521 aagcaaaaat ggctgaacct ggtttttggg atgacagcga agccgcacaa aaagtgatca
     8581 gtgaaaataa cgctaataaa gaaacctatg accaattcca ttctcttgcc gaagaactag
     8641 aagaaatcga attgatggtg gagatgctgc aagaagaacc tgatgcagca atgcaagaag
     8701 aagcagaaac acgtatcaat gcattagatg aaaaaatgaa gacgtatgaa ttatctatgc
     8761 tgttagacgg accttatgat cgaaacaatg cgatcgtcga attgcatcca ggggctggcg
     8821 gaacggagtc ccaagactgg ggcagcatgc ttttacgtat gtatacacgt tgggcagagc
     8881 agcatggcta tcaagtagaa acactagatt atcaagcagg tgatgaagca gggatcaaga
     8941 gtgtgaccct gctgattaaa ggacataacg cttttggtta tctaaaatca gaaaaaggtg
     9001 ttcatcgcct tgtacggatt tcgccttttg actcagcgaa acgacgtcat acttcttttt
     9061 gttcggtaga tgtgatgcca gagcttgatg aaacgatcga tatagaaatc aatccagatg
     9121 atttgaaaat cgatacgtac cgagcaagtg gtgcaggtgg tcagcacatc aataaaacag
     9181 aatctgctgt acggatcact cacttgccta caggaacagt agtagctagt caggcacaac
     9241 gctcacaatt gaaaaaccgt gagcaagcga tggggatgtt aaaagctaag ctttatcaat
     9301 tagaagtgga aaagaaagaa caagaagcag ctgcacttcg aggagaacaa aaagaaatcg
     9361 gctggggttc acagatccgt tcttatgttt tccatcccta ttctatggta aaagaccatc
     9421 ggacaaacgt agagacagga aatgtccaag cagttatgga cggtgacctt gatttattta
     9481 ttgacggcta tttaaaaatg catctacaat aagaacccat tgttaaattg aaataaaatt
     9541 gtaagatttt gcaagcatgt tgaaacattg ccatgctata atagctaaag actgaaaaag
     9601 atatggagag aacgccatga ttgaaatgaa ggatgtaatg aagaagtact caaatggtac
     9661 gaccgctatc cgcaatctct cgattgtcat cgaacaagga gagtttgtct atgtggttgg
     9721 tccgtctgga gccggtaaat ctacattcat caaattgatg tatcgagaag aaaaagcttc
     9781 aaaaggaact ttgaacgtcg caggtcatga tctaatagca atcaaaaacc gcgaagttcc
     9841 ttatctacgc agagaaatcg gcgtggtgtt ccaagactat aaattattac caagaaaaac
     9901 agtatatgaa aatgttgcgt acgctatgca agttatcggc agaaaacctc gagatatcaa
     9961 aaaacgagta atggaagtgc tggaccttgt tggtttgaaa cacaaagttc gagtctttcc
    10021 tagcgaatta tccggtgggg aacagcaacg tgtctctatt gctcgggcaa tcgtgaatac
    10081 gcctaaagta cttatcgcgg atgaaccgac aggaaatctg gatccagaaa actcatggga
    10141 aatcatgaaa ttattagatc ggatcaatgc ccaaggaact actgtcgtga tggctacaca
    10201 taacagtacg atcgtaaata cgattcggca ccgagtaatt gctatcgaaa acggccgcat
    10261 tattcgagat caagcggaag gggaatatgg atacgatgat tagaaccttt ttccgccatt
    10321 tattagaaag tatcaagagt ttgaaacgta acggctggat gacagttgct tcagtcagtg
    10381 cggtaacgat cacgttgact ttagtcggtg tattcatggc agttatcatg aatgcaacta
    10441 aattagctca ggatatagaa ggaaacgttg atgtatctgt attcgtagat atcggtacaa
    10501 aagacgaaga gatgaaaaag ctagaaaaag aattgaatga cttagatcat gttaaaaaag
    10561 ttgaattttc aagtaaagaa caggaattga aaaaaatcca aaacgaaatg ggtgattcct
    10621 ggaaattgtt tgaaggtgac aataacccgt tatatgatgt gtatgtagta agtgctactg
    10681 atccttctta tacaaaacaa gtagcagaag aagcagcaga tttgaagaat gtttctcgtt
    10741 ctgattacgg tggagcttcc tcagatcgga ttttccaaat cgctggtgca gttcggacat
    10801 ggggattagt tgcagcggca ttgctgctgt tcgtagcgat gtttctcatt tcgaacacga
    10861 ttcggatcac gattttgtct agacagcgtg aaattcaaat catgcgttta gtcggtgcaa
    10921 aaaatggata tattcgctgg ccgtttttcc ttgaaggtgg ctggatcggt ctgatcggtg
    10981 cgattcttcc gattttgatc atcacatttg gttatgcatg gtttttccaa ttgatcaatc
    11041 catcattatt acgttcccat tattcattga ttcatccaca aaatatcgtt tggaaaatca
    11101 atcttttaat ggtcggcatt ggtgtgatca ttggttcact tggctcgatc atttctatga
    11161 gacgtttctt gaaattctaa taccacaata taaataaaat gagaaagttg gacagtttcg
    11221 ttccagcttt ctttttttat tggctaggag aatagaagga agcggctttt ttccctttac
    11281 tttaaaaaac aacttgggta ttcttaagtt ggtagaggaa gaggtgaagc cgtgaaaatt
    11341 atcgttgaca atttttttac gatccttttg attatgaata ttttgctttc ctttatcatc
    11401 gtttttcgag aaagaaagga aactgctcaa acatgggcat ggctgctcgt tttgatgttc
    11461 attccagttg taggatttat tttatatatt tttttaggaa gagggatttc caaagagaaa
    11521 atctttgatt tgaagatcca agggaaaatc ggtaagaacc tggaaattga agaagacaaa
    11581 caggcattga tgcgagggct ttatcctcat ccgcctactg gacaagtaga tgtgaaacaa
    11641 ttgatctata tgctcacggt atttgaaagt acgctctata cgactaaaaa tgaaatcact
    11701 ttatttacag atggtcgtga aaaattcgat gcgctgattg aagatatcaa gcaagcagaa
    11761 gaccatatcc acttccagta ttatatttat cgcagtgatg cattaggtga agaagttcga
    11821 gatgcattga cagaggcggc aagacgaggg gtaaaagtca gagtccttct tgatgcatgg
    11881 ggttccacac aagtttcttc cagctttttt cagaatttga aaaaagcagg cggagaaatc
    11941 gcatttttct ttcctctatt tgttccgtat attaatcctc ggatcaatta tcgcaatcat
    12001 cgtaaaatcg tagttattga tgggaaaatc ggatatacag gtgggttcaa tgttggaaat
    12061 gaatatctag gattggtaaa gaaatttggt tattggcgag acaatcattt gcgtatttac
    12121 ggagaagcgg tgtatatctt gcaaaaccgt tttttgatgg actggaattc tcagcataca
    12181 aaagaagtag gctattctcc caaatttttt ccgtccattc actctactgg tgatatagct
    12241 gttcaaatcg tgacaagcgg accagatacc gaacatgaac aaatcaaaat gacttatttg
    12301 aaaatgattt cgttagccaa acgggagata ttgatccaaa cgccttatta cataccggat
    12361 ggatcgattc atgaagcact gaagttggct cttctttcag gcgtaaaagt tcatatccaa
    12421 atacctaaca aaccagatca tcttctcgtt tactgggcaa cgtattcttt cgctgcagag
    12481 ctgatcgagt atggagcaag gatcgaaaca tatgagaatg gatttattca tgcaaagacg
    12541 atgattatcg atggtggcat tgtttcagtt ggctcagcaa atatcgatgt tcgttcattt
    12601 cgcttggact ttgaggtcaa tacactgatt tatgatgaac gaatggcttc acgtgtaaga
    12661 aaagcatttt ttgaagactc gaaaatttct acgcatctca caaaagaaat gtatgaaaac
    12721 cgaggaataa tcatcaagat gaaagaagga ttagcaagat tgatttcgcc gctcctttaa
    12781 aacacaaaaa ggacatgaga accatcaaca gatggagatc tcgtgtcctt ttctaacggg
    12841 gccatttctg agagtacgtc agaaatggag aaagcgtctc gcatgagcga aattaacttt
    12901 ggggtatctc ttgttttttg gggaaagcaa gaaataagct aatatatgct attaaaagag
    12961 acacatttca aagccagtat agcatagaaa cttttaatga gatagttttt tttacaatta
    13021 tgaggattta ttttgttatt taatgatttt gattcgtttt catcaagtaa tgaaaatgat
    13081 aaaataaaca tactttagga ttaaaggaga tttctaatga aaaaacttaa attattctta
    13141 ccaataatag gattagctat tcttttatca ggctgttcaa agtggattga tcgaggagaa
    13201 tccattacgg ctgttggctc ttctgcttta cagcctttag tagaaacagt tgctgaaact
    13261 taccagacag aaaatcctgg aaaattcatc aatgtacaag ggggcggaag cggaactggt
    13321 ttgtctcaag tacagtcagg agcagtcgac atcggaaact ctgatttgtt tgcggaagaa
    13381 aaatcgggga tcgatgcctc agcattagtc gatcatcgag tagcagttgt tgggatcact
    13441 cctattgtga acaaggatat tggcgtaaaa gacatcagta tggaaaacct gaaaaaacta
    13501 tttacaggcg agatcaaaaa ctggagtgaa gtcggtggaa aagatcagcc agtagttata
    13561 ttgaaccgtg cttcaggaag cggaacccgt agcacgtttg aaaaatgggt attggacgga
    13621 gaaacagctg ttcaggcaca agaacaagat tcaagcggaa tggtgcgcca gatcgttgcc
    13681 gacacacccg gtgcaatcag ctatgttgct ttttcttatg tttccgatga tgtagatacg
    13741 ttgaatgtta acggtgtaga accgacagat gaaaatgtga caacgaacaa atggacgatt
    13801 tgggcatatg agcatatgta cacaagagat catccaaaaa aactgacaaa agaattttta
    13861 gactatatct tatctgatga agtacaagat aacatcgttg gagaactagg ttatatccct
    13921 gtttctcata tgaaagtcga acgtgattgg caaggaaata tcgttaaaga gtaatcttta
    13981 cacaaaattt acaaacttag ctaaactttt acatgttatt aaaaccaaat tgatgttcaa
    14041 tttgtacact gagtgagtat gttaaacctg attaaggggg agtcatctgt ggaagatgtg
    14101 aaaaaagtat tattaacaaa atcaaaacgt tcaaaaatgg aacagcgtgg aaaattcatc
    14161 agttttttat gtatcgcgct gatcgtgttg gttgtattat ctatttttta ttttgtagct
    14221 agtaaaggat tggccacctt ttttactgat aagatcaatg tatttgattt tctgtttggt
    14281 acagattgga atccaagcca aatcggagca aatgggaaac caatggtagg ggcattgcct
    14341 atgatcacag gttcgtttat tgtgaccttt ttatctgcta ttgttgccac accatttgcc
    14401 attggtgcag cagtattcat gacggagatc tctcctaaaa acggaacgaa gatcttacag
    14461 ccggtcatcg agctgcttgt aggtattcct tctgtcgttt atggttttat cggtttatca
    14521 gttatcgtgc cttttgtcag aagtattttt ggcgggacag ggtttggtat cttagctggt
    14581 acatttgtgt tgttcgttat gatcttgccg actgtcacaa cgatgactgt tgatgcatta
    14641 aaggcagtac cacggcacta tcgggaagct tcattagcgc ttggtgccac aagatggcaa
    14701 acaatctaca aagtcgtgct acgagcagcc gtgcccggga ttttaacagc agtcgttttt
    14761 ggtatggctc gtgcgtttgg tgaagcctta gctatccaaa tggtcatcgg taatgcagca
    14821 ttgatgccaa caggacttac tactcctgct tctacattga cgtccatctt aacaatggga
    14881 atcggaaata caatcatggg aacagttgaa aataatgtgt tatggtcatt agcgctgatc
    14941 ttgctattga tgtcattatt ctttaatatc gtgatccgta tgattgggaa gaaaggagag
    15001 ctgaaataat gaacgcaaaa cgctcagata aaattgctac aggtgtttta tatgtagttt
    15061 cggggatcat cgtattgatt ttagcagctt tactacttta tatcttagtt cggggaatac
    15121 cgcacatttc atggtccttc ttgaccgaac cttccaaagc ctaccaagaa ggcggtggaa
    15181 tcgggatcca gctatttaac tcgatttatt tgcttttgat cacgatgatc atcagttttc
    15241 ctatttcttt aggtgcgggt atctatcttt cagaatatgc tggaaaaaac tggctgacag
    15301 atgtgattcg cacgtcgatt gaaatcttga gctcattgcc ttctgtcgtt gtcggtttat
    15361 tcggtttctt ggttttcgtt attcaattcc agtatggttt ctcgattatt tctggtgcat
    15421 tggctttgac tgtattcaac ttgcctcttt tgactagaac agttgaggag tctttacaag
    15481 cggtccatta cacacaacga gaagcaggat tggctttagg tttgtcacgc tgggaaacag
    15541 tgataaaagt ggtggttcca gaagcgacac cagggatttt gactggtgtg atcctaagtt
    15601 ctggaagaat ttttggtgaa gcagctgcat tgatctatac ggctggacaa agtgcacctg
    15661 cgttagattt tacgaactgg aatccattaa gtgtttcaag cccgatcagc attttccgtc
    15721 aagctgaaac attagctgtc catatttgga agatcaatac agaaggcacg atgcctgatg
    15781 gtgtttctgt ttcggctggc gcttctgccg tgttgatcat tgctgtttta ctgttcaact
    15841 tcggcgctcg tgcgattggc aaacgattat accgaaaaat gacttcagcc taaggagaag
    15901 aagcaaaatg aaaaaatata actggaatga aacaaatgtc atcaccatgc caaaagagaa
    15961 agaagtagca ctatatacgg atgatctaca tgtctggtat ggcgacaatg aagcaatcaa
    16021 aggagtggat ctggaatttg aaaaaaataa gatcactgca ctgattggtc cttccggctg
    16081 cggaaaatct acgtatcttc gttcgttgaa tcgaatgaat gatgggatcg ctaatacgaa
    16141 aatcactggg aaaatcatgt atcaaggcgt agatattaat gcgcctcaag tggatgttta
    16201 tgagatgcgc aaacggattg gcatggtttt tcaacgtcct aatccgttca gcaagtcgat
    16261 ttatgacaat attacatttg ctttgaaaca gcacggcgaa aaaaacaaaa agaaattaga
    16321 tgaaatcgtt gaaacaagct tgaaacaagc tgccttgtgg gaccaagtaa aagacagctt
    16381 ggacaaaagt gccttggctt tatctggcgg gcaacagcaa cgtttatgta ttgctcgtgc
    16441 gatcgcgatg aaacctgata tcttgctgct agacgagccg gcaagtgctt tggaccctat
    16501 ttcgacagga acggttgaag aaacacttgt aaacttgaaa gaagactata cgatcatcat
    16561 cgtgacgcat aacatgcaac aagcggctcg tatcagcgac tatactgcct ttttctatct
    16621 aggaaaagta atggaatatg acaaaactaa caagatattt accagaccaa aaattcaggc
    16681 aaccgaagat tatgtgtccg gtcattttgg ttaagacgga ggcctcaaca tgacaaaaga
    16741 aattatttca tctaaagatc ttcatctgta ttatggaaag aaagaagcac taaaagggat
    16801 cgatctaagt ttcaatcaag gggaaatcac tgcgatgatc ggtccttctg gttgcgggaa
    16861 atccacttat ctgcgttctt tgaaccggat gaatgacctg attccgaatg taacgatcac
    16921 cgggagtgtt ttatataaag acaaagacat ctatggtcca aaaacagatg ttgttgaact
    16981 aagaaaagaa atcggcatgg tattccagca gccaaaccca tttccttttt ctatctatga
    17041 aaatgtcatc tatggattga aattaaaagg ggaaaaagat aaaaaagtcc ttgatcaagt
    17101 agttgaagaa agcctgaagg cagcgtctgt ttgggatgat gtgaaagaca aattgcataa
    17161 aagtgcttta tctttatccg gtgggcagca gcagcgtgta tgtatcgcac gtgttttggc
    17221 agttaatccg gaaatcatct tattggatga accaacaagt gcgcttgatc ctgtttctac
    17281 tgggaaaatc gaaagtatgc tgttagaatt aagagagcgg tatacgatga tcatcgtgac
    17341 ccataatatg tctcaagctt cacgtatttc agataagacg gcattctttt tagatggtca
    17401 cttgatcgaa tacaatgata cgaagaagat attcatgaat ccagaaaaac aagagacaga
    17461 agattatatt tcaggtcgat ttggataagg aggaaacgga atgctacgta cacaatttga
    17521 agaagaattg ctgaatctcc ataatcagtt ctacgaaatg gggatgatgg tgagcagcgc
    17581 cgtgcacaag tcagttcgtg catacatcaa acacgataaa atcttagccc aagaagttat
    17641 cgaaaacgat ataaatatca ataacatgga aacacgcttg gagaagaaaa gttttgaaat
    17701 gatcgcattg caacagccag ttacaactga tttgcgtatg atcattacgg tcatgaaagc
    17761 tagctcggat ttagaacgta tggccgatca tgcggtgtct gttgctaaat ccacgattcg
    17821 cgtaaaaggg cagacgagaa taccggaaat cgaaaaagag atctccgata tgtccgatta
    17881 tgtgaaaaaa atggtggaca acgtcctagt tgcttatgtc aaaacagacg aaaaagacgc
    17941 acgtacgatc gccaatatgg atcaaagagt caatgactat ttcaatcgaa tctataatgc
    18001 aacaatcaaa aatatgcagg aaaatcctga gacagtcatc agtggaacag actacttgaa
    18061 tgttgccagt tatctagaac ggatcggaga ctatgtgaca aacatctgtg aatggatcgt
    18121 ctacttggct acagggaaaa tcactgagtt aaatacgaac cacaatgaga ttttctaaac
    18181 taaagcggtg attctgccgt ttgtttagga aaagaggttg tgaaatcagc ggttgtgaaa
    18241 aagctgttct gcatcaagta ataagaacag tcaaacaaaa atagctcctc atatttttca
    18301 tttggctgga cttattaccg cagatgcaag cttttgaaca ctgtttattc gggaaagggt
    18361 ttgtgacaaa atatgtcaca aacccttttc atcatttcat agaaaggata aagaatgcaa
    18421 aaaaaaatta gcatcatcac tatatttttt atttgtgtgc ttacttcatg ggttattcag
    18481 catcaagctg ctttatatga agtacctgtc atcgagatcg aaaaagtacg aataaaagag
    18541 gatcatcaat tgattacagg aagactgatg aacacgaatc aaaaaggaga atcgatttat
    18601 ttatcggttc cttatcaaaa aaatcaaata gaaacagcaa atataaaagc gagacaaatg
    18661 tatctagtcc aaatgcatgg agatgaatgg cagctagtag caaaaaaaaa tgatggctgg
    18721 cttttcttct tcacaagtat cttcattggc gccatgttgc ttattggcgg aaaacatggt
    18781 ctatttgctt taggaagtct catcatcaat atcgttttac ttttactttt gttgctcatc
    18841 tttactaaaa tagaagggat ttctctatta aatatatcta tcctgttcgt cgttataggg
    18901 atgattatta gcatattggc tttagatgga tggaatagaa gcagtataat caaaatttca
    18961 gctgcagtta cgagtgtttt tttagcattt tttatttgtc ttttgacaat gaatctttgg
    19021 aaagaccaag gactgcgcta tgaggaactg aattatctga cacgaccgta tcgttctgtt
    19081 tttctttcta gcgtactgat tggaacagct ggaggaacac tcgataccgt tatcacagtc
    19141 attgcgacat tagaagaatt gactaagcga caaccagaaa taacagcgaa acagttatgg
    19201 ctttcgggaa ggcaagtggg tcgagatgtg attggctcga tgaccaatat ccttttgttc
    19261 gtctatatca gtgggtcagt tccatcgctt cttttatatt taggaaatca atggtcgttc
    19321 aaagaaacga tagaacagca tttatcattg gaatttttac gcgtcattgc tggcagctta
    19381 ggaatagtat tatcgatccc tatctcggtc ctcttttttc tgactgtaag gaggctgaaa
    19441 cgatgaatgt acttattata cttgttggga ttttagcaat cagcgtgcta cttattggcg
    19501 gtacgcaagg tatgtatatt cttttaggct tattcatcaa tttagggatc tttttcttgt
    19561 tgttattcgg ttatcatcag aagtggccga tactggcatt gtcggtcata ggatttttat
    19621 tgattgctgt ggttatctta ttttttatca acggttataa tctaaaaatg cgtgcagctt
    19681 ttgtgagtat actgatcttt ttattttgtt tcctactatt gcttcctatc actgattttt
    19741 tagcaatcca aggttttaca acgatagaac tagaagaatt atctggctta gataagactt
    19801 tagcgattga ttttagatta cttacgagat cgttgcttct gattagtctt agtggtgctg
    19861 ttttagatgc gagtgtagca atttctagtg ggacattcga agtatatcag gcaaatccgc
    19921 aattatcttt ttaccaattg cgccattcta gtttcgctat tgctaaaaaa atattggcgt
    19981 caacagtaat gacacttctt tttgcattca tgggaagttc tttagcgttg atcctctggt
    20041 ttattgattt agacattcct tttcagcaag tgatcaatga aaaatcattt gttctagaat
    20101 atacgatggc tattcttacc acattgtccg cactacttgt tcttccttta acctgcgtga
    20161 ctacagcata tctctttaca aaaaagaaag aacaactcaa gatggagtaa aagttacaac
    20221 ttaagtccta tgacattttt actgaatcag tgcataatat ggatgtaagg aaaagcttgc
    20281 aaacaatttt gcttaaggag gcaatttcca tgaaagaaag agaacgtatt ttagatttag
    20341 tgaaaaaagg aatcctttcg actgaagaag gcttagattt attagaaagc atggcaactg
    20401 aaaaagacga aaagcaaatc aaaaaagaag cagacaaggt aactgctagt cataaagaaa
    20461 aagatcaggc aagtcaattg atcgataaac ttgaaaacgg cgaagaagaa atttctgagc
    20521 cagttgatcc aaaggaaaga gaaagacaag atcaagaaaa cttagaaaag atcttagatg
    20581 aactagcaac tgaagcgaat aaaacttcgg cgcgtctaga tgaagtaaat gctcaattcg
    20641 ctgacaataa ggcagcacgc aatgagaaac aggaagcatt gatgcagctg aatacaaaag
    20701 aagaattagg cgagttgaca gaagaagaat tagcacagcg tcagacgtta gaagcagaaa
    20761 tcaaagagtt agaagctagt ggcgatacac tgttagaaga acaattgaaa ttagaagcag
    20821 aattaaaaga tatccgtaag aatcaatggt ctgagaaaaa agaaacgttt actgataagt
    20881 ttgaattgcc agatgattgg aaagatcaag caacagatac actgaatcaa gtaggcgaaa
    20941 aaatgtcaga agctggatca cagttaggaa aattcttgaa gaaaacattc caaactgttt
    21001 cagaaacagt gaatgacaac atggaatgga aagatgtcag cttgcgtgtc cctgggattg
    21061 cgacaacgaa atttgagcat gaattttact atgaagctcc tgcagcaagt atccttgata
    21121 tcaaggcagc aaacggaaac gtgacgttaa aaacgtggga cagcaacgat gtgaaagttg
    21181 aagctaagat caaactttac ggaaaaatgg gtgctgaacc atttgaagca ttctctgaac
    21241 ggagccagat tgaggttaat gaagatcata tctccttcca aattccaaat aaacgtgtgc
    21301 gtgcagacct tgttttctac ttacctaaac gagtatacga ccacgcagct attaaacttc
    21361 taaatggcaa catcatgatt gaaacattgg aagctaaaga tatttataca aaatctacga
    21421 atggtaacat catcgtggac caattaactg caacgatgct ggaagtagaa ggtgtaaacg
    21481 gcaatatcga tattcgaaac gggaacatct tagattctat tatcgaaaca gtaaatggta
    21541 cagtgacatt tggtgcgaca gcagaaaacc tttctgtgtc tttggtaaat ggtgatgtac
    21601 gtcttacgat taaagaagat aatttgaaaa aagtagaagc aagttcagtg aacggaaatg
    21661 tcaaagtcgc tttaccagat ggcatcggtt tggaaggaca tgctaaaaca agtcttggaa
    21721 gtatcaacag ccgtctgtcc aattacgaag tagttcgtga gaaaaaagaa cggacaaacc
    21781 aaatgttgca attccgtcgt gtttcagatg gtgaaatcgc gcaagtgcaa ctttcaacga
    21841 caacaggaag tatctattta aaagatacag ataaataaag aggttgtgaa agagatgttc
    21901 agccttgagc aataagaagc gattttgtaa aatagcttcc cctattttac aaaattgtgc
    21961 cttattgccg ataggctatc tcttgaacac cgtttaccaa gagattatga acttgccaat
    22021 tatcgccgag tcacaaggaa taatttcaaa aaaagctcgt cacgttttat gaatatggta
    22081 atttgtgacc gagaattttg caaaaagaca ccgattaaga agaaggttgt cacaacgatt
    22141 ttcttcattt aaaatagtag aacagaggtg agacatgatg aaaaaaaagt tgacaaaatc
    22201 caataaaaat gttgttttga cagggacttt agcaggaata ggagaataca tcgggattga
    22261 tccgactgtt atccgtgtgc tttatgtatt cctgagctta gttgcttttg gaagtccaat
    22321 cattttatac attttattag ctttgattat cccatctgcg cgttctgcac gaacaaacta
    22381 tggacataac aatgcatatt atgaaaaaaa caattatcgt gaacaaactc gtgaacaaac
    22441 aaaaaaacgc aaagaagcgg aaaaaatcga tgatgacgaa tggagtgact tttaatgtcg
    22501 tactttcaac gtattttagt taatacgtta acattcatat cattggctgt tctttttcct
    22561 gcgatggtcc atgtgagcag tatttggacg gccgttcttg ccagcttcgt attgtctgtg
    22621 ttgaatatgt ttgtcaaacc gattttgacg atactgtccc tgccctttac attagtcaca
    22681 ttgggattgt ttacgtttgt tatcaatgca gcgattctta aaatgacctc cttttttgtg
    22741 ggagcagaac attttagttt ttcttcattc ggtgcagcgt tgttagtagc tatcttgatg
    22801 tcgcttgtca atgtaattgt cagcgaacat aacagtagaa aataaaagaa agctgtgccg
    22861 aactatcggc gcagtttttt tatttagaat caggaatgcg ttttctatta cattggtaat
    22921 taaataaaaa cgtttttttt aattggtagt acttttggct agacaatgat aaaatgatag
    22981 agaacactct aatagaaatg aggtagcatg aatgtcagaa gttgtaaaag ttagccaatt
    23041 agtgaaaaaa ttatctttgg aaatcgtgac aggcgacgag aaaagcttag accgtgtcat
    23101 cgtaactggg gacatctcac gcccaggact agagttgact ggttatttta attattattc
    23161 ccataatcgt ttacagctat ttggcagtaa agaaatcagt tttgcggaac ggatgatccc
    23221 agaagagcgt ttgatcatca tgagaagatt atgtagtgaa gatatgcccg catttattat
    23281 ttcaagagga ttaccagctc cagaagaaat gatccaggca gcaaatgaaa atgggatagc
    23341 tgtactgcgt tctcccatct caacttctcg cttattagga gaattatcca gttatttaga
    23401 tggtcgcctt gcccctcgaa caagtgtcca cggtgtttta gtggatgttt acggattagg
    23461 tgtactgatt caaggtgaca gtgggatagg aaaaagtgaa acagcgttgg agttgatcaa
    23521 acgcggtcat cgattgatcg cagacgatcg agtagatgtt tatcagcaag atgaattaac
    23581 ggtgatcgga gaaccgccta aaatcctgga acatctgatt gaaatccgag gaatcgggat
    23641 cattgatgta atgaatcttt ttggtgcgag tgcggtacgt ggctctatgc aagtccagtt
    23701 ggctgtttat ctggaagcat gggcaaaaga taagaaatac gaccgtctgg gctctgaaga
    23761 cacgaccgtt gaaatcgcgg aagtaggcat tccacaaata aaaatccctg tcaaaacagg
    23821 acgaaacgtt gcaatcatca ttgaagtagc cgcgatgaat ttccgtgcac gtacaatggg
    23881 ctttgacgca acgaaaacat ttgaagaaag attaagtcgc ttgattgaag aaaactcagg
    23941 caacgactag caaggagtga caatatgtta gcacaaatca atcgagttgc ttttgagctt
    24001 gtagggattc ctatctactg gtatgcattg atcatcgtat caggtatcat tatcgccatg
    24061 tggcttagca gcagagaggc tgttcgggta ggattaaaag cagatgatgt gacagatttt
    24121 atgttgtggg gattgcctat ctccattatt ggcgctcgct tatattatgt tttatttgat
    24181 cttccccagt atatagctga tccaatccaa atattcaata tccgttcagg aggcttagct
    24241 atttatggcg gactgattgc aggcgcgatc acgctgtatt tttttactaa atatcatttt
    24301 atttctttgt ggactttttt agatatcgct gcaccaagtg tattattagc acaagcaatc
    24361 ggtcgttggg gaaatttcat gaaccacgaa gcatatggac cagacacgac gcgaagcttc
    24421 ttagaaagtc tgcatttacc gcaattcatc attgataata tgtatatcga agggatctat
    24481 cgtcaaccaa catttcttta tgaatcactt tggagtttga ttggatttat tttgcttttg
    24541 tttttacgaa agaaaccaca ccttttgaaa aaaggtgaag tccttttatg ttatctaatg
    24601 tggtatagtt tcgggcgttt ctttattgaa ggattacgta cagacagtct ttacttttta
    24661 ggtgtgatcc gtatttctca attattatct gctgtgttat ttgtcggtac attgattcta
    24721 tttatttggc gcagaaaaaa aggaaattta ccggattatg accgctcatt aggaaaaaat
    24781 caacaaatta tctgattttt agagcgttaa aaatgaaaaa aatttcagga gtggtaaaat
    24841 gaattataga gtagcggttt taggaccagg ctcttggggt actgctttgg cacaagtcct
    24901 cgccgaaaac ggacatgacg ttcggatttg gggacatcgt gcttcacaag tagaagaaat
    24961 caacacccaa cacacgaatc aacgttattt accagatcat tgtctacccg attccatcca
    25021 agcattcacc aatatggaag atgcagtaaa agacgcagat gcggtgctat tcgtcattcc
    25081 aacgaaagca atacgttctg ttgcaaaaga actagtgcct aaattgaata caaagccagt
    25141 gatcatccat gcaagtaaag gattagaaca ggggacacat aaaaggatct ctgacatctt
    25201 gttagaggaa atcccagtag aaaaaagaaa agctgttgtt gttctttctg gaccaagcca
    25261 tgcagaagaa gtagcgaaac atgacatcac tacgatcaca gctgcaagta cagatgcaaa
    25321 agcagcaaaa tatgtacaag agctgttcat gaataattat tttcggatct ataccaatcc
    25381 tgatgtgatc ggtgtagaga tgggggcagc tttgaaaaat attattgcta ttggtgctgg
    25441 cgctattcac ggcttagggt ttggcgataa tgcaaaggca gcaatcatga cgcgcggatt
    25501 agctgagatc agccgtttag gtgtagcaat gggtgccaac ccgctgacct ttatcggatt
    25561 aagcggagta ggcgatctag tcgttacttg cacaagtgtt cattctagga actggcgcgc
    25621 aggcaacttg ttaggacaag ggcagcccct agaagaggtg ctagccaata tgggaatggt
    25681 cgttgaaggt gtgaatacta cccaagcagc gatggaacta gcccaaataa tggaaatcga
    25741 aatgcccatc actcaaacaa tctatgaagt gctgtatgaa caaaaagaaa tcaaaacagc
    25801 cgcaaaagaa atcatgttac gtgatgggaa aaaagaaaat gaattctcat taacaatttt
    25861 ttgaaggagc gaatgtatat gaaagtcaaa aaagcagtta ttccagcagc aggattagga
    25921 acaagatttt taccagcaac aaaagctatg gcaaaggaaa tgttgccaat tgttgataaa
    25981 ccaacaatcc agttcatcgt tgaagaagcg ttgaaatcag gtatcgaaga catcttgatc
    26041 gttacaggaa aagctaaacg accaatcgaa gatcactttg attcaaatat tgaattagaa
    26101 acaaacctaa gtgaaaaagg aaagaccgaa ttattgaaat tagtagaaga aacgacagat
    26161 gtcaacttgc atttcatccg tcaatctcat ccaaagggtt taggacatgc cgttcttcaa
    26221 gcaagagcct ttattggaaa tgaaccattt gtcgttatgc taggcgatga tataatggaa
    26281 gacgaagtac cattgacaca gcaattaatc gatgattacg aacgtactca tgcttctacg
    26341 atcgccgtta tgcaagttcc tcatgatgaa acttcaaaat acggaattat caatccagga
    26401 gaagtattgg aagatgggtt atacaatgtc aaaaactttg ttgaaaaacc agatccaagc
    26461 aaagcaccaa gtgatttagc tattattgga cgttacttat tgactccaga aatttttgat
    26521 gtattgtccg agcaacaacc aggagccgga aatgaaatcc aactaactga cgcaatcgat
    26581 acattgaata aaacacaacg cgtatttgcc cgtaaattca caggtaaacg ttacgatgtc
    26641 ggtgacaaat ttggttttat gaaaacaagt attgaatatg gtctaacaca tccagaagta
    26701 ggtgcacctt tacgtgagta catcctgtct ttaggtaaaa cattagaagc agaaggaagc
    26761 aagatcgatc ataatccaaa aggaataaca gaaatcaaag aagaaaagaa aaaataaccc
    26821 ttttttctaa cgagcttcag ctgctaattg aataaaaagt agttgaagct cgtttttatt
    26881 tgtttaaaaa acacgaaaat gctatcctta tagaaaagag agacaagaag ggagtttgat
    26941 catgtcagga ggagaaattg caggattgat cgcagcaatc gcctttgcga tacttgtcgt
    27001 ttttatcgtt cgtgtgttgt tgcaagtttc taaaacttta gaaaaagtag atcagactgt
    27061 agcagaagca aatacgacga tcgaaatcgt cacaaaagac gtagatcttt tgtctagaca
    27121 agtagaaggc ttactggtca aaagtaatga actgctcttg gatatcaatg gaaaagtcgc
    27181 aacgatcgat ccattgttca cagcagttgc tgatttaagt gaaagtgtat cagaacttaa
    27241 tcattctagt aaaaacatcg cgacgaaagt cggcggaatc ggtaaaacga cagctaaagc
    27301 gacggttgcc ggaaaagtcg gtgggacagc gatgaaattt tttaaaaaca aaaaacatac
    27361 tgaaacgaca ggaggaaaat aaaaatggct aaaaaaggtg gatttttatt aggtgcagta
    27421 gtcggaggca cagccgcagc aatcgccgcg ttattgttag caccaaaatc tggtaaagaa
    27481 cttcgggatg aattagctgc ccaagcggat gatttgatgg atatggcttc agattacaca
    27541 gatatggcga aagaaaaatc gactgagtta acaggtattg caaaagaaaa agcgcaagat
    27601 ctttctcaac aagcaggtga tctagcaggt agtgtcaaac aaaaagtaag tgaatcagca
    27661 gatgaagcag gaagtgaatc tgacgatatc ttgttaaatc ttaaagaaca atcagctgac
    27721 ttatctaatc gtttcaaaaa aacggcagaa gatatgaaaa aacctgcaga tgaattagga
    27781 agtattgcta aagatacttc agaagatatc ataattgatg taaaagacgc tgctggtaaa
    27841 gcaggagata ctgtcaatga aggaatgtca gaagcaaaac cagttgcaga tgactcaaaa
    27901 gaaatgatga aaatggcaaa agaagatgcg aaagaagcta aaaaagaaat aaaagataca
    27961 atgacagatt catctacaaa tcactaaaat cacaaattta tagaata
//
LOCUS       DAMEET010000031        27948 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.031, whole genome shotgun sequence.
ACCESSION   DAMEET010000031 DAMEET010000000
VERSION     DAMEET010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 27948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.031"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(414..1358)
                     /locus_tag="PB780_001198"
     CDS             complement(414..1358)
                     /locus_tag="PB780_001198"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDE0733263.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1654..2598)
                     /locus_tag="PB780_001199"
     CDS             complement(1654..2598)
                     /locus_tag="PB780_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDE0733264.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2819..4033
                     /locus_tag="PB780_001200"
     CDS             2819..4033
                     /locus_tag="PB780_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDE0733265.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASIYGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4055..6070
                     /locus_tag="PB780_001201"
     CDS             4055..6070
                     /locus_tag="PB780_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase"
                     /protein_id="HDE0733266.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEETFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6153..7526)
                     /locus_tag="PB780_001202"
     CDS             complement(6153..7526)
                     /locus_tag="PB780_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDE0733267.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7552..7836)
                     /locus_tag="PB780_001203"
     CDS             complement(7552..7836)
                     /locus_tag="PB780_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDE0733268.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7838..9853)
                     /locus_tag="PB780_001204"
     CDS             complement(7838..9853)
                     /locus_tag="PB780_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDE0733269.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10032..10337)
                     /locus_tag="PB780_001205"
     CDS             complement(10032..10337)
                     /locus_tag="PB780_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HDE0733270.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10426..11457)
                     /locus_tag="PB780_001206"
     CDS             complement(10426..11457)
                     /locus_tag="PB780_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDE0733271.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11664..12539
                     /locus_tag="PB780_001207"
     CDS             11664..12539
                     /locus_tag="PB780_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDE0733272.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12691..13533)
                     /locus_tag="PB780_001208"
     CDS             complement(12691..13533)
                     /locus_tag="PB780_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDE0733273.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13625..15502)
                     /locus_tag="PB780_001209"
     CDS             complement(13625..15502)
                     /locus_tag="PB780_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDE0733274.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     RPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEATIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIDNQTNTPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDHETSGTYAYVLYDG
                     DKEIDPTNYSVTEQTNGFTVSVDSNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15499..16920)
                     /locus_tag="PB780_001210"
     CDS             complement(15499..16920)
                     /locus_tag="PB780_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDE0733275.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQAATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQTEIAKNGA
                     SSGTFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKAPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGISTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16923..20312)
                     /locus_tag="PB780_001211"
     CDS             complement(16923..20312)
                     /locus_tag="PB780_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDE0733276.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDCVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNSWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKNLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20569..21960
                     /locus_tag="PB780_001212"
     CDS             20569..21960
                     /locus_tag="PB780_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDE0733277.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22033..22680)
                     /locus_tag="PB780_001213"
     CDS             complement(22033..22680)
                     /locus_tag="PB780_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDE0733278.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22688..23374)
                     /locus_tag="PB780_001214"
     CDS             complement(22688..23374)
                     /locus_tag="PB780_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDE0733279.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23380..24453)
                     /locus_tag="PB780_001215"
     CDS             complement(23380..24453)
                     /locus_tag="PB780_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDE0733280.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24675..25349
                     /locus_tag="PB780_001216"
     CDS             24675..25349
                     /locus_tag="PB780_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDE0733281.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQPPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25354..26694
                     /locus_tag="PB780_001217"
     CDS             25354..26694
                     /locus_tag="PB780_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDE0733282.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMATIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26843..27829)
                     /locus_tag="PB780_001218"
     CDS             complement(26843..27829)
                     /locus_tag="PB780_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HDE0733283.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDENLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8150 a         5279 c         5063 g         9456 t
ORIGIN      
        1 aacctctctc gaacggtttt ttatcaaaaa tcgtcatttt tccatcaaaa tctgcttatg
       61 caaaattata aaaaaatttt gcagtttttc ttactttttt tgtcgtttaa ttgaatgata
      121 aaaaccagtt tctttattca gttaatcaaa tatgaacgaa ttagatatga atcattgtat
      181 ttgccgttgc ggaaggattt tttaattaac cacagataaa cattgataac gattcgctta
      241 ttgtaatcaa cagattaaat ttcatatgtt catatgtacg gatacatttt cggaccgatt
      301 aattttaacc attctgttta tttgcttacc tattagatat caataaaaaa ctgcttaaca
      361 gctatttcag ctgttaagca gttttaggat ttttagttca tattcattgt ttcttataat
      421 acagtgttca atacttctct taaagcactt gttgatttgc tgaacattgc tttttcttct
      481 tcgttgattg ataattcaac aacttcacga acaccatttt cgtcaacgat tgatggcaca
      541 cctgtaaaga tatctttttc gtcgtattca ccagttaaat aagcagatac aggcaataca
      601 gcttgttcgt tattcaagat agcttttacg atacgtgcag tactcatacc aataccatag
      661 taagttgctt ttttacgatc aatgatctca taagcagcat tacggacttt atcagcaatc
      721 acatctaatt catcttgggc aatacgatgg tctttttcta caatttcaaa cactggttta
      781 ccaccgacag ttgtatgtga ccatgcagca acttctgaat caccatgttc acctaagata
      841 tagccgtgga cgctgcgtgg gtcaactttt aatttcaatg cgatttcttt acggaaacga
      901 gttgtatcca atgttgttcc agtaccgata acacgtgatg ttggcagacc agattcgttc
      961 caagcgatat atgtcaaaat atctactggg tttgaagcaa caacgataat accgtcgaat
     1021 cctgatccca tgatttcttt tacgatttgg cgcatgattg acgcatttgt tttaactaaa
     1081 tctaaacgag attgaccagg tttttggttg ataccagctg tgataacaac gatatcagca
     1141 tctttacatt cttcatagcc acctgaccat acagccacat tttcatcgcc ccaagccata
     1201 ccatctaaaa ggtccaatgc ttccccttct gctttttctt gattcacatc gatcaatact
     1261 aattcattag agattccttg gttgatcatt gcataagcga tacttgttcc aacaaaacct
     1321 gtaccgacga ttactacttt acgacttgtt tttttcatga ataaacagct ccttttcata
     1381 actatttgtt actttcatga aaagtatagt cttgtttatt cattttatca acatgttaat
     1441 tataatagtt gttattataa tttttgatgg aaacgaattc ctgaatacgc ataacagata
     1501 aatgacaaat gtttacacct atatcccctg ataatacaac caaaagttta gaactgaaaa
     1561 taaatccaaa aaaattcgct gcaacgatat aataagacag caatcgcacc taaaacaatt
     1621 ttccttcata tgctttagtg aaagattttc ttcttaatgt ttatttctcc attcattggg
     1681 agtacatgtt gaatacttct tgaataattt gaataaagaa gtcgtttgtt tatatccgat
     1741 acgattagcg atttcttcca acgaaaaact cgtttcttga atcagttcca acgcaaagtc
     1801 gaattttttc tttagaacca gttcttgaaa ggaagcatca aaatacattt tcaatttatt
     1861 actaagatag accggattgt agccaaaatg agctgctgtt tcttttaacg tacaagaagt
     1921 aaaatgctta tcgatataat ctaatactct aagcatctca ttttctgcgc atccatctgc
     1981 tgatgaaaag ctttgttgtt ctaataaatg agtcaattcg atcagtaata gtgatgtata
     2041 gagatttaac gaacggatct tatgactgta tgtatcaaaa taaatcaaca gcatattttc
     2101 aatcaaatgc gtcaaacgac ttgtttttcc gccgcgaaaa tgaatgaatt gtgtatgttc
     2161 ccctttttct tttgatgcat ttatcaaaaa agaagaaagt agattatttg tgcttgccaa
     2221 atgattcaat atctctgtag ataaggtttc ttcttttaat aagatgttga ttaaaatatc
     2281 ctcttgccct gtgtattcga ttctttgagt agcttcttta tcaatgatca ccacgtcact
     2341 ttcttccatc tctatccttt ctccattaat aatttgtaaa catgtaccgc tatacatata
     2401 attgatttca acaaaatcat gtgtatgctc gggaacttcc gcatatcgac ggtgtttact
     2461 gatatgaata tttccctgtt gaaatagatc ccctcggtgg atttttggcg gtgtctgaag
     2521 atggtaagct aagtttaaat cattgatatt cattccttgt tcacgctgca gagtttcgat
     2581 tgattcttcg ttacgcaaaa aaaatgtcta attcagctag ttccaattga ctccctcctt
     2641 tttgtaagcg gtttctttcg cttattagtt cacgctgtac tgctattata tgcaaagcta
     2701 tttttggttt cataaagact ttctatggtt gggacatgaa cttcctgtga ttccgctatt
     2761 gagagaataa ccacttcata ctatattttt gataagcgct tacaaaaatg aaaggaacat
     2821 gcttatgaat aaaaagatga tgaaattttc tctgctgtca gtctctctta ttttgacttc
     2881 tgctggtgca gtcacgtctg caattcccag tatgtcagct acttatgcca cgcgttcttt
     2941 atctgctgtt gaattattaa cgactgctcc ctcttttatg gtcactattt ttgttttgat
     3001 tagtagtttt attgctagga aaatcggaca aaaacaaact gtgatcatcg gattagtgat
     3061 tgcatcgatt tatggtatcc tccccctctt ctcacaaagt tacagtattg tcttagcttc
     3121 aagagctgga ctaggtattg gctttggatt gatcaattct ttggcagtca gcatgattgc
     3181 tgccttttat cacggagatg aacgagcctc tatgattggt tttcaaagtg catttcaagg
     3241 atttggtgca gctttaatga catttgttgc aggacagcta gtacagttcg gttggcaata
     3301 taccttttta gtctacgcca tcagcttacc tattcttgtc ttgtttgttt tgtttgttcc
     3361 tacacctgaa acaactgaac ttcaatcaca tggcacagcc ccaacaagac aagtaatcaa
     3421 aacgaatcct aaaatgatta gttatggtat ttttctcttg ctggtcatcg tggtatataa
     3481 tgctgtatcg attaaattag ccacagtcct gacgacttat tctctaggaa acgaaactaa
     3541 tgcagcgaat atcttaacaa tcatgcaatt ggcaagtatg gctgccggag tttgttttgg
     3601 ctggcttcag acaaaagcac atcgctatat gcttacactt tctttactac tgatgggaat
     3661 cggctttgta ttgattgcca ctgtgataaa cctgtatgtg attgggtttg gtgcgatcct
     3721 aacaggaatt tctttttcgt tattcattcc ttacttattc aaccaagtaa gtatccaagc
     3781 gcccaagtac gcggagagtt tcaatacttc aatcttattg gttggcgcta atttaggtac
     3841 tttcattgca ccatatggtt tgctctttct ctcttttgct tctcttggga gtaaaacact
     3901 tgctgttttc gtcaatggca gtatcttgct gttgattagc gcgcttatta gccttctagt
     3961 agtacacagg acgaatagaa agacagtgat aagagtagat gaagaaataa aagaacaatt
     4021 gacaacaaaa taaatttgat ggaggtcgtg taaaatgaca aatgaaaaac ataaagatag
     4081 atggctttgg tatcctggag actttgagat ccgccatgga ctgctacaaa atttccaacg
     4141 ggaagaacga ggttttgact ggccagcgta ctggtacatg gatgattgcc atcggaacgt
     4201 caagtttaag agatattact ttcttgatca accttccatg tttaaagtga cgatccaagg
     4261 agttgggtat gtagaaatta atggacaaaa acatccatgt ggaaaatggc tgacttgtcc
     4321 agctggtaaa gcaaaaattc gtatctttgt aggacataca tcagggctac cagcgatgtt
     4381 tatcgaggga gacgaagtaa aatcagatat tggctggaca gcaagtaatt ttattgaaga
     4441 atatccagct ggctggagtc cgctttatgt cgatatcgca aaagatccaa atcaaatcta
     4501 ttatcaaaaa gaatgcgtcc atccagtcac tgaaacggag attggcggag gaatcttatt
     4561 cgattttggg cgagcagtga atggctgtgt agaactaata tctctggatg accatatcga
     4621 atctatcact ctatgttatg gagagtccga tgtagaagct atggatatcg attattgcta
     4681 ctataagcaa ataggaatga caagaaaaat gaaagctcgt aaacgcgctt ttcgctattt
     4741 gtttatacca gaggtttcta ctggaatggt tcaacttcaa gtttttcatg aatttttatc
     4801 tctttcgaaa caagctgctt tttcaagcga ttcttcttta ctgaatcaga tctggtcagt
     4861 atcagaagaa accttcaagt tatgtagtga tttgtttttt atcgacggta tcaaacgaga
     4921 tcgctggatt tggtctggag atgcgtatca atcattcatg atcaatcaat atcttttttt
     4981 tgacgaggaa atcaataaac gaacgatatt agcattaaga ggacaaaatg aaatcaagca
     5041 gcatatgaat actatcgttg actattcgat cttgtggttg attggtatcg aaaaccacta
     5101 tatgatgacg aaagactatg aatttttacg catcgtctac cctaagatgc aatcattaat
     5161 gagatacttg atggagcaaa cgaatgaatt agggtttatt tatgggagag caaaagattg
     5221 gatatttatt gattggtctg aaatggataa agaaggaacg cttgctgcag aacaagtctt
     5281 attactgaaa gcctatcatt ccatgatcac ttgcggagat atcttagctg aaacatatgg
     5341 agagaacgaa aattatcata ctcatctcta tcaagaaaaa tatcaactat tgaaacaaaa
     5401 cctatttgac tacttttggg atgaagaact tggcgctttt atcgattgct atgaatctgg
     5461 gagaagaaat gtgacgcgtc atgcgaatat tttcgctgtt ttattcgatt ttgctaacaa
     5521 agaccaacag caatcgatct taaagaatgt cttattgaat gacactatca cacagattac
     5581 tactccatat ttcaaattct atgaacaaga tgctttatgc aaactcggac agacgaatat
     5641 cgtctaccaa acgattcttg attattgggg agggatgtta tctcatgatg cggtaacttt
     5701 ctgggaagaa tacgacccta ctcaaacagg aaatgaaaag tacagtatgt atggagatcc
     5761 ttatggaaaa agcttatgcc atgcatgggg agcaagccca atctatcttt taggacgcta
     5821 ttttatcggt ttgtatccaa caaagccagg ttatgaagaa tttatgatac aacctaatct
     5881 agattcattt gaaacactga attgtacgct acctatcaaa gatggaaaga tttcgcttga
     5941 aaaaacaaaa gataagatcg tgattacttc tgatagaaca ggcggaacag tgaaaatgaa
     6001 tggtaaaagt tatcctcttc tagcaaaaca gccacttgtc ttaacaagct attcaaaaat
     6061 aaatgcataa aacaaaaaac tgcttgcgac atatcagaag actttaaatc ttcttctgat
     6121 attgtccaag caatttttat tttgaagttt gcttaattct gttctttaga aacaatgttt
     6181 gttctttttc cggcaaaaag aaagctagat aatacgacaa gtaaaaagaa agcgatgatt
     6241 ccaactgtca aggctatttg accgtattct cctagtttcc caagtacaag tcctggtact
     6301 agataatcag tatcggcaaa tgtagaagaa gcgattttaa gatctcctaa tactggaagg
     6361 ataaataatg gcagccaact aatcaataag ctattgaaag cagaacctaa taccgcccct
     6421 cttctaccgc cagtcgagtt gccgaagaca ccagaagcag ctccgcagaa gaaatgtcct
     6481 actaccccag gaatgataac tgtagtttgc aagcctatca tagcaaacat ggatagtgtt
     6541 cctacaaaga agctaacaaa aaagccaatt aatacagcat taggtgcata tggaaaaacg
     6601 ataggaacat ctaatgctgg tttagaatcg ggtacgagtt ttttagcaat cccttggaat
     6661 gctggaacga tttctgccaa caccattcga accccttgaa ggacgataac aaacccagct
     6721 gtgaatgttc cagcttggat cagtgcaaag attacagcgt tgtctccatt gcttagttct
     6781 ctttcaacaa atccaggacc agctaacaaa gcaagtgcga catatacgat cgacatcaag
     6841 agcatgatcg atacagtaga atctcgcaaa aagctgaaac ttttaggaat ctttatgtcc
     6901 tctgtagaac ggtctttgtt tccaaaaaac tttccgatcc atccactaga agcataaccg
     6961 atatttccgg tatgccctaa cgcaacttga ttgtttccag tgatgttctt cataaacggt
     7021 tgacaaagag caggcgtaat agttaatagt gttccttcga agaccccgcc taataagatc
     7081 atccacaaat ttgcagtaaa ccccgcgctt actaaaatga ctgctgttag acaagaaaca
     7141 taaagcattg cttgacctgt aagaaaaatg aatttatatc ttgtgaatct agcaagtaaa
     7201 atattcacga tcatccctac tagcatgatc aaagcagtag gtgtaccgta ttgttgcaaa
     7261 gcaatcgcca ctactgcttc attgctagga actactcctt gagtatgcag agctgtttgg
     7321 aacatcgaag caaaaggggt tagtgaatta ctgattaaac cagcaccgcc gatcagtact
     7381 aggaatccga caaatgtttt gatagttcct tggatcactt ctgaaaattt cttcttctga
     7441 agaacgagac caagcagtgc aatcaaagaa acgaggatgg agggctgact gacgattgac
     7501 acaaacaaat tcaacaattt atccattgat agttcctccc ttgaatcatt tttattcttt
     7561 tgacaagatc ttcgttactt tttcttttac ttcttcgctg tcaatgatcg attgcaaagg
     7621 tacgagcttt tcatcaggta tagaagttag cgtatctttc aaagaatgct caatgaagaa
     7681 ataatcagcc atatcgctag atacacttcc aacatccgta tgacttactt caaactcgtc
     7741 cacatctgct ccaagatctg caagtgcttg tttgatgttc atctctacca taaaacttgt
     7801 tcctaatcca ctttgacata ctgcaaaaat tttcatatca ttcgtctcct tttcttatga
     7861 tcgctaatat ttcttctttt gttgatgcgt gtaataaatc ttgaagattt tgtttattag
     7921 aaagtagctt tgttaaattt gccaaagccc gaagatgtgc ctcattatcg atagcagcta
     7981 aacagatgaa caaactaatg ggatgggctg gattatcaag cagatccaca ggttgttgaa
     8041 gtttcagcaa agacatccct acttcgttta cgccatcttc tggacgagca tgaggtaatg
     8101 cgatgtgatc gccaatatgg ataaacgcac catactgctc cacgcgctcg atcatagctt
     8161 tgatataact ttcttctatt ttattttctg ataatagcgg ctgtgcggca aatgtgattg
     8221 cttcttgcca gttttttggt tgatccgata attggatcat ttcttctgtt aataattcag
     8281 atagcatagg acgttgatcc tccttctttt ctaaaagttt gttcatttta cgatttaata
     8341 cttgatagag cttttcttcg tctacgcctt cttttaattc gatataaggc agtagcgccg
     8401 acaacaattc ttgggcagaa ggcattgaaa atcctggtat cagccaatct ctttgtactt
     8461 gattgatcag ctgatttttt tccaagtcag tcatgattgg atgcagaaca tagagagact
     8521 ttttcttatc agtaagtgtg agaggtacag tagaaaaaat aatatcgtaa gaagtttcgt
     8581 caacctcgtg taagcggtca gctgaattag tctctgtaaa aaagatcatc gggaataatt
     8641 gctgaagttc actttttaag atcaatgaag agctaatgcc acttggacag acgatcacgg
     8701 ctcttatttt tctatttctt tcttctgcgt ccgcttttct gatctctccg ccaaatagta
     8761 tagtgaaaaa accaatctct tcttcaggga tgctcttccc tattcgtctt tctaacggaa
     8821 gtaatgcttt tcttgtcatt tcgtagatgg aagcatattg atgcttaatt tgatcgatca
     8881 tcacattagg taaataaaaa gaataggaaa tacgaaaata ggcagggacc aagtgataaa
     8941 agacattcat caataattca cgaaacgaag caaattctac ggccatcagg aattccatgc
     9001 gatggatgac ttctgaagaa cattccagca aaaaatcaag tgcaggctcc tggacatctc
     9061 cttcaacgat cgatgctaag caaagagaga aaaatataat ctccgaattt tgatgttcgg
     9121 aatagccaag gccagtcaat acttgtcgac taatgtcagt gactgcaaga gaagccaaca
     9181 gttcagcatc ttttaccagt tccatggatg tttttccaat tcgaatggtt gtcaaactca
     9241 aataataagc taattcatcc attcgatttg gtgcgaatcg aagatctgat agtgaaatcg
     9301 ttttatctag caaatctcta actttggcgt aataatcaat ggaaagggtt gcggttaaat
     9361 acgtgagccc aaatcttcca gcaagtgact gcatcaaacg agcagtgcga ttttttgctt
     9421 ctcttcttaa tattgcttct gaccctagca gcctaaaacc agttttccta gtatacgaca
     9481 gctgacaatc ttccacttct aattcatttc ttatcttttt gatatctgac aggactgtcc
     9541 ctttagaaac ctgaagaaaa tcttgaaaat gaaaaacaga aacagacgtt gcagtcgaat
     9601 aaaccatcaa ataaagaaga tctcttcgct cttgttcggt atacacagct tcaaatcgag
     9661 taatatccaa aaatttttct gcccagtcga ttgctcggaa tttctttata cggatacggg
     9721 tatcatccac atattcggca aaaggagtta attgtttgat cgcctcgctt gtatttactt
     9781 cgatgatctc tgctgctttt tctagactga tttctcttgg tgttttcagg agataactaa
     9841 ttttgtagtt cacagcgctg ttcacctcct acttatattc tagtatggta gcgcttttaa
     9901 atcgaggggt gtttggtaca acttacagtt ggattttttt tacgactgta ccgaacgatt
     9961 tctatcgaat ggatacagaa aaaaagcagg tgaccaaatg tctcctgctt ttatgaattg
    10021 atttcgtccg atcaatgatg atcatgttta ttttttgagg tcagttcttc aaatttttct
    10081 ttgacatctt caaccgcttt ttcgatatct ttttcaaagt gattgggctc attaggcttt
    10141 gtttcctctt ctacagaatt tgaaccagat aaagctgttt ctttattttc tgtttcacca
    10201 gagtttgtgc cagctaatga ttcttctttg tcttctgttt caccagaatt agttcctgct
    10261 aaagccactt cttcttctgt tttaggggcc attgaacctc ttaatcgttt gtgtttttca
    10321 ccgtcataat ttgtcatgtt ggttcctcct agtagttcta tactttttca tcgtacaata
    10381 agacagttta tgcgtcaaaa aacaaacccg attcttacaa atatgtcata tttttccttc
    10441 tgattcttga atgcttttga ttccttcttc caaaattttc atttgtaaga ttgtttcttc
    10501 atctttgatg actttttctg cgccattaac taatgtctcg taaatccctt cgtagacacg
    10561 gctgtaatca ccaacctcgg aaattacttt ttcttcatga taactccctt gttcatctat
    10621 ataggtcaat acaccataat gttcgggcaa atcgatacca aagtcttcat gatcaggcat
    10681 gtaaaacatt ttcagatgtt cttcttgtcg atcttttgtt tgtttcacga acatcccttt
    10741 ttttccgtaa acgacgaaac ttggacgttc tttgatgcgg aaataactgg attttatcga
    10801 aattttcata tttccataga aaaaatccag atcgaagtaa tcattcattc gtcctttccc
    10861 taaaagttga cgtacatcat agctgatatc atctggttcg ccgaaataag aaagaacttg
    10921 atctactgta tgacaagcat gtccatataa atagctgttc gcagtagaaa attcatgcgt
    10981 attttctggt acttccggtc tgaaataatc ataatgcatt tctacttcta aaatatcccc
    11041 taattttccg ctttcgatta ctttttggac tgtcaagaag tcagaatcga accgtctgtt
    11101 ttggtaacat tggataaata gacctttttc ttttgctaat gcaaataact cttctgcttc
    11161 ctttgacgtt tctgcaaaag gtttctctac tagtacattc ttaccatgtt cgagtgccat
    11221 ttttccatat tcataatgaa attgactagg tgtagtgatg acaactaatt ggatctcagg
    11281 atcatcccaa agatcagaca gctgatcagt atattcgatg tcttcgaatg ttttccacac
    11341 tggttcagat ggggaaaaaa tttttttgat attgattttg ttcgtaagtt ttcttgaaaa
    11401 aggcaaatga tagcgatttg tactctttcc attgccaaga taagcgattg taagcatgag
    11461 tggtgcctcc ttttattgat gttttcagta tatcctagaa aagcgcagaa agtatttata
    11521 atagctattc tggtaaaaaa gttcagaaaa acacttgtta tcattcggtt tggtgaaatt
    11581 aatgttcaaa gtaaggataa aaatcaaaaa gatagtagcg gaaccaagtg tttatcagta
    11641 agatagaatt tggaggtcag atcatgctta ttgaagaaaa actaacaaaa caagagttgt
    11701 ttaccacgac agagaaacgg attgtcgatt acatccgtag gaatatcgaa gctgcagtct
    11761 acatgacgat tgaagaatta gcaaaagcta cgtatacttc ccattcagct atcattcgtt
    11821 tatgcaaaaa aatgggattc agcggattta aagaattccg tttcgagctg gggcgggaag
    11881 ttcatcaatt gattgctcag ttcaatcaaa cgattgatcc taattttcct ttttcaagtg
    11941 aagatagcac tcagcaaatt gcaaagaaaa tggcggattt atcgatccag tcaataaaaa
    12001 aagcacaatt gcagattgaa catgaagatt tagatcaaat cggtaaagta ttgaacaagg
    12061 cacagcgaat ctttcttttt gcaaaaggtg attctcaaat cacggcaagg aaattccaga
    12121 ataaaatggt caagttgaat aagtttttga tcatggctga agaatacagt gattcttctt
    12181 ggaatgcagc caatttgact agcgaggatt gcgccatttt tattagctac agcggtcgca
    12241 tccatcatta tgaacgaata atgacctatt tgaagcatgt agaggcccct accttgctga
    12301 ttaccggaaa tcagcattca gaaatggcca aacaggctga tatgtgtctt gttatatcgc
    12361 aggaggaata tgattttgct aaagtagcga ctttctcctc tcaaattgcc ttcgattacg
    12421 tattaaatac gttattctca gtgatatatt ctcaaaattt tgaagagaat atccgcaact
    12481 tgaaaaacaa acaagaattg atgcaaaaag gccttcttaa agaatatcgg aaaacttaag
    12541 aaaagatcct catttagtaa taagaaagat agtgtttgat aggctctctt tgatatcatc
    12601 ataaaaacag gctgaagttt tctttttcaa acctcagcct gtttgtttac ggaacgattc
    12661 gaatagatat attatagaaa tgaggaatag ctaacttctg tttcttattt tgaacgattg
    12721 acgaaaaaga tatataagaa ggaggaaaat cagtatacat gcagctaaca atccatacag
    12781 gaaaaatcgt ttgttctcgg atactttctg tacttctttt tgaacttctt ttgcatgata
    12841 aggaacccgg tgccctctga caagtaagcg atgactgttg accatataag gtgtacaagt
    12901 taggagtgtc acgaaatcct gccctttttc gattctcaga tcttttgtat cagtcggttc
    12961 taccgtcttg atctgatcga cttgataagc gagtgtccgt ccattgattt cgatatagaa
    13021 agaatcacct aaagtcagtt tcggtaagtc tgtgaacaac tttgcttgag gcagtcctcg
    13081 atggctggaa ataacggcat gtgtattttc tccccccgta ggataagacg ttccttccaa
    13141 taatgaggca cctttttcta gcagcacagt attcgtttca tcaaaaattg gcaaatggac
    13201 attgatctca gggatcgtca aaataccgat cgtatgtttc tcaaaatagg ttctatcctt
    13261 tttcgcaggt tcttttttct tagtaaaggg atctgcacct ggatttccac cttcttttgc
    13321 taattgctgg tttttctttg tcatgttttc ttggatttta gccatgactt cttcattttc
    13381 tttgaccgct tgttgctgat aatgagagat gatctgctga tccagataat tattcagcgc
    13441 gtcgctaaca aatggataag ctagtatccc tattcctgag agaagcaaga aaagcatcag
    13501 cagatctaga agtttccttt tctttggttt cattcgtgtt ctccttgtga tgactgtttg
    13561 ataataaaag aaaaaggagc atttgcgtaa tccgctgctc ctttttccaa ttcttgcaaa
    13621 aagactaaat ctgactgtgc ttgcgtctag caaagtacag tccagcaatc agtagaagga
    13681 ctactcctgc accgatatac acatagattc ccttaccgcc tgttgaagga agtgtacctt
    13741 tgtgtttatt tggtattttt tcaggagaaa tcaactgtcc tgctgttaca taagattgtt
    13801 catcgatagt aaatgctaca cggtttgtta atggaacata tttttctggc gctttcgttt
    13861 cttccagata gtacgtgcca tattttagac ctgtcacatc gattaaaccg ttacttgtgg
    13921 ttgtaaaaac cgttgctgat tctttcgccg atacccagct gacggctttt gtggatgggt
    13981 cgatcgataa atatttcgct gtgtcactat cttgatcacg aacgacgaat tctgctccag
    14041 caagtgtttg atctgatgta acgtcaccat ctactttaac aaatcgtttg cccccagtaa
    14101 cgacatcgac tgacggtggt gtttgatcat ttgtatgccc gttatcgaca tttgcttggt
    14161 tagaaaatcc tttggttgga tctgcttttt cgttcaaatg catatagtaa acaaatttca
    14221 gcgtaccacc aggagtgagt gaaggaatat aattcgagtc aactgaaacc gtgaatccgt
    14281 ttgtttgttc agtgacagaa taatttgttg gatcgatttc tttatcccca tcatacaaga
    14341 catacgcata cgttcctgaa gtttcatgat caaatgtcaa agcggcgtca tgagtatcaa
    14401 tgagtttgaa tgtcgtgtac ttgttttgtg ttccttcttt atcagcgata cctaaaggaa
    14461 tattgacaga gatctcatat tggatttttt ctccgattgc gacatctttt ccattcaatt
    14521 gaggtgttgt tttatcaact ttggacgtat catttttgat tgtcttttct acaggagttt
    14581 gactatttgc caagatctca aacggcgtgt ttgtttgatt atcaatcatt tccgcattat
    14641 caggagcttt tacttcttct aaattatatt ttccaacctc taaatgattg acgatcaact
    14701 gacctttttc aatcgttgcc tcggcaaagt cattgttgcc gatgtcataa gaatgaccag
    14761 taatgaaatg tttggctttg gtttgatccg ttgtccaagt gtacaatcca tctgtgacac
    14821 tttggatgta ttttttgacg cttggtcttc cttcttcttt agaaataata aattctgctc
    14881 catttagtgc ttcgttttcg gcagtaccga tttttgtaac tttcaacgtt ccatcattac
    14941 cgactgtatt tttagggtag agatggatag tatctagttc ttctgtcccg tatttataag
    15001 agccatctgc ttgtttgatc atctcatata cagggaaagc taaaaccatg tttgcggcag
    15061 ctgacactcc gtcttttggt tcttctttga tcgtgtagac tgcatctttc ccattttgtt
    15121 ttttaggtaa agataaagtg acatttccat cagcatctgt cgttcctgaa gcaactggtg
    15181 taccaggagt caaagactgg actgcttgtt ttgctgcatc cacggacgct cctttatctc
    15241 gttgcgcata aaattcttga gtgacgttat aaactgaaaa tgaaatatcg gctaatcctt
    15301 ggtattgatc gaattcgctc atttctttcc cgctgttttg gattaaagga tcgggtaaat
    15361 cagtcatttt ctttttatga aggatgactt gagctgctgt ctcctctgca aacacttttt
    15421 ttgcgccgaa gctgtttgtg agtaatggta aaataaggaa aaggactcct aacataacgt
    15481 ttattttttt atgatttttc attttttcac tcctgttctt tttttgataa acaaccagat
    15541 tgcaaagctt gcgaatacca taccaacaac gcctaaaagt gtatttttcg tttcgtttgt
    15601 ttttggaaga aatggacggc tattttcttt tttattgact actttcaata tactgtccgc
    15661 ctctttatcc tcagaaagaa aagttgaaat ggtaaacgga atttctgttt cacttaaaat
    15721 ataaccagaa ggtgcttcga tctcttttaa tcgataactt cctgttttca acccggaaat
    15781 tgaaaaagcg ccatttttat cggaaatcag gtgaagcgca tctgattcgt tctttgtcca
    15841 cttgtatccg ttggctgttt gctggaggta atttcctgct ttgtttttaa taaggaatac
    15901 tgcgccagct aatcgtttat ctggtttatt tgtctcgatt ttgacaaatt gcttactccc
    15961 tgtatataca gagactttct ttgtactgat accaaaacca aaatccagtg ttccgttgtt
    16021 gttgatttcc ttgttcgcct gtgctgtact gcttagctgc atctgataag aaatggtcag
    16081 tttttttcca gcatattttt ccaacgaagg gatcgaaaat aaaacatcaa atccgtgagg
    16141 ttttttgtgt atgacaaagc cttctgtcag cttttctcca ttcgatgaaa tcgttaaact
    16201 attaggtaaa aacgttaatg cttcatccgc actatctgac aattcgaact tttgatagtc
    16261 aaggatattt acaggaattg tcgtatgcaa ggaataagtg attttttcac caatcgtaaa
    16321 atcatttcta ggctcttcta ataccttctc aaaaggtgga tcagggtagt cgttttcttc
    16381 atttttagga tacaaatgga tagttgaaag tttttgattg ttttgtccat gtacaggaag
    16441 aacaactacc atattctctg ccttttcttt gacgacttct ggtgctttgg attcaatgaa
    16501 aagatacgct ttatctcttt tttcttgatc tttagcggcc agagaaaaag aagcgatacc
    16561 atcttcgttg ttaagagttg tagttgttgc ttctgcggta aacgtaccgg aagacgcacc
    16621 gttttttgcg atctctgttt gtgcttcttc taccgttttg cccttttccc gtagatggta
    16681 aaaagaatct gtgacatcat aaacttggaa tgtgacacca tttaatcctc gatacgtttg
    16741 taataacgct ttttcttgtc cgtcatttgg atgatttttc ggcagttgcc cgtttgggaa
    16801 aagcaatttg tgtaaagtaa tctgaaccat ttctgtttct gtttctgttg ctgcctgagt
    16861 aaaagctggt ttaaatagta acaagaagag acacatacta agccaaccaa gttttttcat
    16921 atttagctgc cccctctttc ttttttcaaa aaaagatacc aaatagaaaa cgcacaacca
    16981 atcatcccta ctagatagat tcctaaacgg ccaattcctc ccgtttcagg taatggtact
    17041 tttgcctgat tcgtgatgtc taaagaaatc tggttgtttt tggctcctgg tgacagaaca
    17101 gattcatgat cttgtccatc cacttgaatt gacccatctt cgtgtataac tatagtaact
    17161 ggctctttta gaccttggta tccttctggt gtaaaagttt cggtcagcgt ataagttcca
    17221 ggttttaaat tttcgaatag aaaggtatct gtttcttgtc catcttttgg cgattctaag
    17281 tccatttctg gtccctgcag tctgaatttt gctcctttta atgtctgtcc gttgtcttct
    17341 tttttgtgta ccgttagatc aaacgctttt agtcgatttt gatgagtgag cgtccataca
    17401 ttatttgtca tctcggtatt ttttcccgtg taaatgattt ttccatattt atcgatttgg
    17461 aattcataat ttcccggcga agtatcatat ccggcaggac cagctgtttc aacgactcgg
    17521 tatttaccag gtgtgagtga atcaaaactg gcaagacctt tttcattcgt tgtttggctg
    17581 tcaattggct gataagtacc atttgcttct tttttctgta gggaaaatgc tgctccttta
    17641 agatttatct ctttgtccgt cccttgcatc gcatacttac gtactgctac aggaacttca
    17701 acaaaaggat tttcaatagt caactggatc gtatcatcgg aagtagtgac tgtttttcca
    17761 tcaatggtta ccgtaccatc agaagcgatc ttgatttccc aagtagtctt tttgcttaat
    17821 ccatgccctt ctggagcttt tgtttctgtc agcgtatagg tcatttcttt ttgcaatttg
    17881 acattttctg gaagagaata ggttccgtcg ccatgatctg ttagtaaagt atcaatagaa
    17941 tctcctgtta atttgaagac agcaccgatg agattctttt cacctgtaga ggaatttttc
    18001 gttattttca agtttaacgg tgtgaattgt ttagtatttt tccatgtcat tgcatctatt
    18061 tgttgagaat cgtatcctgg tacaggtaat tctttgattg tttggtaact gaatgcttga
    18121 ccttgattat tgtattgagg tagtgataat atctcttgat aactttctcc gctatttgca
    18181 gataatttgt caatgtctgc acgttcccac gaatttgtag tatctttagc tggtttaatg
    18241 attcgaatat atccgttttt ccaagctgca gcatttgtcg tatgttcccg ttgaatctca
    18301 aaagtaactt gatctggacg atcagctaga ttgttgtcaa attcttccca taactttttg
    18361 atgtgaagac tgactccggg agctttagca gatggtatac cgaattcagc taattcattg
    18421 gtatcaatgt ttggctggaa agttgtcctg ccgttcattt gataccagaa atttggatgg
    18481 aagtcctcat tttctgtttg gattctcact tggtaatgga tttggatttc ttggtctttt
    18541 cctaaataga tctgattgct cttgatggta tttccttcta tggaaataga tggagtgacc
    18601 gttgtaggac ttgtccccac actcttgact gaaagtgtac caggctgata aatgaatggt
    18661 tctgcgattg gatcatttat ttgtccattg cttacagttg ctgagatctg tacagctttt
    18721 ttggctaggt attcagagat atctgttgca tgatcagctg attcatagta aagatcgcct
    18781 ttttcatctg atgaaaccat ttgtcgcata cgagactcta cttctgcttt tgagagacca
    18841 gctgccggat cgctttgaag ttggatgcca agaccatgta tttcgattcc tcgttctttg
    18901 agtgccatcg cttctccgat ggttgcgata aacgtactgt cgattcttct ggatagattg
    18961 ttttggtcag gtgcatcata gattcttgag attagagaag tatttcccgg ccgatcttgc
    19021 gtattagaaa actgagttcc gtaataattg ctgcttgatt gtgcgtgtac ccgctgtact
    19081 ttataggaaa atgttggtac acaatccgtc agcaaaacga tcactttttt atgtccattt
    19141 ggaacggata gcatgcttcc tgcatctctt agtgcttttt gtgtaaaagt accaccattt
    19201 gtccgtgaag gtgtaatgga ttttacttga tttttcactg aatcaaatga acccatctgt
    19261 actgcaccgt tactgtagct atatccttcg cttgagtagc cgacatatcc catattgatt
    19321 ttgtctgtga taccgctatc tgctaaagta tcgacaaaac gatcgacacc aatctttact
    19381 tcaccgatcc gattattgtc gttcatactt cctgaccagt ctacgaccaa tacgagatca
    19441 agaggcgtga tatctttttg tacatttcca cgagcattca aataaacatc aaacaatccg
    19501 ggtgtacttg tttcctttgc atactttcgt atcgcatagt ctgcttgatt accggttcct
    19561 ccatattcga tatacgaatg ggtccgatca ttaggatctc cattccaaga atttatacca
    19621 tcccattgat ttgttccgtt tttattgcct tgatgatcaa gaacatttgt atttcctgta
    19681 ggttgccaac tggctttagg ataagtgccc tgatcatctg ttgtatattc tgtaggaatc
    19741 gtttgattag atggcacact cacagctggc aaggataatg taggcaatgt aaatgggctg
    19801 acagaatttt cgttttcttt tgatgattct tcttctattt cgtctggctt tggtactgac
    19861 acatctgtat ctttcttatt ttctgtctca tcaataatgt catattcttt attatcgacg
    19921 atcagcttgt ttgcctggct gtggtaaagt gctggatcga ttggaaaact cagatcgata
    19981 gtttgtttag tgtttgatga gatttccaac gtaattttcc cgctatttac ctgataagca
    20041 tagccatctc cttcttttct ttccacatca cttaagtaat tttcgactag accaatttct
    20101 ttttctatct tttcagcact ggtgttattc gcatttagct tgatgttcag tgcttttttt
    20161 ccagactgat ccgaaatgac ttcagctgaa attgtttcta aaactgttgg atcatctgta
    20221 gtgtctgcta ttgcagataa tggcacaaag tttgataaac tcaatattat cagcatgaaa
    20281 ataactttca gtttcttccc tgtggtcgtc atttttcccc tcctttttct ttttttctgt
    20341 tttgacgtga atagtgaaaa ttattaaaat aaacaggcgt tacataattt tatttatttt
    20401 aatatgataa ttattatacg aagggcgcta aagggaatca caccaatttt cataccaatt
    20461 ggtttcattt atgaagttga ttccttcgat taatgagcgt ttcattgaaa gaaaagggtt
    20521 gagggagaaa aacatatgat caggttgttg gaatgaaact atagaaagat ggaaaaaatc
    20581 attcgaattc tgtccatttc tgacaagtgg atgactaaaa aagaacttgc caaacgaatt
    20641 ggtagttcag aatctacatt tgtaaggtat atcgaagaga tcaaaatccg ctggggaacg
    20701 gatgtaacga ttcaatcttc acaaaaattg gggtatcgtc ttgaacattt gaatgtacca
    20761 gcttatttaa atgttctgat ggaaatgtcg caaagttcta caaacagtcg tttattaaat
    20821 gagattattg tcaatccggg taaaacgatt gagtattatt gtgaaaaaat ttccatcagc
    20881 cggtcttctt ttacacgtaa gttgaaacaa tgcaatcaaa ttttagaaag ctatttatta
    20941 aaagtgatcg ttgaccaagg ttttcagtta actagcctta aaagtgagct ccagttgcgt
    21001 atctttgtca cctttttctt tttaagttac tacggaagac gcacgttgcc atttccaatc
    21061 gaaaaaaaga agatcaaaga aattatggtc aagaataatt gtcagatgaa tttgtttcag
    21121 gaagaaagcc catatgagca atctttcttt attatgtatt ttgttgtgca tctcatgaga
    21181 gaatcccaag gaactccatt tgaatggaaa gctggagact cttatgtaca gttgataaat
    21241 atcgataaaa aagacttctt aatcttgaaa aatctcttag gaaaattgaa gtacagcagc
    21301 tatataaaag tagtgaacta ttttgtcaat acgatttttt cagaattttc ggagaaagaa
    21361 aaaaatgaaa tcagtgagca gatacgaaaa aatatgtcct actgttcgtt ttcatccgct
    21421 gattgcaaag aaaaaaacga agaatatgtt gttcaattat taacgcaaat ttattttttc
    21481 tcccgtattt tcccatacga tacgactcat ttgacgatcc gtgcaactta ctttgcacag
    21541 cagatgcaac accgccagat acgtctatat gctggatttc gtgcagaact tcaattttat
    21601 tccactctac tgaatcagaa tctgatttat tatttatcta gctatatctt ttggatgctt
    21661 atcggtaatc ctgaaattca ccattatact tctgataaaa aaatactgat tatcagcgac
    21721 ttaagtcttc gacatagtca atatattgaa gaatatatca gtgatattct tgctgtgcat
    21781 aaaattcact ctgagacaac tgctattact gaagatcagc tgtcgaaata caatcttctc
    21841 gagtatgatc tgatcgttac gaatcaaccc attttgaatg cccatgttcc tcatatattg
    21901 attgatgata gtgtgtcatt cgcaaatgaa gaagaactca tccgcttatt cgagttatga
    21961 ataatcaaaa aacagctatg tataaaccac cctgataaaa atttcaggac aaatacacat
    22021 agctgtttct ttttattgta ttccttcagc aatccaacga gatttatagc gaaagtcttt
    22081 taacaactct tcttttgtcc aattatttgc aatgagatcc gtcaaagtgt tcattacgag
    22141 ggtcatggaa aagtatagga aactaactgc ttcttccgac gattgaatgg agaaatgatt
    22201 gattcttaat aattccagcc atttttcgtt cattggagaa tcagccgata gttctctacg
    22261 tttggttgtc tgctgatcat ttcgctgggt taacaagtat aacctttgcc aatagatact
    22321 ttcgtgatct aagtcgcagc accactctaa atatttttcg atccccaaaa agaaatgttg
    22381 tttgttttcg ctaatgaatt ttaagatatc ttgatggact atgtgagaac tgcttttgat
    22441 cgtatacaaa taagcatctt ccaaatcttg aaaatattta taaaaagcac cgcgcgacat
    22501 ccccatcttg tcaacgattt ctgagacttt gacttggctg atgtgctgtg agcaaaatat
    22561 ctccagtaat aggtcatcga ttctctgttt ctttgcgttc gataaattga aaaaagtttc
    22621 ttttggcatc cacatctctc ctttatctct gattatacgt ggatagccct tcattgtcat
    22681 gaaggattca ttccttcttt ttctcttctt ctgtcaagta cccatcttcc attcgataaa
    22741 cgcgatcact ccattgaatc atacggacat catgagtaac cataatagtc gctttttgtt
    22801 tctcatgagc ttctttaact agtaatttga cgacttcata tgcatgatct gtatccaaac
    22861 tagctgttgg ttcgtctgct aagatcaagc ttggctcatt atataaagcg cgagcaattg
    22921 ccactctctg tctttctcca ccagaaaggt ctttaggata actattttta agtgactgga
    22981 tatctaaaga ttgcagcaag gaatcgattt ttgttgaatc ttcttttttt gaagtaactt
    23041 ggtcaactag ttcaaattgt tcctttaccg ttaaaaaagg aatcaaatta gagctttgga
    23101 gaataaatcc tatttcttta aagcgtaaat ctgctcgttt tttttctgga agctcagtta
    23161 gtgagtgacc gttgatcact atttctccgg aagtcggtgt ttgcaatcct cctgaaatgg
    23221 taaggaaagt acttttacct gaaccagaag gaccaatgat gctgacgaat tctccttcct
    23281 ttacataaaa atcaattgct ttcaaagctg agacctttgt atgaccagaa ccaaatgttt
    23341 tggtaacttt tttcatcgtt aatatttttt tcatttttat taacctccta ttgctgtaat
    23401 tgggtctact ttggttatcg tacgtatcga aaacaatccg cctagcattg cgacaatgat
    23461 caaaatccca ctatatagca accattgtga ccaaaaaaca gcaaaaggca tcgcgcttgg
    23521 taaaatcaga ctagtcaaat aagcaaacaa aagagcaagc agaacgccta ccaccccaac
    23581 aataaaggac tgtgctacta aagatttagc gatgaagaaa tttttgatcc cttgtgcttt
    23641 cattactcca aaaatagcag tcttctgaag tgtcatcacg tacatgaaaa taccgaccac
    23701 ggcagcaacc actaagaaca aaaagtatat catcgcattt aaggtcatat tttgggcaga
    23761 atagccagga atactttcaa tgaatgtccc aatagttaat aactgtagac ccttcgatgc
    23821 tttttctttc gaaattttag ctgaatcctt tattacgatc ccattgatcg gctgttcttc
    23881 ttcagaagca aacggctgtt gtccatactt caaagcagtc cacgtatcta gggacgcata
    23941 gatgacggga gaaactgtgt aataagtttc aggaaagata ccgacaatcg tcaaaggatc
    24001 gtcgtaactg ccaattttga tcgtatctcc aattttatag cctgaatcag ccagattttg
    24061 agagatgatg atttcattct ttgttttaaa ttcatgccct ttcgtgagtt ttggtaataa
    24121 aaatgcttct ttcgttgtgc cgaaaactgt aacattttct ttgttttttc ctttgattgc
    24181 tccgctgtac aatccgattg gtgctttctc tcctccttca atgtatttca gatctcctct
    24241 tgttaattgt gaagctgcaa atacttgatt tgcatcctcc gacaaaacaa tctcttgtgc
    24301 gtcccaatca tcgattgctt ttttaaactc ttctgaaagt ccattcgcta gtcctgacaa
    24361 catgaatacg acatacgcaa tcaagaacat aataccgacg atcaagccat atcgtaattt
    24421 agaatagcga atttctttga tagctagaaa catttgtttt tcctccctat gatagattga
    24481 tagtgacaaa atgtcacttg ttgaatagtg taactctaaa gtgacaattt gtcacttgtg
    24541 ttgttttttt cttatcaata aattgtctaa gacatctttc gataaaacct taacatgagt
    24601 cataaaaaat ttatttaagc cttatttatg ctttccatga taaactaaag tgaccaaatc
    24661 aatgaggtgg gaaaatgatt aaactattag tagtagaaga cgataaagta ttatccaata
    24721 atataaaaga aatactcgct gcaatcggcg agataacaca agtttatgat ggtagtgaag
    24781 gactgttcga gttggaaagt ggagtttatg atcttgcaat cttggatttg atgatgccgg
    24841 aaatgaatgg atatgaagtg ttagcaaaaa tacggaaaga aaaaatacag cctccagtat
    24901 tgattttgac tgcaaaagat agtttggaag ataagattga aggatttgaa cgaggtgcag
    24961 atgattatct aacaaaacct ttctatcgag aagaactgat catgcgcgtg aaagcattat
    25021 tgaaacgctc gctaggtctc tttgatgaaa atgttctgaa gtataaggat atcagttgtg
    25081 atttaaaaag caatgaagtc ttgtataaag aacaagtttt gcccatacaa ggaaaagagt
    25141 ttgaattgct cgtttatttt ttacaaaata agggaatcat tttgacaaaa gagcagatat
    25201 ttgaccggat ctggggattt gattctgata cgacgattac agttattgaa gtttatatga
    25261 gcaacttacg aaaacatctg cgaagtactg gattggataa agaaatcaaa accttacgta
    25321 atgtcggtta tatactgcaa agggtgtgat gcaatgaatg gagatattac acgaaagcaa
    25381 cgtagtcgct tttttgcgac aaatgtagca gcatttgctt ttatcttttt tcttttggga
    25441 ttcattactt tacagatatt aaaccaatct gcatacagag aaacagatct gtcattagag
    25501 aaaataaatt ttgattctcg aacagtacaa ttagagatac aaagatatca gcaaggcgat
    25561 ccctttttaa acccgactca aacggaacaa aacagaacaa acatggaaca gccattaatg
    25621 gaaccatcaa atgaccgttt taacactcaa gtgatcttgt ggtctaaaaa tggtgagata
    25681 ttgaataaag cagctattgg tgggcgtttc aaccaaatca aagaattaaa aatggatacg
    25741 aaaaatctga ataccatcca aaatattgaa ttaaatgaag acagtggctc atctgccctc
    25801 tctttccgtt cgattacgaa aaaagcggag acgaatcaag atggcgttgc ctacatccaa
    25861 attttagaaa atacaaatca aatcaagaat tcattagcga cttttcgcac gattatgatt
    25921 ttgtgtatga ttgttttttg gattctctct attggtatca gttattactt gtctagtcta
    25981 agcatgcgcc ctattattct ttcttggaga agacaaaaag aatttgttga gaatgcttct
    26041 catgaattgc gcacaccgtt aacgattatt caaaatagtt tggaacatct ttttaccaag
    26101 cctaatcata ctattattga agaatccgaa agcatcgctc aagcattgtc agagacgagg
    26161 cgcctcactg gattgacttc ggatttgctg acaatcgctc ggaatgattc tgatcagcag
    26221 ctgctttcaa agcaaatgat caatacacaa gaatatatcg aacaacttgt taaaccattt
    26281 caagagatgg ccaccataga tggaaaagag tttattctcc ataacgatgc aacattagaa
    26341 gtaccaattg atgaaaaaaa aatccatcaa gttttggtta ttttgctgga taatgcatta
    26401 aaatatacga atccagaaga taagatcatt ttggattcat ctgttaacca taaagaatgg
    26461 ctgattcacg taaagaatac tggcccttct atcagtgaag aagatcaagc aaggattttt
    26521 gatcgatttt atcgggaaga tcaatctcgg gcgaaagaga ctggcggata cgggcttggc
    26581 ttggcaattg caaaacaggt aatcgaacag catcatggac agatttctgt atccaattat
    26641 cttccacaag gagtcgattt taaagtccaa ttgccgttga agcctaaaaa ataatgctta
    26701 tccctaaaaa agggctgtga caaaaatctt gtcacagcac ttttttgttg cgaataaacg
    26761 gtgttccaga agtagcttct tcggaaataa gccgaaattt ctttaagctg accacttctg
    26821 tcacaacctc tttagcttac gcttacgatt ttttattgcg gtcttcaatg tgtacatcac
    26881 cgcttgtgtc aatatccaat tcttctcggc taacatcttc tgtcactttt tcaacatctt
    26941 catgttcttc tttatgaatc ccaacttctt cacgaataac tgtatgtttg cttacatcta
    27001 cttgctcttc tttgattggg atagtaatcg tgtcatcttc caagttttcg tctgttccct
    27061 gtgaagattg atctgttact ggtttgcgtt cgatgacgat ttcttcacgt ttgaccggta
    27121 cttctaccgt ttcagtatcg ttgaccacat gtttgtggat gtcgacttct ccagttgtca
    27181 catcatgtgt atttacatct aatttttctt cttttagttg aattgtatca tcatctagat
    27241 ttggtttgcg atcgatgtct tcagtcttgc ttgtacccat tgtatcagtc tcgccaggta
    27301 cgtccatcgg atcagttcct ggtgtcatat tcaatggatc cgttgtattt tgaaagcctg
    27361 tcgtgccacc tacgtccatt ggatctgttc cagtgccagt ttctaatcca gtccccattg
    27421 tgcttgctgg gctaacagta tcttgtgtat ccattgtatc ttctgttgtt tctggctgac
    27481 gatagccttt tactgtgatg acaaattttc catcagaaat atctcctcta tattgatata
    27541 aaggatcatt ttctggtgta gccgtctcag aatcataggt atcaaaagaa aaagtatctt
    27601 ttattttttc ccataaagaa cggtcttctt caccatccat ccgattttca tctgtttcaa
    27661 catctatacc agacaatccg tcaaatccta gtctagatgc tgcttcatca gtagtataaa
    27721 taacgatatc atctcgttca tacccttcgt tacgcaaccg ttcaattact gataatgctt
    27781 cttccctcgt actatagctt ccgatgaccg ttaaattttt ctcttccatg taaattcctc
    27841 ctagcaaata gatttatgat tggtatattg ttacttataa atttattcta taggagacga
    27901 ttgttttcta caaatattta gattggaatt tgattaaaac aatttaat
//
LOCUS       DAMEET010000032        27835 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.032, whole genome shotgun sequence.
ACCESSION   DAMEET010000032 DAMEET010000000
VERSION     DAMEET010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 27835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.032"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            331..2070
                     /gene="yfcC"
                     /locus_tag="PB780_001219"
     CDS             331..2070
                     /gene="yfcC"
                     /locus_tag="PB780_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDE0733284.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2227..3411
                     /gene="iadA"
                     /locus_tag="PB780_001220"
     CDS             2227..3411
                     /gene="iadA"
                     /locus_tag="PB780_001220"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDE0733285.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3522..3725
                     /locus_tag="PB780_001221"
     CDS             3522..3725
                     /locus_tag="PB780_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDE0733286.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3826..4488)
                     /locus_tag="PB780_001222"
     CDS             complement(3826..4488)
                     /locus_tag="PB780_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDE0733287.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4501..6003)
                     /locus_tag="PB780_001223"
     CDS             complement(4501..6003)
                     /locus_tag="PB780_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDE0733288.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6551..8230
                     /locus_tag="PB780_001224"
     CDS             6551..8230
                     /locus_tag="PB780_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDE0733289.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8357..8836
                     /locus_tag="PB780_001225"
     CDS             8357..8836
                     /locus_tag="PB780_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HDE0733290.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            9055..9735
                     /locus_tag="PB780_001226"
     CDS             9055..9735
                     /locus_tag="PB780_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDE0733291.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9743..11074
                     /locus_tag="PB780_001227"
     CDS             9743..11074
                     /locus_tag="PB780_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDE0733292.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            11089..12051
                     /locus_tag="PB780_001228"
     CDS             11089..12051
                     /locus_tag="PB780_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HDE0733293.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            12162..12500
                     /locus_tag="PB780_001229"
     CDS             12162..12500
                     /locus_tag="PB780_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HDE0733294.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12579..13286
                     /locus_tag="PB780_001230"
     CDS             12579..13286
                     /locus_tag="PB780_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDE0733295.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13389..14870)
                     /locus_tag="PB780_001231"
     CDS             complement(13389..14870)
                     /locus_tag="PB780_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDE0733296.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            15180..15716
                     /locus_tag="PB780_001232"
     CDS             15180..15716
                     /locus_tag="PB780_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDE0733297.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            15794..17164
                     /locus_tag="PB780_001233"
     CDS             15794..17164
                     /locus_tag="PB780_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HDE0733298.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            17231..17566
                     /locus_tag="PB780_001234"
     CDS             17231..17566
                     /locus_tag="PB780_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733299.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(17647..18504)
                     /locus_tag="PB780_001235"
     CDS             complement(17647..18504)
                     /locus_tag="PB780_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDE0733300.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            18611..19126
                     /gene="tadA"
                     /locus_tag="PB780_001236"
     CDS             18611..19126
                     /gene="tadA"
                     /locus_tag="PB780_001236"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDE0733301.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            19149..19233
                     /gene="ffs"
                     /locus_tag="PB780_001237"
     ncRNA           19149..19233
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PB780_001237"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            19443..20828
                     /locus_tag="PB780_001238"
     CDS             19443..20828
                     /locus_tag="PB780_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDE0733302.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            20865..21542
                     /locus_tag="PB780_001239"
     CDS             20865..21542
                     /locus_tag="PB780_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HDE0733303.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            21550..22314
                     /locus_tag="PB780_001240"
     CDS             21550..22314
                     /locus_tag="PB780_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HDE0733304.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            22316..22975
                     /locus_tag="PB780_001241"
     CDS             22316..22975
                     /locus_tag="PB780_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HDE0733305.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(23005..23514)
                     /locus_tag="PB780_001242"
     CDS             complement(23005..23514)
                     /locus_tag="PB780_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDE0733306.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(23626..24624)
                     /locus_tag="PB780_001243"
     CDS             complement(23626..24624)
                     /locus_tag="PB780_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDE0733307.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            24825..25409
                     /locus_tag="PB780_001244"
     CDS             24825..25409
                     /locus_tag="PB780_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDE0733308.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(25486..26679)
                     /locus_tag="PB780_001245"
     CDS             complement(25486..26679)
                     /locus_tag="PB780_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDE0733309.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            26743..27123
                     /locus_tag="PB780_001246"
     CDS             26743..27123
                     /locus_tag="PB780_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDE0733310.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            27197..27559
                     /locus_tag="PB780_001247"
     CDS             27197..27559
                     /locus_tag="PB780_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733311.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         9065 a         4686 c         5797 g         8287 t
ORIGIN      
        1 gaaggataga taatggatat ttataagtgt ttattatttt ttttgcaatg aaattcaatt
       61 tttcaatgat tgtaaaatga aaaaaacagg acgagatttt ctctaatcta aaaataaaaa
      121 aagaatttta aggaaaaaca gagaaataaa atacttaata taaatggaaa gagaaaaaaa
      181 taatcataca taggagaaaa aaaacaaaat agaaaaaagt gattttctaa taaattattc
      241 atgtggaaat gtcattttta ctgtataatt catttgatta tccatttttt attcatcaaa
      301 attactgaaa aaagaaagga agaaataaat ttgaagaaaa caaattcttc aggaaaaccg
      361 aaacgcgact tcactaaatt atcgacaccc catacgtatg tcattatttt tggtgtagtc
      421 atctttgcat ggatactgac ttttgtcgtt ccagcaggaa agttcagcac gcaagatatt
      481 gaatacaaag atgcaaatgg cgaaacaagt actcgtactg ttttacgtca agattccttc
      541 cgttatgctt acgagctgga taagtcgtat gtgtttgatc aattagaaga actgcaagat
      601 catccagcag aacgtgaaaa actggatgtt ccggaaaaag gattggaaaa agtcattgct
      661 gacggagaaa aaaatcttac gcaggaaaaa ctagatgaaa tctccttgac agatgatgtg
      721 ctttacgatc agtacggcga aaatatctat gatacttcta aaaaattgca taaaacagcc
      781 aaaatatggg gaacagatga ttttggcggc tttggtttct taaattttgt ttttgaagga
      841 ctagtatctg gagataagta tggatctgct gtagggatcg ctgcattgat ccttgttgtt
      901 ggaggtgctt ttggtattat tatgcgtacc ggagcgatcg acgcaggtat ctatgcgttt
      961 atcagtaaaa ctaagggact ggagcgttta gctttaccgc tattattctt tgctttttca
     1021 ttcggtggcg ctacattcgg gatggcagaa gaggtaattc cattctcaat ggtgatggtt
     1081 ccatttgtca ttgcgttagg atatgactcg atcgtagcag taaccgtgac ttatgtcgca
     1141 tctcaagtcg gaaatgcgac ctcttggatg agcccgttta gcgtagcggt ggctcaaggg
     1201 attgctggaa tccccgtgtt gtctggtgcg acattccgat tgattatgtg ggtagttgtt
     1261 actgcattag ccgctggtta tatgatgata tatgcagaaa aaatccgtaa aaaaccagaa
     1321 agttcgttga cttacaaatc agatgattat ttccgttctc atatcaaaaa gacatctgat
     1381 gaaaataaaa aattcatgct tgggcataaa ttgatcttat tagagatgtt agttgtcctt
     1441 gtctggatcg tttggggcgt gactcaaaaa ggctattaca ttccagaaat cgcttctcaa
     1501 ttctttgtga tgggattagt tgctggaatc attgctgttc ttttcaaatt agatggaatg
     1561 aaagtcaatg acatcgcttc ttctttccaa tctggtgcag ctgatttagc tggtacagca
     1621 atcgtagtag gtatggccaa agggattctt cttgtcttgg gaggatctga tgcatctgtt
     1681 ccttcagctt tgaatacgat tttgcatggg atcgggacgg cgttgaccgg tgttccagct
     1741 gtcattggtg cttgggcgat gtacatcttc caaagtttgt tcaacttggt tgttacatca
     1801 aattcaggac aagcagcttt gactatgcca atcatggctc cactggcaga tttagtaggt
     1861 gtatccagac aagtagcagt tcttgcttat cagcttggtt caggttttat ggacgctttt
     1921 acacccgtat cagccagctt gattggtgtg ctaggtgttg cacggatcga atgggcaaaa
     1981 tgggcaagat tccaaatcaa gatgcaagga ttcctgtttg ttttaggaac tattttcatc
     2041 atgattgcca ttgcaatagg tctgcagtag tactggagac ctgtaaataa aaaagtaatc
     2101 caaacagtag ataaggactc caagaaccgt cattttacgg taaaatcaaa ggagtaaaga
     2161 tcctatcgtt tggattattt tttaagatcc tatcatcagt tatacttaaa gaaagaggaa
     2221 aaatgtatga aaatcattcg acaaattgaa gtgtttgcac cagattactt aggaaaaatg
     2281 gatgtactga ttgccggtgg gaagattttg gctgtagaag aacagctaga aggcggctac
     2341 gaaggagtgg aagtagaaga actttcaggt gagggaaaag tactaacccc gggtttcatt
     2401 gactgtcatt tccatatttt aggtggagga ggagaaggcg gttatcaaaa tcgaacgcca
     2461 gaagtcacat tgagccaatt gactacagct ggtgtaacga cagttgtcgg ttgtttggga
     2521 acagacggag aaggtagaga tatgactgcg ttgatcagca aagcgaaagg tctggaagca
     2581 gaaggaatct ccacttatgt atacgaaggt tcgtaccgat tgccggtaaa aacagtaaca
     2641 gattctatca tcaaagactt tttgacaatt gataaaatta tcggaatcgg agaaatcgca
     2701 gtatccgatc accgttcttc tcagccaagt tttgaagagt tcgctcgtgc agtagcagat
     2761 gctcgtgtag gaggcatgtt atccggaaaa gctgggatca tcaatgtcca cttaggcgga
     2821 ggaaaaagaa agctagagct attgacacga atcgtagaag aaacagaaat tccgatcaca
     2881 caatttttgc ctactcatgc aaatcgcaca ccagaattat ttgaagcttg cgtggcattt
     2941 gccaaacgtg gtggcacgat tgacttcact gctagcgaag atcctgattt ttgggagaag
     3001 acagatggtg aagttcgctt cagcaaagca ctgaaacgat tgatcgagga agatgtttct
     3061 ttagataatt tcacgatgac ctcagatgga caaggaagct tgccttattt tgatgaaaac
     3121 aatcatttcc tagggttagg agtaggaagt gccaaggcat tgctagtagg gatcaaagaa
     3181 gctgtgcaaa aagaaagtat ccctttggaa atcgccttac gagctataac cagtaatcca
     3241 gcacgtatat tgaaattgga taaaaaagga aaaatcgaaa ttggtgctga tgcagatctg
     3301 tgtatcttgg ataaagaaac gcttgatatt gatacagtta tcgctaaagg agaaataatg
     3361 gtccaagaaa aagaagtcaa agtatggggg actttcgaaa aaagttttta atcaacagct
     3421 ctaactatct tttttagaaa acgagcgttc tgtttgtctg tatctgttgt tgatgttacc
     3481 ctgaaggtag atcaataaac aaactaagag gaggaagcac aatggcagat ttaaaaggac
     3541 gttctaaaga tatgaaagat aaagtagtcg gtgaaggaaa agaagcatac ggcaaagcaa
     3601 cagacgacaa gagcaaagaa acagaaggga aagcacaatc actaggtgct gacatcaaag
     3661 aaaaagctcg aaatttaggc gacgaagtca aagacaagtt ttctcgaaaa gacgaaaaaa
     3721 aataaaaacc acttattact aattgaatca agcaaataaa aaatccgaac catatctgcg
     3781 agacagatat ggttcggatt ttttatttga tcattatcct gttttttaaa cttcgatttt
     3841 ttgaaatttt ttatttttta gttcaaagac ttcatcacag ctttttgcta cttcaatctc
     3901 atgagtcacg atgataatac agcgattttg ttcatgggcg atttcttgga aaagtttgac
     3961 gatatcttgt gaagttgttt catctagatt tccagtaggc tcatccgcta cgattagttg
     4021 atgttcacag cagatagcac gaacgatagc gactcgctgc tgctgacctc ccgaaagttt
     4081 ggtcacctgt ttttttgcta atatttcatc aattcctaca cgagccagtc gatctaaagc
     4141 aacttgtcga taattctctt gcttggtctt agtgatttcc attgctgcta tgacgttgtc
     4201 tagagccgat aaataaggaa gcaagttgta agcttggaaa ataatcgaga catcattttt
     4261 acgataactt cttaacccga ttttgttcag tgaacgatct tgatagtata cctttccttc
     4321 ttttggtgca tctagtccag aaataagcga aagaaaagtc gtttttccag aaccacttgc
     4381 tcctaaaata gcgtacattt ttccagcttc gaaattcagt tctacatctt tataaagcgc
     4441 atcttgcgga tcttgatacc aaaatcccaa ttgttctgtc cgtaacatca aattcctcct
     4501 ttagtttaat aggatttttc ttgggtttaa tcttaatatc cctatagaag ctaaaaagac
     4561 agaaaacaga ctgattccaa atgctactcc ggctaatagt cccaattgtg caggtttgac
     4621 attgatttcc aattcgctga cttgatcaga aacagcaaat ggattattcg tggttccttg
     4681 ttgaccatcg cgttgaccgc cttgttcttg tctgttacct ggcatttgtc caccgccagg
     4741 agcttgctgg ttttgtgtcg tggtttctgt ctgttggtca agtagctgct gtcccacggc
     4801 attcgcgacg acgtttccgc taagcgaagc aactcctaat gcaagaatca tacaaatagc
     4861 cacttcggta aacaattgga gaatgatttt acttctggat tctcctaaag atagcaagac
     4921 gcctaactca tgccgacgct cccgaatggt aatcataatg atcagagtaa gtatgataat
     4981 accggctaca gccactagta aaacaacatt tttagaaaaa cttgccacgt tgtttaaagg
     5041 ttccaacatt gactgataca tggaatcatt tgattgtaag ctaaatgtat ctgtgtcgat
     5101 cagcttctca gcttctttga gaaactcatc cattttatct ggatcagata gagtgtaaat
     5161 agcagaatct atcgtgttat cgtcagatgt cccatttagt tcatttgcaa aagtgtaaga
     5221 agtgtaaagt gtattcgacg gattcatgaa gttgaatttc atacccatac tgccgcttgt
     5281 ttcgctggat tcataaattc ctttgatcgt catttcataa gtggtatctt catcttcagt
     5341 actcgtgatt gtgaaagtat ctccgactgc caggtcattt gtttctgcta gagtagaatc
     5401 aatgactaca ttgtttgtgc ctttgtcgct gtcatctatt ccttcaccat ccgtgatttt
     5461 tgctgttcca tcagaaaaat tactattttg tgcagttgct gacacaccac tgatttgaaa
     5521 gtctgcttga gtcatttgcg gattcccgcc cattcctcca gcaggttcac cgcctgcatc
     5581 agtagtttct gaactgtctt cacttgaatc agaactagtg atggcagtta ttccatcttt
     5641 tgccatagct gaggcggaac tttcaaagga ataactcttt acattatcta attcagctat
     5701 tttttccgcg tccgttaagg aaactggtgt aagagaaaaa ctgcctggat ctggacgttc
     5761 ttcagtggta gaaccgcttg aagaagtttg ttcctgtttt ttaaatgaag cttctcgatt
     5821 ggcacttaaa gtcactgttg ctccaacttc tttttgtgct tgattcgctg cttcttctgc
     5881 agcgcttcga atcgtgaggc cagctagcac aaaaataaga atggctgtga acacggcaaa
     5941 caataacaat gaacgtcccc attttacttt tgtacttttc catgcacgtt tcataaaatt
     6001 catattttgc atccctccta gtagatgcat ttgataatag acaacctatc ttaaagctag
     6061 cttaaatctt atgctgtttg cacaccatca acatgaggtt ctgtatatat aaaaagtcac
     6121 tatagttatt gcaaaaaatg tggtaaaatg aaaaaagtat gtttcaaacc gacgaagaag
     6181 aaatgaaaga ggaatagcaa aagaaatcct agatgctaca gagggatttt tacgtgcata
     6241 gttgttctaa caattgctga tactagcgat ttagtttatt tgttgtataa taatggggaa
     6301 cagctgaata cttatataag tattctggct atagcgtttc gcaaaagatc cattttgtta
     6361 atgaagtata tccaaaataa aatgcccatc caactgtcag aacgcggatg tgctggtaat
     6421 tttcctgctg attctccata agttgagaaa tagtgatttt gcatatgctt cattggaatg
     6481 gctcatatat ttagataaag aagaaaatcg acatctttaa acttggatgt cataagaaga
     6541 ggtgaagttt gtgagtacca ttaaaattat cccattaggc ggcgttcgtg aaaatggaaa
     6601 aaacatgtat gtcgcagaag tgggcgagga tatttttatt ttagattgtg ggctaaaata
     6661 tcctgaaaat gagttgttgg ggatcgacgt cgtaatccct gattttacgt atttagaaga
     6721 aaacattgat cgtgtagctg gtgtcttttt aacacatgga catgcagatg cgatcggggc
     6781 acttccttac ttgttgtcaa aaatttctgt accagtattc gggacagagt tgacgatcga
     6841 gttagcaaaa ttgaatgtca gtcgtaatga agcagccaaa ggatttaagg atttccatgt
     6901 ggtagatgag catacagaaa tagactttgg tgaaacagta gtgagctttt tccgtaccac
     6961 tcatacgata ccagattcag tggggatcaa tctgaaaaca aaagaaggta gtatcgttta
     7021 tacgggagat tttaaatttg accaaacggc tatcccaatg tatcaaacag attatgcacg
     7081 attggcggaa atcggcaaag aaggagtctt agccttacta agtgaatcgg caaatgcaga
     7141 aaatcctcag cctgtagctt ctgaagccca aatagcagat gaagtatttg atacgatcaa
     7201 atattgggaa gggcgtatca tcgtcgcttg tgtagcaagt aatttgcaac gagtgcaaca
     7261 agtattggat gcagcagaaa aagcacaacg aaaagttgtc ttgactggtc aagatttcga
     7321 acgaattatt cgcacagcga tgcgtttaga aaaacttcaa ttaccaagtg aagatctgct
     7381 tgttacgttg aaagacatga aaaactatga tccagaagaa ctgatcattt tagaaactgg
     7441 ccgaatgggt gaacccatca agtctctgca aaaaatggca aacggtacac accgtaatgt
     7501 caagattgaa gaaggagatt tggtttatat cactactacg ccaagcatcg cgatggaaac
     7561 catcgtggcg aaaacggaag atatcattta tcgagctggt ggaacagtga agttgatctc
     7621 tgagaatatg cgcgtttctg gccatgccaa tcctaatgac ttgcaattaa tgatcaattt
     7681 gatgaaacca acatattttg tgccagttca aggagagtat cgtgaattag ctgctcatgc
     7741 agatttggct catgcagtag ggatgccata caaaaatatc tatattactg gacgcggaga
     7801 cgtgctggaa tataagaaca agcgtatgag tgttgcagga actacaagtg cagaaaatgt
     7861 aatgattgat ggattaggca taggtgatat cggaaacatt gttttacgtg atcgaaaaat
     7921 cttgtcagaa gacgggattt tcgtagcggt tgtcactatc agtcggcgag aaaagaaaat
     7981 catttcaaaa cctcaaatta cttcacgggg attcgtttac gtaaaagcta gccgtgatct
     8041 gatgaaagaa agcagcaata tgattgaaac aatcgttgag aaacatttag aaagcaatga
     8101 ttttgagtgg agcaaattaa agcaagacat ccgagatcaa ttaggccgtt acttatttga
     8161 acaaacaaaa cgccgcccag tgatcttgcc agtcattatg gaagctactc aacgtcgaaa
     8221 taaaaaataa acaaaaagag taagatgaac ctctctatag agtgctcgtc ttgctctttt
     8281 tttgcgaaaa acaaataatt tttgaataca gcatccctcg ctttataata aacgatagaa
     8341 taaagggagt gaatcaatga tgagtcaaac aaaagtcgaa cgttatttag aagaacaaaa
     8401 gatcagctat caaccacttg attttagtca tgtggtagaa gaatctttca cagaagaact
     8461 acaaaaaaga gggattgatc cagctttgat atgtaaaact ctcgtagtga aaggaaacaa
     8521 aactggggtg atcatcgcat tgattccttt gaacgatcac ttagattaca aaaaaacgag
     8581 acaagtgacg aaagatcgta aagttggttt tcctggaatg gattttgtat taatgcatac
     8641 aggatatccc cacggagcaa acacccctat tggaataaaa ctcgcacatc ctgattattg
     8701 ttttttagca gacagttctt taaaagaaaa agaagaagta attgtgtcaa gcggtgaaat
     8761 cgggagaagc cttcggataa aagtagccga tttagaaaag atcgtccatc ctatttacgc
     8821 tgatttaatt agttaaggga cgagaagaga agctaagcag agcagagacc tcaaacttga
     8881 gattatatct gcttggtact tggtgcatcc tgtattatct gttacaattg ctttgtacat
     8941 actgtactat caatccaatg aaaatatatt tagcagctat attttttcat tgatgtgcaa
     9001 gtgatgaaat cgtttttaat aaaaaagata cagagaaaac gcgaggtgta aaatatgaaa
     9061 attacgtatc atggacattc ctgtttaaca attgaaatgg aaaatggcca acgcgtgatg
     9121 attgacccgt ttattacagg aaatccattg acagacttaa ctgcagaaga agtaaagaca
     9181 gattggttgc tgattactca tggtcatagt gaccacatag gagatatgat tcctattgca
     9241 aaagaaaatc aagccacaat tatcagtatt gttgagattg ctgattatgc gaacagcaga
     9301 ggagtagatt cattcggtat gaatatcggt ggaaaacatg cgtttccatt cggcgtggta
     9361 aaattcgtac atgcacagca tagttcaagc tatgaagtag acggtatcgt tcagtatatg
     9421 ggcgagccta gcggaattat tattcaagca gaaggaaaaa cgatttatca tgcaggggat
     9481 actgcttatt tcagtgatct aggattgctt gcagaagaat tcgatattga tgtcgctttc
     9541 ttgccgattg gcgataacta tacgatgggg ccagaagatg cagcaaaggc tgcgcaaaca
     9601 attcgtgcca agaaagtagt tccaatccat tacaatactt tccctgtcat tgaacaagat
     9661 cccgaatctt ttgtagagtt actggaagat agtgaaggaa aaataatgag cgtctcggaa
     9721 acgctggaag tgtaggcaga aaatggcgac aaaacatgat ttgattttag aatatattga
     9781 aagtttacct gttggcaatc gaatttctgt aagaagcatc gcaaaagaat tgaatgtcag
     9841 tgaaggaaca gcgtaccgtg caatcaaaga tgcagaaaat gtcggactag tttcaacaat
     9901 tcaacgagta gggaccatcc gtatcgaacg caaattaaaa aaacatattg aacgtttgac
     9961 atttggcgag gtcgtcagga tcattgaagg ggacgtttta ggcggggcgg caggattaga
    10021 caaggtcttg aataaattcg tcatcggcgc gatgactaaa catgccatgg aacgctatat
    10081 tacgccagga tcactaatga tcgtcggcaa ccgggcggaa gtccaaaaat tagcattgga
    10141 agatggcgcc gcagtattga tcacaggagg attcaatacg actgctgaga ttgcaaagct
    10201 agctgatgat cttgaaatgc cgatcttacg cacctcttat gatacattta cagtcgcaac
    10261 catgatcaac cgagcgttaa gcgaccaatt gatcaaaaaa gatatcatgc ttgtcagtga
    10321 tatctatatg ccagctgaaa agactcatta tttgcagcag acagatacga tttctgatta
    10381 tcagcgtttg tcagaagaaa cgcagcattc gcgttttcca gtcgtcaacc gccatcatcg
    10441 tttgatgggg attgttacgg caaaagatgt tttagggaaa tcgccaaacc agatcatcga
    10501 tcgggtgatg acgaaagaac caatcagtgt aaagaaaaca atgagtattg cctctgttag
    10561 ccatcaaatg atctgggatg ggctagaagt catgccggtc gtttccgatg acttgacatt
    10621 ggaaggcttg attacccggc aagatgtaat gaaagcgatg cagcttgtcc agcgacaacc
    10681 gcagattgca gatacgattt cggatcagat atctggaaat atccatacga ttgatactga
    10741 ccgtgaagga aatcgtttgg aaaaccctaa gttcaaattc gtagtagcac cacaaatggt
    10801 caatggcgtt ggaaccattt cttttggcgt acttagcgaa atcatttctg atgtcgcaca
    10861 aaaaacaatg gtgatgaacc aaaaacgaaa tatcttgatt gaacaagtga acctacatta
    10921 tttgcggctg atccaattgg aaagcgaatt agacattcgt ccacgtgtat tggaaatcgg
    10981 cagacgttcc gctaaactgg atatcgaagt gtttattgaa aacaccatcg tttccaaagc
    11041 aatcgttgtt tgtcaagtaa tggaacgttc ttagaaagca ggagtgaaat gacagtacag
    11101 caagaaatag tagatacaat aaaaaaatac gataaaatca ttatccacag gcatttgcgc
    11161 cctgatcctg atgcattagg atcacaagta ggattagccg aactattacg ggaaagtttc
    11221 ccagaaaaag aaatacgtca agtaggtgaa acgattgaag ggcttcgctt tttagcagaa
    11281 atgcaagaag ttgaagatgt cttctaccaa ggagcgctag tcatcgtgac tgatacagct
    11341 aattcaccca gaatcagtga tcaaagatat cagttggcag aaaaactgat caaaatcgac
    11401 catcatccca atgatgaccc ttatggagat cttgtgtggg taaatacaaa agcaagtagc
    11461 tgtagtgaga tgattgcaga atttgctttg atgtttcctg aatctttaaa gatgaacgtt
    11521 aaggctgcac ggttacttta tgcaggaatc gtcggagata cagggcgctt tttgtatccc
    11581 tccacgacgt cccgtacatt agagattgct tcgatattga gaaattatcc atttgatgca
    11641 agtgctttga acagagagat tgaacaaatg ccgatgaaag tcgctaaact ttccggttat
    11701 ctttatcaaa atatccaggt ggatgaaaat ggtgcaggaa gaataatcct tccgcaagaa
    11761 attcttgata aatatggaat cgatgattca gagacagctt cgatcgtttc acttcctgga
    11821 gtgattgacg aagtgctggc ttgggggatt tttgttcagc agccttcagg gtattaccgc
    11881 gtacgtttac gttctaaggg gccgatcatc aatgaattgg ctaaacgcca tcatggagga
    11941 ggccatccat tagcaagtgg tgcgaatgca aaagatttag cagaagtaga ggaaatctat
    12001 aaagagatcc aagatatatg ccaatcgtat aagagcagaa aaacagaatg atacttcgtt
    12061 cgaaaacgtg ccttttgtag taaaaatcac aaatgtatct tttcaagatc ttctttttcc
    12121 gttacaatag tgctcactaa agattttaaa ggagaaagaa gatgacatta ccaaattgcc
    12181 cagaatgtgg ctcagagtac acatatgaag atcgaggctt gttgatttgc ccagaatgcg
    12241 ggaacgaatg gaacaaagaa gaacaagaaa ctgtcataga agacggatta gtggtattag
    12301 atgcaaatgg aaaccgtctg ttagagggcg atagcgtgac agtgattaaa gacttgaaag
    12361 taaaaggtgc gtcaggtgcc attaaacaag gaacaaaagt caagaacatc cgtttagtag
    12421 aaggcgatca taatatcgat tgcaaggtag aaggatttgg accgatgaaa ctgaaatcgg
    12481 aatttgtgaa aaagaattaa agaatattca aggtcttttc acattctttt cctactacta
    12541 atgataaaat aaagtaaacg aaaaggagtg aaacgaaaat gtatggttat ggaatgtatg
    12601 gttttttaga tccgacattt cttttggtca ttcttggatt agttatttcc ggaattgcgt
    12661 cagcgtacgt caatagtact ttccgaaaat acgatcaagt gagaagcaaa aacaatgtga
    12721 ctggtacaca agcagcacaa tatatattac aaagtcaaca gataaataat gtcggtgttc
    12781 aacaaattgc cggagatctc acagataact acaattcagg taataagatg ttgagtttgt
    12841 ctcaagcaac tgcacagtct acttcagttg ctgcgatagg tgtagcagct catgaatgcg
    12901 gacatgcggt acaagatgct tcgggttatg tcccattgaa gattcgtgca gcacttgttc
    12961 cagcagctaa ctttggttcg atgatttcgt tccccttgat tatggtaggt gtattgctta
    13021 gctggaactc tacgttaatc aatatcggga ttttcgcttt ttctttagca ttacttttcc
    13081 aattggtgac attaccagtc gaatttaacg cgtctcggcg agcaatccaa attttgagtg
    13141 aaggcggact actgacggaa gaagaagtac cgatggcaag acacgtatta tttgcagcag
    13201 cactgactta tgttgcagca gcgctttcga cattcctgca gctgttgcgc ttgatcttat
    13261 tgttcggcgg caaccgaaga gattaaaata aagaagttgt gaaatcagcg ctttgatctg
    13321 agctaatacc gagagttagc tgattgaata ccatttattt ggaataagag gatgtgacaa
    13381 gaattttgtc acatcctctt tgttttgttc tcataacctt ttttcatata cagttcatga
    13441 accgctttta ttaacttgcc aaataacact gtattgtgcg ttgtatccag atagaaagtc
    13501 tccgtttcat ctgaaatttt ctcatgctga tctgtaatga tcaaatcagc atgttctgaa
    13561 tatgcggtaa ttttaacagc atctttgcga aataactgct gcaaatggct ttgaagatga
    13621 taatttcctg taaactgttt ggttatctgc aaatggatga gtaactgctc tttcgtttct
    13681 gtttgtagta agtgttggat caattgacta aggtattgac tgatttggtt tctttcccca
    13741 ttttttgaaa aaattgtatc gattttttct cttaatttct tttcctgtat aaaagaacta
    13801 gaatgattgg acgtggcttc tggaaagcaa agtgtagaaa aactactgct ggattcaccc
    13861 aacagttcga aaaaaacaac tgccaacaca agaaaataaa aataaattgg tattttattt
    13921 tctaaatggt tgagaggaaa ctgttgagtt aagtaagtca tcaattgacc gacttgtcga
    13981 caaataaggt ttttgcttgt ggaaaataaa aaaccaatct cctgtttttg tcgaaaagag
    14041 atttgatttg gaaaaaaaca atagaaaaaa taattaaagt aggctctctc tgtttctgtc
    14101 tcttcacaag ggcgactcac tgaaaaagaa gtaaagtatt ctgaataatc atgtgtatgc
    14161 tgcaataagt ccatcaattc agtgatttca gaagaagttt ttggaaaata tgcctgttgt
    14221 ttgtttcgta atatgaataa agttaacagc aagtagatat ttttttcttg ttttgaaatt
    14281 ttttctattt gctcatctgt actgttcatt tttagtgcat ttaattcaag tgaggaaaat
    14341 ggccaatcgt tacctccaaa tgtatctgaa agaaaaaagt aaagatagag acggatagat
    14401 aactcatttc cttcaagagt acagcaattt tttacagata ggatccgtaa atggaatttt
    14461 tttaaggaat gattgagctg ttttgtcagt ttttcgagat aggagggcga tatataaagt
    14521 tgttcaagta aatatttttt tggtacagca gtttgtggat aattgattag taacatcaaa
    14581 tagttaaaac gattggattc ttttaggtag cataatttta acttagaaaa aacagctaac
    14641 tttttagttt gatcggtttc tttggtttgg atctctcctt tatgattaga ataaataaat
    14701 tcatcaatca ataaaaattg cttgagtgat acatttattt ttcggatcag ttctttgatt
    14761 gtccgttttg gaatatgtaa gtcattggat aattcttgta aagaacaatt atttttttga
    14821 atgaccattt gtagaacaag taactctttt tttcttgctt caattagcat aaatacctcc
    14881 tttttattct gtttttgtat atagttagac aaaaactgtg agtggaaagt catttcagtc
    14941 tgtgttattc tcacagagtg tcgccttctt ttttatataa cgttatttaa ttttttttgt
    15001 taccttgttt attctattgt ttatttccta gggtattttg tcaagatatt cttgttttta
    15061 aggcggaaaa attgattttt aatggatata actaaatatg ttttgataat tatcaataat
    15121 atttttagaa ttatttatgt tatttattta tgggtagatg aaaagagacg gaggtaagaa
    15181 tgatggggag agtaacaaat tatagtaaag aatatttgct gttcaaaagt atggtatacg
    15241 tggaagaata tggaatgaag tcaattactg caagagagtt agcagagttc tgcggttgtt
    15301 ctacataccc gatttataca catttcaaat caatcagcgg gttaaaagag aagatattag
    15361 aagaaatcac tgtttatttt gaaaactact tagctgaatg cagttccgat gatgttttaa
    15421 gcattgtcta cttattgaaa gatttttttt ctatccatga aagtattcgt gaaatcattg
    15481 caaaatctga tttagacatg gattcgctat ttaagcggcc atttttctca tatgtcaaag
    15541 gtcacacgaa gatcaaagaa gaaaagtcgc ttgaattggt atggcttaat gcattgggaa
    15601 gtttgcgctc agatgattct tctcaagatt ttcttgaaat gatgaacgct gtttgggata
    15661 aaaacgaggt gacggatgaa tacttggaaa atatcttaaa ggaacagtca ccttaggcag
    15721 caacatgaaa aagtagatca gccaccttta tttttcgtaa aaaagattgt aaaatgtcta
    15781 acgaaacatt ggaatgaagg aggaagcgac aacgttacca cagcaattta tcaaaaaata
    15841 tcgtcttctt ttaggcgaag aagcatccga ttttttttct gcgcttgaac aaggaagcgt
    15901 gaaaaaagga tttcggtgga atccactaaa accggcagga ttggacatgg tccaaactta
    15961 ccatagcgaa gaattacaac ccgctcccta cagcaatgag ggatttcttg gcactgtcaa
    16021 tggaaagtct ttcctccatc aagctgggta tgaatatagc caagaaccaa gtgcaatgat
    16081 cgttggaaca gctgctgcag caaaacccgg tgaaaaagtt ttggatttgt gtgcggctcc
    16141 cggtggtaaa tcaacccaat tagctgctca aatgaagggg aaagggttat tagtgacgaa
    16201 tgagatcttt ccgaaacgtg cgaagatttt atcagaaaat atagaacgat ggggagtttc
    16261 gaacgcaata gtgacaaatc atgcacccgc agaacttgtc cctcattttt cgggcttttt
    16321 tgatcggatc gtggttgatg ctccttgttc aggagaagga atgttccgaa aagatccaaa
    16381 tgcaataaaa gagtggacag aagaatctcc gctatactgt caaaaacgtc agcaagagat
    16441 tttgtcttca gcaataaaaa tgctaaaaaa taaaggacaa ctgatttatt cgacttgtac
    16501 gtttgcacca gaagaaaatg aagagatcat tagctggctt gtagaaaatt atccagtaac
    16561 gattgaggag attcctttga ctcaatccgt tagttcagga cgttctgaat ggggaagcgt
    16621 agctggtttg gaaaaaacaa tacgtatatg gcctcataaa gatcagggtg aaggacattt
    16681 tgttgcaaag ctgacttttc atggacaaaa tcagatgcat aaagaaaaga agacacgaaa
    16741 aaaaagtaaa gtgcaaatga caaaagagca ggaaaaactc tggacggagt tttcaaatga
    16801 ttttcattat gaagctactg gaagactttt agtgttcaac gaccatcttt gggaagttcc
    16861 tgagttagcc ccttctctcg atggtttaaa agtcgtacgt acaggtttgc atttaggaga
    16921 tttcaaaaag aatcgatttg aacctagcta tgcattagcg cttgctacaa aaaagataga
    16981 gaatatacct tgcttgccga tcactcaaaa ggaatggcaa tcttacacag caggagaaac
    17041 atttcaacgt gatgggaatc aaggatgggt tttgctagta ttagataaga tacctgtcgg
    17101 atttgggaaa caagtaaaag gaacagtaaa gaactttttc cctaaaggat tgagatttca
    17161 ctgagtggat tagttcgttt atttttttgt ctatgctatg atggagatag ataaaaagga
    17221 gctgatactt atgaaaacat caacaaaagt aacaatcgga ttaagcgtag cagccgctgc
    17281 cagtgtagcg acagcagtcg ttgtatcggg aaaagtaatg gaaaagatcc atcatatgac
    17341 tactcggacg aaagtaaaaa aattcgtcta tgacaaattt gatggaaatg aaaaattact
    17401 agaaatcgta gatggtcttt cagatagtga cttggattct ttgatgggaa tgttagcaaa
    17461 aatcaaatca ggaaaaaaga aaatctctgt ttatggtgat tcaatcaaag attcaaccga
    17521 agatgtaaaa gaccggctaa tgcattttgt tgaaaaaatg atgtaagaga aactagcttt
    17581 tgaacactat tcattcgaaa taaggggttg tgacaaaatt attgtcacaa cccctttaat
    17641 ctatctttaa ttatgttcat gaagcaataa aagatagcaa aacagtaaat cgttggcttt
    17701 ttttagattg aaaccagact gttcttgaaa tttttcgatt cgatatttta gagtattacg
    17761 atgaagaaac agtgtttttg cagcagagct gacattgcct aattcattcc ataattcttg
    17821 aatgatcact tccatttcat tttgtttgat cagttctatg taacttttga tcaacttact
    17881 atcttttaca gtatcttttg tatagtaatg taaagccaca tcttgcatag aaaaagtacg
    17941 gctgtgagta taaagattgt tttgctctgt taaaaaaatt tttctttctt cagcaaacaa
    18001 agggacgagg tcggtgccag gtgaatggaa agaaccaacg aatacagacg ttgtcgtatc
    18061 aaaatcagca tctaaagata gaaaaatacc gttgatttca gtagcatcaa gataatcacg
    18121 gtttatttgt tcgactaaga gcgcatctgt gtctgtataa aaaaagaaat cagcagaata
    18181 gcgaaataat tccaaaatag tatcttgcca ttcttttttt aagaattctc cttttgattc
    18241 gacgtgaagc tggatcaaac gaaagctttt ttcttgaaca caagaagcat ctttaaaaag
    18301 atagtgatac catgggtgtt ttttttgatc gttgattaca gggaataacg ctttcaaaag
    18361 gctggtttct tgttgagata atgaatcttg tttgatccag ataaaagcat catcaattgc
    18421 aaaagaaagg atatccgctt ctgatgctgg agagtttttt ttctgtgctt gtgggtaaag
    18481 agcaagtaat ttatccattt tcatcttgtt cacctcttgt atttatttta ccatagaaaa
    18541 aaaagttagt tacagccttt ttatcatatg ctaaagtaaa acaagaaaaa acaaaggcag
    18601 ggaagggtaa ttggtaaata caaatcttga tgcagtagaa aaagaaaaat ggatgcgcct
    18661 tgcattggca gaagcaaaga aagcagaaat gcttcatgaa gttccgattg gagcagtggt
    18721 tgtattggat ggaaaagtga ttggaagagg atataacctt agagaaacca cgcaagatgc
    18781 tactacacat gcagaaatgc ttgcaatcaa ggaagcctgt gaaaaagtgg gaagctggcg
    18841 cttggaagat gctgcacttt ttgttacctt agagccttgt cctatgtgca gcggcgcgat
    18901 gattttgtct cgtgtagcag aagtttattt cggcgcgtat gatccaaaag gcggaacagc
    18961 cggtaccttg atgaatcttt tggaggacga gcggtttaac caccaagcat atgtagaagg
    19021 tggcatttta gaagacgaat gtgggatgat tttgactgac tttttccgaa agctccgcca
    19081 gcgaaaaaag aaattaaaaa aagactggtc aacgaatgaa aaataaggta tactattctt
    19141 tgccgaaagg ctaggacaat ggtgggcttg tgaagcgtgt caggtctgga aggaagcagc
    19201 actaagcagg ttccgccatg tgtctgatta taatgaaacg acaacagact ttcgggtctg
    19261 ttttttttat ttttatagta ctgtaaaaat gaatgttcct gattttttag ccagactgat
    19321 ttatcgaata ttgtacttat acaatttgct tcaaggcagg aaaaactctg aaatagagct
    19381 tgtttcaatg gaagaaaaag ctgcctatgg tatgataaag ataaagagag gcgagagggt
    19441 acatggaaaa aatagtgatt ttacatacga atgatctgca ttctcattta gaaaattggc
    19501 caaggatcag aaggtttttg gatcaacgta aacgagaaaa cgaaaagaaa gaaaaaacga
    19561 caacgattgc agtagatcta ggtgattttg tggatcgttg gcatccattg tcagaagcaa
    19621 cgaatggaca ggaaaatatc ttgctgatga atgaagttgg ttatgatgca gcgacgatag
    19681 gaaacaatga aggcgtagga aattccaaag aagagttgaa tcatttatac gatcatgctg
    19741 agtttgatat catcctggat aacttattcg ataaaaatac gctccagcca ccggcatgga
    19801 cgaaaccata taaaatcatt acaacgaaac accagacgaa aatcggacta attgcattta
    19861 ctgctccatt tccgcttact tataatccga atggctggga catacggaat ccttatgata
    19921 ttttgccaga attagtcgaa gatttacgtt cacaagtgga tgttttagtt ttgatgagtc
    19981 atctcgggat tcaggatgat ttccaaattg caaaagaaat accagcaatt gatgttattt
    20041 taggatcgca cacccatcat ttattcagag aaggtcacgt aatcaatgat gtccaaatag
    20101 cagccgctgg aaagtacggg cagtatgtcg gtgaagtcca tttaaccatc gatgaagaga
    20161 aaaaaatcct taagcattca gccaaagcca tccctaccga aacaatgaca gcttttacag
    20221 aagatgaaca agaaataaat agttatctag aacgtgggca tgaattatta aaagaaaaag
    20281 aagtagcaga tttgccattt tctctgagtc tagatatctt caatgagcat tcgtttatcc
    20341 aagtagcctt tgaggcggtg aaagaacgag gaaagacgga agcagcaata ttaaacagtg
    20401 gtctgttttt aactgaaatt ccaaaagggc gggtcaatca ggatcagctt cacactgcgt
    20461 tgccgcatcc gatgcacttg ttaaatgtca cattgggtgg aaatgatctg atccgcttag
    20521 ttttagaaat agaaaaaaat cgtaactttt tacgtaacta tcctatgaag ggaatgggat
    20581 ttcgcggtaa gatttttggg cagatggtct ataatggaat cacatatgat tctattaacc
    20641 atcaagtctt ttggaagaac aagccaatcg atctgaataa aaattatact tttactaccg
    20701 tagatcattt gatgtttgtt ccattttttc caacgattga gattgctgga aaaaatgaat
    20761 ttttgttccc tgaatttatc cgaagtgtgg taggagatta cttgaaagct cactacccaa
    20821 tcaaataaaa acaaggtata ataaaaaaga taggtggtga acgcatgtct gcaaaacaaa
    20881 atgaacagca gaacgttgag cataaaacag aaattgctaa gaaaaaatca tcagctgaac
    20941 aggaaacaac agtcaccgaa gaactgacag ctgctttgga agaaatcgtt gaggagccta
    21001 aagcagaaaa agtcaaaggc gagctagtga cactgttaga tgacagtaat tttttgatag
    21061 gtgaaagaca ttatcggctt gtgtccgatt atcgtgaagg atttaatgca gaaaagcttg
    21121 gagaacgtta cagtgatgta ctagcccgat atgattatat cgtaggagat tggggttatg
    21181 aacaactacg cctaaaagga tttttccaag cggataatcg acgggcgcat ccagatcagc
    21241 gaatcgattc cttggaagac tatctttacg aatattgcaa ttttggttgt gcgtattttg
    21301 tcatcgagag aatcggcggt aaaaaagaaa agacaactca gcgccgaaaa aagaaaaaaa
    21361 accataatcg tgcgtatatc gatgagaaaa aaggaccagt cgcatctaat cgcaaaaaac
    21421 cggttattaa aaatcgaaaa acagagtcag caaaaacctc tgaaaaagta aaaaaacaag
    21481 aacaacctaa acaagtagaa aaaacaaaaa cagcatttac gatcagaaaa agggaagagt
    21541 agtggatgaa tggactataa aggatattta attgatttag acggaacgat ttatcgtggg
    21601 acacaaccca taccggccgg taaacgattt gtggaagaat tgcaaaaaag aaaattacct
    21661 ttcttgtttg ttaccaataa cacgactaag tcaccagaaa cagtagctaa tcgtctagca
    21721 gatgagtttg acattcatgt agctccagag acagtctaca cagctacttt ggcaacaatc
    21781 gattttatga aagcagatgg aaaagggaaa aaagtctatg ttatcggaga agcaggattg
    21841 attgacttga ttttagctgc cggatttaca tgggaagaag aggctcctga ctatgtagtg
    21901 gtaggcttag ataattatct gacctatgaa aaagtagtta aagcaacact tgctattcaa
    21961 aaaggtgcta ctttcattgg aacgaaccca gataagaata tacctactga acgcggcctt
    22021 cttcctggag cagggtcagt catctctttt gtagagacag caacccaaac gccgccgatc
    22081 tacatcggaa aaccagaagc catcattatg gataaagccg ttgaagtatt agggctgcaa
    22141 aaagaagaag taatcatggt tggagataac tatgaaacag atatccaagc tgggatacgc
    22201 aataatatag atactttgct cgttttatca ggttttacaa aagaagaaga tgtacctggg
    22261 ttgcctatcc cagctaccta tgtgaaacaa tcactggatg aatggagctt ctaatatgaa
    22321 aaattcgaga tggcagtgga tggaatatgc tggattgttc agcctatttc tgacactgat
    22381 cagtctggca gttggtgtga cgatcaactt tcgaccacta tacgtatttg atatcggaca
    22441 tttgcaaatt ttggattata cttctttaga tcaagagacg ttactgaaaa attttgatca
    22501 cctaatgaat tatctcaata atcccttcaa aaccatcctc agcttgccag atttcccagt
    22561 ttctgctagc ggagcccatc acttttatga agtgaaaatt ttgtttttag tggattatgc
    22621 tgtgtttttc atcactttga tcccgagtat tttgtttatc aaatatttgc aaaagaatga
    22681 tcggctttgg cggttgattc gtccgtttca aatcggcatg ttactgccag ttgttttcgg
    22741 ttttttcatg atgataggat ttgatcgctt ttttatcctt tttcatgaaa cgtttttcaa
    22801 caacgatgac tggttattcg atcccgttac tgatccaatc atcaatgtac ttccagaaga
    22861 atttttcatg catagcttta tcttattttt tgtattatta gaattatttt ttgctgtttt
    22921 tcttttctta ggaaaaaata gcttgaaaca aacaaaaaag aaagaactcg tctaaataga
    22981 cgttttcttt ctttttttgt tgaattattg agtaaggcga cttctttttt ctggttcttt
    23041 gtgtgctaat cgactggcag cagcagcggc gatcgctccg acgatatcat ctaagaaggt
    23101 atggacatga acgccatcgt ggctgtttag ttcttttaaa ataccaggtt tcaccttgtc
    23161 gatataacca taattcgtaa agccgatcgt accgtaaaca ttcacgattg acaaggctag
    23221 gatctcatcg ataccgtaaa ggccttcgtc atcttcgata atatgttgca acggactcat
    23281 caattttttc ttttctgcta atatatctaa ttggattcca gtgataatgg cgttatgtac
    23341 ttcacgcttt gttagcacgc tgttgacact ttcagtacaa tccgccaaag tcaaatcaga
    23401 aatatagtct ttttgtaaaa acatgacaag ttgtgcgata tcttctacag ttacgccacg
    23461 ttccttcaat agttctcttg ccttttcttc taaagtctca gttttgacga ccatattatt
    23521 gctccccctt tattttttct ttctctattt catgttaacg gaacatgaaa aaaatgcaag
    23581 aaactgcttt cgggtttaac ttgaaatgca aagagatata aaaaatcaac taggacgaag
    23641 atttttatcg tataagcttg tgctgtgacc aggctgagtt cgttcctttg gattaatata
    23701 gtgtaaagcg ttgttcactg cagtaggtgc ttcaccgagc ccagtagcaa tcagtttcac
    23761 ttttccttca taggtacaga tgtcaccagc tgcataaacg cctggaatag aagtagacat
    23821 atctgatttt acagtaatgg catttctggt actatcaagt ccccaagaag aaagatgttc
    23881 taaattagaa gagaatccat aattgacgat cagagaatca atcatcaaat caatcgtctc
    23941 gtcacttttc acttttttta accggatatc tgtcagttcc ccattattgc ctgataatcc
    24001 atcgatgaga tagggagtaa gcaagttgac agatgaagat ttcaagcgtg atacactatg
    24061 ttcgtgcgca cgaaattccg ggcgacgatg aatcaagtaa acttcgcttg caataggctc
    24121 taacatcaac gtccaatcga tagcagaatc accacctcca gcaatagcga ctttttttcc
    24181 agcatacttc atcaaatcat tcacgaaata atccaatccg tggttttcaa atgattcagc
    24241 attatctaaa ttcaattttc ttggctggaa tgaaccattt cctaaagcta aaatgactgc
    24301 tttggaataa tggatgcctt tatttgtagt aatctcgata acttcgtctt cacgagtaag
    24361 cgagagaact tcttctttta aatggaatgt atggttaaaa gtagttaatt gtttttctaa
    24421 ttgatcgatt aattcacttg cttttattgc tggataccca ggaatatcgt aaatatattt
    24481 ttcgggatac aaagttgcta attgaccacc aagttgaggt agactgtcaa tgatttttgt
    24541 tttagcttgg cgcattcctg cgtaaaaagc ggcaaacatc cctacaggac ctgcaccgat
    24601 gattgtaatg tcatagattt ccataaaaat tcctcccttt tcacactata gcagaattta
    24661 attgagaatg cttctcaaaa atgtttgaat tcgaaatcga aacggtaagt tatagaatgt
    24721 atctagtgat gaaaaataaa agaaaacaaa tctagtattt ttacgagagg ttaggtatta
    24781 tgttattatt attatgaagt atgcaaatat taggaggaaa caatatggca ttcccacaac
    24841 tagatttaaa aagtgaaaaa ggaccaaaag ctgttatcaa aacaaaccgt ggtgacatta
    24901 ctgttcaatt attccctgaa ctagcaccga agacggttca aaatttcatt gaactttcca
    24961 aaaaaggtta ttatgatggc gtgatttttc accgcgttat tcctgatttc atgatccaag
    25021 gcggcgatcc aactggaaca ggaatgggtg gcgaaagtat ttatggcgaa agttttgaag
    25081 atgaattcag tcgtgaactg ttcaaccttc gcggagcttt atctatggca aattcaggtc
    25141 caaacacaaa cggaagccaa ttctttattg taaataacac aaatgtgcca gctaatatgc
    25201 ttggacaact agaaggtgct ggattcccat cagaaatcat tgaagcgtat aaaggtggcg
    25261 gaactccttg gctagatttc cgacatacag tctttggaca tgtactagaa gggatggaca
    25321 cagtagatga aatcgcaaat gtacaacgtg gtccacaaga ccgacctgta catgatgtag
    25381 tgattgaaac aattgaaatt tcagaatagt ttggtatggt acggagtaat tatgaaaaag
    25441 gacgaaacct agtaaggtct cgtcctttta ttttgaataa atgatttaat cgattgtctc
    25501 taaagcatac ttgattcgat taagtccttc cagtaaagtt tcttctggac aagcaatatt
    25561 caagcgcata tgcccatgac cggaaggacc aaacgtgatg ccaggattta atacgacttt
    25621 tcctttccga acaagagtgt tctccaattc ttggtcgtct gaaatatagg cggtaaaatc
    25681 taaccacatt aagtaagttc cttcagggtt cattattctc actttcggaa gatactgttc
    25741 aaaaaactga ttaactgttt gaacattttt ttctagataa ggaatcagtt ggcttaacca
    25801 ttctccaccc gtctcataag ccgcttgggt tccaatcagt ccaaaagtgt tgatttcgtg
    25861 ctgttggttc atcactaaat gtttttcaaa atcagctttc atctctggat ttttgataaa
    25921 taccattgaa tttttgattg cagctagatt gaaagtcttg gttgcggaag taaaagcaac
    25981 tagtagatca tctaaattag gatcaatcgt tagcatggaa gtaaatgtat gatcgaataa
    26041 agttaaatct tgatggatct cgtcactaaa cagaatcact tgatgtttta aacacaattc
    26101 gcttaactgt ttcaattctt ctttgctcca aacacgccct ccaggattgt gaggattgca
    26161 taaaatcatt gcttttacat tgttttcttc gatttttttc tccatgtcag acaagtccat
    26221 gatgaaatga ttattttttt ctagcaaaga gcttcgaacg atgtgacgtt gatttgtttc
    26281 tataatagag gaaaatggag gataaactgg gtcatgaatc aaaatagaat cgcctggttt
    26341 tgtaaaagtt tgtactgctg cagctaaact ggttaacaca ccacttgtaa aaacgatatt
    26401 ttctttcgtc aactccacag catggtgttg gcgttcccaa tgaatgatag cctgatatag
    26461 tttatcagac agagtggcat aaccaaagat tccatgtttg atgtattcac taaacgcttt
    26521 agctacccca ccgggagaca agaagtccat atctgctacc cacaaaggaa gtaagtcatc
    26581 catttgatat gtctcagcaa ttgcgtccca ttttacactt tctgtatctt ttcttgaaat
    26641 ctgtttatca aattcgatca tcttaagacc tccagtcatt ttcgtcgctt ctttttttga
    26701 gctatttaca gtataatgat aaatgcgaag agagggaagt agatgactta taaaattgga
    26761 caaataattg aaggaaaagt gacagggata cagccttatg gcgcattcgt ttctcttgat
    26821 aacgagacgc aaggacttat tcatgtttca gaagtacaat ccggttatac gaaaaatatc
    26881 cattcattgt taaaagtcgg acagccagta acagttcaga tcatcgacat tgatgaatat
    26941 tccaaaaaaa tcagtttatc tttaagaacg ttagaaaaaa caaatccagc tgttccatat
    27001 cgcagaaaaa ggtatttcac gaataaaaat cgtaagatcg gatttaccac tattgcggag
    27061 cagttaccta tttggataga agaagcatta tctgaactag aacggaaaga aaataagaaa
    27121 taaaaagtgt attttttctg aaaatgtgct agaattttta tgtaagccat agaacaagtt
    27181 gatcgaggtg gaaattttgg caagcaaaag agttgctggt acaattatgt tgcatttgga
    27241 ggatggtact aaaaaatttt tggtccattc aattgatgac aagttggaat ttgcgttagc
    27301 ggaactttct gaaggaaaga ctggtttagc gaatatactt aatttcttaa aagaaatagt
    27361 tcatatcgat gttagcaaga ttagtttgat ggaattgaca aatacacata ttaaagagga
    27421 aaatgttccg ttgtttgtct ttgaaactga acaaaagaat caaagagaag aattaggcga
    27481 aggatatatt tgggaagaac caggtcaaat gcgctctgtt cttggaactt acgaagtaga
    27541 gggagtgcct ttcttctaaa atacagatta agcgaacaaa gaagtttgaa tttttttcaa
    27601 acttcttttt ttattgtttg gtccaaaaaa tacaatgaaa tgttctgcta aagatagttt
    27661 tttagtgcat tttattttca aaatatgttg aaaacggttg tataacgaat aataataatt
    27721 gatcgaaaaa tcatttcaca aagtaatgct taatttatag ttgaccaagg aagaaataca
    27781 tgataaattt aacagcgttg attctgaagt ggatgttata tcacatttct aaaaa
//
LOCUS       DAMEET010000033        27809 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.033, whole genome shotgun sequence.
ACCESSION   DAMEET010000033 DAMEET010000000
VERSION     DAMEET010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 27809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.033"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            108..968
                     /gene="ylqF"
                     /locus_tag="PB780_001248"
     CDS             108..968
                     /gene="ylqF"
                     /locus_tag="PB780_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HDE0733312.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            961..1728
                     /locus_tag="PB780_001249"
     CDS             961..1728
                     /locus_tag="PB780_001249"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDE0733313.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            1784..2641
                     /gene="dprA"
                     /locus_tag="PB780_001250"
     CDS             1784..2641
                     /gene="dprA"
                     /locus_tag="PB780_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDE0733314.1"
                     /translation="MYQIEENLLKLAYAKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITTHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVVEAGYVIVSGCAKGIDTYGQQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHRFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            2763..4841
                     /gene="topA"
                     /locus_tag="PB780_001251"
     CDS             2763..4841
                     /gene="topA"
                     /locus_tag="PB780_001251"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDE0733315.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDIVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            4810..6186
                     /gene="trmFO"
                     /locus_tag="PB780_001252"
     CDS             4810..6186
                     /gene="trmFO"
                     /locus_tag="PB780_001252"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HDE0733316.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLDSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            6384..7289
                     /gene="xerC"
                     /locus_tag="PB780_001253"
     CDS             6384..7289
                     /gene="xerC"
                     /locus_tag="PB780_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDE0733317.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            7326..7874
                     /gene="hslV"
                     /locus_tag="PB780_001254"
     CDS             7326..7874
                     /gene="hslV"
                     /locus_tag="PB780_001254"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HDE0733318.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            7888..9288
                     /gene="hslU"
                     /locus_tag="PB780_001255"
     CDS             7888..9288
                     /gene="hslU"
                     /locus_tag="PB780_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HDE0733319.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALIKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            9306..10097
                     /gene="codY"
                     /locus_tag="PB780_001256"
     CDS             9306..10097
                     /gene="codY"
                     /locus_tag="PB780_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HDE0733320.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            10179..11054
                     /locus_tag="PB780_001257"
     CDS             10179..11054
                     /locus_tag="PB780_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDE0733321.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNREIFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADAVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(11186..11824)
                     /gene="plsY"
                     /locus_tag="PB780_001258"
     CDS             complement(11186..11824)
                     /gene="plsY"
                     /locus_tag="PB780_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDE0733322.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            11951..12385
                     /locus_tag="PB780_001259"
     CDS             11951..12385
                     /locus_tag="PB780_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDE0733323.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEVADDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            12551..14596
                     /gene="parE"
                     /locus_tag="PB780_001260"
     CDS             12551..14596
                     /gene="parE"
                     /locus_tag="PB780_001260"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDE0733324.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLTVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEVPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            14608..17058
                     /gene="parC"
                     /locus_tag="PB780_001261"
     CDS             14608..17058
                     /gene="parC"
                     /locus_tag="PB780_001261"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDE0733325.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     VTIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            17335..19566
                     /gene="pflB"
                     /locus_tag="PB780_001262"
     CDS             17335..19566
                     /gene="pflB"
                     /locus_tag="PB780_001262"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDE0733326.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            19810..20571
                     /gene="pflA"
                     /locus_tag="PB780_001263"
     CDS             19810..20571
                     /gene="pflA"
                     /locus_tag="PB780_001263"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HDE0733327.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGNKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            20656..21582
                     /locus_tag="PB780_001264"
     CDS             20656..21582
                     /locus_tag="PB780_001264"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HDE0733328.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            21764..22660
                     /locus_tag="PB780_001265"
     CDS             21764..22660
                     /locus_tag="PB780_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HDE0733329.1"
                     /translation="MNYYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKK
                     EFSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLS
                     VDEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRATAGNEKYRFITLSSIEHF
                     PLTLANYFFVQKNQLPVTKAFKELAELIGDVNEAYFFDQIEVIVDRIRKNKYKNRRPS
                     IFHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEETAFLKRQIFSELS
                     KKFIPHAFSYIKEYRTVLVSKT"
     gene            22794..22919
                     /locus_tag="PB780_001266"
     CDS             22794..22919
                     /locus_tag="PB780_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDE0733330.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYSILWEELAA"
     gene            complement(23053..23685)
                     /locus_tag="PB780_001267"
     CDS             complement(23053..23685)
                     /locus_tag="PB780_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733331.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(23833..24375)
                     /locus_tag="PB780_001268"
     CDS             complement(23833..24375)
                     /locus_tag="PB780_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HDE0733332.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            24509..24898
                     /locus_tag="PB780_001269"
     CDS             24509..24898
                     /locus_tag="PB780_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733333.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            24902..25666
                     /locus_tag="PB780_001270"
     CDS             24902..25666
                     /locus_tag="PB780_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDE0733334.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRGSEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            25795..26742
                     /locus_tag="PB780_001271"
     CDS             25795..26742
                     /locus_tag="PB780_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDE0733335.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            26872..27612
                     /locus_tag="PB780_001272"
     CDS             26872..27612
                     /locus_tag="PB780_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733336.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKGNLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKMLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         9715 a         4759 c         5948 g         7387 t
ORIGIN      
        1 actaatagat ggaatttctt tataataaaa aagtgaattt atattgaaaa agaaaaaaaa
       61 caagtaaagc cggggaatat tccggtatga aaggaatgga gagaacaatg accatccaat
      121 ggtttccggg acatatggca aaagcccgac gagaagtttc tgaaaaaatg aaatatgtgg
      181 acatcgtatt tgaattggta gacgcacgat tacctttatc ttctcgtaat ccaatgctag
      241 atcagatcat tcagcaaaaa cctcgccttg ttttattgaa taaagcggat ctggcagatc
      301 cacaacagac aaagctgtgg caagaatatt ttcgtaaaca aggccatctt gcattagcta
      361 tcacggcaca agaaagtaaa ggaattaaag gtctagtacc tgcggctaaa gaagcattga
      421 aagaaaaacg ggcaagagat gctgctcggg ggatcaagcc tcgcgctatt cgggcaatgg
      481 tcttaggtat tccaaatgtt gggaaatcta cgttgatgaa tcgactagta ggcaagaaaa
      541 ttgcacagac aggaaataag cctggcgtaa caaaaggtca gcagtggctt cgtttaggta
      601 atgagttgga attgttagac acaccaggga ttttatggcc caaatttgaa gatgaagaaa
      661 tcggcaaaaa actagcgctg acaggcgcga tcaaagacca gcttgtccat ttagatgatt
      721 tagcgattta cggattagac ttttttgccc gatattattc aggaagaatc agcgatcgtt
      781 atggtttatc aatcgagcaa gaagaaaaat tggcaccgga attactgatg gatattacag
      841 caaaacgtgg gtataaagaa gactatgatc gcggcagcga attagtcgtg catgatattc
      901 gccaaggaaa acttggacga tacacactgg atcgttgcga agaaatggag caaaaaagtg
      961 atgactgaat ccatccaaag tatcaaagaa cgattgaaaa gcatacagag aatagatgat
     1021 cctacactag aatcattaag aaatgatcca cgcaaaggcg tgcagcaagc actggcttct
     1081 tttgaacgac gattgaaaaa ggaagcatta attaaggaaa aatatgaaga aatgttttgc
     1141 tttgaaaagc aagggtacgc acaaggtcac caatggattg ccggcattga tgaagtagga
     1201 agaggaccat tagctggacc tgtcgtggca gctgcagtta tcttgccgaa aaatgagatc
     1261 atattagggc tgaatgactc caaacaatta tcagagaaaa agcgagaact tctttatcaa
     1321 caagttcaag aaaaagccct ggcaatcggg atcgggatcg ttgatcagca gacaatcgat
     1381 gatgtgaata tttatgaagc aagtaaatta gcgatgatcc aagcagtaga aaaattgtcc
     1441 attgtaccaa gctatttgtt gatcgatgcg atgaccttgg atttgcctat ccagcaggaa
     1501 aaaataatca aaggggatgc acgatctgtg tcgattgctg ctgcaagtat cattgctaaa
     1561 gtatatcgag accaattgat gaaagaatat gacgtgcttt atcctggata tggtttctcg
     1621 cataatgcag gttatggaac aaaggaacac ttagaaggcc ttgaacgaca aggaattacg
     1681 ccaatccatc gtaaaagttt tgcacctgta aaaaaatatt tacaataaaa aacagccgct
     1741 tttacgtatc ttattagaaa gagagacgaa aggcggtttt atgatgtatc aaatagaaga
     1801 gaatttattg aaacttgctt atgcaaaagg aatcagtagt caaggaaaat gggcacttgc
     1861 taactggatg atgcagtatc cttataaaga aatcggattt gaagacatca ttaagatagg
     1921 taaaataacc acacatcggg agctgtttcg caaaagttgg caggaaattc acgctaactg
     1981 gggcaaaatc caatcgaaac agtcgtttat cacttgcttt gatcccaaat atccccctca
     2041 gctacttcac ttgacctatc cgccaattgt actgttttat aatggtgatc tatctttact
     2101 ttcttgtaac atgttaagcg ttgttggtgg aagacacact tctgctttag ccaaaaaaac
     2161 agtttattat ttactaaaac cagtggtaga agctggatat gtgatcgtca gtggctgcgc
     2221 gaaagggata gatacatacg gtcaacaagc agcaatctct catggtggac ggacaatcgc
     2281 agtcattgga acagggatag agcaagtcta tcctgctgaa aatcgtgact tgcaagaggt
     2341 gattgcaaat gatcatcttc ttttgagtga atatcctcct caatccggcc cgaaaaagca
     2401 tcgttttcca atgagaaatc ggattatcgc agctttgtct aaaggaacat gtgtctttga
     2461 agccaagaaa aaaagcggct ctctgataac agcacagcaa gcattggatc ttggaagaac
     2521 agttttttct gttccaggaa atattttaac agaacattct accggatgtc atcatttgat
     2581 ccaagacggt gcagtttgca cggtttcggg tgaagatatc ttggcagaat ttaaagaata
     2641 acaccatact tttcatttgc ttttcttgac aaacgttttc tgaatggata tgattagcta
     2701 cgatttgtat gaatcctctt ttaatagtag gaacacgaca atggaaagga gtccattcag
     2761 taatggcgta taaatattta gtgattgtag aatcacctgc aaaagccaag acaattgaga
     2821 aatatcttgg acgaaactat aaagtaatgg ccagtgttgg ccatataaga gatttaccga
     2881 aaagtaaaat gggtatcgat tttgaaaaca attatgaacc ccattatatt tctatacgcg
     2941 gaaaaggcga tgtcatcaaa agccttaaag ccgcagcaaa aaaagctcaa aaagtttact
     3001 tggcaagtga cccggataga gaaggagaag cgattgcttg gcatttagcg tacctacttg
     3061 ggttggatct gaaagaaaaa aatcgggtgg tcttcaatga aatcacaaaa gacgcagtca
     3121 aagcagcttt taaggaacca agaacgatcg atgtagattt agtagatgca cagcaagctc
     3181 gtcgtacctt agacagaatc gttggttatt cgatcagtcc tattctctgg cgtaaggtca
     3241 agaaagggtt aagtgcagga cgtgtccaat ctgtcgcttt aaaaattatt attgaccgtg
     3301 aaaaagagat ccgagaattt gttccagaag aatattggag catcgacggt aattttaaaa
     3361 aagctcgcaa gaaattcaaa gcaaatttct ggggaatcga cggtaagaaa aagaaattac
     3421 cagatgcaca aagtgtaaaa gaagtcactg ctagaattga tggaacagac ttcgaagtta
     3481 aaaaagtaga aaagaaagag cgcaaaagaa accctgcgaa tcctttcact acaagtagcc
     3541 tgcaacaaga agctgcccgt aagttaaact ttcgaacaag aaaaaccatg atggtagcac
     3601 agcaattata tgaaggaatc actttgggta aacaaggaac agtcggcttg attacataca
     3661 tgcgtactga ttctacccgg attgccgata cagcaaaagc agaggccgca gcatttattg
     3721 aagatacgta tggaaaagaa ttttctagac atgatcaccg aaagatcaaa aatgctcaag
     3781 gcgcacaaga tgcccatgaa gctgtgcgtc caacgagtgt gcttcgtaca ccagatgaac
     3841 tcaaacaata tttagataaa gatcaattga aactttatac attgatctgg tcacgttttg
     3901 tggctagtca gatgacacca gctattttag atacaatgaa agtgacatta gaacaaaatg
     3961 gtgtcatctt cattgcgaac ggttcaaaaa tcaaattcaa aggattcatg caagtgtatg
     4021 tggaaggtag agatgacggc aaagaagaga aagaaaatat tcttccagaa cttgaagaag
     4081 gcgatatagt ccaatctgtt gatattgaac caaaacaaca ttttactcaa ccaccagcac
     4141 gttttagtga ggctacgttg attcgtgcac tagaagaaaa cggggtaggg cgaccttcca
     4201 cttatgcacc aacacttgat acgatccaaa gacgctacta tgtaaaattg acacaaaaaa
     4261 gatttgagcc gactgaacta ggagaaattg tcaattcact gatctgtgaa ttcttccctc
     4321 aaatcgtcga tatccatttc acagcagaaa tggaaggaga tttggataaa attgaagaag
     4381 gcacggaagc gtgggtcaaa gtggttgatc gtttctataa accgtttgaa aaagaactga
     4441 cgaatgcgga agaaaaaatc gaaaaaattc aaatcaaaga tgaaccagca ggattcgatt
     4501 gtgatgtctg tggacatccg atggtaataa aattagggaa atatgggaag ttttatgctt
     4561 gcagtaattt tcctgattgt cggaatacga aaccaatcgt aaaagaaatc ggtgtgactt
     4621 gcccagtatg tcatgaagga caagtcattg aacgaaaatc taagaaaaac agactattct
     4681 atggttgttc ccgtttccca gaatgtgaat tcacttcttg ggataaacca gtgggcagaa
     4741 attgtccaaa atgtgacgga tatctggtcg agaagaaagt caaaggcggt aaacaagtag
     4801 tctgtgtaaa tggagattac gaagaagaca tccaaaaata atagaaaaga ggttctttca
     4861 ttgaaaatgg taaatgttgt tggagcaggg cttgccggaa gtgaagctgc ttggcaaatc
     4921 gcacaagcag gagtccctgt ccgtttatat gaaatgcgtc cagtcaaatc gactgaggcg
     4981 catcacacaa gtaattttgc tgaactagta tgttccaatt cattaagagg aaacagtttg
     5041 gctaatgcag tagggttatt gaaagaagaa atgcgccgat tggattcggt agtaatcact
     5101 tcggcagatg aaacagcagt gccagctggt ggtgccttag ctgttgaccg cgattctttt
     5161 tctgaaacaa ttacccgaaa agtcaaagag catccattag taacagtgat aaacgaagaa
     5221 attactgaaa taccggaagg aatcaccgta gtagcaacag gacctttgac ttctcatcca
     5281 ttagcagaaa gcatcaaagc attcaatggt tcagatggtt tttatttcta tgatgcagct
     5341 gcaccaatcg tagacaaagc tacgatcgat atggataaag tatatttgaa gtcccgctat
     5401 gacaaaggag aagcagctta cttgaactgt ccgatgacag aagaggaatt caaagctttc
     5461 catgaggcac taatcaatgc cgaagtggca gaacttaaat cttttgagaa agaaaaatat
     5521 tttgaaggat gtatgccaat tgaagtaatg gcacagcgag gattcaaaac gatgcttttc
     5581 ggacctatga aaccagttgg attggaagat cctaaaactg gcaaacgtcc ttatgctgtt
     5641 atccagctgc gtcaagacaa tgctgcagct tctttataca acatcgtggg ctttcagact
     5701 cacttgaaat ggggcgagca aaaacgtgtt ttccggatga ttcctgggtt agaaaatgct
     5761 gaattcgttc gctatggtgt gatgcaccga aacagcttta tgaattcgcc agaattgctg
     5821 aaaccgactt accaatcaac gaaacgcgaa gatctgttct ttgcaggtca gatgactggt
     5881 gtagaaggct atgttgaaag tgcagcgagc ggactacttg ctggaatcaa cgccgcacgc
     5941 ttagcaaaag aaatggaacc agtcgagttt ccacaagaaa cagctattgg aagtatggca
     6001 tactacatta ctcatgcgga aggaaaacat ttccaaccga tgaatgccaa ctatggtctg
     6061 tttcctgaac tgccagagcg cattcgagac aaaaaagcaa gatatgaagc aattgcacaa
     6121 cgagctttaa aagctaatga ggcaataaaa acagaattag aaaaaactgc aatcccaaat
     6181 gagtaaggaa actccatgaa gagggtgaga cggatagtct cgctcttttt cttgcgtttt
     6241 tcttaagaat ttctcaaaca aaatgtgagg aattgcgcca attgtgaaca aacttagaaa
     6301 tattggttaa attctgacaa tttatttgtc tttttataaa cgctgtgcta aattattctc
     6361 tgggacaaaa ggaaggtgag aaaatgacag aagccacgtg ggaacaaaaa tttttgcgtt
     6421 atttgattgt cgaaagagga tattcagaaa aaacgaaaga agcatatgaa gaagacattc
     6481 tccatttcaa acattttttg gaggaatctg gagacgctga tctgctgaag gcggaatatt
     6541 ttacggttcg tgtctatttg agtgcattag ctgatcatcg tcctagctat agcagaaatt
     6601 ctatcagtcg caagatagcc agcctacgtt cgttttatca gtatcttctc aaagaagaag
     6661 tgataaaaga aaatccattt tcttacgttc atctcaaaaa gaaaaatttg agactgccac
     6721 gtttttttta tgaaaatgaa atgcaagcac tttttgacag cgtagcagga gatagtcctt
     6781 tggatttgag gaaccgcgct ttactggaag tcctttacgg gtcaggcatc cgtttaagtg
     6841 agtgtagcag tttgtcggta tcagacattg attttgattc agaggtcatg ttgatccatg
     6901 gtaaagggaa caaagagaga tatgctcctc taggttcttt tgctcaagat gctctgcaag
     6961 aatatttcag ggaaggccgc caggtattga tgacaaaata tcacgaagag catgatatcg
     7021 tttttgtgaa tcatcatgga aaaccaatca cgcctactgg aattgaatat gtcttgaatc
     7081 aaatgatcaa aaaaagcagt ttggatagtg atatccatcc tcacatgctg cgccatacat
     7141 ttgctactca tttattaaat aatggggcag atttgcggac agtccaagaa ttgctaggac
     7201 atgccaattt atcgactacc cagatctatg cccatgtaac aaaagaaagt ttacaaaaaa
     7261 actatcgttc tttccatcca agagcgtaaa aataaggaaa agaacaaatt taggaggaaa
     7321 caaacatggt cgaatcacca tttcattcaa ccactatctg tgccgtcgaa aaagacggga
     7381 aatttgctat ggctggagat ggccaagtaa caatgggtga atctgttgtc atgaaaggca
     7441 ctgcaaagaa agtacgacgt atctacaatg gagaagttgt tgtaggtttc gccggaagtg
     7501 ttgcagatgc gtttacttta gaagaaaaat ttgaaggcaa actaaacgaa tataacggga
     7561 atctacaacg cgcagctgtc gaattagctc aagaatggcg gacacagcaa tctatgcaaa
     7621 aacttgaagc gatgctgatc gtaatgaatg ataaagaaat gcttcttgtt tcaggtacag
     7681 gagaagttat cacaccagat gatggtatct tagctatcgg atcaggaggg aattttgcgt
     7741 tggctgcagc ccgagcaatg aaaaactatg ctcaaaacga catgagtgcg aaagaaattg
     7801 cagaaaatgc gttaaatatt gccgcagata tctgtatctt tactaatcat aatattatcg
     7861 tagaagaact gtaagaggtg agtagatatg tatgaattaa aacaaacacc aagacaaatc
     7921 gtaagtgaat tagatgaata tatcgtagga cagcacgcag caaaaaaatc tgttgccgtt
     7981 gctttgcgta accgctatcg ccgtttgcag ttagatgaaa aaatgcaaca agatattaca
     8041 ccaaaaaata tgctgatgat cggacctaca ggggtcggta aaacagaaat cgcacgacgt
     8101 ttggcaaaaa tcgtcaatgc accatttgtc aaagtcgaag caacaaaatt tactgaagtc
     8161 ggttatgtcg gaagagatgt agaatcaatg gttcgggatt tagtagaaaa tgcgatccaa
     8221 atcgtagaaa aagagcagta cagccgtgta tacacaagtg ctaaaaaaaa tgcagaaaaa
     8281 cgtttggtaa aaatcttggt acctggtatc aaaaaagaac agaaacaatc cactaatcaa
     8341 tttgaacaaa tgatgaatct ttttaatgct agtcagcaaa ataccgctga accgcaagaa
     8401 gaagtgacag aagatattcg aatcaatcgc cgtacagtct tggaacaact acaaaagggt
     8461 ttactggata gtcgtgaagt aaccattgaa gttgaagacc aaaagaaacc tgcaccaatg
     8521 atgaacaatg gattggagca aatggggatc gacctaaatg aaactctagg tgctttgaca
     8581 ccgaagaaaa aagtggaacg gacggtaaca gtcaaagaag ctttagagtt attgatcaag
     8641 gaagaatcag caaaattagt caaggatgct gatatccata gtgaggcaat caaattagcg
     8701 gaagaaagtg gaattatttt tatcgatgaa atcgataaga tcacttctaa atcacagcaa
     8761 tctggagaag tatccagaga aggcgtccaa agagacatat tgccaatcgt cgaaggttca
     8821 caagtaaata caaaatacgg gatcatccaa acagatcatg tcttattcat tgcttcagga
     8881 gcgttccatt tatctaaacc gagtgatttg attccagaac tccaagggcg tttcccaata
     8941 cgggtagaat tagatgattt gacagctgaa gattttgtga aaattctgac tgaaccgaat
     9001 aatgctttaa taaaacaata tgtagcattg attggtacag aaaatgtgtc agttacgttt
     9061 acaaaagaag caattgaacg gatcgctcaa atcgctttcg atgtcaaccg tgacacagat
     9121 aatatcggcg cacgcagact gcataccatt ttggaaaaac tactagaaga tctattattt
     9181 gaagcaccag atatgcaaat gggagaaata acgatcacag aagcatatgt ggatgacaaa
     9241 cttggctcaa ttgcggcaaa tgaagattta agtcgctata ttttataaaa aatcggagga
     9301 cgacaatgac agacttgcta acgaaaaccc gcaagataaa cacacttttg cagcaaaaga
     9361 atacttttga catgcaagca gatttgcctt acgataaaat ggctgtcaca ctaggtggta
     9421 ttcttgacag caacacctat atcataggaa gtgacggagt attactaggt tataatgaac
     9481 gtcacgatgt gaacaatgaa cgaatcaaaa caatgtttgt tgagaaacaa tttcctgaat
     9541 cttatactga tacagtggca cagttgatga aaactgaagc taatatccca attactagtg
     9601 atattactgc atttccggta gagtcaagag agaaatatcc atttggtctg acgactgtcg
     9661 ttccgatctt tggagctggc gaacgtttgg gtacgattat cttatctcgt atggaacaat
     9721 cgtttgcaga tgacgatcta gtcttagcag aatatggagc tacggttgtc ggtatgcaga
     9781 tcctttatca gaagtctcga agcatcgaag cagatgtacg gagtgcaacg gctgtccaaa
     9841 tggcaatcaa cacattatct tatagcgaat tgaaggctgt acaagctatc tttgaagcat
     9901 tggatggaga tgaaggtcgt ctgactgctt ctaatattgc cgatagtatc ggcatcactc
     9961 gttctgtgat cgtcaatgct ttacgaaaat tggaatcagc tggaatcatt gaatctcgtt
    10021 cgttaggcat gaagggaact tatttaaaag tattgaattc ccgttttaaa gacgagttag
    10081 caaaacacag ttattagaag taaggggact ggggcagata gaaatgtttg ttccagcctt
    10141 cccattttaa aaagtaagtc gaagttggag gaatggcaat gactgttatt attcaaaatg
    10201 accagctgat cgccgaaatc gctgaacacg gtgcggaatt gatcagtctg aaatcaaaag
    10261 aacatggttt agagtatatc tggcaaggtg atccagcgta ttggggcaga catgcaccgg
    10321 ttttattccc ttttgttgga cgtttaaaag atgatcagta tgtttatcaa gggaaaacat
    10381 atcaaatggg gcaacacgga tttgctcggg atatggattt tgaagtcatt gaacatggac
    10441 aagaaatggc tagtttcatc ttaaggagta cgccggagac attagaaaag tatccatttg
    10501 attttgaatt agtgatcagc tatgaattag gaggagacgg catcacggtt cattatcaag
    10561 tggcaaatac cggtgaaaaa gaaatgttct tttctatcgg tggacatcct gctttcaatg
    10621 tacctcttga agaaggattg tcatttgaag atttctatct ggcattttct ccattgaaat
    10681 caagattacg cctaccattg aaaggcccat tcatcgatat ggatcaaaag acgttaggtc
    10741 agacaaatac gaatctggct ttgaatcgtg aaatttttga gcaagatgcc ttggtttttg
    10801 aaacaagggg tctaaatgcg tatgccattc gaagtgaaaa atcttcccat agcgtaactt
    10861 taagctataa agatatgcct tatgtaggga tctggtctcc ttatccaaaa gaagcgcctt
    10921 tcgtttgtat cgaaccgtgg tgcggaatcg ctgacgcggt gaacagtagt ggaaacttga
    10981 tggaaaaagt agggatcaat caactaggtg cccatgaaat cttcaagaca aaatattcca
    11041 tcacagtgaa gtaagaggtt gtgaccgaga agttagcagg gaacaccgtt taccaaggtg
    11101 tgtaaaaaag ctatcctgta agtaataagg gagtgtgaca tcgatttgtc acactcccgt
    11161 tttatctctt tggaatgaaa aagtatcagg attttttctt gctgctattc aaaccaaacg
    11221 ggattcgact ttcagtatgg tttctgatac gcttgatatt gtctttatgc cgataaaaga
    11281 taaaggaaga aactaaaaag gcaatgatcg tcagtaacca attgggctta gccaaaatca
    11341 caggagctac ataaggaagg acgatcgtag ataaagtgat caatactgca ctgatcatac
    11401 tgctcaaact caccatactt gtacaaaata aactaacgat aaagatgata caagaataaa
    11461 caaaaaatac aggattaaat cctaaaagca ttcctgcact tgtcgcaaca gcttttcctc
    11521 ctttgaaccc agcaaaaatc ggaaatgtat ggccaagaat ggcgcatacc ccaaaccaaa
    11581 gagggttaat cccagaaagg tggaagatca tcggcaaaca agtagccaat gtacctttta
    11641 aaatatccat aagcaggaca acaatcccgg ctgttttgcc taatacacga aatgtattgg
    11701 tcgttccagt attaccgctc ccgtgttgtc ggatatcttt tttgaaaaac aatttgccaa
    11761 tccagacacc ggaaggaata gaacctaaaa gatatgctaa aataagtaat aagatcaatt
    11821 tcatttttta ccctctttca agttgaaaac tatcctttat tttagcatga ataggtaaca
    11881 ggaacaattg aaaaaatgaa agatacaagt ataatagatg gaataaaaag aaaagcaagg
    11941 ggtggcagaa atgtctgtaa aaaatccaac acaagaaacg attttcaact atttggaaga
    12001 agcaaaaaat attgccgtcg taggcttgag caataaacca gaacgaacaa gttacatggt
    12061 tgcacaggta ttgcaagaca atggatataa aatcatacca gtaaatccat tattggctgg
    12121 tgaagaaatt ctaggcgaga aagtctatgc gaatttgaca gaaatcgatg atccaatcga
    12181 tatcgtagat gtatttagac ggagtgaatt tttacctgaa gtagcagatg attttttgaa
    12241 aactgatgct aaagtgttct gggcacaatt agggattgaa aacaaagaag cagctgagat
    12301 gctaagaaaa gcaggacgta cagatatcgt gatggatcgt tgtattaaga ttgaacttgg
    12361 taagttgaaa gaaaaccaaa aataaaaatg accacaaaga agagatgatc gatagagggg
    12421 aatgtcgaaa aagcggttag gagataatta aaattgattt ctcgtctacc ctcttttttt
    12481 tcacgacctt ttctagtatg ataatcgtgg tgcggttttg ccgcaaagta ttattgaagg
    12541 agcgtttgcc ttggcaaaaa aagtaaacaa cgaatacaat gactcgtcca tccaagtatt
    12601 ggaaggatta gaggcagtta gaaaaagacc agggatgtac attggatcca ctgacagccg
    12661 aggcttgcat catttagtct atgaaatcgt cgataatgca gttgacgaag cattatcagg
    12721 ttatggcagt gagattgatg tgaccatcca tgaagacaat agtataactg ttgcagatag
    12781 cggacgtggg atgcctgtag gaatgcatgc ttccggaata ccaacagttg aagtgatttt
    12841 tactgtgctg catgctggcg gaaagtttgg tcaaggaggg tacaaaactt ctggtggact
    12901 gcacggtgta ggagccagtg tggtaaatgc cttatcgaaa tggctaactg taacgatcgt
    12961 tagagatggc gtagagtatc agcagaaatt taaaaacggc ggtaaacctg acggtacgtt
    13021 gaaaaagata ggaaaaacaa aaaaagcaaa cgggacgact gtccattttt taccagacga
    13081 tacaatattc agtacaacga aattttcata tgaaatttta gccgaacggt taagagaatc
    13141 tgcctttctt ctaaaaggcg ttaaaatctc tctttctgat cttcgtggag aagtaccagt
    13201 caaggaaatt tttcattacg aagaaggaat caaagaattt gttgattatc tgaatgaaga
    13261 aaaagataca ttgacaccag ttgtttattt ttcgggagaa aaagaaggta tcgaagtaga
    13321 ggtcgcttat caatacaatg acgggtattc agaaaatgtc ttatcttttg tcaataacgt
    13381 tcggacaaaa gatgggggaa cccatgaagc tggaatgaaa gcggcaatga cgaaatctta
    13441 taatgaatat gcgagaaaag ttggtttgct gaaagaacgg gacaagaatc tggaaggtag
    13501 tgatttcaga gaagggttag ctgctgtact ttctatccgt gtacctgaaa atcttctgca
    13561 atttgaaggg caaacaaaag aaaaacttgg tacgccagtt gctcgtaccg tagtagataa
    13621 cgtcatcagt gaacaaatgg gcttttattt gcaggaaaat agcgaaatga gccagatgtt
    13681 ggtccgtaaa gcaatcaaag ccagagaagc gagagaagca gcacgtaaag ccagagaaga
    13741 aagccgaaat gggaagaagc gtaaaaaagg cgaatctctt ctttcaggaa aattgacacc
    13801 cgcacaatcc cgaaacccta agaaaaatga attgtattta gtcgaagggg attctgctgg
    13861 cggatctgct aaacaaggaa gagacagaaa gttccaagca attctacctt tacgaggaaa
    13921 agtcatcaat accgaaaaag ctaaaatgca agatatcttg aaaaatgaag aaatcaatac
    13981 gatgatctat acgattggtg caggagtcgg tcctgagttt tctatcgaag actgtaatta
    14041 cgacaaagta atcatcatga ccgatgcgga tacagacggc gcccatattc aagtattact
    14101 gcttactttc ttttatcgtt atatgaaacc gctgattgaa gctgggaaag tatatatcgc
    14161 gttaccgcct ttatataaag tatccaaagg acaaggaaag aagcaagtca ttgaatatgc
    14221 gtggacagat gacgaattgg cagcaatgat taaaaaagtc ggcaaaggat atatgctgca
    14281 gcgatataaa ggacttggag agatgaatgc ggaacaattg tgggaaacaa cgatggatcc
    14341 gacttcccgt acgttgatcc gcgtccgtat cgatgatgct gcgcaagctg aacgtcgagt
    14401 aaccacactg atgggtgaca aagtagaacc tcgaagaaaa tggatcgaaa atcacgtgca
    14461 atttacgctc gaagaagatg gcagtatctt agataaaaaa gaggatactg aaatctctcc
    14521 ttcagtttct aatgatttat tagatgaaga acgagcagat aaaaatgaaa acaatcagtt
    14581 atttgaagta gaataggagg tcacctgatg gaaaatcgac aagaagtaca agaattaact
    14641 ttagaagaag tgatgggtga ccgcttcgga cgttattcca aatatattat ccaagaacgt
    14701 gcactgcctg atatccgtga tggattgaag ccagtgcaac gccggatatt atttgccatg
    14761 aacaaagatg gcaatacgta tgacaaagga ttccgtaaat cagcaaaatc tgtcggaaat
    14821 atcatgggga attatcatcc tcatggtgac atcagtattt atgaagcgat ggtccgcatg
    14881 agccaagact ggaagttgcg ggaagtactg atcgaaatgc acgggaataa cgggagtatg
    14941 gacggtgatc cacctgctgc gatgcgttat actgaagcca gattatccaa gttaagtgga
    15001 gagatgctag cagatatcga aaaagaaaca gtcgatctcg tgtggaattt tgatgataca
    15061 gaaaaagaac caacagtcct tccagctcga tacccaaacc ttctcgtcaa tggatcgaca
    15121 ggtatatctg ctggatatgc aacagaaatc ccgactcata acttagctga agtgattgat
    15181 ggcacaatct atatgatcga tcatccacaa gcgagccttg aaaaattgat ggaatatatt
    15241 cctggacctg actttccaac aggagggatc ttgcaaggaa aagatgaaat caaaaaagct
    15301 tatgaaacag ggcgaggaaa agttattctt cgttcgaaaa caaagataga ggcaatgaag
    15361 ggtaataaac agcaaatcgt gatcacagaa atcccatatg aagtaaataa agcgaccttg
    15421 gtgaaaaaaa tggatgagat ccgtttgaat aagaaaatcg atggaatagc tgaagtacga
    15481 gatgaaagtg atagaacagg tctgcaaatt gtcgtcgaat tgaaaaaaga cgccaatgct
    15541 caaggaattt tgaattattt gttcaaaaac actgagctac agatcaatta caatttcaat
    15601 atggtcgcta tcgaccatat gactccgcat caagttggac tgaaagacat tttaagaagt
    15661 tatatcgagc atcgaaagca agtcataaca aaacgtagtc aattcgattt agccaaagct
    15721 caaaaaagac agcatatcgt agaaggattg atgaaagctt tatcgattct ggatgaagta
    15781 atcgtaacga ttcgtgaaag taaagacaaa aaagacgcca aaaagaattt agtcgacgtc
    15841 tttcaattta cagaagaaca agcagaagca attgtcacat tgcaattata ccgtttgacg
    15901 aacacggata tcacagagct tcaaaaggaa tccgaaagct tgattgcgca aatcacagag
    15961 ttgaataaaa tcctttcaaa tgacaaagag cttttctctg ttatgaaaaa agaattgcga
    16021 gaagtgaaaa aaaattattc ttccgctcgt ctaacgacaa tcgaagacga aatcgaagaa
    16081 atcaaaattg atacgcaagt cttagtggca caagaagacg taattgtcag tgtgacgcga
    16141 gaaggctatg tgaagcgaac aagccttcgt tcttatagtg cttccaaacc agaagaaatc
    16201 gggatgcgag aaggcgacta cttgctatac tcaggtgaat tgagtacatt ggaccatgtt
    16261 cttctgatca caaataaagc aaatgtgatc taccgccctg tccatgaact acccgatttg
    16321 aaatggaaag atgctggtga gcacatttct caaacaatct ccaatctgtc tgtagacgaa
    16381 tcgattttag ctgttttccc atatcaaaaa attcaagcag agaaaacatt tgtattcatc
    16441 agcaagaatg gattgatcaa gcaaacacga atgacagagt tcgaaccatg gcgcacctat
    16501 aagagccgtc cgctcagctg tatgaagcta aaggcggaag aagatgaact gattgctgtg
    16561 tatctgaaag aagatcagcc aggcttagat gttttcttag taacgcatca aggaatgggt
    16621 ttgcgctatc ctttaacaga tgttcccgtt gttggagcaa aggcagctgg agtgaagtct
    16681 atgaatttga aagatggaga taggattatc aacgggctgc ttgtgttagc agaaggtgat
    16741 acgccgatcg taatcgttac tcagcgagga gctgttaaac gcatgcttgc tcaagaaatc
    16801 agccaaactt cacgtgcgaa gcggggagtg acggtcatgc gtgagctgaa aaaaaatccg
    16861 caccgtatta tctatatgtc ggaaggaaga tcaaaagaaa taaccttgat gaatcaaaaa
    16921 gggcagcagt taacgattga cccaactgat ttccctatag gtgatcgaac atcgaatgga
    16981 tcatttgcga tggatgagaa aaaaggtgga gaagtcatcg aagttatcga tgctcctgag
    17041 attttcatcg agtcgtaact gttgtagtat tttaatgaag agacgccatc tgtattaata
    17101 caggcggcgt ctctttttca ttcattttta ataaatcctt atacgtttgc aaaaaaagat
    17161 gaaagtgttc acaatgctta aatgttggta tgtcaacgtt gatttaaata tatgtaaata
    17221 aaacataaaa attgttaaat aaaaaacaag aaaacccttg caaagatagg gtcctgtgtt
    17281 atactaatca cgtaaaaact gttacataaa acagaaattt aggaggtcca tacaatggag
    17341 caatggaatg gatttaaagg taccaaatgg aaagaaggcg tagacactag agactttatt
    17401 caaaataact acactgaata tcgcggtgat gacagctttt tggaaggccc aacagaagca
    17461 acagaaaaat tatgggatga attgcaaaag ttaaatgata tccaattcga gcgtaacggt
    17521 gtctatgata tggacacaga aatcgtatca actattactt cacacgatgc aggctatttg
    17581 gataaagatt tggaaaaaat cgtcggtcta cagacagaca aacctttgaa acaagcattc
    17641 atgccgttcg gcggaatcaa tatggctaac aatgcgctaa aaagtaatgg ctatacacca
    17701 aacgatgaat tgacacatat tttcactgac tggcgcaaaa cacataatca aggtgtattt
    17761 gacgcttata ctgctgaaat gcgtgctgct cgtaaaaaca aaatcattac agggcttcca
    17821 gacgcttacg gtcgcggacg tatcatcggt gactatcgtc gtgtggcatt gtatggaatc
    17881 gactatttga tggaacaaaa gaaaaaagat catgatatga ctggttatca tacaatgagt
    17941 gaagaagtga ttcgtttacg tgaagaaatc actgaacaat atcgtgcatt agcgcaaatg
    18001 aaagaaatgg ctgcgaaata tggctatgat atttctaaac cagctgcaaa tgcaaaagaa
    18061 gctgtccaat ggctatactt cggatattta gcagcaatca aatcacaaaa tggtgctgcg
    18121 atgtcaatcg gacgtatctc agcattcctt gatatctata tccaacgtga tttggataat
    18181 ggagtaatca cagaaaaaga agcacaagaa ttgatcgacc acttagtaat gaaattacgt
    18241 atggtcaaat tcgcgcgtac acctgaatat aaccaattat tctcaggatt ccctatctgg
    18301 gctactttat ccattgcagg tatgggatta gatggtcgtt cattagttac aaagaatgat
    18361 ttccgtctgc ttcatacatt aacaaatatg ggaccttcac cagaaccaaa cttgactgtt
    18421 ttgtattctg aacaccttcc agaaggattc agaacatatg ctagcagaat cgctatcgaa
    18481 agttcttcga ttcaattcga aaatgataat cagcttcgtg aacaatgggg ttctgatgat
    18541 tgtgcgattg cttgctgtgt atcaggaaca gttgtcggaa aagacatgca attctttggt
    18601 gctcgtgcta acttggctaa aacactatta tatgccatca atggcggagt cgatgaagtg
    18661 actggcgccc aagttggacc agaatatcgt ccaatcacat cagaaatact agactttgaa
    18721 gaagtgaaac ataacttcat gaatatgatg gattggttag cagaactata tgtcaatacg
    18781 ttgaacatca ttcattatat gcatgataaa tattcttatg aagcagctca gctggctttg
    18841 atggattctg acttgcaacg cacatttgct acaggtattg ctgggatttc tcatgcggca
    18901 gacagcttgt cagcgatcaa atatgcaaaa gtaaaagcaa tccgtaatga aaaaggaata
    18961 gcagttgatt ttgaagtgga tggcgacttc cctaaatacg gaaatgatga tgaccgtgca
    19021 gatgaattag tttcatggat cttggaatac ttcatgggac aaatcaaacg tcataaaact
    19081 taccgcaact caaaaccaac aacttcatta ttgacgatta cttcaaatgt tgtttacggt
    19141 aaaaatacag gaaatactcc tgatggccgt gaagctggaa aaccacttgc gccaggagca
    19201 aatccaagtt acaatgcgga acaaagtggg ttattagcta gcctgaactc aacagctaaa
    19261 atgaattacg gctgtgcatt ggatggaatt tcaaataccc aaacaatcaa tccttcaggt
    19321 cttggaaaag aacaagatac aagaatcgaa aacttgcgta atgtcttaga tggatacttc
    19381 tcaaaaggag cttatcattt gaatgtcaat gtattcagta atgatttatt gcgtgatgca
    19441 atggaaaatc caatgaaata tccgaacttg acgatccggg tatctggtta tgcagttaaa
    19501 ttccgtgact tgacaagaga acaacaatta gatgtcttga tgagaacttc acacgacaga
    19561 atgtaaaaag ttcgtgaaaa aagtttttcg acttagcgtc gaaaggacgt tgaatagcca
    19621 tcgcatcaaa atcgaataat cagtatgctt atttgaaaga aacgatttac aggggcagga
    19681 cttgtatgtt ccgtccctgt aaattttaca cataacgaag cggcaattgc gaaaaatttt
    19741 gttcagaaag cttcgcttcg acagctaact tgtacttaaa atgaaatcaa agaaaagatg
    19801 tgaacttaaa tggaagaaaa aacaatcggc tatgttcatt ctatcgaaac tttcggttca
    19861 gtagacggac ctggactgcg ctttgtcgtg tttatgcaag gatgccgcat gcgttgccaa
    19921 ttttgtcaca atccagatac ttggaatatt ggcggaggga aagaatacac agcagatgaa
    19981 cttttagata aagcagaacg cttcagacct tactggggaa ataaaggtgg tattactgtt
    20041 agtggtggag aacctctatt gcagatcgat tttttgatcg aactatttaa aaaagcaaaa
    20101 gagcggaaga tgcatactac attggataca tgcggaaaac cttttacgta tgaagaacct
    20161 ttcttttcac gttttcaaga attgatgaag tatacagatt tactgttatt tgacatcaaa
    20221 cacatcgata atgaagagca taaaaaacta acgcattggg acaatgaaaa tattttagaa
    20281 atggctcaat atttgtcaaa aatcaacaaa ccggtttgga ttcgccatgt actagttcct
    20341 gagcgaagtg attatgatga atatttgatc cgactagaca actttattca aaccttgtca
    20401 aatgtagaca gagtagagat tcttccttat catacgatgg gaaaatacaa gtgggaaaca
    20461 cttggtttga aatatccatt agaagggatc gaaccaccta caaaagaacg tgtcgaaaat
    20521 gctaaacgat tgcttcatac agaagagtat acaaaatatt taacgcgata ggtcgcatcg
    20581 gaaagaagat aagtattgct tctttccgtt tttttgctat tattaaatag gagaaatcac
    20641 aaaggagcga atgacatgtc aaaaatttta gtttttggtc accaaaatcc agatacagat
    20701 gcaatcgggg cagccattgc gttttcacac ttgcaaaatg aattaggtaa agaaacagaa
    20761 gcagttgctc taggtacacc taatgaagaa acacaatacg cattagacca ctttcagcta
    20821 tctgcaccac gagtagtaga gtcagctaaa gcagaaaatg cagatgtcat gcttgtggat
    20881 cataatgaat tccaacaaag tatttcagat atcagcgaag taaatatttt agctgtagta
    20941 gaccaccatc gtattgcaaa ctttgaaaca gctaatccat tgtactatcg tgcagaacca
    21001 gttggatgta caagtacgat tattttaaaa ctatataaag aaaaccaagt aacaattcct
    21061 aaagaaatcg caggaatcat gctctcagct atcatttcag atacattgtt gttcaaatca
    21121 ccaacatgta cgcctgaaga tgaagcagct gcaaaagaac ttgcagcaat cgctgaagtc
    21181 gatctaaaca cttatggact tgaaatgttg aaagcaggaa caaatctaag tgataaatca
    21241 gctgcggttc ttcttgattt ggatgcaaaa agtttcccaa tgggcagttc caacgttcgt
    21301 atcgctcaag tcaatacagt ggatcttaat gaagtgatgg atcgacaagc agagctagaa
    21361 gcagcaatgg aagatgaaat gaaagcaaat ggctacgatc tgttcgtatt agttgtcaca
    21421 aacatcttag acagtgattc agaattactt gttgctggta aaggaaaaga aaaagtagaa
    21481 gaagctttcc aaacaacttt gaccaacaac cgtgctttgc ttaaaggtgt cgtttcacgt
    21541 aaaaaacaag tcgtacctca attgacggct gtatttagct aatcaaaggg tagtatgaag
    21601 cattcaaaat gaatggataa gcgattgata taaaaagaaa catagaacat actaaaggcc
    21661 gaagaaatgg aggagaataa cagtcaatta tgactcttgt tcttcctcct ttttacgtaa
    21721 aagcctttta agtaaaataa aacggataga aaagagaaca tatatgaact attattattc
    21781 aaaaaataag gaaaactttt atcaaaaact gaccgatgat ccgctttttt cttcattgac
    21841 cgattatcta tatgaacatc gagaacaaga aacgattcta agagaattga aaaaagaatt
    21901 ttcgcaaaat aaattttccc attttcttga tctattgatc gatgcaggat taatcaagag
    21961 ggaagaacga agatatcatt tgaactttcc aatatttgat cctaaagatt acttgcaaca
    22021 agccacttcg gctgctgaaa ccatagctga acaattgaag agactctctg ttgatgaaca
    22081 aaaattagcg atgggagaag ttatttgggc gtattgtttc gaagatgaac ggaaagaagc
    22141 gtatttttat ggagtaagaa acagccgaga gacagaattg ctgagggcaa ctgcaggaaa
    22201 tgagaagtac cgttttatca cactttcctc tatagaacac ttcccgctta ctttggctaa
    22261 ttactttttt gtacagaaga atcagcttcc tgtaacgaag gcattcaaag aattagccga
    22321 acttattgga gacgtaaatg aagcgtactt ttttgatcaa atagaagtta tcgttgacag
    22381 gatacgtaag aataaatata aaaatcgccg gcctagtatt ttccatcagt ctctacttgt
    22441 gacgaatact ataaaggaag aagaatcatt cacattagaa ttacctattg tcgaaaaaaa
    22501 caattttgag attgaattac caacattgga tccttcattg acaatggagg aaacggcctt
    22561 tcttaaacga caaatctttt ctgagctgag caagaaattt ataccgcatg cattttctta
    22621 tatcaaagaa tatagaacag ttttagtgag taaaacctaa gagagcagta agcaaaaatt
    22681 atactgactc ttgcaggagg agcctttctc ttcctttatg gttaaaagaa ttatttgagt
    22741 attttgccta tctaccacct attggattca ctacatggtc tggaaacaaa ggaatgcaaa
    22801 aaaatggtga gaaatgtgga atgacaaagg aagtagtgat aagaaaagtt agatttttaa
    22861 ataatcaata ttatgattca gtcaagtata gcattttatg ggaagaacta gctgcttaga
    22921 ttaggaagtc catttgatga tccagaatca ctaagaaacg aatggcgagt cattaataat
    22981 ttatccaatc ttgggttatt cttttagatt gaagtgttta aaactaaaaa agcatttatg
    23041 aagtcgtaat agtcaggctt caaaatgctt tttattttgt ttgttttttc tttgccacat
    23101 aaatacacct agactgacga tgaagagcat atccaacaga tttcctgcga cttggatcgt
    23161 cgaagggttg gtcagtggca ttaaagcaag gacaaaaaag gaaaacaagc tgtaaataaa
    23221 acaggtgaca ggaactaatc gaaagaagag tggacatttc aattggctaa aaatgagtga
    23281 aaaaataaaa taagtaaaaa tggataaaat cacgactaaa ccattcatga taaagaagaa
    23341 aagcgtattg tgattaaacg aataaaaaag aattgctaaa aaagaaaacg ttttaaatgc
    23401 aatcaatacg cttgaagctt cgtttaacgt accaggatgt cgatgaaaaa aataggcacc
    23461 gaaataaaca gaaaacagta agccgcaaag agaagacaaa atcaaaaaaa acggattttc
    23521 ttcggcttct gtgatatcag aatgatccaa aagcaaccag cctaatgcac tagaaggcgc
    23581 atgtgaggtc ttaaggtatg aataacatag aaaaaataag atcgtcaaag ttgcaaaaaa
    23641 agagcctaaa tgtaaaaaat tagtggttct acgttgaatt tccataatat ctccaaatta
    23701 aattgataag tatataaagt ataataacaa attgatgagt atatgtcgat gttttttaat
    23761 caaaataata aataaaaact gtctaacatt taaaatttaa tttagatatt agacagtttt
    23821 taagtttgtg agttattttc cttgtcgttt tctcactttt ttagtccaga aaccacctga
    23881 aatatatttt ggctcataag aaatgataaa agcacgctct tcgatttctt tgatcaggcg
    23941 atacagttca cgttcgcttt ttctaggaga aagtatttcg agaactagcc gctcaccttc
    24001 cagccccatt gcagaagtct gtgtcacacc ataaccgtgt tctctcagta tacccggcaa
    24061 acttttatct tctgcgctat ttgctggcaa aatcgttgtg accatgatgt atcctaatgc
    24121 caatttgtct tcaatcaaaa ttccgacact gatacctact gcgtaaccta acgcatagac
    24181 aaataaattc agtggattat ctaaacgatc taaaacaagg gataagccga cgatataaat
    24241 cgtgatctcg accatactga caagtggagc aatcagtcga tagcctttca ttgtcaacat
    24301 gaaacgtaac gtgttcagag tgatataagc aaaattaatg aggaagatca tccccaacat
    24361 ttttggatca atcatttgta aaactccttt catttatata acaacaatat caaaaaaaat
    24421 cgtaagtgaa gaaaaattga aactttatac atacaaaaaa tgataaaatg attataatag
    24481 aattataaag ataggttagg tgattaaaat gaaaacggtc aatcgtgtgt tttgtgccag
    24541 ccacgtgcta agtctccttc tcagtatata cttaggtgtc tattcgattc aacagccaat
    24601 tgaggtagag aacattgttt ttcagatgtc gctgacagat ggtttaattt taagcgtttt
    24661 tttctttttg atactgttta ttggtaatat tttcggtagc gttgccagtt ttttgaattt
    24721 ttctatttat ccactccttt cgctagcatt aggagtggtg ggactttgtt ctctgtgctt
    24781 atttcccgag ccattcagtc ctgcatttat attatttggt acactgaacc tgttccagac
    24841 aactgttgga gcatggttgc tatggcgatc aggaaattta atgaaaatag gtgagtaaca
    24901 attgaatcca attatattga aacgttctgt ccatcatatt ccagtgttag aagttgttct
    24961 gaaagaatta agagaggaac gtattcccgt tgtcatctat tatcacggat ggcagacaag
    25021 taaggagtta gttttgacgc aaggaagaaa aattgcgcag cagggcattc gtgtgatcct
    25081 gccagatgcg atgaatcatg gggagcgcaa acagccggtt tcttctattc catcctatac
    25141 attttgggat agtatctacg gaaatctgtt tgaatttgac acacttatca agcatttaga
    25201 aaaaagaggt cttctacatg atcagcttgc agttggcggg gtttcgatgg ggggaatgac
    25261 aacatgcgcc ttgttggcta aacatcctca tatagtagcg ggcgtgtgtt tgatgggttc
    25321 ccctgcacct ttggaatatg gaaaagaaat cgtaaggcgc ggatcggaat ataattatgt
    25381 cgtaccaaaa gactattttg atttgatttc atgggttaag agctatgatt tgtcattgca
    25441 gccggaaaag ttagctggac gtcctctgtt cttttggcat ggacaagctg acgaaaaaat
    25501 cccttatata caaacggaac gatttgtaga aaaaaatcgg cataagtctt acggaaaaga
    25561 aattgttttt cgttcctcaa aaacacagcg acatatggta caaataccac taatggaaga
    25621 agcttccact tttttagcaa aaaatttgat aaaaaagtat aaataattga aacggaaaat
    25681 cactcatgac gatagaggat tttccgtttt ttattttggg ctataataaa ctctgttagg
    25741 aaaatttggt ataattgata gtaagaatga caacatgtta gaaaggaaga gagcatgccc
    25801 actttagcag atgttgcaaa acgtgccaat gtttcaaaaa tgacggtctc tcgagtcatc
    25861 aatcatccgg aacaggtgac tgacgaattg aaagagttag ttttctcagc aatggctgaa
    25921 ctggattacc gtccaaatat tgctgcaaaa gcacttgtca cgaatcggtc gcagatcatc
    25981 aaattattta tactggaaga aatcgacaca actgagccct attacatgaa tttgctgatg
    26041 ggaatcgcta aacggatcgg taaagcgcat tattctctcc aattagttac aaatgatgcc
    26101 ttcgacgtcg gttcttgtga tggatacatc atcacaggtg tcagaaaaaa agacttcgat
    26161 tggatcgatc gtttggaaaa accagttgtc ttgtttgggg aaaattctga aggatatgat
    26221 tatgtagaca gcgataatga atatggtaca gcaaaagcaa cgcaatatgc cttagaaaga
    26281 gattatcaaa cgattattta tatcggtatc gaagtggacg agccttttga aatttcaagg
    26341 gaaactggtt atttatctgt gatgaaggaa gttgatcttc ctagtaaaat cttgcggttt
    26401 ccaaatcatt cgacagaagt agaaagatat atgacagaaa actgggcaga ttttacagat
    26461 aaaacttgtt ttgtatgtag ttcagatcgg ctagcactag gagcagtaag agggattata
    26521 aatgctggag gacacttgcc cgaagaattt ggtgtgattg gatttgatgg ggtattttta
    26581 gaccaaatca gctcacctaa attgacaacg atcaagcaag atattatacg tatgggtgaa
    26641 gtatgtggag aaatgttgtt aaaaaaaata gaagaaaaag gggcgagtca aggttatcgt
    26701 cgtttttttc ctgaactagt tatcagagag acaacgaatt aagtcttatt actatatata
    26761 tatttatgaa attcatcgct attttcttaa aataatcagc gcattgctac tgaaaacaat
    26821 gaatctgtgc tattattgag aaaagttcca tttttgagaa ggatggtttg aatgaaagaa
    26881 atcagacgaa aacatttccg ttttcctgcg tacgatgatg agttaggcgt aaaattaacc
    26941 aacgacaaga agcgagtatt atttgacgga caagaagatc ttttgactga acagaatcag
    27001 tccccgtttg agcagatgaa agaatttcat ttcacgaaca cgaacaccga gaaaatccgt
    27061 cgcaagcgtc gtgcaacaaa tggattgaag tcagctccgc gtaagggaaa tttggaaaag
    27121 cataaaggaa aattaccgga ttataccggc cattaccaat cagaaatcac atcgacagga
    27181 cgaaaaaaac tttttggtga ttcctctcgt tctgtcagac aaagtgaaac aaggacagag
    27241 tccaagcata ctacacgaaa cgcatctgtt gacacgaagg tgcgggagcg ttcttatttt
    27301 gttcctaaat atgtacccgc atcgattatc ccagacccta gagaaagcaa aatttctgag
    27361 gatcatttaa tcgaatcgat gaaaaaaacg gatgactctt atctgttgtt cgatactgaa
    27421 ccgacgccgt atcaagaaaa aagaaatggt gagccgtctg tacgaacatt caatcgaaca
    27481 aaaatgttag aggaaacaaa gcagtcagaa gaaaaaaaat cgaaaaaaag ccatggtatt
    27541 ttagaacgct cattacaagg attgatcgat gatcaaggaa actctttggg agaaaatagc
    27601 tattttaaat aagtgatata caaatttata ttcggctaca gttggaaagg aaacgctgcg
    27661 gcgtttcctt attttatact ctatccggaa gtttatatcg tatcctcaga tttttttgag
    27721 cttaagcgaa ttttttctcc caataagtaa gaaaataatg gcaaaataaa aaaacaaact
    27781 attttcgcag atcgtttgca aaaatagtt
//
LOCUS       DAMEET010000034        26891 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.034, whole genome shotgun sequence.
ACCESSION   DAMEET010000034 DAMEET010000000
VERSION     DAMEET010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 26891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.034"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(40..1146)
                     /locus_tag="PB780_001273"
     CDS             complement(40..1146)
                     /locus_tag="PB780_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDE0733337.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRTRYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            1356..2357
                     /gene="ccpA"
                     /locus_tag="PB780_001274"
     CDS             1356..2357
                     /gene="ccpA"
                     /locus_tag="PB780_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HDE0733338.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLADRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEGFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(2694..5039)
                     /locus_tag="PB780_001275"
     CDS             complement(2694..5039)
                     /locus_tag="PB780_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDE0733339.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            5318..6220
                     /locus_tag="PB780_001276"
     CDS             5318..6220
                     /locus_tag="PB780_001276"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDE0733340.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            6433..8208
                     /locus_tag="PB780_001277"
     CDS             6433..8208
                     /locus_tag="PB780_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HDE0733341.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(8274..9860)
                     /locus_tag="PB780_001278"
     CDS             complement(8274..9860)
                     /locus_tag="PB780_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDE0733342.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQEMVEEIE"
     gene            10111..10335
                     /locus_tag="PB780_001279"
     CDS             10111..10335
                     /locus_tag="PB780_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HDE0733343.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            10476..12227
                     /locus_tag="PB780_001280"
     CDS             10476..12227
                     /locus_tag="PB780_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDE0733344.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSVKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIISAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            12227..14011
                     /locus_tag="PB780_001281"
     CDS             12227..14011
                     /locus_tag="PB780_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDE0733345.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            14337..15188
                     /locus_tag="PB780_001282"
     CDS             14337..15188
                     /locus_tag="PB780_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDE0733346.1"
                     /translation="MLIEKVLNNNVVITRDERDREMIVMGKGLAFKKKVGDPILDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVHLNDSVYISLTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GAKSEDGEVSEELFQVVQKKYKDTFGCVEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            15285..17165
                     /locus_tag="PB780_001283"
     CDS             15285..17165
                     /locus_tag="PB780_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDE0733347.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEVEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVPFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTEPAIYGITLPKK
                     KPFIYSMIGGAISGIYLMASGTISYTMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDAIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(17205..18107)
                     /locus_tag="PB780_001284"
     CDS             complement(17205..18107)
                     /locus_tag="PB780_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDE0733348.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKYLLLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENKVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            18338..19741
                     /locus_tag="PB780_001285"
     CDS             18338..19741
                     /locus_tag="PB780_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDE0733349.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALNDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            19741..21174
                     /locus_tag="PB780_001286"
     CDS             19741..21174
                     /locus_tag="PB780_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDE0733350.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQVKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            21319..22245
                     /gene="trxB"
                     /locus_tag="PB780_001287"
     CDS             21319..22245
                     /gene="trxB"
                     /locus_tag="PB780_001287"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDE0733351.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            22459..24186
                     /locus_tag="PB780_001288"
     CDS             22459..24186
                     /locus_tag="PB780_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDE0733352.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEEAKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            24201..24551
                     /locus_tag="PB780_001289"
     CDS             24201..24551
                     /locus_tag="PB780_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDE0733353.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(24643..25503)
                     /locus_tag="PB780_001290"
     CDS             complement(24643..25503)
                     /locus_tag="PB780_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDE0733354.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLKQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            25595..26023
                     /locus_tag="PB780_001291"
     CDS             25595..26023
                     /locus_tag="PB780_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDE0733355.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            26185..26361
                     /locus_tag="PB780_001292"
     CDS             26185..26361
                     /locus_tag="PB780_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDE0733356.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            26388..26834
                     /locus_tag="PB780_001293"
     CDS             26388..26834
                     /locus_tag="PB780_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HDE0733357.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         8506 a         4796 c         5746 g         7843 t
ORIGIN      
        1 aaaaaggtgg gagcggacta gctctcacct tttttactct tatttttcta aaacaagtaa
       61 ttcttttggt gtagtcgtga atggcacaca gccatcgctt gtcacatata cacagtcttc
      121 gatacgtacc cctacttttt caggaatata gattcctggt tcaagagaga agcacatccc
      181 ttcttcgatt actaagtcat ttccttctac aagggaaggg aattcgtgga cggttgttcc
      241 gatcccgtga cctaaacggt gattgaaata ttcgccgtag ccatagctgt tgatcacgtt
      301 tcgtgcgatt tgatcaagtt cacctgctgt cacacctgga cggacggctt cttgtgcggc
      361 aagctgtgct tcaagagtga tgttgtagat tttttcttgg aagtcggttg gtttttgata
      421 agcaattgta cgagtagcat cactgcaata gccattccaa acaacgccaa gatcgaataa
      481 cacaagttca ttcggtgaaa tcttcgtatt tcctggtgta ccatgagggc ttgcagcatt
      541 tgctcctgca aggactaatg tgtcgaaaga catttgtgaa acaccttgtt ttttcaattg
      601 gtattcaatt tctgcaatga tcgcttgttc agtcacgcct tctttcactg ctttaaagcc
      661 aatctcaaat gcaacatcgg cccatttacc agcttcgatc agtttgtctt tttcctctag
      721 agttttgatc agttgtagac gttggatgat ggaagttaga tcagtgtaag aatccgttcc
      781 cgtcaagata tcagagatag catcgaagcg ctcgacagtc aaggcgttct tttcaatccc
      841 tacattgcca ggctgaccat agcgagtacg aagttctttt ccgatgatct cccaagggtt
      901 ttcgctgtct aaatagccgc ggacatcata cgcccaacta ctgttttgag catcttcaac
      961 ttccaaagca ggagtgaaaa ggaaaggatc ttgttcaaga gggataaaaa gagctagtac
     1021 acgctcatga ggatcacttt cgtatccgga aaagtaagcg atgtggccag ggtcgctgat
     1081 ataagccaat tcgacttggt gttgtgccat ccattgacgt agttcattga tttttacgtt
     1141 attcatttgg caacctactt tctaaattaa ttttgtattc aataattgta ccactgattt
     1201 attaaaagac caacgttcta aaaactgaaa aaagtaaaaa ttttcatgaa aacagagata
     1261 aaacggttgc aattcttgag attatttgat attctatttt agaaatgtaa gacgtatttt
     1321 cagaaaacat ataaaatatg aatgggagaa cacaaatgga aaagcaaaca atcactatct
     1381 atgatgtagc gagagaggca aatgtttcga tggctactgt ttcacgtgtc gtaaatggca
     1441 atccaaacgt taaaccagca acaagaaaaa aagtcttgga agtcatcgat cgcttggatt
     1501 atcgtccaaa cgcagttgcg cgtggcttgg caagtaagaa aacaacaaca gttggggtaa
     1561 tcatccctga tgtcagcaat atgttctttg cttctttagc acgtggtatc gatgatgtgg
     1621 caacaatgta caaatacaat attatcttag caaattcaga tggcaacgac caaaaagaag
     1681 tcaatgtatt gaataactta ctggctaagc aagtggacgg cgtgatcttc atgggtcacc
     1741 atatcacaga cgaaatccgt ggagaatttt cacgttcaaa aactcctgtt gtattagctg
     1801 gctctattga tccagatgaa caagtaggta gcgtaaacat tgattatgta tccgcaacaa
     1861 aagatgcagt aaataaattg gcaaaaagcg gcaataaaaa aatcgctttt gtcagtggtg
     1921 cgttgatcga tcctatcaat ggacaaaatc gtctgaaagg ctataaagaa gcattaagcg
     1981 aaaataacct accatatagc gaaggattga tctttgaagc acaatacaac ttcaaagatg
     2041 gtctatctct agccgatcgg atccataata gcggtgcaac agctgtctat gtttcagatg
     2101 atgaattagc catcggtatt ttggatggtt tattagatcg tggcgtgaaa gtaccggaag
     2161 gattcgaaat catcacaagt aacaattcac tactgacaga agtagctcgt cctcgtttaa
     2221 caagtatcac acaaccatta tacgatttag gggcggtatc tatgcgccta ttgacgaaaa
     2281 tgatgaataa agaagaagtc gaagaaaaaa cgattatctt gccttatgga atcgaagaaa
     2341 aaggatctac aaaataataa aaggacaacc attcagaggt tgtgagcctg ccgtttaact
     2401 ctgcgttaca aggagcaatt tcaaaaaaca gcatttcatg tttgatgaat attgtgactc
     2461 gtgattcgga gttggcagga gaacaccgtt tattcgggaa aaggggttat gacaaaattt
     2521 tgttataatc ctttttttgt gtcttaaaaa agagagaaag tggttgaaat tacatttatc
     2581 gtatataaaa caatccgtgt atagatgctg cattttgttc accaccatca aaaaaataag
     2641 ggactgtgac aatactattg tcacaatctc ttattactgt gtattctatt aaatcaatca
     2701 tctttctcat ctgattcttt ggttgcttga ttcgctcttg cataagctga agcggtgttc
     2761 cagtaatctg tatagttttc atcggtaccg ccaacgccaa atttaaaggc gcttttcttc
     2821 ggtccatttt tcgcccatag cgattcaact gtttttccag gtgtttggat ggatttttta
     2881 ttgacagtaa cttttccagg tgtttgacca gtaaatccag atactttttt cttcatcaca
     2941 tcagatgata attcgaattt ctcatcagtg ccaaaaatgt caggttttgc ttggtaagct
     3001 tgattgatca gattggcaag ataattagcc gaacgtatcc ttgctttgct gtccattcca
     3061 gtattgtcat cgtgccctgc ccagctactg atggtaatag acggagtaga gacaattagc
     3121 catgaatctc ggtattcatt ggtagaacca gtttttccga cccaatctgc ttttcctaga
     3181 tttccgttta agcttgagat ggcatctttg aatggtgtag tgatttttgc attgatcaca
     3241 ctgcgcatca tgtcattcat gatcgatgcc gttgcttccg aatagatccg gacagagttg
     3301 ctttcgtgtt tataaattac atttcctgcg ttatctgtga tggaatcgat gatatagcct
     3361 tcttcataga cgccgccgtt tgctaaagcc tgaaatccat tggtctgttg aagcgtcgtg
     3421 acatctactg tacctaaagg agcagattca acgccccaat tgtcatttgc cgggtagttc
     3481 atctttgcca agtagtgctc gtaagcatat tgtttcgatc caccggaatt caacacatct
     3541 tgatataagt gataagccgg gatattgttg gaccattcta gtgactcacg gacggtttgg
     3601 aaggtgttcg atccttcgtt agtagcattg acgattttct cgccagcatt ttctccttct
     3661 tgccaagttg ttgcataatc tgatacacgg gattctgatc cgataagccc catgtcgatt
     3721 gcaggtccat aaacaagaac tggtttgatt gatgagccag cttgacgttc tgtatcgaag
     3781 gcatggttat tttgattctg actgtaattc cgtccgccaa cgaatccgta gatgcggcca
     3841 gttctgttat ccatcaatac atttccggtt tctacttgac tgccagcacc atcatctaac
     3901 atgtatccat aattttctac gccattttgc aatgcagcat agacatcttt atcgattgtc
     3961 gaatggattg tatatccttt attttggatt gtttgctgtg ccttttgata ataagcgtca
     4021 ctgaccgatg aatcagacat atcagcgttg tctttttcag ccaattgttg tgcaatgatt
     4081 cttgttgctt cattcatcac agtataatac aagaattctc tgtcagtctg ttctgcgatt
     4141 tgttgtggta gaaagtcttt tgtaaggtca taggcttttg cttcttcgta ttgttctttc
     4201 gtaatttggc cttcacgata catactgaat aaaacaaaat cttttctggc aaggccggca
     4261 gaaagatctt cttttagcgc acctgtgttc gtgtatggac tatacgtgat cgggctttgg
     4321 ggtaatccag cgatataagc agcttgcggc agcgtcacat cttttgcatc tacaccaaag
     4381 attcctcttg cagcttcctg gactccagca atgttttgtc ccttattatt acgaccaaat
     4441 ggtgagacat tcaaatacgt agcaatgatc tcatcttttg aaaagtattt ttctacttgt
     4501 gcagaaagaa tgatctcgtt tgctttacgt ttgaatgttg tttcatctgt aaggatctgc
     4561 tgtttgacta attgctgtgt caaagttgat ccaccagaag agccaatacc agtagcttca
     4621 gatactaacg ctcgtaaaac agctttagga acgtaacctt ggtgtttatc gaagtattca
     4681 tcctctgtgc tgataattgc agttttcaat aaaggtgaaa ttttatcaga tgaaatagtc
     4741 gtgcgcatca gatctgtctg gatttttgat atctctgtat tatctgcata aacaatcttt
     4801 gacgtttctg taatgtttcc tagttctgtc tgcagtgctt ttttagtcgg caattgtgtg
     4861 tcttcgacga gataggcaaa atagccagcg cctattccaa tacctagagc tgcgaacatc
     4921 aaaagaagaa cagtaataaa aacagtcaat gattgaaaaa ctctaaaaat aatcttagga
     4981 acaaaccatt tttctttttt tcttctattc tgtttttttc gagtattttt gcgtggcaaa
     5041 agggtcacct caactaatgg ttttatttcg ctaattatag caaataatag aacaaaaaaa
     5101 cagacaaggc tgtttttggc aggaatttct tttttaaagg agttaaaacg tgagaaaatg
     5161 atctttttta taaaagagtt cttaagcttt tctttagaga aaatttcagt tactatggag
     5221 aacagggcaa agaaaaatca atcattttct ttgtatatca tcttaccaat atataggaat
     5281 tgttagtaac aattgccttt caaggaggaa tccatccatg ttattaggct cacacgtcag
     5341 catgagcgga aagaaaatgc tgttaggagc agcagaagaa gcagccagtt atggatcttc
     5401 gacttttatg atctatacag gcgcccctca aaacactaga agaaaagcca tcgaagagat
     5461 gaatattgat gccggaaaag cgtacatggc agaaaacggt ctaacaaata tcgtcgttca
     5521 tgccccttat atcattaatt tggggaatac gatcaaacca gaaatgtttc cttttgccgt
     5581 ccaatttctg agagaagaga ttgttcgtgc ggaagcttta ggcgctactc aaattacact
     5641 tcacccaggt gctcatgtag gggcaggaac agaagccgga attgctagaa ttattgaagg
     5701 cttgaatgaa gttttgacaa aagaacagac ggcacaaatc gctttggaga caatggcagg
     5761 taaaggtacg gaaatcggtc gaacatttga ggaattagcg caaatcatcg atggagtaaa
     5821 attgaatgaa aaattatccg ttacttttga cacctgtcat acgaatgatg ccggttataa
     5881 tattaaagaa gacttttcag gtgtaatgga agagtttgat aagatcatcg ggttagatcg
     5941 gcttaaagta ctccatatca atgattctaa aaatccgcaa ggttcgcata aagaccgaca
     6001 cgccaatatt ggttttggaa caatcggatt cgacacgctg aataagatcg tccatgatcc
     6061 aatgtttgac aatatttcga aaatcttgga aactccctat gtcggcaaag ataaaaagaa
     6121 agcttacgca ccatatggaa aagaaatcgc catgttcaaa gcacaaatat tcgatccaga
     6181 agtgtttcct gagttattag cagaagaagt gatactctaa gattttctat ctgacagcag
     6241 caaaaaagga ccaggcacat tgccttgtcc ttttttgttt aagaaacaaa ggagtgcttt
     6301 ttgcagtctt tttcatcatg catcttctcc cccctctttt taaatttaga caaacagggt
     6361 atactggtag aaaagtaaaa aaacgaaaag tttcacaagc aagtggcaaa acgtgattgg
     6421 aaggacgaaa agatgctgaa cactttaaaa cccttgatca accaactaaa aaagaaacat
     6481 acatacttta ttcttggcat agccctatta tttacaatta ttcgtttgta tttatatagt
     6541 aaagcaatct atggaacaga catgtcggca acctatgatg accagctgct gattcgctat
     6601 gctcaaaata ttttggacgg taaatggcta ggggagtata cgactacaac actttccaaa
     6661 ggaatctcct acagcttatt tttagtactt gcacataaac tgcatctttc ttatgcagtt
     6721 ttgttcggca tgttcagtat ttttgccagt atattggttg cactcgcgtt taaaccgatc
     6781 gtgaagaaca agtatcttct tttaagtttc tatttttatt ttttgttttc tccgattagt
     6841 tttacacatg aatattccac tcgaacgtat cgtaatgccc tcgtgattcc ggctgttgag
     6901 atcattatcg cttgtttact agctatttat taccgcaaat acggttctag cagaaaattg
     6961 ctgccatggt ttttagggct ttctgtcgct tttcctttct tttggttcat tcgcgaagat
     7021 tctatctggc ttcttccttt tactgtagta gctttgggta tcagtttcgt ccagatcttg
     7081 ctagggcaag acttttcttt caaagaagta aagaagttaa aaaaacgact ctttcttctc
     7141 tcgaaacggg aatggctgaa actttcattg tttttacttc ctatagttgg cttttccatc
     7201 gtctatcagt ggatcgaata taaaaatgag acaacatacg gtattgcaac catcaatgac
     7261 cgttccagtg gagaatttgg acgtttgatg aaacaactga tccgaataga tgatgaaacg
     7321 aacttgaata aagaaaacag tgagatatgg gtctcacatg ctgcactaaa taaagcatta
     7381 gccgcatctc ctacattcca tactgtttct acccgtattg acgaattata tacgactcac
     7441 gcgtggactc aaggtggaaa agtaaaagaa ttacccggag atatcatctt ttgggcattg
     7501 cgagatgtgg taagcgaatc tggttattac caaaacaatg caaaagaaac caatgatttt
     7561 tggaaaaaag tcaaccaaga attagaagaa gcctatcaca agggaaccat caaaaagaaa
     7621 aaagagttct atctgatggg atcaggagat ggaaagacga tgagtgatct accaatcgta
     7681 ttcgatttcc ttcaatcagg atacgcttat gcatggcgat ataaagattt ccatcaagga
     7741 ggaaattata gtttcggaac gcaagaacaa gtcaaacagg ctcaagaatt attaaacctt
     7801 ccgctaatgg agaattggat cgatagtaat gaaccgagaa cacctaattt ccaattaaca
     7861 aaatcagcaa aagtcgcgaa tgtcatcata aaaatttacc aagttctggc tattcccgtg
     7921 ctgattgctt caatagcagg gatattactt atgatactag gaaccgtatt tgcaaaagct
     7981 gatcgtgcct attatagttc ctctttgatt gtggtcaccg gattaatact gacacaattg
     8041 gtttttcttt tcggtgtctc gtggttttgt tcttatgcgc cagaaaaaaa ggattatttt
     8101 ttaagtgtct acactggcgc aggcgtgccg ttaacgcaat tcatcgtagt aacaagcatg
     8161 ctagttttca gtaaattaaa tctgaaaaaa gctttatctt ctaaataaaa agacccttgg
     8221 tagctagatt tttaacctaa ctatcaaggg tctttgtgtg gaaaaatcag tttttattcg
     8281 atttcttcta ccatttcctg caaacgctgt tcatattttt tgtttccttt atataaatca
     8341 ccaatcgtcc atgccaacag gaggaggaca agaacaatga ttacataagc gatgtgatca
     8401 gccaatgcta gttgagcgga agtggcttgc tcgccgaaga aactttcgat ctgtcctggt
     8461 aatcctcgta agttcaagta agttagacta atcacagaga accagccaag aagttttacg
     8521 atgaatgaat ttttaaatcg gctacccatc tccgcttggc tgttggtcat catcagtaaa
     8581 gggatcatcg aaaacggcaa cgcaaaagcc aagaacacct gggattcatt cattaagtta
     8641 ttcaaagcgg tatgttcttc gatcgttcct tttgtacttg taaacaaaac acaaatcaat
     8701 acaggaatca cagaaagcaa tcgtgtgacc agtcttctta gccaaatcgg gatacgcata
     8761 tggatgaatc cttccatgat gacttgaccg gtcaacgtac cagtaatagt agagttctgg
     8821 ccagaagcaa gaagtgctac agcaaataaa gtggacaaga taccagattt tgctacagca
     8881 atcaagatac cattgctcat agctgatgga tcagataaag cttcatacag accaaaaaag
     8941 gaaggatctt gaacagaacc ggttttgaag acagcgacac ccataatcaa tagcagcgca
     9001 ttgactagaa aagccatcga caattggata ttagagtccc aagtagaaaa acgtacagct
     9061 tgtgctacat cttcttcgtc ttggtgatcg atcttccgag tttgtgaaac tgctgaatgt
     9121 agatataagt tatgtggcat gactgttgcg ccaataatcc ctaatgctcc agttagcgga
     9181 gtttcgccac caatgacagg tgagtcggag aatgtatggc tgttagggat aaagccagca
     9241 aataaactgt gccaatctgg gttagacaag ccaacttgat agacaaaaac gaataaaatg
     9301 acgaaaatca agcagacaac cagtgcttcg atcttacgaa acccgacttt tgtcagtagt
     9361 aagagaacga aaacatctaa tacagtaata aagacggcaa tcaccaatgg aatatcaaaa
     9421 agtaagtaga gggcgattgc tgcaccaatg acttctgcaa tatctgttgc cataatggct
     9481 aattcagtca agatccataa aatgacaccc aatgtcttac ttgtgcgtgc tcggattgct
     9541 tgggccaaat ccatttgggt cacgattcct aattttgctg ccatatactg taaaagcata
     9601 gcaatcagac tagacatcaa aataactgac atcaataaat attggaaatt ttgtcctccg
     9661 gtgatcgaag tagaccagtt acccgggtcc atatacccaa ctgctactaa cgcaccaggt
     9721 ccagagtaag caagaagtgt tttaaaaaaa cttttcccct ttggtacatc cactgttcca
     9781 tttacttcag ctaaagataa accatttgta tgttcaataa gtcgatgctt atgaaattgt
     9841 ttgttggatt gattattcaa gaaaaaacca cctttcctat ttatctacga ttacattcta
     9901 cacctcaaaa agaaaaaatt aaagaaaaag attcggtatg cctaattttt tttcgtggcg
     9961 tgtcttgcta gatgacgaaa gaaattttgt tctcctctaa aaaaaccact attttttgca
    10021 tgaaactaaa gaatacgcct ttcttttttc aataaacttt agtaaaataa gtcattgaga
    10081 gcaattcgga aatagaaagg agagatttat atgttatcaa caggtattgt cgtacttatt
    10141 gctattattg ctttgctaat cggcgcagta ggtggctttt tcctagcgcg taaatatatg
    10201 aaagactatt tagaaaaaaa tcctcccgtt aatgaggaca tgttgcgatc aatgatgatg
    10261 tcaatggggc aaaaaccatc tgagaagaaa attcgccaga tgatgcagca aatgaagaac
    10321 caagggaaaa aataggttct gacccaacga ctttttacgg gctgattttt tcagtccgtt
    10381 ttttatttcg taatttgtta gactattcag attattcagc aaaaagacaa gcacagacaa
    10441 tcctaggctc ttgataaaga caaaggagga gtaaaatgtc gatttttaga aagttaggct
    10501 ggttttttcg tcaggaaaag aaacattatt taattggggt tttctcgcta ttcgcagtgg
    10561 ccttggtaca gttagttcct ccaaaagtga ttgggatcat cattgatgag attgccgata
    10621 aaaatttatc agtaaagatc attttcgggt ggatcttcct attgattatc gctgcttttt
    10681 tacaatatct ttttcgttat atctggcgga cgaatatttg gggaagtgca gctcggttgg
    10741 agaaagaact tcggcagcag ttattcaaac attttacaga aatggacaat ctcttttacc
    10801 aaaaatatcg gactggggat ttgatggctc atgcaacgaa tgatttgaat gcgatccaga
    10861 atgtagcagg agctggtatc ttgacctttg cagattcagt gattacgggc ggaacaacga
    10921 tcattgcgat gattttgttt attgattggc gcttaacact gattgcgctg attcctctgc
    10981 ctttgctggc cgtcacttca cgagtgctgg ggtcgaaact ccatgatgct tttcgtgatt
    11041 cgcaagaagc tttttctagt atcaatgata agacacaaga aagtatcact gggatcaaag
    11101 tcattaaaac atttggacaa gaaaaagaag atctagcgga ttttgaagaa aagattgaag
    11161 atgcaattat taaaaacaaa cgagtcaatt ttttagactc cttatttgat ccattcatta
    11221 ccttgattat cggtctgtct tatgttgcaa caattatttt aggtggaaag tatgtcttgg
    11281 aaggcacgat ctcgatcggg cagatggtct catttattag ttatatcggt atgcttgttt
    11341 ggccgatgtt tgcgattggt cgattgttca acgtattgga aagaggaaat gcttcttatg
    11401 atcgcgtcaa tgaacttttg catgaaaaaa cgcatatcgt ggaacaactg ggtgcagtgc
    11461 agaccccggc aaaaggcgag ctgaatgtag ctatttcctc ttttaattat ccgaaagaag
    11521 aatcaccagc attagaacaa atcaagtttg caatccaaga aggagaaaca ttaggtattg
    11581 ttgggaaaac tggtgcggga aaaacgacga tcatcaaatt actgatgcgt gaatacgatg
    11641 attatcaagg gaaaatccaa tttggcggac gttcgatcaa agaatattca ttgaatgctt
    11701 tattaggatc aatcggctat gtgcctcagg atcacttctt gttttcgatg accattcgag
    11761 ataatatccg tttttctaat ccaaaacttt cagaagaaga agtagagcat gcggcttctt
    11821 tagcatttat caacaatgaa atcaaggaat taccaaaagg ttatgatacc ttagttggag
    11881 aacgtggagt ctctttatct ggcggacaga aacaaaggat ttcgattgct cgagcattga
    11941 tcaaaaatcc tgaattatta atattagatg atgcactttc tgcagtagat gccaaaacag
    12001 aagaagctat cttgcataat ttaaaagaaa taagaaaaga gaaaacaact atcatttcag
    12061 ctcaccgttt aagtagcgtg atgcatgcaa aagaaatcat tgttgtcgat caagggaaaa
    12121 tcattgaacg aggaacccat catgagctgc ttgcactagg cggctggtat gaacaaatgt
    12181 gggagaaaca gcaattagaa gcgaagatag aaggaggcga ggcctgatgg atgaaaatta
    12241 tcaatcggaa tggacaaaat cgattccact taaagaacag aaaaagatca tcaaacgact
    12301 gatgaagttc gccaaacctt ttagaagaac gttcatcgtt gctatcttat ttgcgtttgc
    12361 tctgtctgtg atcaatgtcc tattgccaag gatcatccaa acttttatgg atgatcatct
    12421 ggctaaacag tcggctacga cacaagttat tttgtttttt gctggtatgt acttatttgg
    12481 agtcatagta aaaagtatca tttggttttt ccaatggtat ttgtattcca tggcttcatt
    12541 gaagacctat cagtatatta gggtaaaatt atttgaaaag ctgcatacac taggtatgcg
    12601 ttattttgat cagacaccgg caggatcaac cgtttctcga gtgacgaatg acacagaaac
    12661 gctatttgaa ttttggtacg tctttttgat ggtattgact gggatctttg cagtcgtttc
    12721 ttcattcatt gccatgtttc aaatcaatgg agaaattgcc ttgtggactt tggtgttctt
    12781 gccaatttta ggaatagtca tctggtatta ccagaaattt agttctcgta tttaccggaa
    12841 tatgcgggaa aaattaagtc agctgaatac gaaattgaat gaatcgattt ccggcatgcg
    12901 tattatccag cagtttcgcc aagaagctcg tttagctaaa gaatttgaac aagtgaacaa
    12961 tgagtattta gacacgcgtt atgcgatgat caaaacaaat tcgattctgt taagtccgat
    13021 catcaatttt ctatatgcat tggcgattgc gttggtgttg actttgtttg ggatcgatgc
    13081 gttgcattct cccgtagaag ttggactgat ctatgcattt acgacctatg tccagggatt
    13141 ttttaatcca atgtctcaaa tgatggactt tttgagcatc ttcacagatg ggatggttgc
    13201 tggtagtcgt atcttgaaga ttatggatac agaagaatat gcaccacaac aaaaccctgg
    13261 agcagatcaa atgattactt caggtaagat tgagtttcgc aatgtaagct tttcttatga
    13321 tggagagcac aatgtattaa acaatatctc ttttgttgct aatccgggag agaccgttgc
    13381 attagtggga cacaccggaa gtgggaaaag ctcgatcatc aatgtgttga tgcggtttta
    13441 cgaattttat gaaggtcaga tattgattga tgatcatgac attcgagaat atccaatgga
    13501 agagttacgt aagaaaatgg ggctggtctt gcaagatgct ttcttgtttt atggggatat
    13561 cgcaggaaat attcggatga tgaatccagc tatcacagac gaacagatac gccatgcggc
    13621 tgagtttgtt caagcaaaca aattcatcga tgaattacca aaaggatatc actcaaaagt
    13681 gatcgaacat ggtgccagct attctagtgg acaacgacag ctgatcactt ttgcgcgaac
    13741 aatcgtgact gatcctaaaa ttctagtatt ggatgaagca acagcaaata tcgatactga
    13801 aacagaagga ttgatccaag aaggattggc gaaaatgcga caaggacgga caacgattgc
    13861 gattgcccat cggctgtcaa cgattcgaga tgcagaattg attttagttt tagacaaagg
    13921 tcatatagta gaacgcggga atcatgaaac acttctagcg caaaatggtt tgtatgcaga
    13981 tatgtacaaa ctgcaaagca gtggtatata aaagatgttg tgacagtagc attttgacct
    14041 aagcactaga gtgaaaataa aacatagctt cccatatttt tcatctgtga gagttagtgt
    14101 cgtagttacc agatttaaaa aagacgacgt cagctagtca gaagtgccta atagccccta
    14161 agaaagcgtt gacaaaatcg tttgctttcg ataatataaa gaaaacgata caacttggat
    14221 agtgattgcc tgatgcaagc taaacctaat gagtctccca tggtttcatg gacggctttt
    14281 tgggtttttc tttttgtttt ggatacagcc ctatatatca tggaaaggag caaaacatgc
    14341 tgattgagaa agtactgaat aacaacgtcg taataacaag ggatgaaaga gatcgggaaa
    14401 tgattgtgat gggaaaagga ttggcattca aaaagaaggt gggtgacccg attcttgatt
    14461 catgcatcga caaaaaattt cagttgacaa atgacggact ttcacaacag ttccaagaat
    14521 tggcacaagc gattcctctt gagtacttag aaatttcttg tgaaatcatt gaatatacaa
    14581 aagaggctct gcaagtccat ttaaatgatt ctgtgtatat ttcattgacg gatcacttgt
    14641 atacagctat cgaacgtcaa aaaatgggga tctttgtgcc aaatgtattg ctgtatgata
    14701 tcaaacaact gtttcctaaa gagtatgcca ttgggaaaaa aacagtgaag aaaatctcgg
    14761 ataaatacca catcaagtta tcagaagacg aagccggatt tatcgcattg catttagtca
    14821 atgcacaagg cattcaaagc cagcaagaca atacctacct gtttaccaca acaatacagg
    14881 atattttaaa tatcgtccgc tattactata agattacatt tgatgaagat tcagtccact
    14941 tttttcgatt cgtcacacac ctgcgttttt ttctgacacg tgttctagat ggagcgaaat
    15001 cagaagatgg agaagtttct gaggaattgt tccaagttgt tcaaaaaaaa tataaggata
    15061 cgtttggttg cgttgagaaa atagcagact ttttaaccgg ggctttcgac taccagatgt
    15121 ccacggatga aaaaatgtat ttaacgatcc atactgcgcg tttagtgcat gagaatcgtt
    15181 gtaaataaat aaaaaaggag gacaaattaa caagaacgta tgaaaataag ggtagtgaca
    15241 ttcagttggc taaaccttcc actaattaat tggagggaat agaaatggga aaataccagg
    15301 aattagcaga aaacatcgta aagaatatcg gaggaaaaga aaatatccgt agcttgacac
    15361 actgtgtgac gcgacttcgt ttcaaactaa aggaggaaaa taaagcaaag gatgatgttt
    15421 tgaaaaagat ggaagggata gtcaccatca tgaaaagcgg cgggcaatat caagttgtaa
    15481 tcgggaatca tgtgccagat gtctatgcag aagtactgac agtagccggg atttccgatg
    15541 aagtagaaga agaggtttct tcaagtagta atctgttcaa tcgattaatc gacatattaa
    15601 gtggctgttt ccagcctttt ttaggtgctt tagctgcagc gggaatggtc aaaggattaa
    15661 atgctttgtt cttgtttctc aaattttata ctgaaggatc gggtacgtac atcatgctga
    15721 atgggatagg ggatgcaatt ttttatttca tgccagtgat cttagggtac acagcagcga
    15781 aaaaatttca tctgaatccg atggtggggc tcgtgattgg tgcagcactt tgttatccga
    15841 ctgtacaagg agcagcacta caaagtggtt tccctgatgg cagtcctgcg ccttatagct
    15901 tgctgggtct tccggcttat gaaacatttt tgggtatccc atgggtgggc agcagttata
    15961 ccagtagcgt cgtacctgtt atcttcatta tcgcatttgc cggacaagtc caaaaaattg
    16021 ccaagaaatg gattcctgca gtcgtccaga cctttttggt tcctttttct gtgttattaa
    16081 tcgcacttcc tgctggcttt ttgattatcg gtccgattgt cagtatactg acagatctat
    16141 taagcacggg attccaagca ttgatggact tttctccagc tgtgtatggt gcagttctag
    16201 ggtttctttg gcaagtactg gtgattttcg gacttcattg gagtattatc cctcttgcga
    16261 ttatgcagct agcgcaaaat gggtggagtc agatgttagt aggcgtttat ggcgcgagtt
    16321 ttgcacagac agcagtggta ttggcgatgt atttcaagtt caaggatcaa aaaatgaagg
    16381 cgctttgcat gccagctgtg atttcaggaa tcttcggcgt aacagaaccg gctatttatg
    16441 gaatcacctt accgaagaag aagccattta tttactcgat gattggtggt gccatttcag
    16501 gaatctattt gatggcatct ggaacaatca gctacacaat gggcggatta gggatttttg
    16561 gggtggtaaa ctacatcacg gaaacagacg ccagtggtat gtttcaagcg tttatcggca
    16621 ttgctattgc ttcggtaatc gggttttcgc tgactttttt cttttggaag gatgaagcag
    16681 gtgaaacaga tgctatacag aaaaaatcag gtaaagaaat tgtaaaaaca ccgatacaag
    16741 gacaaatcgc tccactaaat gcagcgaagg atgcagcttt tgcacaagga acactaggtc
    16801 gaggaatctt gatctaccct gaaaaaggag aagtacgtgc cccttttgac ggaacagtca
    16861 tgaccctgtt cccgacgaaa catgcaattg gaatggtttc tgaaacaggg ctggaattgc
    16921 tgattcatgt cggattggat actgtgcagt tggaagggaa atattttgaa agcttagtgc
    16981 agcaaggaga aagagtgaaa caaggcgatc tgttgctacg cttcgacaaa aagaaaatcg
    17041 aaaaggaagg atacagttta gaaacacctg tgatcgtcac caattacacg gattatttgg
    17101 atattttgga aaaccaaggg gagacggtcg ggccagacga tgcacttatc accgcactga
    17161 cctgaacggt taggtgaaga atggaatagg cttaactatt ctttttattc cacttcttta
    17221 ttttcaaaat tgaaaggctc tttcatgatg gctgagctga tagggcgttt atcgaatttt
    17281 tgaccgacag aaattagatg gactaaattc tgttgataat tttctgcaaa aacacaacta
    17341 tataacacgt ctagaagata acaaattgat gtattgatcg taaaattacc aatttttgaa
    17401 tacagtcgct cgcgtgtcgt aagtcgcaaa gcggctgtac tcattgcaga tagcgttgaa
    17461 tcaccgatac tggtcaaagc taaaattgga acatgatttt gtttcaatat ttctgcggta
    17521 cgaagaatca ctttattttc acccgtataa gaaatcagga tcccgcacat atcgctcgtg
    17581 caattgtacg cttcatagaa tgattcacca aaagtggtga caattgtgac atccattttg
    17641 atgcgcttca ttttcagcat gaaatcttga gaaatcaaag tattcgagtt actggcgaag
    17701 atcacgattt tttttgcctg gagcagtaaa tacttggctt tttgcagttg gtcatgatga
    17761 atgagagaaa gtgtatcttg aatcgtcatc tgttctaaag aagccatttt atttgcgatc
    17821 gtcataatac cgtctgtggt ggaaaaagga agattcgcat ctacgtcttg aaaattccct
    17881 tcaagatagg ctgtttcagc taaaaaagca ttacgaaaac tgacccagcc atcaaaaccc
    17941 attttttttg ctacacggat gagtgtagac gggtggacat agcatcgctc agcgatctct
    18001 tggatggtca tttcgccaag tgctggagaa tcgcttaaga gaaactggat cacttcattt
    18061 tctgaaggag aaaaggagat ttctttgatt ttttcacgta ataacatgta gttaccttct
    18121 tcttattttt ttacgtaaat tataactttt ttgaaaatgt tttgtaaacg gatttatact
    18181 atataactta aaatagactt tttttgacaa gatatttaca aaaatccact agaaaacagt
    18241 aacttttgta aatgttactg gaaagaaacg gatttctatt gtttgttttg tttacaaaaa
    18301 agataatgaa agtgtaaaaa agtaaaggag tgagaacatg gcaaaaggaa tcaaaatcgc
    18361 tacgattggt ggaggcagca gttacacccc tgagttgatg gaaggattta tcaaaagata
    18421 tgaggaatta ccgatacgag aaatctggct cgtagatgta gaagaaggaa aagaaaagct
    18481 tgagatcgtt agtcagatgt ctcagcgtat gtgggatgct tctccgtatg aagtcaaaat
    18541 ctatgcaact ctggatcggg aagaagcatt gaatgatgcg gattttgtta cgactcagtt
    18601 tcgtgtcggt caattagatg cacgaatcaa agacgagcgg atccctagtt attatgggat
    18661 gattgggcaa gaaacaaatg gtgctggcgg gatattcaaa gcttttcgta ccgttccagt
    18721 ggtcttggca atcgttgaag acatgaagcg tttatgtcca aatgcttggc tgatcaattt
    18781 cgcgaaccca agtgggatgg tgacagaagc agttatcaga tatggcaaat gggataaagt
    18841 gattggatta tgcaatgtgc cagtaatggc gatgatgaca gaaccagcaa tgcttggcaa
    18901 gaaaaaagaa gagctgatct acaagtttgc cggtctaaat catttccatt ggcacaaagt
    18961 agcagataat aaaggaaatg accgcacggc agaattgatt gatcgtttat acgatgaaga
    19021 caatggattg ccgaaaaata tctttgatat cccgtttttc aaagaacaat taaaacaaat
    19081 gaatatgatc ccttgtggct accaccgcta ttactaccgt gaagaagaaa tgctggaaca
    19141 tgctttagaa gaatatcaaa cgattggtac aagagcgcag caagtgaaag aaacagaagc
    19201 agagctattt gaattataca aagatccaga attagaccat aaacctgaac aattgcaaaa
    19261 acgaggtggc gcttattatt cagacgctgc ctgtgaaacg attgcttcaa tctatggaaa
    19321 taaagaaaca cagatcgtgg tatctacaaa gaatgatgga gctgttcctg acctgccggc
    19381 agattgtgta gtagaagtca ctgcctatgt tggcgcacaa ggagcgagaa atgtagcgtt
    19441 tggttctctt cctgctgctg aaagaggctg gttgcaagta atgaagaata tggaattgct
    19501 gacgatcgaa gcagctgtga ctggtgatta tgggacagct ttacaggcgt ttacgatcaa
    19561 tccgcttata cgatcaggaa agacagcaaa acggatcatg gatgagttgt ttattgcaca
    19621 ccagcagtat ttaccgcaat tcaaggaaac aatcagccga ttaaaagaag aaggaatcga
    19681 agttcaagat gaactggcaa gagaattatc aggtaataca catataatgg aggaagtata
    19741 atgggattca gaaaagattt tttatggggc ggtgctacag cagccaatca atgtgagggc
    19801 ggttatgatg aaggtggacg cggactagca aacgtagatg ttgtgccaat cggaaaagac
    19861 cgttttcctg ttatcaccgg aaagaaaaaa atgtttgaat ttgatgagga tcatttttat
    19921 ccagcaaaaa ttgcaatcga tatgtatcat cgttataaag aagatattgc tttgtttgca
    19981 gaaatggggt tcaagactta tcgaatgtcc atcgcctgga gtcgtatttt tccaatgggg
    20041 gatgaaaaag agccgaatga agctggcttg aaattttatg aggatgtctt taaagaatgc
    20101 caaaagcacg gaatcgagcc acttgtgacg attacgcatt ttgattgtcc aatgcatttg
    20161 atcaaagaat acggtgcgtg gcgttccaga gaactcgttg gtttttatga aaatctgtgt
    20221 cgagtcatct tcaatcgata taaaggactt gtcaaatact ggctgacatt caatgagatc
    20281 aatatgatcc ttcatgcacc attcatggga gcaggactgt actttgaaga aggagaaaac
    20341 gaagaacaag tcaaatatca agcggcacat catgagttgg ttgcaagtgc catcgcaacg
    20401 aagattgctc atgaagtaga tccggagaac aaagtaggct gtatgctggc agcaggagca
    20461 tattatccat acacttgcaa tcctgaagat gtttgggctt ctagaaaatc agatcgagaa
    20521 aattatttct tcattgatgt tcagtcacga ggagaatacc cagcttatgc attgaaagaa
    20581 atggaacgaa atggcattca gatcgaaatg gaaccagggg atgccgcatt gctgaaagaa
    20641 catactgttg attttatctc tttttcttat tattcttccc gagtttcgac taccgacaac
    20701 gaactcttgg aacagactgc aggaaatatc tttgcttctg tcaaaaatcc atatctgcaa
    20761 gcaagtgaat gggggtggca aattgatcca ttaggcttga gaataacgat gaatgatatt
    20821 tatgatcgtt atcaaaaacc attatttatt gtagaaaacg gcttaggtgc agtcgatacg
    20881 ccagatgaga atggttatgt ggaagatgat taccgaatcg attacctggc agcgcatatc
    20941 caggcgatga aggatgcagt agaactagac ggagtcgatc tattaggcta tacgacttgg
    21001 ggatgtattg atttagtatc agctggtacc ggtgagatga agaaaagata cggattcatt
    21061 tatgtcgatc gtgataacca aggaaatggc acattagccc gttcaaagaa aaaatcattc
    21121 aactggtata aaaaagtaat tgattcaaat ggagaaaatc tttcgaacga ttgatcaatg
    21181 atttataaaa gtctattgtt tttcagcgga tttttcgagt ataataaagc tattcgagaa
    21241 acgcaagcag aacaatagac tttttttcgc attggctgta aagtatgctt acttgattaa
    21301 gtaaaggaga atctacatat gtatgatgta atcgttattg gcgcaggtcc tgctgggatg
    21361 actgctgctt tatatgcttc acgttcaaac ttatccgtat tgatgatcga acaaggtgcc
    21421 cctggcggcc aaatgaacaa tacggcagaa atcgaaaatt atcctggatt cgattcgatc
    21481 atgggccccg atctagctta taagatgtat gaaagtgtct caagatttgg gacagaaaat
    21541 gcttatggaa tcgttcaaga tatcaaggat cacggcagcc ataaagaagt catctgtgaa
    21601 gataaaagtt acgaaggaaa aacagtcatt atcgcaacag gctgtatcca ccgtaaatta
    21661 ggcgtacctg gcgaggaaga atttgctgga cgaggcgttt cttactgtgc agtctgtgat
    21721 ggtgcgtttt tccgtaacaa acgtctagta gtcatcgggg gtggggactc agcagtagaa
    21781 gaagccatct atttgacaca atttgcttct gaagtagtga tcgtccaccg tagagatgaa
    21841 ttacgtgcac aaaagatcat ccaagaccgt gcatttgcaa atgaaaaaat ttcctttatg
    21901 tgggatacag tcgttgacga aatcgttgga aacgaaatgg tagtgacagg tgtgaaagca
    21961 cgtaatgtca aaaccggcaa tgaatttgat ttagcagcag acggagtatt catctatgtt
    22021 ggactggatc cattaactgc accttttaga gaagcaggtt tgacgaacga agctggctgg
    22081 attccaacag acaacgaaat gaaaacgaat atgccaggcg tttttgccat cggagatgtt
    22141 cgtgaaaaag atttgcgaca aatcactaca gctgttggtg acggcggtat tgctggacaa
    22201 caagtgtata aatacttgga atctttagaa gaaacaactg cataagttta aaaagagtag
    22261 aagaacgggt tcaattgttc ttttactctt ttttttcgcg tgaaatttac tgaatctgat
    22321 ttatgcgaac acgtccaaca ccttttgata aaagaatcct ttcttgattt aagcgttttt
    22381 tggtcctcat actttcatat ctaataagtg aatgctatac ttattaagaa ccatatttca
    22441 gaaagaggtg ttattttcat gtcttgggaa caagtgtatg aacaatgggt acatgaagaa
    22501 aatttagaag aaaacttaaa aaaacaatta aatgatctgg gtgaagatcc tgaaaaactt
    22561 gaagatgctt tttatgcacc gttggaattc ggtactgcag gcatgcgggg aatcttagga
    22621 cctgggatca atcgaatgaa catctatacg gtaagacaag caacagaagg actagcacgt
    22681 ttcatggata cacaaggtga agaagcaaaa cgccgaggtg tcgctattgc ttatgactca
    22741 cgccatcagt caccagaatt tgcaatggaa gcagccaaaa cactggcaca tcacaatatc
    22801 ccgtcttatg tattcgaaag tctgcgtcca acaccagagc tttcttttgc agttcgtcat
    22861 ttgaagacgt ttgcgggaat catggtaact gcatctcaca acccagcggc ttacaatgga
    22921 tataaagtct acggagaaga tggcggacaa atgcctcctg cagacgcaga tgcattgacg
    22981 aaatatgtcc gtgaagtcag caatccgcta aaagtcgaag tattatctga cgaagaagct
    23041 aaacatagcg ggttgatcac gatcatcgga gaagaagtgg acgctgctta tctagaagaa
    23101 atcaaagcag tgacgattga tcgtgaattg gtagaaacaa tggggaaaga tttgaaactt
    23161 gtgtacacgc cacttcatgg aacagggaaa atgttgggcg aacgtgcgtt gaaacaagct
    23221 ggattcgaac aattcgtact agttcccgaa caagctgtgg cagatccaga tttcactacg
    23281 gtcaaatcgc caaatccaga agaacattca gcttttgaat atgctatacg acttggagaa
    23341 aaagaaggag ctgacttgct gattgcaaca gatcctgatg cagaccgttt aggcgcagcg
    23401 gttcgtttgc cagatggttc ttatgaagta ttgactggga accaaatcgg ggcattgttt
    23461 gctcaatata ttttggaagc acatcaatca agaggcactt taccagaaaa cgcagcggta
    23521 ttgaaatcaa tcgtttccag cgaattgcct acagcaattg cggaacacta cggtgctacc
    23581 atgttcaatg tgttgacagg gtttaaattc attgctgaaa aaatccaaca attcgaagaa
    23641 gaccattcac atacttttat gtttggattc gaagaaagtt atggttattt agttaaacct
    23701 tttgttcgcg ataaagacgc gatccaagca ttgcttttat tagcagaagt agcagcttac
    23761 tataaaaaac aaggcaaaac gttgtatgac ggcttgcaaa cgatttttga aacctatggc
    23821 tattttgcag aaaaaacgat ttctgtgaca atgagcggac aagaaggttc tgcgaaaatc
    23881 tctgctttga tggaaaaatt ccgtaaagaa gcaccgacag aatttgccgg agcaaaagtt
    23941 attcaaacag aagactttaa agaattgacg agaacagacc gtgaaggaaa cgtagaaaaa
    24001 ctaacaacac cgccatccga cgtattgaaa tatacattgg aagaaaatgg ctggattgct
    24061 atccgccctt caggaactga gccgaaaatc aagttttata ttggtgtaaa aggaagctct
    24121 acagaagacg cagccgaaaa agtagcctct tatgaagcga ttatcaaaaa gatgacagaa
    24181 gaataaaaaa cttgagactg atggaaaaag tggtaaactt tagaagtcag tcttttttca
    24241 agttggaggt gaatggattg ccagacacgc aaaaatcaaa agtagagaaa gtcagtcaat
    24301 tattcaaaat gttgagtgat cccactcgtc ttaaaattct gctatatctg aaagacgggg
    24361 aacaaaatgt gacagcaatc acccaagcag tggaaatgga gcagtctgct gtatcgcatc
    24421 aactacgttt attaagagaa aatcatgtcg tcaagtctca tcgcgaggga aaagcgattt
    24481 tgtatagtct tgatgattct catgtactgg atattttgaa ccagacactc aaacatgtgg
    24541 aacatcaata acgataggtg caaatagata aacactggag tgtgacaacg aaatttcttc
    24601 gttgtcacac tccagtgttt tttaagaaaa agacaaaaat gattaatcgc ttttttctgt
    24661 tgtttcagtt ccataataac tatgatcttc taggtctaaa gcattcaaaa taagcgaagc
    24721 cgtttcttca atagataaag atgccacatt gatcactaca catcctagtt tttcataaag
    24781 atcattcgca aaatcgagtt cagctctaat tttttcgata tcggaataag cagtatcagg
    24841 gtttaatccg taagagcgca tccgttcttt acggatatta tttaaaatat ctgggttatt
    24901 cgttagtccc acgatttttt taggatcgat ctcccaaagc tgtttgggaa tatgtgcttg
    24961 tgggatcaaa ggtaaattgg caacttttaa atttttgtta gctaaaaaca agctcaaagg
    25021 cgttttggaa gttcgggata cgcctagcaa aacgacatct gcttctaaaa aaccacgagg
    25081 atctttccca tcatcgtatt ttacagcaaa ttccattgct ttgatccgtt tgaaatagtt
    25141 ctcattcaat aaatggaggg caccaggttg ccgaattggc ttgataccgg ttaaacgttc
    25201 aatttctgat atcggtggcg tcatcacatc aaaactaaat aattgatgtt cttcacaaaa
    25261 agaacgtgtt atctcgatca gttcttgatt gattaatgta tgcaggacaa gtgctttttc
    25321 ttctaatgca tcttgcaatg cctgctttaa tgttttttca tctgtcacga acgttctgcg
    25381 gaataggttg aactcaacgg taggatactg agccatagta gcttgcgcta gttttgtcgc
    25441 agtttctcca gcagagtcgg agatgacaaa aaaagtaacg atttctgtag aatcggaaga
    25501 catggataaa acctcacttg catttttttt atgtttatta taccatactc tagaatgcgt
    25561 acttcgtaat cattttgatt tggaggaata agtcatgaaa aaacagacat tgatcgaaca
    25621 atcattagaa acattgaaaa ataacggttt taaatatact aaaaaaagag aagcattgtt
    25681 aacttattta gtgaagcgta accgctacgt atctgctaaa gaagtttttg actatatgaa
    25741 tcaggaattc aaaggggtaa gttatgatac gatttatcgg aatctccatg attttgcaga
    25801 gatcgattta ctggaagaaa ccgatttaaa tggagaaatg aagtttcgtt ttcattgctg
    25861 tcagggggaa atcggccatc accatcattt tatctgtaca gtgtgcggaa caacgaaaga
    25921 aatccatatg tgtccgatga attatttcga agaacagcta ggtggatgca cgatcgaagg
    25981 gcatagattc gagattttcg gaagatgtga aaattgtcaa taagggttta aataaaataa
    26041 catttgataa ttttaaaaaa aagcaagatt tccttgacgg tcaaaacagc attagatata
    26101 atatttacag gacagtcttg ttttttgaga tatatttcaa taacaattac ggttactttt
    26161 taagctcgga gggagggaat taacatgtca aaaacagtcg ttcgcaaaaa cgaatcactt
    26221 gacgatgctc ttcgtcgctt caaacgttcc gtgtcaaaag cgggtacttt acaagaatct
    26281 cgtaaacgtg aattctacga aaaaccaagt gtaagacgca agaaaaaatc tgaagcagct
    26341 agaaaacgca aaaaattcta gttctgtgat gaattggagt tggtagtgtg tcactactaa
    26401 gtagattaaa cgatgacatg aaagcagcga tgaaagcgaa agataaggaa tctttacaag
    26461 tcatccgaat gatcaagtca tctatacaaa acgagcagat taaagaaggt cacgacttga
    26521 cagaagaaga agaactaacg gtgttgtctc gcgaaatgaa acagcgacgt gattctttgc
    26581 atgagtttga agaagcagga cgtgacgatt tagctgaaaa agtaaaaagt gaaattgtaa
    26641 tagtcgaaaa atatatgccg gaacaacttt ccgatgaaga aattcgacaa cttgttcaag
    26701 aagcaatcac tcaaactggt gcttcatcaa tgaaagaatt tggcaaagtt atgggtgcaa
    26761 ttatgcctaa agtcaaagga aaagcagatg gaaaccaagt gaatgcgatt gtgaaagaac
    26821 tattacaatc ttaattttat catttaaact gcggacagtg attgtccgca gttttttgtg
    26881 ttcgtaaaaa a
//
LOCUS       DAMEET010000035        26816 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.035, whole genome shotgun sequence.
ACCESSION   DAMEET010000035 DAMEET010000000
VERSION     DAMEET010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 26816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.035"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..411
                     /locus_tag="PB780_001294"
     CDS             <1..411
                     /locus_tag="PB780_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDE0733358.1"
                     /translation="GLYYSPVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAP
                     QEYLSRAYNDAPKYCANIVNTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLA
                     ELKGYQVTTGEPVCQTRRLNSRIDKVRYMFNKIT"
     gene            509..697
                     /locus_tag="PB780_001295"
     CDS             509..697
                     /locus_tag="PB780_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HDE0733359.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(755..1108)
                     /locus_tag="PB780_001296"
     CDS             complement(755..1108)
                     /locus_tag="PB780_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDE0733360.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            1639..2061
                     /locus_tag="PB780_001297"
     CDS             1639..2061
                     /locus_tag="PB780_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDE0733361.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2058..2288
                     /locus_tag="PB780_001298"
     CDS             2058..2288
                     /locus_tag="PB780_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDE0733362.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            2786..2986
                     /locus_tag="PB780_001299"
     CDS             2786..2986
                     /locus_tag="PB780_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDE0733363.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3013..4206
                     /locus_tag="PB780_001300"
     CDS             3013..4206
                     /locus_tag="PB780_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDE0733364.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(4274..5839)
                     /gene="guaA"
                     /locus_tag="PB780_001301"
     CDS             complement(4274..5839)
                     /gene="guaA"
                     /locus_tag="PB780_001301"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDE0733365.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(5898..6821)
                     /locus_tag="PB780_001302"
     CDS             complement(5898..6821)
                     /locus_tag="PB780_001302"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDE0733366.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7017..7622
                     /locus_tag="PB780_001303"
     CDS             7017..7622
                     /locus_tag="PB780_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDE0733367.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            7930..8946
                     /locus_tag="PB780_001304"
     CDS             7930..8946
                     /locus_tag="PB780_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDE0733368.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            9087..10388
                     /locus_tag="PB780_001305"
     CDS             9087..10388
                     /locus_tag="PB780_001305"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDE0733369.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            10434..11096
                     /gene="deoC"
                     /locus_tag="PB780_001306"
     CDS             10434..11096
                     /gene="deoC"
                     /locus_tag="PB780_001306"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDE0733370.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            11117..11515
                     /locus_tag="PB780_001307"
     CDS             11117..11515
                     /locus_tag="PB780_001307"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDE0733371.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            11581..12663
                     /locus_tag="PB780_001308"
     CDS             11581..12663
                     /locus_tag="PB780_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDE0733372.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            12875..14437
                     /locus_tag="PB780_001309"
     CDS             12875..14437
                     /locus_tag="PB780_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDE0733373.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            14430..15563
                     /locus_tag="PB780_001310"
     CDS             14430..15563
                     /locus_tag="PB780_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDE0733374.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            15563..16519
                     /locus_tag="PB780_001311"
     CDS             15563..16519
                     /locus_tag="PB780_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDE0733375.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            16618..17784
                     /gene="deoB"
                     /locus_tag="PB780_001312"
     CDS             16618..17784
                     /gene="deoB"
                     /locus_tag="PB780_001312"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDE0733376.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            17808..18626
                     /locus_tag="PB780_001313"
     CDS             17808..18626
                     /locus_tag="PB780_001313"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDE0733377.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            18643..19350
                     /gene="deoD"
                     /locus_tag="PB780_001314"
     CDS             18643..19350
                     /gene="deoD"
                     /locus_tag="PB780_001314"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDE0733378.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(19648..20334)
                     /gene="gpmA"
                     /locus_tag="PB780_001315"
     CDS             complement(19648..20334)
                     /gene="gpmA"
                     /locus_tag="PB780_001315"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDE0733379.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(20506..21087)
                     /locus_tag="PB780_001316"
     CDS             complement(20506..21087)
                     /locus_tag="PB780_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDE0733380.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(21220..21903)
                     /gene="rpiA"
                     /locus_tag="PB780_001317"
     CDS             complement(21220..21903)
                     /gene="rpiA"
                     /locus_tag="PB780_001317"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDE0733381.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            22222..22635
                     /gene="rpsL"
                     /locus_tag="PB780_001318"
     CDS             22222..22635
                     /gene="rpsL"
                     /locus_tag="PB780_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDE0733382.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            22719..23189
                     /gene="rpsG"
                     /locus_tag="PB780_001319"
     CDS             22719..23189
                     /gene="rpsG"
                     /locus_tag="PB780_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDE0733383.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            23283..25370
                     /gene="fusA"
                     /locus_tag="PB780_001320"
     CDS             23283..25370
                     /gene="fusA"
                     /locus_tag="PB780_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDE0733384.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            25542..26729
                     /gene="tuf"
                     /locus_tag="PB780_001321"
     CDS             25542..26729
                     /gene="tuf"
                     /locus_tag="PB780_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDE0733385.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
BASE COUNT         8434 a         4951 c         5684 g         7747 t
ORIGIN      
        1 ggtttatact atagccctgt tagtactcca gcagattttc ggatgcttac tcctattgta
       61 ctggagcaag cctttagaaa agctggaaca gaattgttag agccatatct tagttttaaa
      121 gtttatgcac cacaggaata tctttcacgg gcatataacg atgctcccaa atattgtgca
      181 aatatcgtaa atactcaact gaaaaataat gaggtcatta ttattggaga aattcctgct
      241 cgatgtattc aagattatcg caatgattta acttttttta caaatgggct tagtgtttgt
      301 ttagcagagc taaaaggata tcaggttacc actggcgaac ctgtttgcca gacccgtcgt
      361 ctaaatagtc ggatagataa agtaagatat atgttcaata aaataactta gtgcgtttta
      421 tgttgttata taaatatggt ttcttattaa ataagatgaa atattcttta atatagattt
      481 gaattaaagt ggaaaggagg agattgttat tataaactac aagtggatat tgtgtcctat
      541 ttgtggaaat aaaacaagac tacgaatacg agtggatact atacttaaaa atttcccttt
      601 atactgcccc aaatgtaaga acgaaacttt aattaatgtt caaaaaatga atataataac
      661 aatcaaagag ccagacgcca agacgcagag ccgataattt gagaaatgaa actctcatct
      721 tatcggctct ttttgtttat ctgaatttta ctgactagcc ttcaatattt cttgcttcat
      781 tgataccttt tgctagtgct tccattaagg atagttcttt gtctgtaaag ctatccatgt
      841 atttttctac ctgtaacctt cgagtgcttt taatcaaatt atctgtaggt aagaaaaatt
      901 catcaataga tacatggagt aatgatacaa gatcatacag cacttgtgta cttgggtgtt
      961 gtcctttgtt ttctatattt gttaaataac gtgggtcaat ttcaatcatt gttcccactt
     1021 gttcacgagt gagaccccgt ttcattcgag cctctttaat cgctaaacca acggctctaa
     1081 aatcgtattt atcttctttt ttacgcatac taaatcacct ctatacattt tactgttcct
     1141 atataaccat aaacaggtat agaaaaacat gttatatagt ttatgagttc ctatagaaca
     1201 ctgtggaatt tattaatgcg atgaatccaa tttcaaatag aaaggtcgct gattttattt
     1261 acctatacaa ctttataaaa tttagtcata ctcctagaaa gtagataaaa ttggcgactt
     1321 aatggaggga aaagtcgcca tgagaaagat aatctgtcat ctagttctct taattaagtt
     1381 cttatcaaaa aaacttatcc acctgtactg gatagatatg accatcaatg tggttttgca
     1441 gattacatag atagaaaaat caaagcatat gaacagagaa aacaatctgt ttatgtgctt
     1501 ttttggtcac ttaataaata tcttttagga tttctcttca tttatgcaac aaatcccccg
     1561 ttcgtgtcgg ggtttagtat gaagggagat aaagaagcac atgccactcc ctttcacgac
     1621 agaaaggggg tgaaaagcat gaaaccatct tcttttcaag aaaccataga aaatcagttt
     1681 gactatatct gcaaacaagt tattgaagat gaacgaaaag attacttcaa acatctagac
     1741 agactatcag caaaagaagt ctcgttttct gatatagata actaccttgt caactctttt
     1801 tctactgttg atacgtacgt cactgatttc caactattta aattatttgg attaagaatc
     1861 aatgttgaaa gtgatttatt aagtgaagcc ctgcgtcatc tttcagagaa aaaacggaac
     1921 attattttac ttcattactt tatggatatg agtgatgtgg agattgcgga gttactaaat
     1981 ttgaatcgtt cgactgttta tcgtcatcga ataagtggac tagccatgat taaagaattc
     2041 atgaaggagt gtaaagaatg aaaacagctt atccaagggt tcccttcccc ctcattgtaa
     2101 aggcgacaga tggcgacgtg gaagcaatta atcagattgt taaacattat cgagggtata
     2161 cctcaaaacg ttcattacgt cgcatgacag acgaatatgg aaattctcat atggttattg
     2221 atgaaaccct acgtggcaga atggaaacga aactcatctc taagatttta tcgttcgaaa
     2281 ttaaatagtc ccattttttc gtggaagcgt gtcgaatcgt atccacgctt cccgatttgg
     2341 gaggtttgtt attccatcaa agcatattgt gtgttcagta ggttttgata ggctaacaag
     2401 ccaaacgttc tttgacaacc aaataaaggc aatcgaatac gtttcggtaa gaaaagagct
     2461 gatgaactaa cacgccatga cctacaaaat tagtgagcga ttcatgttag taattcgaaa
     2521 gtaatcttta gcaggattgc tgccatgact ttcttatcgt gataatgata ctcccgtata
     2581 gccatagtcc gagcgttaga agcgtcacag gcaatgggta cggctacatg agaaccatgc
     2641 agaagtgaaa ttcctgtgag ctttgctaga gctgttcgat tgctaagata ttcttctctt
     2701 gtcagtgtat tattataaaa ctatttgata taattatagg tgaatattgg agttttccta
     2761 gcctataatt gggaaaggag taataatgat aacggtcaca aaaaaggatt tagttgagct
     2821 aggatatgga acttcttttt cagcagacat cataaaaaaa gcaaaagaat tgatgattac
     2881 aaaagggcat atctactatc aatctcgaaa gctagataga gtccctcgtg aagctgttga
     2941 agaaattcta ggaattcggt tttcaaatga aaacaggtct gagtagtttt tgcacagtaa
     3001 ggagtaaaaa ctatggttaa agacccaata aaaaaagcaa agaatggaac ctattatttt
     3061 cgtgcaaatc taggttatga ttctaaaggg aaaaaaattc aaaaatatcg tagtggattc
     3121 actactaaaa aagaagcaag agaagaatat tcgaaactgc tattgatgaa accagaagaa
     3181 ttaagtgaga ataaggataa aatgacgttt aaacattata tctttgaaat tttccttcca
     3241 tggtataaga cacaggtaaa gctcagaaca tatgaaaatc gcttaccaac tattaagaag
     3301 catttctctt attttgataa aatggctgtt tccgatattg agcctattga tgtgcaaaat
     3361 tggcaactaa aattatcaaa gaagtgtaag tcttcttatg taagagctgt tcaaggacta
     3421 ttttcagttg ctatggatcg agcaattgtc cttgggataa ccaccacaaa tccatctaag
     3481 attattggca atgtgaaaaa gcaaaagtct aaaattgaat tttggacaaa agaagaattt
     3541 gaaaaagtaa tctctctaat ttacaaagaa gattactatc aacacttttt atttatctct
     3601 ctgtggtttt tatttatgac tggaatgcga attggtgaag ctactgccat tcaatgggaa
     3661 gatattgatt ttgactcagg agtactaacc atcaacaaaa cactctatta caagaatcaa
     3721 tcgaattata gatttgttga accaaaaaca aaggcgagcg ttcgtcatat tgtattagat
     3781 aaatgcacat taacttattt atccgaatgg aaggaagtac aacaaaatct aattcagaca
     3841 gactttgtaa tgagctataa cggtattcct acccaaaaac atacactatc atatgctatt
     3901 gaacgctatg ctaagctggc tggtatccat cgaatcagaa tccatgggtt acggcattct
     3961 catgcttcct tgttaatcag tatgggagaa aatccactta tcattaaaga tcgtctagga
     4021 cacgaagata ttgagacgac tttaggaacg tatggacatc tttatcctaa cagtaatttc
     4081 gaggtagcta acaaattaaa tggagccatt tcctttaaag aagcaaaaca aaattatgat
     4141 acttcaccaa agaatcaatt tactgtagac tacttgcgaa acaaagaaat gaaaaaagtg
     4201 caataaaagt gcagtaaaaa gataagaaga gccagaaacc cttggtataa gaggatttct
     4261 agctctccta caactattcc cactccactg ttgctggtgg tttagaagtg atgtcataca
     4321 cgatacggtt gacgtgggct acttcgttta cgatacgtac agagattttt tgcaagactt
     4381 cccatgggat acgggcaaag tcagcggtca ttccgtcgat tgaagttaca gcacggatac
     4441 cgactgtgta gtcgtaagta cgtccgtcac ccataacacc gacagagcgg atccctggaa
     4501 gaacagtgaa atattgccag atatcacggt caagtccagc agctgcgatt tcttcacgca
     4561 agatagcatc agattcgcgt acgatttcta gcttgtcttc agtgatttcg ccaagaacac
     4621 gaataccaag accaggacca gggaatggct gacgccaaac gattgaatca ggcatgccta
     4681 actgagtccc taattcacgg acttcgtctt tgaataatgt gtttaatggt tcgatcaatt
     4741 caaattgcat atcttctggc agtccaccta cattatggtg agatttgatc gtctgtgctg
     4801 tttctgtacc agattcgata acgtcagtat acaacgttcc ttgtgctagg aaagagacgc
     4861 cttcttcacc agcaagtttt gttgcttcgt catcaaaaac atatacgaat tcattcccaa
     4921 tgattttacg tttttgttct gggtcagata cacctttcaa cttgtctaag aagcgatctc
     4981 ttgcatcgac tttgatgatg ttcaagccga attttccgcc tagactttcc attacttgtt
     5041 ccgcttcgcc tttgcgtagc agaccgtggt caacaaagat agatgtcaat tggtcaccga
     5101 tcgctttttg taaaagaacc ccaacgacac tagaatcaac gccacctgaa agaccaagca
     5161 agactttttt gtcaccaact tgttcacgga ttttttctac ttgcatatcg ataaagttat
     5221 ccattgtcca gttaccttca cagtgacaaa catcaaaggc aaagtggcgc agcaattcga
     5281 ttccgtattc ggaatgacgt acttctgcat ggaattggat accataaaaa ttacgtgttg
     5341 tgtcttggat agaagcaatc ggacaatctt tgcttgttcc gactgtttca aagccttcag
     5401 gaactttagt taccaagtca ccatggctca tccatactgt ttgttgtttt ggtgtttctt
     5461 taaacaagac agcttgatcg cttgttactt ctaacaaggc tttcccatat tcgcggtttt
     5521 tagcaggctc tacggaaccg ccaagtttgt acgtgatcaa ttgcatacca tagcaaatcc
     5581 ctaatacagg aatacctaaa ttgaagatct caggatcgat cccaaatgca ttctcatcat
     5641 atacactgtt tggtccgcca gaaagaatga ttccctttgg attcatcgct tttacttctt
     5701 ctgcagtaat gcggtggctc aataattcgg aaaagactcc aagatcacga atacgtcttg
     5761 tgatcaattg gttatactga ctaccgtagt ctaatacgat aatcttttca acgttcgtca
     5821 tatcggcaac gtttgtcaaa tttattaccc ctatcccttt aaattttatc tcaaaatagt
     5881 caaactattt taaaatgcta atattttcgc atgaaaatcg aatcgatgat atgattttca
     5941 gttttatgga gaattatatc ggctcttcct cgtgtaggca agatatattc ctgtaaattt
     6001 ttcaaattca catttttcca tacatctttt gccattttaa aggcttcttc acgatctccg
     6061 atagcaaaac gataataata attctctgga ttttggaaag ccgtatccag taacgcacca
     6121 aaacgatcca aataccattt ttcgatcagc acgggatcgg catcgacata gacagaaaaa
     6181 tcaaaaaagt cgctgacgta gatttgctga ttcgcaggta attgaagcgt attgatcccc
     6241 tcaacgatca agatatcagg ctgctggatc aattcgtact tgtctggaat gatatcatac
     6301 acatcatgtg agtaaacagg tacttttatc tcttcctgac cagattttac acggttcaag
     6361 aaatcgatca gcatctccat atcataactt tcaggaaatc ctttacggtc catgattcca
     6421 cgctctttta atatacgatt tggataaaga aatccgtcag tcgtgatcag ctgaacgttt
     6481 cttcgtttga aggtacgaga caaaatcgtc tgcagcagtc tggctgttgt actttttcca
     6541 acggctacac ttccggcgat cccgataata aaaggcggta cttctacgta tttatgaaga
     6601 aataaacctt tactcaagaa caaagattca aattctttca tataaagata aatcagatga
     6661 gcaagaggta catagatatc tcgaacatcc tgcatggaaa tgcggtcatt caaacttttg
     6721 atattatcta gttcatcttg tgtcaaaggt gcttgcccat tacgataaaa ttctcgccat
     6781 tcttctcttg ccacacggta gtagttcatt ggatcattca tattagcctc ctgcgttctc
     6841 ccatcaatgg aactatttta acacaaaata aacgcaaacc ccaccactta agagagattg
     6901 ccgatctttt tgtataatct ttataataga aagaaacgga gtcttattct tgacttgcgc
     6961 acggatacat aaaaggatag actgttgtca tgaaagaaat gagaaggatg gaagacatga
     7021 caaatcatta ctattctgaa cgcccagata cggcgcacga ttttgaacaa tgggcttttg
     7081 agttaaaagg cagaaccttt caatttgtaa cagattctgg tgtattttca cgtgaaacag
     7141 tggattatgg ttcacgtgtc ctgattgatg ctttcgattg ggaagagtta cctgatgaag
     7201 gaaagatctt agatgtggga tgcgggtatg gaccgattgg gttagctttg gcatttgcga
     7261 cacagcggtt tgttgaaatg gtcgacatta atgaacgagc ggtaagtcta gcgcaaggaa
     7321 acgcaaagcg taaccaaatc gaacatgttg atatccatca atcgaatatc tatgaagcag
     7381 ttcatgaaga aacctatgca gctatcgtaa gcaatccgcc gatccgtgct ggaaaaaaag
     7441 ttgtacatga aattttgaca ggcgcatatc ctcggttgaa gaagggcggt acactgacaa
     7501 ttgtgatcca gaagaaacaa ggagcgccga gtgcgcaaaa gaaaatggaa gaaacatttg
     7561 gtaatgcaga aatcgtcaca aaagataaag gatattatat catcaaaagt gtgaaagaat
     7621 aactagctga aaaatagtgg agcgacgaaa gagtggaact gggcgtgcat gtacgggtac
     7681 gtatcagttc tttttttgtt gagaagtttt taaaaacaaa gaactatttt ttcttcaaaa
     7741 agaaatagta ggaacagagt cgttgttttt tacgataaaa taaataagat acagaacttg
     7801 tatagaaggc tgccaagtcg gtagataaag gaggttttta tcaaaaaaca tactttcctt
     7861 ttttctctat tcttttcttg ggggttttgg tacactgata gggaatttag atagaaggga
     7921 gccaatagga tgaagctgac catcagagat atcgcagaaa tggcaggcgt atcagtaaca
     7981 actgtctcac agattttgaa taacaaagga agcagattta gtgataagac aagaaaaaaa
     8041 gtgttgtcga tcgtgaatga acatcattat aagcccgatt atttcgcttc aaatgtgatc
     8101 aatcgacatt caaagaccat cggaatgatc gtccctgatg tcactgattt cttcttctcc
     8161 aaactgatcg aaggtgtaga gagctatttg aatcctttgg gctatgtgat catgttatgc
     8221 aattctcgtc atagccaaga aaatgagatc aagtatttac aagagctttc ccaccgctca
     8281 gtggacggaa tcttgctggc cacaccaaat attttacctg agaaatatgc attagacagc
     8341 ggtttttatc aaaaaatgcc aatcatttta atcgatcgcg gattgaatcg tcgggataac
     8401 ggacggctta tcgtaaagga atatgagggc gcctatcaag ctgtttcgct gtttattgaa
     8461 aatggccata cgaagatcgg catgctgaag gaaaccactg gatactatca attagaagaa
     8521 cgtttcaacg gatatcgtca tgcgctgaaa gatcatggcc tgccttttaa tgggaaattt
     8581 gttgaacatg gagagttgac tgtccaaggt ggatatactg catcaaaaaa attactcaaa
     8641 cataaagata tcacggctat tttttgcggg aatgatgcca tggcgatcgg atgctatcag
     8701 gcgatcgatg aattgggcaa aaaaatccca gaagatatct cagtcatagg attcgatgga
     8761 ttaaagctgt cagaatacat gattccgcaa ttgacgacag tgcagcagcc aagttttgat
     8821 attggatttt atgctgcacg tttcctgatc gatacgatcg aatttcctca aagaaaagtt
     8881 ccgaacaaag tgttcgaaac aaaattaatt attcgtgaga gcgtaaaagt attgacaaag
     8941 ggctaactcg gcggtatatt gcttatgtaa gcatttacaa aggctagtta gtgctttttt
     9001 tatttttagt aaaccagttt actaaagtgg tttacctttc ctaaaaataa tgtataatac
     9061 cgaatgaaga caaggaggat caggcaatgc gaatggtgga tctgattgaa aaaaagaggg
     9121 atggaaacga gctgtcgaaa gaggaaatcg agtacatcgt cacgaactat acgaatggaa
     9181 agattccaga ttatcaagtc agtgcattat tgatggcaat tttttatcaa gacatgacaa
     9241 atgaagaaat cacgaactta acattggcga ttgctaattc tggtgatgtc atcgatttga
     9301 gttctcttga aggaataaag gtagataagc attcgacagg tggcgttggt gacacgacca
     9361 cattgatttt agccccccta gtagctagtg tcggagtgac agtggctaaa atgtctggac
     9421 gcggtctagg ttacacaggt ggtacactag acaagctaga agcaattccc ggatttcaga
     9481 tcgagctttc ggatgaagaa tttgtacgca ttgttaatga aagtaaagta gcggtaattg
     9541 gtcaatcagg aaacttggca ccggcggaca aaaaattgta tgccttgcgt gacgtcacag
     9601 cgaccgttga ttctcttccg ctgattgcca gttcgattat gagtaaaaaa atcgcggcag
     9661 gagcagatgc aatcgttttg gatgtgacca ctggagacgg tgctttcatg aagaacatcg
     9721 aagatgcaag acgtctggca aagaccatga cgagcattgg gaaactggct aaccgagaaa
     9781 cagtggcggt tatctcagat atgtcagaac ctcttggcga agctatcgga aatagtctag
     9841 aagttgtcga ggcaatcgaa acattacaag gaaatggtcc agaagatcta gtagaaatgt
     9901 gctacgcctt aggaagccaa atggttgttc ttgctggcaa agctaagaca accgatgaag
     9961 caagaacgtt gcttcaagaa gctctggaat caggtaaagc gttagcaaaa ttcaaagaaa
    10021 tgatccaaaa tcaaggggga gacccagcga tcgtggaaca tccagaacga attttgactg
    10081 cacgctacac aatggagctt cctgctaaac aaagcggagt tgtgtcaaaa atcgtggcaa
    10141 atgaattagg aattgctgca atgatgctag gtgctgggcg taaaaccaaa gaagaagata
    10201 ttgatcacgc tgttggattg aaactgcata aaaaaattgg tgacactgtg acaaaaggtg
    10261 agtcactatt gacgatctat agtaacgacg aagaaatcac ttctgtcatt gaattattat
    10321 ataaaaatat tgaaattggt gaatcggcaa tgaagccaac gttgattcat gacatcatca
    10381 cagaataaag gaaaaaagcg gactttaccg ttttaaagaa aaggggaaaa gaaatggaac
    10441 taaaccgaat gatcgaccat actattttga aagcagacgc atctaaagaa gctgtgatgc
    10501 aaattatcga agaagcaaaa aaataccatt tttattctgt ctgcatcaat ccagcatggg
    10561 ttgcgctagc gaaagaacaa ttacaaggtg agccagtagc cgtatgtaca gtaatcgggt
    10621 ttcctctagg agcaaacaca cccgagacaa aagcatttga aacaacagat gccatcaata
    10681 acggtgcaga tgaagtcgat atggtcatca atatcggtgc tttgaaatca aaggatgatc
    10741 aacaagtcca aaaagatatc gaagcagttg ttgaagcagc aaaagatcgt gcgcttgtca
    10801 aagttattat tgaaacatcg ttattagata gagaagaaat catccgagct tgcgaaatag
    10861 caaaagctgc aggcgctgat tttgtcaaaa catcaactgg tttctcaaca ggcggagcaa
    10921 aggtagagga tgtccgtttg atgcgtgaaa ctgtaggacc agaaatgggt gtcaaagctt
    10981 ccggcggtat ccataacgaa gaagaagcaa tggcaatgat cgaagctggt gctacaagaa
    11041 tcggaacaag cgcaggagta gcaatcgtat ctggttcaac tggtgaaggc tattaatttt
    11101 ttcaaaggag aagctcatgg cagaactcaa acaagaatgg atcaacacag ctattgaagc
    11161 gttagataaa gcctatgttc cttattccca ttttccggta ggcgcttgtc tcgtaacgga
    11221 atcaggcaag atttatcaag gcatcaacat tgagaatgca tcatttggat taacgaattg
    11281 tgccgagcgc acagcttttt tcaaagcagt atctgaagga gaaaaaacat ttacgcatct
    11341 agtcgtagcg gggcacaccc ccgatcctat ctctccttgt ggtgcatgtc gacaagtaat
    11401 ggcagaattc tgttcccctg atatgccggt tacgctagta ggagacaatg gtgtgacgaa
    11461 ggcaacgacc gttcgtgcac tgctgcctta tgcatttact gaaaaagatc tataactcat
    11521 gttcattcag tcagctgact gttgaaaaat aaaacaatta ttttaggagg ctttacagaa
    11581 atgaaaaaag caaaattatt tggtttaggt gctgtcgcac tagcagcagg gttattttta
    11641 ggtgcatgcg gcaacaacgg ctctactgat tcatccggtg gcaaaagctc atcagacaca
    11701 acaactgcag cattgatcac agacacaggt ggtgtagacg accgttcatt caaccagtca
    11761 gcttgggaag gactagaaaa atgggggaaa gatcaaggac tttcacgagg aaatgatggc
    11821 ttccaatatt tccaatcttc aaatgaatca gattacatcc caaatattga ccaagcattg
    11881 aatgctggat tcaaaacgat ttttggtatt ggttataaac taaaaccagc gatccaagaa
    11941 caagcaacaa acaatacagg tacgaacttt gttattatcg atgatgtcat cgatggatta
    12001 gataacgttg tatctgcaac tttcaaagac aacgaagctt cttatcttgc aggtgttgca
    12061 gcagcttata cgactgaaac aaacgtagtc ggctttatcg gcggtgtaaa aggtgaagta
    12121 atcgatcgtt ttgatgctgg attcaaagcc ggtgttgatg caggtgcgaa agaattagga
    12181 aaagaaatca aagtattgaa tcaatatgct ggtgacttct ctgctccaga taaaggacgt
    12241 tcaattgctc aaggtatgta tgcacaaaac gcagatatca tcttccatgc ttcaggcggt
    12301 acaggtaacg gtgtattcca agaagcaaaa tcattgaacg aatcaggcga caaaaaagtt
    12361 tgggttatcg gtgttgaccg cgaccaatca gacgaaggcg aatacacatt aaacggcgaa
    12421 aagaaaaact tcacattgac atctacactg aaagcagttg gaacagtagt agaagatttg
    12481 gcacaaaaat cagcggacgg taaattccct ggcggcgaac acactgttta tggattgaaa
    12541 gaagacggtg ttggtctaac agaaggacaa ctttctgacg aagcgaaaaa agcagttgat
    12601 gaagcaaaag aaaaaatcat ttcaggtgac gtaaaagttc cagaaactcc tgaagaaaac
    12661 taaaaccatt tctatagcct tgttagacac atgtttgaca aggctatggt ttgattagaa
    12721 caagaattgt tcatttatcc aagctgcctt gtgacagttt ggataaggga acaactccct
    12781 gaagtaatgt ttgtttcaaa agaagaacgc tcactaagcg tgaatcttag tcaatcgttg
    12841 ccctagatag aaatcgttag gatgtgaaaa aaaggtggtt caagaaaata ttgtcataga
    12901 aatgcagaat attaccaaag aatttggaac attcaaggca aatgacaaca tcaatttaca
    12961 agtaaaggca ggagaaattc atgcgttgct tggagaaaac ggtgctggca aatctacatt
    13021 gatgaacgtg ctttccggat tattagagcc gacatcaggg aaaattttga tgcgtgggaa
    13081 agaagtaaag atcacaagtc cgaccaaagc caaccaatta gggattggga tggtccatca
    13141 gcactttatg cttgttgatg cctttactgt aacagaaaac atcgtgttgg gaagcgagcc
    13201 tagtcgcgca gggatgcttg accataaaaa agcacgaaaa gagatccaaa aagtttctga
    13261 acagtatgga ttgtcagtca acccagatgc ttacgttcgt gatatttcag ttggaatgga
    13321 acaacgggta gaaattttaa aaacacttta ccgaggagca gatgtactga tttttgatga
    13381 gccgacagct gtattgaccc ctcaagaaat tgatgaatta atcgtgatca tgaaggaatt
    13441 agtcaaagaa ggcaagtcga tcattttgat tacgcataag ttagatgaaa tcaaagcagt
    13501 agctgaccgc tgtacagtta tccgccgtgg aaaaggaatc ggtacagtca acgttaaaga
    13561 cgtaacctca caacaattag ccgatatgat ggttggaaga acggtttcat tcaaaacgat
    13621 gaaaaaagaa gcgaagcctc aagaagtcgt tttgtctatt gaaaatctag tggtgaaaga
    13681 aaatcgtgga ttagaagccg tgaaaaactt gaacttagag gttcgtgctg gcgaagtact
    13741 tggtatcgct ggaatcgatg gaaacgggca gtcggagttg atccaagctt tgactggttt
    13801 gcgaaaggca gaaagcggac atatcaagct aaaaggggaa gacatcacta ataaaaaacc
    13861 ccgaaaaatc actgaacatg gtgtaggaca cgtgccagaa gaccgtcata aatacgggtt
    13921 agtcctagat atgacattat ctgaaaatat cgcactacaa acctatcatc aaaaacctta
    13981 cagtaaaaac ggcatgctga attattcagt aatgaatgaa catgccagag aattgatcga
    14041 agaatatgat gttcgaacaa cgaatgaact tgttcccgca aaagctttat caggcggaaa
    14101 tcagcaaaaa gcaattatcg ctcgtgaagt cgaccgagat cctgatctgt tgattgttgc
    14161 aaatccaact cgtggactgg atgtaggtgc gatcgaattt attcataaac gtctgatcga
    14221 acaaagggac aaatacaaag cagtgttact gatcagtttc gaattagaag aaattttaaa
    14281 tgtttcggat cgtattgctg ttatccatga aggagaaatt gtcggtatcg ttgatccgaa
    14341 agaaacatct gaaaatgaat taggtctatt gatggcgggc tataccttag aagaagcacg
    14401 aaaagagtta gaaaaggggg ctggcgcaca tgaatgatcg cagcgaaaaa ttccgtaatt
    14461 tattagtgcc tgtactttca gttgttttag gattgatctt aggcgcgata ttgatggctg
    14521 cttttggttt tcagcccatc caaggttatt cttctatgct agatgcagcc cttggttcgc
    14581 aaagagggat aggtgaaacg ttaaggcaag caacgccgtt gatttttaca gcattaggtt
    14641 tttctgtagc taactcagct gggtttttca atattgggct ttctggacaa gcactttgcg
    14701 ggtggatcgt cagtgtctgg gtggcacttg cgtttccaga tctgccaaaa gttgttttgc
    14761 ttccgttatg cgtgatctta ggtgccttag ctggtgcgat ggcagcggct gttccaggtt
    14821 tacttcgagc attttttgga acaagtgaag tgatcgtaac aatcatgatg aactatatct
    14881 tattatacgt aagcacattt gctttacatg aagtgatgcc tgaaagctat cgctcaagtt
    14941 tggactcttc caatatgatc agtcaaaatg cctcactgcg gttaccatgg ttaacacaag
    15001 cttttggcgg ctctcgcgta aactcaggat tatttttagc aatcatcgcg cttattttcg
    15061 tttggctgct gatgaagaaa acgactttag gatttgaaat ccgttctgtt ggattgaatc
    15121 cttttgcttc tgagtatgca ggaatgagta gtaaacgtac gatcgttttg tcgatggtca
    15181 tttctggcgc tttggcaggg cttggcggtg tggttgaagg attaggaacg tatcaaaact
    15241 tctttgttca aacgacttcg ctatctattg gatttgacgg aatggctgtt tccttgcttg
    15301 gttctggatc agcaatcggt attcttttat cagcgatctt gttcagtatt ttgaagatcg
    15361 gtggacttgg catgcagaca ggagcaggtg ttccgtttga gatcgtcaat gtatcgattg
    15421 ctttgattat cttctttgtc gggatcaact tcctgattcg tttcgtcatg gctaaagtct
    15481 tccaagggaa aaaacaagaa gagatcgttg cgacaataga atcgaaacca acgaatcaaa
    15541 accatgaagg gggcgaacta taatgtcgac tatcgaatta attgccttga tccttacttc
    15601 tacactggtt tattcaacac cgctaattct aacgtcacta ggcggaactt tttctgaacg
    15661 aagcgggatc gtaaacgtag ggctagaggg aatcatggtc atgggtgctt ttagttccat
    15721 cgttttcaac ttgacgatga gtccatcact tggttctatg acaccatgga tcgctatttt
    15781 agtgggtggc gtcgtaggta tcctattttc tctgattcac gcagtagcaa cagtcagttt
    15841 gcgtgctgac catattatca gcgggacagt aattaatctg atggcacctg ctttaggtgt
    15901 tttcttgatc aaagcatggt atggtaaagg tcaaactgat aacatcacgc agaatctggg
    15961 gtatttttct ttcccaggat tagcagatat tccggtcatt ggaccaattt tctttaaaaa
    16021 tacattctta cctgcttatt gtgctatttt gattgctgtc ctagcttggg tgatcatctt
    16081 caagacgaaa ttcggcttgc gcctccgttc agttggtgaa aatcctcaag ctgccgacac
    16141 attaggtatc aatgtatatg ctatgcgtta ttcaggcgta ctgatttctg gcttgttagg
    16201 cggtatgggc ggtgctgttt ttgctcagtc gatttccggt aacttttcag ctggtacgat
    16261 cgttggacaa gggttcatct ccatggctgc tatgatcttt ggtaagtgga atcctttagg
    16321 ggcaatgggt gcctcattgt tctttggttt tgctcaaagc ttgagtatca ttggcgcaca
    16381 attaccagtt atttcttcga ttccgccagt attgctgcaa attgctccat atttgctgac
    16441 aatcattgtg ctggttgttt tcttagggaa agcttctggt ccaaaagcaa acgggaagaa
    16501 ctatatcaaa tcaaaataat cttttagcga ataaaaaggg tgggttacgg gaagtgtttt
    16561 tcgtctcgcc ctttttgttt taaaggcaat aaaaataaaa agaaagaagg aataatcatg
    16621 tttaaacgcg tacatctgat cgtcatggat tcagtaggaa ttggagaagc accagatgcc
    16681 gataagttcg gtgatgttgg ttctgataca ttaggacaca ttgcgaaaga agcaggattg
    16741 aacgttccac atttagagca gcttggttta ggaacgatcc gtcctcttga aggtgtggaa
    16801 tcagtgaaag atcatcaagg ctatgcgacg aaattagaag aagtttctgt agggaaagat
    16861 acgatgacag gccactggga aatcatggga ttgaatatca aaactccatt tcgtgtgttt
    16921 cctaatggat tccctgatga tctgatcaaa caaatcgaag aattttctgg ccgtaaagtc
    16981 gtatgtaaca aaccttatag cggaacagct gttattgacg attacggtga acaccaaatg
    17041 aaaacaggcg atttaattgt atatacttct gccgatcctg tccttcagat cgcggcacat
    17101 gaagaaatca ttcctttaga agaattatat cggatttgcc aattcgttcg tgatataaca
    17161 aaagatgatc cgtatatgat tggccgaatc attgcacgtc cgtacgtggg tgaaccagga
    17221 gacttcacac gaacaagcaa tcgccatgat tatgcgttga atccattcgg acacacagtc
    17281 cttgattctt tgaaagaagc aggaaaggac gtgattgctg ttggaaagat caacgatatt
    17341 ttcaacggac aaggaatcac tgaatctgtc cgcacgaaaa gcaatatgga tggcgttgac
    17401 cagctgctga aagttatgaa aaacgatttt tcaggaatca gctttaccaa tttagtagat
    17461 tttgatgcat tatacggtca tcgtcgggat gtagtcggct atgcgcatgc gattgaagaa
    17521 tttgacttac gaattccaga attgcttgaa gcgatggaag aagacgactt gctaatgatt
    17581 acagccgatc acggaaacga tccgacgttt actggtaccg atcatacgcg agaatatgta
    17641 ccgttattgg tttacagtaa aaaaatgaaa gaacaagggg cattgcctca aggatattat
    17701 tcagatattt cagcaacgat tgctgagaac tttagtgttt cttcgactga aaatggcgaa
    17761 agcttcttga aattattaaa ataatctttt taaataggag aaagcaaatg accaagttaa
    17821 gcgagatgtt acaagaaacg acccagttta tccttgataa aggagtaaaa gaaattgatt
    17881 ttggtttgat tctaggttca ggattaggtg aattagcaga tgaaatcgaa gatgctgtaa
    17941 tgatttctta tgatgagatc ccgttctttc ctgtttcgac agtagttggg catgcaggaa
    18001 agctagttta tgggtctctg gcaggcaaga aagtgttagc catgcaaggg agattccact
    18061 actatgaagg tcattccatg caaacagtga cctatccagt acgtgtgatg gccggactag
    18121 gggcccattc agtcattgtg actaatgcat gtggtggtgt caacgaatca tttgtaccag
    18181 gagatttgat gctgattacc gatcatatca attttactgg tgaaaatcct ttgatcggtc
    18241 cgaatgaaga tgcaatgggc cctagatttc cagatatgag tcaagcttat acgcctgact
    18301 atcaagcaac agcaaaagca gcagcagaaa aactaggttt ggatcttaaa gaaggcgtat
    18361 atatgggcta ttctggacca acttatgaaa cgcctgcgga gatccgaatg tctcggacaa
    18421 taggagccga tgcagtaggg atgtcgactg ttccagaagt catcgtagca gctcacagcg
    18481 gattaaaagt gttaggaatc tcttgtatta ccaacttagc agcaggtatg caagcgaatc
    18541 tgagccatga agaagttgta gaaacaactc agagagtcaa acaatctttt aaagcattgg
    18601 ttaaagaaac attgacatta ttgtaatagg aggaacaata aaatgagtgt gcatattgaa
    18661 gcgaaagaag gcgagatcgc cgataagatt ttacttccgg gagatccttt gcgagctaaa
    18721 tatattgcgg agactttttt ggaagatcca gtttgttaca atcaggtacg cggtatgtta
    18781 ggctttaccg gcaaatataa aggagaacgt atttctgttc aaggaacagg aatgggaatg
    18841 ccatcagcca ccatctatgc ccatgaattg atccagtcgt atggtgtaaa gaaattgatt
    18901 cgagtaggta catgcggtgc attgtcaaaa gacgtccatg ttcgtgattt agtactagca
    18961 caaggtgcag cgaccagttc ttcgatgatc gaaaaaaatt tccaagcctt tcattttcct
    19021 ccaatcagtg atttcaatct attactgaaa gcatatgaga tagctaaaga aaagggctat
    19081 acggttcatg taggaaatgt tctttcggaa gattctttct acaaagatga tttgacagag
    19141 acgttccaat tagctgaact tggcgtttta ggagtggaaa tggaagcagc ggctctttat
    19201 tacttaggcg cgaaatacca tgtgcagaca ttaagtttga tgaccgttag cgaccatttg
    19261 atcaccggtg aagaaacaac agctgcagaa cgtcaatcta cgttcaatga aatgatcgaa
    19321 gtaggattgg aaacagcgat agcagaataa gaaggaaatg aaaaagctgc cctgcattaa
    19381 gtaataagaa catccaagca aaaatagctc ctcatgtttt tgacttggct ggacttatta
    19441 ttgcaaatgt aagcttttga acaccgttta ccaagaggtt gtgaatctgc cgtttaactc
    19501 tgagtcacaa ggaacaattt caaaaaacag cttttcatgt ttgatgaata ttgtgacttg
    19561 tgaccgagaa gttggcagga gaaaaccgtt tatacgaaaa aagggactgt gacaaatcgt
    19621 gtcacagtcc cttttttata gtgtttttta taggtagtat tttttagcaa ctgttaagtc
    19681 atcatttaat tcataaacaa gtggttgacc tgttggaatt tcaagatcca tgatgtcttc
    19741 gtcagagata ccttcgatat gtttagctag ggcgcgtaat gagttaccat gtgcagctac
    19801 taaaacagtt ttgttgtcta gtaatgctgg agcaatttca tcttgccaga atggcaaagc
    19861 gcgttctaat gtcactttca agttttctcc accaggaata tcgcgtttgt ctaacattgc
    19921 gtaacgacgg tcatttgctg cagaaccttc gtcagtttct tccattaatg gaggaagagt
    19981 atcgtaagaa cgacgccaga tgtgcacttg ttcgtcgccg tatttttcag ctgtttcttt
    20041 tttgttcaaa ccttgtaatt taccatagtg acgttcgttt aagcgccatg atttgatttg
    20101 tggtacccaa agttgatctg aatattctaa gatcaagtta catgttttga ttgcacgagt
    20161 taaaacagaa gtgtaagcaa catcaaattc gattccagct tctttgattt tgcgtccgcc
    20221 ttcttttgct tcttcgattc cttctggtgc tagatttaca tcagcccaac cagtaaattg
    20281 gttcaatgca ttccattcac taagaccgtg acgagaaaaa actaattttg gcatgtttag
    20341 ctctcctttt aagataggtt tatatttctg aaattttcag ttctgtttta ttgtacaatt
    20401 ttttggaaga gatttccatc gaaaaattag ataagattct atgagaaaac aagtagagaa
    20461 tataaaaaaa cgaacagtgc ccacgcaact gttcgttttt aggtattaga tgcataattg
    20521 ctcgatttct ttttcaggta aattgatata tttataacat gttccaactg aaacaccgca
    20581 ttttgaagaa ataaattgga tcgtttcttt tttttcataa tagagacgtc ggattttttt
    20641 gatcgttttt gcatcgattt ttggtcttcc gccgattttt ccttttttac gagcttcatc
    20701 taatccaact aatgtacgtt ctttgatcaa tgcgcactcc atgtttgcta ggttttccat
    20761 taattggaaa tagatttttc ccatttcgcg cgtatcgatt ccttcatcaa gactaatcaa
    20821 gtgcagcccg cgatctttga ataattgcgt gagttctgtt aattgccgcg tagatttgcc
    20881 taatcgatga agttggcaaa tgactagtgc atcgccttgt tgcatgtcag cgatcactga
    20941 ctccaaatga acagcagtag aatcattttg ttccacttca tatgtctcat ggaaaatttc
    21001 ttgacaacca aagtcagata attgatcaac ctgagttgtt aaatcaacgt catttatggt
    21061 tgtgcgtgca taaccaataa ttttcatggt gattcctttc tagcagtgat tttctaactg
    21121 gatataacag taaaaatgta gcataaagta cgtcaaaaag atagtttttt cgcaaatttt
    21181 ccgctgtctt tttttattgg atgatcaagc agtttttctt tatttacgac ggattgcgtc
    21241 aagaatctca acgccttttt ctgagccgac aactacttta gaaaccatgt ttaggaaaag
    21301 cccatgttct actaccccaa caagttcatc taaataggta gctaagcttt ctggctgttc
    21361 gatgacttct agatgcagat caatgatgta atgaccacca tctgtggtta agacttctcc
    21421 gttttcgtct gtacgcaaga cgggttggaa tcttttttct tcaaaaatat gaagtagctg
    21481 ttgggagcca taagggatca cttcaactgg gagtgggaac ttcccaagtt tatcgactaa
    21541 tttagaagaa tcaacgatcc aaatcgtttc ttttgagtac gtagcgacta ttttctcgaa
    21601 aagcaaagca gcgccgccgc ctttgatccc ttgaaaatca gcactgattt catcagcacc
    21661 gtctattgtc aaatcgacta cggggacttc atcaatactc tttaacggta ttcctaattt
    21721 ttccgcttgt tcttttgtcg catttgaagt agtaactcct gtgatacgta aaccttcttc
    21781 acgcatacgg cgaccaatct cttctaccat ataataggcg gttgaaccgg tcccgagacc
    21841 tacgatcatc ccgtccttta cgaatttcgc ggcttcaatg ccagctaatt gttttaaatt
    21901 catctttgcg tccacctttt tttagtttga gcatcgtgct ttcctcacta ttttagcgtg
    21961 atttacggta aaaagatacc attctttttt attccaataa agctttttca aaaaaaattg
    22021 catctcttgt tgacatgttt tttcattcgt gatattctaa caaaggtgct ttatctacag
    22081 gtttctgtga agaaacgtgc tgactgttta tcaaagcata tttgataagg atgcagacat
    22141 tgcatttatt ttttatcaca aaaaaagaac cacctggatg tgtggaacta gacgcaaatc
    22201 gtgggaagga ggaaacaagg aatgcctaca attaaccaat tagtacgtaa acctcgtaaa
    22261 tcaaaggtgg aaaaatctaa ttcacctgct ttgaacaaag gatataacag ctttaagaaa
    22321 actcaaacta acgtaaactc acctcaaaaa cgtggggtag caactcgtgt gggtacaatg
    22381 acacctaaaa aaccgaactc agctttacgt aaatatgcac gtgttcgttt gtcaaactta
    22441 attgaagtta ctgcttatat cccagggatt ggtcataact tacaagaaca cagtgttgta
    22501 ttattacgcg gtggtcgtgt aaaagactta ccaggggtac gttaccatat cgttcgcggt
    22561 gctttagata ctgctggtgt taacgaccgt aaacaaagcc gttctaaata cggaactaaa
    22621 aaacctaaag cttaatttta attattaaac aaatgaaact tgcttctact atatagaagt
    22681 aattactatg aacaatattt cggaaggagg agttacggat gccacgtaaa ggtcctgttg
    22741 caaaacgtga tgttttacca gatcctattt ataactcaaa attagtaact cgcttgatca
    22801 accgtgtaat ggttgacgga aaacgcggga ttgcagctaa cattatctat aatgcctttg
    22861 acttgatcaa agaatctaca ggtaacgatc cattagaagt gtttgaacaa gcaatgaaaa
    22921 acgttcaacc tgttcttgaa gttaaagcac gtcgtgttgg gggttcaaac taccaagtac
    22981 cagttgaagt tcgccctgaa cgccgcgtta ctctaggtct tcgttgggta gtaaactacg
    23041 ctcgcttgcg cggtgaacac acaatggaag aacgcttagc aaaagaaatc atggatgctg
    23101 caaacaacac aggtgcttct gttaaaaaac gtgaagacac tcataaaatg gctgatgcca
    23161 accgtgcgtt tgctcactat cgctggtaag attctctctg tttggtcggc ttttataagt
    23221 cgacctcaga tagtgtcctt atttttagca actagatagt aaagagagga gaaacaagga
    23281 gaatgaccag agaattttct ttacaaaaca ctcgtaatat cggtatcatg gcccacatcg
    23341 atgctggtaa aacgactgct actgaacgta tcttgtacta tactggtaaa atccataaaa
    23401 ttggtgaaac tcatgaagga gcttcacaaa tggactggat ggaacaagaa caagaacgtg
    23461 gtattacgat cacttctgct gctactacag cacaatggaa gggtcaccga atcaacatca
    23521 tcgacacacc aggacacgta gacttcacag ttgaagttga acgttcattg cgtgtacttg
    23581 atggtgctgt aactgttctt gatgcacaat caggtgttga acctcaaact gaaacagttt
    23641 ggcgtcaagc aacaacttat ggtgtacctc gtatcgtatt tgcaaacaaa atggacaaga
    23701 tcggtgctga cttcttatat tcagtatcaa ctttacatga ccgtttgcaa gcaaacgctc
    23761 acccaatcca attaccaatt ggtgcggaag acgacttcac aggaattatc gaccttgtaa
    23821 caatgaaggc tgaaatgtat acaaacgatc ttggaacaga gatcgaagaa acagaaattc
    23881 ctgaagaata ccgcgaatta gctgaagaat ggcgcgaaaa attggttgaa gctgtagctg
    23941 aaactgatga agaacttact ttgaaatact tagaaggtga agaaatcact gaagctgagt
    24001 taaaagaagg tatccgccgc gcaacagtta acgtagaatt ctacccagta ctatgtggtt
    24061 cagcatttaa gaacaaaggg gttcaattac tattggacgc tgttcttgat tacttgccat
    24121 caccgttaga tatcccagct atcaaaggga ttgaccctaa aactgatgaa gaagtagaac
    24181 gtcctgcaga cgacagcgct cctttctcag ctttagcatt taaagttatg actgacccat
    24241 tcgtaggtcg tttaactttc ttccgtgtgt actctggtgt tttacaatca ggttcatacg
    24301 tacaaaacgc aacaaaaggc aaacgtgaac gtgttggtcg tatcctacag atgcatgcca
    24361 actctcgttc agaaatctct gaagtttacg ctggagatat cgctgctgct gttggtttga
    24421 aagacacaac tactggggac acactatgtg atgaaaagag tctagttatt cttgaatcca
    24481 tggaattccc agaaccagtt atccaagtcg ctattgaacc taaatcaaaa gctgaccaag
    24541 ataaaatggg tgtggcttta caaaaactat ctgaagaaga tcctactttc cgtgctgaaa
    24601 caaacgtgga aacaggcgaa acaatcatcg ctggtatggg tgaacttcac ttagatatca
    24661 tcgttgaccg tatgcgtcgt gaattcaaag ttgaagctaa cgtaggtgcg cctcaagtat
    24721 cttaccgtga aactttccgt gctggcactc aagccgaagg taagtttgta cgtcagtctg
    24781 gtggtaaagg tcaatacggt cacgtatgga ttgaatttac accaaatgaa gaaggtgctg
    24841 gtttcgaatt tgaaaatgcg atcgtcggtg gggttgttcc tcgtgaatac attccagctg
    24901 ttgaaactgg tttgaaagat gctatggaaa atggtgtctt agctggctat ccattagtag
    24961 atatcaaagc taaactttac gatggttcat accatgatgt cgattcaaat gaaactgcct
    25021 tccgtgttgc tgcttctatg gcattacgtg cagcagctaa gaaagctaat ccagtaatcc
    25081 ttgaaccaat catggctgta gaagttgtta tccctgaaga ttatttaggt gatgttatgg
    25141 gtcacgtaac tgctcgtcgt ggacgtgttg aaggtatgga agctcgcgct ggcggccaac
    25201 aagttgttcg tgcgatggtt ccacttgctg aaatgtttgg ttatgctaca actcttcgtt
    25261 ctgctactca aggtcgtggt acatttacaa tgacatttga ccactacgaa gatgtaccaa
    25321 aatcagtaca agaagaaatc attaagaaaa acggcggaaa agctgaataa tttcttaaaa
    25381 ccggatagtc ctgcaaagag attatccaaa gagactatat ttttaatata gtttcgtgta
    25441 aaataattat cgatgatatg ggcatgtggt gtaccttgtg tacatcatat acctatcaga
    25501 caaataaaaa atatattgta tttttaggag gacattttaa aatggcaaaa gaaaaatttg
    25561 accgttctaa accacacgtt aacattggta ctatcggaca cgttgaccat ggtaaaacta
    25621 cattaacagc tgcaatcaca actgtactat ctaagaaaaa cggcggccaa gctatggctt
    25681 acgatcaaat cgatggtgct ccagaagaac gcgaacgcgg aatcacaatc tcaacagctc
    25741 acgttgagta tgaaacagat acacgccact acgctcacgt tgactgccca ggacacgcgg
    25801 attacgttaa aaacatgatc actggtgctg ctcaaatgga cggagctatc ttggtagttt
    25861 ctgctgctga cggcccaatg cctcaaactc gtgaacacat cctattgtct cgtcaagttg
    25921 gtgttcctta catcgttgta ttcttgaaca aagtagacat ggttgatgac gaagaattac
    25981 tagaattagt tgaaatggaa gttcgtgacc tattaacaga atacgaattc cctggtgacg
    26041 atgttcctgt agttgctgga tcagctttga aagctctaga aggcgacgct tcatacgaag
    26101 aaaaaattct tgaattaatg gctgcagttg acgaatacat cccaactcca gaacgtgaca
    26161 acgacaaacc attcatgatg ccagttgaag acgtgttctc aattactgga cgtggtactg
    26221 ttgctacagg tcgtgttgaa cgtggacaag ttcgcgttgg tgacgaagtt gaagttgttg
    26281 gtattgctga agaaacttca aaaacaacag ttactggtgt tgaaatgttc cgtaaattgt
    26341 tagactacgc tgaagctgga gacaacattg gtgctttact acgtggtgtt gcacgtgaag
    26401 acatccaacg tggacaagtt ttagctaaac caggtacaat cacacctcat acaaaattct
    26461 ctgcagaagt atacgtgttg acaaaagaag aaggtggacg tcatactcca ttcttcacta
    26521 actaccgtcc acaattctac ttccgtacaa ctgacgtaac aggtgttgtt gaattaccag
    26581 aaggaactga aatggttatg cctggcgaca acgtaacaat ggaagttgaa ttaatccacc
    26641 caatcgctat cgaaaacggt actaaattct caatccgtga aggcggacgt acagttggtg
    26701 ccggtgtcgt tacagaaatc aaagcttaat tcacttaagt tttaaagtaa agcaacattt
    26761 attcggacgt aatcgctcgg aacgaaggtc ttaggatctt tgttccgagc tttttt
//
LOCUS       DAMEET010000036        24897 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.036, whole genome shotgun sequence.
ACCESSION   DAMEET010000036 DAMEET010000000
VERSION     DAMEET010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 24897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.036"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(88..312)
                     /locus_tag="PB780_001322"
     CDS             complement(88..312)
                     /locus_tag="PB780_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HDE0733386.1"
                     /translation="METEWQIRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(510..2090)
                     /locus_tag="PB780_001323"
     CDS             complement(510..2090)
                     /locus_tag="PB780_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDE0733387.1"
                     /translation="MNNPELKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2491..3867)
                     /locus_tag="PB780_001324"
     CDS             complement(2491..3867)
                     /locus_tag="PB780_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDE0733388.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3995..5113)
                     /locus_tag="PB780_001325"
     CDS             complement(3995..5113)
                     /locus_tag="PB780_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDE0733389.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5255..5535
                     /locus_tag="PB780_001326"
                     /pseudo
     CDS             5255..5535
                     /locus_tag="PB780_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(5613..6146)
                     /locus_tag="PB780_001327"
     CDS             complement(5613..6146)
                     /locus_tag="PB780_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HDE0733390.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6240..7418)
                     /gene="mutY"
                     /locus_tag="PB780_001328"
     CDS             complement(6240..7418)
                     /gene="mutY"
                     /locus_tag="PB780_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDE0733391.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7411..8208)
                     /gene="recX"
                     /locus_tag="PB780_001329"
     CDS             complement(7411..8208)
                     /gene="recX"
                     /locus_tag="PB780_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDE0733392.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNRGKSQKELLRKIQQTLMTKGFSQEVIQQAMADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8315..9697
                     /gene="rlmD"
                     /locus_tag="PB780_001330"
     CDS             8315..9697
                     /gene="rlmD"
                     /locus_tag="PB780_001330"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDE0733393.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMICEINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(9871..10449)
                     /locus_tag="PB780_001331"
     CDS             complement(9871..10449)
                     /locus_tag="PB780_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HDE0733394.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            10771..11241
                     /locus_tag="PB780_001332"
     CDS             10771..11241
                     /locus_tag="PB780_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HDE0733395.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGEYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11349..12575)
                     /locus_tag="PB780_001333"
     CDS             complement(11349..12575)
                     /locus_tag="PB780_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDE0733396.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13329..13529)
                     /locus_tag="PB780_001334"
     CDS             complement(13329..13529)
                     /locus_tag="PB780_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDE0733397.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13663..14271)
                     /locus_tag="PB780_001335"
     CDS             complement(13663..14271)
                     /locus_tag="PB780_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDE0733398.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14356..14826)
                     /locus_tag="PB780_001336"
     CDS             complement(14356..14826)
                     /locus_tag="PB780_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HDE0733399.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(14874..15788)
                     /locus_tag="PB780_001337"
     CDS             complement(14874..15788)
                     /locus_tag="PB780_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDE0733400.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            15934..16737
                     /locus_tag="PB780_001338"
     CDS             15934..16737
                     /locus_tag="PB780_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HDE0733401.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            16915..17862
                     /locus_tag="PB780_001339"
     CDS             16915..17862
                     /locus_tag="PB780_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDE0733402.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(17930..18223)
                     /locus_tag="PB780_001340"
     CDS             complement(17930..18223)
                     /locus_tag="PB780_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HDE0733403.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(18251..18916)
                     /locus_tag="PB780_001341"
     CDS             complement(18251..18916)
                     /locus_tag="PB780_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDE0733404.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(19056..19688)
                     /locus_tag="PB780_001342"
     CDS             complement(19056..19688)
                     /locus_tag="PB780_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDE0733405.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(19695..20762)
                     /locus_tag="PB780_001343"
     CDS             complement(19695..20762)
                     /locus_tag="PB780_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDE0733406.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVETKVPLMKEA"
     gene            complement(20759..21490)
                     /locus_tag="PB780_001344"
     CDS             complement(20759..21490)
                     /locus_tag="PB780_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HDE0733407.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            complement(21658..22137)
                     /gene="greA"
                     /locus_tag="PB780_001345"
     CDS             complement(21658..22137)
                     /gene="greA"
                     /locus_tag="PB780_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDE0733408.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(22273..23448)
                     /gene="mltG"
                     /locus_tag="PB780_001346"
     CDS             complement(22273..23448)
                     /gene="mltG"
                     /locus_tag="PB780_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDE0733409.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            23613..24773
                     /locus_tag="PB780_001347"
     CDS             23613..24773
                     /locus_tag="PB780_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDE0733410.1"
                     /translation="MSKGKHRKKQKISISFLVILLLSVSFFSTGIIIGGQLTKIPHSQ
                     TYKAEPTQLTSNLTPDEQRLASKIDQLLQKSDYIGSIYVRKNNRVLLEKGYGYANLRV
                     KESNSPSIYYQIGSIQKAMTALLILKQIQAGKLTFDTKLAEFYPQIPGSQQITIKNML
                     YMRSGLHRTASPDKPMSDSEIIQFALHHLKLTDYDTYHYEPLNYTLLTGILIKLTNQP
                     YETLLKKEIIRPLHLEHTDFYDNVKNSPQHAVSYQMSATDDYSKALIEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGGIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVVAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVHLSN"
BASE COUNT         7433 a         5061 c         3997 g         8406 t
ORIGIN      
        1 aaattcacga aaattgcttt tcaaattttt gtgaattcca gcttatttct tggagttaaa
       61 cgcttctgtc tcagcctctt tttctttcta gctttttgat ttttctcttt tcttttgtac
      121 ttttctcttt tcattttcag ttctacgcgt gtctttattt ttaacttttt cctctttatg
      181 gatgattgat cgtacttttt gtgcaatttg ctcgtttttg tcccagtctt ttacatctaa
      241 taatcgacat aatcgtaaca tctcttcttt tttctgtttt tttctttcac gtatttgcca
      301 ttctgtttcc atgtgtcaca ctctcctttc tttaatcata tgtacgcaaa ataattttta
      361 tatttttttg attagacatt tctatgattt taatatagta aaaagagccg tcacggaaaa
      421 tccgtcacgg ctctaaacaa aatatgagag gctgtttttg tttggctatt cttattactt
      481 gatgcagggc agcttgtgtc aagacttctt tacatcaaac tttttaattt tacattagga
      541 tatttatccg caaaccagcg ttctgcaaat tggttctcga ataagaacaa tggttggtcg
      601 aaacggtcac gagccaagat attgcgactt gaactcatct tctcatccaa atcttctgga
      661 tcgatccagc gagcgatttt gtgtcccatt ggagacatga ttacttcggc gttatactca
      721 tttaacatac gatattggaa tacttcgaat tgtagctgac caacagctcc aatgatatat
      781 tcttccgtta gatatgtttt ataaagctga atcgctcctt cttgaactaa ttgatagatt
      841 cctttgtgga aggatttttg tttcatgaca ttttttgcag tcactttcat aaacagctct
      901 ggagtaaatg aagggagttc ttcataagca acttttagtt ttccttcgaa caatgtatcc
      961 ccaatctgat aattgcctgt atcatataca ccgatgatat cgcctgctac tgctttttcg
     1021 acgttttctc tcgaatctgc cataaattgt gtgacattgc ttaatttcag ttttttattt
     1081 gtacgttcca accagacatc cattcctcgt tcgaatgttc cagaacagat tcggacaaaa
     1141 gcgattcggt cacgatgggc tggattcata tttgcttgga tcttgaatac gaaacctgaa
     1201 aattcttcct cataaggact taccatctga tcatcttgtg ttttgtgtcc ataaggagct
     1261 ggtgccaatt caacgaaagt ctctaaaaaa gtctgtacac cgaagttagt caatgcagaa
     1321 ccaaaaaata ctggtgtctg atcgccacga gcaatttttt cttgattaaa tgcatctcca
     1381 gcctctttga ttagttctac ttcttctaag acttgttcat ataaggaatt ttcagttaga
     1441 ggatgatttt cgggaagcag tccatcttca tccaattcta ctaatcgttc atcctgataa
     1501 ttttccggac gataaaactc tacacgatta tggtagatat cataaagacc ttctagccct
     1561 ttacccatac cgattggcca attcatcgga taagattcga tatctaatag ctcttctaat
     1621 tcttctaaca attccaatgg ttcacgacca tcacgatcta gtttgttgat aaaagtaaat
     1681 atcgggatgc cacgtttttt tacaacttgg aataatttct ttgtctgtgc ttcgatccct
     1741 tttgcactat cgattaccat gaccgcacta tccacggcca tcaaagtacg ataagtatct
     1801 tcagaaaaat cttcatgtcc tggtgtatcc aagatattta ctcgtttccc ttgataatca
     1861 aactgcataa cagaactagt aacagaaatc ccacgttgtt tctcgatttc catccagtct
     1921 gatttggcaa aattacctgt ttttttgcct tttacggtcc cggcttgacg gatagcccca
     1981 ccaaacaata gtaattgttc tgtaatcgtt gttttcccag catccggatg ggaaatgatc
     2041 gcaaaagttc tgcgattgtc cacttgttgc tttaattctg gattattcat aacctaactc
     2101 catttcttcc attcaattat ttatattttc tctaggcaca gattgtccga tctcttaaag
     2161 aaagacaatt tcatataggg ctgtgacaaa agccgtgtag cagtctcttt ttccgagtaa
     2221 acggcgttct agagacagcc tatcagcaat aagcccaccc aagccaaaaa tacgaaaagc
     2281 tgtttttgct tggctgctct tattacttaa tgcaggatag ctttttcaca acctttgtca
     2341 actactacaa ctgaatcagt atagcatgga ttcatcgaat gaaaaagcca ccaaaccata
     2401 agagtcaaat atttttttgt cagctgacta aaagaaaaga cgtgttgctt gtttattcaa
     2461 gcaacacgtc tttcataacc aagtttccaa ttatcttctt ggctcgtcat cttcgaattc
     2521 ttttctgaag tatcttcgtt cttcttcagg ttccgcttct gtttcttccg gatcatgaaa
     2581 aatgacacgg attttaagga ttcgagaacc ttccatttct tcagaaacca gcgtaatatt
     2641 atcgacatca aacgataact tttcgccttc atccggaatc atgcctaatg ctgtaatcaa
     2701 ataaccagcc atcgtatcta catcactcat atgcagatca ctgtcaaaag cttcattgaa
     2761 ctcatcaatc agcatacgtc cttggatgat atattcgtgt tcatcgattt tttcataaag
     2821 attctcaact tcatctgatt catcatcgat ttctccgacg atttcttcta aaaggtcttc
     2881 tagtgttgct aacccaacga cgccgccata ttcatctaat aaaatagcca tctgattctg
     2941 cgtctttttc atttcatata acagatcatc aataaagacc gtttcaggaa cgaataaagg
     3001 ctcctgcatg attttcttga tatctaaatt atcaaaaccg aatttatggg cagcttttag
     3061 taaattcttt gtatgaagga ttccgactac tttatctttg tcttcgttat aaacagggat
     3121 tcttgaatag ttttcggata aaacttcatt cacattttct tccacagtgt cgttgatatc
     3181 aatcataaat gcatccgttc gtgggaccat gacttctcgg gcaactttcg tatcaagaga
     3241 aaaaacccct tggagcattt ctaattcttc attattcaat acgccttcgt tttcaagcat
     3301 ataacgcatt tcatctctgg tcatttttga atcttcatca tcaaaagtca tcggcgtgat
     3361 acgcgccaat aaacttgtgg aagcagaaag cagccagacg aagggcttag ctactacacc
     3421 aatcaagcgt atcagccctg aagtgaactt tgcgacttct tctgatttat ttaaagcaat
     3481 acgtttaggg tacaattctc caaaaacaat cgatacatag gtcaaaatag ccagcacaat
     3541 gatatttgca atattttttg ccgctgtgcc gccacctaat accggagcta accgttccga
     3601 taaagtatta gctagtgaag caccagacaa aatgttgacc aaagtgattc ctacttggat
     3661 tgtcgatagg aaattatttg gattctgcaa tatttttaaa agcttttgag atttcacatc
     3721 tccttcatca gctttttgct cgattcgatt cttattgatc gaaacaacag caatttcgga
     3781 agctgctaaa aaagcattga tcaatgtcaa aacgatcaat aataaaatct gcgctaacag
     3841 cgactgactc tcagggtcag cattcataat cgcattctct ccttataaaa ttaattttta
     3901 gtttacgata gaaaaaaacg tggcactata gccacgttaa aatggtatca caaagcaatt
     3961 ttctttgcaa taaaaacaag tgtactaaaa ctacctacga ggtcgtagca attttcttct
     4021 tgttttcatt ttgcttgtct tctttgacga ttttgattat ataggaaatg atcgtcttgc
     4081 agattgcgta aaaaggaaca cctaagaaga taccaagcaa tccggcaata tttccagcta
     4141 ctagaagaac gataatgatc gttaatggat ggattttcag tgatttccca attacattag
     4201 gataaatgat attcccatcc aactgttgga cgattagcac aaccacacaa caaagtaatg
     4261 cttttaccgg atcattgaag actgttacta gaaatgcagg tgctagtcct agataaggtc
     4321 ctaaatatgg aattaggttt gtcaatcccg caatgacccc aaataagaaa gcataatcca
     4381 caccaattac catatatcct aaaaatgtga aggtaccaac gaataaacat tcgattgctt
     4441 gtccgctaat gtaattagcc aatgttttat tcagttgccc caataactcc acaatttggt
     4501 ttcttctttt ttctgggaaa aactgttgga tgtttggcac tagtttttct ccgtctttca
     4561 acatatagaa caagatgaat ggtgctgtaa tgattactac ggttgccgaa gcgatcgttc
     4621 caacgatcga acctaaacta ttggatactc cactaaggaa ttgctgaatg attgtcccgt
     4681 aagaaatgtc taattgctca aaatatttat tgatatctac ttccttgaac aaaggcaatt
     4741 cggctacctc ttttaaccag ctttctacct gtttgatgaa atcaggcata ttagatgcca
     4801 atgaggaaat ttgctgtact agacttggga tgacactaag taaaatccaa ataattgctg
     4861 caactaaaag gagcagcaca attgcgacag cccatattct tttgattttt gctttcatca
     4921 acagcttgac aataggattc aataaatagt aaagaaagcc tgcaatcagt acaggtgcaa
     4981 ataacgtgga aaaaaatgtc ccgatcggtt cgaaaataaa gtttatttct gatgaaatca
     5041 tgatcaatgc agctatgatc aataattcaa gcgaccaaaa cattagttta gactgtctaa
     5101 gtttttcaaa caatagattt cctcctttct ttcttactag tatatattat attcgttaaa
     5161 aattctttta ttcttttttt atttttgaaa acttgccata ctttttattt gttttatttt
     5221 ccagtatact atacgaaagg aaggtggcta gaccatgaaa gttgtacaaa caaaagatac
     5281 aatgagcaat atttacttag atgctgtacg gatcaggcat caagtttttg tagtcgaaca
     5341 gggtatgcct ttgtctcgag aaatagacaa agatgaagcc cattgtatcc acttcgtcct
     5401 gtactcagat aaaaaagaac cgcaaggaac tgtacgtctt ttgcctttag aaaacggaaa
     5461 aatgaaatta cagcggatgg cgatcctttt gaagatgctg gcatgcctca tatttatatg
     5521 aaaaagactt tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa
     5581 atctctcact ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg
     5641 ttataccaaa tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca
     5701 accagaatct tcacattttc ttttaggatg aagtcaatat cgttcggata attcatcatt
     5761 ttgctgtgca tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc
     5821 ttgatatcca agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa
     5881 gctagattac aataatagga aacccccttt tctctgatca ttgctattac attgaaccag
     5941 tattttttat gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat
     6001 tcagtgacta atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc
     6061 atggtatctc gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca
     6121 aactctcctt cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg
     6181 tgtttgggtg ttcattacac cgacatcata attattatat caataaaaat gagctttgcc
     6241 tagaaaggat tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc
     6301 ttaggaaaaa cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg
     6361 gtaaattctt gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc
     6421 agatgactaa aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc
     6481 tgcttttcaa atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt
     6541 tctggctttg tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc
     6601 attggaaatg tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac
     6661 gctccagaat gattttgaag agccgcactg atataataaa cgtcttttgg ctttgctttt
     6721 tttgttttta caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg
     6781 atcggacaag tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc
     6841 attgcttggt tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca
     6901 aagattttcc tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact
     6961 actcgcatca cgttgccgtc aaccgcgggt tctggaagtc caaatgcgat acttgcaatc
     7021 gcgcctgtcg tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc
     7081 atttccccat caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt
     7141 gagtaataac caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc
     7201 tcttcaattg taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact
     7261 ctggtttgct gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc
     7321 caaggcaggt ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt
     7381 gtttctttat ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat
     7441 gaatcgctgg atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag
     7501 atttcttttt ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac
     7561 taaataatca tattctattt cttgatctgc ttcttgcgga agatcagcca tcgcttgttg
     7621 gatcacttct tgagaaaaac ctttcgtcat caatgtttgt tggatttttc tcaataattc
     7681 tttttggctt tttccgcgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg
     7741 aacttggtct tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc
     7801 tttttgctgc atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt
     7861 gcgcacataa ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc
     7921 tacgatttta tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc
     7981 tttttccgat cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc
     8041 tccgctactc ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa
     8101 tcgatagcgt actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac
     8161 gcgatagaaa gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct
     8221 cgaacatata ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga
     8281 ataaaccaca gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg
     8341 acaaacggtt cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt
     8401 caaacggttg atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat
     8461 cacgaaagca actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa
     8521 agatcgcgta gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca
     8581 cttagcttat caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa
     8641 attcaaacca gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc
     8701 ttggcattat agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat
     8761 tgaagctggt ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt
     8821 tcaagaacct cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga
     8881 tgcacccatc tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat
     8941 cggtgttaaa actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca
     9001 aaaaaacgca atgatctgtg agatcaatga acgactgccc gaagttgttt caatcatgca
     9061 aaatgtccag gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg
     9121 aaaagaagcc attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt
     9181 ctttcaactc aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga
     9241 tgtccagcct aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag
     9301 tgtagcaaaa caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga
     9361 tgcaaaaaaa aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc
     9421 agagcaagtt ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga
     9481 tccaccacgt acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa
     9541 aaaacttgtt tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag
     9601 taaaaagttc aagattgaat atttacagtc tgtcgacatg tttcctcaga ctgcacgttg
     9661 tgaagttgtc gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc
     9721 tgccctgtat caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata
     9781 ccgtacttgt gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga
     9841 catagttttt gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata
     9901 aaatgcattt gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg
     9961 atcctgcgga agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc
    10021 tatttttgtg atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg
    10081 tttggtaatg tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat
    10141 atttaatgtt cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa
    10201 aggctcaaag ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt
    10261 agcttcgata ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc
    10321 tgctgatgtt cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc
    10381 tggaatgcta aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat
    10441 ctttttcatg tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta
    10501 acacaaacat tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat
    10561 gcagattata acagaagtag acgagaaaag tacaaataga aaacatgaga accaaatcat
    10621 atgatctttt tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa
    10681 tcatcaattt ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt
    10741 aaactactat tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg
    10801 gtttgtgtta taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc
    10861 ggagaatatt actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg
    10921 ccgatgtgga ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac
    10981 aacaacaaaa aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat
    11041 tttatatggt ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt
    11101 acacttttag ttattttctt gtcagtgctt atgtatcagt tgtggctaca tcatcgcctg
    11161 gcaatgtggc tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat
    11221 tcaatttatt ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa
    11281 aagtgccacc taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact
    11341 atttttttct agtacccact tgttgttcca tactcattat tctggtcata accataacca
    11401 ttttgttcct gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac
    11461 tgacttgtat cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg
    11521 ttttctgaat aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc
    11581 gaatcgccat cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta
    11641 ggtaatgtat cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga
    11701 agatcatttt ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc
    11761 aactggtctt gtttgattgt ttcaaacgca ggggtgtagt tcgtcgctac atcaagcatg
    11821 tcatcccaat ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa
    11881 ttgcttacgc catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga
    11941 cgacgttgtc gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa
    12001 cctttttctc cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag
    12061 tcaattttat tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc
    12121 aaattgatcg ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg
    12181 gccatttcga caccaccaaa tgcataggca tgatttaatt tatctaccgt tccataacct
    12241 acgatattcg tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga
    12301 ttaactgtta ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg
    12361 gtatctaaac ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca
    12421 gaacgtttgg aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct
    12481 tctcccataa atctgattcc gtatgctgat aatccggcaa tcagcaaaac aaatacccct
    12541 aacaaggtta atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct
    12601 aaaatattgt ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact
    12661 ttaaaaaaca tttttgtgta acgattacta ttttttaaaa tttctcaaaa ttcttaacta
    12721 tcataccata ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat
    12781 ctttttttac gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg
    12841 cagtaaaatg atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga
    12901 ttatcattcg atttcacgaa aatttcttcc tatgactggt ccttttgtta aacccatctt
    12961 ctattcgttt tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgatttc
    13021 attaccatca tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt
    13081 aaagtaatag aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt
    13141 tattcaacat ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc
    13201 ttgcttgata attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaaat
    13261 ctaaaaactt cataaataga caaaaagaca cgactctcta agaagtcgtg tctttattca
    13321 acaaattatt ataatttagt cacttcagca gcttgaggtc cgcgtgcacc ttcaacaatc
    13381 gtgaattcta cttcttgacc ttcttctagg ctcttgaagc cgtctccttg aattgctgag
    13441 aaatgaacaa atacgtcatc tccgccgtct actgtaataa acccaaatcc tttttcattg
    13501 ttgaaccatt taaccgttcc gtgttccata aatgtatagc ctccaaaaaa gttcttgcaa
    13561 gtaaccttgc aagaacatta tacccaattt agaaaacgct agcaagcttt cgctctttta
    13621 ttttccaaat tatttttaag actgatatga cggaggaaat ggttattttg ctgcttcgaa
    13681 acgtttgttt acttcatccc aattaataac attccagaat gctgcaatat aatctggtcg
    13741 gacattttta tatttcaaat aataagcatg ttcccaaacg tccaaaccta gaattggtgt
    13801 ctttccttcc atcaatggag aatcttgatt tgcagtagag gtaatagcta attttccatt
    13861 ttccattaca agccaagccc atccagaacc aaatcgtcca gcggctgctt ttttgaattc
    13921 ttctttaaaa gaagaaaaat caccaaaagc ttcattaatc gcttctttta tttctcctgt
    13981 aggttcgcca ccagcatttg gtgccatgat ttcccagaaa aatgaatggt tagcatgtcc
    14041 gccaccattg ttacgtacag ctgtcttgat atctgttgga atagcgtcca tatcagataa
    14101 taattcttct attgtttttt cgcctaattc tgggtatttc tcaattgctg catttaaatt
    14161 cgtcacataa gtattgtgat gcttatcatg atgcagatgc atcgtttctt cgtcaatata
    14221 aggttccaat gcgtcatatg cataaggtag atctggtaat gtgtaagtca taaaaaaatt
    14281 cctcctcaat gttttcttta taatgtctac attacaaaga gtaacaaaga gaaggaaatt
    14341 cttcaattgt tatgcctagt cgtctttttc actttgacct acttcttctt caaaaacctc
    14401 agtttcttta aaaataaaca acttgctgaa aatatagttc gctaggataa caataatttg
    14461 agtgatgatt ttcgccacat actcattgct gttcataaca ttgatcaaca ataccattgc
    14521 ccccatgtcc aatccgtaag aaaggatacg gtagaagaag aacttaccgc attcaataag
    14581 tgcatgtgta aaattcggtg attttgaatg gaatacccaa agtttgttcg ttacaaaagc
    14641 aaacaataca gaacaaaacc acgaaataga attggataag gccatactta aatcaaaata
    14701 ttggcgtgcg agagcaaaac taacaaaatt tacaatcgta gccaaaccac caaaaaagag
    14761 atagactagt atctcccaat atccttttgc ttctaaatat ccttttaact gtcgatactg
    14821 cttcatgagc caacctacct tattaaaaat tcttatttat catactctat atttcaataa
    14881 ctttcatctt tatttaaaaa aattaatttt ttttgatcct ttgcctttgt ctctgcttgg
    14941 atcgtcacat gtgtaatacc atattttaat tttaataaag aaattatacg aggatatatc
    15001 ttttcaatct cgcttagtag ctgatcttca aaatcaatat gtccgtcgaa aataattatc
    15061 ttttcgctat actgccaaat atggacatga tgaacattct ttaccccatc gattgcttca
    15121 atttcttgtt ccattttttc atagtcaata gcaggtgcac tttgtatcaa aatagcggca
    15181 gcttttttga tcaccattat cgcttctatc aatatatata tcgaaatgat cagtgtaata
    15241 actggatcga tccagtagat atcgaaaaaa cggatcaaaa ttgctccgat aatcacactt
    15301 accgaagaca gtgtatcact taacaaatgc aaataactag attttatatt caaactttca
    15361 tctgcttctt gtcgtaataa taatactgag aacaaattcg cgagcaatcc aataactgct
    15421 actgttagca tcaaatgact atttatcttt tctggtgaag tgaaccgctt gaatgcttca
    15481 acggttaaaa acaaggataa tatgatcaat atgaatgagt tgatgaaagc actcaatatt
    15541 tctaaacgct ggtagccata cgtacgtttt ttatttgctt cttttcgggc cattttttgt
    15601 gccccgtaag aaaaaataat tgccaatgta tcactaaggt tatggaaagc atctgaaaca
    15661 agagacaaac tccctgacat aatccctcca ataaattctg ataaagtgat caccgcattc
    15721 aaaaccacta ctccaaatag tttttttgat gatctctgtg gctgtgtagg cactattcct
    15781 tcctccattt tatttatatt ggatagtctt tagacaagaa taacattttt ttgatatttc
    15841 accaatcatt cagatgtttc cctaaagcac ttgtatttca cttgatactt cgctagaatt
    15901 gcaagtgatg acactactag atgaggagta cttatgacaa caaaacaatt aattatcagt
    15961 tcctttatac gattcaaaga cctaaaggag gcgagaaatg tcccattttg gaaagccatt
    16021 gtctatcttt tagttttgag tataattatg gctttgccaa tctcttttca agtttttcaa
    16081 gtcttaaata acattcaatt ggatgggcaa aagattgcta ctcgcattcc tgattttaca
    16141 atccaaaacg ggcagcttca aaccgaagaa aaagaaggat tcatttacca aacaaactcc
    16201 attatcttca catttgatcc agagggaaaa cgaacagaac aggacatctc tactgatttg
    16261 atggggaatt ttgttagtgt cgggatgtta aaaaacaaac ttatcatagc tcttcctaat
    16321 acaggcacga cttctgcttt gttgaacaat aaccaattag aacttcccta caccaatgaa
    16381 tctttaaaaa acttaacagg taagcagctc cgttcttttt taagtgaagc atctatacca
    16441 ctttggataa aagtcctgac atttatattt tctatttatc ctagttttct gaatctgctg
    16501 atcactttgc tatttaccaa cattgctgct tttctctatg cacgctttcg actaacaaaa
    16561 gctacttttc ttgattgttt aaaaacattg atctattcaa tttcattgcc tactataatt
    16621 gcaacaatct tgatggtctt tcttccttct tttgacacta gcgccttcat tgcttttgca
    16681 ggaatcttta tttttgctca agctgtcaaa ggatgggcaa aaatatcgat caattaagaa
    16741 ataaagacaa ctcgttattt ctacttaaat agaccctttt agaggttgtg acattttttt
    16801 gtcacagccc cttttttatt tgaaaaatat ttctttctca aaaaacaagc aaggcgcttc
    16861 aaagttcttc ttgtatgcgc tacacttaga gtaaagaaag gcgggaaatt aactatgtca
    16921 gaaagaatca tttatgcaga tcgtccattt cgtgatgaat ttatcgaaga aatccatact
    16981 atagcgcctg gttatatctt taaaacagaa ttacaaccag aggatctaca aaatgtagag
    17041 atcagtttgg gctggaactc agactaccaa gaaaaactat tagcctcttc taccttgaaa
    17101 tgggtccaat ctatctcagc tggtgttgat cagctacccc ttgagacttt ttcaaagaaa
    17161 catattttac tttcaaatgg tagtggcatc cacagtgaat cgattacgga gcatgtctta
    17221 ggaatcatcc ttgggtattc tcgtggtctt tttcaagcac aaaaggcaca gagtgacatg
    17281 aaatggttag gttcatcggt ccattaccaa tctatccaag ggaaaaaaat actaatcatt
    17341 ggaactggcc atattggaaa aatgattgca aaaaaatgca aagcattaga catgaactgc
    17401 tatggaatca atacatccgg tcatccagca gaaggaatgg aaaagacttt ttctttaaat
    17461 caactaaagg aagttcttcc tgaagtagat ttcgtagtca atatcttacc tctaacggag
    17521 gaaacaactg ggctctttga tcagcatatg tttgaacact ttgatccaaa atctgtattc
    17581 atcaatgttg gccgtggtgc ttctgttaag acacaagatc tggtgcaggc tttaaataac
    17641 cagcaacttt cctttgcagc attagacgtt tttgaggaag agcctcttcc acaagatcat
    17701 cctctttgga aaatggacaa tgtactgatt acttcccaca ttgcaggttt gacacctgat
    17761 tttcaaaaga aattaatggc tattttttta accaatctga aaagctactt tgccacccat
    17821 gagcttcaaa ccaatcaagt aaaattgtct gcaggatatt aatagctgaa aacaatattt
    17881 tcaaaagaaa aaagagaagg tgacaaaaat tgtgtcaccc tctctttttt tattgttttt
    17941 taaagtgata ggttggctcc tccaggttgg gatcataatc aacaagtaaa tcatatcctt
    18001 taaagaacca ttcatctgct tcctcgataa aaaaaagtgt cccatccatt tccactttct
    18061 ttacaggatg ttctggctgt tcaactgaca ttccaatcga aaaaccatca tgaacctctg
    18121 ttttgccgta gacttttcca aaaaaacgta ttccgtaagt ttctggcaga tcaatttctg
    18181 agtcaaacca tttttgtgct tttggcgtta ccgttaattt catcccaaca acctactttc
    18241 tcttttcttt ctattgattc atcttatcgt ctagaatatc cacatcgcct gtctctccaa
    18301 taaccagtag attatcattt tcacgaacat attcatctgc tgcaggagaa gcgataaccg
    18361 tttgatctac tcttcgtatg gcgactactg ttaagtcaaa acgttgcctg aaatttaatt
    18421 cagctaatgt tttgttgaaa aatttaggat tggagactcg gatttctgct aaagaatatt
    18481 tatctgacag ctctaagtag tctagaatat tctttgatac taagttatgt gcaatccgta
    18541 tccccatgtc tcgctcaggg tgtacgacac gatctgctcc gatcttctct agtacacggg
    18601 catggtactc attttgggct tttgctaaaa tattcggtac gcccatttcc tttaccatca
    18661 gagtaactaa gatgctggct tggatatctt ctccaatagc gacaattaca tgatcgaaat
    18721 ttcttatacc taatgaacga agtgtcatct catcctgtgc atttgccaca acagcatggg
    18781 tggctatatt catatattca ttgactcgat cttcattact gtcaatagcc agtacttctt
    18841 gtccagcttc gatcagtgta cggcagatgc ttccgccaaa tcgtccaagg cctacaattg
    18901 caaaattttg tttcaccatt ttcctccttt agtatcttct tttctcagta taacaaagcc
    18961 tttccaaaaa caaaagcaag catcattatt gtaaaaaagc caagaaaaat aatagttatt
    19021 ttctcagctt ttgattatcc tctttatcat cgtttttatt tcaccaaacc atgtttaaac
    19081 gcatagatag ctgcttgagt tcggtcttct acctctaact tagctaatat attcgacaca
    19141 tgcgttttga ctgttttcag tgtgataaac aattcatccg cgatttcctg attacttttc
    19201 ccttcagaaa tcagcatcaa aatttcattt tctcgattcg ttaattcttc atgcaatact
    19261 ggatcatttc gccgactcat tttttccatc atttttgttg tcacttcagg ttctagtact
    19321 cgctcgccgc gttgcgtagc ccttatagca tcagctattt catgtgctgt agacgtcttc
    19381 aacaaatatc ctgctgctcc tgcttcaatt gcaggataaa ctttttcatc atcaataaaa
    19441 cttgtgacga taatgatttt ggcttttggc caatctttta agattgcttt tgttgattcg
    19501 atgccatcca tttcttccat gactagatcc attaaaatga catccggtcg aagtgccatc
    19561 gccttttcat agccttgacg tccattttca gcttctccaa tcacttcaat gtcttcttgg
    19621 atagataaat aagaagagac gcctaagcgt accatttcat ggtcatctac taataaaact
    19681 tttatcatct ttatctatgc ctccttcatc aaagggacct tggtctccac gctcgtacct
    19741 tgccctttaa aactaatgat ttttactgtt ccaccaatac cagctactcg ttctttgata
    19801 ttgttcaatc cataacttcc cgttttggtt tcattcatat taaatcctgt cccgtcgtca
    19861 atgatacgta ataaaagatt gttatctatt ttatgcaaat agacttctaa ttcattagcc
    19921 ttagcatgtc ttaatgtgtt tgataacaat tcttgaacga ttcggaacaa gtgatcctca
    19981 atggaactag ttaattttac atcttctacc tcccatttta gcgaaatctg gattttattc
    20041 tgcaattcct tcaataactg ctcaatgcct tgttttaaac ttttttcttc taaattgact
    20101 ggacgcagat gaagaagcag cgcacgcatc tcagactggg aagcgttgat gatctctgcc
    20161 accattttca actgcttacg gaacatttct ggtgtctcac ttttttctgc ttgctccgtc
    20221 aatgccgaca tcatcatcat cgctgcaaat aattgctgtg aaactgaatc atgaagctca
    20281 cgagctaatc gatgcctttc taattttaaa atctcttctt tggtttgtcc atctacatat
    20341 tgaggacggc tagtcataat ctgtaattca ctggatactt cgatcatttt ttcttttatt
    20401 ttagtaattt ccatatcaat atctttgatg tacagatcat ccgacgcgct tgggatcggc
    20461 tgatgcaaaa gtttggattc ataattgcca gaagaaagtg cacgaagctt ttcttcgatt
    20521 ttcccgtatt ttgccttttg aataagtgat aacaatagaa atacgatcaa tcctacacct
    20581 aatgaaattg ccatcaaatg gaagataagc ggaacgtata ataaacgcgc acgcagcaat
    20641 tctccccacc aatgactttg gttactggca taaaaataag tgaaaagtga aaatagaata
    20701 ataaggaaag ccgcaatccc cgaataaaca gctagcattg ctttggatat tcttcccatc
    20761 atattcgtat cacctcgaca tctccaacca aagtattggt aatgatcttc aatctgcgag
    20821 gattttcatc ataatcactg ctgcagattt tgatctgttc gttttttaag gaaaactgct
    20881 cttcttcaaa caagacattt cctactaatg tcgcatgctc taaacgaata gccactccca
    20941 ggggtactaa gatccgtgtt ctgcctatcc cttttcttac aatcacaata ttatcatctt
    21001 ttggcaaaag tgtattgccc aaatcgatga tagtatcacc agaaatgatg gcaatgttga
    21061 tatcatccca ttcatagacg tcgtttccaa tacgttggtt accaaataat tgctgtcttt
    21121 tacgctcatt attatgtgtt tttaattgct ctgtttggac catcatgatc tgtttttttc
    21181 gccaaaaagc attttttgtc aaatctattc ctgaaatctc tacacctttc agcccgataa
    21241 ataaaacggc caatacaacc atcatccata aagcaggact gttgactaag ctaaaaaaaa
    21301 tgaccaagct ccctaagatc aattgaaaat tttgaaattt agtacgagga tatttgcgca
    21361 tagctagata tatattgaaa ataccgaata ttactaaaag caaaagtcca gtattgttta
    21421 cgatttgcca aacagcaaat aacagcagca gtgcttcgac taccacaaaa aaacgccaag
    21481 aactattcat cgctttttct ccctcctgac acattctcat ttcatcatac ctattttttt
    21541 gactgttggc tataggactt tagatgtaag atcgttcagt gaaacctgca atactcaaag
    21601 agaaaagatc tagagtgcgt catcagtaag atgaactctc tagatctttc agtcagacta
    21661 cgcttgttct actttgacga ttttaactaa catatcgcca cctggtgtag caatggttac
    21721 ttcatcattt ttacgtttgc caatcaatgc ttgcgcgata ggagaatcat tggaaatctt
    21781 tccagaaaac gggtctgctt cagcacttcc gacaatcgtg tactcttctt catctccatc
    21841 aggcaactcg atgaacgtaa ctgttttacc gatcgatacc tcatctgcat ctacgccgtc
    21901 attatcgatg atttgtgcaa aacggatcat attttcaatt gttgtgattc ttccttcaac
    21961 aaatgcttgt tcatctttag ctgattcgta ttcggagttt tcggaaagat ctccgaaacc
    22021 gcgagcgatt ttgattcgtt caatgatttc tccgcgttta actgttttta gttcttctaa
    22081 ttctttttct aatttttctt taccttctag agtcattgga taaactttct ctaccatgtg
    22141 taacgtctcc ttttttcgat aaaataccgc aaaaggtaat cttttgagga atatagtcca
    22201 aaaaagatta ccacgctttt cttaaaatgt aaatgttttt ctaaaaatga tcaatgacta
    22261 gcccctgttt ttctaagaat tatttacata tttttgcact agttccatat gttcttcata
    22321 cgttttagcg aaataaacat ttccagtgct cgtatctgct acgaaataat aatagtcatt
    22381 ttctgctggt tctaatactg ccttgattgc ctcttcgctt ggattgtcaa aaggaccagg
    22441 cccatagccg gtattggtat acaaattgta tggtgaatct acagctgtat cttcatacgt
    22501 caccaattct ttatgttctc ctaatgcata taaaacagag atatctgact gaagtggcat
    22561 atttgccttg attcggttaa agaagacttg tgcaatattt ttacgatcat tttcttttac
    22621 gccttctttt tcaactagag aagccaaagt taaaacttct tgtacagtca aatttttctg
    22681 tttgatagct gaataatagt tttgcatgac cgtatttgtt ttgttcacca tctcaatcac
    22741 aagatcctta agcgaagtac tcgtgtagta atcataagta gctgggaata agtagccttc
    22801 caaacggtat tttgtgttct cagctttaga ggcactttcc aacaattctg ggaatttttg
    22861 atacaactcg ttaaagaaag tctcatcatt tactaaatct aaaaattctt tcttgtcttt
    22921 accagttacc ttcgctaccc gctcagcaat ctgatcgatg tcgtagcctt ctggaatggc
    22981 aattttccca tctgcaactt ttgttggctc agaagtgccg ccttcttgca gctgtttgcc
    23041 aatctcgtca agagtcatat taggcgctaa ctgataatag ccagcttgaa agccagttaa
    23101 atttttaaat tttgtataat aattgaaaac aatcccgctt ttaataatat tgtccttctc
    23161 caaaatctca ccaatctgct tgttagaaga gccactcggg atctccactt ggactaattg
    23221 gtcatctgat ttatctaaag gcttcaaccc gctatctaca taacgataca cggtaaatcc
    23281 aagaaatccg ccaataatta ataatgtaag agcaatcact aaaatgatct tcctcacaat
    23341 cttatcttct tttttacgca gatttctccg cttgtcgatt atatctgctg tagctgcctt
    23401 actggcttct tttttatctt tacttgggtg ttgtttgttt cctgacaaaa caactcctcc
    23461 ttactatcat cttcgtaact ctgtttatta tacatgaaat ttctactgcc gtgaattatt
    23521 ttaccaaata ttatgtcaaa attgtaagtg gcaaatactc tttttgagga ttacaatgaa
    23581 aaatagttaa aaatggatag aaaggaatta acatgtcaaa aggaaaacat cgaaaaaaac
    23641 aaaaaatttc tatttctttt cttgtaattt tattattgtc tgtcagcttc ttttcaacag
    23701 gcatcattat aggaggtcaa ttaacaaaaa tacctcattc ccaaacttat aaagcggagc
    23761 caacacaact tacttccaat ttaacgcccg atgagcagag gcttgctagc aaaattgatc
    23821 aattactaca aaaatcagat tatatcggtt cgatttatgt tcgcaaaaat aatcgcgtac
    23881 tattagaaaa aggatatgga tatgcaaatc tgcgcgtaaa agaatcgaat tcaccttcta
    23941 tttactatca aatcggttcg attcaaaaag caatgactgc tttactgata ctaaaacaaa
    24001 tccaagctgg taagttaact tttgacacaa agttagcaga attttatcct cagattcctg
    24061 gaagtcagca aatcacaatc aaaaatatgc tttatatgcg ctctggtctt catcgaactg
    24121 cttctcccga caaacctatg tcggatagtg aaatcattca gttcgccctt catcatttga
    24181 aacttactga ttatgatacg tatcattatg aaccattgaa ttatacattg cttacaggga
    24241 tattgatcaa actaacgaat cagccttatg aaactcttct aaaaaaagaa atcattcgtc
    24301 cactccatct agaacataca gatttttatg acaatgtaaa aaattcgcct caacatgctg
    24361 tatcttacca gatgtctgca actgacgatt attcaaaagc attgatcgag cctgaaaccg
    24421 acatccgaaa tgaattaggt acaggaaaca taagtatgtc tgtttatgac ttgaacaaat
    24481 tctttaccag tgttctttct ggcggtatca taccaaaaga actacttttc tcattgtggc
    24541 aaaataatgg tgataaacat ccttacagcg gcggtgttta cagtggaaac gattatatcc
    24601 ttgctcaggg gaatatcaat cgattccatg cagtagtggc gatgaaaaaa gacactaaag
    24661 atgcaatcgt catggaaagt aatatccaat cggataagaa catcaaattg ccggccactg
    24721 atttaagaaa tcagatttat gaactgatgg aaggtgttca cttatcaaat taaattttga
    24781 cataatatta gttgtcatct ctgtatagtc aatttagtta gtactctatc tgaaaagtag
    24841 aaataaatta acaaatatta tctgtcctta ttatttgtta atttatttct tatttta
//
LOCUS       DAMEET010000037        24747 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.037, whole genome shotgun sequence.
ACCESSION   DAMEET010000037 DAMEET010000000
VERSION     DAMEET010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 24747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.037"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(57..173)
                     /locus_tag="PB780_001348"
     CDS             complement(57..173)
                     /locus_tag="PB780_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HDE0733411.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            complement(418..1332)
                     /locus_tag="PB780_001349"
     CDS             complement(418..1332)
                     /locus_tag="PB780_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDE0733412.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            complement(1347..2348)
                     /locus_tag="PB780_001350"
     CDS             complement(1347..2348)
                     /locus_tag="PB780_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDE0733413.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            complement(2345..4528)
                     /locus_tag="PB780_001351"
     CDS             complement(2345..4528)
                     /locus_tag="PB780_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDE0733414.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            complement(4531..6978)
                     /locus_tag="PB780_001352"
     CDS             complement(4531..6978)
                     /locus_tag="PB780_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDE0733415.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            complement(6962..7354)
                     /locus_tag="PB780_001353"
     CDS             complement(6962..7354)
                     /locus_tag="PB780_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDE0733416.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            complement(7441..7938)
                     /locus_tag="PB780_001354"
     CDS             complement(7441..7938)
                     /locus_tag="PB780_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDE0733417.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            complement(8055..8279)
                     /locus_tag="PB780_001355"
     CDS             complement(8055..8279)
                     /locus_tag="PB780_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733418.1"
                     /translation="MSGTYLSIYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFL
                     IIAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            complement(8383..10293)
                     /gene="ltrA"
                     /locus_tag="PB780_001356"
     CDS             complement(8383..10293)
                     /gene="ltrA"
                     /locus_tag="PB780_001356"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDE0733419.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(11077..12282)
                     /locus_tag="PB780_001357"
     CDS             complement(11077..12282)
                     /locus_tag="PB780_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDE0733420.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            complement(12305..12458)
                     /locus_tag="PB780_001358"
                     /pseudo
     CDS             complement(12305..12458)
                     /locus_tag="PB780_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(12461..13846)
                     /locus_tag="PB780_001359"
     CDS             complement(12461..13846)
                     /locus_tag="PB780_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDE0733421.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            complement(13875..14261)
                     /locus_tag="PB780_001360"
     CDS             complement(13875..14261)
                     /locus_tag="PB780_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDE0733422.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            complement(14277..14591)
                     /locus_tag="PB780_001361"
     CDS             complement(14277..14591)
                     /locus_tag="PB780_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDE0733423.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            complement(14944..15423)
                     /gene="rlmH"
                     /locus_tag="PB780_001362"
     CDS             complement(14944..15423)
                     /gene="rlmH"
                     /locus_tag="PB780_001362"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDE0733424.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            15851..17140
                     /locus_tag="PB780_001363"
     CDS             15851..17140
                     /locus_tag="PB780_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDE0733425.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(17179..17784)
                     /locus_tag="PB780_001364"
     CDS             complement(17179..17784)
                     /locus_tag="PB780_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HDE0733426.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(17980..18444)
                     /locus_tag="PB780_001365"
     CDS             complement(17980..18444)
                     /locus_tag="PB780_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDE0733427.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(18466..18786)
                     /locus_tag="PB780_001366"
     CDS             complement(18466..18786)
                     /locus_tag="PB780_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDE0733428.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            complement(18929..20008)
                     /gene="pepA"
                     /locus_tag="PB780_001367"
     CDS             complement(18929..20008)
                     /gene="pepA"
                     /locus_tag="PB780_001367"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HDE0733429.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            20208..20558
                     /locus_tag="PB780_001368"
     CDS             20208..20558
                     /locus_tag="PB780_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDE0733430.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            20743..21696
                     /locus_tag="PB780_001369"
     CDS             20743..21696
                     /locus_tag="PB780_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDE0733431.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            21709..23496
                     /locus_tag="PB780_001370"
     CDS             21709..23496
                     /locus_tag="PB780_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733432.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            23483..24685
                     /locus_tag="PB780_001371"
     CDS             23483..24685
                     /locus_tag="PB780_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733433.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
BASE COUNT         6992 a         5007 c         4518 g         8230 t
ORIGIN      
        1 gcatcaacat gagctaaaac tccaatatta ataattttca tgtgattttc ctccattcaa
       61 aagcccaaaa gggcataaaa atcccagtga taaatactct tatcactggg atttttatgc
      121 ataaccatag gcatacaaag catacagata ttctccggat actttagaat cacatgataa
      181 aggtattctt aaactgggtg caaaaaacta agccctccta aaaaaggaca tccaattatt
      241 tgttcccact atcaaattga cagtttattt aagaatacct tgccgcatat ttattaactc
      301 cttttaaata gatacttaaa taatagcacg taagagcata tttgtaaagg aatctccaat
      361 tttttatcaa agagagtacg tgattacaaa atagctgtaa taatgtacca atatttgtta
      421 ttctataatc ttccaattac tcccgttctt ttcaagtacc aaatcaaatt gagatacctg
      481 cgttgctttg gtctgctggt cgatatactc cactgtcagc gataccgtga cttgattatc
      541 cttacgattg taaataggat ttaccagttc ttgaaagatg tactcttttc cgattggttt
      601 taatatcccg tcattcacat agtaggaaag ttcactggct gtcgctgtag gatagagctt
      661 gaagaacgtc gttaaaaact cattgatttc attggttgta atggaatcaa ccgtaccctc
      721 actttcaata gcttttggtt tatagtctga tttctttggt atgctagtaa tggtcggatt
      781 cttaatgagg accatatttc cagtactgtc tacatagaca ctcactatat aagcagagtg
      841 gacggtcttt gtattttccc cctctgtaat gagttggtct acactgtagg ttacatcaaa
      901 ttgattgtcg ccatctgctt ctaccgtcca tatctgaaat ccttttacag aagacgatac
      961 aggaatatct ttacgaactg tatcgacatt cagagcttga agttcctctg tcagatatgc
     1021 tttcaggttt tctatgcgat tatcaatgga cttgtcgttt agttcccatg aatagtagac
     1081 tttcgcaaag ttctctacaa aattttccac atgatgagta tcgacatatt ccttttctat
     1141 gatggtggtt tcatgaatgg tatgggtatc aatggctgta aagtgcttga atatcgcaaa
     1201 gctgaaacta agtcctaaaa gtacccatag gacaatcaca acctttttac gaggattgac
     1261 cttatagaca cgaggtttct tttcctttgg tgtctgtttt tctgtattct gattttttcc
     1321 gaatttcatc attgaatcgt cctttcttat tgtttgattc gtcctgctcc cactaaatgc
     1381 tgttgccagt aagagcttgt taagtcggca taaccgatag ggtcgcctgc atgaaacata
     1441 cggttatcgc ctaaataaat cccgatatga gtaatataag agccagcgtt ataggtagaa
     1501 tgaaagaaaa ccaaatcgcc ggcttttgct tccaataatg ggatatgccg ggttacatca
     1561 tattgctgtt gtgctgttcg tggtaagttg attccagctt ttccatacgt ccattgtgtc
     1621 agtccgctac aatcaaaaga ggtagtcggg gaagctccgc catagacata cttccagcct
     1681 tcaaatttca gagcttcgtc catgatggct tgtactgtat catcatcaaa ctctgttgtg
     1741 acaagatact gcgttaccag ttgcacataa aacatattcc cgtaattgta tcgccagccc
     1801 ccattgatag gtatggctat gggattgggg taagacactt tttcgtcacc cgaatactct
     1861 tttgagaaac tttgagccag ttcaaaggta tatttattcc cacgattagc cacatatcct
     1921 aagaaaccac caccataatt gtaggactgg ataaccgatt ctaaatctac accaagcctt
     1981 tcgctactgg ctaataactc actgaaatac ttcacacctt gcttaatgga ttcttctgta
     2041 ctcaatgaat taggtggaag accgagggat tccgaggact gcataacatc ttctgctgta
     2101 ccacccgatt ccacctgtat aatcgcaaga agtatattga cgtattcttc aattccatat
     2161 tctttggcaa ctttttctac cataggctta tgagccagca cttctgcgga aacatccaca
     2221 ccgccatagt gaatgttgga aaaaccgcca tcttgttcat ctgaaaataa aatggcgaca
     2281 aacagaagca gtgagaagac catcaagaat aatcccgaac caccaatcac taaagttttc
     2341 agcttcatgg tttcttactg cctttcttaa tggtggcggt tttgattggt ggtctacttt
     2401 ttgtattttg tagtggtact ctttgaacag tagactgacg ttcttttgtg attgaacgtt
     2461 gtgaagctct atctgttggt gcagttgaag ttactggctt ttgaaccgtt ctttcttgaa
     2521 ctgtattact ttgacgttcc gtttttaaac ttgaagaatc ggacttaact gttggacgct
     2581 cttgtttggc ttgttgagat tccctatagg aagcctgtac agttgtttgc cttgaagtct
     2641 gtccatcatg agattgttct tgcttagtgg ctggtctttc atgaacggaa gaagctggct
     2701 gttttttctg tttgacctgt tccatttcag agcgacgctc cgcaatggtt ttccgtcttt
     2761 gttcctgctg tttcttgcgt ccactggctt tatccgcttt ggtttgagaa atacttctgg
     2821 ttaaatcatg gacattatcc gtcactttag attttccttg atatactgca tatcttgcat
     2881 tggtcggcaa atccttaacc tgttctttca aattaccagc agtatctacc attctgtctt
     2941 tggtatcagc tactgtaccg atggtttgac cgatacgctt tcctagtgtt gatttttcct
     3001 gcccgtttgg tcgagagtga tctgcttgtg tactcgcaga acttcccgaa cccgactgtc
     3061 cttttttacc cgtagcactg gcaatggcag acccagcccc taaagcagtc acggagcgtc
     3121 caagtttccg ctgtagacgg tgcatgtgag cgtgcataag catacgaggt tttctcatca
     3181 tacgacttcc cacactttga gaatcgttac tctgtagaga aaacatactc attaaatcgc
     3241 ccagcttaaa gtagattcct gcaaaggtca caatctgtag aaaagcaatc aaaaagaacg
     3301 gataaccagc cgataaggta tagagcatgg ttgaaatact aaatgctgtc gtaataatca
     3361 atgtgattcc agctcgtgtc aaaatggtat taaagagctt tgttatggct cgttttgaca
     3421 taccatcaaa tgatggaatc atgcttaaaa taaagctcac aggcagaaac atagcataga
     3481 tgataaaaag tacctgcgag aaaatcatga ttcctgttaa taggaataca aatatggaaa
     3541 tcccaatatt gaagacaaat aggaagaaga ctgtacctaa acggttaatc gtctttgtaa
     3601 tcgtcatatt ggtattgctt ctatcttcaa tttcttccgc aacaattttt tctctgtctt
     3661 ccccattgtt ggaatctgga ctggttgaga gcaggctttc cacacggtca ataccaatac
     3721 tttcaatgtc tgaactgtta tattgaagca gtagccacgg ttgctgaacc tgtatggaaa
     3781 ataggctgtc acgtatcaag tccacactgt ccttgccttg actatctgaa tggggcatga
     3841 caatttttgt acccagtgac aaactagcgt tgctgatgtc tgatgaaaag tcattgattt
     3901 ttttaatgta gtcgggagcg taggcaataa aggaagccga taggataaaa accagcacaa
     3961 aattcatgag ggcatgaatt gcctttgtgg tttctctctt tatcagtcct gtataggcaa
     4021 cataaacccc aagaaccaaa atcaagagta aaaggaatcc aacatagaaa ccctctgttg
     4081 atagtccact aggcgtaacc cctgcaaggg tctgcatatt cttgccaatg gaatctgctg
     4141 tagcggaaat gaagtctaag gaataggctt cctgtactaa gtaacccgtc gcattggaaa
     4201 cataaagact gattgtccaa ataaaattgg taatggcata tagtccatac atgacttgtt
     4261 ttccaattcc gtccgaccag ttccacggta gccagcccca gctattatcc acataaaaat
     4321 ccagttgata gttttcaagt gggtatcggc tgtattcatt tgccacattg accgtatcat
     4381 ctaccaagcc cgcagcttga accaccgttc ccagcatggc taaaagaaaa atggcaatca
     4441 caagtgtgaa agccactgtc attgccactt tacctagacg tttcagcgtc cagtttgatt
     4501 ttattctgtt taatattgat ggtttcacat ttacacctct tttcgcacag gtggtctggt
     4561 atcaaaggca tggagcagtt cttcaaatac agggtggaac tgtatcacac cgacacgacc
     4621 atataaatca ctgataaggc attgcccgtt ttccaaatca cgcaatcgct tctgattgtt
     4681 ttcgtcctct gggtctacac caaaaaaggc aagggttttt ttaatctcgt taaggtcagt
     4741 ggaacgaaag gcaaatttta agccgaggtt atttttcagt ttttcatcta agaggtcatc
     4801 tgtattttgg gtcacgaaat ataccccagc gttcatagca cgaccagcac gaaccagctt
     4861 catagatagt gttttccctt gtgccacctg taagaagctc catgcttcgt ctaagtctac
     4921 aatcttgaaa atacttcggt ctgtatggat aaagtccaac gcaaaggtac taatgacaat
     4981 cagcattgct accgatagta actccattgt ggtatattcc tcaaaggaag tttccttgtc
     5041 aggaagtacc aagtcggcaa cctgtataat gttcagttgt ttttcaaggc tgatagactg
     5101 ctctacataa ccatcactaa aaagcagatg ggcaaagtca tagtctgtaa aactttcgat
     5161 atggtcggct atgctggtac ttagtggcgt attctcaacc cgtaattctt caatcacttt
     5221 catcaaccct cgcacttcac tattggttac tgcacgaatg gcttttctaa ggattgggaa
     5281 gcgttcccca tcacgagagg aaatccccgt aaggaatgtc aaaatatcaa tagccagtga
     5341 ttcagaatct ttggtatttt tcataattac ataagggtca agtaagcctt tgtttttctc
     5401 atcagaagtc agattgacga tattgatttc atgggaaatc tctggcaagg tttctttcca
     5461 tctgccacgt tctgcttttg ggtctacaat cactgcttgt gccccataaa gcactgcata
     5521 atagacgata aggttattcg caaaggattt accaccaccc agcgaaccga caaaggcaga
     5581 cgctaacgca ttggttactg aacccttaac cccttgactg gcaagagcag gtttcagata
     5641 gacattgcgt ccagtatcta agctgtagcc aacataaatc ccctcatttt cacccagcat
     5701 ttgagtagca ccaaaaccta aaccagcgag gaaatcagag gtcacgtatt gaatatagtc
     5761 attcatatat cttttgctgg caggtaaaaa ttcttcatgt aagcccagca tatccccaaa
     5821 aggtcgtacc agtttcacgc tcaaatcatc atagaaatct ttcacttcat tacaacgacg
     5881 tttaagttcg tcaaggtcat ttgctgatac ccttacaaca taggacagct tatacataga
     5941 ttccttgctt tggtctaaat tggtttccag ctcgttcaca ctttccagag cttccgccac
     6001 attagagctg gtttcattat cactttgcca agcgtggtta tccaagtctt tcagttcttt
     6061 ctttttattg cggacagtag atagggcttt acgattcgct acaatttcca cattcattga
     6121 cgtatcaatc ggaaaggtaa attgctgttg ctggtagtag aagatttcag aggacgggaa
     6181 gtccagttcg ccgacaatac tgttgatggt aaagtaagct acataggcgg tttcgtcttc
     6241 ctgctggatt ttcaaatatc gctgtttttc ttccaccaga cagcgagtag gcttaatgag
     6301 gtcataggtt ttaatcagtg tttcatgttc cagctttttc tttgatagag gatactcata
     6361 ctcttcatag gcagtgcctg tctgtccgta aaggtgttca atcagatagc cgaagtcatc
     6421 cttatctaat cttcggattt tgaaacgacg ggagatttta ttttctaaaa gcttttccat
     6481 cttctgaaaa cgcaggattt catcattact catactaaca aaatcgccca tcagcttatg
     6541 gttcacatca tagacaaaat cagtaaaagc attttttgct tcaacggtaa gacttttcat
     6601 agaaaactcc tgatcgttga gaagcaactt aaaaccgata aagaaacggt agtccacttg
     6661 attttcgcca atcatggata ttaaagcgtc tgtctgttgg tcgattttgt cataggcaac
     6721 cgttttgagc tttccagtga cttcattttt ggaacgttct tgtgcagaaa gtatgctgga
     6781 ttctgtactg atttgtaaag catgaatctt gccatcacga ttttgtgcga taagctgtct
     6841 gaaagaatca tgtacttgta ttttctgttc cggacttaga aatgagtaat tgtagggaac
     6901 aagctcatag taagcgtaac attccccatc tttattccag accagattgt tttcaatata
     6961 tttaattgga tatgccataa aattcactcc taactgctgt aatggcttct tgtggctggt
     7021 ttctgccaag cgttactttt tttcctgcat aggtcagctt tcgtcgcagt gcataagcaa
     7081 tgacagactt caaaaatcca taaggctttt taccatcaaa ggtttttgta gacataaacc
     7141 atgtgaaagc cacaggaatc ccaaagtatt tgagaaatgc tccctctatc atagaaagag
     7201 ggggcaagtt gccaagtatc ataactgcaa agagtgacac gacaaaccat gtcatttgcg
     7261 taaaggttat gggaaacgga agtctaaagt cattgatgga gtacaacact ttctcaacag
     7321 accagatact ggtatagctt cgtattttct tcatgtaatc aatcctttca taaaaatggg
     7381 gatagtcttt tgactacccc gtaaacaaga aaatctgcca gtaagaatgt accgacagat
     7441 ttaatagacg atttcaaaaa tcccgtgatt ggttgagata aacgttcccg aaaggtctaa
     7501 atcccgacca taggattcat aatcaatata gttttgaaga ctggctggca cttcgcctaa
     7561 agcacccgtt tcttcaatgt agtagcgtgc cacgtcagac atatcatcac aatcggaatg
     7621 aatgataata tcttcttgat gttcgcttag ttcttcaata cttgaaaaat gagtgagcag
     7681 agcagatagc tccgattgta gttcttctgg caactccgat accatttccc ataggcgatt
     7741 gagttcgcta atagaagtgt attcgtcaac cgtaaagggt aactcgtaat catgaatggc
     7801 gtattcctca tattcatcat tcaagccgat tttctctttg acttcctcaa agtcaatggg
     7861 aaaggtaaac cacgcaccga ccaattcgcc ctcattgtat ttgcctaaat tcgcaatata
     7921 gacttgcata tcgtccatat attcacgtcc tttctttgta gagattcaaa aatccctacc
     7981 gcacttcgtt tggtgtacca ttcctttgcg gaacataaga aaaccactta tattccacaa
     8041 aagaacggtt ttatttaagc accaataatg cgattgaata gctctagtaa aatgtctttt
     8101 actccagcag cgttgaagac taagccaacc gcaataatcg caataattaa aaagccaatc
     8161 agtttgctaa actcacgctt gaagccaaga tacaagccaa tcacaacgat tgctaaaagc
     8221 accagtgatt gagcgtttga tagaaaccag ttatatatgg ataggtaagt tcctgatatt
     8281 ataaatatct caaacttttc cccccattca cacggagcgt gcgactttca ccgcactccg
     8341 cgttccatcg tgtttatatt gaaaagtatt tatgtagtag acctatatca agtttaattc
     8401 ttgattgcca gctaatactc tatatttatt tagtcttatg agctggactt tatttagctt
     8461 caatagagct atatattttt gtattgtttc ttctgtttta gcgtgtatta gacggtgtac
     8521 cgcctctttt accaaaataa gatttttata atcgtcattt ccaccagctc cagttggtac
     8581 aatatgatga caatgtattt cattagcttg ttgaaattct tccagagtta tagcacattt
     8641 tccatgttga gcagaaaata gcgaaattcg attgtctgcg tattctattg tgcgattttc
     8701 caaagataat ttcattaact ggtacatcag taatttatca acacttagat tggtgtggat
     8761 aaattttctt ccctctagag tgtagcaatt cgctaaatac acctttgcca ttggttttct
     8821 gtgttggata tatccaacag ggaaaatagg ttcgccctcg ccagcaacat accgaagcat
     8881 tttagattta ccatatcttg ttgcctcctt tgtcgtaagt tgtctgcctg tttttctcaa
     8941 tctgcccctt ttgtttgtac ctaatctgtt agtgaagata gccattacag ctctattcag
     9001 tttcatacaa tccagattta cattcgttgc aatctgataa taattttgca tacctaatac
     9061 cattgaatta taaagtcttg tttcttctaa ttccgttttc ccttttgagg gagaagcaat
     9121 tttctttgcc tgtgatacca gcttgtcttt ttggtttttg aattgtttat cacttatatg
     9181 tgatttaaca actaacttct ttcttctcgg tatcatttta atcttgaaac caagaaagtc
     9241 ggagtatctt tttctaacat ttacaattct tgttttttct tgtgatactt ccaattttaa
     9301 tcgttgttca atccattgag tgattgctat tttggctttt tcagcacttt ccttgtagcg
     9361 gcagaaaact ctaaaatcat ctgcataacg aaccatatac atttccttta actttgtttt
     9421 cttcatttct ttgtaagcgt tgcttttaca tgggctaccg cttttattga tatgaaccac
     9481 atagtttttt gtaacaggat tttcctgcca ttggctttct acccaatggt ctaactcatt
     9541 aagaacaatg tttgctagta gtggggatat tattccccct tgcggagttc ctttctctgg
     9601 atatgttatt gtaccgttag tcattttgat tggagctttt aatatccttt ttaaaatgaa
     9661 tatcaagtgc ttgtcccgta ttcccatagc ccatatctgt tttatcagct tggaatggtt
     9721 cacgttgtca aagaaaccct taatatcaaa ttcaatcaca taatgtagct ggctgatttg
     9781 taggcggtta taggttgctt ttattgcatt ttcaactgac ctattaggtc taaaaccata
     9841 gctgttttca ctaaatttag cttcgcagat tggttctaat acttgtttta tacattgttg
     9901 tataagcctg tcccacatac aaggaatacc tagaggtctg gttttaccat ttggttttgg
     9961 tatttctttt cgcctcactg gcttaggtcg atacccatgt ggactgcctg caacaatgta
    10021 tcttactttt ttgacaactg tttctgctgg caatttgcca atatctttaa ttattgtacc
    10081 gtctgttcct gctgttttac tgcctgcatt tgctttgata tttcgatatg ccagtaatat
    10141 attttctctc gataggataa tatccataag atgagtgaaa atatctccat tcttactttt
    10201 ggcatataaa tcatcaaagg tgttttgcat ttcgtaatat tcagcgtgtc ttaaatcttc
    10261 tacacacaat gtttcatttt tatttttagg cataaggctt cactccttta cttgaaattg
    10321 agtgagcttt tttatcttac tcgtaatcct tattgaaaca acataatact tttctaatat
    10381 atacacgact tgagggcatt cctccattct cattacaaga atttcttagg ttcgccctct
    10441 gcttttcagc attagttcaa ctgcttattg ttcttcgtca gtttattctc ttgatgagaa
    10501 cccaatacct ttcctcgttc cgataatctt atctatacat atatatcctt aggtcactag
    10561 ctataagcct gctggcttga taccgcctgt aacgttatat ggcatttcat aacctatatt
    10621 cttactttgc cacaccagct acgatttctc gtacctttgc atttctacaa agttcaaatt
    10681 tagacccgta cattcgctgt ttcgtcagtt ccttatagaa cattctaacc atagatattt
    10741 tatagacccc cgacctgttg gatacgctac ccacctctgg atagttttcg atagaagata
    10801 tatgttcatc aactattacg gtatctttca gccgtcttta ccgagcttca aacattctga
    10861 gtttcctcat acaatgcttg tcggaggctc agggcaggcg tttcagagcg ttacttcatc
    10921 attccacctg tcagattatc agttctccta ataaccaaac tttttcgttc ttttattagt
    10981 gccttagctt ttcactaagg aacgagtcgc acaaaggttt tgtccaaaat tcataaaaat
    11041 gttctcctct ctatattcaa tgaatttgta tttgagttat ttttttgttg ttatcacgtc
    11101 ctgttctttt actgactgtt gcttcaaaat ctgcttgtgt cggtctgtca gtttcgcatg
    11161 gtcgagaatg tcttttacaa cctgcgtctg gttgatttca tcaagtttaa tcgcaacctt
    11221 taaggtcggg gcaacttgat gagatagcca gttcagcgtc ctttggaagg agtaaggctc
    11281 tggttttgtg gttagtttta atcgttcacg attgttccca ataaaccaag cccattcttc
    11341 attcagtttc caatcagaac gaggtttgga atcgtcttta tctacaaaac ggatataccg
    11401 attgataatt ttaaaggcgg tatgctctgg attgtcatag acgagtaaat cacggactgc
    11461 ataataggca cgctcatttt tcaatcgaat ctcaaaacgg ttttttactt ctgcgtcttc
    11521 aatgggaata tcatttttct tgtactgctc gtagtccttt tcatagatac agaaataaac
    11581 ttcactttgt aatgaaccga tatagagggt gtttcccata cattcctttt cctctttgcg
    11641 taccagttcg ccactgcgat agcttttaaa actgcggaag acggagatac attcttcctg
    11701 ttggcacttt tcagtgagta cagggatatt taaaatccct gtcttatcgt taatggcaag
    11761 gtcaaggcgt ttcatcacac cgccagccac caaaacgtcc ataaagaact cataccagct
    11821 tctttgttgt gccagaagat agctttcaaa ttgtctgcac ccacgacctt tcaattccac
    11881 cagaactcct ttgtccagtt catgggagca aaggacgaat atgtcgccta aagcataatg
    11941 ctctgaataa gaatagaaac catagtcctc atgaagaaaa taggacagtt tcagttgtaa
    12001 gatgttttcg accacctgct gtacgtctgt tgtcggaaag cgaattctta cataatcaaa
    12061 cagcatttca aggggagcgt cgggattgaa gcgttccaga gcttcccaaa gggactgctg
    12121 taaatcctct gatggcttga cttttcctgt ttcaatatcg cttagatact gccttgtaat
    12181 accagtcgca acagctaaac ggttttgaga tagtccataa gccaagcgtt tttcttttaa
    12241 atgctgtaac caagtttgtt cattcagtaa aaatccctcc aatcaaaaag gcgtatgtca
    12301 acttttaaag cccatttgac atacgctgaa attttgtaaa tcccttgtaa ccaaaggatt
    12361 ttctaatgtt tttttgactg tttcctgtcg atttgtaccc ccctgttaga taccgagggg
    12421 ttaagtgctg gcgtggctat tgccacacca gccagcaaga tcagtccaca cctgcgactt
    12481 ccgcttcgca cgtcgcctgc gtggactgtc tgctgttgga taacttttta atttcctcca
    12541 agaaatcata tccttttggt acaaggggag tataaaactc tgatatgaca cttgttccta
    12601 catcaacata gccacgacct ttgattcgct ttaagaagaa atccttttgt acgtcactgc
    12661 caaacatcat gccatagccc atttcagaca tacgacctaa agccactctg aaattaaact
    12721 gatcacggat tccgtcgcct aaatattttg cgtctggacg ttgacaagcc agtattagaa
    12781 agaagccagc ttgacgacct aacatgacaa tctgtttcag cttattcata actgcggtgt
    12841 tttcttttgt tcccagcatt tccatgaaag cgacgtattc atcaaagatt aagaagtgtg
    12901 ccgggagacc taagtaagca taatttttgc cagtcttata gttcttcatc tgcttcattt
    12961 cctcactacg tttcatcatt tcttcataga atgtttcaat gcaagaaagc aagtcttctt
    13021 ttctatagta gacatttgcc atcacagaac ctaagtccgc aaggtcagca tttttcgggt
    13081 caagaatata cagttttgaa tctgtatgaa gcaaggcttc aatcagtgtc agtataaagt
    13141 aagttttacc gccacctgta ccaccagcaa tcaacatatg agggagctta tcatattccc
    13201 accatacgtt tttcattaag cgaagtttac catctttagc ttctacttca tcaatagaaa
    13261 tacgactggc tatggtgtca tagagcaaag tatattccac ataggaatcc tttaactctt
    13321 tatccgtcag ctcacagtac aagccactct ctaatttctt ttccaagtgt aagagttggt
    13381 cttgatattt tcccagcgtg atttccaccc gtatctgtat caagccattt ttaagtcgat
    13441 aatacatttt agggaagtag gttatctttt cctttgtacg accagcacta tctttaaaga
    13501 aaccctctgt tttgacctgt tcagattcat accacttgtt ttcaagtatc atctttgcca
    13561 gtttttgacg gtggtaaagt tgtttaaccg tatcatagcg aacccgtttg aatacaaacg
    13621 ctaccagcaa gcagataaga attgcgacac tgaaactgat aattaaatag ggaatgtcaa
    13681 tcttatctgc ttgtgatagg ttaaaatcct gccagttgat ctgctggatt gtcttcacat
    13741 gaaacagtcc gacaaccagc aggaaaacag gcaggagtga cgctatcgta aaatgaaaga
    13801 ctaaatcttt accagatggg cgaatccttt taccacgctg tttcatgcga aaaagtctcc
    13861 tttctaccta gcgactattt gtcttgtgtc ggttctttct ttgcttgtgg ttgagctttg
    13921 aatgaactag aatcctttgt cagcacaata tcgtctgcct tgatatacca gtcaacatct
    13981 gctccttgat aggtggcagt agcaacggtg tccgcaatgg gattgataag ttccacccgt
    14041 gcgttataat caaactcttt caaaggcacg ctggcaggaa tacttacttg aatcatgcgt
    14101 ccttgtcctt tggattttaa gtcataggta cgttccttga tttcatctga aaccgacccg
    14161 tcttcatttt ggattctcac ttcacgacgt agagcagaga atttcaattc tccaaaagtc
    14221 gtgtctttat ctaatacaat gccatttgct aatctcatca tttttcctct ctttctttat
    14281 tcttttatca tgtcgtcagc atgtaaaagg taatttgtaa aaccacgagt gccgattttg
    14341 tagccctctg cggtaatacg tggattgact aacttcacac gttcctcaaa gccgaaatgt
    14401 ttttcgccag cttcagcagg aagcaccacc acaatatcat ctgctctttg aacatcagaa
    14461 tagagattat agcttcttga taagacagtt agccgtccgt tgattcttcg ctgaacgact
    14521 ttatcctcgc cagcaaattc taaattgccg aatgtttttt ccatgttggg aatcacaaat
    14581 ttaagttcca tatttttacc tatcctttct tttttattgg ctgaatgaat gtttgatggt
    14641 cttaaagagt ggggaacgac cttttgattc ttgatttttt gttttcataa gttcacttcc
    14701 tttcaaaatc gggtaaaaaa atagacacct cattttttga agtgtctacc tattaaatat
    14761 tcaaatttta ttggaagtat ctttatatct tcacttttca aggataaatc gtcgtatcaa
    14821 agctcattca taagtagtaa attagtagta aattgagtgg ttttgacctt gataaagtgt
    14881 gataagtcca gtttttatgc ggataactag atttttatgc tatttttctc ttaaaaaata
    14941 tcattatttg tggtatggag cccccgcgtt gatccggaag gcacggtaga tttgctcgac
    15001 gaggatcagg cgcatcaatt gatgtggata cgtcattttg ccaaaagaga tctgagcatt
    15061 gcttcttttc atgacggctt cgctgagtcc taaagaaccg ccgataacaa aaacaaggtg
    15121 gctttttcct tggataccta attggtcgat ttgtttagca aaagattcgc ttgtgggatt
    15181 tttcccttca attgctaagc catagacgta ttcttgttct ttgattttcg ctaagatccg
    15241 ttctccttct ttttctttta cttgtctcat ttgcgcttcg cttaaattct ccggggcttt
    15301 ttcgtcagga acttcgatca attcgatttt cgcataggca ttcagccgtt tgacatactc
    15361 attgatacct tggatcaagt atttttcttt taattttcct actgaaataa ttttgatatt
    15421 catgttttct ctcctttgat cttcacgctc actttgttta ttataatcaa ttttttcgtc
    15481 tatatgttta ttttgactat ttttgttaaa aagatttccc caacagttat gcacaacatc
    15541 cacaggttta tccacagaaa acagcgttgt tttaccttat ttcatcacat tcattaggca
    15601 aacatacgtt ttataagaat ttatccactt tatacacagg ctgtggatat gttttatccc
    15661 ttatttttcc aatcctatcc gcgtagtttt acacaatatc tgtggaaaaa gaaaatcaaa
    15721 tattttcatc tgaaaacaat gatttttcct aaagtttttc ataaaaagtt caaagtctca
    15781 aagtagacta aagtcaacaa ataagactat actaacagta gataaatgaa tgtaattgga
    15841 ggaggaacac atggatagaa aaaatgtgac accaaagatg aaaaaaaata aaaataatag
    15901 cctctggcgt aagttgggtc tggggcttgt tggagggatc gttggaggac tactgacagc
    15961 tggcattttt tatgcagcga tgggcactgg caacaatagt tctactgcta caagcggcaa
    16021 tcaaaattct gccggtgaaa cagttgtgga aaatgtcaaa gtcaatgtag attctgatat
    16081 caccaaagcg gtagataaag ttcaagacgc tgtcgtttct gttatcaact tacaaagtca
    16141 aaaccaatca agcggttttg gcggattatt cgggcagcaa gaagaaagta gcagctcaga
    16201 tgatagtaat ttggaagctt atagtgaagg aagcggcgta atctacaaaa aagacgggaa
    16261 tactgcttat gtagtcacaa acaatcacgt agtagatgga cagcaaggtt tggaagtcat
    16321 gatgaaagac ggtacgaagg taaaagctga attagttgga acagatgcct attcagattt
    16381 agcggtgttg aagatcaatt cagacaaagt cgaaacggtt gcctcttttg gggactcaag
    16441 tgctttgaag gttggtgaac ctgcgattgc gattggttcc ccattaggat ctgaatatgc
    16501 gaattctgtc acttcaggaa tcatttcttc cttgaaccga caagtaacaa gtacgaatga
    16561 atcgaacgaa acagtcaata tcaatgcgat ccaaacagat gcagcgatca acccgggaaa
    16621 ttctggtggt cctttagtga atatcgaagg acaagttatc ggtatcaact caagtaagat
    16681 cgcaagtact tctgaatcga cttctaatgt aagcgtagaa ggaatgggat ttgccatccc
    16741 aagtaacgat gtagtgaata tcatcaacca actggaaaaa gacggaaaag tgacacgtcc
    16801 agctctaggt atcacaatgg tcgacttatc tgccgtttct actcagcaac aagaacaaat
    16861 cttgaagatt ccttcatctg taactaacgg tgtcatcgtg acaaatgtcc aaacagctac
    16921 accggcagaa aaagctgggt tgaaacaata cgacgtcatt actaagatcg atgacaaaga
    16981 agtttcttct ggtgtagaac ttcaatctgc tttgtataag aaaaaagtgg gagattctgt
    17041 caaaatcact tactatgaag gctcgaaaga aaaaacaact actgttgaat tgacgatcga
    17101 tcaatcggca ttaaaacaaa atcaaaatag tgaaaattaa taaaaaagag gttgtgacaa
    17161 agacttgtca caacctcttt attttggaaa tacttctccg actacggcat cctcaggcaa
    17221 tactaagatt cctttttttt ctggagcatt tggcaaatgc aattcacgag cagaacaaat
    17281 cattccatag ctggctaccc ctcgcaaagt tcctggccag atcatcattc catctggcat
    17341 catggctcct ggttttgcaa cgacaacttt ttgtccagct tcgatatttg gtgcaccgca
    17401 aacaatttgc aatactgtcc cattgtccac ttctgtctca gtaatcgata aatggtcaga
    17461 gtcaggatgc gggacgcatg tttttacata cccgacaacg atcttcggtt catgatcagc
    17521 ttcaagctgt tcttcgaatc ctgcttcttt aatcaatcga ttcaatttcg ctaattcttc
    17581 atctgttaag cttacttggc cgttcccttc aattgttaga tgatcggaga tatggaaaaa
    17641 gttccaagca acaacttgac cttgttcatc tttgatccga gcaacattcc atttacgttc
    17701 gacagtgttt tctgttcctt tatcgtcggc tatgataacc atcaaagtgt ctccgacgtg
    17761 tgctttgtta taagcaaaaa tcatgttctt tcctccatta tcctttgctg agatttatct
    17821 taacaaaaaa ttgcattcac tgcatgagat ttttttcatg ctttatttcc gaagaagctt
    17881 cttctggaac accatttatt cgtaacaagg gactgtgaca agacttttgt cacagtccct
    17941 ttactgtttg attaaaaagg ttatacaagt tccacttgct taattactcg gctgttcttc
    18001 ttcgtcagaa acgatcaaga catcacaagg tgaatgacgt atgacgtagc tggctacact
    18061 tcctgtcata aagcgttcga ctgcatttag tccagactgc ccaaccatga ttagatcgat
    18121 tgcatatttt tttggcaatt tgactgccat ggattcacga acagagccgt atgcaataat
    18181 tccttctata tttgtgtaat caacagattt tccatattct ttacattctt cgatcatttg
    18241 cttggctgtt tcagtttgct gatcgactag attttgatca ataggagcat agcccataaa
    18301 attataaaat tgctgatcga ttacgtttgc tacatatact ttcccattat tacggcgagc
    18361 cacttctaac gcttttttga aagcagcaag tgcttgttca ctaccgtcaa tacctactaa
    18421 aatgttctta tattcttgat tcatcttgac ccctcctaac agatgtcagc cttttattac
    18481 actttctagg aacgattcga tttcctgttt tgttttacga tctttattca ctagacgacc
    18541 taattctttt ccgtcttgga tcacgacaaa acttgggata ccaaaaatgt tccattcagc
    18601 ggctacatct atgtacttgt cacggtctac ttcaataaat tgccaatcag aaaagtcatt
    18661 ttcgatttca ggcatttgcg gtttgataaa acggcagtct ccgcaccagc cggcagtaaa
    18721 gaaaaataca ctttttccag tttctacgta gcctgctaat tcttctaaac tatttgggat
    18781 aatcattctt tttcctctac tttctgttta tgtatttttc aattaaatta tacacctttc
    18841 tttcgaacta acaaacattg atcctctttt tataaaaaaa atctgacaaa aggttgcttt
    18901 tgccagattt tatagtttca ctatcgcgtt atttcccgta tacgatcgtg ttcactgttg
    18961 atttatctaa acctttcaat gtttgaatca acatttctcg tgcagcttca aaatcgcgaa
    19021 tactgaacat cgtttgatgc gtatggatat aacgtcccac tacgccaatc actgtacttg
    19081 ggattccttc attttgtgta tgagcagcac ctgcatctgt accgcctttt gaaacaaagt
    19141 attgataagg gatgttgttt gtttcagcga tatctagcaa gtactcacgc aaacgaggaa
    19201 gcatgatcaa acctggatct tggatacgca tcaatgtacc ttctcctaaa tgtccgaatg
    19261 tgccattttt cgtcactgtg tcatctgctg cagagcagtc tactgcaaag aacagatctg
    19321 gcttgaattt attgacagaa gctttagaac cacgcaagcc tacttcttct tggacattcg
    19381 cgcctgcaat caatgtatga cctaatgttt cattttgtaa cgcttctaaa gcttctaata
    19441 ccattgtaca gccatagcgg ttgtcccacg atttgctgat gatattcttc ccgttggctg
    19501 ttttgatcgt ttcagcgtaa ggaacgattg tgtctcctgg aagaacaccg taactcattg
    19561 cctcttctct tgattcaaac ccagcatcga acaaaatatc tgttacttct actgattttt
    19621 gtccgcttgt tcctcgtaat aaatgtgggg gaacggaaga agaaatacat ggataattct
    19681 taccagtaga ggttttaagt gtaaaacgtt gagcagaaac aacgtaaggg ttccatccgc
    19741 ctagaggtac gactttaaaa agcccgtttt cttggatttg tgtcagcatg aatccgactt
    19801 catccatatg tgctgcgacc attacacgag gtgcatccgc ttcttcatgg tggcgcagcc
    19861 caaaaatgcc gcccaaacca tctaactgta actcatctac cagcggttcc atatgacttt
    19921 ccatataggc gcgaacgtct tgttcaaaac cgcttgttcc ttgtaattct gttaactctt
    19981 tgattcgttg aaatgttttt tcttccatat gctattcgtc taaatttcag actaaactcc
    20041 tctcagactt atattgatct gcggataaaa tatgattttt tctgcagacc gatcgtattt
    20101 attatagctt attttttctg gtagcggtta tttatttcaa agaaaatatg agagtgtggt
    20161 aaaataaaca caaagagtag caagtgagaa gaaggaggta tatccacatg tatgaagaaa
    20221 gcgaattaag ctattttaaa ggcgggttag ccgttgggat cggtctagga ttattgagtg
    20281 gggtagcttc aacgatttgg tatcatcgca aaaaagtgtt atcagcagac caagtgcttg
    20341 aaaatatcaa aacatctttt ttaaaagaag gcgcaatcga aggtgcttgg atctcttttg
    20401 aaaaagaacc tattcggaaa ttcgccgttc ggtcgagtgc ttatcggggt gggctttccc
    20461 ggatagaaga tggcgaaact gtttattatg aatttttagc agatgctcat acaggaaccg
    20521 tattagacat ttcaagaaaa aagggcgcaa tagattaata cgtcaaaaaa atgaaataac
    20581 gaatacgcac ccactatcta aaagagaggt atcctatcct tcttttatag tgggtgattt
    20641 ttataaaaat agggctattt cctcttggaa tagctagtat ttgtagttat tatacggtat
    20701 tcttgaaata agattagttt tattatataa ggagagtgac aaatggaatc acgtgaaaaa
    20761 attgccgtgc tcattccttg ttataacgaa gaggcgacaa tttcaactgt tattgaagat
    20821 tttaaaaggg aattaccaga agcagatatt tatgtctatg ataataattc gaccgatcga
    20881 acatacgaat tggccgttca aggcggagca atcgtaaaga aagaaccacg tcaaggaaaa
    20941 gggaatgtaa tccgtcaaat gttttttgat attgatgccg attattattt gatggtagat
    21001 ggtgatgata cttatcctgc agaagctgca catggtttgc ttgagaaatt acgttctggc
    21061 gaagcagata tggtcattgg cgatcgtcta tcaaatggaa cctattttga tgaaaataag
    21121 cgtcctttcc atgattttgg gaataatctg gttaaaaata caattactta cttatataaa
    21181 acaaaaattc gcgatgtcat gaccggctat cgaggattca atcgaatttt tgtcaaaagc
    21241 ttccctatta tgagttctgg ttttcaaatc gaaacagaat tgacgattca tgctttagat
    21301 aaaaaattca agttcgttga gattccaatc gattatcgcg atcgtcctga aggtagcgaa
    21361 tcaaaattga atacattctc tgacggattc aaagtgatca tgatgatcat caaaatgtgc
    21421 aaagattata aacctttgct tttctttggg atctggacca tcttcttctt tttatttgga
    21481 ttggtcatgg ggattcctgt gattcgtgaa tatatgctga caagctttat tacacgcatc
    21541 ccttctgcga ttttaagtac tggattgatg actttagccc ttctttcatt agtaacaggg
    21601 ttgattttgg atacggtcgt cacaaatgcc aagaaagaat atgaattgaa tttataccat
    21661 gtctaccacg aatatcaaaa taaagaaatg aagtaggagg ccttttttat gcataagaaa
    21721 aaggagaatc tatttcctac tttttttgca actttatttg cgtcaggttt tgcttttttt
    21781 acttcttatg gtctaggaac tttgaatcca acagtaatca ttggtttagc catggttttg
    21841 ctacttgctt ttaagcaata tggaatagag ttgttttcaa taaaaaacaa agttgcagct
    21901 attgcccgat tgtttttttc tgcagctttt gctcttacac tttctattgg cagacagata
    21961 caatttcagt atgtctcagc aaactataca aagacaacga ttgctgatgg aagtattttt
    22021 tactttcttg tttatttctc catttttgtg tattcttttt tatttttaca atcgatttat
    22081 ttatcaatgc caaaactgga aacatttttt actgctcaaa acacacaggc tttctcttca
    22141 agaaaatatt ttttattctt tactgtcttt ttagttctgt gttggcttcc ttatctgttg
    22201 gcattgtatc ctggagtggt tttgcctgat tcgttaagtt cggtcgccca atctatgggt
    22261 gatgttccta taagcaatca tcatccagtc ttattcacct tactggtcaa gctatttgtt
    22321 catgatttac caactgatac gatcaatcac ggtgtatttt tattttcgat ttttcaaaca
    22381 ttgataacag cttcagcgat aagttattct ctttgctggc tgttaaaaag aaacatccat
    22441 ttttttccgg tactcattag tttatgctat tttgcatttg caccggtttt tccaatctac
    22501 gccttgaaca tgcaaaaaga tactttgttc tctatcgctt gctttttatt ttccttagcg
    22561 gtttttgact ttatttttca acgagaaaag ccaacgattc tcaatcagat ttacgtaatc
    22621 atccttgctt tactgaccac ctatctaaga aataatggga tttatgtagt aaccggcaca
    22681 ggtttgattt gcggcttgat tattttgaaa caaaaaaggt atcaagcatt ctttcaagtg
    22741 gtgctgggtt atacagtgat tgctggacta ctcatccacc cgttgatggc tcgttatgct
    22801 attcctacag aaactgctga acaattaggt gttcctttgc agcaaatttc tagaactatt
    22861 gttatggatg gtgacttgtc taagaaagat aaagcattca tgaatcagct actcccgata
    22921 aaagattatg aagcgtattc tcctagtctt gcagactcaa tcaagtggca taaaaatttt
    22981 aacaatgagt ttttgaatac acataaaaaa gaatttctat cactatgggc aagaaattta
    23041 ccaagtaata taaaaatcta tacagatgct tatattttag aaacgttcgg gttctgggta
    23101 cctggtacaa aaaatagtta cggcttttta gatactcgtg taaacacgaa taattatggt
    23161 atgaagcaag tagatttgat tgaacatctt actggattgt caggaatgaa aaaaatcatc
    23221 gatgctcgtg atttctttgg ttccggcaca ttactgtggt tagctatcgg cagtgcactt
    23281 ttagcaatct ttaagaaaaa atatcggtat ttactccttt ttcttcccgg tatgcttgtt
    23341 tttgctacta ttatgattgc tactcctgtg gcttttagtt tacggtatgt gtttgtttta
    23401 gcattaggtc tacctgttta tatcctctct ccttttttac tagataaaag agaaaaaatg
    23461 aaaacagata taggagatac tcatgaaact atttaaaaag atattttttg cttttgttct
    23521 cttgatcgct agctattgcg tactactgac aattactttt ttcattccgc agcactctat
    23581 tgaatcgaat gcagaaaaat ctctacagat ggttgaaaaa gaaggaatgt atccttctat
    23641 caatcaagga aatatgctag gcactcggtt agataatttt actgatcatt tgatgatacg
    23701 aaaaacaaaa gcagatcctg aattgaactc cttggagaac gcgatgtcta tggcggatta
    23761 tccccgttat tggcatggtt atcagctttt tttacgacct ttactgctag ggatgagttt
    23821 ggggagtatt cgaatgattt acgcagccat tctttttctg ctgattggat tgacgagcta
    23881 ctttttaacc aagtgtagcg acatctattt aacgacagcc ttgttgattt cgcttgctat
    23941 aggaaatgct gcaacctttt tcttcagtat gcaattttca aatatttgga ttttgacttt
    24001 attgtctgta ttactatttt taacaaaacc acaactctcc aacaccaacc aacaattatt
    24061 cttgtatttc tttgtcattg gttctttagc taactttttt gacttgttga caacaccggt
    24121 catctcttgg gggatacctg tgatcattat ttattatgta acaaataagt atttaatgga
    24181 taaacgcagt tcattgagca aacaagtagg cagttttgtt tttaccggta tcttttgggg
    24241 attaggctat ggtttgacat gggtggctaa atggatccta tcatcgatca tattgaacaa
    24301 aaacattgta aaagatgcaa tcaaccaaat cctctttcga acggaaggaa acgacaaata
    24361 cccgttgcat cgattagaga tgctgaaaag caatattaga ttgatgtatc caaaagtagc
    24421 aatattattg ctattgatta cttgtcttgt ttttctctat tttgcttatc gtaaacgaaa
    24481 tagctggcgt ccgttccaac ttctagtttt gtttgtttta gctgctgtaa cgccgtatat
    24541 ttggtacaat attttagcta atcactcaca gattcattat tggtttacgt atcgtacgca
    24601 aatcatcaca agttttgcta tacttagtgc tttcgccttt ttgatccctc ctgagaaatc
    24661 aaaagacgaa ctaaattact tttaagaata ccgatgtact aattcatttt taaacataaa
    24721 aactccagct gtcagagata gccggag
//
LOCUS       DAMEET010000038        24587 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.038, whole genome shotgun sequence.
ACCESSION   DAMEET010000038 DAMEET010000000
VERSION     DAMEET010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 24587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.038"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(118..663)
                     /locus_tag="PB780_001372"
     CDS             complement(118..663)
                     /locus_tag="PB780_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDE0733434.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(675..1367)
                     /locus_tag="PB780_001373"
     CDS             complement(675..1367)
                     /locus_tag="PB780_001373"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDE0733435.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1386..1670)
                     /locus_tag="PB780_001374"
     CDS             complement(1386..1670)
                     /locus_tag="PB780_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733436.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1683..2246)
                     /locus_tag="PB780_001375"
     CDS             complement(1683..2246)
                     /locus_tag="PB780_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDE0733437.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2474..3136
                     /locus_tag="PB780_001376"
     CDS             2474..3136
                     /locus_tag="PB780_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDE0733438.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3260..4456
                     /locus_tag="PB780_001377"
     CDS             3260..4456
                     /locus_tag="PB780_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDE0733439.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4557..5933)
                     /locus_tag="PB780_001378"
     CDS             complement(4557..5933)
                     /locus_tag="PB780_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDE0733440.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5970..6836)
                     /locus_tag="PB780_001379"
     CDS             complement(5970..6836)
                     /locus_tag="PB780_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HDE0733441.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6925..7950)
                     /locus_tag="PB780_001380"
     CDS             complement(6925..7950)
                     /locus_tag="PB780_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDE0733442.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7968..8642)
                     /locus_tag="PB780_001381"
     CDS             complement(7968..8642)
                     /locus_tag="PB780_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDE0733443.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIKGYIETKVG
                     QGYIVP"
     gene            complement(8826..9719)
                     /locus_tag="PB780_001382"
     CDS             complement(8826..9719)
                     /locus_tag="PB780_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDE0733444.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9793..11490)
                     /locus_tag="PB780_001383"
     CDS             complement(9793..11490)
                     /locus_tag="PB780_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDE0733445.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11483..12796)
                     /locus_tag="PB780_001384"
     CDS             complement(11483..12796)
                     /locus_tag="PB780_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDE0733446.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12796..16833)
                     /locus_tag="PB780_001385"
     CDS             complement(12796..16833)
                     /locus_tag="PB780_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDE0733447.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FHHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16846..17304)
                     /locus_tag="PB780_001386"
     CDS             complement(16846..17304)
                     /locus_tag="PB780_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDE0733448.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17602..18120)
                     /locus_tag="PB780_001387"
     CDS             complement(17602..18120)
                     /locus_tag="PB780_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDE0733449.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(18122..18739)
                     /locus_tag="PB780_001388"
     CDS             complement(18122..18739)
                     /locus_tag="PB780_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HDE0733450.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18736..19590)
                     /locus_tag="PB780_001389"
     CDS             complement(18736..19590)
                     /locus_tag="PB780_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDE0733451.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(19587..20498)
                     /locus_tag="PB780_001390"
     CDS             complement(19587..20498)
                     /locus_tag="PB780_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDE0733452.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20539..21873)
                     /locus_tag="PB780_001391"
     CDS             complement(20539..21873)
                     /locus_tag="PB780_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDE0733453.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            22081..22911
                     /locus_tag="PB780_001392"
     CDS             22081..22911
                     /locus_tag="PB780_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDE0733454.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(23155..23473)
                     /locus_tag="PB780_001393"
                     /pseudo
     CDS             complement(23155..23473)
                     /locus_tag="PB780_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23595..23789)
                     /locus_tag="PB780_001394"
     CDS             complement(23595..23789)
                     /locus_tag="PB780_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733455.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(24294..24479)
                     /locus_tag="PB780_001395"
     CDS             complement(24294..24479)
                     /locus_tag="PB780_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733456.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
BASE COUNT         7267 a         5323 c         4239 g         7758 t
ORIGIN      
        1 aaaaagaata agttttttta aaataacgta taaaaaagag cataaaacaa agtcgtttgt
       61 tttatgctct tcttttatta ttttgtgctt tttattttat caaatcactt tgttttttta
      121 taaaatttct gcgcctagtg tggactcaaa atgtctcaat gcccattcat gtccaacggg
      181 atcgaaactt gccacggaat ctttgaaaat atggagtttg tatcctagat tatacgcatc
      241 gactgctgta tgcaacacac agatatctgt acatacgccg gtcaaataaa tatctgtgat
      301 ctggcgttct cgcaaacgga tgtctagatc tgttccacta aaagcggaat aatgccgttt
      361 gtctatccaa taaacatttt cctcttgctc atagtcttgg tataaacttt ttaaagagcc
      421 gtacaattcc cgtccacccg ttcctatcac attatgcgga ggaaataagc ggttctccgg
      481 atgaaaggaa tcttttggat catgcgcatc aatggcaaaa acgacaaaat ccttttgatc
      541 aataaacttc tttgtatgag cagtcagctt tttttcaatt acttgtcctg ctgtcccagt
      601 agtcagcttt ccatcctctg caacaaaatc atatgtgtag tcaatcgaga tcaatgcgtt
      661 catcttagtc ctccttatac gagatgtttg acaaattcga tcaccatttc tgctgatcgt
      721 ttgcctgcgt cttcgataaa ttcgtcaaag ctttgtgtcg ccgagtgatc tgctgtgtcg
      781 ctcattgcac gtacgatcaa aaatgggata ttgaactgac gagctgtttg tccaacagcg
      841 gctccttcca tttcgcaagc taatgcttca gggaaattcg ttaagatttc tttgatttta
      901 tctggcgagt cgacaaatgt atcacctgta acgatcaatc cttcttttgc gttcaaattc
      961 gttgcttttg ctgctttaac catttcactg cgcaaatatg tgcttgcttc ataatataga
     1021 ggcattcctg gtaattgtcc tgggttgtag ccaaatcctg ttgcatctgc atcaaaataa
     1081 gctactttgt ctgatataac gatgtcacct acttgtaagc cttcaccgat tccgccagct
     1141 gaacctgtat tgatcaccat gttcacgccg tattgttgga tcaacagact tgtggtaata
     1201 gatgctaaga cttttcctat accggaacgt acgacgatca cttcgtggtt acctaatgaa
     1261 cctgaaataa ataaagcgcc tgcgcgttcc catgataatg gttcgcttag gttctctctc
     1321 aaaattttta cttcttcttc cattgcgccg ataataccga ttttcatctt tttaattcct
     1381 tctttctata taaatgcgat tgctaaaaaa accacacata acattaggat aacaataata
     1441 agagatttca tcaaaaaaga gttccatttg gtcgtctttt cccgttcgct gatccttgtt
     1501 ttcgtgattg gtttattctt tttgctttct ttacgataaa agctggcaat ctttttttct
     1561 tcttgctgat aagctgtttc tgcttttcgc tgttttttta atgactcgct tgcttgtgct
     1621 tgttgtcgct tgcgaagttc gcttcgcgta ataagcggtc ctttggccat aagccgatcc
     1681 ctctactttc ctttggtcaa tagttcccag tattgaatcg cataaatcgt ctttgcatca
     1741 caaatcaact gatctttcat cgcttgttgc gcctcttcaa gagttagatg atacaactcc
     1801 agtacttcgt cttcatcttg tgctaaagga ttctcgacct tctctacccc ttgtgcatga
     1861 taaatatgaa ggacttcatt tgcaaaacct ggtgaaagat acatcgaagt aagatggctc
     1921 agcgacttcg cacgatagcc agtttcttct tctaattctc tagctgctgt catttcggga
     1981 ttttgtcctt ctcctggatc gatttttcca gctggaatct ccaaaatcac tttttctaaa
     2041 ggttttcgaa attgtttgac aagaagcaga cgatcttgtt cgtcaaaagc aatgatcccg
     2101 acaccgccag gatgaaatac cagttctctc ttcgaagttc ctccatctgg caaacgaact
     2161 tcgtctacag caacatcaat aatttttccg ctatagattt ctttgcgact gattgttttt
     2221 tcttcaaatt gctcattgtt cagcatatct tctcctgctt tctttgtaat cataactgct
     2281 tttttttcat ttttcaattt ttctcaaaaa ctccttatta gtttaccata gataaacctt
     2341 agattttctc taaatataga aaatccttct ttagtccgat actctttcta gtagtccgta
     2401 tgctaaaata agtatgtaat cgaataacga aaagaattca atactcaacg ataaatgaca
     2461 tggagggttt attatgataa aacgaaattg gccgtggctt ctagcattgg tcttgctatt
     2521 tctctttgtc cgaaacaata gtttttcttt tgttctagga cttttattga taggtgtagt
     2581 tttagttttg tggggactct ttggttcgcg caggaaaaag ggaaaacaag aagaaattcc
     2641 tgcactgact gacgaacttg aaactcacta tgaaaaaagc ggtatgacag cgaatgaaat
     2701 ttcatttttt cgacaaacaa tgaatcaaac aaaaaatgag atcaatcaat tgcaaatgaa
     2761 tatgcagcag acagccaaac ttaaagcaat cgatctccgc catgatccag tcaaagcagc
     2821 gaaagcttta ttcaaagaat tggtgaaaga accaaaccga ctgcatgaag caagtcagtt
     2881 tctttatacg catctgccga atctagttga tttgacaaat aagtataatg agatcaatga
     2941 ccatgagatc aaaaacaaac agacctatga aaaactagaa gaaagcgcgc agatcatcga
     3001 ccagctgtca gctttaatcg cacaagatta tcaaaaattc gttgctgagg atctagatga
     3061 tatcgacgta gagatatctg ttgcgaaaca gagcttaaag cgtgataata aatatgaacc
     3121 agaacaaaag gattaactct tttgttcata aaagaagact cggaacattc agatgatgtc
     3181 tggctgtttc gagtactttt tctaaagtag agacattcat gtaagaatcg agaacagatt
     3241 caacaaggag gaatttttta tggatcataa accgactgaa acaaaagatg ttacaccagt
     3301 taacgataca cttgaagatt tattgaacaa cccgttttca actccaacag atacgctgac
     3361 cacttcacaa caagcggaga ttgatcagct ccaagagcaa cagacagctg ctcgtttaat
     3421 tgacaaacta ccggcggaac gccaagaaca agctaaacag ctagcagcaa aaatcgatgc
     3481 taatgatgca cagtctgtca tcagctacgg atcggctgcc caagcaaaat taagtgaatt
     3541 ctcacaatcg atgttgaatc atgtgcaagc acaagatatt ggtcctgtag gagattcctt
     3601 aactgaactg atgtatcgtc tccaagaagc caatcctgac gaactgcgtg ccggagaagg
     3661 gaacatcttc cagcgtgtct tcgggaaggt caaacaatcg atctatgaag tgacagcaaa
     3721 atatcaaaag attggtgcac aaattgacaa gatttcagtg aaattagata aagaaaaaga
     3781 cggtctgcta aaagataatc tgatgttaga acagctttac cagaaaaaca aagactactt
     3841 tgatgcactg aatatttata tcgctgctgg agaattgaaa atggaagaac ttcaaacaac
     3901 gatcattcca gaagcgatga aaagagcaga agaaactggc gatcagatgg atgttcagat
     3961 tgccaatgat tacacgcaat tcctagatcg tctggataaa cgtacgcatg atcttcgctt
     4021 agctagacag atcacgatcc agcaagcacc acaaatccgt ttgatccaaa acacaaatca
     4081 ggccttggct gaaaagatcc aagcatctat cgctacagcc attccgctgt ggaaaaacca
     4141 agtcgtgatc gcattgacct tgcttcgtca aaaagatgca gtaactgctc aacgccaagt
     4201 ctctgaaaca acaaacgact tattgaagaa aaattcagaa atgctgaaaa tctcagccat
     4261 cgaaacagcg aaagaaaatg aacgtggaat cgtggatatc gagacccttc aaaccactca
     4321 aaatgatttg atcgaaacaa tccaagagac attgcgtatc caaaaagaag gaaaagaaaa
     4381 acgtcgtcat gcagaagtag agcttggaca catggaagaa gatctgaaac aaaaactgtt
     4441 ggatttaacc caatagatca cctttcgctt gttactaaaa caaacgccca acaacagttt
     4501 attacaactg ttgatgggcg tttttcttat ctttcttcta acttctccat tggagtctat
     4561 ttctcattca tcttgctgtg tccatagcca tagccaaaat aaatgatcag tccaataacc
     4621 attacgatac cgaacacgat ccatgtcaca gtttgcagac gaagcatcaa tacaatacaa
     4681 gtcaagatgg agacgattgg caatagcgga acgaatggca ctttgaattc tccagctttt
     4741 ggtttgccaa acatttttct caacttcagt aagcctagac taacaagcat caaataggcg
     4801 agcgccatga tattcgttag ttccgctaat acattcaatg ggaaaaatcc ggcaaagaat
     4861 acagctgcca caccagcggc ataagtcgct tttttcggtg tacgatgctt ttgatcgatt
     4921 tctgtcataa acttaggaag taagccatcc ttgctgacag caaacagtaa acgagcaagt
     4981 gaatacatca tcgagatcgt taccgtcagt agcgttaaaa ttgcccctac agcgatcacc
     5041 gcgcccactg ttccatgtcc tacatacctc attgcaaaag ccactgggtc attgactcct
     5101 agttcagtat acggaacgat tcctgtcaag atcagcgtca cgatgatgta aaggacagta
     5161 gcaatcagta tcgaaccaat gattcctctt ggcacatctt tttgcgggtt tttgacttcc
     5221 tcggctgcca ttgcaacggc atcaaatccc aagaaagcaa aaaacacaag agctgcacca
     5281 tctagcacac cggaaaaacc aaaaggcatg aatggctgcc agttatcagg tttaacgaaa
     5341 aatattccta caatgataaa caacgcgatg ataccaaatt tgacatatac catggcatta
     5401 ttcaaacgta atgctttctt ggcttcaagc gacacgatgt aagtgacgaa tatcactacg
     5461 agcaccgcaa tcaagtcaat gtatgtacca ttttccggat tgtacccgcc agttaatgct
     5521 tgtggaagtt cggcccctaa acttttcaaa aaaccttgca tatagccaga ccatccggaa
     5581 gcaacagagg aaaccgctag taaaaattcc ccgatcaaca accaccctgt cagccaggca
     5641 gcaaattcac caaatacaac atacagatag gcatatggcc caccaatcac tggcactcgc
     5701 gaagcaaatt ctgcgaaact tagtccagac aaaatgacaa cgattgcagc taagacaaat
     5761 gaaagtgaca gtgctggacc agcattttga ttggccgcaa cccctgtcac cacaaatatt
     5821 cccgtaccga tgatcgcacc aattccaagc atgatcaaat cagacgtttt tagttctttt
     5881 ttcatctcac tcgtatgatt gagattgatc tctttcttac gaaacaaatc cattgaagtt
     5941 cctccccatt ttaggttgga aaacagcgat taaactgttt tcatataccc taaaaggata
     6001 ccacctaaaa tgactaaaag gcaaccaaca ataacataaa tgagctcttt tttcgttttt
     6061 ctttcgccaa ggatgaaaat cccacctaaa gtagagataa tgatccccat ttgtgaaagt
     6121 gagaagccaa cagctaaacc aagtgatttg actgttagaa gcatgcagat gtttcctaag
     6181 ccccataata atccacagat catgtttttc cacacaaact gatcgacttt aactttacgg
     6241 aaagcaaaga agcttgcgcc taagatcatt ccgatacttt gaggtaagat aattgacatc
     6301 gcatccaaat tcgcccagtt gacgatgatg gtatatacac cataaccaat cgtagagaag
     6361 atcaatgctc taaaaccctt accaaaatca agcattttat tatctgtatc tatttttcct
     6421 tcgtcatctt gtcgggcagt caaatacgca ccgcttacaa gaagagctaa agcaataata
     6481 ccgtatacat aatctttcgt ttgtgtccat tcatggaaga aaacggctcc cgcaatcgta
     6541 ttcaaaatta attgaaaacc agtagacaat ggtagcccta cagaaacacc catgtacttc
     6601 attccatgga actgtccatt ttgtccaaca ctccaagcta agccggataa aaatccgatc
     6661 aagaaaatcc aaggggtgat tacaggacta acaacgaaaa agacaactaa agaaaacaga
     6721 aatgcaccga tcgtcattcc tagtgtttgt tgattggcat cgccaccaat tttaccactt
     6781 actaaaccaa tactacccca tgccaacata gggactaatg ctattaaaat ttccataacc
     6841 aatctccttt tatttcacat gataagaaac ttatcagcga aaacgttaac aacacaagca
     6901 ctttttttcg tttctaacga ttttctaacg tttttcatta taaaaattta agattgggaa
     6961 aaataaagtc attgttgtcc cttttccttc agttgattca gccgtcaaat ggatacctaa
     7021 tttttcggcc agattttttg ctaaataaag tcctaatcct gttgaatgtg ttttgctaag
     7081 tcgaccattt ctcccagtaa atcccttatc aaagatccgt ttcaagtctt ccgctgggat
     7141 accgatccct gaatctttaa ctgaaaggct gactccttgc gcttcctttt ctatgctcac
     7201 aatgatgttt ccgtgatccg gcgtatattt gatggcatta ctaagcaatt ggttgaagat
     7261 aaaagcgatc catttagcat cggtcaaaac agtatgatcc tcaccaacga tctcgtagcg
     7321 aatatttttt tggataaaat aatttgcctg actgcgtatc acaggctgaa tgattccttt
     7381 tagtccatat tcttggatga ggtagtcccg cgagaaactg tcgagtcttg cataatacaa
     7441 aacttgttct acgtattcat cgatttttga taattcattt tctagtaatg tatacttttg
     7501 atcgtcaata tcgaactcga tcgaacgaac tagcaattta gaagcggaaa gtggcacttt
     7561 gatttcatgg acccatgagt caatataatc tttttggtct tgttgattac tgattgctga
     7621 ttgcattact tcttggtgtt cacggatcag ctgattgata tattcttcta ccagtttttc
     7681 ttcttctgtc ctcccaccac tcaaataatt ttgcaaaggg gaagtttttt gtatgtcggc
     7741 taattttccc caccatcttc tcttgctcaa gtaaccaccc agcagataaa aaatcagaaa
     7801 gacgccctca atcaaagcta aataagcaat tgtgctcaaa tcaactttga tgtttggcgc
     7861 cagccacatc acaaagatcg tcaatgcaat aaaaaacagc catcccaaga tcaatgtcca
     7921 ctgatctttc aaatatttaa aaaaattcat cttaaccgct catcctttca gggaacgata
     7981 tatccctgtc ctacttttgt ttcgatatat cctttgatac ctgcctgttc gatttttcgg
     8041 cgtaaccgat tgatgttgac tgtcaatgta ttgtcatcta caaatcgttc gtcatcccac
     8101 agtgctcgca gcaatttttc ccgacttaag atctttccat tgtgcttcat caaaataaat
     8161 aatagacgat attcattttt gctcaaatca atgatctctc cgttgatttc catacttccg
     8221 ttgtcaacat tcaacgtaat gccattgtgg ctcatcattt cactagataa ttctgtgtag
     8281 ttgtaagaac ggcgaagtaa tgcattgatt tttgcaatca gtacatcgat ttggaaatgt
     8341 tttgtcacaa aatcatctgc ccccatgttc atagccatga tcatatccat attcgtattc
     8401 ctgctggaga taaaaatgat cggtactttc gatacttcac gtattttttg acaccaataa
     8461 tagccatcca aaacgggaag attaatatct agtaatacaa gctgtggttc ttctctttca
     8521 aagtctgaga acacttgatt gaaatcggtc gttccaaagg tttcaaattg ccatttctca
     8581 agttcctcca tgatcaattg tcgaatgatc tcttcgtctt cgactaccat gatttttgcc
     8641 ataaaaaatc cctccatctt tcttcttgct actacaatat caaagaagcc cttgtgaaac
     8701 aagttggctt acctggaaaa tgtcatttta gcaaacgtta aaaaaaactg gaaatatgcg
     8761 atttaatcgc gtttgtctct tccaagcagc ttaaatttat tatgttattc ttaataattg
     8821 gtctattatc tgttattttt ctttcgttgt ttttgtcttt taaattttcg ataacaccaa
     8881 taaccgaaaa gcgttagcac tagcaatata cctagaagga gtataagtaa tcgataaaga
     8941 tggtattttt ttgtctgttc tatctttgaa gagacttctt ctgtaaaggg aactcgtctt
     9001 cctgtcacta gtaatcggtg agtgttcacg gtgtaaggtg tacaagtgat aagtgtgact
     9061 agatcctgct tttctctaat tgctaatgta tttgtttttg ttggaaggac aacaattttc
     9121 tcaacgactt cataagcgag catccgacca tacacttcaa gatagaatcg gtcatcttgc
     9181 tccatttctt caagatccgt aaagagtttt ttctcagcaa taccgctatg ggcggtaatc
     9241 acggaatgtg tactctctcc tccagttgga aaagaggtgc cttgcaggac tgtcgctcct
     9301 ctatctaaca aaagatcatt cgtttcatca aataaaggca agctaacatt gattttcgga
     9361 atatagatgg ctccgatcat atgttgttcg taatattctt ttcctggatt ttggacattt
     9421 ctaaccgctt gctcaaaagg atctttgact tgtcccattc ctgggattac tgatgtatga
     9481 gcaagcgtct gatttttctt ttcatgcact gctaattgtt gctcatgttt ttcttcttta
     9541 tcgcgttcaa gctgcttttg ataattctcg atccttcttt gatccaaata attgctgaga
     9601 gcatctgcta aaaatggata tgtaaaaact gcagctccgg caagaaacat cactgccata
     9661 aataattgta ataaaagact gatttgggtt tgaggtctat tacctatttt ctttttcatt
     9721 ttcttcacct tcttatttaa aaaaactgca gcaaccttta taaaaccgat tgctgcagct
     9781 tatttttgtt gcttatactt cagacttcat tttagattta cggtaccagc tgtacgctcc
     9841 aatcatcaaa cttattccga ttagtaagaa tgccaagagc ccgttcccac cagtagcagg
     9901 aagtgacccg cgtttcatat tcggtactct ttctggccct ggattttcta ctcctacgat
     9961 ctgaatttct tcattatagc ttccataaga gatgataaat tcagtaaagg ctgcctcacc
    10021 tgttggaagt acataacctt ctggcgccat ggtctctttc atttgatagg ttccataatc
    10081 taacccttga acctcaaagt atccatttcc atctgcgaca aattctgttg cttgtccgct
    10141 tgttgcccac tcaattctat ctgctcctga ttcataaaca gctaaaggcg actccccttt
    10201 ataaaatatt gcgtattcag aacggtcttc attccacaat tcgaatcttg catctttaag
    10261 caattcactg cttgatgcat catatttttc aaactttctt ccacctgttg tgactttctc
    10321 tggcggctct actccttctt cataatcaaa ttcatcattt cgtccgattt caataaccgc
    10381 tgtgttgtta tggaaatcat ccgcttctag ttctgtggaa accttcaaat tatacgtgat
    10441 cgttaaatct cctccacgtg catctgctaa tctacctaaa gtatctactt tggctactcc
    10501 attttcttct gtcaaaatcg tcacgacaaa cccgttgctt actacttcta ctatataatc
    10561 ttgtcctttt gttaaaatcg tctcgccggc tcgcacttct atttcctgat cataataagt
    10621 tagaccaact gtaggcgtat ctgtgatttt gtaatttttg ataacagcgc ttccgttttc
    10681 aaggacagaa tcgatgaaat aaggaatagg tgctgtgatc gtgtaagaag ccgtttttcc
    10741 tctttctaga tcaatataag aaatcgtttc ttgttctcca gcttcattaa taatcgttac
    10801 ttgtctaagg tcgctttcct ctacattcat ctgtttatct gcttctctga tttcgttttt
    10861 cggatacaaa tgcacatctg tattgtatgt gtctttccat gtagaaccat cccacacgcc
    10921 atccaccata tcaggcatca tcactggcat cataacaacc attggagacg cgattctgga
    10981 aatatgagct ggcgaataag tttctaagaa aagatagact ttatctctag aagaaggtgc
    11041 ttcttccgtc acttggacgg tagagaatac cgcttctcca gcttcattcg ttgtttgttt
    11101 gtcaatactt gtcagtcgat attttaaaaa ccaactttct gtgtgccgtt tttgaatcca
    11161 ttcaatcgct tcggcagaat taagaccgtc ttctcgttcc ggtgttaaag gattatctcg
    11221 aatcaaatcg taatatactt ccgttacgtc aaacaaatca aaatcgactc ctggaatatt
    11281 ttcttcacca aaatccgtag aaaccaatcc gctattcggt cgttcttccg gaacagatga
    11341 gaaacctttt ttgtgtaatg taatcgtcac ttcacttggt ttttgtgcga cttcttcggc
    11401 tttgactagt tttccgttcc cgaaaatccc tattcccgca gtaacgagta acagtacact
    11461 cattcccgtg acaatcattt ttttattcat ttttttctcc ccgttctttt tttattaata
    11521 aaacagcacc taacaataaa agaattccta gtaaagaaat caacgttctt gcttcattcg
    11581 tttgcgggaa tacggaccat tttcctgatt gacctggatt gcttggacca ctcggctctt
    11641 tcggtttgtc cgttgtttct tttacggtct cgttataaac acgtggtgtt gcggattcat
    11701 aggcagagac tggaactttc ccggattcta attcagccat ctcctgtccg ttaactacta
    11761 catattgtgg ttggcctttt gcatccaccc aaaattcagg gatgatgacc tcgattctct
    11821 tacttgcttc atccatctcg taaccttcca caccttttaa ttcttcaaaa taataggtgc
    11881 cagaaggcaa gaaacgttgt cctgtcgtaa caaggccctc tttatccgaa atgaatttgc
    11941 ttacattttt gtcattcaaa ggatcattgt ctgctggttt gatccattga tttttcaagt
    12001 cattagcagg agccatatct aaataatatt tccccccttc tatcatctga tatagagcaa
    12061 aaacagcacc ttctaatggt tttccttttt cagttgttcc tctttgacgt ccatacttga
    12121 agaagtatgg atctcttaga taaccgacat ttttaggata gatatgaatc tcttgcagtt
    12181 cctcggttga ttctgtcggg tgataaattg gcaaaatagc agcaattggg atagctgtct
    12241 gatcgatatc aacatccaat ccaacttctg gatcaatcga ttcttcgaaa atcaaataaa
    12301 ctgcatcccg atcttggttt ttcttaggtg ctttgatacg tgcaatcccg tctcctaatc
    12361 cgccaaccgt atctttccca gttgttaccg aagtccctat ttggacaagc ctttcttctg
    12421 tgattagctg acggatattg tttctgttcg tttcagcgat ttgctggacg aattctttag
    12481 gagtcatgtt ttcagcagct tttttttcat gataataatc ggtcatgtcg taaatggcaa
    12541 atgtcgctcc atttagtggc accgtctgtg aaatgatatc tgctgtttcc cctgcttcct
    12601 cagagatagc caagccatca ttttcatggt attcaaattg atcgttataa tcgatatctc
    12661 gaatcatccg tttatgcaac acgaattcca cgacatcttc ccctgattct tctgccgaca
    12721 tcgcatgtgg aagaaggact gctgcaagcg gtagtaaaac aagtaattga agcatcaaac
    12781 acaacagctt tttcattacc ttgctccctc ctttttagat tgtctgacaa acaaaccata
    12841 ggctgctcct gcaagcgcca tgaagaatgc agcaagcagg atatagaata atcgtccgga
    12901 tcctcctgta gcaggcaacg gattttcagg atcgttcccg acactaagct gtaatctgat
    12961 tcgatttgtc tccaaaagtt catattctaa ctcttgctgt agatcaggcg tctctgcttc
    13021 ttcgtttgga taatacgaaa tctcaacttt tagtgtaccc gcttcttttc ctggaactac
    13081 ccgatcattt tctatgtcag gttcttctcg ataactaatt tctaaaataa agtaacctgg
    13141 aagttttcta tagctgtcag gtgacttcac ttccttgatc cgatagaccc cttccaacaa
    13201 gtctgaagca ttgaataggg aagaatcgtt tggatctggt gccaagcctc cctcgatgat
    13261 ttcatactct cctgcactat tttgtttctc aattgaaaat tctgcgcctt caagacgtcg
    13321 gctattcata ctgtcaattt tcaaaagttc caaatcgatt ggcgctagtt catcttctaa
    13381 ggttacttgt agtacaccat cgatgatttc atagccttcc ggcaagggag cagtttcgtc
    13441 ttcgatgacc gtatcttcaa tgataaactt accttcttca ttcagtgcaa actggaacac
    13501 ttgatcagat agccgatacc catttggtgc gacagattcc gagacctcat attcaccaat
    13561 agacagatgc agaggttggt cgtaatttaa atcgacatac ttttctactc ctacggcacg
    13621 agaatagaag tatccgatcc caggcaaacg tttcgattct tctggttcta acggattacc
    13681 agtgtatgct cgatacagcg ggaaggaaga atcccccact ttcctgatat cgaaacctgc
    13741 accatctaat gtatggcctt catggtctaa ttttctaact cggatctccc gataatcaaa
    13801 cgtattgtaa gcagtgaatg ctacccataa acgatcctct tcatctgttg tatacgtgag
    13861 atattgccaa ttttcttcac ctgtttgctt gagttggata gtccctatag cattatcgta
    13921 aagtcgcaag atccatgtgt cattttcatc aggttctttg aaaccttctg ggttctggct
    13981 ctcaatgatt ttgtattctg catagttggt agtttcttca ctatccttat aaacaaatga
    14041 atctggctct ccgattggat gaataacttt ctcaccatcc acgattacat atttcccgtc
    14101 ttcatctact gaatagaacg tgagtctccc tttctcttcc tctacttcgt tcacaccaga
    14161 aagccctttc gctactggat atccgatttc cagtccgtta ccatctgttt cataaagttc
    14221 aaactctgcg ccattgattg gcatattact gccttcaatt agtttattga tattcatgtg
    14281 gattggcttg aattcatttc gcatttccag tttgacatac aactcatcat actctgtgcc
    14341 ttctatgtta ggctcttgta caacagaaag cttgtgatat tctccttcat aataggaatt
    14401 cggatcttta ccttccggtc cgccgactct cgaaccagat gtatattcat ctacaagctc
    14461 gtagtgacct aacatctcat cttcgttggc tgcatatttt ttgatttgga attgatacgt
    14521 ctcatctact aactgataac gtggtggtcc agcgatctct ctgatccaaa atatgtcgct
    14581 gactgctcca gtttcatctc ctaaaatcgg catcttcgtt tcaaagatcg ctattccgtc
    14641 cgtggtagtt ttacttgttt cgattggttc ggcttcccca ctctctggat tttggtcata
    14701 taatgcaaat tcgactccag ccaacggctc gccattttga cccttcttgc tgacttggaa
    14761 attacggcct ttatgttcgt aacgaatgga agaagatggg aaatacatcc gtccagtagt
    14821 gatatcattc tcataatcct ccaggttctg aatataggcc atattgttca ctgtgcaaaa
    14881 atttccgtct tgagctcccg catcaagttt tacttggtac ttgatcctga ccatttcccc
    14941 ttcacctaaa gaaatcggag aaaatttata agcttggttt tcccgtccga catacgttgt
    15001 cggagtcgcc actacttctg gtggggcttg tccatgacgt tcttcatccc atatttctgg
    15061 ctcttggtac ttcatgccgt tttctcctgg atctagatgg taaaactgaa tatctgccga
    15121 tctgccagct ccaaatggtg agacattaaa accagtttca tcataaagtg tggcttgctg
    15181 gatcgttctc ttgaaggtag aagagatatc ctccaaggct gtaacaatgt cattcttgtt
    15241 gttcgcattg taatatcgcg gtgttacgcc atcttcttct aaatcagcaa tatttttcaa
    15301 tacatttcgt ccaatagcat ctagtcgttg tctcccgcta acgtttccat cgattcctaa
    15361 accaatggag aagatacttg gcgggttctc ttgatacttg tttttcagcc aatgatgata
    15421 agcaactgtg ttgatcgcag atgacttttc ttgcgaagta ggactgccat tcgtattgtt
    15481 attagaagaa ccatcaccaa cccagtgagt cgctttatta tctccttctt ttacctctcc
    15541 ataacgatag tgtacattta cttcatcttc tggacggtta aagtcagatg cataggtaac
    15601 tggatgagta aaccctgcat tagaagtatt cccagaaaac tcctcaaaat ttctgaacaa
    15661 aacgtcatta tcttcagcaa tcttatcttc catcacagtc cagttgctcc aagctccatt
    15721 agtgctcgta cgataacttg actgaatcgg tgcataactg aaggtcggtg tcccatctcc
    15781 cacaacgatt aggatttttt ctggatttct attttctccg ccattgtctt tatacaatgt
    15841 ttcatagcca tttttaagcc ctagtgtaag cggagttcct ccgattggtg tacgatttaa
    15901 tgctgtattc tcacttcccg tcaaacgatt gatatcattt gtcaatggaa agatgtccga
    15961 actgatttgc tcatggttat tgatagctgt actattatgg tgaaaattca ccataccgat
    16021 tcgaatatcc agactcgggt cagagagcag atctttactc atttcgttta ctgcctcaat
    16081 caacgcagca tttttagttt gactattggt cccagcggtt aattcactca tactcgctga
    16141 cttatctaga acaaaaacga tatcgattgg actgatttcc tttttctcgc ccccgatgac
    16201 atctagatag acattgtaaa gggttgttgc atcttcagga tgtagatcag atgctgtgct
    16261 gcttggcaga acaggttcta cccatttttt gatgtacgcc ccgttataat ccaagtaact
    16321 gttttcaaaa ttccgttcat ttccccaaag ctgaacggtt tgagctggaa cgtttctatc
    16381 ttcttcgcct gcagttgatc cattattagt agcatagtcg taatttcgcc agttcgctcc
    16441 cccgtttgta taatcactat catcgatcaa gttctctccg tcatatggcg agcctttgtc
    16501 tccttgatag cgttcattgc tttgtgtcca atttcctaac acttttgtat agtcattcgt
    16561 tgtaaacttc gggtagcgat gctgttcgtt ttcaacttct ccgctaggat agatatattc
    16621 ataggggtct tgcgtagctg ttggcccaag acgctccaaa ataccgttga ataactggcg
    16681 ttgaatgcgc tggagtgctt gaccgctggg tacgattggt tcgtccagct tttccatctc
    16741 attttcttcg ctacgttgaa tttcagcgga atcttctgta tcagtttcat tttcttgtaa
    16801 ttcttggtta tctattcttt catcaaccac cacttgaaga ggtagttacc cttcgttcac
    16861 atcggggatc ttttctgttt caaaaatcaa tgtgccggtt tctgcttctt ttgaataaac
    16921 ttttccgaca taccattctt gaccatcgat cgtctcgact tttaggtctt ctccatcttc
    16981 atcataggag gatagacctg ttccacgaac gttttttacg gtaccaatcc cggcggctgc
    17041 cgcatcgatt gctaaacgta ttctaccttc attggcactt tccttttttt caaaagccag
    17101 atgccattca agggtttctt ctttttcaat atacttccct tcaatttttg catagtcctc
    17161 atctaagaat acctcatcct cctttgtttc tgttgactca gcagctacat ccagggtcgt
    17221 catatgatag actgactgga aatatggtcc aaatagcaaa ctgacagtta ctaactgcaa
    17281 aagcttcaat tttaaccctt tcacatcctt atactccttt caaaaatatt tgtcttcttt
    17341 cacaacaatc aacaaaacaa tgcccatcta attataattt aatttaattt taattttaaa
    17401 tcaaatgata tcctttttag ttaaaaaaca ccaattatac aacatttatt attaaaatta
    17461 aattataaaa ttttataacg ttctcattta acaaaactta agttattaag acaaatgaat
    17521 aaacaactac gctttcctcg aaacaaaaaa ataaagcaca aatgcgcatt tacgcactca
    17581 tgctttattc gtacaattaa ttcagataac ggcagcgcat aggatgccaa taacacttcc
    17641 taaaatagct cctcctaaaa tgtcagtagg ataatgtaaa agtaaaaaaa tcctcgaata
    17701 agcgatcaaa caagcaaata aaaaggctaa tattcctgca actggactta attcgaacaa
    17761 cgcagctact gataaaaagg ctagcgctgt atgtcctgaa ggaaaagaag aagtcttcgg
    17821 tttacttgtg taaaggacaa gccgacgatt tttttcataa ggtcttttcc gtcttgtcgt
    17881 ttttttgata aaaagcaaac caacaaaaaa atagatcaaa ctgccgataa gaatggctag
    17941 accatattct ctagtatcag gtatcaagac taaaacagca gcaaggagca accaaataaa
    18001 accaccatta gagatattac tgataaattt caaaaaattg atttttcgac tatccgtttt
    18061 ccgttgcttg gcgaacttgt tgataatact atcatcgatt ttagcaatca aacgcaacat
    18121 gttattcctc ctttttaaga accttttctt gcgtacgatg gataaaaagc aggacttgaa
    18181 aataaaacaa gaaactgaaa cctaataaag caaaatagat tggtcctgca aaccagaatg
    18241 cgacaagaag gatcaacgtg cctgccaaat agaaaagcgc gcttttgaaa tcattaaaaa
    18301 acaagaaaaa tgcagtaatc caagaagctt ctttttgttc cagttgaagc aagattttgt
    18361 aactaaagag aagcgcgctt aatagaagca aaaacaacgt agtatacatc aatgtatagc
    18421 caattccccc accccaatag cggaaaatct gataatcgat caatagaaga agaaaataaa
    18481 cacttgctaa actatcttgc tttccataaa gagagaagtt ctcacgaaac tttttccaga
    18541 atagtgaacc gagctttttc tcttcatttt ttttgtaatg ataaatcata tacagaactg
    18601 ttcgacttgc agttcctaat gtgaaaacag gcaagacgca caggaaccat agtagctgta
    18661 aatggataag acgtggaatc cattcaatga tttctaagaa ataatcattg gcttttttac
    18721 caatcgatcg attcatcatc cttttgaacc tcctgtcaaa tccatccctt caatgaaata
    18781 acgttgtgca aaaaagaaca gcaaaatcgt cgggatcaag acaagtgttg ctcctgccat
    18841 cagataattc cattgtgtcg ttgcttggtt ctgaaaaact tgcaagccaa tctgtaacgt
    18901 gtatttttct tgatcttgga tatataacag aggacctaag aaatcattcc aagctgcgtt
    18961 gaaggaattg atcgcaatgg taatcaaggc aggtttcgtc aatggaagca tcaaatgact
    19021 ccaaatatac aaatgattcg ctccgtcgat ttcagcagct tcgatcaatt cattattgat
    19081 cgataagtaa aattgacgca tcagaaatat attgaacgca ttgccgaaga acgatggaac
    19141 gatcaggggc aaataagtac ctacccagcc gatcttggag aataatacat attgagggat
    19201 catcgttaca aaccccggga tcatcatcgt agaaagaacc aaagcaaaca tcagcttttt
    19261 cccaggaaaa tcaagcttcg caaatccata agcaataaaa gaatttgaaa ggacattacc
    19321 gatcactact agcacagtga tgaataatgt atttcttgca tagcgccaaa aaggaaaggc
    19381 ggcaatcgta tccagatagt tttcccatac gattttttct gggaaaaagg tcggaggata
    19441 acgaacgatt tcttccattg gtttcaatga agtaaacacc atccaaagca atggaatgat
    19501 caaaatgatt cctattactg tcatgaggac ataacttagt attttttttg tttgccgatt
    19561 cacactggtc tttttgaaat aatccatcat ttgccgtcac ctccagaata atagacccac
    19621 ttaggtgcaa gcttcaaatt gacaaacgtc agaaccaaca cgatcagaaa caggatcatt
    19681 gacatagcca tcgcataacc catatcaaaa gctacgaacg ccttgttcca catatgcaga
    19741 ttataaaaaa gcaaagaatt tgcaggtccg cctgttccat ttttcgtcat gacataagct
    19801 tcttgaaaaa tttggaaaga accgatcgta gaggtgatca catcgtaaaa aataatagga
    19861 gtgattaatg gcaaagtaat acttttgaac tttccccaaa cgcctgctcc atcgatttct
    19921 gctgattcat ataaagatct aggaacattt tgtaatgtag ctaagtaaag tagcattgct
    19981 cctccagaac tccatatttt catcaaaatc aacgcaggtt tcgtccattg tggatcaaac
    20041 agccaagctg gtccagggat atggaaccag cccagaaatg tgttgatcaa tcctgcagat
    20101 ggagaaagca gctgcatcca taagatgtag actgctactc ctgataagac tgccggtaaa
    20161 tagaaagctg tacggaatac actgattcct ttgatttttt gattgagtac tgtggctaaa
    20221 aacacactga aaatcgtcgt cagcggtaca ttgaagatga cgaaataaac ggtgttgtat
    20281 aacgatttcc aaaataaatc atcgtgaacg aacattcttt tgaaatttgc tagaccaatg
    20341 aaattcatct tggatgtcaa attataatcg gtgaaactac caataactga aaaaagcata
    20401 ggccctagca taaataccaa gaacccgata ataaaaggcg agataaataa ataggcccat
    20461 cttgcttctt ttttctttcg gatagataaa cgcttcaaat tttcttgcct cctctgtacc
    20521 tagacagtca aaaatggatt attgattttg cttgactaag ttttctactg ctttttgtgc
    20581 atctgccaat ccttcttttg gtgattttgt tcctaaaaga atctggtcaa tctgttcatt
    20641 cactaatgtc gtgtaatccg gagcatctaa aggaactggt gtgatcaccg tatttttaaa
    20701 gttttcgtcc gccatttgat agatcatttt tcctttatcc gttaggttgt cattattcac
    20761 taaattctca ttggctgtct tgctcgccat gatatcaaaa cttttttcgc cgaatttttc
    20821 ttgtacttct ggagaagaaa gatatttgat gaattcataa gaggcctcag gatcttttgc
    20881 tccgtatggt acttccaaga cgaatccgcc accccacgac caatggcctg aaccttcttc
    20941 tttttctggg agctgcatca cgccaaaatc aaaatcatcc ggtgcatttt ccatcaaact
    21001 agagtaataa ttgatatttt gggcacgcat ggctactaat ccggaaataa atggatcaga
    21061 tacaccagac ccaaattcgg cttctaattt attgatcgta tttttcccat aataatcttg
    21121 ccagtcttgg atccactcca aggcttccac tttattttta gtatcaatct tgactttttc
    21181 atctttgtca aaccaactag tcccgttatc tgcgtttagc gcccatacat cagcccctaa
    21241 attccataat ggataaaaac cgatgcgtgt aaattctttt ccttcttttt tatctagttt
    21301 acgtgctgtt tcttctaatt catcccaagt tgtcggtaga tcttgttcac tgattccagc
    21361 ttctttaaat aactttttat tataaaaaat gacttgtgta tctgtattga atgggatcgc
    21421 ataaggatca ccttcatatt ctactgtatc ccaaagctgc ggataaaaat ccttagaagt
    21481 gtctttttct acatatttgg agagattagt tgcttgctgt gcttctgctc tttgggcaac
    21541 ggaatttatg tcctgtacaa taacatcggg tgcatttcca gctgttattg cagataaaga
    21601 tttagtccag atgtcacccc atggctgata cttataattt acatggatct cgttttgtga
    21661 ttgattgaaa tcctcaatga tttcttcgat agcttctttt cgtgcaccgg atccccaaaa
    21721 ggaccagaaa tcaatatttt ttcttccatc acttgttttt tcagaagaat ttgctgcact
    21781 tgaactggca tcgcttgcat tacttgatcc gcatcctcct aataaaaaca atgctgaact
    21841 tgccagcaac cctgtaaaca ataatttttt catattttac ctccaaattt taatgtaacc
    21901 gatgccggtt ttaatataac gatatcattt tgttctgaaa ataacaattt ataaggtatg
    21961 attctaaaaa aattattaat gataccaata ctttatataa ttcgcgagcg ctttctttga
    22021 attttttaat ctttcgtagc agaatgagaa tgtattaaaa atagattagg aggaattttg
    22081 atgtttagac accagaaaga attgcagttt gaagcaaaac cagatcgtcc gaatccatta
    22141 atcgcgaaat atctgcagga gctgatcggc ggccaatatg gcgagatgag tgtagctatg
    22201 cagtacctgt tccaaggctg gagctgccga ggagaagaaa aatataaaga tatgctgatg
    22261 gacattgcaa cagaagaaat cggccacgtg gaaatgttgg cgacaatggt tgctcgttta
    22321 ttagagggtg cgcctgttga agatcaagaa gcagcgatgc aaaaagatcc aacaatcggt
    22381 gccattatct ctggaatgaa tccacagcac gcgatcgtag ctggcttagg acctcgtcct
    22441 gctgacagcg tgggcaatcc atggagcggt gcctatatca ttgcgagtgg caacttgtta
    22501 gcagacttta gagctaattt aaatgccgaa tcacaaggaa gacttcaagt agcacgtatt
    22561 tacgaaatga tcgatgatcg tggcgtaaaa gatatgctgt ctgttctcct tgcaagagat
    22621 agctatcatc aaaatctatg ggaatgtgca gtgaaagaat tagaagaaaa agaaggttca
    22681 atcttagtcc caagtacttt cccgcgtgac aaagaacgaa aagacattgc ttatgacttc
    22741 tataacttct cagagggaga ccaaagtagt gaaggttctt gggctagtgg caaagcactg
    22801 gatggcgaag gcgaatatca atggttgaaa gaacctaaga tcgaaggcgc tgcaccaaaa
    22861 ttagaaccag taacaccaaa aatgtatggg acacctccat tcaagaaata aatatctacc
    22921 cgtagaaaca cccgtttttt gttccacacg ctttgtgagc aaaactgact aaagtcatta
    22981 atcataacca cccttcaaaa gggtggtttt ctctagagct ataagctcaa atcaccggcc
    23041 agcgcctaaa gacgctggct ttcatatttt atgttcaagc catattgcct ttgccagttt
    23101 tgcccacctc ttaaaaggtt tatgtactac tccttagccc ttaaaaggat cgtcttactc
    23161 tcgtacactc agcttatcta acgcactatc atgtttttct tgttcttgaa tgtacttttt
    23221 gatggtcgct tcattttgtc ctacttttgt cacatagtat ccttccaccc aaaagtgtcg
    23281 atacccaaat ttatatttta gatttgcaca atctcttgat gatctcctga atattggctc
    23341 gatattgatt ataaattatt tttcttctat actttggtgt gaatacaatg tggtatttac
    23401 acatctactt tgtacgggct aaactatttc cctttattgc tccacccgca tagcaagtgg
    23461 ttttgtattt caatcgctcg gcgcgctcaa ttaggtctaa agacaataaa tctaaaaacc
    23521 cgctgcttca ctgacagaaa caacgggttt tcagaaaaaa atgaaatgaa aaagatctct
    23581 tacggcaatc tattttattt ttttgatttt ttataaatgg aaaatgcttc aagaagtcca
    23641 agaaataaag ccgtataaaa acaagaacct atgaaatctt tatttattag atattcaaca
    23701 gaaatcccga taacactagc caacaatata tttatcagaa gtgtaaaata aggatgcttt
    23761 ttatagaata gaactagtgt tgttttcata gactctcctt tcttcatatt tccaattaat
    23821 cacaaattca cggcattact agaaaaatta aaggggcgca gaagtggaaa aatactcttc
    23881 gactttccct acaaaccagg ccatcttgtg tttaataact ataccaaatc taaggtacct
    23941 ctaaatctaa attccatttg tggcggtgac ctccaatttt taactcttct atctcccatc
    24001 cagtatacat aaccttgata cttcgaccac ttatatatcg aacacacaaa tacctatcca
    24061 ttgcaggagt actacctaac ttagttgcta cttgcatcta aataacagct gaaaagatag
    24121 gacttactct tagtaaagta tctgaactat taatttttaa actatcattt ttatcctcaa
    24181 ctacatgcac atctgttgca acttcacttt ctgtctcatc cagataaatg attaaggaat
    24241 cgggaattgc aatatcttca gctgacgctg gaatagttga gctaaattag tcatcatatc
    24301 tttattataa aataaccaac agcaaaaaat acaatgaaaa acaaacttgg aactaatgta
    24361 tcattaataa atgttgaaaa attcagcaac tcattactgt atgattttag cagcccaacc
    24421 aacatatacc ctataaaagc taataagatg gttaggaatc ctttagaatt tttactcatt
    24481 catagacact tccttaaata acttttatta tagaaggtat tgtaaataat aatattgaca
    24541 aaaagtgact caaaataaaa aaaaccttac attatactgg aatgaac
//
LOCUS       DAMEET010000039        24269 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.039, whole genome shotgun sequence.
ACCESSION   DAMEET010000039 DAMEET010000000
VERSION     DAMEET010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 24269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.039"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..572
                     /locus_tag="PB780_001396"
     CDS             <1..572
                     /locus_tag="PB780_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDE0733457.1"
                     /translation="ASRIAIMSEGRVIQFDTPENILRHPANDFVEELIGEDRLLQAKP
                     NATRVGEVMLNTAITITPEKSLQEAIKLMREKRVDTLLVTDNSNVLKGFIDVETLNKK
                     RGKVSSVGDILNKHVFYVKESAYLRDTLQRILKRGLKYVPVVDEQNKVVGILTRASLV
                     DIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            576..1217
                     /locus_tag="PB780_001397"
     CDS             576..1217
                     /locus_tag="PB780_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDE0733458.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            1214..2131
                     /locus_tag="PB780_001398"
     CDS             1214..2131
                     /locus_tag="PB780_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HDE0733459.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            2128..2799
                     /locus_tag="PB780_001399"
     CDS             2128..2799
                     /locus_tag="PB780_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDE0733460.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3340..3828)
                     /locus_tag="PB780_001400"
     CDS             complement(3340..3828)
                     /locus_tag="PB780_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HDE0733461.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4029..4295)
                     /locus_tag="PB780_001401"
     CDS             complement(4029..4295)
                     /locus_tag="PB780_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDE0733462.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4295..4558)
                     /locus_tag="PB780_001402"
     CDS             complement(4295..4558)
                     /locus_tag="PB780_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDE0733463.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            5034..5219
                     /locus_tag="PB780_001403"
     CDS             5034..5219
                     /locus_tag="PB780_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDE0733464.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(5361..7184)
                     /locus_tag="PB780_001404"
     CDS             complement(5361..7184)
                     /locus_tag="PB780_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDE0733465.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7782..10487)
                     /locus_tag="PB780_001405"
     CDS             complement(7782..10487)
                     /locus_tag="PB780_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDE0733466.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(10793..11740)
                     /locus_tag="PB780_001406"
     CDS             complement(10793..11740)
                     /locus_tag="PB780_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HDE0733467.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            11930..12199
                     /gene="rpsN"
                     /locus_tag="PB780_001407"
     CDS             11930..12199
                     /gene="rpsN"
                     /locus_tag="PB780_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDE0733468.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(12375..12737)
                     /locus_tag="PB780_001408"
     CDS             complement(12375..12737)
                     /locus_tag="PB780_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDE0733469.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(12748..13869)
                     /locus_tag="PB780_001409"
     CDS             complement(12748..13869)
                     /locus_tag="PB780_001409"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDE0733470.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(13885..14235)
                     /locus_tag="PB780_001410"
     CDS             complement(13885..14235)
                     /locus_tag="PB780_001410"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDE0733471.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(14365..15876)
                     /locus_tag="PB780_001411"
     CDS             complement(14365..15876)
                     /locus_tag="PB780_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDE0733472.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(16198..17550)
                     /locus_tag="PB780_001412"
     CDS             complement(16198..17550)
                     /locus_tag="PB780_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDE0733473.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(17703..18779)
                     /locus_tag="PB780_001413"
     CDS             complement(17703..18779)
                     /locus_tag="PB780_001413"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDE0733474.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            18975..20720
                     /locus_tag="PB780_001414"
     CDS             18975..20720
                     /locus_tag="PB780_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HDE0733475.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(21000..21437)
                     /locus_tag="PB780_001415"
     CDS             complement(21000..21437)
                     /locus_tag="PB780_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDE0733476.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(21421..22794)
                     /locus_tag="PB780_001416"
     CDS             complement(21421..22794)
                     /locus_tag="PB780_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733477.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(23116..23604)
                     /locus_tag="PB780_001417"
     CDS             complement(23116..23604)
                     /locus_tag="PB780_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDE0733478.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(23626..24228)
                     /locus_tag="PB780_001418"
     CDS             complement(23626..24228)
                     /locus_tag="PB780_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDE0733479.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         6959 a         5162 c         4488 g         7660 t
ORIGIN      
        1 tagcaagccg tatcgccatc atgagtgaag gccgtgtgat ccaatttgat acaccagaaa
       61 atattttacg tcatcctgca aacgatttcg tagaagagtt gattggagaa gatcgcctac
      121 tacaagccaa gccaaatgcg acaagagtcg gcgaagttat gctaaataca gcaatcacga
      181 ttacaccgga aaaatcatta caagaagcaa tcaaactgat gagggaaaaa cgtgtagata
      241 cgttgttagt taccgataat tcgaatgtat taaaaggatt tatcgatgta gaaacgttga
      301 ataaaaaacg aggaaaagta tccagtgtcg gcgacatttt gaataaacat gttttttacg
      361 taaaagaatc cgcctatctt cgtgatacct tgcaacgcat tttaaaacgc gggctgaaat
      421 acgtgccggt cgttgatgaa cagaacaaag tcgtaggtat cctgactcgt gcatcattag
      481 tggatatcgt ctacgatgtg atttggggag aagatcccgc aactactgat gttgcagagt
      541 cagctactcc tgaagaatct aaggaggtct gacctatgca gcagtttttt catcagcacg
      601 gttcagaaat tctctcaaaa ggttttgaac atctttatat ttcaggactt gctttgatca
      661 ttggtatcat cattgctgta ccgcttggtg tcttactgac acgttttcca aaagctgcca
      721 cgattgtgat tggcttaacc agtgccttgc agacaatccc ttctcttgct ttattggcat
      781 tgatgatccc gctatttggc gtaggtaaat tacctgcgat tatcgcactc tttatttatt
      841 cattattgcc gattttacgt aatacgtaca ttgggatgaa aaatgtcgat tggaattatc
      901 gggatgtggc aaaaggcatg gggatgactg agttccaatc cattttttcc gttgaattgc
      961 ccattgctat gccaacgatc atggctggta ttcgtttagc tgccgtttac gtgattgcat
     1021 gggctacact tgcctcttat atcggcgctg gtggtttagg cgatttgatt tttagtggac
     1081 tgaataatta ccaaccagat ctcattttgg caggtacgat ccctgtaaca attttagcac
     1141 tattggctga ttggcttcta ggtctcttgg aacaccggct gacaccgatc gcacttagag
     1201 aggagaatga agaatgaaac gtatactaaa aatgaccatg gttcttttct ctcttcttat
     1261 acttgcgagt tgttccttcc ctggtcttgc cagcaataca gatgaagaca ccatttcgat
     1321 cacaggtgga atcacttcag aagcacagat cttagcaagt ctagttgccg gaatggttga
     1381 acattataca gataaaaata ccgcaatcat caacaatctg gctactacaa ctatcaacca
     1441 tcaagcgatg atgaacggag atgcttcaat ttcagcggct cgctacacag gaacagattt
     1501 aacgactaca ttgaaccttc ctcctgaaaa agatccgaaa aaagcgtttg ctaccgtgaa
     1561 agatgaattt gagaaaagat acggtcaaac gtggttcccg tcctatggtt ttgaaaatac
     1621 gtatgtcttt cttgttcgaa aagacaccgc acaaaaatac catctatcta aagtcagcga
     1681 tctgaaaaat gtagcggatg aattagtagc tggtgtagat acttcttgga tcaatcgtaa
     1741 aggagacggt tatgacggct tccaagaaac ttatggcttc tccttcaatt cgatattacc
     1801 tatgcagatt ggacttgttt atgacgcagt cgaagcagga aaaatggata ttgttcttgg
     1861 ctactctacg gatggacgga tcgccagtta tgatctagtc atgttggaag atgatcgtca
     1921 attcttccca ccatatgatg ctgctccagt tgtagacaac aagatattag ctgatacgcc
     1981 tcatttaaaa gaagccttaa ataagttagg tcataccatc agtacggaaa aaatgcagga
     2041 attgaactac gaagcagata ataatctagt ggaaccatca gtagtagcac aacgtttctt
     2101 acaagaaaac cactattttg aagggaagtg aagcaattgg ataaaatgaa tgtctttcaa
     2161 caatttttct attatttcca ggaaaatggc tcctatatct ttgcacaatt tatccgtcac
     2221 ttcctgattt ctatctatgg tgtcctcttt gcagcagttg tcgggattcc ggttgggatc
     2281 atgatttcta ggagacgtaa attagcaaac tgggtgattc gtttggcgaa tatcatccag
     2341 acgatccctt ctctagccat gatctccatt ttaatcattg gattaggcct gggagtcaat
     2401 gtcgtgattg ttactgtatt tttgtattcc ctcttaccga tcatcaagaa tacgtatacc
     2461 gggatgatcc aagtagacaa aaacatcctt gatgtcggca aaggcatggg gatgactgct
     2521 agacaacgac tattcatggt agagttacca ctctctgttt ctgttatcat ggcaggcata
     2581 agaaacgcgt tagttgttgc catcgggata accgcgatcg gtgcattcgt cggtgctggt
     2641 ggtcttggag atatcattat ccgaggaacc aatgcaacag agggcacatc gattatttta
     2701 gcaggtgcat tgccaactgc attaatggcg atcatcacgg actggctttt aggtattttg
     2761 gaaagacggc tagatccagc cagccgtaca tcacgctgat gttattgtaa aaaacaaaag
     2821 cttctgcgac catttgttgg tcgcaaaggc ttttttagtt ctgtcatttt taaggcaata
     2881 atccactctg attcttccct ccttatgggc aaagagttgc ttttcttagc tatttatcca
     2941 ttccgtatac tccaacttca tactgccaac gggttcatat ctgcatccac cgactaagtt
     3001 tttttcttat ccgctaaggc attttgccaa tccattttcc catcactcgt catggaattc
     3061 ataccacatg atctctaaat actcgtctgc ttgttctttg aattgggcat caattcttac
     3121 atcctttggc gtggctttca tttaagcctt ttccatcagt cctcgattgt tcgatgcctt
     3181 cagctgcatc atcaaaactg gtgactgccg tatcggtaac ttcgatcatc ttttatacaa
     3241 tcgggaactt gccatcctca tccttggcta aaaacatatc tagccattcc agcttctcgc
     3301 catgggaggt ttgcggttcc taaaaccggt acacgattct tatggactgc atccgtcgca
     3361 actagacttg gcgatacgat gatccttcac cagaagagca cccccgtaaa ccaattgatc
     3421 gatatattgc caataagaaa agacattggc gtaaaagata tcgatccctc gtgatgcatt
     3481 gccgttgata ctgctattca tggccgaaag cgccacgact ttaacttctt ccgtttgagt
     3541 gacattcatg ttcggcaatg tcgctttgga tacccgctct gctaaaggaa cgacactggt
     3601 attgtatttc gtatctgtat cttttccaaa agaccatgcc aacgaatcct ctgggaacca
     3661 ataagacgac tccggttgat tgtccattgc ttttttcaac atacttttgg ctgtttctcg
     3721 ataaacgaca tttccaattg tcttcgcctc actttggcga taatcgatgg tcgttgccac
     3781 tgccaacact aacaacgatg ctccaatttt actgattttc cttgtcacac ttcttcctcc
     3841 ttgttttaca taaacccgtc aacattccat tcctaaactc aaacaatgac ccttcaaagg
     3901 tggagatata ggaacaaatt aaagaaatca tgcaataaat ccaagaaggt tggttttaga
     3961 acaaaaaaag agtctccttt ttggggagac tctttgttaa ctatctaaaa aggttggctc
     4021 ttctcacttc actcataatg tgtccaggca gaataaattt ctacaacttt attttcctca
     4081 tcaactttgt aaaccactcg atgctgtcta ttcagcctgc gtgagtatct gccttcatgt
     4141 gtaggtctca gcttttcaaa agactgcgtg ggcatatatg gatcttcctt caatgtctga
     4201 atcacttctt caaattgttt ctttaaattc gtttgcttga tttttcttaa atccactttc
     4261 gcagagtttt tgattgcgac tgtatagtta ctcatcaaag agcttcccaa tcaatatcat
     4321 cgatattcgt aaagctactg tcgtcttttt cgcggtctcg tactttatct aaagtacctg
     4381 tttgctcaag aaaaagggtt tcttgaatcg ctctccagtc ttccagccca atcaatacag
     4441 cattgtttcc actacgatca ctaataatct cgatttcagt atgattttca tttacctctt
     4501 tcaataactg ataaaaattt ttacgagcag tgctaggatt catcgttgta ttagccattg
     4561 tctctcctcc tttttctgat ataattatag cgtacttgat taagtacgtc aaaaaaatac
     4621 aataaagcac ttaaattcag caaacttttc ctctttttta catagagaaa aagacccctt
     4681 tttagggagg atctttgtcc acgctcactt cttactcctt tacgactagc ggtagccaaa
     4741 cgtaaagtaa ttggtaacaa cgtatcatct ttagcaaagg tcgaatccaa agcctgtgag
     4801 tctatgcaac aacaccaata cacgaaggtt cctcgttgca gtgtgacttg attgatatct
     4861 tgaataacct gaggatacgc acgaaagatt tgtaacggtt attcaaaact gattttggcc
     4921 gtcaagtctt ctactgcctc aaaaatgatt gcctctttcg tctcctgtca ttcatgttca
     4981 ctatcgattt gatacttgct tagaccgttg ggtttgtgaa tcaccaatta aaaatggcac
     5041 tgagttatgg taacaaaaaa tggtcacttg acgaggctga gattcctact tatgaagaca
     5101 catggcactt aagagtcgag gtaaaggaag aagctgaaaa tataggaggc tgtacattat
     5161 ttggcaaagg attcggaaat tatgatcaag acatcaaaat caaaatgttt tacagctaaa
     5221 taggaagaat aataacattt catacgctgt tcccatcttt aatggcttat agagggcacc
     5281 tttagctgag aataagacaa aacagaccct ctagcttcaa ttaagagcta gagggtctgt
     5341 ttttgatttt aaaacttggt ttatttcttc aaatggtagc tataggtgct gacaacgata
     5401 ttttcacgcc aagacaatcg caatcttaac cgcaaacttg tttggttatg gagaatattc
     5461 tgccagcgtc tgtttggtac aaattgagga atcaacacag tagttgtata gttttgtttc
     5521 gctgcatttt tacttacgat atctacataa cggataattg gattttcaat cgagcgataa
     5581 gaagagtgga cgattgaaaa acggacatct gggaaatgct ttttaaattc tgcttcgatt
     5641 tctttttctt tttcgacatc ttcgtctagc gaaacatgca tcgcaacgac ataatcaccg
     5701 atcgaacgtg cgtaattcaa cgcaccgata tttacccgtg tcacattccc gactaataca
     5761 agaacggtat tcccgtcata atcatgtagt tttgcatcat tttctaaacg aagctgttct
     5821 gctacttttt tataatgatc atggatctta taaaaaatga acataacgat tggcataatg
     5881 atgaagaaag gccaaatgtc ccctaaccga tagacaaaca aaatcgcaat gattgcatat
     5941 gagatgaacg cacccgtaat attggcaatc gatttgctta accatctttt tccttctttt
     6001 ttccatttga cgaccatccc tgtttgagat aaagcaaatg gaatgaacac gccgatagag
     6061 tataacggaa tcaagcgttc tgttgagcca tggaaaataa acagcaatac gatcgaacct
     6121 agcgctagtg tgataatccc attggaatag cctagtcgat ccccacgatc ttgatacatg
     6181 tgaggcatga atttatcttt tgccagattg tatgcaagaa ctggaaaagc agaaaaaccg
     6241 gtgttagcag caactgctaa aatcagcgct gtagcaaatt gaaggacgta atacaaaata
     6301 ccatgtccaa agacagcttt accaatttga gacaaaacag tgacttcttt ttcaggaacg
     6361 atcccatacc aatagttgat aaatgtgatc cccacgaaga agaaacctaa gatcaatgcc
     6421 atcatcgtca atgtagcagc ggcatttttt gctcttggct tcttaaagaa tggtacagca
     6481 ttactaatcg cctccacccc agttaaagag gaagaaccag aagaaaatgc tcgtaaaatc
     6541 aaagcgatag aaacacccgg cacagccgct cctggaagtg cagtagcttg caatggcaca
     6601 gcacctgtca cgatattata taaaccaaca acgatcagta acgagatcac aaggataaat
     6661 gtgtacaccg gaaataatag gaacgatgca gattccctca gcccacgaag attcagcatc
     6721 atcagtagca aaacgattgt cacagaaata gcgacttgat gaccgtagag tgctggcacc
     6781 gctgatgtga tcgcttcagc acctgctgag acagatactg ctactgtcaa catgtagtcg
     6841 atcaataaag agccaccgga aatcaagcca gcattctttc caagattctc actgctgacg
     6901 acatatgctc ccccgccatg aggataagca tggatgattt gacgatatga tagcgttaat
     6961 gaaatcaata aaataataac aaaagcggca atgggtagcg aataccagat ggctgcagca
     7021 gacaaagcga ctaaaacgac aacgatttgt tccgtaccat atgcaataga tgataacgca
     7081 tcagacgaca ataatgccaa agcagcaaat cgggttaatt tgtgctcatc attttcagca
     7141 gattttaaag gcttacctac taacaacctt tttaagtaat ccaatttcta aactccttta
     7201 cacacaattt tacttcaagt tttatagtcg aaaatttata gtaaacaaga tgttatagta
     7261 cgctcactga ccttaaaagt caataaactt taagaaaata attggaaaaa aacagaaaaa
     7321 tatcaagcgc tttttcaaac gctttaatac agcaaaaatc taacattttt ttatgggctt
     7381 tttctttgaa ttctataata aaaggataga acttgactta gaaaatcaag ttctatcctt
     7441 tttgatatct taacttttgg aagagagatg taaggctgct ttctacccat cattcttact
     7501 actcgaagca tagcgcagtt gtcacaacct cttgataaac ggtgtttaaa agcttgcatc
     7561 tgcggtaata agctcaacca aattaaaaac ataagaagct gtttttattt ggtcgttctt
     7621 attacttgat gcagagcagc ttttttacaa cctcttggta cacggtgttc aatcagctga
     7681 ccctcggtat tagctcaaaa aaacggcaaa atatgaggaa ctatttttcc atttttttct
     7741 ctaatactca ggtcaaaaca ctgatttcac aacctctttt tttaatgaaa aatatggctc
     7801 tcatgcgccg cttctttgaa tttcgtcatt ttttcttcga tgaacggata gattgccgtt
     7861 cctacgattc caaagacaac aaacaaaccg atcatgatcc agattgcttt cgtagcattt
     7921 ggccaataaa ttcctcctgc agattcccgt aacaagttta ccgcatgagt aaatggcaag
     7981 aacggattga tcatttggaa gaatttccct gagacttgga tcggatagtt cccgcctccg
     8041 ccagagatcg acagtactaa gatgataata gcgatccctt taccgacatt atcgaataat
     8101 gcagccaaga catagacgat catcatgaac gctaaggcga tcaagacagc aaatgacacg
     8161 ctgtagaccg gattttggac atctactcct agtaagaaaa tattccctaa tgtaacgatc
     8221 aagctttgcg caatcgccat tgtcaaaaat gtcagcatac gagcaacgaa ttgttctcgt
     8281 ttcgtaaaat tgtttcggtc tttctttcct aaatagtatt ctgtcgtagc aacacttgaa
     8341 agaagaacag cgccgaccca cagacatagt gcagtataaa acggggtgct agctgaaccg
     8401 ttatttgcga ttggataaag cgcattggtt tgtagctcaa caggttgtgt aaagaagtca
     8461 ctttcttttg ttgcgtctag tttcaataat ttgatgactt cgcctagatc gacttcttgt
     8521 tcgccttttc ggatcgcgtc tgctgctttt tggataccag ctttgatatt tggccagtca
     8581 ttgttgatca agtccgcagc agcgttcaca gctgtttcga cttctggcat tttttcattt
     8641 actgttgtca atgtaccggt gatttcattt tttagtgctg gccaatcatt ttggataaaa
     8701 tcagcggcca atcctaattt ttgctgaatg atcggtaatt cattgttgta taggtctgcc
     8761 ccttcattga tgccattgac gatggtatcc atatttccat tcagcattgt attggcatca
     8821 tggatttctt gtccaattgc aggcatttct ttttgatatt tttctaaaat tgccactgca
     8881 ttcgttacag ttgctttcgt tgagctgaga agcgaatcaa agtcgatctg gtccgctttg
     8941 ctcagtgcgc cttgtgcagt cccaatcgta tcgatgactt ggtctagtag agaagtgaca
     9001 gtggaactga tttgacctgt attgatacca gcaatgatgc cattgatatt attaacaacg
     9061 gcatcaattt gatctagcaa ttcatcaata gccttcgtgt cttcattttg aacagctgtg
     9121 ttcaaagcag ataggcggtt ttgcagattg cttagcaagg gttttaattg atttagctga
     9181 tcaataattg gctgtaattt cgtatttcca gcacttgttt gcaatttagt caggaactcg
     9241 accagtttat cgattgcatt ttgttgttga gatattccgt caataaagcc gttcaataca
     9301 ttgctgatgt ttttcttttc atcatcagtc agcttaccat cttcaatagc tgttcttaca
     9361 gtatctatta acgaacctgt cgttgttgcc acttgtccaa ttgacttcaa tgtaacatct
     9421 acactgtttg tgatgcttgg tatcgcatct ttcagctgag aggcacctga ttttgtttgg
     9481 tttgctagat cacttgcctg attttctaaa tttttcacat ctggcaagat atcttgtact
     9541 tgttggatga tcgtcaagcc ttgttttgct tcatcgattc cttggttcat tgtctcttgg
     9601 atcgaagcaa aatcgccgtc gatctctgct agttgcttac cggcattttg gatctctggg
     9661 attttttctt gcaaatcaag gatgattttt gcttgatctt tcaactcagg cattttgtca
     9721 ttcaatgcaa cgactttttc acccatctca tctactttag ggatgtaatc cacgaattca
     9781 tttgctttat tcagtttttc cttgatttca ggtagttgac tttgtaattc gaggacttgt
     9841 tttgtgtagc cgtcgatcgt gtccaaattt tcatctgttg ataaaatcat atctttaact
     9901 ttattgatac ttactaggtt cgtatcgatg tcgtaaccta tctcattgaa tactttcaat
     9961 aaagtcgaac tggctgtttt gataaattca tttgtgattt gcgattggat cgaacttgct
    10021 cctttatcgg tgattttcgg tgcaatcgca ttgatttttt catttaccgt atattcgatt
    10081 tttggtttct tgatatcacc actagtaaaa ctcagcagat cttctgagaa atcctttggc
    10141 aagtaaatac ctgcgtaata ctttccggaa cggactccgt cttctaattg ttcttttgaa
    10201 tcaacgaatt gccatcctaa ttgtttattt ttatgaagcg attcgatgac ttgctcccca
    10261 atggccactt ctttaccttg aaactctgct ggcttgtctg cactgtaaac agcaattggc
    10321 agctctccag tatttccata tggatcccat aatgctttga tgttaaacca cgcatatagc
    10381 gaagggatga tcatgatcgc gatgatcaga agtgttgcaa tcgggttttt gaaaatccgt
    10441 ttccagtcaa gcaaatataa tcgaagcgta tttttgattg actgcatagt tctccccctt
    10501 cttctttttt cagctgaatt actttaccat gtatttcata cttttttcaa tcggcttttt
    10561 tcaaaaaagt cgaatttttg gacatatcac ggcaattgtc tcattgtaat ctcttacata
    10621 ttgagaaata attaaggatt acagccttta ctttatcatg aaataaaagg gaaagataat
    10681 tttatgccaa agtttttatc tattatacgt atacgcaaaa aagctaaaac attttttgtc
    10741 tttgccccta caaaaatgtt tcagcttttt tcgtttttta ttcctgtatt tcttattttt
    10801 cgaatgacag gatgattttc ccagcaggag gatgtccttc aactagctga tgtgcttgga
    10861 tgacatgctt aagtgaagca gacagattca ttgaaataaa aacatgaaag gctccttttt
    10921 ggtaggcttt gattatgcca gcaaaggctt ctgcatctag atattccttt cttggaacaa
    10981 acgattcata aaagccttcc tttttttgcc gcaaatctag atctgggagg tcatttaatg
    11041 caacatacct gccgcctggt ttcatgatct ggatgccggt ttctcctttg atacttcctt
    11101 tcgtcgcatc gattacaagg tctgcctgat cagcaaattg cagacctgga tttgttttat
    11161 cataagcagc aaatgctgaa gctcctaatt tttttacctt ttcctcattt ttactggatg
    11221 cactggttaa gattcggatt cccttttcat ggagaagctg gatcagactt gacccgaccg
    11281 cacctgatgc tcctagtatc attacagtat ctgtcggctg gatttccgtc aaatggttga
    11341 ttagattata agccgtaatt cctggcgtga ccattccggc tgcttcttcc caactcattt
    11401 tatctggaat ttttgctact tttgctgatg gcaacacgac ttcttctccg taagtcccgc
    11461 cgactgcatg gacagctact cgatcaccta catgaacagt tgtgacatct gaaccaatct
    11521 ctgtcacaat accagcgcca tcattacctg gtacataagg gaatttcaat gttcgtatct
    11581 ctttcatttc tcctaaacgc aaggcaacat cataaggatt cacactgaat gcttttattt
    11641 ctacacgaac atgtccagaa ccgacttctc tgggatgagc atcgatttct tcaaaaacat
    11701 cttgtgcttc tccgtaaccg ttaattgcaa atcttcgcat ggtcaccatc tccctttttc
    11761 tttaagttta cgtttatgtc tttattttga caaaaaaaat gtcttttcgt tttggcgcat
    11821 gattcaaaac aaaatagttg tcttttttcg cgtaacgtgt taaattattt atctaaaagc
    11881 gtaaagatta cgatttacgc taataaaaaa taattggagg aatttgttga tggctaaaaa
    11941 atcaaagatt gccaaagcaa aaaaacagca agaaacgatt gaacgctatg ctgctttacg
    12001 ccaggaatta aaagcgcaaa aagattacga aggcttgcgt aaacttcctt tagatgctcg
    12061 tccagaacga ttgaaaaatc gtgatttaat cgatggacgc cctcgcggat acatgcgtaa
    12121 attcgggatg tcccgtataa aattccgtga attagcacat aaaggtttga ttccaggagt
    12181 aaaaaaagcc agctggtaat ccaagtctgg ctgtaatgta aaggaggagg gcgcatggac
    12241 cactccttgg atcatcaaaa aagatacgtt cgcttcttct agcagaaatg ctggtactag
    12301 aagcagacgt atcttttttt acgcacagct ggtccttttt tatatgtaaa ggctgcatgg
    12361 ttacttcgct atatttattt agtaacagga gtcagtgctt cgatacccac actaattttc
    12421 aatgctcggt caacttctgc cataatgggt cgatccaaat ggcagacctt ttctttcaat
    12481 cttgatttat cgatcgttct gatctgctcc aacaggataa cggagtctct ttctattcct
    12541 gttccagtag ctttgatccc aatgtgagtc ggaagcttag gtttagccat ttttgccgtt
    12601 atggccgcaa caatgatcgt cgggctaaaa tggttaccta gattattttg aataaccaaa
    12661 actgggcgtg ttccgccttg ctccgatccc acgactggag aaagatcagc aaaataaata
    12721 tctcctcttt ttaccatagg atcctcctta ttcttgatat tctcttggta tccgctgtcc
    12781 taatccgcaa gccacttcat agtggatcgt acctaattga tctgctatat cctgcatagt
    12841 gatttctttg tctgcatttt tgccgataag cgtcactttt gttccgacag gaaattcttg
    12901 aggcaatcgg atcatcagtt gatccataca tacacgccca atcgtttcac agtagttccc
    12961 ttctaccaat agagaaaaac cctgcatctt tcgtggccag ccatctgcat aaccgatcgg
    13021 tatcgttccg atccactctg cttcaggagt gatatatgtt tctccatagc cgattccttc
    13081 acctgctggg agttttttga cttgaatcaa ttcagaaacc agctcaagtg ctggctgcaa
    13141 tggataaact tccggtagtg cgtgcccaga gggattaagt ccgtacattg ctactccata
    13201 acgaatcata ttgccgattg tttcatcatg ccataaagcc gttgcactat tgctgacatg
    13261 tacatatcga ggaagttcgg gaagtttttt cagcacttct ataaagcgtt ctttttgcag
    13321 attccaatat gtatcatctg cctgatctgc tgtagaaaaa tgggtaaata aaccttccca
    13381 ttctaataca cgactttctt tgatgaatgc agctgcctct tttacttctt caggtgtaca
    13441 aaatccgata cgacccatcc cagtgtctgc ttttagatga atcgaaagag gcgtctctag
    13501 cttacttttc tctaaaacag cagctgctgc ttgcaaccat tcccgtgtac ctgcagttac
    13561 tgataaatca tgagcaacag ctacagtcag tgcgtcgtaa ggaaccatat tcaagataag
    13621 gatgggctga gtaaatccag cttctctcaa ctcgactcct tcatctaaat ttgaaacaca
    13681 aaagcccgaa gctccacctg caactgctgc ctcagctgtt tcaatagctc catgaccgta
    13741 tccgttcgct tttactactg caaacaattc ttttccttgc ggaagtcttg caacttcatt
    13801 tgctacattt tcagttatag cttttttatg tatcactgct ttcgttgggc ggtgccaagc
    13861 gacaaccatc gtttttcctt ctttctagtc ttccaaaata atttgtgcaa aagctgtttc
    13921 atttgtatga gtaatactga cccatacatt gccttcatga ggtgaacgcg tcacaacagg
    13981 ctgaccattt tcatttttta aaatctcgac atcttgaaag gtaacttttc cgatcccagt
    14041 tccccaagct ttagagaaag cctctttgca agcgtaacgc ccgcctaaaa attctacttg
    14101 gcgatgaaaa ggcaaggact ggaatagttt catttcatca gaggtcaaga ttcgtgcaat
    14161 gaattttggt ttttcttcga tcagccgagt gatcctagga agttcgactg catcgatccc
    14221 aattcctttt atcatatgct tttctctttt caataaattt agaacactct cattctacca
    14281 aattataacc attttgccac aaataagtcc acaaataaca aaaatgaaag tttctcttaa
    14341 aaaagagaaa ctttcatttt atgtttaatc gttatttgaa cggattacaa atccgcgttt
    14401 tttatcattg tgacgtttac cgccatcttt ttggctgtta ttatttttgc tataaccgcc
    14461 ttttgatttg tttccacgat aattaccgcc gtcacgacga ttacggttgt ttccgccacc
    14521 acgtttgcca tttttattgt atcctttttt ctgcattggc aatgggcgtt ctggtgtgat
    14581 cttcactggc actgcatcag ctggatcttt tgctgtcgtt tttagcaaaa gtgcaactaa
    14641 atcttgtgca gaatattctt ctaataattt atctgctgtt tgcaggtatt tatctagacc
    14701 attttctgca agttttgttt cgatttgttc aactgcagca cctaattgac ctttgaatgc
    14761 ttctttttcg gttggtggac gtaaagtcgt catccgtttt ttcgtcaagt tttcaatcac
    14821 atgcaagtag tccatttcat ttggtgtaac gaaagtcaca gacataccgc cttttccggc
    14881 acggcctgta cgtccgatac ggtgaacgta gctttctgga tcttgaggga tatcatagtt
    14941 gtacacatga gtgacacctg aaatatctaa tccacgtgca gcaacgtctg tcgctacaag
    15001 gatatctaaa tgaccgctct taaatgaacg caatacgctc attcgttttt gttgagacaa
    15061 atcgccatgg attccttctg cacggtaacc gcgtgcttct aatccgcgtg ccaattcgtc
    15121 tacacggcgt tttgtacgtc cgaagacgat tgttaattca ggtgtttgaa catcaaacaa
    15181 gcgagtcatg atatcgaatt tttcgtattc ttttgcgcgg acatagtatt gatcgatcaa
    15241 gtcagctgtc atttctttcg ctttgatctt cacatgatgt ggatttttca tgaatttaac
    15301 gccaatattc ttgatagctg gcggcatcgt tgctgagaaa agtaatgttt gtctttgatc
    15361 aggaacttgt gaaatgattt tttcgatatc ttccaagaag cccatattca acatttcatc
    15421 tgcttcatcc aaaactaatg tttgaacggt tccaagtttc aatgtgtggc ggttaatgtg
    15481 gtcaagcata cgtcctggtg tacccacaac gatatgtgga cgatctttca agccacggat
    15541 ctgacggcca atatctgctc cgccgtaaac ggcttgtaca cgtacttttt tgtcgcgccc
    15601 taaacgatat aattcttctt gtgtttggat agctaattca cgtgttggtg cgataactaa
    15661 accttgtaat tcgtgacgat ctggatcgat tttttctaac attggtaatc caaatgcagc
    15721 tgttttccct gtacctgtct gtgcttgtcc aataacgtct tttccagcta atgctagtgg
    15781 aatcgtttct gcttggatag gcgttgcttc ttcaaaacca gcgcgttcga ctgatttcaa
    15841 caattctggt gataattcta gttctttaaa tttcaaatga atcctcctat atggttgtta
    15901 actgaggatg tagctttttt gctaccccgt cgatttgttc ggtcaaagtg tattgtttct
    15961 ttttgacagt ggccacgcac gtttttatcc attcttagcc cctgtggcac caccgaaaaa
    16021 tcactttgcg aagtttaggc attagaaaag ccaaacgggt cgcgttcagc tttatcatta
    16081 ttatgacatc tggcttcaac aagttgtttc tatggcaaaa ccagtccaat ttctcttcat
    16141 ctttcggcaa taggttattc taacatagtt tttcgttttc tgcaagttta cagattttta
    16201 tttcatttgc tgaagttgtt ctaccacttc aaataagccc atgctgttgc tgccttttag
    16261 cataatacta tctgttgctg caagattttg tttgatagca tcaatcaatt tattcttttc
    16321 tttttcaaaa tagaacacag gtagttgggg gtatttttgg ttcaattctt cttttagtgc
    16381 atgcatttgt tccccataaa ggaaaatcat ggcgaatttc tcaggatcga tatgttcact
    16441 catttgcgca tgcatattta aagactcttt ccctaattcc agcatatctg ctaacacagc
    16501 gattctgcgt ccttttgttg gcagattttt aaaagtatct aataccagtc ccatggctgt
    16561 tggatttgca ttgtagacat cgctcaaaat agctgcacca tttcctgccg ttagccattc
    16621 ggttctgttt tgagtgatag aaacatgggc gagcccttgg cgtatctcag gtatggacag
    16681 tccaaagaag cgcccgatcg tatacgcaat caaagcattc gttacattat aacttccagg
    16741 cagcgggatc gtaaatgttt ctccttctac tacaaaatcg gtttgtgctt ttccttttgc
    16801 tgtcacttcc gcatgaacat cgccatttcc aataccgaat gttgtgactg tttgtactaa
    16861 tgttgccgtt aaaggtgtca gcaacggttc atctgcgggc acaacgagta atccgtctga
    16921 tacaagtccg tcagtgattt ccattttggc tttagcgatt ccttctcgcg acccaagatt
    16981 ttctatgtgt gcttcaccga tcattgtgat tgctgcagct tttggttgag ccataagtga
    17041 aagttccgtg atttcattgg cgtgatccat gcccatttcc aaaacgatct tttctgtatc
    17101 ttggggcatg tgcaaaatcg tgtaaggaag accaatatta ttgttgtaat ttccttgcgt
    17161 cttatatgtt ttgtattttt cagacaagac agacgcggtt agatctttcg tggtggtttt
    17221 accattgctt cctgtaattg caacgacttc tgcgcctact ttttccaagt aataggttgc
    17281 caattgctgc atggctttca aaggatctgc tacaggaata actggaaaat tttttggagc
    17341 ttgttcaaca ggcaagctcc aaaatgaagc agatgctccg tttgtgatcg cttgctggat
    17401 aaattcatgt ccatctcgcg taccagcaag aggaacgaat aaacttcctt cggttatctt
    17461 ccggctgtca aattcgacat tggtaatctt tgtcagtgca gagcattcgc tgcccacggc
    17521 ttgtgcaatt tcttgtattg ttagattcat tgagatctct cctaaaaaag gttgtgaaaa
    17581 aagcgtttgg cctaaacggc gggttcgcat gactctcact tccaagtaaa tttctctatt
    17641 cgtatttaga agctagaatg aacgcggtta cgcaatcact ccagcctctt ctttatttct
    17701 ctttattcat ttttttcaaa aaaagattga cgctgatggt atcgattcat tcctaactgg
    17761 atcagttctt caatcaaatc accgtatttc aagcccatat tttcccataa gagtgggtac
    17821 atactgaact ccgtaaatcc tggcatagag tttaattcat tcaggaataa ttcattttta
    17881 tttgtcaaaa agaaatcgca ccggctcaat ccgcttccac ctaacatcgt gtaagctaac
    17941 ttcgcgtact cttgcgcttt ttgataaact tcttctggca cttcggctgg aatctgcatt
    18001 tcgattttat tattgatata ttttgcttca taatcataga atgctacgtc ttttacgact
    18061 tcaccaggca aagtcgtccg aacgtcttca tttcctaata cagcaacttc gatttcgcgc
    18121 gcttcaattc cttgttcaac gattgctcga gaatcatact gataggctgt tgctaaagca
    18181 ttttgcagct cttctcggtt ttctgctttt gtaatgccga cactagaacc catattcgcc
    18241 ggtttgacaa acatcggata aagcaaagaa ccttcacatt gatcaaatac ttttttagga
    18301 ttttctttcc attgattctt aagtactggt acataaggaa cttgcggcac accagcagct
    18361 tgtaaaatat acttggtcat gattttatcc atggcacatg cactggtcaa tacgcctgcg
    18421 ccgacataag gcatattcaa tgtctctaag aagccttgga tcgttccatc ttccccgttt
    18481 ggcccatgta aaactggaaa aacgatggct ccttcttctt tgatttcgcc cggattgatc
    18541 acttttcctg taaagccttc ctctgtctgt ccacttgggt cccatgaaag atgcaagaca
    18601 tctttgctgg caggtttttc tgttagtaat ggacctttga cccattgtcc ttcttttgta
    18661 ataaatacga gttgaacttg gtaataatta taataaatgg catttaaaac tgaaaatgcg
    18721 gaaagaatgg acacttcatg ctctgcgctg cgtccgccat atagtaaagt aatcttcaaa
    18781 agattgtcct cctgtattct aaatcgttca gtgatttact ctcatatttt agcacaaaac
    18841 aaaaataaac gaataactta tttataaaaa aacgagccag cttctaaaag aacgagcgtc
    18901 atttattttt tgtcagctag attatttgtc ccgtttttta taaaaagtta tgataaagaa
    18961 aaggagggat gatgatggcg caaacaaatc cacgcagccg cttggcagaa atcattcgag
    19021 tgttcatcaa ttacaatgta gtgccaaact ttgtacagca aaaaaatccc gaacaggtaa
    19081 aaaaagcctt tgaagaacta ggccccacct tcatcaagat aggacagatg ctttctgttc
    19141 gagaagattt gctttcttct gccttcactc aaacattcaa aacattacaa gatagtgtac
    19201 ctagcgacac attttcaacg gtcaaaaaga cgatcgaaac agaactttct ctttcattat
    19261 cagatatatt cgatgatttt tcaaaatcgc cttttgcctc tgcttctatg gggcaggctc
    19321 atcgtgccaa gctgaagaac ggtgacgcgg tagtcgttaa gatccaacac cctaacatcg
    19381 ccgaagagat ccgacttgat cttcaactat ttgaacgagc gatccctttg atcaaataca
    19441 ttcctgaaac tagcgtagtt gatctaaaag gggtactcca agaagtaaaa cgttccttga
    19501 tcaatgaaat ggacttctta aaagaaagtc aaaatggcga acaattctat caaaagaaca
    19561 atggctggaa agagatccgc agccccaaga tttatgacgc tttttgttcg aaaaaagtga
    19621 tcgtgatgga agaaatgtct ggtaaaaact tgaatcattt aatgaacgcc gaaaacaaaa
    19681 ctgaaacttt tatcactgga attcaaaaca agcagctgaa acaagaagtt gcaaagcttt
    19741 tagttgagaa ctttatgaaa caggtgtttg atgatggttt tttccatgct gatcctcatc
    19801 cagggaatct gctattccat gtactaacaa aagaagaaca gacacaagct agccgaaaaa
    19861 ctgaaacagt ccatgaaaaa gagttcggtt cctttgcttt tcgagcctcc acttctgcag
    19921 aagaccctgt tgcgccatat accatcaact acatcgattt tggtatgatg gggcatcttt
    19981 ctgcaggact taggcaaaaa ctcacacaag ccgttttagc gctgtatacg aaagatgctt
    20041 atcgaatcga aaaagcggtc cttcgacttt gtcagcaaga aggctcgttt gatgaaagtc
    20101 ggttccacca agaactgaca agttttcttg aacagtatta tgattctcct attgatgaaa
    20161 tcaacttgca agaagtattc acacatgtcg tgacgatttg tcatcaaaat aatctgcagt
    20221 tcgatcgtga tatcacgctt ttgctgaaag catttgggac attagaaggg gtgatccgcg
    20281 tcctcgatcc tgaagtttcc ctcatggaag tcgcttcgcc ttttgctcaa cattattttc
    20341 tcactcatct ggatgtagaa gatacattga aacaaagcgg attagactta ttggaaggga
    20401 tgaaggctgc gcctaagatc cctcaacagc tccaccatct attagaaatg tggacttcgg
    20461 gacaaggaaa agtcaatttg gaattgaaaa agcaagacaa attattgagc aggatcgaaa
    20521 gtatgatcaa tcggcttgtt ttcggtatga ttttagctgc attgatcgta gggtcctccc
    20581 tgcttgtcca agcagcacct gtagaaaacg cagaggtcgt ttccttatta gggatattta
    20641 cgtatgctat tgccgcattc gtcattattt ttttggcaat cgatgcattg atccaaatgt
    20701 ataagaagag gaaaaagtag aaaaaggttg tgacagaaag aatcagcttc aagaaataag
    20761 aaggggtttt acataaattg tttctcaaat ttttgtgaaa attcggctta tttttcgaag
    20821 aggctacttc tgaaacaccg agaataaaaa atgtgaaaaa gctgttctgc acccagtaat
    20881 aagaacaatc aaaaagggaa catggaacac atttatcctg tttccctgtt ccctttttga
    20941 tgtattcgag cataaataaa ataccttcgt tacctactat cttctgtact tggtaaacct
    21001 tagtcaattg ccttttttaa cgcttgacga aaagacatat gatctttctc cgtaaagggt
    21061 tttggaccct ttgtccttat gccagctttt cttaactttc cactatgata acgttgttct
    21121 agaagcgtgc cgatattcgc ttcgtcaaat tcttttccag agtgatggaa cactcgcaca
    21181 cccttcggca atacaagtga agctaaacca taatcttgcg tgatcaaaaa gtcattcttc
    21241 ttaaccatac cgacgatttt ataatccgca ctatcagctc cgcggtctac gtagacgatt
    21301 tggacatgat tagcgtattc tttggtcgta taatggtcca cactggtcac gataaccacg
    21361 tcaagaccat aggattctcc taattttatg acatcttctt ttactggaga accatctcca
    21421 tcaatgaata gcctcattct ttttcgcctt cttccataaa tgataaacag ttactaatcc
    21481 gctcagtaaa agaccaacat tgatccattt tgccaagata agcagttgta cactttctat
    21541 cggcactgat tcatggttca gcgtgatcaa taatagatgg attggctctc ccatttcttt
    21601 tgctaaaaca aacaaaagcg tcatcaataa aaactggtgg atcacactcc ctttattttg
    21661 agtagtatac ttgacaacga tccggcgatc cattggaata gctgtatcag tttgataata
    21721 ttccttattt agccagctgc ctgttactac gtgccagaaa ctaagcagaa agacacctat
    21781 cgcaaaacca tttggaatca agaggaccaa taaagaagaa ctcagtccaa gcaattgcat
    21841 agccaatgga gaccatgtta aacgtttcaa aatccatggt ccgactattt ttgctgcaat
    21901 caagcctgct gcataaggca aatacataaa aatcgaaagt ttttctaatc cgtacagtat
    21961 gccaacataa aaactgctaa ataaaaaaag gaaattccct agcattccat ttagaaaagt
    22021 aaacagattt agccagcttg gcaacttcca ttccttcttc tggtgtccta aaacaaacac
    22081 gaacaagatg aacagcagca agaaaccata aatagcaaga tcaaaaagcc tttcatcgaa
    22141 taacaatcgg gcatttctca aaagaaacaa taagccaaag aatatcaaaa ataaaacaag
    22201 ctctcgcgta gctaccagat ttcttttgat gtctttgaaa tccagttcgt aacgcggata
    22261 atgactaacc gtatggtagg ccatgacgta aaaaagtgta taaatagtaa agcttgctgc
    22321 aatctgagtc ggtgcaggca actcaatcgc ttgatacagg agaaatagcc agatagctgc
    22381 aaataagtac tttttcccat ccatattgat aaaaccttgc gtcttttcgt gaaaattgac
    22441 cgtggtatta gcaggtacaa gccatgctgc actcagccct agcaaaacac ttgaaaaata
    22501 aaataaagga aaataaaata cgcctaatat tccaagcaat gaaccagcac caccaatcag
    22561 cagagcgatc atcagcaacg tgtaactatc tagaccaaat cgtagactgt tgactaaaaa
    22621 tagtccagtc atccgaaagg cataaaacag cgtaaatggc aaaatcgtca gccaattatg
    22681 cgagtctgtt agtcccaaaa ataaaagata aggaataaaa ataaccgtgt tacttaagat
    22741 aaagggcgtt gtttgtgtaa aattacgccc aaatcgtttg agtttttcga acatgacaat
    22801 ggctccttaa acagtcttat aactaatctg ctcttcgatt ttagcattaa aggggaaata
    22861 aaccaatttt acagaatata ttcttgtaaa aaaaaataca gaaagaagca taagcttctt
    22921 tctgcatttt tacggtactt gattactcaa gcagacaagc ccttctccac aaaagtcttg
    22981 cccgacaccc ctgcatccgg caggtgataa agatagaaat caattatcct gtgcctccaa
    23041 aatcaattag gagctctgta aaccagagtc cttctcgacc cgtcgcataa atttacttta
    23101 acacagttcc tatgtttatg cgagagcttt tttcatttta taatcgacca tttgaaatcc
    23161 ttgaccgata tctgaaataa gttcttctgt aatctcaaat ccttcatgct tataaacctc
    23221 gattgcttga tgattcccct gattcactcg caaaaagatc tctctttcct ttgtttgcgc
    23281 agcaagttct tcataccagc gaaaaactga agaagtcaat ccttgaccac gtactttatt
    23341 tagcaaatat aatttactta aataaatttt tccatttatc tcttcataag cagtgtaacc
    23401 gacagtcttt tcttcgcttt ttaataaaaa gtaggacaca ccttctgtca gttctttttg
    23461 aatctgtttt tttgactgat aagttttcag catgtaatcc acttgttttt gaccaatgat
    23521 cggcacaaaa gcttccggcc atacttcttg tatgactgcc atcagctcat ccagttcatt
    23581 ttctgttact tttatttttt ccatacaccc acgtcctttt ttacattata aatgaccttg
    23641 cgcttgatgg aataaaaaga gcatgatcat gctatacaca cccaccgacc atggtttcaa
    23701 gaaaatcaca atctgcataa attccctaaa agataatttc gtcaaaccag ctactagaca
    23761 gatcaagtcg tctggcgcaa agggaagcag caaggtaaca actagtactt ttttaatatc
    23821 tttcgttttt cttgtcagca attcatcaaa tttctgatac ttttttggcg ataaaataag
    23881 ctggacaaag gctacgccat agtaacggac aagcaaaaat ccgattgctt ccccaatcac
    23941 taggccaata caactatata aaagcccaaa tccattgcca aacatcagca tgcccgctac
    24001 tgaggagatc cctcccggca aaataggaat gatcacttgg atcacttgca acagaacaaa
    24061 tatcatcact gcatacttgc caaattgctg gataaacgct tgcaaagacg tgctcgatcg
    24121 aaaaatccca tgatgataag cataaagaat caacacaaag aaaaaaaaca gaccaataat
    24181 gggcatgatc tgaagacatt tttgtaaaac tgatcctttt ttagacatct atattcccca
    24241 catctttttt catttattaa aactgcctc
//
LOCUS       DAMEET010000040        24037 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.040, whole genome shotgun sequence.
ACCESSION   DAMEET010000040 DAMEET010000000
VERSION     DAMEET010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 24037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.040"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            158..3436
                     /locus_tag="PB780_001419"
     CDS             158..3436
                     /locus_tag="PB780_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HDE0733480.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3977..5884)
                     /locus_tag="PB780_001420"
     CDS             complement(3977..5884)
                     /locus_tag="PB780_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HDE0733481.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            6014..6889
                     /locus_tag="PB780_001421"
     CDS             6014..6889
                     /locus_tag="PB780_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HDE0733482.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6969..7943
                     /locus_tag="PB780_001422"
     CDS             6969..7943
                     /locus_tag="PB780_001422"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDE0733483.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8123..9268
                     /locus_tag="PB780_001423"
     CDS             8123..9268
                     /locus_tag="PB780_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDE0733484.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9405..9749
                     /locus_tag="PB780_001424"
     CDS             9405..9749
                     /locus_tag="PB780_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HDE0733485.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            10020..11306
                     /gene="efmA"
                     /locus_tag="PB780_001425"
     CDS             10020..11306
                     /gene="efmA"
                     /locus_tag="PB780_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HDE0733486.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11444..12043)
                     /locus_tag="PB780_001426"
     CDS             complement(11444..12043)
                     /locus_tag="PB780_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HDE0733487.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12447..13568
                     /gene="mnmA"
                     /locus_tag="PB780_001427"
     CDS             12447..13568
                     /gene="mnmA"
                     /locus_tag="PB780_001427"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDE0733488.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13814..15235
                     /gene="arcD"
                     /locus_tag="PB780_001428"
     CDS             13814..15235
                     /gene="arcD"
                     /locus_tag="PB780_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HDE0733489.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15249..16367
                     /locus_tag="PB780_001429"
     CDS             15249..16367
                     /locus_tag="PB780_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HDE0733490.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16427..17719)
                     /locus_tag="PB780_001430"
     CDS             complement(16427..17719)
                     /locus_tag="PB780_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDE0733491.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17851..18429
                     /locus_tag="PB780_001431"
     CDS             17851..18429
                     /locus_tag="PB780_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDE0733492.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18558..18935
                     /locus_tag="PB780_001432"
     CDS             18558..18935
                     /locus_tag="PB780_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HDE0733493.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18868..20940
                     /locus_tag="PB780_001433"
     CDS             18868..20940
                     /locus_tag="PB780_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDE0733494.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21151..22098)
                     /locus_tag="PB780_001434"
     CDS             complement(21151..22098)
                     /locus_tag="PB780_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDE0733495.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22344..22808
                     /locus_tag="PB780_001435"
     CDS             22344..22808
                     /locus_tag="PB780_001435"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDE0733496.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22870..23913)
                     /locus_tag="PB780_001436"
     CDS             complement(22870..23913)
                     /locus_tag="PB780_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HDE0733497.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT         7808 a         4404 c         5055 g         6770 t
ORIGIN      
        1 ttttccgtta ataaatctct ttattatcaa caacttaagt tatatttttt ctttttataa
       61 aatacactta ttataatttt gttttttatt gacagtaaaa ataaattagg taatacttta
      121 agtaaacgct ttctaatttg ttacgggagg aatacacttg ttaaaaaaac gtataaaata
      181 cagtgcgcta atgatgaatc tccttatgct cagtaccccg gttttaattt ctgtcgttga
      241 agttgctgct aacgaacaac agtctgtgaa cgaagaaaat gcacacgtga acattgaagg
      301 gatgtcaaca atggaggaaa taccaagaac tagtgaagat atggatacgg aagaaggaaa
      361 tgatcctatg atgaaagaat cagaaaatga aaagacaaaa aagcaatctg aagaagcaaa
      421 tgaagagtgg aaaggggttg ttttcggaga aagcacaagt gctagtaggt atgccattga
      481 accattagaa gacggcgtgc ggctctcttc cacgaataac ggcggaaaat tccagtcaag
      541 cggatcagat ggaatgacct attattatca agcaatcccc aaagacatca actttaggat
      601 gcgggcgacg atcgaaatag aatcttggac ctatacgaat gcccaagaag gatttgcttt
      661 aatggtaaga gacgcagtac caaaaaatca tttgtttggg caggcatttt attcaaattc
      721 atttgccata ctaggcagtc ggatcgaata tgtctgggac tcggatagac aagaagtggt
      781 caacacgagc ggcagcaaat atacgatgcg acttggattg gggacacggg caatcacagg
      841 aatatcatct gaagatccgc aagctgcgcc agctcctggg agtgtctcag tggaaacaac
      901 acctttagag acttcagcag ggttcttgga aaaagaaaca ggttcttata atatattcgg
      961 aaacggtcat gggaatctgt ttccggccac gaactcgatt actaaactgg acgtagagat
     1021 gaaaaaaacg aatactggtt tagaggcata ttattacgat cctgaaacag gagaagaaat
     1081 ggcttctgac atcatgtatg actgggaaaa attatacgct tcagatgaat cggttattta
     1141 tgcaggattt gctgcagcaa ggaatatgac gatcaaggta gaaggaatcg aagttgctta
     1201 ctcagatcct gcgacagatc ctcctgggca agaaagacct actcgcttga ttgatccgat
     1261 ttacactgtg acctctagta atcattcagg aaaccaagat catactatgt cctttagagc
     1321 aaatgctgac ggattattga caattaaaaa taaagcaaca ggagaggtgc ttaagaacgc
     1381 aaaaaacttg gctatcactg cgaatcggga atttgattat cagacaaaac tgatggaagg
     1441 aacgaacgaa tttactttca gttttacgcc agataaaaac tatccgccag aagaatacgt
     1501 agagatgact agttatgaaa caaaagagat tcttcataca gtggactacc gtaaacctaa
     1561 atattcgaca gtttatgtaa caccagaagg gtcagatgca ggaaacggaa gcagacaaaa
     1621 tccattatct ctgacacaag cattgagata cgcatatgct gggcagacaa tcgtcatggc
     1681 gcctggaact tattccttcg aaagaggatt gacgattcca aaaggcgtta acgggacaag
     1741 agaaaacccg attcagttga ttgcagagaa aaatccagaa aacaagcgtc ctgtacttga
     1801 ttttaaagga actggaaatg ggatgacact gttcagtcac tactggggtt tacgagggtt
     1861 tgacattaca aatagtgcag ctatgcaaaa aggattacag atttctggaa atcataattt
     1921 gatcgaagag attcatgctt atcggaatgg aaatacagga atccaaatca gcggtagctc
     1981 aaatgatcct ttcgaagcat ggcccgcgca taacttagtc aagaactgta catcttttga
     2041 aaatgccgat ccaggatttg aagatgccga tggatttgcg gccaaactga caataggaga
     2101 aggaaatgta tttgacggtt gtatcgcgta tcataatgca gatgacggtt gggacctttt
     2161 tgctaaaaat gaaacaggtt cgatcggaaa agtaatcatc aaaaactcag ttgcttatcg
     2221 aaacggctgg gtaccaggaa ttgagggaga aggtaacggt aatggattca aaatgggcgg
     2281 ttcttcttta acgggccctc atgaattgat caatagtttg tctttcgaaa atcttgcgaa
     2341 aggaatcgat tccaatagcg gaacagatat tatcgtcaat agcagtacaa gttttaataa
     2401 cggttcatat aatgtggctc tttatacctc ttcagcagga aacacagact accatgcttc
     2461 tggtatctta tctttccgaa cagagaatct tgagatgaga gagcagatcc gcccattgaa
     2521 tcaaaacgaa gatcaaatct accactcgtt gaactactat tggaatgaac gagagaaaca
     2581 atcagaaaat actcttggac ataccatatc aaaagattgg atcgagtctc taaatacatg
     2641 ttcgaaagaa ggtcagcagc ctgttacgcg ctatgaaaat ggaagtatcc atgttcatgg
     2701 actgatgcag attaaaccgg ggaatcgtca aacagaaaag gaaagagtgg atggtcttcc
     2761 attatctgta ggtgctattt ttgacggaga aacctcaccg tcttcggaat atccagagta
     2821 tgcaatcgta caagaaccaa atcagacaaa accaaatgaa caaaataatc cgattattat
     2881 acaagaagca aatggtgtgg cggagaatgc agaaggaaat atcattgaac tgaagccttt
     2941 tacagttttt gtgtcaactt tcacaccaaa tcaaggaacg cctgtctttg aagatatgcg
     3001 ttatttccca gaaaagatcc aaatcgttga aacacagtca agtgctcaat tttctaatca
     3061 taccgctatt ttttcagcac cttcaaaagg agaatatacg ctagaagtca cttatcgttc
     3121 ggaaatattt gatggggaaa aatggtctgc tcatacagaa tctgttaccc agcaaaaagc
     3181 gatcactgtg cgagaaactg ccgatgtgcc cccgggaaat gacaaaccag aaagagaaaa
     3241 tccaggaaat gaatcaccaa atggaaaacc aaatggaaaa ccttctcatc cggataggac
     3301 agcaaaagaa agtgaaaaaa atggcactca aggactccca aaaacgggag aatttaataa
     3361 cccgttgcta gcattagctg gaggaatttt gttaatcggt gtagttgttt atgtcatgaa
     3421 acagcgaaaa aaataagtgg aatttctcct tttatgatgt aaaacaattg actgaggaaa
     3481 gtaaaaatag aaggaaatga gaccaataga tgtctcactt ccttctatct ttttatagat
     3541 gaggttctcc ttgctgcttt tgattggctg ttcttattac ttgatgcagg acagcttttt
     3601 cacaatctct ccactaaacg gtgttcaatc agctgaccct cggtattagc tcaacaaacg
     3661 gcaaaatatg aggaactatt tttccgtttt tttctctaat actcaggtca ggacgctgat
     3721 ttcacaacct ctcgataaac ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta
     3781 tgcaaaaatg tggaaagcca ttttcacaaa atttttctta ctacttgaaa ctaagcagct
     3841 ttttcacaac ctctaataca tggtattcca gaagtaactt cttcgaaaaa taagccgaaa
     3901 tgtcacaaaa atttgagaag cgattttccc aaattccttc ttatttcttg aagctgacca
     3961 cttctggcac aacctcttac cagattcgca ctcttttttc tggcgctaga tacatagcgt
     4021 cttgttccgt gataccaaat gtttcataga attcgtcaag gttttttggt tgcacgtttg
     4081 ctcggagttt agcaggggca tggacatcga tttgcagtaa aagttgttgg tattcttttt
     4141 ttgcttttgt acgccaaatc gttgcccaat tgacaaagaa ttcctcgata gaaagatctg
     4201 attccgtttt tgctgcttcc aatgcacagc ttagaccgcc tgcatcagca atattttccg
     4261 aaacggttaa ttttccgttt acttttccgt cagcaaaagg cagaccatcg aattcttcga
     4321 tcattgcttg tgctttgttt tgaaattgtg taagatcttc ttctgtccac cagttgttca
     4381 agttgccata ttcgtcaaat aaagcgccgt tattatcaaa tgcatgggaa atctcatgag
     4441 cgataacagc tccgattcca ccataattag cagagcttga ttgttctaga ctgtaaaatg
     4501 gtgcttgaag gatcgctgcc ggaaagacga tcacattccg aaatggatga taataagcat
     4561 tgaccgtgtc agcactcatt tcccattctg aacgatccac tggtttgttc catttgctga
     4621 aacgatcttt taatgtgagt ttgctgaaaa caagagtatt tgaaagcaaa gtaccaccct
     4681 gatctgcagg tactgttttg aactggtcga ataatgctgg gatcttatcg gggtagccta
     4741 cttggatacc taagttattt aattttatga cggctttttc ttttgttgca tccccaagcc
     4801 agtcattttc ttctagacgt tttttataaa cggcgatcat ttttttcacc atttgttcta
     4861 cgtctttttt agcagcttcg ccaaaatatt tttttccgta gtaatcgcca actacttgat
     4921 cgaattgtcc agaggcaagg taatacgctg cttttttctg aggcattgct tcgtctgttc
     4981 cagacaatac gcgagaatag attccgctga cttggcggaa ttcttctgat agataaccac
     5041 taagagaacg tactgtttta atgatcatcc agcttttgaa taacggaaaa tgagcttctg
     5101 tcaggatgtc attcaaatga tcgaagtaca ctggatcagt tacgatcact ttatctggag
     5161 ttgtcccaat caaacccgtg acaagttttt tcaagtccag tttatctgtg tatttaataa
     5221 atgtatcaaa agattgcgga ttatacattt tgctgtaatc tgcgttttct tctgcacttt
     5281 ttacatgagg agcaatcaat ttatcaaatt gcactgcttg ttccgtgatt gcttctgcct
     5341 gacttttttc tttgccggca agctctaata actgaacagc catatcaaag aaaacgccta
     5401 ttagttgtgc accgttggga tggtctgatg cataatacgt cttatctgga aggaataaag
     5461 aaggcggata agcaaagaag gcgttcgttt gcgcattctt catatctgca tctacatcca
     5521 gattaaatgg caatggtaat gtatccaaca gccattctgg aaattgctta ttcaattcgt
     5581 cgaaagactg caatgattca actttttcga ttataggttg aagcggttga gcatgcaact
     5641 catttcttcg gtcataatcg tttgccagtt gataatatgc aaggaaatgt ttcatttttt
     5701 ctgaagacac tttttctgga tgggcaagca gatcgtctgt ttctcccatc aaaagttggt
     5761 cgatcccgtc aactaaatct tggaaaccgc ctgtagctgg tttatctgca gggatttttg
     5821 ctgttttttg ccattcttca ttgactgctt catagaaatc ttgtttcaat cgttctttgt
     5881 tcattcatcg atcgctcctt ttttgtttcg tgtgcccatt atcttacaaa atgaaggaat
     5941 taacaaatag aacataaaaa attgaaaatt cgaacaatca aactgatttg tttttttcaa
     6001 tccttcactt ataatgaaaa aaaggaggcg atcccaactg aatcaagtag tggataaacc
     6061 tttatatttc aaagtagata aaaaaattaa aaaattagcc agcactcagc agctgcagtc
     6121 aagaaaaagt aaattgcttt ttttagctct ggtttttgaa gatcaaagtt atgtgattat
     6181 cgatcaatca ggacatccgg tagaatattc tccagcaaaa tatacttatc aagagggtct
     6241 ttcttgtaaa aaatggaagc tgatcaatga agagccaatc gagttatcta gatggatcaa
     6301 cagaaaagaa gatattcccg ttttgatcga agaaaaaagg tcaggaaagg aactcgcaaa
     6361 ttgctgggtg ggattgccag aagaacgttt tttgaggtat aagaaatggg ctaccccctc
     6421 aggctatctt tgtggtactt acgcggcagc agttctcctt gcctattatc aagactatcg
     6481 taaggggtgg atgcttccaa atgagattcg caagaaaaat acggctgatt cgttggtttt
     6541 gacgaaggca ttgcgtagcc agatccaacc actaggactt cctacgattc cttttcaggt
     6601 tagtacaggg atttctagtt ttttaaagaa gaatggcaat catgagcgtg cacgagcaac
     6661 tcttctaggt tcatggcagc gagctacaaa acgtatacgt gaaggcaaac ctgtgatgat
     6721 tggtattttg aaagtacttg gcagtacata tggcaatcat tgggtgacag cgtacgctta
     6781 ttttgaaact gaaacaggag aacgttatta caaagtacac gacaactggg gcgattatca
     6841 taaagtcatt cctgccagtt ggagtaacgg aacagtttca cttccttaag aatttgctga
     6901 aattaacaga aaatcttgga aaaatatgtt aaactatcga agaatagaga agttttgggg
     6961 aggactacat gagtaataag tatcaggatg acacgttgaa gatttttagt ttgaatggca
     7021 accgtccatt agctgaaaaa atcgcgaaag tgttcggaac agaattaggt aaaagcgtcg
     7081 tgaagcaatt tagtgatggc gagatttcca tcaatatcga agaaagtatt cgaggggatc
     7141 atgtgtatat cgttcaatca acgaatgcac ctgtcaatga ttactatatg gaactactga
     7201 tcatgatcga tgcaatgaag cgagcaagtg cgaaaacgat caatgtcgta ttgccttact
     7261 atgggtatgc taggcaagac cgcacggcga aacctcatga accgatcact gcgaaattga
     7321 tcgctaactt gatcgaagaa gcaggggcaa ctcgagtact gactttagat ctgcacacgg
     7381 ttcaagtaca aggattcttt gatattcctg tagataattt atttacgatg ccgttgtttg
     7441 ctcattatta ccgtcaatta ggtctgacgg gagatgacat cgtagttgtt tcgccaaaaa
     7501 acagcggtgt tcagcgtgca cgtagtcttt cagaatattt gaacagcaca ttagccatcg
     7561 tagatcatgc agatgatgct tcaactgagg gtggttatgt gatcggtgat gttcaaggaa
     7621 agacatgtat catggtagat gatatcttga acacaggagc aacgcttgca cgagcagcaa
     7681 atgtattaaa agaaaacggc gcaaaagaag tatatgcctg tgcgtcacat ggattattgt
     7741 ccgatcatgc gaaaagtatt ttagacaatg caccaatcaa agatatttgt atcactgatt
     7801 ctgttttcac agaagaaaat cgtcatccag aaaacttaac aatcgttaca tgttcagaat
     7861 taatgggtga agcggtcaaa cgcatccatg aaaatacacc gatgagtccg ttattccgtc
     7921 tggaagaaaa agaattcgaa taaacaaaaa aaagagggaa agaagttgtg acagaagtga
     7981 gcagcttcaa ggagctgcga caagactttt gtcacagctc cttttttcta tgcccatgaa
     8041 acgtcctcta gacaaaaatg agctcttctt tggtttgttc gctattttat gctagaatgt
     8101 gcctagcaat aatttggaag ggatgaatga agtgaaaact atttatctgg atcatgcggc
     8161 aactaccccc atgcatccac aagtaattga agcgatgacg gcaatcatgc aagacacgtt
     8221 tggaaatcct tcaagcatac atggatttgg tcgccacgcg catgaaaaat tggaaacagc
     8281 aagacaaatc attgcagaca gtctgcaagc aaaacctcac gagatcatct ttaacagtgg
     8341 gggaacagaa ggggataata cagctatctt agaaacagcg ttctcgcgtc aaaaagaagg
     8401 cagacatcta atcactacag ctatcgaaca tcctgctgtt cttaatacca tgaaatattt
     8461 agagacaaag gggtttgacg ttacatatct gcctgtagat gaaaaaggga atctgtcgat
     8521 cgaagaggtc aaaaaagcgt tacgagaaga tacgatcttg gtctctgtga tgtacgggaa
     8581 caatgaaatc ggaaatttga tgccaatcaa agaaatcggt gaacttttag cggatcatcc
     8641 agcttatttc catacagatg cggtacaagc gtacggaaac caaacgatcc atccagaaga
     8701 attaggaatc gatttattaa gtatctctgc tcataaaatc aatgggccaa aaggcgtcgg
     8761 tttcttatat aaacgagacg gagtaaatat ccctgcattg cttttaggcg gggagcagga
     8821 agaaaagcgg cgtgcaggta cagaaaattt agcagggatc tggggtatgg caaaagcagt
     8881 tgaactgctg tcacctgaag aacgtgaagc acgtaacaac aaatataaag gattccagca
     8941 attgattctt gatacattag aagcaaacga aatcccttat caagtaaatg gtgatttaac
     9001 gaataaactc cctcatgtgt tgaatcttcg cttaattggc gaagcaaatg atttgctact
     9061 gatgaagctt gatcttaatg gggtcgctat ttctactggt tcagcttgta cagcaggaaa
     9121 tatcgagcct tcacacgtat tagaagcaat gtatggagaa gatacaccga tactaaaaga
     9181 atccatccgt atcagctttg gtttgggaaa taccgaagac gacatccagt atttccttga
     9241 tcaattagtt cgtgctgtca aaaaataact agtgaagtca ttcttatatg aacagatgtc
     9301 tgagagaacg ccttcatttt ttagggaact taactgaaaa acctagtaaa aatcaggata
     9361 aacttttata ataaagagaa actaatttta tcgaggtgaa aatcatggca tttgaaacaa
     9421 cagcaacggt actgggcgca ccaattagct atcgtttgca tcctgatgca aaaagatata
     9481 cattgcgcga taatggattt acagaaacaa acggaggcaa ttatcaattg atccgaccat
     9541 tagatgccac gccgcaaagc aaagaaggat tcaaattaaa aatcactgta tcaaaagata
     9601 ttcaaacatt gaaaatgtcg atcactacag caaatggatt gaaagcagtc aatattttca
     9661 aagatccaaa acagaaaatg agccaagata aattttattt tctgatggat gggatgatta
     9721 gccgtgggtt attcgaaaaa gtcgaatgat ctgtggatat aaaaagacaa ctagttttga
     9781 aacggcctat tgttagatga ctaactttgt aaggacgacg caaattagtt gtctttttta
     9841 tttttaaatt taacacatgt tgttattttt aatattttgc tgatttattg tttctaagta
     9901 aagaaattta aattgaaacg attgttattt agggaaaaag gtattttttg aaaaaagaat
     9961 cccgtataat gattgttgtg caaacgctat acaaaatgaa aaagaaaaga gggatgttta
    10021 tggaaaatga acagtcggtc gttttaacga attggaagag aaattatttg ttctttttat
    10081 cgggacagtt tttgtcagga atcactagta tggtcgtcca atatgcgatc atctggtatt
    10141 tgacaagaga aaccggttcg gcgacaattt tgagttttgc tactttacta ggaatgattc
    10201 ccatggtttt actaagccca tttgtaggac cgctagtgga tcgctgggat aaaaaagctc
    10261 ttttgattgt cacggatatt attgtagcga ttttcgcgct tattttagca gtagtcggaa
    10321 cgatttcaga atccttcccg atttggctag tttttgtgtc tttgtttatg cgttcggtcg
    10381 cacaaacatt ccaaatgcca acgatccagt cgattatgcc gacaatcgtt ccatcttctc
    10441 acataacaag gacgaatgga cagttaggaa tggtccaatc agcgaatttc atcattgcgc
    10501 cagctctagg tgcggctttg ttttcagtcg ttcctgtcaa ttaccttatt ttattagatg
    10561 tattgggggc agtttttggt gtcggcttat tgatttttgt gaagatccca aaagtttctc
    10621 ctgaaatatt ggaagttccg cttactattt ttaaagatgc aaaatttggg ctgcagcaat
    10681 tgatggataa taaagggtta tggtacataa cgatcaatgg tgcatttgtg atgctgttgt
    10741 tcatgccagc aatcagtctg tatccattga tgacgctaga ttactttggt ggttccgttg
    10801 gacaggcggg agcagtggaa gtagtctacg cagtcggaat gctcttaggc ggtgcactga
    10861 tcagttttat aggaacatgg aaagaccgaa tgaaacctat tattatagcc tacatcatta
    10921 tgggcctgac gatcggtgca agcggattgg ttccaaacga tagtcaaggg ttcttgtact
    10981 ttcttatctt gaatgcagga gcaggttgtg caacgccata tttcaataca ctgctgatgg
    11041 caatgatcca gcagagttat gaatcgaatg ttttaggtcg tgtcttaggg aacttcaact
    11101 ctttgatgaa cctggcaggt ccaattggat tattatttgc aggaccatta gctgatcgtt
    11161 taggggtaga aaaaatgttc ttgttttccg gaatcgggat tttattatgc ggaatcgttt
    11221 tatttttaac gtcagctgcc cgaaattatg ataaagaatt acagaaaaaa ctggtaaaag
    11281 aacaccatga gcaaaaagac gaatagtatg gaatggagtt gtgacagaag tcttgttaca
    11341 actccatttc tgatcctaat gttcctgtgg caataagcct actcaaatga aaaatatgag
    11401 taattatctt tatttgttta atacttgata tagggcagtt ttttcacgac ctatcgtact
    11461 tcctctttgc ttcatagatt tctttgattt gttgctcaga taacggagtg ccaaatggta
    11521 gtttttcctt taagagttct ctttccggtg cgtctacgcc tacattgaca tcgttctcga
    11581 taggtacgct gtaatgatgg atatgtccat gaagattgcg gatagtgtcg ttaggcccga
    11641 gcaacatcgg ataatgagtc aagtaatatt gttgatggtc gaatttaacg atggcaccta
    11701 catcataaaa ataaaatttg ggacttccgt cagtaagcct aggatcattt ttttctaaat
    11761 aacggaaaaa agcgcgactg tcatggttcc ctttgatcaa gtggattgtt ccttgcagtt
    11821 gagtgaccag ttccaaaatt tctgcatttg ctttttcata ttgcggatgc atagcaaggt
    11881 cgcctagatg ataaacatgg tcggtttctt ttattactgc attccagcta gtgatcaact
    11941 gttggttcat ttcatccaca tttgcaaaga gccgcggagc aaagtcgttt tttcctaata
    12001 agcgttcatg gaaaaagtgc atatcactga taaagtaatt catgtatctt ttgcctccct
    12061 tttttcttag agtaaacaaa atcacttatc tgagcaactc ataacatcta aaaagaaaag
    12121 gttgtaaatg agacgtgcag ctttaagcga taagaagcaa ttttgaaaaa tagtttttca
    12181 caatttataa aattattcct tattgccgaa aggctggccc tttttatgag tatgccaatt
    12241 ttttatatcg aatgctcaaa agatgagtga ttttttggtt gactatcatg tttgtgtact
    12301 gaataggtcc agtagaacag tatttttacg acttgataat taagggatga aattttcaga
    12361 aatatgacgt ataatgtaaa atactgaaaa gttgcgacct tcaactcgta gatgtttcag
    12421 aatctaattg aaatgatggt gatatcatga cagacaacag caaaacacgc gttgtcgtag
    12481 ggatgagcgg cggtgtcgac tcatctgtaa cagcactttt gttaaaggaa caaggatacg
    12541 acgtgatcgg aatattcatg aaaaattggg atgacacgga cgaaaacggt gtatgcacgg
    12601 ccacggaaga ttataaggat gtagcgaaag tagctgctca aatcgggatt ccttattact
    12661 ctgtgaattt tgaaaaagaa tattgggatc gtgtatttga atatttccta gccgaatacc
    12721 gcgcaggacg tacacctaac ccagacgtga tgtgcaacaa agaaatcaaa ttcaaagcat
    12781 tcttggatta tgcaatggat cttggcgccg attatgtagc tactggacat tatgcgcaag
    12841 tcactcgcga tgaaaacggc gtggtccaca tgcttcgtgg agtagacaat aataaagatc
    12901 aaacttactt ccttagccaa ttatcacaag aacagctttc aaaaacgatg ttccctctag
    12961 gcggcatgga aaaatcagaa gtacgggcaa ttgctgaacg cgcaggcctt gcgactgcta
    13021 agaaaaaaga ttcaacggga atctgcttta ttggtgaaaa gaacttcaaa caatttttga
    13081 gcaactatct gccagcaaaa aaaggaaata tggtcactct tgatggtgaa gtcaaaggac
    13141 aacatgctgg cttgatgtat tatacgattg gtcaacgtca aggactaggg atcggtggcg
    13201 gaggagattc gcaagaaccc tggtttgttg taggaaaaga tctagcgaca aacacattat
    13261 atgtggggca agggttccat catcctgcat tgtatgccac aagcttggat gcaagtgaaa
    13321 tccactttac gacgaatgaa ccaatgccaa aagaattcaa gtgtacagcg aaattccgtt
    13381 atcgtcagca agacgttcct gtgacagttc gcttattgga tgataatcgt gcagaagtcg
    13441 tattcgacga acctgtacgt gcaatcactc ctggacaagc agttgtattt tatgatggaa
    13501 tggaatgtct gggtggtggt ttgatcgacc acgcatatca agaaacaaaa gttttacaat
    13561 acgtttaagc gatattaaat gaatcaaaac agctatggct aacaaaagaa acaccttttc
    13621 ttttggatag catagctgtt tttttgcaat aaaattaatt tttattcatt ttatacttga
    13681 aatacttgtg taaaaagttc ataatatcca ataatatgaa taaagttgct ttttgttaag
    13741 caagagcagt tgcaaaatag aagggaatct tttgcaagtg atttgttcaa ttttataagt
    13801 gagggtgatg gaaatggaaa agaaatcgac gggtattagc aaatggggac tggttgctct
    13861 ggttgtcagt tcgtcaatcg gaagcggtgt gtttgggatc acaagtgatc tggctagttc
    13921 tgcagctcca gggccagcga tcctttcatg gataatcgtt gggattggta ttttagcttt
    13981 ggtattatcg ctgaatcatt taggagaaaa gcgtccagat ctagatggtg gtattttcgg
    14041 ttacgcggaa gcttcatttg gtaaattagg tggcttcatc agtggttggg gttactggtt
    14101 gtctgcgtgg ctgggaaatg ttgcttttgc aacgatgctg atgagcgcca tgggagaatt
    14161 tttcccgata ttcaaaggtg gacaaaatgt tccttctatt ttatttgcaa gtatttttat
    14221 ttggggtctg actttattag taaacaatgg agttgaagga gcgagcgtca tcaatacaat
    14281 cgtgacgatc tgtaaattag tgccattatt cctattttta atatttgtat ttatcgcatt
    14341 taaagtgaat atattcactg ctgatttttg gggtaacgta tctgataatc tagtgaatgg
    14401 aacagggcag caaggaacga tctggttgca gattaaaggt tgtttgatgg ttatgatgtg
    14461 ggtctttgta gggatcgaag gcgcaagtgt tttagccaac cgcgcgaaga aacgttcaga
    14521 agcccagcaa gcatcgatca ttggtctgct ttgtttattg tttatctata tccttgcttc
    14581 gatcttgcca tacggcgtct tatctcaaga agagttagca actatttcac aacctgctat
    14641 ggcaaatatt ttaaaaggaa tcgtgggaaa ttggggagca gcgctgatca atatcggctt
    14701 gattatttcg atcattggtt cttggttgtc ttggacgatg ctcccagcag aaacaacgat
    14761 gttgatggct cgagacggtc tgttgccgaa aggttgggga aaaacgaata aaaaaaatgc
    14821 cccaacttat tcgttagttg ttactgcaat cttgactaat ctattcttat tgactttctt
    14881 agttactgat tatgcctatc aatttgccta ttctttatgt acagcggcaa ttttgatttg
    14941 ttatctactt gtcgggatct atcaagtcca gttttcttta aaagaaggaa tcaaaaaaca
    15001 agtagtgatc ggtgggattg ctgtagtttt tgaattgatg ggcatcattt tagcaggatt
    15061 ttcttatgtc cttttatgta gtattgccta tcttcctggt ttttgcttct actggatcgc
    15121 ttgtcgtgaa accaaacatc aaattacggg aaaagaaaaa gtaatgattg ggctgatttg
    15181 tgtggccgca ctgctttcaa tcgggttgct tgctgtaggc tggatcaaga tataaggggg
    15241 aaagaaacat gaaaatcgca aatttcggtg tagaagaatg gctaaataaa tgggaaaaga
    15301 aagcggtata tgatattgca caaagctcga tcgaagcatt gacattagac gaactcgttg
    15361 gcttagatgg gactagcgta agtaaatttt tcgaaaaacg caaaaacgaa ccattggact
    15421 atggctggat cgaaggatca gatgttttca aacaagaagt cgcttctctt tatcaaaccg
    15481 tcgatccgga aaacatctta caaacgaatg gagcgactgg tgctaatctt ttagctctat
    15541 acgcgttagt ggaagcagga gaccatgttg tttcgatgct tcctacttat cagcaattat
    15601 atgacatccc caaatcttta ggtgccgctg ttgactttgt tcatttgaag gaagaagaag
    15661 attggcagtt tgatttagaa cagctagaag ccttagtcaa acctgaaaca aaaatcatct
    15721 gtttgaatag tgcaaacaat cctacaggca cattgctaga tcggaaaact ttagaaaaaa
    15781 tcgcagaaat tgctcgtaca gtagatgcat atgtattgat agatgaagta tatgcgcctt
    15841 taacggataa aggtgaattc ttgtcgatcg tagatgtcta tgaaaaaggt atagcaacca
    15901 attccctatc caaaacctac tccattccag gtattcgtat tggctggaca gctactggac
    15961 ctgagttggc agaagttttt cgaaagtatc gagactacac gatgatttgc ggcggagtcc
    16021 tgtcagatga tcttgctgta catgcattga agaataaaga aaaaatattg gcgcgaaaca
    16081 aaaagatcat tacagagaac cttgtgattt taaaacaatg ggtagccaat gaaccgaaag
    16141 ttgaacttgt cgctcccaat tacgtctcta cttctttcat taaattagcc atcgaagaag
    16201 atgaccagac attttgtatc aatttattag aagaaacagg agtactatta gtaccaggaa
    16261 gtgcctttga tttaccgaag catgcccgac taggttactg ttgtaaaaaa gaaaccttgg
    16321 aaaagggatt attcctctta tctgagttct tgaaaaaaca ggcctaaaca aaaacactgc
    16381 aaggacagtc tgtgacaact gttcaagcag tgtttttcat ttacgcttat tgtctatgag
    16441 aaggagagtg ccctgaagct atggagactc ctgaagcttt ttcttctgca gtttcttgct
    16501 gatggatctc ttgtgtattg tgtcgtacaa gcaagaagtt gaccaaaaca agaagagaag
    16561 tcgaaagaaa gacgccgcga taaccaaatg cactggaaac actggagccg atcataggcc
    16621 ctactacgtt accagttgct tggaaagatt ggttataact gaagatccgt ccagcagctt
    16681 gatgaggcga atattttgtg atcaatgctt ggacagcagg gagcagacaa gcatcagaga
    16741 taccaatcaa aaaacgtaag gcagctaatt gccaaacatt cgtgacgaat gccattggga
    16801 tataaaccag aatcgcaaaa cccaaaccaa tcgcaagtat tctttcactc ccaatcttgt
    16861 cacctaagcg accaaatctt ggagcggcta tcaaagtggc aatcccaggt atcgaagcaa
    16921 tgattccgct gatcaacgta acattcccat gtccatgcag caattggcga atgtacagac
    16981 tgatgattgg actaatggaa ttgttggatg cttggatgat catcgttgtg acgaacatac
    17041 cgatgacgac gtgtggatat ttcaagtctt tgaaaatctg tttggcagaa accatgtttt
    17101 cttttttcac tgggacaaat ttttcatgga cgaaaaacag actcaacaag aagacgaaaa
    17161 ataagatgat ccctgtaatg aaaaaggtag ggcgatagcc aaacgctgaa gcagagatcc
    17221 cgccaagaag cggtccaagt aatgttcctg taacagaacc agttgctaag gttccaagaa
    17281 cttgaccact tttttctttt ggcgtcccag ttgcgacaag ggcagtggca ttactgatat
    17341 aaccagaaaa aatcccttga agcagacgca atgcgactaa ttgatacaca ctagtcacgc
    17401 aacccatcaa agaaatgacg attgccattc ctaatgaagc acgcaacagc atcaattttc
    17461 ttcctttttg gtcggctaat tttccccacc aaggagagat aagcgtagtc accaagaaag
    17521 tagatgaaaa agtcaatccg ctccaaaagt tcaattgtga cgtactataa ttacctaacg
    17581 tatctatgta aagtgacata aaaggcatga ctaaactaaa tccgatacca gccataaatg
    17641 ttccaaacca taagacaaac aagttttgtt cccaagtttc tcttttccta aataattttt
    17701 cctttattct tgcagccaat tagactcctt ctttctgaaa attttcatct acagctcttt
    17761 attataaact acaatatgta gtttatcaat gaaagtctct tttttctctt tctttttcgt
    17821 ggtaaactga aagagtgaag gatttatttt atgagaggaa acatggaaat gaaaaaaaga
    17881 aatctgtctc aagagaaaat catcgactgc tttcgagaac tagcagaaga gatggatgtt
    17941 cagcaaataa cctttcagca tttagccaaa cgcttggata tcaaggctcc ctcgctgtat
    18001 aaccatttca aaaatattcg tgatgtcaaa acagcgctaa cagcgaaact tctgcaggaa
    18061 cttaatgacc agctgcgacg ggctcttgtg ggaaaaagtg gtcaggaagc actgcagatc
    18121 tatgcagaaa cttatcgatc ctttgctttt tcaaatcaag cagtgtatga gttactgatc
    18181 agtgtaccgc ataccaatga agctgtttta ttagatggaa tccatgaaac gaatcagatc
    18241 attttgcaga tatttgaggc ttttcaatta acgaaagaag aaaaagttca tcgaagcagg
    18301 gaacttcgta gtatgatcca cggttatctt tccttacgtt ttttaggata ctttacaaaa
    18361 gaaccaacaa tcgatccaga agacagctat ttttggatga tcaataactt tatcgctact
    18421 ttgccttaaa caatcagatt catttgtaac aattttaaga tgttcattaa atgacccttt
    18481 tataagagaa aattcttgca actttcttga aaagtgacta agatgagtct agaaaagctg
    18541 aaagaaggag cacgagtatg tatcaagtaa ttaccatgtt cggggacaat gagccttggt
    18601 ggttttttga agactgggaa gaagatattg aagaagaaga aacattcgag tctttcgaag
    18661 aagcacgaca agcgtacgaa aaacaatgga acgaaatcca tcaaaattat gaatatatca
    18721 atgcaaaaag taactttctc agcgcctttt ggaatgacgg agatgagcgc tggtgtgaag
    18781 agtgtgacga tgatttacag caatacaagg gacttgcgtt gttgaaaaat catcagccca
    18841 tcacagtaga aagcagaaag gaattttatg aaacaactaa ttctagcgga aaaaccaagc
    18901 gttgcgaaag acctaagcaa ggtgcttggt gctaatcaaa agcacaaaaa ttattatgaa
    18961 ggacccaagg tgatcgttac ttgggccctt ggtcatttat taggattgaa aatgccggaa
    19021 gatctgaata aggaatggca gagctggcaa atggacacgt tgccaatgat ccctaaaaaa
    19081 ctaggaatca aaccacttcc taaaacagga catcaattaa aagcaatcaa gcagctggct
    19141 cagcgaaaag atgtctcaga agcagtcatt gcgacagatg ccggaagaga aggggaactt
    19201 gttgctcgct ggatcttaga gtatgtgcat ttcaataaac cagtgaagcg gttatggatc
    19261 tcttctcaaa cagataaagc aatcaaaact ggattcaagc aactaagacc tgcgaaagaa
    19321 tatgatgcat tgtatgaatc tgctcttgcc cgggcaaaag cggactggct ggtaggattg
    19381 aatgtgacca gagccttaac tgtgaagtac gcggataatt tatcagcagg ccgtgtgcag
    19441 acaccgacac tagcattagt gagagaccaa gaaaagaaaa tcgagcagtt ccgtccacaa
    19501 acgtatttca cgatcacgtt gcaagctgga aaagaacaag ccaaaatgct tcaaaaaaat
    19561 caatttgctt taaaaacaca agaagaagca caaaaacttg ttcaattgat cagtacaagc
    19621 aaaggaactg tcacagaagt ccaagaaaaa ttaaaaacgg aatctgcgcc attgccttat
    19681 gatttaacag agatccaaag agaagccaat caacgctatg gctattctgc aaagaaaaca
    19741 ttaagtcttg tacaaagttt gtatgagacc cataagatcg ttacttatcc gcgtacagac
    19801 agcaaatatc tgacaaatga catgaaaagt acgatgaaag aacgattgca agcagtcagc
    19861 gactttgctc ctgaggtcaa gcagtatttg aaaaacggag ccatcgtcca acagacaaaa
    19921 gtattccaag acagtaaagt gaccgatcac catgcacttt tgccaactga aaacagagca
    19981 cgttatgaaa aattaagtaa tgaagagcga aaaatctatc aaatgattgt ctctcgtttt
    20041 ttaggactgt ttgctcaacc ccataaagtc agccaaacaa aagttacggt tgaatttggt
    20101 aaagaacgat tcatttttcg gcaaaatcgt gtcgtccaag caggatggaa aggcgaaact
    20161 gaaactgaga cggaaacagt caaatgggaa aaaggaatga gaatcaatcc cgatttcgcc
    20221 atcaaaaaag aactatctgc accgcctaaa ccattgtcag aagcaagctt gcttggtcaa
    20281 atggaaaaat acagtctggg aaccccagct acgcgtgcag agattatcga gaaactaatc
    20341 aaatctgaac tgatggaacg tacccctcaa ggactgcaag tatctcctaa aggtaagcaa
    20401 ttattagtat tggtaaatcc ctctttagta acaccagatt tgacagaaag ctgggaaaaa
    20461 gaccttgaag ctattgctgc aggaaagaaa caagcaggaa ccttcttgaa tggaatcgaa
    20521 aaagaaacaa aacgcttggt gaatgagatc aaatcaagca aacaagaata tcaagatttc
    20581 tcgattaccc aaaagaaatg cccagaatgt ggggccaatc tgcgcgagaa aaacacacgt
    20641 gacggcaaaa tctatgtctg caccaattca gattgcaact accgcagaag aaaagatcca
    20701 aaagtctcta atcatcgatg cccgcaatgt caccggaaaa tggaaatcat cgaaggtaaa
    20761 aacggtgctt atttccgttg taaatacgat ggaacgacag aaaaattgct tgataaaaaa
    20821 gagcagaaga agaaaatgac caaacatgaa gaacgacgct tgctgaaaaa atatgcacaa
    20881 gcagaagaac cagaagaaag cccattagca gcagcactaa aagcagcaat gaagaagtaa
    20941 taagatatga aagaggttgt gatagaagtg gtcagcttca agaaataaga aggaatttac
    21001 gaaaattgct tctcaaattt ttgtgaaatt tcggcttatt tccgaagaag ctacttctgg
    21061 aacaccattt attcggaaaa aaggactgtg acaagacttt tgtcacagtc ctttttcatt
    21121 taaaaaaatg tttacttcat ttgacgtata ttattggtga ggcttcaatt catttggctt
    21181 cactttttct gtttctctta tgatataaac aggacgctgt ttcgtttcta agaagatttt
    21241 cccaatatat ttcccgatga ttccaagaca taacaattgg attccaccaa tgaacaggaa
    21301 gatcgagacc atggaaggcc atcctgatgt aggatcacca aacaacaatg cccgcaaaat
    21361 aatgaacaaa attgcaatac cagaaccgac acaagacaaa gcgccgataa atgaagcaat
    21421 cgttaacggt gcatccgaga agttcacgat accatcaatc gagtagttga ataatttcca
    21481 gaaggaccaa gatgtttcgc cagccacacg ttcgcggttt tcataagaaa tatattctgt
    21541 tttaaatccg acccagctaa ataatccttt tgaaaatcgg ttgtattctg ttaattctag
    21601 gacggcatcc accatttgac gagtcatcaa gcggaaatca cgagcgccat cgaccatttc
    21661 tgtatcgctg attttattga tcaactggta gaattttttc gcaaagaaac tgcgtatcgc
    21721 aggctcgcct tcacgagtcg aacgccgggt gccgacacaa tcgatatcag aagtagtgat
    21781 ccggttgatc atctcaggaa gcagttcggg cggatcttgc agatcaacat ccatcacggt
    21841 cactaaatct cctgtagaag cttgcaatcc agcgtacaga gccgcttctt tcccaaaatt
    21901 tctggaaaat gagacaaagc ggacataagg ataagctgag gctagcttct tgattgctgc
    21961 taacgtaccg tcttttgatc cgtcgtcaat aaacaaatat tcgaactcat gggcaccttt
    22021 gaatttttca acctcatcaa aaaaaagagg aacagttttt tcttcattaa aacatgggac
    22081 aacaattgat actttcatcg tttctatcct ttcaatcgta tgtttatata tggtttacat
    22141 tcaaaattta ataaacctta aataacacct ttaaaatttg attttacgta aaagaacagg
    22201 tattcttttt gtaaactaac gtatttagtt taccatgttt cctcggttta tcaaactttt
    22261 aagaacaatt tttattagtt ggcaagtagt tttggataga gaaaaaggag gaagttccag
    22321 tctattgact aggagcaaac aaaatgaaaa tacacacacc tatcggcttt ttatggttag
    22381 atgcagatga atccggattg actgctgtat cttttcagcc aatttcagaa agcgaatcag
    22441 cgaatcagcg aattttaacg cgcacacaag aacagtttga agaatatttt gcgggaaaaa
    22501 gaaaagaatt tgatgttcct ttatctataa agagaggtac gccttttcag caaaatgtct
    22561 ggaaagcttt gtcgaccatt ccttatggag aaatccgaac gtataaagaa gtagccgttg
    22621 ctgttgatag tccgaaagct gtgcgagcga ttgggcaggc aaatcgaacc aatccattgc
    22681 caatcgttat tccttgtcat cgagtgatcg ggcaaaatgg aaaactgaca ggatatatgg
    22741 gaaacgcaat agatggggtt gaaatcaaac gccaattgtt gttgttagaa ggatatctgc
    22801 aaaattaaaa acagttgtga ttcggacatt gttccatgcg agaatcacaa ctgttttttc
    22861 atgttcattc taaactttga tcagccaaat aaatagtaaa gccatcactg tttggagaag
    22921 cccgacagct aatccataag ataagaagcg tgtaccttct ttcatgaatt ttgcaaagtc
    22981 taaacgcaga ccaattgccg ctaaagcagt gatctcaaac cagctgctga taaaatgtgc
    23041 cgattggtca ataactacag gtaaagaaaa caagctgttg accaaacaaa agatcacaaa
    23101 ccctaataca taccaaggaa gcggtaattt tcgttttttc tgcgtatcag ttggcgtttc
    23161 aaacagagct ggttgtttct tgtgcatgaa tttttcaaac agatagacaa caacaacgag
    23221 catcataata cgtagaatct taaatagcat ggcgaattcg acaacagaat tgtcaagtaa
    23281 gcttgcccca gcgactactt gaccaacaga ttggagtgta ccacccaaaa gtgcactttt
    23341 ttcaatgaga tttccaccaa acaactgtaa tccaagaaaa gggagagtca acatcatcac
    23401 agtacccagt agattgacta gtgtgatgat ctgtcctttt tcttcctctt tcgcatggat
    23461 ggaaggggcg attgaagcaa tagcagaaga accacaaaca gcgtttcctc cagccatcat
    23521 caaagaagaa gtatcgctga attgcagttt tttaccaatc aagtaagcac cgatgatagt
    23581 gactgacatt tgcagtaaca caaaaacaat tccttttaca ccaatttgag caatcgtttg
    23641 agctgtgaca gttgcaccta gtaaaaaaac agagaattct aatagtttgc tttcagcaaa
    23701 ttttgtgccg attgctagaa caggctgttt aaaaaaagta tttcctaata aaatacccaa
    23761 taaaatagca atagtcgctc cacctaactg tggcagataa atagcaagaa gcctgctgaa
    23821 ggcagcgatg gcaaaagaaa gcagtaaacc gggtaagatt tcgcggaccg ctgaatcctt
    23881 tacagatttt attggatgtg acaatgattt catttgatcg gttcctttcg tcaataaata
    23941 ctaaaagcat actcgaacat agtagcacta aaccagtatt ttgacaaaag aacaagaggt
    24001 cgtgacaaaa gtcttgtacg acctcttgtt tcgaata
//
LOCUS       DAMEET010000041        23828 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.041, whole genome shotgun sequence.
ACCESSION   DAMEET010000041 DAMEET010000000
VERSION     DAMEET010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.041"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(53..787)
                     /locus_tag="PB780_001437"
     CDS             complement(53..787)
                     /locus_tag="PB780_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDE0733498.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="PB780_001438"
     CDS             1114..1524
                     /locus_tag="PB780_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDE0733499.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="PB780_001439"
     CDS             1517..2218
                     /locus_tag="PB780_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDE0733500.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="PB780_001440"
     CDS             2218..3804
                     /locus_tag="PB780_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDE0733501.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="PB780_001441"
     CDS             3823..4044
                     /locus_tag="PB780_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HDE0733502.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="PB780_001442"
     CDS             4041..4802
                     /locus_tag="PB780_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HDE0733503.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="PB780_001443"
     CDS             5182..5691
                     /locus_tag="PB780_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDE0733504.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="PB780_001444"
     CDS             complement(5766..6305)
                     /locus_tag="PB780_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDE0733505.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="PB780_001445"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="PB780_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDE0733506.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7318..8169
                     /locus_tag="PB780_001446"
     CDS             7318..8169
                     /locus_tag="PB780_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDE0733507.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8197..8832
                     /locus_tag="PB780_001447"
     CDS             8197..8832
                     /locus_tag="PB780_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDE0733508.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="PB780_001448"
     CDS             8852..9625
                     /locus_tag="PB780_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDE0733509.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="PB780_001449"
     CDS             10033..10830
                     /locus_tag="PB780_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HDE0733510.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="PB780_001450"
     CDS             10808..11221
                     /locus_tag="PB780_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDE0733511.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="PB780_001451"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="PB780_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDE0733512.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="PB780_001452"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="PB780_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDE0733513.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /gene="maf"
                     /locus_tag="PB780_001453"
     CDS             15998..16558
                     /gene="maf"
                     /locus_tag="PB780_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HDE0733514.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="PB780_001454"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="PB780_001454"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HDE0733515.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="PB780_001455"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="PB780_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HDE0733516.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="PB780_001456"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="PB780_001456"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDE0733517.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="PB780_001457"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="PB780_001457"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDE0733518.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="PB780_001458"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="PB780_001458"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDE0733519.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="PB780_001459"
     CDS             21905..22483
                     /locus_tag="PB780_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDE0733520.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="PB780_001460"
     CDS             22535..23017
                     /locus_tag="PB780_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HDE0733521.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="PB780_001461"
     CDS             23134..23508
                     /locus_tag="PB780_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733522.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7781 a         4285 c         5115 g         6647 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaga
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcacccggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaataa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagttt
//
LOCUS       DAMEET010000042        23667 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.042, whole genome shotgun sequence.
ACCESSION   DAMEET010000042 DAMEET010000000
VERSION     DAMEET010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.042"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            93..1520
                     /locus_tag="PB780_001462"
     CDS             93..1520
                     /locus_tag="PB780_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDE0733523.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQVSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRLTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERTDRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1612..1977)
                     /locus_tag="PB780_001463"
     CDS             complement(1612..1977)
                     /locus_tag="PB780_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HDE0733524.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQNDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1974..2339)
                     /locus_tag="PB780_001464"
     CDS             complement(1974..2339)
                     /locus_tag="PB780_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HDE0733525.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            2777..2905
                     /locus_tag="PB780_001465"
     CDS             2777..2905
                     /locus_tag="PB780_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HDE0733526.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2962..4656
                     /locus_tag="PB780_001466"
     CDS             2962..4656
                     /locus_tag="PB780_001466"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HDE0733527.1"
                     /translation="MRKSKSIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKMTNTLTQ
                     EEEHVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKSNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4660..4779
                     /locus_tag="PB780_001467"
                     /pseudo
     CDS             4660..4779
                     /locus_tag="PB780_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            4783..4902
                     /locus_tag="PB780_001468"
                     /pseudo
     CDS             4783..4902
                     /locus_tag="PB780_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            5430..6563
                     /locus_tag="PB780_001469"
     CDS             5430..6563
                     /locus_tag="PB780_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDE0733528.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNVLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYE
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6594..6884
                     /locus_tag="PB780_001470"
     CDS             6594..6884
                     /locus_tag="PB780_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HDE0733529.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKQ"
     gene            complement(6944..7403)
                     /locus_tag="PB780_001471"
                     /pseudo
     CDS             complement(6944..7403)
                     /locus_tag="PB780_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(7533..8786)
                     /locus_tag="PB780_001472"
     CDS             complement(7533..8786)
                     /locus_tag="PB780_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDE0733530.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8815..9132)
                     /locus_tag="PB780_001473"
     CDS             complement(8815..9132)
                     /locus_tag="PB780_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDE0733531.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9158..9478)
                     /locus_tag="PB780_001474"
     CDS             complement(9158..9478)
                     /locus_tag="PB780_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDE0733532.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDIINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9943..11358)
                     /locus_tag="PB780_001475"
     CDS             complement(9943..11358)
                     /locus_tag="PB780_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDE0733533.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREHEERYMDEKGIVQDDYRIEF
                     IGQHLRYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11374..12690)
                     /locus_tag="PB780_001476"
     CDS             complement(11374..12690)
                     /locus_tag="PB780_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDE0733534.1"
                     /translation="MRGSGMSFMDKLAKVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISYLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12840..13538)
                     /locus_tag="PB780_001477"
     CDS             complement(12840..13538)
                     /locus_tag="PB780_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDE0733535.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNIA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNVLAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13827..14787)
                     /locus_tag="PB780_001478"
                     /pseudo
     CDS             complement(13827..14787)
                     /locus_tag="PB780_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            complement(14901..15734)
                     /gene="msrA"
                     /locus_tag="PB780_001479"
     CDS             complement(14901..15734)
                     /gene="msrA"
                     /locus_tag="PB780_001479"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDE0733536.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDTRENISVKEQ
                     LSKLEVTLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQDERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15968..16711)
                     /gene="truA"
                     /locus_tag="PB780_001480"
     CDS             complement(15968..16711)
                     /gene="truA"
                     /locus_tag="PB780_001480"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDE0733537.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYRS"
     gene            complement(16902..17537)
                     /locus_tag="PB780_001481"
     CDS             complement(16902..17537)
                     /locus_tag="PB780_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDE0733538.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(17684..18925)
                     /gene="sstT"
                     /locus_tag="PB780_001482"
     CDS             complement(17684..18925)
                     /gene="sstT"
                     /locus_tag="PB780_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDE0733539.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            complement(19246..19923)
                     /locus_tag="PB780_001483"
     CDS             complement(19246..19923)
                     /locus_tag="PB780_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HDE0733540.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATIATTTTTSTESKPTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSESSEQVEQTLWNADKSAQLEAFMA
                     TWGQTMGQQYKSYTNQMSVDLYGLKVPQVILNGEWKMAIGGVPVSAEWSESGTGQADY
                     QITAVYSDAETEPYLKKNVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELKNG
                     FNQIVYG"
     gene            complement(20174..20716)
                     /locus_tag="PB780_001484"
     CDS             complement(20174..20716)
                     /locus_tag="PB780_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDE0733541.1"
                     /translation="MNIVEEKFAQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLKQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(20721..20948)
                     /locus_tag="PB780_001485"
     CDS             complement(20721..20948)
                     /locus_tag="PB780_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDE0733542.1"
                     /translation="MKKMIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSTKVKEA"
     gene            complement(21027..22907)
                     /locus_tag="PB780_001486"
     CDS             complement(21027..22907)
                     /locus_tag="PB780_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDE0733543.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIIIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTARVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDNVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDQFSQYYTPTVLLIALLVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQRGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAMLKQAGAKHLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLH
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSSFGSLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLIGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            23193..23528
                     /locus_tag="PB780_001487"
     CDS             23193..23528
                     /locus_tag="PB780_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDE0733544.1"
                     /translation="MGIEAVDKYLYLLAGNKIQKSLMDFIQELECTFHKKFTHSILLK
                     LLIHTACLIERTLINGHELKIISEDDTRPSHETIFHVKKAFKNIETEFGITVSYDECF
                     FIYDIIASK"
BASE COUNT         7365 a         4842 c         3987 g         7473 t
ORIGIN      
        1 gctttttcag aaattaactg atgatttcaa aaagaaaaca tgtcataata tttcccatat
       61 tgtgcaaaaa aatacaaggc ggaggctcta ctatgattga agaatatatt gagaaaaata
      121 ttttaagaca gctgttttta tgtgggcaat tctatgtaaa taaagaagtc aatctagaaa
      181 agctctccaa tctcctgcat gtttgcaaaa ccactttgct caatgatatc aataacataa
      241 aaaaagaatt tgaagaacag atcgcttata cacatcggga aaaggaccgt tacactttat
      301 atttttcaga acatatacct cgatgcaaga tcatgcagca agtgtctcaa aattcgttgt
      361 ttttaaaaac ttgtctgctt tatttggaag aagatgaacc tgattatctt caattaactg
      421 aatgtgaatt catctctgtc agcaaagctt attcattaaa aaaacaagta ttagcttatt
      481 ttaatgactg cgggatcgaa atcgaccgct actcccctcg actcactgaa atggaaagac
      541 gtctgctgct tcttaatgtg tcgtatcgtt taggcggttt taattcttgg gagctacctg
      601 aatcgttttt tgaacgtacc gatcgtttca ttgaatccgt aacggaaaat agtggcagat
      661 tttatgataa agaaaataaa gaaatcttat cgattggttt tgctatcagt ttcctacgcc
      721 agcaagtgtg tgctgtgaca atagattcaa aatttatcga agaaatcaaa aaacgtccta
      781 tttacaatta tgtcgaatca gcatgggaaa acactgattt ccaaacctat tataaaaaag
      841 aagaattcgc ctttattttg acactattca atctttgtaa ttatggtttc cattcttatc
      901 agttgattgc ggaagacttt caacagctac accaagtgtt tattgacaat actcccgaaa
      961 taaaagaact agttgctacc tttgaatctc acttcaatca agaattattc ggaaaccaac
     1021 cgttcgaacg tgcgctgatt catctgatgc gttcggcttg ggataactat caattattca
     1081 tgccagaaaa attttatctt ttaaatgagg aacagacaaa tttatataaa gaagtccaga
     1141 caattttttc cagctggtcc agccagttac cttatgatct acggttgaat cctaattgca
     1201 tgcgggcttt tgtgatcgaa ttgagcggaa ttctccgtct gacaaaagaa catctgacta
     1261 tctatatcgt cacaaattcg gatgttcatt atctgattta tcgggaagcc ttggaagctg
     1321 taactacttt cgattttcaa gttgcaccaa caatttattc aagtatatct gatataaaga
     1381 agtatgcgca gcagtcctca aaccgggtat tatgtgaacg tacattatat acaccagatg
     1441 cggtccaata tgaaaatatt attcctattt cgatcaatac gatcgatcga gctatcattt
     1501 cagctgtgca aaacaaataa ggggctgtga caaaaatcat gtcacagcct cttatttgtt
     1561 gaagatgccc atttctgtaa caatctcttg atacacgtca ggctcacaac ctcacttatt
     1621 ttggacatct acgggtccga tctctttatt taaaaaataa gaaatgatgg ttcggataac
     1681 taccaacgcc gctagttgga aaatatcctg caaggtaggt ttgataatcg attcgatgat
     1741 cccagctgaa acaagaattt ccaaacttag aagtatatag cctcccaaca tctttttaat
     1801 agcattgttc tcgctgactc ggtcgttttg cacttttgta tggacaaata aatatttcaa
     1861 aaaatccttg attgatagac aaacacccca aaccaataaa cagatagcaa agatatcaag
     1921 tcccagtaca agtttttcaa aatatggaag aagaaactgc atcaactctg tcgtcatcct
     1981 acctctcctc ttctttgttt ttctttgtct tctatttcct gattcaagaa ataagaaatg
     2041 actgttcgaa tgataataat aagtgcaagc ttcaaaatat ctgtccaagt cggcttgata
     2101 actgactcga tgatatcagc aacgactagt acttccaagc ttaacaaaat ataacttgct
     2161 agcagttttc ttgtatctgc attacgagta ttacgttctg catagttttt cttgacatga
     2221 gaccaatcaa aaagtttgaa tagaacaaga acgatcccga caattaagat accgatagaa
     2281 caaatattta aaatagcaat gatccattct agaaattcaa ttaaatttga gcgaaacata
     2341 tacgctacct actttctttt aatttgttcc tattctttta ttgtaaaaga aagttacctt
     2401 gttcgtctat ttaaaagttg ttctaatgat aacggttcgc ttaaattttt ctttttcact
     2461 ctcccatttt tctcccctca tccataaatc gtggaagtat ccaaaacaac gtagcaagtt
     2521 aaaataaggc ttagtccgtc atcagctttt caaagaagca ctgtctcggt gcttttttgt
     2581 acccaaactt ctaaaaatag aaccatacac ctcctcaaat cttttcttct tacctaccct
     2641 atttttattt acaggaaaat cagacacttc ttcttttttg tcaaaatttc ctttttcttc
     2701 tcgtcctatc atttggcatt ctaataaaaa cgaactatcc ttggatatag aaagagaaaa
     2761 caaggaggcg tttttcatga gtaaaaaatt aatcaaagtc ggcatcggtt taggtttgct
     2821 tgcccttggc gctgcatatc taggaaaaaa gacaggacta ttcgaagatg acagtcattt
     2881 atatgatgaa ttcgaatcaa tctagcatcc tatttcgttg aatcactacg gacatacttg
     2941 aaagacattg gaggaatcac aatgagaaaa agtaaatcaa taatgattgg tgccggtatt
     3001 gccaatatgg cagcggctgt ttatttgatt caagaaggca aatggaaagg ggatcagatc
     3061 accttttatt cattggatga tcacggttca aatgatggtg caccgaccga cactgtagtg
     3121 gatgaatatt ggaataaaaa ccatccatta gaaaaccaaa aaggctatat tgctcgaggc
     3181 ggtcgcatgc ttaattaccg tacctatgta gatttgatgg atctgttaag tcgaatacct
     3241 tctgttactg agccagggat gactgctgaa gaagatacga gagattttga tgcaaaacac
     3301 cgaacctttg acaaagcacg tttgatggaa ggtggcaaag gaatcattga tggcggaaaa
     3361 ctgggattca ataataaaga ccgtattctt ctgactaaac tgattgctat gcctgattca
     3421 gaagaagaaa aactagacaa tatcactatc gaagaatatt tcaaagatga tccacacttt
     3481 tttgaaacaa acttctggtt tatgtgggaa acaacatttg ctttcagaac acgtagttct
     3541 gctcaagaat tgcgtcgcta tatgcatcaa atgatttatg aattcactca aatcgaacat
     3601 ctagtgggcg tcaatcgaac acgttacaat caatacgaaa gtattatgct tcctttgatc
     3661 gagtacttga aagaacaagg atgcaaaatc attttgaacc gccgtgtcac tgactgggaa
     3721 ttcaaagaaa caccgatgca agatgaaatc actgtgactg gtttgaaaat gaccaataca
     3781 ctgactcaag aagaagaaca cgttgtggtc gatgaagata cagcagttat tttcactaat
     3841 gggtccatta cagattcagc tactttagga gattacgaaa caccagctgt agaaaatatg
     3901 gattatggtg cagcttctag tctctggaaa aaagccagtg aacgtttcta caacttggga
     3961 aatcctgaca aattctttgc tgatcgagat gcaagcgaat gggtcagctt taccctgacg
     4021 acgaaaaacc atttgttgtt gaatgaaatc acccgtatca cgactcaaga accaggaaat
     4081 gccttgaatt catttatttc aaccacaccg atcacgccgt taggccaaaa agatgtcaat
     4141 atgtctatcg tcgtgcatca tcaaccgcat tttacgtctc agaaatcaaa tgaaacagtt
     4201 atttggggtt atttccttta tccaagacga cgtggcgaat ttgtggataa acagtatatt
     4261 aaaatgaatg gaaaagaaat gctagaagaa ctgatcggac aactttcaaa agtagatcct
     4321 ggaccagtga atattcgtga aaaagaaaca gaaattttcg acagcgtgat aaataatatc
     4381 cctgtctata tgccttatgc ttccgcgttg ttcaataatc gtgccaaaag cgatcggcca
     4441 aaagtcattc ctaaacattc gaccaatctg gcctttacag gagaatttgt cgagcagcct
     4501 tatcagatga tctttactga acaaagtgct gtacgttctg gagaaatcgc tgctttccat
     4561 tttgcgggta tccctatgtc ccgtcttgtc aaaacaccgc gttacgacaa agatattcct
     4621 actctagctc gtgccgcaaa aaaaatgttt gaataaccaa gaggttgtga aaaagctgac
     4681 ctgcatcaag taataagaac aaccaggcta aaacagcttc ttatgttttt gatttggttg
     4741 gacttattac cgcagatgca agcttttgaa caccgtttac caagaggttg tgaaaaagct
     4801 gacctgcatc aagtaataag aacacccaag ctaaaacagc ttcttatgtt tttgacttgg
     4861 ttggacttat taccgcagat gcaagctttt gaacaccgtt taccaagagg ttatgaaaaa
     4921 gctgacctgc atcaagtaat aagaacaccc aagctaaaac agcttcttat gtttttgact
     4981 tggttagact tattaccgca gatgcaagct tttgaacacc gtttaccaag aagttatgaa
     5041 aaaggcgttt agtttcgagt atgaagaaaa attttgtgaa aatggttcct cacgtttttg
     5101 cataaatttg acttaatacc gagaaactga tcccttctgt cataacctct ttttatttat
     5161 gtctgtttct ttttgaaaac aaccattttt tcaacgttta ctctcttgcc aatccaacat
     5221 catctaacgg atacaactat ttcaatgatt accattacac taaaaaatcg gaaacacacc
     5281 tctccgaaca caaatacata agactataga atgtttcatt taagaaaaaa agcgtttcat
     5341 tatcaaaaag gtaagatctc ctttaaaatg aaattatctg tagtactaat aatgcagaca
     5401 attttatgaa tttgaaagga gcatctccaa tgaaagcagt cacttggcaa ggaaaacaga
     5461 aaatggaaat cagaaacgta gatgatccaa aaatattaga accaacagat gcaatcatcc
     5521 gtatcacagc aacagccatt tgcggctccg atctgcatct ttaccatcat ggtgaagcag
     5581 ttttggaaaa agggtatgtt gtaggacacg aaccaatggg aatcgttgaa gaagtgggat
     5641 ctcaagttca aacattgaaa aaagggcaac gtgtggttat cccttttaat attagttgtg
     5701 gtcattgtca tttctgcctg aatcacatgg aaagccaatg cgatgaatca aatccagatc
     5761 agttatatgg cgggcttttc ggatttggca agttgaacgg taatcattgg ggtggtcaag
     5821 cggaatatct aagagttcct tatgcagact ctacttcatt tgttgtacca gataatgttc
     5881 ttcctgatga aaaagtttta tttctatcag atattttgcc cacagcttat tggagcgttg
     5941 aaaacgcagg agtaaaaaaa ggcgatacag ttgtgattct aggttctggt ccagtcggtc
     6001 tattcgctca aaaattcgct gttatggctg gagcagagcg cgtgatcgca gtagatccag
     6061 tacagcaccg tctagataaa gcggctaatt acaatggggt tgaaacttat ttcttagaag
     6121 acacggcaca agctggaaca gaattgtatg aactggctaa aggtggcgca gatgtcatta
     6181 tcgattgtgt aggaatggac ggattagagc ctgtcaaaga aaaagcgaaa aatttagtaa
     6241 gcttacaaag cggcacaatc tctccattac agatggcatc gcaagctgtc aaaaaatttg
     6301 gaacagtcca agtaactggt gtttatatga ctccagcttc ttcttacccg ctgcaagaat
     6361 tttttatgcg ggatatcgct gtcaagcatg gacaggcacc aattatccat ctaatgccaa
     6421 aaatttacga aatgatcgca gaaggactat tcgatcccac acaaatcatt actcattcgc
     6481 tgccactatc agaagcagca caagcatatg aaatctttga caaaaaagaa gataaaaata
     6541 taaaagttgt tttgaaacct tgaattcagt aaaaaataca ggtgattttc tttatgccca
     6601 aaatcaaacg aaatacccat gagatcgaaa taggaaaaga agaagatatc gaaatacgag
     6661 gacgtcgctt ccgcttctat ttccaaaatc gttacacatt gatctcactg gctgtcgatt
     6721 tattaaccgg gattttctat atttttggaa gccttgcttc cttaactaaa atccccgatc
     6781 gatatggcac ctatttttat ttggctggtg ctattttctt gacaattcgt cctattctca
     6841 gaatcgtaag gaacattttt atttacgacg aaaaaaaaca atgaagagaa caagcaaaaa
     6901 aaagaggcat gagatttgaa aaaatctcat gcctcttttt taaccctatc gtatcacttt
     6961 gatacagacg agcggtcgtc ccactttctt ccttgaccat tgtcaaaaat gtccactcgt
     7021 atcgttcata aaacgtggta tgatctgtaa ttaaataaag tttttcatac ccaagttgat
     7081 gcattttttc taatttccgc taatatccgc ttggctatcc cctgcttacg cttttccggt
     7141 tcaacaaaca gggcacaaag atttggtgtc agatcgggac ggttatggaa gtcgttttca
     7201 atgacgcccg ctcctccgac gatggtttct ttttcatcta agaccacata ccattgagga
     7261 atactttcct tattagcgat tccagtacga atactttcct catacacaac agcaggaact
     7321 ccccattttg atgaaaacca cttcgcactt tgttttacta attctgggtg ttctcttaat
     7381 ttgatccagt tatatgtaac caatgtttaa acctctgact aaaataaaac gtttacaaaa
     7441 attatacaag tctttatgaa aattagcaat gacaacaata gtgatgtaaa aaaaaacgga
     7501 aaacaatcac ttgttctccg ttttttgatt aatcaagcat ctaaagcttc ttttcttctt
     7561 tcttggatcg tagctacctt gacgaacggt agatagatca aaatagaaag aaccaaattg
     7621 acagcggcta gtacaccacc tgcaatactt tttgttgcca atacaccgcc aataattggt
     7681 ggagttaccc atggaggcat aacggtagct gctggcacta aacctgtaga agtcgcaaag
     7741 taagcaacac taactaagac cattggtgtc aaaataaatg gaatgaacat gatcgggttc
     7801 aacacgatag gtaacccaaa catcgttggt tcattaatat tgaagacacc tggcgcaatc
     7861 gacaagtttg ttacaaccat atatggtttg tttttacgtc ctactaaata aatagctaac
     7921 agtaaaccta aagttgcacc tgttcctcct aagttgacaa agctgtcaaa gaaaggtttg
     7981 ttcacgataa acggtgcgct atgtccagct gcgattgcat taacgtttgc ttcaatggct
     8041 ggcgcattga ttgtttgcat caaaggatcg accatattcg ctccgtgtaa accaaagaac
     8101 cataagaatg gtgtgataaa tgcaagcagt aaggcagatg gataagagtt tgcaagtccc
     8161 ataaatggtt cttggaccac tgtatagaaa gaacctacga tatcatcaac accgaatgca
     8221 gcaaaaattg cagcaactaa agcaaacgca gagatagtga tcattgctgg tagtaatgaa
     8281 gcaaatgctt ttgcaactgc cggtggcaca ccatcaggca ttttgatgat caatttttca
     8341 tttccgctta aacgtacaaa gatttctgtt gaaataatcc caacaataat agcaataaac
     8401 aaaccgtttg agctcatacc taatgctccg cctaaagcga agaaagagcc taaagaaatc
     8461 acacctgcgg aaacaccgtc ttttcggtag ccgttagcta aatgataagc aagcaagaaa
     8521 gcaatcaaga cagaaagaat ggcaaatgta ccgttccaag cagcagcccc gaatcctttc
     8581 catgattcac caccaaaaat tgagttcatc aattcttgga acccgggaat cggtaagtta
     8641 ttgatcatta ctgctaatgc tcctaagatc attaacggca tactcaccat aaatgagtcc
     8701 cgaatcgcaa ctaaatgccg ttgtgcacca atttttgatg ctacaggtat aaaatgtttc
     8761 tccataaatg caacaaattt atccataaat cttccctctt ttctttactg tagattacgc
     8821 gttcaatcga cgatacagat caatgatctc ttttgctaaa tctgtaaaag ctatggatgt
     8881 catcagatga tcttgactgt ggactgtcaa aagcgtgact tcaacgtgat ttccttgtgc
     8941 ttcttgtgtc agcatctctg tttgagagtg atgtgcttgg actaaagatt cctctgcatc
     9001 tttgattttt tcatctgcta aagtgaaatc accttttttc gcagcagcaa tcgcttccat
     9061 tgcatcgctt tttgcatttc cgccaaacat gatcagaccc atgatggctt ctagattttt
     9121 ttgatcttcc atttattgta tcctccgata atttttatta tttgtttatt agtttttctg
     9181 cttggtcgag tactttttca ccattcatca tgccatagtc agccatattg attatatcta
     9241 aaggaatgcc tttaggtgct agcttttcag caaattgatt ttgcataaag cgtacttgcg
     9301 gtcctaaaag taaaacgtct acatttttag attctagttg attatccgca tctgaagcag
     9361 atacagcaaa gatgtctgct tccattcctt ttgcttctgc tgccttttgc atttttgtga
     9421 ctaggagact tgtactcatt ccagctgaac atacaagcat gattgttttc ttttccattt
     9481 taaacacgtt cctttctata cttacacttg tatataagca agtagcgtgc caactttaaa
     9541 agcgctttaa aaagttgata tcatcacatt taaggtctat accaagttgc atacacacta
     9601 aaaaagaaag tgcacactaa acacacaaat taatttttat tacctttttt cttttaacgt
     9661 gcacaaaaaa agttgtgaca caaaacttgt cacaactttt tttcgaataa acggtattca
     9721 aaagcttgca tctgcggtaa taagtccaac caagttaaaa acataagatg ttgtttttac
     9781 tttgctattc ttattacttg atgcaggaca gctttttcac aacctctcaa taaacggtgt
     9841 tcaatcagct gaccctggat tttagctcaa aacgctggat tcacaacctc ttttatttca
     9901 aataaccagt aaatacaatg aacatctaca aaaaacttct atttatttat agatttcgtc
     9961 aggcaaatcc aattctctct tatcaatcaa agtctgatac cacgaagcag attttttcaa
    10021 tgttctgctt cgttcatcgt tcaattcgat tcgaactaat ccatatcgat tcttaaatgc
    10081 attcattggt gacacacagt cagtgaatgc ccataacata tagccttcgc aatttgcgcc
    10141 ttcttctgtt gctttgatca aataacgcaa atgctgtccg ataaattcga tacggtaatc
    10201 atcttgcacg attcctttct catccatata ccgttcttca tgttctcttc ccataccgtt
    10261 ttctgttatc agccaaggga tattatgata attttctttt agatacatgg ctatatcata
    10321 catgattttc ggatagattt cccatcccct ggaattattc attttcttcc caggtaaatc
    10381 aaagacttca taatatcgtt ctggatggaa aggtgtctct tcattccatt gataacgagg
    10441 agcgcgaacg cgtttaggat aatattgatt gattccaagg aaatctactg tatgatgctg
    10501 aatcgtttct aaatcctctg ttgcaggatc gaaataaatc gattgttttt gacacaactc
    10561 caggaactct tctgtgtagt acccttttac catcggatca aaaaatactc gattataaaa
    10621 caaatcatac atataagcag ctttttggtc ttctggagaa ttagatcgag catagaccat
    10681 ttcaggattt aaaatacagc caatccttcc accataattt ccgctgcggt aaatctctac
    10741 tgctttagcc gttgctaaaa ctttatgata attccacatc atccactttt ttgtgttttg
    10801 ttcatgcggc cagcgaacag catccaaata gcaacgcgtt tggggaacga ttggttcgtt
    10861 gaatgtaaac cagtttttta ccctatcacc gtaacgatca aaggcaattt gagcatactt
    10921 agcataaaaa tcaactactt ttcttgaaga ccagccatca tatttttcca aaagaaaagc
    10981 tgggacttca taatgttcca aacaaatcat cggttctacg tctgcttcga tcaaagagtc
    11041 gatcatttca cttatatgct gagcatattc ttcatctact atacagttct cataatcagt
    11101 aaaaaaacga gcccaattga tagaagtccg ataatgtgtc agatggatgg atttcatcag
    11161 atccacatcc tgctggtatt gttccataaa attagttgct acggctgggc catacccatt
    11221 atgccaaaca aaatgttcat ttttatacca gctatctata aacgaatcct gtccttcttt
    11281 ttttcctttc caaccttccg tttgccaagc agaagatgca gcaccaagaa taaaattttt
    11341 tggaatagta attttcatga tttcctcctg tttttagatc ccatcatttt ccacacgatt
    11401 tgctactttt acaaatggca gatagatcaa aatagaaaca ataatacaaa caagttgcgt
    11461 aataactgca ccgatatttc ctgctgttgc cagataagca ttgacgatcg gcggcattgg
    11521 ccaagggacc attacaactg ctttcgccgc aaaccctaaa gcggttgcaa aataaccaat
    11581 cgttcctgtg ataagaggcg ttaagataaa cggaatagct aaaattggat tcagcatgat
    11641 cggcatacca aaaataactg gttcattaat gttgaagatc ccaggagcaa gggataactt
    11701 ggtgatttgt ttcatatcct cccgttttcc aactaacaat actgcaatca gtaaaccgat
    11761 cgtgactcca gacccaccca tactcatata catatcccaa aatggcatag taataatgtt
    11821 cggtacttct tttccagctt caaaagcttc cgtatttaca gcaattgccg ctaaaagcaa
    11881 cggttcccgt atcggcttca ccatttgatt tccatggata ccaatcaccc aaaagacttg
    11941 cgcaataaac atcaatagca aaatacccgg taacccttga acgacacctt ctaatgggcg
    12001 ttgtacgaca ttgtaaatga tatcataaag gtacattcct gtaagttctt tgatgataaa
    12061 tcccgcagcc gcgatacccg atacagtcag aatcgtcggg aataaagcag aaaaactagc
    12121 agaaacattc gttggcacgg tatcaggcat tctcagctgt agtttcttct gtttcctcaa
    12181 ccagcaataa agttcaatag aaaggatgga gaccaacatt cccaaaaata accctttcgt
    12241 gtctgtatat tgtttagcca atacgttttc tactataaca gtctgatttt cattcactaa
    12301 ttcataaaca gttggattaa cagataaata agacatcact gcgatgatac cagggaatac
    12361 accatccact ttattcagct tggctaattc gatccctatt agaaaaacag catagatggt
    12421 caagaaactt agtgtgacat agctgatggc agatgcgata ggctttaatt cttctaaaaa
    12481 acgcaagtaa cttatctgtg ctaagccatt ttccgagtca aagaccattg ctgaaaacaa
    12541 tgtgccaaaa gcacccgtaa taattaccgg aatcaatgca gcaaatgcgt tttttatcac
    12601 catgatatac cttaaattat tgattctcgt tgccacgttc cctaaaactt tggctaattt
    12661 atccataaaa ctcattccag atcccctcat tcctgcttct tctccttgac gtcatctctt
    12721 tttgtctatt agtcagcgct tacattttgg tgaacagaaa ctatttactc ctctttttta
    12781 ccaaatataa aacaaaaaat catctgatgt atttatcata aaatacatca gatgaactgt
    12841 caattagttt ctttttctcc tattattttc tgtaattctc tccgatttat tgccatatgg
    12901 acagtcattg cgtcaaacgc agcaagtacg ttccgccttt cgatggcctg aacgatctct
    12961 gtatgaatat cgatcgtctc gattttcaga tgctggatat cataattttt attaaacaaa
    13021 gcaatcgatt cattaatgat tgggataata tgcagcaagg ctatgtttcc gctagcttct
    13081 gcaatcatcg tatgaaaatc gatgtctaat tgaacatgtt cttgtgtgcc ttctcgaaaa
    13141 gaagtttcaa tcttttgcgt taattttttc atttggacga tttgattctt cgttgcatat
    13201 tccgctgcca aacctgccat cttaggctcc aataaatatc tcagttcaaa taagtctcgt
    13261 atcatccgat cctgatcttc aatcaaagaa aaaccgagtg ggtcattggc aatccctcgc
    13321 ttatcactga caaatgtccc cgctccttgc cgaacttcaa ggatattcct ggaagctaga
    13381 gcccgcacag cttctcgcac cgtgcttcgc cccacctcta aataatcgga caattcatat
    13441 tcattcggca gtttatctcc tacttgcaac tgcatttcct ttaaatatct aaggatcttg
    13501 tcagtagtta cttctactag ggttttattt tcttccaaac cacatacctc cttttcatct
    13561 gatgtatttt ttcgtaatta ttataacacg tttgaatcat ctgatgattt tttgtataaa
    13621 aaatcgatta atccgttcat agacatacaa acgaagaaga tatttaccat aaaaaagact
    13681 aggataaaat tcactcctag ccttcgatca tttttatttt gatcgacaaa aaatatgatg
    13741 ttcaataaca gacgatcttc attcgattct tgatgtcatc ttttgttttt tttaatatat
    13801 ggataaaaag tttgattaca tcgtttctat tctagaattt ggtcgattct caattcttct
    13861 tctggtgaga aatctaaatt atccatcatt cgaacacttt cttgtaactg acttaaccga
    13921 cttgctccga tcagtacact tgtaacagat ttttgtcgta aattccatgc aagggctatt
    13981 tgcgctaatg attgtcctcg tgatacggcg attgtttgca gtgctttgat tttttccaga
    14041 gttgatccaa cttgttcttc tgaaaggaat ggtatttcct tacgatgtgc tctagaatct
    14101 tcaggaatac catgtagata acgatttgtc agcaaccctt gagcaagcgg actgaacgtg
    14161 attgccccta gcccttcttc ttctaatact ggagatagat cgtcttctag tagtgcacga
    14221 gaaaacatat tgtagcgcat ctgatgaata atgaacggtg cttcctttct ttttaaaatt
    14281 tcagtcattt ttttgtatct tctccattat agttggatat accaatatat aatgcttttc
    14341 cttgtcttac aagttgcatc aacgcttctg ctgtttcttc caaaggtgta tcagggtctg
    14401 ggcgatgatg atagaagatg tcaacataat ctagccccat tctttggaaa ctttggtcac
    14461 aacttgatat gatggattta cgagagcccc attccccata tggcccttcc cacatatgat
    14521 aacctgcttt gctagaaata atcagttcat cacggtaggc ttgaaaatct tctctaaaaa
    14581 tacgcccaaa gtttttctct gcagctccag cagggcctcc ataattattc gcaaggtcga
    14641 aatgggtaat tcccatgtcg aatgcgcctc ttaaaatctc tctttgatta tggataggat
    14701 cataatcacc aaaattttgc cataatccga gaggagagat aaaatcggta atttcaaacc
    14761 tgaatgaccg gatttacgat attccatttt ttcataacgt gcactatctg caatatacaa
    14821 tttttctcct cctcatcaaa tctttttaaa acgctttctt gttttctatt tttagtttac
    14881 tacattttaa agaaaaggag ttaatcaaaa tgattttttc gtatattttt ttgccatttg
    14941 ttttttactc gttgatagat cagaaattga ttacgtgctt gctggatttt tttatagcgt
    15001 ttcggttgtt ttttgtaaaa ttgctgatga tagttttctg ctggccaaaa cgtaccggct
    15061 ggttcgattt ttgtgacgat tggttggtgg aaagttcctg aactgtctag tttctgtttt
    15121 gattgttccg ctaattcttt ttgtcgttcg tcttgataaa aaatcactgg acgatattgc
    15181 ttccctctat cttgaaattg tccaaatgta tctgtgggat cagtaatttg ccaatagatt
    15241 gtcagcaact cgctataact tatttctctt gtgtcataaa tgatctccac agcttccaca
    15301 tgacctgtgt aaccaccact tacttgatca taattcggtt tttcaacatg tccgcccgtg
    15361 tatcctgaca aaacagatag gatccctttt ttcgattcaa acggctcgac catacaccag
    15421 aaacatcccc ctgcaaatac tgcacgttct tgatagtcag catcttgcat ggcatgcttg
    15481 gaaaagtcat atttttgctc cttatcaaat ccttccgtaa tcaataaata aaagtccatt
    15541 acatcaggcg tcagattcat tcggatagct aatggacgta gtgttacttc tagttttgat
    15601 aactgttctt taacggatat attttctctt gtgtcattct ttgtctgaat caatactttt
    15661 cgttcccaat ctcgtgtatt cggattcaaa atcagattat aaagtgtttg aatgaatatt
    15721 tgttcctcat ccatagattc tcctcctttc cgaagaagac aagctagtga ctttagctgt
    15781 aatttatcat aaagtgagga aataatcgat tgaatcgtat ttattcaaag aagataagca
    15841 gcttcagatg atataagatg atctttatct taaaaagcat actctatggt ttggtttaac
    15901 taaaaaacga cctctgaaaa aagggatgtt ctccaattct cagaagtcga gtaaagatga
    15961 ttctttttta tgagcgataa taaacttcat ccaaaaacag accttgtgca gggacggttt
    16021 ctcctgcatg ttgccgcact ttgctttcaa atatttcatc gattgtttct atcggcatgg
    16081 cacctgtgcc gatttctagt aatgtcccca tgataatccg caccatttta tataagaatc
    16141 cgtctccgac aaaagtaaaa tgaagcattt ctccctttcg ttcgatagat aattcatcaa
    16201 tcgtacgcac tgtggatttt tttgattttt tcaatgcaga aaaaccaatg aaatcatgtg
    16261 tacccactaa tttttcgcaa gctgcattca gcttgtccat atccaactct tgagggaaat
    16321 aaaagctgtg gtttcgctca aatgctgaag gaatcacgtt gttccaaaca taatagctgt
    16381 attttttgcc caccgcatta tatcttgaat ggaagcgctc aggcatttct tcgattcttt
    16441 tgatgacaat atcccgcgga aggtaacgat tcataaagtc gatcatttct gccagtccaa
    16501 tttctgtgtt tgttttaaaa tttgctactt gtcctcgcgc atgggtgcca gcatctgttc
    16561 ttcctgatcc aataatttct atctcttcat ttgtcatctg atgcaatact ttttcgattt
    16621 ttccttgaat ggttttatcg tcatctccaa gtctttgcca tcctgaatat ctttttccgt
    16681 catattcgat tgtcaacttg atgtttctca tattttagtg gctcctctgt cttccatttg
    16741 gtcttctctc taccttttta agattgtagc acaaaatata gctccttgct tctctaagac
    16801 agaagctata caagaaaact gtggaaatat atatttcaga aaaacaaaaa acagccaccg
    16861 gatgaagtat ccgctgactg ttcaatttga cggctcaagt tttactgttt gagatgcaag
    16921 atactcaacc aggcaatggg cacataatta aagaaaaaac tgattctttg gcaaaaacct
    16981 attttattca gtaatggcca agaatttata gaaggaattc ttgccagtcc ataaatcgcc
    17041 gcaaaaagca aactgatcac agacagtcca aggtataagt ggcttacgtc catttgccca
    17101 ttttttcgat agatcaagaa aagaagaact ggaaacaata aaaaaccact gtaacctaaa
    17161 cctgatccga tattatggac ccatgtcgag acattccacg aagattcatg ggtattgata
    17221 ctgaacaaac ctgtaaagat acaatcacct ataccataga acgcgatggc tgctgtaact
    17281 agggtagcta acacacgaga tgtacttaca aatgcagcat acaatgcagg caatgacagg
    17341 atgaaaaaga tccctgaaac cacagaccaa ataagaaacg cttttcttac aggactggaa
    17401 acctctccaa atacactgat cacgctcttc atttgatcta attcaggata atacaatcct
    17461 aatacgtacg gagtcaaaaa atcactcagc acgcctaata ataaaaagaa aaaaccaaat
    17521 ttacgtaaaa aatccatgat acgcctccaa taacaattac ctatgctttt aattgttatt
    17581 ataaacgact ttatcttgat ttaacagaaa cctctattta cacgaacatc tatataactc
    17641 attaaaaact cgaatcattt taaaaatact aaatatctaa cgttcaacgt tcacttaatc
    17701 catctaaaca atctttcaat ttgatttttt ccccacttct tttacgcgcg cctagctctc
    17761 ctattgcagt aaacaacaga tcacttgacg aatttaaggc agtttccatt gaatcctgga
    17821 taacaccgat aataaatcca ataccaacga tttgcatcgc gacatcatta gagataccaa
    17881 acaaactaca tgctaatgga atcaataaca gtgaaccgcc cgcaacacct gaagctccac
    17941 aagcagctaa agcagacaaa atacataaaa ggactttcaa aacaaatggt gcttcaatcc
    18001 ctaaagaatg tacagcagtc aacgtcatca aggaaatggt aatcgcggca ccgcccatat
    18061 tgatcgtagc acctaacggg atcgatatgg aataagactc ttttgtcaat ttcaatcttt
    18121 cagccaacat catgtttatc gggatatttg cggcagaact tcgggtaaag aaagcgggaa
    18181 gtgcgctttc tttcaaacaa aagaaaatca atggataggg attttcttta ataaacaaaa
    18241 acgtgaggaa tgggtacaca actaatgcga caaacagcat cgtaccgatc aaaacaagta
    18301 aaagctgcag atactctgcc aatccagcga ttccagtcgt agcaattgat tgataaacca
    18361 atcctaaaat ccctatcgga gcaaatgcaa taatcacttg tacgacttgt gacaacgcta
    18421 tagatatctg atccaccaac tcttttgtcg attctgaacg tgctcgaaaa gctagtccca
    18481 acaatacagc ccagaataaa acgccaaggt aattcgcatc aataagagat tgaacaggat
    18541 tttgtgtgac attcgtcaac atagtagata aaacactttc caaactttgc ggtgcttttt
    18601 cagatactgt ttcttgtaaa gtaataggaa cagtaaaaat tgaacttaca atgactgcta
    18661 caagtgcaga aagcaatgtt cccactaaat ataagatcaa aataggcttc acgaatgtct
    18721 tattcccaat tttatgcttg gcaattgacg ccatcgtcaa gaaaaaaacc agaataggtg
    18781 cgattgcctt caagcaactg acaaacaacg tccccaatat tgaaataaat gtccacgtgg
    18841 ggaaaaagat accaaacaat attccgacta aaataccaac aacaatacgt tgaactaacg
    18901 acatatccaa aaacttctct aacaaatgac caccctttca caattttaac aatcattgta
    18961 tcattttatc taaagaaact aaaggtgcaa ttttctaaaa attctaataa tacaattggc
    19021 aattcatgct cttgcaacat tcaattttag tattggaggg tttattcaat ctctaaagaa
    19081 gtcgagagaa aactatttat cgttattatt atagttatcc aagtaaaacg taactaagat
    19141 gaaggacgaa actagaaatt tcatccttca tcttaattct tagacatagc aatcaattaa
    19201 tattttccac tgagtctctt ctttctctca ctctcactgg tttacttacc cataaacgat
    19261 ttgattaaag ccatttttca attcattatt cgctgtctcg ttaaagtaaa gataattatc
    19321 gggattcccc tgattttgtt gtgtcaccaa tacttttggc tgattttgct gaaacccgaa
    19381 tagatagacg ttctttttca gatatggttc tgtctctgca tcggaataga cggctgtgat
    19441 ttgatagtct gcttgacctg tcccactttc tgaccattct gcagaaacag gcactccacc
    19501 aatagccatc ttccactccc cgttcaaaat aacttgaggg actttaagac catataagtc
    19561 cacactcatt tgattcgtat agcttttgta ttgttgtccc attgtttggc cccatgtagc
    19621 cataaatgcc tctaattgtg cggatttgtc tgcattccac agtgtttgtt cgacttgttc
    19681 gctgctttct gaagatgaag cctctacaga agaacttgaa gtagatgttt gagttgtttc
    19741 agtcgttttg gaagactctg acgaagtaga ggtatctgct gtttccgtac ttgctgcagt
    19801 tggcttgctc tctgtgctag ttgttgtagt agttgcaatt gttgctttcg ctgtttcttt
    19861 tgttgattct tttttcgatt cattcccgtt ggtacaacca gctaacaaaa aagctagacc
    19921 aattgttact aaaatccttt ttttaaatta aaaaactcct ttcgattact cccacttctt
    19981 tattctatca aataagcaat gtgattttat tgattttttg tattttctca tccatctttt
    20041 aaacctttta aaaactaaga agcacccgct gatgttgtct gtaatacatc agcgggtgct
    20101 tcttttattt taatcttcgt gattttccaa tttacctcac gctttatatt tgtatttgaa
    20161 gaaacataga cttttagtca tcatcgtctt cctcatccaa atcttgtcgg acagtttttc
    20221 cttggagtct ttgtacttgg tagatgattt ctttattgaa acttaaaagt tcaacaagca
    20281 attgtttcaa gccatatttt tcttcttttt cagctaattt gattgcccgt gtgataaaga
    20341 cattttggaa atcaaaatct tttgcaaagt tttccaacat tgttgaggct ggtaaatatt
    20401 tgttttgacc atgttcgtaa atttttccat aacgcatgac ttcttctaaa gtagacggaa
    20461 cgatttcatt ttcaatcact aactcatgag agattttatt gaaccatctc gcttcttgat
    20521 cagcatattc tgaaaaaagg gtacttaatt cttgcgaaga tgtacccaaa acatacaatt
    20581 gtgcttggat caatttcatt tcttctaatt taaaatttgc caatacatgc cccaccatgg
    20641 cgccagcagt tggtttatga tgatcgatat ctgcttgttt tgtttcttga gcaaattttt
    20701 cttccactat attcactgat ttacgcctct tttacttttg ttgattggac ttcataacct
    20761 aatttttcca caactgcagc taattgttca gggtggactt catctggatc aaagtttgct
    20821 ttgattttgc tcgcattaaa caaaactttc gtattgctta ccccaggctg ttgagaaacc
    20881 gcatgttcta ttttttgcat acagcttgga caagctagtg tatctaattg cataatcatt
    20941 tttttcatcg tagttcttcc ttcctttttc ttgatagtct tattctagat tacagcttcc
    21001 ataaaaaaat tgattcttat caagttttac gatttttctt ctttgctttc gtgtccaaaa
    21061 tacaataaac gcatcgcgtt aaagataacg atcaagatac tagcttcgtg aacgaacatc
    21121 ccactagcca tgtaaataaa cccggcaaat aagccgatga gtaaaaataa gacgacacca
    21181 atggcaatca ctacattttc acgggtattc aaaacagttt tcttagccaa acggtaagca
    21241 tgaaccaacg atccaaaact agattgcatc aagacaacgt cactcgtttc gatagcgaca
    21301 tccgttcctg atcccatagc aatcccgata tcagccgcag ccaaagaggg agaatcattg
    21361 attccatctc caatgaaagc aacgtgcaat ccttgttctt ggtatttttt cacaaattga
    21421 accttttcat ctggcaataa ttcagcatgg acttcatcaa tgcctaacat atcagctaca
    21481 taatcagcag tcatctgatt atcacctgtc agcatcacta aatgttttgc tcctgcttgt
    21541 ttcaacatag ctaattgttc agcgacttct ggtcgaatca catctgacac accaatgatt
    21601 tgagtgattt ttttgtcgat tgctacaata atgactgagc tgcctctttg ttgtaattga
    21661 tgtaaatctt gtacttgctt tggtgtgaga gaaagtttgt gagcggcaat caatttggtg
    21721 ttgcccgcac aaactgtatg accatcaatc tcagctgtga ttccttgtcc cttgatcgtc
    21781 tgattatctt tgactgctaa ttgttgaaaa tcaatgtctt tctcctgagc ataatggatc
    21841 acggcgcgcc ccagaggatg atcggataac gtttctaatc gagccactaa agctaagctg
    21901 tccccttcat cctctgtata gttcttgata gtggatacag cagtagttcc tttggtcaat
    21961 gtaccggttt tatcaaagac aaaagtatcg attttggaaa aagtatccat tgcttcaccg
    22021 cctttgacta agatgccccg tttcgctcca ttaccgattc ctgctacatt cgaaacaggc
    22081 gcaccaatga ctaaagcgcc aggacaacca agaaccaaaa cagtgatggc taaacgaaaa
    22141 tcacgactga tcagaccaac aagcaacgca ataagtaaaa cggttggagt ataatactga
    22201 gaaaattgat caatgaattt ttccgcatgt gatttggtat cttgtgcttc ttcgactaat
    22261 tctataattt tggcaaaggt cgtatcatct ccgaccttag ttgcttcgat cgtcacgtag
    22321 ccatctgcta aaatcgttcc agaaaatacg ttatctccac tgattttgac gacttgtcgt
    22381 gattcacccg tcactgcggc ttcatccgca taccctttcc cgtcaatgac tcgtccgtcc
    22441 acaggaatag ctccaccagt ttttactaag acgatatcgc cttcttccac gtcatcgatt
    22501 tccacttctt cggtcgttcc atcttcttgg acaactctgg cagtcgatgg ggccatctcg
    22561 gtcaaagcct tgattgattc ccgcgttttg gtcaatgttt tttgttccag ataactacca
    22621 aacaaaaaca agaatgtaac gatagctgac tccttgtatt ctccgatcat aaaggcacca
    22681 agcacagcaa tagtaacgag taaatcaatt gagataactt tgacttttac tgcctgatac
    22741 gcatggatta taataggtat gaccgcaata atagaagcta tggctagagc aatgttatag
    22801 aacaagacaa atccagtaaa tttcccgata aaaccaaaaa taatcaacac agctgataag
    22861 gcggtgatat gatttcgatg ctcttggatc cacttttgta atttcatctt tctccctctt
    22921 ttccttttca ttcttacgag actaagtata gatacttatc catcataaaa aattgaccac
    22981 tatcaagttt attcaaaaag gcggttttct tttggtatac tgaattccta cagtcattaa
    23041 acgatcatat agaaaaagga agcgccgaaa cgcttcctta ggtagttcta tttttttatt
    23101 caaagataag atgctttgtc atcttacgac tgaagaacaa caatcaaaaa agggcagcag
    23161 ccactcattc acaagaaata tcgttttttg atatgggaat tgaagcagtg gacaaatatc
    23221 tctacttatt agctgggaat aagatccaaa aaagcttaat ggattttatc caagaattgg
    23281 aatgtacttt ccacaaaaaa ttcactcatt ccattctttt aaaacttttg attcatacag
    23341 cgtgtctaat tgagcgtaca ttgataaacg gtcatgaact taaaattata tcagaagatg
    23401 acacaagacc ttctcatgaa acaatttttc atgtaaaaaa ggccttcaaa aatattgaaa
    23461 cagaatttgg gatcactgtt tcttatgatg aatgtttctt tatctatgat attatcgcat
    23521 ctaaataatc tttttttatg taaacgagta tttttttaac ttattgttta agagtgatac
    23581 gcgaattgta aaattaagtt agaaaaataa aaaggcattt atggtacact caaattgtat
    23641 ttccgacgaa agaaaacaaa ggagtgt
//
LOCUS       DAMEET010000043        23569 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.043, whole genome shotgun sequence.
ACCESSION   DAMEET010000043 DAMEET010000000
VERSION     DAMEET010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.043"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(458..1087)
                     /gene="udk"
                     /locus_tag="PB780_001488"
     CDS             complement(458..1087)
                     /gene="udk"
                     /locus_tag="PB780_001488"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDE0733545.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1171..1866)
                     /locus_tag="PB780_001489"
     CDS             complement(1171..1866)
                     /locus_tag="PB780_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HDE0733546.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1879..2595)
                     /locus_tag="PB780_001490"
     CDS             complement(1879..2595)
                     /locus_tag="PB780_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HDE0733547.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2925..3530
                     /locus_tag="PB780_001491"
     CDS             2925..3530
                     /locus_tag="PB780_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HDE0733548.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3855..4178
                     /locus_tag="PB780_001492"
     CDS             3855..4178
                     /locus_tag="PB780_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HDE0733549.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4360..5730
                     /locus_tag="PB780_001493"
     CDS             4360..5730
                     /locus_tag="PB780_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDE0733550.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5860..7161)
                     /locus_tag="PB780_001494"
     CDS             complement(5860..7161)
                     /locus_tag="PB780_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDE0733551.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7391..9349)
                     /locus_tag="PB780_001495"
     CDS             complement(7391..9349)
                     /locus_tag="PB780_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HDE0733552.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9526..9792)
                     /locus_tag="PB780_001496"
     CDS             complement(9526..9792)
                     /locus_tag="PB780_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDE0733553.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10302..12428)
                     /gene="pnp"
                     /locus_tag="PB780_001497"
     CDS             complement(10302..12428)
                     /gene="pnp"
                     /locus_tag="PB780_001497"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDE0733554.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12616..12885)
                     /gene="rpsO"
                     /locus_tag="PB780_001498"
     CDS             complement(12616..12885)
                     /gene="rpsO"
                     /locus_tag="PB780_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDE0733555.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13136..13699
                     /locus_tag="PB780_001499"
     CDS             13136..13699
                     /locus_tag="PB780_001499"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDE0733556.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13952..14473)
                     /locus_tag="PB780_001500"
     CDS             complement(13952..14473)
                     /locus_tag="PB780_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HDE0733557.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14476..15318)
                     /locus_tag="PB780_001501"
     CDS             complement(14476..15318)
                     /locus_tag="PB780_001501"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HDE0733558.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15311..16036)
                     /locus_tag="PB780_001502"
     CDS             complement(15311..16036)
                     /locus_tag="PB780_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDE0733559.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16212..16508
                     /locus_tag="PB780_001503"
     CDS             16212..16508
                     /locus_tag="PB780_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733560.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17076..18668
                     /locus_tag="PB780_001504"
     CDS             17076..18668
                     /locus_tag="PB780_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDE0733561.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18681..19421
                     /locus_tag="PB780_001505"
     CDS             18681..19421
                     /locus_tag="PB780_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDE0733562.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19589..20938
                     /locus_tag="PB780_001506"
     CDS             19589..20938
                     /locus_tag="PB780_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDE0733563.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21066..21656)
                     /locus_tag="PB780_001507"
     CDS             complement(21066..21656)
                     /locus_tag="PB780_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HDE0733564.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21803..23155
                     /gene="brnQ"
                     /locus_tag="PB780_001508"
     CDS             21803..23155
                     /gene="brnQ"
                     /locus_tag="PB780_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDE0733565.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6749 a         4746 c         4489 g         7585 t
ORIGIN      
        1 ggggtgcgac aaaagagctt cttcaaaaaa ataagtcgaa atctcacaca aattcgagaa
       61 ataattttcg taagtttctt cttatttatt gcatctgacc acttctgtta caacctctcg
      121 ttaaacggtg ttcaaaagct tgcatctacg ataataagcc caacccaacg aaaaacgtga
      181 ggagctgttt tatttggatg ctcttatcgc ttgatgcaaa gacagctttt ttacaacctc
      241 tacttcacgg tgctcaatca gctgaccttc ggtattagct caaaaaacgg taaaatataa
      301 acagctattt tccgtttctt ctttttaata cttaggtcaa gatgctgttt tcacaacctc
      361 ttgttataaa taaaaaaaca gttggctggt gactgttcaa aaagcagaat ccttgctttt
      421 caaacggtcg cagtccgttc aactgtttcg ctgcgcacta tttatggttt aagaaactag
      481 ctacttttgt tgtaatcaaa tcaatagcaa cgtggttttc tccgccttct ggaacaatga
      541 tatccgcata gcgcttcgtt ggttcgataa attgatgata catcggttta acaactgtca
      601 aatattgttc gatcacggag tccaatgtgc gtccccgttc ttccatatcc cgtttgatac
      661 gacgaatgat acgaatgtca tcatctgtat ctacgtatac tttgatatcc atcaagtcac
      721 gcagacgttc gtcttctaaa atcaggattc cttctagaat gatcacttct tttggttcct
      781 gaatgatcgt tgcctgactt cttgtatgcg ctacatagtc atacacgggt ttttcaatgg
      841 ctttatagtt cagtaagtcg cctacatgct gaatcagcag atcattatca aaggcaaatg
      901 ggtgatcgta atttgtgttc aatcgttctt caaaactcaa atggctctga tccttgtaat
      961 aagaatcttg ttcaagcatc atgatggaat ggtccgggaa gttgttgaaa atcgcacggc
     1021 tgacacttgt ctttccgctt ccggatccac ccgtcacacc aatgataatt ggcttgcttt
     1081 ttgtcattga ctaaaacctc tttcgtatcc gttttttcat tctaatctat tatagcgaaa
     1141 aaacacgaaa atgctatatt tttctgaaaa ttaatccttt tcaatttcca ataatgaata
     1201 aagttcctct aatgaatcga ttcgataagt aggctggatt tcttcgattt ctggtaattt
     1261 atttctattg aaccaaatcg tatcgatccc tgccatattt cctcctttga tatcagaagt
     1321 caaagaatca ccaatgatca cagtttgctt tttatcaaaa ttaggaatgt gggaaaagac
     1381 atgatcaaaa aattctttca tcggcttttg cgctccgaca gcttctgaga caaagatatc
     1441 cttgaagtaa tcaagcattc ctgcatctgt taatcgccga tgttgcgtgt gcgacacacc
     1501 gttcgtcaca atataaagat cagcatgcgg atggaccttt ttcaggattt ctagacttcc
     1561 tggaacaaga tcatgacctt ggttcaagta gtggcggtag cgtttttcca tttgatcact
     1621 atctactgtt ttcccaaatt gctggaaaag taacccaaaa cgactgccta ttatttcttc
     1681 tcgtgatagt ctgccttttt caaactcctg ccataaatga tgattcaaag ccttatagct
     1741 ggtttcgatt tctggtgtca atgtgattcc ttgttctgca aacaaagcac ttaaagcatt
     1801 ggcttcagtt aattgaaaat ctaacaatgt atcatctaca tcgaacaata aagttttata
     1861 tttcatcttt ttgacctcct atttaaaata aatacttaac tttttcatct ctgctaatgt
     1921 caacgatcga tactcgccta ctcctagctg ttcatctaat tccaatggtc ccatgcttag
     1981 ccgtttcaat gcggtcactt tctttccttg ggctaagaac atctttttga cttgatggaa
     2041 ttttccttct tggatcgtca ataaaaccgt actttggtgc ttgtctgctt tgagtatttt
     2101 taaatcagcc ggcttacatt gatacccatc agcgaatacg attccatttt caaatgctgt
     2161 tacgtctgcc ggtgtcaccc tttcgttcac aactacttga taagtctttg aaaccttttt
     2221 ttctggaagt agcatttcat atcccaattg tccattcgaa gtgatcagca atagtcctgt
     2281 tgtatctcta tctagtcttc ccactggata taatggttgg aaacgatcgg cacttgcaat
     2341 cagatcgatt actgtcgtct tttctggatc tgatacagct gtaacagctt tttcaggttt
     2401 attgagaaga taataggact catttgtttc taagtgttca cctgctactt cgatttgatg
     2461 cagtaaacta tctacatttc gattttgtcg aaattcgact gtgccatcta tcatcacttg
     2521 cttcaaagca aacagccgct tcatttcctt gcgtgtcgtt tctaattttt cttctattaa
     2581 tttatctaaa cgcatgctgt ttcctttctt attactacta attaattata ccttgaatat
     2641 tcttgcttgc aaatacatca tcatagttat tccttgactt aattaacagt tcacgttacg
     2701 gttaaatagt aaagtgaatt ggatttctca tacacagcgt aatgacctgg tggagaaaag
     2761 ttgaatcctg actttctaag gggagatgcg cgcatctctc cttttgcgct tttttaagcg
     2821 ccctgccttc gatgatgtgc acgagattag cctttagaat tcacttaaat aaaatctgtc
     2881 cgttaccaaa gcagacagtc acattttaag gaggaatttt ggttatgtca gtatcattag
     2941 aagtaagtaa aagagaagta cgtccccgtt cattaagaaa caaacttcgt cacgaaggga
     3001 aagttccagc tgttgtttat gggtataaag tagaaagtac ccctatctca ttcgatggtt
     3061 tgacactaag caaattgttg cgtgaacatg gagcgaacac agtcatcacg atggacatcg
     3121 atggacaaaa agtcaataca ttggttcaca agatccaaac gaacactttt accaatcatt
     3181 tcgaacatgc agaatttcta tctgtaaaca tgtcggaaga aacagaagtc gaaacagaag
     3241 tcgttcttgt tggcgaatca gtgggtgcta aatctggtgg agtgttagct caaaacttgt
     3301 atacagtagt cgtatctgct acaccagaca aattgcctga acgaattgaa gttgacgtga
     3361 caaacttgga attaggtgat tctattacag tagccgatct tccaaaaaac gacgattaca
     3421 aattcgtgac cgacagtgaa gagcaaatcg ctgctgtagt cgctcaaagt gaagaagaaa
     3481 gcacaggtga aggtggcgaa ccagaagtga tcggttcaac agaagaataa tactgaaaca
     3541 atcaggtggt gttagaagcg gcgggtcagc agcttttaac acgccttttt tatttctaat
     3601 cgtttatttt catatggggc ttccactttt gtaaatggct ttacaaacga aaaatcctaa
     3661 tttaccaagc ttttcctcgc atacttttat cgtaccttta taaaaattta tactttgaaa
     3721 aaaagtattc aattattgtt gcatttttcc tgattcttta ttagaatgaa ccctcgttga
     3781 agtaagtatt tctagtttat cctaggatta attataaaaa taaaacattc gttgattcga
     3841 aggaggttaa acagatgttt gaaagttttg atagcagccg caaccgttac gcttcagttg
     3901 gtgtcgtatc aagtttgcca gatgaattga ttgacagtat atggtttatt attgacctag
     3961 atcttaaggg agtcattcca ttagaaaata tattaacctt tgatttgatc gaaaatcgtg
     4021 gaaaagtgac gatgcatttt tctcaagcag gaagcaatgt ggaaatggat attgatttgc
     4081 cgtttgccta ttcgaaccaa tttcctgctc aagtatacgc ctatgatgac ggtacgagag
     4141 aaacgatcct tttaccaagt gagattagac aatattaagg tgatagataa acgtggatgt
     4201 cccttatttt acagaccagg ccaagttgtt gattctttga ctcaattctt ggcttctttt
     4261 tatggagttt cataaattat cattgatttc taattggtat tctcaggata aacgctatat
     4321 actactaact aaattttaaa gatataagga gtttaaaaga tgcgcgattt aacaaaagga
     4381 agccccgcta aactgatttt gatgttcact gttcctttac tgatcggaaa tgtttttcag
     4441 cagttctaca atatgatcga tatgatcatc gtgggtcaga cgctaggtaa aaatgcgctg
     4501 gcagcggttg gtgcaactgg aagcctgaca tttttgatca ttggctttgc ccaaggttta
     4561 acagctggtt tagccattat taccgcacaa cgttacggtg caaaagatta tcggggtctg
     4621 aagaaaagtt ttgctgctag tgttgtcatt agtcttgtcg tcactgtgat tttaactgtc
     4681 ctaagtctcc tgtttattcg tccgatgctt caattgatgc aaactcctcc tgaaatactt
     4741 gatcaagcac aaacatttat ttcaattatt ttattaggga tctttgcatc agtcagtttc
     4801 aatttgcttt ctaatgtcat tcgcgcactt ggtgacagcc gcacaccact attctttctg
     4861 atcattgcgg tcatcatcaa cgtcgtactt gatttgatct ttattatcta ttttggcatg
     4921 ggtgtggaag gtgcagctat tgctacagta atcgcacaag tttcttccag cgtactgtgt
     4981 ctagtgtata tcaagaaaaa aatgcctttg ttacagttac gcaaaaaaga tttcaaattt
     5041 gaaaaagaag agtttgctgt acacctaaat gctgctttgc caatggcgtt tcaatcttcg
     5101 atcatcgctg ttggtgctat cgtgttacaa gcagcactaa atagtctagg gacagatgtc
     5161 gtagctgctc aagccgctgc tggtcgtatc gaccaatttg caaatcagcc gatgatgtca
     5221 tttggtattg caatggcaac tttcactgca caaaactatg gtgcgaaaga atatggccga
     5281 atcttgcaag gggtacgtca aactttactg atgagtgtcg gatttagtct cttagcaggt
     5341 gctatcgtca ttttcttcgg tcatttcttt gttcaattgt ttgttagccc atcagaaaca
     5401 cgtgtatttg aattagcaca aacctacttc aatatcaatg ggtctcttta ctggatctta
     5461 gcagtcctgt tcattttacg ctacacactt caagggcttg gacaaagcaa gatccctacg
     5521 atcgccggta tgatggaatt gcttatgcgt tcttttgcag ctatcgttct tacaggaatg
     5581 tggggatacc caggagcagc agcagcttct cctttagctt ggatcggttc tgttgccgtg
     5641 ttgctttact cttatatgcg ttcgatgaaa caattgaaac acatgggtga agaggaacat
     5701 ttcacattag aacatgcaga agcaagatag ccttgattaa aagaaatcgt tgcggctaaa
     5761 aatgaaaact tttattgaaa atatcttctt cgtttaccga aaacaggttt tttagataac
     5821 aaaaaggctg tgacaaaaaa tgtcacagcc ttttttcttt tattctattt ttctttccgt
     5881 ggactctact ttgaaataat cgatatgtgc atgctggtat tctaataccg tgaaacggaa
     5941 tgatgcaatc tcaatgattg ttcctgcttt tatttcttta tatcggctag aagcaaagcc
     6001 cgcaagtgta gtaaaattcg tttcttcaaa ctctggaatc tctacatgaa aataacgctg
     6061 gaaatcatct aatgttagtt tgccggagat gatataagag ccatctgact gttccgctat
     6121 caatgcttcg tccaaatctg tctcatctcg gatttccccg acgatttctt ctaaaatatc
     6181 ttcaatcgtc actaagccgc ttgtccctcc gtattcatct tttaagatag caatatgctt
     6241 tccctttttt cgcatgatcg tcaataattt ttttataggg atcgtttcag ttgttacgat
     6301 cggctcttca atcaactgtt ctactgtcag ctggtcatct ttataagatt gcttgatcaa
     6361 atctggcaag gtgacataac ctaaaacatt gtcttttgat tctaagataa ccggatagcg
     6421 agtatgtcct tgttcaatcg aagcttttat cgcttctttg acggtttctg aagcttcgaa
     6481 tacttgcatg gacgtacggt ctacttgtat atcttttgcc aaggtatcat caaattcaaa
     6541 cacgttttcc aaataatgat attcttcttt ggtcaactct cctttttcat aagaattcaa
     6601 tgcgactaat gtcagctctt cttgagacaa tgtctcttct ccttcagata cgagacgcac
     6661 acctaatagc ttccctagcc ctgcagctga atgattcaat acccaaataa aaggaaacat
     6721 tactttataa aaataatgaa gtggtttaac tacgaacaag acgaccttct ccgtattgac
     6781 gatactatag ctcttaggaa gcagttcgcc aataaccaca tcgatatacg taactaaaat
     6841 aaatcccagc gccacagaga ttgtactgct aatagccgaa ctaaaaggta gtttaccgat
     6901 cactggttct agtaaagccg cgacagatcc ttcaccgacc cacccgatga tcaaaccggt
     6961 tagcgtaatt cctaattgac tagctgataa atagttatct aaatggtgtg taacatgaag
     7021 agctagcttg gcgttcttta cattttcctt ttctagttgt tccaatcttg atcgtctgat
     7081 tttcactaaa gcaaattcac ttgctacaaa aaatgaagta attgcaatca tcaaaaataa
     7141 aataatgaaa ttgataacca tattctcccg cctttctctt tttctattgt accctttttc
     7201 tacaaaaaga ttgaaaataa tgcgtattca tatttaggaa taaaaaagga gatgcggcat
     7261 ttttcagcca catctcctta tcacttcaaa atcattgcgt tacttgttgc taaacagctt
     7321 tttcatgacc tctgataaac ggtgttcaag tagctgacat tcgacattag ttcaggtcaa
     7381 agcgctactt tcacaaccgc tctttcaacc atgttttccg agtatttcca atgataagcg
     7441 gaacagtttc atgttttact tcgctgtact ctagcccaaa ccatgtttct ggcgaggtag
     7501 cgattttttt gattgccagt tttgcctgca tgatttgacg ttgccatttt cctagagttg
     7561 tcacattgga aactacttca tcaatcagga caaattggaa atcgccaact tctcgccctg
     7621 gtgtcagaga gtatctttgt ggctgttttg gcaaacggcc ttgtttcatc aaatcatgga
     7681 tgacttggcg aacatatacg ttgatttcct gttggaccct gaaacctaag aacagattca
     7741 cttggacaat aaaatcagtg tccatcatat ctacttgata ttctttcgta tatggctcat
     7801 ccgtcacttc cacatgaaca aaccaataga ctcgtgctct tttaggccgt ttatctaaaa
     7861 tcgaatagat gaactgtcgg ccaactttat cttcagccat atcaggaacc aagaagacca
     7921 catttgtctg atagattggt aaagaagtat catcacgtaa ttctctaagc tgcgggatat
     7981 aatctctcaa agacacatcc tcggttgacg attcgcgaat aatgttccct tgttcccaaa
     8041 taaacatcac gactaagatg atcaaaccaa tcagaacggc tacaaaaccg cctcggaaga
     8101 atttgacaac acttgagata aagaaaacga cttctatcgc accgaagaaa aaggtaacta
     8161 aatatgcaag ccaacgagaa tgcccatttt ggataagata aaagtaaagc agtgctgttg
     8221 tcatcaacat cgtcacagtg atcgatagac cgtaagcagc ttccatatga tgagacgttc
     8281 ggaatgtgac aaccactaga gaacaggcga tccataaaat cagattgact gctgggatat
     8341 acatttgccc gatactgttc ccaggataca tgatctttaa ccgaggcagc agtttcaact
     8401 tgattgcttc tgaaactagc gtaaacgaac ctgtaatcaa tgcttgcgaa gcaatgactg
     8461 cagcgactgt tgcaaaagca acgccgatca cgatccaact ttgaggcatc atttggaaga
     8521 atggattcag gttcttgatt tgctgtgctt cagggttttg ataaacattc aacaaccacg
     8581 cagcctgccc aaaataattg ataaccaaac aaattttgac gtaaggccag ctcagacgaa
     8641 tatttcgttt gccgacatgt ccaagatcgg aataaagcgc ctctgcacct gtcgtagcca
     8701 ggaaaatatt tccaagaatg aacaagccta atttgttatc tgcactaaaa agcagctgga
     8761 tagcataata aggattcagt gcacggataa ctgtccagtc ttgactgaag ttcattaatc
     8821 ccattacgcc taaaaaagta aaccaagcga acatgattgg tccgaatgct tttccgacaa
     8881 tatctgtacc aaagcgttgg accatgaata aaacaaaaat aataactaaa gtgataatga
     8941 cgatcacgtt ctggttatta ccgaaccggt caaaaaactg cggaattcct cgcaagcctt
     9001 caatcgcagt cgttaccgtc acagcagggg tcaatacccc atctgctagt aaggcagctc
     9061 ctccgatcat tgcaggaatg atcaaatatt tcccaccttt tcggacaagt gtgtagagtg
     9121 agaaaattcc accttcacca tggttatctg cattcaatgc aatcacgaca tatttgattg
     9181 ttgtcagtag agtcaatgtc caaaaaatca atgagactga tccgataaca aaatcaggat
     9241 tgattcctga caatccgcca ttatcttcaa caatcgattt cataacgtaa agcggacttg
     9301 tcccgatgtc tccgtagacg actcccatcg ctacaagcag gccagccatt gatatctttt
     9361 tggtattgtg cgttgttgcc acgtttttcc ctcatttctt gttatatgga tatcctttag
     9421 gtaagcttga atgatagatg atattcttat ccatgacacc cgctttccta aattgcttct
     9481 aattatccaa gtaacacact gaagtgtcaa cttatttgaa taaaattatt ctttctttgg
     9541 acgttctctg atacgtcttg ccacaaatga ataaagagca aggcaaaaaa ttgcacccaa
     9601 taatgctggt atcaaataaa aaccgccgag ttccggtccc agtggtccta gtaaaaattc
     9661 gccgagccaa ctacctaaaa aaccgataat gatatttcca gatacgcctc ccgggacttc
     9721 ctttcctaaa attgcgccag ctatcaaacc taatataccg ccgataaata acgaccataa
     9781 aaaattcagc atctcctttc cctcctttat ccatatttct cacttttatt ttacgcttta
     9841 ttcagaagga ctccaaaaat atgcgccaag aaactccaga aaaaaagaag ttgtaacaaa
     9901 agtgttcagc ttatttccgt agaagctgct tctggaacac cgtgaatcag aggttgtgaa
     9961 atcagcggtt tgacctgagt attagagaaa caaacggaaa aatagttcct catattttgc
    10021 cgttttttga gctaatatcg agggacagct gattgaacat cgtttattgg agaggttgta
    10081 aaaaagctgt tctgcatcaa gtaataagaa cagccaagta aaaacagctc ctaatgtttt
    10141 taacttggct ggacttatta ccgcagatgc aagcttttga acaccgttta ttcgaaaaaa
    10201 aaggactgtg acacggcttt tgtcacagtc cccttttttc gaataattat tgtccccctg
    10261 tcaccttcac gatcacaagt acattattcc tatgaattaa attatttttc ttcatttttc
    10321 gttgaaccat cttcgttcaa cattgctttt cttgacagat ttacgcgtcc ttgtttatcg
    10381 atttcagtta ctttcacaag gatctcatca cctagtttaa caacatcttc gactttattg
    10441 acacgatcgt ttgctaattg ggaaatatgg acaagtccat cttttccttt gatcaagttg
    10501 acgaatgcac cgaatttttc gatacggaca actttcccta gataaacttc accaactttg
    10561 acttctttgg tcaaatcttc gatgatcttg atagcttttt tgatcatttc tgcatcagct
    10621 gaagcaatac ttacttttcc atcttgatcg atatcgattt tcacaccagt ttcatcgatg
    10681 attccgttga tcgtatcgcc gcctttaccg ataacatctt tgattttagc aggttcgatc
    10741 tggatcattt cgatttttgg tgcatattgg cttaattctt cacgtggagc agcaatcgtt
    10801 gaagtcaatt cttctaagat ttccatacgt gcttgttttg cttgagttaa tgcttcagtt
    10861 aggatttgtt ctgtgattcc ttgaatcttg atatccatct gtaaagcagt gataccatct
    10921 tttgtaccgg caactttaaa gtccatatcg ccaagatgat cttccagccc ttggatatca
    10981 gttaggatcg tatagttttc accatcagaa acaagtccca tcgcaatacc ggcgactggc
    11041 gctttgattg gtacacctgc atccatcaat gctaatgtgc cagcacagat acttgcttgg
    11101 gaagaagaac catttgattc caatacttct gctactaaac ggatcgtata aggaaattct
    11161 tcttctgaag ggattacttg tgctaacgca cgttcaccta atgcgccatg tccgatttcc
    11221 cgacgccctg gagagcctgc acgtcctgta gaaccaacag aaaattgagg gaaattgtag
    11281 tggtggatga atcgtttgct gacttccacc cctaaaccat caatgatttg gtgctctcct
    11341 agaggtgcga gtgtacaagc tgataaagct tgtgtttgtc ctcgtgtgaa caaacctgat
    11401 ccatgtacac gtggcaataa gctgacctct gatgacaatg gacggatttc atccaattta
    11461 cgcccgtcag gacggatctt atcgatggtg atcaattcac gtacgacatc tttttcaaga
    11521 tcttctgtga tttgtttaac ttctttcaac aagtgatctt catcttcatg acctgcaaat
    11581 ttctcagcat aagcttcgcg gatatcgatc ttcacctgct cgatattatc ttcgcgagca
    11641 agtttttctt cagtcattac tgctgctttc attgtagcgt aagaagcatc aaagatttct
    11701 tttttcaata cttcatcaac ttgcagtaaa gtcacttcca ttttttcttt ccctactgct
    11761 tgaacgattt cttcttggaa agcaactaac tctttgatag cgtcaaaacc aaacagcaat
    11821 gcaccaagca tgtcttcttc tgaaacttct ttcgcgccgc tttcaaccat attgatggct
    11881 tgtttcgttc cggcaacagt caattcgata tctgtttgtt ctgcttgttc tactgttgga
    11941 ttcaagacgt actcgccatt tacacgacca acttctacgc ctgcgattgg gccgtcaaat
    12001 gggatatctg agattgatag tgctaaagaa gaacctagca ttgctgccat agctggcgag
    12061 cagtctgttt ctacactcat cacgatgttc gttacttgga cttcattacg gaagccttct
    12121 gcaaacatcg ggcgaattgg acggtcgatc aaacgggcag tcaatgttgc ttccgtactt
    12181 ggacggcctt ctcgtttgat gaaacctcca gggattttcc ctgctgcata cattttttct
    12241 tcataattga tcgttaatgg gaaaaagtct gtatcttttg cttctttaga ggccacagct
    12301 gcacttaaga ctactgtatc tccataacgg actaaaacgg ctccgtttgc ttgttttgct
    12361 agctgaccga cttcgatttg taagggacgg ccgccccatg tcgttttgaa tacttgtttt
    12421 tctgacataa cttctccttt tccttctcgt gaaagcaaga cgctactaaa caaatgaaaa
    12481 ctgccgaaag ccgtcttttg agcagctttc atttggttag taggagtcca tctagcttga
    12541 tcacggaatt ttacttaatt gatatacgag taaaaaaagt gagattcatg tgaatcccac
    12601 tctttttgac taaaattaac gacgcaagcc taaacgtttg atcaattcgc ggtaacgttg
    12661 aacgtcagtt ttacgtaagt aagctaacaa gttacggcgg tgaccaactt ttttcattag
    12721 gccgcggtat gaatgatggt cttttttgtg cacgcgtgca tgttcattca agtggttgat
    12781 ttcttcagtt aatacagcga tttgtacttc tggagaacca gtatcccctt catggcgagc
    12841 gtattcgttg atgatttcat tcttgcgttc ttttgaaatt gccatttctt tcacctcttt
    12901 cgattattcc cctgcgactg agtaaaacgt tggtgattcg cttaaccaag tggcaggtca
    12961 tgtgcctaga gcacacagct atactttacc tgaaaccggg ttgaaaatca agtagttaat
    13021 cattaagaaa aggtagtttt caaacaatct tactactact tttccatttt tgtggtaaac
    13081 tataatgcga aaatagactt gaaacacagt cttttaagaa aggggtacat attccatgat
    13141 tacaatggat gatatcattc gtgaaggaaa tccgacctta cgcgaagttg caaaagaagt
    13201 ttctcttcca ttaagcgaag aagatatctc tttaggaaaa gagatgcttg agttcttaaa
    13261 aaacagccaa gatccaatca aagctgaaga actacactta cgaggcggtg ttggcttagc
    13321 tgctcctcaa ttagatattt ctaaaagaat cattgctgtt cacgtaccaa gtcctgaccc
    13381 agaggcagat ggaccttctc taagtactgt tatgtataat cctaaaattt tgagccattc
    13441 tgttcaagat gcttgtttag gggaaggtga aggctgttta tctgttgatc gagaagtacc
    13501 aggatatgtt gttcgccatg ccaaaatcac tgtgtcttat tatgatatga atggtgaaaa
    13561 acacaagatt cgtttgaaga attatgaatc gatcgtcgtt caacatgaaa tcgaccatat
    13621 caacggtgtg atgttctatg accacatcaa cgatcaaaat ccatttgcac taaaagaagg
    13681 cgtattggtg atcgagtaga gaattggcag gttccttcga tactatgcta caagaaagct
    13741 ttataaaaaa ggggagtgct caaaagctgc acttcccttt tttatgtttc tccactaatc
    13801 gaaatttaga tagacatcta gaaatcgcaa ttccaaaaaa ttctgtgtat tggatacgca
    13861 tcttttctta ttttttctta tttcttccat atgggtgttt atctcagaga tgatagaata
    13921 gtttctagga taaaaagtag cagctacgat gttatccttc gtagaggatt cctgcttcag
    13981 ctagtttttg tttgatgttt ttcagcatat gcttttctgg gtagcggatc gtatgcagat
    14041 ggatgccgcc tgttaattcg gataacaaag tagcattact tttttgataa cgtctcataa
    14101 aggaatcgat atcttgtggc gttttgatgt gcaaagttcc tgctaactca ccgtaaaggg
    14161 gatgttccac aattacatca atgatttcac caccattttc aacgattagt ctcagttctt
    14221 cttctgtttg atcttttcca tgctggacag cgattttact gatgtgccca gtattggttt
    14281 cttctgtttc gaggatataa cctcttgctg ttgccacgat acttttacct gcagctcgta
    14341 gtaaagcaat atcgcctaca atgatctgac ggctgactcc aaaagttttc gcaaacttgc
    14401 tagcactcac tggtttttct gtatttgcca aggtttgcat gatttgtttt cgccgtgtct
    14461 ctccttcgat cacacttatt cctccttcat tttgttttgg tattcgcgcg agtgtttcat
    14521 caaatcaaca tacgtgtctg ttattgtttc cttatatccc ttatttttca gcagactaac
    14581 aactttatcc aatacttgct gctctctttt tgcatcataa actgcttggt tttcttcttt
    14641 tttcacttgt cctattttag ttaccgtatc cattcttttt tctaataaag caaccaactc
    14701 tcgatcgatc atatcgatct tatgacgtaa tgtttcaaga gactcttctg ttagttcttc
    14761 ctctactgtc ttgatgatac gagcaggatt tcctgcaatc actacattgt cgggaaaaga
    14821 tttggtcaca accgaacctg cgccgactac tacgttgtcc cctagagtga cacccggcgt
    14881 gacgataact cctccgccta accaaacatt attaccgatt ttaatgggtt ttgcgtactc
    14941 cagaccgctg ttacgtttca ctggatgtag tggatgcgtc gccgtgtaaa gctggacatt
    15001 cggagcgaac atgcagttat caccgatttc aatcgtgctg acatctaaaa atgtacaatt
    15061 gaaatttgca taaaaattct ccccaacaaa tatattatat ccgtaatcaa aattgataac
    15121 tggctccata tagatttttt tccctgtacg gccaaaagtc tctttcaaca attgggatcg
    15181 cagttccgct gattctgctt ggttgatgat ttggctttga cttcttgcaa acatacggtc
    15241 ggcaaccagc tcaggatcat tggcaaaata aagctcgcct gcaatcattt tttctttttc
    15301 ggttttgaat tcagtcataa tctgctccta attcttgtat ttttttgaaa acaaccgctc
    15361 cgtcttcttg aataaacgta taaggctccg ctaaataaac aggcgtttga ttgattacta
    15421 agattttggc tgacggctgg cgaaactgta ccagatcaca aaacggatga acttgaaaac
    15481 tcgttccaac aatgacgatc agctctgctt ggctgacggc ataagccgcc tgctccatga
    15541 ctccatcttg aaacccttca ccatataaca cgatgtctgg gcgtatttgt cctccgcatg
    15601 cttgatgttt gtcgctgtga agatattctt tccagtctac cgtttgttga cattttcgac
    15661 aatgacattg atacagactc ccatgaaaat tgacgagttg cgggctttta gcctttgcgt
    15721 gcaaaccgtc gatgttttgg gaaacaaccc aaacattttt ttctttcgct agatgtgcta
    15781 tttccaaatg aatgatattg ggttttgctt caggatgata aatctttttg acaaattgat
    15841 agaatttatc cggttcattc accattgctt gatgactcag caagtattcc ggctgctcca
    15901 tcccgtggta gattcctgac aaagatcgat agtctggcac tccagaaggt gtggataccc
    15961 ctgcaccggt caaaaatgta atttgctgat aagctaatat ctcttttgct gcttcgtcta
    16021 ctgttaattg tttcatgttt tgtcacctca ctttccatta tagcccatac tcgaacaaat
    16081 ttattgttga aaagtcagaa aaatgggaat aaaaaagtgt tatgggcatt tgagcaattt
    16141 atcacaatgt tcactgacaa aagaatgaaa acgtgttaca atactgctgt ataaaagtga
    16201 ggtgaatcga tatgttacca ttctttattt attggggcgt tattctggct tgtatcgcct
    16261 ggttagcgtt aagcgtttat ttctctgttt tttatcttgt acgcaaagaa aatggaaatc
    16321 tttgggcttt tgctttattc aacgtgattg ccgcaattgt tttagccatt acattagccg
    16381 tatatcgaac atggggctgg ggaatcaccc aatactcaag tttgatttat ctaatattag
    16441 ccatttatgg cgtcgttgtt cttttgcaag cgatcctagg acgtgagcca aagaaagcgg
    16501 cagcttaaat gatcgagatg gaactggaca caggaaattt tctgttctag ttcttttttt
    16561 attgtattac atacattttt gtgtataatt atactatcgt ttgcaatgga tagatgattc
    16621 acattttgat tgttttttcg gtaaaagtaa agatttctat atttatcagt attttttcaa
    16681 aaaccagcag ttatagattg acaaagcctc tcgagtatcg taaacttttt ttgtataaat
    16741 aactgaatac gagatgcctc aatcaatgag gtagaggtcg cgagaatcat taaccctgtg
    16801 gagtgaagca aacatggaga agcaggaata gggaggatcg ccgaaatgtg cacttttttg
    16861 cttggagtgt atgttgggac gcaagttaag agcttgcgga ctgtcttagc agtgatgcta
    16921 agttgcgcta tgttttgtga gaggtttatc accttatgga attttagcgg cctttttata
    16981 tctataaatc ggtcgctttt tattttttca gataaagctt taccgcattt catctagacc
    17041 gctcgtgtat catataatca gaaagggaga aaaaaatgaa aaaaaatatg gtttcagttg
    17101 ccgtactatt tgcttttgtc ttctcactat tcagtgtggg atctctggca aatgcagatg
    17161 aaaaaacacc gagcgatgag tttcgcgtag gtatggaggc tgggtatgca cccttcaact
    17221 ggtctcaaca aacagatgag aataatgcct tgccaattca aggccaaaat agttacgccg
    17281 gcggttatga tgttcaaatc gctaaaaaag tagcagatgg tttagggaaa aaattagtca
    17341 tcgtccaaac gaaatgggat ggtctggctc ctgcccttca atcaggcaag atcgacgcga
    17401 tcatcgccgg aatgagccct actgctgaac gacgaaaaga aattgatttt accgatcctt
    17461 attacgaatc acaattagta gtagtagttc aaaaagatag caaatatgca aaagcaaaaa
    17521 gtttagcaga tctttctgga gcaaagatca ctgctcagtt aaatacattc cactatactg
    17581 tgatcgatca gataccagat gtgcagaaac aacaagcaat ggataatttc tcagcaatga
    17641 gaacagcttt agcttctgga atgatcgatg gttatgtcag cgaacgtccg gaaggtgtaa
    17701 cggcaactag tgtgaacaaa gacttggaaa tgcttgaatt cagcggtgca gatggcttcc
    17761 agacaaaccc ggaagatgtt cagatttctg ttggtatgcg taaaggcgac ccggatatcc
    17821 aaaaagtaaa ccagattctt tctggcatct ctaacgatga acgaaccaaa attatggacc
    17881 aagcagtcaa agatcagcct gctgcaactg atagtgaaga aaaacctggc atcatttctg
    17941 atttcactac gatttggaaa caatacggca atatgttctt acgaggtgct ggacttacct
    18001 tgttcatcgc attgatcggg acagttgtcg gtactacact tggattactg attggtgtct
    18061 tccgttcggt acctgaatca ggaaatgcac tgactcgctt cttccaaaaa atcggaaatg
    18121 cacttctttc tatctatgtc gaagtattcc gcggaacacc gatgatggta caagcaatgg
    18181 tcatcttcta cggtctggca ctcgcttttg gcatttcatt gaaccgaacg attgccgctt
    18241 tgtttattgt ctctctcaat actggagctt acatgacaga gatcgttcgt ggggggattt
    18301 ttgcagtaga tgaagggcag ttcgaagctg cgcaagccat tggtatgacc catggccaaa
    18361 cgatgagaaa agttgttatt ccacaagttt tacggaatat cttgccagca acaggaaatg
    18421 agtttgttat caatatcaaa gatacagcag ttttaagtgt aatcggcgta gctgatttgt
    18481 tcttccaagg aaactctgct tccggtgcga atttccaatt cttccaaaca tttacgatcg
    18541 tcgggatcat ctacttagtc atgaccttta cgatcaccag aatcctgcgg gcggtagaaa
    18601 agaaaatcga tggtccttca gcttatacca agcttgaaga aatcgacaat tcaaatttgc
    18661 aggaataagg aggaagaatc atgaatgaaa aaattattga aatcaaacat ctaagaaaaa
    18721 gcttcggtga aaatgaagta ttgaaagata tcagcgtaac agtcaataaa ggtgaagttg
    18781 tgacgatcat cggttcttcc ggttctggta aatctacttt cttgcgctgt atcaatttac
    18841 tggaaaaacc aacaggcggc gagatcattt ataatggaga aaatgtatta gctcctaaat
    18901 ataatttacc taaatatcga acgaatctag gtatggtttt ccaatccttc aacctgttca
    18961 acaatatgaa tgtcttggaa aactgtatgt ctggtcaaac aactgtcctg aaacgagata
    19021 aagaaactgc caaaaaagta gcaatcgaaa atctggaaaa agttggtatg gatcgttata
    19081 tcgaagccaa accagcacaa ttatctggtg gacagaaaca gcgtgtcgca attgctcggg
    19141 cattatcaat gaatccagat gtattgctat tcgacgaacc tacttcggcg ttagaccctg
    19201 aaatggttgg cgaagtgctt aatacgatca aagacttagc acatactggc ttgacaatgc
    19261 ttatcgtgac gcatgagatg gaatttgcaa aagatgtatc tgaccgtgtc atttttatgg
    19321 ataaaggtgt gattgcagaa gaaggtacac cagaagatat ctttgtccat ccgaaagaag
    19381 aacgtacaaa agaattcctt tcacgtattt taaatgctta ggatcaatag ctgattggga
    19441 caaagagctg cggtgtttac cgcagttctt ttttatgaat tactcataaa aggtaaattt
    19501 ctgaaaattt tggaatctta gttgtgtttt tcagaaaact ctcttaatat ggaagtaatt
    19561 aaactttttt agttaggatg atttaaaaat gaatgaagag caagaactgc agcgtggact
    19621 gaaaaataga catgtccaac tgatttctat tggcggagcg attggaacgg ggttgttttt
    19681 aggttccgga aagtctatcc agctagctgg cccttcgata ttgctcgcct atcttattac
    19741 tggatgcatc tgttttttca ttatgcgtgc gttaggcgaa cttctgttat caaacaccaa
    19801 taaccattct tttctggatt ttgttgcaga atatcttggc aaaaaagcgg ctttcatcac
    19861 tggctggacg tattggttct gctgggtatc gattgcaatg gccgatttaa cagccatcgg
    19921 aatgtatgta cgctactggg cgcctggtgt tcctcagtgg cttcctgaac tgattgcatt
    19981 aggtcttctt ctttgcttga atatggtggc tgtctcattg tttggtgagt tagagttttg
    20041 gtttgcattg atcaaagttg tagcgatcat tgcctttatc ctcgtaggag cttatatgat
    20101 cctcacccac tatacgacag atattggcac agccagtatc acgaatctat ggtctcatgg
    20161 cggattcttt ccaactggtg caaaaggatt tatcttagct ttccaaatgg taacctttgc
    20221 ttttgctggg atagaattag ttggattagt cgctggagaa acagaaaatc ctgaaaaagt
    20281 gttgccggaa gctatcaata atattcctat caggatcatc ttattttact taggatctct
    20341 ttttgtcatt atggcaatct atccatggaa cagcttgaat ccagataaca gtccatttgt
    20401 agaagtattt tcggaaattg ggatcacgat tgctgcttcc ttgatcaatt tagtcgtatt
    20461 aagtgcagca gcttctgcct gcaacagtgc aatatatagt acaggacgta tgcttcgttc
    20521 tttagcgcaa gaaggcagtg cccctaaaaa attcaaaaag ttaacaacac atcatgtgcc
    20581 agggaatgct ttgacatttt ctactatcgt cattttcatt tctgttatct tgaattacgt
    20641 gatgcctagt gaagtattca cactagtaag cagtattgcc actacttgtt tcttgtttat
    20701 ctggagtatg cttgtctaca cgcacatgaa atatcgtaaa agtatcctcg gcaaaaaagc
    20761 tcattcattt aaaatgccgt tgtacccatt tagtaattat ctggtatttc tttacatggc
    20821 ctttgtgtgt gtcgtcttgt tcctaggaaa agatacgaga atcgctttat tactaacacc
    20881 tgtgtggttc ttgcttctat tactaatcta tcatatgaaa tatgaacgta agaagtaaga
    20941 aagtagttgt gacagaagtg gtcagcttcg agaaataaga aggaatttac gaaaattgct
    21001 tcttaaattt ttgtgaaatt tcggcttatt tcttggagat aaaagcttct gtcccagcct
    21061 cttttctatt tccccggaaa caaccgctga taataggcat ctgctttttt ataggaaaaa
    21121 atccaaatac cagatacaaa aaaacgtccg aataacccga tcaacggtac aaaaaagaca
    21181 ccggcaatca agcataagtt aatcatcatg gagataagac ttcgacggtc ctttgacaaa
    21241 actctcacga catgggaatc tggtttattt gctttcatga tttgcctcat caagatacca
    21301 aaagaaaaac tccacatcgt gtatactaag acataagcca tctcaggcca aaaagcttca
    21361 gggttacgcc cgacccaact agtaacagaa ggacataaag tcagccaaaa gagcaaaaag
    21421 atattcgacc agaccactcg gtaattaatt cgttctgcca tctgaaatat acgatgatgg
    21481 ttcacccaga tcaccgcaac aaatataaag ctgatggtat aagctaataa tgtatttcgg
    21541 atgacccata aagaatgaaa gctagatgta ttagggatct tgatctctaa tacaataact
    21601 ctcaaaacaa tggctaccac tccatcagta aatgcttcta atctcgactt tgtcacgatg
    21661 attcctcctc agatgattca gcgcttttat tttatcactt acgttattaa ataaccatcg
    21721 ttttatatag ggaattttct gaaaattgtt gcatattttt cgaaattttg ttacttttaa
    21781 aaaaaccgta aaggagttat gtatgacaaa aaaattatca tttaaagact atctttttat
    21841 aggctccatg cttttcggtc tattttttgg tgctggaaat cttatctttc ccgttcattt
    21901 gggtcaagaa gcaggcgctg ctaccttttg ggcgaatcta ggtttcttgg ttaccggtat
    21961 cggtcttccc ttcttagggg tgattgccat tggtgtatcg aaaagctcag gtgtatatga
    22021 attagcaaaa cggataaatc aaacatatgc gatggttttt accatcttgc tttacttagt
    22081 tatcggaccg ttttttgctt tacctcgcct tgcaaccaca tctttcgaga ttggtttagc
    22141 accatttatc gatcctagca gacagacgct atatctagca attttcagta ttgtattttt
    22201 tgtgctggct tggtggtttt caagaaaacc gacaaagata ttagattatg ttggaaagtt
    22261 cctgaatcct gcatttttgg ttttattagg tgctttgctt ctccttgctt ttttgaatcc
    22321 actaggttct gtaaatcaag cgcctattca gccaaattat caagagcatg cttttttcac
    22381 agggtttact caaggatata acacattaga tgcgcttgct gctttagcat ttgggatcat
    22441 cattgtcaca acaatccaaa acatgggtgt aacaaaacct gctgaaatcg ctaaagacac
    22501 gatcaaatct ggtgcgatca gcattatatt gatgggaatc atttatactt tgctcgctta
    22561 tttaggtgct atgagcttag gtagctttgc tctaagtgaa aatggcggga tcacgctggc
    22621 tcagattgct gatcattatt taggtactta tggcagtatc ttattagcat ttatcgttat
    22681 attagcttgc ttgaagaccg gtattggttt gattacagct tttgcagaaa cattcacaga
    22741 tttgtttccg caaagaaatt acgcattctt tgttgcactt gctagtatcc ttccttgttt
    22801 agcggcaaac gtaggcttga cgaatatcat tcaattttcg ttgcctgttt tgatgttcat
    22861 ctatccatta gcaatgacac tgattttact agtgttagtt ggtcctttgt ttaagcagcg
    22921 tacggctgtt tatcgaatga ctacttattt tacattgatt gcttcgattt ttgatgggtt
    22981 gaacgcttgc ccagaaacta tcaaacaaac acctattgtt caaaatcttt tatatgttgc
    23041 ggaaagctac ttgccgtttt tcaaattggg gatggggtgg atcgtaccag cagttatcgg
    23101 ttttgttatc ggactgattt ggagtttcgc taagaaagaa gaagtagcag attgataaaa
    23161 gaggttgtga aagagacgtt cagccttgag caataaaaag taattttgaa aaatggcttc
    23221 tcacattttt gcataaattt gacttaatac cgagaaactg acttttgaac accgtttatt
    23281 gagaggtcgt gaaaaagccg cttagtttca agtattaaga aatattttgt gaaaatggct
    23341 tctcacattt ttgcataaat ttgacttaat accgagaaac tggcttttga acaccgttta
    23401 ttgagcgtct ataaactgac ctgagtatta gagaaacaaa acggcaaagt agctccatat
    23461 actttgccgt tttgttttga actaataccg aagagaagct gatcaaacaa catttctttt
    23521 ataatataga ttttagacat atatatgata taaaaatagt tttcgcaaa
//
LOCUS       DAMEET010000044        22301 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.045, whole genome shotgun sequence.
ACCESSION   DAMEET010000044 DAMEET010000000
VERSION     DAMEET010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.045"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(48..1343)
                     /gene="purB"
                     /locus_tag="PB780_001509"
     CDS             complement(48..1343)
                     /gene="purB"
                     /locus_tag="PB780_001509"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDE0733566.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1356..2480)
                     /gene="purK"
                     /locus_tag="PB780_001510"
     CDS             complement(1356..2480)
                     /gene="purK"
                     /locus_tag="PB780_001510"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDE0733567.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2473..2961)
                     /gene="purE"
                     /locus_tag="PB780_001511"
     CDS             complement(2473..2961)
                     /gene="purE"
                     /locus_tag="PB780_001511"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDE0733568.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3377..4684)
                     /locus_tag="PB780_001512"
     CDS             complement(3377..4684)
                     /locus_tag="PB780_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDE0733569.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4687..5268)
                     /locus_tag="PB780_001513"
     CDS             complement(4687..5268)
                     /locus_tag="PB780_001513"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDE0733570.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5384..5481)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5840..7132)
                     /locus_tag="PB780_001514"
     CDS             complement(5840..7132)
                     /locus_tag="PB780_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDE0733571.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7129..7752)
                     /locus_tag="PB780_001515"
     CDS             complement(7129..7752)
                     /locus_tag="PB780_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733572.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7755..8591)
                     /locus_tag="PB780_001516"
     CDS             complement(7755..8591)
                     /locus_tag="PB780_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDE0733573.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8608..9495)
                     /locus_tag="PB780_001517"
     CDS             complement(8608..9495)
                     /locus_tag="PB780_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDE0733574.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9511..10794)
                     /locus_tag="PB780_001518"
     CDS             complement(9511..10794)
                     /locus_tag="PB780_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDE0733575.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11477..12160)
                     /locus_tag="PB780_001519"
     CDS             complement(11477..12160)
                     /locus_tag="PB780_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDE0733576.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12178..12795)
                     /locus_tag="PB780_001520"
     CDS             complement(12178..12795)
                     /locus_tag="PB780_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDE0733577.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12805..13629)
                     /locus_tag="PB780_001521"
     CDS             complement(12805..13629)
                     /locus_tag="PB780_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDE0733578.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13622..14182)
                     /locus_tag="PB780_001522"
     CDS             complement(13622..14182)
                     /locus_tag="PB780_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733579.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14356..15312
                     /locus_tag="PB780_001523"
     CDS             14356..15312
                     /locus_tag="PB780_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDE0733580.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMIHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15617..16573)
                     /locus_tag="PB780_001524"
     CDS             complement(15617..16573)
                     /locus_tag="PB780_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDE0733581.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCERLLTFIRESQEDAREHQENYWLRQPLYYETCFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILARIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16678..17280
                     /locus_tag="PB780_001525"
     CDS             16678..17280
                     /locus_tag="PB780_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDE0733582.1"
                     /translation="MQFGRFRLGMRTIKSAIAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17368..18867)
                     /locus_tag="PB780_001526"
     CDS             complement(17368..18867)
                     /locus_tag="PB780_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HDE0733583.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMNEE"
     gene            complement(18883..19407)
                     /locus_tag="PB780_001527"
     CDS             complement(18883..19407)
                     /locus_tag="PB780_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDE0733584.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19582..21231
                     /locus_tag="PB780_001528"
     CDS             19582..21231
                     /locus_tag="PB780_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HDE0733585.1"
                     /translation="MFPFFKKKPLKLQNTIILVVCFSTFVSLIVSAILIRNFILSMNR
                     SSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIMIASNLDFIVVMDPEGIR
                     YSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFRYFVPIYYNQEIIGAVCV
                     GLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMKKVLLGLEPQEIAEQLSE
                     KTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDFEVGKKISDNLYDVLFKE
                     TIEHQTTVRTQSMYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGTKQ
                     YINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVPAI
                     AGFLLGKINEAKEQSVEMLLETDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQKHE
                     RNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLIHS
                     IVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21200..21916
                     /locus_tag="PB780_001529"
     CDS             21200..21916
                     /locus_tag="PB780_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDE0733586.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     LKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22018..>22301)
                     /locus_tag="PB780_001530"
     CDS             complement(22018..>22301)
                     /locus_tag="PB780_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HDE0733587.1"
                     /translation="LKSNHLAGFATDVFANETEVFFRSFKPWETIPDPAIQQLVEMYP
                     RVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPKARQLR"
BASE COUNT         6567 a         4806 c         3904 g         7024 t
ORIGIN      
        1 ttatttttgc tattctattt taattgaatt ttgcaactca ctcttcttta ttccaaacct
       61 actcgtttga atatcacgtc aacgtttttc aaatgatgat gataatcaaa ggcatcgtct
      121 atttcttctt tagataatac cgatgtgatg gttggatctt cttctaacag cgggcggaac
      181 tgcacttggt tgtcccaagc gtacgcagtt tttggctgaa caagatcata agcttcttct
      241 cttgtcattc ccttatcgat caatttcagc ataacgcgct ggctataaat caaaccaaat
      301 gttgcagaca tattccgctt catgttttca ggaaagaccg ttagattttt aacgatattg
      361 ccaaaacgat ttaacatgta attcaataaa atcgtggtat caggcaagat gattcgttct
      421 gctgaagaat gagaaatatc tctttcatgc cataatgtga cattttcata ggcagtgacc
      481 atgtgtccgc gcaccactct ggctagacca gccatatttt ctgaaccgat cggattcctt
      541 ttgtgcggca tagcagaaga acctttttga cctttcgcaa aaaattcttc tacttcacgt
      601 gtttcggatt tttgcaatcc gcgtatctct gttgcaaaac gttcaatact tgtagcaata
      661 agcgccatcg ctgagaaata ttcagcatgt agatcccgag ggagaacttg agtagagatt
      721 tcttgtggac ggatccccaa ttgtttacat acatactctt caataaatgg cgggatattt
      781 gcgaacgttc ccactgctcc tgagattttt cctgcttcta ctccttttgc cgcatgttca
      841 aatcgttcga tgttccgttt catttctgaa taccatgtgg caagtttcaa tccaaaagta
      901 gttggttcag catggacacc gtgtgtccga cccatcatca ctgtatattt gtgttctttt
      961 gctttttcgc caatgatttc cgtaaaacgt ttcaagtctt cccgtaaaat atcatttgct
     1021 tgcttgatga gatagccata agcagtatct acgacatccg tacttgttag accataatgg
     1081 acccattttc tttcttctcc caatgtttca gaaaccgctc gtgtaaacgc aacgacatca
     1141 tggcgtgtta atttttcgat ttccaagatc cgatcgatat caaatgatgc atgctcacga
     1201 atttttttca catcttcttg cgggatttca ccaagttctg cccaagcttc atctgctaaa
     1261 atctcgactt cgagccacgc acggtatttg ttctcttccg tccagatatt ccccatttca
     1321 gggcgtgtat aacgttctaa catgtttttt ctcctctaat cccaaatatt cgtttgtttg
     1381 atttcttcca gcgtttgctg gatgtcttct gtcaagatag tgatatgtcc catcttgcga
     1441 cctttttttg cttgtttttt tccatagaaa tggaattgcc agtctggctt attctcaatc
     1501 aagcgatagc tttccagcag ttgttctccc aaaacattga ccattactgc attgcttatc
     1561 aaacgtactt tttctcctaa tggccaacca caaatacctc gaatatgggc atcgaactga
     1621 ctcatagaac aagcttcaat agaataatgt ccagaattgt gaggacgagg agctaattca
     1681 ttcacatata gcccgcctgt ttttgttaaa aacatttcta cccctaaaac acctgaaagg
     1741 ctcactgctt ctgcaatcac acgggcaata cgctcagcct cctcgatcac atcctgatca
     1801 atcgcggctg gagcaatcgt ttcatgaaga atattgttgt ggtggatgtt ttccacgacc
     1861 ggaaaagtcg tgaaatctcc ttgaccattt cctgccacca tgatcgaaat ctctttttca
     1921 aacgggatcc atgcttctaa aacacaggtt ccttctctta aaaggtccat ggcaggagct
     1981 agatccgccc gacttttcaa aacatactgc cccttgccat catagccgcc tcgagtggtt
     2041 ttcaaaacac aaggatagcc gatcccgtca atcgcatctt ggatatccgt cggactgacg
     2101 atcgttgcat aaggagcaat cacgatgtta ttcgtttcca aaaaagattt ttctaacagg
     2161 cggtcttgcg taatcgcaag caagtctgtc ccttgaggaa taaatgacat gggtaaaatc
     2221 gcatttaacg cctctacact gacgttttcg aattcatagg taatgacatc tgttcttctg
     2281 gcaagctctt tcagcgcaaa ggtgtcatca tagacacttt caatgtgcca atctgcaact
     2341 tgtgcggctg gacaatcgct cacaggatcc agcacaccta ctttaaagcc catttctttt
     2401 gcactaatcg ccatcatacg acctaattga ccgccaccga cgatcccgat cgttgaacca
     2461 ggcaacaaag gtttaaccaa gttgatcact actttctata acagttttag ccaacattga
     2521 acgtctttca gccaatttag cctccaattc taaatcatac atggaaagca tctgtatcgc
     2581 aagtagtcca gcattaattg ctcccgcttt tccgattgct gtagtagcta caggtactcc
     2641 tccaggcatt tggacaatcg aaagaagcga atccaaacca tttaatgttc gtgattggac
     2701 cggtacaccg ataaccggca aagttgtttt tgatgcgacc atacccggca gatgcgcggc
     2761 tccccctgct cctgcaataa tgattttgat tccttttttt cgagcgtttt gtgcatattc
     2821 aaacatcaaa tctggggttc gatgagcaga aacgaccttt ttttcataag acacaccgaa
     2881 ttcatccaat atttgacatg cttctttcat tgtttcccaa tctgaagtac tccccataat
     2941 cacagagatt tttgctgtca ttgtttttcc tcccagtttt tctacattca tattttaaca
     3001 atcacttttt ttgatgtcaa agaaatttcg aatgttaaca aagaaaaaca ttttattgtt
     3061 cgtgtttatt aaagataaaa aaaaggaatg tgacaaaagt taggtcacat tcctttttat
     3121 ggataaacgg tgttctcctg ccaactcctc ggtcacaagg aacaatattc aacaaacatg
     3181 agaagctttt ttttgaaatt gttccttgtg actcggagat gaacggcagg ctcacgacct
     3241 cttccttcaa cggtgttcaa tcagctgacc ctcggtatta gctcaaacaa acggcaaaat
     3301 acgaggaact atttttccgt ttgtttctct aacaatcagg tcaaaacgct gatttcacaa
     3361 cctttctttt gctctattac ttcttgaagg cttcttccaa tatttcttca tcttttttca
     3421 atccattaaa gatcaaattc aatatgatag ccgtaatgct actcattacg atgccatttc
     3481 cagtgaacat ttgcaatgtt tgcggcattt gactgaataa tgtcggcgta acattgaaac
     3541 ccagcccaaa tccaacagaa acagcaacaa tcaatagatt tttatcattg ctgaaatcaa
     3601 cttccaacag catacggatt ccttggactg ccaccatccc gaacatgact aacattcctc
     3661 caccaagtac cggctcaggg atgatttgtg ctaacgcacc gattttaggg aataatccta
     3721 aaacaatcaa gaagaatgca gagaaataga tgggttttct tgttttgatt cccgaaagtt
     3781 gaaccaatcc gacattttga gaaaagccag tataagggaa tgtattgaaa ataccgccta
     3841 agataacggc aagtccttct gcacgatatc cctttttcaa ttcttcttct ccaacatttt
     3901 tcccagtaat gtcaccaagt gcaaaatata cacctgttga ttctaccatg ctgaccatgg
     3961 aaatgatgat cattagtaaa atcgaccata cgtcgaaagt tggtttacca aaataaaagg
     4021 gttgtggaac atggaacaac ggcgcatctc caatcgcgct cagatcaatc attcccatga
     4081 aagcagctaa gatgcttcct ccgactaaac caatcaatac cgcgattgaa cgaatgaacc
     4141 cttttgccag catttgcacg cctacaatta ggagaatcgt cacaaatgca agtagcagat
     4201 ttgttttgtc accaaaatca gctgctgttg cattacctcc acccattttc tcgattgcta
     4261 caggaatcaa agtcaaacca ataacggtga taaccgttcc tgttactata ggagggaata
     4321 gttttttaat tttagaaaag acaccagaaa ctaacacaac aaagatccca gaagcgataa
     4381 tagatccgta gatagctcct actccttcat tggttccgat caagatcagt ggagctactg
     4441 cttggatcgc acatcctaaa acgacaggca agccaattcc gaaaaatcgg ttaactgtca
     4501 attgcaataa tgtcgctaat ccacacataa agatatcaat tgaaatcaaa taagtcatct
     4561 gttcttgatt aaagcctagt cctgtaccga tcaaaagagg caccgctact gctcctgcat
     4621 acatcgctag cagatgctgc agccctaaaa ccgcagcttt tccattttgt gtttcttttt
     4681 ccacgattat gcgtcctcct caagaaactc aacttgtcca ttcgtcaagg aagcaatacg
     4741 cgcaagagaa acgactctca atcccatttc ttcaagtaac tgacgtccat cttggaaaga
     4801 cttttcgatg acgatcccga ttccttctac ttgcgctcca gcttgctggc ataattcgat
     4861 caatcctttt gccgcttgtc cgtttgccaa gaaatcgtcg ataatcaata ctttgtcttc
     4921 tgaagataaa aatttacgag aaatagaaat cgtacttgtc acttgtttcg taaaagaata
     4981 aacagaagct gtcaataatt cttcatccat cgtcaaactt tttgcttttc tagcgaagat
     5041 cattggaaca tctaattctc ttgcagcaaa taaagctggt gcgatcccag aagcttcaat
     5101 ggtcacaact ttcgtgattc cttgttcttt aaatacttct gcaaaccgtt ttcccatttg
     5161 ttccatcaat acagggtcta cttggtgagt gacgaagctg tcgactttta atacgccctc
     5221 gcctaatata cgtccgtctt tttgaatacg ttcaactaat tctttcactt tatttgctcc
     5281 tttatcattt attaataccg aaataaccgc ttttgtgcaa aaacctacta tgtttctgtt
     5341 aaagaaaaac accctccact ttatcataag aaaagaaggg tgaaatagcc aatcttttac
     5401 ttatagtcca gcatttacgg tactggctag aaactgtcat ccatattatg acgatatata
     5461 agtaggtatg taaaactaat aatagtgtaa attcattttc ttgcaactgt tttgcacgtg
     5521 aaggcagttt gccatatttg aataaaaaat gttgtttcaa atattgaaaa cagtctaaca
     5581 ctactttctg aaactgacaa cgtggtttac ttattttttt gtaaaatcta atgattttta
     5641 atgcattatt cctttttgtt cgggtttaaa tagttatttt tattttctcc tatctaaaaa
     5701 agcagcccac ataaaaagaa ggcatgacaa aaagcatgtc atagcccctt tttccgaata
     5761 aatagtgttc aaaagacagc ctatcggcaa taaggcaaga ttttataaac tacgggaagt
     5821 aatttccgta aatctattct tattctttta agctgacaag ctcgtcaatc actactgctt
     5881 ttcctgaagc aatagaatga tttgctgcga ttcctgtcaa aatcgacaaa ataccatcta
     5941 cgtgagttgc tgcccgattc cacggatctt cttctttttc ttcaccaaat agatcatcta
     6001 ataaacgcgg atctcctccg ccatgtccgc ccgcttcttg ttcgacgatt acctcatatg
     6061 gctcaccgaa catcggatag acatggatgg actgaagaac ggctgctcct tcatcctttt
     6121 tatctccacc tgcattgatg taagactttt cgatcacttt catttcgatc cgtcctttgc
     6181 tgccattgaa agaaacattg aatccttccc acggcatata cgcgtttagc gaatacgtca
     6241 aaatcgcacc agtttcatat ttgaccgtta cgcctagtgt atcttcaata gagatatcat
     6301 cggaaaatac actgcggtca cgaatatatc cactgtcttt ttctgcttct aaatacattt
     6361 ctcgcaattc ctcattggct gacaggtcta tagcaaatgg atcagcttta gctagttcgc
     6421 ttccatagca tcgactgtaa aatatttcta ctccgcgttt ctccgcatta tgcatcccat
     6481 aaaagtttaa tccaccgaat gcaaatacct tttggggacg gctgttcaac caaaaattca
     6541 ctagatcaaa atgatgggta gatttatgaa caagaagccc gccgctattg tgcttatttc
     6601 gatgccatct tctgaaataa tctgccccgt gttgcgtatt taacagccat tcgaagtgga
     6661 tagaaaacac atcaccaatc acatcacttt ctatcagttc ctttatcttc gtgtgatacg
     6721 gtgcataacg ataattaaaa gtgactcgca ggcttcttcc cgtctgtttt tggaccgtta
     6781 ttatttcttg agctttttcc tcatcgatag tcagaggttt ttcgcaaata acatcatatc
     6841 cttttttcat tgcacgaatg atataatcat gatgcgttcg atctactgac gtaataatga
     6901 cttcagccgg ccgtacgtca tccagtagtt gctcaaattg tgatggcata catgtcttta
     6961 cttttttctg tccatacttg ttcacaagta tccgatttgc ataatccatc cttgtttgag
     7021 atacatcaca aaaaccgact agttcacaag tttgatgata cgtagaggca attgcctcat
     7081 aaaaaaaacg cgctctccca ccaagtccga cttgtacata ttttttcttc atttaaagtc
     7141 attctccttt tcttttaagc aaaatactgt gacaaatggc agccctggat acagtaatgc
     7201 agtgagtaaa aaatcatgct gtatacacca gaacagtcca tacaaaaggc ataaatacca
     7261 cagaaacccc ttgacgtttt ttgacaacag ccagatattc cttttgatca aataaacaag
     7321 actatctgct ttatcttctt tgcagatatc aattagcgta ctaaaaaaac tatgggatac
     7381 aaaaagcaac agacacaata gaaaaggaat cagataccaa gctccaatct ttcccactaa
     7441 taaataccaa tcaactgcaa aaataatgac aaacagctga aacaacgtaa tgatgaaaca
     7501 catccgtttg atttttcctt tcctccaaca tacctttttt tctgccaagt aatggacaga
     7561 tagataataa gaaggataca tcatcaaaaa ataaatcaga tagacaacac gagcagacag
     7621 cactaatcct aaagtgaaca aacatcctag aactaaagcg aaaagagcat tactgataag
     7681 actggttaaa aaaatccgac agacatagta gttgagcttg taaagaaaca tctcttctat
     7741 tggctttttc atattcagcc cttcagaccg cttgtactga tcccctggac aagatattta
     7801 ttgaagaata agaaaatcag aaaaaccggt agcagcgaca acgtagacat cgcaaacata
     7861 ctcgcgtaag tcgtactaga tccagcgtct gcaaataatt tgatggcaat cgataccgtg
     7921 taagatctcg gcttgtttaa ataaatcaat ggtcccatga agtcgtccca tttccaataa
     7981 aactggatga tgatcgtcgt gatgatcgaa ggtttcagca aaggaaaaat aattttggta
     8041 aagacactat acttgctgca tccgtcaata atcgctgatt catctaactc tttggggatg
     8101 ctgaccatga attgcatgat ctggtaaatg aaaaatgctt gtccaaaaaa atgtggtaaa
     8161 atcagcggca catacgtccc aaccaaattc agccgattgt aaataataaa ctgcggaatc
     8221 aaaatcactt gtgcaggaat catcatcgtt actaacatga cgacgaacca gaatcttttt
     8281 cctttgaaat cgattctcga taatgcgtaa gcaatacaag tcgagctcag aactgtccct
     8341 atcgtcgaaa ctgcagagat gacaaacgaa ttcctaaaga aagtaaaaaa tgaaattcca
     8401 gcaaaccccg cccatccttt tgcgtaattt tcccattgaa aagtctctgg aatcaaattc
     8461 agcgaaccac tcataatctc ttgatccgtt ttaaaagaac ctgcgatcat ccataagacc
     8521 ggataaatca tgactagacc taatagcaaa ataaaaccgt attgaaacca tttcgttact
     8581 gtattattca tttcttcctc ctctcattca cacaacgacc catttttttg atgatttgaa
     8641 aatcagaaag gtgacagctc ccataatcaa cagcatgatc cacgaaatag cactagcata
     8701 ccccatagaa ttaaaagaaa aagcttggtt atacacataa agagcataaa agttcgtggc
     8761 atcattcgga ccaccttggg taatgataaa tgcctgcgta aaagtcataa acgccgcgat
     8821 tgtctgcata accaagttat acaaaatgat cggcgaaaga agaggtaacg tgatcttgta
     8881 aaacgtctgc catttgttcg ctccatcgat ctctgccgct tcataatacg agcctgggat
     8941 ctctttgata cctgcagcaa agatgatcat tgccgaaccg aattgccaag cagacatcaa
     9001 aacaagaggg atcatgacca gtttttgatc gctaaaccag gaaagcgcag gtgcgcctaa
     9061 tcgttccagg aaaatattca ctaacccgtt tttagaaaac aactcccgcc aaactagagc
     9121 gatagaaata ctgccaccga tcaaactagg cacataatac aaagaacgaa agaaagtcgt
     9181 tcctttgata ttcctcgtta aaagataagc aatcaataga ccaaagatca gtttcacagg
     9241 tacagatgca aatacataga gaaaagtgac tcggatggag ttcaaaaaac gggcatcact
     9301 cagcatcttt atataattat ccaagccgat ccatgagact ggatttccca aacgataatc
     9361 ggtgaacgaa taataaagtg acaagatcat cggtatcact gttatcaata gaaatcctag
     9421 gatgaatgga gaagcaaata cgtaaccgac cacattgtct tgatccaaga accgttttag
     9481 tttttctttt ttcatgcctt cctcctgctg ctatcctaga atgtttgacg caaactcata
     9541 aagactctct gctgcttctt ctgctgttac ttcttcataa atgacttttt caactaaaag
     9601 attgtattgg tcttgaattt ccggattcaa agcactatca aagacattca cttcgccgtt
     9661 gtccatatcg gaaatcattt ctaaataatc aaatgtcgct tgcaaagttg catcatctgc
     9721 acgagcgagc tgttctcgaa tattcgacat gattggtaca ccacgttctc cttttaaaat
     9781 ttcattggct tcttcactgt tttgaaagaa atcaataaac ttagcagctt cttctttatg
     9841 ggctgagctt tttgccatac tcagcatttg cgaagaacgt aaagggatac cagattctcc
     9901 atctgctttt ctgcgaggtg gattaatcaa ctgaatctcc atatcttcag gagcccctga
     9961 caatatttct gtcaactggt tacttgatag aaacaccatt gccgcttctc tatccctaac
    10021 cgcctgaaaa gacagatccg tcgtacttgc agctccacca cctggttctg gatgggcccc
    10081 tttgtctacc agatttttac gcagctggaa aaaatctgtt agatagtctg gattttcaaa
    10141 tcccaagctg ttagatagat cttcccgatt ataaaaattc aatcctttct caacttgtgg
    10201 tatttgaata atacagccag ctataaaatc ttcgaatctt gaaatagcat agattcctaa
    10261 ttcttcagtg atttgatagc tgatttgttc aaattcatcc catgtccaat ttgaagctgg
    10321 ttcagggata tttaattgtt caaatatatt tttattgtaa gcaatcgcaa acgaattcac
    10381 cccgttagac agtccaacta attgcccgtc gtaagtcgtc gttgctaaat aggattcatc
    10441 tgcattagaa atatcaatta tcccttgctc cacatattca tctaatggtt caatctgatt
    10501 gatgtattga ggaaattgat tgttcagttg gaaaatgtcc cagatatcat ctgatgctgc
    10561 tagtgtacct aattttgtgt aatatccttc ataatcataa aattcataat cgatcgtcac
    10621 atggggattt ttttcttcat aaagctcgat tacttttact gttgcatcgt gtcggacttg
    10681 cgaaccccac caagccatgt tcagcgtgat ttttttgtca tcttcttgcc cacaagccgt
    10741 caagaagaat aacataacac ttaaagctaa gatgattctc attcttttct tcattctctg
    10801 cacctctact tccattgtta tttttcaata tactgtattt ttatacagaa catatttatt
    10861 aaacgatctt tttcaagggg aataaaaacc aagacttgcc ttatcgttct gcctaggatg
    10921 aacaactcaa tactttcaag aaaaattaga tagtgctgat tcttctttca tcgctaaaaa
    10981 cacttcttgc ttcaatgatg ggaggataat tacgcaaaga aaaaaggata tcagacaact
    11041 gcccaatatc cttgacatag ctaaataagc atgcttcttc gtgtgcgctc ttccctaaaa
    11101 aacagaaaaa taactagtaa ttctgtatga aattaggtac aatatacacg tcgatgcaca
    11161 agcattgact atggcaactt gattggtcag ttcccgctga cttttcaagc cccgacaatt
    11221 tagctgctac taaattgtcg ggtgcctttt ttagttttta ttcgtttttc attctataga
    11281 aagcgcttca ataaatcaag acattttttt gttagaatgc taataataac aaaaggtttt
    11341 aactcttctt atgtacatta caggtaaaaa aacaaaaaaa tttctctttt ttttgaattc
    11401 tgaacaaata gtactggagt acaaaagacg aggaagagcc actatctgct cttcctcgtc
    11461 ttttgattct cacctgttat tcatgattct ttttctcagt ttcccgatat ttttccattt
    11521 tacgactaaa aagctctccg ctacttggtg gcgtatatgt gatggcattt gctcctgctt
    11581 gaatggtctc agcaatactt tcttccgttg gaccacctgt cgcaatgata ggaagttcag
    11641 gatatttttt acgaaaatgg cgtactgctt ctgcagtttc tttacctgca ctgatattga
    11701 taatcgaaat accagctgct aacttttcat ctatttctgt atatttcgac gttaccgtac
    11761 tgataactgg aatatcaact actcgacaaa cttcttttac tgtctctaaa ggcgtaggac
    11821 cgttcaaaac gacccctaac gaaccttgcg cttctgcgaa catgctcatg taggaagaac
    11881 gagcaccttg tgtcaaacca ccacctacac cggagaaaac tggaatgtca gctgcctcaa
    11941 taatgctttt agtaatcgct ggatgaggtg taaaaggata cacagcaatt actgcattcg
    12001 catttgtatt acgtatgatc gcaatatccg tagtaaagat gatcgaacgg atcaattttc
    12061 caaatatctt gatcccgctt gcttctaaaa tgatttccgg tactcttacg atatcttttc
    12121 tcaattccgt catgatttct ggcgtaaatt gttctgccat cagccacttc tcctttgcta
    12181 tctccggtca tactgattca attgatacgt ctcgatgacc tggatgattt tttgcgcata
    12241 accggggtct gtcgcataac ctgcatcttg caatgcttgt gcggcttcct gatagttggt
    12301 ggccgtaatc actttttcat acagcgcagg attccaatct actccttgga cgaacagttg
    12361 tgtatggtca tccatggact gttcccatga atcatacact ttgaagtctc cttggatagt
    12421 tatccattct tcgttaacaa attcctttgt ttccaggctt actttcttct gatccccata
    12481 agccttgatc ccgaaaagat tattgtattt gctagccaag gtactttttc cccaatttga
    12541 ttctaatata gcttgtccta aaatgatact aggaagcact ccataaccat cttgtagttc
    12601 ttgggcatgc ggaacaagcc gttcaataaa ttgctgatgg gattgcgctt cattttcttg
    12661 ctgattatca gtaaaaggag aagacagatt cttcaaggaa aaaacaaagg ctaagagtat
    12721 cactgctaca ccggcaacca ctgctggcca acggatattc ttgcgctttt gctttttcca
    12781 cttttttttc gccatgatca attcctactt ttcatttaat tcattcagat attcttctag
    12841 cgtcaattgg tgtttggtga tatactcagc gttttcttta ccgacataac gtaagtgcca
    12901 aggttcatat gtgatcttag ttatatcttc gtgtcctttt ggataacgaa cgataaatcc
    12961 atactgcgca gcattatctg cgatccattt agctccaggc tgatctccgt aacttgcatc
    13021 caatacagta gaaggataac tgttgtacca gttttgatcg acgacatcga ctgccagacc
    13081 tgtgtggtgt tcgctgtatc ctggctccgt aatcgtttct ttagtaatct ttgtcgcctc
    13141 ctcttctgat acaccttgac gactcatcac atcttggaca ttttgttcga aaacttgttg
    13201 ttgggatgat acagaccggt aagctgagac catcactaat ggaaatccag ctgcttcagc
    13261 agccttggca aatgcttcat attctggagc aatgcgtttg tcgatcaagt atccgttact
    13321 taatgatgcc aattgagtag actcatcaat ttctttttct aatttgtgat ctggaccaac
    13381 caaaactagc ttccaatcgt ctgttttaac atctggcaat tgtttggacg tacgatcttt
    13441 tgtttctttg tcctttacct tgctgctggt tgtttgactg ttcttttctt tactagcagt
    13501 tgttttggtg cttgttcgtt cgactgcttg attgccggct tgaacatgcg aatcatcatc
    13561 gctaaaaata acatagccca ttgctgcgat cataatcaca actgctccaa atcctaaaaa
    13621 tttattcact tgaaaaatcc tttcgttgtt cttcacgttc cccattcgcc tcatgaacgt
    13681 aagtttcttc cagattttca ttcacccaat cgagaagttc gattttttct ccttcgatct
    13741 gtttttgaac atctgctggc agacgaaaat tcaaaatatc atcatagccc cattcatcat
    13801 attgaatact tactttcaag tcaaggtatc taccagcctg ctgattattt ttcaaaggga
    13861 tcagctcgac ttttgctgct ttgctgctaa gccatttttg cgcgatgcgt gttttgattt
    13921 gcttgtactc tgcaacggca acgtttcgtt tttcgggaaa aataatcgtt tgacgcaaaa
    13981 tcttttgcgt tagcatccat tcataatcag tgaaaagatt ttttactttc tgcatcgctt
    14041 cagatggcag atttttctcg ccatctttcc atttttgcca ttgttctgga ctgatcagca
    14101 agtattcatc taaaaaattt ttttcagttt caaactgttc taataataca ctcataataa
    14161 gatcaatata tacttgatgc acgtttatcc cctcctttta actattctac ttaagttttg
    14221 atcaaaaagc acctcttaaa tcgctgcttt catttttttt aatgtttcaa gtcctctcct
    14281 ctttattctt cgtgattttt gttataataa tagacagaca agaaactcaa tccaactatg
    14341 aaggagcgtt ttcagatgaa aaaatatcaa tggggaatcg ttggtcttgg taccatcgca
    14401 catgagtttg ccgaaagctt caatcaagag acgagcgaac taacagcagt tgcttccaga
    14461 acttcagaaa aagcggaaaa cttcgctcac cgttacaata ttccaaaagc ttacggaagc
    14521 tatcaagaaa tgttagacga tgctgaaatc gacatcgtgt acattgctgt accaaataga
    14581 cagcatatcg accatatctt ggcagcattg gaagcaggca aacacgttct atgtgaaaaa
    14641 gccatcacga tgaataaaaa agaactggct gacgctatgc gcttagcaga agaaaagaat
    14701 ttgatcttag cagaagcgat gacaatcttc aacatgccac tttatcagca gttacgctca
    14761 ataatggata caggaaaact gggggcttta aaaatgatcc aagcaccttt cggcagttac
    14821 aaagaaccag accctaaaaa ccggttcttc aatccagaat tagctggagg agcactgttg
    14881 gatatcggta cgtatgctgt atctttcgct cgtttctttt tgagctctca acctgaagta
    14941 gttgcctcta caatggttcc ttttgaaaca ggcgtggacg aacaatcagt cactatcttg
    15001 cgtaacaaag aaaatgaact tgctgctgtc agcttgactt ttcaggcaaa aatgccaaaa
    15061 gtcggtatcg ttgcctttga gaacggttat gtgacgattg ctgattaccc gcgtgctgat
    15121 cgtgcggaga tcttatttac agatggtaca aaggaattca tcgaaagcgg gaatacatct
    15181 gaagcaatga attacgaaat aaagaatatg atacacatga ttgaaggtga cttgcccaat
    15241 cgctctctct tcttgacaaa agatgtcatc gaaatcttgg atcaaatgca agttctatgg
    15301 aaaaatatgt aattttaaaa ggttgtgaaa aagctgttct gcatcaagta ataagaacag
    15361 tcaaacaaaa atagctcctc ctatttttcg tttggctgga cttattaccg cagatgcaag
    15421 cttttgaaca ccgtttaagg aagaggtcgt gagcctgccg ttcagctccg agtcacaagg
    15481 agcaatttca aaaaacagct tctcatgttt gttgaatatt gtgacttgtg accgaggagt
    15541 tgacaggaga acaccgttta aagaagaggt tgtgaactca gctttttgac ctgaatatta
    15601 gctgaattca caacctctat ttttggattt tttgttggga gaattctgcc ttgatttcaa
    15661 taaaactttt aaaagactgc aaaaactgga acaattctgc tcgatcctcg aattcagggc
    15721 gactagtcgg cagaggcatt tgccgataca atacataaac ttcttcaata cgtgctaaaa
    15781 tttgtctgcc atcatttgac tctgaaaatg tttctgcagt ataaatcaat aaaccataga
    15841 tatgttttcc ataataagct ggttgctgga ttctctctag attttccagc atgtctttaa
    15901 tcacattggc ctgtgctctt ctcatagaga agcaagtttc gtagtatagc ggctgtctga
    15961 gccagtagtt ttcttgatgt tctctggcat cttcttggct ttcgcgaatg aatgtcagta
    16021 aacgttcgca tttgatctgc acatcttctt tattttctga taaaagaaat tctgccattt
    16081 gtctcaaaat atttcgaaat tctttttcga tctgtatttg gttttccttc agctgcttcg
    16141 tacgatctgg catgtaaaca tttgcaagca aagcaaatac taatccaatc gccattaatc
    16201 ccgcttcatt gccgatcagc tgccaagaca tgttttcctc cactaaataa tgagtgacca
    16261 aaaccgaatt tactacgatc ccttcggtta atttgaagcg agaggcaagc gggatgaaca
    16321 ggaataaaaa aagactgaag ccaaaaatag tatacccaac agcaaaaaaa ctgaacagtg
    16381 ctgttgccgt agctaatata aaggctgcga ttcgattcat gcccgtacgc aatgtagagc
    16441 gcttggtatt tcctacactt aataatgcaa tgatccctgc agctggccaa tacaacagcg
    16501 ataagctacc cgctgtgacc atcgaaaaga agacactgat cactgttttg actgttcgca
    16561 ttcctatacg catttacttc cccctttttt tattttaatt atacttgaat aatgaaaaac
    16621 tagctatcag acaaaagttt aggtatgata gtaaaaagac caaaaaggag aatctctatg
    16681 caatttggcc gttttcgact aggtatgcgt acgatcaaaa gtgccatagc tgttttttta
    16741 tgtatcttgt tttttcattt gactcaacga ggaatcccga tgattgctgc attatctgct
    16801 gttttttctt tgcgtcagga cttgactaca accgtttctt ttggaaagtc taggatcatc
    16861 ggcaatacat taggtggtac attagctatc atttattttt ttgtcaaaag ctattttcac
    16921 aatgattttt tggtagaatt gatcttgctt cctattctag ttattattgt gatcgtcgtg
    16981 tcagatggta ttaacaataa ttccggtatc atttccgcaa tcgctacgtt gttgctgatt
    17041 gcgttgagca tccctcaagg cgaatccgct ttgtatgcta tccaacgtgt actagatact
    17101 tttataggga cattcattgc aatcggtatc aatttctttc ttcgtcctcc tgaaacagaa
    17161 aagaaggaag aactatcaga agatttggtc gaactccaaa agaaagaacg attgctaaga
    17221 acaaatttac aagagataga agctaagatc caaaaacaaa agaaaaatag cgaggactaa
    17281 ttttcttttg caataaaata aaaaaatgga gagaagaact ttaaaaagtc ttcctccatt
    17341 tttgattgaa cggtgaagtg tactatttta ttcttcattc atcgagtttt gttctgcctc
    17401 ttttggcgct tcatacatat ttacttggct agtagtccca cgtgtcgaca aattctctac
    17461 gatttccttc aagtcaagtc ctgttgtcgc ttttagtgtt tcttgagtag aagaaagcaa
    17521 atttgtggca taatttgtca ctcggttcgc tccactggtt tctcctgaac tgcttcctgt
    17581 atcgacaaca gagatttttt cgatatttcc tagcggctgt gctgcttcgc gcatcaattg
    17641 cggtagcata tcgataacca tactcaagac agctgcttcg ccatattcct tgaaggcatt
    17701 ggcgatcttt tgttttgctt cggcttcggc ttttcctttt gctagtgctg cttcggcttc
    17761 tgcttgtcca gctaaacgtg tcttattggc ttccgcttct gccagtgctt ccacacggaa
    17821 tcgttctgct tcggcttcag tcacttcttt gactttttga gcttgtgctt cttgttcttt
    17881 agcgtaccga tcggcatctg cttttttctt cacttccgaa tcatattgct tttcacgacg
    17941 tgtgatttct ttttcttcca gttcgatttg tttttgacgt tctacgattt taatctgcat
    18001 ttcctgttcg attacttgtt gttgtgctcg ggcactttct aaattgtagg cttggtctgc
    18061 ttttgcttta gcaacatctt gttcttgttt gtaggttgct aatttcagtt ctttttcttt
    18121 taatgattct gcgatctctg tttgtcgctg taattcagct gcttgtgatt ctttttctgc
    18181 ttgagctttt ttgatacgcg tttcttttag agcttccgct tctgctatat ctgcatctct
    18241 tttgacttgg gcgattcttg gtttccctaa agagtcaaga taaccgtttt tgtctctgac
    18301 ctctttgata gtgaaagaaa cgatgatcaa ccccattttt gcaaggtcta cagaagcgac
    18361 ttcttgtacg ctttgactga acttatcgcg attttgatag atttcttcta cagtcataga
    18421 accaagaatc gagcgcaagt gtccttctaa tacttctctt gcttcatttt ccagttcttc
    18481 tcttgtcttg cctaaaaact gttctgctgc tgtagcaatc tcttctacag atgaaccgat
    18541 tttgatgatc gaagtaccat cacacatcac cggtacgcct tgttccgtat acacttcagg
    18601 ggtggagacg tccaatttac tcgataatag actgatccga ttcgaacgtt gaaaaactgg
    18661 caagacgaat gcgccacctc cacgcacaat tttgattttg ttattgcttt catcaatgtg
    18721 cacatttttg cttcctaaat aacttccact gatgatcaac gcttcatccg gttttgctgt
    18781 ctggtacttc gctacaaaga cgatcagcaa catcagtaaa atcaaagctg cgattgcgat
    18841 cgcgataaaa acaactggta tttccattta acacacgctc ctttaagctt ttatcatgct
    18901 ttcataaggc atgacataac agattctttc cttgatctca atgatcaata ctttcgttcc
    18961 ccgctcagct gtctgttctt gggggctgta aaaacaagct ggacgactaa tggaaccggt
    19021 aacacttttc aactgaattt ctcccatacc tcgtacaggg atcggtgtga tcacaacagc
    19081 tacttgtcct tccagagttt tttcagaaga cgacatcgtt gattcggcat gtttcatcgg
    19141 catcaatata tagaaattaa gcaaaaatac caacactatc gatatgcctc cactagcaat
    19201 aaataaaaat aaagatgatt gatctgttaa acgctcacct agaaaaccaa acaagcttgt
    19261 gaaagttaac caagggataa taagcatcgg atcaagcggg ccatcaaaat cgaatacatc
    19321 tccaaaaata atcaatagga ctgccaatcc tgcacaaatc agcaatgtat accaataaat
    19381 cgtctcaagt gcgattcccc ctatcatata cacgcctcct tccatttcta cgtctatcat
    19441 accaatgttt gaaaattctg tatagcttct tttaccgact cagtggtgtt ttttatagga
    19501 aggttgtgtt tatcgttgac aatttgttaa atttttgttt tcatgttaaa attttcacaa
    19561 taaattcttt cgaggtagcc tatgtttcct ttttttaaga aaaaaccatt aaagctccag
    19621 aacacgatca tccttgtcgt ttgtttctcc acattcgttt ctttgattgt ttctgccata
    19681 ttgatcagga attttattct ttcgatgaac cgatctagtg tgtcagataa aatctctagc
    19741 attgctaaaa tcgttgccaa tgataaactc atccaaaaaa agctgcagac tggtgaccca
    19801 acacatgcga ttcaaggaat aacaaatgaa atcatgattg catcgaattt agattttatc
    19861 gtggtcatgg accctgaagg cattcgatat tctcacgcag acccaagtta catcggtctt
    19921 cctttctcaa gtcatgaaga tgtgaaaaaa gtgattggag gacaagaatt tttctctgaa
    19981 aaagaaggaa tgttagggtt cggcttccgc tatttcgttc ctatttatta caatcaagag
    20041 attattggtg cagtttgtgt tggcttaacg aacaacacga ttgacaatca agtcaaagat
    20101 ggacaaaaaa tgattgtttt ttcattgatt atcggactta gcgtaggcat cataggcgcc
    20161 gtttttcttg cccaaaaaat gaaaaaagta ctgcttggac ttgaaccaca agaaatagca
    20221 gaacagttaa gtgaaaaaac gattatcgaa aatgaagtag atgaaggaat cttagccatc
    20281 tcttctgata aaactatcct cttgatcaat acacctgcaa aaaacatttt ctaccaaaca
    20341 gagcagctat tggatttcga agtaggaaaa aaaatcagtg ataatctata tgacgttttg
    20401 tttaaagaga cgattgagca tcaaacgacc gttcggactc aatccatgta tttaaatggg
    20461 atagaaacta tttctagcgt tacacctctt tttagcaatg accgtttttt aggagcagtc
    20521 gcaactttac gaaatcgatc agaagtaatg aaattaatgg aggagcttgg ggggacgaaa
    20581 caatatataa actccatgag agctcaaacc catgaattca tgaataaact tcatgtcatt
    20641 tctggtttga ttgatttgaa gaagtatgaa gaagtcaccc attttatcca gcagctaaat
    20701 caaaattata aaaaagagat tggccaagtc tcaagtctga tcaaagttcc tgcaattgcc
    20761 ggttttttat taggcaaaat aaacgaagcc aaagaacaat ctgttgaaat gctgttagaa
    20821 accgattctt ttatccctaa aatggagatg gatgactcca tccattcact tttgcaagtg
    20881 ttagggaact tacttgataa tgcaaaagaa gccgtgctac aaaaacatga aagaaacgga
    20941 caggtccgga ttcatttaaa ctatgacaca gaagctgaag tattcatcgt gaccgtctgc
    21001 gatgacggtg tcgggatatc agaagaaatc tcagaaaaaa tatttacgct aggtttttcg
    21061 acgaagggaa ttaatcgtgg gtttgggttg aatctcatcc attcaatcgt caaacgccac
    21121 ggtggatttt tagaattttc caaacaaaca aatggcggta caatcgtcta tctagaatta
    21181 ccaaaaaagg aagatcaaaa tgatgacaaa tgtcctgata atcgaagatg atccaatggt
    21241 tgcagaattg aatcgtactt ttgtggaaag catccctaat atgaacgtaa tcgaaagttt
    21301 cagaaatgta gaagaaggaa aaaaatattt cagacaaaaa aacgcagatc tgctgctgct
    21361 tgatgtatac atgcctggcg aaagtggtat ccatttttta tcttggctta gacagcaagg
    21421 aaatgatatc ccagtgattt tgatcactgc tgcagatgat atcgaaacca taaagaatgt
    21481 gatccgctac ggtgccttag attatttgat caaaccgttc actttgcaac gttttcagct
    21541 ggcgatcgaa aagtatcttg ctttctctca gacaatccaa acgactgaaa aagccgatca
    21601 gaaattgatc gatcaagtat tcaaccacac cactcccatt cacgaaatac catcattcat
    21661 cgatttgcct aaaggtctgt taaaactgac tttaaaaaaa ataagtgcat ttattctaaa
    21721 agaagatact attttttcaa caaaagaact ggctcaaaaa agctcactgt cacgtatatc
    21781 agtcaagaaa tatttgaatt ttttggtaga acaagatata ttgaacgaaa gaatcaaata
    21841 tttggaaatc ggccggccgc taacctgtta tgaattgaca aagaatgggc ggaaaaagct
    21901 cacagattac tgttgagtca tcgaattatc ccatacaaaa tagtccaagt atacagaagt
    21961 aacaactgtt tacttggact atttttaaac attttattga cgaatatcca tgttttctta
    22021 tcttaattga cgggctttag gaagagatac ctcatttttc gtttttccag tttctattat
    22081 ttcacgaaag ttctcgaaac ttgtcgaaat catatttgat aatgcctcat ctgtatttga
    22141 acctacatgc ggggttacaa gaactctagg gtacatttct actaattgct ggatagcagg
    22201 gtctggaatc gtctcccatg gcttgaagga tcggaaaaaa acctctgttt catttgcaaa
    22261 aacatcggtt gcaaaaccag ctaaatgatt gcttttcaaa g
//
LOCUS       DAMEET010000045        22218 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.046, whole genome shotgun sequence.
ACCESSION   DAMEET010000045 DAMEET010000000
VERSION     DAMEET010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.046"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            170..514
                     /locus_tag="PB780_001531"
     CDS             170..514
                     /locus_tag="PB780_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDE0733588.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            515..934
                     /locus_tag="PB780_001532"
     CDS             515..934
                     /locus_tag="PB780_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDE0733589.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1026..1376)
                     /locus_tag="PB780_001533"
     CDS             complement(1026..1376)
                     /locus_tag="PB780_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733590.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1342..2673)
                     /locus_tag="PB780_001534"
     CDS             complement(1342..2673)
                     /locus_tag="PB780_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HDE0733591.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2929..3495
                     /locus_tag="PB780_001535"
     CDS             2929..3495
                     /locus_tag="PB780_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDE0733592.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3640..3727
                     /locus_tag="PB780_001536"
     tRNA            3640..3727
                     /locus_tag="PB780_001536"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3672..3674,aa:Ser,seq:cga)
     gene            4061..5035
                     /gene="bsh"
                     /locus_tag="PB780_001537"
     CDS             4061..5035
                     /gene="bsh"
                     /locus_tag="PB780_001537"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HDE0733593.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5360..7255
                     /gene="asnB"
                     /locus_tag="PB780_001538"
     CDS             5360..7255
                     /gene="asnB"
                     /locus_tag="PB780_001538"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDE0733594.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7450..7896)
                     /locus_tag="PB780_001539"
     CDS             complement(7450..7896)
                     /locus_tag="PB780_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HDE0733595.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8124..8273
                     /gene="rpmG"
                     /locus_tag="PB780_001540"
     CDS             8124..8273
                     /gene="rpmG"
                     /locus_tag="PB780_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDE0733596.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8361..8879
                     /locus_tag="PB780_001541"
     CDS             8361..8879
                     /locus_tag="PB780_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDE0733597.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8956..9912)
                     /locus_tag="PB780_001542"
     CDS             complement(8956..9912)
                     /locus_tag="PB780_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDE0733598.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10078..10884
                     /locus_tag="PB780_001543"
     CDS             10078..10884
                     /locus_tag="PB780_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDE0733599.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10881..11870
                     /locus_tag="PB780_001544"
     CDS             10881..11870
                     /locus_tag="PB780_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDE0733600.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11867..12871
                     /locus_tag="PB780_001545"
     CDS             11867..12871
                     /locus_tag="PB780_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDE0733601.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13003..13545
                     /locus_tag="PB780_001546"
     CDS             13003..13545
                     /locus_tag="PB780_001546"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDE0733602.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13565..14263
                     /locus_tag="PB780_001547"
     CDS             13565..14263
                     /locus_tag="PB780_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDE0733603.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14284..14496
                     /locus_tag="PB780_001548"
     CDS             14284..14496
                     /locus_tag="PB780_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HDE0733604.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14496..15458
                     /locus_tag="PB780_001549"
     CDS             14496..15458
                     /locus_tag="PB780_001549"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HDE0733605.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15476..15871
                     /locus_tag="PB780_001550"
     CDS             15476..15871
                     /locus_tag="PB780_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDE0733606.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16034..16399
                     /locus_tag="PB780_001551"
     CDS             16034..16399
                     /locus_tag="PB780_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HDE0733607.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16396..18207
                     /locus_tag="PB780_001552"
     CDS             16396..18207
                     /locus_tag="PB780_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HDE0733608.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18269..18436)
                     /locus_tag="PB780_001553"
     CDS             complement(18269..18436)
                     /locus_tag="PB780_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HDE0733609.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            18684..19673
                     /gene="galE"
                     /locus_tag="PB780_001554"
     CDS             18684..19673
                     /gene="galE"
                     /locus_tag="PB780_001554"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDE0733610.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            19685..21169
                     /gene="galT"
                     /locus_tag="PB780_001555"
     CDS             19685..21169
                     /gene="galT"
                     /locus_tag="PB780_001555"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HDE0733611.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            21193..22173
                     /locus_tag="PB780_001556"
     CDS             21193..22173
                     /locus_tag="PB780_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDE0733612.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
BASE COUNT         7208 a         3806 c         4620 g         6584 t
ORIGIN      
        1 aaaaaaagcg ttagcaacta tgcttaaaaa gcatatcgac tgaataaact aagcattgga
       61 caggctgaaa gactacattt aaaatgaaat gcgaagaaac aatagaaaat aaagttgcct
      121 tagaaccata gttgaatcac ctaacagtcg tgaagggaga ataagagaaa tggcagaaaa
      181 acaaaccgca ggacgagatc aattaggaga ttttgcacca aaatttgcag aattgaatga
      241 tgatgtttta tttggacaag tatggtccag agaagaatta tctgctcatc agcgtagtat
      301 catcaccata tctgcattaa tttcaggagg aaattttgaa caattgccag ctcatctgaa
      361 gattggcaag caaaatggca ttactagtga agaaatagca gaaatcatta cccatttagc
      421 tttttatgta ggatggccaa aagcttggtc agcttttagt ttagcaaaag aaatctattt
      481 agaagaaaat agcgaagata agaaagaggc gtaaatgacg acaaaacacg aagaaataca
      541 caatggcgtg atttttccag caggtgaaaa aaacgaagct tacgctcaag tatttaccgg
      601 gcaaagctat ctgaatatgt tagtgaatga tcccgaagtt tctgttggtg taggtaatgt
      661 aacttttgaa ccaggttgtc gcaatcactg gcatgtccat aaagacggct atcaaatctt
      721 acttgtgacc ggaggagaag gctggtatca ggaagaagga aagccagcaa gacgattaat
      781 ggctggagat gtcgtcgtaa cgcacaaagg gattaaacat tggcatggtg cgacaaaaga
      841 cagttggttt gaacatttgg cgatcactgc tggtaattca gagtttttag aacctgtaac
      901 agatgaagtg tatgaaggat tggaaaatca ataatgtcaa gcttgaacag ctcctgaaag
      961 tagattttaa tcgaactttc aggagctgct tttttgattc agtcttttat aaatagaaaa
     1021 tagggttaat aataggaatg agaagcacgt cgaaagatct taatattgat aatcggctgg
     1081 gcatacatga tttccacgtc atcaatcaaa ctacctaaca gatgataatc ttcccgatca
     1141 tgtcggggat tacctaatag ttcttgataa tagatttcca tactagggtc ccgaggattg
     1201 ttcagcaaat cagcaagtac atctaaatct tctggctgag tttctgccgt accctgtatc
     1261 tctatgaaaa gcccttcctc agcaaattct actccaatac gtatttcagt tatttcatgg
     1321 gaatataaat aagtaagaag ctcatcgatg attctttttg ataattcttt ttccaagtgt
     1381 ctttttttcc tttctgacag cggtaaagtt atctagtatc ggtacaagga tacctgcaac
     1441 gaaaccagtt gaaaaaccac tgttatatag atttagtcct ccgtgcaaag tgctgacgtt
     1501 atggacaaga gcaatatgta gtacaccagc tattacaccg taaaaactgc cgtaaaaacc
     1561 gcttatgggc gcgagacctg tcccaaacac agctgcaaca agcatgccag tactggtgat
     1621 ctcatgaaaa gttccaaaca aagaagcaat aaatattccg actagtatcg gaaaactgtt
     1681 ttttaaatga caaccaaaag cactgaaacc caccgcagat aaaatagcac cgatgacagg
     1741 tccatttaac tgaccgccaa ccagcaaaac ataagaagac agcatcagac caactaagcc
     1801 tatgttgatc aaagtagcac caataccaga attagcaatg aaatcagttg tcaattttcc
     1861 agatgaatcg aaaatttctc gaatccccaa aagccgaaag gaattataat agaaaccaat
     1921 tccgactgtc agcgaaaaaa agccaaaaat aatccaaatg agaggaaagt gatattcatt
     1981 gaaaaccaat gtattttcga cgattgaata gctgaataac ctcaaaacag cagtaaaagt
     2041 catcgcaatc aacccagagg tgaatccaat attataaagt gtgaatccac gatggaaaac
     2101 taaagtttga gcagctaagg gcggaagaag tagaccaatt gctatgccag ctaaattccc
     2161 caataaaata cctatcagta aaggaaaacg cataccaaaa gtgatataac taacgaccgg
     2221 tgataatgcg ctaccaaaca gtccgatcat gatgtactga gaaaaaggtt tattgacaaa
     2281 tacagcgtat agatacacac ctaaaatgat tgagatagaa ttataaaggt ttttgccaaa
     2341 aaaagagaaa ccggtaactg ttagaatcga agcaaataag ggaccgttta aaatcacctt
     2401 gtgccgataa gctaaaagca cagaaagtaa ggtcaaaatc cctacattta tgaatgcgct
     2461 tccgaaaccg cctaaagcaa aataatctgt caaaagattg cttggcgagg taagaatagt
     2521 tagcaatcca ctccaattat cagtaggagt attagaaagc aagccaataa aaagaaaaaa
     2581 aacacctgta aaaccaagat aaaaatactt tcttttctgt gaaacgattc gtttttgagg
     2641 atgaatataa ataacgtcag tttcgttttc cattacacac cttctttttc attttcttta
     2701 actataactt gaaatgaaag atttggacaa ctatcattta ggtcaaaact aaaaatgtaa
     2761 gggaacccat tgaatttaac caaatttttt taattttgca aaaaaaagtt aaagaagact
     2821 gaaaaaaaat ttaaaatata acgttttgag taaaatgttt gcaaaaaatg ctaaaaatag
     2881 ggtacaataa aacaacatat actgcagtaa agaagtgggg gagttttaat gagaagaaaa
     2941 gtctatacca aggatcaaat tctaaaagct gcttatgatg ttgttgcaaa ggaagggttt
     3001 aaaggattta ctgcgagaaa tattgctaaa aagatgggga tttcgacaca gccgatttat
     3061 ctggaattca aaaacatgga agatttaaaa aatacgttgc tggattctat ctttgaacaa
     3121 ctaaaagaag atgttttccc tattgaacgt actggtgata aaatcattga tctcggtttg
     3181 aactatatct actttgcaaa aaagcatcat agtctttaca tggcattgta cattgatgaa
     3241 catggggacg gaaagaaaat gcatgatttt tcatacaatt atttccgtga aatagttaaa
     3301 gaagatgaaa gatacaatca attatcggat gaatatgtcg atgctctgca tactggtaca
     3361 tgggtagcag tgactggctt agcgtcattg atgtcatcaa acatcatgca tccaacagaa
     3421 gaacaaatca ttgcactgat caaacggaca attgactcga ttttagcatt agataatcct
     3481 gaaataatca gatagtgttc tatcagtatg aatttgatcg gtaagtccgt tggctgcaaa
     3541 ctgtcaatgg acttttttgc taaaaaaagc gatttctgtt atttatctat tagaaatagg
     3601 cttgacattt cgtgaaaaaa gtttaaaaat agtacttacg gaaggttggc agagtggtaa
     3661 tgcaccggac tcgaaatccg gcgaaccggc taataccggc gcgcaggttc aaatcctgta
     3721 ccttccttat atcaacgatt agaggttgtg aagaagctgt cctgcatcaa gtaataagaa
     3781 cggccaaaca aaaatagctt ttcctatttt ttcgtttgat tgggcttatt accgcagatg
     3841 caagcttttg aacaccgttt gttcggaata aggggctgtg acaagacttt tgtcacagcc
     3901 ccttattgtt gtacttttaa cctacaatat tttttgattc cccctgagta ctatttttta
     3961 taaatgctag taaatcaatc ttctttggcc tttttttgat aaaaaaaata ctagatgtaa
     4021 gataaaagtg taaaaacgat ttgttttagg acgggaaacg atgtgtaccg caatcactta
     4081 tgcaacgaaa gaccattatt ttggaaggaa ttttgattat gagatgtctt ataatgaagt
     4141 ggtcactatt actccaagaa attatcgttt cgattttaga aaggtaaaaa atttagataa
     4201 acattatgca atgataggaa tagcagctgg ggtatccaac tatccactct attatgaggc
     4261 tacaaatgaa aaagggttga gtatggcagg actgaatttt cctggcaatg ctgattataa
     4321 agaattacaa gaaggaaaag ataacgttgc accatttgag tttattccat ggatactagg
     4381 gcaatgctca accatagacg aagctaaaga attattggct actatcaatc tagtcaatat
     4441 cgattttagt gaaaaactac ccttgtcacc tttacactgg ttattagctg ataaagaaaa
     4501 atcgatagta attgaaagta tgaaagatgg acttcattta tatgataatc ctgtcggtgt
     4561 gttgacaaat aatccgccat ttgattatca attatttaat ctgaacaatt atcgctcgct
     4621 atcaaatgga acaccagaaa atcatttttc aaaccagatc agcttgaatg tttatagtcg
     4681 tgggatggga ggattgggct tacctggaga tctctcttct gtttctcgat ttgtaaaagc
     4741 cacatttact aaaatgaatg ctgcatcagg tgattcagaa tctgaaagta tcagtcaatt
     4801 ttttcatata ctaggctcag tcgagcaaca aaaaggagta tgcgatactg gagaaagaaa
     4861 atacgaatat acgatttatt cttcttgctg taacgttgat aaagggattt attattatcg
     4921 tacgtatgaa gacagtcaaa ttactgctgt aaatatgaat aaagaagact tggatcgtca
     4981 tgaattggtc agttatccag tcataaaaga gcaacagata aactatgtca attaattaat
     5041 gaatagatcc gtttggtatc atattttctg atacagtcat aattagacac gatctttagg
     5101 tcgtgtcttt ttttgctggt ttttttggaa atagcatcca ctaatatttt tttaacacga
     5161 agagaataag gtagcctcaa aaaagaaaac atggtaaatt agatattggg actttatttt
     5221 ctgtaaaaac agaaactttc atcttttgtt ttcacgtgac gaaattgtaa gagagaaaat
     5281 attcgtccgc tatttattct atgataagat gcatatgaat gaaataaaca gtaattttat
     5341 attaggatgt gaaagttaaa tgtgcggtat tgtaggtttt gttaacgaca aggacaacaa
     5401 aaaaacaatt attaatacaa tgatggatcg aatcgttcac cgtggaccta atagttcagg
     5461 agaatatatc gataaacatg ttgctttagg attcagaaga ttaagtatta ttgacttaga
     5521 gggcggtact cagcctattt acaatgaaga tcggacaaag atcattattt ttaatggcga
     5581 aatctataat taccagcctt tgagagaaga attgattgca gctggccacg tttttcaaac
     5641 acatgcagat acagaagtcc ttttgcatgg ttacgaagaa tggggaacag aattattgca
     5701 aaagatccgc ggaatgtttg cttttgctat ttgggataat gaaaagaatg agttattcgg
     5761 tgcaagagac cactttggga tcaaaccata ttattatgca gaaatgaacg gaacatttat
     5821 gtttggttca gaaatcaaaa gttttctgcc tcatcctgat tttaacaaag aattgaacaa
     5881 agaagcgttg aaaccttata tgactttcca atattctcca ttaaacggcg aaacattctt
     5941 caaagatgtc tatcgtttgc ctgaaggcca ttattatacg tataaagatg gaaaaataga
     6001 cattcagcaa tattgggatg cagattttga aacaaaagaa acgcattccc gacaagaatg
     6061 gatcgaaaaa atcgacgaaa ccgttcaagc ttctattgaa gcacatacgg tcagtgatgt
     6121 agaagttggt tctttcttat ccagcggggt tgattccagt tatgtaacct ctgtattgaa
     6181 accagatcat tctttctcaa tcgggtttga tgataaaacc tataatgaag cgattgaagc
     6241 cagaaaacta accgaactat tggacttaga taatacagca gcagtgatcg acggagacat
     6301 gtcgttcaaa gcattccctt tgatccagta tcacttagat gaacctgatt caaatccttc
     6361 ttgcgtacct ttgtactttt tagcaaatct tgcttcacaa agtgtgcggg tagtccaatc
     6421 tggagaaggg gcggatgaac tgtttgcggg ataccaaacg tatggattcc atacgaattc
     6481 aaaattcatc cgtgtgattg ctcaaggatt gaaaaaatta ccaaaaggaa cacgctataa
     6541 tttaggacgt aaaatcggaa aaatgaaaaa tttccatgga cggatccatt tatatgaatc
     6601 acttgctcct gcaaaagaat attttatcgg tcatgctcga gtgttcgaag aatcagaagc
     6661 atcagaagtg ttaacaccaa aatatcaaac agcaccatct gtggatgaaa tcatgactgt
     6721 tcattacgag aaaacagaag gaatcaaaga tgaagtcaat aagatgcaat atgtcgatct
     6781 tcatcaatgg atgccaaaag atatcttgtt aaaggcagat aaactttcta tggcaagttc
     6841 tttggaagtc cgagtacctt tattggatat cgaagtcatg aaattggctc aacaaatacc
     6901 aagtaagttt ttattgaatc aaaacaacac aaaagatatt ttcagacaag ctgcgaacaa
     6961 acatcttccg gaagaatggt cgaatcgtgt gaaattagga ttccctgtgc caatcaaagc
     7021 ttggttgaaa gaagaacacg gctacgaaca agtcaaagca ttattcgaag cagattttgc
     7081 aaaagaattt tttgatcaag aaaaaatcat gaagttgcta gatgaccatc acgaaggacg
     7141 taaagaagag caaagaaaaa tctggacaat cttcagtttc ttgacatggt ataaagtcta
     7201 ctttgtagat gaaacgattc cgcaagcaga agcaatcgac tatgtgactg tttgatgaaa
     7261 atcaaatatc aataaaaaag aggttttctc ctaccgttta acactgagtg ataagaaaca
     7321 atttcaaaaa acagctcttc ccgttttata gatattatga cttatggttc ggagttggca
     7381 gaggaacacc attgtttcga acataagggg gttgtgacac gattttcgtc acaacccctt
     7441 tacatatgtt caataatcga tttctttttc ttttgtccca tcttttttat aaatgaacaa
     7501 agttgattct ttgttttgtg ctacttgctt tcctttttcg attgcttctt tttttgtatc
     7561 aaaatggttg cttgcctttt ttgccccttt agatttcacg atccattgtt cttcctcata
     7621 ttctacgatc acatctgaat caagcagttt tccagcacga ggattcgtat cgtgttcatc
     7681 cgtcttgctt ggattttttt ctttttcgaa agttcgtttt tctgattcac tagcatcttc
     7741 ataccatttt tttgcttgat caatggcaat tgggatcgca cggttatcag ggtacccttc
     7801 atctaataat gcatttgcga tgtcgattgc tttttttctt gtcaatttat ctaagttttt
     7861 cattgatgca ggaaagtcct tcatgttcca tggcatatta gtatccccct ttattaaaaa
     7921 acataatcta tataaacaag tgtaagggta tttatacaaa aagaaaaaga ttttgtttga
     7981 gaagtaaaac cgttgtattt atcgtcaagt agttttactt aacaaaatcg tgaaaaacac
     8041 tagcaatcta gaaaaaatca tggtaagata atcaagtctg agagtcatct tagaggattc
     8101 ttgtaaaaat gggagggaaa aacatgcgcg taaacatcac tttagagtgt acttcttgta
     8161 aagaacgtaa ctatctaaca agcaaaaaca aacgtaacaa tcctgatcgt ttggaaaaac
     8221 aaaaatattg cccacgcgaa cgcaaagtta ctttgcaccg tgaaacaaaa taatgttttt
     8281 aaagttctca attccttgct ggtcaaagaa ttgagacttt ttttatctca aaacatctaa
     8341 aatcataagg agcggataat atgtttattt ccatgtgggc acaagataaa aatggtctga
     8401 tcggcaaaaa tggtttgtta ccatggcgat tgccaaatga tatgcgtttc tttcgcgaac
     8461 acacaatgga tcgaatttta gtgatgggaa gaaagaccta tgaaggaatg ggggatcttt
     8521 cacttcctta tcgccatatc atcgtgttga caacgcaacc aaattttcgc acgaaagaaa
     8581 atgcggaagt aatgcattcg atcgatgaac ttttgtcgta tagtcaaacg atctcagaag
     8641 atatctatgt ttcgggtggt agtcggattt ttcaagaact tcttccttac actggtcgga
     8701 tctggcgaac attgatcgat agcacgtttg aaggcgatac gtatattggg aatattgatt
     8761 tttcagactt cgcccttata gaggagtatg aggggataac ggatcaagaa aatctatatg
     8821 cccatcgttt tcaaaaatgg gaaagagtaa aaaatagggt tgcttcaaaa gaagaataaa
     8881 atctatgcgt ctagtttgtt aataagaaaa aaagcatctc acgactaatc gtgaagatgc
     8941 tttttttagt taaaatcaat ttagtttttc tttgatatca ttgaccattt cctgactggc
     9001 aataggtccg ttatatagcc aactagattc aggtccgaat tgcagtacat gattgtctgt
     9061 cactgctttt agattcttcc agcttggttc attgaacatg gcagcatctt tgtcactgtt
     9121 cacaaggatg atgtagtcag catctaattc agctagcttt tcagaggaaa cagcagacca
     9181 gtcagaagtg gcttttttgg agacctcttc cactagatca ggaacaccaa attccagatc
     9241 gtcgtatacg attcttccac ttgaccgatt tttgctgacc ataaaggctg agttgttggt
     9301 cacccacaac actgcagctg tcttgttctc gattttatct gctagttctt ctcgagtttt
     9361 cttgatggat ttttgataat cggaaatgat tttttctgcc tctttttctt ttgccaaagc
     9421 tttgccgaca tcttttagtt gttcttccca tgtgacatct gttccgtttt ttacaacata
     9481 agttggcgcg attttgctat attgttcgta tttcccgcct tcgacagtag cggaagaaga
     9541 aatcaacagc aaatcaggct caaagcttaa cactttctca taaggaaggt cataagaaat
     9601 cgttgggaca tcctttaaat catcttgcag ataatcttga atggaaccac tgccaacagt
     9661 ccactgggcg ataggttttt cccctagtgc aatcagatag tcttctaagt aactgccgat
     9721 gatccgtttg ggcgcgtttg gaatctctac tttatgtcca agtgtatctg tgactgtatg
     9781 agtactattg gatgaagtcg tcgaattggc agttgtagct gtttctttat ctgaagaggg
     9841 ggagcaagcg ctcagtactc cagccagcag caatatgcta gataatgtga atacttttgt
     9901 ttttaatttc atttctttcc tcctaacata attgagaatc tttctcattg gttattattc
     9961 ctgatatttc gaacgctgtc aaccgcttca tttggctata gatatgaaag tagcgcttga
    10021 cgaaatgttg aaacagcgtc ataattgaga atgattttca gcgaaaggat gggtgaaatg
    10081 acgagattaa gagcagctga cattgcagcc ggctacgaaa acaaaaaaat cattgaaaac
    10141 atgtctgtta cgattcctga agggaaaatc actagtttga ttgggccaaa tggaagtgga
    10201 aaatctactt tattgaaaag ttttacccgt atactacctg tgaaatcagg aaagatcatt
    10261 gtcaataact ctgatatctc tacatacagt tcaaaagaac ttgcaaaaaa aattgcatta
    10321 ttagcacaaa ctagtgaaca ccctacaggt atgacagtag aagaagtagt atcttacgga
    10381 cgataccctt atcaaaaact gttcagtggc atggacacgc aagatcatga agcaattgag
    10441 tgggcgttgg atgctacagg tctagctgga tttcgtatgc gggatatcgc aacgctgtct
    10501 ggtggacaaa cccagcgtgt ctggatcgct atggcgcttg ctcaggaaac agatatcttg
    10561 atcttggatg aaccgacaac atttttagat ccggcacacc aattagagat cctacagtta
    10621 ttgactgaaa tcaatcaaat gaaacaaaca acgatcttga tgtcgattca tgacattaat
    10681 catgcttcac gtttttcaga ttatttatta gcaatgaaaa acggtaagtt gatcacagaa
    10741 ggtacacctg aagaagtgat cacaaaagag tgtatgaaaa aaatatttga gattgatgca
    10801 acgattgtca cgcttccaag aacaaataaa ccagtttttc taacctatga tttgttgcca
    10861 aaagagcagg aggatcaatt atgaggacta tgaagaaaaa tagactcctg cttttggtct
    10921 gttttgttct tttgattatt ttttttgttg ctctttcaaa tggcgcagcg actgtgggaa
    10981 aagcagattt ttggcaggcg ttgtttgatt ttgatagatc aaaacaaagt caccaaatca
    11041 tacgaaatat tcgtttgcct cgtgtggcgg gagcatttct tgtagggagt tgttttgcat
    11101 tgagcggtgc attgatgcaa ggagtgacaa gaaatccttt agcagattca ggcttactag
    11161 gaatcaacgc gggtgcttcg ttggcaatgg cccttagttt tgctttttgg cctaaagctt
    11221 ctgatttttc tgtgtttcta atgtcattgg caggttcttt tgctgtaact ttgctcgtat
    11281 ttggtttttt gcagttttcg aaaatgggca tgaatccttt acagctgatt ttagctggcg
    11341 tagctattag ttcttttttt actgctatca gccaagcact tacccaattc tttaatctgc
    11401 agcaggatct tgctttttgg tttgtaggcg gagctgccaa tatcacatgg aaacaattag
    11461 cagtattgtt tcctttatat atacttgctg ttttggtttc tttttcatta ggaaaagcaa
    11521 tatctttgat tcatttgagt gaaacacatg cattagctct tggaagtcat cctaaacgaa
    11581 cgcggttgat tgttttctgt ctagttgcag tgttagcagc cattgccgtt tcgttagtcg
    11641 gacctgtttc atttattggg ctgttggttc cgccagttgt tcgcggaatg gttggaacgg
    11701 actatcgtta tgttctacct gtctcttttt tagttggcgg tggattggtt atgttggcgg
    11761 attttacagc gaggatgatc aatccccctt ttgaaacacc ttttggatta gtcattgcat
    11821 tgattggcgt tccgttttta ctgatacaag taaggaggga acaacggtga aaaaaatata
    11881 ttggacgttc tatttcttgc tttttgtact tgtcatcctg tctataggta gtttaatgat
    11941 tggtacgcct tttatttacc tggatcagct ttttgccacg ttcataggga aagaatcatc
    12001 ttctttggcg ctgattgtta gagaatttcg agttcctcga ttactgatca gtttattagc
    12061 aggagcttgt cttggcgttt ctggttatat cttgcaagga gtaacaagaa atgaattggc
    12121 agattctagt atattgggca ttaatgccgg agcaggattt ttagtgatgc tttatttagg
    12181 tttcttttcg cactattcgc tgccgtttct attgctagtt gtagcctttc taggtggtgt
    12241 tttagctgct tgctgtgtct atcttgcggc ctatgatcga aatggctttt tgggtatgaa
    12301 tcgaatctta ttgtccggta ttgccgttaa tgccggcctt tctgcattaa cattgctatg
    12361 cacgatccaa ttatctaagg aaaattatgg atttgtgaat gcatggttag cgggatcaat
    12421 ctggggtgcg agctggcctt atgtttgggc attgcttcca tgggcggtta tcttaattcc
    12481 gctaggagga ctttatgcac agaggattgt attgctatca tttggtcaag aacgcgcgca
    12541 aggattaggc cttaacgtgg ctaaaagtca aaaaatcctt ttgttgttag ctgttgcttt
    12601 ggcaagcggt agtgtagcgg tgacaggtag tttatctttt gtaggactac tagctcctca
    12661 tgctgcgaag ctagtcgtac gtaaagaaaa taactggacc tttgttctga gcggtttgtt
    12721 cggttccatt tttctgctta gtgcagatat ggttgctaga gtgattctac taagtgggga
    12781 agtacctaca ggaattttga ttgccctttt tggtgcgcca tattttctgt atgcactttt
    12841 tatcagacaa aaacacgcgc tgaacagttg aaattcatta aaaaaggaag gccaatcatt
    12901 tctaggtctt ctttttattc tatttatgca tttatttgaa agttcgattg ctatttttct
    12961 ttttttccta taccatttta tgagagaagg aagggtgagt ttttggacca attgaaagta
    13021 agattacgcc ggaaaggaat cgaaaagctg aaaaatctgg ccaaaagtgg caagaaaaag
    13081 gaaaaagaac agcgaatcta taaacagcta ttcgccagcg agtactggaa gtcagcaaaa
    13141 gtgataggga cgactttatc gaatgaattt gaactggata cacagccggt tatccaacga
    13201 gcagaaaaag aacaaaagaa aatagttgta cctaaaactt tgccgaaacg tcagatggcg
    13261 ttttatgaaa tggatgaaca gtccgttctt gtacgttcga gttttggtgt tcttgagcca
    13321 caatcagaga agctctattt gccggagatg atcgatctcc tcattgtacc aggtattatc
    13381 tacaatagag ccggttatcg aatcggtttt ggcggtggtt attacgaccg gtatttaaat
    13441 aattttcata aaaaaacatg tagccttgtt tttcgtgaac aattgaatga acaatggcaa
    13501 ccggagagct ttgatcagaa gatccaacaa ttgtttattg actaaaatga aatgggaggt
    13561 tttcatgaat tatcaacagc aaataaaatt gcgtgcatgg ttaagacgac cgttatggac
    13621 ctatgctttt ttagggatac agacagcggt cttcctcatg atggaattat ttccgcgact
    13681 tgagatccct tattatgccg gaatgtatgg tccttatctt gttcacttca atgaatggtg
    13741 gcgtttagtg acaccaatct tcatccattt tggactgatg catttcgtaa tgaactcact
    13801 gatactgtat tttatggggc agcaaatcga ggcaatctat ggccattggc gttttttctt
    13861 gatttatatg ttcagcggga taatgggaaa tacagcaagc tttgctttta acgaagcgaa
    13921 tgttctgtct ggtggtgcaa gtacttcgat attcggtttg ttcggtgcgt tgtttatctt
    13981 agggtttcat tttaaacata acccagcgat ccagcagcta gtcagacatt ttcttttatt
    14041 tattgtgttg acatttgttt ttggtcttct ggatacctct gtagatgttt ggggacatgt
    14101 cggtgggcta attggcggat tagtgttagg gaatgttttg ggtctaccga aacaacagac
    14161 aagctattcg atacaccaaa ggattctttc tacactagtt tttgtatttc tttttgtaat
    14221 atgtatttta ctgggtttaa aaaaatatgg tttacttgta taatatgaag gaagtgctta
    14281 aagatggaga gcttatatga tgtacagcag ctgttcaaaa aatttggcat ctttatctat
    14341 gtgggtgcac gcatctatga cattgaatta atgactatcg aattgcgtaa tctgtatgat
    14401 agccgtttga ttgatcatga tacatacatg acagcgtggc gcatattaaa aagagagcac
    14461 cgaattgaag aaagccgaaa gaaaggaaat ttgtaatgga caagaagatt attgggattg
    14521 atttaggtgg aacaacagca aaatttgcaa ttttaacacc agaaggagag attcagcaaa
    14581 aatggagcat cgataccaat attttggatg atggtaagca tatcgttcct gaaatcattg
    14641 aatcaatcaa tcatcgtttg aatttatata atatgaaagc cgaagacttt atcggaatcg
    14701 gcatgggtac cccaggaagc gtggacagcg aagctggaac ggttattggt gcgtataact
    14761 taaactggac agaagttcaa tttgtgaaaa aactgatcga ggcaggaaca ggaatcaaat
    14821 tcgctatcga taatgacgcg aatgttgctg ctttaggtga acgatggaaa ggtgctggag
    14881 aaaacgatcc agacgttgta ttcgttactc taggaacagg tgttggcgga ggtatcgttg
    14941 ctggcggaaa tttgattcac ggcgtgagcg gagctggagg agaaatcgga catatcactg
    15001 tggatcctga agggttcgaa tgtacttgcg gtaaacgcgg atgtttagaa actgtatcta
    15061 gcgctacagg tgtggtacgc ctaggtcgtt tcttggcaga agaatacgca ggggattcaa
    15121 aactgaaagc aatgctagac aacggagaag aagtaacaag taaagatatc tttgaaatgg
    15181 ctcaagaaga cgatccgttt gcattgatgg ttgttgatcg cgtatgcttc tacttaggtt
    15241 tagcttgcgg aaacttgggg aatacattaa atccgtcaag tatcgtgtta ggtggaggag
    15301 tatcggcagc aggagaattt ttacgtagcc gtgtagagaa atattttaaa gaatttactt
    15361 tcccgcaagt aagagaatca acaaaaatca aattagcaga gcttggtaat gaagctggtg
    15421 tcattggtgc tgcctcatta gccttgaaat ttgcttaaaa aaacggaggc attacatggt
    15481 tttatgggta attaatatca ttttgctatt gattgtgctt gcaatcatcc tttgggaagt
    15541 ctatctacga gtaatggcca aacgttcagc aacgactttg acagaagaag aatttcgaga
    15601 aggaatgaga aaagcacaag taatcgatgt tcgcgaaaaa gatgtgtttg atgctgggca
    15661 tattttaggt gcaagaaaca tcccttacac tgttttgaaa gattcattag gatctattcg
    15721 aaaagaccag cctgtctata tttacgatca aaagaaaagt ttgagcatcc gtacagcaaa
    15781 taaattacgt aaagcaggtt atcaagagat ttatttactt aagggtggat atgacggatg
    15841 gtctggaaaa atcaaaagta aaaaagtata aaacagatga ttgtatgaca gctttaaaaa
    15901 ccgaatcaat cattagattt tgaccaagat cacatgaacg gcgcaatgga tagttgtgcc
    15961 gttcattttt tttattttgt gctattatct acatatataa gctaagcgaa aaggtagaaa
    16021 gaaggaaaca actatgatca aattaaaacg tgtttacctt gatgctgaaa aagaggatgg
    16081 ctatagaata ctcgtggata gattctggcc acgaggaatt tcaaaagaaa aagcgcagtt
    16141 agatctgtgg ttgaaagaaa ttgcacctag taatgaagca cgcaaaactt ttgaccatgt
    16201 accagaacga tttgcttcat ttgcaaagaa ataccagcaa gaattgattt tcgaagaaga
    16261 aaagaagcaa gctattttta aactcagtca acttatgaaa gaacatccag tcattacttt
    16321 tgtttatggt gcaaaaaatg aacatcagaa ccatgctgtt ttattgaaaa aatttatcga
    16381 aatggaggca tgcaaatgaa aatagggatc attggtgccg gacctagagg attaagtgta
    16441 gcagaaagga ttctccataa taatcaggac agacattctc ttgaactttt tcttttcgat
    16501 cccaatggtc ctggaggtcg agtttggcgg ttgaatcagc caccggagtt attaatgaat
    16561 tctgtttctc aacaagtgac gctttttaca gatgaaacat tgtccagtgg aggattggta
    16621 acacagggac ccaacttgtt tcaatggagt aaaggacctg ctacagatta tatcgaacag
    16681 acggtaacca aaaataagca actaattata gaagaaatca atcgtctgga aaaagatcag
    16741 caatctactc gatgtttata cggactctat caacgctggt ttttctcaaa gttgcaagaa
    16801 gaatttccag cttctattca gtttgtttat tctttagtaa aaaaagtaag caaaagaaaa
    16861 gatggttatc ttttacagac agatcagcaa agctattcta tcgatcagct tgttttagca
    16921 agcggtcact gggaaaatga tgagacagaa gaagaaagag tgtgggtaga ctacgcaaag
    16981 aaaaatgttt tactgtatca gcttccttct aatcctgcag atgtagatct ttcgaagata
    17041 ccgcctaaag aaaatgtctt tttaagaggt ctaggcttaa gtttttttga ttatgtcgga
    17101 ttgtttacag tatcaagagg agggcgcttc gagaacgaaa agggaaaact ggtctatcat
    17161 ccatcaggca aggaaccgat tgtatacagt ggatcaagaa gggggctccc ttattaccct
    17221 cgaggaagaa atcaaaagca aggcggagca atggcatggc cacggttact aacaaaagaa
    17281 aacctggagc aatggcatca gtcgggattt ttgactggcg aaacattttt tgactatctg
    17341 aaaaaagacg ttgagttgtt ttattacaag aaaatcatcg aagaatatca cttgccgatt
    17401 ccaaagaaaa attttgagca tgattttctc tccctctctt cagaggagtg tctcggaaag
    17461 catccagcac taatccctta tagatgggat tggtcgtatt tagaaactcc tcttgcctat
    17521 cagaatgagc ccttttcaga agctagccga atatttattg aagggcagat aaaagaagcg
    17581 gaaaaaggca attgcacagg agcatttgct tcaacatttg atgcattaaa agactggcgt
    17641 gatccaatcc ggcaggctat tgaatggcac gttttttctg ctaaggaata caaagaaata
    17701 ctatggggat ggtttactca tctaaatagc tttttaacga tcggtccgcc aatcattcgg
    17761 acgaaagaat tagcagcact gatagatgcg ggtatttttc atttgatcga accgccattt
    17821 gatattcagc aaaagcacaa ccgctatgtt atttctctac aagataaaga aatcagcagt
    17881 cgctatttga ttgaagctcg attgccacaa acaagctttt ctcgttcaag taatcctgtc
    17941 ataaaaaatt tgaaggaaaa caaacttgtt cgcccttttt cattttcaga taatacagga
    18001 agttatcatt cagaagctat agatgttgag cgaggtacga atcgcgtgat tgatgcttca
    18061 ggggaaatcc agtctgactt ttattgctat ggaatacctg ttgaaggtgt ggattggcta
    18121 actgcagcag tagctagacc ttatacagat ccatggaatc tgagacaggc agataaaata
    18181 gcacaactta tattgactga caaataaaaa tacgcatgat gacttatctg ttttttagaa
    18241 agtcttcatg cgtattttta aataattatt aacgagaaac gcgtttacgc attgcttttt
    18301 tacggttttc ttcgatcatt gcttcttttt cgtcaattgg atcgatcact gtttttttca
    18361 cagaagcaac caaaccagca acggcagcgg ctgttgccag acttcctact aatattccag
    18421 atacaaattt tttcattgaa tccacctcat ttattatttg ttatatctat tatggaacaa
    18481 aagagaaaaa aaggctagtt ttacgtttgt ttgtaaacct ttgcaaaaaa tccttgaaaa
    18541 tgtttactaa atgttttatt ataaatttac catatgaatt tacgaaattt tactttgttt
    18601 gttttcacca taaaagtaaa cttcgttttt taaattatat agaatcaaca aacacaattt
    18661 tactaatcaa aggagtgttt tttatgacaa ttttagtact aggcggagcc ggttatatcg
    18721 gttcgcacgc tgtagaccag ctagttcaaa aggggtatca agtagctgtg gtagataatt
    18781 tgctcacagg tcacaaacaa gcagtacatc cagatgctca tttttatgaa ggagacatcc
    18841 gggataaaga atttctacgt tccgtatttg aaaaggaacc gattgaagga gtaatccatt
    18901 ttgctgcaag ctctttagta ggagaatctg tcgaaaaacc attgatgtat ttcaacaaca
    18961 acgtatacgg tatgcaaatc ttacttgaag tcatgcacga gttcaacgtg aataaaatcg
    19021 tattctcttc tactgcagca acttatggag agccgaaaga gtctccaatc acagaagata
    19081 caccagctaa tcctaaaaat ccgtatggtg aaagtaaatt gatgatggaa aaaatgatga
    19141 agtggtgtga ccaagcatat ggcatgcgct atgtcgcttt gcgctacttc aatgtagccg
    19201 gagcaaaagc agatgcatct atcggagaag atcatacacc agaaacgcat cttgtcccaa
    19261 tcattttaca agtagctctt ggtcaaagaa aagccttggc tgtttatgga gacgattatg
    19321 atacaccaga tggaacatgt atcagagatt atgttcaagt agaagatttg attgctgcac
    19381 atattttagc tttggaatac ttgaaagaag gaaatgaaag caacttcttc aatttaggca
    19441 gcagcaaagg atactcagtc aaggaaatgc tagaagcagc tagagaagtg acaggaaaag
    19501 aaattccagc agaaatagca ccaagaagag ctggagatcc tagccgatta gtcgcttcaa
    19561 gtgaaaaagc gagagagatt ctgggctgga aacctgaata cacagatatc aaagcgatta
    19621 tcaaaacagc atgggattgg catgtcagcc atccaaatgg ttatgaagaa taggggaatg
    19681 agatatgtca atcagtcaaa cgatttgtga ttttgccaca cttgcgatcg aagctggcgg
    19741 atggatggaa atggaccgct tatatttaca gaatcgtctt ttagggatgg taggagaaga
    19801 tgctctagaa agcattgaag taagaccagt taaggaagaa tctgtagact tattggatca
    19861 gcttgtcgct aaggcaagag aaaatggtgt tctttcagaa ttgccagcag aacaagagat
    19921 ttttgaagcg caattaatgg attttctcac accgccacct tcagtcgtca atgcattctt
    19981 tgcacagcac tattcgaaaa gcccagagga agcaactgag tatttcttta atttaagtaa
    20041 gaaaaatgac tacatcaaaa caagagccat cgcaaaaaac atcaaatttc cggcagagtc
    20101 ggagtatggt gaactggaaa tcacaatcaa tttatctaag ccagaaaaag atccaaaaca
    20161 aatcgcagca gaacgcgagg cggtaaaagt agactatcct acctgcatgc tttgcatgga
    20221 aaacgaagga tatagaggtc gcttgaacta tccagcccgc accaatcacc ggattattcg
    20281 aatgaatcta gacggtgaaa gctggggatt ccaatattcc ccttatgctt attacaatga
    20341 acattcgatc attttatcag aacaacaccg cccgatgaaa atcacaaagg aaacatttgc
    20401 tagactgctt cggattactg aagtcttgcc tcattacttt gttggttcga atgctgactt
    20461 accaattgtt ggaggatcga ttctttctca tgatcattat caggcaggac gtcatgaatt
    20521 tcctatggca aaagcaccga tagaaaaact tatcaaacta gaagattatc cgcaagtcat
    20581 cgcaggagtc gtgaaatggc cgatgtctgt gatccgtttg caatcaacag agaaaaactt
    20641 gcttattgaa gcagctaact cagtattgga aaaatggaaa gcatattcgg atgaaacagt
    20701 atctatcaga gctttttcag cagatggtac accacaccat acgattacgc caattgcacg
    20761 aagaagggac aattactttg agttggacct tgtccttcga gacaacaatg tttcagaaga
    20821 acatcctgat gggatttttc atccacataa agatgtccaa catatcaaaa aggagaacat
    20881 tgggttgatc gaagtaatgg ggcttgctgt tttaccacct cgtttgaaac cagaactagt
    20941 agaagtagag catttcttat taggagaaga aaaccagatg gctgaatatc atcgtccgtg
    21001 ggcagaacaa ttaaaaaaag aacatcctga tttgacgaaa gatacggtca ccgatgtcgt
    21061 acaaaaatct gtaggtcagg tttttgcacg tgtactggaa gacgctggag tattcaagcg
    21121 agatgaaaaa ggacaggcag cgtttgaacg atttactgcg attttataaa cgtaaaaaag
    21181 gaggagagac aaatggcaac aattaaagac attgcgcagt tggcaggagt ttctcctgct
    21241 actgtttcaa gagttctcaa ctatgatcct gatctttccg ttggcttagc cacaaagcaa
    21301 aaaatttttg aagcagcaga acgcttaaat tatacgaaac atcgtaaaaa tgcagcaaat
    21361 actaagcaaa cgcttcgcct gatccagtgg tacgacgata cggaagagct tgaagatctt
    21421 tattatttat ctattcgcct aggaatcgaa aaaaaggcag aagaagcaaa cgttacttta
    21481 ttgaaagaaa cgtgggattc cattagtgat cttccggcag atggaacgat cgcactagga
    21541 aaattcgacg aagctcagtt attagaaatg aaaggatccc aaaatgcctt gcttttggta
    21601 gattctgacg gcacttctca aggaatcgac tctctagttg tcgactttaa gtcaagtgtt
    21661 caaaaagtca ttgaacactt tgtctcccat aaagcgcaac atatcgctat gcttgctgga
    21721 acagaatata cgaagaaaaa ccatcagcgg ctaaaagatc ccagaattat ggcatttcaa
    21781 caagttttgg cagaaaaagg ctacgacgaa ggtccaatta tcgaagcaga tttttctgtc
    21841 gagtcaggat atcaagcagt gaagcagtat ttaaaaacga atccgcatgt tcctgatgcg
    21901 ctttttgcag ctaatgatgc attagcgatt ggcgcactga aagcgattca agaaaaaggt
    21961 ttagtggtac cagaagatat ttctgtgatg ggcttcaatg atgtcagtgt agcgaaatat
    22021 gtaacgccct cgctttctac tgtaaaagtt tataccgaat ggatgggaga gctagcagtg
    22081 gagacgattc ttcaattaag taattccaaa gcaccggttt caagaaagat tattgtggaa
    22141 acagagttga ttattcgaga atcaacgaaa taaaagatgg aaaagctgtg ggaaaatgga
    22201 gtgataccgc agcttttt
//
LOCUS       DAMEET010000046        21907 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.047, whole genome shotgun sequence.
ACCESSION   DAMEET010000046 DAMEET010000000
VERSION     DAMEET010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.047"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(304..504)
                     /locus_tag="PB780_001557"
     CDS             complement(304..504)
                     /locus_tag="PB780_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDE0733613.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(809..2041)
                     /locus_tag="PB780_001558"
     CDS             complement(809..2041)
                     /locus_tag="PB780_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDE0733614.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2295..2864
                     /locus_tag="PB780_001559"
     CDS             2295..2864
                     /locus_tag="PB780_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDE0733615.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3042..3482)
                     /locus_tag="PB780_001560"
     CDS             complement(3042..3482)
                     /locus_tag="PB780_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDE0733616.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3640..4404
                     /gene="map"
                     /locus_tag="PB780_001561"
     CDS             3640..4404
                     /gene="map"
                     /locus_tag="PB780_001561"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDE0733617.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4436..5359
                     /locus_tag="PB780_001562"
     CDS             4436..5359
                     /locus_tag="PB780_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDE0733618.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5435..6574
                     /locus_tag="PB780_001563"
     CDS             5435..6574
                     /locus_tag="PB780_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HDE0733619.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6567..7367
                     /locus_tag="PB780_001564"
     CDS             6567..7367
                     /locus_tag="PB780_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDE0733620.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7367..8194
                     /locus_tag="PB780_001565"
     CDS             7367..8194
                     /locus_tag="PB780_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDE0733621.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8172..8906
                     /locus_tag="PB780_001566"
     CDS             8172..8906
                     /locus_tag="PB780_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDE0733622.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            9006..9872
                     /gene="rfbA"
                     /locus_tag="PB780_001567"
     CDS             9006..9872
                     /gene="rfbA"
                     /locus_tag="PB780_001567"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDE0733623.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9886..10458
                     /gene="rfbC"
                     /locus_tag="PB780_001568"
     CDS             9886..10458
                     /gene="rfbC"
                     /locus_tag="PB780_001568"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDE0733624.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10480..11508
                     /gene="rfbB"
                     /locus_tag="PB780_001569"
     CDS             10480..11508
                     /gene="rfbB"
                     /locus_tag="PB780_001569"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDE0733625.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11606..12457
                     /gene="rfbD"
                     /locus_tag="PB780_001570"
     CDS             11606..12457
                     /gene="rfbD"
                     /locus_tag="PB780_001570"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDE0733626.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12492..14525
                     /locus_tag="PB780_001571"
     CDS             12492..14525
                     /locus_tag="PB780_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733627.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14569..15849
                     /locus_tag="PB780_001572"
     CDS             14569..15849
                     /locus_tag="PB780_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HDE0733628.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            16059..16865
                     /locus_tag="PB780_001573"
     CDS             16059..16865
                     /locus_tag="PB780_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDE0733629.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16877..18097
                     /locus_tag="PB780_001574"
     CDS             16877..18097
                     /locus_tag="PB780_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDE0733630.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            18087..19673
                     /locus_tag="PB780_001575"
     CDS             18087..19673
                     /locus_tag="PB780_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDE0733631.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19712..21850
                     /locus_tag="PB780_001576"
     CDS             19712..21850
                     /locus_tag="PB780_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDE0733632.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALKMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT         6910 a         3711 c         4550 g         6736 t
ORIGIN      
        1 atctattcga agggtcaaat ttaaaccata attgtgtttt tccttccatc agcataaaag
       61 ttcacctcaa aaagagtata cgaacgaatg ttctttttgt gaaggcggaa tttaatatac
      121 tgaataatag aaaacaagta cttgtgccaa attatgtgcc aaaaaaattc gaattcaaca
      181 aaatgtaaac aaatagaaaa ccttctatat taagatttcc tgcaataaca aacacaacag
      241 aagacgtgta atagttagtc aggaacgtac aaataacccc tgtatccttt gcggtacagg
      301 gggttatttt gtataagtag acattgtagt ttctaaactg ctatgtccta aacgtttcga
      361 tacgctaagt atatttactc cttgataaag taaaatagat gcatgcgtat gtcttagtcc
      421 atcaataaac agtcgaagag aaaataggga aaaattgatt actaaagaaa aacttactag
      481 gtcgacggtg tagttgattt tcatacttca atagattcga attaaacgat ttcgattggc
      541 ttataccagt cctatccaac gaaagatata tcgttcttaa ctctagcggt ataacttgaa
      601 gctttacgta aagccaatgg gaatgaactg gagatgactt tttattattt ttagtaatag
      661 ctgatgggtt aatacttaca actttccttt tcaaagtacc atataaaaaa acggtggggt
      721 tgtttagcga aataaaggat ctaagccaat agcttagatc ctttattcta tgctttatat
      781 tattttttgc atgtgtgcga aagtatactc aagcccaatc gccatttcgg aaaatcggta
      841 ctgtagagcc atcttcacgg attccgtcga tatccatctt gtcagatcct accatgaaat
      901 ctacgtgggt ttgacttcta ttcaatccag cttctgctaa ttcttcatcg gacatttctg
      961 taccgccttt cacactgaag gcataagcag aacctagtgc taagtgattc gatgcgtttt
     1021 catcaaataa tgtattgaaa aagatgatgc cagattgaga aataggtgaa gggtcaggta
     1081 caagtgcgac ttctcctaaa cgacgcgcac cttcatctgt atcaagcagt tttgctaaga
     1141 catcttctcc ttgttcggca gaaaaatcaa caacttttcc atctttaaaa gtaaatttca
     1201 tacccgagat gatggtgcca gcataactca gtggctttgt acttgaaatg taaccatcta
     1261 ctcgatgact gtcaggtgct gtgaacactt cttccgtcgg catatttgcc atgaattttt
     1321 ctccacgagc attatagctt ccagcgcctt cccaaagatg gtttttaggc aatccgataa
     1381 tgatatctgt accgggagca gtatagtgaa gggcagaaaa ttgttctcga ttcaattctt
     1441 ctgctttttt tgctaatttt tcatcatgct tcttccatgc taagacaggg tcttcttcat
     1501 agacacgagt cgttttgaaa atctgatccc ataatgcctc aacttgtttt tcttctggca
     1561 gttctgggaa gacttttgct gcccattgtt taccagccgc agccacgaca gtccaactga
     1621 ctttatttgc ttgagttgcc ttgcgtaaat tcattagtgc tttgccattc gctgattgat
     1681 aagacgctac tcgatggcta tctacaccag caaatgcatc gggatcggca gaaacaacgc
     1741 tgatcctgct tgctcctttt tcaagccatt catccgcttg atcgatttta gattgtggta
     1801 cattctcaat acgatccgtt gcagcatgta aaagaaattc tctttggatc tggtcgtcag
     1861 tccattgaac gataacctca gcggctccta gtttataagc ttcttgtgtg atcaatcgag
     1921 caaggggtgc ctgttccacg ctgatctgta aaacaactgt gtgtcctttt tctgtggcta
     1981 cgccagtttc tgcaattaag cgtgcatatt tttttagaag ttcattaaaa tcagataaca
     2041 taataaaaat tcctcctcac cattttttct ggtaactatt agcatgatac catttaatta
     2101 tgattcaaac aatgaggctt taaataaaaa aaggaggtta tttttttaag gttaagtttc
     2161 atgatatatg ttactacttg ctatttcagc ttgtgaagaa aatcaaaaac taattttgca
     2221 tatcaaaaaa acatgttaca atgaaattaa tcaatggtgg aagcgttata aaaaaattaa
     2281 ggagattaaa aaatatggca agaaaaaaga cgattactag agaacagatt ttggcagctg
     2341 cttatgaagt agctgccacc gatggattta cacgatttac tgcgagaaac atcgcgaaca
     2401 aaatgaattg ttccacgcaa ccgatttatc ttgagtttaa aaatatggat gacttgagac
     2461 aagctctttt tgagcagatc tatgatcatt tacaatatga agtgttcact gttgagcata
     2521 cagggaatac gattatcgat cttgctttta attatatcca ttttgcaaga agagagaaga
     2581 agttgtatcg ttcactgtac ttagaagaat atggtggagg aaaagagatg cagaatttct
     2641 cttatactta ctttgtgaat acagtgaaac gcgatccagc ttatgtgaat ctttcagaag
     2701 agcagatcga atctttgtat aatgggacat ggatcatagc tactgggatc gcagctttga
     2761 tgtcttcaag catcatccat ccaactgatg aacagattga aaagatgatc caagacagta
     2821 tagatgctat cttgaaattc aaagaaccaa tcgaaatcga ttgaatatac tcaaaagttc
     2881 cacaaatcag taggtaacaa gagatgtgtc aaaataagga aaagttcgtt tttcttacag
     2941 cgtatgcatt tttaactccc gaagaactct tccatgaatg caatgaataa aagagccata
     3001 cagattgaat aaatctggat ggctctttta ttgcaagtat attaaatttt tgcgactagt
     3061 tctttagcga aagcttctaa attttcgata tcttcttctt cagcattcag atccacttta
     3121 acattttcgg ctccttttga tgcgcctgtt tttgcaaaag cagcatcgaa atcatccacc
     3181 gatttgcaga attcatcgta aaaagtatca ccagatccgc agacaccgta aatttttccc
     3241 aatagatcca attcttgcaa gtcttcataa aagtctacga tctcatcagg cagatcgcca
     3301 tcatcgtatg tataagtagc tactacgcaa atatctgcct cctcgaattc atctgcatct
     3361 acttgggtac attcgttgat ttcgacttcg atatccagat tttcaaatgc ttctgcaaca
     3421 atatcggcga tttcttcagt gtttcctgtc atgctagcat atacgatttt tgctaaagcc
     3481 atgatgtgcc tcctcgttta taattattta acaattatga attataccaa aacatcattg
     3541 aaaagaaaat aaaacctaaa gacacctggg gaatgtcatt tggctaaatg tatgccgcta
     3601 tgttaaaatg agagggattt gtaaaaagga gacgaaaata tgattacttt aaaatcagca
     3661 cgtgaaattg aaatgatggc agaatctgga gctttattag cagatgtaca taaaaatctg
     3721 cgtgatttta taaaaccagg aatcacaagt tgggatattg aggtatttgt cagaaactat
     3781 atcgagagtc atggcggaat tgctgcgcaa attggctttg aagggtatga atatgcgact
     3841 tgtatcagta taaatgatga aatttgtcat ggattccctc gtaaaaagcc tttgaaaaac
     3901 ggtgatttgg taaaagtcga tatgtgtatc gatttgaaag gcggcatttc tgattcttgt
     3961 tgggcctatg tagttggaga atctacacct gagatcgacc gattgatgga agtaacgaag
     4021 aaagctttgt acatcggtat tgagcaagca caagtaggga atcggattgg tgacatcggt
     4081 catgctatcc aaacttatgt agaaggcgaa aatctagcga tcgttcgtga ttttattggt
     4141 catggcgttg gtcctacgat tcatgaagaa ccagcagttc ctcattacgg agaagctgga
     4201 aaagggttgc ggttgaaaga aggcatggtt atcacgatcg aacctatggt taataccgga
     4261 acttggaaaa tgaaaatgga tcctaacggc tggactgcgt atacaagaga cggcggactt
     4321 tcttgccaat atgaacatac attggctatt actaaagatg gtccaagaat tttgacatca
     4381 caaggagaag aaggcactta ttgatctatc cagtcaatag gtacgggaga ggtacatgaa
     4441 agtttggaac aaaataaaac aaaatgaaaa tctgatgcgc tttattgaga cgactcaaaa
     4501 gcgcatcact gattcagaaa tgagtacgac ctctgtcgtc gttgcttatt atttactctt
     4561 gtctttattt ccattgatta ttgcttttgg taatatttta ccgtttctcc acatcgatca
     4621 agagactgta ctgacctatt tacgagaagt gattcctgag accgtttacc agtttatcgg
     4681 tcctgcaatc aaagaccttt tgacacaaag ttcaggaggc ttgttatccg tttctgcatt
     4741 agcggcttta tggtctgcaa gtcaaagtat caatgctttg caaattgcga tgaataaagc
     4801 gtatggcgta gaaaatagaa aaaatttttt gatcgtccgt ttcttctcac taattgtcat
     4861 tgtcttgttc atgattgcta ttagtggggt aactatcgtt cttgggttgg gacagctgat
     4921 tttggatgct attcagccaa tcgtacaaat acccgtgaac tttatcgatc agttccaaac
     4981 tttgaagtgg ccaatcacat tggtagcttt atttgtcatc atgttcttga tttatttaat
     5041 tgtccctaat gctcagttga agttgaagac gatcattccg ggcacgatct tcgctactgt
     5101 cggctggatg ctgttatcac aagtgtttag tatctatgct aggtattttg caacgaggat
     5161 cagcggttat cagatcatag gaagttttat tgtactcatg ctttggctta attttgctgc
     5221 tacgatcatc attttgggcg gaatcatcaa tgcagttgtt caagaatacg tgacgggtca
     5281 tcaaatcaaa aaacgtcgag atccaagccg aaaatggttc aataaattga aaactaaatt
     5341 ttctcataaa aaaaagtaga aacaagagtt cttattgtaa atttatatca gtaatttata
     5401 ataattatga ctcttaatta aagtaggtgc ccatatgctt gtttctttta gtatggttat
     5461 aagatttttt ctgacaattt tattatcact gatattaaca cctgtattca aaatcatttc
     5521 agtacaaaca ggtatggtcg acaaaccaaa tgaaagaaga ataaataaag ttccaatgcc
     5581 ttcagcgggc ggactgccta tctttatttc atttgttata tcgactttat tcttattccg
     5641 taaaattatc cctcaatcgt atattgtacc gatcttgatt gcttctttag taatcatagt
     5701 cacaggagtg ctcgatgata aatatgagct gacacctaaa caaaaaagta tagggatctt
     5761 gattgcttct cttattattt attttgtagc agatatacgt attgattcct tcacgcttcc
     5821 ttttattgga tatatccaac taggctggct tagttttccc gtaacgattt tttggatctt
     5881 cggtatcacg aatgcggtca atctgattga cggtttggat gggctggcgg cagggatttc
     5941 attgatcggt ttaattacga taggaatcat tggatatttt ttcctccatg cttctacagt
     6001 gtatattcca gttgttattt tctgtcttgt agcaagtatt atcggttttt ttccttacaa
     6061 tttttatccg gcaaagattt atttaggcga tacaggagct ctattcctag ggtttatgat
     6121 ggccgtcctt tcactacaag ggctgaagaa cgtgactttt gtttcatcga tttctttgct
     6181 gatcgtcatg ggagtgccgg taacagacac gttttttgct atcattagga gaaaagcaaa
     6241 ccgtgtttct ttttctacag cagataaaaa acatcttcat caccgactaa tcagtttagg
     6301 ctttactcat aaaggtgctg tcctgacgat ttatgcgatg gctttgatgt tttctttcac
     6361 agctatggtc atgaattata ctggaagaat cggaacgatc atattgatta ttgccatgtt
     6421 gtttgctgca attttattat ttgaattgat tggtctgatc aatgagaaac atcagtttat
     6481 tttaaaaaca ttgcgatttt tagggaataa agagtaccgt acgcaagtaa aacagaaata
     6541 tttgaagaaa tggaaccgtc atatagatga ataacaaatt aaaccagccg atccttatta
     6601 gtattgtgac ggcaaacagt gataaaattt ttcggacact agataatttt atttcaactg
     6661 taaaagatga aacagcagta aaaatccgaa tttttgacaa caattcactt cccgagtaca
     6721 aaaaaagact aacagagtat aggaaatatc cctacatcga tatctatttt catgatgaaa
     6781 attccggatt tggttatgga cataatcaca atctgatagc tagcgattct cgctatggca
     6841 ttatttgtaa cccagatatt ttagtggatg aagagacagt tacagcattg gtcttgttat
     6901 tgaaagaaca tccggaatgt gcgatgttag cacctaagat tcttaatgaa gatggaacaa
     6961 ctcagcacct catccgtgag cagctggctg tttttgatta cgttttgcgc tttgtgcctt
     7021 tcaaatgggt caaaaaaaag tttgacaaac gactggctgt gtttgaatgc cgaggattgc
     7081 ctactgatgc taatagttat gtacggatga tctcaggtag ttttatggta gctgatattg
     7141 ataaatttaa agagataaag gggtttgatg agcgtttctt catgtacttt gaagacaacg
     7201 acttgtgttt gagttttgaa aaagcaggtt acaagttatt gtacacaccc ttttatgcag
     7261 ttatccattt ttacgggaag ggtgcgcatc gaaatgcaaa actgtttcgc gtgtttttga
     7321 cttctatgat gaaatttttc aataagtggg ggtggcgttt tttctgatga aaccagaaat
     7381 attagtagtc gttgtgcttt atcagcaact attcagtcaa tctccagctt atggaccatt
     7441 aagcaaagca cttagcgaaa agcaaattca gcttattatt tacgataata gtcctcaaag
     7501 gcagcaaaat gagctgtttg aaaaagaaaa tatcgtttat catcatgatc cgcaaaatcc
     7561 tggtttagcg gtggcttata attttgcgtt gtctcaagca gatgaaggaa cacgttgtct
     7621 cgtcactctg gatcaggata cgaggctagt cgacgattat tttgaactat tgcgtaaagt
     7681 aacctttaca gatgaatgtg ttgctgcagt tccgatgatt ttttctggga gccgacaagt
     7741 ttctccccta tattcagatc gttatatcaa tcgtacagct caagcagtag aagttgaagg
     7801 cccaacagct gaacgaatca tggcaatcaa ttcaggtgtt gcctggtctg tcaaatttct
     7861 aaaaaagatt ggcgggttta atcctgcttt ttctttagat tttttggatc attggctgtt
     7921 ttggaaagtg gatcagttgg aaaaaactgt ggagatcctt cctgtacgaa tggaacatga
     7981 tctttcggtt ttagactatg aaaaagtttc aagtaatcga taccgttcta ttttacaagc
     8041 agaaaatcga ttttatcatg aatatgatca aaggcattta tccagtcacc gacgacaatt
     8101 attgttaaga acagctaaac agttcttgac cgtcaaaaac cggcatatct ggcggatgac
     8161 tttgcgctca tatgttaaga attggaaggt gtgaattcgt gcgttcggtt tgtatagcta
     8221 cctttaatgg aggcagatat ttaagagagc agttagatag tattttatct cagctttcag
     8281 cagaagatga agtagtcatt tctgatgatg ggtcagtaga tgatacatgg gatatattaa
     8341 ctgaatacgc agaaaaagat aagcgaatcc agcttttttc ggggcctcgt caaggattga
     8401 tcgctaattt tggttatgcg atccagcaga cgaaaggaga tctaatcttt ttggcagacc
     8461 aagatgatgt ttggttggag acaaaagtga aaaagatcac tgactatttt gagcagaatc
     8521 cggatcagct agtagtagtt tctgatttaa tcattgtgga cagcgagctg caagagatcc
     8581 atgattcgta tttttcgttt cgaaattcca aagaaggatg gcttaataat gtgataagaa
     8641 gtcactatat tggtgctggt atggctttcc ggtcttcttt aaaacccgat attttgccga
     8701 tcccgtcaga agtgccgatg catgatatgt ggattggttt gctgggagga aagaagactg
     8761 ggttcctaag agaaccgtta actctgtacc gaagacatga tttaaacgcg agtgagattg
     8821 aaacacattc taaattacgg caaaaaattt cttggcgtgt tcatctgatt acggcgttga
     8881 tcaaacgaaa atggtttaaa cgttgataaa agatgtttaa atccttaata tttccatgac
     8941 aaatataaca attgatgaga taataacaaa gtgaaatttg aaatcaaaaa ggagagcaga
     9001 tatccatgaa gggaatcatt cttgctgggg gcagcggaac acgtctgtac cctttaacaa
     9061 aggcaacatc gaaacaattg atgccgattt atgacaaacc aatgatttat tatccaatgt
     9121 ctactctgat gttggcggga atcaaagaaa ttctgattat ttcaacgcca caagatacac
     9181 cacgtttcaa ggaattattt ggaaacggaa atgatttagg actgcagatc gaatatgcag
     9241 tacaagaaag tccagatgga ttggcacaag cgtttatcat tggagaagaa tttattggag
     9301 atgatagtgt ctgcttggta ttaggggaca atatttacta tggtggcggt ctgtctaaaa
     9361 tgttgcaacg agcagcttca aaagatacag gcgcgacagt atttggctat catgtcaatg
     9421 atccagaacg ctttggtgta gtagaatttg atgagaatat gcaagctttg tcgattgaag
     9481 aaaagcctgc tcagccaaaa tcaaattacg ctgtaactgg cctttatttt tatgacaatg
     9541 aggtaatttc tatcgctaaa ggtatcaaac cttcagaacg tggagaacta gagattacgg
     9601 atgtcaataa agcatatttg gataaaggaa aactatccgt agaacttatg ggacgcggat
     9661 ttgcttggct tgatacagga acacatgaat cattgcttga agcatctacc tttatcgaaa
     9721 caattgaaaa acgtcagaac ttaaaagtgg cttgtttgga agaaattgct taccgtatgg
     9781 gctacatcga taaggaacaa ttgctggccc ttgcacagcc attgaaaaaa aatcaatacg
     9841 gtcaatattt attgaatttg gccgctgaat aaggagtcct tacagatgaa agtaacagat
     9901 acaaaattaa cagatgtcaa aatcgtagaa atggacgtct ttggcgatca tcgcggtttc
     9961 ttcacagaaa gttattcaaa agcaaaattt gcagaacatg gattgaaata tgactttgtt
    10021 caagacaacc attcactatc agctgaagct ggtgtccttc gggggcttca tttccaaaaa
    10081 ggagaagctg cacagacaaa attgattcgg gtagtcactg gagcagtttt agatgtagta
    10141 gtggatatcc gcaaaggtag cccgacttat ggtcaatggg acggatttat cttatctgaa
    10201 cataatcatc gacagttgct tgtacctaaa ggatatgccc atggattcgt tactttgaca
    10261 ccaaatgtga acttccttta taagtgtgat aattactata atgcagaagc agatgggggc
    10321 atcgcatttg atgatccaga tttagcgatt gattggccaa ttgatccatc aagagcgatt
    10381 atgtcggaaa aagatcaccg tcatccgaca ttaaaagaat ttgaagcaga aaatccattt
    10441 gtttacggag aaatctaaga attaaggaga aattttctta tgaaaaacat cattgttacc
    10501 ggaggtgccg gattcatcgg ctctaatttc gttcattacg tggtaaacaa tcatccagaa
    10561 gtgcatgtca ctgttttgga taaactgact tatgcaggaa acaaagagaa cttggccgga
    10621 cttcctagcg atcgcgtaga attggtcgta ggagatgttg cagatgcaga attagtggac
    10681 cgtctagtaa aagaagcgga cgcagttgtt cattacgcgg cagaatccca taacgataat
    10741 tcattaaaag atcctttccc atttgtacaa acgaatatca ttggtacgta tacactttta
    10801 gaagcttgcc gcaagtatga tgtacgctat catcacgtat ctactgacga ggtttacggc
    10861 gatcttccat tacgtgaaga cctgccagga catggggaag gcgaaggaga aaaattcact
    10921 gcggaaacac catataatcc ttcaagtcca tattcttcta caaaagctgg ttcagatctg
    10981 ttagtaaaag catgggtgcg ttcttttggc ttacgtgcaa ccatttcaaa ctgttcaaac
    11041 aactatggtc cttatcagca tattgaaaaa tttattcctc gccaagtaac gagtatctta
    11101 agcggtattc gtccaaaatt atatggcgaa ggaaagaatg ttcgtgactg gatccatacg
    11161 aatgatcatt cttcagcagt gtggctgatt ttgacgaaag gcagaattgg cgaaacctat
    11221 ctgatcggtg cagacggtga agaagataat aagaccgtga tggaattgat cttagaaatg
    11281 atgggccaac caaaagacgc atacgaccat gtaaacgacc gcgcagggca cgatttgcgt
    11341 tatgcaatcg attcgacaaa attgcgagaa gagctaggtt ggcagcctga gtttacgaac
    11401 ttccgtgatg ggttggctga cacgatcaaa tggtacgaag aacatgaaga ctggtggaaa
    11461 aaagaaaaag aggccgttga agcagcatat gccaaaaatg gacaataata cagaggaaat
    11521 ttcactgtat taatggaaaa acacatgtga ggcaggcgct cgcctgtctc ttttttaaaa
    11581 taaaaatcaa ggagcggata accatatgtt tttattaact ggaggaaatg gacaattagg
    11641 tacagaattg cgccatttat tagatgaaaa aaatctgcca tatgtttcaa cagatgcaaa
    11701 agacttggat atcacagatg ctgagaaaac aatggcttat attacagaat taaaacccga
    11761 tgttattttc cattgcgcag cttacacagc agtagataaa gcagaagaag aagcaaaaga
    11821 attagatgag aaaatcaatg tcgatggcac aagaaatgta gcacttgcag caaaagctgt
    11881 gaatgcacaa ttcgtctata tcagtacaga ctatgttttc gatggaaaaa acaaaaatgt
    11941 agaatacgaa gtagacgacc aaacgaatcc actaaatgaa tatggtcgta cgaaattatt
    12001 aggcgaaaaa gcagtagaag agattttaga agactattat atcatacgaa cttcttgggt
    12061 tttcgggatc tatggacaca atttcgtctt cacgatgcaa cgcctagcgg aaacccgcga
    12121 caaactgact gtagtaaatg accagtttgg gcgaccaact tggacacgta ctttggcaga
    12181 atttatggta tatgtcatcg aagaaaaagc accatttggt atttatcatt tgtcgaacga
    12241 aaatagctgt tcttggtatg agttcgcaaa agaaatattg aaagatacag atgtagaagt
    12301 tgcacctgtt acgtcagaag aatttccgca aaaagcaaca cgtccgcagt attcagtcat
    12361 gagcctgaaa aagacagaag cgttaggttt tgtcatacca acttggcaag aagcacttgc
    12421 gcaaatgctg gaagctgcca aaaaaatgaa taagtaattt cctttttatc aaatagaatt
    12481 tggagcatga tatggaaaaa caaacaagaa gtgcacgacg aaaagaaaag ccaaaagcga
    12541 atcaggcaga ttcgaatttg gtgcttgacc tgtttctaaa actccgctat cttattggcg
    12601 ttttagtcat cgtggtcatc gttactttta atttaaacgg aagttcactt ggtgtttggg
    12661 ataaatatgt gtcgcaaaga gacgatggga aaaaaactga tgtgattttt ggacagaatc
    12721 gggaagtgcg gtcagacgaa tggctggtgc agacaccttt ttacctctca caagctgaag
    12781 aggacttttc gttggtgaac gaggattatt cattaaacgg acaaaacatg atcattgcgt
    12841 ataattctcc tgtaaaggat attacggtta tcggaaaacc attcaactgg ggattctttt
    12901 tcctcggaag agatcgagga ctttcctttt attgggcgat gaaattagtc gtcatggtct
    12961 tgcttggatt cgaagtactg atgatattaa cgaagaggaa aaaagctcta tcgctaatcg
    13021 gtgcatttgc gctgacattt tctccagcag tccagtggtg gttcatgcaa catgtgggcg
    13081 atttgatttt ctttactttg ggactgatga ttgcttttta tcattatttc tacaatcatg
    13141 ataaaaaatg gctacgaacg ttgatgatgc ttttggtggt tattttcggt attgggttta
    13201 ttttagtgat ttacccagcc catcaagtga tgctggcgta cttgctgatt ttttattttg
    13261 ttggcctgct tatttactat agcaagaaag tttcatggga tctgttggat gtagtgttga
    13321 ttggaggggc agttttattt attggcggag tcatggttca tttctggcta acgtcaaaag
    13381 acgcattgtt agcttctttg aataccttat atccaggaaa acgggtaagt actggaggaa
    13441 agtggacgat tggcgaattt ttttctttcc ttacaaattg gaaaattcct tttgaagata
    13501 tcaactattc gaataactct gaagtggccc tgttctatca cttttttcca actgtattcc
    13561 tagcaagtcc atttgtgtta tttggcaaaa aagatagcga acaaaagtta ctgggacgta
    13621 ttttgatgat tttttgtctg ttttctatct tttggattac cgttgggttg ccaaaatggc
    13681 tagcagaaat cactctgctg tcttatgttc ctccattaag agccatcctt acttttagtt
    13741 ttgcggcttg tttattatcg atctggttta tcgcttatat ttggagagaa gatgttattc
    13801 ctatttggta taaattcatt ttgttggcag taaatgcatg gtcttatttc tatgcgatta
    13861 cgcattcgaa aatggataat tatttttcgg attttgaaac catcgcagtt gctgtgttgg
    13921 gaacagtgat tttagctttt gcattatttg gctggagacg cttgttttac ctcgcttttg
    13981 tcgccttagt tatcgtatct ggctttttcg tcaatccagt cgtagaaggt acaggagcga
    14041 tttttgaaaa aacactggca aaagaaatcc aaaaaatcga tcaacaagat cctggtaaag
    14101 tttggttgtc tgaagatgaa ttatataact tcaccccaac attgggagtg aaatccttca
    14161 atacagtacg cttttatcca gatatagctg catggaagaa aatcgatccg aaagaagaat
    14221 acgagaagta ttataatcgg tatgctcata caagagcttt tatcgctaag gaagatacaa
    14281 agttcatttt agatcgtcca gatatgtttt cggtcacgtt gaattttgaa gacgcagaaa
    14341 aattagggat cgactatgtg atcagtaaac gaccattaac tgattacaac caattatatg
    14401 aagcgcagtt cacgcagcta tatggaccgg ataaagacgg ttatatgatt tttaaagtag
    14461 aatatccgga ttatccagat gtaccacaaa cacaaactca ggaaaatcag ctagtcgtac
    14521 agtaataggc tagctgagaa taatcctgaa atattagtag aaaggagagt gaagataatt
    14581 caagcaatcg tacaaaaatt tgtcaacaaa atgagtgtcg gtttcttgct tttgttgctg
    14641 acgctagtct tttttgtttg gacagcaggg gtaggttttc cagcaacttt gtttatatat
    14701 aacaatagtt cattacttat cgcttttagt gtggctattt tgctcatttt aaatgtttat
    14761 aaagcaaaag gcgtcgattt tctttatcta ggtgttgcgc ttgccctttt cattttcttt
    14821 tcttactttc gggaattaag aaatgagtct ttatttggag acttactgtt tcctttgatt
    14881 attttatttt gtttagtaat taaatggatc gagtggacaa aatgggatcg cggtatttat
    14941 ctgattgtct ttacggtctt cctcggaatc acgttatatc gtgtatttac agagatccaa
    15001 gtaccagaag gacaaagcat ctgggctcca ggcaattcgc tttctgatat ttggatcaat
    15061 acgaatacaa tcggaagttc tttgatgact cttgctttat tgatcagtgg atttgccagt
    15121 tcttttgaac gatggtatgt tcgaatcatc ggtgggatag cagtcgttgc agccttttta
    15181 ggtatttggg tctgccaatc tagaggtgca ttagtcgcgt tgattgtttt tgcgatattg
    15241 gatcttttac ctaaatcgtt gatgcgtgtc attcgtgcgc catttattgc ctacacggtt
    15301 acgattcttt tagctttgcc gatttcttat ctggcggctg tctctgaaaa agtcaatttg
    15361 tttaccggtc gagaagatat ttggcataaa ttctatcaga cgctaggaga aaaatctgaa
    15421 caagttttac taggaatgaa aacatttatc ttccagcgag gaaaccaatt tttaggaaac
    15481 cacaatagtt ataattctat tttgaatatc tatggattga taggatttgg gattgctgct
    15541 ttgctgctta tcctttttat tggtcgctta acgttgaaag cagatttatc caacgggcag
    15601 atgactttta tctgggcctt ttttgcggtg atgatgcagt catttatgga ggatacattg
    15661 acttcatacc catgggttcc gattatttat atcctgttgg ctatggccgt tcatcgatat
    15721 gatgaaccag aagttacgga ggaattctat gttatggaag aggcatatga cgatatgact
    15781 tatgatgata catcttatga ggaacaacca gatagcgctc attcttccag gatccaacgc
    15841 tatcattgat aggcacaaaa tacgagagag gctgtgacaa ccgtcgcagc cttttttggt
    15901 ttattgcaat tgtctagcat cttctgtaaa atagaaacgt tagagaaaat cttgtatcat
    15961 agaaaattaa gaaagaaata agaacgcagc tcggtaccaa aaatatggta aaatggcact
    16021 tgactgtaat tcactgaaaa tcgcttggag gaacacaaat gtttaaagat ttgtatttat
    16081 ttatgaagga catttatcag aacagaaaat tactgctaca gttttcgatc aatgacttca
    16141 aatcaagata tgcaggatcg tttttaggaa tcttatgggc gtttatcaat ccgctattta
    16201 ctgtattgat ttattggctg gtatttggtg ttggactgaa agcacaaatg atcgatgcaa
    16261 aatacccatt tatcgtatat ttagttaccg ggatgattcc ctggttcttc ttctcagatg
    16321 catttgcatc aactacggtt gtttttcggg aatatactta tttagtaaag aaagtcgtat
    16381 tcaatattcg tatcttgccc acgacgaaaa tcctatccaa tctgtacacc catttattct
    16441 tcattctgat tggcatagct gtcgcgctta taaatggtat atatccttca gcaatgtctt
    16501 tacagctgat ttattattta ttttgcatgt gtgctttctt gacaggattg acttggctga
    16561 cggcatcgat ccaaccattt ttacctgata tcatgcaatt aattacgatc gtgctgcagt
    16621 tgatcatgtg gacattaccg atattatgga gccctaatca gtttgattca ggtcttgtaa
    16681 gcatattgaa attaaatccg ctgtactatg ttgttcaagg ttatcgtgag tcatttttaa
    16741 gtgaagcttg gttctgggag catggaacat atactttgta tttctgggtt ttcacgttag
    16801 taatgttcgt aatcggctct gcagtgttcc gtagattgaa accacatttt tcagatgtgc
    16861 tgtaaggaga atagctatga cagaatatgc aattagacta aaagatatca caaaatctta
    16921 taatatgtac gcaaaacctt cggatcgatt tcgtgaagcc ctcaatcctt ttaagaaatc
    16981 ctatcatgat gtattttatg cgttgaaaaa cgtgaatgta gaaataaaaa aaggtgagat
    17041 gatcggtttc gtgggagaaa acggatcggg aaaatctaca atgttgaaaa tcattacggg
    17101 agtactgaca ccaacatccg gcacgatgga aatcgaagga aaaatctcag ctttgctaga
    17161 actaggatca ggattcaatc ctgagtattc tggctatgaa aatatctatt taaatggaat
    17221 ggttctagga ttttcaagag aagaagtcga tgaaatggtc gatgatatca ttgaatttgc
    17281 tgacatcggc gaccatattt atcaacctgt aaaaacctat tcaagtggga tgttcgttcg
    17341 tctagctttt gctgtagcga tcaatgtgaa tcctgatatt ttgattgtgg atgaagcatt
    17401 ggcagtagga gatcttgaat ttcaactaaa atgtatggaa aaatttacag aaatcaagaa
    17461 ctcgggaaag acgattttat tcgtttctca tgatattagt tcgattcgcc gtttttgtga
    17521 tcgttcctat tggttgaaaa atggaaaagt tgtcgaatat ggtgatacga tggaagtaac
    17581 gacgaattac gaaaactttt tgaagaaaaa atcagttaaa acagtagatc gaaataaaaa
    17641 ccaagtagaa cgcttcgcag ctccagatat cgtagacgtg acgaaagccg aattgttgga
    17701 taaagataaa aatccagtgg atatgctgga acaaggagaa aaactatacg ttaaagtaac
    17761 ctatgaagta aaagacgata caatcaaaaa tccagtttta ggaattgctc tgcgtacagt
    17821 cgacaatttt tatgtgtgcg gactcaatac tctactagat ggtatcaaga tcccttggaa
    17881 aaaaggtgag aatatctttt atctggagta tgaaaagtta ggtttgttag ctggtgaata
    17941 ttattttgac gttgcggtct ttgaagaaaa tgcaacggtt cctttggtat acaagacaaa
    18001 atacatgaac ttgtttgtca gcggttctta tatcggagaa ggcatcgtgg tcttagacca
    18061 taaatgggaa gaagggactc atagcaatga aatatgattt tgaaatggac ctagatgaac
    18121 aaagcagtgt gggaaaaatt gccgcacaga tcaaaccagg aagtaaagtt ttagagtttg
    18181 gtcctggaaa cggccgatta acgaaacatt tgatcggagc aaaacaatgt gaagtgagca
    18241 ttgtcgagct agataaggaa ttgttcgatt ttgtcagcga gtttgcccaa gatggatttt
    18301 atggagatat cgaaagtttt gaatgggcaa actattatgc tggacaaaca tttgattacg
    18361 ttttgtttgc cgatgtttta gaacatttgg tagatcctgg aaaaacgttg aagaaagtta
    18421 gagaattttt gaatgaagaa ggagaaattc taattacatt ccctaacctt gcgcataatt
    18481 ctgtcatgat tgatttgttc aataatcaat tgccatgggc ttcttatggt ttgctagatg
    18541 aaacccataa ttctttttat actcatgatg gcttccaaaa agtttttgaa aaagcaggat
    18601 tatacatcaa tattgaagat tatttgtact tagctgtagg tgatacagaa ttaaaatcaa
    18661 cttatgaaga attgccggaa gctgtacgtt atgatttcaa aatgcgacca tttggcgaag
    18721 tctaccaata cttcttctca ttgatgaaac atccagtagc acaaagttcc attgcgcagc
    18781 cgcaaaattc taattatgtg aaagtattag aagtaatcca gcaaacgaaa caagatgaaa
    18841 taattcaaca aataccattc aataatttca caggggaaaa tgagacacta agtttccatg
    18901 tatcagaatt agcagaacga atggtgtttc gcttcgcgca gcaacctagc tttatcgaat
    18961 tcagcgcaga agctgctgga gaaaaactca catttatcga ttcaaatgct gtagtcaaaa
    19021 cagtaaatga ttgttatttg tttgatggta aagatttacc agagttcttt ttaacggata
    19081 ttgcagggaa agaagtgact atccattgtc actatcgttt tatcggagaa ttgacaccaa
    19141 caatgaaaga attgctggaa acaatccgtc caatggctga agttacaaag cagctttctg
    19201 agaaaaatga tgaattagaa agagaaaatc attatcttat tgagaataat acaagattag
    19261 atcagacgtt gaaaacaacc ataaatcgct attgcacgtt gttggaaagt gatgaatgga
    19321 caatcaaaca tagattaggg cgacgtaaga aagaaacatc gaaaaaaatc caagaaaaag
    19381 agctttctct atgtattgac gaaaaaatct gggatgcaga aacaaagata ttgaaaatca
    19441 tcgggtgggg aatcgctaat tcggatcgcc aaccattgtc ctataagtta tcagccgacc
    19501 aatcaccatt ttttgaagca attcctgttt tacgagaaga agtcaatcaa gcaaaacagc
    19561 tagctgaagg agcaaaagca ggatttgaac tgcggatcct ttgcgagaag gaacgttcat
    19621 tcttagttga agcagtggct caaaatggtc aatcatggat cattgaaatg taaataatga
    19681 ttaacttata aagataagga gtaaacgaat cgtgcaaaaa aaagaaattg ccgtatttat
    19741 cgatcaaatc actcgtgaac gggccactgg cgatttgctt attgtgggct gggcaattga
    19801 tgaagtgacc aaagaaatcc ctacgataaa ggtagaaaaa gaaaatgtga ttgctgaagc
    19861 aacacatgtc gtaagattgg atatcaatca tttatataat cttgatgtta agacccaaag
    19921 cggttttaag gtacgtttat cagggaaaat gagaggaaaa gcaatcttag acttccaaac
    19981 agcaagacat caaaatggca tagctgtgaa attaaacggt aaatatcctt atgatgacgg
    20041 cattgaatct tcatgggaaa gaaaaaaaag attactgaaa aaagggataa attatgcaag
    20101 gactcatggc gtaaaaaaag caatccgccg ggtcaaactg gaattgaaac caggatcgat
    20161 cgactatgca gagtggatca gtcgtcatga gaaacctgat ctgaaagagc aaaggaaaga
    20221 agtacaagga tttgcctacc gcccattgat ttccatactt atgcctgtct ataacgttga
    20281 aatcaaatgg ctcgaaaaat gcattgattc ggtacttgat caaacgtatg atcactggga
    20341 actatgtatc agtgatgatg cttctactga tccagctatc cgaaagtgtc tagaatccta
    20401 tcaagcaaaa gatgatagaa ttaaagtcgt tttccgacaa gaaaatggac acatcagtct
    20461 ggcaactaat tctgcattga aaatggcaga aggagagttt atagcgctct tagataatga
    20521 tgacgaatta cctccatttg ccttatatga agtggcaaaa gtgctaaacg tacatccaga
    20581 actggatttg atctacagtg acgaagataa aatcgatgca gatgggaatc gctttgaccc
    20641 gcacttcaaa gcagactggt ctcccgatac attgatggga aataattata tctctcactt
    20701 aggcgtatat cggacaagca ttgttaagga acttggcggt ttccgtaaag gatatgaagg
    20761 ctctcaagat tatgatttgg ttcttcgtgt gacagaacaa attcctgcag aacacattta
    20821 ccacattgac cgtgtcttgt atcattggcg gacgatcccg ggatcaactg caagcaatgg
    20881 agaagctaaa agctacatct atgattctgg agtgaaagca ttgacagatg ctctatctag
    20941 aagaaatatc aagggaagcg tacatcctgg cagaatttct ggtttttatg aaatcactta
    21001 tgatgttttg caagaagact tggtcagtgt gatcattcca actaagaatg gctatgaaga
    21061 tctaaaaact tgtgtggatt cgatcatcga aaaaacgtct tatccaaatt atgaaatcat
    21121 tattgcagat aatggaagta cagatcctaa aatgcaagaa ttatttgcag aatacaagca
    21181 tcagctgaaa gaccgattta tcgtcgaatt aattgacatt ccattcaatt attcacgtat
    21241 caacaatctg gcagcagaaa aagcaaacgg caaatacttt ttattcttga ataacgatac
    21301 agaagtgatt gaacctgact ggatgacagc gatggtttct tatgcacagt ttgatcgaat
    21361 cggctgtgta ggagcaaaat tattttatcc ggatgataca acgcagcatg caggtgtatt
    21421 attagggatc ggtggcgttg caggacatgc attgaataac tatgatcgaa cacattgtgg
    21481 ttactttggc cgattggtca ttgacgtcaa ttacttagca gtcactgcag cttgtatgat
    21541 ggtcaaggca gctgacttca atgctgtcaa tggatttgat gaaatattag aagtagcatt
    21601 caacgatgtt gacttgtgct tgaaagttta tgaactgggt cgttacaatg tctatgcaca
    21661 tcaagcagaa ttgtaccatt tcgaatctaa atcaagaggt tatgaagata caccagaaaa
    21721 acaaagacgt tttgctgggg aaatcaaaaa aatgcaagat aagtggcctt cttatattgc
    21781 tcatgaccca ttttataacg ataatctaac taaagaagga atcggcgatt tttcgttgag
    21841 acctgattga ttaaaagaac tgtattttca tttttattaa tgaaaataca gttcttttaa
    21901 tttaatt
//
LOCUS       DAMEET010000047        21594 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.048, whole genome shotgun sequence.
ACCESSION   DAMEET010000047 DAMEET010000000
VERSION     DAMEET010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.048"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            177..404
                     /locus_tag="PB780_001577"
     CDS             177..404
                     /locus_tag="PB780_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDE0733633.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            420..1586
                     /locus_tag="PB780_001578"
     CDS             420..1586
                     /locus_tag="PB780_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDE0733634.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            1821..2864
                     /gene="hrcA"
                     /locus_tag="PB780_001579"
     CDS             1821..2864
                     /gene="hrcA"
                     /locus_tag="PB780_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HDE0733635.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            2958..3521
                     /gene="grpE"
                     /locus_tag="PB780_001580"
     CDS             2958..3521
                     /gene="grpE"
                     /locus_tag="PB780_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDE0733636.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDRYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            3572..5401
                     /gene="dnaK"
                     /locus_tag="PB780_001581"
     CDS             3572..5401
                     /gene="dnaK"
                     /locus_tag="PB780_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDE0733637.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            5552..6718
                     /gene="dnaJ"
                     /locus_tag="PB780_001582"
     CDS             5552..6718
                     /gene="dnaJ"
                     /locus_tag="PB780_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDE0733638.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(7086..7823)
                     /locus_tag="PB780_001583"
     CDS             complement(7086..7823)
                     /locus_tag="PB780_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDE0733639.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(7825..9327)
                     /locus_tag="PB780_001584"
     CDS             complement(7825..9327)
                     /locus_tag="PB780_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HDE0733640.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIEKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            9586..11580
                     /gene="uvrB"
                     /locus_tag="PB780_001585"
     CDS             9586..11580
                     /gene="uvrB"
                     /locus_tag="PB780_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDE0733641.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            11590..14406
                     /gene="uvrA"
                     /locus_tag="PB780_001586"
     CDS             11590..14406
                     /gene="uvrA"
                     /locus_tag="PB780_001586"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDE0733642.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            14669..15934
                     /locus_tag="PB780_001587"
     CDS             14669..15934
                     /locus_tag="PB780_001587"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HDE0733643.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            15938..16669
                     /locus_tag="PB780_001588"
     CDS             15938..16669
                     /locus_tag="PB780_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HDE0733644.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            16804..17688
                     /gene="rapZ"
                     /locus_tag="PB780_001589"
     CDS             16804..17688
                     /gene="rapZ"
                     /locus_tag="PB780_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDE0733645.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            17685..18683
                     /locus_tag="PB780_001590"
     CDS             17685..18683
                     /locus_tag="PB780_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDE0733646.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            18717..19652
                     /gene="whiA"
                     /locus_tag="PB780_001591"
     CDS             18717..19652
                     /gene="whiA"
                     /locus_tag="PB780_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDE0733647.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            20195..20845
                     /locus_tag="PB780_001592"
     CDS             20195..20845
                     /locus_tag="PB780_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733648.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            21032..>21594
                     /locus_tag="PB780_001593"
     CDS             21032..>21594
                     /locus_tag="PB780_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDE0733649.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT         7103 a         3658 c         4712 g         6121 t
ORIGIN      
        1 ttttttatga ataaaataag gacaaaattg tttaggaaga atcatgagtg taaaaaaatc
       61 atccaagaag tcgattcttg gattttttag tggatttgtt cgccaaatac gtttagtatg
      121 cgtatatgag aggttgtaag caaagcgttg tggtctgagc acttaaggag gaatctgtga
      181 aaatagcttt tcatattttt gcagattttg acttaatgcc gaaggtcagc tttgcgatta
      241 ccgtttatcg agaggctgta aaagcaccgt gtagctccga tgacaataag caagcatttt
      301 tagagctagg tttccttgat tctaaatatg cggcttattt caatgagttg gtgcttgaac
      361 gcgattttaa gaaggtcgaa aacaggcacg acttttcgac gtaaggagaa agaatttcaa
      421 tgatagaaac aataaatccc gcagaaaaca aggtttctgc gtacattcat atcccatttt
      481 gcgagcatat ctgctattac tgtgacttta ataaagtttt tttagaaggg cagccagtag
      541 atgagtatgt ggaaatgctg ctgaatgaaa tgagattaat gctggaaagt catcctacag
      601 cgaatttgga aacactttat gtaggaggcg gaacaccaac ttctttatct gctaaacaac
      661 ttgatcgact attatcaggt gctagagaga tcctgccttt tcaaaatggt aatgagttta
      721 ctgtagaagc gaatcctggt gatttgaccc gtgaaaaact tcaagtgatg aaaaactatg
      781 gcgtcaatcg tttatccatg ggagtgcaga catttgacaa tcgcttattg aaaaaaattg
      841 gacgtaaaca tactgcagaa gatgtgtatc aaacaatgca atttttagaa gcagaaaatt
      901 ttaccaatgt aagcatcgat ttgatttatg cattaccggg acaaacacta gaaggatatc
      961 gggatacttt ggaaaaagct ttggctttgg atctgcctca ctattctttg tactcattga
     1021 tcctcgaaaa caaaacaatg ttcatgaact gggtacgaca aggaagactc gaactgcctg
     1081 atcaggaaac ggaaaccaga atgtttgaag agacgattga agcaatggaa aagcatggac
     1141 gtcaccaata tgaaataagt aactttgggc tctccggaca tgaatctcaa cataacctca
     1201 tgtactggaa caatgaccac tattttgggt ttggagctgg tgctagcggt tatcttggag
     1261 atattcgtta ccgcaacaaa ggaccgatcc aacactattt acgcccatta cgagcaaatg
     1321 aacgcccggt attggaagaa gaaaaattga cacgaaaaaa tcaaatagaa gaagaaatgt
     1381 tcttaggact aagaaaaaaa acagggatta ctaaaaaaca tttctatgat cgctatcaaa
     1441 aaacaattga atcggtgtac ggtgatgtgc taagggaatt agagcaagaa gggttacttg
     1501 ttaatgaaga agaccgtgtt tatttgacac caagagggac ttttttgggg aacaatgtgt
     1561 ttgagcgctt tttatttgaa aaatagtttg aagaatattt gttttttatc gaaacttgaa
     1621 aagaaaaagt ttagaaagtt tgacaatgag gtattgaagt attgatataa ctggatttac
     1681 atcaattatt tagaattagc actcttgtgt ttagagtgct aaaaaatgga gtctttctct
     1741 tgacaggaga agggcttctt gctatattta taagtgaatt agcacttaga atatatgagt
     1801 gctaatgaga ggtgaggaag atgttaacaa agcgacaaaa cgacatttta cgtctgatca
     1861 tccaacacta tacgagtagt ggcgtgcccg ttggttccaa aacattaatg tctgagggag
     1921 tcaaggccag ctcagcaacg attcgcaatg atatgaaagc attagaagat gaaggattgc
     1981 tgttgaagac tcactcttct tctgggcgga ttccatctgc tttaggctat cgttattacg
     2041 tggatcattt gttaaaacct gctcgcgtag cagaagatga tttgctgctg attcgtcagt
     2101 ctctaggcag agaatttcat gaaatcaatg agattattaa gcaatctgct gagatattat
     2161 ctgagctgac aagctatacg actttttcat tgggacctga aataaaagac cgccgactga
     2221 ctggctttcg tatcgtaccc ttgaataatc gacagattat tgctatcgtc gtaacagata
     2281 tggggaatgt agaaagccaa gtgttcactc ttcccgaata tatgggaagt caggaactag
     2341 aaaaaatggt acagattgta aacgaccgtc tagtcggaga tcctctgatg acggtgtacc
     2401 ataaacttcg tacggagatt ccgatgattc ttcataaata tttccagacg acagaaggga
     2461 tgtctcagtt atttgacacc gttcttggtc atgtttttga agaaaaggtt tatgtcagtg
     2521 gacaaatgaa tctcttggat tatgagcccc gtcaagatct tgatcagttc aaatcgatgt
     2581 tttcttttat gaaagactca gacggactta cacaaatgat cactccgatg gatagcaata
     2641 ttcatatcaa gattggtgct gaattaggca atgatttatt gcagaatatg agtatgattc
     2701 aagcaagtta cgagatagat ggtcatggaa gaggtacaat agccttgtta gggcctacta
     2761 gtatgcctta ttctcggatg ttcggtctag tcgatgttta tcgaagagaa ctagcagcta
     2821 aacttgctga ttattaccgc tcgcttgact tgtcaaactc gtaatgtgaa taacgaagct
     2881 atttgcagct gatcgttctc tctttcttag taagagcaat caaacagctg actgaataat
     2941 gaaaggaaga ctgtatcgtg aaagaaaatc aagaagaact agataaagaa atgacagatg
     3001 ctcagccaga accggaaatc gatgtcgaag ctgcagaaga ttcgggtatt tcagaagctg
     3061 aagcggaaga attcgaaaca gcgaaattaa aagctgagtt agaagaaatg gaagatagat
     3121 acttgcgggc tcgtgctgaa atcgcaaata tggcaaatcg aggaaaaaat gagcgtgagc
     3181 aattacaaaa ataccgttca caagatttag ccaaaaaatt acttccatca attgacaacc
     3241 tggaacgtgc attagccaca gaagtgtcag atgatcaagg ggcaggtttg aaaaaaggcg
     3301 tggaaatggt cttggaaagt ttgcgtaacg cactggaaga agaaggaatt gaaaaaatcc
     3361 ctgctaaagg agaagccttt gatcctaatc ttcatcaagc agtacaaact gttccagcga
     3421 ctgaagatac accagccgat acgattgtag aagtactaca agaaggctac aaattacatg
     3481 atcgcgtgtt aagaccaact atggtcattg ttgcacaata attgtgcgga tataaaactt
     3541 acactttcaa ttaaagaagg agacgttaat tatgagtaaa attatcggta ttgacttagg
     3601 tacgactaac tctgcagtag cagtattgga aggaaacgaa gcaaaaatca tcgctaaccc
     3661 agaaggaaac cgtacaacac cttctgttgt atcattcaaa aatggagaaa tccaagtagg
     3721 ggaagttgca aaacgtcaag ctgtgacaaa cccaaatacg atttcatcaa tcaaacgcca
     3781 tatcggtgaa gcaggttaca aagtagatgt agaaggtaaa tcatatacac cacaagaaat
     3841 ctcagcaatg atccttcaat atatcaaagg attcgcagaa gactatttag gtgaaaaagt
     3901 cgaaaaagca gttatcactg tgccagctta ctttaatgat gcacaacgtc aagcaacaaa
     3961 agatgctggt aagatcgctg gtctagaagt agaacgtatc gtcaatgaac caactgcagc
     4021 tgctcttgcc tatggtttag ataaaacaga tcgcgacgaa aaaatcttag tattcgacct
     4081 tggtggcggt acttttgacg tatctatcct tgaactagga gacggtgtct ttgatgtatt
     4141 gtcaacagct ggcgacaacc atctaggtgg ggacgacttt gataacaaaa tcatcgacta
     4201 catggtagca gaattcaaaa aagaaaacag cattgacttg tctcaagaca aaatggcttt
     4261 acaacgcttg aaagacgctg ctgaaaaagc gaaaaaagat ttatctggcg tatcaagcac
     4321 acaaatcagc ttgccattta tcacagcagg agaatctgga ccattgcatt tggaaatgac
     4381 attgacacgt gcaaaatttg atgaattaac agctgactta gtagaacgta caaaagtacc
     4441 agtacgtcaa gcgttaaaag atgcaggtct gtcacaatca gaaatcgacg aagttatttt
     4501 agtcggtgga tctactcgta tccctgcagt tgtagaagca gtacgtaaag aaacaggaaa
     4561 agaaccaaac aaatcagtta accctgatga agtagttgct atgggtgctg ctatccaagg
     4621 tggcgtgatc actggtgatg tgaaagatgt tgttttactt gacgttacac cattgtcatt
     4681 aggtatcgaa acaatgggtg gagtgttcac taaattgatt gaccgcaata caacgattcc
     4741 aacaagtaaa tcacaagtat tctcaactgc ggcagataat caaccagcag tagacatcca
     4801 tgtattacaa ggtgaacgcc caatggctgc tgacaacaaa actctaggaa gattccaatt
     4861 gacagatatt ccagcagctc ctcgtggtat tccacaaatc gaagtaacat tcgatattga
     4921 taaaaacggg attgtaaatg tatctgcgaa agatctaggt acacaaaaag aacaaaaaat
     4981 cacgatcaaa tcttcttcag gtctaacaga tgaagaaatc gaacgtatgg tgaaagatgc
     5041 agaagcaaac gctgaagcag ataaagctcg taaagaggaa gttgacctac gtaatgatgt
     5101 agacgcattg ctattctctg tggacaaaac attgaaagaa ttagaaggca aagtggacga
     5161 agaagaagtg aaaaaagcag aaacagctcg tgacgaatta aaagccgctg ttgaagcaaa
     5221 caacatcgag gaaatgaaaa ctaaacgcga tgcattgaac gaaatcgttc aaaacttgac
     5281 tgttaaacta tacgaacaag ctgcacaaca acaagctcaa gaaaatccag aagcagctca
     5341 aggcggagca gatgatgtcg ttgatgctga ttttgaagaa gtcgatggcg acgataaata
     5401 atcagttcac tgaatttcga gagaaggcca aagttgctac tttggctttt tcttttgaaa
     5461 aatagtttga agtatcggtt tttgtttcaa acaaaatatg atatgataac aagcgacgtg
     5521 aaaatgatga gaaccaatag gaggaaagcc tatggcaaca aaacgtgatt attatgaagt
     5581 cttagggctc tcaaaaggag catcagaaga tgaaataaag aaagcttatc gtaagctttc
     5641 gaaaaaatac catcctgaca tcaataaaga acctgatgcg gaagaaaaat tcaaagaagt
     5701 atcagaagca tatgagattt taagtgaccc gcaaaaacgc gctgcatacg accaatacgg
     5761 gcatgcaggc acagatccaa attacggtgc tggtggagct ggtggcggat ttggtggatt
     5821 cggcggcttc tctggtggcg gcttcggtgg ttttgaagat atcttcgact ctttctttgg
     5881 aggaggcggt cgatcagtcg atccaaatgc gcctcgtcaa ggtgctgatt tgcaatatac
     5941 ggtcgacttg tcttttgaag aagcgatttt tggtgtagaa aaagaaatca agtataatcg
     6001 ggaagaaatc tgtcatactt gtggtggaaa cggtgcaaaa ccaggcacac aaccaactac
     6061 ttgtcataaa tgtcatggtg caggtacgat caatgtagaa cgccaaacgc cacttggacg
     6121 agtgatgagc cgtcaaactt gtgatgtttg tcatggcaca gggaaagaaa tcaaagaacc
     6181 ttgtccaacc tgtcatggct caggacatga gaagaaagca cattcagtta aagtgaatgt
     6241 accagctggt gtagaagaag gacaacaaat gcgtttagct ggtcaaggag aagctggaga
     6301 aaacggcgga ccatttggtg atttgtatgt ggtctttcgt gtagaagaaa gtgatatctt
     6361 tgatcgtgaa ggttctgaaa tttactatga attgccttta agttttgtac aagcagcttt
     6421 gggggatgaa gtccaagtac caactgtcca tggagatgtg aaattgaaga tccctgctgg
     6481 aacacagaca ggtacgaact tccgtttacg tggaaaaggc gctccgaagt tacgtggtgg
     6541 tgcaacaggc gaccaacatg tcaaagtaaa actgatcaca ccgaaaaatc taaatgaaca
     6601 acaaagagaa gcattacgtg cattcgctga agctggcggc atcaaagttg aagaacagca
     6661 agaagacggt ttcttcgata agtttaaaga tgcttttggc gggaaaaagc ggaagtagtt
     6721 tttctagtaa aaatataaag tagacttgcc caaattttct atttctagta aaaacgctaa
     6781 gtcattaata attaaaaacc agacgaacaa tcatacgttc gcctggtttt tttgtagttg
     6841 tcatgtttgt accaacaaaa aagccagctt tagaataaga taatcatcat agtttctgaa
     6901 ggtggtatcg actatgtact tgatatcgtc aatgaaccga tggtactttc ttatgcgatg
     6961 gaacaggtag agcgattgct tgtttgtgca ggagaacaag cgatgcggct ggtacagtta
     7021 agtgaaaaaa caagaaacta aaaatgcccg gtcgtcatga agcaaccggg catttttggt
     7081 ttttattaaa tattcaatac tttattcaag aaatcttttg tccgttcatt ttgcggattt
     7141 tcaaagattt gttgaggcgt tccgtcttct aaaaagtttc cgtcgtcaat gaacatgaca
     7201 cgattggcca cttctttagc gaagcccatt tcgtgcgtaa cgataaccat cgtcatacct
     7261 tgttctgcta actttttcat gacgttcaag acatcaccga ccatttcggg gtcgagtgca
     7321 gatgtaggct catcaaaaag catgatgtca gggttcatcg ctagagcacg agcaatagcc
     7381 acacgctgtt tttgtccacc agaaagcatt tcaggataca tatctttttt atcttctaga
     7441 ccaactgtct gcagtaattt gattgctttt ttctctgctt cttctctaga ttctttcttc
     7501 aggtccatcg gtgctaaaat gatgttttcc aaaatagaaa gatgagggaa taggttaaag
     7561 tgctggaata ccattccgat atgttgtctc accaagttga tatctgtgct tttatccatc
     7621 aagtgggcgc cgtcaataat aatttcaccg tcagaaggtt cttctaaacg attcaagcaa
     7681 cgcaaaaatg tactttttcc tgaacctgaa gggccaatga tacagacgac atctccttcg
     7741 ttgatggata cattgatgtc gtttaatact gtgttatcgc cgtatttttt gactaaatgc
     7801 tcaactaaga ttttttctgc catcttactt caccttcttt tctaaaactt ttgctaattt
     7861 tgtcaatgct gtgatcaaaa tcaagtacat gatcgcaatg atcaagtaaa catacgtact
     7921 ttgtgttgta cgtgcaacaa tgatcttacc agtttgcagc aattcgacta caccgattgc
     7981 ggaaatgatc gttgtgtctt ttaatgagat aacgaactga ttgacgaatg atgggatcat
     8041 gatgcgtatt gcttgtggca aaatgatttt ttgcattgtc cgattataag aaagacctaa
     8101 acttctggaa gcttccattt gtccgacagg aacggcgttg atcccgcctc gaacaatctc
     8161 tgcgatataa gcacttgcgt taagagtcaa ggtgataaca ccagcaagga agtcaggaat
     8221 cgtaaatcca acgataccag gcaacccaaa gaaaataaag aacgcaagaa ccatcattgg
     8281 aattcctcgg atgatatcga cataaatact agcaattgtg cgaagagcgc gtattggtgc
     8341 tacactgaat aatccaaaaa tcacaccgat tactatagcc aaagcaaagg agatcaaagt
     8401 taggagaatt gttctccaaa gtcctttcaa caatgcttga tagttgtttt ggataagtcc
     8461 aagaaatgtt gattcatcag cagtggaaga ctcactagaa gtttctgaac catccgctac
     8521 atattctttg ataatcttat cgtattcgcc tgttcttttt aattctttca atccttcatt
     8581 gaacatatta agaagctcag gattttgtcc ttttttaacg gcaaatgcat attttccgcc
     8641 agattctttt tcgatcggtg tttccaattc ttgtccttgt ttgatcgcat aaccgattac
     8701 cggataatca tccatcattg catcgattgc gccaactttt aacccgtcgt acaattgatc
     8761 agcagcatca aaatatttga tagaatagcc atattcagat tcatggtcgc ttaaaaattc
     8821 agcactttcg gtaccaactt ttgctccgac tgtttttcct ttcaaatctt cataagactt
     8881 gatcgaagta tctccttttt tgacagctaa ttggatcccg ctatcaaagt aagaatcaga
     8941 aaaatcatag cttttctttc gttcatctgt tacagtcatt ccggcaatca ttccgtctgc
     9001 ttgtcctgat tccaaagctt gcaaagcagc agagaaacca cggtagtcca ttcggatgtt
     9061 aaatccttgc agtttggcga tgttttccat caattttaca tcgatgcctt cgtactcgcc
     9121 ctttgcgttt tgaaattcaa atggagcaaa agcagagtca ctggcaatta cgtatacgtc
     9181 ttttttgggc tctatttttt gcattgtctc gcttgtgctt gactctgccg ttgtagtgtt
     9241 gtcagcttgt gcaacatgac caaacgcaag tgtcacaagt cctgtcatta agaaaacaaa
     9301 tgagtgaaat aaatgctttc ttttcataat caacctccta aatttccaat attttattat
     9361 tataggcgaa aagtgctgaa tcagacaaga ctgtaagctc gaaaaaatga aaaaaatgcg
     9421 agtttctaac attttatgtt ataaaacgta acatgtataa taagataaaa aagtggcgaa
     9481 agtatgttcg gtgtggtata attttttagt aaggaatgcc tttgctttcc ttttcttttt
     9541 attggtcgta aaatgaaaag gaatgaatga aaaggaggac gaagaatgat tgagagagat
     9601 acctcccgac actttgagct tgtctcgaaa taccaaccgg ctggtgatca gcctgaagcg
     9661 attaaccaat tagtggatgg cgtagtcggc gggaaaaaag cacaaatctt attaggtgcg
     9721 actggaacag ggaaaacata tacgatttcc aatcttattg agaaagtgaa taaaccaaca
     9781 ctgattattg ctcataataa aacattggct ggtcagctgt acggggaatt caaggaattt
     9841 ttccctaaca atgctgttga atattttgtt tcttattatg attattatca gcctgaagct
     9901 tatgtgcctt caagcgatac gtatatcgaa aaagattcaa gtgtaaatga tgagatcgac
     9961 aaattacgac attcggctac tagttcatta ttagaaagaa atgatgtgat cgtcattgca
    10021 tccgtttcat gtatttttgg attaggttcg ccttttgagt accaaaaaca agtagtttcg
    10081 attcgacagg gagcagaact agatcgcaat cagttgattc gtgacttagt cagtatccaa
    10141 ttcgaaagaa atgatataga ttttcagcgc ggtcgttttc gagtgcgcgg tgatgtcgtg
    10201 gagatttttc cggcttcaag agatgaacgt gccttacgtg tggagttttt tggagatgag
    10261 gtcgaacgta tccgagaagt caatgcactg acaggtgaag ttttaggaga aacggaacac
    10321 gtggcaatct tcccggcgac tcactttgtg acgaatgatg aacatatgga gcatgccgta
    10381 gcgaatatca aagcggaatt agaacagcgt ttaactgtct tgcgaaatga aaacaaactt
    10441 ctggaagctc aacgtttaga acagcgaacc aattacgata ttgaaatgat gttagaaatg
    10501 ggctacacgt cgggaattga aaattattct cggcatatgg acggacgtaa agaaggtgaa
    10561 ccgccttata cgctattgga tttctttcct gaagattttt tgatcgtggc ggacgaatcg
    10621 cacgtaacca tgcctcaaat cagaggaatg tataatggtg accgagcgag aaaacagatg
    10681 ctagtagatt acggtttccg tttgccatct gctttagaca atcggccact tcggttagaa
    10741 gagtttgaaa aacatgtcaa tcagatcatt tatgtctctg cgacgccagg accatatgaa
    10801 catgaacaaa cagataccgt tatccaacaa atcatccgtc cgacagggct gcttgaccca
    10861 gtgatcgaag ttcgtccaat catgggacaa atcgatgatc tagtaggcga aataaatgaa
    10921 cgagtcgaaa aagaccagcg ggtgtttgta acgacattga cgaaaaaaat ggcagaagac
    10981 ctcacagatt atttcaaaga attagggatc aaagtcaaat atttgcatag tgatatcaaa
    11041 acgctggaac gaacagaaat cattcgagat cttcgattag gtgaatttga tgtgttagtt
    11101 ggaatcaatt tgcttcgtga aggattagac gtacctgaag tttcccttgt tgctatcttg
    11161 gatgcggata aagaaggctt tttacgtagt gaacgttcct tagtccaaac gattggacga
    11221 gctgctcgga atgaagaagg taaagtaatc atgtacgcag acaaagtaac agattctatg
    11281 cgtctagcga tggatgaaac gtcaagacgt cgtacgattc agcagaagta caatgaagaa
    11341 catgggatcg ttccaaaaac gatcattaaa gaaatccgag atttgatttc tattacgaaa
    11401 gaatcggaag atgatacaaa agaagcggta caagtctcgt acgaagaaat gacgaaggaa
    11461 gaaaaagaca cgttattgat gaaattagaa aaagaaatga aagatgcggc aaaagctttg
    11521 gattttgaaa cagccgcgaa tgtgcgagat atgattttag aattaaaagc ttcaaagtag
    11581 gaggacaaca tggctaacga taaaattacg attcatggtg cacgtgccca taacttgaaa
    11641 aatatagatg taacgattcc acgagataaa ttcgtggtcg ttacaggttt gtccggttct
    11701 ggaaaaagtt ccctagcatt tgatacgctg tacgctgaag ggcagcggag atacgtagag
    11761 agcctttctg cttatgctcg tcagttttta ggacaaatgg ataagccgga cgtagatagc
    11821 atcgacggat tgagtccggc gatttctatt gatcagaaaa caacaagtaa aaatcctcgg
    11881 tcgacagtag gtactgtgac ggaaatcaat gactatttac gcttgttgtt tgcacgtgta
    11941 ggacatccga tttgtcccaa cgatcatatc gagattacca gtcaatcagt agatcaaatg
    12001 gcagatcaag taatggcttt gcctgagcgc acaaagatcc aagtgttggc accgatcgtg
    12061 gtaaagaaaa aaggacaaca taaaaaaata ttcgaacgaa tccaaaaaga gggatacgtc
    12121 cgcgttcgag tcgatggtga gatctatgat ttaagtgaag tgcctgagct ggagaaaaac
    12181 aaaaaacatg atatcgcgat cgtcatcgac cggatcattg tgaaagaagg gattcgttct
    12241 cgcctatttg attctttaga ggcggccctg cgattagcag acggctatgc tctggttgac
    12301 gtgatcggtg aagaagaaat gttgtttagc gaacactatg cctgtcctta ttgcggattt
    12361 actgttggcg aactagaacc aagactgttc tcattcaatg cgccttttgg agcttgtcca
    12421 gactgtgatg gtttaggtgt caaattagaa gtcgatcaag atttagtgat tccagataga
    12481 acgaagacac ttagtcaagg tgctatcgtt ccatggaatc caatcagctc tcagtattat
    12541 cctcaaatgt tagcgcaggc ttgtgaaagt ttcggtatcg atttagatac accattcgaa
    12601 gaattaccgg aagagcatca ggagatcatc ttgaatggga cagatgatct tttccatttc
    12661 cattatgaaa atgattttgg tggcgttcgc gatgtagaaa cgactttcga aggggtattg
    12721 aagaatatcg agcgtcgtta tcatgatacg aacagtgatt tcacgcgtga gcaaatgcgt
    12781 ctttatatga cagaattaac ttgtcagaca tgtaaaggtt atcgattgaa tccccaagct
    12841 ctatctgttc aaatcaatgg aacgaatatc gggcaaacaa atgagttgtc tattcaaaaa
    12901 gcagtagatt tcttctcaaa cttaacgtta tctgaacaag agaaagtaat tgccaaaccg
    12961 attttaaaag aagtaaatga cagactttca ttcttggaaa atgtgggact agactatcta
    13021 actttgagtc gcgcttccgg aacattatct gggggagaag cgcagcgaat cagattggct
    13081 acacaaattg gatcgaatct ttctggagtt ttgtatattt tagatgaacc atcgatcgga
    13141 ttgcatcaac gcgacaacga tcgactgatt ggctcattaa agaaaatgcg tgatcttggc
    13201 aacaccctcg ttgtagtcga acatgatgaa gatacgatgt gggcagcaga ttatttgatt
    13261 gacgtcggac ctggggccgg agaacaaggc ggacaaatcg tggctgcagg cactcctgaa
    13321 gaagtagcta atgatgaaaa ttctttgaca gggaaatatc tttcagggaa aaaatcaatc
    13381 cctgtaccaa aagaacgccg taaaggtaat gggaaagcaa tcaagatcac tggtgcttca
    13441 gaaaacaatc tgaaaaatat tagtgttgaa tttccattag gagaatttgt agcagtcaca
    13501 ggtgtgtctg gttctggaaa gagtacattg gtcaatagta tcttaaagaa atcgttagcg
    13561 caaaaattaa ataagaattc tgctaagcca ggtaaattca agacaatttc cggctacgaa
    13621 agtatcgaaa agatcatcga tatcgatcaa agcccaatcg gccggacgcc gagaagtaat
    13681 ccagcgactt atacaagtgt atttgatgat atccgtgggt tatttgctca aacgaacgag
    13741 gcaaaaatgc ggggttataa gaaagggcgt tttagtttca acgtaaaagg cggtcgttgt
    13801 gaagcttgtc gcggggatgg aattattaag atcgaaatgc actttttgcc tgatgtctat
    13861 gttccttgtg aagtatgtca tggtaaacga tataattctg aaacattaga agtgcattac
    13921 aaaggaaaaa gcattgctga tattttggaa atgacagtag aggatgctgt agaattcttc
    13981 aagcacattc caaagattca tcgcaaactg caaacgattg ttgatgttgg cttaggttat
    14041 gtgacgatgg ggcaaccagc aacgacattg tccggtggtg aggcacaacg gatgaaactt
    14101 gccagtgaat tgcacaaaat ctctaatgga aagaatttct atatactaga tgaaccaacg
    14161 acaggacttc atagcgatga catcgcccgc ttgttgcatg tattacaaag attagtagat
    14221 gctggtaaca cagttttagt gattgaacac aatctagatg taatcaaaac agcagattat
    14281 atcattgatt taggaccaga aggtggagaa ggtggaggaa cgatccttgc gactggaaca
    14341 ccagaagaaa tcattaacgt aaaagaaagt tatacaggtc actatttgaa aaaaataatg
    14401 gtataaagag gatagatagc tgagatagtt aaaaaaacat aaaaaaatga atttttgctt
    14461 gttttattaa tatcagtgtt ctagaatcat tacatggtag ttctttctac actgattggt
    14521 atagataaaa acttcctata agttcaatcc gttcaaaaaa gaattgagca atcggaggag
    14581 tttcctttga ttgctttttt ctttatggta tgatattcct tgtgtagttg gaaaagaaaa
    14641 acaagagatt atgagtaagt gagagataat gatgaacagt attgaaaatg aagaatttat
    14701 ccctatatta ttaggaagcg acatgaacgt ttatggtatg gcacgttctt ttaatgaagc
    14761 atacggcaaa atctgccaag catatgcttc ggatcagtta gcccctaccc gctacagtaa
    14821 aattgtcaat gtagaagtta ttccaggatt tgataaagac cctgtattta tcgagacgat
    14881 gcttcgtttg gcaaaagaaa gatattctga taagagtaaa aaatatcttt tgattgcttg
    14941 tggtgatgga tatgcagaat tgatctccca acataaacaa gagctgtcag aatattttat
    15001 ctgtccatat attgattatt ctttatttga aaggctgatc aataaagtaa gcttttatga
    15061 agtttgtgaa gaatatgatc ttccgtatcc taaaacattg atcgtcagag aagaaatgtt
    15121 agtaaatgga catttggaac aggaattgcc ttttgaattc ccagttgctt tgaaacctgc
    15181 aaacagtgtg gagtatcttt ctgtccaatt tgaaggaaga aaaaaagctt ttattttaga
    15241 gactagagaa gagttcgatt taattttagg ccggatatac gaagccggtt ataaaagtga
    15301 aatgattgta caagatttta tcccgggtga tgatagtaat atgcgtgtgc taaatgcgta
    15361 tgtagatgaa gatcatcaag ttcggatgat gtgccttggt catccactat tagaagatcc
    15421 tacaccagca tcgattggta attacgttgt gatcatgcca gattataacg aaaaaatcta
    15481 tcaaaccatt aaggcatttt tggaaaaaat cgaatataca ggctttgcca atttcgatat
    15541 gaagtatgat cctagagatg gagaatataa attatttgaa atcaatttga gacaaggtcg
    15601 tagcagtttc tttgtgacat tgaacggctt gaatctggct cgttttgtca cagaagatcg
    15661 agtgttcaac aaaccctttg ttgaaacgac ctatggaacg aatcaatcag ataaagcaag
    15721 attatggatg ggtgtgccca aaaaaatctt tttagaatat gctcgggaaa atgaagacaa
    15781 aaaactagct gagcaaatga tcaaagaaaa tcgttacggt actactgtat tttatgaaaa
    15841 agaccgatca atcaagcgct ggttgttgat gaaatatatg ttccataact atattccaag
    15901 attcaaaaag tattttcatg tgaaagaagg ctagttcatg gagaattttt tcagtatttt
    15961 aggcggaatg ggcacgatgg cgacagaaag ttttgttcgc ttgatcaacc accgaacgaa
    16021 agctacaaaa gatcaagaat atttgaatta tgtcttattc aatcatgcaa cagttcctga
    16081 tcgtacagct tatattttag atcgatcaga agaaaatcca atgccgtttc tgctggatga
    16141 tattgagaaa caaaatttat tgcgaccaaa cttcattgtg ttgacttgta acaccgcaca
    16201 ctactttttt gaagagttac aagcggcaac ggatattcct atcttgcata tgccaagaga
    16261 agcagcaaat gaacttgtcc gtcagcatac gacagggaga gtagcgattc taggaacaga
    16321 aggaagtatg aaagctggaa tctatgaacg cgaagtcaaa aatcttggtt tcgaaacagt
    16381 gattcctgat actgcgcttc aagaaaaaat caattacctg atctatcatg agatcaagga
    16441 atcggattat ttgaatcaag agctctatta cgaaatctta gaggaagcgg tagagcgcct
    16501 aaactgtgaa aaagtcatcc taggttgcac agaattatct ttgatgcatg aatttgctga
    16561 agataatcat tacccagtga tcgatgctca atctatttta gcggatcgaa cgatcgaacg
    16621 tgcattagct gaaagaagcg aggcattgga tacagcatcg gaaaagtaaa aaaattggat
    16681 ttggggcaaa tatcctaaaa gatatttggt ccaaatcttt ttgtaaaaga aaaagaaaac
    16741 tggtaatgat acgcccggga tgatataatt ttattatgca caatgtgaga ggggttcgta
    16801 aatatgacag ataatttgca attagtcatc ataacaggta tgagtggtgc agggaaaaca
    16861 gtagctatcc aaagttttga agatatgggc tatttttgta tcgataatat gccgcctcga
    16921 ttgattccaa aattctggga attgatcaaa gaatcaggta aagtcacaaa aatggcttta
    16981 ggagtggatt tgcgttctcg ttccttcttt gaagaaatcc aaaacatgct gatcgaaatc
    17041 gaaaatacga acttcatcga cacttctatc ttatttttag atgcttcaga cgaagaacta
    17101 gtggctcgct acaaagaaac aagacgagca catccaatgg cgatggacgg tttgatcact
    17161 gaagggatac gtaaagaacg ggcgatacta gacgacttga aagcccaagc ttccgtgatc
    17221 attgatacga caacattgac accacgacaa ttacgtgaaa aaatcaatga gtcattcaaa
    17281 tcaaagggag accaaggatt tcggatcgaa tttgtatcat ttggttttaa atacgggttg
    17341 ccgatcgatt cggatattgt gatggatgtg cgttttttgc ccaaccctca ttatatcccg
    17401 gaactaagac cattgaccgg aaaagatcag ccagtctatg attatgtgat gtcctttgat
    17461 gaaacgcaat cattctacca taaatttttg gatcttctgg aaactgtatt gcctggatac
    17521 attaaagaag gaaaaagcag cttgacagtt gcaatcggct gtacaggcgg tcaacaccgt
    17581 tctgtggcat tgactgaacg tttggcaaat gctttaggga aaaactataa agtcaatgtg
    17641 acacatcggg acaaggataa aaggaaagag acggtgaacc ggtcgtgaaa atgaaaacct
    17701 accggataag aaaaccaaag atcgtagtgg tcggcggtgg cactggtctg ccagttatcc
    17761 tgaaaagttt acgaaatcaa ggagcagata tcacagcagt agtgactgtt gcagatgatg
    17821 gtggaagcag cggtgcaatc agagaatcga tcgctatggc tcctcctggt gacttgagaa
    17881 atgtcttagt ggcgttatcg gatatgccac aattctatga agatattttc caataccgtt
    17941 tcaaaaagga agatcagttt ttagccaatc atacgatcgg taatctgatc attgccgcag
    18001 tttccgagat gcggggaagc acttatgagg cgattcaatt attagcaaaa atgatgcatg
    18061 tagatggaca tgtttatcct tcttcagaaa ctcctttgac cttgcatgct gtttttaaag
    18121 acggaacgac tgcagtagga gaatctaaaa tcgcgatcaa ccgtaaaacc attgaccgtg
    18181 tgtatgtctg caacacaaat gataaaagtg aagccaaagc ggctcgaaaa gtcgtttcag
    18241 caattatgga cgccgacatg gtcgttttgg gacccggaag tttattcacc agtatccttc
    18301 caaatttagt gataccagaa atcggcgaag caatgctcaa gacaactgct gaaaccgtct
    18361 atatctgtaa tatcatgacc caaaaagggg aaacggaaca tttcacagat gccgatcata
    18421 tacgtgtttt acacaagcat ttgaatcagc cttttatcga tacagtattg gtcaatacgg
    18481 aaaaagtgcc tgaagattat atggacccag agatctatga tgagtatcta gtacaagtcg
    18541 cacacgactt caacggattg agaaatgaag gatgtcgagt gatttccacc gactttttag
    18601 aattgaaaga tggtggtgtc ttccacgatg gagacaaagt agtagaagaa ttgttccgtt
    18661 tagttttcgg ttcaaaatat taagcaattt attgttggag aaaggaggag aataggatgt
    18721 catttgctgc agatgtgaag aaagaactaa ctggtttgga agttcatcga gaacatgcga
    18781 aagcggaatt agctgctttg ctgcgaatga acggctcttt aggtattgtg aaccagcagt
    18841 ttattttgaa cgtccaaaca gaaaatgctg caattgccag acgtatctat tctcttttga
    18901 aagatcatta tcaggtacga agtgagctac tcgttcgtaa aaaaatgaaa ttaaaaaaga
    18961 acaatgtcta tatcgtccgg ttgaagcaag gcacaaaaga tgtgttgatg gatcttgata
    19021 tcatggatgg tatgatgttc aatggacacg tttcggacga gattatggga aattcgcaaa
    19081 aaatgcgttc ttacttacgt ggtgctttta tggcctcagg ttcagtcaat aatccggaaa
    19141 cgagtcgtta tcatttggaa atattttcta tttatgaaca gcataaccaa gacatttgcg
    19201 atatgttaaa ttattacggt ctacatgctc gaacgcttga acggcgcaat ggctatattt
    19261 cgtatttaaa aggagcagaa aaaattgctg atttcttaac attgattggt gcaaccaact
    19321 caatgctccg atttgaagat gttcgaatcg tacgggatat gagaaattcg gtcaatcgtc
    19381 tagtcaattg tgagacagca aatctgaaca agacgattga tgcggcttct aaacaaatcg
    19441 aaaacatcga attgatcgaa gcaaaggtcg gtctacatgc ccttcctgaa aaacttcagg
    19501 aaatcgcaga acttcggctt caacatccag aggtcagctt gaaagaatta ggggaaatga
    19561 ttccctcagg tgcaatatca aaatcgggaa ttaatcaccg aattcgcaaa ataaatgaat
    19621 ttgcggaaaa actgcgtgaa gaagcagttt aatccaaaga aaaaactgat acatgaagca
    19681 ggagatgcga atgtatcagt tttttttaaa agctatactt gcttttcttc cgtcaaacta
    19741 taattccaac tttactggag taacataatg ttgaaaggct tctattgttt tttaatacaa
    19801 tcgtataaaa agcggggaga caactgaatg aagattttag ttttatacat aaacttttgc
    19861 ttaatataga agtcctgata tagataggat caagtattta tctataactg gagattaatt
    19921 aaaaactgga aggaactgct cgatcaacct tgagaactgt ttttcttact tctggtcaag
    19981 attctcgtgg ttggtggata aggatggttg ataagttcat tggattagct agtcgaaaac
    20041 aaccaggtgt aattaatgta gaagttttaa caaggggatg gagtgattta ttcagtgata
    20101 atcataagtt cataaatagt aattgggatg tatggttggt atacgttata cctgaacttg
    20161 aactatcaag atttggttta gaatttgaga atcgatgttt ataaagggag ggcaaaaaat
    20221 gtgctttttt acaactttta ctatcattat gattgcagta ctttttattt ttctagatat
    20281 agcaaagcga aatacagcat ttttattata tcgggtttta cttcgagcag gtctcataac
    20341 atttatatcg atcgttggat tttttctatt cacagttatt gtatttattt ggagaactac
    20401 agcaccacca ttgccagaga ttacatatgg agaatttccg tttagtcttg aatatgaaat
    20461 agatgaggaa agaattttga ttgaagatac ggtgattgca gaatttatag gaagttttag
    20521 agggaatgtg acaactcaac ctagacgaag ttggagtata aaacttttaa ataatagtgg
    20581 agagttgaaa gcatttcatt atgaattctt aataatagaa agtgaagatg taacaatcac
    20641 tttaagtagt ggctctgctg gatattttat gaatgatggt ggaagcaagc cacagctcac
    20701 agtccgtgat cgtattgcag gttttatgca tttttctcac tatgacttag aagaagctcg
    20761 taaaatactt gcagaatatg ggatcacatt aatcaattgg gaatatgcgc caccgataag
    20821 aaattttttc aatgattatt attagtctta taaaaaacag aatgtaaaat ttatgtgcgc
    20881 gagtatttag ttttccattt tttgtttgat agatatatag cattgttgaa gcatgggata
    20941 gtggtgccaa ttttatcaat gaggttggtc aggcagttaa cataattggt gatgctataa
    21001 atccatttaa ttggaattgg aggaattagg tgtggaaaca gtgagattat ggatattcat
    21061 gaaaaatttt cgttcggctt tgagatatgt tattctggaa tcaaaatcag gttattatct
    21121 gattgacaca gatcactata ctttttgggg aggctacctt tttccgattt ttaattggat
    21181 aatcccccat aaggttatga aaattaactt gcctgcagaa gaaataaata gattaatact
    21241 caacaaagat ttacaaagga aaagagagaa agaacattct aatgttgctt ggataggtcc
    21301 agcatttgca gtagtgactt cttcaattgt cggtcctatg ctagagagta tttatagtga
    21361 tttttctatt ggaatacgtt atttatttgt tactttaagt atagctgttc ttttgtgcat
    21421 aaagggttat gttagcaaga agaataaaga tattttaaca ctaatcggtg taaacaattt
    21481 atctagtact aaaattatac tgtttcctga atctataaaa gaaggatcaa ggttgttgat
    21541 aatttttata atgcttatac tgttttctgt tttatttggg gtgatgttta taga
//
LOCUS       DAMEET010000048        21333 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.049, whole genome shotgun sequence.
ACCESSION   DAMEET010000048 DAMEET010000000
VERSION     DAMEET010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.049"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            68..808
                     /locus_tag="PB780_001594"
     CDS             68..808
                     /locus_tag="PB780_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDE0733650.1"
                     /translation="MTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGL
                     TTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTP
                     GGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVET
                     QAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGE
                     TIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1232..1402)
                     /locus_tag="PB780_001595"
     CDS             complement(1232..1402)
                     /locus_tag="PB780_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDE0733651.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1596..1775)
                     /locus_tag="PB780_001596"
     CDS             complement(1596..1775)
                     /locus_tag="PB780_001596"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733652.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            2162..3274
                     /locus_tag="PB780_001597"
     CDS             2162..3274
                     /locus_tag="PB780_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDE0733653.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3352..3645)
                     /locus_tag="PB780_001598"
     CDS             complement(3352..3645)
                     /locus_tag="PB780_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDE0733654.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3813..4127
                     /locus_tag="PB780_001599"
     CDS             3813..4127
                     /locus_tag="PB780_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HDE0733655.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4148..6127
                     /locus_tag="PB780_001600"
     CDS             4148..6127
                     /locus_tag="PB780_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDE0733656.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6140..6466
                     /locus_tag="PB780_001601"
     CDS             6140..6466
                     /locus_tag="PB780_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HDE0733657.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6482..7000
                     /locus_tag="PB780_001602"
     CDS             6482..7000
                     /locus_tag="PB780_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733658.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7000..8352
                     /gene="celB"
                     /locus_tag="PB780_001603"
     CDS             7000..8352
                     /gene="celB"
                     /locus_tag="PB780_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDE0733659.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8436..9941)
                     /locus_tag="PB780_001604"
     CDS             complement(8436..9941)
                     /locus_tag="PB780_001604"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HDE0733660.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10033..10623)
                     /locus_tag="PB780_001605"
     CDS             complement(10033..10623)
                     /locus_tag="PB780_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDE0733661.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10802..12448
                     /locus_tag="PB780_001606"
     CDS             10802..12448
                     /locus_tag="PB780_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDE0733662.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12497..13216
                     /locus_tag="PB780_001607"
     CDS             12497..13216
                     /locus_tag="PB780_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDE0733663.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13695..14642
                     /locus_tag="PB780_001608"
     CDS             13695..14642
                     /locus_tag="PB780_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDE0733664.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14654..15574
                     /locus_tag="PB780_001609"
     CDS             14654..15574
                     /locus_tag="PB780_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDE0733665.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15615..17072
                     /locus_tag="PB780_001610"
     CDS             15615..17072
                     /locus_tag="PB780_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDE0733666.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17417..18031
                     /locus_tag="PB780_001611"
     CDS             17417..18031
                     /locus_tag="PB780_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDE0733667.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18024..19748
                     /locus_tag="PB780_001612"
     CDS             18024..19748
                     /locus_tag="PB780_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDE0733668.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19745..21196
                     /locus_tag="PB780_001613"
     CDS             19745..21196
                     /locus_tag="PB780_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDE0733669.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         6876 a         3613 c         4322 g         6522 t
ORIGIN      
        1 ggggtatgaa ttagatgcga caccgatcga gtttgagatc gaaagaagtc agacagcggt
       61 ggtagaattg acgaaagcaa accgacttac accaggcggt gtcgtgttga caaagatcga
      121 tgaccaaagc ggggaagtcc tgcaaggagc tgtttttgag ttacaagatg ccaatggaac
      181 cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgattg acggcttagc
      241 accaggagtg tatcagctgg tagaaaccca agcgccaacg gggtatgaat tggatgcgac
      301 accaatcgag tttgagatcg aaagaagtca gacagcggtg gtagaattga cgaaagaaaa
      361 tcgacttaca ccaggcggtg tcgtgttgac aaagatcgat gaccaaagcg gagaagtcct
      421 gcaaggagct gtttttgagt tacaagatgc caatggaacc gttctgcagt caggtctaac
      481 cacaggagca gatggaaaac tggcgataga tggcttagca ccaggagcat atcagctggt
      541 agaaactcaa gcgccaacgg ggtatgaatt ggatgcgaca ccagtaactt tcaagatcga
      601 aaaagaacaa gaagtggcga tctttttaac aaaagagaat agaaaagttg ctgatagctc
      661 agatataaga ggaaacaatc caacaagcag tggaaataag catcttccaa aaactggaga
      721 aaccatcatt agccagttta tattgtcaat attgggtgtg ctacttgttt tcatcagtat
      781 aaaatttaga aagaaacgaa atatatgagc taaaataaaa aattgaataa gcatagtttt
      841 taaaatcaac tagtacccat caattgaatg gaaactaccg attttagaag gggtatacaa
      901 acaaatatta gtatcggagt agagaaggtt gaacccaacc agtaaataag taaagacaat
      961 tttgtgctgc gttgagaagt gaaaaataaa cctttgtagt aatactagtt aataaaaaat
     1021 atacaaaaac actgcgaaaa tcattcttga gtttcgcagt gttttttgcc taatgactca
     1081 agtttattgg gtaatacttt gattttcttt ctacaaataa tcagcgatca gttgtaattt
     1141 tttctctagt tttaatggct ctttgtcaat aaggagaaaa acaagtcggg tcttttgtct
     1201 cggaaagctt caaccaactc gtgaataaag gttacgcctc cttgaaaggc gtggaaaaac
     1261 caagtaatcg atcaatcacc aatttcgcat ttggtagaac atgtttggtg agctgaaagt
     1321 aggtggcgtt catgtctgta actaagaatt ctatttcttt aggattagtg cagctaagga
     1381 aatagtttgt taatcgagga attttttgtg taggtaaatt ttctcgatta atttttctgt
     1441 ctcgccatcc gcggaaataa aactcagttt atcctcaacg gaaacgtgag aacgaaattc
     1501 acaaccctca acactcaaga cagaattctt ttggaagact ttggtaaata gcttctcaac
     1561 tctttcaatg tgcgaataac ggttgtcaaa gatacctaac aacttttcga aataaaaaat
     1621 aacgacactt tttcagtcag taaagaagtg attttatgtc tgacatgatt tgaaatcgag
     1681 tgccttggac ggataaaata actttgagcg ttccaatggc ttcgacagtt tttacaggta
     1741 tagcgctgct tttttaggcg tataaccatt ggcatatgat tatattattc aaagtggaca
     1801 atggtttcct tcttcttgtt tttcaccacc attaatttcc tatttccatc taccacagca
     1861 gaaccacaat ttctacagac acatggagca gggaagagaa ctgcagcaac gatcaatgtt
     1921 tttttcttct gtaaggtttc gtaagagacc tctgtaatca tcaaattttt gtctattgat
     1981 ctcagaattt ttttgataga accattcata aagcatatcg ttctctcagt tgtttatttt
     2041 gtggtgattt aatcatacaa gaaaacgata tgttttttta taaaaacatg aataggaaaa
     2101 atagttgaca agaaaatgac aacgccatat gatgttccat agtatttaca acctgtcata
     2161 gatgactgta aaaaataaaa gtaaaaagaa aggacgacaa caggtggatt tttatcagtc
     2221 tctacaacta gatccgttta ttttaaaaca aaaaatacga gaagcagctt ctaaaaaaga
     2281 aaagtgcatg tatatttgtt cattgttttt acgatcatta ttcatcgtat tgtttgccat
     2341 ttgttttatc attataatta caacattatt tgaatcgaag cataaaccat acgctgtggt
     2401 tttattctgt atgttgatga gtattcgttt tgttgacttt ggttataaga tttcccattc
     2461 gatcataggt ttagcaatcg tcatgttctc actattagta gcaccgtatg tccagctgat
     2521 caaatggtca gttatgggca tgttgattca ttttatttta ctaagcagca tattaatggc
     2581 aaccgctagt gatcccaaaa tggggaatgc ttctttgtac ggcttttcat atctctttat
     2641 tgtttattcg ttacctaaag attcgttgaa caagaatttc tttacacaaa caagtagctt
     2701 attatttttg tttttttgtt ggttttcagt tctcctttac agaaaacatc gagaaaagaa
     2761 caaagacaaa agcttgttta aagagatttt tttgaaaggc atgtattcgc aggaaaaaat
     2821 ttggatgctc atctatgcat ttggcattag tcttttgatc gttgcaggag agtatgtccc
     2881 ttttcagcga ttgatgtggg cagggtttgc attttcttct attgtatcta gctatggatt
     2941 gatgtcgatt ggtttcaaag aacgagcagt agaccgaatc attggctcat tgattggatg
     3001 tgttttattt attggtatca gccaatttat tccttttaac tgggtcggta tattaggtgg
     3061 tctagcttta ggtgtttgtt ctacctatag atataaaaca gtttttaatt cttttggtgc
     3121 tttggcaatc actgccagtt tattcggggt tccaggagca gtaacgatcc gcatttttga
     3181 gaatatcctt ggggtatgtt taggaatcat gtatattggg gtgactgaaa tacttatccg
     3241 aaaaataaga gaaaaacacg gtttgaatca ttagatgaga tcgaataaaa aataaggaaa
     3301 aagtgaagat acacatggat gataaattgc tatcattcca tgtgtatctt tttacggtaa
     3361 atcagtgaaa cgttcgactt tcatttttaa tttatacttt ttacggagtt cgtttatttg
     3421 agccatcatt tcggatttgt gatgcttttc gattgcttct tcatctttcc aacggtcgat
     3481 cagtaaaaga gattctggat cttctagtgg aaagtaatag tcatatttct cgtttccttc
     3541 ttctgcacga atcgctttga caatgtttct ttcgaccatt tcttctgcaa attttctggc
     3601 ttgtccattt gtacttgtat acaaaatatt cactgtgata ctcactaaat cccctcctga
     3661 aaaaattcta tcactagtat atcacggtgc cagaaattat tattcaagat atgatacaca
     3721 ttttttccgc aatgctacgg aaatatatat tttgaataga aaacgcttac aagatatgct
     3781 tggtttataa aaataaaagg agtgattgat ttatgaaaaa agcattgatt atttgtgcgg
     3841 caggaatgtc atcctcgatg atggcctcaa agacaacgga tttctttaag caaaaaagag
     3901 aagaggtact cgtagatgca gtttcagcta cagaaggcga taatatgatt aagaatagtg
     3961 attttgattt atttctgatc agcccgcaaa cgacgatgtt tttggataaa ttcaaaaaat
     4021 taggagaatc tgttggaaag cctgttgtta gtatcccttt ccaagcgtat gtcccaatcc
     4081 ctacaggcat ccagaagtta gcagaggtga tcgaagagaa catatgatgg aataggagtg
     4141 gatgataatg aaacagaagg aaaaattgct gttgagatat ttgattgatc acaagaagga
     4201 gtatgtcacc agtcaacgtt tagcttctga attgttatta tctgatcgga caatccgtaa
     4261 ttacttgcag cggatcaaag agttagtaga aaaaaacgga ggtaagatca ttgctaaacc
     4321 aggttatggc taccaactgc atattttaca aagactgaca tttgacttgt ttttgagtcg
     4381 gcaagaaata gtacctggat atgctagaga accgcaagga ttttatgagt ccgaagatcg
     4441 acaaaagtat atattgaacc aattgctttt ggaagatgcc attcttttta tggatgattt
     4501 ggcagaagag ctttatatca gtcgttccag tttgacaaag gacatgcaag aaatcaagga
     4561 gcgattgatt ccatattcac taaaaatcgt ttctaagcat ggtcaaggga catggatcga
     4621 aggggaagaa agaaatcgac ggcattttat catggatact ttttttggaa accattatgg
     4681 aaattcattg aaagaatatt tagggcaaag ccgttttttc ccagacatca gttttgaaga
     4741 gctagtcatt atcattctcg atgaaacaag agaagctaag ttaaaagttt cggattttat
     4801 catacagaat ctgactttac acttggcttt ggggatcaaa cggctccgag aagggtttga
     4861 aatcaaggaa ttaggcatcg aacaagaagt ttctaagcga gtagagtatc aagtggcgca
     4921 gcggatcgtt caacgtattg aggtaatcgc aggtgtatcg tttcccgaag aagaaattgc
     4981 ttatttgaca ttacatctca tggctaagtc gaatcacaaa gaaaatcaaa atgatcagga
     5041 actatcaaag gaattagcga aagttctgaa aagattgtct caggaatttc atttttcatt
     5101 agtagaggat ctccagcttt tcaatggact actcgatcat ttgtcaccga tgatgattcg
     5161 attgagccga ggtattcgca ttgagaaccc attgactgag gagataaaga aagaaaatcc
     5221 caaagtcttt gacgcagtaa aaaggcattt cagcaacatg ccggctttga aaaactatac
     5281 catcaatgaa gatgaatggg cctatttggc tttgcatctc atggcggcat tggaaaaaga
     5341 aagagctgcg cataaactac atgcattgat tatctgtgca acgggttacg gcagtgccca
     5401 actattaaag aatcgcgtag tcagtgaatt cggtaaaaat attacagtgg taagtgtaaa
     5461 aggctattat gagatcaatg aaacaacttt aaaaggaatc gacctgatta tttcttctat
     5521 tgatctttcg acaatgtttt tcaaaattcc agttttacat gtcagtattt ttttgaatga
     5581 agatgatgtt cgtaaaatac gaaaggtagt tggagaatct ataccctatc attcctcaga
     5641 tagaccgata ccagcatttt ctctacagca aaaaaagcag atttacaccg aacagttgtc
     5701 tgaaaaattt ttcaagactt atacgtacga accgaaaaag gaagaaattc ttcatgattt
     5761 gctgcaaaag ctgagcataa atgaagaaga aggatatata agagaaatgc gaaagcagat
     5821 ccagcaacgt gaaaatatgg gacaaatcat attcagtgat acagtggcag tcccacatcc
     5881 cgctgttcca gttggaatcg aaacaaaggt agccgttgca ttgattcctt ctgggatgca
     5941 gtgggatcaa tatcaagaaa ttcattttgt ttttttagtt tcgccttctt atgtcgaaaa
     6001 tgaaggaatt acggtcgtga ctaaagcaat cgttaggtta gttgaccagc taaaagtaca
     6061 gcaggaaatc ttagctgaac ctatttttga gaatttcagc agtaaattta tcaaactgat
     6121 cgaataagga ggaaaacaga tggctgaaaa aataaacagt gaaacactac aagtgacttc
     6181 ttttgatatc attttccata gcggaaatgc acggacactg atacatgaag catttcgctc
     6241 gatgcgtaaa gagatgtttg aagaagtaga agagaagttg cgacaagcaa atgaagagat
     6301 tcttcatgcg catcagtcac aaactcaact tctgaaagaa tatgccagcg ggacaaaaat
     6361 agaaatggaa attatcatgg tccatgcaca agatcatctg atgacgacga tgacattatt
     6421 ggaagttgca caggaaatga attatttgta tcaaaaaaat aaatagaaag gaaggatcat
     6481 tgtgcaggat acagtaaaaa ggaaaatcga attagaaaaa aagcaactta gtttgaaaaa
     6541 catgtatttc aatcgttatc taatcgtacg ttacctgact gcctttttct ttttcatgaa
     6601 catgcaatgg atgattctgc ttctatcagc taaaagttta ggaagtagtt taccgatgat
     6661 tctgatttta gctattttgc ctgcagtagg agaacaagta aagctttatc gaaaacacca
     6721 aacgaacgtt ccatggacaa agtggtattt tttgttccag agtgtcttta atttattagt
     6781 gattccagtg ttgtttacct caggatttac attcttgtat ccttttatgg caaataacaa
     6841 tggaggacag ttatttgttt tcattcttgt tgtaagcggt atttttgcca gtgcatttat
     6901 acagtatcgt ttaaagaaaa tttcactcaa ccaagaccaa cagtatatcc gtattaaaca
     6961 atatgaaaaa gcgttatatt taggaaagga gaataactaa tgtttgattt tttgcagaaa
     7021 tacctgatgg gacctatggg gaaaattgcg caacttaaaa tcgttcgggc tgtcatggca
     7081 gcggggatgg cttctattcc tttcacgatt gtaggctcga tgtttttggt tttaaatatt
     7141 ttgccattac cgttcccatt tttagaagga ttttttaatg caactttttt caaagttagt
     7201 gacttgtaca tgatcgtcaa tacaatgacg atggggattt tatctattta ctttgccatt
     7261 gtgtttgctt atgaattaac gtcaattgaa cgagatgaac aaaaattaaa tgttaatccg
     7321 ctcacggggg cattactatc tgtttttgca ttctttatgt gtatccctga attgatcatg
     7381 actgatggta aaatcagctt gattgcttct atcaatgaga cagaaactgt agtaaatggt
     7441 attaggatgg gctcttttgt cgaacgttta ggaacttcag gaatctttac agctattatt
     7501 atgtcgatta ttgcggtcga attgtattgt atgtgtgtca agagaaattg ggtcataaaa
     7561 atgccagatg ttgttccgcc aggagtttct cgttctttca ctgctctgat cccaacattt
     7621 gtcattgcgt ttgtggtgat gatcatcaat ggattattag ttgcactagg aacggatatc
     7681 ttcaaaatga ttgctatccc atttgggttt gttacgcagt tgacgaatac atggcttggt
     7741 gtcatggtga tttatttctt gatccatgca ttgtggatcg tagggatcca tggagcgaat
     7801 atcatcactt ctttcttgac cccgatcgta ttagctaata tggcagctaa cgcacaaggc
     7861 gcaaattatc cgttggcagg agaattcaac aattcttatg taaccgttgg tggatcaggt
     7921 gcgactttag gattgatcat ctttattgca ttcctcgcga aatctgatca gctgaaagtg
     7981 ttaggaaaag cctcacttgt accaggatta ttcaatatca atgaaccaat cattttcggg
     8041 atgccaatcg tctacaatcc ctatttagcg gttccgtttt tcttagctcc gatggcttct
     8101 gctacactcg cttattttgc cattcggctg gaaatcgtca aaccgatgct tgcacagatg
     8161 ccgtggcctt caccagtcgg gatcggagca tttgttggta gtggtggtga ttggaaagct
     8221 gctgtgttag cagtcatttg tgcaatcatc gcattcatta tctggttccc atttatcaaa
     8281 ttctatgata ataaattatt aaatgaagaa agagaaaagg cagcagaatt agctagtgaa
     8341 aagttaaaat gatttctaag aaaaaaggaa ctgtgacaaa aaatcacagt cccttttttt
     8401 cttagaaatg ggaaaccact agtatataaa taccattatt cttcaatcaa ccgcttcacg
     8461 attgcataat ccccttcata aggttcatct acaccattga ttttttgata gggatccacg
     8521 ccttttcccg caataacgac tgaatcttgc ggagcgcttt ctgctaaagc gcgtcgaatc
     8581 gcttcaggac gatccatttc ataatgaacg atcaaatctg gattagtaat tgcttcattg
     8641 atttcttccg cgattttttt cggatcttca aatgctgggt catctgccgt caaaaaggcg
     8701 atgtcagctg tttctgatag cacttttcca aaatcatgtc gacgggagat tgctttgttt
     8761 cctgggctgc ctaatacaac aattacacgg ccatctggat gttcattttt ggcaaatttc
     8821 agcaatgttt tcaagcttag ataattatgt gcatagtcca catagacatg cgctccattg
     8881 gattggatca attgatccat tcggcctggt acacgagctt cttttaatcc gtcttgtgca
     8941 tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg cactggctgc attttcttgg
     9001 ttgaatcctc cagccagcaa aatatcataa gcagtatcgc agatagctaa gcgatcttca
     9061 tgagaagata aagtaaaacc gtgagtacct ttatctgaac ggatcacttg ataattgctt
     9121 tcttccgaac gaccataagt gatcgttgga atctggaata gatcggctgt ttccctaagt
     9181 aaatcaaaat agtcactttc atgatttaaa acaactgttt ttgaattcaa gatcaattgt
     9241 cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga tcggactgat atggtcaggc
     9301 gagatattca agaagattcc gacatctaaa gtcaagccgt atacgcgttg tgttttgtaa
     9361 gcttgtgaag aaacttccat cactagatga gtcataccat tttcaactgc ttctgacatc
     9421 atacgatata aatctaatga ctcaggcgtt gtcaaatggg atttgaaata tgttttgcca
     9481 tctaacgttg tattcatagt agaaagcaat gctgtttttt tatttgttgt gtgatccaag
     9541 attgcttttg taaagtacgc agcagttgtt ttaccttttg ttccagtgaa tccgatcact
     9601 ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa gaagcgccat tgctttacgg
     9661 atatccgtta cgatgattcc tactgctgaa ttttcgtatg gttgttctga tacataatat
     9721 tgaataccct gttcgattgc tgaatccaaa tattcttctt tgaaattcat tcctttacag
     9781 aaaaacaaag tagaagcatc agcttttctt gaatcataac ttaactgaag aaattcagct
     9841 ttttctggta catcaagatg ccagcctctt gttgaaacaa attcttttaa tagattttct
     9901 tttaatagta aatgacggat gtcttctaaa gagaagctca ttgattgatc cctcgcttta
     9961 ttcaatttct tggtttatta tagcacactc tttttaatat atcgtttgtt tccgctatga
    10021 caaaatccag ctttattcaa attttcttga cacaggggac aagcggatga gtattttcat
    10081 aaacaatgaa ctgactgtaa tagataggag aacgaatagt gtctccaaca atccaaaaac
    10141 ataataattc aaaaagacga taagactatt ggtaagtaaa agtccttgcg gcattggaag
    10201 ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata ccgccatttg aggccccgac
    10261 tgtgtacaat gtgtaaaccc caagactgaa caaaagactt cctgaaatca gtgaaaagaa
    10321 atgtgaggag atcatttcca cttttggaac aatctctaaa acaacaggaa aaagcagact
    10381 tcccgctaca gtcttaaaaa atactgattt tcctaatagg acagcggcta gaagcagcat
    10441 cataagattg atgctccaaa cagtaactga gcgttggata gaaaaggccg tttcaactaa
    10501 atgtccgatg gcgcccactc cggcggaagc tatatcgtga gggagcaaga accaattcaa
    10561 tccaactgtg ataaagaaaa gacctaaaaa tataacgggt agttttttta tttcttgtgt
    10621 cattgatatc tcctataaat gttagccata aaaggctgta tatttcttta ctttaccaaa
    10681 aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa agttatataa aatcgattgc
    10741 cagtttctgt tttaccaatt aaaatgatag taatgttttg tagaaagttg gaatctgacc
    10801 gatggataat caaagaaaac cagcattaga tcaattaaca ccgcaagaaa aaatggcacg
    10861 aggctctgct tggatgaccg ccagtaatat catttcaaga ttgctaggcg ctgtctatat
    10921 cattccctgg tatgcttgga tgggcgaaaa tgctaaggct gccaatggat tattcaatat
    10981 gggatacaat atatatgcat tgtttctact gatctctaca gcaggaatac ctgctgcgat
    11041 tgcaaaacaa actgcacgct ataattcatt gaatgaatat ggcacttcac gacgattatt
    11101 tatccgtgca ttacagatga tgggcgggtt aggcctgttg tttgctttgt tcatgtacat
    11161 cgcctctcct tggctagctc atgcttctgg tggcggagaa gaactgattc caacgatgcg
    11221 ttctttgagt atcgctgtat tggtttttcc ttgtatgagt gtgatacgag ggtatttgca
    11281 aggcaatcaa gaaatgatgc cttatgcgct gtcgcagatt gtagaacagg tggcacgtat
    11341 tttttatatg ctattggcta cattcatcat catgaaagtc atggatggtg actatgtgac
    11401 ggcggtcacg caatcaactt tcgcggcatt tatcggtatg ctagcaagta ttcttgtcct
    11461 tttgtatttt ttgaagaaac atcaagcgta tacatcggcg tttattcact acagcgaaaa
    11521 caaaataaat attgcaacga aggaattgtt gttagatacc gttaaagaag cgattccgtt
    11581 tattattgtg ggttcagggg taacgatctt caaattagtc gatcagttca cttttatgaa
    11641 gatcatgtct gcatccaccg agtattccaa tgcacagtta ctggatcttt tttcgatttt
    11701 tagtgccaat ccagataagt tgacgatggt agttattgca ttggcaacat cgatcgcgtc
    11761 tactgggtta ccgttgatta cagaagctgt tacgttgaaa gatcgagtag gtctggcaaa
    11821 attgacaagc ggaaatttac agttgttttc attttttatg tttccggcga catttggtgt
    11881 tatgttactt gcctatccgc taaatacatt attttataca ccggattctc taggaagtaa
    11941 tgtactgatc caagcaagtt ttgtaggact cttcctagga ctatatatgc tagtttccaa
    12001 catgctgcaa ggaatgtttg aaaacaaggc ggcaattaat tatttggtta ttgggtttct
    12061 agtgaaactc cttcttcaat acccagccat tcgtttattt gaagtatacg gccctttatt
    12121 ggcgacaatg atcggatttg ctgtgtcttg taccttgatc ttaaagagaa tccatgaagt
    12181 agcccgcttt aaaccaagtt ttgtctggcg caggacattg ctgatcttta ttcttacctt
    12241 gattatgctt ttggcttcgt ggatcacaaa gatgattttt ggtgcttttt taaacgaaga
    12301 tagcaaattc cagtctctat tgttgattct tgtagtagca ggtatagggg ctttagtgta
    12361 tggttattta gctttgaaaa tccgcttggc agaaagacta ctaggaccag gaatagcgcg
    12421 tttacgacat aaattaaaaa taaaataagt gaatcagaag agctagattg atgctctttt
    12481 gaaatggggg aaacgcatgc gtttagataa gtttttagca gatatgggac ttggcagccg
    12541 caaagaagtc aaaggattaa tcaaaaaagg aacgatcgaa gtcaatggac aagtggtgaa
    12601 aagcgataaa gcgcaagtaa atgaattcga agatgcggtt acttatttag gagaaatcat
    12661 tacgtatcaa aaagattttt actacatttt gcataaacct gcaggagtca tttctgctac
    12721 tcaggataat catgatcaaa ccgtaatcga tttattggaa gaccaggatt atcgggaaga
    12781 tctttttcca gtcggccgat tagataaaga cacagagggc ctcttgattt taacgaatga
    12841 cggggtgttt gcccatcagt tactatcacc taaaaaacat gtggagaaag aatatctagc
    12901 tgaggttagc ggtataatga cagaagaaga tatccgttta tttgcagaag gactgattat
    12961 tgatggcgat gagcagacat tgccggcaaa actccaaatt gatcaagtag atcaagagac
    13021 agaaacttca attatccgtc tgattttgca tgaaggaaag tttcaccaag ttaagcgaat
    13081 ggtcaaagca gtaggaaaag aagtcttata tttaaaaaga atccgtatgg gaaaattttg
    13141 gctgcctaaa gatttgaata aagggcaata tcgacccatg accaaagaag agctagaaca
    13201 agtgagagaa aaataattga atggatagct aaaagaataa gcagagtagt tgtagcgcca
    13261 tctgaaaagc agacttgtcc gtctgacttt cggatggctt ttttgtgaaa tttcggctta
    13321 tttccgtaga agctacttct ggaacaccgt ttgttcggaa taagggactg tgacaagact
    13381 tttgttacag tccccattgt tccttgtgac tcagagttag atagtaagga cacgatctcc
    13441 ctaatactca ggccaaaacg ctgaattcac aacctctgat aaacggtgtt caatcagctg
    13501 accctcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttccc gtttttttct
    13561 ctaatactca ggccaaaacg ctgatttcac aacctctcct atactttgtt tcattttccc
    13621 tatactttgt ttatttattc tcattattga aagcgctata attacgttgc gaatgaatcg
    13681 aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca tttttttatg aatctatggc
    13741 gttataaagc acttgtcctg atggctgtac cgggtatgct ttggatgatt tttttctttt
    13801 acatacctgt aatggcgaat gtcgtagcat tcaaaaattt tcatatttca gcagatgggt
    13861 tcttggctag cttgagggaa agtccttggg tgggttggga aaatttcaaa tttttatttt
    13921 cttcggatca agcatttctc atcactaaaa atacaatttt atataatgta gcatttatcc
    13981 ttttgaatct gctgatctca gtcgtatttg caatcattat gagcgagttg agaaataaac
    14041 gactcgttaa agtttatcaa acgatgtcct tactacctta ttttctatct tgggtcatta
    14101 tcagttactt tgtttacgct tttttaagtc ctgacaaagg aatattcaat cagtggatca
    14161 ctggacatgg aggagaagct gtcaattggt ataatgaacc gaaatattgg ccgtttatct
    14221 tggtgttcat tggtacatgg aaagggattg gttataacag cattatttac tttgcatctg
    14281 ttatgggaat cgatccgact tattatgaag ctgctatggt tgatggagct agcaaatggc
    14341 agcagatcaa acatgtgacg ataccacaac ttattccact aatgacgatt ttaacgattt
    14401 tagccgtagg aaacattttc cgagctgatt ttggcctgtt ttataacatt cctaggaatt
    14461 ccggtgcatt atatgatgtg actcaagttt tggatacgta tatctacaat ggcctgacat
    14521 ccacaggaga ttttggtatg acggctgctg caggacttta ccaatcagtt gtcggctttg
    14581 tcttgttgat gatcacaaat acgatcgccc gacgttttga tagcgattct gcattattct
    14641 aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc caagtccgct ctttcaataa
    14701 gacggctaat tttttctcta atataatgat tgcactcttt gctatttcct gtattttgcc
    14761 atttatattt gttatcgcca tctcttttac cgatgagtcc atattgtcga ctcacgggta
    14821 cagtttttgg ccagaaaagt tcagtatatt tggttatacg tacttgttcg accagatgca
    14881 ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt actgttttag gtacactgat
    14941 caatagcacg gctacttctt tatatgctta tgcgatttca cggtcaaact ttccattccg
    15001 tcgttttttt accgtttttt gtttgattac gatgttgttc tcaccaggga tggtggccaa
    15061 ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg atttgggcat tgatcttgcc
    15121 aatggctgtc agtcccttca atattattgt gatgcgcaca ttctttaaac gttcggtctc
    15181 tgattcgatt attgaatcgg cacggatcga tggagctagc gaactgcgaa tatttgttca
    15241 gatcgtttta ccgttagctg ttccaggtat tgcgacaatc agtttatttg ccgcattggg
    15301 ttactggaat gactggttca atgctttact atacatccaa gatgataagc tagttccact
    15361 acagtatctt ttgatgaaaa tccaaagtaa tattcaatat ctgacacaaa attcaggagc
    15421 aggaagccaa ttgacaggcg gattagcctc agttccagga gaatctgcca gaatggcgat
    15481 cgtagtcatc tcgacgttgc ctattgcgat cagctatcca tttttccaga agcattttgt
    15541 caaaggtcta acaatcggcg gagtaaaaga ataaagaaac aaaaatataa atcataaaat
    15601 taggaggaag aaaaatgaag ttatggaaga aactagcctt tacaggtgtt tcagcgttgt
    15661 tgttgggaac attagcagca tgcggaggtt ctggttctaa agaccaggca gaagctagca
    15721 attcggacac tttgcagatg tatcaaatcg gagataaacc agataatttc gatcaattga
    15781 tggaagtagc aaacaaacga atcgaaaaag agatcggtgt aaaagtcaat atcaattaca
    15841 ttggctgggg cgattacgaa aagaaaatga acgtcatcat ttcttccggg gaaaattatg
    15901 atatcgcctt tgcgaacaac tatgtttcca atgcccaaaa aggagccttt gctgatttga
    15961 ctgaattagc gcctaagtac gctgaaaaag cttataatga tttagatgaa gcatatatta
    16021 aaggaaatct ggtcaatgga aaattatatg ctttcccagt caatggaaat gtctttgctc
    16081 aacaagtact tacttttaac aaagctttac tagataaata cgatctttca attgatggaa
    16141 tccaatctta cgctgatgca gaaaaagtct tgaaggaatt ccatgaaaaa gaaccaaaca
    16201 cggcagcttt tgccattgga caaggcttca aagtccaagg gaactttgat tatcctttag
    16261 ggaatacgtt accatttgct attgatctgg atggagatga ttcgaaaatc atcaaccaat
    16321 acgataatga aacgtatatc ggactgttac gtacaatgca tagctggtac aaacaaggac
    16381 tcattccaag tgacgctgca acgagcaata aggattatcc actagaagga aatacttggc
    16441 taatgcgtgg agaaacacaa ggaccgtatg attatggcga tacaattttg acaaatgcag
    16501 caaaacaaga acttgtctca aaagcaatta ctgttccact aaaatctaca gctcaagcac
    16561 aaatggcaaa ttttgtcgta tcaaatactt caaaaaataa agaaaaatca gtagaacttc
    16621 tagggctgct taacagtgat ccagaattat tgaatgggct tgtttggggg atcgagggag
    16681 aagcatggga aaaagtcgat ggttcagatg ataaaatcaa attgctagat ggttatcagc
    16741 caaatacaca tatgtctgct tggaatacag gaaacaacaa gattctatat acacaagagt
    16801 ctattacgga tgacatgatt gccaaacgtg atcaatcgat tgctgatgca gaaacttcac
    16861 cgattttagg attcagcttc aacactgaca gtgtcaaaac agaattaagt aatatttcta
    16921 atgtcatgaa tcaatattta gatggattga atacagggac tgttgatcca gatgaaacat
    16981 taccgaaatt gaaagacgct ttggacaagg ctggctatga taaggtattg aaagaaatgc
    17041 aaaaacaata tgatgagttc cgacaagaat aataatgaat aaagacaaag gggttgtgac
    17101 aaaagacgtg tcacaacctc ttgttccgaa taaacggtgt tcaaaagcca gtttctcggt
    17161 attaagctaa atatatgtaa aaatgtggaa agccatattc acaaaatttt tcttactact
    17221 cgaaactaag cggctatttc acaaactctt ggtaaacggt gttcaatcag ctaaccctcg
    17281 gtattcgctc acaaaacgac aaaatatgag gaactatttt tccgtttgtt tctctaataa
    17341 tcaggtcaag gtgctgattg cccaaccttt ttttgtataa tgagaaagta taaacttaat
    17401 gtttggagga gaagatatgt tagggaaggt tttggaagag ttatttatcc ggatctgggt
    17461 cattataaaa ctaaccttgt atttctggat atatacattt gccggcggga tcatctttgg
    17521 attaggtgcg gcatggaaaa cagtcaatga actattttat ttgtacggat tcgagtataa
    17581 agaaatcaca ataaaaagag gctggaatat atataaaaga aattttttaa gaggcaatct
    17641 gctatttagt ttatttttat ctggtactgc tttattgagt tacaatttat atttatctgt
    17701 tcaaatcaaa gggctcattt ttttagctat tgattttatt ttactttttg ccttgctctg
    17761 cctatttgct acttatcaat acagtatgat tttagatagt gaatatacga tctctgttcc
    17821 taatctgcta aagctaagtt ttatttcggt tttttccagc ttttcgtctt ttctaaaaat
    17881 aattattggt agcggtataa ttttaggcgt gacttggcaa ttcaaaggat tgatcctgtt
    17941 tggtgtcatt ggtcttttga cggtttggaa tggtacgatg acaacacatt ggcgtgaaga
    18001 attagataaa cagttggaaa gttatgaata agtttcggat tctattttca aaaggaacct
    18061 tactaaatcg tctgttgcgg aaatacgcgg ttatcatgat cggtgtaacg atgactgcta
    18121 ccgttatttt tagtatccat acttgggaac aaaatcaaaa acaggcagaa aacatgacaa
    18181 gtgatgccgt tcaaagcaca agcagaatgc tgaatgataa aacaaccttg agtaggatca
    18241 tcaaaaacca gcttgtaggg aattccgaaa agatcgaaaa tgtgacgacc tacttaacaa
    18301 aacccataga tcagtatctg atgtatgtat acgaacagca aaacagtacc gatgaattag
    18361 tttctttccc aaatcaaatc aaagatctct acgcaaatta tgaagaacta agtgcgatat
    18421 atatcgtgtt gaatcaacta ccggagtatt atgaatcttc tagagaaaac aaaggtgggg
    18481 aaataaaaaa ggggacacca aaattactag acgcctttta tatcaagttg cctatcacac
    18541 aaggaggagc tgagtcagga agtattttta ttggttttca aaagaaagaa ctagatatga
    18601 ttttagaaaa tctaacgtca ttcaatggac tctctttata catgatttct ggaacgacaa
    18661 atcgactata cacattccat gataataaaa tagctaaaga aacgtttgaa agacaagaaa
    18721 tgatcatcac agagagtctg aagaaaaatt cacaattgcc gattgacgat ctggaaaaaa
    18781 acaattttgt tcaacaccaa gaactgtctg atgattatcg tatattagct gtactagata
    18841 aaaaaagtgt aaaaatcgaa acgggaaaaa atcttgcccc gctgatcatt ggtatcatta
    18901 ttttagacaa tatactgctt atctttcttt ataaaacatt caaacgatat tcacagcaag
    18961 tatctatcgt catgcaggcc atggatcaaa cagcagcagg gaatttagag acgcggatcg
    19021 acataacaaa cacggaatat gaactgaagg aactttcaat cggtatcaac gaaatgttgg
    19081 atagcatcaa tcagtttgta gaagacatct acaaattaga aatcaaacag caagacgcac
    19141 atatgcgggc gcttcaagca cagatcaatc cacacttttt atataataca ttagaatata
    19201 tccgaatgta tgcaatcagc gaaggaagtg aagaattagc agatgttgtg tatgcgtttt
    19261 ccgctttgct tcgaaacaat acgaatcaag agaaaacgat cactttaaaa gaagaactgg
    19321 acttttgcga aaaatatgtc tatttgtatc aaatgcgtta tcctaatcga gtagcctatc
    19381 attttatgat cgatcctgat ttggaaaaga tcgaagtacc taaatttgtg atccagccat
    19441 tagttgaaaa ttattttaaa cacggtattg attttactcg attcgataat gcgctaagtg
    19501 tcaaagtctt gcaggaaggc aaacgggtaa ggatcatcat caaagataat ggaaaaggga
    19561 tgactgaaaa acggctaaaa cagattgaag aaaaactgtc tcatcctaaa gtggaacttc
    19621 acggttctat cggtctgcaa aacgtgaatg aacgtttacg tgccagcttc ggcagcagtt
    19681 attatatgtc gcttgagaat aatgagacag gtggactgac cgtttctatt acattcaagg
    19741 agggatgagc gtgtatcaag tgttattggt agatgatgaa tacatgattt tgaatgggtt
    19801 aaaaaagata attgattggc aaagtttagg ctttcaaata gtagcaacag cagagaatgc
    19861 caaagaagga ctagctgtac ttgaacagcg acagattgat ttagtagtaa cggatgtgac
    19921 aatgccagaa ataaatggac tggaatttat tgaagcggct caaaaagaac gtcataattt
    19981 tgaatttatg attttatcag gataccaaga attcgattat ttgaaaggag gtatgcaatt
    20041 aggcgccgtg aattatttga tgaaacccgt gaataagttc gaattggttg aaagcctcaa
    20101 gaaaatcaaa acacggttag atcagcaaaa tgaacaaaaa aatcaacaag aaatctatca
    20161 agaactgtta ttcagtcaat ggttgaatga agaattggat gaaataagtg aagaagaaat
    20221 aataggacaa ataagtgaga agcagcatag gatagtattg attcaactgg caagaactca
    20281 tgatactctt atcaatagat ggttgcaaga acggcaacaa atcttttact atcaacgaaa
    20341 ttacggagac cgtgtattga tcacgttgtt gttggaggca gatgaagttg atggcttctg
    20401 ttgttttgtt caagaaaact ttcctgatca ggaatggctg atcagtatcg gggaagagac
    20461 agattctttg gacaaaatcc ctgaaacatt ccaacaagcg aaagacaatc tacagttgca
    20521 tcaattttat ggagataaag acgagcatgt tttttacgca gtacaagcag aaacaaaaga
    20581 tcaaaccatc gatttttcaa gtttcagacg cgtgctgcaa aataaacaat tggaagtcgc
    20641 tcaaaaaatg atcactgatt tttttgaaca gtttcagtta gcagccatga tgccggaaga
    20701 tatacgatat tccagttttt tactgtttat ggagattcaa cgggaattga ttgatctgga
    20761 agatgaggaa tatttacaag gaatcgaaaa aatccatcag gcaaaaacgg tgcaagaact
    20821 tcagcagcta cttctgtcat tcatcaaaaa gcatcagaga cagaaaaagt acagtgacaa
    20881 tgtggaaaaa gtaatcgtaa tcctgcacca gcattatcaa gaaccactta cactcaaaga
    20941 agtgtcagaa aacctgcatt taaatgtcat gtatctagga cagctgttca aaaaagaaac
    21001 gaaaaaaagt ttttcagctt atttgaacca tttacgtatg gaaaaagcga aacaactgct
    21061 tttgcacagt aatcaaaaca tcaatgagat agcaagtgag attggctaca acaacaccac
    21121 gtatttctct aaattattca agaaaatcgt cggcagatcc cctaaagagt accgagaaaa
    21181 cagtggagaa tcataaaaga aaaagccaat gtaatgaaat aacttataaa aaattgtcta
    21241 gaaatctaat tttgggctgt tcttattact tgaggcaagg cagctttttc atgacctctc
    21301 gataaacgga gttcaaaagc cagtttctcg gta
//
LOCUS       DAMEET010000049        21169 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.050, whole genome shotgun sequence.
ACCESSION   DAMEET010000049 DAMEET010000000
VERSION     DAMEET010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.050"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            342..1028
                     /locus_tag="PB780_001614"
     CDS             342..1028
                     /locus_tag="PB780_001614"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDE0733670.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            1048..4629
                     /gene="smc"
                     /locus_tag="PB780_001615"
     CDS             1048..4629
                     /gene="smc"
                     /locus_tag="PB780_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDE0733671.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            4638..5447
                     /locus_tag="PB780_001616"
     CDS             4638..5447
                     /locus_tag="PB780_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDE0733672.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            5458..6456
                     /gene="ftsY"
                     /locus_tag="PB780_001617"
     CDS             5458..6456
                     /gene="ftsY"
                     /locus_tag="PB780_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDE0733673.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            6643..8910
                     /gene="gshAB"
                     /locus_tag="PB780_001618"
     CDS             6643..8910
                     /gene="gshAB"
                     /locus_tag="PB780_001618"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HDE0733674.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(8999..9451)
                     /locus_tag="PB780_001619"
     CDS             complement(8999..9451)
                     /locus_tag="PB780_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HDE0733675.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            9601..10551
                     /locus_tag="PB780_001620"
     CDS             9601..10551
                     /locus_tag="PB780_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDE0733676.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            10544..11503
                     /locus_tag="PB780_001621"
     CDS             10544..11503
                     /locus_tag="PB780_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDE0733677.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            11500..12255
                     /locus_tag="PB780_001622"
     CDS             11500..12255
                     /locus_tag="PB780_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDE0733678.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            12278..13231
                     /locus_tag="PB780_001623"
     CDS             12278..13231
                     /locus_tag="PB780_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDE0733679.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(13875..14171)
                     /locus_tag="PB780_001624"
     CDS             complement(13875..14171)
                     /locus_tag="PB780_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HDE0733680.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            14526..14882
                     /locus_tag="PB780_001625"
     CDS             14526..14882
                     /locus_tag="PB780_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDE0733681.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            14892..15791
                     /locus_tag="PB780_001626"
     CDS             14892..15791
                     /locus_tag="PB780_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HDE0733682.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            16308..17039
                     /locus_tag="PB780_001627"
     CDS             16308..17039
                     /locus_tag="PB780_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDE0733683.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            17184..19211
                     /locus_tag="PB780_001628"
     CDS             17184..19211
                     /locus_tag="PB780_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HDE0733684.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            19319..20416
                     /locus_tag="PB780_001629"
     CDS             19319..20416
                     /locus_tag="PB780_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDE0733685.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            20723..>21169
                     /locus_tag="PB780_001630"
     CDS             20723..>21169
                     /locus_tag="PB780_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDE0733686.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT         7169 a         3590 c         4427 g         5983 t
ORIGIN      
        1 aaaatgaccg aaaaagggcg atgtcctttt tcggtttttt gatatttttc tgtttatttc
       61 tctaatattc aggtcaaggc gctgatttca tgacttctcc aataaacggt gttcaaagtc
      121 cagtttctcg gtattaagtc aaatttatgc aaaaatatgg aaagccattt tcacaaaatt
      181 ttttcttcat actcgaaact aaacgaattt ttcacaactt ctcgtcaatc actaggatta
      241 ttaaagcttt acttaccttt tgaccgaacc atttcaaatt tgacaaaaaa ccagtataat
      301 aaggaagtat cgaaaaaata tccataaaag gaatgtggaa aatggacaac cagctgacaa
      361 aagaactaaa agaaaactat aacatcgttt ttcacgatct aaatttgctt gaacaagctt
      421 ttacccattc atcctatgtg aatgagcatc gcaatctgca attatcggat aatgagcgac
      481 ttgaattttt aggagatgcc gttttagaac tgatggtttc tcagtattta ttccgtttat
      541 atcctcattt gccagaagga aaattgacaa aaacacgagc tgcgatcgtt cgcgaagaca
      601 gcttgtcgaa atttgccaaa gaatgtcact ttgatcaata tgttttgttg ggcaagggag
      661 aagagaattc aggaggacgt acgcgaccag cattgctttg cgacctattc gaagcttttt
      721 taggcgcgtt atatcttgac cagggattcg acacgaccca tgcattcatc gaaaaagtga
      781 tttttcctaa agtcaaagca ggtgcttttt cacatgagat ggatcataaa acaaaactac
      841 aagaagtctt gcaaaaatca ggtgatgtat caatcgaata ccgattgatc aatgaagaag
      901 gtccagctca tgaacgagta ttttggatcg aagtttatgt agatgatcaa ttgattggca
      961 ctggacaagg gaaatcgaaa aaattagcag aacaagctgc ggcggaaaat gcattggcag
     1021 cactgtaaaa gtaagtgagg gaaagctttg tatttgaaac gaattgaaat agccggtttt
     1081 aaatcatttg cagacaagac agtcattgat tttgaaaata gcgtcacagc agtggttgga
     1141 ccaaatggca gcggaaaaag caatattaca gaggctatcc gctgggtatt aggagaacaa
     1201 tctgctaaaa gtctgcgagg cggaaaaatg ccggatatca tctttgccgg ttccgatacc
     1261 cgtaagcagc tgaatattgc agaagtcaca gtgattttag acaatagtga tcattatctg
     1321 ccattggagt acaatgagat cagtgtaacc cgccgttatc gacggactgg ggaaagcgag
     1381 tttttcatca ataaacaaag ctgcagatta aaagatatcc aagaattgtt cttagattct
     1441 ggattaggaa aagaatcgtt ctcaatcatt tctcaaggaa aagtagaagc gattttcagt
     1501 agtaaaccgg aagaccgtcg tgggatcttt gaagaagcag ctggtgtctt gaaatacaag
     1561 cagcggaaaa agaaagcaga acagaagctt tttgaaacag aagacaacct cagccgcgtc
     1621 caagacatca tccacgaatt ggaagaacag ttaactcctt tggccgccca gagtgaggca
     1681 gcaaaggagt ttttacggct aaaagaaaca ctgactcaaa ccgatgtctc tttgatggtt
     1741 gctgaaatca agacagcaaa aaaagattgg gacaacaaac aagcacagct tgcgaaattc
     1801 aatctagaat taggcaaact atctgagtcg atccaagaac aagaatccat tttggcgaag
     1861 cagcgtaaag aaaatgcgca agccgatcgt ctgatcgaaa aaaatcagca agtattatta
     1921 gatctttcgg aaaaactgaa gcagacagaa ggacaaaaag atgttttgca ggagcggaca
     1981 aagcatacac aaaaaagctc gcaagaatat caaacttcat tagcagaagc acaaaaaaag
     2041 gttaaacatt ttgaaaagct tcaagaaagc ttgatgaaag cagcagcaga aaaagaaaca
     2101 gagatccaaa aagctgaagc aaatctcata aaaacacagc aagaattaga aaaataccaa
     2161 aaatcaacga aagaattatt ggcagaatta agagatcaat atgttgattt gatgcaagaa
     2221 caagcagctg taggaaacga actgaagtat ctagaacgtc aatatataca agaaacagca
     2281 aaaagcaagc agacgttagc caagcaatcg gaagtagaag catcagtaga ccgattgatc
     2341 ttacaaaaag aagaattgac acaaaagcag gcgcagctaa aatcatcttt aactgaaaca
     2401 aaagagaagt tagaaatgat ccaacaaaac gggaaaaagt tccaagaagc attagcaaaa
     2461 gaacagccta aaatgtatca attaatgaat caagtccagc agctacgtgc tcgtaagaaa
     2521 agccttcaag aaatccaaga aaactatttt ggtttttacc aaggggttcg attggtcttg
     2581 cagcataaac agcagctttc gggtattgtc ggagcagtgg ctgaactgat cgatgttcct
     2641 gcagatttca cgttagctat cgaaacagct ttaggtgggg cagcccagca tgtcatcgtt
     2701 gaaaatgaaa aagatgctcg gcaagccatc acgtatttga aacagcaaag aggaggaaga
     2761 gcaacattcc ttccactgac gacaatcaaa ccaagacaat taccggctca tatattgaca
     2821 caagcagcag ctgtagaagg gtttataggg attgccagtg aacaagtcag ttatcccgat
     2881 caaatccaaa ctgtggttca aaacctgcta ggaacgatcc ttttagccaa agacttaact
     2941 agtgccaatg caatcgcgca aacgatccgc tatcaatatc gagtggtttc cttagaaggt
     3001 gatgtgatga atgccggagg atccatgaca ggtggagcga ataaacgagg aaaccaaggc
     3061 agtttgtttg tacaaaatca agaattgaaa cagttgacta gtgaatttga agaagcagat
     3121 aaacaactcc aagcacaaga aaaaaaagtt caggagctgc aacaagaaac agctcgttta
     3181 gccgaagaac aagaagtttt gcgtacacga ggcgaacaat tacgttttga agaacaagaa
     3241 gcgacaaatc agctacaaaa catcataaat gaacttgaac gttttgaaaa agaaaagcaa
     3301 atctcaactt ttgagacacg tgaactgcag cagtttatcg aagattacga aaagcaaacg
     3361 aacgaattga aagacaaaca aacagatctt gaatcgcaac gacaaaaaat cgacgaagaa
     3421 atcaaatcgt taagtcaaga aagcgaccag atggaagcca gaagagcaca agtacaaagt
     3481 caaaaagcac aagaacaagc agatcttgct gtcttgaaag agcagtttaa tcatctgcag
     3541 atccagctcc gcggtgcccg agtccaaaaa gcagaggcaa ccgaacgtca agaagcgata
     3601 gaaaagcagt tggcgacatt gacagcagac ttttctgatc atgaagtcac agaagaaagt
     3661 ttggaaaaac aaattaacga attatctgct cagcgagaaa cattaaaagc cgagttggca
     3721 aaagccaaag agcagcgaga tagaaggcaa aaggagatcg ataagcttga agcagtcttg
     3781 gcagagcgta atcgtgagca aaaagcacga ctgtcggaac aatccaaatt agaagtccaa
     3841 aaagaccgag ctgaaatgat tttagataat catttgacct atctgcagtc agaatatcag
     3901 ctgacatttg agaaagcttc ccaagactat caagaaacaa ccgatatcga agacagtaga
     3961 acaaaggttt catccttaaa agaacagatt gaaaaactgg gaccagtaaa cttaaattca
     4021 atcgaacaat acgaacaagt cagtgaaaga catacattct tagcaacaca gcgagatgat
     4081 ctgttagcag cgaaaaacca attgtttgaa acgatggatg agatggatga tgaagtgcgg
     4141 acaagattca aagaagtatt tgaagctatc cgtcaagaat tcaaaattgt ctttccaaat
     4201 atgtttggcg gcggtaaagc tgaattagta ctgacggaac caactgattt gctgaaaaca
     4261 ggtattgaga ttgaagtaca acctcctggc aaaaaactgc aaagtctaag tttgctttct
     4321 ggaggagaac gagcacttac agccatcgcg ttgctctttt ccattatccg tgtctgtcct
     4381 gttccgtttt gtatcttaga tgaggtagaa gcagcgttag atgaagccaa cgtgaaacgt
     4441 tttggtcgtt acttgagcga tttccaagat gacacacaat ttatcgtagt cacacaccgc
     4501 aaaggaacga tggtcgcagc agatgtactg tacggagtga cgatgcagga atccggtgta
     4561 tcaaaaatcg tttctgtacg gatggaagat atcaatgaag aggcgaaaat caacgaaacg
     4621 aaagtgtagg aacgaaaatg atcaaattag tagcaatcga tttggacggt acattattaa
     4681 atgaacaaaa gctgatttca gatgagaata aacaagcatt agcacaagca aaacagcaag
     4741 gggtcaagat cgttctatgt acaggacgcc ccttagctgc gatggcgcat tatctgcaag
     4801 aattaggact ggtagatgaa ggtgactaca gcatcacttt caacggcggt ttggtacaaa
     4861 aaaatgatac tggtgaaatc atggaaaaga aagtgatgga agtatcagat atccaacgct
     4921 tatacaaact ggctcagcaa ttggatctgc cactagatgt tttgtctgac aatgtggttt
     4981 tacagctgcc tagtgcccca aggaagcaat ccttgtacaa tgtgttgaat aagctgctgc
     5041 aattccaacc agcggatata agcgatatta cagatgaatt tgtgcttaat aaggcagtaa
     5101 tcgcttatga ccaagaagaa ttagatccta aaatcaaaga aataccagca gaattccatg
     5161 aaatgtatga gatcatcaaa acaagaagta tgcttttgga atttatgcca aaaggtgtta
     5221 caaaagctta tggtatttct ttattggcaa aagatcttgg tttgaaacaa gaagaaatca
     5281 tggcgatcgg tgatgaggaa aatgatttac cgatgatcca atatgctgga ctaggcgtag
     5341 cgatggagaa tgctgtgcct ttcgtcaaag aagcagcaga tttcgtgaca gccagcaatg
     5401 aagaaaatgg cgtagccaaa gtgatccaaa aatttgtatt agagtagggg gagtcaaatg
     5461 ggattttttg ataaaatcaa gcgtgctttt actggcgaag agaaaacaga agaagttgaa
     5521 gtacaggaga agtacgacaa gggtttagaa aagacgcgta aaacatttgg tgaacggatg
     5581 aatgagttgt ttgccaattt tcgaacagtc gatgaagact tttttgaaga attagaagaa
     5641 acacttatcg gtgcagatgt aggttttgaa acagcgatac gaattaccga agcattacgt
     5701 caagaagtga aactcagaaa tgcgaaaaaa tcggcagaag tccaaaatgc gatcatcgag
     5761 aaacttgtgg atctctatga agcggaagga atcaatgaag taaatgaact aaatatccaa
     5821 gaagacggac taacagttat tttgtttgtc ggtgttaatg gtgtagggaa aacaacaagt
     5881 attggtaaac ttgcccatca gttcaaaaac gaaggtaaaa aagtattgat ggcagcagca
     5941 gataccttcc gcgctggagc aatcgaccag ttagttgtct ggggcgaacg cgctggtgtt
     6001 gaagttgttc gtggcaatgc aggaggcgat ccagcagctg tcgtattcga tgctttggaa
     6061 agagcaaaga cagagcaggc agatgtttta ctagttgata ctgcaggccg actgcaaaat
     6121 aaagtgaact tgatgaacga attagaaaaa ataaaacgag taatccagcg ggaattacca
     6181 gaagcacccc aagaaacact tcttgtatta gatgcaacca ctggtcaaaa cgcgatggtt
     6241 caagcaaaac aattcaaaga aacaacagat gtgaccggtc ttgttttgac aaaattagat
     6301 ggtacagcaa agggcggtat cgtacttgct atccgtaatg aattgcattt accagtgaaa
     6361 ttggtcggtc ttggtgaagg aatcgatgac ttggagccgt ttgatccaaa tgattttgtc
     6421 gttggattgt tcaaaggatt actaaaagaa gaataaccca tgtctaaaaa gcaggagctg
     6481 tgacaaaagt tgcagctcct tgtttgaatg attgttttta acatagctgt gtttttgatg
     6541 ccttcatttt tatacttatt tgtaagcggt tctcaaacaa tttcgttctt tttcttagac
     6601 tttttgatta taattagatt catatgaatt ggaggtcact taatgatgaa ttttaagcaa
     6661 ttattattgc atgtcaatgc gcgccctttt atcgaccaag ctcgatttgg tatagaacgg
     6721 gaaggacagc gggttgatct tgcaggaaat ctagcaaaaa ccgatcatcc agcaatcttt
     6781 ggcgatcgat cctatcaccc ctatatccaa acagatttta gcgaaacaca aacagagatg
     6841 atcacccctg ttaccgattc tattcccgaa ttatttcagt atctcgctgc tgtttatgat
     6901 gtgactgctc gttctatacc gaaagaagag atgatctggc cattaagtat gccacctgcc
     6961 ttaccggaaa aagacgaaga gatcattatt gcaaaattaa aaaatttcga agatgtcttg
     7021 tatcgacgtt atttagcaaa agaatacggg aagcgaaaac agatggtaag cggtattcat
     7081 ttcaattttg aatttggcga tgagttgcta agaacattgt tcagccatca ggaagaattt
     7141 caagattttt ccgaattcaa aacagaactt tatttgaaaa cagcgagaaa ctttatgcgc
     7201 tatcgctgga tgatcactta tttattcggt gcttcaccaa tgagtgagaa gaactacttt
     7261 ttagatgaat cacatccgca agaacctgtt cgcagtattc gcaacagtgc gctgggctat
     7321 acgaatcatc caaatgtgaa agtttcctat gcttctatga aacagtattt agcagatatc
     7381 gagcgaatga tcgaagaagg gaaactttcg gaggagaaag aattttatac accgcttcgt
     7441 ttccgaggcg gaaagaaagt cgcagattta gcaacaacag gtgttcgtta tattgaattg
     7501 cggaatatcg atttgaatcc ttatgcaaga ttgggtatca atccagagca agttcggttc
     7561 ttacaattat tcctgatgta catgttatgg acagaagaaa aagaagactg tgaccaatgg
     7621 gtagcagaag gtacgactcg aaataacaaa gtagcactag aacaaccatc agatcaaaca
     7681 gaatttcatc aagaaggtag agaaattctt gaaggaatga agcaaatgct ggttgaattg
     7741 gattggttgg attctcttta tttagtagag gaagcattga ctcaaatgga tcatcctgaa
     7801 caaacgttag cagcgaaact ctatcaagaa gcacagctat cgagtcagca agaagttgct
     7861 gtggcattgg gacatcaata ttataaagaa agccatgaac gtccctatca gttggccggt
     7921 tttcgcgaaa tggaactttc tactcagata tttatgttcg atgccatcca aaaaggtgtc
     7981 caagtcaaag tattagatga gtcggatcaa tttttgcgtc tgcaattcca agaccatgta
     8041 gaatatgtga aaaatgcaaa catgacaagt aaggacagct atatcgtccc acttatcatg
     8101 gaaaacaaaa cagtaacgaa aaaagtctta aaagaagctg ggttccgggt accaggaggc
     8161 gcagaatttt catccatgga agaagcggta aaagcttatc cgagatttgc cgagcaggca
     8221 tttgtgatca aacctaaatc aaccaattac gggttaggta tcacgatttt caaggaagga
     8281 gctagtctgg aagattatca agcaggttta gccattgctt ttcgtgaaga cagttcggtt
     8341 ttagtagaag aatttatgcc gggaacggaa tatcgtttct ttgtgatcga cggagaagtc
     8401 caagccatca tgttgcgagt ccctgctaat gttattggtg acagtatccg aacagtgaaa
     8461 gaacttgttg aagaaaaaaa tagtgaccct ttgcgtggaa cgaatcatcg tgcaccttta
     8521 gaattgatcc aattaggtga attagaacaa ttgatgttga aagaacaagg gttgacgatc
     8581 gaatcagttc cccaagccaa tcaaattgtt tatctaaggg agaactcaaa tatcagtaca
     8641 ggtggtgatt cgatcgatat gactgatgaa ttttctgaag cttacaaaaa aatcgccgtt
     8701 tctgcggtag aagcactagg agctaagatt agtggtatcg atttgattat accagataaa
     8761 gaaatcgatc ctacaactga taaaaaagca tatgggatca tcgaagcaaa tttcaatcca
     8821 gccatgcata tgcatgttta tccttttgca ggaaaaggca gaagattgac gatgaatgta
     8881 ttgaaattgt tatatcctga agtattttaa aagaaaaagc ttttgacaga ttgtcctatt
     8941 tgatgtcaat ctgatcaaaa gctttttttg catgtaaaaa tttatgtgtt ttttcgtttt
     9001 aaaataaacg atcccagaag ctttttttct tttttggttt ttctttttca gctggcgaag
     9061 agttttcttc tgggtatttt ttttcgatat caatcacagg ttcatttact gcttcttttg
     9121 ctgttaagag caatccgaat tcctcaggat ttgcagtgac gtaatcgttg actacagtga
     9181 aacggctatt gtttttcgtt gctagctgga tatattggtt ttggacagaa aggggtagag
     9241 ccccattcaa gagtatggaa gcatctggat gttttttgag ttcttttaaa aagttttctt
     9301 tgttttttgg ttggatcatt tcagcaacag tcatactaag atagcagcgt tccctgaatg
     9361 tccctaaata tttatgctgt tcttcagggt tcactaaagg tgtaccataa agtcccttat
     9421 ccaaatggct ttttacatca tcttgtgcca taaaaaatac cctccttctt ctctttgtct
     9481 ttcttacgat tatagcatat tcccaataat acctaaaaca tatgaaaaag cggttgcttt
     9541 tgttcaacga ttcacttaaa ataagaatga aaatcattct caattagaaa ggtggattgg
     9601 atgcgctacg gacgactagt agcattatta gttattttaa gtagtatttc attgttcatc
     9661 ggagtatcga atgtacaatt gtcagaactg ctccagttag atgggcaaag cttacaaatc
     9721 ttgctagtga ctcgtttgcc acgaacgatt tgtttgatcc ttgtgggagc gaccagtagt
     9781 atttgtgggt tgatcatgca acatttgacc caaaataaat ttgtttcacc aacgactgcg
     9841 ggcacgatgg atagtgcgcg tttaggtatt ttagtggcta tgattttcct acctggtgca
     9901 tccttgattg tccgatcctt gacagcattt tgctttgctt ttgcaggaac attgcttttt
     9961 ttatccctta cgcgattgtt cctccagaaa aatcaattga tgcttccgtt gattggagtc
    10021 atgttaggga atatcattgg ttcagttgct actttttttg cttatcaatt ccagttggta
    10081 caaaatatga cttcatggct tcaagggaat tttgcgactg tgatacgagg aaattatgag
    10141 ttattgtatg ctgttgttcc attgttgctc ttggtttggc tatttgctta ccgatttacg
    10201 atcattggtc tgggcgaatc gtttgcagta aatctgggaa tagattacca gaagatgcag
    10261 tttttaggca ttggcttagt atcattggct agtgcagtca tgttgatcat ggtaggaagc
    10321 attcctttta tcggagtgat cattccgaat cttgtctcta gaatatatgg agatcaagtg
    10381 cacaaaacaa ttggcgtaac tgctttagca ggtagtttgt tcttagtatt ttgcgatctt
    10441 gtcgctcgcg tgttgatttt tccttatgaa ataccagtca gtgtgatcgt tggaatcatc
    10501 ggtggggcta tcttccttta tcttctgata aggggacgac gatatgctta aaaaaaagcc
    10561 atctattttt attggtatgc ttgttcttgt tttatttgtt acaggaatgt acttgacgat
    10621 ccatacatat ggaaactggc attttgcatg tgcattgagg agtaagaaaa tttttgcatt
    10681 tatccttgtc tcgctagctg tcagcagtgc cacaatcagc tttcagacaa ttacgcataa
    10741 ccagtttttg actccttcag tattgggact tgagtcgtta tatgtccttg tccaaacggt
    10801 ccttttcttc tttatcggag gagtgacgat gcttcagcag acaggtacag tttttttctt
    10861 attgaacgtt gggctgatgg tcgggctgag tctattgtta tttttctttc ttttgaaaaa
    10921 aatgagagcg aatctttttt tattactgat gtcagggatg attttaggta cgctttttgg
    10981 tagtgtcagt acctttttac aagtcatcat ggatccgaat gaatatgatt tgcttcaagg
    11041 cagattattt gcaagctttg gcaatatcca gagtcagtat cttttgcctg cttctttgat
    11101 cattatcggc tgctggtacc tgttatttcg atatcgacat gtgctagacg ttttccatct
    11161 cggaaatgaa caagcagtaa acctcgggat agacacagat cgttttgaat ttttgatttt
    11221 atgttgtgtc agtcttttga cagggacatc aacggctctt gtgggtccag ttactttttt
    11281 agggtttatt gtcgcaaata tcagttatcg tgtcttttct acttatcgtc atggtcagct
    11341 atttatcgga agctttctgg tagctatgct gtttctgaca ggaggacagc tcgcagtaga
    11401 gcagattttc ggctggaata caacagtgag tgtcgtcgtt gaatttgtcg gaggaattta
    11461 ttttgtcaca aaattgattc gcgaaaggaa gagtatccta tgattgattt acaaaaggtc
    11521 aataaactgt accatggaaa atcagtggtt aaagatgtac agttaacgat tcctgaaaag
    11581 aaattgactg cttttatcgg tccaaatggt gctggaaagt caacggtgtt gtcgatgatc
    11641 agtcgtttga taccaaaaga tacaggtgaa atctatttag accataacga agtaaaggct
    11701 tggaaatcca atgaattagc caaaaaatta tcgatcctac gacaatcgaa tgaaataaat
    11761 gtccaaatca ccgtgaaaga attagtagaa tttggacgat ttccttatac gaaaggaaaa
    11821 ttgacgacaa aagacgagga aatcgttcag gaatcactgg aacaccttgg gttagaagaa
    11881 atagcggatc aatcgattga cactctttca ggcggtcaac tccaacgtgc gtatattgca
    11941 atggtgctag cgcaagacac ggattatatt ttactggatg aacctttgaa caatctagat
    12001 atgaattttg cggttcaaat catgcagatt ttaaaaaatt tagtacaaga aatcgggaaa
    12061 acaattgtta ttgttcttca tgatatcaac tttgctgcaa gttatgcaga cgaaattgtt
    12121 gcaatgaaag atggacagtt atttacgcaa ggaacgaccc aagaaatgat aaaaaaagat
    12181 gtattggatg aattatacga aatggacata cggatttgtg agattgaagg ccgcagattc
    12241 tgtctttatt tctaaaacta acattaggag agaagagatg aaaaaattag tagtaggtat
    12301 cttgattcta gcatcatttg gtttagcagc atgtggaaat agcaacaaca ccagtcaggc
    12361 agacacaaaa aaaagcagca cacaaactga aactacattg acgataacag atagtaatgg
    12421 agaccaaatc gaggtgccaa ataatccaga aaaagtcgtc gtattcgaca atggttcttt
    12481 agatacgatg gatgctcttg gtgttggaga taaggtagtt ggagcagcta caagcagcct
    12541 tcctgagtat ctttcatcct ataaaaaagt agaatcagca ggtggaataa aagaaccaga
    12601 cttagaaaaa attaatcagc ttcagccaga tctaatcatc atttctggcc gacaaagaga
    12661 ttttcaaaaa gatctaagtc aaattgctcc gaccattttc ctttctttag atgcgaaaaa
    12721 tccatgggaa tctttccagc aaaatgtcac tgctttaggg gagatctttg gtaagcaaga
    12781 agaagctaaa actcaattag aggaattatc tagtgcaatc gatcagacga aaaagaaagc
    12841 agaagctact gacaaaaaag cactagtcac acttgtgaat gaagggcaat tatcagccta
    12901 cggatctggt tctagattcg gtttcatcca tgatttattt ggctttgaac aagcagatga
    12961 tcaaatcgaa gcatccactc acggtcaaag tgtttcttat gaatatgtgc tggaaaaaaa
    13021 tccggatatc ttatttgtag tagacagaac aaaagctatt ggtggagacg acagcaaaga
    13081 tgatatttct gccaatgaac tggtagcaca aacgaatgct ggtaaaaatc agcagatcat
    13141 ctcccttgaa ccagatgtct ggtatttaag cgggggcggt ttagaatcaa tgaaactgat
    13201 gatcgaagat gtgaatcaag catttaaata gcataaataa cagaggatta aactctcata
    13261 ctatgtaaca ataatgtttc ttacattctt aaatagcaag agcctatcct caaagaagtt
    13321 atcaaaaacc atccgtaaaa tcgtattgat gattttacgg atggttttta tttatttgag
    13381 aaatcttttt tcaacgtgaa ttcattatct acatcatacg ggttgattct agaatgggga
    13441 gtggcaaaaa atagtgtgat ccattttttc agcatcaaga tcgcctcctt tgatttgaat
    13501 caaaggtaca aagcgaaact tagataaaac ttaaatttag atatttttat tttgtaccga
    13561 tctcttcttt aaacgatgtt ccatctgctg cttctcggca ataagtccac tagactagaa
    13621 aatatgagaa gctattttct gtccagcgtt cttattactc aaagcaaatc gcagttgtca
    13681 caatctcttc taaacgatgt tccatctgct gcttctcggc aataagccca ctagactaga
    13741 aaatatgaga agctattttc tgtccagcgt tcttattact caaagcaaat cgcagttgtc
    13801 acaatctctt ctaaacaatg ttcaatcagc tgaccttcgg tattagctca aagcactgat
    13861 ttcacaacct ctatttaggc atgtgtctca gcaagcggtt gatagttgat gatctgcatt
    13921 tttcgataaa aatcggcggg aagcttactc caccgtttta ataattgata gaaataactc
    13981 tcttctgctt tgattttata ctcgcttaat atcaatccaa aactattcgt tttttttgaa
    14041 gctaattttt tataaatatc caatgctttg gttttatctt cattgacaaa gagtgttttt
    14101 acttcaatat aagaaccctc gatattttgt gtttcttcga taaaatagat gttttttata
    14161 tattccttca tagtgattcc tccaaagaag tcttttattt gataagttta ctctaccgaa
    14221 gagttgttaa taatccgtga acaaaaagta attctttttc caatagttgt taaaggtttt
    14281 ttatttttta tagtattttt tgttatttaa gaaaggaata aggttaaaca aataacaggt
    14341 tgctttttgg gtctttttcc gttcaagaaa ctgaacgaag aaaagggaaa gttgtctctt
    14401 ttgtgataag agaaatcata ataaacgaat gacttacttt ccaataaatt gagaacagtc
    14461 agaaccagac aaattagttg catctgatga ataaagtgat tattctatga catgaggtga
    14521 tacagatgaa attagaagtg acaaaagaag cacaagaagt attgaagaat aaattagaag
    14581 cagacgatca acttttatta gatatcgaaa acggtgacgg tccatttgca gaaagccaag
    14641 tcagttgcca attggatacg gcatttcgat tgatcattgt caaaaaagat acacaaacag
    14701 atctaagtct ttattctgta gtggctgata caccagttgg accaatcaaa atcaaagaca
    14761 gtgcaatact ttacatggat gatccatcca cacttgcttt agaaccaact tataatagtt
    14821 tacaattaaa aggaccaagc ggcttgagaa aaggcaactt gcaagttgtt cgaaaagact
    14881 agagggataa aatgggaaaa ttggcgatca tcagcgacct acatgtggac atcaataaat
    14941 tcggcgaatc agagctgatg cagctttggc aagttttgca aaagcacaag atcactcggc
    15001 ttcatctagc cggagatacg gctaataaag tagaccgttg cctagctgtt gtcgaatttt
    15061 tcgtcaataa aggattatct acgacatttc atttcggcaa tcacgagttg gcagatgtgc
    15121 ccaatgaaca gatgatggac cattttccag acccacattt tttaaatgaa cggtatattg
    15181 ctttaaatga gcagaccgtt cttttaggga tgaatggctg gtacgactat cagttttccg
    15241 aattgaaaga ttacgaggca atcgtacgaa tgaagcgttt gtattggtat gatcggttca
    15301 tccagcgtgg gaaaacagat ctcgaggtca atgcagaaat gcttgcagct gcaaaaaatt
    15361 tgctagacca gctggaagca aaaaaacttg aggtgatttt atccactcat ttcgttccta
    15421 aacgggcatt tatcatgtat caatctgctc cttatgagcg ttggaacaaa ttaaatgcat
    15481 ttttaggatc agaatcattc ggagaattac ttgaccgtta ttcaaatatc aaacaagtag
    15541 tttttggcca tacgcatcgt cgctttgaag ataaatccat cggtcacacg atttacagct
    15601 gcagaccttt tggttattat tatgaatggc agctgacccg agattttgtc ttgaaagaac
    15661 agttgctaga cagctatgat cctatgaaat tacgtgtcct cttgcgtgcc catcacccag
    15721 cttttcaggc ctatcaaaca caacatttgc aagaggaatt tgaacaagcg atgacaatcg
    15781 ttccgtattg agacagcatt agtaatgtgt aaaagaaaat accttgtaaa acataaggat
    15841 aagtaagagc agtagatcca tttatcgtgg atatgctgct ttttttgcgg aaaataatca
    15901 gcgaatgttt atttgatcgg atgatttgta actcgcagag tactagatgc aagcgtgtat
    15961 tctttttttg aacgataatc acaaaaaaaa atataatata cattttttat ttaattataa
    16021 ttaatattga ttgaagtcta ggcagtcaag tcacattttg atagatagga gggggactta
    16081 cgaattattt taaaggctat gacagctgta tttctagatt gtgtttgcat caagttgcaa
    16141 taaaaactag aaatcaaatc gggctgataa tcatttataa ctaatcgata cataggacca
    16201 aatataggtc tatgttcttt tatttcataa gtatcgtaaa acagaatttt gctatggatt
    16261 aatccaatcg actgatatgt ttagtaaata aagaaagagg aatgtcaatg atgaaaaaag
    16321 aaatcgtatt aacagcagca atgatgttat tcagcatggt agcaagtacg acttttgttt
    16381 ctgcaactga agtataccca aaggaatata acacagaagg aacgattaca tttgaagcag
    16441 gagatgaggg tgtaactcct cctgttgatc cagagaatcc tgatcccaat aaacctgtcg
    16501 atccatcaga tcctccaagc cctggaacag gaggggcatt atcgattgat tatggatcaa
    16561 aattcaaatt tgggactcaa aaaatttcta ctgcggataa aacgtattat gcagctgctg
    16621 atgtgatgaa cgatgggtct agaaaaccga catatgtcca agtgacagat aggagaagta
    16681 ccttatctgg gtggaaatta agtgtctctc aaccagaaca gtttaaaact gccagtgggg
    16741 atgaactagt tggagcacag ctgaaattta cgaaagggca ggctgtttct ttggttgatc
    16801 caacatatac gccgcaaacg gtgaattctg aattaacgtt gacaccggga ggaaacaata
    16861 cgttagcaat taatgctaaa tcaggaacag gtgtggggac ttgggtctat cgatttggtg
    16921 caaatgaaaa tgaaaatcaa gatgcggtac agctttcggt acccggtaaa tctgtcaagt
    16981 tagcgcaaca atattccact aaattagttt ggacattaga agatacacct aacaactagt
    17041 ttactgaaaa gaactgttaa aaataaatac gtagtcatat tgtcttgcga ggtaatcgag
    17101 caaaaattgt caaaggctag gctaaatttg tttggatacg aatagttcat ctaggaattt
    17161 aacgatcagg aggaaaagat atattgaaaa agaggaggat cgtgctcatt ttaagcagtc
    17221 tcttagtgac taatatcttt ccgatcatag ggtactcttt ggaattgaat cagttggagc
    17281 atggaaatag aagagacgaa gaaagcaaac tcacttcgac attcgacagt ttaatagaat
    17341 ccaaccagac acaagaaagc agtgaggatt taagtatttc gagtagtgga gagaccactg
    17401 acaacgtaga agctgagggt tcggacaaac aaggactaga agatgcacca atttctgcgg
    17461 ttgagctgcc tgacgatccc aatattattt ctatcgataa aatttttcaa acacctatcg
    17521 gtactagcac gtccatattg gaaaatggga agttactgca gttgaaccca gcagaaaggt
    17581 ctcaaagagg agctatttgg tcaaataaga agattagttt actctcggat tttacattta
    17641 aatcttattt atacttagga aatcaacgag atagagcggg agatggcatg acctttacat
    17701 tgactggcga tcctagaatg gctagtaatc ccagtcaagt tattggatcg ccaggtatgg
    17761 gaattggctc atattctact cgttctggcc aaccttacat tgcgaatgcg ctctcgattg
    17821 agtttgatac gtataaaaat agtggttctt ctgatcgaat ggatcgagag ataactgctg
    17881 ataatagagg gcatggacat gtcgcttttg tcacgcctaa accaaataat aataattatt
    17941 ctggagagca tactggcgtg acggtagctc ctacatttct ttctaatggc acatggcgct
    18001 tgttaataat taaatgggac gcggagcaaa gaagtcttac ttatgatttg gaaggtgtcg
    18061 gtcactcttc tcggattata cctgatctca ttaggcagtt tggttctaca gaagtaaatt
    18121 gggggtttac ttcttcaaca ggtacttttt ttcaagaaaa tgctgtcgct gtcacgcgac
    18181 ttccatcgaa tgtcacatca acagctgatg taaaagtcaa cgatagtgat tatggggcca
    18241 tcgctgaagc tggaggaaac gataagataa ctatcagaaa tacattgaag gtcgaggggg
    18301 attttcttga atataagaaa atgcagacta gtatcgacct cccacaagaa ttaactattc
    18361 ccgagcatat cttgatcaat ggaaatcaaa ttgatgccaa agatatcttg ataaccggaa
    18421 atcgagtcac gctaaatctg acagattatt ttattgctgg agatacgtta ataatcgaat
    18481 tggagacaac tttgcaggaa gtaattcctg aaaaagtact cactatgcat tttgaatacc
    18541 ttgaaaacgg gaatttgatt gagcaatcga atgaaataaa cctaaagatc gcaaagctga
    18601 aggagataga tatccatgtt tattataaag ataaagacag tcagattgat attgctgaaa
    18661 tgaaaacaat cactgggaaa ataggagaga agtataaaga aactcctatt gatattgaag
    18721 ggtatgtttt tgactcggat tcagcgaata cagaaggcat cttctccgaa aatacagagg
    18781 atatctattt ttattacaga aaaggcaaac tttatctctc aactgctcct caatatgtca
    18841 attttggaaa acataaaata tccagtagac cattaagtga attcggacag ttttcaggtg
    18901 ggttgaaaat tatagatgaa cgggcttttg gaagttggaa attacaattg aaacaagaga
    18961 aagttcttac aaatggagat tttgagcttc cagaagctct ttcctttgtt acctctgaaa
    19021 atacaacggt tatagggaca tccgctgtta cgattttcga aagtgatcaa aagggtgaaa
    19081 gcgatttgtc ctctctttta gattctacag ctcatcgtgg aatcagaata gatattcctg
    19141 tagaaaacca aagaatcgga acctttgagg ggaaactatc ttggatactt gcagatgtcc
    19201 caggaaatta acaggaagag aagaatataa caaaattatt gagtttcaag aagggcacag
    19261 gtaccatagg ctcttgtgtc ccacagagac aagattttgt tatagtcttc tcttacatat
    19321 gattaaagga gcgattttct tgagaccaaa acaacaatta tttataacac tcctagttat
    19381 tttaggggca ctttttaaca atatatcagt gtcagctagt gcatttaatt tttcagtgac
    19441 gccgataact tcagagaatc aaatcgacaa gagaaaaact tacttcgatc ttcaacttga
    19501 tccggatcag gaagtagaag taaaagcgga attgagaaat gatacagaaa aagaagtgaa
    19561 aatcgatatt tctgtgaata gcgcaaccac caattcaaat gtgatggttg aatacggaaa
    19621 aaacgagatt gaaaaagaca tgagcctgat ctttgatctg gtagattatg tgagctatcc
    19681 acagatagtg acgctaaaac ctaaaagtgt ccaaaccgtt gcttttcatg tcaggatgcc
    19741 gaatgagcga tttgatggtg tattggctgg tgggatcaca ttccaagaaa tccagacgga
    19801 aaaggaccaa acagaaacta aggatcaaag cttatcgatt gagaatgaat acacctatat
    19861 tattgctttg ttgatgcaac agacactgaa cgaagttgct ccgaacctta ctcttcatga
    19921 agttaaacca gatcaaatca atgcaagaaa tgtcatctta gcaaacgttc aaaatgatca
    19981 aaaaacctat atcaatcaag tagttattga aacaaaaatt acaaaaaaag gacattctga
    20041 agttttatat caagaggaaa aagaaggatt gcagattgcg ccgaatacaa atttttcttt
    20101 tccaactgct ttaaatggtc aaccgctaac cccaggtgaa tatcacctta caatgactgt
    20161 tttaggaaat gaaaatgaaa atggaaaatt ttcgagaaaa aaaggaaata ctacgatcaa
    20221 ctataccaat caatgggtat ttgaaaaaga ttttacaata gacggaaaag tagccaagga
    20281 attgaacacg acggatgtta cgattaaaaa agacaataat tggatttata tcttagtagc
    20341 ttcacttttc ttattgttta ttttattatg catacttttg tttgttttga gaaaaaaaga
    20401 agagaaagaa aagtaaggtt aagataacaa atagtaaatt tttattattg taggatggtt
    20461 gggctctttt tataacaata taaaatatac cattttatta ataaaaatgc atactagtaa
    20521 atgatataat tctaataaca ggtttgatcg cgagaaaggc caaataaaac gctctccaaa
    20581 atgttaaaaa aaagacagga cgatgtatat tatacaaaca agaatcgctg acaacagtga
    20641 ctcggatcca ggaaatcagt tttatcccca gctagttggt gaatgcatgg aaagtaatat
    20701 acgtagaaag agctgatgac taatgaatta tgagtttttt ttaagcaaag aagatcacag
    20761 aagttattca ttacttaaat atttagaagc aagctcaaat ttatcagagt caatatcgaa
    20821 tgttcaagaa gagttgtcgc tatcgacatt tctcttgaaa aaaacgatcg ataagctgaa
    20881 acatgattta caaaagttcg atctagagaa aaatttgcga gtaaccgtca gtgaattgga
    20941 tatatgtctg gaaattaatg gaaaatgttc tagcaaagct ctgttatctc gttatataac
    21001 ggattcttta tctatgaaaa tggtattggc tttctttcag gaaaaatata tctcagtaga
    21061 aaattttgct aaacagcatc acgtgagtta ttctgtggct tataaagtat tacaaagact
    21121 aaaaagaaat ctaaaaaaat atcaaatttt ttttgaaaag agaaagttg
//
LOCUS       DAMEET010000050        20597 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.051, whole genome shotgun sequence.
ACCESSION   DAMEET010000050 DAMEET010000000
VERSION     DAMEET010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.051"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            295..1404
                     /locus_tag="PB780_001631"
     CDS             295..1404
                     /locus_tag="PB780_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDE0733687.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            1487..2383
                     /locus_tag="PB780_001632"
                     /pseudo
     CDS             1487..2383
                     /locus_tag="PB780_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            2399..3223
                     /locus_tag="PB780_001633"
     CDS             2399..3223
                     /locus_tag="PB780_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDE0733688.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            3255..4565
                     /locus_tag="PB780_001634"
     CDS             3255..4565
                     /locus_tag="PB780_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HDE0733689.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            4713..5972
                     /locus_tag="PB780_001635"
     CDS             4713..5972
                     /locus_tag="PB780_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDE0733690.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            5982..6854
                     /locus_tag="PB780_001636"
     CDS             5982..6854
                     /locus_tag="PB780_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDE0733691.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            6866..7696
                     /locus_tag="PB780_001637"
     CDS             6866..7696
                     /locus_tag="PB780_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDE0733692.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            7736..9919
                     /locus_tag="PB780_001638"
     CDS             7736..9919
                     /locus_tag="PB780_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDE0733693.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            9980..11200
                     /locus_tag="PB780_001639"
     CDS             9980..11200
                     /locus_tag="PB780_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HDE0733694.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            11197..12657
                     /gene="gtfA"
                     /locus_tag="PB780_001640"
     CDS             11197..12657
                     /gene="gtfA"
                     /locus_tag="PB780_001640"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HDE0733695.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            12734..13840
                     /locus_tag="PB780_001641"
                     /pseudo
     CDS             12734..13840
                     /locus_tag="PB780_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            14162..14371
                     /locus_tag="PB780_001642"
     CDS             14162..14371
                     /locus_tag="PB780_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDE0733696.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            14364..14513
                     /locus_tag="PB780_001643"
     CDS             14364..14513
                     /locus_tag="PB780_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDE0733697.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            14667..15332
                     /locus_tag="PB780_001644"
     CDS             14667..15332
                     /locus_tag="PB780_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDE0733698.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(15616..16701)
                     /gene="xerS"
                     /locus_tag="PB780_001645"
     CDS             complement(15616..16701)
                     /gene="xerS"
                     /locus_tag="PB780_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HDE0733699.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            17035..17268
                     /locus_tag="PB780_001646"
     CDS             17035..17268
                     /locus_tag="PB780_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HDE0733700.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            17265..17672
                     /locus_tag="PB780_001647"
     CDS             17265..17672
                     /locus_tag="PB780_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDE0733701.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(18809..19327)
                     /locus_tag="PB780_001648"
     CDS             complement(18809..19327)
                     /locus_tag="PB780_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HDE0733702.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            20445..>20597
                     /locus_tag="PB780_001649"
     CDS             20445..>20597
                     /locus_tag="PB780_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDE0733703.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTS"
BASE COUNT         6994 a         3177 c         3834 g         6592 t
ORIGIN      
        1 aaaaagtaaa catccaaaaa agggtgttta cttttttgtt tactaatatt tttagtaaaa
       61 aatctcgaca aaacattaag taaacagtta tggtgttgtt aacaaaaaga aaacgcattc
      121 aaaacaggag gaattaagat gaaacatttc aaaaaaatag ctttaacagg gatagctgtt
      181 ttaactggga cattactcgc agcatgtggt gggggagata gtaattctaa agacgggcaa
      241 gtaactttaa gctatgcgag ttgagatact gaccaagctc caggactaag aaaaatgtta
      301 gatgaatttg aaaaacaaaa tcctgatatc aaagtagaaa tggagacaac gccttgggat
      361 caatattggg ttaaattaga agctgcaaca acgggaggta atatgccaga tgtagtaacc
      421 atgcactcta gcgaatccta taaatatatg tcacagaatg ctttgatggg acttgatgat
      481 gttatcaaag aaaatgatat tgacatggga aactttactg aaggaattgc tgatttctat
      541 acgcttgaag acgaactata cgcaatccct aaagatgctt ctgtagtggg gctatggtac
      601 aacaaagaat tatttgataa tgcaggtgtc gattatccag atgaaacatg gacatgggaa
      661 acactcagac aagctgcaat tgatttaaca gatgaatcaa aaggcatcta tggttttgca
      721 tcacatattg gaacagagac tggctactgg ccatttattt atcaaaatgg gggagaagta
      781 tttgccgatg acaatacaaa atcaggtttg aataccccag aagcacaaga agctttagct
      841 ttttatcgag atattgcact tgttgatcat gcttcaccaa caattacaga attacaagat
      901 cctgataagt taagtcgttt tcaatcaggt agattagcaa tgatgattga aggaaactgg
      961 tttgcaccgg gatttttgaa aaatgactat ctagttgaaa atgttgcttc tgccgtgtta
     1021 cctaagggaa aagttaatgc aacaattaca aatggacttg gttgggctgc cagtgcgagt
     1081 acaaaatacc cagaagaagt gaaaaaacta atggcatttt tagctagtga ggacgccaat
     1141 aaaatccaag cggaaacggg ggcatcaatc ccagcactaa aaggctttga agacgagtgg
     1201 gctgcttctt cagatacaat tgatttgagt gcttttggaa aaatgttgga gtatggagtc
     1261 cctcgtccat acaataaaga tggattacga gctgaacaag cagaagttca aattattaac
     1321 cgtattctgt ctggtgaagt aactgttgaa gatggaaccg ctcaaattga aaaagaagtc
     1381 aatgcgatat tgagcggaaa ttaaattagt taattataca atatacaggc gaaaggattc
     1441 tatttcgtct gtatattaat ggatgaatac caaaaagggg gatattatga aaaatcgaat
     1501 ttttggaaaa tatcgtaaag atcaagtgat atggggctat atttttattt ttccaacggc
     1561 attaggatta attttattaa atatatggcc agcaattcaa acagcaattt taagttttca
     1621 aaaaacggta gggttcggtg atactgaatg ggtcggatta agaaattacc aaaaaatgtt
     1681 tgcagacggt gaagtttttc agtctttatt aaacacatta atttatgcag tggtttcgat
     1741 acctagtatt gttgtattat ccttgttggt ggcagtttta atgaatagaa aagttaaagg
     1801 attgtcgatt tatcggacaa tttattttct accagtcgtt gctgcaccat ccgcagtcgc
     1861 aatgatttgg cgctggatgt tcaataatga ttatggactg attaataaca tgttggctaa
     1921 aataggtctc gatggaccag catggttaac ggatccatcg attgcgattt attctattat
     1981 cattgtagga gtttggagtg ctattggtta taacatggtg ttattactag ccggcttata
     2041 agaaattcct aaagattatt atgaagcagc ggaaattgat ggtgctagtc caatccagca
     2101 gtttttcaac attacgttac cattagttac accaacatta ttttttgtag ttgtaacaac
     2161 agttatcaat gctttccaag tatttgatgt tatttttatg atgattactc ctaatagtac
     2221 cgccatgttt aaatcacaat cattagccta tttattctat aaacattcat ttattttaaa
     2281 tgacaaggga tatggttcgg caatcgttat gttcctttta gtgattatat taattattac
     2341 tgcaattcaa atgaagttgc agaaaaagtg ggtaaactat taaggaggct atcaaattat
     2401 gaaaaataaa tttttttcaa atgcgccaat ccatatcatg cttattttag gtggtattgc
     2461 aatggcatta ccttttattt ggatgatttt aacttcaggg aaaacgttgg ttgaatctac
     2521 ccaaattcca cctacaattt tccctgagaa atttcagcta agtaactatc aacaagtact
     2581 tgatttatta ccattcatgg ctttttatat caatacattt ttaatggttg ttggacgagt
     2641 gattggctcg gttttatttt cagcaatggc tgcatatgct tgtgcgcgtc taagttttcc
     2701 agggaaaaat atccttttca gcttagtact tattcaaatg atgattcccg gttccatttt
     2761 tattactcca cagtatttat tagcacaaaa attgggtatt ttaaatacaa ttccagcatt
     2821 aatattacca ggggtagtta gtgcgtttgg tacgttttta ttaagacaat ttttcttagg
     2881 tttacctgat gaattggagg aagcggccta tttagaaggt gccaatattt ggacgatctt
     2941 tttcaaaatt atgttgccgc ttgctaaatc aggattggtt gcacttggaa tattcacagc
     3001 gttgtttgca tttaaagatt taatgtggcc attgattgtc aatacttcaa ttgataagat
     3061 gcctttagct ccgggattag caaacttaca aggacaatat gccacgaact tcccacaatt
     3121 aatggcaggt tcgttattag caatttggcc aatagttgtg atattcatcg ttttccaaag
     3181 aaaattcatt gaaggaattg ctacaacagg tagtaaatta taaaactatt aattagtgat
     3241 tggagagtaa gtacatgaca aaaattacat ttattggtgc aggaagtact atttttgcaa
     3301 aaaacgtatt aggcgatgcg atgttaactc caagtctaca agatgcacat attgcattgt
     3361 acgatattga tgaaaaacga ctaaaagaat caaatttaat gcttaaaaat attaataaaa
     3421 attcgaatga aaatcgtgct gatattgttt cttattatga tcgcaaagaa gcgttaagag
     3481 aggctgattt tgttatcaat gctattcaag tgggtggtta taaaccttca acagtgattg
     3541 attttgatat tcctaaaaaa tatgggttgc gacaaactat tggtgacacc atggggattg
     3601 gtgggatttt tagaacccta cggacactac cagtaatgtt agactttgct aaagaaatgg
     3661 aagaagtttg tccaaatgct tggttattga actatacaaa tccgatggcg atgttaacca
     3721 tgggtgtttt aaaagcgacg agtattaaga ctgttggttt atgtcatagc gttcaagtat
     3781 gcgtaccaga attgtttgag catttaggaa tagccgatca atatgactta aatgactttc
     3841 aatggaaaat tgcaggcatt aatcatatgg cattcttgtt ggaaattaca aaaaatgggg
     3901 aagatttcta tccaacaatt cgtcagttag ctgcagaaaa accaaatcca catccagatt
     3961 ctgtacgttt tgaattgttg aaacgttttg gttactatat tacagaatca agtgaacaca
     4021 atgcagaata ccatccatac tttattaaat caaaataccc agaattgatt gacgaattag
     4081 ggattccgtt ggatgaatac ttacgtcgtt gtgaacaaca aattaatgat tgggaaagca
     4141 tgcgagagga cattgtaaat aatggggagt taacacatac gagaagccgt gaatatgctt
     4201 catatatcat ggattctatt acgacaggaa ctccgtcagt gattgcagga aatgtcttaa
     4261 ataaaggctt aatcacaaac ttaccgacag atgtttgtgt ggaagtacct tgtttagtcg
     4321 ataaaaatgg cgtgcaacca acctatgtcg gtgaattgcc aacacaatta gctgctttaa
     4381 atcggacgaa tatcaatgtt caagaactga cagtagaagc cgctttaaca ctagataaat
     4441 caaaaattta tcaagcagcg tatttagatc cacatctatc atctgaattg tctttaacag
     4501 atatcaaaaa tttagtggat gatttaatcg aagcacatgg ggactattta cctgaatata
     4561 attaaaaaga agctgaaaga gtaaggaatt ttcaattcct tactcttttc ttagttaaat
     4621 ttagtaaaaa tattgacaaa acacttagta aacagttaca ttagtgttaa caaaaagaaa
     4681 acgcattcaa attgtataag gaggagcata aaatgaagtg gtctaaaaaa attattttag
     4741 cttgtatagc actaggagca acgatctctt tgactgcttg tggaaaaaat caggaagatg
     4801 caagcggtaa ggtagaaata gagtttttta atcaaaaaaa agaaatgact caaacgattc
     4861 aggagattgc taaagatttt gaagcaaaaa accctgatat tcatgtaaaa gttgtggatg
     4921 taccgaatgc tggtgaagta attaaaacac gtatgcttgc aggcgatgta ccagatgtca
     4981 ttaatcttta tcctcaaagt atagaattga aagaatgggc gaaagctggt tatttagagg
     5041 acttaaccga agaaccgtat ttagagaata tcaaaaatgg atatgcacaa cgttttgcta
     5101 ttgaggatcg tgtatatagc ataccattaa ccgctaatgt ctatggtttt tactataaca
     5161 aaactgcttt tgaggaaatg gggattcaag caccagaaac ttgggcagaa tttgaaaaaa
     5221 tagtagctga gattaaagat caaaacaaag taccgtttgc gattgcagga gcagaaggct
     5281 ggactttaaa tggttatcac caactagcta tagcgactgc tgctggtggt gaagaggaag
     5341 cgaacaatgt ttggcgtttc tcagaagtta atggaattaa tgctgaaagc aaagaaatgc
     5401 aaaaagattt taatcgatta gatttgttaa gagaaccaaa agctttgcaa aacaactggc
     5461 aaggtgccgg ttataacgat acagtagtta cttttacaaa aggtgatgcg ttgattatgc
     5521 cgaatggttc atgggcaatg ccgatgattc attcacaaaa tccagatttt gaagtaggaa
     5581 cttttccttt tccagcagat gaagcaggtc aaagtctaac gattggcgca ggagacctag
     5641 ccttatcagt ttctgcaact tctcaacata aagaagaagc caaacgtttt gtagaatata
     5701 tgacaacacc tgaagcgatg cagaaatatt atgatgtaga tggtgcaccg tgtgctgttg
     5761 agggcgttat cgagaatact caggattcac cattaagtgg tttaactgaa cttgctttta
     5821 ctgatcgtca tttagtttgg ttagccaaag attggaatag tgaaaatgat ttttatacct
     5881 taacaacaaa ttatttacac aatggcaatc aaaaagccat gattaacgca ttaaatgctt
     5941 tctttaatcc aatgaaagct gacggaaggt aggaaaataa aatgaaaata aaagaaattt
     6001 taaatcgtta ctgggcaatc atttttgtag caattccaat tattttgcag atgatttttt
     6061 tctactttcc aatggttcaa ggtgcttttt acagtttaac taattggact ggtctgactt
     6121 ataattttga ttttgtgggt ttgaacaatt ataagattct tttttctgat ccgaagttta
     6181 tcaaatcgat tggcttcact ttaattgtga cgctttgcat gattgctgga caaattggtt
     6241 ttggtatttt gattgcgcgg gcgttgaacg caaatattaa agggaaaaca ttctttagag
     6301 catggttctt ttttccagcg gtcttatcgg gcgtggttgt ttctttagta tttaaacaaa
     6361 tttttaacta tggtttacca aaaattggta gtatgttagg cattgaattt ttacaaacca
     6421 gtcttttagg aacaacatgg ggagcgattt ttgcagtaat ttttgtttta ctttggcaag
     6481 gaattgccat gccagtcatc attttcttag cgggttttca aagtattcca caagaaatta
     6541 ttgaagctgc tagaatggat ggggcgaatg ataagcaaat tttttggaat attgaaatca
     6601 cctacttatt accaagtatt tcaatggtat ttattatggc attaaaaggt ggcttaacag
     6661 catttgatca aatttttgct ttaactggtg gtggaccaag cgatgccaca acatctttgg
     6721 ggttacttgt ttataattat gcttttaaaa acaatcagtt tggttatgcg aatgctattg
     6781 ctttagtctt atttatctta attgggattg tttcactggc acaattaaaa ttatcgaaaa
     6841 aatttgaaat ctaggaggtt tcccaatgaa aacatcaaaa gaaaaaaata tttctaaata
     6901 cttgctttta ttattagggg gcttcatgat tttagttccc ttagtttcaa cgctattcgg
     6961 ttcatttaaa acaacacccg atattatgaa taatttcttt cgttttccta atccagcgac
     7021 tttggaaaat ttccgaagat tgttagcgga tggaattggt cattactttt ggaattcggc
     7081 aatcatcaca atcattgcag tagttgctgt gacagcaatt attcctatgg ctgcttattc
     7141 actagcaaga aaaatgactc gtgggaaagc atacggattg atgtatttat ttttaatttt
     7201 aggaattttt gttcctttcc aagtaattat gattccgatt accgttatga tgagtaaaat
     7261 tggtttagca aatatgtggg gattaatctt actctatctc acttatgccg taccacagac
     7321 actattttta tatgttggtt ttattaaact aaatgtgcct atcagtctgg atgaggctgc
     7381 tgaaattgat ggggctagtc actttacaac ttatttcaaa attgttttcc caatgttaaa
     7441 accaatgcat gcgacgacta ttattcttaa tgctctatgg ttttggaatg atttcatgct
     7501 accattatta attttaaatc gtgattctaa tatgtggaca ctaccattgt ttcaatataa
     7561 ttatacggga caatatttta acgactatgg tccaagtttt gcttcctata cagtaggaat
     7621 tgtcacgatt acactagttt atcttgcttt ccaaagaagt attatagctg gaatgagcaa
     7681 tggagcagtg aaataaccaa taaattattc tcattataaa ggagttcata aacttatgat
     7741 tatatttgac aatcacacac aaacatttca tttacaaaat aaaaaaatta gctatctatt
     7801 aggaatcgaa gaaaataatt atttaacgca tctttatttt ggaaaaaaga ttacgcacta
     7861 ttctggtggg tatcgctacc cccgaacaga tcgttccttc tcacccaaca atgcaaatgc
     7921 gcaagatcgt ttattatctt tagacacatt accattagaa tttccaggat atggacatgg
     7981 tgattttaga gaaccggcgc ataatattaa attaggaaac ggtagtcgtg taaatgattt
     8041 tcgttatgat agctacgaaa ttcaaaaagg aaagcccagc ttagcgggat tgccagcctt
     8101 atatacggaa gatgatgatg aagctgaaac gctgattatt caattgaaag acgcgattag
     8161 tggattgact ttacagttgt tctacacaat ttatcaaaat cacagtgtaa ttattcgttc
     8221 tacgcaatta gtaaataata gtgatgaaac agtggagatt aaccgcttag ctagtcaatc
     8281 aattgatttt ccaaatcgtc cactcgagtt aattcattta aatggtacct gggcaagaga
     8341 acgtcaaatg actagagaaa aaattcatac agggataaaa gtattagata gtaaacgcgg
     8401 atcaagcagc catcaacaaa atccgtttgt tgctttagta gatgccaaaa cagatgaatt
     8461 tcaaggagag gcatatggat ttaatttagt ttatagcggt aatcacgaaa cggtgattca
     8521 aaaagatcca tttgaccaaa cacgggtcat tatgggaatt aattcattta attttggttg
     8581 gcaactaaaa actggcgact catttcaaac gccagaagtc gtgatggttt atagtaatga
     8641 aggtctgaat acgttatcac agacgtatca tgaattgtat aatcaccgtt taattcgtgg
     8701 aaactatcgt ttaaaagaac gtcccacgtt aatcaacaac tgggaagcaa cgtattttga
     8761 ttttaacgaa gaaaaaatca tgaatattgt cgatgatgcg aagaacttgg gcattgaaat
     8821 gtttgtttta gatgatggtt ggtttggcgg tcgtgaaaat gacttcactt cattaggtga
     8881 ttgggttgaa acagaaggca aacttgcttg tggtttggaa aatttagcca agcaagtgca
     8941 tgataacggg atgaaatttg ggatttggtt tgaaccggaa atgatttctg aagatagtga
     9001 actatttcga gcacatccag attgggcatt gggtattcca ggtcgtgacc gctcaattag
     9061 tcgaaatcaa tatgttcttg atttttcaag acaagatgtt cgtgataata tctatcaaca
     9121 aatgacggcg atttttgatc gtgtaccgat tgattatgtg aagtgggata tgaatcgaaa
     9181 tatgacagaa gtctattctg tcttgttgga ctcaacgatg caaggtgaag tggctcatcg
     9241 ttatatgtta gggttatatg aatttttaga aaagttaaca acgaattatc caactatttt
     9301 atttgaaagt tgttcgggtg gaggcggtcg ttttgatgca ggtttcttgt attacatgcc
     9361 acaaacttgg acgagcgaca ataccgatgc cgtggctcgt ctgaaaattc aatatggaac
     9421 cagcttagtg tatccaattt caagtatggg tgcgcatgtt tcagcagtcc caaatcatca
     9481 aacaggaaga gaaacgagtt taactattcg tggcaatgct gcgatggctg gtgtttttgg
     9541 ttatgagcta gatctaacgc aattaacttc tgaagaaaaa gcagaaatta aagaacaagt
     9601 tgccttttat aaaaagcatc gccaactttt gcaatatgga actttttatc gcttggaaag
     9661 tccgtttgaa ggcaatgata ctgcatggat gtttgtctca aaagaccaaa cagaagccat
     9721 ggtcttttat ttccgagtat tagcagaagc tgcgcatcca ttagtcacat tgaaattagc
     9781 aggattagca gaagaagcaa cttatcaaat ggaagatgta acatttgctg gcgatgaatt
     9841 gatgaatctc ggtttttata ttgactcaga actacatggt gattatgcga cacaacgctt
     9901 ttatttgaaa cagatatagt agcttgaatt tacaatataa gtgcaacacc aaaagagact
     9961 cattccaaac gaagaaaata tgttttattt acacgcattt actaaaaagc agcctgattt
    10021 gaattgggaa aatgaagaag tacgtgaaga aatctatgca atgattaact attggttaga
    10081 taaaggacta ggcggattta gaatcgatgc gatattaaac atcaaaaaaa aactagagta
    10141 tggcatcttt gaatctgatg gagaagatgg gttagccttc attgggccct ggattcttaa
    10201 tcagtcgggc attgaagtgt ggttaaaaga aatgagagag cggacattta aaccacataa
    10261 tagtatgacg gtagcagaag ccgatgttcc taatgaacgt ttagatgaat atattggtga
    10321 agatggctat tattcgatgg tttttgattt tagttatacg gatattgatg tacctgaaac
    10381 tggtgaatgg tttaaagata gccaatggac atggacggat atgcgtacca atatttttac
    10441 gaatcaatta gttacgcaag ataaaggttg gggcgcctta tatttagaaa accacgatca
    10501 accacgttca attaataaat atattccaga agaatggatc aatgattata gtaaaaaaat
    10561 gttggcgacg ctttttatgt tactaagagg tacccctttt atttatcaag gacaagaact
    10621 aggaatgacc aacatagaaa tggcatcatt agaagatttt gatgatgtag ctacgcattc
    10681 tcagtataaa cgggcattag aatatggatt ggcaccagag caagcattga aagctgtcaa
    10741 taaacgaagt cgggataatt ctcgcacacc tatgcaatgg aatacgcaaa aaaatgccag
    10801 attctcaact tctgataatg tatggttaaa agtaaatcct aattatccag aactcaatgc
    10861 agaagctcag ttaaccaatc aacagtcggt attcaattat tatcatcaat taattaaatt
    10921 aagaaaaaat agtgtataca aagacgtgct aatctacgga caatttctac cagtgaaaga
    10981 taaaaatgaa catattttgc tttatgaacg ccaattagaa gataaaatcg tattagttat
    11041 cttaaacgtt acagcaacag aacaagaata ttatgtagat gaacagtacc aacaagtatt
    11101 aatcaataat tatgaagatg ttttattaga tcaaagtcga aaattgcgtt tacgaccgtt
    11161 tgaaagtatt gtattagcta attatggagg acaattatga aaattaaaaa cgaagcaatg
    11221 ctcatcacct atgcagatag tttaggaaaa aacatgggcg aattaaaaca agtcttagac
    11281 aaacatttac aaggtgttat tggtggtgta catctgttgc ctttttttcc atcgacaggt
    11341 gatcgaggct ttgcgccgag tgattatacg acagttgatc cagctcttgg aacatgggaa
    11401 gatgtggaag cattaggtga agattattat ttgatgtttg attttatgat taatcacatt
    11461 tctcgtgaat caaaattttt ccaagatttc aaaaaaaacc atgagcagtc accatacaaa
    11521 gaaatgttta tccgtattca tgaatttttc ccagaaaatc gaccaacaca agaagatatt
    11581 gatttaatct acaagcgtaa agacaaagcg ccgtttcaaa cggtagaatt tgcggatggt
    11641 acgacagaag aggtttggaa tacctttggg gaagaacaaa ttgatttaga tgtgaccaaa
    11701 gaagttgtga aagaatttat tcgagaaacc atcaaagata tggcgaacca tggctgttct
    11761 ttaattcgat tagatgcgtt tgcttatgcg attaaaaaat tagataccaa tgatttcttc
    11821 gtggaaccag atatttggga acttttagac gaagtgcgtc aagaagcagc gaaatatgac
    11881 gtcgaattat taccagaaat ccatgagcat tattccattc aaatgaaaat tgccaatcat
    11941 gattattata tctatgactt cgccttgcca atggtcactc tgtattcact ttatagtgga
    12001 aaatcagaac gtttagccaa ttggttgaaa atgagtccga tgaagcagtt tacaacattg
    12061 gatacgcatg atggaattgg tgttgtggac gctcgtgatt tattaacaga tgaagaattg
    12121 gattacacct ctgaggaatt gtataaagtc ggggcgaatg tgaagaaggt ctattcaagt
    12181 gccaattaca ataatttaga tatttatcaa attaacagca cctattatag tgcattaggc
    12241 gataatgacc gtagctatct gttagcacgt gcgattcaag tatttgcgcc agggattcca
    12301 caaatttatt acgttgggtt attagcagga aaaaatgata ttgaactgtt ggaagcaacg
    12361 aaagaagggc gtaatattaa tcgtcattac tatgatttag ccgaaattga agaagaagtg
    12421 caacggccag tgattcaaca attatttgca cttttaaaat ttagaaatga atctgcggcg
    12481 tttgatttgg atggaacgat tgaagtagca acgccatcag aatcagaaat agtgattaca
    12541 cgtaaaaata aaaacggtag tgatagtgca acattaacgg ctaacttgga tacgaaagaa
    12601 tttatcattg agtctaaaga tataaaaatt ttcactagtg ttgcattaaa attttaacct
    12661 attccataga acaaaaaatc ctttatacta gcacttggat gacttatcca agaccaataa
    12721 aaaggactca tacatggata agtataaaac aaatacagca tttaataaat ggttttctgc
    12781 cataaaatta ggaaaacttt ctgtcaactc ccaaaaatgt atttttgatt acaattcgta
    12841 tcgtaaaaaa ctgtcttttg aagctgtgct caagctcttt ctctacgcag ttaatgaaga
    12901 aaaagaaagt ttgcgtgatt tgagtacagc tcttatcgac cgatcgctcc aactagaaac
    12961 cgatgtgtca tcgattagtc atacccaatt gtctcgagca tttaatgcac ttgaacctaa
    13021 agtgttagaa gaaatttttc aacaactgtt agaaaaagtt caacatcaaa tcaaaccaac
    13081 caagcgaaat catctgtatc tgattgattc cagtacgttt tcgcttagtt taaaacgtca
    13141 taagtgggcg actttccgtc aaacaaaatc aggtgtcaag ttacacttaa actattgtta
    13201 tatggacgat actacgatgt atccaactga ttttacctta acaaatgccc atgaacatga
    13261 tctcaatcaa ctaccagtat tggtgaatca accagaggca acttacgtgt ttgatcgtgg
    13321 gtattaggac tttgaaaaga tggatcagat gcattgggat ggctatttct tcgtcactcg
    13381 gattaaaaaa aacaccaaag ttcacgtgat agatactctg gaaacatcgc cagaaacaga
    13441 aattctacga gacgagctgg ttcgtttagg atctaaaacc tatctaaccg ccaatttccg
    13501 actagtcact gttcaagata agaacggacg tgtgtttcaa tttatcacca atcgaatgga
    13561 cgtctcttca aaagaaatct ctgatatgta tcacgctcgt tggcaaatcg aattgttttt
    13621 caagcacatc aaacaacaca tgaccattaa aaccttcttt tctcaaagtg aaaaaggcgt
    13681 gcagaatcaa ttgattttaa cgatgatttc cgctctcctc acgtttctca tcaaattgga
    13741 gacgaaaacg gaaaaatcag tgttccaaat caaacgattc tttcgttatt tactgtttca
    13801 gccctttgaa tgttgtctag aaaaattaat tcccacatga gaaaaaagaa atttggctcg
    13861 ttacttttgg ttttcagttg aataacagat aaaaaatttt ctggaaaaag tcatcctctg
    13921 cgtcagcaca gttttttttg acttattttc agaggaaata gttttcagaa tattctaaaa
    13981 tgttttatgc aacactagtg atatttttta gaattagatt tctaatgtgc attttttgag
    14041 tattaaggag caagtataca aagatttata aagctgatta aatttgaata aaaatatgca
    14101 ggaacgctac tatttttagt agtgttcttt tttatatttg aataaaaaat gtggtggtta
    14161 aatggtggtt ataacaaaaa atgaattaat gaaattaggt tatgggaagt atcagtctga
    14221 gagaatcata cgacaggcaa aagcaattat ggtttcaaaa agctatacct attacgaaaa
    14281 tagaagatta ggactagtac ctgttgaatc tgtagaagaa gttttaggta tgaagataga
    14341 ggtagaggag gattttcaga atcatggtta attttaaaaa agataaaaat gggaaatatc
    14401 attttactgc tagtttggga ttcgacaaac gaacagggaa aagagtacaa aaaaacgtag
    14461 aggggttaaa acagttaaag aagaaaaaaa gcgtataccg aacttgtttc taattatgga
    14521 aaagaagcag aaacttctca aagtactatg ttgtttgagg agttttttta taaattattc
    14581 ctaccttatt atcaagggaa agtagaagaa agaacatata aaaatcggtt atctacaatc
    14641 aaaaaaactt ttcttatttc tttaaaatgg agttaagaga aatacgtcca attgacattc
    14701 aaaagtggca aaataattta ttgagcaaat ataaaaattc ttatgtaaga aacatgtatg
    14761 ggatttttca gatgtcatta gatcgagcag ttgttttggg actacttgaa aaaaatcctg
    14821 caaaagttat tggaaatgtg aagaaaagga aaactacagt tgatttttgg acaaaagaag
    14881 aatttacaaa agttatttca actttttata ttgatgattt gtaccaacat tttggtttta
    14941 tttgtatgtg gttacttttt atgaccgtta tgcgtttggg agaagccact gcattaactt
    15001 ggaaagatat tgattttgaa aataaaaact tgcgtgtgaa taaaacactc tattataaga
    15061 gagccaaaac ctatgagttt gtagaacccg aaacggcttc tagtattcgt acaatcgaac
    15121 tagacgacaa tacaattacc tttttaaaag cttggaagga gcgtcaagtt tcaataggag
    15181 gcatgtcgtt tgtactttct tataatggaa cacctactca gaaatataca atccgtcata
    15241 ttatgagaag acatgcaacg atcgttggtg ttcatataat ccgcatacac ggattaaggc
    15301 atagtcatgc ttctttgctt atatcacaat aaaaagatgc acttcgaatt aaagaaaggt
    15361 taaaacatgc tgatgtacaa acgactttag gaacttatgg tcatttgtat ctaaattcat
    15421 tgagagagat aacaaatgat cccacagatt atatccaaac taatttttct aaaatcaaac
    15481 cgacacctat ttacgataat aaaaaataaa tgataaacaa aagtaggatc acaccaaaag
    15541 aaacacacta gaaaaactat aatacaggct tttctagcgt gttgtttctt atttccgcct
    15601 atttatacat tatactcata aagcatttaa tgcatcgatc gtagccgcat tgtctatatg
    15661 ggtatataaa gttgttgtat tggtacttga atgtcccaat tgttgggcag ttaataccaa
    15721 tgaatttgtc tgttggtaga gtctagtagc taatgtatga cgcagtttgt gagggctgac
    15781 ttgaattcta aagccttttg aatattttgc taccatccgt tcgattgcaa taccggatat
    15841 acgcttggct tctccttggt aaattgctag aaataaagct ttttcgtttt ctgttacttt
    15901 atatcgttct ttccgaatag ctagataatt agctaaatat tccattgcga atgctgctat
    15961 attgacagca tctcttttat ttccttttct agttactacc acattattct tatctaaaga
    16021 taggtcatct aaattaatat ttataacctc ggagagtctc aatccagatc ctaaaatcag
    16081 tgcattgatc gccaggtctc tttctacatc tcgttgataa tagatcaaag ctcgtttact
    16141 caaagtattt ttatattcat tggcaatata atctagataa ttttgtgtat ccttatctag
    16201 aaataatttt ttctcaatgg cattggcacg tgcagctaac gtagttttat ctgtgatggt
    16261 tgctacttga tccataggat tattgtctaa gtatggtttt cctagaggat tattggaact
    16321 tttcgaaaga tatttaaata gaacctttaa tgccgtgata tttctattga tagtagaagt
    16381 agatagacct aagttattat tatctttgag cttagttttt gttgtctctt tttttacatt
    16441 ttcttttgtg cgacgtttca aatgagattt gtacaagttt aggtcatagg cagttaactg
    16501 tcttatcgag gtgatagaag tatcaggaac gatagactca ctcagaagcc attcataaaa
    16561 gagcttataa ttttttagat attctaaagt ggttcgaggt gattgatctt tatcctcacg
    16621 atcttgaaca aaatctttta tataatcggg catatgaagt atgattgtat ctattttttg
    16681 atatatttct gtatttttca tgtcattcat cctttcaata agtaggggag aagagtatct
    16741 ctataattgg actataccaa ttatagaatg ttttgtcaat tggtatgctc taaaatttat
    16801 taatttgttt caaaaaaagc atataaacag gtatacgttt aataatttaa aataaatggt
    16861 atacataaat agggaaggaa atagaaggga gcaatctaaa ttttttgaat tttcttaatt
    16921 aattatatag aaagaacaat ctttctatag agttagacat gagagggact atgcttgatt
    16981 taaaatcaac aagcgtatat actgtatata caaagaaaaa ggagggagaa gactatggca
    17041 attaaagaaa gaaaatttag ggaagtgggg aactcagtag tattaactat ttcaaaggag
    17101 tttcttaaag aaaatgggtt aaatcccgga gatacggttt ttttagatga agaaaaactt
    17161 cgagcggcaa ttacaaaaaa acaagcctta tctgatgaaa ttgatttagc aatatctcaa
    17221 gcattaactg aatatgatac tacttttaga gagcttgtcg ataaatgagt attcattacc
    17281 tagaagccaa agatattata aaaatgaatg taatccaaat aaaaaaatac tctcctaatg
    17341 aaccccttgg tgtaaaggat gcgaatgctt tagaaatgtg tgtagggcaa cttcgagcct
    17401 cagcttttgg tgaagaggtt tatccttcga tttatgaaaa agcagctatc ctacttatac
    17461 aattgataaa gaaacatcct tttcacaatg caaataaacg tactgctttc ttagctactt
    17521 ttgtcttttt gaaaatcaat ggacagcttt tagctattga tcaaaaagat gctgtaaatt
    17581 tagcggtata tattgctacg tatgataagg atttcgatca actaaaatat gaagtgatca
    17641 acactataaa gacacatacg cagcccattt aaggagttac tgtatgtgtc tttatagggt
    17701 tattagctgc tatagcgaaa taattgaagt aaattacaaa tagctataat tcaaatccaa
    17761 ctatatagaa aacgtaaatt ttctatatag ttggattaag ataacgacta tttattttat
    17821 catattttta ctatacccta tacttttact tggtatattt atgaataatc gcaaatttac
    17881 ccctatacta ctagtttgta agttagatta ataaaaatac taaagttatt tgttctttta
    17941 ttaaatacac ctacaactta taaattgatg gtatttagat aacacgaaag aataaaaata
    18001 acagctgata gtaaagtttg ttgacgattg ctactcaatc gaaagtgagt tagtgcgtat
    18061 gaacttgctt ctgaaaactt atgaaatgaa agacctttta catcttgcag aaatcgttac
    18121 tgggattaaa ctaagcaata aaaactaatg ttcccctcat tctttgaact tctactattt
    18181 gatttattta ttctgataaa tgacgatttc accttcagga tcttcaaatc ccgtatagaa
    18241 tttatttaag atctcttcag tatcagtaat aggataggcg ataggccaca taccctttga
    18301 tggttagaat aaactgctca aaatctgtat cgtatgttgg ctttagcata ttaacaaagg
    18361 ctatctccaa attataaacc ataagcgttg aatcgttctt aacaatttca attcttccta
    18421 acccaaagtc agaagatata attaccggac ttgccttacc cttcatgtgg gttgttgaga
    18481 ttccatattt aaagggaatt gtcggtacta tcggcggtct gaaaaacatc ccaaaaataa
    18541 aaaaatcttt cccctgctag tctcctaagt ccctttgtag cggatatggc accataatct
    18601 cattttatcg aagtgagtca tggtttatct gttggccaac caaaattgat acaatctaac
    18661 gatgcaccct ctacggcatt ctaggggagt gcacttgcat tcacccaata aaaaccggca
    18721 gttagcaaag aacttatgta gtcgtatgtt cttactgtta acatacaaga ggtctccttt
    18781 ccacaattgc ttagcagtaa acgggaattt acaaagctaa tctcttccat tttcttatct
    18841 ttgttctaaa agtcccaaat ggagcaactg tatttacatg aataaattta tatacttccc
    18901 atacagccgt tttggttgcc tcgtctgccc attttctttt gtgtgcagtg aatagatctt
    18961 catcactcat tgaatcaatc atcatgttaa tatcatcaat attacgattg agaatttcct
    19021 ctaattcttt caatggtaga tgtgcatagg tatttgtaaa ccacttatat aactctccta
    19081 actgattcca tttaaattga tcagaaggcg ttttaacaga taatccattt ctttcatcat
    19141 tttcccattt tagaattaaa ttcgtccaac caacttgata tgctaaattt tcagcaggtg
    19201 ttctatccac ttcctcaact cttttatcct tcaattcttc aggaatagtt tcaaattcta
    19261 aaatatattt agtataagtc ctatttattt ctttttttaa ttcttcttta tttttatatg
    19321 ttctcattat attacctcca taaattccta attgtctcct ttaattcatc tataatttca
    19381 tccacgatat tttaatccgt tctacaatgc atatctgata tgcattgact gtctaactca
    19441 tatgtgaatg atttataaaa tatcccaacg tctcatctaa agaaatgttg atcgtcagca
    19501 aggagctaaa aaatacgaat atatttcttt tgtagtaaaa tcggactggt ttgcatatca
    19561 ttttcttcgt aaatatcaaa atcatgttaa ctactatcaa ttactaataa aaactaccac
    19621 tttaagcgtt aagaactgtt aagtttagtt aagtgaggat agttcacaaa atccattgtt
    19681 aaacttcacg aaagtggtaa aactcaaaat gctttatata ggattacagc atcgctcttt
    19741 cttcgattgc gcgttgggta aaaaatatta tgaagtaaaa attgacgatg acacgttcct
    19801 tacggctcat cagatgaaac agctacaaaa acgaaatgat caactcgaag aaaatttaat
    19861 tctaaaaaag cgattgccat attcacgcct cgctcgacga actattaaag gtcaagattt
    19921 acctgaagat catccttttt aggaaattga agctttttat gttgcacaat gcatttataa
    19981 tactacaatg atgttttcac cagatattct tctagggtgc ggtgtaatga aacagataca
    20041 cttgcaaaag aaaattcacc gcgaatttac taaaattatg aataattatg ttaatcttcc
    20101 gaaatttttt tccaaaatta agggatcaag ctggtgttat agattgttta aggttggcaa
    20161 aaaaataact tctttttaaa caattataga aaaccgatac tctgttaagt tggttgacac
    20221 ggtgtcgatt tttatgactt tcttattctg aaacttcact aacggacagt acctaaaaag
    20281 tctatagtat ggacgtttac gagtactatt ttatcaaaaa tgctgctcat ttcaacttat
    20341 atgttttagt gaactacccc cacttagctt ggataggcta caattaacta gacagaaaaa
    20401 ttaaggtgtg tagactagaa gaaaacatac caggaggaat ttttatgtct aagagaacac
    20461 gaagaacttt ttcacaagaa ttcaagcaac aaatcgtcaa tctttactta gctggaaagc
    20521 cacgtgtaga aatcattcga gaatatgaac taacggcttc agcatttgac aaatgggtaa
    20581 agcaatctaa aacgagt
//
LOCUS       DAMEET010000051        20430 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.052, whole genome shotgun sequence.
ACCESSION   DAMEET010000051 DAMEET010000000
VERSION     DAMEET010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.052"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(25..1803)
                     /gene="uvrC"
                     /locus_tag="PB780_001650"
     CDS             complement(25..1803)
                     /gene="uvrC"
                     /locus_tag="PB780_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDE0733704.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(1985..2299)
                     /gene="trxA"
                     /locus_tag="PB780_001651"
     CDS             complement(1985..2299)
                     /gene="trxA"
                     /locus_tag="PB780_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDE0733705.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(2386..4746)
                     /locus_tag="PB780_001652"
     CDS             complement(2386..4746)
                     /locus_tag="PB780_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDE0733706.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(4861..5409)
                     /locus_tag="PB780_001653"
     CDS             complement(4861..5409)
                     /locus_tag="PB780_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HDE0733707.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            complement(5467..5904)
                     /gene="zapA"
                     /locus_tag="PB780_001654"
     CDS             complement(5467..5904)
                     /gene="zapA"
                     /locus_tag="PB780_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDE0733708.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            6106..7023
                     /locus_tag="PB780_001655"
     CDS             6106..7023
                     /locus_tag="PB780_001655"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HDE0733709.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(7279..8190)
                     /locus_tag="PB780_001656"
     CDS             complement(7279..8190)
                     /locus_tag="PB780_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDE0733710.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(8593..9171)
                     /locus_tag="PB780_001657"
     CDS             complement(8593..9171)
                     /locus_tag="PB780_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDE0733711.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(9191..9859)
                     /locus_tag="PB780_001658"
     CDS             complement(9191..9859)
                     /locus_tag="PB780_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDE0733712.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(9874..10944)
                     /locus_tag="PB780_001659"
     CDS             complement(9874..10944)
                     /locus_tag="PB780_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDE0733713.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(11115..12479)
                     /locus_tag="PB780_001660"
     CDS             complement(11115..12479)
                     /locus_tag="PB780_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDE0733714.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(12476..14035)
                     /locus_tag="PB780_001661"
     CDS             complement(12476..14035)
                     /locus_tag="PB780_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDE0733715.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(14091..16571)
                     /locus_tag="PB780_001662"
     CDS             complement(14091..16571)
                     /locus_tag="PB780_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733716.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(16555..16890)
                     /locus_tag="PB780_001663"
     CDS             complement(16555..16890)
                     /locus_tag="PB780_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733717.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(16904..17938)
                     /locus_tag="PB780_001664"
     CDS             complement(16904..17938)
                     /locus_tag="PB780_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDE0733718.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(18028..18708)
                     /locus_tag="PB780_001665"
     CDS             complement(18028..18708)
                     /locus_tag="PB780_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDE0733719.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(18992..20095)
                     /locus_tag="PB780_001666"
     CDS             complement(18992..20095)
                     /locus_tag="PB780_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDE0733720.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT         5805 a         4289 c         3504 g         6832 t
ORIGIN      
        1 ctttctttgt aaagtgccct ttcctcattc ctgatgtaat ttatcaaata cgttttgtgc
       61 gacatttttc ggcagtccga tttcttgtaa ttcttctgtc gtggctgctg tgatattttt
      121 caacgatttg aactctttaa gcagcatttt ttttcgtttt ggtcctaacc cttcaatttc
      181 atctaatcgg gaagcaaaac tgtttttact gcgtagctgc cgatggaaag tgattgcgaa
      241 tcgatggact tcatcttgga ttcgctggag taagaaaaac tctggtgaat ttcgctcaag
      301 cggtactaca gccaaatcta taccgaatag caactcattt gttttatgtt tatcgttttt
      361 tgccatgcca gcaataggaa tatcgattcc taattgattt tctaagacat cttttgccac
      421 atctacttgt ccttttcctc catcgatcaa gatcaaatca ggtaaaggca acccttcgcg
      481 aatcactcgt gaatatctgc gatagatgac ttctctcatt gatgcataat catccggtcc
      541 gttgactgtt tttattttat atttccgata ttcttttctt gccggttttc catcaatgaa
      601 tacgaccatc gcagccacag gattcgtccc catgatatta gagttatcaa aagactcgat
      661 acgtattggg gtcgggatat tcatcgcgtt tcctaaacgt tcaactgctc cgatcgtccg
      721 ttcctgtttt ctggcaatca gatcaaaccg ttcattcaaa gcaactgagg cattcttatt
      781 cgccaattca actaattttt tcttttctcc tcgttttggt tgcagcactt tcgtagctaa
      841 caaagcttct acactgggct tgtcgatcgt atcaggaatc aagacttctt taggaatgaa
      901 atgctcattt tcctgataaa actgaccgag gtaagtcaag aaatcttctt ctgcttcgtt
      961 atagaaagga aacattgaaa catctcgttc aattagcttt ccttgtctta cgaaaaacac
     1021 ttggacacac atccatcctt tgtcaatcgc ataaccaaat acatctcgat ccagtaaatc
     1081 agtatttgtc attttttggc gagtcatgat tgtctcaatg gcacggatct ggtctcggta
     1141 ttctgcagct ttttcaaact ccatgttttc agcagctttt tccattttct gatggatctc
     1201 tgactcgatt ttttcgtgtc ctccgtttaa aaactttttc acttcatcaa cgattttcgt
     1261 atagactttc ggatctacgt cgaaataata aggacacaga cattgtccca aatgatagta
     1321 aagacaaggc tccttcgtct gactaggttt gcattttctc agaggaaata atcgatcgag
     1381 gatcttcttt gtctcattcg ccgcaccgac atcgggatat ggtccaaaat acagtgcttt
     1441 gtcttttagt actttacgag taatcaccaa acggggatat ttttcattcg ttatcttaag
     1501 gaaaggatag gttttatcat cctttaacat aatgttgtat ttaggatcat ttttctttat
     1561 cagattgatc tctaagagca atgcttcgat attcgattct gtaacgatgt attcaaagtc
     1621 ttcgatttca ctaacaagcc gttccgtttt cgtattatgg ctgccagtaa aatatgagcg
     1681 gacacgattt tttaatattt tggctttgcc gacataaatg atcgttccat ttttatcttt
     1741 catcaagtaa cagccgggct gatcaggaag taaagctaat ttgtttttga ttcgttcgtt
     1801 catctggtac tccccttcat attcaattaa agttattata gcatgtctgt caaaaaaaca
     1861 atccagcctg acaacgtaat tatactcaaa aaatattaat ttgaataacc ttaactcaac
     1921 cgcaaaaaaa ccgaggacgg aactggattc agtcccaccc tcagcttatt tttgctgtaa
     1981 gtttttataa atattgatcg atcatttgac gtagttgttc ttttgaatga acaccaacag
     2041 ctttttctac tacttgacca tcttttttca acattaatgt tggaatgctc atgataccga
     2101 attgttgagg agtttctgga ttttcatcca catccatttt agcaattctg aattcttctt
     2161 catcatattc ttgttccaac tgatccaaaa tcggtgcttg catacgacat ggaccacacc
     2221 aagttgccca aaaatcaatc aatacaagtc ctttatctgt ttcttcatta aatgtcttat
     2281 ctgtaattac ttgtgacata tcaaagacct ccatttttaa aatctatgat tagtatagca
     2341 tttctatact tggatagcga accatctgct tacctttagt aagctttact taaatttaac
     2401 aattgtcgca ccatttccgc cttggtttgc cggtgcaaac tcaaagcttt tgacgctccg
     2461 atggttttta agataatcag taattccctt gcgaagtgcg cctgttcctt ttccgtggac
     2521 aatagtcact tgaggataac cagctagaat ggcagaatcc aaatattgat ctacctcact
     2581 tagcgcttct tcgtatcgtt ttcctcgaag gtccaattga ttcggcacgt gactactaga
     2641 ttcagatcgt acagcatgga taactcgttg tgtcggctct ttttgaggag caacaggcgt
     2701 cagctcgtct tcggacacat tcatttttaa tataccgatt tctacttgcc actgattccc
     2761 gtttttacgt aaaagcgtcc ctctttggcc gtaagtcgta accaacactt cgtctcccgg
     2821 tttcaacgtt ttttgttctt tcgccttctt caatacttta tttttcttca aagtttcttc
     2881 ttgatgtaga tttgctaatt gggtctttgc atcgatcaac tgatgctctt tcacatttcc
     2941 ttgcccaatc tgctgttgca ttttacgaat atcagcaatg attttttctg ctttttcttc
     3001 cgcctctgac acgacttcgt ttgctttttt ctttgctttt tccatttctt tttcacgttc
     3061 ttcaaagaaa tagctgtaag cttctttcaa atcatcgtga agttcttgtg cctcagaaac
     3121 aaaatgacgc atttccagat attcggtctc tgccattttc cggcgattct ctaaatctgt
     3181 gatcatttcg tttaaatctt ggctctcatc attcatcaac tgcttcgctt catcaataac
     3241 ttctgtatca agacctaatc ggctagagat ttcaaacgca ttgcttcgtc caggaacgcc
     3301 aatcagcaaa cgatacgttg gacttagtgt atccacatcg aactccatgc tggcattaat
     3361 cgtatttgcc cgattgtatc cataaacttt caattcagga taatgcgttg ttgccatcac
     3421 ataagcagac tttttaccaa gatcatctaa gattgcgatt gctagagcag caccttcttg
     3481 cggatctgtt cctgcaccga gctcgtcaaa taataccaga cttttttcat tgacttttga
     3541 taaaatatct acagtgttcg tcatatgaga agaaaaggtc gatagacttt gctcaatcga
     3601 ctgttcgtct cctatatccg caaatacttc ctcaaaaata ccgatttgac tttcttcacc
     3661 tgcaggaatc ggtaatcctg cttgccccat cagttgtaac agacctaaag tcttcaacgt
     3721 gatcgttttc ccacctgtgt taggtcctgt aatgacgatg gcttgatagt cttttccgat
     3781 tgtgatgtca ttagaaacta ctttttcctg atcgatcagt ggatgtcttg cttgtttcaa
     3841 gacgacatga ttgtcttcac tgattcccgg cacgatggct tttacttctt taccaaatcg
     3901 tgcttttgca ttcatcagat ccattttacc aatcacataa gcattgtgga tgatttcttg
     3961 acgatgggga acaagttcag cagaaagttc acttaaaata cgttgaatct catttcgttc
     4021 agcgatttgg tattgacgca aacgattatt caaatccacc acttgttttg gttcaataaa
     4081 taaagtctgt cctgaagcac tttgatcgtg gactacaccg ccaaatacac cgcggtattc
     4141 ttgtttgact gggataacgt agcggtcatt tcgcatggtg atgatcgcat cacttaaata
     4201 ctttgcattt ttcccacgga cgatcccatc taactgttca cgaacagctt gttcacttcg
     4261 ccgaatgttc tgtcttatgc tctttaactc aggagaagcg tcatcagtca cgcgtccgtc
     4321 ttcatcgatt gcttccttta aacggcggct caaaacaggg atcgtgacta actgatctgc
     4381 ccacatatac aaacgtgcaa attcgatttc actgtcagaa agatcatcaa tgaaacgatt
     4441 caattcgctt gtcgtagaca atactctgga aacttgtgca agctccactc cattcaagtc
     4501 tgctccgatc tcgatccgtt tcatatgggg acggatattt tcaagtttag gtatagggat
     4561 cccgcctcgc agacgttgca ctttcaatcc atcctcagtt tcttgaagcc agtttgtgat
     4621 cgtttgtttc tcactcactg gtaccaattc tgccagttct tcttttcctt gtgcagtcac
     4681 tacaaattgt ctgaccatct gtttgacttt ttcaaattcc aatgtttcaa ggattcggtt
     4741 attcatttta tcaccttatc tttcactatg aaaaaagagc tgtgccggac atacgctacg
     4801 cagccttctt aaataaacta ctctgtacaa aaaacgaaat attttccagc actattttcc
     4861 ttaattcaaa atattcgtta cccaaagctc atacatcttg ttggtaagaa atggcgttct
     4921 ctcaacgata aaacgagcga aactatgctc gccgaattga ttttggacca catctaatgg
     4981 aacaaaggca agtaactgca aaatcaaaaa tatagtaacg taagccatga ttagtgacag
     5041 gatgcctcct gcaatccagt ctacctgtct caagatcggc acatacgtca atccatggac
     5101 aaaaaccccc acaaatcggg taagcagcca gccaattagc aaaataatca gaaaagcagt
     5161 tcctgcataa aaagcttcat ctaggtgaaa agcaaatacc tgatcaaaaa atgctaactt
     5221 tgatgttggg gtgacagctg ggtatggaat gtataattcc aaatgacttg ctaatggttt
     5281 gtaaaaatgc tgggcagcaa taaatgacaa tacatatcca atcgcgaata tgatttgcaa
     5341 agcaaacccg cgacgtgctc cagaaaaaaa cgcaattagc aataaaaata aaatcagcaa
     5401 actcagcatg ctacgttcct ttctcttcta acttttttaa atttttctta ttttgataaa
     5461 aggatcttaa ttagaagatt tttgtttaat ttgtcctttt cgtttctcgt ttaaaatctg
     5521 ttgtgcttcc acatgatttt taatggtatg atctgcttga ccattttctt tcaatacttc
     5581 ccgagcttcg ttctcgattg cttcaatgcg tttcacacga ttttcaagct ctgtaaattt
     5641 gatcattttt ttctttaatt ctgctgtttc ttcttccagc tccaagattc tttcttgttt
     5701 cttcaattga tccgaaagcg cattgacagc catcaagatt gctgcttgtt cattatccat
     5761 ttgtggtgat aattgtttca attctgctaa ctgttcattg atcagcttcg ttacgatatc
     5821 catatgatgc ttggtttccc gtccgataat agtatatgtt tggttggcaa tcactgcttt
     5881 gtagcgtgtt ttttcgtgcg ccatacgaat catttcctcc tatttgatga aagttttatt
     5941 tagtgtacca tgaagttggt tctttcaccg ttctccacaa atatttttca catacttaac
     6001 tattatatga taaactttaa aaaaatgcta tgtctcgcaa tctcttagaa aacgaaaagt
     6061 cgtgagttgc ggtatagttt tatgtataaa aacgaggaga attttatgag caacgtcatt
     6121 atcaaagcaa caaacgacca acttagtcaa atgaaagcat actatcgctc ttatttgtta
     6181 aataaacaga ttccttatac tagctttgct gcaaaaaaag acgggacgac cattaccgct
     6241 tatacatccg gaaaagtgat gtttcaaggt acgaatgcag caatagaagc tgataaatgg
     6301 ggggaggcac aggataaaca agccaagcca gcaaaaacag cctcttcttt gccaaaaaat
     6361 atcagtcaat tatccgtttt aggtagtgat gaggtcggaa atggcagcta ttttggtccg
     6421 ctaacagtat gtgctgctta tgtgaaacgt gatcagcttg cagaattacg taaacttggt
     6481 gttagagatt caaaagagtt aaaagatagc caaatcatcc agcttgctga catcttaaaa
     6541 aaaaccatcc cctataaact gcttgtctta tctccagaaa aatacaatga gatccaacca
     6601 aaatacaatg ccgttcggat gaaagtcgct ttgcacaatc aagcgatcca tttgctgcta
     6661 aaaaaaatcg agccagaaaa accagaagcg attttgatcg atcagtttac gccggaagca
     6721 aattttaaaa aatatgcgcg tttagaaaaa aatcaattac aagaaaaact gtacttcgtt
     6781 actaaaggag aacaatatca tttagctgtt gctgctgctt ctatcatcag tcgtgccagc
     6841 tttttggaag agttacacaa agcatcagct gaagctggaa tcacccttcc atcaggagct
     6901 ggaacgaaat ctgaccaagt agcggcaaaa ctattggaga aaggcggcat gcctatgctt
     6961 gaaaaatatg ccaaacttca ttttgccaat acagaaaaag ctttgaagct gctaaaaaaa
     7021 taatggaact gtgacaaaag tcatgtcaca gtcccttgtt ccgaataaac ggtgttctca
     7081 tgccaactcc tcggtcacaa gtcacaatag tcatcaaaca tgagaagctg ttttttgaaa
     7141 ttgctccttg tgacgcggag ttaaacggca ggctcacaac ctcttcctta aacggtgttc
     7201 aaaagcttgc atctgcgata ataagcccgg ccaaacgaaa aatatgagga gctatttttg
     7261 tttggctggg cttatttatt actgaaaata agtggtcaat gcttgtgtca aacttgcagc
     7321 aatctgctgc tgataactgt ctgttactaa ttcctttaaa tcactatcat tattcatata
     7381 acccatctcg attaaaatag aaggttgaag attttctcga agaacttgat agttcccagt
     7441 ggagattcct cttgaatcca gaggcagatc agtgaaggag tcagcaattg tctgtgctaa
     7501 tgtttcctct tgtccatagt aataatatgt cgtgattcca gttgcttcat tcgcatattc
     7561 cgtagagtct gcgtgtaaac tgataaagac atctgccttt ttttcttcac tgtagtcgca
     7621 tatatctcca agctgtacta attcatctgt agtatgggtc aaataaaccg ttgctccagc
     7681 atcctctagc gcctttttta cttttatggc tgtacttaat gttatatctt tttcttctgt
     7741 ttgttcatca ttgcttaatg ccccagtatc atttccgcca tgcccaggat ctaatacaat
     7801 cactgcatca gatagctctt ccggttttgt ttgtatattc tttgtgactt tggttccatc
     7861 ttcgagggct aaatatccag tatagccatc atttgtcttg atctgataag cattttttgt
     7921 ttcagaaaga aactccgcca gctctccgcc gtttatagtt gcaagtgggg tagttagcga
     7981 gtcatctgta tatagtgtac ggtcattttt tccgattttg attaatgtct cttgttctgt
     8041 atcatcaaat gattcctttg tctttgtaac cgtactgttc gtactgctga ccgtttgtcc
     8101 aactatcttt ttttgaccaa aaacatctat ttgaaaacag ataataatga taatgattgc
     8161 aaatacaaaa gctccaatta tttttttcat tcttgactct cacctcgtag atccagacta
     8221 agggtcaaaa cttaaaatca gcttaagtac gcaaaaaagg ggctgtaaaa gatataaccg
     8281 tagaaaataa cattactgct aaaatgattt ttttcatttt tggcatatcc tgaaatctcc
     8341 ttttgactat tcgtggtgat tcatctaccc aataaatagt ttccttcatt ctacaaacaa
     8401 ttaatttctt cgttattaaa ttaaaacact tcctggaaca ccgacataat aaataaaaat
     8461 ataccctgat aatcatgaga gaataacaaa tttgtattta tgaaaaacta cctatataaa
     8521 aaaaacaaga tataacgatc ttcgtcatat cttgcttctt cacctaacta tttttctccc
     8581 tatataacag ctttacgctt ctcctaatac accattcaaa tataaatcga tcaattgcgc
     8641 cacatttagc ggttcttcca caggtctttt cgaccgagtg atgtaaggtg ctaacactaa
     8701 gaaaaaatgc cttgctgcga tttcttttga aatcccattt cttagcacag attggtttgc
     8761 tgaaaagatt tcttcaaaag gttcacgata atttttcttc catagaaaga aaagatgatc
     8821 tcttgtttcc tttgaaagat tgctttcaag atcgtgaagc atcagaaaca aatcgaccag
     8881 atgtgtctca atcaaaaact gcgccatttt tgtcaaggtt acttgaaatc ccgtttcgct
     8941 tgtttgaacg atttctctca aagcttttcc aacatctttt aagtggtttt ctaaaactgc
     9001 cgtatatagt ttttctttat caccgaaata atgatagaga ttgggttggg tgatcccaac
     9061 tttttgtgcg atctctcttg ttgaagtgcc gagatatccc ttggacataa acaagtcagt
     9121 cgcttctttc aaaatttttt gctgtgtatc tttgttgtat cgatggacca tttttccacc
     9181 tgctttttgg ttattctttt tctttcaata caccatcttc catcataaat attctatcgc
     9241 atttgtcaat cagccgtgta tcgtgagtaa ccatgatcgt tgctttattt ttgtcttttg
     9301 attcttttgc caagatatcg actacttctc gtgccttttc tgaatccaaa ctagcagttg
     9361 gttcatctgc tagaataatg gacggatcat tgtacaatgc acgagcgatt gcgacccttt
     9421 gccgttctcc accagatagt tcttctggat acttgttgat caacttatcc acgccaagct
     9481 gctcaaataa ttcttttgct tctttttgac gatgttcatg tttgatctta tctactaata
     9541 ataactgctt ttttactgtc agaaaaggaa ccagattgga agcttgcaaa ataaagccaa
     9601 tttctttaaa acgtagttta acgcgttcac tttctttttg attactgaat tcttgatcat
     9661 tgataaatac agagccactg ctaggcgtct gcaatccgcc tgccaaagtc aaaaaagtac
     9721 ttttacctga accagaagga ccgatgattg caacaaattc ccctttatcg atactcatgg
     9781 ttgtttcttt caatgcttta actacttggt cgccatcttg gaaacttttt tctacttctt
     9841 taaattctat tgctttcatg ctcttcctcc ttcttatcca attgccttca acggatcgat
     9901 ttttacgatt gtccgaacag aaaatagtgc gcctaacaca gctactacca gcatcaacaa
     9961 gctaatgacg ccaaaaaaca gccaattgct ttggaatgga accgcatcag gtagaacaag
    10021 ggatgttccc actgttccta atagaccaag aagaatacca acaaatgata aaatgaatgt
    10081 ttgtgccact acagaacgcg caataaatcc tcctgtgatc ccttgtgctt tcattacacc
    10141 aaagatatta atcttttgca tcgtcagtac ataaatgaaa atcccgatca caatagccgc
    10201 aataacgatc aaaaatccga tcataaatcc aaaagtcaat acttgcgcat tatatccagg
    10261 taattcattg atgaatttag aaattttgat ctgttctaaa tcttcaggta aattactgat
    10321 tttacctctg acaactattg cgttaatccg tgcattttca cttgtatctt ctttttcgaa
    10381 gcggatctct tggtaagcat tgatggttgt atagagaact ggtgagacat tgaatttggc
    10441 atgatccgta aagccagtta gtttaaacgt cttgtcacta ccagataact taactgtatc
    10501 tcctacggct agaccatatt cttctttcaa actaatatcg cctactgcct catcatcgtt
    10561 atcaaaagct tttccttcaa caagattggg catgatgaat tgatttttat cgataccgaa
    10621 gaagctaaca ttgatttttc cttcttctgt actgtcttta ctagtcacga ctccaggtgt
    10681 ctgggcaaga tacgccactt tcccaccttc gacttcttct gctatcttct tagtaatcat
    10741 ggacatccct agatttgaat ttgcatcttt cgaaagcaca atcgaatctg cctcccactt
    10801 gtcgactgct gttcgattat cttgagctaa gccataagct aacccagtca aaaaaaatac
    10861 caggtaggcg atcaaaaaca tcacccctgc aactaacgca taacgtaact ttgaatgcat
    10921 aatttcattt aatgctaaaa acatttaact cctcctcctg aaataatcat tttatacaac
    10981 aattttatca cttgataaat ttttggcgaa tattatgctc aaaaaattct tccgctattt
    11041 cattatatga attttttatt ctcgattgat taatatttat tatatcccca cacaataaaa
    11101 gaccgtgata tttctcacag tcttttatct actctattgc tatttgctct cttagctagt
    11161 actttaaaag ctaactcatt gaggtagtga aaacgagagg tgtgttcaga cagaaatcca
    11221 aaatcctcaa ctgctagctg tggaatcgta gttttgtgtt tcacgatatc cgcaagcagg
    11281 ttcaaatctg ataataagaa ttcttggctt atcagctgtc cgccaattat ttctaatgta
    11341 tccggatggt agatcagctt tgcatttact gttttgtttt cttcatcgta ttgtggaaat
    11401 agatcaaatt ctcctgaaca cgaaccggta ttttgttcca agaatggcgc ttcatcttct
    11461 gtcagcccta cagttcctaa aaagtagtct ataatgactg ctgctgttat cttttgtctg
    11521 aggtcgtaag cggttggctg accactcaaa gttagcgcta ctgctcttgc catatgtaca
    11581 gctcgagtaa ctaaaggtaa aaagagatgc cgtttatttt tgtcaaaact gactgggacg
    11641 atatctccaa tcgcatagat gtccggatca cttgtctgca gatattcatt gactatcaca
    11701 gtctcatcct catacaaagc cactttttct tttaacaatg tcgtattagg acgagtttgg
    11761 gcagaaaaaa tgaccatatc tgcctttatt gcttgagata gaagttgtac ggatactact
    11821 ttctcttctg attttttcca atcgatcaag aattcatttg gataaaaatt gatttgctta
    11881 gtttgcagct gattttgaaa actgttgatc atatccttat cgaaatatct tgataaaaca
    11941 ttcggcatat aatcaactag atgaataatg tatcctttat ttctcaaagc atctgctaat
    12001 tccaatccaa tataaccagc accgaagatc actacttctt ttgcttcttt taaagcggca
    12061 agtacttgct tagattgcac tagattttta taggtgtaga cattttcagc ttcaatttgt
    12121 ttatacaagg tgacaggaga ggaacctgtt gctacgatta atttatcata ggttatggtc
    12181 tcgttttttc cttctttgga tgcgaggatg agctgatgtt tgtctggatc aagttcagtt
    12241 actctccatt cggtgatcag ttttgcccca ctttcttgaa gagtccgcat gttatgggca
    12301 acttcagcta aactacgtat ctttcctttt aaataaaggt tgattccatt tgaaacatag
    12361 cctagtccat ctttatgatc ttcatcgatc agtaaaactt ctgctttcgg ttgaagtttt
    12421 cttagattta gggcagctgt gatgcctgca tgggaagcac caatgatcac gattttcatg
    12481 ttttattcat ccttaaattc aatttttcta aataacattc ttttaaaaat tggttcaaat
    12541 aggggaaatc ataattatat tcgttgctag tgaatacaaa aactttagcg gacttctgtg
    12601 acgggtactc tttcaattgg ctagtcaaaa tgatatcata ctgcttttct ttctcgtatt
    12661 cgactaaatc atatttgatc gtattcagca attgttcctg gatgatttct ttcacgatat
    12721 ccgccattag cctttcataa ggaagatcat aagcaatttg cagacgtaat tctaactgct
    12781 tgatcagcgt ccgcaaaata gatgcatagc gagcaaataa ttcaaagcgg gcattttcct
    12841 cgtctacttc agtaaaatca acaatagcaa atgtttgtct gaccaatctt tcacataaaa
    12901 ccaggaattg tttatcttct acttcagtta accgctgttt taatccttct tggccaaaaa
    12961 tattcatcca tcctctaaaa taggcaaaat gagcgtgatt ttgcatcact gtcaacgcaa
    13021 tcttttgcga aatctcttct ggaaaaacat aaaaagagaa atattcttct atcgattgtt
    13081 taaacgttcg attcaattga gctactacgt cgtctttgta tttttcgctt atcaaaaact
    13141 cctggacatg cgagttatgt gtatctaatg taaagttact gatcatgaaa agataaaaaa
    13201 ccaacgtttc attttgattc caaggaaagg catattgaaa gaagtaacgg gtcaaaaatg
    13261 tatggaggtc caataaaaat tgtgtcgttt gaaacaatcc aacaggaaga ttgctataat
    13321 ctggtttgga ttttttcgta cagcgcattc gcttcttcat aatggaaagc catatttcaa
    13381 atttcaatcg tccttcttct gtaatagtaa aactaaactc tttttgcagc agatctaaaa
    13441 aagtgtttcc tgataaatca ctgtattctt ttttgttttc aagataagga cgtccaaacc
    13501 aaaataattg gaaataaaaa taacaaattt gactttctgg accggtcagc cgcccgcttt
    13561 ttattttcag atgaaactca tttaaagaat catttaattc cttcactctg cgagacaaag
    13621 tagccgaact gatattaagt tccatgacaa gtttctgccg atcaaatgag ccattttcca
    13681 acagataaag aagtatctga tattttgtag aatagcgcaa gtagcaatga taataaacat
    13741 cgactgctgc tatatttcca tgtctgatta ataaaatatt cccttcttgg ataacaattt
    13801 ccaattgttt gatattttcc aagttagctt gtaaataaaa cagatcttct ttcaaggtag
    13861 cccgatcaac tgataagcgg gtaagtacgt tattaacgga aatctctcct ttttcctcta
    13921 ataaaagttg gaaaagtttg atttgccgtt gttcattttt ttcaagcaaa ctgaaaagtt
    13981 ccaagtccac tcctccaagc atttctgatt ttaacagcag aagtttcctt ctcatccttg
    14041 aaaaagagga tctgtgacag ttatcacaga tccatacaaa ttctaatcac ttagctggtt
    14101 ggcccagctg tcagttccca tgtgaccatg ccttgatacg ttgcgccagg ttgagcattc
    14161 gtactgctat ttacctctaa gagtaaacca tcggtatcgg gaaagtcaac gagaacctct
    14221 cctttagctg ttccatcact ctcataaatc aacgtgtttt gaccttgctg aatcaattgt
    14281 tttgatccag aatgattgaa gtataatttt gttccgctta attcttgacc ttgatcattc
    14341 ttaaataaag aagtcaatgt ccctgtcagt tgccaaggag acgtgcttgg aagccgagta
    14401 tcccgcacat caaaagaaaa atcgcctttt gcttcataat aacttgtctt caacggtttg
    14461 gggagatcgt taccaaatgt aaaatcatct ggtacattaa ctaatgttaa ttcttgtttg
    14521 gggacaagaa ttgttttagc agatgtttgg acaactgggt taggaagctt agcaccatct
    14581 gctaatgtaa tattgctgga aagtacagct gcctttgtca gtatgctgac ggattgtaca
    14641 gaagtatcta cagtatttaa ttgatagttg taagtaagat gaatggtatc tcctattttg
    14701 ctcaatagat ctgctgggac agaaactcct tgttgaatgc cggtatctgc gatagttgta
    14761 ccatttaatt ttaaggaacc attgatataa gtagtatttt caggaagtga gatcgtgatt
    14821 tttttgtcgg ttatcgcatc cccagatttt gttaacgtga atgtcacatt ttcttgaatc
    14881 gtatctcctg atagagcaga caggggatct cctgtctcag gttgtctgct aaggtttttt
    14941 agctgactat ctgcactcac agatggaaaa ttagccactg ttatttgagg aagagtggtg
    15001 attccagctt gttcagactg ccatttgtac gcaaaagacg gatacatgtt agtaagtaca
    15061 tcgtccacca agcgataatc aaaataatat tgccctgaag ccaatgcagg tagtgtagaa
    15121 gtagctgtat ttacaccccc gttatttgat tggatcgttt ggaaaagttg atctgtccat
    15181 cctgtcgcta atttttgggt tcctttcgaa taaagcctgt aatggatcga accctttttt
    15241 agttcagttg ctgaccaatt ggccgtgact tggtacgatg cagaagaaag gagctttccc
    15301 cactgactat cggctgttaa gtttgttggt aaaaccgttc cgtctagctt gtactgctcc
    15361 aataccttta aagcaacagg agaaagcaat ccgccttgcg catctttccc actgtacacg
    15421 atattggctg tctgattcaa tccacttgtg cttgctggag tatgagtcag tattgctcct
    15481 tgtgtaacgt tttttcctgt aaaatagttg actacgcgat caaagcgact aaatggcaac
    15541 tgatcaaaac gtccttcagt cagccccatc gtcaatgtcc gactattggt agagcctgga
    15601 aggttctggt tatttccatc cacgtattct gtggtatcag caaatgtatt atattttcct
    15661 gttgaatcac ttgttgagat gtaactattc cacgtattta tctctgcact tgcagaaaat
    15721 gcatatgctc ctggattcaa gtttccgcca gttaatttag gataaaatct acctgcagct
    15781 ggagctaaaa tcgaattgaa catacgatct tggataatat tattcgaatc ataaaaatca
    15841 acagaaaaaa cggccttttt agacatagaa ggttgtaatt tacccttggt caaaattttg
    15901 tttggactcg tatcatattt atctccgttt tctgtcatct tcgcgacatt agctgtaaca
    15961 tcaaattgaa gcgggatatc gtccacgttg aattcgcctc gcgtaataat agcaataata
    16021 tctgccaata gagtatttgt atcccacgac cccatcccaa aacgcaaggt atggcttcct
    16081 gctgataata aactaccgct agtaaccttg atgggggcac cggcaatata caagtaggcg
    16141 ttgttccagt caattgcttt taatacatca ctgcttgata catgttccgg caaattgatg
    16201 atcgccacaa atgcaaatcc ttgtgcagtg atattcacca aatcaagtac atttgttagt
    16261 ttcacatggc tttcccaatg aaaacggacc gtgccacctt tccaaggatc atttgaacca
    16321 tcgttcaaat aagtagcagt caatttaggc aattggttag ccccagttag ttggacaagc
    16381 ttcccgccat tgactgcact acgtccacca tttggaggta cgatattttc atacgtgtat
    16441 ggatcataag aaatggcagg aggcgttgct ccaacagctt ctgccgaagg tgaaaagata
    16501 aatatactta aaacaaaaag cgaagtaaaa aagaaggagc gaaaaaatga aaatttattt
    16561 tgtagcaaca tgttcatcct ccttttcctt tcttctccat aacaacaacc acgcaagaag
    16621 ccataattct ataccaatga tcaaataaag aaaacggatc ttttcttcat tcgtctccgg
    16681 gaatacgcca tgttgattag attctttata aatcggtagc tgtgtctgct gttttgatgc
    16741 gccattgact gaaccacttg gatgcggaac tggacttaga tcataaaatg taatcgtacc
    16801 tgaacttttg gtatctgctt ggactggctc ttgccagctg aagaacagca aacttgcaat
    16861 aatccctccc aataagatcg atgttttcaa cgaaatcacc tcatcatgtt gtttctcctt
    16921 ttttcttttt cttgtggacc cacaaaaaca gcaaaatcaa ccataacact agaactgcag
    16981 caaccaatcc tgcataaagc catgggaaag attgttcttg tactgcaaaa acggaatttt
    17041 catttacttt ttgtgctttt tcttttttga tcgtaaagtt ttgtgtaaat cgccattcat
    17101 tactgtcgcc tgattttgct atgatttctg cgcggtaatc gccggctttt gctttatcac
    17161 cagctaaatc aagattcaag tgaaaattcg aatttggtgc aactaaaaat tcattagacg
    17221 tattttcaat ataaagctga ttttcataaa agatcttgct cgtaaatatc aaattgctga
    17281 taatcctagg ctgaggattt tggaaacgaa gctgaacagt tggctttgcg ttgacctcgt
    17341 ccaaatctcc tgataaaagt ttcagatccg gttctatcgt ctcttgactt tcttgtaaaa
    17401 cgactgcgag attataagca atacgatttt tgatttgtgc tccttctgta gcattttctg
    17461 tttcagcttc ttctttaggt gaaacatgaa tacccgctaa tacacgtccc ttaaagcttt
    17521 ttgctgggat tttcacttct ataggtatag ttgtttctgc atgtgctgat acattgattt
    17581 cctttttcgc aatgagcaaa acgtcacgga catcaaacgg caatgtctca tctaacgttg
    17641 ggtttttttg actataatcg atcgttccac catcactcgt taccgccgga ttgacagaga
    17701 cctcaaatgt tcgttcttca tctgaagaat tgctgataat tacttctagt gtctgatttt
    17761 gttccggtgc cactaataaa tcaaaataac cgattgcttt atctatttgg ttctctggaa
    17821 ataccggttt gattccaaag tctcctgctg aatcctttgc ttggctgatt gacggaaaaa
    17881 tgaaaaatag acttaaaata gaaaaaaacg aaacaaacag tcgtttactt tgattcattg
    17941 tccaccacat cctttcaaca acaactgcgg taaaaaatcc ttcacctata gtattttcac
    18001 cgcagtttgt tgaatttatt tcatactcta tgctactggg ccagccacga ggttccaagt
    18061 caaatcagcc gtgaatgttc cagctgctgc tttcggcact ttcagtgtga tatttgctgg
    18121 atcataaaca gaaagccatg ttcccagacc ttgatctttt gctgctgtaa atattggtgt
    18181 cgctgcactt gctttactca acccagttac agtttgacta gttggtccag tggattcgtt
    18241 atctgacgta cttttaacag tggatgttcc aaaatctaat tcagcgcctt gcaaaacttg
    18301 tgaaccgttt acaaaatctg aaaccgaaac tgtcacgttc cacccttgtg cttggccatc
    18361 tgcacctaca cctcgacgat ctgatacttg gagataaggt gtatcgtttt ttgaagcgtc
    18421 tacttgatac gtttttgttc cgctttcaat ctcgtgtgta ccaaatggca attcaggaac
    18481 tacatccaat gttaaagaac ctgtctggcc agttgctgga ttttccggat ctgctggctc
    18541 tactggtttt gaaggatcag tagggttaag aggctcaacg gtatttgttg gttcagtaaa
    18601 ggtaatatct ccttttgaag tcgcttgacc ctcagcaaaa gctggtgttg ccaagccgcc
    18661 tagaagaagt gttgaaagta ataatgtccc aatcatgttc tttttcataa taaatcactc
    18721 ctttttctta gtgacctaag tatagtagag acagtatgca aaaaatcctc atgaattcat
    18781 ccgcttactt gaaattatga ccctttttgt ataaaagaga aaaaaacaat aaaatcgttt
    18841 acatgaatat tcttcctttt ttattaagta tgaaacaata tttcaatttt attttttcgt
    18901 ttcaaattat tctattagtg acatccttga actataaaaa taggagtacg gcatcatctg
    18961 ccgcactcct atttttttcg tcatttattt cttaaataaa acgattctca cgcaatttta
    19021 tttttttgat atcttctact gtttgtgttc cagctaattg catgaccatt tccaattctg
    19081 ttttaaagaa atcaaacact tgatggactc ctgtacttcc tcctaaagac aatccataaa
    19141 taacgggtct gccaatagca acaagatcgg cacctgatgc gattgctttg aaaacatgtt
    19201 gtccacgtcg tacaccgctg tcaaacacga ttggtacacg tttatctact gcttctgcca
    19261 catattgcaa cgaatcaaac gcagcaggcc ccccatctaa ttgacggcca ccatggttag
    19321 atacccaaat tccaccagca ccagattcta atgatcgata gacatcttct tctgattgaa
    19381 cacctttcac gtagactggc aaatcagaat gagcagcgat aaattctaca tcttttggac
    19441 taagtttttg tttggaagat ttatatacag catccatcgt ttggcctact cctgattgat
    19501 aagcctgaac gatcggcatt ggcagcggga aggtaaaccc gtttctgcgg tctgtttcac
    19561 ggtttccgcc aactgttgca tccgctgtca atacgattgc ttttgctcca ttacgcttgg
    19621 ccacttcaag aatatccaaa ttgattccat tatctttgct catataaaat tgaaaccatt
    19681 gcggtgcttt ctccccgcct gcttctctta tctcttctaa agtacaagaa gcgtaagaac
    19741 ttgctgtata gatcgtccca aaatctgcta caccttttgc agaagctttc tccgccttca
    19801 catgagctaa tccatgagca gcaactggtg ccatgatgat tggtgcagtc agcatttctt
    19861 cgtcaaaatg tgtagttgta tcgggtaatt ctacatctct taatacatga ggaataatca
    19921 aacgatgatt gaatgcccgt tcattttctt gataagtaaa taagtcgcca gcaccactgc
    19981 tgatgtaacc ataaccacca gctggaatca cttgtgaagc cgcgatttct aaatcttcca
    20041 tattgataaa atctactatt ccttcatttg tacctgcttg atatgttttt tccatctcaa
    20101 tgccttctct ctataaaatt tgaatgatga acaatatgat agcgtttaca ctttttatat
    20161 tactcttcga cattgataca ggcaaataat ctgtttacat ttcagccatt ccagatgtca
    20221 ctactctgga agaaatgatt gtaataaaaa tagataaaaa accgatggga ctttgacaaa
    20281 agtagtgtca aagtcccttt ttttgaataa acggtgttca aaagccattt tctcggtatt
    20341 aagtcaaatt tatgcaaaaa tgtggaaagc cattttcaca aaatttttct tactactcga
    20401 aactaagcga ctttttcaca acctctcgat
//
LOCUS       DAMEET010000052        20301 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.053, whole genome shotgun sequence.
ACCESSION   DAMEET010000052 DAMEET010000000
VERSION     DAMEET010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.053"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..683
                     /locus_tag="PB780_001667"
     CDS             <1..683
                     /locus_tag="PB780_001667"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDE0733721.1"
                     /translation="LTSDENLIWIEYLDQATALNGARKDQISGKGELNNQITSAIFRY
                     LTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFSKRLGVPEGKEFIKPII
                     EFYYKDDQLDDPFINEEHIYFLQLAKSEEISELKAQALAINEILTELFAKVGLVLVDF
                     KLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDLGDLIPVYQEVYNRLSE
                     LTEEKERV"
     gene            683..937
                     /gene="purS"
                     /locus_tag="PB780_001668"
     CDS             683..937
                     /gene="purS"
                     /locus_tag="PB780_001668"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HDE0733722.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            939..1610
                     /gene="purQ"
                     /locus_tag="PB780_001669"
     CDS             939..1610
                     /gene="purQ"
                     /locus_tag="PB780_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HDE0733723.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            1611..3833
                     /gene="purL"
                     /locus_tag="PB780_001670"
     CDS             1611..3833
                     /gene="purL"
                     /locus_tag="PB780_001670"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HDE0733724.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            3818..5257
                     /locus_tag="PB780_001671"
     CDS             3818..5257
                     /locus_tag="PB780_001671"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDE0733725.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            5280..6332
                     /locus_tag="PB780_001672"
     CDS             5280..6332
                     /locus_tag="PB780_001672"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDE0733726.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            6329..6907
                     /gene="purN"
                     /locus_tag="PB780_001673"
     CDS             6329..6907
                     /gene="purN"
                     /locus_tag="PB780_001673"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDE0733727.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            6904..8439
                     /gene="purH"
                     /locus_tag="PB780_001674"
     CDS             6904..8439
                     /gene="purH"
                     /locus_tag="PB780_001674"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDE0733728.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            8455..9729
                     /gene="purD"
                     /locus_tag="PB780_001675"
     CDS             8455..9729
                     /gene="purD"
                     /locus_tag="PB780_001675"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDE0733729.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            9851..10765
                     /locus_tag="PB780_001676"
     CDS             9851..10765
                     /locus_tag="PB780_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDE0733730.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(10898..12463)
                     /locus_tag="PB780_001677"
     CDS             complement(10898..12463)
                     /locus_tag="PB780_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDE0733731.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSYFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(12456..13907)
                     /locus_tag="PB780_001678"
     CDS             complement(12456..13907)
                     /locus_tag="PB780_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDE0733732.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(13921..14937)
                     /locus_tag="PB780_001679"
     CDS             complement(13921..14937)
                     /locus_tag="PB780_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDE0733733.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15157..16245
                     /locus_tag="PB780_001680"
     CDS             15157..16245
                     /locus_tag="PB780_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HDE0733734.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            16299..17825
                     /locus_tag="PB780_001681"
     CDS             16299..17825
                     /locus_tag="PB780_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDE0733735.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            17827..19032
                     /locus_tag="PB780_001682"
     CDS             17827..19032
                     /locus_tag="PB780_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDE0733736.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            19289..20266
                     /gene="guaC"
                     /locus_tag="PB780_001683"
     CDS             19289..20266
                     /gene="guaC"
                     /locus_tag="PB780_001683"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDE0733737.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
BASE COUNT         6488 a         3452 c         4657 g         5704 t
ORIGIN      
        1 atcttacttc tgatgagaac ttgatttgga tagaatattt agatcaagca actgcgttga
       61 atggcgcgag gaaagaccag ataagtggaa aaggagaatt gaataatcaa atcacaagtg
      121 caattttccg ttatctgact caaagaggaa taaaaaatca ttttattgaa caattgtcta
      181 aaaccgaaca attagcaaaa aaagtacaaa ttatccctct ggaagtggtt atccgaaata
      241 ttgcagcagg aagtttctca aaacgtctag gtgttcccga aggtaaagaa ttcatcaaac
      301 caatcattga attctactat aaggatgacc agttggatga tccatttatc aatgaagagc
      361 atatctattt cttacaactt gcaaagtcgg aagaaatcag tgagctgaaa gcccaagcgt
      421 tggctattaa tgaaattttg accgaattat ttgctaaggt cggtttggtt ttagtagatt
      481 tcaagctaga atttggccgg tcgaatggac aaattttgct tgcggatgaa gtaacaccgg
      541 atacttgccg attatgggat gaacgtacga atgaacatat ggacaaagac gtttaccgca
      601 gagatctagg tgatttgatc ccagtctatc aagaagtata taaccgtcta tcggaactaa
      661 ctgaagaaaa ggagcgagta tgatgtattt cgtaaaagta tatgtaacct ataaagattc
      721 agtgttagat cctcaagggg aggcagtaaa aaaagcagtc catcgtatgg gatatgaaac
      781 aatcgaagat atccgaatag gaaaatactt tgagatacaa cttgctgatg cagaagaacc
      841 aatcgataca gtgattgaaa cgatttgtga caagctgctt gcaaatgtaa atatggaaac
      901 ataccgatat gaaatcaaaa aaatggagga agtataacat gaaatttgcg gtcatcgttt
      961 ttccaggttc aaattgtgac gcagatatgt tatgggcagt tcgcgagatc atgggcgctg
     1021 atgccgaatt tgtacggcat gatgagtcct ctttagaagg atttgatggg gttttactgc
     1081 cgggaggatt ctcctatggt gactatttga gatgcggtgc gattgctcgg ttttctacaa
     1141 tcatggaaga agtgattcgt tttgctgaag aagggaaacc agtattcggt acatgcaatg
     1201 gattccagat attgacagaa gcaggacttt tgccaggtgt actgagacga aatgagtctt
     1261 tgcattttat ttgcaaaact gtaccattga aagtcaaccc caagacggcc tttacttcag
     1321 agtatgaagc cgacgaaata atctatcttc cagttgctca tggggaagga aattatttct
     1381 gtgacgaaga aacacttcaa gaactaaaaa aaaataatca gattgccttt acttataatg
     1441 acaacatcaa cggcagcgtg gaaaacattg cagggattac gaacaaagca gggaatgtgt
     1501 taggaatgat gcctcatcca gaacgtgcag tagaagactt gctaggatca gaagatggga
     1561 aacggttctt tgcttccatt ttaaaaaact tcggaaaggt gaccgtatag atgaaaacat
     1621 tgaaagaacc gacagctgaa gagatcaaga aaaaaagaat ctatgccgaa tggggattga
     1681 ctgatgaaga atatcgaatg atcgaagaag atatcttagg aagaatgcct aattatacgg
     1741 aaactggact gttttctgtt atgtggagcg aacattgttc ctataaaaat tctaaaccgg
     1801 ttctacgcaa atttcctact agtggacctc atgtgttgca aggccctgga gaaggcgcag
     1861 gcatcgtcga catcggtgat ggactagcag ttgtcttcaa agcagaaagt cacaatcatc
     1921 cttcagccgt ggagccttat gaaggagctg cgacaggagt tggcggaatc atcagagata
     1981 ttttcagcat gggggctcgt ccaattgcgt tgctagattc attgaggttc ggcgaattag
     2041 ataatccaag aacgaaatat ttactagaag aagtagttgc aggtatcggc ggatacggca
     2101 attgtatcgg cataccgact gtaggcggcg aaattgcatt tgatccttgt tatgaaggaa
     2161 acccattagt caatgcaatg tgcgtaggtt tgattgaaca caaacatatc cagaaagggc
     2221 aagccgctgg cgtcggtaat tcgatcatgt atgtaggtgc aaaaacaggt cgcgacggta
     2281 tccacggagc aacttttgcc tcagaagaat ttaatcaaga agaagaacag caacgttctg
     2341 ctgtacaagt aggcgatcct tttatggaaa agctgctgct tgaagcgtgt ttggaattaa
     2401 tcttgcatca ttcggattct ctaataggta tacaggatat gggagcagca ggacttgttt
     2461 catctagtgc ggaaatggct tcaaaagctg gctcaggact tatattgaca ttagatgaag
     2521 tcccacaaag agaaactggg atgacacctt atgaaatgat gttatcggaa tcacaagaac
     2581 ggatgttgat ttgtgttaaa tcaggagagg aagaaaaaat ccaagaacta tttcagaagt
     2641 atgatctaga tgcagtcact atcggtaaag taacagacga cgggcaatat cggctatatc
     2701 atcaaggaga agaagtagca aatctgccag tcgatgcatt agctgaagat gcaccagttt
     2761 atcataaaga aatgaaagaa ccagctcgaa tcgtagaatt tcagcagtta gctccttatc
     2821 agccagttat cgaagaacct ggacaagtat tgcttgatct gctgcagcaa ccgacgattg
     2881 cttcaaaacg ttcaatctac gaaacttatg attcacaagt acaaaccaat acagttgtcc
     2941 gtccaggaag cgatgctgct gtgatgaggg ttagagggac gaataaagcg ttagctatga
     3001 ccacagactg caacgcgcgc tatctgtatt tggatccaaa aatcggagga caaatagcag
     3061 tggcagaagc tgctagaaat attattgcta gcggtggtaa gcccttggcg atcaccgact
     3121 gcctgaacta tggttctcct gataaaccag aagttttttg ggaattatcg acttcagctg
     3181 acggaattgc tgctgcgtgt gaaacgttag gaactcctgt tatttcagga aatgtctctt
     3241 tatataatga aacggatggt caagcaatct atccaacacc aatgattggt atggtcggat
     3301 tgattgagga tcataaacat atcactactc aggaattcaa aaaatcaggg gatctaatct
     3361 atatattagg taagacgttt gcagattttg atggcagtga attacaaaag atgcagcttg
     3421 gaagaatcga aggtgtgatc cgaaactttg atctatccat tgaaaagaga aaccaagagt
     3481 tagtattgac agccatccaa aacggattga ttgaaagtgc tcatgactgt tcagaaggtg
     3541 gcttggcaat tgcactggca gaatcagctt tcaaacatca gctagggata tctgtacagt
     3601 ttgaattgtc ctctgctcaa ttatttgcag aaactcaatc aagatttgtg ttgacagtcg
     3661 caccggaaaa taagacacga ttcgaagaaa tgatgggaga tgctgctgtt ttagcaggta
     3721 aggtgacgga tgaagcgata atcgaaattt cagcaactga cggtcagatc aaaatagaaa
     3781 ctgcagtagc aagaaagtgc tgggaggatg caatcgtatg tcttatgaag tgaagagttt
     3841 aaatgaagaa tgcgggatct tcggtgtttg gggtcatcca gacgccgcta gtgtggcata
     3901 cttcgggctc catagtcttc agcatcgagg acaagaaggt gcaggtatcg tgtccaatga
     3961 tcgaggaaag cttaaggggc atcgagattt ggggctgctt tctgaggtat tcaaagatca
     4021 aagaaaattg cagcagttaa caggagaagc tgcaatcgga catgtacgtt atgcaacagc
     4081 aggcaatgga agtgtggaca atattcagcc ttttctattc aaatttcatg atcagtcgat
     4141 cggcttagcg cataatggta acttgatcaa tgctcgttcc cttagaaaag agttagagga
     4201 aaatggggcg atcttccatt ctaactccga tacggagata ttgatgcatt tgatccgacg
     4261 aagtgaagaa acagattttt taaataaatt aaaaagtgct ttgaatgaag tcaaaggtgg
     4321 ctttgcctat ttattaatga cagaaacggc aatgattgcc gctttggacc caaatggttt
     4381 tcgtccatta gcaatcggac aaatgaaaaa cggagcatac gtgattgcct cggaaacttg
     4441 tgcattagaa gttattgggg cacgttttat aagagatgtc cagcctggtg agatagtgat
     4501 catcaatgac gaaggaatcc agattgattg ctttacgaag gacacgcagc tttctatttg
     4561 ttcgatggaa tatatctatt ttgccagacc agattcaaac atcgcaggga tcaatgtgca
     4621 tactgcacgg aaaaatatgg gacggaattt agcgaaagaa tcgccagtaa aagcagatat
     4681 ggtcgttggc gtacctaatt cttctttgtc agccgcaagc ggttatgcgg aagctagtgg
     4741 aatcccttat gaaatcggat tagtaaaaaa tcaatacatt gcccgaacat ttatccagcc
     4801 aacgcctgag ctaagagagc aaggtgttcg aatgaaatta tctgctgtac gaggagtagt
     4861 ggaaggtaaa aaggtgatta tggtcgatga ttcaattgtt cggggtacga caagcagacg
     4921 gatcgtccat cttttgaaag aagcaggggc aaaagaagtc catgtgagga ttgcttctcc
     4981 tcctttgaaa tatccatgtt tttacgggat tgatattcaa acgcgtaaag aactcattgc
     5041 ggcaaatcac acgattgatg aaatcaaaga atgtatcggt gcagattctt taggtttttt
     5101 aagtgaagag ggtctgatcg aatcgatcgg tctggaaaaa gatgctccgt attcaggatt
     5161 gtgtatggct tatttcaacg gagattatcc gacgccgtta tatgactatg aagaaaaata
     5221 tcaggaatca ctaaaagaaa aagtatcgtt cttttaaaag cagaccaaga agggagtata
     5281 tgaattcaat ggcaaatgca tacgcaaaag ctggcgtaga tgtcgaagct ggatatgaag
     5341 tagtagaacg gatcaagaag catgtcaaaa aaacggaacg tttaggtatg atgggcgcta
     5401 taggaggatt cggcggttgt tttgatttat cggtctttca attaaaagag ccagtccttg
     5461 tctcggggac cgatggcgtg gggacgaaat tagcagtggc aatcgaagca gacaaacacg
     5521 atacaatcgg gatcgactgc gtagcgatgt gtgtcaatga tatcttagca caaggcgcag
     5581 agccgttata tttcttagac tacttagcaa cggggaaaaa tgatccgtta cgtttggaaa
     5641 atgtagtaaa aggtgtagca gatggatgtg ttcaaggaaa tatggcgctg attggcggag
     5701 aaacagctga aatgcctggt atgtactcag gtaatgatta tgatctagcc ggatttgctg
     5761 tagggatagc tgaaaaatct caattattga caggagaaac gatcaaccca ggagatgtgt
     5821 tgcttggaat agcatctagc gggattcatt caaatgggta ctctcttgtt cgcaaaattt
     5881 tctttgagca gcagcagttt tctttatcat caaaactgcc agaattgaaa aaaacacttg
     5941 gcgaagaatt gcttgagcca acgaaaatct atgtttcttc cttgcttccg ctgatcaaag
     6001 aaaagaaaat aaaaggagct gcacatatca ctggtggagg gtttgtagaa aatatcccgc
     6061 gaatgttacc agaatctatg gctgcacaaa tcaaacttgg ctcatggccg attcttccaa
     6121 tattcggctg tttacaaaaa tatggtgaaa ttcctgagat ggagatgtat gaaatcttca
     6181 atatgggcat cggtatggtc ctggcagtcg aagaatcgaa agtaaaagaa atagtacacg
     6241 aaattgagca agctggcgaa aaagtttatg aaatcgggaa agtgatcgcc aaagaaaaac
     6301 atcgtgtggt gtttatagga gaagaacgat gagaatcgct gtttttgctt ctggaaacgg
     6361 aagcaacttt caagcgctag ccgattatct atcgaaaaaa ggattggaat cttcgattga
     6421 ctggttgttt tgtgatcagc ctgaagcata tgttttgaaa cgtgcaactg cgttgagtgt
     6481 accggcagat tgtttttcac ctaaagaatt tgattcaaaa aaagagtatg aagaagcaat
     6541 tttgcataaa ttgaaagaaa aaaagatcga tttgatcgta ttagctggat atatgcgaat
     6601 cattggaccg gtattgctga aaaattatga taaaagaatc atcaatatcc atccgtctct
     6661 tttacctgcc ttccctggtt tgcatggcat acgtgatgcc tttgaagctg gagtaaaaga
     6721 aacgggtgtc acaattcatt atatcgacca aggtgtagat actggaccaa tcatccggca
     6781 ggaaaaagtg agaattgaac aggaggacac gttcgattct ttagaagaaa aaatccatag
     6841 agtagagcat cgaatctatc cagaagtgat tagtgaaatt attgaaaatg gaggctatct
     6901 gaaatgacaa gagcattgat cagtgtatca gataaaactg ggattgtagc attcgcccaa
     6961 ggtttgattg aacaaggagt agaaatcatt tcaacaggag gaacaaagca tgcgttggat
     7021 aaagctggga tcaaaacgct ttcgatcgaa gaagtgacag ggtttcctga aatgatggat
     7081 ggccgtgtca aaacacttca tcctaaaatc catggtggtc ttttaggaag acgcgatctc
     7141 cctagccata tggaggcaat ggaaacggaa aatatccaac caatcgactt tgtttgtgtc
     7201 aatttatatc ctttcaaaga aacgattatg aaaccagatg tcgaaatagc agaagctatt
     7261 gaaaacattg atatcggtgg tcctagtatg ctccgtagtg cagcaaaaaa ccatgaattt
     7321 gtcacctctg ttgttgatcc atctgactat gaagaagtac tttcagaatt acgtcagtat
     7381 gggcatactt cactttcttt cagacaaaaa ctagcggcaa aagtctttcg tcatactgcg
     7441 gcatatgatg cactgatcgc tcaatactta acggattggg tggaagaaaa agaaccggaa
     7501 aaattgacac ttacgtatga acgaaaacaa acgcttcgtt acggtgaaaa tagccatcaa
     7561 gccgctgcct tttatcagtc cgctcttctg gaaaaatatt cgatcgcagc agcaaaacaa
     7621 ctccatggga aagaactttc ttataataat atccgagatg cagatgctgc gctacggatc
     7681 atgaaggaat atgacgagcc aacggtagtc gccttgaaac acatgaatcc ttgtggtatt
     7741 gggacagcat ctacgatttt agaagcgtgg gaagcagcgt atgaagcaga tcctgtttca
     7801 atctttggtg ggatcatcgt tttgaatcga gaagtagatc tgccaacagc agaacagatg
     7861 cataagctgt ttctagaaat catcattgcc ccttcttata cacaagaagc tttggaatta
     7921 ctgcaaaaaa agaaaaacat ccgattactg acagtagact ttgcgcagga agaaaatcaa
     7981 aatcgagaag aaacggtctc agtcttaggt ggacttcttg tacaagatca agatctggca
     8041 atagaaaatg aagaagagtg gaaagtcgtg acaaaacgta agccgactcc agcagaagca
     8101 aaagcattag cgtttgcttg gagagctgtc aaacatgtga aatctaatgc gatcgtactg
     8161 gcaaacgaac gtcagacagt aggaatcgga gccgggcaga tgaatcgagt cggttcagta
     8221 aaaattgcga ttgaacaagc gcaagagaaa atggaaggtg cagttttagc tagcgatgct
     8281 tattttccaa tggatgattc ggttgaatat gcagccaaac atggtataaa agcaatcatt
     8341 cagccaggag gaagcatcaa agaccaagat tctattgata tggcgaataa atacgatcta
     8401 gcaatggtct ttactgatgt tcggcatttt agacattaga gaggagtcgg ataaatgaac
     8461 attttggtga tcggtgcagg tggtcgtgaa catgcaataa gtaaaaaact tttagccagt
     8521 ccgaaggtgg atacggtgta ttgtgcgcca ggaaatccgg ggatgacaaa agatggtatc
     8581 aattgtgtcg ctatcagtga gcgagatcat tcagcgttaa tccattttgt taagaaagag
     8641 gctattgatt ggagtatcgt tggtcctgaa gttcctttgt taaatgggat cgtcgatgat
     8701 tttatggcgg aaggattgaa aatcttcggt ccgacaaaag cagcagctct gatcgaaggt
     8761 tcgaaaagtt ttgctaaaca attaatggat acttatcaaa tcccgacagc taaatccaga
     8821 agttttgaat gttacgaaaa agcagcagcg tatatcaaag aaaatggtgc gccaatcgta
     8881 gtgaaagcgg acggcttagc agctgggaaa ggtgtagtcg tggctaccac agtgaaggaa
     8941 gcgctggatg cggcagaaca aatgttgaac cagcatcgat ttggggatag tagcaaaaaa
     9001 atcgtcgtag aagagttttt ggcaggagaa gaattctccc ttttggcgtt tgtccgtaat
     9061 accaaggtgt atccgatggt catttcgcaa gatcataagc gggcatttga taacgataaa
     9121 gggccaaata caggcggaat gggtgcttat tcaccagtac cgcaaatttc aaaaaagatg
     9181 attgaaaaag ctgtggaaga agttttaaaa ccagcggctt tgggaatgaa agaactagga
     9241 cgctctttca cgggtgtttt atatgcgggt ttgattgcta cgaaggaagg accaaaagtg
     9301 atcgaattca atgcgcgttt cggtgatcct gaaacgcaag tcgttctttc tcggttgaag
     9361 agtgactttg cgcaagtaat cgatgattta ttagagaatc gtacagtaga gttaaagtgg
     9421 cagcaagaag gatacaatgt aggcgttgtg gttgcagctg ccggttaccc cgaagaatat
     9481 gagacaggaa tggttctacc agattttctg gaagaagagc tttctgttta ctatgctgga
     9541 gtttcctcaa ataacggcaa actagttgga gcaggaggac gtctttattt agtagaagca
     9601 tacggtgaga cgatcaaaga agcacagaaa aaaatttaca ccgcgttaat ggataaaacg
     9661 acagatggaa cattttatcg tacagatatt ggagaaaaag ctgccaatag cgaactgatc
     9721 gtgaagtaaa tcagctgctt ccatttataa aaaatacaac aaacataatc ggaagcgccg
     9781 attttagtga agccttttca taaagagtgt ggtacacttt tgttgtgtat ataaataagg
     9841 aggagttcag atgaaaaaaa ttcaattagg tacttctgat cagaaagcag ctgcagtgat
     9901 cttaggctgt atgcgtatca acaatgcgga agatccagta aaagtgattg agacagctta
     9961 tgaaaacggc attgattttt ttgaccatgc agatatttat ggaaacggtg aatgtgaaga
    10021 aatctttgca gatgctctag caaaaacatc aatcaaacga gaagatcttt ttattcaaac
    10081 aaaatgtgga atcgttccag gtgtgatgtt tgatttttct aaaaaacata tcattgaatc
    10141 tgtagaaaaa agtttgaccc gtctgaaaat ggattatgtg gatgctttac ttcttcatcg
    10201 tccagataca ttggtagaac cagaggaagt agctgaagcg tttgatgaat tggaacggtc
    10261 aggaaaagtt cgttattttg gcgtcagcaa tcaaaaacca atgcaaatcg aattgctgaa
    10321 aaaatatgtt cgtcaaccat tagtagctaa tcagctgcaa tttggcttga aacatactgg
    10381 aatgatcgat caagggattc atgtcaatat gacagatgaa gcaagtatcg atcatgacgg
    10441 tagtgtactt gattattccc gattgcatga tatgacgatc caagcatggt caccatacca
    10501 gtatggattt tttgaaggtg tatttatcgg gaacgaaaaa ttccctgaat taaaccaaac
    10561 attagaagaa ctagcagcta aatatcaaac gacaccaact ggcttggctt ctgcttggat
    10621 tttacgccat ccagccaata tgcaagtgat cgccggtacg atgaacgtag ggcgaatcaa
    10681 ggaaatcgct caagcttcag aaattaatat gtctcgggaa gattggtacg aattgtaccg
    10741 tgctgctgga aacattttac cttaaagatt gtcataagca ggtcaaaagg gctataaatt
    10801 aaaaagacag aataacttga aagctgtttt cagcttcgag atattctgtc tttttttatt
    10861 gcaaatggaa agagtatcat tgtacggatt tcgtttatta gttatttttg tttttaagga
    10921 aaagctgtgt ccgatattct tttggagaaa tcccttgttt tttcttaaat aaataactga
    10981 aatagttagg gtcattataa ccaatttcaa aagcaatatc tgaaatacga tagtctgatt
    11041 gctgcaataa tttttttgct aattcgattc tttgttctgt cagataatcg atgaatgttt
    11101 ggttaaacga ttgagaaaaa atcgtactga aataagaggg acttaatgca acttgttgag
    11161 cgacccaagt taaggacata tctggatcgg taaaattttc tttgataaaa tccaatgcat
    11221 ggttcaaaat tggctgatat ttgttccttg aaggctgtga gggcagagca gttaagaaag
    11281 aaatgaacat ataaactgtc gaacggtaaa gcgagtcgtc ggcgctgatt gctaagcgtt
    11341 gactggtgga tgtgcaatga accatttctt gcgggatttt cttttctttc aggcagacat
    11401 gctggagtag cttagtcaag acaaataaac gatataatcg tgttcgagtt ggtgtgcctt
    11461 gtggtttcgt caagtgttcg atgtacttag atacttcgtt tttcgataat gaagacaaat
    11521 caagtgagag gtcaaagtaa aagacagaat caactggaag tttcgcttct tgaatcaaat
    11581 cctcatagct gataatttgt tctttaaggg aactggcggt ttcaagtatc atctgcgtga
    11641 tctcaaaaga tgcaggtaat tcgctgattc tttctgcgac aggaccaatg gcgacagaaa
    11701 aattattttt ttgtcccttt tctaattcat ggaccaacgt ttgggctacc tgataactgg
    11761 tctcgatcac attttcttca agagaatcaa ctattaaaaa tttgatgaat cgagaggaaa
    11821 cagcagagaa gatcacttgt tgattctccc taaataaaaa cttcaaataa tcactgagat
    11881 gagcgtaatc ttcaaagtct gtactgactt ggtttgctgc taataatacg gtcatttttt
    11941 tccctgctaa agagcgattg aaagattcag caagtttcaa tgtttcactg atcgtaagtg
    12001 agcctttgaa caaaccgttc aagaaatgat tttttttcaa ttcgaaaaca agatcagaag
    12061 aagtgtgcgt ttctttttgc tgcaaatcta aagcagttac tgctttttct attgcttgtt
    12121 tcaattcgct acttttaatc ggtttaagca gataatcatc tgcctgtacc tggattgctc
    12181 ctttcatata atcgaaatcg tcgaatccac taataaatat gagacgagtc caaggaagta
    12241 gtttccttgc ttcttttgaa aaagtcaacc cgtccatgaa aggcatttgg atatcagtca
    12301 gaatgatgtc gggctgtaag tcgataattg aagacagtgc catctcacca tctccagctt
    12361 cgccgacata agtgattgga taataagaac caaggacagt tatttgttcc cgcaagttat
    12421 tgcgaatgag gtattcgtct tcgatgataa aaactttata catgtttgtt tcctccaatt
    12481 gttttgggag atagagggat catcagtgtg acagttgttc cttctcggta tttgctgtca
    12541 atcgtgatag gtttttgatc atttccgtaa taaagaagga gacgcttgtt tacgttgtaa
    12601 agtccgtagc cgatactgaa atctgaatcg atccctttgg ctaactcttt acgtatttct
    12661 gccagttttt ctgcagggat gccaatcccg ttgtctataa ctttgaattg gatcatctca
    12721 tcaattttgc atgtggtaat acgaataaat cctttgtgac ggacgtactt cgttccatgg
    12781 tatacagcat tttcaatcaa tggctgcaag atcattttta aaatagaaac aggaaaaagt
    12841 gtttctggta tatcgatctt atagtcaagc atatggccgt atcttatttt taagatgact
    12901 agataatcat tgatatggct aatttctttt tcaagaggaa tccagtcttg tcctttgctt
    12961 aaagacgtac gaaaaaagcc ggaaagtgca tgggtcatag tcagagcttc ttcaaagcag
    13021 tcttgttcga tcaaagaaat aatggcatcc aaactgttat aaataaaatg gggagtaatc
    13081 tgagcttgca atacacgaac ttcactttgt gctaaatggt attgtttcaa agcgttctcc
    13141 cgtaaagact ggtttagatc ttccgccatt ttattcatgc tagcggtaag tgtttgtaat
    13201 tccagtgttt ccggcagggt taaacgaaag ccaaggttcc ctgaagaaag ttgttctgac
    13261 atctcgatca gctgattgat cggttcttgg acctttctct ctaaaaagcg acgattcttt
    13321 ttcgcataga aaaaaatggt actaagaact ccaagttcca ctattgttaa tagaatgagc
    13381 gagcggacaa gtgtcgtatt ttttttgctt gctaagtcga tctccacacg aacaaaatct
    13441 tgtaagatat ctgacagcag ttgagtgaca gaatctacct gattgatgat ctcttcattg
    13501 gtatccataa aatcttttgc caagatatta ttgatcagct cgttttgata atcttccagt
    13561 gtatcgatcg tacgtagcga tacatctaac gtactaattt ctttaagtgt atttgtatta
    13621 tttttgatct ctcgaatatc attcctaagt tcttctagga tattttcctc attgaactca
    13681 tctacggtta cttggccaga aatcaagtcc cacatacttt ccaaaacttt ttgatcaacc
    13741 ttcaaagcaa ttgcatctgc tttttggata tttgcgatcg tctgttgata aatcaaaagc
    13801 tgttgcgaat ataacagtat gctcagtagc aaagggataa cagcaaatag aagcatggct
    13861 ttgtttgctt gggcaaaaat ctgttttatg cttaaatttt ttgtcatgat acttctccag
    13921 tcaataattt ctggttggga tgttagctat attctgctgg gaaaagacag tctcggaaat
    13981 aaaaatatct ttaggtaatg tttttcctgt ttgaatattt ccaaaataac gagtaacggt
    14041 attggctaca taccagccga catcaggttt acactcgact acacaattga cgattcctga
    14101 gcgtagcttc tcgatcatat cttcttgtcc atcaatagtt acaatgatca aatcttctcc
    14161 aggtttgatt gatgtttctt ttatcgcagc taaagcaccg tgagtcattt catcactatg
    14221 gctgtataat acatcgattt cggataaatc atttgtttct aaatatcgtt tcatctggtc
    14281 ttttcctttc atccgaataa aatcaccgac aattcggtga acaatagtca tcggaggttt
    14341 atggtcgatg gttccatctt tttttagggt tggtttacga ttgattgatt ccaaaaatcc
    14401 ttcggagcgt agcttggtcg gtgtggagtt ctctgaacca gcaagttcta ggattctgat
    14461 ttcctgtttc tttgaatttg tgaagtgatt gctgacaaat aaacccgccc gatttccttc
    14521 tgctttgaaa ctggggccaa tgtgggtgac gaaaagctcg ggatcattcg tttgaatatg
    14581 tctgtcgaca agtatgacag ggatccctgc tcgctttgct tcttttaata cagagtccca
    14641 cccggattct tgcaaaggag taaaaatgat catatccact ttataggcaa tgaacgtccg
    14701 tatatcttgg atttgccgtt cctgattcat gtagccattt cggtagagaa cttggtaatc
    14761 ttctttttca agcgcagtga taattgattc agtatgcttt ttccgccagc tactctccgt
    14821 tcctgattgc gaaaaaccaa cgactagtgg gtcaggtgct tcctttgtat taggtgtaca
    14881 ccccatcatt tggtaagcga ttacaacaag ggctaaaaaa aataaaacac gtttcacttt
    14941 aaatcactcc atctggattt gttagtacta tcttatcaga taaaagggaa aatcaaagtc
    15001 aaaaggaatc aatcgaaaaa aatccaagaa acttaaaaaa atccaagaca aaaaaagtac
    15061 acctaaaaaa tttaaagtaa atcccataaa aaaatcaatg ttaacgcttt ctttattagc
    15121 tatactcggt gtatcaattg aaatggggga taaaaaatga aaaacaaatt tagaaagatt
    15181 gttgggacta tcgctatttt gaccgcaagc attctattaa gtgcgtgtgg caattcagga
    15241 tctgcagatg atagtacggg gtatgtagga atcgctatgc cgaccaaatc agcagaaaga
    15301 tggatcgctg atgggaacaa catggtctcg gaattagaaa aactgggtta taaaacagat
    15361 cttcaatatg gagaagataa agtagaaaat caagtagcac aaatagaaaa tatgattaca
    15421 aaaggggtag atacacttgt gattgcttct atcgatggtt cggctttaac tgatgtgtta
    15481 gctaaagcaa aagaagcaga tatcaaggtg atcgcctatg atcgtttact gatgaattca
    15541 gaaaatgtcg attattacgc gacttttgat aattttggag taggtgtatc gcaagcagct
    15601 tatattgaag agcacttagg attgaaagaa ggaaaaggac cattcacgat tgaattattt
    15661 ggtggctcgc ctgacgacaa caacgcacta atcaattaca acggagtgat gtctgtttta
    15721 caaccatata tggacaacgg tcagttggtc gtgccatctg gacagacgag tttcagtcaa
    15781 atcgctacgc ttcgctggga tggttctacg gcgcaagcaa ggatggataa tctgttaagc
    15841 gcaaattata ctgaccaaac attggatgct gttttgtcgc catatgatcc aataagttta
    15901 ggaatcattt cgtcattaaa aggcgtgggg tatgggagtg aaagtaaacc tttacctgtg
    15961 attactggac aagatgcgac agttgctggc gttaaatcga tcattgccgg ggaacaaaca
    16021 caaacgatct ttaaagacac acgtatctta gcaaaaaata cgattgaaat gatcaaagca
    16081 atcagtgatg gagaagaggt tccggtcaat gatactgaaa cttatgataa tggggtgaaa
    16141 actgtcccta cgtatttagc aaatacagta tctgtcgata aagataatta tcaagcagag
    16201 ctgatcgaca ccgattatta taaagaatcc gatctgaaaa attaagagga accaaacaag
    16261 gaactaaaga gatctaagtc agagagagga taagaattat ggcggattgt attttaaaaa
    16321 tggcagatat caccaaaaaa ttttctggaa tccgagcgtt agattgcgtg aatctgatga
    16381 ttgaacgtgg ggaaatccat gcattgtgcg gagaaaacgg agcgggaaaa tcaacattga
    16441 tgaatgtctt gagcggcttg tatccatatg gctcctatga cggggaaatc ttttatgatg
    16501 gaaagctgtg tcaatttaaa gatttgaaag atagtgaaga aaaaggcatc gtcatcattc
    16561 atcaagaatt agcactaagt ccttatcttt caataaaaga gaatattttt ctcggaaatg
    16621 aacaagcaga aagaggcgtg attgattggg aactgacaga aaagaaaaca tatgaactgc
    16681 tgaataaagt cggtttgtct atcgacccga atcttctagt atcccagatt ggcgtagggc
    16741 aacagcaact agttgaaata gctaaagcat tatcaaaatc cgtccgtctg ttgattttgg
    16801 atgagcctac cgctgcattg aatgaagaag aaagcgcgaa tttgctgaga ttgatccgag
    16861 agttgaaaga gcaaggtgta acctccatca ttatttcaca taagctgaat gaaatcgtgg
    16921 atatcgcaga tcgaatcacc attttacggg atggacagac gattgagaca ctagaaaatg
    16981 aaatgatcag cgaagagcat attattcgcg gaatggtggg cagagattta gttgatcgtt
    17041 atcctgaaag aaaaccggat attggagaag tttattttaa agtagaagac tggacggtct
    17101 atcaccctct tgatcacgag cgaatcataa acgaccacat caacttgcat ttgaaaaaag
    17161 gagagattgt aggaatagct ggactgatgg gagcgggcag aactgaattc gctatgagtc
    17221 tatttggaca ttcttatgga agcaagatat ccggacgtat attaaaggaa ggggcagaga
    17281 tatcagttaa gaccgttcct tcagccattg caaacggact cgcttatgtc tcggaggacc
    17341 gaaaatcttt aggactgaat ctgctgatgg atattcggga aaacgtgacg ctgagcagtt
    17401 tggataaaat aagtcaaaaa ggagtattag ataaagaaaa agaagtaact gaggcagaaa
    17461 atttccggaa aaagatgcgt ataaaaacaa actccatcca tcaaattgtc agtagtctta
    17521 gtggtgggaa tcaacaaaaa gtcgttttat ccaagtggct gatgacacaa cccgatattc
    17581 tttttttgga tgagccaacc cgtgggatag atgttggggc aaaatatgag atctacacga
    17641 ttatcgaaga aatggcagca aatggcaaag ctgtctgtct tatctcttct gaacttcctg
    17701 aaatattagg gatgtgtgat cgaatctata cgatgaacga aggacgtatt acaggagaag
    17761 tctcgcgaga agatgcagat caggaagtct tgatgaaatt aatgacgaaa gaagaggtgg
    17821 gttaagttgg aacaggcaaa aaatcttacg gataaaaaag agaaaaaact aaatttgaaa
    17881 agtagagcag ctgatctttt tggtcgctac agcatggtga tcattttagc gggactactt
    17941 attgcgtttc aattgatgac ggatggtatt ttctggcgtc cattgaatat aactaacctt
    18001 gttctgcaaa atagccatat attagtacta gctgcaggga tgttattagt tgtgctctta
    18061 ggtcatgtgg atttgtcggt agggtcagtg atggcgtttg ttggtgcaat tgcgggcatg
    18121 atgatggtca atcgccaaat gaacccgtgg atgacagtta tcgcatgtct tgcagtaggc
    18181 gggctgatcg gcgcatggca aggtttttgg gtagcttatg cagggatacc ggcgtttatc
    18241 gtgactttag caggattgct gatgtttcgt ggactgacac aagttgttct aggaggccaa
    18301 tctctagcac cttttccagc tgttttccaa aaaatgtcta cgggttattt accagactta
    18361 agcgaaggaa gcagccttca cttcttgaca ttgattttag gaatgatcat cacgatcgtg
    18421 ttagttgttg gtcaatggcg aacaagaaat cgtcggaaaa atcatttatt tgaagtggaa
    18481 tctccaacag ctttcttggt aaaggcggta tttacagcag tcgttgtact aggaataact
    18541 tatatctttg cctcttatca agggtatccg attattttga tcattcttgg tttgatcgtt
    18601 gcagcatatg ggtttttgac aaataaaaca gtagcaggtc gacagattta tgcgacgggc
    18661 ggaaatcgaa aagcggctga actatcaggg atcaaaacaa agcgaatcac gttctgggta
    18721 ttcgtcaaca tgggagtcat ggcagcttta gctggattag ttttagctgc tcgattgaat
    18781 gcggcaacac ctcaagcagg tacatcgatg gaactggatg caatggcggc agtatatttt
    18841 ggtggtgctt cgacttccgg cggcattgga acaatcatgg gtgcgattgt cggtggcttg
    18901 gtcatgggtg tattgaacaa tggcatgtcg attttaggcg tcggggtaga ctggcagcag
    18961 gcaatcaaag gattgatctt gcttttagcc gttgtgctag acttgtataa taagaaaaaa
    19021 cgaacaacgt aggagaacgc cggttattca gaaaaaagac tgtgacaaga catttgtcac
    19081 agtctttttt tcgctaaaag cgaacattca ttgtttcgtt tgtatagact aaaaataata
    19141 tatgtttttt taccattcgt ataaattctc tttttgctct tgattttttt gcttaattga
    19201 gttaaattac atagggcgct aaaaaaagcc ttgattaaat taataatgtt cgtgttttgc
    19261 tggatgtgaa catcaggagg ataatgtaat gaaagtattt gattacgaag atattcaatt
    19321 aattcctaat aaatgtatcg taaatagccg ttctgaatgt gatacaacgg tcacattagg
    19381 taaacataca ttcaaaatgc cagtagtgcc tgccaacatg cagacgatta ttgatgaaac
    19441 aatcgcagaa ttcttagcgg aaaatggtta tttctatatc atgcatcggt ttgatgaagc
    19501 agctcgaatt ccgtttatca aaaaaatgaa aaagcgtgga ttgattacct cgatcagtgt
    19561 tggcgtcaag aaagaggaat attcctttat agagaagttg gcagaagaat cgctaaatcc
    19621 tgattatatc actatcgaca tcgcgcatgg acatgctaac tcagttattg atatgattca
    19681 gcatattaaa aaatatttgc ctgaaacttt tgtgattgcc ggtaatgttg gtacaccgga
    19741 agccgtccgt gaattggaaa atgcgggcgc agatgcaaca aaagtcggga ttggtccagg
    19801 aaaagtatgt attacaaaaa tcaagacagg ctttgggact ggaggctggc aactggcagc
    19861 tcttagatgg tgtgcgaaag cagctcgcaa gccgatcatc gctgacggtg ggatcagaac
    19921 acatggcgat attgcaaaat ctgtccgttt tggtgcgaca atggtcatga ttggttcatt
    19981 gtttgccggt cacgaggaat ctcctggcga aacaaaagtc gaaaatggaa tcgtgtataa
    20041 agaatacttt ggttctgctt cagaattcca aaaaggcgaa aaacgaaatg ttgaaggaaa
    20101 gaaaatctgg atccaacaca aaggatcttt gaaagataca ctagtggaaa tgcaacaaga
    20161 ccttcaatca tctatttctt atgctggagg aagagatctg gaagccattc gaaaagttga
    20221 ctatgtcatc gtgaaaaatt cgatttttaa tggagacaca atctaaaaaa taaataaaat
    20281 attaggatgt tgtgaaaaag t
//
LOCUS       DAMEET010000053        20285 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.054, whole genome shotgun sequence.
ACCESSION   DAMEET010000053 DAMEET010000000
VERSION     DAMEET010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.054"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            140..460
                     /locus_tag="PB780_001684"
     CDS             140..460
                     /locus_tag="PB780_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733738.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="PB780_001685"
     CDS             912..1112
                     /locus_tag="PB780_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDE0733739.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="PB780_001686"
     CDS             1259..4048
                     /locus_tag="PB780_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDE0733740.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="PB780_001687"
     CDS             4095..4622
                     /locus_tag="PB780_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HDE0733741.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="PB780_001688"
     CDS             4643..5833
                     /locus_tag="PB780_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDE0733742.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="PB780_001689"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="PB780_001689"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDE0733743.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            8224..8898
                     /locus_tag="PB780_001690"
     CDS             8224..8898
                     /locus_tag="PB780_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDE0733744.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            8895..9236
                     /locus_tag="PB780_001691"
     CDS             8895..9236
                     /locus_tag="PB780_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733745.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            9266..9625
                     /locus_tag="PB780_001692"
     CDS             9266..9625
                     /locus_tag="PB780_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HDE0733746.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            10149..12230
                     /gene="cadA"
                     /locus_tag="PB780_001693"
     CDS             10149..12230
                     /gene="cadA"
                     /locus_tag="PB780_001693"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDE0733747.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            12541..14007
                     /locus_tag="PB780_001694"
     CDS             12541..14007
                     /locus_tag="PB780_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDE0733748.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            14622..15161
                     /locus_tag="PB780_001695"
     CDS             14622..15161
                     /locus_tag="PB780_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HDE0733749.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            15235..15675
                     /locus_tag="PB780_001696"
     CDS             15235..15675
                     /locus_tag="PB780_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HDE0733750.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            15688..15897
                     /gene="copZ"
                     /locus_tag="PB780_001697"
     CDS             15688..15897
                     /gene="copZ"
                     /locus_tag="PB780_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HDE0733751.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            15897..18083
                     /locus_tag="PB780_001698"
     CDS             15897..18083
                     /locus_tag="PB780_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDE0733752.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            18103..20274
                     /gene="copB"
                     /locus_tag="PB780_001699"
     CDS             18103..20274
                     /gene="copB"
                     /locus_tag="PB780_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HDE0733753.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
BASE COUNT         6777 a         3527 c         4407 g         5574 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaacggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactg caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ctatcatcat
     5341 caacggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtgggaaa
     5401 tcctgtgatc atcaatggaa tgatcgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggcgag caagatacag tggaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatattcg cttgtggcag aactgccggg
     5581 tgtttctctt gaaaagccac aaggagcttt ttatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctaaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcgc atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatat gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttatgatga
     6721 tgcgatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtgat
     6901 ctgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacagactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggtgatg aaaaagctgt tctgcatcaa
     7201 gtaattagaa cagccaagca aaaacagctg ttcatgtttt tgaattggcg ggacttatta
     7261 ccgcagatgt aagcttttga ataccgttta gtggagaggt tgtgaaaaag ctgtttagtt
     7321 tcgagtatta agaaaaattt tgtggaaatg gctttccaca tttttgcata aatttggctt
     7381 aataccgaga aactggcttt tgaacaccgt ttaaggaaga ggttgtgcgc ctgccgttca
     7441 gctccgagtc acaaggagta atttcaaaaa acagtttttc atgtttgatg actattgtga
     7501 cttgtgaccg aggagttggc cggagagcac cgtttattct aaaaaagaac tgcgaactct
     7561 cgcagttctt tttttaaaat acgggataaa ggaagtttcc agcagacaag agaagaaagc
     7621 aaaagaaatt gctatttgat tcacaaggtt ctataacgaa atcgcaatca atcaaacatt
     7681 tacaaagaag gtaattcaaa tggaaatctg atttgatgat gcttctgctg ttgaaataaa
     7741 gcagcatcta gcacctgaaa acaaactaat agtaacattc gaagatggag taggtccgta
     7801 ttttcagcat gcaataatcc atatgtaagt tcaattcacc cttgaaatca tcggaccaga
     7861 taaagttatg accaatctat cccaagcaat ctagaagaga tattagtaaa gggacattta
     7921 ctagaagtct tagatgagcc tataaatata tgttctgatg gcgtcatcag tggtctgatt
     7981 gacggaagct taggtgtgat cgatctctct ggaccaaaca acgaaggaat caaggggaat
     8041 ccggcacgtt aagaaaaaaa tagcataaaa aactagacac ttgatgagcg ttcattcaat
     8101 tgtttgaagg gactaggctg gtgtcatttt ttatacatat attttctctt tcgtcttgat
     8161 agggcttatg ggataatata aggaagaaaa tcatcgtttt attcaaaaag gagtttttga
     8221 caaatgaaaa aacgtatatt atttatcggt gatagcatta cagacgcagg aagagaccga
     8281 gcagaagcaa ctgatctagg taaaggttat ccacttttga ttgccggaaa actaatctcc
     8341 caatatccaa cgatggaatt tgaatgcatg aaccgtggga taggaggaga caaaattgca
     8401 gatctgcaag tacgctggca agaagattgt ttagctttac aacctgacta tgtatcgatt
     8461 ttgatcggaa tcaatgatac ctggcataat actagcgatc aggctatatt cggaactgag
     8521 aaatctgctc agcaatttga atccgtttat cgaaaattac taaatgaact tcaaacagtg
     8581 ggaatctgca acatcatctt gatggagccg tttgtacttc ctataaccga cgaccgtcat
     8641 ggctggcgta aagacttaga ccctaaaatc caaattgttc gtaaacttgc taaggaatat
     8701 ggcgcattgc tggttccttt agatggtata ttcaatgctt tagggattgt ttatggtttt
     8761 tcgaaatata caagagaaga tggggtccac ccaacgattg gcggacatga gatcattgca
     8821 caaagctgga tagatgcaat tgccgcagtc gattttttta gtggagataa aaatgagcga
     8881 aagaaaagtg agaaatgatg gaaatacggt gggcagtaaa agaagatcta cctcaaatca
     8941 atcagctgat tactgatcat ccagaatcat tcgtaaaagc aaaaacggca gctgacctaa
     9001 atgataagaa taaactattg ctcgttgctg taacaaaaga aaaacggatt atcggatttg
     9061 cgcaagcgga cgtgcaacag tcagaagaac gattggatca gcgaccatca ggaaaactaa
     9121 tccaacacac attagtaaca aaagaaaata cggaagaaat ccaaaaaaaa tttgcagaat
     9181 atttgatact ggaagctaaa aaaagaagaa taaaatcatt ttttgcccag ttttaatcaa
     9241 aaacagtaat agaaggagaa atacaatgag ttgtttagga aatgtcatct ggtttatatt
     9301 cggcggacta atcggtggtc ttagctggtt agcagcgggg atactttggt gtatcacgat
     9361 cataggtatc ccaatcggta tgcaatgttt taagatcgcc tccctttctt ttgcgccttt
     9421 tggcaaagaa gtaatttatc accagtctgg catgtcattg attgttaata tcatctggct
     9481 gatcgtttca ggcctaccaa ttgctctcgc acatgtcgtc agcggcatcc ttctatgtat
     9541 cacgatcata gggatacctt ttgccaagca atctttcaaa cttgctcgat tggcgttgat
     9601 gccattcggt gcagaaattg tataaaagag aataataaag gttagataaa gcatagagtc
     9661 aggtgggata catgtatttc acctgactct atgcgttctc ctgtataagt tcttccatgt
     9721 gacttttacg tcttttattt atatggggtt actgtttatg gaaagttgaa ataattttca
     9781 ttaaagaact ctttctatat ttttgaaagc gtagtggagc ttcgaatatc tgggaaggac
     9841 attttgcagg gattgaaaaa agatattgtc ctatcgaaaa attaatgaat ttagacaaat
     9901 tgccagcaac aggcttcaaa cttagttgct atcctgtcaa gatcaagaag gcaagtgcgg
     9961 gttggataag agcagtggca atgatcgaaa agaaaaagaa agaataagcc aaaacatctg
    10021 tatggaagaa attttccgat gcagatgttt ttcatttttt ctttcggata ccttgacata
    10081 tgaataaccg ttcatttata attagttatg aagagatatt catatgttga ggcgaggagg
    10141 tcattaaaat gaaaagttac agattagaag gtttagattg tgcgaactgt gcaatgaaaa
    10201 tcgaaaaagg agttcagaag atcaatggag tcaaggaagc aacggtaaac ttcacttctg
    10261 gaaaactaac gattgacgca gaggaagatc atttggctac gattgaacaa gaaataaaaa
    10321 aagtagtgaa ggaattagaa ccagatgtca aagtaacaga aatagataag gaaaaagtat
    10381 ctgaacatgg aaatgaaaaa gagagaaata cgctgttccg gattttattt tcccttgcag
    10441 gtatcgcttt attgctatta tttgatttta atgaaccaat tcgcttgata gggtatttat
    10501 tgatttatct gctgatagga tatgatgtag tgaagaaggc agtgatgaat atcgtcaaag
    10561 gaaaaatctt tgatgagaat tttcttatgt ctgttgctac gattggcgct atgttgatcg
    10621 gtgaatatcc agaagccgta gcagtgatgc tattttatca aatcggcgag tatttccaag
    10681 gattggcagt cagtcattca agaaaatcga ttcgcgaatt gatggcgatc cgtccagaaa
    10741 cagctcacgt gcagacgacg gaaggattga tgaccgtgaa ccctgaagac gtgcagatcg
    10801 gtcagtttgt acttgtgaag cctggtgaac gtgtaccgtt agatggaacg atcatcgaag
    10861 gagaatcatt agtcgatacg tcagcactta caggagaatc agtccctaaa tctgtatatg
    10921 ttggtgaaac agtattaagt ggatttatca ataaaaataa accattttta gttcaagtag
    10981 aaaaatctta tgaaaattct actatcagca aattattaga attagtagaa aatgccagca
    11041 gtaaaaaagc accagctgaa aatttcatta ctaaattcgc gcgctattac acaccagtcg
    11101 ttgtaggatt ggccgtttta ttagctgttt tacctccact tgttgtttct ggagcggcgt
    11161 tttctgaatg gatctatcga gcattgacat ttttagtgat ctcatgtcca tgtgcattag
    11221 tgatctccgt accccttagt ttctttggcg gtatcggagg agcaagtaaa atcggtgtat
    11281 tagtaaaagg ttcaaattac ttggagttgc ttgcacaaac tgagacagtg gtattcgata
    11341 aaaccggcac attaacgaaa ggtgactttt cgatccagac gatcgataca aaaatcgatc
    11401 caaagatatt catgcaatat gttgcgagtg cagaacagtt ttctacacat ccgattgctc
    11461 aatctgtttt agaaggctac gcaggtccgc ttttaccaac agctaatatc caagaatttg
    11521 caggtgaagg gatcttagct gaagttgatg gcaaacaagt attagcaggt aatcacaaat
    11581 tgatggaaag atttgaaatc tcttttcctt caagtcaaga aatcggtaca ttgctttatc
    11641 tagcgatcga tcaaagttac agtggttatc ttgttattgc agataccttg aaagaagatg
    11701 ccgtagacgc attggttcaa ttaaaaaagg ctggtgtgaa gaacacagtc atgttgacgg
    11761 gcgattcgaa aaaaattgct gatcatatcg ggaagcaagt aggcgtggac aagatatata
    11821 gcgaattgct tccagaagat aaagtgcagc ggttagagga aatcttacaa agaaacaata
    11881 aaaagaccgc atttgtcgga gatggaatca acgatgcacc ggtgctagca agagcagatg
    11941 taggaatcgc aatgggtgga ttgggaagcg atgctgcaat tgaagcagca gatgtagtca
    12001 tcatgaatga tcaaccatcg aagattgcag aagcaatcca tttagcaaag aaaacattga
    12061 aaatcgtcaa acagaacatt gtctttgcta ttgggataaa aattttggta cttgccttag
    12121 gtgcttttgg ttttgcatca atgggagatg cagtatttgc agatgttggg gtgacagtat
    12181 tagctgtttt gaatgccatg cgaagcttgc atgtaaaaaa taaacgttga tcaatgaaga
    12241 ggacgaatga acgtcctctt ttttttatat ccttttctta atgaaaaatg taaagaaaat
    12301 agataggatt ataaaataac taatgttttt aattttgcag aaaattcaaa atagcttgaa
    12361 attcaataag gtaaatgaaa taaaagaaaa taacttattt ttttgatgga aaaaatatga
    12421 aaaaacgatg tgtattaaat aaaagaatac acacataaaa taacactgtc ttgtaagttt
    12481 gttaaaaatt gaatatgatt acatctggat aacaaatttt tatcataaag gagagaacga
    12541 atgaacaaca aacaattacg ctggtttact gttgcgttaa ttgcatttaa catggtttgg
    12601 ggcatgggga atgtagttaa taactatgct cagcaaggag tatcagttgt cacctcttgg
    12661 ctattgattt tggccattta ttttattcca tacgcattga tcgttggaca attagggtca
    12721 gccttcaagg atagtaaagg aggcgtcagt tcatgggtcg aaaatacgac atctaataaa
    12781 cgactggcat actatgccgc ttggacttac tgggtcgtcc atattcctta tttggcacaa
    12841 aagcctcaag caatcctgat tgctggcggt tgggcagtcc aaggaaatgg aaatatcgta
    12901 aatacgatgt cagtacaaat ggtagcagtt atttcactta ttatatttct tgccttttta
    12961 tatttatcaa caaaaggact ttctactttg aaagtgattg gcggactggc cggaacagcg
    13021 atgttcgtca tgtcgatctt atttattctt ttagctgtaa cggcaccgtt catcaatcca
    13081 acgatggaat ttgccacacc acatatgaat caagtgaaaa catatattcc aacatttgat
    13141 ttcagttatt tcacaaccgt ttcgatgcta gtatttgcag ttggaggagc ggaaaaaata
    13201 tccccttatg tcaactctac aagaaatcct gcaaaggaat ttcctaaagg gatgatcttc
    13261 ttagcagcaa tggtagggat ttgtgctgta ttaggctcag tcgcaatggg tatgctgttt
    13321 gctagcgata atattcctaa agacttgatg gcaaatgggg catacagtgc ttttcaaatt
    13381 ttaggtaatt actatggtat cggtaactca ttgatgatca tttacgcatt gacaaacatg
    13441 attggacaaa tctccgcatt agccttttca attgatgcac cactaaaaat cttgttagca
    13501 gatgcggatc cagaatttgt accaaactgg ctacgtaaac gtaatcgtaa aggtacattg
    13561 gtgaatggtt atcttctgac aggtatacta gtaggaatca ttattttgtt gccgatcttt
    13621 ggtatcaaag atatgaatga attagtaaaa tggttgacga acttgaactc agttgtcatg
    13681 ccaatgcgtt acctatgggt gttcttggca tatatgatgt tgaataaagc ttataagaaa
    13741 ttcaattctg aatataaatt tgttaaaaat ccaaaaatcg gttttatctt tggtgcttgg
    13801 tgctttgcct tcactgcgtt tgcatgtatc ctaggaatgg tgccaaaagt agattatact
    13861 gctgatccta aagcatggtg gttccaatta ttcacgaata tcatcacacc aattgtcttg
    13921 atcttattag gtatgatctt gccagcaatt gctcgtcgag acaaaaacaa agaaatcact
    13981 gaaccagaaa cagtggttca gccttaaaat agaaatactt gttacaagag gttgtgacag
    14041 aagtggtcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
    14101 aagtttcggc ttgtttttcg cagaagctac ttctggaaca ccgtgaatca gaggttgtga
    14161 aaaagctgtc ctgcatcaag taataagaac agccaaacaa aaatagcccc tcatattttt
    14221 cgttttgctg ggcttattac cgcagatgca agcttttgaa caccgtttaa gaaagaggta
    14281 gtgagcctgc cgtttaactc cgagtcacaa ggagcaattt caaaaaacaa cttctcatgt
    14341 ttgttgacta ttgtgccttg tgaccgaggt gttggcagga gaacaccgtt taaggaagag
    14401 gttgtaaaat cagcgatttg acctgagtat tagaggaaca aacgaaaaaa tagctcctca
    14461 tattttgccg ttttttgagt taatgctgaa ggtcagctga ttgaacacca tttattcgaa
    14521 acaagggact ttgacacgac ttttgtcaca gtcccttgtt tttttgcggc gttataagtg
    14581 gtatgatcga accaaattcc aaagagaagt gagggagaag aatgaagatc atcgtgattg
    14641 gacaaagtgg gtcgggaaaa tcgaccctcg caaggaagat caaagaaatc accgactttc
    14701 cgctactgcc gctagacctt ttatggcata caacagacta cagtattcag gcaaaaaagt
    14761 ggtttttgca agaacagcaa tcgtttatgg tatctaatcc gtcatggatt gttgaaggaa
    14821 attatacttc tacattagag gaaaggatta aagaagcaga taagatcatt tggcttaaag
    14881 tgccgcgtta tctcgccatc taccgagtag tttggcgttc tctaaaaaga aagataaaca
    14941 aaaaatctcg gccagatatg cctgaatcat tttcagagaa actaaacagg gaatacttag
    15001 aatttttatc ttttatctgg cattttgaag aaaacaatga accgaaaata cagcagctga
    15061 tccaagtagc taatgcaaga aataaactga tcatccttaa aacaagacga gagaaacaag
    15121 aatttttaac taaattgaaa atagagagca aaaataattg acagtttttg tttacaagcg
    15181 taatctgtat tcataagcta atgattacat ttgtaaacga aaggtgtgaa ataaatggaa
    15241 aaagtcaaaa cgccagtaaa gatcagtgat tcagagtggg agatcatgcg tgttatttgg
    15301 acacttggac agacgacagc ccaagaaatc acgcagatct tagctgattc gatggattgg
    15361 aaaccagcaa cgatcaaaac tttgctggga cgtttggtaa aaaaagaagt gatctggaca
    15421 gaacaagaag gaaaaaaatt catttatcat ccagctgtct cggagatgga aacagttcgc
    15481 agtgcgacgg aaaatctttt ttcccatatc tgtgcaaaaa ggattggaga gacgattgcg
    15541 gatctagtag aagaagccac tttgactcaa gaagatatca atgagatttt gaaacaactg
    15601 gaacaaaagc aaccagtaga tacgatcgaa tgtaactgta tcccaggtca atgtgaatgt
    15661 aaacaacatc aataggagga agaaaaaatg aaacaacaat tttcaatcga aggtatgagc
    15721 tgcaatcatt gtgcagcaag agtagaagaa acagtaagcg cacttgacgg cgtgcaaaaa
    15781 gtgaaagtaa atttgaaaaa agcaaacggt acggtcaagt ttgatgaaac aaaagttcaa
    15841 tccgaaaaga tttgtcaagc aatcaatgga ttgggttata aagcagaggt gatttgatgg
    15901 caacagcaga aaccaaaatg gatacgttcg tcattacagg tatgacttgt gcaaattgtt
    15961 ctgctaggat cgaaaaagag ctgaacggac aacctggtgt ggtcaatgcc acagtcaact
    16021 tggccactga aaaagcaagt gtcaaatatg aagggacgac cactgaaaaa ttaatccaaa
    16081 gcgtagaaaa catcggttat ggtgcaattt tgtacgatga agcccacaaa caaaaaattg
    16141 cagaagaaaa gcaagcgtat ttgaaaaaaa tgctattcga tcttatattg agtacggttt
    16201 tgactttgcc gttgatgttg tcaatgattg ctatgatgtt aggaagccat gcggcgattg
    16261 ttcacttttt tcattttcca atcgtccagt tagtgctgtc tgctcctgtc caattttatg
    16321 tcggtgctcg tttttataaa ggtgcgtacc atgcaatcaa gacgaaagca ccaaatatgg
    16381 atgtattggt agcaattgga acatcggctg cctttgcatt gagcatttac aacggatttt
    16441 tccgtggaca cccccaagat ctttattttg aaagcagcag tatgatcatc acgctgattt
    16501 tgctcggcaa atatttggaa catacagcaa aaacgaaaac aggtaatgca atcaaacaat
    16561 tgatgtctct tcaaacaaaa accgcccaag tgataagaaa cgggaaagaa gaaacgttag
    16621 cgattgaaga agtagtcgtt ggtgatcaac tagtcatccg tcctggtgag cagattccag
    16681 cagatggtcg aattatttca ggaagcagtg cgatagatga aagtatgctt actggtgaaa
    16741 atcttcctgt agaaaagaat ccggatgaca cgctttttgg cgggacaatc aatacgaatg
    16801 gtctgctaca catggaagtc acacaagtcg gcaagcagac agtattagcg caaatcattc
    16861 aaatggtgga agatgctcaa gggagcaaag ccccaatcca aaaaatcgca gatcggatct
    16921 cagggatttt tgttccgatc gtattagtca tcgcgtttat cacgctcata gctacaggtt
    16981 tgatcactgg tgattggcaa ctcgcattga tccatagtgt ctctgtatta gtcattgcgt
    17041 gtccatgtgc attaggttta gcgaccccaa cagctattat ggtaggaacg ggtgttggtg
    17101 cacgaaatgg aattttaatt aaaggcggag aagcattaga agcagctgct cacttagaca
    17161 gcattgtttt ggataaaact ggaacgatta cagaaggaaa gcccaaagtt acagatctag
    17221 tcggttcaaa agaagtccta tctatttttt atactttaga acaagcttct gaacatccac
    17281 taggtaaagc aatcgtggaa tacggaaaac tgcaagaagc agcaacatac gatatgattg
    17341 attttacagc ccacccaggt gctgggatca gcggtacgat taatggagta cgctattttg
    17401 cgggtacacg taaacgatta attgaattga acctatcatt tgatgaatac caagaacacg
    17461 cgttagaatt agaacagcag ggaaaaacag tcatgttttt agctgatgaa aagcaagtga
    17521 tcgggtggat tgccgtggct gatcaaatca aactagaagc caaacaagca atcaagcagc
    17581 tccagaataa aggattagat gtttttatgc tgacaggaga caacaaactg gcagccgaaa
    17641 cgattggtaa acaagttggt attgatccaa agcatatctt tgctgaagta cttccagagg
    17701 ataaagcagc ctacgtagaa aaacttcaga aagatggcaa aaaagtcggt atggcaggcg
    17761 atggaatcaa tgatgcaccg gccttagcat tagctgatgt cggtatggca atgggaagtg
    17821 gaacggacat tgcgatggaa acagccgatg tgacgctgat gaatagcagt ttggcatcca
    17881 ttgcacaaac aatcgagttg tctaggttga cattgagaaa aatcaaacaa aatcttttct
    17941 gggcttttgt ctataatacg attggtattc cttttgccgc actaggcttt ttgaatccaa
    18001 tcattgcagg aggagcgatg gcgtttagtt ccgtcagtgt attgttgaac tccctgagct
    18061 taaatcgaca catgaaaaag taagagaagg gatggagtaa aaatgaatgg gaacgatatg
    18121 gagaaaaaac atgagcataa aaaagaagta gtaaacaaag aaagtacaga atctcatgaa
    18181 aaacagatgg agatgaaaca tgaccatagt cagatggatc attccatgca catgggacat
    18241 aaccatggag atatagcaaa cagcaaacaa atggaccatt cgatgcacat ggatcacgaa
    18301 cacggcggca tggatcattc catgcacatg gggaatttca aacaaaaatt ttggctatcg
    18361 cttatcctag ctatcccaat cattcttttt tcaccaatga tgggaatgga atttccattt
    18421 caagtaacgt ttcctggttc tgattggctc gtattgatct tagcaactat actatttatc
    18481 tatggcggtc agccattttt aagtggagct aagatggaat tgaaacaaaa aagtcctgct
    18541 atgatgacac tgatcgcgat ggggattaca gttgcatata tttatagtgt ctattcgttt
    18601 atcgcaaatc tgctcaatcc acatacacac gtcatggatt tcttctggga attggctact
    18661 ttgatcgtta ttatgctact aggtcactgg attgaaatga atgctgtgtc gaatgccagc
    18721 aatgctttac aaaaactagc agaattactg ccagaatcag tgaaacgctt gacgaaagat
    18781 ggaaaagaag agacagtttc attgaaagaa gtaaacgaag gtgatcgttt gatcgtccgg
    18841 tctggtgata aaatgccaac agatggtgta atcttgaaag gtgaaacaat tgttgacgaa
    18901 tcagctgtga ctggcgaatc aagaggaatc aaaaaacaag ccaatgacaa agtgattggc
    18961 ggttctatca atggggatgg aacgatcgaa atcgaagtaa ctggaactgg tgaaaatggt
    19021 tatctagcga aggttatgga aatggtgcga aaagcacaag gagaaaaatc caagctagag
    19081 tcattatctg acaaagtggc aaaatggctg ttttatgtcg cattaattgt gggtatcctg
    19141 gcattcatcg cctggttgtt cttgacagat cttcctaatg cattagaacg tatggtaaca
    19201 gtctttatca tcgcctgtcc tcatgctcta ggtttagcta tcccattagt tgttgctcgc
    19261 tccacatcaa tagcagctaa aaacggatta cttttgaaaa accgcaatgc actggagcaa
    19321 gccaatgatc tagatgtgat catgctggac aaaacaggaa cactaacaga aggaaaattt
    19381 actgtaacgg gtgtcgaagt acttgatgat gcgttcaata aaaatgaaat ccttcaatat
    19441 ctaggagcct tggaagcgaa tgccaatcat ccattggctg ttggcattat gaattatttg
    19501 aaagaacatg aaatcaagcc ttatcaagct gaaaatctaa aaaatctttc aggagtcggg
    19561 ctagaggcaa cagtcaaaaa acaacaagtg aaaatagtta atgaaaaaga agttgagcgt
    19621 ctcggattaa acgtagaaca agcattattg aaaccttacc aagaacaagg aaataccatc
    19681 agcttcctga ttttagaaaa tcatctagct gcaatcgttg ctttaggtga cgtagtaaaa
    19741 acagaagcaa aagaatttat ccgaacattg aaagaaagaa aaatcactcc tgtcatgctt
    19801 actggtgaca ataaaaacgc tgcccaagca gtagcagatt atttagggat cgaagaatat
    19861 tatggcggtt tattaccaga tgataaagaa gctgttgtcc agaaatacct tgaccaaggg
    19921 aaaaaagtaa tcatggtggg tgatgggatc aatgatgcgc caagtttagc ccgtgcttca
    19981 atcgggatgg caatcggtgc agggacagat atcgcaatcg attctgcgga tgtcgtattg
    20041 acaaacagcg atccaaaaga tattttgcat tttcttgatc tagccaagca aacgcgaaag
    20101 aaaatgatcc agaacctctg gtggggggca ggttacaata ttcttgcaat tccgctagca
    20161 gccggaattc ttgcgccaat tggtatcata ttaagtccag cagttggtgc tgtcttgatg
    20221 tcgttaagta cggtagttgt agctctaaat gctttgacat tgaaaatcaa ataataatga
    20281 aaaaa
//
LOCUS       DAMEET010000054        19402 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.055, whole genome shotgun sequence.
ACCESSION   DAMEET010000054 DAMEET010000000
VERSION     DAMEET010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.055"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            4..1194
                     /locus_tag="PB780_001700"
     CDS             4..1194
                     /locus_tag="PB780_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDE0733754.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            1298..2053
                     /locus_tag="PB780_001701"
     CDS             1298..2053
                     /locus_tag="PB780_001701"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDE0733755.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            2169..3467
                     /gene="eno"
                     /locus_tag="PB780_001702"
     CDS             2169..3467
                     /gene="eno"
                     /locus_tag="PB780_001702"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDE0733756.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            3866..5005
                     /locus_tag="PB780_001703"
     CDS             3866..5005
                     /locus_tag="PB780_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HDE0733757.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(5072..5647)
                     /locus_tag="PB780_001704"
     CDS             complement(5072..5647)
                     /locus_tag="PB780_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDE0733758.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(5652..6626)
                     /locus_tag="PB780_001705"
     CDS             complement(5652..6626)
                     /locus_tag="PB780_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleosidase"
                     /protein_id="HDE0733759.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(6650..6694)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            6909..7877
                     /locus_tag="PB780_001706"
     CDS             6909..7877
                     /locus_tag="PB780_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDE0733760.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            7974..8267
                     /locus_tag="PB780_001707"
     CDS             7974..8267
                     /locus_tag="PB780_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDE0733761.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            8385..8621
                     /gene="secG"
                     /locus_tag="PB780_001708"
     CDS             8385..8621
                     /gene="secG"
                     /locus_tag="PB780_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDE0733762.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            8724..9479
                     /locus_tag="PB780_001709"
     CDS             8724..9479
                     /locus_tag="PB780_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDE0733763.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            9482..11839
                     /gene="rnr"
                     /locus_tag="PB780_001710"
     CDS             9482..11839
                     /gene="rnr"
                     /locus_tag="PB780_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDE0733764.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            11861..12325
                     /gene="smpB"
                     /locus_tag="PB780_001711"
     CDS             11861..12325
                     /gene="smpB"
                     /locus_tag="PB780_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDE0733765.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            12627..13346
                     /gene="atpB"
                     /locus_tag="PB780_001712"
     CDS             12627..13346
                     /gene="atpB"
                     /locus_tag="PB780_001712"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDE0733766.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            13399..13614
                     /gene="atpE"
                     /locus_tag="PB780_001713"
     CDS             13399..13614
                     /gene="atpE"
                     /locus_tag="PB780_001713"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HDE0733767.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            13705..14229
                     /gene="atpF"
                     /locus_tag="PB780_001714"
     CDS             13705..14229
                     /gene="atpF"
                     /locus_tag="PB780_001714"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDE0733768.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            14216..14758
                     /locus_tag="PB780_001715"
     CDS             14216..14758
                     /locus_tag="PB780_001715"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDE0733769.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            14781..16337
                     /locus_tag="PB780_001716"
     CDS             14781..16337
                     /locus_tag="PB780_001716"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDE0733770.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            16351..17253
                     /locus_tag="PB780_001717"
     CDS             16351..17253
                     /locus_tag="PB780_001717"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDE0733771.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            17276..18682
                     /gene="atpD"
                     /locus_tag="PB780_001718"
     CDS             17276..18682
                     /gene="atpD"
                     /locus_tag="PB780_001718"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDE0733772.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            18696..19118
                     /locus_tag="PB780_001719"
     CDS             18696..19118
                     /locus_tag="PB780_001719"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDE0733773.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         6505 a         3517 c         4153 g         5227 t
ORIGIN      
        1 aaaatggcta agaaaacagt gaaagatatc gatttaaaag acaaaaaagt tcttgtccgt
       61 gttgacttca acgttccttt gaaagacggc gtgatcactg acgacactcg tatcaaagca
      121 gcattgccaa caatcaacta cgttctagaa caaggcggaa aagcaatcct tttctctcac
      181 cttggacgtg taaaaacaga agaagataaa gaaggaaaat ctttagctcc agttgcaaaa
      241 cgtctaggcg aattattagg taaagaagta acattcgtac ctgaaactcg cggagaacaa
      301 ttagaagaag caatccgcaa catgaatgac ggcgacgttg tcgtattcga aaatacacgt
      361 tttgaagata ttgacggtaa aaaagaaagc aaaaatgata cagaactagg taaatactgg
      421 gcttcattag gcgatgtatt tgttaacgat gctttcggta cagctcaccg tgcacatgct
      481 tctaacgtag gtatcgcttc aactggtatc ccaacagttg ctggattctt gatggaaaaa
      541 gaaatcaagt tcatcggtga agcagtagaa gaaccaaaac gcccaatgat tgcaatcctt
      601 ggtggcgcaa aagtttctga taaaatcggc gtaatcgaaa acttgattcc taaagcagat
      661 aaaatcttaa tcggtggcgg aatgacttac acattctacg aagcaaaagg aatcaagatc
      721 ggtaactctc tagtagaagc tgataaagta gaactagcaa aagaattaat cgaaaaagca
      781 ggcgacaaac ttgtcttgcc aatcgacaat gtctgtgcac cagaattttc aaatgatgtt
      841 gaaacacaag taatcgaagg agacattcct gatggtttga tggctttaga catcggccca
      901 gcatcagtaa aattatttgc tgatacatta caaggtgcaa aaacagttgt ttggaacgga
      961 cctatgggtg tatttgaaat gagtaacttt gctaaaggta caatcggtgt ttgtgaagca
     1021 atcgctaact tagaaggcgc aacaacaatc atcggtggcg gagactcagc tgcagcagca
     1081 gaacaattag gattcgctga caaattcaca cacatctcaa caggtggtgg cgcaagcttg
     1141 gaattgcttg aaggtaaaga attaccagga ctagcagcaa tcaacgataa ataaacgacc
     1201 aagtttgctc aattctgacg agtaatcgat caaaaggtga agggagtttt catgactccc
     1261 tagccatttt tcaataaatt aaggagtgtg ttcctcaatg cgtaaaccta ttatcgcagg
     1321 taactggaaa atgaacaaaa ctgcatcaga agcaaaagaa tttgctgaag cagtaaaaaa
     1381 caaaatccct tcaaatgaca aagttgattc agtcatcgga tctcctgcat tattcttaca
     1441 agaattaatg acatcagctg aaggtacaga actaaaaatc tcagcacaaa actgctactg
     1501 ggaaaactca ggtgcgttca ctggtgaaac ttcaccagca gctcttgctg atctaggtgt
     1561 tcattatgtg atcatcggac attcagaacg tcgtgaatat ttccatgaaa cagacgaaga
     1621 catcaataaa aaagcaaaag ctatctttgc aaatggtatg acaccaatcc tatgctgcgg
     1681 cgaatcttta gaaacctatg aagcaggcaa aactgctgaa tggatcgaag gacaaattac
     1741 agctggttta gctgatcttt cagctgaaca agtgtcaaac atggttattg cttatgaacc
     1801 aatctgggca atcggtactg gtaaatcagc tgacgcacaa atcgctgacg aaatttgtgg
     1861 agttgtacgt caaacagttg aaaaacttta tggaaaagaa gtatcagaag cagttcgtat
     1921 ccaatacggc ggttctgtaa aaccagaaaa tattgctgaa tacatggcaa aagaaaatgt
     1981 tgacggtgct ttagtcggtg gcgcaagctt gcaagcagat tcattcttgt cattgttaga
     2041 cgctgtaaaa taattgttga aaatttgtga aaaatgcaag aaatgtttgc actaatgagc
     2101 aaaattaact aaaatcgtat atgagggaaa tcccttagga aaaaacaaac tcaaaggaga
     2161 gacaaaacat gtcaattatt actgatgttt acgctcgcga gatcttagac tcacgtggta
     2221 acccaacaat cgaagtagaa gtatacacag aaagcggagc ttttggccgc ggaatggttc
     2281 cttcaggtgc ttcaactggt gaatacgaag cagttgaatt acgtgacgga gacaaagctc
     2341 gttatggcgg taaaggtgta actaaagctg tcgacaacgt aaacaacatc attgctgaag
     2401 caatcattgg ctacgatgtt cgtgaccaaa tggctatcga caaagctatg atcgctttag
     2461 acggaactcc taacaaaggt aaattaggtg cgaacgctat tttaggtgtt tctatcgctg
     2521 ttgcacgtgc agctgctgac tacctagaag tacctttata ccactaccta ggcggattca
     2581 acacaaaagt attgccaaca ccaatgatga acatcatcaa cggcggatca catgctgata
     2641 actcaatcga cttccaagaa ttcatgatca tgcctgttgg cgctccaaca ttcaaagaag
     2701 ctctacgtat gggtgctgaa gtattccacg cattagcttc aatcttgaaa gctcgcggat
     2761 tagcaacttc tgttggtgac gaaggtggat ttgctccaaa ccttggttca aacgaagaag
     2821 gttttgaagt aatcatcgaa gcaatcgaaa aagctggcta tgtacctggt aaagacgttg
     2881 ttcttgctat ggatgctgct tcttcagaat tctacgacaa agaaaaaggt gtttacgtac
     2941 tagctgactc aggcgaaggc gaaaaaacaa ctgacgaaat gatcaaattc tacgaagaat
     3001 tagtttctaa atatccaatc atctcaatcg aagatggatt agacgaaaac gactgggacg
     3061 gattcaagaa attaactgac gtattaggcg acaaagttca attagttggt gacgacttgt
     3121 tcgtaacaaa cactcaaaaa ttatctgaag gtatcgaaaa aggaattgct aactcaatcc
     3181 ttatcaaagt gaaccaaatc ggtacattaa cagaaacttt cgaagctatc gaaatggcta
     3241 aagaagctgg ctacacagca gttgtatctc accgttctgg tgaaacagaa gattcaacaa
     3301 tctctgatat cgctgttgca acaaacgctg gccaaatcaa aactggttct ctatcacgta
     3361 ctgaccgtat tgctaaatac aaccaattac tacgtatcga agaccaactt ggtgaagttg
     3421 cagaatacaa aggtttgaaa tctttctaca acttaaaaaa caaataattc ttttctaatg
     3481 aagatacatg atcgactatt gaaagatagt cgagtatgat cacttagtta agtaatcgaa
     3541 ctctctactt gctgaaatat cagcaggtag agagtttttt tgcaactagc ttctgtatat
     3601 gatgtttaca aagaatggta caagaaaaga tcatccttga aaaaggaaag ctaacaataa
     3661 ctagcatcat ataagagctt ttaaggatag aacagtagaa taggaatcta tgagcaagtc
     3721 ctctcgtata aataaaatag gagggaagta gcagcatatt tttcataaag tggctctctt
     3781 tcttcatttt tactttggtt gctcctattt gaaaattcga gcacactcaa atacaataaa
     3841 aagaaatggc aagggagagg attagatgaa aaaagaagaa aaaatcgcga ttttacaaga
     3901 gatcatccga attaaatcag tgaatggaaa cgaaggtgaa gtagcagcct atttgaataa
     3961 gcttttggca aaacatgaca taacaggaga gattgtttct tacagagatg gtagagataa
     4021 tttgattgct cgttaccaaa agggccaatc aggaaaagtg ttaggcctat ctgggcatat
     4081 ggatgtcgtg gcggctggtg atgaatcgtc ttggacctat gcaccctttg cagcagaaat
     4141 ccatgggaac cgtttgtacg gacgtggtgc tacagatatg aagagtggct tggcagcaat
     4201 ggtgattgcc atgatcgaac tgaaagagtc aggaaaacca tttaacggaa cagtcaaatt
     4261 attagccact gtcggagaag aagtgggtga actaggtggg gaacagctca cgaaagcagg
     4321 ctatgtagat gacttagatg ccttgatcat cggagagcca accaattaca gtttgatgta
     4381 tacgcacatg ggatcaatca actatacagt cacctcgcac ggaaaagaag cgcatagctc
     4441 tatgccagac caaggttata atgcaatcaa tcacctaaat gaattcatta cgaaagcaaa
     4501 tgctgaaatg aatcacctcg ctgaaacaat cgagaatcct gtcctaggaa aaacaatcca
     4561 taatgttaca ttgatcagtg gcgggaatca agtcaacagc atcccaagcc atgcgcagct
     4621 ccaagggaac attcgatcga ttccagaata cccaaatgat aaaatcattg cattgctgca
     4681 atcaatcgtc aatgaattga atcaagagac agactatcat ttggaattga tgatcgatta
     4741 caacaaaata cctgtcaaag ctgatccaga ttctcctttg attcattgca ttcagcaaca
     4801 attcagccag ccattacctt tagttggagc ggcagcaaca acggatgcag ccgaatttac
     4861 gaaagctaac cattccttcg actttgtcgt ctttgggcct ggtgtcgtta ctttgcctca
     4921 tcaagtagat gagtatgttg aaatcgataa ttatttggat atgatcgaaa aatatcaagg
     4981 aattatttta tcctacttag cgtaagattt ttttaataaa tgaaaaaaca gagccagatt
     5041 agcattattt tgctaatcca gctctgttta ttcagcctat ttttcttttt gaatatatca
     5101 agcgttgtgg caacatagtg atgactaatg aaacaacgac gatagacaac aaacgatcaa
     5161 ggtagtctgt acctgcctgt accaagatca cacttgttgt ctgacttaaa cctaacccat
     5221 gtaatagctg aacgatcata ctggatcctg aggaggtgat cccgccaaat aactgaacgg
     5281 taatgatcga agaaacgatc gttccaggta ccgacaaaat caaagcgagt aataacagtt
     5341 tcaagctgtt tttatctttg ccgagatatc ttggcaaaat caatccagca agcaagcctg
     5401 tgagaatttg gattggcgaa taatacagag aaaaaatatc tgtagtcact ccacttaaaa
     5461 taccgctgga caatcctgtc agcattccag gtacaggccc aagcaatgca ccagcaaaaa
     5521 tcgttccgat agaatcgaga tagattggca aacgaagcca taaagcaatc gttcctccaa
     5581 tataattgat cccaatacac aaagccatca ctgtaagtac gcgagttgcc attttgtttt
     5641 tattcatttt gttagcccat ctttaacctt tccaaatctt tcataatcgt ttcttcttgt
     5701 gcatttaaaa gaacggttaa aaactttcgg aaaaaaagac gagtatctac ttttgtcata
     5761 attttactgt tagcaggttt ctgccaaaat tcataacgat caaccaatgt ttgtccacga
     5821 cttattcctt gagtttccac agcagtgaag cttttgaaac cttcacagat ggcctcctcg
     5881 ataaaataag caacagctaa cggatcattg atgacacatc ctaaaattct ttcttgtttc
     5941 cagtggaagt caaaataaaa acgagtgatc gcttttagat actctccctc ttcaggattc
     6001 atttgacagc agtattcgag gatagtcggt gtcaagacga tttctcttgt cacatctaac
     6061 ccaaccattt ctatcgtttg tttgagattc tcgaatacat aagaagctgc ttcaggatca
     6121 caccagtaat tgtattcagc aactggcgaa cagttaccat ggcttttgta agttccaccc
     6181 atcgagacaa aacgttccat atgtttccca atatttgggt tgactttcaa agcagaagcg
     6241 atattggtca aaggacccaa agcaatgact gatgtatttg tcttttcttt gaaataatca
     6301 gccaaaaaat caacggcatg gagaggttct gcctgttttc tcaagatcat cgggaaattt
     6361 gtttccccta aaccatccat accatgagtg tcttgtgcac tgatgaaagg ttgcttcaaa
     6421 ggtttttcca ttccttgata aacaggaatg tctaaacgat cacagcgttc caggcatttc
     6481 agcacattct gcgtacctat atctgcaggg acattcccac aaactacggt aatagcaact
     6541 acttcgatat caggggattt gacagctaat gataaagcta aagtgtcgtc gatccctgga
     6601 tcgcaatcaa taattacttt tcgcattatt tccaatctcc tttctttaga gatacttagt
     6661 tttttattct gggaagtttc cgaaccagtc caagacgagc gtcttgatta taatgcacga
     6721 aattagtata ctggctttta ttgcgaatgt aaatgctctc ttacaagtaa atctattgag
     6781 gagaacttta agtaaatgat acaatgaaaa agaaatggaa tttacgtgtg ccgaaaataa
     6841 agaatcctat ggatcggtac attggataat agatagatta tagtttggca gaaagcggcg
     6901 ggtgtacaat ggttattttt tatttcattc ttattctgat aattataggg atcagttacc
     6961 attttctttc ttatttttat gcaaagaaat tagtaagagc gggagtacag agaggatatg
     7021 actggtatga agacactggt catcgtttga tggaccctgc tgcagtcacg tcagtagaaa
     7081 aaaaacgaca aaaattagaa gaagagtgtg aaagcttttg ggagcaggga acacccaaaa
     7141 caatcaaaag ttatgacggg ttgaaactag ctggtcagat gttcatccat ccaaataagc
     7201 agaataaatg ggtcatttgc gtacatgatt accgaagcac agggaaaaga gacatgtctc
     7261 atataggtaa aagatatgca gagaaaggat tcaacgttct tgtacctgat ttacgtgcgc
     7321 acggagaaag tgaaggagaa atcatcggaa tgggatggct agatcggcta gatttaattg
     7381 cctggatcca actcatctta gatgaacagc cgaatgcgtc cattatttta cacggcggat
     7441 caatgggagc ttctacgatc atgatggcaa gtggtgaaaa acttccatca gcggtcaaag
     7501 gatttatttt agacagcgga tatgtttctg tttatgcaga attccgatat atgttaagta
     7561 aaatcactgt ctttcctaaa aaaatggtca tgagatatgc gaatcattat gcccagaaat
     7621 acgccggtta ctccttaaag caagcatcag ctacgagaca gcttggcagc aatcatctgc
     7681 ctttattagt catccatggt gaaagagatc attttgtccc tacagaagca gcgtacacga
     7741 ttcaaaatgc tacggcaggg gataaagcac tgctgcttgt tccggaagca gaacacttgg
     7801 aagcatcgat gaaagatccg aaaacctatt ggacagtgat tttttcattt attgaacaaa
     7861 gaataacgat gaattaattt tctcttcttt tttgtaagta attcgaggat aaataattgt
     7921 ttatagatca ggagactgct aaaataaggt aggaataaaa aaatatcagt tatttgagga
     7981 gtgcggcata tggaaatcaa agaagaaaaa atcgtcttgc tttattcaat gatgaacaac
     8041 aagagatcgg agaaatgaca tggtcagatg cgggcccaga catcatgatc attgaccata
     8101 catttgtaga tccagcatat cgtggtcaaa agttagctga aaagctagtg tatactggag
     8161 tagaattagc aagacgagac gggaaaaaga tcattccttt atgcccttat gcaaaaaaag
     8221 agtttgaaaa gaaaccagaa tatcaagacg tcttacgtca gtcataagaa taacagctga
     8281 aagttttgaa ctttcagctg ttattccatt tatatagagt tgtcacggac ttttatctat
     8341 gataaagtta aagagacgat atcttttggg aaggaggctg cgatatgtat aatttaattt
     8401 tgggaatcgt gatcgtttta tcgattgtta tggtcattgc gattatgatg cagccaagca
     8461 agcaaaatag tgcagcgagt gcatttaccg gcggtgcgga tcaactattc ggcaaacaaa
     8521 aagctcgcgg ctttgaagct gtcatgcagc gatcaactgc agttatggga gctatctgga
     8581 tgatcttatt atttgtccta gcatatttat cttcgaagta gttgaataag cctctcgcaa
     8641 aacggcgggc ggcttttttt attgcaaaga gaacgcttaa gaagaagtta tgaaagaaac
     8701 tatggtaaaa tgaggcgaaa gaggtgagac gagtgagaca attaccaaaa cccctttatg
     8761 caaaacatgg aaagcgagcg gttttgctgc ttcacgctta ctcaggaagt ccaaacgatg
     8821 ttcgaatgct cgcacgattt ttggaaaaag cagattatac agtttatgcg ccgcttttta
     8881 aaggacacgg aacaatggag ccatatgata tcttgcaaga aaaagcagaa agctggtggg
     8941 cagacactaa aaaagccatc cattttttac agtctgagca gttttctgat attgccgtat
     9001 taggattatc aatgggcggc atctttgctg tacgagcact agaagaagaa tcagtgattg
     9061 gcggcggttt tttctgttcg cctttgtctc ctgtaaaaac aaacgtgcct gaaaactttg
     9121 aaaaatacgt acgtcaagtg ctgaagacag caggtaaatc agagaaagaa atcaatgaaa
     9181 aagcagttgc ttaccgctcg ttagctgaac aacaattaat ggatatacaa gatcaggcag
     9241 caatcgtaga aagtaggttg tcagatatcc aacagccgat atttttagct caagctggga
     9301 aagatgaaat gatcgaccct aatggcgtat ttgaaactgc acgcaagttg agccgtcagc
     9361 gagtgacact tcaatggtat cctgaaagtg gtcatgtgat tacagttggt acagcaagac
     9421 gcgaattaga aaaagacgtc ttggaatttt tagaaaaatt accttggaat gaggaataaa
     9481 tatgacaaaa gaaacgataa aagagaaaat tctctttttc atggaaagca gtcgcaaacg
     9541 cagtttttct atggaagaaa tcgctgaagg tttagggttc caaaaaagcg atgattttaa
     9601 attactagta caaacgattg ctcaaatgga aagagaacag agtatagttt tcaataaaaa
     9661 aggaaaagtg aaacttcctt taaaaccaat cttgatcgaa ggaacatttc gagcaaatga
     9721 acgaggattt ggatttgtaa cgattgatcc cgaagaagat gacatctata tcccaaaaga
     9781 agcgactggt tttgcaatgg atggagacac cgtagcaatc gatatcatga agcgagcaaa
     9841 tacagatatg gatcgcggag cagaggggaa agtagtcgag attcgagacc gggcgactac
     9901 tcagttagca ggggaattcg ttgcttattc agaagaagaa atacaagaaa cagatcttta
     9961 cggcgttgtt atcccgaaag atagaaagct caaccaattt aaagtatatg cagcagcaga
    10021 gggagttcgt ccagttgacg gaagtattgt gctgatcgaa ctgacccatt accctgaaaa
    10081 agggtatgcg actagtttag aagggataat caagcaagtg attggtcata agaatgaccc
    10141 tggcatggat atcctctcaa ttgtcgtggc taatggtatc cctacgaaat ttcctgatga
    10201 tgtattgtct gaagcagatg aagttccaga tcaaatcagc gaaaaggata taactggacg
    10261 tagagattta agaaatcagc tcattgttac gattgatgga gaagatgcaa aagatctaga
    10321 tgatgcagtt acggttaaaa aattagataa cggtaattat tttcttggag tacacattgc
    10381 agatgtttct tactatgtga cacaaggcag tcaattggac agagaagcat atgaaagagg
    10441 gacaagtgta tatttgactg atcgagtaat tccgatgatc ccgcaacgtt tatcgaatgg
    10501 tatctgttca ctgaatcccc atgttccaag gctgaccatg agttgcgaaa tggagattga
    10561 tccaaatgga caagtagtct ctcatgatat tttcccaagt gtgatccaaa caacagaacg
    10621 gatgacctac acagctgtca atcaaatctt agaagatcaa gacgaagaaa cgatgcaacg
    10681 ctatgctcag ctcgtaccta tgttccaaga aatgggtgaa ttgcaccgaa tccttgaaac
    10741 gatgcgaatc aaacgtggcg cgatttcttt tgaggatcgc gaagcgaaaa tccttgttga
    10801 tgctgaagga cacccaaatg acatccagct aagaagcaga ggaattggtg aacgcttgat
    10861 cgaatctttc atgcttgcgg ccaatgaaac ggtagctgaa cattataata aattaaatct
    10921 gccgtttatt taccgaatac atgaacagcc aaaagaagaa aaaatgcagc gcttctttga
    10981 ttttgcttca gctttgggca ttctggtccg aggaacgaaa ggaacgatca caccaaaaga
    11041 tttacagcat gtgatcgaag gagtagaaga caaaccagaa gctgcagtta tcaatactat
    11101 gttgctaaga agcatgcaac aagcacgcta ttcagaagac aattttgggc attatggctt
    11161 agcggcagat tactacacac actttacatc tcctattcga cgctatcccg atctaatcgt
    11221 ccatcgtctg atccgttctt acagtgaaga taaaagtgaa agcaatcaaa gttcgtgggc
    11281 tgcggaactt cctgaaattg cagatcatag ttctaagatg gaacgtcgtg cagtagaaac
    11341 agaacgtgaa gtagattcca tgaagaaagc agaattcatg atggacaaag tgggagaaga
    11401 attcgatggg atcatcagct ctgttgtgaa attcggcctg tttatcgaac tgccgaatac
    11461 agtagaggga ttgatccata tcaatgaatt gaaacaggac tacttccatt ttgttgaaaa
    11521 tcatttagct cttgtaggtg aaagaactgg tctgactttt aaaatcggac agaaagtgcg
    11581 agtaaaagta gtcaaagctg atccagatga acgagcagta gattttgaac ttgtttccgc
    11641 agaagaggtt acagcgttgg aacggccaaa aacacgaaat ggaaaatcag gaaaacgcga
    11701 aggaaaacaa gcaggaaaac gcccagaaag aaatggcaag aagaacaaac gatatagcga
    11761 tagtaagcaa acgttcggta agaaaaaagg gaagaagcca ttttacaaag gtgttaaaaa
    11821 aaagaaaaag aaaaaataaa atgagttgga ggaaacagct atgccaaaag gcgagggaaa
    11881 attaattgca caaaacaaga aagctcgcca tgattattcg atcatcgaca cgatggaagc
    11941 agggatggtc ttgcaaggaa ccgagatcaa gtcgatacga aacagccgga tcaatctaaa
    12001 agatggattt attcgcgtcc gcaacggaga agctttcttg cataatgttc atatcagtcc
    12061 ttatgaacaa ggaaatattt ttaatcatga tccgttgcgc acgagaaagt tattattaca
    12121 caaaaaacaa atcatccggc ttgaaaatga attgaaaaat actggaatca ctgttgttcc
    12181 tttaaaagtc tatattcgta acggctatgc caaggtattg attggtctgg cgaaagggaa
    12241 aaaatcttat gataaacggg aagatttgaa acgaaaagat atcgatcgac aaattgatcg
    12301 aacattaaaa aatttctcta gataatttca ttatcctttt tcgcatattc ggtgcttttg
    12361 cttttactta atgataaaat ttgttagcat ttaatctgat aaaacatgat tatcataggt
    12421 tttttcttag atcaggagca ccgtgtataa agaaaaattc gcttcgtttt tgactgaaat
    12481 aaaaaagtgt ctaattttca tgaaaatgtt ttgaattttc tgtttcatgg tggtatgata
    12541 ccgtgtggtt agaaaccgat tggaaaaaac cagatacgca catgaattga ttttatgtga
    12601 atttcgggga aaagaggtga aatggattgg atgaacgctc gctaacgttc cacattggac
    12661 caatttggtt tgatggtaca gtttgtataa tggtgttact gacctgtttg attgttttct
    12721 tcttagctta cttttttaca agaaatctta agttgaaacc aacaggcaaa caaaatgcga
    12781 tagaatgggt cattgatttt agtcgaggaa tcgtaacaga taatctgcct agaaaagaat
    12841 tgagcaattt ccatttgttg gcatttacat tgtttttatt tgtttttgtt gcaaacaaca
    12901 tcggtttagt aacaaaaatc gttttaccta acgaaacaac actttggaaa agcccgacag
    12961 cagacccgtt tgttacttta actttggcat ttataatgat cttacttact cacttatttg
    13021 gggtgaaaaa gttaggcttc aaaggatatt tcgtaaattc gttcttgaaa ccatatagtt
    13081 ttatgtttcc gatgaaactg attgaagaat ttacaaactt attaacactt gctttacgtc
    13141 tttatgggaa tatttatgcg ggggaagttt tgctaacctt gattgcaaaa atggtaataa
    13201 gcttaggctg gttctcattg ccgttagcaa ttccattaga gatggtctgg atcgcattct
    13261 ccttgtttat cggaagcatc caagcatttg tcttcgttac tttatctatg gtttatatga
    13321 gccataagat tgaagtggaa gaataatatt cgttttaaaa acaaactact atttatctaa
    13381 tttaggagga aaacaattat gaattatatc gcagcagcaa tcgcaatcat gggagcagca
    13441 atcggagccg gttatggtaa cggtcaagtt atctctaaaa caatcgaatc aatggctcgc
    13501 caacctgaaa tgtctggtca attccgtaca acaatgttta tcggggtagc tctagtcgaa
    13561 gctgttccta tcttaggggt agttgtcgcc ttgattctag tttttggtgt ttaattgaat
    13621 cagtattaag gcagaaacaa tttggtttgt tactgctttt ttacatgaaa actagaagta
    13681 cttcaaagaa aggaaggctc gcctatgctg aatcaattgg caattgcaga agttggcaat
    13741 gcgatgttag gaaatgtcat cgttgtcagc ggctcatttt tgatactact ggctctcttg
    13801 aagcattttg cttggggacc aatcagcgat attttgaaaa aacgtgaaga caagatcgcc
    13861 aatgatttgg attctgcaga acaatctcgc atcaactcag cgaaaatgga acaagaacgc
    13921 gaacaacaat tgttagcctc tcgttctgat gcagctgata tcatcaaaaa tgcgaaagaa
    13981 agtggagaat taagccgcca aaatattttg aaggatgctc aagaagaagc agctcgtcta
    14041 aaaagtaaag cccaagctga tatcactgta gaacgtgatt cagcgctgaa ctctgtaaaa
    14101 gacgatgttg cagaactatc tcttcaaatc gcggaaaaaa tcttgaacaa agaattatct
    14161 ccagaaatgc acgaatcatt aatcaatcaa tatatcgaag gtctaggttc ttcaaatgaa
    14221 actagataaa tatacagtag gaagacgtta cgggaaagct ttatttgaac tggcgatcga
    14281 ttcaaatcaa gcagaagaaa tctatcaaga tctcgtaagc ttacgtcaaa tctatgaaca
    14341 agtacctgga ttaggtaata tgttaagtga tgttcgtcta gaacctcatg aaaaacgaga
    14401 aatcatggac aagcttgtta gtggctatga tggaatcgtt aaaaactttt tagaagttgt
    14461 ttatgcgtat aaccgaatgg atgatctttt gttcatgatc gaagaatatg aacgtcgata
    14521 tgatgaacat caaggtttgc tgttgggaag tgtcacaaca gcagttccac tttcagacga
    14581 acaacgaata caattagaaa agaatgttgc aaaaacaatg aactatcaat cagtcgaatt
    14641 aaaacaaatc gttgattctt ctattattgg tggcgcgatt gttgaagcta accatcgagt
    14701 aatcgacgga agcattcgca cgcaattaga aaatatgcgt aatcaattaa atagatagta
    14761 caaagaggta ggtgaatcga atggccatca aagcagaaga aattagtgcc ttgattaaag
    14821 aacaaattga aaattatcaa aacgtgcttt cagtcgaaga aatcggtacc gtaacttatg
    14881 taggtgacgg gatcgctcgt gcacatggtt tagaaaacgc gatgagtggt gagttgcttg
    14941 aattttcaaa tggctcatac ggaatggcgc aaaacttaga atcaaatgat gtaggtatca
    15001 ttatccttgg cgatttcgag tccattcgtg aaggagataa agtaaaacga acaggaaaga
    15061 tcatggaagt tccagttgga gaggccttga tcggacgggt agttaatccg ctaggtcagc
    15121 caatcgacgg actaggtgaa atcgttactg acaaagctcg tccagtagaa gcaatggcac
    15181 caggcgttat gcaacgtaaa tctgttaacg aaccaatgca aacaggtcta aaagcaatcg
    15241 acgctcttgt accaatcgga cgtggccaac gtgaattagt tatcggtgac cgtaaaacag
    15301 ggaaaacttc tattgcgatc gatacgatca tcaaccaaaa aggtcaagat atgatctgta
    15361 tctatgtagc aatcggacaa aaagattcta cagttcgtac acaagttgaa acattgaaaa
    15421 aatatggcgc aatggattac acaatcgttg tgaatgccgg tgcgtctcaa ccagcgccgt
    15481 tgctttatat cgcaccatat gctggtactg caatgggtga agagttcatg tacaacggta
    15541 aacatgtctt gatcattttt gatgatcttt caaaacaagc tgttgcttat cgtgaacttt
    15601 ccttgctact tcgccgtcca ccaggtcgtg aagcttatcc aggggatgtt ttctacttac
    15661 actcacgctt attggaacgt gcagcaaaat taagtgatga tttaggtggc ggttcaatga
    15721 ctgccttacc atttgttgaa acacaagctg gagatatctc tgcttatatc ccaacaaacg
    15781 ttatttcgat cactgatgga caaatcttct tagaaagcga cttgttctac gcaggtactc
    15841 gtccagcggt tgatgccgga ctatctgtat ctcgtgtagg gggttctgcg caaatcaaag
    15901 caatgaaaaa agttgctggg actttacgtc tagacttagc aagttaccgt gaattagaag
    15961 catttacaca attcggttct gacttggatg cagcaacaca agcaaaacta aatcgtggac
    16021 gccgtacagt agaaatcttg aaacaaaaat tgcatgcacc gctagctgtt gaaaaacaag
    16081 tagtgatttt gtatgctttg acacatggat tcttagatag tatccctgta gacagtattt
    16141 tagacttcga acatgaatta ttcgaatatc tggataccaa tcattcagat atctttgaaa
    16201 caatccgtac aacaaaagat cttccagaag aagaagcgtt gaacagtgcg attcaggaat
    16261 ataaagacat gttcttagct acaaaaggaa ataattcctc tacagaagat aaattgaaat
    16321 ccattcaaaa tgcatagtga ggtgagatga atgggtgctt cattaaatga aatcaagaca
    16381 cgtatcgctt caacgaaaaa aacgagccaa atcacgcggg caatgcagat ggtgtctgct
    16441 tctaagttga caaaatctga agcttcttca cagaagtttc aggtttatgc aacaaaagta
    16501 agagaaatcg ttacgcattt gactgctact cagttaaatg atattgcctc agaaaatcct
    16561 agaggggata tcaattacaa cagcatgctg ctcagccgtc ctgttaagaa aactggttat
    16621 atcgtcatca ctgcagatgg cggtttagtc ggtggatata acagctcgat attgaaacaa
    16681 acgatgtcta ttttagaaga agaccaccaa tcacccgatg attacgtgat gatcgcaatt
    16741 ggcggaactg gtgcagactt ctttaaagca cgcggtatca atctagcata tgagctgcgc
    16801 aatctatctg atcagccaag ctttgatgaa gtccgcaaaa tcgtaaatat ggcaacaact
    16861 atgtaccaaa acgaagtatt cgatgaatta tacgtatgtt ataaccatca tatcaattcg
    16921 ctaactagtc agtttcgtgt agaaaaaatg ctgccaatct cagacttaga tccagaagaa
    16981 gcaactactt tcgaacaaga atatattttt gaaccttcaa aagaagaaat cttgtctcag
    17041 ttattgcctc agtatgctga aagtttgatc tacggtgcga tcgttgacgc caaaacagca
    17101 gaacatgctg ctggtatgac agcaatgaaa acagcaactg ataatgctgc taatatcatt
    17161 gatgagctga cggtttctta taaccgtgca agacaagggg ctattaccca agaaattacc
    17221 gaaattgttg ccggagcttc ggcactagaa tagtattgag attggaggaa aaaagatgag
    17281 ttcaggcaag attgttcaag taatcggtcc cgttgtcgac gtggaatttt ctttagacca
    17341 atccttacca gatatcaaca acgcattagt tgtttataaa aatgacgaaa ataaatcaaa
    17401 agttgttctt gaagcagcgt tagaattagg ggacggagtg atccggacca ttgcgatgga
    17461 atcaacagat ggtttacaaa gaggaatgga agtcattgat acaggcaaag caatctctgt
    17521 tcctgtaggt aaagaaacat taggtcgcgt attcaacgta ctaggagata cgatcgattt
    17581 agaaacacct ttcccagaag atgcggaaag aagcgaaatt cataaaaaag caccagcctt
    17641 tgacgaatta agtacaagta cagaaatttt ggaaacaggg atcaaagtta tcgatttgct
    17701 tgccccatat ttaaaaggtg ggaaagtcgg actattcggt ggtgccggtg ttggtaaaac
    17761 cgtactgatc caagaactga tccataatat cgcccaagaa catggtggta tttctgtatt
    17821 tactggtgta ggtgaacgta ctcgtgaagg taatgacttg tattatgaaa tgaaagattc
    17881 aggagttatc gaaaaaacag ccatggtgtt cggacaaatg aacgaaccac caggtgcacg
    17941 tatgcgtgtt gctttgactg gtttgacgat tgcggaatat ttccgtgatg tagaaggtca
    18001 agatgtactg ttgtttatcg acaacatttt ccgtttcact caagctggat ctgaagtatc
    18061 agccttgtta ggacggatgc cttctgcggt tggttatcaa ccaacattgg caacagaaat
    18121 gggtcaattg caagaacgta tcacatctac gaaaaaaggt tcgatcacat cgatccaagc
    18181 aatctatgtg ccagccgatg actacactga cccggcgccg gcaacagcat tcgcccattt
    18241 ggatgcgaca accaacttgg aacgtaaatt aacagaacaa gggatttatc cagcggtaga
    18301 tccattggct tcttcttcaa gtgctttagc acctgaaatc gtgggagaag aacattataa
    18361 agtagcaaca gaagtccaac acgtattgca acgatatcgt gaattacaag atatcattgc
    18421 tatcttaggt atggatgaat tatccgatca agaaaaagtg ttagtttctc gtgcccgcag
    18481 agtccaattc ttcttgtcac aaaactttaa cgtggctgaa caatttactg gtcttccagg
    18541 ttcatatgtc cctgtagaag aaacagtgaa aggattccgc gaaatcttgg aaggcaaata
    18601 cgatgatctt cctgaagaag ctttccgaag tgttggccga atcgaagacg tcgtagaaaa
    18661 agcgaaaaca ctaggctact agaaaggggt gccccatgga tcaatattta acagtaaatg
    18721 tggtaactcc tgccggtttg gtctatgatc accatgcagc tatcgtcgtt gcacgtacga
    18781 cagctggtga gcttgggatt ttgcctaaac atgcaccaat catcgttcca ttgacgattg
    18841 atgaagttcg tgtaaaacga acagattcag atacccacgt cgattggatc gctgttaatg
    18901 gcggaatcat ggaagttcgt gataatgttg tttcaatcgt tgctgatagt gcggaacgtg
    18961 aacgtgatat cgatgtatct cgtgcggaac gtgcaaaaca acgcgcagaa cgccaaatcg
    19021 aagaagcaaa agcaaaacaa gatacgaacg aattgaaacg ggcaactgtt gcgttgcacc
    19081 gagcaatcaa tcgtatcaaa gtatctaagc actcataaaa tgagatgcgg atgtttatcc
    19141 gtgtttaaga acagtaaaaa gaggttatga cagaagtggt cagcttcaag acataagaag
    19201 gaatttacga aaattgcttc tcaaattttt gtgaaatctc agcttatttt tcgaagaagc
    19261 tacttctgga acaccgtata tcagaggttg taaaatcagc gttttgtcct gagtattaga
    19321 gaaacaaatg gaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca
    19381 gatgattgaa caccgtttat tt
//
LOCUS       DAMEET010000055        19079 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.056, whole genome shotgun sequence.
ACCESSION   DAMEET010000055 DAMEET010000000
VERSION     DAMEET010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.056"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            647..964
                     /locus_tag="PB780_001720"
     CDS             647..964
                     /locus_tag="PB780_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDE0733774.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(1055..1420)
                     /locus_tag="PB780_001721"
     CDS             complement(1055..1420)
                     /locus_tag="PB780_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733775.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1628..2110
                     /locus_tag="PB780_001722"
     CDS             1628..2110
                     /locus_tag="PB780_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733776.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            2307..3197
                     /locus_tag="PB780_001723"
     CDS             2307..3197
                     /locus_tag="PB780_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HDE0733777.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            complement(3484..4758)
                     /locus_tag="PB780_001724"
     CDS             complement(3484..4758)
                     /locus_tag="PB780_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDE0733778.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            5031..7502
                     /locus_tag="PB780_001725"
     CDS             5031..7502
                     /locus_tag="PB780_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDE0733779.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(7605..9341)
                     /locus_tag="PB780_001726"
     CDS             complement(7605..9341)
                     /locus_tag="PB780_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDE0733780.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(9338..10042)
                     /locus_tag="PB780_001727"
     CDS             complement(9338..10042)
                     /locus_tag="PB780_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDE0733781.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            10173..10676
                     /locus_tag="PB780_001728"
     CDS             10173..10676
                     /locus_tag="PB780_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDE0733782.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            10636..10974
                     /locus_tag="PB780_001729"
     CDS             10636..10974
                     /locus_tag="PB780_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HDE0733783.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            10995..12413
                     /gene="ffh"
                     /locus_tag="PB780_001730"
     CDS             10995..12413
                     /gene="ffh"
                     /locus_tag="PB780_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDE0733784.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            12525..13103
                     /locus_tag="PB780_001731"
     CDS             12525..13103
                     /locus_tag="PB780_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDE0733785.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            13107..13628
                     /locus_tag="PB780_001732"
     CDS             13107..13628
                     /locus_tag="PB780_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDE0733786.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(13707..14261)
                     /locus_tag="PB780_001733"
     CDS             complement(13707..14261)
                     /locus_tag="PB780_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDE0733787.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            14514..14789
                     /gene="rpsP"
                     /locus_tag="PB780_001734"
     CDS             14514..14789
                     /gene="rpsP"
                     /locus_tag="PB780_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDE0733788.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            14801..15052
                     /locus_tag="PB780_001735"
     CDS             14801..15052
                     /locus_tag="PB780_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HDE0733789.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            15177..15968
                     /locus_tag="PB780_001736"
     CDS             15177..15968
                     /locus_tag="PB780_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733790.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            16138..16659
                     /gene="rimM"
                     /locus_tag="PB780_001737"
     CDS             16138..16659
                     /gene="rimM"
                     /locus_tag="PB780_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDE0733791.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            16649..17410
                     /gene="trmD"
                     /locus_tag="PB780_001738"
     CDS             16649..17410
                     /gene="trmD"
                     /locus_tag="PB780_001738"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDE0733792.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            17546..17893
                     /gene="rplS"
                     /locus_tag="PB780_001739"
     CDS             17546..17893
                     /gene="rplS"
                     /locus_tag="PB780_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDE0733793.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            18151..18444
                     /locus_tag="PB780_001740"
     CDS             18151..18444
                     /locus_tag="PB780_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HDE0733794.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         6346 a         3365 c         3821 g         5547 t
ORIGIN      
        1 ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg tacattatgc
       61 tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac ttaatccata
      121 gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca ccccaaacca
      181 cttccccata agtgtattac gctttaaact cttttatatt tgaattcact aagaagcata
      241 ccatatattt gaatttacta agaagcatac catatttttg aaaaaaagtg agaaaaaagg
      301 cttataatta ggatttagag taattaatta gatgtttatt ttataggact gatactatag
      361 attacaaata gtatcgtatt tcgcaatctt aaaattatct tcttactgtg aataagataa
      421 taaaatttgt atttgtcctt tttaggtaac ttattttaat ctatatacct ttctggatca
      481 acgaaagtat actttatata gtcataacgc cgatggtaac tatgcactgt aacgataatg
      541 ctgctaaaat atctttttac gaaaaacatc acaaggaaaa acttaactat ggtattatct
      601 aaacataatt ttttcggtat ttaatattta attaggaggt aatattttgc ttttttatag
      661 taattttatc ttaatagtag cgatattatt acttctgaat atatggattt ttgataggag
      721 tagaaattca agtataggtt ttagaactaa acgaagtttg tcttctaaaa aaaattgggt
      781 atactcacaa actatttttt atggaggaat tgttttaatt agtttacttt cttcaacact
      841 ttattctttg aatattatag atgtttccac ctcaaattca atatctatta ttggaatcat
      901 tattgctgca attattacac aactgttttt agtgtttgga gagaagaaaa gaagtaaaaa
      961 atagaaaaac tagtgatagt tttggtgata taagaaaaac atatatgtat aaaaagaaga
     1021 aagtagcttc tctttgtaca tatatggttt gtttttatgg atgccgagct tttttataag
     1081 ataaggctac cgcccataac gacccaagga tttgtggaac taagtaaata cctgcaagaa
     1141 gcaagatcag ctgggtcggt tttaaaggat gatagaagta acgccattca gtaaaattgg
     1201 cgcaaaataa ataggaagtg acagtagata taatattggc aatgaataaa gatataatac
     1261 gcctgttcct ttgaaaataa tatccagcaa aaagagataa aaatatcaat gtgatgaacc
     1321 cgcttacgga atgataacga aaatcaagca gtaaggcgaa atgcacataa ggaacaaaag
     1381 ataaaagcaa cggaaggttt ttccagcaag tttttatcat ctttcatcac cacctaacaa
     1441 gtgttattat tttgttagtt ttattgtaaa ttgaatcctg caataatcaa ttactttttc
     1501 attatttcta gattaaatga ataaactctc atgccattat ctctaaaaaa aaagtttttc
     1561 cccctaattt ttttagatag aaaattgtat agtatgatga gagaaaaagt agtttggggg
     1621 aacgttaatg agaagaaaag cagtaatcga tcggctttat ttattttttt ggttttttcc
     1681 tatcgttttg ctacctttta catgggtaaa tgctgttgta caaggtgcgg agatattagg
     1741 cctgatccaa ggaagcggat ttgtggtttc taggaatttt ccagctttga ttgttttcta
     1801 tttagtgata agtgtaggtc tcttattacc aaaatctctg atcaatcact acaattggca
     1861 gctgctttac ttttcttgct tattagtatt tacatctatg ttttctttgc tgcctcgctt
     1921 gattatcggc cctaatggtt tgagcgagag tggattgaag gatttagctt attcctccat
     1981 gtatttcttg acgatcaaac catggttcta tttagtaatt gcctcattta ttcttgcaac
     2041 atttttttac ttctggacag aaatcaggat aaaaagtgaa cgatctgtat ctataaaaaa
     2101 taactattga tggtggatca tcagtaaaaa aacatatgag ctgcgtaaaa tgcatgtttc
     2161 tcatatgctt tttttattat tatgtaaaga atgcgaaaaa cgtttacatg atttttacat
     2221 aacttaacta tatttttaca gcaaccatac gagggattta cagtcgactg ataaacttta
     2281 gttgtaaata agaaaggagc aataaaatga aaaaagtatt gtttactcta ggtatgatgg
     2341 gaattctttt agccggatgc ggctcaggat caggaaatac agatggctct gccacacaaa
     2401 ataattcaag tgactcaaat gaacaagtaa aaatcgttgc ggtaggttcg acagcattac
     2461 agccgttagt cgatgcagca caagaaagct ttgtacaaga aaatccaaat tatcaaatct
     2521 ctgtacaagg aggaggaagc ggaactggtt tgagccaagt cgaagctgga gcagttacta
     2581 tcggaaactc tgatgtattt gcagaagaaa gagacggagt tgatgcttca aaattagtgg
     2641 atcataaagt agcagtggtc gggatggcac caatcgtcaa taaagataca gatgtcaaag
     2701 atatcaccaa acaggaattg attgatatct ttacaggaaa aatcactaac tggaaggaag
     2761 ttggcgggaa agatcaaaaa atcaacgtcg tgaaccgtgc aaacggcagc ggtacccgcg
     2821 cgacattcga aaaatgggga ctggatggtg ctacccctgt gcagtcccaa gaacaagatt
     2881 catcaggaac agttcggcag ctggtaagtc aaacaccagg agcaatcagc tatttagctt
     2941 tctcatatct cgatgattcc acacaagcac taagtatcga cggtgtagaa ccaaaagaag
     3001 agaatgtagc agataacagc tggggaatct ggtcatatga acatatgtac acgaacggaa
     3061 aaccttcccc tgaagtccaa aaattcttag actacatgat gacagaagaa atccaagaag
     3121 gacctgtcaa agaattgggc tatttaccaa tcacaatgat ggaagtagaa cgtgatcacg
     3181 aaggaaatat caaataacta gcagataaac atgccacggt agtcgttact attaaggaaa
     3241 aatcaactat gtttccccac atatacattt ttcctttaat tgtgaaaaat agttcctcat
     3301 attttgccgt tttttgagct aataccgagg gtcagctgat tgaacaccgt ttattcgaaa
     3361 aaaagagcta cgacatagaa ctgtcgtaac tttttttggc tcttcgtcaa atcgtgttgg
     3421 tgaagacaaa tttgaataaa acttagtccg atatgcggcg cgttctgtgc gccgcatatt
     3481 tttttagttt tcaaagatca gaccttgttg taaatagatc cgtgcccgaa agttataaaa
     3541 gttgcgataa ccaaacgcaa ttcgtttaat cagcttgatc ttgttattca gcccctcagt
     3601 gacgccgttt gagtgggaca ccttaaaggc atttgaaata cccgattggt agcaatggaa
     3661 gatcgtgaat ttctttttaa acgactgagg taacgtagat gaaattgttt taagggtttc
     3721 ttgaaagcct tccgtacctc gtttaacgaa ataatagcgt aataattgac aaaaatgata
     3781 cgcagttcgc aattcttcgt tgtagcttaa cagctcatca acgatttctg tttgactaat
     3841 tggtcgttta aacagtcgat gataatgccg tttgagtggc tcccatgtcg tggaatcttt
     3901 gagtaagagt ttccaatatc gcttgagccg acggtacttc ttctgatttt cagagtgtga
     3961 ggttcgaaaa cgattcatga tctggacacg taattgattg aagctgcgat tgatgtgttg
     4021 gacaatatga aagcggtcag ttacgatctc cgcatttgga aagactgttt taagcaactg
     4081 acaatagctg gcattcatgt ccatcactag gtacttgacc tttaaacggg cctttctagg
     4141 aaagcgcagg aagtattgag ttagtttgta aagtctgcga tcttcaagga tcgtaaatag
     4201 ctgattggtt gtcccatcaa cacaaataaa actcatggaa ccagaaacag aattcattga
     4261 tttgaactca tccatacaca acaccgtcgg aaggaagttc atacgtggct ggtaatgctt
     4321 cgttgcttca cgcaagatcc gtgtcaccgt cccatccgag acgaagtact tcttagcgat
     4381 tagtttacgt gattgatttt cgtacagttc catgagaatc gcctgcttca gttcctttgt
     4441 tagttgatga tgttcatcaa ctaagtccgt tttcgcagaa aaggtccttc cacaatcatg
     4501 gcagagaaaa cgggtacgtt ttaattctaa aatagttaac ttttctttga ctttcaacat
     4561 ctgagtgcgt gtcgtatagg aaccgttgcg gataatacga accgatcgac agtgttcaca
     4621 ataggaagaa gagtcatcca attttccttt aataaactgc gcttgaacgc ctttatattc
     4681 tttttcaatc agccattctt catcaaaaaa taaatgttta tctgttaatc cgagtaattt
     4741 tctagtatga ttatccattg agagactcct ttggtaggat gacgttttgg tcgactaaat
     4801 tttaccaaaa gtgagtctct ttttgagttg aaacgaagaa aaagtgttag tagaaaatcg
     4861 ccttcgattt tccaccaaca caaaatatta tagaaccctt tttttttgct atatgacagg
     4921 aatgaaaggt gtttgcaagc ttaatgtgag gaacactttc catatctgtt ctatactgga
     4981 taggagaagt ttggaaatat ccagaaaacg aaatcaatcg aggtagccac atgaagaata
     5041 aatggataaa tcgaagtaaa aaaaagaaag taacacaaag atcatcagat aaagtcatcc
     5101 gtctttttcc atcgtataaa gaaggattaa cggcagatca agtcaaggaa cgaatagaaa
     5161 aaggtgcagc aaataattca gtcgatccca cattcaaaac aaatcagcaa atcgtattgg
     5221 aaaacatatt tacgtatttc aatttgatct ttcttatctt agccattttg ctttgtttag
     5281 tagaatccta taagaacttg acttttttac cagttatcat agccaataca ggaatcgcca
     5341 tttttcagga aattcgatcc aaaaagatct tggacgaact aaatgtttta aatacagcaa
     5401 aagtcaatgt catacgagac gggatagaac aaacaataga tatagaagac cttgtaatag
     5461 atgacattgt tattttagag actggatatc aaattccagc agatgcagag gtcgtggaag
     5521 ggaaactgca agttaatgag gctttattga caggcgaagc agatgaaata accaaagaaa
     5581 taggtgatcc attaatgtcc ggcagtttca ttgtttccgg aaaagcttac gcccgattag
     5641 acaaagttgg agcagattcg tatatttccc agctgacagt caaagccaag acaatgggag
     5701 aaggagaaca gtcggagatg attgcttctc ttaatcaatt gatcaaatgg attgggatca
     5761 tcattattcc tatcggtatc ctgctttttt ctcaaagtta cttttttaat ggaaacacga
     5821 tcaaagaaag tgtcgttgca atggaagcag cattgatcgg tatgatacct gaaggactgt
     5881 acttgctgac gacgattgca ttagcattaa gtgcgactaa actagcaaag caaagagttc
     5941 ttttacacaa tatgaaaagc attgagacat tggcaagggt aaatgtcctt tgtgtagata
     6001 aaacaggaac gatcacagaa aataaaatgt ctgttcaaaa aatgatcgtc tctaaaaaac
     6061 aagaaataat cagtcagtca gatcaattgg aagcatgcat tgctgactat gcaaaagcga
     6121 tggcaggaga caatgcaaca atggaagctg ttaaagcata ttttacaaaa accactgatc
     6181 aatcctatca ccatgttatt cctttttctt ctgtccaaaa attcagcagt gtagcgttgg
     6241 cagacaaggt gtatgtttta ggagcgcccg aaatggtttt gcgcgatcaa ttgccggaat
     6301 atgcggaaga gtttgtttct ttcgctgaaa aaggctatcg cgtgttggtc tttggtgtgt
     6361 acgatgggat tcttgaagaa ccagtattga cagaagcagt cacaccatta ggctatattc
     6421 tgattgctaa tcctatcaga aaagaagcaa gagccacatt tgattacttc aaaaaacagc
     6481 aagtagccat caaagtgatt tctggagata atccgctcac cgtatccaat gtagctgtcc
     6541 aagcagggat ttcacatgct gatcagtacg ttgatgcatc taaattatca gaagaagaat
     6601 atgaagcagc tgtcgaaaaa tatacagttt ttggacgcgt caagccagaa caaaagaaaa
     6661 tcttcgttca attgataaag cgtaacaata cagtagctat gacaggtgat ggcgtgaatg
     6721 atattctagc catgaaagaa gcagactgca gtatagcgat ggcttccgga aatgaagccg
     6781 ccatgcaggc ctcacaagtg gtcttacttg attctgactt ttcacgcatg ccagaagttg
     6841 ttgcagaagg acggcgcgtt gtgaacaata ttgagcgatc agccagcttg tttttagtga
     6901 agaatatttt ctcctttttg ctctcactgt tttcagtgat ctttgctctg acctatcctt
     6961 tagaaccatc acaaatcaca ttgattagtt tgtttactat cggacttcca tctttcttgc
     7021 ttgctctaga agaaaacaaa aagcgaatca gagggaaatt catcatgaat gtgatggaaa
     7081 aagctgtgcc aggtggacta acagatatga tcgtagtcgg tgcgttggtg atctgcgggg
     7141 tgactttgaa tctaaataaa acagatgttt ctacagcatc taccatgctt ttgattgcag
     7201 tagggttcct cgttctctat aaaatctgtt caccgctgaa taaattcaga agtcagatca
     7261 ttcttttctg tgctagcggt aacttttttt cggtcatctt tttgcataag ctgttttcaa
     7321 tcaccggtat ctcagctgta tcacttttgc tgttgagcat tctatttttc tcggcagatt
     7381 cgatttttag gcatttgacc acattggtag aatatttgtt tgaacgtaaa aatgaaaaag
     7441 agcaaaagcc gtttagtcgc gtagctaaaa aatttctatc gatatttaaa cgagaaaatt
     7501 gacgaaaagt atctcaaaaa agagtaaaaa agaacaacca ctcgtgtatc tttcgaagca
     7561 ggatgagtca tccaacttta aaacgcacga gcagttgctc ttttttacat tttcggaatg
     7621 gtaacggtga aagtcgaacc tgttccaaga tgactgtcta cagtaatcgt accattaagg
     7681 agttgcacgt agtcttttac aatcgctaac cctaatccgg tcccgcctga atgtcggctt
     7741 cgcgccttgt ctacgcggta aaaacgctca aagatccgtt cctgatcctc aatgtcgata
     7801 cctataccaa agtcttggac agaaaacgat agatcatctg ttgcctgata gcggataata
     7861 atctcgctgt cgctttttga atattggaca gcattttcaa tcagattttt gataattgga
     7921 taaaggatat ctgttttagt agtaaatggt tcgttctttc caatcaatcg aatcgtcaac
     7981 cgtttctttt cgatgattgg ctgataattt tgaacgattt gctggaaata aggttctgta
     8041 taaattgtct ggatctcgta agaattctcg ccatcttttg acagctggat gatttcccga
     8101 atcagtttat ctaaccggat cgcatctttt tgcatgatct caagaaaaga agtcagagtt
     8161 tgcgggtcat cttttgcacc atccagtaaa gtctcggtaa aaccaatcag tgaagtcaca
     8221 ggagttttta attcgtgaga gacgttgcca acgaaatcct tttgtagctt ttctaaacgt
     8281 cgcactttcg ttaagtcata cgccacccct agaatctgtc cggtaccttc tgaattagta
     8341 aagtagcgca aactcaagtc taatgtctgc cgaccttctg taagggtgat ctcctgatgg
     8401 atcagcggat gctcaggagt cacttgatga atcagctgaa tgagttttgg ctcttgaatg
     8461 acttctgtgt accgctgttc aggttgatag cttaccacgt ttaaatgtag ctgcatcttt
     8521 ggattaagca gtagtaaacg agaatcagca tcatcaatca ggaatacgcc aatcatcagt
     8581 tcgttcagta acgtatataa ttgctcttct gtggtagtac acgcccgata catcttactc
     8641 atttgttcgc tcaacgcatt aatcgtctga tacaattctt cccattgatc gggtgtttgc
     8701 atgattactt ccgcctgatt aggattttgc accatttttt ttagtacggg cagcacggtt
     8761 ttcaatggct ggtttttttg atagatcaga taatagatca ttcctgcaag aacaaggaag
     8821 aaagacagaa agaagaaaaa gacccaacgg cgaaaactct cagttctcgg taaaaaaacg
     8881 cttgtcggtt ctgcgatacg taaaatggct tctaactggc cacttttttt gactggtaat
     8941 gcgacatata aaagatcctc attcaaagta gtacttttgc gcaaagcgct acctaggttc
     9001 ccaccattca atacggcttt gatttcggga cgggagtttc gctgttcatt caatgtttca
     9061 ttgtttgtgt caaataagat ctttccagta gcatccatta aagtaatccg ttcgttcgac
     9121 tgatggacga attcttccaa agcagttttg ttttgcgctg cttggaggtt ttcgacatct
     9181 aattgccgga tcagcagttg tgcttttttt gtcaggtagc tttcttgctg ttccacgatc
     9241 tgctgttgaa aataatggga aatcaattgc cagccaccaa aaaacaaact aactaaaaga
     9301 ataagaaaga agagacgttc ataaattttt ttccatttca tcgtttgggt tcctgaaact
     9361 tatagccaaa tcctctaata gtcaccaagt actttgggtg ttttggatct cgctcgattt
     9421 tttctcgcaa atgactaacg tgtacatcta cgattcggct ttgtcctgcg aagtcaaaat
     9481 tccaaatgcg gtctagcaat gtgtctcggt cgatcacacg gtcttttcgt ttcatgaaat
     9541 aaacaagtaa ttcaaattct ttcggtgtta gttcaatcag atggtcgtct acggtcactt
     9601 gataattggt caaatcagca gtgatttgtc caatcgatag atattcaggc tcagcttcat
     9661 cttttgattg cttacgaggt tcaattcgtc tgaatattgc tttcatacgg gctaatactt
     9721 cgcgaggact aaatggtttg gtcaaatagt cgtcagcacc tatttctagg ccaatgatcc
     9781 gatccacttg gtcatcttta gccgtcagca tcaaaatcgg tgtttcattt ttttcttgac
     9841 gcagccgctg agtgatctcc atgccatcca tcgttggaag catcacatct aaaatgatga
     9901 aatcaaatga ttgttctaat gctaattcta atccttcgcc tccatctgtg gcactggtta
     9961 ccttgtagcc ttctttttcc aaattgaacg tcaagagcgt tacaatagaa ggttcatcat
    10021 caacaactag tatttttttc attcaaatct cctttaaata ggtttcattc tgtttatatt
    10081 ttagcataga atcagtacaa agggctattt tacaaaagaa ataaatggta taataggaag
    10141 gcactttgct gaatagaaag gaagagcagt caatgattgg aatcagtgca tgtttaggcg
    10201 gtctggcctg tcgctatgat ggacagaaaa aagaagtacc agaattgatg gaactagttg
    10261 aaactggaga agctgtcatg gtctgtccag aagtgatcgg cggattacca attcctagag
    10321 atcccgctga aatcattggt ggaaatggat tcgatgtgtg gaaagatcaa gcaaaagtat
    10381 ggacgacaag cggcgaagat gtaacggaag cgtacaaaaa tggcgcgatc aaagcctatc
    10441 aaaaattgca ggaaaaacac attgatctag tcatcatgaa ggaaaaaagc ccttcatgcg
    10501 gaacccattc gatttatgac ggaagttttt ccggaataaa aaaaaatggt gccggagtgg
    10561 ctactgctta ttttattcag caaaaaatga ctgtgctctc tgatgaagaa tggctgaaaa
    10621 ctagaggtga gcagaatgga aatcgaaaag accaatcgaa tgaacgcgct ctttgaattt
    10681 tattcgacct tattgacaga aaaacaaatg aactacatgg aattatatta tgcagatgat
    10741 ttttcattag gtgagatcgc ggaagaatac gaagtaagcc gacaagcagt atatgacaat
    10801 atcaagcgga caagcaagat cttggaggat tatgaaaaaa aactccatct tttttctaat
    10861 tatattgttc gagaacaggt attggaaaat atgactgcat atattacaga taagtacccg
    10921 gaagataaaa aactacttga atatgtacag caaattcaag caatcgaaga ataaataaaa
    10981 ggaatgaaga aaatatggca tttgaaagct taactgaacg tctccagcag gcgatgggaa
    11041 aaataagaaa aaaaggaaaa gtatcagaag ctgacgtaaa agaaatgatg cgggaaatcc
    11101 gccttgcctt actagaagcc gatgttaact tgcaagtagt caaagatttt actaagcgcg
    11161 tacgcgaacg tgcggtaggg gcagaagtgc tggaaagttt atcacctgca caacaaatcg
    11221 taaaaatcgt tgatgaagaa ttgacgatca cacttggttc tgaaacagca gagttaaata
    11281 aatcaccaaa aataccaact gttatcatga tggctggtct tcaaggggca ggtaaaacga
    11341 cctttactgg taaattagcc aattatttaa agaaaaatga aaatgctcgt ccgctcctaa
    11401 tcgcagggga cgtatatcga ccagctgcga tcgaccaatt gaaagtatta ggtcaacagc
    11461 tagacgttcc tgtatttgat atgggaactg aagtcagccc ggtagagatc gtccgtcaag
    11521 ggatggagtt agcaaaagaa aagaaaaacg actatgtatt gatcgatacg gcaggacgtc
    11581 tgcatatcga tgaaacattg atggatgagt tgaaacaaat caaagaattg acgcaaccaa
    11641 atgagatttt gcttgttgtc gatgccatga ctggtcaaga tgccgtcaac gtagctgata
    11701 gcttcaacca gcaattaggt attacagggg tcgtcatcac taaacttgat ggggatactc
    11761 gaggcggggc agcactatcg atccgctcag tgacaggcgc accaatcaaa ttcatcggtt
    11821 ctggtgaaaa attgactgac ttagaagtat tccacccaga tcgtatggct agccgtatcc
    11881 ttggtatggg ggatatgctg acattgattg aaaaagcgca gcaagactac gacgagaaaa
    11941 aagcggaaga acttgcacag aaaatgcggg aaaattcatt cgatttcaat gactttattg
    12001 aacagcttga tcaagtgatg ggtatgggcc cactggaaga tctaatcaaa atgatcccag
    12061 gaatgaatca agtccctgga atcgaaaacg tcaaggtaga tccaaaagat gttgaacgca
    12121 aaaaagcgat ggtttattct atgacgccag cagaaagagc aaatcctgat ctgttgaacc
    12181 caagccgtcg tcgccgaatt gctgccggtt ctggaaacag tgttgttgaa gtgaatcgaa
    12241 tgatcaaaca attcaaggaa tcaagaaaaa tgatgcagca aatgtccaaa ggggatatga
    12301 acatcccagg tatggaccaa atgtttggtt caggcgtcaa aggaaaacta ggcaaaatgg
    12361 cgatgaatcg aatgatcaag aaaaataaga agaaaaagaa aaaacggaaa taagatgaaa
    12421 aaaacagctt cggtacagac tgaaaatgtc tactgccgga gcttttctat cagcaatttt
    12481 tattctttac taatacaatt actctaaacg aagaggtggc acagatgaga tgtccgtgga
    12541 gtgaacaaac agaaagtatg cggcaatatc atgatactgt ctggggtgtt cctgaatatg
    12601 atgaccaaaa gctgttccga aaattgatgc ttgatattaa tcaagccgga ttgagctggc
    12661 agacgatttt aaataaaagt gaagcattcg atgaagcata tgatcgattc gatatcaaaa
    12721 aagtcgcaaa ttatggagaa acaaaaatca gtgaactaat ggaaaacaaa ggaattattc
    12781 gtaaccgaag aaaaatcgaa gcagccgtcc aaaacgccaa agcagtcttg aaaattcaag
    12841 aagaaatcgg cagtttttca gagtatcttt ggtcgttttc taatggaaaa atcgttgatt
    12901 cttcttatca ttcacaagaa gaagtcccaa caacaagtcc tctttcagat gaattagcaa
    12961 aagatctaaa gaaaagagga tttaaattta tcggaagcac aacaatctat gcttttttag
    13021 aagctgtcgg gatcatcaat gatcaccttg acagttgttt ccgcaaagaa gaggtcgtac
    13081 gtatcggcag aaagggcgaa taaagcatgc gaaaaatcat attttatggt gctataagtt
    13141 tagatggcta tttggcaacg aaaacggatg atcttcaatg gctttttgac actccaacag
    13201 gtaagcaaac tacttatgaa gctttttatg aaacaatcga tacgaccatc atgggcagaa
    13261 aaacttatca ggaagcaaaa aaatatttgg agaccgaagc aatttatcca gagaaaaaga
    13321 atttcgtttt ttccacccac gcacaattac aattgccaga tgctgaggtc gtaaacgaag
    13381 atcctgttgc ttttctaaaa aatctacaaa tgacagaagg aaaagatatc tgggttgtgg
    13441 gtggcggtcg attgatcaaa ccgctattgg aacatcaaat gattgatgaa tggtatatcc
    13501 agattacccc tgtattatta ggggacggga ttcctttatt ccaaaaagga acatatgaag
    13561 cgcgttttga aatgctagac acgacacgat tcggtgaatt tatcgaattg cattatgtac
    13621 gaaagtagaa gtaaataaaa taaaaaaggc aactatatgc agaaatgtcc tgtacatagt
    13681 tgcctttttt attaccgtta tgctttttac tctgttaatt tgttagaaaa atctagatac
    13741 gcatctaaaa tcgtttggaa gaaacttaat gttccttcaa cgatattacc attttcatca
    13801 attagattcg caacgtttcc gatatatgct tccggttgct gcaaggttgg cacattcaaa
    13861 aagacaagtg attggcgtag atggtggttt gcgccaaagc cgccgatccc acctggtgat
    13921 gcagtaacga ctaaacctgg tttatgatcc caaacactgt gtccataagg tcttgagcct
    13981 acatctaaag catttttcaa tactgctggc acagaacggt tgtattcagg agtaacgaaa
    14041 acgacaccgt ctagtcccct cacttcctca cgaaaacgag tccattcagc aggagcctgt
    14101 tctggagttt cgagatcctc attgtaaaat ggcagatcat cgatttttac aaatacactt
    14161 tcgtaccctt caggaagcaa tttcgcaaaa gcttctgcta cttttttgtt gtaagaatct
    14221 tttcttaaac taccaataaa aaatccaatt ttttttgtca tacgattttt cctccttcaa
    14281 actatctata ataatcttac aaaagataag taactggagc aaataaaaac acttactgac
    14341 aaaagctaca tagaagatag attacttatt tttaacaaaa aagaagtcta aaatcttcta
    14401 ttttctaaaa aaaatcgaat ttaatcaagc tgtaaagaaa aaaagcttta cagggtaaaa
    14461 aaaaactgtt acactacatc ttgtgaataa aacaatggag gtggaaataa ataatggcag
    14521 ttaaaatccg tttaaaacgt atgggttcta aaaagagtcc gttttaccgt atcgtcgtag
    14581 ctgattcacg ttctcctcgt gatggacgtt tcatcgaaac tgtaggtact tacaatcctt
    14641 tgaaagaccc tgcagaagta gttttaaaag aagacttagt tcttgactgg ttatcaaaag
    14701 gtgcacaacc ttctgacact gtacgtaaca tcctttcaaa agaaggcgtt atgcaaaaac
    14761 accacgaagc taaattctca aagaaataag gtgaccggat atgaaagatt taagcgactt
    14821 agtcttaaca atcgttcgtc cgctagtcac ttatcctgat caagtcaagt tagacgtcgt
    14881 agagtcgaaa gacttttacg agtacaatct aacagtggct cctgaagata ttggccgtat
    14941 cattggaaaa caagggcgtg ttgctaaagc aatccgcaca atcgtttacg gtgttcggat
    15001 caatgcaccg aaaaaagtgc gtttgaatat catcgatgat aaggaaaagt aaataaacgt
    15061 gctgtactcg aattttcgag tgcagctttt ttgttttttt tattaattga agaaatgatt
    15121 atttggcaaa acaagcaaga aagcgtatcc ttataggagg agggagtgaa cagccaatga
    15181 gaaagaaaat gttatttatt ccatttgctc tattgttaag cgcttgtagt tcggttactc
    15241 aagaatcaga caatacacat ctatcaagca gtatatctga aactgttacc agtccagaaa
    15301 aaagtagtac caaaacggct tccaccactg aacaaacaat ggctacctca aataatgaga
    15361 aaaagactgc tttagaccag ttgaaagaac aacagccaaa tgttcccatg cctctagatg
    15421 taccagtctc ttcaggctat ctcaatatcg cagcgacaca tacaaagcaa ggttattcta
    15481 ttttatatta cagaacagat cgtcccctcg ggttgaatgc tgacgaattg aatcaagaaa
    15541 cgcctattgc aacctatcta tatcaatatg ggtttgcttc tagtcaagaa acaatccaag
    15601 ttttgcagcc atttgagatc gacacaaatg gtcagcaagt ggacttaggt tctcgaatca
    15661 cagggtatca gcaaggtgca gcaggttcaa gttttcttga atggcaagaa ggaaactggc
    15721 gtattcgaat ccgtgggaat aatatagaag gccaagatcc cttactatta gctaaagaaa
    15781 tcgtagctta tttggaagaa aacagtctac cagcacctga acaatttggt aaaattactg
    15841 ttgatatggg tgatacgacg aatcgagccg tagaagtaag ctggcaagaa ccgaaaaatg
    15901 cctatacgat cactcatcaa gatcctatgt ctgcgttgaa gatggcagtt tctatgaaga
    15961 ggttgtgaaa tcagcgcgct gatctgagcc catcatttaa ctccaagtca caggaagtaa
    16021 tcaaaaatag cacgccgcat ttttgcataa ttttggctta ataccttaaa agaaaactag
    16081 aattgcctct tcacacatgc taaaataaaa cagacaaaaa agaaatgggg atataagttg
    16141 actgaatact tgaatgtagg aaaaatcgta aacacacaag gtatcaaagg cgaagtccgt
    16201 gttatctcaa caacggattt cccagaagaa cgttacaaaa aaggaacggt gctaacacta
    16261 ttccaagacg gaaaagcacc tgtggaatta acagtcaaaa gtcatcgcaa acataaaaat
    16321 tttgacttat tgagttttga aggacatcct tctatcaatg atgtggaaaa atatcgggat
    16381 ggcatattaa aagtgtcaaa agacaactct gtagatctag ctgaaaatga attctattac
    16441 catgagatca tcggtctgca tgtattagaa gatggaactg agttaggtaa agtaaaagaa
    16501 atcttgtcac caggtgccaa tgatgtttgg gtcgttcaac gtccgaaaaa agctgatata
    16561 ttgcttccat atatcgcttc tgtcgtcaaa gaagtcgatt tagaagctgg agtggtccgt
    16621 gtagaaattc cagaaggact gattgacgat gaagattgat gtattgactt tatttccaag
    16681 aatgtttgaa ggtcctatgg gggaatccat catcggtaag gctgtagata aaggattatt
    16741 agatatcaat atctctaact tccgcgatta ttcagataac aaacatcaga cagtagatga
    16801 ttatccatac ggcggtggag caggtatgct gctcaaagtc cagcccatct atgataacat
    16861 ccaagccatt gaaaaagctg caccggatgt caaaaaaaga gtaattcttc ttgacccagc
    16921 tggtaaacga ttcgatcaga aaatggcaga agaattttca caagaagaac acttagtatt
    16981 catttgtggt cattatgagg gatatgatga acgtatccgc acattagtga cagatgaagt
    17041 ttccttagga gattatgtcc ttactggggg agaattaggt gcgatggtca tgatcgatgc
    17101 aacagttcgg cttttgccag atgtgttagg gaatcagact tcagctcaaa cggattctta
    17161 ttcaaccggt ttattggaac atccccaata tactcgcccg gcagaatata aagggatgaa
    17221 agtaccagaa gtattgacaa atggaaatca taaattgatt gaagaatggc aattaaaaga
    17281 atcactaaga aggacctatc aacgtcggcc tgatcttttg gaaacacttg aatggacacc
    17341 tcaaatgacg aaatttttag aagaaatcaa gaaagaagaa caagaaaaaa cagcagatga
    17401 agccaagtga ctttttctaa aatcttcttt acagtaggtt gataatatgt tagtatttta
    17461 tggtgagtgc aaaagcactt aactattacg atattccgct gtgagaaaag ctcataagaa
    17521 tatttggaat aaggagaaga aaaccatgaa tccattaatc gaagaattaa caaaagaaca
    17581 attacgttct gacatcccag cgttccgccc tggtgacact gtgcgtgttc acgcaaaagt
    17641 tgttgaaggt actcgtgaac gtatccagtt atttgaaggt gttgttatca aacgccgcgg
    17701 tgctggaatc agcgaaactt atacagtacg taaagtttct aacggagtgg gcgttgaacg
    17761 tacattccca ttgcatacac cacgtgttgc aaaaatcgaa gtagttcgct acggtaaagt
    17821 acgtcgtgca aaattgtact acctacgtgc attacacgga aaagctgctc gtatcaaaga
    17881 aatccgtcgt taatcattgt tatcagactg agaaagaacc cttggtattc aagggttctt
    17941 ttttcttagc atgtaatttg aacaccctga tccagttttt tctttaatta ttggtagggg
    18001 agaataatgg attagaataa atctacctat aagtagtagt aaaaaaaaga gcactatgat
    18061 aatatctttc ttaagagtaa ccgctttaat aaaaaatcca gaatattcta acataaatgg
    18121 agtattctta gaatcaaaag gagaaagaag atggtttata cttatacgga gcaagaaaag
    18181 atgaaagttt tagacacatt catgaatgat ggaaaagtaa aacaagtacc aagttcaaca
    18241 aaaaagaaat acatcctatt gaaagaagtc ttgaaacgct ttgaccatgg agtgacttat
    18301 acagaaacag aggttaattc gatactgctg aacgtatttt ctagtggcga ttatgtcgaa
    18361 caaagacgtt atctaatcac atttggcttt ttcaaaagaa gttcagacgg tcgggcatat
    18421 cagatgatgg gaattgaaaa ttagaagttt taatttatac tcattgaaaa aagttcaagt
    18481 atgcgatatt ggttctacac cgcatacttg aacttttttt gtcatctgta tctataatac
    18541 aaagccaaga acgttattca aaatatggat agctatgata aaagctaatg agcgattcgc
    18601 agttaaataa gcaatcgtca gtaaaatcgt tccaggagcg tacatgaaaa aatcaattgg
    18661 atgcgttaca tgaataaaga taattctctt ttatttgtac gaataaatac caaaacgtac
    18721 ctcctaaaat tagattttgg acagatgccc tttaaaaaga taacaagatt tcgttcttga
    18781 aacaatatat atgcttttat tccaatgagg ttgtgaaaac agtattttgt cctgagcgtt
    18841 agaaaattga aaatggggaa tagcaactcg tactttacca ttttttaagt gaatacaaga
    18901 tcagctaatt gaacaccgtt tattcgtgat gagggacaca acactgcagt cgacacattt
    18961 ctcttttatg ttcaaagata acaaatattt aaaacaaaga aaaatagtag ataatcccgt
    19021 cctaactctt ttattcttcc ctcatagctt ccaaatttaa gcaatacttc aattaatca
//
LOCUS       DAMEET010000056        17585 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.057, whole genome shotgun sequence.
ACCESSION   DAMEET010000056 DAMEET010000000
VERSION     DAMEET010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.057"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            363..731
                     /locus_tag="PB780_001741"
     CDS             363..731
                     /locus_tag="PB780_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDE0733795.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            911..1852
                     /locus_tag="PB780_001742"
     CDS             911..1852
                     /locus_tag="PB780_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDE0733796.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1921..2652
                     /locus_tag="PB780_001743"
     CDS             1921..2652
                     /locus_tag="PB780_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HDE0733797.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2796..4976
                     /locus_tag="PB780_001744"
     CDS             2796..4976
                     /locus_tag="PB780_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDE0733798.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4996..5478
                     /locus_tag="PB780_001745"
     CDS             4996..5478
                     /locus_tag="PB780_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HDE0733799.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5494..6255
                     /locus_tag="PB780_001746"
     CDS             5494..6255
                     /locus_tag="PB780_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDE0733800.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKVFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6386..7720
                     /locus_tag="PB780_001747"
     CDS             6386..7720
                     /locus_tag="PB780_001747"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDE0733801.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            8027..8512
                     /locus_tag="PB780_001748"
     CDS             8027..8512
                     /locus_tag="PB780_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HDE0733802.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8505..9188
                     /locus_tag="PB780_001749"
     CDS             8505..9188
                     /locus_tag="PB780_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDE0733803.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9314..11959
                     /gene="polA"
                     /locus_tag="PB780_001750"
     CDS             9314..11959
                     /gene="polA"
                     /locus_tag="PB780_001750"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDE0733804.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            12004..12840
                     /gene="mutM"
                     /locus_tag="PB780_001751"
     CDS             12004..12840
                     /gene="mutM"
                     /locus_tag="PB780_001751"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HDE0733805.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12804..13433
                     /locus_tag="PB780_001752"
     CDS             12804..13433
                     /locus_tag="PB780_001752"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDE0733806.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            13485..13817
                     /locus_tag="PB780_001753"
     CDS             13485..13817
                     /locus_tag="PB780_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDE0733807.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            14129..14620
                     /gene="nrdR"
                     /locus_tag="PB780_001754"
     CDS             14129..14620
                     /gene="nrdR"
                     /locus_tag="PB780_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDE0733808.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14643..16016
                     /locus_tag="PB780_001755"
     CDS             14643..16016
                     /locus_tag="PB780_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDE0733809.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            16016..16942
                     /gene="dnaI"
                     /locus_tag="PB780_001756"
     CDS             16016..16942
                     /gene="dnaI"
                     /locus_tag="PB780_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HDE0733810.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            17071..17511
                     /locus_tag="PB780_001757"
     CDS             17071..17511
                     /locus_tag="PB780_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDE0733811.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         5991 a         3067 c         3865 g         4662 t
ORIGIN      
        1 agaaagaggt cgtgagcctg ccgtttcact ctgagtcaca aagagcaatt tcaaaaaata
       61 gcttctcatg tttgttgaat attgtgtctt gtgaccgagg agttgtcagg agaacaccgt
      121 ttaccagagg ttgtgacttg tttttagtca cgacctcttt ttttatttat tccatataaa
      181 tacacattat ttattccgaa aagtgaaagc gcaaataaca aaaacaacca acttatttat
      241 tattaaagaa aagcgtattt aagtaggacg attctgttga gagctttcgt agattttccc
      301 attttcctat ggtaaactga caaagttgag aatgttagaa cagatcgttt aggaggaaac
      361 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
      421 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      481 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      541 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      601 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      661 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      721 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      781 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      841 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      901 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      961 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
     1021 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
     1081 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
     1141 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
     1201 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
     1261 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
     1321 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1381 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1441 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1501 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1561 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1621 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1681 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1741 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1801 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1861 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1921 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1981 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     2041 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     2101 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     2161 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     2221 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     2281 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     2341 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2401 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2461 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2521 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2581 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2641 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2701 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2761 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2821 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2881 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2941 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     3001 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     3061 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     3121 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     3181 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     3241 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     3301 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3361 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3421 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3481 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3541 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3601 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3661 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3721 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3781 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3841 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3901 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3961 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     4021 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     4081 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     4141 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     4201 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     4261 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     4321 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4381 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4441 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4501 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4561 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4621 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4681 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4741 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4801 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4861 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4921 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4981 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     5041 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     5101 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     5161 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     5221 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     5281 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     5341 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5401 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5461 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5521 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5581 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5641 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5701 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5761 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggtt
     5821 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5881 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5941 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     6001 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     6061 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     6121 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     6181 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     6241 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     6301 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6361 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6421 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6481 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6541 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6601 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6661 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6721 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6781 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6841 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6901 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6961 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     7021 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     7081 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     7141 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     7201 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     7261 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     7321 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7381 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7441 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7501 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7561 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7621 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7681 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7741 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7801 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7861 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7921 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7981 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     8041 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     8101 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     8161 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     8221 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     8281 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     8341 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8401 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8461 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8521 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8581 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8641 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8701 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8761 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8821 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8881 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8941 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     9001 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     9061 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     9121 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     9181 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     9241 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     9301 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9361 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9421 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9481 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9541 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9601 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9661 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9721 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9781 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9841 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9901 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9961 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
    10021 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
    10081 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
    10141 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
    10201 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
    10261 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
    10321 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10381 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10441 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10501 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10561 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10621 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10681 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10741 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10801 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10861 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10921 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10981 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    11041 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    11101 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    11161 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    11221 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    11281 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    11341 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11401 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11461 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11521 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11581 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11641 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11701 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11761 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11821 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11881 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11941 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    12001 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    12061 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    12121 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    12181 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    12241 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    12301 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12361 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12421 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12481 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12541 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12601 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12661 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12721 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12781 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12841 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12901 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12961 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    13021 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    13081 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    13141 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    13201 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    13261 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    13321 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13381 aaatacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13441 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13501 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13561 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13621 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13681 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13741 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13801 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13861 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13921 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13981 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    14041 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    14101 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    14161 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    14221 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    14281 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    14341 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14401 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14461 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14521 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14581 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14641 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14701 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14761 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14821 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14881 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14941 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    15001 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    15061 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    15121 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    15181 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    15241 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    15301 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15361 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15421 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15481 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15541 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15601 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15661 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15721 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15781 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15841 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15901 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15961 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    16021 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    16081 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    16141 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    16201 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    16261 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    16321 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16381 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16441 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16501 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16561 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16621 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16681 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16741 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16801 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16861 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16921 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16981 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    17041 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    17101 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    17161 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    17221 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    17281 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    17341 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17401 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17461 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17521 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17581 tatgg
//
LOCUS       DAMEET010000057        17207 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.058, whole genome shotgun sequence.
ACCESSION   DAMEET010000057 DAMEET010000000
VERSION     DAMEET010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.058"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(94..1113)
                     /locus_tag="PB780_001758"
     CDS             complement(94..1113)
                     /locus_tag="PB780_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDE0733812.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(1135..2142)
                     /locus_tag="PB780_001759"
     CDS             complement(1135..2142)
                     /locus_tag="PB780_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDE0733813.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(2135..2800)
                     /gene="pgmB"
                     /locus_tag="PB780_001760"
     CDS             complement(2135..2800)
                     /gene="pgmB"
                     /locus_tag="PB780_001760"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDE0733814.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(2793..5087)
                     /locus_tag="PB780_001761"
     CDS             complement(2793..5087)
                     /locus_tag="PB780_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDE0733815.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5347..7512
                     /locus_tag="PB780_001762"
     CDS             5347..7512
                     /locus_tag="PB780_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDE0733816.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7816..8649
                     /locus_tag="PB780_001763"
     CDS             7816..8649
                     /locus_tag="PB780_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDE0733817.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(8751..10451)
                     /locus_tag="PB780_001764"
     CDS             complement(8751..10451)
                     /locus_tag="PB780_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733818.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            10958..12118
                     /locus_tag="PB780_001765"
     CDS             10958..12118
                     /locus_tag="PB780_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDE0733819.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            12134..12577
                     /locus_tag="PB780_001766"
     CDS             12134..12577
                     /locus_tag="PB780_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDE0733820.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(13060..13590)
                     /locus_tag="PB780_001767"
     CDS             complement(13060..13590)
                     /locus_tag="PB780_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HDE0733821.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            13695..14246
                     /locus_tag="PB780_001768"
     CDS             13695..14246
                     /locus_tag="PB780_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDE0733822.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(14399..14872)
                     /locus_tag="PB780_001769"
     CDS             complement(14399..14872)
                     /locus_tag="PB780_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDE0733823.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            15010..16482
                     /locus_tag="PB780_001770"
     CDS             15010..16482
                     /locus_tag="PB780_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDE0733824.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            16479..17189
                     /locus_tag="PB780_001771"
     CDS             16479..17189
                     /locus_tag="PB780_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HDE0733825.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         5350 a         3094 c         3535 g         5228 t
ORIGIN      
        1 ttacagataa acaatgtgtt agaagtagta tcttctttta cataaaaagc tgaccatagg
       61 ataaatggtc agcgtattta gtgagtcagc tatttagtat gggttttcta aaggaacgac
      121 tgtctctctt ttgatcaagc gatgagggat gaccaaacgt acgccactgc tcaattcttc
      181 atggatcaat tcgcgcagct tttgcatagc gacacgtcct aattcggaga tatcgatatc
      241 gatacttgta aggtaagggt gtgttaatgt tgagaagatc gagttgttga agctaatgac
      301 ggagagtgtc tctggaatga catagccata catttgcgcc aactgcatcg tgcgaagggc
      361 gaagatatcg tcaatgacca ccaatgctgt ggcttcgttt tctttcaacg ttttctcaaa
      421 ggacagataa tcctccggat gctctagtac tacactggat aacgcaggaa gaccagccat
      481 catcattgct ttttgataac cgaaatatcg ttcgaaatac aaactttcat gagtcgtgtt
      541 tgtcacaaac aggatattct tatgtccatt ctggatcaaa aattctgttg cttgtttacc
      601 tagaagctgg ttatcattat ctacataaac gatttcgttc tcatgatgat aaggctgtcc
      661 gatcaaagtg aaagggatgt ggttttggta taaataatca atgacagtat cttgactatc
      721 tgaatacact agaataaaac catctacttg tttttgtaaa tgcatacgct ggacgttctc
      781 taacagcaat tcaaaatttt tagcagtagc gattgctgta gtcatatcat aattccgcgc
      841 ttcttcgttg attgcttcca tgatctcaag ataaaatggg ttaccgatcc gctcttttga
      901 atcaagaggg ggtaagatta cgccgatggc actggaaatg cgttttccta gattgcgggc
      961 agtcatgtta ggaacataac ctagttcatc cataattttt cggactttct tctttgttgt
     1021 ctcggaaata ctaggatgat cgttgatgac acgagagacg gtcgaggtgg caacaccggc
     1081 ttttttcgcc acatctttta cggtaatagc catgataaaa acacctttct cttgttattg
     1141 tgcagagaac cgataaatcg tctgactgat ccgttcttga ccaggatgga ggttgatcga
     1201 tccaaatttg gggaaatgaa cgatatcagg gatttcttgc ggttcgattg ctaatccgta
     1261 gtttgaatgc atctgttttc cattgacaga gaaatcagct tcgaatccag tcgtagaaaa
     1321 taataccata cttttattag aagttgctat agtcatttgg cgaccagaat gcttctcgaa
     1381 taattgtaag gaagttttgg aaagcctagg atgatgcaaa agaaatacat catctagtcc
     1441 atctggataa catgcaagga tctcttgcag tgttggggat tttctaaagt cgattggcag
     1501 tttttctttt tttgtccaat ttccagtagg cagtttttct tgatctaatt caagcacacc
     1561 atctaaaaag accgttaact gatgttcata gatgtctctt gttccatttc ctgaaaggtt
     1621 gaagtaggca tggttggtcg gattgcagat ggtttcaaca aggctactag cagaggttgt
     1681 catctctaat tcgttttttg ttaaacggta agtgatcgta gcagtgattg gtcctggata
     1741 ggtagaaaat tcatctttta agtaaaagac cacacctatt gatgatggag ttttgaaaac
     1801 ttctacttcc caaaattgga aagaccagcc tttcgtgcca ccgtggatat ggtgtgatcc
     1861 gcagtttttc tccaatctat gctcacccca agaagcatcc cggatccttc cggctacagg
     1921 tccgacagtt gctccaaaaa agcttttgtc tgttagaaca tcttcgaaac gatcgtaaga
     1981 caataaaaca ttttctgatt ttccttcttt gtctggtgcg aagatttggt gcaggcgggc
     2041 accgtaattg agaagaacga cttctaagta gtcgttcttc aatgtgattt tctttatttc
     2101 agtctctgga actggtacga cggagatctc tggattatcc atggtcatgc cacttttctt
     2161 tcaagtaatc gatcgtaaga aaagtcgtat cagggacaag cgggatctcc tctccaaggt
     2221 cttcagcacg tcccacgcca attggttgag cgccacttga gaggatggct tggataccgg
     2281 cttgcgcgtc ttcgatcccg atacattctt ctgcttttag tccgacagct tttgctgcaa
     2341 ggaggaagat atctggcgca ggttttccgt ttgttgcttc tgccgggtcg gcaatagcat
     2401 caaagtaagg tgtaagaccc attttttcaa gtaggaatgg cccgttttta cttgcagaag
     2461 caagagcaat cttgattttt tcttctttta aagaatccag taaaggcacg accccgggga
     2521 agacatcttt tggttcgatc gtctggatca tctctaaata ataatcgttt tttcttttcg
     2581 ctaattctgc aaattcttcc tttgaaaaag agtgttcttt tccgccataa gctaaaagca
     2641 aagagagtga gtcttctcgg ctgacgcctt ttagctgttc gttgaatgtg tggtcgatcg
     2701 taatgccgat ttcctgacca agctttcgcc aagcacgata atggaattca gcagtatcgg
     2761 taatgacacc gtctaaatca aacaataccc ctttaaacat ttgcttcctc ctttcttagc
     2821 gttatttcta gacgatccgc taaagaataa gactgttcat agacgtaaat ttctagagga
     2881 gaaccttcta ataactcgaa agtaacttgt tcatctgtga ctgagatgaa caataaacgt
     2941 ccgcgataat tgatatggaa agaataagct ttccaagttt ttggcagaaa tggtgcgaat
     3001 ctcaactgat catgggcagt tttcatttga gcaaatcctt ggacaatcga caaccagcta
     3061 ccagtcatgg aagtgatgtg caagccgtct tctgtgtcgt tattgtaatt atccaaatcc
     3121 aagcgagctg ttcgctggta catttctaca gctttgcttt ccattcctaa ttctgctgct
     3181 aaaatcgaat ggatacttgg cgaaagggaa gactcatgga cagtcattgg ttcatagaaa
     3241 tcaaagtttt tttgcttttc ttcttttgta aaagtatcat tgaagaaata aatcccctgt
     3301 agtacatctg cctgcttgat gaagcaagat ctaaggattt tgtcccaaga ccaatgctga
     3361 ttcagaggaa gatcgcttgt gggaatatcg gatacaggca tgagatcttt atctaagaat
     3421 gtgtcatgct ggacaaagat tcctaattct tcgtcaactg ggaagtacat gttctctgcg
     3481 atatctttcc atttggctag ttcttcttcg ctgatagtga tagcagcttc tttttgataa
     3541 gttaaataat tttctcgcgt gtaacgcaag acccaagctg cgatcgtatt tgtataccaa
     3601 ttgttattga tattgttctc atattcgttt gggccggtca caccgtggat catgtattta
     3661 tttgctcgtt ttgaaaaatg gacacggtct gcccagaagc gtgcgatttc tgctaaaact
     3721 tccagtcctt catttttcaa atattccgta tcgccggtgt aattcgtata attgtagatt
     3781 gcataggcaa tcgcgccatt tcggtgaatc tcctcgaatg tgatttccca ctcgttatgg
     3841 cattcgacac cagtgaaggt gaccatagga taaagcgcgc cttttaatcc ttgttgctga
     3901 gcgttgtgga ttgcttgcgg cagttgatta tgccgatatt tcagcagatt cttagttact
     3961 tcaggtttgg ctaaagcaag ataaagaggg acagcatacg cttctgtatc ccagtaagtg
     4021 gctccgccgt atttttctcc tgtgaatcct ttggggccga tattcaatcg ttcatcttcg
     4081 ccgtagtaag tagagaatag ttggaacaaa ttgaatcgaa taccttgttg tgcttcttcg
     4141 tctccttcga tgaccacatc ggcaagcttc catcgttctg cccaagccgc tgtttgctct
     4201 tcttttgctt ggatataatt gtttgtctgg ttcgttaatt ctgagacagc tttttctgct
     4261 tgctgcgctt ctggaatatc tctactagta agaacgataa tttctttttc cactgaccat
     4321 gtctcacctt gagataattc tgcagacaag caaccatgta atgctaaagt atcttcgtga
     4381 tagtcaggga tgactgattt tccattaacg aaatggcgca ttgctgcagt aacagtaaat
     4441 tgttcaatcc caaagttgtt tggaatagtt ttagtcgtca aatagccgat ttcttctata
     4501 gttccacgat caatctcatt ccaaaaatgt tcatcatagt tgctgtcttc gttttgaaca
     4561 tttccatcca attttgatac gagtttgact gttccgctac cttcaagcat ttctatagtt
     4621 aaatagatat aagctgattc ttttttgaca atgctcagaa aacgttcgaa ggaaatgcgt
     4681 actttattag tagaagtagt gattgtgaat gtacgagata atacaccatt tttcatatct
     4741 aattcccagt aaaagtcttc tggttccaaa gaagccaagt cgatcagctg atcgttgatc
     4801 gaaatatcta tcgcgataaa gttcaaggcg ttgatcactt ttccaaagta ttctggataa
     4861 ccatttttcc accagccgac acgtgtctta tcgggatacc agacacccgc caaataagta
     4921 ccttgatggt ggtcccctga gtaagtttct tcaaagttcc cgcgcatccc catataaccg
     4981 tttccaatac ttgttaaaga ttcttgcagt cgaagatttt ttttatctaa ttgtgtcgtg
     5041 ctgattttcc atggatcaat agcaaatagt cgtttgatct gtttcatttt tctcctccta
     5101 atgagctgtt ttatacgttc ataataaacg caaccgattg cacaagtcaa tagacaaaag
     5161 aaaatatttc atagaaaaaa gcttaaaaaa ataaattttt aggagaaaat tatagaaggg
     5221 aacacacgta ttataggttt tatagaatgg tatagacaga aaaataaaaa ataaagcatt
     5281 tacaaaaaag aaaacgcacg ctataatacg tttaacgaaa ccgattgcgt aaaggagcta
     5341 aacggaatga aaaagatatt tagttttgag ttttggcaaa aatttggaaa ggctttaatg
     5401 gttgtggtag cagttatgcc ggcagctggg ttgatgatca gtatagggaa atcaattccc
     5461 ttgatcaatc ctgattggac gccgcttgtc acaacgggcg gagtaattga aaatattggt
     5521 tgggcaatta ttggtaatct tcatatttta tttgctttag caataggagg aagttgggca
     5581 aaagaaagag caggcggagc atttgcagct gggatctcat ttattttaat caatcggatc
     5641 actggggcta tctttggtgt cacaagtgac atgcttgcga atgaagatgc atttacccac
     5701 actctgttcg gtacgaagat catggttaaa ggatttttta caagtgtatt ggaagcacca
     5761 gctttgaata tgggagtatt cgtagggatc atcgctggat ttgtcggggc aatggcttat
     5821 aataagtact ataattatag aaaattgccg gatgctttgt catttttcaa tggaaagcgc
     5881 tttgttccgt ttgttgttat cttatggtca acaatcgtgg cattgattct tgctgtggtg
     5941 tggccaaacg tacaggctgg aatcaataat tttggtttat ggattgcaga atcacaagaa
     6001 agcgcaccga ttctagcacc gtttttattt ggaacattag aacgtttact attgccattt
     6061 ggattgcatc atatgctgac gattcctatc aactatacac agcttggcgg atcgtatgag
     6121 attttatcag gcgcacaggc ggggacacaa gtatttgggc aagatccgtt atggttggct
     6181 tgggccactg atttagtgaa tcttaaaggt gcaggggata tgtcgcaata tgagtttgtg
     6241 ttgcataact ggacgccggc tcgtttcaaa gtaggacaaa tgattggttc ttcaggtatc
     6301 ttgatgggaa tggcttttgc gatgtatcgt aatgtcgatc ctgataagaa agcaagatat
     6361 aaatcaatgt atttttcagc agcgttggct gtctttctaa caggtgtaac agaaccattg
     6421 gagttcatgt ttatgtttgc tgctgtgcca ttatatgtca tttattcagt gatccaaggg
     6481 gctgcatttg cgatggcaga tatcttgcca ttgcgggtcc attcatttgg gaatatcgaa
     6541 ttgctgactc ggacaccgtt agctatcaaa gcgggtctag gtggagatct tatcaacttc
     6601 gtgttgtgtg tgatcgcttt tggtgttgtt acgtatttct tagcaaattt cttgatcaaa
     6661 aaattcaact ttgctacacc gggacgtaac ggaaattacg acaatgacag tgaagaaacc
     6721 gttgtatcta acagcggaac aggaacagca gatcagcaag tggtccagat cattcatttg
     6781 ctgggaggaa aagaaaatat caaagatgtc gatgcttgta tgactcggtt acgcgtaagt
     6841 gtaaatgatc gagaaaaagt agggactgaa gaagcctgga aacgagcagg ggcgatggga
     6901 ttgatcgtca aagacaatgg agtccaagca gtttatggac caaaagcaga tgtcttgaaa
     6961 tcagatatcg aagatctgct gcaatcagga gcagagattc cagagccaga aatggtttct
     7021 acaggaaatg gacaagcagg tagtaagcaa tacttaggta tagaacaaga actagtttcc
     7081 gcagcttctg gagaagtgat tcctttatct gaagtaaaag atccggtatt ctcacaaaaa
     7141 atgatgggtg acggctttgc tgttattcca acatcaagag aagttgttgc gccgatcgct
     7201 ggaaaagtga caagtatttt ccctagcaaa catgcaattg ggctggaaac gaaagatggt
     7261 atcgaagttt tgatccatat gggaatcgat acagtccaaa tgaatcagcc agcttttgag
     7321 attgctgtca aagaaggcca agaagtagga acaggaacga aattagctga gatggatctt
     7381 gatgtgatcc aaaacgaaga aaaagatcca acgatcatga tcgtatttac aaacgataaa
     7441 gtagaagaag tagtgatcaa gcaattaggt acagtgacag ctggtaaagt gatcggctcc
     7501 attaaaatgt aaggagaggt tgtgaaaaag ctgtcctgca tcaagtcata agaacagcca
     7561 aacaaaaata gctcctccta tttttcgttt ggttgggctt attaccgaga agcagcagtt
     7621 tgaatactgt ttagtggaga ggtcgtgaaa aagctgccct gcaccaagtg ataaaaacag
     7681 ccaaacaaaa aatagctcct ggtccattga gcttattact gaggagttag caggagaaca
     7741 ctatttgctg gagaggttgt gacaaaaatt ttgtcacaac ccttttgttt cgaaatgaag
     7801 acaaggagaa aaccaatgaa attcatgaca ctcaacacgc atagctggct tgagccagaa
     7861 ccagagaaaa aattgcaaga attagcagat aagatcctgt tagaagatta tgaagtgatt
     7921 gctttacaag aaatcaacca attactggaa tctgaagaag tagaacctgc gaagctaatg
     7981 aagttctgtc cggtaaagaa tcaagtacct atccgtaaag ataattttgc ctaccgttta
     8041 gttcagcttt taaaggaaca cggaaaagag tatttttgga gttgggaaat gagccacatc
     8101 ggttatgata aatatgaaga aggaaatgca ctgcttacga aaaaaacatt ggaaagtcag
     8161 gtccttaccg tctcccaatt tcaaaagaaa gaagattatc agacacgcaa gatcttgatt
     8221 gggaaaacaa agatagatga tcaggacatt tttgtctcga gctgccactt ttcttggtgg
     8281 acagataaaa aaagtggctt ttattttgag tggaaaaact tggaaaacta ctttttggaa
     8341 actcgcgtac cacttttttt cctaggtgat ttcaataatc cagtagactc tcaaggttat
     8401 tatactgtaa gagaaagttg ccttcttctt caagattcgt atgttgtcgc gaatgaaaaa
     8461 ggaaaagcag ctactgtgga aaagaaaata gatggttggg aacaaaatac agaaaaattg
     8521 cgtatagatt ttattttcgt ccctgaagga atgcaggtta aaaaatatca acggattttt
     8581 gatgggatcg atagtccaat catcagtgat cactacgggg tggaaatcga attagatgtg
     8641 aacgaataaa aaatataaca tataacataa aaaaactgat taaaataaaa ctctccaaac
     8701 gattataccg tttggagagt tttttgatcg gatgtattta tcccttttgc ctaagcacgt
     8761 aaaagcttgt atctatgata aataaataaa catagcaagg taaaccagcc aatcaaagta
     8821 agagaaagaa tgaaatagaa atagattggc gtttgataat gaagcgtgag ctcgttgatt
     8881 cctttttgct gcttcacgat tggggtcccg atatctgtta cttcataatc agtcaatgtt
     8941 tttccatttt gctgtagcac agtccgatcg tagacgataa cagggatatg aacagttttg
     9001 tccccagtgt ttttccactt gattgtcaaa agaccgcctg gatgtgtttt tataaaagtt
     9061 gtttcgtttt ctagtatttc ttcgttatac cggtcgtatt tattgtcttt tgtctcctta
     9121 tagattggca gatagtcagg tgtggttttt tggaaaacga acaagctggt agacatatct
     9181 ttgctgaaga atgttttcct tgcttcttcc ggcgtatcga atagataagt atgtcgactg
     9241 acaaatgttt cattttccca cttttccaga tgctggtata agttctggat cgtctgtgct
     9301 gaaaaaataa gaatgccaaa tatacttaaa agacggaacc acttttgttc agaccactgg
     9361 ttcagtgaaa gggcaaggta aagtagaagc aatagcgtaa atggcacgaa aaatcgaaat
     9421 ggaaactgga tcaaactaat aaaaggcaca tttttttcga tcaatgtcgt ccatggaatc
     9481 aagctagtag aaagaaaaaa gaaaacaaga cttgtgtaca acacgatttt ttggaataag
     9541 gtgttctttt gttgaaagaa aataaaaagc aaaaaacctg ataatatcaa tataggaaag
     9601 gccttagggt aatggagcca ataactgcca tccagatcta tcgccttttg actcatttct
     9661 ctgttaacaa agggaggaag aatcttattc ccactgtaaa caatagctaa tcctgcccaa
     9721 atatttgctg tcagaacaaa ataaagcaag accgacttaa tcagttgaag cagtgctttt
     9781 cctttttcag tctggttaaa aaagtaataa agataaaaag ggatatacat caaagctaac
     9841 atgatcgaag acagtaaatg gatctgtgtc ataagtccca ctgagacagc gactttgata
     9901 acagggaact ttttatgaag gacgaaatca gcaataggga tcatacataa aggataaaaa
     9961 caagtccccc agctagtgaa tccttggttg atagaccagt attgaataga atacgtactc
    10021 atgaataaaa cggctactgg tacacttacc caatttcgaa cattgacata tctcagtaaa
    10081 atcagcatag aaaatcctga aataagataa agaatgaaat ttgaaagaat ctgataatga
    10141 aaccagctac ctgcaagcaa cacgatcaat ccttgtaaat aagcgaaaaa tgggccgtaa
    10201 acggcattca cgatcctgcc actttgctgg aatccataaa gggaaggaaa ataatggaaa
    10261 tccccatatt taatttgttg cgcggcatca tagaaccgat tgtaatggaa tggggcatca
    10321 gcacccaaaa ttagtttatg tgtgaataaa tgaggcatga gaaatgccaa agcaatgaaa
    10381 aaaattgcta aaaatgggcc atattttttc aattgtcgaa ctcctttgca aactttagat
    10441 aaattcatca tatatcctat gcgagaggta ggcaagaaat tagaaaattc atgaaaatat
    10501 ttatgaattt tacaagaaaa acataagtga aagttctaag gagagaggat gcgacataag
    10561 ttttgttgca tctccttgtt ttgaaacaga atttttctta atactcgaaa caaagtagct
    10621 ttttcatgac ctctcggtaa acggtgttca atcagctgct tctcggtaat aagctcacta
    10681 gcctagaaaa tatgagaagc tattttctga ctagcgctct tattactcaa agcataacgc
    10741 agttttcaca acctcttcct taaacggtgt tcaaaagctt gcatctgcgg taataagccc
    10801 aacccaacaa aaaatatgag tagctatttt tttgggctgt acttattact tgatgcagga
    10861 cagctttttc acaacctcat cggctttcat tttctatttt tttcctgaac ccatataata
    10921 aaagaaagaa caaaaaaact tgcaagggag gacacatgtg aagcagagta agcaaaaaca
    10981 gtctcgtcga cttttgtttc gtttgttttt attaatatgt atcgtcgtag ggttactgat
    11041 cgctttatat ccattttatg tggattcttt aaatagtttt attgatcaaa aacgtatcga
    11101 acaagtacaa aaacggaccg cagctgaaat tgaagtgcag cgaaagaaaa tggaggagaa
    11161 aaaccaacga ttaactgacc aaggattcaa tcctggtact gatcctttca atgaaaagaa
    11221 cagaaatgaa agtacaacat ccagccaatt agaggaatgg ctgatcggaa gtatcaatat
    11281 acctaagatc caattaaata tttcacttta tgatcggctc aatggtagga tccttgaaaa
    11341 tggagcaggc gtcttgcaag gaacctcttt cccgcttggt ggaaattcga cgcattcagt
    11401 gatctctgct cacagcggtt tacctaatcg acggttattt accgaattag atcgtttaga
    11461 aaacggagac acgtttattt tgactgtttt gggggagaaa ttggcttatc aagttaaaaa
    11521 tatacaagtg gtactgccag atgacacgtc tgttctaacg atttcagaag gagaagactt
    11581 agttaccttg cttacatgta cgccgtatat gatcaacacg catcgtttgc tagtgacagg
    11641 acatcggatt ccttataacg aatcggtaaa aaaagaagaa ggaaaaggaa atcaggaaag
    11701 aaccatgcgg caatggttta ttcttgcagg aacgataata gctgtagtca tactgttagt
    11761 gtttatcgga cgactaatct atcagtatag attaagtaaa aaagtgcttg atttttcatt
    11821 tattattagt gattcgtcag ggaaaccggt aaatggtagt tcattcattt tgaaacataa
    11881 gaaaaaaaca cttactagaa atggtgtgcc gttttcagta caaagcgacc atcatgggaa
    11941 agtcaagctg gatcaattgc ctggtggaac ttatcgaatc gtttcagctg atgatcctaa
    12001 agttgctgtt tcttttggta ttagaaaagt aaaacaagag aagatatact tttttgaggg
    12061 gcgaaaactt gtaaaagagc ttcaaaaaaa tggtttttat tttaaaatca atgaataagt
    12121 aaggagagaa aaaatgatcc gcacaacaac aaaattgtct gccaaagaac tggaatctgt
    12181 tctatcgatt tggctgcatg ctaacttgga ttctcatgaa ttcattccat caacttattg
    12241 ggagaatcaa gtagaatctg ttaggaaagc tttaccagat gcagtgattt acttagcaga
    12301 aatagatcaa gaaattattg gttttgctgg ggtgattgat cagtttatcg caggaatctt
    12361 tgtgaaaagg gaataccggc aacaaagaat cggtacgcaa ctaatgaaaa aaataaaaga
    12421 aaccaataaa acactgactt tgacagtcta tcaaaaaaat caatcagcta ttcatttcta
    12481 tctttcacaa ggttttacaa ttcaaacaca gcaaaaagat gaagcagtaa atgaggagga
    12541 acttgtgatg aaatggaatc aaaacgctga tttttgatga acagaataac gaaataatgc
    12601 tcccagccta attttttgct gggagcatta tttcattttg aagtcactaa tttaactgtg
    12661 tgaatatgca gttgttacaa tctttgataa acggtgttca atcagctgac cctcggtatt
    12721 agctcaaaaa acggcaaaat atgaggaact atttttccat ttgtttctct aatactcagg
    12781 tcaaaacgct gatttcacaa cctcttgata aacggtgttc aaacggctgc ttctcggtaa
    12841 taagcccact agcctagaaa atatgaaaag ctattttcta actagcgttc ttattactca
    12901 aagcataacg ctgatttcac aacctcttga taaacggtgt tcaatcagct gaccctcggt
    12961 attagctcaa aaaacggcaa aacatgagga actatttttc cgtttctttc tctaatactc
    13021 aggtcagaac gctgatttca caacctctct ttcacaacct tattttactc gtttataatt
    13081 ttcatcgaag tattttccgc tacggatatc atctggcatt ccatcgtatg gttcttcgct
    13141 aataacatct tcattgtcta tacctgcatc acccatataa gtgatcgtag caaattcagg
    13201 aacaagtaat tttggataag tttcatattt ttcacgagct gcttccattt ctgcgatatg
    13261 ttcgttttgg aaagtaacag tgatttctgg atgttcttca acccatttag ggaaaaagat
    13321 cattccgtga agtttctttt catttggcat aaagtccaat gccacgtctg tccctgttgg
    13381 ctctgtccaa gtgattttga atacgccgtc cgtcaattta acgatatcag cttcttggtc
    13441 ttttacccaa cggcctttga ccattccgcc atggatacgg taatcaacag tatggtcatt
    13501 tttggcatac cattcatatt cccaaccatt gtcatacgta tagataaagt gagttccaat
    13561 aaaatcttcc aattctttaa attctttcat gtttaagcat ctccttataa attaaaacat
    13621 gtaataaact acatgttttc gattactcat ttatgataga atggatttaa agatttgtca
    13681 aggagggaaa actgatggca agaaaaaata cacttcgata tattttgtta gggttattaa
    13741 gtaagaagaa gatgacagga tatgaattga atcaatcgtt caaaaatgaa ataggagaat
    13801 tttggcaagc aaaacatagt cagatttatc ctgaacttgc aaaaatggaa gagcaaggaa
    13861 tcattcagca tcaagtagag atcacaggtg aaaaattgga aaagaaagtt tatgaattaa
    13921 cagaagaagg gaaggaacaa ttggcagagt ggattcatac acccaccaat gaactaccgg
    13981 ttaatcgaga cgaatttgtt ctgaaattgt atttcgtcaa agacgtgaat gatccggcat
    14041 tagtagaaat tattcagcag cagcgtgacc ttcatgaaga aaaacgactt catctattag
    14101 agcgaaaaaa aacgatcttt cctactgaaa aagaaaaaca tgaaaattac ggacactacc
    14161 tgattttagc gcatgcgatc aatcgggaaa cagaatatgc aaattggcta acagatgtat
    14221 tagaagaaag taaccgaaaa aaataaaaaa atatccgaac agtgatggag aattcctttc
    14281 tatatgaaag aaacgcagat caccgttcgg atattcttat gttcaaacag gccttttttc
    14341 aagtatttat gtagcatcta cttattaaaa agcttggtac ttaggagatt agtggcgttt
    14401 agagttcttt taacaactgt tcgtagtcag ctttttcatc gacaggaagg agcttgtaag
    14461 ttaattcgtc tgtctcgttg tcatgaatga tgtacatctt agcattctct tctttgaagt
    14521 taccgatttg taaaatcgta tagcgttcgt cgatcaattc gatatctttg attgcttttg
    14581 aaaaaacttt acggacatgg tgcattttct tttggcggat ttctgccttt tctgcagctt
    14641 ttgacaaagt aaatccgtca tctaaatatt ttttgatcag ttctactttt atgactgtag
    14701 gtaaacgata ttttctagga caattcgcat cagcttcgat cgaacgaatg aatccctttt
    14761 gttcccaata ccttaattga cgtggagaca cacctacgat atcacttaat tcactgatgc
    14821 caacaaccaa ttgatcattt tccaacaaac gtttaaggga agccccaacc atttttgtca
    14881 ctccttttgt cctcttgctt cccttaatta tatccgtatt aacttatgtg tcaagtaggt
    14941 tgacaaatag cgaatttagg actaaagtat tctagtagtt tcagaaaaaa ttcagaaaga
    15001 aggagaaata tgagtaacaa ccaacccgta gatatttacg gaaaaccata taatcggaca
    15061 cttttagttg ttgtcttatt aattggtaca ttttgtaccg ttttgaatca gaccttattg
    15121 acgacagctt ttcctacatt gatgaaagct ttcgatattt cagcgtctga tgtgcaatgg
    15181 ttaacgactg gtttcttgct agtcaacggg atcatgatcc caataacagc atggctgatc
    15241 aataaattca gttcaagaaa tttatatctt tctgccatga cgatcttttt gatcggaacg
    15301 attacttgct ttacagcacc taattttagt acattactaa tcggtcgttt gatccaagct
    15361 tgcggtgtgg gagtatcgat gccattattg caaaatatca tgctatctat tttcccgcca
    15421 gaaaaaagag gatcagcaat ggggatgtca gggatcgtta ttggacttgc tcctgcttta
    15481 ggccctacat tatctggtta tatcattgat aactaccatt ggcgtgactt gtttggtatg
    15541 gtactgccaa tcgttgttct tgtcttggtt ttagcattct tcctaatgaa aagtgttatt
    15601 ccattgtcaa atcctagcat tgatatttta tccgctattt tatcaacgat tggattcggt
    15661 agtttacttt acggattttc tagtgtagga gatgacggct ggggaagctt gaaagtaatt
    15721 ggtttcttag ttgttggtgt gattttcatc gccttgttct catggcgtca attacatttg
    15781 gaacatccat tcttggaact tcgtgtattc aaatcatcta cattcacgat tgcagctgtt
    15841 ttagctggtg taacaaatat ggcgatggta ggggcagaaa tggtcgtacc attgtatatt
    15901 caaaatattc gtggagaatc agcattccat tctggcttga tgttgttgcc gggtgcattg
    15961 atcatggggg ctatgatgcc tgtgacgggt gcgattttcg ataaatacgg cgcgaaacgt
    16021 ttagccatca cagggatgtt cattttgaca gcggcaactg caccatttgc tttcttgaca
    16081 aaaacgactc cagttttata tatcgtgatc ctttatgcga tccgtatgtt tgggatctcc
    16141 atggtcatga tgccggtaac gacttctggt atgaatgcat tgccaaataa cttgatcagt
    16201 catgggacag cggtaaataa tacattccgt caaatcgcaa gctcgattgg tacagctatt
    16261 ttgatcagtg tgttgacgaa tgtgacaaaa gcaaatatcc caagcaaatc tgtattggat
    16321 aatacgccat tagcttataa agatcaggca atcaatgcca cactgtcagg ttaccatgcc
    16381 gcgtttatcg tagcgatcat ctttgggatt cttggcttta ttattgcctt tttattgaag
    16441 aaaaaagaac ctgttctaat gaaagcgggg gataatgcat gattattgga gttttgattg
    16501 tcagcttgct tgcttttgct ttaacgaacg tgttcgctaa aaaaacatgg caaacctttt
    16561 tatctttgat tttcggacta atcttcgtcg cttcactaag tttgatcgtc gctaatttgt
    16621 ccaatcactt tggaatggaa aaagtaacgg aaacaaaaac agaaaaaatt gtttccagtg
    16681 ctgattccca aggagcagat atgcttttgt ataaagcttt agggaatgga aaagaaaaag
    16741 tgtatcttta ccgaacaaat gaaaaacaag aaaaaccaaa agcaactggt acggataacg
    16801 aaacaaataa agtagaaaag acagacggcg atgctgaaaa agtgacgaaa actacttatt
    16861 gggaatacaa aaacgacatg tataaattct ggttcaatat tgctgataac aatcacgaat
    16921 atgacagccg agtgaataca ttcaaaatcc cagagacatg ggtagaatta agtacggacc
    16981 aagcagccaa gttagctgaa ttagtgaaga aacagcaaag tacgatggaa agtgaagcaa
    17041 aagcttatgt tcaagacggt atggtaaaag caatgactga aaatccaaaa atgagcaaag
    17101 cagagcaaga acaacgtacg aaagccttag ctgctgaatt ccaacaacaa gcattcgcaa
    17161 aattagtgaa agaagcaaaa ggcgaataat attttgaaca ccatgta
//
LOCUS       DAMEET010000058        17165 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.059, whole genome shotgun sequence.
ACCESSION   DAMEET010000058 DAMEET010000000
VERSION     DAMEET010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.059"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(37..846)
                     /locus_tag="PB780_001772"
     CDS             complement(37..846)
                     /locus_tag="PB780_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDE0733826.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            1139..2575
                     /locus_tag="PB780_001773"
     CDS             1139..2575
                     /locus_tag="PB780_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDE0733827.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            2745..3941
                     /locus_tag="PB780_001774"
     CDS             2745..3941
                     /locus_tag="PB780_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDE0733828.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(4003..4188)
                     /locus_tag="PB780_001775"
     CDS             complement(4003..4188)
                     /locus_tag="PB780_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HDE0733829.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            4419..7733
                     /gene="dnaE"
                     /locus_tag="PB780_001776"
     CDS             4419..7733
                     /gene="dnaE"
                     /locus_tag="PB780_001776"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDE0733830.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLGPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            7901..8863
                     /gene="pfkA"
                     /locus_tag="PB780_001777"
     CDS             7901..8863
                     /gene="pfkA"
                     /locus_tag="PB780_001777"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDE0733831.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            8937..10721
                     /gene="pyk"
                     /locus_tag="PB780_001778"
     CDS             8937..10721
                     /gene="pyk"
                     /locus_tag="PB780_001778"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDE0733832.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(10756..11658)
                     /locus_tag="PB780_001779"
     CDS             complement(10756..11658)
                     /locus_tag="PB780_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDE0733833.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            11844..12413
                     /locus_tag="PB780_001780"
     CDS             11844..12413
                     /locus_tag="PB780_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDE0733834.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            12471..12650
                     /gene="rpmF"
                     /locus_tag="PB780_001781"
     CDS             12471..12650
                     /gene="rpmF"
                     /locus_tag="PB780_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDE0733835.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            12820..14241
                     /gene="gndA"
                     /locus_tag="PB780_001782"
     CDS             12820..14241
                     /gene="gndA"
                     /locus_tag="PB780_001782"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDE0733836.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDEAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            14434..15120
                     /locus_tag="PB780_001783"
     CDS             14434..15120
                     /locus_tag="PB780_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDE0733837.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            15117..16622
                     /locus_tag="PB780_001784"
     CDS             15117..16622
                     /locus_tag="PB780_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDE0733838.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
BASE COUNT         5550 a         2983 c         3596 g         5036 t
ORIGIN      
        1 tgtcacaacc tcttttttgt ggaatctaat tctctattat agtttaagta gtttttccag
       61 ataaacagct aaaccgtctt cctgattgct caaaggagtg acatcatttg caatgccttt
      121 gagttggtca gtaccgttag ccatagctac tccccaacct gcataatcca acaattccaa
      181 atcattatgc tcgtcaccaa aggcaatgac gtctttttga tcgatagaca ggtagttagc
      241 aatctcttgc acgccttttg ctttttggat tccttttgaa acaatctcta agatggcagt
      301 aggtccaccc catgtccgaa catcgacata actgccaaat tgacgtgtta attcagcaga
      361 tacagctcct gcaaaacgtt tgtctgttcg gacaagcaaa gatgtaggat ttgtcgtcaa
      421 atttttagga gaaagcaaat tcttctctcc tggacgagaa gaagcaaaaa ttttttcatc
      481 aaagaaatca aaactgtcaa taaaaaaagt atcacgattt tcagcagcga taaaatctaa
      541 atttagctgc tttttttgcg ataagatttc aaaagcgatt tcacgattga tcaacgtttc
      601 tctttcacca tcccagtgct gattagggat gtgtactaat gcgccgttaa aattaaccat
      661 cggtgttgtt aattctaatt gttggtaaaa ctgatggctc attcggaatg ggcgtcctgt
      721 agcaatactg acatgatgac cggcattgat tgcttttttt agagtctctt ttgttttaga
      781 tgtaatcatc gaatcttgat tgagggtagt tccgtccaga tcgatggcaa ttaatttttt
      841 attcaataag ggtccttcct tcctcaacta gcatttgttt tagtctagca tattttaggt
      901 aattaacaag agcaatcagc tattcctaac aaaaactaca gttatggcta tataaatagc
      961 taaacataag ctaaacacga acgttaagta ttattttcgc tttttttgtt aaaaaaacgt
     1021 tgaaaagcga atgaaacgtc tatataattt gaaattgttc gatggatttg ccctatttta
     1081 cttatttttt attattatag ttttccatca gaaaaaaatt cagggagtgg aactttttat
     1141 ggaaaagttt ttcggtttaa agaaaaacaa tacaaatgta tcaactgaaa taatggcggg
     1201 agtaacgaca ttctttgcca tgagttatat cttattcgta aatccgacga ttttgtcagc
     1261 atcaggtatg cccttccaag cagtatttct agcgacaatc attgcatcga ttattggtac
     1321 gctggtaatg ggattgttcg caaatgtacc atatgctcag gcacctggta tgggattgaa
     1381 cgcatttttc acttttacgg tcgtttttgg gcttggttat tcttggcaac aagccttggc
     1441 aatggtgttt atttgcggat taatcaatat ttttattact gtcacaaata ttcgaaaaat
     1501 gattattcgg gcgattcccg aaagtcttca acatgcgatt ggtggcggga tcggtatttt
     1561 cgttgcgtat gtcggaatca aaaatgcagg attcttatct ttttcagcag accagtcagc
     1621 tatttcgagc tctgtcgttg aaggcggaaa agcgacaaat gttacgataa atggtggaat
     1681 cgtccctgcg ttagccaact ttgacaacgc tcctatctta ttggctgtga ttggtttggt
     1741 actgacagcc attttagttg taaaaaatgt ccgtggggct attcttatcg gtatcgtagc
     1801 gaccacagtc cttggtattt ttatgggtgt tgttgattta agttccattg attggcaaac
     1861 gaattcgtta ggtaattcat ttaaagaatt aggaacaact ttcggtgcag catttggttc
     1921 tgaaggcatg caatctttat tcagtgattc ttccaagatt cctcaagtat tgatgacgat
     1981 catcgcattc agtttatctg atactttcga tacgatcgga acatttatcg gtacaggacg
     2041 ccgcacaggt attttctcaa aagaagacga gatcgcgttg gaagatggtc gtgggttcaa
     2101 aacaaaaatg gataaagcac tatttgccga tgcgatcgct acttctgtgg gggctatctt
     2161 tggtacatcg aatacaacaa cttatgtcga atctgcagct gggatcgggg ccggtggacg
     2221 tactggtttg acttcagtag tagtggcagt gctattcgca ttaagtagtt tattctctcc
     2281 actgatcgcc attgtaccaa atcaagcaac tgcgccagca ttgattttag ttggagtaat
     2341 gatgatggct tcttttgcag acatcaaatg gctagatatg gaagaagctt taccggcctt
     2401 ctttgcatcg atctttatgg gattgtgcta tagcatttcc tatgggattg cagcaggatt
     2461 tattttctat acaattgtga aagtaatcaa aggaaaatac aatcaagttt ctgttgcttt
     2521 gtggatcgtt gatattttat tcattatcaa cttcatcatt ctagcaacaa tttaactaaa
     2581 gagaagggat tggaacagta tttgttctga tctctttttg ttttacataa aaagaacgtt
     2641 acatataaaa tgacctttgc gggatttttc tctttttact ggcgagatcg ttgcttgttt
     2701 attcgaaaat aggtatgata aaagagacga aagagggatg atagatggag attaattgga
     2761 gaaaaaattt agtgattgcg tggattggct gttttttcac tggtgcaagt attagtttag
     2821 ttatgccatt cataccagtt tatgttgagc aactaggaac accaaaagat caaatcgaat
     2881 tgttttcggg actagcaatc tctgtgactg cttttgcatc tgccgtggtt gcaccaatct
     2941 ggggaaatct agctgatcga aaaggccgaa aactcatgat gatccgtgca gcagcaggga
     3001 tgacattaac aatgggatct ttggcttttg taccaaacgc gtattggctt ttgatcatgc
     3061 ggttttggaa tgggattttg tctggatata ttccaaatgc cacagcgatg attgcttcac
     3121 aggcacctag agaaaagagc ggatgggcgt taggaacatt gtctaccgga gcgattgctg
     3181 gaaatctgat cggaccttca atgggcggtg cactggctca gtggtttggt atggaaaatg
     3241 tattcttgat cacaggggct ttgttgatga tcacaacggt attgaccatt ttcttagtca
     3301 aagaagattt ccatccaatc gagaaaaaag atctgataag caccaaagaa attttttcaa
     3361 aaatggatca tctctctatt ttgatcggtc tcttcattac gaccttgatt ttacagatag
     3421 gaatcactac gatcagccct attttgacct tatatattcg tgaattgagc ggaagtacag
     3481 aaaatatcct ttttgtcagc ggcttgattg tttcgatagc tggtgtttcc gctgtctttt
     3541 cttcaccgaa actaggaaaa ctaggggata agattgggaa tcaaaaagtt ctaattggag
     3601 gactcatttt atcattttgc tgttatttac ctatggcatt cgtcactaca ccgctccaat
     3661 taggtatact tcgctttatt ttaggctttt caactggcgc tttgatgcct tctgtcaata
     3721 cactgatcag taagattacg cctcaagaag gagtcagccg aatctatagc tacaatcaga
     3781 tgtttagcaa ctttggacaa gttttaggtc ccatgctagg atcgacagtc gcgcatgctt
     3841 ataattacag tgctgttttt attgtgacaa gtttgtttgt tctgtcaaat attcttcttt
     3901 ctttattcaa ctttcgtaaa gttcttcgca gcagattata atggtggtga ggagtagaaa
     3961 ataaaaaaga gcttgagaaa tctcaagctc ttggctgttt ttttacgaat gggcagaggt
     4021 ttcttttctt ggcgcaactg caccaatcaa aaggataatg acacccgcaa tgaatgaaat
     4081 gattgttgtc aaagtaaaat catatgttcc gcttgttaat gcagcaccaa tataaccgac
     4141 tacttgacct agaacgacag cccagaataa tgtaacgata tatcgcataa agaacacctc
     4201 ttttctatct taattttacc agattttctt tgaatgtaaa gaaacgagac caaatctcaa
     4261 taaaatttga gttcttgcga aagaactcat tcctacagat tacaagcaag caaatgaagt
     4321 ggaataatca acttcagcgc ctgctatctt aatgaaagta aaaggatcaa accttttaac
     4381 gctagtgaat tttaacgaaa aaaaaggcgg aatgcaagat gtttttccca caactttata
     4441 caattacttc ttattctctt ttacaaagta cgatacgtat tcgagaatat gtacaagaag
     4501 caaaaaaaca aggctacact tcattagcca taacggataa agatgtctta tacggtgcac
     4561 ctgaattcta tcgtatttgt gtagaagaag gagtacagcc aatcattgga ttacatctta
     4621 gttatactta tgaagaaaaa aattatggct tgcttgcgta cgcaaaagat gaaataggtt
     4681 atcagcagct gatgaaactt tccagccgaa aaatgatcca agaaaaattg gaacttgcag
     4741 aaatgacaga aaccgaccat ctagcgtttg ttgttccggc aaatcattct gtttctgagg
     4801 ctttcaataa agaggataac tttttcggtc attggcagtt tttacttgaa aatgttttta
     4861 ctagaaattt ttatatcggc ttgacagaaa acacgcctga tcaattgcct gaatggtatc
     4921 agtatgtgat aaaagaaaat tattcgcttt gtgcattaca tcccatcgct agtttacaac
     4981 aagaagaact ttttgcactt gaagtgatga aacatatcaa agagggaagc caaatacctt
     5041 tagaaaattt atctgcatct ttaccactaa cagcaaatgt ctatcttctt tcagaacaag
     5101 aaatgatcaa acgattcgaa aaagcgggtc tttcccaagc tgtaataggc gcccagcgtt
     5161 tagctgaatc gatttcattt gaattccagt tccatcagaa attacttccg cattatccag
     5221 ttcctaatgg aaaacaagct ggagaatatt taagagagtt atgctattcg caagtggaac
     5281 gccgaatcac tgactataca aaggaatacc aagaacgttt ggactacgag ttatctgtca
     5341 tccatcaaat gggattcgat gattatttct tgatcgtatg ggatgtaatg gcttttgctc
     5401 atagaaacaa aatcgttacc ggtgcaggtc gtggttctgc tgcgggttca ttagtagcct
     5461 atgtgttaga aatcacagat gtggatccat tagaatatga tttgctgttc gaacgctttt
     5521 taaaccccga acgctttacg atgccagata tcgatttgga tattcctgac aatcgacgtg
     5581 aagaagtatt gaattatgtt cgagaaaagt atggacaata tcatatggca caaatcgcaa
     5641 catttggcac aatggcagca aaaatggttc ttagagatgc agctagagtg tttggtttat
     5701 cgcaaagtga agcaaaccgc tggtcaaaag caatacccaa tcaattgaaa atcacattga
     5761 cccaagctta taaaacttcg catcaattag tagaattagt tcaggcaagc gaaaaaaacc
     5821 aacttttatt caaaactgca gtgattttag aaggattgcc aagacatgtt tcgactcacg
     5881 cagcaggtgt agttatcagc gataagaatc tacttgaact agttccgctc caacgcggtt
     5941 ctgatgaagc ttttctgacc cagtatacga tgaatgaggt agaagcaatc ggtcttttga
     6001 aaatggactt tttaggacta cgcaacttat cgattattga ttttactttg aaagctgtta
     6061 agcgagtaga agggtatgag atccgattaa aagagatccc gctaaatgat gcaaaaacgc
     6121 tgctcttgtt tcaacgaggc gaaacctcag gagtatttca atttgaatct gctggtatcc
     6181 ggaacgtttt acgaagatta ggtccagaaa acatcgaaga tattgccgca gtcaatgcat
     6241 tgtatcgacc aggccctatg caaaatatag ataccttcat tgccaggaag aaaggaaaag
     6301 aagccattcg ctacccagat gattcactga tcccgatttt gaaaaacaca tatggagtga
     6361 tcgtctatca ggaacaaatc atgcagatcg cctcgactat ggctggattt acattagggc
     6421 agtcagatat tctgcgacgg gcagtgagta aaaagaaaaa agacgtatta gatgaagaaa
     6481 agtcgcattt tgtatcagga gcattacaac agggatattc ggaaaaaaca gcagcgaccg
     6541 tatatgaata tatcgaaaaa tttgcaaact atggattcaa tcggtcacat gcatttgctt
     6601 attcattcgt gggattccaa atggcgtatc taaaagccca tcatccaggt gctttcttta
     6661 ccagcctgat gaattctgtt cggcataaca catcaaaatt gaaagaatat attgcagaag
     6721 cacgtaaaaa taaactcaaa ctttcctcgc cgtccattaa tcaaagtaat tccagttttg
     6781 agttggtcaa taaggaagaa atcagattcg gactgacagc gataaaaggt attcggcgtg
     6841 attttgtaga agatatatta aacgaaagaa aacaaaatgg cccgttccag tcagtggatc
     6901 aattcctcat acggatcgat aagcgttggc tgaaattaga gttactgcaa tcactgacag
     6961 cagtaggcgt ttttgatgaa ttatctccta acaggaaaca attgatgctt gatctcgaag
     7021 gaaaaatcca aaatatcgtt tacagtggtg gaagtcttga cttgctggga atcatggcat
     7081 taaaagaaga agaagtcgct gactatacac tagaagaacg cttgaagctg gaagaggagt
     7141 acttaggtat ttatttgtca ggtcatcctg cagaagggtt tgatcgtctt cgtttagcta
     7201 aacaagttca gctgattaca gatatcgtcc ctaatcaacc agtcaattta ttagtgatga
     7261 tcaaggaagt tagagaaatt cggacaaaaa aaggagaact gatggccttt gttgaaggaa
     7321 ccgacagtag tggagaaatt tctgtcacag tttttccaac tctttatcgt aaatatcgta
     7381 atttgtttgc cgttgatcga gtgatctttg ttcaagggcg gagcgaaatc agcaaatata
     7441 atcaggaact gcaactaatt ggagaaagct ttgctgatcc taaccagtta gaagcccagt
     7501 atccttcaca aacttgctat ttgcgtatta cagaagaacg ggaagatccc gagataatga
     7561 aacaattaca agtgattttt cagaaacacc caggatatat accagtagtt atgtatcacg
     7621 aaaaaacgca acgaaaagta gttctgtcag atgcttattg ggtggatgac agcaagagta
     7681 taaaggggca acttatttat ctgcttggcg aagataatgt ggttttcaaa taacttacgg
     7741 taaaaaatac tacttgcttt catttttttt cattttttta tccgtaaatc aagacaagtg
     7801 agaaaaataa tgttagaatg ttagtcgtgg ttaacgcaaa aatggttgta aacctaagtc
     7861 atacttagga taaagtattt taatgaggtg aatgtttatt atgaaacgca tcggaatttt
     7921 gaccagtggc ggagacgcac ctggaatgaa tgcagctgtt cgtgccgttg ttcgtaaagg
     7981 tatttacgaa ggactggaag tatacgggat caattatggt tttgccggtt tagttgctgg
     8041 agatattcgt cgtcttgatg tggcagatgt aggtgacaag atccaacgtg gtggtacatt
     8101 cttgtattct gcacgttatc cagaatttgc aactgaagaa ggtcaattaa aaggaatcga
     8161 acaattgaaa aaattcggaa tcgaaggtct agtggttatc ggaggagacg gctcttatca
     8221 tggcgcaatg gctttgacaa aacgcggttt cccagcagta ggtattcctg gaacaatcga
     8281 taatgatatt cctgggactg atttcacaat cggttttgat acagcaatca atacagtact
     8341 agaatcaatt gaccgtattc gtgacacagc gacttctcat gttcgtacat tcgttatcga
     8401 agtaatggga cgcaatgcag gagacatcgc tttatggtca ggtgtggctg gcggagcgga
     8461 tgaaatcatc atcccagaac atgattttga tatggcttca gttgctaaaa aaatccaaga
     8521 aggccgcgat cgcgggaaga aacattgctt gatcatcttg gctgaaggtg taatgggcgg
     8581 aaatgaattc gctgagaaac tttcagaatt tggcgactat catacacgtg tatctatctt
     8641 gggacacgtt gtccgtggtg gagcaccaag cgcacgcgac cgcgtgttgg caagtaaatt
     8701 cggtgcttac gctgtcgaat tgcttcaaga aggaaaaggt ggattatgtg tcggtatcca
     8761 tgacaacgaa atcgttgcgt ctgacatcat tgatacactt gaaaacaaca aacataaacc
     8821 agacttatca ctatatgatc taaataattc actttcattt taagagtgaa agaaaccaag
     8881 ctatttaagc tgtgtacagc tttaaataaa caaaattgaa tgggagagtt ttttaaatga
     8941 aaaaaaccaa aatcgttagt acattaggac cagcaagtaa ctcagtagaa attatttctc
     9001 aattgattga atctggggca aatgtcttcc gctttaactt ttctcatggt gaccatgaag
     9061 aacaattatc acgtatgact atggtccgtg aagcagttaa aaagacagga aaagatgtcg
     9121 gtattctttt agatacaaaa ggagctgaaa tccgtacaac tgttcaaggc acaacagaag
     9181 ctgattttgg tcgtgcagga tacatcaaat ttgaagtagg agatcaaaca cgcatctcta
     9241 tggatcctga acatgtaggt tcaaaagaaa aaatcgcggt tacttaccca ggattatttg
     9301 acgatgttca cgttggcgga cacattttat ttgatgatgg tttgatcgat atgcaaatca
     9361 ttgaaaaaga cgaagcaaac cgtgaattag tcgtagaagt taaaaatgca ggtatgctag
     9421 gatcacgtaa aggggtaaat gcaccaggcg tttctattag tttgccaggt attactccaa
     9481 aagatgctga cgatattcgt tttggtttag acaacgatat cgactttatc gctgcaagtt
     9541 ttgtacgtaa agcacaagac gttctagata tccgcgaaat cttagaagaa aaagacatga
     9601 ctcatgttca aatcttccct aaaatcgaat ctcaagaagg tatcgacaac attgacgaaa
     9661 tcatcaaagt ttctgacggt gttatgattg cccgcggaga tatgggtgtt gaaatcccag
     9721 ctgaactagt accaatggtt caaaaacgca tcatcaaaaa atgtaatgct gctggtatcc
     9781 cagttatcac tgctacacaa atgttagaat caatgcaaga aaacccacgt ccaacacgtg
     9841 cggaagcttc tgacgttgct aacgctgtat ttgatggcac agatgcaaca atgctatctg
     9901 gtgaatctgc aaacggggac tacccagttg aagctgtttc aacaatggca cgtatcgacc
     9961 aagaagctga aaatgcatta tcagaattag gtacattcca attgaacgaa ttcgataaaa
    10021 cagatgtaac tgaaacaatc ggtttatctg tagctcgtgc tgcaaaaaat ctaggcgtga
    10081 aaacaatcgt tgctgcaact gaatcaggat acactgcaaa aatgatttca aaatatcgtc
    10141 cagatgcaga tatcttggct gttacttttg acgaacgcac aaaacgtggt ttgatgttaa
    10201 actggggcgt ttacccaaca gttgctgaaa aaccatcaac aactgatgaa atgtttgaac
    10261 tagcagctaa aaaagctgtt gaattaggtt tcgcatcaga aggcgattta atcttgatca
    10321 ctgctggtgt accagttggt gaacgcggaa caactaacgt gatgaaaatt caaatgattg
    10381 gttctaaatt attagaagct caaggcgttg gacaaaaatc tgtcgttgca aatgcagtta
    10441 ttgcttcatc tgctgaagaa gctgtaaaaa aagcaaaaga cggaatggtt ctagttgtac
    10501 catcaactga caaagaatat atgcctgcaa tcgaaaaagc agctgctgtt atcgttgaag
    10561 aaggcggatt gacttctcac gctgcagttg taggaattgc acaagatatt cctgtaatcg
    10621 ttggtgctaa aggcgcaaca acaacaatta cagaaggtga attgattacc gttgattcac
    10681 gtcgcggcat cgtttaccgt ggcgaaacaa cagctatcta atttagctta ataaaaaata
    10741 aaaacagctc ttcttttacg aagagctgtt tttttgtgct gtaacgaatt catggataac
    10801 ttggactact tgttcttgaa gaggcgtgac gatctgctgt gttcgttttg ccaatgaaat
    10861 caagaagcga ggctgaactt catctgaaag agggaggcaa actaaatgat cttcaggatg
    10921 gacagcaatc tctgccaaaa aaccgatacc cacattttct cggaccatac tctttaaaat
    10981 cgtcaagtca ttgctttgat aaacaacttt tggatgagta tgtgtttgtt tagaaagctt
    11041 agtgaatgct gcggggtgga cataatgttc gttcaatagt acaaactgtt catctgctat
    11101 atctttgaaa gagacagtat cctgcttgga aagaggatgt gtaggtggca cgacgatcat
    11161 gaaacgtttg tccaaaagta actctgtttc taaatattga tccacgattg gctgtgttga
    11221 gcctaataca gctaaatcga tcctcccttg tcggatcatc ccgtataagt cccgagagcc
    11281 tccatcggta atctcaattt gattcatcaa ttcatgcttg ataaagtaac ttgacagttt
    11341 aggaaagtat tcattgccga taataggagg catgccaaaa cgaagctttt tcttttgcaa
    11401 ctgagtgatt tcagctcttg cagtagaaat ctcttggaga atggtttgtg catggagaaa
    11461 cagctgcttt ccagaaggcg tgacgattaa ttcgtgatgc gatcgatctc ttgtaataag
    11521 tacggtgtca aactcttttt caagacgttg taatgcatat gtaatagtag gttggctcac
    11581 acggaaataa ttagccactt tcgtaaaatt tttttgtttg acgatttgga catagtattc
    11641 gatatctttc gtgttcatga agatcatccg ctcctttaat ggaataatga ttattgtaaa
    11701 tgattctgag gatgatagca aacagaggct ttctaataaa gatttttctg tttttttatg
    11761 aactgcaagg tgtcaagaaa atttcattga caacatgctt tttgccctgt ataattgctt
    11821 ttgttgctta ggagtgatga aaaatgaaat ggtctttatt ggaattgaga aaataccagg
    11881 agacaccgtt aacttttcat gagacgctcg acttgaaagc cgctctcatg aagcgagaca
    11941 atttaatact tgatgttgcg ccagtggaag tcgaaggttt ggtctcagtt gataagaaag
    12001 gctatatcgt tcattacact gtgcagacga ctttgacagt tccttcaaca cgttcactag
    12061 aacctgttga actgcccatg gatttttctg tagacgaagt attcatgaca gaagaacaat
    12121 tccaaagtag agatgaacaa atgtctgcgg atgagatttt gctaattgaa ggtccattat
    12181 tgaatttagc agagtcgatt gaagacaaca tcttattggc gattccaatg cgggtattga
    12241 caaaagaaga ggaagcagca acagaacttc caaaaggcaa tgattgggaa gttgtttccg
    12301 aagaagaata ccagcgcaga aaaatggaag aagccgagaa taaaattgat cctcgtcttg
    12361 caaaactatc tgaacttttt gattctaaat caaaagacga cgataacaag taagattgga
    12421 atatattgtg tctaatttag atacaagact ttacaaggag gtgtagcaaa atggcagtac
    12481 cagctagaag aacatcaaaa gctaaaaaag caaaacgtcg tacacactac aaattgacaa
    12541 tcaaagggtt aaacgaatgt ccaaactgtg gtgaaatgaa aaaaagccat cacgtatgtg
    12601 caaattgtgg ttactatgat ggtaaggacg taatgtcaaa agaagcttaa tacattttaa
    12661 ttagaaaatg taaaacctca aggctaacca tggagccttg aggtttattt ttttgtagtc
    12721 ggtaaataaa aaatatgttg ttctttagtt tgcaatgtag ccgtgttaag ctataatgta
    12781 agagattagg gagaaatccc gtatgtagga ggagttcata tgtcaaaaca acaaatcggc
    12841 gttgtcggta tggccgttat gggtaaaaat ctagccttga acatagaaag ccgcggctat
    12901 tcagtggctt tattcaatcg tacaggggct aaaacaaccg ctgtagtaga agaacaccca
    12961 gataaaaatt tcaaagcaac atatacaatc gaagaatttg tagaatctat cgagaaacca
    13021 cgtcggatct tgctaatggt caaagcagga ccggcaacag atgcaacgat ccaagagttg
    13081 cttcctcacc ttgataaagg cgatattttg attgacggag gaaatacatt ctttaaagat
    13141 acgatgcgtc gtaatgaaga gttagccaac tctggcatca actttatcgg tacaggcgtt
    13201 tccggtggtg aagaaggagc tttaaaagga ccttcaatca tgcctggtgg acaaaaagaa
    13261 gcctatgcgc tagttgcacc aatcttagag caaatttctg caaaagcaga agacgaagca
    13321 ccatgtgtca catatattgg cccaaatgga gccggacatt atgtgaaaat ggtacacaac
    13381 ggtattgagt atggagatat gcaactgatt gctgaatctt atgacttaat gaagaatatt
    13441 ttaaaccttt cagtagaaga aatggctgat atctttaaag aatggaatca aggcgaatta
    13501 gacagctatt tgatagaaat cactgctgac attctgacac gtaaagacga tgaaggaaca
    13561 ggtaaaccag tagtagatgt gatccttgat gcagctggga ataaaggaac aggtaaatgg
    13621 acaagccaaa gcgcgcttga cttaggtgtg ccacttccat tgatcactga atctgtattt
    13681 gcccgctata tttctgctta taaagaagaa cgcgtacaag caagcaagat tttatctcgt
    13741 acgaacgatt ttgagttcac gggagacaaa aaagaattgg tcgaaaaaat ccgagaagcg
    13801 ttgtatttca gtaaaatcat gagttatgcc caaggatttg ctcaattacg tgtcgcttca
    13861 aaagaatttg attgggactt gccatttggt gaaatcgcaa aaatctggcg cgctggatgt
    13921 atcatccgag ctcgattctt gcaaaaaatt acggatgcct atgacaaaaa tcctgagatc
    13981 gaaaatttat tgctagatga ttacttcgtt gaaatcacga aaaaatatca gcaatcagtc
    14041 agagacgtcg ttgccttggc tgtccaagct ggcgtacctg taccgacttt ctcatcagcc
    14101 atcgcttact tcgattcata cagagcagaa cgtctgcctg caaatataat ccaagcccaa
    14161 cgcgattact ttggcgcaca tacctatgaa cgtgtcgaca aagaaggaat cttccattac
    14221 tcatggtacc atgaagaata atagtctttc ggctgttagg aaatgagaaa ataaaaagag
    14281 gacgaaagag ctgaagtcga ttgttatgag tagacttcag ctctttttta aaattgctat
    14341 ccatgataaa atgactatag atttttaagc atattatcgg tataatgata gagtttttaa
    14401 aaagccagta gaatagaaag gataaaataa taaatgagca atattctaat cattgaagat
    14461 gaaaagaact tggcacgatt tgtcgagcta gagttgaagc atgaagggta taacgcagaa
    14521 gtccactaca acggccgaac tggtttagat gcggcattaa ataatgagtg ggatgccata
    14581 ttgttggatc tgatgctccc agaattgaac ggtttagaag tctgcagacg tgtacgccaa
    14641 gtcaaaaata caccaatcat tatgatgact gcaagagatt ctgtgatcga tcgtgtatct
    14701 ggacttgacc acggagcaga tgactacatc gtcaaaccat ttgctatcga agaattatta
    14761 gcacgtcttc gtgcattgtt gcgtcgaatc gatattgaag gcgataaaaa tgtggctaaa
    14821 caaactacta ttacttatcg cgatttgact atcgaaaaag aaaaccgtgt cgtacgtcgc
    14881 ggaaatgaag taattgaact aacaaaaaga gaatacgagt tgctgcttac tttaatggaa
    14941 aacgtgaacg tggtattagc acgtgacgtt ttactaaata aagtgtgggg atacgaaaca
    15001 gaagttgaaa caaacgtagt ggacgtatat attcgttatc ttcgcaacaa aattgacgtt
    15061 cccggggagg aaagctatat ccaaactgta cgcggaacag gttatgtgat gcgctcgtga
    15121 taaagccgaa aaaatcggaa aaaagagaac tgaaagggcc ctccttgaca atcaagtggg
    15181 cctttgcgag ttctttcttc atattcgttg tgtttacaat tttcgctgtc attacctata
    15241 aatcatctat caatctgatt gtggcaaaag aaagaaacaa tgtagagcgt acgatttcag
    15301 aagtagcttc tcgtttggct aactcggatg aagaactgac attagtaaat acgtatcgta
    15361 atctgacgaa agcgacaaat gacggagatg aggtcgtaga tcatacttca ctggtcgaag
    15421 gaaacttaat gaagatcgat tcctttatag ctgagctagg gcagccatca atggatcttt
    15481 atgtttatga tttggacgag aacttgattt ttaaaaccca tgaaaacagt cgaaacctga
    15541 ttcagactac acgtaaagtt ccaacgatcg ttacactaga aggaaaaact ggctttttat
    15601 ctatccagcc tatatattct aaaggtacaa gagaaaagat cggctatgcg caaagcttct
    15661 atgaattatc ttctttctat gatattcgaa ataagctatt attgactctt attgttttag
    15721 aagttgtttc tttgatctta agtagtattt taggtttctt tttatcttct tatttcttaa
    15781 aaccactcaa ggtactcaga gatacaatgg atactatacg taaagatccg caatcagata
    15841 tccacatgcc tgaaatcgac acaaatgatg aattggcaga tttagctgaa atattcaatg
    15901 agatgcttga ccgtatgcgt ttgtatattg aacaacaaga gcaattcgtg gaagacgtat
    15961 ctcatgagtt aaggacacct gttgcaatta ttgaagggca tcttagtctg ctaaatcgtt
    16021 gggggaaaga tgatcctgag atattagaag aatcattaag cgccagcatg caagaaatta
    16081 gccgtatgaa aagcttagtt caagagatgc tagacttatc aagagcagaa caagtagatg
    16141 ttcattatgc gaatgaaaca actaaaggaa aagaagtcac ctatcaagtt tataataatt
    16201 tcaagatatt atatccagac tatgtgatca ctttagatga tgatttgaca aaagaagtga
    16261 ccctgcaaat ttatcgtaat cactttgaac aattaatcat tatcatattg gataacgcag
    16321 taaagtattc aactacacga aaagaagtcc atatctctat ttcttccact ctgaatgaat
    16381 ttgagatcgc gattcaggac tttggcgaag gaatacccaa agaagacctt aataagatct
    16441 tcaatcgttt ttatcgagtg gataaagcaa gagctagaac gaagggtgga aatggtctgg
    16501 ggttgtctat cgctaaacaa ctggttgaaa actataaagg acgtatctta gcagaaagtg
    16561 tagtagggca agggacaatt tttagaatct ttattcctat cgcaaaggca gaagacaagt
    16621 aaagcatgtg ctttacttgt ttttttatat atccaacaaa gaataaggta caaaatacaa
    16681 gcaagttaga aattcaggga tgtcattata aaaaaaacta gcgaacaatt aaatagaata
    16741 acatccgtta attaaacgta agtagctttt agatgaacga aaaaacaatg aaatgctgta
    16801 atattcgtgt ttaaaaataa taaataaaaa tagggaattt tctattgcaa ttttataaag
    16861 agattgctat attattattt gttctaaatg attcctttaa ggtgtcacta aaaattataa
    16921 aaaatagggt tgacgcactt ttgatgtcat gttataataa cgaagtcgtt tggatgaggt
    16981 agctaataaa gcttgatttt atcgaaaaaa acttcgaaaa agttgttgac aaataacaat
    17041 aaagatgata tgataatcaa gtcgctgcta atgagtgaca aagacgttta gaaaaaataa
    17101 acaaagaagt tgttgacatt ctcttctgaa tctgataaga ttaaagagtt gttgaaacaa
    17161 aaaga
//
LOCUS       DAMEET010000059        17007 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.060, whole genome shotgun sequence.
ACCESSION   DAMEET010000059 DAMEET010000000
VERSION     DAMEET010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.060"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..126)
                     /locus_tag="PB780_001785"
     CDS             complement(<1..126)
                     /locus_tag="PB780_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HDE0733839.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
     gene            complement(230..541)
                     /locus_tag="PB780_001786"
     CDS             complement(230..541)
                     /locus_tag="PB780_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDE0733840.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(543..740)
                     /locus_tag="PB780_001787"
     CDS             complement(543..740)
                     /locus_tag="PB780_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HDE0733841.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1134..2861)
                     /locus_tag="PB780_001788"
     CDS             complement(1134..2861)
                     /locus_tag="PB780_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HDE0733842.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2854..4047)
                     /locus_tag="PB780_001789"
     CDS             complement(2854..4047)
                     /locus_tag="PB780_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HDE0733843.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4234..4878)
                     /locus_tag="PB780_001790"
     CDS             complement(4234..4878)
                     /locus_tag="PB780_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDE0733844.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4972..5106)
                     /locus_tag="PB780_001791"
     CDS             complement(4972..5106)
                     /locus_tag="PB780_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HDE0733845.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5108..7240)
                     /gene="feoB"
                     /locus_tag="PB780_001792"
     CDS             complement(5108..7240)
                     /gene="feoB"
                     /locus_tag="PB780_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDE0733846.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7237..7710)
                     /locus_tag="PB780_001793"
     CDS             complement(7237..7710)
                     /locus_tag="PB780_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDE0733847.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8111..8335
                     /gene="nrdH"
                     /locus_tag="PB780_001794"
     CDS             8111..8335
                     /gene="nrdH"
                     /locus_tag="PB780_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDE0733848.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8494..10653
                     /gene="nrdE"
                     /locus_tag="PB780_001795"
     CDS             8494..10653
                     /gene="nrdE"
                     /locus_tag="PB780_001795"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDE0733849.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10703..11668
                     /gene="nrdF"
                     /locus_tag="PB780_001796"
     CDS             10703..11668
                     /gene="nrdF"
                     /locus_tag="PB780_001796"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDE0733850.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11757..12896)
                     /locus_tag="PB780_001797"
     CDS             complement(11757..12896)
                     /locus_tag="PB780_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDE0733851.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13205..13918)
                     /locus_tag="PB780_001798"
     CDS             complement(13205..13918)
                     /locus_tag="PB780_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDE0733852.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14172..14873)
                     /gene="nagB"
                     /locus_tag="PB780_001799"
     CDS             complement(14172..14873)
                     /gene="nagB"
                     /locus_tag="PB780_001799"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDE0733853.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15107..16639
                     /locus_tag="PB780_001800"
     CDS             15107..16639
                     /locus_tag="PB780_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDE0733854.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16830..>17007)
                     /locus_tag="PB780_001801"
     CDS             complement(16830..>17007)
                     /locus_tag="PB780_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDE0733855.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5277 a         3494 c         2911 g         5325 t
ORIGIN      
        1 tgaaaaaaca cattccacta aatttgatgc cggttttcct tgaggttttg ttccagcacc
       61 tcctttaatt tttttaagct cttgatctga acattgttta gcatttagtc tatttttttc
      121 ttccattacg attcccccta caaactataa atacttttat gtcactgatt caatatacct
      181 atttttctaa tcaaaaaatt ttgagacata tttaatatta taagtatcat taaaattgag
      241 atttatctcc ataatctgct cgatataaac catttattcc agcagttctt ccaatttcac
      301 tcaaatttct tattaattcc tcttgatatt cagtgaatct tattttatta gtataagcag
      361 taaaatatag ataattcacc aatctattta ttaaaggact gggatctaat tcatattttt
      421 gtttttctaa ttttaaatat accttttgta aaacctcaag aatatcagaa tatttaggat
      481 ctttacttat agatatatca ttatataatt catgaacaat ttgcttagca ttttttttca
      541 ttttagcact tccctggaat tgctccacct aaaaaaccac ctatagacat tcctgcaata
      601 caagtagttg ccttggccca atcgaccgta catttatttt tagtgcaata cactccattt
      661 ccataatatt ttccactatg agtggtaccc ccatagataa gttgtgtctc tttaatagac
      721 aaaattttta aatgtttcat aatctaatca cccctaaatt aatgttttta gactattatt
      781 ctaacattca ttaaatttca aaaaacaatt ccttcttgaa acgtagcaaa ataattcctg
      841 aataagaatt taatcacttt taagtatata aactatgtat attctttaat attttttgag
      901 aatgcacttc gtacataata aactttaatc acattagtct ttcataccta cgatcgatct
      961 ctttgactcg cacccataaa ttcataatag tttgtcggcg tctttgaacg tttcgaagat
     1021 gagctacgca ttctcttttt ttatagacaa aaaagataga gacgaacatg gagcttccaa
     1081 atttggatat ttgatccaat tttggaagct ccatttattt cgtgtctgtc tttttatttt
     1141 ttccagcttt cgactaattc ttggttctct ttgatccagt cttttgctgc ttgctgtgga
     1201 tcttttccat tgttgatttc caacataact ttttccatat ctttttctga ccaatggaaa
     1261 ttgtctaata ctttgtaagc ttctggctgg tcttctttca atccttttcg aaccatcgta
     1321 tggatctgct cttcgcttcc cattccgttt tcaggatctt ctaagtattt cagatcatat
     1381 ttggcaaaca tccaatgagg tgtccatcct gtaactacaa ttggttcgtg ttttttgatc
     1441 gcttgaccta gtgcaacggt cattgctcct gaagaagatg tctctacttt ccagtctttc
     1501 aagttgtcat atttttggat cgtgttttca gcagccgtaa ctacaccagc tcctggctcg
     1561 attcctatga ttttcttacc tgcctgatcg gttaactcat cgatcgaatt tacatccatg
     1621 taggctggca ctgctaaacc aacttttgct cctgtcaaat tggcacctag gtcttcgact
     1681 tgatctttat attgtgcata ttgtgaacca tgagttttag gtagccaagc tgaaaccatg
     1741 gcatctgact cgccatttga aacagatttc cacatgattg aattatctaa tggcgtcatc
     1801 gttacatcat agcccatttg tttcaatact tcaccgacta catttgttga agcaacttct
     1861 gtatcccatt caacataaga taagttgata cgttttgttt ctttggcaga tgaaaaactc
     1921 cctataccaa tcgcacctaa gatcactact actacagcac caatgatcag tccttgacgt
     1981 tttttacttt tctgtgttgt tgctcctgca gcttttttat tgtttggctg gttcaaatgc
     2041 tgtgtaaaac ggtcaatgat gattgctaag ataactaatg caaccccgtt gacaaatcca
     2101 ttcccgactt gtgctctttg caatgctgat aatacgccgc gtcctaagcc gggtgcaccg
     2161 atcatagagg caataacgac cattgaaagt gacagcattg ttgtttgatt gatccctgcc
     2221 atgatcgtac ttttagctaa aggcaattct agtttgaata gtttttgacg acttgtgctc
     2281 ccgaatgaat cagaagcttc tactaattct ttaggaactt gtcgaatccc taaattcgta
     2341 aaacgaacgg ttggtggcag agcaaagatg actgaggcaa atacccctgg aaccatcccg
     2401 ataccgaaaa atgcaactgc tggaatcaaa taaacgaatc ctggcattgt ctgcataaag
     2461 tcaaggatcg gtttgataat actttgggct ttactgcttt ttgccatcaa gatccctaat
     2521 ggtaccccaa tgatgatcga gatcacacta gataaaagaa caagtgtgac agtattcatc
     2581 aaatcattcc atagaccttg gttataaata aaccatagac cgattaatgt gaacaacgta
     2641 agtcctggac gtttcttaga aataaagaaa gctgctaccg tcaataacaa gataaacaaa
     2701 ggagctggaa tcactaacag taaatttgta attcccgaca tcactgcctg tccaatcgtt
     2761 tgtaaaaatg aaaataggcc agaaaaagtt gtcgtaaacc attctgtgat attttctacc
     2821 cagtttgcca ctggtaattg atagttcaat agattattca tgttcactca cctcgcttgt
     2881 ttctgctaat gcttcaatca cactacctcg gatgacgaca ccttttaatt ttccatcctc
     2941 tacaactgca agtggagtcg gtgagtcata aataatattg aaaatatcac ttaccaccat
     3001 gtctttccca atcaccgtca cattttcatc taggaagtcg atcaatggac gtttttgctt
     3061 cctagcttct aacgcttgat ctgcagtgat gatccctttc aggtgtctct ttctgtcaac
     3121 agccatcagc atgctgactt cttcattacg catccttgtc aatgcaacgt ttggtccatc
     3181 agattcgata ttcgtcgtca atgcaggtac catgatgttt tgagccgtaa gcactttgga
     3241 acgatcgact tcttccacaa attcacgtac ataatcattt gctggattgg tcaatatttc
     3301 ttcacctgtt ccgatctgca tgatttcgcc gtctttcatc aaggcgattc tgtccccaat
     3361 ccgtaaagct tcattcaaat catgggtaat gaaaataatc gttttttgaa cattggcttg
     3421 caggtccagt aattcgtctt gcatttctcg ccggatcagc ggatcaagag ccgagaacgc
     3481 ttcgtccatc agcaagatct caggatcatt tgctaaagct ctggctaggc caacccgttg
     3541 ttgcatccca ccagataact gactaggata ttggtcttta aatgaaagca aactagaatt
     3601 ttctaatgct ttttctgctt tttcttgtcg ttcttcttta ggtacaccac ggacttctaa
     3661 cccgtattct gtattttcca agatcgttcg atgtggaaac agaccgaagt tttggaaaac
     3721 catattgatt ttatgacgac ggacttctcg caattcttct ttgcttaact tcgatacatc
     3781 ttctccatca atatagatac tgcccgaagt tggctcaatc agacgattca atagacggat
     3841 caatgtggat ttcccactcc cggaaagccc catgatcacg aagatttctc cttcttttac
     3901 gtcaaaattc acgtcataca caccaaccgt tgcaccggtt ttctttaaaa tctccgtttt
     3961 cggctggtga tcattcatca tatctaaagc ggcttgtgtc tttttaccaa atatctttgt
     4021 taaatgtttt acttctactt ttaccaaaca aattctcctt ttttatgtaa attcattttt
     4081 ctttctgaaa aaagtgacca cactatgtta acatcatgta gtcactttgt caaaaatgaa
     4141 agaaatttgc ttgtccaaac tgaaaaaaac ttggctaaat ctttgtaaat aaaaggttta
     4201 gccaagttaa tattttttta atatttcaca caatcattct gcaagctgat gctccgggta
     4261 aaaaaagttt gtcatacgct gtagtgtata ttcatttcca ataaaatgaa cgatgtcccc
     4321 tgccgtaaat ttagcatagg ggccaggtga aacaagcagt tcttcattat gagaaattgc
     4381 gataactgtg gcgccagtcg cctgccagat attcaactcg ctaattgaac ggtccagatg
     4441 cattgctttt tctgataaag ataattcaaa cggaagtaaa ggattcatct tatgtattcg
     4501 cttcgtttga ttgaccagct gatccaataa ttgagaaaaa tgattcagct catcttgttg
     4561 cttctccaca cttgccatga tttctgcttt tgtatcttgg atggattgta catcttgata
     4621 ttgcaatagg aaatcttttg ctttttcctt cgaagcaaca aatgccccac ttccatgccg
     4681 tacttccatg attccaagat ctaccagcac attgatagcc ttgcgggtag tctcaggaga
     4741 agcattgtac gtactggcaa gtgttgagcg ggcatggatc ttctcaccca ctttatatcg
     4801 gttttcaacg atacgttcgg ctaaatcaac agcaatctgc tggtagcgcg ggcggacgat
     4861 ttttttcttt ccagacattt tctttcctct ttcttttttt actttccctc atcttactat
     4921 gctctagcaa aataagcaaa aaaggggaga atagcctctt gcaacgactg atcatcgctt
     4981 gatctgttcc ttttttactg ggcaagctgt ttgacagtca tcacaattct taccggattt
     5041 taaacgagta tagacaattg cgcctgcact tccaaagatt aacacactta ataagaatgt
     5101 cgccatccta ttcctctcct tctaatgaca atatagtata aaactggttc ttggattttg
     5161 gctgacggaa caaaacataa acgagtccta tcatcagtaa aatagcaaga tacgtcccta
     5221 acgctacctg tccaccctcc aataagtgtc ccaactgata aatcacaaaa ctaactaaat
     5281 aagctaaacc gcattgatag cctatggcga tccaagtcca tttcttggca ttcatttccc
     5341 gatggatagc cccaattgcc gcaaaacaag gcgcacaaag taaattgaag gctaacaatg
     5401 aataagctgc tgcgggagta tatgtgtgtt gtagcacttg ccagatttct ctgccatttt
     5461 cggatacttc cgcatgacca aacaaaatac caaatgtgcc aataatattt tctttagcga
     5521 ccaatcctgt aatcgctgcg actgttcctt gccagtttcc ccaaccaagc ggctgaaaaa
     5581 caactgccag gattcgcccg aaaaaggcta agatactttg gtcttcagaa acaggttgag
     5641 cataaaaatt ataagaagag ctgaaccaga tcacgatact agaaacaaag ataatcgttc
     5701 ctgctttttt aacaaaagac ttgctgcggt cataggtttg tcttaggacc gctgaccatt
     5761 gaggaaaatg atatgcgggc aattccatga taaaaggagc tgtatctcca gcaaacaaac
     5821 gtgttttttt caagctgatt ccagagaaaa taattgcagt cataccaaga aaataggcag
     5881 aaggagctac ccaagattga tgaggaaaaa aggcccctgc tactaaggca ataatcggca
     5941 actttgcaga acaaggcatg aaggttgtga ccatgatcgt caaacgacga tcttgctcgt
     6001 tttcaatcgt gcgactggcc atgacgcctg gcactccaca tcctgtagca atcagcatag
     6061 gaatgaagga ttttcctgaa agaccgaatt tcctgaaaag tcgatccatg acaaaagcga
     6121 tcctcgccat atatccgcag tcttccagaa agctcaagca taaaaacaat ataatcaatt
     6181 gcggtagaaa tcctaacact gctcccacgc ctgcgatgat gccgttcaag atcagctcct
     6241 gcatccatcc cgctacttgc cattcagcta acaatctgcc aacatgttct ggtaaccatg
     6301 taccaaacaa tgtgtcattg atccaatctg tccccattgt tcctactgtt tgaatagcca
     6361 gataatagat cagccacatc acaaatgcga agatcggcaa ggctaaccaa cgattcgttg
     6421 caatctgatc gattttgtca ctcatcgata attggaaaga atcatttgac acaacacaga
     6481 gtgtacatag ttgggtaata aattcataac gttcattgac cagaatcgtc tcactatcgt
     6541 cgtccaatag tttctctgtt aactggatca gttgctcgat ctctttttgc tgagaaactg
     6601 ctatctcgta atcctctctt accttctgat ctcgttcaaa atatttcatt gcataccagc
     6661 gtttcttgcc gctttgttct cctagtacat cttcaatttc atgtaaagct gcttctaaac
     6721 gtggatcata tgtacaggga acaggaatag aaggccgctt ttttgcagac tcctgaatct
     6781 tttcgattag tttttcgatt ccttgctttt tcaatacgct gattggtaaa actggtacac
     6841 caagaccata ggaaagcttt tctagattga ttttttttcc ttcttttttg atgacatcca
     6901 tcatattcag accaatgatc ataggaatgt ctaaatccaa taactgcgta gttaaataaa
     6961 gatttcgttc taaattggta gcatcaataa tgttgatgat cagttcaggc tgttcctgaa
     7021 ccaaataatc tcttgcaatc acttcttctg gtgtataagg agacaaagaa tagatgccgg
     7081 gtaggtcctg aatatgaatg ttcggatttt ttttcaacga tccttctttt ttttctactg
     7141 ttacgcccgg ccaattccct acatattgat tcgaaccagt caagagattg aacaagctag
     7201 ttttcccgct gtttggattt ccgactaacg cgatattcat tctttttcct ccctttgtat
     7261 caagattagt tcggcctctg actttcgtaa cgtcagttca taccctcgca ctgtcagttc
     7321 gattggatct cccagagggg ctagttttcg gataactatt tttacatttc gcgtcaatcc
     7381 catatccatt aaccgtcgct tcactgcgcc ttgtccgtgg attgctacga cgatcccagt
     7441 ctctcctgct tttaattgat caagagaagt ccagctttca gaggaagcag tctttttatt
     7501 gacaaagatt tgacctagta tttcccgact tactgccact cgatgacttt ttaccagtaa
     7561 aatggcattg ttgcctgata aattgacgac gacgatttta cttccggcaa cgatcccgat
     7621 attttgcaaa tggcgttttg ttttttcttc tgtttcgatt cgttcgactt gataaacgaa
     7681 attctgcttt gcctgcgcta aggtctgcat attgattcct ctcttgtgaa ataaatcatg
     7741 tattaaaatc attcattata tgttctaatt ttcttacgtg ttacgcaaat aaatgagaat
     7801 cattctcaca tagaatactc tcggtaattg agaatgtcaa ttatacttgt gatattatga
     7861 gcgaatcttt agtattgata gataaaaatg agaaaaaaat atttcttcat agttttccac
     7921 aattttttct ctattaaatt tgctattttc ccacacgata cttgttttca gctctttttt
     7981 caaaaacaca aaacttagta ttccacagtt tccacaacac aatatgtagt gtttgttgtt
     8041 tgactttttt actaagagtt ttatactatc tattatagat aataagaatg tgttagagga
     8101 ggcactcacc atgaacgtaa aaatcttttc taaaaataat tgtatgcaat gtaagatggt
     8161 caaacggttt ttaacagaaa atcaaattgc ttttgaagaa gtcaatatcg atgaacagcc
     8221 tgaagcagta gactggttga aagcacaagg attccaaagt gttcctgtca ttacttctga
     8281 tgcagaaaca gttatcggat tccgtcctga tcagttgcgt aaacttgcca gctaacatga
     8341 ctaaaagaat caaaacgtga tacatggatt tgaagggctt gagacagcac cagtttttgt
     8401 cgcaaacttc caaatccttt tttaaagctt aaaaacaatg caatgatttg caatcaagca
     8461 tcatcaaata cgaaatgaag gttgggcgta cgtatgagtc ttaaaaattt acaagatgtc
     8521 agttatttca agctgaataa tgaaatcaat cggccagtca atggtcaaat ccctttgaat
     8581 aaggataaag aagctttagt tgcctttttt gaggaaaatg tcaaaccaaa tacgatgact
     8641 tttccatcta ttatggaaaa aatcaattac ttgatcgatc aagaatatat agaagcagaa
     8701 ttcattcaat tgtattcacc atcatttatt gaaaagctat atcaattttt ggacgagcaa
     8761 catttttctt tcaaatcttt tatggctgct tacaaatttt attctcaata tgcgttgaaa
     8821 accaacgacg gttccgctta tctggaaact tatgaagatc gagtagcctt caatgcgctt
     8881 tactttgcga acggggatga agaattagca cttgctcttg cagacgaaat gatccatcaa
     8941 cgatatcagc cagctacacc ttctttcttg aatgctggac gtaaacgtcg gggcgaattg
     9001 gtgtcttgtt tcttgactca agtcactgac gatatgaact ctatcgggcg ctcaatcaac
     9061 tctgctttgc aattgtcccg tattggtggc ggcgtaggga ttactttatc aaatcttcgt
     9121 gaagccggcg ctcctatcaa aggatatgag ggtgctgcaa gtggtgtcgt tccagtcatg
     9181 aaattattcg aagatagttt cagctattct aatcaattag gtcagcgtca aggagctggc
     9241 gtggtttatt tgaacgtttt ccatccagac attattgctt tcctttcagc aaaaaaagaa
     9301 aatgcagatg aaaagattcg cgtaaaaact ctttctcttg gcgtgatcgt acctgataaa
     9361 ttttatgaat tgacacgtaa aaacgaagat atgtatttat ttagtccata tagtgtcgaa
     9421 aaagaatatg gtgtcccatt ctcttatgta aatattacag ctgagtatga tcatctagtt
     9481 gcaaatccaa acattcgcaa acaaaaaatc aaagcgcgtg atcttgaaaa tgaaatctca
     9541 aaattacaac aagaatctgg ctatccttac gtaatcaata ttgatacagc gaaccgcgag
     9601 aatccaatcg atggcaagat catcatgagt aatttatgtt cagagatttt acaagttcaa
     9661 acgccttctg tgattaacgg caaacaggaa tatgaagtat taggaacaga tatctcatgt
     9721 aacttaggct cgacaaatat cgtgaatttg atgcaaagcc ctaactttgg tcaatctgtg
     9781 cgtacgatga ctcgtgcatt gacatttatt acagatgcat ctgatatcga tgtcgtacca
     9841 ccgatccaaa acggaaataa gctgaatcat acgattggat taggtgccat gggcttacac
     9901 acttatcttg caaaagaaca aatggaatat ggctcagagg attcattaga ctttacaaat
     9961 atttacttca tgctgttgaa ttattggaca cttgttgaaa gcaacaatat cgctcgtgaa
    10021 agaaaacaag ttttccataa ttttgaaaaa tcagcttatg ctgacggcac ttatttcgaa
    10081 aaatacgtaa ctggagaatt ccagccaaaa tcagaaaaag tcaaagcatt gtttgaaggc
    10141 atttttatcc caactgctga agattggaat gctttgaaac aagcagtaat gaaagatgga
    10201 ctgtatcatc aaaatcgctt ggcagtagca ccaaatggct caatttcata tatcaacgat
    10261 acgagtgcaa gtattcatcc tatcactcgc atgatcgaag aacgccaaga gaagaaaatc
    10321 ggtaaaattt attacccagc gccttatcta tccaatgaga cgattccgta ttacacttct
    10381 gcttatgata tggacatgcg taaagtaatc gatgtttatg caacggcaca acaacacgtc
    10441 gatcaaggca tgagtttgac tttattcatg cgttctgaaa tcccagaagg catttatgaa
    10501 tggaagacaa caacgaagca aacgacacgt gatttgaata ttttaagaca ttacgctttc
    10561 cacaaaggaa tcaaatccat ttattacgta cgtaccttta ctgatgatgc agaagaaatc
    10621 ggaagcaatc aatgtgaaag ctgcgtgatc taatatcata gatagattcg cttattcata
    10681 ttttaattaa aggagcattc tcatgtcaga aacatattat gaagcaataa actggaatga
    10741 aattgaagat atcattgata aatccacttg ggagaagctg acagagcaat tttggctaga
    10801 tacacgtatc ccactttcaa atgacctaga tgattggcgt acattgtctc aattggaaaa
    10861 agatacagta ggacatgtat ttggcggttt gacactgcta gatactgtcc aatctgaaag
    10921 tggcatggat cagttgcgta aagatgtccg cactccccat gaagaagccg ttttgaacaa
    10981 tattcaattc atggaatccg ttcatgccaa aagttattct tctattttta gtacactgaa
    11041 tacgaaaaaa gaaatcgaag aaatttttga ctggacaaat acgaatcctt atctgcaaaa
    11101 aaaagctgag cgtatcaatg aaatctacaa acacggtaca ccgctagaaa agaaaattgc
    11161 cagcgtcttt ttagagacat tcttgttcta ttcaggtttt tatactcctc tttattattt
    11221 aggaaacaac aaattggcta acgtagcaga gatcatcaaa ctaatcatcc gagatgaatc
    11281 tgtacatgga acctacatcg gttacaaatt ccaattagga ttcaacgagt tacctgaagc
    11341 agaacaagaa acgttaaaag actggatgta caatcttctt tatgagcttt acgaaaacga
    11401 agaacgttat acagaagaat tatacgatcc aatcggctgg acagaagaag tcaagacatt
    11461 cctacgctat aatgccaaca aagcattgat gaacctaggg atggatccac tctttccaga
    11521 tactgccaat gatgtcaacc caatcgtaat gaacgggatt tcaacaggca caagcaacca
    11581 tgacttcttc tcacaagtcg gaaacggcta tttgttaggt catgtagaag cgatgaaaga
    11641 cgacgattat ttgattggat tggaataagg atttatttta attcaaaaat ttttcgaaaa
    11701 ataaaaagag tttagcgtac agatatttta aatgtacact gaactctttt ttatgcttat
    11761 tcacttgttt cttccagcac ttttggactg tctaactttt ccgaaacatg ttccattctt
    11821 ctcaataaat agattttccg gatatttaat attatcgtct taatcactgc atagacaggt
    11881 atgcaaatca gcatgcctaa tattcctgcg acacttcctg ctccaatcaa taccaagatg
    11941 atcgtcaaag gatggatatt cattgatttc ccaaataaca acggcttgat caaatttcca
    12001 tctaattgtt ggatcactaa aatcgacaac gccatataca aagcttgaaa tggagagata
    12061 aacaaaccga caatgattgc tggtaccgct ccaataaaag ggccgacata cgggataata
    12121 ttcgtgattc cgcaaatgac cgcaagcaac aagctataag gctgatcgaa tattctcatt
    12181 gccaaaaagc tcattacacc aataatcaaa gcatctaaga tcgtactgct gatataggca
    12241 gataatgttt cgtttaattc ttttaccgtt tggcgtaact ctttacgaat cgctttcgga
    12301 aagaagtgag acaacgcatc aataaatttg tgcccatcct tgaacataaa tagcaaaata
    12361 aatggaacgg taaataacaa aataaaaaat tgcatcataa atgaaatgat ttttgagaca
    12421 cttgcagata aactgactaa tatgatattg aacaaattac taagagataa atttgctgat
    12481 gctaattgtt cttccaaatt gaaattttta aactcttccc gctgcgccaa ttcatttaac
    12541 catttgcttg tttcatctgc atacattggc aagctctggg tcaattgaat tgtctgttcc
    12601 attaactgag gcactacatt catgacagtt aaaacaacta aaatgattac gaccaaaaac
    12661 gtcaataaaa aaccgaacac tctcgggatc cgatgctttt cgagaaaaac aacgattgga
    12721 tcgaacatat aatagataaa accagcaaca agcaatggta aaaaaattgc cgaaagcatt
    12781 ttcaatattg gactaaaaat attcggcatt tgtaaaataa agtacacgcc gatgatcgtg
    12841 agtactagct ccactgtcca gaaaaataat tttgattgtt taaaacgtga ttgcatacat
    12901 ctcctccaag gtcctttgat acttctattc tatcatttaa caaagagtta cgcgctgaga
    12961 ttttcatttg cttaaaaaaa acaaaaaaaa taaacatatt ttaatgagta gacgagtact
    13021 taaacaaaaa cacggtttgg agtagtgaca aaaattatac tgcagtccct ttttccaaat
    13081 aaacggtgtt caaaagcttg catcttcgta ataagcccag ccaaacgaaa aatataagga
    13141 actattttta tttggctggg cttattactt gatgtaggac atctatttta caacctcaga
    13201 tcatttattg cgcaatactg actaaatgca actttgtgat atttttgtgt acggacaact
    13261 cctcgatcac atcggcataa gtgagccctt ttggaagatc aatcgtatag atatttttgt
    13321 acactcggta gtcatcaacg aattttacgt caaaattcac atcttcgatt acgatatttt
    13381 tctcttcaaa ataatcggta agaaaatctt tggtttcttt tctatggata aattggattt
    13441 ctaacttttt agtcgtaggt acatggatca cgcgtttcac tactgtgagt gcaatagcaa
    13501 tcccgataaa actgatgatc gcaatggaat agtaacccat gccgatggcg atccctaatc
    13561 cagcaattgc ccataaagaa gcggcagtcg taagtcccgt caccgattgt ttcgtgacga
    13621 tgatcgttcc cgcgcctaag aaaccgacac cactcaccac ttgagcaatc agccttgctt
    13681 catctgaacg aatcgttcca gcaagctgtg gatactctaa agcatctctt aatgcaccac
    13741 tagcgatctc aacttggatc aatgcaatga tacatgctcc catacaaact aaaatatgcg
    13801 tacgcatacc tgctggacga tttttatatt gtcgttcaaa accgattgct ccgccaaata
    13861 gcatagcgat ccctaaacgt aaaataatct ctggaattgt caataccata tcattcatct
    13921 aaacaccctc cagtccaata tatcatgtct gaaaggagca gacaaaaaaa gtgcaatttt
    13981 tacctcttac gaaaaaagga ctgtgacaaa atttttgtca tagtcctttt tatgaataaa
    14041 cggtgttcaa tcagctgacc ctcggtatta ctcaacaaac ggcaaaatat ggggagctat
    14101 ttttcctttg tcttctctaa tactcaggtc aaaacgctga tttcacaacc tctttagcct
    14161 actattgaaa attacaactt acttgctgct tcttcatcga tgatcacaac aacattgtcg
    14221 tgattttgca atacacttgc tggcaaatga ttcgttacag gtccttcaat catccctttg
    14281 attgcatcag ctttgctttc gccaaatgcc atcaagacga tttctttact tttcataatt
    14341 gaaccgattc ccattgaaac agcttttgtt ggtacatctt ctgctttatc gaagaatcgt
    14401 ttgtttgcat tgattgttga ttctgttaag tcaactactg aagtcgttcc gtcaaatgaa
    14461 gcaccaggtt cattgaaacc gatatgtcca ttttgcccga ttcctaagat ttggatatcg
    14521 attggatgtg catcgatgat ttgatcatac tttttgcatg ctgcttccag atcttctgct
    14581 tttccatctg gtacaaacgt ttctttgaat ggttttttat caaataattg gacatccatg
    14641 aaatgacggt agctttgttc atctgaaccg cctagtccaa cgtactcatc taagtttaca
    14701 gaagtcattt cactgaaatc caaatcgctt gaagtcattt ctttatataa agtaactggt
    14761 gtgcttcctg ttgccaatcc taatactttt gcacctttgt tcataccttc tttgatgatt
    14821 tcaaatgctt tttttccgcc ttcttcagcg ttttttacac gaatgatttc catagtgatt
    14881 gctcctttat attttggtat agcccaatta tagcattctt tttttctttt tacaataatt
    14941 agtatagacc aaattgcatt tttttcattt tcttgacaat aaataggaat ccgtgttagg
    15001 tttcataaga tggttcaatc aaacaatcca tgttgcatga aaaccaagca agtgattcac
    15061 tgtggacgct ttatgtctgc agttttttta tgaaaaagga gttattatga gtatactgac
    15121 attaaacaac attacccagc aatttggcga caaaatcctc tatgaaggtg tcgatctgca
    15181 attaaatgct ggcgaacatc ttgggctgat tggacaaaac ggagccggta aaagtacatt
    15241 gattaaaatc atcactggtg agatcttacc agatgacgga cagatccatt ggcaaaaaaa
    15301 tattcacaaa ggttacctcg atcaatatgt ggaagtcgat gaaacattaa caatcgaaga
    15361 atttctaaaa actgcgtacg caaaagaata cgaaaaggaa gaaaaaattg cttttcttta
    15421 ccaaacatat agcgagacct tcgatgattc cttgctggaa aaagctggga aactccaaac
    15481 ggaattagac caaggagtgt tttatcaaat cgacacctta gttgctgaga tgagcagcgg
    15541 gttggggatc gatgtcttag gtttagattc actgttgaaa aatctaagtg gtggacaacg
    15601 ttcaaaggtg attttagcaa aactactttt agaaaaccca gatgttctga tcttagatga
    15661 accaaccaat cacttagatg atcagcatat ccaatggctg acgcagtttc ttcaggattt
    15721 tgacggtgcg tatatcgtga tttcccatga ccaagaattt cttgatcaga tcaccaccca
    15781 tatcgcagac atcgagtttg gaaagattac taagtatact ggccatctaa aacaagcatt
    15841 gaaacaaaag gaacaaaatc gcgaaagcta tttgcgccaa tatcatgctc aacaaaaaca
    15901 aatcgaaaag accgaagctt atattcgaaa atacaaagcc ggttcccgat caacgatggc
    15961 aaaaagccga caaaaacaat tagataaaat cgaacggctg acgcctccag cttcagcaag
    16021 taaagctgtc tttgattttc cattctcgcc tattgtcacg acattagcag tggaaacaac
    16081 tgatttagtc atcggatatg atcgaccgct acttgcaccc atcaatttga ccatccgctt
    16141 tggtgaaaat attgccattc gaggcttcaa cgggattgga aaatctactt tattaaaaac
    16201 actgatcggt gagattgaaa aaataagtgg cgatttccac ttcccagata acacaaaaat
    16261 caattacttt tcacaagatc tgtactggga aaatccatta gaaacaccac tacaatattt
    16321 aagcagtcaa tttccaaaag cgactatcaa agaattacgt cgccaactag ctaaagcagg
    16381 gttagttaat caattagcaa gtgaacctct cactacatta agtggtgggg aacaaactaa
    16441 ggtgaaactt gcacagttga caatgaacca aggtaattta ctgattctcg atgaaccgac
    16501 gaaccatatc gatcaagaaa cgaaagaaag cttacaagac gggatacaga aatttccagg
    16561 aacagtaatc atcgtctctc atgaacaaga attttatcaa gatcttgtgc aacgagtcat
    16621 tgagattgaa ccaaaataaa cgtttgattg aatagttgaa aaaaaggagc tgtgacaaaa
    16681 gttttgtcac aactccttct tctgaataaa tggtggccaa ttagctgacc ttcggtatta
    16741 gctcaaaaaa cggcaaaata tgaggagcta tttttatttg gctattctta ttacttgatg
    16801 cagaacagct tttcacaacc tcttttcgtc tattttttat tttcgagatt tctctttcct
    16861 ttaaaaacaa ctgtttgaaa gaagaacgca catccttgaa aaacaagccc gctaaccgtt
    16921 acacctaacc agccatactg ctgccacatt gtcgtcccta gcaatgaacc agccgcacct
    16981 ccaataaagt acataaacat aaataca
//
LOCUS       DAMEET010000060        16537 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.061, whole genome shotgun sequence.
ACCESSION   DAMEET010000060 DAMEET010000000
VERSION     DAMEET010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.061"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..196)
                     /locus_tag="PB780_001802"
     CDS             complement(<1..196)
                     /locus_tag="PB780_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733856.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVY"
     gene            complement(381..878)
                     /locus_tag="PB780_001803"
     CDS             complement(381..878)
                     /locus_tag="PB780_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HDE0733857.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1008..1718)
                     /gene="budA"
                     /locus_tag="PB780_001804"
     CDS             complement(1008..1718)
                     /gene="budA"
                     /locus_tag="PB780_001804"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HDE0733858.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1731..3395)
                     /gene="alsS"
                     /locus_tag="PB780_001805"
     CDS             complement(1731..3395)
                     /gene="alsS"
                     /locus_tag="PB780_001805"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HDE0733859.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3600..4262)
                     /locus_tag="PB780_001806"
     CDS             complement(3600..4262)
                     /locus_tag="PB780_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDE0733860.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(4272..5087)
                     /locus_tag="PB780_001807"
     CDS             complement(4272..5087)
                     /locus_tag="PB780_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDE0733861.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(5349..5792)
                     /locus_tag="PB780_001808"
     CDS             complement(5349..5792)
                     /locus_tag="PB780_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HDE0733862.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5926..6264)
                     /locus_tag="PB780_001809"
     CDS             complement(5926..6264)
                     /locus_tag="PB780_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDE0733863.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(6252..6629)
                     /locus_tag="PB780_001810"
     CDS             complement(6252..6629)
                     /locus_tag="PB780_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDE0733864.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6853..8046
                     /locus_tag="PB780_001811"
     CDS             6853..8046
                     /locus_tag="PB780_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDE0733865.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(8212..8634)
                     /locus_tag="PB780_001812"
     CDS             complement(8212..8634)
                     /locus_tag="PB780_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDE0733866.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            9224..9679
                     /locus_tag="PB780_001813"
     CDS             9224..9679
                     /locus_tag="PB780_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733867.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            complement(9841..11013)
                     /locus_tag="PB780_001814"
     CDS             complement(9841..11013)
                     /locus_tag="PB780_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDE0733868.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(11219..12648)
                     /locus_tag="PB780_001815"
                     /pseudo
     CDS             complement(11219..12648)
                     /locus_tag="PB780_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            complement(12645..14147)
                     /locus_tag="PB780_001816"
                     /pseudo
     CDS             complement(12645..14147)
                     /locus_tag="PB780_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345213.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            complement(14144..16471)
                     /locus_tag="PB780_001817"
     CDS             complement(14144..16471)
                     /locus_tag="PB780_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDE0733869.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         4734 a         3301 c         2896 g         5606 t
ORIGIN      
        1 cataaacgtg agtatatttt gccactactt ttccgcattt ttctaatatt tcattgcgat
       61 cgacgtcatg atattcgtct acgcagatca caacacaatt ttctaattta ctgattactt
      121 ctccaaccac tttttgtttc aagccaatag gttggcaaat atattttttt cctaaaatga
      181 tagtttcgtt attcatattt catccatcct taatgttttt ccattattta aaatgataat
      241 accattaaaa tatgattatg aaaatcaata acacttgtga ttttataaaa aaataaaaaa
      301 accgaaaaac agaaatgccc actgtctttc cggctctttt tattcttgat ttctcttctt
      361 ttttgtttca atagacgatt ttagttatta ataaatgaaa aagcatttat attaaaattt
      421 gctaataaaa gatctacata tttttctaga aatgcttgat cgatttcatc ataccctttc
      481 gttactccgc aatcgatatc taaaacaccg attaaattac cttcttttac cattggcact
      541 acgatttctg attgtgcggc tgaatcgcag gaaatgtaat ttgcatgggt tttgacatca
      601 tcaacgatca atgtttcttg tttttcagct gattctccac aaactccttt tcccatcttg
      661 atacgtgtgc aagaaactcg cccttggaac ggtcctaaaa tcaattcatt accatcgaat
      721 aaataatatc ctgcaaagac agtctcaggc aaagtctcat tcaacaatgc agatgaattt
      781 gctaaatttg ctaaccagtt atcttctgct tctaataatc cttgttgctg taataataaa
      841 atttcgtatg catctttttt ctgtttttct gtccacatga taaaaaccgc tcctttttga
      901 tctatggaga agattatagc tcagtttttt tgtaaacgct aggtttcctc ctcaaaaaaa
      961 cgagatacgt caggttaatc accttgaacg catctcgctg tctttttcta ttctgcttgt
     1021 tggatttctg ctgctaaatc agcataatca atcgtttcgt gaacaaaagt cggatcatca
     1081 actggaaaat gctgtacaag ggacgagatg cggctaatct caacttttcc ttcagccact
     1141 tcaaaatcca ttacatgtcc gccaaatgta tgtgtatcat cgataaaatg caagtgaaat
     1201 cccgctgcag caactccttg gaataattca ggcgtaaaga acccgactaa tgttccttgg
     1261 acattgcttt gttggaattc tggttgagac tctgaaattt ttgctagtct tctgtaaggt
     1321 ttttcttgct tcggcatgat acggatgtgc atatttttga ataatccagt tatcttcact
     1381 gccaaaaata ggttttcccc ggcttctctt actaatatgt cttttttcaa ttgttctcca
     1441 tctgcagttt ttgagacaga aaaactagta tctggcgtaa aatctgtaat tgctgcatag
     1501 ggggtaagtt cacttccatc taattgggca actgagccat ctgatcttcc ttgataagct
     1561 acgccatcta aaaagatgac ttcaccatca agcccatgca atgttcccaa tcccagagtt
     1621 cctttttcta atatatgagc aatcgtttct gttccatcca tcaagccagc catcaaagct
     1681 cctaatgtcc cgtgctggta taagattttt tcagtcatgt cgacacttcc ttaatagaat
     1741 tgatctggta agattgtttc tcccaatttc tcattatcac ggtaatcaat cggaatatca
     1801 ataattacgg gtccttctgt cgcaaatcct tcttctaaag ctttttctaa ttcctctaca
     1861 cttgtagctc ggattccttt agcgccaaaa gcttctgcat atttcacaaa atcaactgga
     1921 ccaaagtcta cacctgacgc ccgctgatat ttcatttttt cttggaactc taccatattg
     1981 tagtgtccat cattccaaat caaatgaacg atattcaatt ttttacgtac cgccgtttct
     2041 aaatcctggg cagagaataa aaatccccca tcgccggaaa ctgatacgat ttgtgtattt
     2101 ggacgcacca atgcagcaga aatcgcccat ggcaatgcaa caccaagcgt ttgcatacca
     2161 ttactgaata acaaatgacg aggttcataa cttctaaagt ggcgtgccat ccaaatataa
     2221 tgactaccta catctacggt aacagtcata tcatctgtta cttttgactg aagcgtgtta
     2281 ataacttcta aaggatgcaa gatacctgct tctccttttg attgaacaat gtcccgctcc
     2341 gttaattctg cttgaagaga tgctaaatat tcttttgcat cttctgacac ttgttgaggc
     2401 tctaatgaac ctgtcaataa atcaagagtg gctgagatat caccgatcaa ttctctttca
     2461 ggctgcataa aaggatcgat ttcagctggc gcttcatcga tcacaatgat ccgcgcatct
     2521 ttttccgcat tccagtttct tgcttcatat tcgattggat catagccaat agcaataact
     2581 agatcacttc gtttcaaaag catatctcct ggttggtttc taaataaacc cacacgacca
     2641 aagaaatgcg cttctaattc acgtgagatc actccagctg cttggaatgt ctctacaaca
     2701 ggcaatgctg tctttccaac taattgcctg atggctaacg tttctttttc acttgatcct
     2761 cgcataccta ctaatagtac cggtagtttt gcttctctaa tgcgttcagc taggtaacgg
     2821 atatcatctg cagaagcaga acctaattta ggatcgctca atggcttgat gacattccct
     2881 tggacaggtg catccactac atcttgcggg atactgataa agctcgcgcc tttttttgaa
     2941 ctcttagcca tacgataagc attggcaatc acttctgaaa ttgtttctgg atcttggatt
     3001 tctgcactgt attttgtgat tggctggaac agtgctgcat tatccatgct ttgatgggtc
     3061 aacttcaata gatcgcttcg ttttacttgt ccggcaattg ctaaaactgg gtcaccttct
     3121 gctgtcgctg ttaccaaacc tgtagctaag ttactagcac caggaccact tgttgcgata
     3181 actactcctg gctctcctgt aatacgtccg attgcttgtg ccataaatgc agcattttgt
     3241 tcgtgacgag tcacgattaa ttcaggacct tgatcctcta attcattaaa tacaccatcg
     3301 atttttgcac ctggtatacc gaatacataa tcaacatgat gattaatcaa actttctact
     3361 acagatttcg aaccttgttt cattttttcg tccattgaac atcgtccttt ttatgcttta
     3421 tttttaatta ttagttagaa ataatcacaa ataattgata cagtttctaa acctgtttca
     3481 ttcaaaagta tagcacgttt tatttcattt gtttagccat atgtttcaaa aaatgaaaaa
     3541 gaatgacagt ttttacataa aaactgtcat tcttaattga aagtttcaat ttataaagtc
     3601 tacagatcca tagttctcat caatttacga ttgttgaagt aaatcaaacc aaatacgata
     3661 gaaggaccta gtagaataca ccattggggc tttacgaaaa agaagagaaa caaacttgtc
     3721 aatagtaaaa aatctagtaa actcacatac cattttttca acgtatagta gaacgctatc
     3781 cgtagaactt gatttggttt cgacgctgga tttctcacct gaaaatacca gtagttgatg
     3841 ctaaaggaaa ctgataaaca agcaagtaaa acaaacaacg gaatcaacca ttttccataa
     3901 gaaaaacgta taaaaaagaa acaatcaaaa atcattatca cactaactat ccaagcaaat
     3961 agccaataaa aaaatccctt tagccaaaat tggcgaaacg tctttatgaa caaacgtact
     4021 ggtgaaaaac ttttttcttt tgctaattct tctgtcatac ctatcatcac gatgcaacct
     4081 ggaccaataa aaagtaaaga aaaaataaag gaaaagacat tccttccatc tatcgacaaa
     4141 aggaacgcag ctgctatata caagatattg acacaccaaa aacaaaggct acttgccaat
     4201 gattgataca accaataaaa gaattccata taaacatttt tgtcaaaacg ctgctctttc
     4261 ataatcacct cctattcctt cattcctgat gaagcgatcc cttctacaaa atatttttgc
     4321 atcgctaaaa atataaaagc aacaggaaga acagaaacaa ctgaggcagc catgattaat
     4381 gcgtactctg catcaaatag cccaataaac attttcaacc ctaactggat tgttttattt
     4441 tccgtagaag acaaataaat gaatggcccc atataatcgt tccatgtatt aacgaatgtg
     4501 atgatcgtca aactggcaat cgccggtttt gtaagaggta aaatgatctt gcgatagatt
     4561 ccccactctg ataagccatc gatccgcgca ctctcacata aagaatctgg gatactactg
     4621 tagtattgct tcaacaagaa cacaccaaaa gcattgaacg cttgtaatag caccaacgac
     4681 cacaacgtat atgttaaacc cacttttctc atcataatga actgcgggat catataagac
     4741 tgccaaggga cagcaatcgt gccgatatat gctaaaaata aaatatcgcg ccctttaaaa
     4801 tttagtttag aaaacccgta agcagcaaaa ctagaagtaa acaactgaat caacgtgatc
     4861 acaacgctta aaaaagctgt attcttaaaa aatgtcacta aaggaatacg agaccaaata
     4921 accagataat tctctaaatg ccaagtttta gggatccatt gaatcggaat cgtgaacact
     4981 tcattatttg ctttgaatga agcagaaacc atccataaaa aaggaatgat cgtcaataaa
     5041 gctaatacaa tcagcagaat catacccaac cattgaataa agggcatgtt ttttgaacga
     5101 atttttgctt tttttctttc atttcgaaca cctccaaagt tataatcttc atttctttgt
     5161 aatgattaca atccagaaca acggttatta tagctgattc atttccgttc ttcttatgtt
     5221 tgtaagcgct tatttaacaa gttatgtaaa tctctttatt ttttttgatt taaaaatgaa
     5281 aaaatacaaa agcaagactg tcatacgtct cgcttttgta ttgaaatttc ttcgattaga
     5341 gatacttatt actgttcaaa agcttctttg aaacggtgaa tcaattcatt gactacaggt
     5401 gataaaatcc gattcttttt ccagactagc atgcagttcg tttcaatcat tgggtcaagc
     5461 ggaacgaatg tcactcgctt tgaaagatga cttacagcac cttctatgac taaagcagca
     5521 cctacctgat tctcaattag cgcaaataca ttgaaaatca aattgtacgt tcctgtgatc
     5581 agcaactgct ccttttttat gcctaaccag tcagcaaaca ttcgctgtat ttctggacgc
     5641 tttgagatca aaagaggtac attcgcaaag tcttcttttg ttatcacgtc tttttttgct
     5701 aaaacagaat tttctaaaat gagcaatccc catttttcct ttcttggtaa caaaatcgtt
     5761 tcgtatttct ctgtatctac tggatctagc aaaatcgcta aatctaacag gcttttttct
     5821 agtcactccg tgatatcatc acttgttccg ctgtggatat gaaattgaac gctgggataa
     5881 tcacggatca acttttctag taatagtgcc aatgtattcg aattatcagc ttccacacag
     5941 ccgatcgtaa atcggccgct gaataattgt ttttttctgt tctcgaattc ttgagtggtt
     6001 tgattcgtta aatctaaaat ttcttctgct cggctttttt agaaaataac ctgcttctgt
     6061 caagatcatt ttgcggtttt cgcgtacaaa cagttctgtt cctagttcct cttctaactc
     6121 cttcagttgt cggcttaagg ttggttgtgt aagatgtaga acttctgcgg cttgagagat
     6181 tgttccttct tctgcaattg tccaaaaata ttttagtaag cgtaactcca tagcgatgct
     6241 ccttgatttt tttacttgat acattccatt ttatttctag ataacacacg atcatatttc
     6301 ttgatttttc cttgtaaacg ttccaaacat tcttgcattt cagcaatttt tttcgtcaaa
     6361 agttcacttt cttcaatcaa aagcttacgc cgctcaaata cagttgcttc tccttcgcta
     6421 gataggcgaa tatattcaat caaagactcg atcgataatc cggcatttcg catacagatc
     6481 gtaaagtcga cccattttag gtcttcttct gtataatcac ggattccgcc ttttgtccga
     6541 ttaactggtg gaatcaaccc gattcgttca taatagcgca atgtatcggc cgaaatgccc
     6601 ttttcttcac tgacttgttt gatattcatc tgatcactct ttctcttaat ttcttgccac
     6661 tttacttatg tattttattt attctacaac tttaagtgta ctccaagtca atactcattt
     6721 ctattaaaac taagctggat tatctatatc ggttgtttgt gtattatttc aaataaaaaa
     6781 gtcttgtttt tttattcttt tttggatata cttttaacaa tttagggaaa ttttaggaaa
     6841 aggggtataa aaatgacaac gtatcagcga aagttattat tatcgacatc ggcgggaatt
     6901 gctttagaga atatggatat tatgttttta gctttttcat tatcttcaat gattacttct
     6961 tttcatatta gtggtacgca agcgggtttc atttcaacga tcactaattt ggggatgctc
     7021 attggaggta ttttttttgg catcatagct gataaatatg gacgtgtgaa agtattttct
     7081 caaacggtcc ttctgttttc gattgcttct ttattgatgt attttgcttc aaacatctac
     7141 ttggtttatc tctttcgctt tatcgcaggt gtaggagcgg gaggagaata cggggcgtgt
     7201 atgtccttga tttccgaaag tttttcaaaa aaacaaattg gacgagcttc ttcggttgct
     7261 ggtattggtg cgcaggtcgg tgcggctttg gctgcgattt tagctgctgt gatcattcca
     7321 acgtttggtt ggaagatgtt gtatgtcgta ggtgtactgc cggtgttgat ggtcttgttc
     7381 attcgtcgcg gcttgactga accaaaggaa ttcaaatctg tacaatctgc cgaaaagaaa
     7441 accaaactta gcagactgtt tgaatcaaaa catcttgctt ggcaaacatg tggccttagt
     7501 atcatggtaa ctgtccaaat tgccggctat tttgggttaa tgaattggct gccttcaatt
     7561 atgcaaaatc gattagggct ttcggtttct gggtcttcat tgtggatggt cagtactatt
     7621 ttaggcatgt ctttggggat gatgactttt ggcataatca tggataaatt ggggcctaaa
     7681 ctagcctttt ctttgttttt aatttgttca tccttatctg tctatctact tgtgttagcc
     7741 catagccagt ggagtttggt agcagccgct gtgatagtcg gctactttat caatggcatg
     7801 tatggtggat atggcgcagt gattagtagt ttgtatccaa cagaaatccg cgcgacggcg
     7861 aataatttca ttatgaacct tggacgagct gttggtggtt tttcttctat cgtcatcggt
     7921 ttcttgatgg atcattatag tttaactgcc gtaatcttgt ttttgagcgg tatttaccta
     7981 gtcagtctgc tggtcttgac aactcttact ggtgttacag cattaaggga aagcttgcag
     8041 cattgatagt tgagtctgta aatgacaaga aagaggtgtg gcaaaagctt gcatctgcgg
     8101 taataagacc agccaaatca aaaacatgag acactgtttc cgtttggctg gtcttattac
     8161 ttgatgcagg gcagcttttt caactgctaa tttccttcta tctggcggct actattcttt
     8221 gacgtccgtc atttcaaagt actttactaa taacttagca taacgaaaaa ctgtggatac
     8281 actcgtatat tgagcgtctg cataatcttc caaatttttc tcaggatgtt gatacatgaa
     8341 ttcaatcaaa tgataattat atgattttct gtagaaatga agataaataa tgtagattgg
     8401 gaagttctcc tgcttttcca tagaaaagcg ccgattattc tcattcttca aaatgacctt
     8461 atcaggatat ttttggaaca attcttctaa atctgttaga taattcaaga tagacttact
     8521 tgataccttc aattcgtttt gtagttcttc tattgaacac caacgttctg tttcaagcag
     8581 taaatgaatc attttaattt tcttttgaga aacttttgtt aaaaaagtct gcatattttc
     8641 tcctcctgta tctactttac tatactacat acaagaaaaa aaacggatac aaatatcata
     8701 ttgtcacacc gtcaatagtg cataaacctg ttattatttc tctttttttc ctaagtattc
     8761 tattgatatg aatcatttaa tcgtttatta tataaatgtc aataatctaa taataaaata
     8821 acgtacatat tttattattg catacttaat aacagaacta tagcaaaaaa tcccgtgtat
     8881 caaaacggga ttagattaag tttcctcaat cactattcat aacacaatta ttataacaag
     8941 taagtaaaca attgaaaagg acatttattg aaaacggttg ttttttactt aaggaggaaa
     9001 tttagtgaaa aaattagttg cattgttttt ggtcgcagga agtttgttga gtgggtgtgg
     9061 tgctactacg tctactactc aaagcacaag ttcagctcag gcttccagca gcagtaccac
     9121 gatctccgaa agtacggcag attcatcgaa agaacaagaa caaattgaaa agaaaaaaga
     9181 agaagagaaa caaaaagcgg aaaaagctgc actagaaaaa aaagtgacag aagcggaatc
     9241 tgctttgtca caagctgaag caaatcctac aaaagaaaac tatgcaactg cttcagcagc
     9301 tatccaagcg ctacctgaaa acaaacaaga gttgacaagc cgtcttgctg caattgattc
     9361 cacaatcaaa gcaaaagaag cagaagaaga acggaaaaag caagaagaaa ttgcacgaca
     9421 acaagcagaa caagcgcaac aagaagcaat cgcacagcaa caagcagaac aggcgcgaca
     9481 gcaagcagca gctgaacagg cctctcagca ggcacaagcc gcagctcctg agcaaaataa
     9541 tgaacaaact gtctatgtca caccgaccgg ttcaaaatac catacacata aatgtggaaa
     9601 tggtacgtat acccctgcca ctttaagcga agctcaagct cgtggtttaa ccccttgctc
     9661 caaatgttat ggaaactaat tcaaaagtta tactataata ataaaattct tctgaaacat
     9721 aaaaaaatag aagctggcgt tccagatagt aaaccaaaaa tagcagttta cacgtccatt
     9781 tcgtcagctt ctctcttttt tcatttagtc tgttccctct gcttttttgg acatcttcat
     9841 tcattctttc tcaatcaaat agtacacctc tttttctttt cgcagtttat atcttccttt
     9901 tttcaatttc attgaaaact gtttcgaatg tttatgtttt acatggattt taagcaggag
     9961 ctcctcatgt tgcaagcgat agttcctgcc ttttaaatca gaaaatctca ctagtccgta
    10021 ttcatcagaa gctttcacaa atggaacttt ttctctagta atatagtgtt tgccgttgag
    10081 cgtttttaca gaaaagacca ttccttcgat tggtcgtccg tttgtgttct ttacataaaa
    10141 agataaagga tagtttcgct tagcgatggc caaagaatct aaataagcga tgagtagcca
    10201 aactaagccg attgatccaa gtagtatacc gataaaccaa ataaaaaatt tccatttttt
    10261 ccattgatca attccttgga tcgctttttt tgcctctttt gcttgataag gaatacgatg
    10321 ccccgtcaca agcagtcgat gtgtattgac catataagga gtacaggtaa gcaaagtcac
    10381 gagatctctg cctttttgga tacccaaaga atctacttca tcaggtaaga ccacttggat
    10441 tttttctact tgatacgcca atgtctttcc attgatctgg atgaaaaact ggtctccttt
    10501 tttcaactta ggcaaatctg taaataattt tgcttctggg agtccacgat gcccagaaag
    10561 aacggcatgg gtgtttttcc cgccagtcgg ataagaactt tcttccaata atgacgttcc
    10621 tttttgcaaa aatatttccg ttgtctgatc gaatataggt aagctgacat tgatttttgg
    10681 gatttcgatg actccaagca tatgttccat aaaaaaggca ttactaggta aatcttttaa
    10741 tgcttttgca tcaactgctt ggttgaaact gcctatccct ggtgatacgt tttgctctgc
    10801 caactgttgg tttttctttg tcattttttc ctgctgtttt tttatttcct ctgaattctt
    10861 attcgatgct tgcttttgat aatggatgat catttgctgg gcaagaaaat cattcagtga
    10921 atcttctaca aaaggataag aaaagatccc gatcccaacg actaaaatga ttgccatgaa
    10981 gatgtccagt attcttattc tgcttttatt catcatgtcc acctattttc taatatgtat
    11041 atgaagaggt tgtgaaatca gcgccttgac ctgagtattc gctcaacaaa cggcaaaata
    11101 gttcctccta ttttgccgtt tgttgagcga ataccaagag accgaccact tgtcacaacc
    11161 tttctcccca tttgaatcac tatcgtttat ttatcgtatg atttttgttt ccaatagttt
    11221 aagcttctgt ttcttgatgg cgttttttga agtagatcac tgctcctcca actaaagcag
    11281 tacctaccaa gatgaatacg atgatcccca taccaccagt tgaaggcaag attcctttag
    11341 gttggttatc gatcgttgtt tcatcagaag ttgttggatc atcatctact gtaaattcag
    11401 tgtacgcagc ttcattctta ggtaatgcgt agccttctgg tgcttttgtt tcttgaaggc
    11461 agtatgcccc ttgtttcagc ccttcgattg aaaatttccc ttctgaatca gaaatcaatt
    11521 tcacttcttc ccagtcagaa cctgtgactt catctgacca agcatatggg tattctcctg
    11581 aggcaagatt tgctattttt ttcattaatt taacagttgg atcttttgct cttagaatag
    11641 aaaattctgc atcggctaat ttttcttgcg tgttcacatc ttgtttgatg aagtggaaat
    11701 caccgtagtt ttggacattt tctacagtaa tcgtgttatc cgtagttgta ttttcccgat
    11761 tgactacacc gtttggattc ccaagattgg cttcatctgc gccatggatg ataccggttg
    11821 tttgttgcaa ttttccgttt tttactataa aagtagtttc tttttgtgga ttggttaatt
    11881 gataaccatc tggtgctttt gtttcaacta aagtataatt gccgtctatt aatccattga
    11941 cactgatgga tccttcagta tcagttgtga atgtcaacgc tgactcagca ggatcataga
    12001 gagcgacacc agtgttttca tcaaaaccgg tcacgaattg attctctgaa tttttgatct
    12061 ggaattgcgc ccctgctaac ttatctaccc ctgaaaagtt cccagctttc tggattttga
    12121 tattgctttc tttgatgaca tttttagcat ataggtgaat cgtgtctaaa ggtttgtcag
    12181 ggtctgatga attcgggtta taaaagggta agcttaacac gatatttggc gacgtttcga
    12241 caccgggctt tggtgtttct acaaagacgt atacagcatc tttcccatca gatttacctg
    12301 gaagagtcag gttagctaat ccttcaccag aagtcgttac acttgcgaag ggtgtagcgt
    12361 ctgctggaac tgctgtgtct ttacttggat agtacgtagc agtgatgtca tatacgttga
    12421 attcaacgcc gttcaacggt tcgctattcg gaaatgtcaa ttccgttccg ttgtttattt
    12481 gattttctgg tgctgaagtg aatttcaatt tatgcaaatt aaccgtaaca ttttctggtg
    12541 taggtgatac ggcctagaaa cctctgcagt tcctaggccg agaacgaaag gaagcagcat
    12601 gatagcagtt cctaaaaacg tccatatttt tgttttgatg tttttcattt tatttccctc
    12661 ctattgtttc ctttttgttg ttttttataa aaccaagctg tccctgttga aaggactagc
    12721 aacaacccta aaattgagaa acctccaagc atctcttcgc ttgtttttgg aaagcttcct
    12781 ccattttttt catttgatgg accatttgtc tgcatctgaa ctggcgtttt tccattattc
    12841 gtcggatttg cttttggttc gttgatgatt tccaatggta taggatgaag cggatcataa
    12901 ctgccttttt caacttcaaa gagaatggtt gattgactca atatatatcc tttaggcggt
    12961 ttaatctcca ctagttcgta acttccaaaa gacaatccat ggacagaaaa tgttccatct
    13021 ttactggaag taaaaatcgt tgctcttgaa tcatctttgg ctacccaatg atttatcccg
    13081 ttttcttgcg ctaagtattg gtcatttttt ttgactagaa attgagcatc tgctagtttt
    13141 tgtttcgttt gccgatccac tttgacaaat tgtttgcctc ctgtttggat ggcaagatgt
    13201 gtttctactg tttgattgtc agtcgtcaaa cgtacatcgt tatcaaatgt cgttttttca
    13261 ctgttttctt tcaatgtcat ttggtaacgg atcgttaatt ttttcccagc aaacgaacta
    13321 agatcttttc cagaatctga taagacaaaa ccatgttttg ttttttgtgt gtgaaatgta
    13381 tgaatcttgt ctccaccaat caaaaaatcg atcgtttctg gttctagcca taactgagga
    13441 tcagctgtat ctttgatcgt ataggttcct atctcgttga tgttctcagg aattgttacg
    13501 gtgatgagat aaggaacttt atccccatct gcaaaatctg tctgcttatt ctctactgtt
    13561 tttgttaatg tgagtgcagc aggtattttt tgttcgctct ttggatacaa gtggatggtc
    13621 gtcaatttat gattagatga atctgtcaca ggaagcacaa cgacaaatgg tgctgatttt
    13681 tcttttactt aatctgaagt ctttatttcc gcaaatcggt aaacagcgtc acgaccttgc
    13741 tcatctttgt ccgatgcact aaaagaagca atgccctctt cctcgtctac tgtttttgtc
    13801 acattttctg ccagtatttt ttcactatca cttttttgtg ctagttttct ttgtgcgtct
    13861 tccacactac ttccttctga ccgcagcttg taaaattcat ccgagacatc atatatagcg
    13921 aaagtaacgc cgttcaaccc ttttactcct tgaaagttat ccgggatatt tattccgtcg
    13981 ttttgactga tacttggcat cttatcgctt tcgaacattc gattatgcaa agtaaatgta
    14041 ataggtgatt ctctagctga cacagtcgga gaacctataa aaaagaccag taggataaaa
    14101 aagagaacag caaatatatc cacacgtaca ttttttgttt ttttcatcgc tgttcacctg
    14161 cttttttctt acgatggata aaataaaaga agccagaaaa aataaagatc agtcctaaaa
    14221 agacaaaggg cagtagacct atcccacctg tcgaaggaag caagagagtc ggattgatcg
    14281 tcaacttagc ggtatctgtt gcgataatcg tataattatt gtttacactt ccttcgccta
    14341 aactaatcgt tactatttgt gatccaatat ctgtccggct gttaactgtt agggtataat
    14401 ccaatgattc gcctaagaca acaccggtaa cgtggagatt tccaaccgaa atcgagtgac
    14461 tttgggcatc ataattaaag gcagcgtcat cgacagtaat cactgcgtta gctttataga
    14521 tggtataagt tcctgatgtg ttggctatat cagtaaatga caacttggga tttgctgctt
    14581 tgactttgtc tattccgcta ttcgttagtt taattgtata gctgccagca ttaaccatat
    14641 tttttgctga ttgtgcatct gctgtgtaat ccatactgct aagtgccact tgtactggag
    14701 taagcgaact agtgacaccc tctgctagct tgacatggat tgacgggaca cctgcttttc
    14761 catcatatgt cttactgcca ctgagctcta ccgcaccgct gatttgctta atcgtataga
    14821 catcagatgt ctgcacgata gtaaataaat agtttgttct ggcagctagt ttagctagtc
    14881 cgctttttgt caattgccaa gacgtatagg ttccaatttc agtaggcgca gttacttcaa
    14941 ctcccttttc attcatcgct ttgaaatcag ttggattcca tgtgatttga tcaatactga
    15001 gatcattgtt taaaagggat gttgattctg ttttatcatt aaaggcatat ttaacgatcg
    15061 gtacaacaga aagaccatca tagtacttac tgccatttgc attgattgat actgttgcat
    15121 ttgtcttata atataggatg acttcagaaa tctgttttcc atttgcgtcc tttacgatta
    15181 catttccatt tgtatctaaa ctagctaaaa cactcattgc tgattggttc tttgtttctg
    15241 tcgctgaaac agctgtaaag gtatatccgt tgatagtagg cgtattagtc gtcaggtctt
    15301 gtgcagcact tccgtaagta agattcatta ttttagaagg agcaatcatc tgtccaaaat
    15361 agtcttgata cttaatagtg attggtgttt gtttgcctgt ataggtcacc gtatcgacgt
    15421 tactttctag ggctgtgaaa gaaccgtttg tttgtgcaaa agaatagccc gttactgctg
    15481 ttggagtgat aaccactttg tcacccacat ggattccaga taatgtagtc ccgtacgttc
    15541 cgatttttgc tgctccacca ttgactgtat aagaaaaagt tcctgtccga ttcgcattga
    15601 cctgaaccgt tgctgcttga taggttggaa aatttgaaac atttccctta ccatcaacga
    15661 tctgcccatt tttgatcgag taccccgtga atggaatatc atttgctaac tgtataccga
    15721 ttgaaaaagt actcatacct cctccggcag gaagccctcc ccatgttgag ggtgtaaaag
    15781 aataactttt atccgcattg ggtgtaactg aatcgatcgt atttccagcg gtagccaccc
    15841 ccatatagat actttttggg aatgtgatag tagtaccatt tactgtggag ccaataaaca
    15901 gattatggat atctggacgg ttatcaataa aagcgattgc tttggttacg ccttgtaacg
    15961 cagttttatc cgcactcatc gatccgttaa ccgcaacaat gactccatct ttggcatata
    16021 tcactgtata tccatctggt atggtatgtc cgttgaaatt cgcagtggcc gcaacagtac
    16081 taaatgtctg gcttctacct gtttctgaat aagctttctc ccctgctgct tttgctgctg
    16141 tgatttcttt ttctgtcgga tcaatcagtt gagtcgagga tgtggttgag gttgtctcgt
    16201 tgttgctaga ctgattggtg ctttctatgg actgtatttc agaatagggt ggactatttg
    16261 tctcctctgt cgtttggtta gcaaaatttg acgtttctgt ataaacgttt acataaatac
    16321 ttgataagct ccctaagaaa gtaaaaatca agatgatcga aatcataatg ggatgtaagt
    16381 attttctttt agatacagct ttgagtctag aaacaaaatg tttttgacat atacgttgga
    16441 ttttattttg agttttctta atcattttca tccctcctct caataaaaaa tcatcaaata
    16501 atcctttaca cgttattcta aatttaattt ttattat
//
LOCUS       DAMEET010000061        16343 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.062, whole genome shotgun sequence.
ACCESSION   DAMEET010000061 DAMEET010000000
VERSION     DAMEET010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.062"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(128..823)
                     /locus_tag="PB780_001818"
     CDS             complement(128..823)
                     /locus_tag="PB780_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDE0733870.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            977..1255
                     /locus_tag="PB780_001819"
     CDS             977..1255
                     /locus_tag="PB780_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733871.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1274..2482
                     /locus_tag="PB780_001820"
     CDS             1274..2482
                     /locus_tag="PB780_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDE0733872.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2630..2995)
                     /locus_tag="PB780_001821"
     CDS             complement(2630..2995)
                     /locus_tag="PB780_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HDE0733873.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3142..3915)
                     /locus_tag="PB780_001822"
     CDS             complement(3142..3915)
                     /locus_tag="PB780_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDE0733874.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(4089..4949)
                     /locus_tag="PB780_001823"
     CDS             complement(4089..4949)
                     /locus_tag="PB780_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDE0733875.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4949..6367)
                     /locus_tag="PB780_001824"
     CDS             complement(4949..6367)
                     /locus_tag="PB780_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDE0733876.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSISTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6424..6873)
                     /locus_tag="PB780_001825"
     CDS             complement(6424..6873)
                     /locus_tag="PB780_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDE0733877.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7318..7581)
                     /locus_tag="PB780_001826"
     CDS             complement(7318..7581)
                     /locus_tag="PB780_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDE0733878.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7680..8975)
                     /gene="rho"
                     /locus_tag="PB780_001827"
     CDS             complement(7680..8975)
                     /gene="rho"
                     /locus_tag="PB780_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDE0733879.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8972..10243)
                     /locus_tag="PB780_001828"
     CDS             complement(8972..10243)
                     /locus_tag="PB780_001828"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDE0733880.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10408..11277)
                     /locus_tag="PB780_001829"
     CDS             complement(10408..11277)
                     /locus_tag="PB780_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HDE0733881.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(11569..13179)
                     /locus_tag="PB780_001830"
     CDS             complement(11569..13179)
                     /locus_tag="PB780_001830"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HDE0733882.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(14372..15712)
                     /gene="glnA"
                     /locus_tag="PB780_001831"
     CDS             complement(14372..15712)
                     /gene="glnA"
                     /locus_tag="PB780_001831"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HDE0733883.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(15740..16132)
                     /locus_tag="PB780_001832"
     CDS             complement(15740..16132)
                     /locus_tag="PB780_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDE0733884.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         4664 a         3463 c         2780 g         5436 t
ORIGIN      
        1 ggtaaacggt gttcaatcag ctgaccctcg gtattagctc aaaaaacggc aaaatatgag
       61 gaactatttt tccgtttgtt tctctaatac tcaggtcaaa acgctgattt cacaacctct
      121 ttgttcatca aaagataata ttgtcttcta acaagtccat attcaaaata acgatatgat
      181 ggtctttaac ggtgataata ccatcttttt tcagttgttt taaaattctg tttacactgc
      241 tagcagttga gatgccacaa aatttggcga tttcttcatt cgtgatcgaa aaatcgatca
      301 gcatcccttc ttctgtccgc acaccgaata aatcatataa ttcgtaaagt tgcgtacaaa
      361 cagctcccag ctttccgttt aatagcattt gctgcatttt tttcatcgaa taaagcagac
      421 gatagcggta atactctttt acatagtgtt gtagctttgg gtcttggttg atattttgcc
      481 aaaagcgcac acgattgatt cgataaaact ctgcatattc tgattcaact cgaatattat
      541 acggcgaatc gatgaattgt gaatattcgt cttttaaaag agagacgatt tcaagatctt
      601 tgatatagcg aaggttaaat tctctcccat catttgaaat aacacttgct tttaccgttc
      661 cctctttcaa gatatacgta tgggaatctt ccatcccttc gaatgtcaaa tatttatgat
      721 atcctttgcg tataatcgga aaatcatttt tatccaaata ttcctttaag actctactat
      781 ccaatgatgc tcccccatcc aaactttcat ctaaaaagat catacagtac tttaaaactt
      841 tttaaagtcc aaaggtattt tactaatgat attaacattt gttaagtacc tttttccatc
      901 acttatgcga tttttttcct tttttttatt cgttatactg cagaagtaaa attttttaag
      961 aatagaggga aaaaacatga attactggat gaacacaatc attaatcgat tagaaacagc
     1021 atatcaaaca agatttgata tgaaagcttc tttagtcttt ttaaatgatg cgtatcaaaa
     1081 ctcgatcgaa ttgatcaaag cagtggatga gaatccaaca aacgaatgcg aagaattttt
     1141 aaatctgttt atgtctacaa gagatttatt tattcgccaa cttgttgatc gctatccatc
     1201 caattatcac gatgtagaag ttcaaataca aaaattaaaa gcatattcag cttaagggga
     1261 atgtataaca tacatggaaa agaaattatt taataaatat tttattggaa tcacattgat
     1321 caattttatt gtctacctga tttattatct gttgatggtc atcatggcag tcattgcgca
     1381 agacacctta cacgccagct tgagccaagc aggttttgct tctgggatct atatcatcgg
     1441 aacattgctg gcacgcttat tcatggggaa acagttagaa ctatttggtc ggaagtttac
     1501 cttaagaggc ggcgcgatct tctatctcct ttcaacatta gcatatcttt atacgccaac
     1561 tatcggggca ttgtatttag ttcgattctt gaatggattc ggctacggta cagtttctac
     1621 agctacaaat gctatcgtta ctgcatatat ccctgcatct aaaaaagggg aaggcatcaa
     1681 ttattatgga ttaagtacta gtttagctgc cgctgttggg ccatttttag gaatgatttt
     1741 gctgaatatg actgattttc gtttcattat ctggttttca attgtactgg tcttctttgt
     1801 tacgattgcc tgccttgtct ttcctgtaaa aaatattcca ttatctgaag aacaaaaaat
     1861 atctcttcgc tcatggaata tcaatagttt tattgaaaag aaagcacttt tcattacagg
     1921 aattgccttt ttgatgggtc tttcttattc aagtgtttta tcatttttat cttcttatac
     1981 aaaagtaatc cacttagttg ctgctgggtc atttttcttc gtcgtatatg ctttggtcat
     2041 cacatttaca cgtccattga ctggaaggat tttcgatgtc aaaggagaac aatacgtgat
     2101 gtatcctagc tatttattct taacagcagg attattttta ctgagtgtaa cgacgaatag
     2161 tgtgacattg cttatctctg gagcattagt cggcctaggt tatggtacgt ttatgtcaaa
     2221 tggacaggct gtttgtttaa aaatcgtccg cgaacatcga atcagtattg ctttatctac
     2281 ctactttatt ggtttagacc taggcttagg cgttggtcct tatttgatgg ggatgctaaa
     2341 atctcttact tcatttagag gattgtatgt gattgccgga gtcattccat tgatatgtac
     2401 agcactctat cttgttaatg gccgaaaaca tagtcatgaa gcagagggcg aattgattga
     2461 atcattgaaa aatgaaattt gatcttgatg aatagataaa aaaaaccgtt cagatctgaa
     2521 aataatctga gcggtttttt gagtaaaaat agtttatgat tattcttaat taaacatcta
     2581 tttattttta aagctttcat ttatcgataa gaatagttta actgttgatt tattccattt
     2641 tccgttcatc tggtacgatg ccttgactta gcaaccactt gtggaagaac cattctgcca
     2701 caccgatcaa tagagcagga atcaatactt tcatccaaat atcgactgta tcattcataa
     2761 aatatagata gagccacaag ataactgcag ctgaaccagc atctgcgatc gtcgctgcga
     2821 tgttccctac tttacgtaag atcaacagat cccctagcac atagatcatg actacaataa
     2881 ttaccccaac aatcgttgaa tccataaagc taacaccgta taaacctgtc agcacgaccc
     2941 acagaatcgc ccagatcatg attgccttca ctaccaacgc cttcacatgt ttcattttct
     3001 gcactccttt ctcctggata aaaaagtaag attatctaac aagataacct tttcattatt
     3061 ataccatcgg ctgagagaag cgagcaaaga taaggtccta tctaaaaatg aggaagccac
     3121 ttagaacatt cacgtaataa tttatccgta cagacattcg atatggtgtt cctttacttt
     3181 cgcatactgt tcttttgtca gttctttact tgtcaccaga taatcgattt catgaaaagc
     3241 aatggcttga tgcatgaatt tatgtgcaat tttttgctgt gtaactaaaa cggcaagccg
     3301 gtctgattgt tttctaaaag cttttttgac cattgcctct ccttcatgag tttctgttac
     3361 tccttggctg aaatctagcc cgcgacaact cattatcgct aaatccgcat aatatgacaa
     3421 tgcatcgtta taggcttttg aaccgttgat tgttgctcgt ttcggaacaa ttgcccctcc
     3481 caatacagat acttttgccg ttgtatattc actcaacata gcagctgtga cgatgccatt
     3541 cgtaatgatc tgcaaaccat caaatcttgc taggtttctg actaagtgta aagaggttgt
     3601 actggaatcc aagaataaga ccatatcatc tcgaatcaaa gaagaagctg tatctgcaat
     3661 cttacgtttt tctgtttctt cttctaattt ggtgatcttc atcaggtaag gcacgtcaat
     3721 ttttcgattg tcaataagca tggcgccacc gcgaactcta cggatcaatc cacttttttc
     3781 cagaaaaaca aggtcacgtt taatcgtggc ttcgctataa tttactaact cgatcaaatc
     3841 ggtgactctt gcaaattggt tgttttttag ataattcaca atctcctgct gtctattctc
     3901 cacttcattc aacataaaat caccccattt actttatata gtaacaggaa aaaagaggtt
     3961 gtgacagaag tgattggctt cgagaaataa gaagaaattt ttcgaaaaaa ctacttctga
     4021 acatcgtgtg tggttaagag ggtatgacaa gactattgtc ataccctctt gaccacacat
     4081 ttttattttt agtataaact agccttgcct actgtgttca aaatttccat tttatgtttg
     4141 accacttctt gtaccttttt attagctgct ggataaattg cattcggctc gtacatcgat
     4201 ggattttcac tcaatatttc tctcaaagca ttgaaaaaga cgcttttaag atctgagctc
     4261 aaattcactt tggcaactcc gtattttact gcttcgctga cttctgcgtc cggattacct
     4321 gaaccgccat gtaaaacaaa tggaagatcg atttgttggt tcaattcttt cagtaagtcg
     4381 atatttaatt ttggagttct gtttttcgga tacaagccat gcgctgttcc aattgctaca
     4441 gcaagtgtat cgattcctgt ttctttcaca aaaatcttcg cttgatcagg atctgtataa
     4501 atgattgttt cagcacctcc ttcagcagaa ccattattcc caatcgttcc aagctcagcc
     4561 tccactgaga catttaccgc atgagctaac gcaaccactt ttttcgtaat ctgtacattg
     4621 tcgtcgaatg atttcaaaga cgcatcgatc atcacggaag aatagccgtt tcgaatcgcc
     4681 cgtaagacat cttctatcgt accgccatga tccaagtgga taacgatcgg cagtttactt
     4741 gtataagcat aggctcttac tgccgcaaca aactcatcac ttaaataggc gatttcatca
     4801 ggatgaattt ctaagattac tggtgcttct aatctctctg cttcttcgat gattgctttc
     4861 atcatatcat agctgcaaat attgaaggct ggtactgcaa attgattctc tttagccact
     4921 ttcaataact ctttcattgt atataacatt acgcttccac ctcatttgtt agattttcat
     4981 ttttcgtcca cttgtcttgt tttttctctt tcactgtttt tttcaatagt ccaagcataa
     5041 aggctgtagt gactgtacct gatgcaattg ccaagacata cataggtaaa ttgttcataa
     5101 catttggaat tagcatcacc caaatccctc catgaggtgc cattgaagta actcctactg
     5161 ccatggaaat ccctgcagcg actgctgaac ctgtcatcaa actaggtatc acgcggatcg
     5221 ggtcagatac cgcaaacggt atagcacctt ctgtaatata cgaagcgcct aaaatccagc
     5281 atgattgacc tgaagtccgt tcttctttgg taaatttatg tttgaataat aaagtggcta
     5341 aagcaatacc taggggaggt gtcattcctg cagccataca agcagcaatc ggtgcattta
     5401 ctccagctga catcaaaccg atcgagaaag tcgaaatcga tttattgatc ggcccaccca
     5461 tatcagctgc catcatgatc ccgataagta atccgaacaa tagcccggat gtatttccca
     5521 agctattcag ccaatttgtc aaagcatcca acacccatgt gatcggttgt tcaatcacaa
     5581 ataccatcaa aagcccaata attaagatcg ataaaaaggg gacgacaatc agctgataaa
     5641 tagcagaaaa ttcttttttt acgctaattt tatttgccag aaaatcacag atataccctg
     5701 ctagcacgcc accaatcatt ccgccaagaa aacctgaacc acctgtagag gccagtaatc
     5761 cggcaacaat tcctggggca aaacctgctt ttccagcaat cgatgtagca atccccgcgc
     5821 ctaacgcagg aaccatcatg gcaaacgctg tatctcctcc gatccttgaa aatgcagctg
     5881 ctaacacatt atagtctgct gagttgggat cagccgctgt aatcccaaag gcaaaactca
     5941 gtgcgatgat gattccgcct gcgatcacaa atggaagcat gtaattcaca ccattcatga
     6001 aatgattata gattgtcggc tttttctcat ttgcagctgg ttctttttca ttagacacaa
     6061 cgaatacgcc aatatctctc aatgcttcgt ctattactac ttgcggttct ttgattgctc
     6121 ggcttgtaga gacacgcttt acttttttcc cggcaaatcg gtccaattcg atttctttat
     6181 ctactgcaag aatgattgca tctgcctctt tgatttcttg ttcagataac tgattttctg
     6241 tttttacacc ttgtgtttcg aattttgctt gatggtttag atttcttgct gttacttcta
     6301 atttttctgc cgacatataa gtatgagcaa ttccagtcgg gcaagcagtt acaccaacga
     6361 ttttcatgat tattttcctc ctttttcttc actatcgata ctcggacaaa gactctacta
     6421 ttttcattct tgactaatct gtctgatgat atctgctggc gtagtggctt cttttattcc
     6481 ctttctaaaa tctgcattca tcaatcgtct ggagagtgtg ctgagaatct ctagatgcat
     6541 atctgctgat ttttcaggaa ctacgatggc aaaaatatat tcaattggca tttcatctat
     6601 cccgctatag tccgttaatt tggctgtatg aaaaaaacac agtgctggtt ttgtcacata
     6661 tttgcttttt gcatggggaa tcccaaatcc atcttctatt cctgtggaga tctcctcctc
     6721 tcgtttgaat agtgccttaa cgactttttg aacatttctt accttatttg tttttttcaa
     6781 cacatcccct attcgctcaa ataatgatcc ttttgaatct atcgatggat cgaaaacaat
     6841 cagttcttct gcgatcaatt gatcaaacct cataaaaaac ctctccccca caaaaaatgt
     6901 taacgttttc ttggaactga ggtcagtata gcatcgctag ctgtaccgtt caatattatg
     6961 aatgaacaga ggaaaaagac ccatcatccg cgtgtataag tcattaaaat gaacgatcta
     7021 gctcacaaac aatcgcaagt ctataacaaa acaaaaagcc ttccttgaaa ggaaagctta
     7081 gcttctaatt ttaggtaaat ggttttttac ttcgaaataa cccctcttca tttcgttaat
     7141 ataaggtgtt tttatcaagt ggataattaa aactatatac tagtttttca ttgttttgtg
     7201 cattatgatc ctacgacttt ttcagcatca aacgaagttt taaattggca aaaaacataa
     7261 aaaaacagac tatccctatc agatagtctg ctaaacgttg agttatcagc gtttttatta
     7321 ttccgcagcg tttgcgtctt tgagaccgta ttttttgttg aaacggtcca cacgtccgtc
     7381 tgcttgtgtg aatttttgac gtccagtgta gaatggatgt gagtcagaag ttacttcgac
     7441 acggatgact gggtatgtat taccgtcttc ccattcaact gtttcttgtg aagtttttgt
     7501 tgaacctgac aagaatttga aaccagttgt tgaatccatg aatactactg ggtggtaatc
     7561 tggatggatg ttttctttca tgattttttc gctcctttgc cctgctccat ttgctgaacc
     7621 agagttgatt tgtcgattta caacattaac atcttaacat gtttcagtca aggttgcaat
     7681 tatcttttca gatttctggt cggatttttt ttaccaaagg agacatcttg aaattctttg
     7741 aaaaattgct gattgttttt tgttttacgc aaaaagcgga tcagttgttc cgtatattct
     7801 aatgaatctc cagtcatatt ttttcgaagt ttccaaattt cttctaattc ttcacttggc
     7861 atcaataatt cttcttttcg ggtacctgat tttttgatat cgatggctgg gaagatccga
     7921 cgttcagata attcacgtga aagttgtaat tctagatttc cagttccttt gaattcttca
     7981 tagatcacgt catccatacg gcttcctgta tcgactaaag cggtcgctaa aatcgtcaaa
     8041 ctgccacctt cttcgatgtt tcttgctgcc ccgaaaaatt tcttaggttt atacaatgcg
     8101 gctgggtcaa tcccgccact caatgtacgt ccgcttggtg gaacgactaa gttataggcc
     8161 cgagcaagtc tagtaatact atccatcaag ataacgacat ctcgtttatc ttcgactaaa
     8221 cgcattgcgc gttccaatac taattcagaa actcgagtat ggttttgcgg ctgcagatca
     8281 aatgtagaag ataccacgtc gcctttcacg cttcgttcta gatccgttac ttcttccggt
     8341 cgttcatcaa tcagtagaac gattaattct acttccggat agttttcggt gataccattt
     8401 gcgatttctt ttaatacaga agttttccct gcttttggcg gtgcgacgat caatccacgc
     8461 tgaccaaaac cgattggaga aaagatatcg atcattcggg tagatagtct ttgcggtgtt
     8521 gtttccagtt gaagctgtct ttgcggataa agaggtgtta aggctggaaa atgcggacgt
     8581 tcttttgctt cttccggatc ttttccatta acgctttcca cgtgcatcaa accataataa
     8641 cgttccgatt ctttaggtgg acgggctttt cctgccactt tgtccccatt tctcaaactg
     8701 aatcgtctga tttgtgaggc agaaatatag atatcttctg cacttggtcc ataattgatt
     8761 ggccgtaaaa aaccgtaacc atcttgacca acgatatcca atattccttc catataataa
     8821 aatccttgtt tctctgcttg tgcacgaatc acagctagag ataattcctt tttattcatc
     8881 ttactgtaat aaggaatttt aaattctttg gcataactat aaacttcttt taacgttttg
     8941 ttctctagtt ctgccattgt caaataatcg ctcattcagc tcccacttcc tctatcattt
     9001 caattgatgc ccctaaggct gttagtttct cgataatatg atcataaccg cgtaagatat
     9061 aatctacatt gtaaatcgtc gttgtccctt ctgccaatag tccagcaatc actaaacaag
     9121 cacctgcccg aagatcagaa gccacgactt ctgtgccgtg aagtttattt ggaccattca
     9181 cgatgatcat atttccttcc acggtaatat cagcgcccat tcgtgctagt tccggcacat
     9241 ggttcacacg tttcgcatag atcgtatcga tgatttctga agtgcctgta gtcatcaata
     9301 gtaacgcagt aagtggctgc tgcagatctg tggcaaatcc tggataagga taggtcatga
     9361 tgtttgcggg ttttaattct ttcgaggggt aaacttcgat ttcatcttcg ctgattttca
     9421 tatggacacc aatttcctgc aattttgcta taaagctttc caaatgttca aagatcacat
     9481 ttttcacttt gataccattt cctactgcag ctgctaatgc tagataggtt cctgcttcga
     9541 tgcggtcagg aatcatgaaa tgccgacagc catgtaatgt ttcgactcct tcgatacgga
     9601 tcacatctgt acccgctcct cgaacttttg cccccatatt attcagtaaa gttgccacat
     9661 ctatgatttc cggttctcgt gcagcgtttt caattactgt ctgtcctttt gcttttacag
     9721 cagctaacat cacattgatc gttgctccga ctgaaaccac gtccatgaaa atccgattgc
     9781 cacgcaatcc tttattttct gtccgtaaat acatggcacc atgttcattt gtcacttcag
     9841 cacctagagc ttcaaatcct ttgacgtgta ggtcaatcgg tctagggcct aaataacagc
     9901 cacctggcag gcctactacc gcttcgccaa atttaccaag cagtgttccc ataaaataat
     9961 aagaagcacg caaactattg atttttccgt gcggcatcgg aatcgatacg acttctcgag
    10021 gatcgatttc tagtatgttg ttggaaaaat ggacagttgc gcccatgatc tctaaaattt
    10081 caattaatga atgaacatct tgtatgtccg gcacaccttc caagatcact ggggaatcag
    10141 ctaagattgc agctggaatc aatgcgactg cactattttt tgcaccgctg atcgttactt
    10201 caccagctaa aggtcggttt cctttgatta cgattttttt catctgtttc caccttgttt
    10261 attaatcttt tttcatggat atgcttgtat tttatcatag ataaaaaaaa gaaacgagca
    10321 tggtctgccg ttttaagtaa aaaagaatgg agagcgactt caaaagtcgt tctccattcc
    10381 tatatatcgt atctgttatg tggatgctta cgctttgtta gctgaaccga accagtcgat
    10441 atgagattta gcatcttcaa cgattgctgc tttacctgga gctaataatt tacgagggtc
    10501 gaatccttta ccttctttat ctttaccagc ttcgatatat ttacgagtag ctgcagcaaa
    10561 tgataattgg aattcagtgt taacatttac ttttgagata cccattgaga tagctttttc
    10621 gatttgatct tgaggaatac ctgatccgcc atgtaatact aaaggcatgt ctggacctac
    10681 agcatcagca attgcttgta agtggtcaaa agctaatcct ttccagtttt ctggatattg
    10741 accatggatg ttaccgatac cgcaagctaa gtagtcgata cctgtagcaa ccatttgttt
    10801 gcattcttca atatcagcta attcgccgtt accgatgatt ccgtcttctt ctccgccgat
    10861 tgaaccaact tcacattcaa ctgagatacc ttttgcatgt gctttttcaa caacgtcttt
    10921 agctaatttc aagttttctt caaatggtag atgtgaacca tcgaacatta ctgaagtata
    10981 gcctaattcg atacattcca aagcagcttc gtaatcacca tggtctaagt ggatagcaac
    11041 tggaacagtg atgttcattg cttcaactaa atcagtgatt aaatctttac agattttgta
    11101 accacccatg tatttagctg cacccattga tgtttggata agtactggtg cttgttttgc
    11161 ttcagccgct tcaaggattg cttgagtcca ttccaagttg tttgtgttga atccgccgac
    11221 agcgtaaccg cctttacgag cagcttttaa aaattcagct cctgatacta ctggcataat
    11281 aaaattcctc ctaagttttg taaaactaaa tttgttaaga caattcttaa ccaacgaccc
    11341 tattttacca aacatttcgg tagttttcta ctaaaatcaa tgaaaatgaa aattttcaag
    11401 aagttttttg caaaaaataa aatatccaat agcgctttaa tcgaaaaaag tcttttgctt
    11461 tctatctttt ttcctacaaa taataacaaa tcagaaaaag agaaaagtga caagaaaatt
    11521 gcttatcccc ttctcttgct acttttctct ttctatcaag ttcaaaaatt attttgtttc
    11581 gtgatctgct aatgatgctc cgacaaatgc tttgattaat ttttgcgggc gatttggacg
    11641 agaaatcaat tctgggtgga attggcatcc cacaaagaat tttttctctg gcagctctac
    11701 gatttctact aaacggttat ctggtgagac acctgaaaag actagccctt gttcttcaaa
    11761 caattgacga tatttgttat tgaactcata gcggtgacgg tgacgttctt gcacaacatc
    11821 ggctccatca tatgcttcag ccgtcttcgt tccttttttg attttacatg ggtataatcc
    11881 aaggcgtaaa gttccaccca aattctcgat gttttcctga tcagccatta aatcaataat
    11941 attgtttgct gtatctggat tcgtttcagc tgatgacgca tcttctaatc caacgacgtt
    12001 acgtgcaaat tcgacacaag ccatctgcat accaagacaa atacctaaaa acggaacgtc
    12061 attttcgcga gcataacgaa tcgcttcgat tttcccttct actccgcgat caccaaatcc
    12121 accaggaacc aagattccat ctgcgtcttt caaacgttct tctacatttt ctctcgttaa
    12181 ttcttgtgct ttgatccaat cgatctcgat gtctgaatta taggcgaaac cagaatgttt
    12241 caatgcttca actactgaga tatatgcatc tggtaactcg acatatttac cgacaagcgc
    12301 aatccgtact tttttcttca agttcaagac acgttcttct aattctttcc attctgtcat
    12361 gtctgcttct ggtgtatcta gttttaaatg gtcgcaaaca atttgatcca ttccttgtgc
    12421 ttgtaaaact aatgggatgg agtaaagtgt atctacatct ggcgactcga taactgcttc
    12481 tggttcgaca tcacaaaatt gtgccaattt gtttttcacg ctttgagaaa caggttgctc
    12541 tgtacgtacc accaaaatat tcggttggat acctaaacca cgtagctctt ttacactgtg
    12601 ctgcgttggt ttagttttca tttctccagc agctttcaag taaggaatca atgttgtatg
    12661 gatatacata acattatctg taccgacatc tgctttcatt tgacgaagtg cttcaaggaa
    12721 tggcaaagat tctatatccc ctactgttcc ccctacttct gtaatgatca catctgaatc
    12781 agtcatttta gctgctcgca tgattttttc tttgatctca tttgtaatat gaggaataac
    12841 ttgaacagtt gctcccaagt attcgccctt acgttcttta cgcaaaactt cagaatagat
    12901 ttttcctgtc gtcacgttgg agtatttgtt caaattgatg tcaataaaac gttcgtagtg
    12961 gcccagatcc aaatctgttt cagcaccatc atctgttacg aatacttctc cgtgctgata
    13021 tgggctcatc gtccccggat ccacgttgat gtatggatca aatttttgga tagttacttt
    13081 taagccgcgg tttttcaata agcgtcctaa agaagctgca acaatccctt ttccgattga
    13141 tgaaacgaca ccgccggtca caaaaatata ttttgtcata agttcaaaaa ctccttttta
    13201 atttatgtgc aggaaaagtc tcagacaata aacaaagctc cctattcttc aataagaata
    13261 gggagccgaa atttctgtaa acttctgacc ctctaaaagg gtgcccaagg tgtataatac
    13321 aagctgacta agggaaagtc aagtactcaa atgaatacta tccgcttaca attttttctt
    13381 tttcgatatc ctttgagaaa ttatttctta agtcctttat gagtgcaaaa aaaatttaac
    13441 ccacataata agcttgatat aaacatagct aacgttaaac ctaagcaaat cagaaaatcg
    13501 ttactgaaaa attttaagtg aaataataga taaaaaaata aaacaaacat tatgatagat
    13561 aatatcccac ctaacaaaga actacttttt cctacttgca gcaacagttt atatagctta
    13621 tcgtagaaac aatctttttc tttcaccctt atttacctcc tttacgtaaa aaacgtagtc
    13681 cacagttctc ggatttttgg catatctttt ctattaaata atatcaaaaa caaaacaatt
    13741 ccactaattt tttcaaagat gacctttttt tcaacaaaag tgccttgtag ggattcattg
    13801 aaggtaacga atcacataag taaaaaataa ggagatgcga caaaagtcgt gcctccttat
    13861 ttctaaataa acggtgttct cctgccaact cctcggtcac aagtcacaat atccatcaaa
    13921 catgagaagc tgtttttgaa attatttctt gtgattcgga gttaaatggt aggctcacaa
    13981 cctctggata aacggtattc aaaagccagt ttctcggtat taagccaaat ttatgcaaaa
    14041 atgtagaaag ccattttcac aaaatttttc ttaatactcg aaactaaacg tctttttcaa
    14101 aacctcttgg taaacggtgt tcaaaagctt gcatctgcgg taataagttc agccaattca
    14161 aaaacaagaa aagctgtttt tgcttggcta ttcttattac ttgttgcagg gcagcttttt
    14221 cacaacctct ccactaaacg gtgttcaatc agctgaccct cggtattagc tcaaaaaacg
    14281 gcaaaatatg aggaactatt tttccgttag tttctctaat actcaggtca aaacgctgat
    14341 ttcacagcct ctaattcacg gtattcctgt tttaataaag ttctagatat tgttctcttt
    14401 cccactcgga aacagtttgt ctgaatgccg cccattcgag acgttttgct tcaacaaaat
    14461 ttgagaagat atgttgtcct aatgcgtcaa tcattacatt atccttacgc aattctttga
    14521 tagcattatg gatagttgaa ggtaagtcct ggatatgtgc agcttctctt tcttcctcat
    14581 ccattacata aatgttacgg tcaacagctg ctggcggtgt gatctgatta cggataccgt
    14641 ctagtcctgc ttgcaacaat actgccattg ccaagtatgg gtttgcagat ggatcaactg
    14701 accgcaattc caaacgtgtg gataaaccgc gagattctgg tacacgtact aatggcgaac
    14761 gattacggcc actccatgcg acataaacag gcgcttcata acctggaacg agacgtttat
    14821 aagaattgac tgttggattg caaacggctg tgtaggcacg agcatgtttc agcaaaccac
    14881 ctaagaaatg ataagctgtt tcacttagtt ccaattcacc gtctttatca tagaaaacat
    14941 ttccttcatc attgaacaaa gacatattgc aatgcatacc agaaccattg atcccaaata
    15001 acggtttcgg catgaaagtc gcgtgaagtc catgcttgcg tgcaatcgtt ttgacaacga
    15061 gcttgaaggt ttggatatta tcacaagcat ctaccacatc tgcatattta aagtcaattt
    15121 catgctgtcc tggcgctact tcatgatgtg aagcttccac ttcaaagcct agactttcta
    15181 attccaacac gatgtcacgg cggcaatttt cacctaaatc tgttggtgca aaatcaaaat
    15241 aaccaccttt atcattcaag gtcgttgtga tcccaccgtc ttcgtctaat ttaaataaga
    15301 agaattctgg ttcaggtcct aaattgaaag aggtaaaacc aaggtcgcgc atatctttca
    15361 atgctcgttt caaattcccg cgaggatcgc cggcaaatgg tgtcccatcc ggattataga
    15421 tatcacaaat taaacgagca acttttccat gagcactttc ccacgggaag atcatccaag
    15481 tggagagatc tggatacaaa tacatatcgc tttcttcaat tcgaacaaat ccttcaatcg
    15541 aagaaccgtc aaacatcatt ttattttcta atactttgtc gatctggctc actggaactt
    15601 ctacattttt gatcgtacct aagatgtctg tgaacatcaa tcgtaaaaat cgtacgtttt
    15661 cttcctcaat gattcgtttg atttcttcac ctgttatctg tttctttacc atggtatccc
    15721 tcttcctttt ttaatttatt cagtcaaaga attgtctttg caatccttgc gactgataat
    15781 gacctgtttt gcttaaccca ccttgcgcca aaatctcatc acgaaaaatt tgtcgaacgt
    15841 catgatctgt caacggcttc ttgttttttg cttcttgttt tttcgcctgt gccatttcgt
    15901 atacacgttt gatcccagcc atattcaatc cctctgaaag atagtcttta atctcaagca
    15961 acatatcaat atcattcagc gaatacatac gtcggtttcc ttcgcttcgt tccggatgaa
    16021 ctaaatcttg ttcttcataa tagcgaattt gccgagcaga caaatcagtc agcttcataa
    16081 ctgtaccaat tggaaatacg gacatagatc ggcgcaattc cttttctccc atcagacgtc
    16141 ccctttcgtt ttataagttg gctaaagtgt accaattaaa aaaaatattg tcaactgcat
    16201 atgttaggtt ttctaacatg agatctctat tttttataaa ataacaaaaa aataagaaca
    16261 caaaaaaggt atctagttga ttttcgtacc agataccttt tattttttat tatactgtac
    16321 tatcctttat tagtgttttt att
//
LOCUS       DAMEET010000062        16336 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.063, whole genome shotgun sequence.
ACCESSION   DAMEET010000062 DAMEET010000000
VERSION     DAMEET010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.063"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(239..523)
                     /locus_tag="PB780_001833"
     CDS             complement(239..523)
                     /locus_tag="PB780_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733885.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(541..1431)
                     /locus_tag="PB780_001834"
     CDS             complement(541..1431)
                     /locus_tag="PB780_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDE0733886.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(1451..1669)
                     /locus_tag="PB780_001835"
     CDS             complement(1451..1669)
                     /locus_tag="PB780_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HDE0733887.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(1639..3339)
                     /locus_tag="PB780_001836"
     CDS             complement(1639..3339)
                     /locus_tag="PB780_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDE0733888.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            3606..4379
                     /locus_tag="PB780_001837"
     CDS             3606..4379
                     /locus_tag="PB780_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733889.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(4460..5029)
                     /gene="thiT"
                     /locus_tag="PB780_001838"
     CDS             complement(4460..5029)
                     /gene="thiT"
                     /locus_tag="PB780_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HDE0733890.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(5095..5197)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5203..6174)
                     /locus_tag="PB780_001839"
     CDS             complement(5203..6174)
                     /locus_tag="PB780_001839"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDE0733891.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(6268..7641)
                     /gene="glmU"
                     /locus_tag="PB780_001840"
     CDS             complement(6268..7641)
                     /gene="glmU"
                     /locus_tag="PB780_001840"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDE0733892.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(8144..8962)
                     /gene="purR"
                     /locus_tag="PB780_001841"
     CDS             complement(8144..8962)
                     /gene="purR"
                     /locus_tag="PB780_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HDE0733893.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(9129..9953)
                     /locus_tag="PB780_001842"
     CDS             complement(9129..9953)
                     /locus_tag="PB780_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDE0733894.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(9926..10606)
                     /locus_tag="PB780_001843"
     CDS             complement(9926..10606)
                     /locus_tag="PB780_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDE0733895.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(10629..11567)
                     /locus_tag="PB780_001844"
     CDS             complement(10629..11567)
                     /locus_tag="PB780_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HDE0733896.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(11812..12675)
                     /locus_tag="PB780_001845"
     CDS             complement(11812..12675)
                     /locus_tag="PB780_001845"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDE0733897.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(12817..15390)
                     /gene="mprF"
                     /locus_tag="PB780_001846"
     CDS             complement(12817..15390)
                     /gene="mprF"
                     /locus_tag="PB780_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDE0733898.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            15531..>16336
                     /gene="proC"
                     /locus_tag="PB780_001847"
     CDS             15531..>16336
                     /gene="proC"
                     /locus_tag="PB780_001847"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDE0733899.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
BASE COUNT         4776 a         3497 c         2804 g         5259 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttactta ataccgagaa aatggttttt gaacatcact tattcaaaat
      121 aaaaaaagat tgtgacacga attttgtcac aacctttaaa tgtattccta gagcaaagaa
      181 gctaagcgaa gaaatccaat tctgattatt tattgtattt ctttgttcag catccttgct
      241 aaaagcccat attggacaaa gtattcacta cacccatgat ctcatctttt cgagaacgtg
      301 ctttttctgt cggatcttct acctcttctg tgtcaattcg aacaataaat ttccgattca
      361 tcgtcacgta agcaccgtcg ttgatcaaaa taaacagttc gttctcatta tagtctgcac
      421 aggcatcttt ccatacgtca aagccgtttt tttcagaagg gatatcccgt ttaaccacag
      481 tttttccact taattcgtgg aacgttacgt cataatattt cattatctct acctctttcg
      541 tcattcaata ttttgtactt gttctctgat tttttctaag atcgtcttag actggacgac
      601 ttgattttta atttcgattg catttgattt ggaaccgatc gtgttgattt cccgattcat
      661 ttcttggatc aggaaatcta attcccgacc tacaggacat tgtgcttcga taagttcatc
      721 tagtttttgt acatggatcg ccagacgatc cagttcctcg tgaatatctc cgcgttccaa
      781 caagatggct agctccgtca acaaccgttg ctcatcgact ttcgctccaa gaaattcctc
      841 cagctttttt tgatacctta tttgaaattc cttttcatag atttctacaa aactatcgag
      901 tttttttagt acttctttga attcttcagt attctttttc aacactaaag ctaaagcttg
      961 accttctttt tctcgactcg cctcaataga atcaagtgcg cttttcaatg tatctagagc
     1021 taactgtgcc aattcttcca tgctctcttc cggtttttct tctaccgtta cgaagttttc
     1081 gtccagcgcg aagcgttccg ccatccgact aattgacact tgattttgac taccgtatcg
     1141 ttgttgaatt ccgtctgtca attgagtgat aagtgaatca atcaattccc agttgatgac
     1201 gatctgtttt tcgtttttgc caatataagc tagattcaca aatacttcga ttcgaccgcg
     1261 gcttaactgc tgtttaataa gctgacgaaa atcgttttca acaaagttca ggattttagg
     1321 acttcgtatt tgaacatcta gaaaacgctg gttgacactt tttatttcaa tatctaactg
     1381 atacgctttt gtttcgcgga tggctttgcc aaaaccagtc atacttttca ttcgacaggg
     1441 tccttcctca ttacatattt tctattgctt tttgcagttg ttcaaactct tcgttcgtca
     1501 atgtcaggcc tttccccatt ttttcatggt ctggcgccca atctcgaaga tcgaattttg
     1561 gcgggcgacc attccagctg actaggttca gttctttacg ccaccccttg ttgttttctg
     1621 acaatatagc gatttcttct acgatttcat atgaaaattc ttgtgccatg gttgttcctc
     1681 cttgagataa tataaatata ggatcagtaa tgtgtcaact ggttgcttaa gcaattcatg
     1741 ctgcaaacga tccttgcgtt ttccttgtag cagataatca aaataaacag ttttcgtgcg
     1801 ctcattttgt attttttgct ggtattttcg ataccttcgc cattcttcag tacttaattg
     1861 accaatttgt gccaataaat catccggcat actttttata tcttgttggc agatcatttg
     1921 aatcatcatg tctacttgat agatttcatt gggcagtaaa aaatgttttt ttgctaaaag
     1981 ttctagcatc gccaatccgc atgtctctgg tacgaccctg ttcttatata aattacgcag
     2041 tttttgccaa aattttatgc ttttgaaaaa agataattct aactgaattt cttttgttaa
     2101 gtaagacaaa aatcctcgcc agtatgttct tgcaggttta acagaatgaa atgtatacca
     2161 gttcccatag taatagccta atcgtttttt catttccaga tagcctaatt gtaaatttaa
     2221 ttggttcctt tggctatcaa aaagcagaaa gctgcctagt gtccacaatg tttgacattt
     2281 ccaatggata aaaatgtctg gcatacgaat ttttttcgct gggcctggcc cttgcacatc
     2341 tacgacaaat gcctctgttg ctcctttgtt aatcgcaata tcaataggta ttttgttgcg
     2401 atacccaccg tctgcatatt tattttttcc gatctttcga taagccatag cgggataaaa
     2461 tgaagcagag gcgagtatcc aatctggtat ttcttctgtt tttgtctgtt ggatatcagt
     2521 cactacttcc gctagttctg gcagtttagt agacaccgta tataaacgta ttttgggatt
     2581 atgtttgagt aaggacaaat ccaaattgtc tttgaccatc ttttccaatg gagcagaaga
     2641 aaccccgcct tcaattagtg ctgttttcgt catttggcgt ctttctcgat agaagcgttc
     2701 acgaaggtct tcaactgcag ccatttctgg taacgcaagc acttgtcttg tcgacagctt
     2761 tttccataaa ttcacagctt ttttcatatc tccttgtaaa atcaatgcgc cgttcaacgc
     2821 acctaccgag gtacctgtga taatctcaaa gttgatatca tgttccttca gcgcctgcca
     2881 cacaccaatc tgataagcac cgtgcgctcc tcctccacct aaaacaagtg ctgtatgata
     2941 gaccagcttt ttatttaatt gtgtggttcc ttgatagccg tttttatgaa aaatacgcaa
     3001 ccattcttct gatagaggag attgaaataa aaaacaacag ttttctttaa taaaaaaacg
     3061 agcgcaagct tccatatacc gaaaaaattt tttccattca aaagtttcac cttcaatcaa
     3121 cagattatta aaaataacat ctcctctact tttagcaata gtaaaataga cggtatattt
     3181 tttcgatact gctttaaaag cacattgctt ttttgcagaa cgccaaaaag cgtctctact
     3241 ttcttgatga ctttgaaaaa atggaagggg ttgttttgct tgctggatag gcgaatacag
     3301 cgacattttc tgtgaaaagt aaactttttt tatctccatc gcgctctcct ttttcttcaa
     3361 gtataccagt acgtcataaa aaggtcatga gtctcgtatt cgactttgta gattccataa
     3421 gaaaacaatt cataaaacct cctttaaata aaaaaataca aaaatcacaa aaattaattg
     3481 aaaatgagag tgttttcaat gaaaacgtga ttttgtaatg gaattttaaa ataaaagaaa
     3541 caagcttttt taatgcaaat gttgtaaaat aaaagagatt aagaggaaat aagaggagga
     3601 acatgatgac tgagtcagtt tatgtacata ttgataccac aagtaatgct gttttaacaa
     3661 aagggctgac tgctactgac tttgcaaaca gtatcgtgca ttttcctcaa aacttgctct
     3721 tgcttgatcc atctgcttct gctggagaat atgaatccca tacagggctg aaagtaatcc
     3781 gtggtacaga aaatattcag cgtttctttt cctcttctcg taacaggcgt ggattcgatg
     3841 aattgaagtg gatcgacttt actgatttaa cgatgctgaa agaattgaca cctttagaaa
     3901 tttcagaatt attatatttt ggtcatatga aaacacatct tcattcgccg tttttttata
     3961 aactacagaa taattttgta tacttcgatc ttaatgatca gctgaatcgg atttattatc
     4021 gctatttaga tgaattttat aggatttttg ctagtaaatt aagttcgatc ttgtcagata
     4081 agatcaatgt aaaaaaatct tttttcaaaa aagaaacacc tgttgaaaaa atcagtgtgg
     4141 aattattgaa agaaatgcgt aatttgatgc aggaagggat cattttttct tttgatcaag
     4201 ttggtttgat cgatggtaaa tatcgtatcc caatctatgt gattgaagat tatatttgga
     4261 aattaaaagg cacccggtat cgagaagagg aaatcatcgc tactttaatc tactctgtca
     4321 ccgatcgaca ctggcaattg gtagaagatg ccgatctttc ttatctgcca ttgaagtaga
     4381 tctatttctt atcaaaaaat aaaaaggaaa caagaaacta ggacatgatt ctgtctcaag
     4441 tttcttgctt cttttttatt tacagctcct gtcgtttatc tggcacaaaa atcgaaggag
     4501 tcgtttgttg gatccctaat aaaacgatga tcgttacaat agctgtactc aacccgctgg
     4561 caccattcat tacgaaagaa aagagccaag gattcatccc ccatagtgca tacgctcccc
     4621 agaaaaccac tcctgcaata aaatgccaaa aatatctgga aagtgcgcca atcaatgcac
     4681 caaacacgat gctttttcta atttgtttcg tattatcgtc tacatagcct ttttggacat
     4741 ttgaagccat gagccctgct gcgcctgcaa aagtataggc aatcgggtac tcaatcaaca
     4801 cttgaacgac acttaaataa taaacttgtc ccaacggaaa atgtaggatg ccccaaatga
     4861 aacctgccag catccctgcc tttgtccctc ttcttaacgc aaaaagagtc aatggaatct
     4921 gccctaatga aatagaaaaa ctgctgccga tctggatcgg aatcaacgac aacaccatcg
     4981 ctaacgctgc aacaatcgtt ccttctaccc aaactctatt ttttcccata aaaaaacttc
     5041 ctcctttgaa taaaaaagtc acaaaggagg aagcagcttt cgaaaatagt caaacagctg
     5101 tttattcaca attcctacgc tcgcataacc gatacaggta cgaagggttt agaaatagat
     5161 tcaatctcag cccaattgga ctcccctttg tgagtttatt tattatttat tttttgtttc
     5221 aaacaatgga ctaactggtt tgttttcatg gatacgtttg attgcatctc ccattagtcc
     5281 gccaacactt acttcatcga ttttgtcgat tttacgatct tctggaagat agatcgagtc
     5341 tgtaacaact agacgctcga tagctgaatc ttctattctt tgtaaagcag ggccggataa
     5401 tactggatgt gtacatgaag catacacact agttgcacca gcttctttca aagcattggc
     5461 agccaatgtg atcgtacctg ctgtatcgat catatcatcg atcagtacac aagttttgcc
     5521 tttgacttgt ccaatgatat tcattacttc tgcaacattt gcttttggac ggcgtttgtc
     5581 tatgatcgca atcggagatt tcaaaaattc cgctaattta cgtgcccgtg tgacaccacc
     5641 atggtctggt gaaacaacga caacatcatc gcctttgatt cctttttcta agaaataatt
     5701 ggcaataagt ggcgcaccca tcaaatgatc gactggaata tcaaagaaac cttggatttg
     5761 aactgcatga aggtccaaag tcagcattct tgttgcacca gctttttgaa tcatgtttgc
     5821 tactaatttc gctgtgattg gttcccgtgc acgtgcttta cggtcttgac gcgcatagcc
     5881 ataataaggc atgactacgt tgattgtttt tgcacttgct cgtttcagtg cgtcaatcat
     5941 gatcagcaac tccatcaagt tatcattgac tgggctgctc gttgattgaa taacatagac
     6001 atgcgatcca cgaatacttt cttcaatgtt tacttgaatt tctccatcac taaattgagt
     6061 aacagatgat tttcctaact cgacgccgac tgctgctgca attttctctg ctaatgggcg
     6121 attggaattt aacgcaaaaa tttttaatct tgggtcaaag taatgttttg acatggggac
     6181 ctccactttc tttttcacaa ctctagaatt ctacctaata aatagtagtc atatttttta
     6241 aataaatcaa gtgattttgc aaaagtctta gtttagataa gggagttttt ttgcatatcc
     6301 ttctttattg acttgtcttg ctctcgcaat tgccagcgca tattctggaa tattgtctgt
     6361 aattgttgac ccggcagcaa tcgaactgtt tttagctacc tcgacaggtc cgatgatatt
     6421 cgtgctagaa ccaataaagc tgtggtcgcc gacagtcgta tgatgtttgt tctttccatc
     6481 atagttgacg aagacaacac cacatccgac attgatttct tctcccagcg tagcatcacc
     6541 aacataagtc aagtgaccga ctttagttcc tttgccgatt tgagcatttt tcacttccac
     6601 aaagtttccg atatggacgt tttcgccaat ttcagctttt ggtcgtaaat gtgcataagg
     6661 tcccacatct gcatgtttct tgacatggct gctttcaatg actgagtttt caatgacgac
     6721 gtgatcctca atcctactgt ctacgatttt tgaatgtgac ccgatgacac aatcagaacc
     6781 aatgactgta tttccttgga tttgtacacc tgcttcgatc aatgtgtctg gaccgatttc
     6841 tacgcctgca tcgatgtatg tcgttgctga atcgacaaag cttacgccat tgaccatatg
     6901 catctggtta attcgctttc tcatgatctc atttgcttta gctaacgcga tccgatcatt
     6961 gactcccatt gattcttcaa aatcatccgt ttgataagca gcaactgtat ggccttcttc
     7021 ttttaagatt tcgatgatat ctgttagata gtattcacct tgagcattgt ttgttccaac
     7081 tttgcttaac gcatcgaaca aagcttcgtt gtcaaaacag taagttcctg tattgatttc
     7141 ttgtacaaga gcttcttctg gtgttgcatc tttttgttcc acgatttttt ctacgatccc
     7201 aatatggtca cggatgattc gtccatatcc tgttgggttt tctgcttgtg ccgtcaagat
     7261 cgttgcactt gcattttttc cttggtgata ttcaaaaaga ttgttcagcg tttcagtagt
     7321 caataatggt gtatcgccac tgataactaa cgttgttcct ttttttcctt ccaagaaaga
     7381 agcagcttgc aaaacagcat gtcctgttcc caattgttct gcttgtaacg catatttact
     7441 gcgttctcct agttgtgctt taacttgttc tgctccgtgt ccaacgatcg taatcacttc
     7501 atctggtttc gtttcactga cccgattaat gatatgttct accataggtt taccagaaac
     7561 gggatgtaaa actttatata attttgactt catgcgggta cctttccctg ccgccaaaat
     7621 aattgcataa cgtgcgtcca actgcgtcac tcctaaatat agtttgctga tttcagttta
     7681 tcccagtata cccatttttt caacaaaaca agtgattttc ttttatccca gcgttttctg
     7741 gataaaaaaa tagatagacc ataacgggcc tatctatttg gtaaccggtg ctctcctgcc
     7801 aactcctcgg tcacaagtca cactactcat aaaacatgaa aagctgtttt ttgaaatcgt
     7861 ttcttgtgac ttagagttaa acggtaggct tacaacctct tcattaaacg tttttcaatc
     7921 agctgacctt cggtattagc tcaataaacg gcaaaatatg aggagcaatt tttccgttta
     7981 ttgagctaat actcaggaca ggacgctgat ttcacaacct ctagataaac ggtgttcaaa
     8041 agccagtttc tcggtattaa gccaaattta tgaaaaaatg tggaaagcca ttttcacaaa
     8101 atttttctta ctactcgaaa ctaagcagct ttttcacaac ctcttattct gaaaaataat
     8161 ttcctggcac aaccgtaata tttttcgttt gagtatctac atctttgaca taaagcagtg
     8221 acgtataatc atcgatggcg cgatgaccgt tgaattttga ttcggcaaat acagtgatgc
     8281 caacaagttc tgattcgaat tcttcaatca agctcttcat tccgttgact gttccgccgc
     8341 ctttcatgaa atcatccact acaaggacac gtgaaccacg tttcaaactt ctcttagaca
     8401 gttccatctt ttcaattctt tcagaagatc cagacacata gttgacactt actgttgatc
     8461 cttctgttat ttttgagtcc cggcgtacaa taacgaatgg cgcattcaga taataggaca
     8521 cagcttgggc aataggtacg ccttttgtgg ctacagtcat tacagcatcg attttttcac
     8581 caaggtattt agaagcaatg attcttccaa cttgacgcag cagttcaggt tctcctaaca
     8641 aatcagagag ataaacatat cctcctggaa gcagacggtc ttgttctgat agacgttctg
     8701 ctagagattc aatatatttt ttcgcctctt catatgaaat ttcagggatg aacaatacac
     8761 cacctgcagc acccgggatt gtttccaagg ttccataccc tcgttcttta aatgttttct
     8821 tgatgattcc caaatcttca ctgatagaag atttagcaga ttcataacgt tcggcaaagc
     8881 ttgttaagga aatcaattca tgcgggtgat tcaataaata ctgtgtcatg tcaactagac
     8941 gttcactgcg acgtactttc acctattcca actcctttta agaaatttta tcgttttaca
     9001 ttatataagt ttttcttcaa aaattcgagc attttatgaa aaaataagaa aatagttcgg
     9061 tatttattaa aataaaaaga agcctataaa aaatttttcg tttttataag gcttcttttc
     9121 tttataattt agttattttt tcttctgaag gcaaccaata ttcgtttgat gatatttaca
     9181 atcaggaaca aagcaataaa gactaatgtg attgttgctc ctggaggtgt atctagataa
     9241 aacgagctag ttagtcctga cagcatccct agaaatccta cacaaacact gatagcaatc
     9301 acgacattga agcttttgcc taatcgcata ccgattgctg cgggaagaac catgattgcg
     9361 gatatcaaaa gtgcaccggc aatcgggatc atcacagcaa tagccacacc agtaatcaca
     9421 ttgaacatca tcgacatcaa atgaacgggt agcccatcca cgtgggcggt atcttcatca
     9481 aaagtcagaa catacatcgg ccgtttgaat aaaaagaaca atataccgat ggcaacgaat
     9541 aagattccta aaaagatgac ttgctcccat gtaatggtca cgatcgaacc aaacagatag
     9601 gattggatac ttgttgctga attccctccg ctcaaattca tcaatactaa cgctagcgcc
     9661 aaaccaccag ccattaaaat ggcaatggag atttctgaat aggttcggta gatcgttcgc
     9721 agatactcca atatcacagc tgctaataca acgactagca tggtcgtaag tgtaggatta
     9781 aggttcaaaa agaatcctaa cgctacccct gctaaagaga catgagaaag cgtatcagcc
     9841 atcagcgatt gtcttctgat cactaaaaac acacctagca tcggggcgat tccggcaatg
     9901 aagatagcgg caagaaacgc tcttctcatg aattcatatg aaagcaacgc cattgagaat
     9961 cctcctttct caccagtcgg atctgtctgt ccgcatactg tttgatatct tcatgatcat
    10021 gggtgatcat tagaactgat ttcccatgga catgcgcact atgtcggaga agacgataaa
    10081 attcatttct ggattcttca tccatacctg ttgttggttc atcaagaaca aataagtcag
    10141 ggtcagtagc aaaaatacgc gctaaactga ttcgctgctt ttgtccgcca gacaattcac
    10201 ctattcgccg atttcgcata tcccacatcc ctacagcttc taaagcacgc tgtacatgtt
    10261 gatgatcttt ttcagagaga cgcttgaacc agcggcctct tggatatctt cctgaacgga
    10321 ctaattcgat caccgtacta ggaaaacctg cattgaaaga tgcaacttgt tgaggaatat
    10381 aaccgatact taattttttt ccttcttgat ttttctcagc aatctttatc attccttttg
    10441 aaggtttcag taatcctaac gatgctttca ccaaggttga cttggctgct ccattttctc
    10501 cagttaaaat gacaaattca cccgcatcta catgataaga gacatcttct agtacaggtt
    10561 catcatcata gtaaaaggtc aaatcagata tttcgatata acgcacctta caacctccat
    10621 tctaaaactt aattgatact ttcttttaaa gctttcagat ttttttccat gacagaaata
    10681 taattttcgc ctgctttgat ttgttcatta gtcaagcttt ctaaaggatt caacacttct
    10741 agcttcactc ctgtttcatt cgacaatgtc tcagctacct tcgaagaggc attttcttca
    10801 aaataaatca ctttgatgtt gtttttcttc acaaattctt ttagctcagc taagcgactt
    10861 ggtgtgggtt cctgatctgg tgaaacgccc gaaatcgcta cttgttttaa gtcgtattga
    10921 ttcgctaaat aagcaaaagc agcatgctga gtgacaaatg tgcggtttgt tgcatctttt
    10981 agctcatcgg agtatttttg atccagtgct tcaagctttt tgatataagc atcggcattc
    11041 ttcgtaaaag tttccttttt atctggatat ttttcgccta attcttcagc aatcgttctg
    11101 acttctttta tagctaatac cggatcgagc caaacatgtg gatcgtattc atgtgaatga
    11161 ccatgccctt cttctgattc ttcatgttcg tctgcttctt gcgattctaa acggtcgatt
    11221 cctttagctg cttcgatcat caaggtctgt ttgctatcca ctgaatcctt taacgaatcc
    11281 acaaaaaatt ccatgtctga actattatac acgaaaacat ctgcatcgga aatttctgct
    11341 ctttcttttg cacttggttc gaaatcatgc ggctccgttc cagcagggat caaaagtttc
    11401 acgtttcctt catcccctac gatctctttt gtaaaatcat acatcggata aaaagtcgta
    11461 acgatcgtca aatcctcttt tttgtctgct tccttgttcg tatttccaca tgctgcaaac
    11521 agtaaagcac ctatcattac cgtagctgtc aaaagatatt ttttcattta tatgctcctt
    11581 tcaatgaaca aatcggaatt ttacgattta ttaacgtgaa ctaatgtatc aaaataaaaa
    11641 aacacagtca atagaaatcc atcaatttct gatttaagca tgtatattct tgattaaatc
    11701 gtaaaatgga tgagttgctg ctcatatcta gccatttcac tacaataaaa aactgaagaa
    11761 taccatttta ggattcctca gtttttcatc acctatttta cttcttatat tttattgcag
    11821 ggttcgtact aaataaactt cctcacaaaa accttttaac ccattatata ctctctgcgc
    11881 tcttgaataa tgttgacaca aggcaaaaac ggttggtccg cttccactca tcaatgcagc
    11941 atctgctccg tacttgatca tacgctcttt gatttgatgg atgactggat gccgctttcc
    12001 cgttatatct tctaaactgt ttcccatttt tttgatcatc tgctgataat cttgctctcg
    12061 gatggcttca gcaagtccgt ggatgtcttg atgttggatg gcttgcgtat ctacttctct
    12121 aaacactgta cgtgtagaga cactcattct tggtttgacc aacacaaccc agcactgtgg
    12181 catagaaggt aatacttgga tttcttcccc tttgccaccg acaaatgccg tacttccgta
    12241 gacacagtaa ggaacatctg taccgacttc caaaccgatt tctgccagtt cttctaacga
    12301 acagcccaga ttccataatt ggttgatccc tcttagagca gctgcacagt ctgtacttcc
    12361 tccgcctaat ccagctgcta ctggaatatt cttcttaaca aagatcttga caccggtttt
    12421 gatattgtat ttatttttta gcaataaagc agcttgataa acattgttcc gttcatctac
    12481 cggtaaaaat gctttatttg tttctacaat gattttgtct tctgttattt cttcaaaaaa
    12541 tagatgatct gccaagtcta cgctcgacat caccatttcc aactcatgat acccatcttg
    12601 ccgtctatat aatacatcca gtcctaaatt tatcttggct ggcgcttttt caatcaattc
    12661 catcctctca cccattcgaa aagtctaagt cgcaagtatt ctatcatagg gaaaaataaa
    12721 agtatagctt atgtctgcct agtggttgat tttgtcatcg tttcaacagc tttcacagaa
    12781 ttttctcttc ataatccaaa caagttctag tgattcttat aattctgtga gcacttcttc
    12841 tgccaaactc ggaacatttc ctttttcttt tcctatcaga ctgagtagct gcatggtagc
    12901 aaatccgaaa ttacttcctt tcctataagc aaaataacgt ccttcccaat gatcgacata
    12961 ttttaatttc ttttcataag cagactggaa ggaatagaaa aaattaccat aattatatac
    13021 gatatgcatg actttttctt gaaagaaaga aaaatctgtt tctccaacat tagctaatgg
    13081 agctaagcct agattggctt ccgagtagcc cattttctcg tatacaataa acagattggc
    13141 taacagatag tcagctaatt cctcagattc tttaggtaag acccgcaaca aatcatatcc
    13201 cgcctgcttc ttcgtcatcg gctgagcggt gatgaagcca acgacttttt gatctttacg
    13261 gacaataccg acaccgctag ataatacata tgatttatca aatcgtccca cagagaagta
    13321 tttttctcgt gcaccttgaa gccatttttg tgaaacttca tttacagctt gatgtaattc
    13381 ttccggcagt tgatcgtacc aagtaaaatg gtatccttct ttcatcaaac gttgatattc
    13441 aagttgctga ctcagccgag acaagtcttc tgttgctaga tcaacaatcc ctgcttcacc
    13501 tactttcgtg aagtgaaaac ccaattcatg aagcatcact acgtagcttt ctgatatctt
    13561 ataaaacgca agctgatagc ctaaacaatc tgcttgtttc ataaactgat tcgttgctgc
    13621 ccgcatcatt ttaggattgc caactggatc tcctaaaaca aagcatttgt ttcctttgat
    13681 ctgataacca aaaacgactt gatcttggtc gtcttcttgg taataaaaat aactgtaacc
    13741 tgacaagcga agctgatggc ttgtagctgt gcctccgtat gtttccaata aacggtcaaa
    13801 acggtctttt tcgtactcac tgccaagctt cgtagtatct tcagctaaat attgataaag
    13861 cgccactaaa gctagcaatg agatgcccaa accaataagc cctgagaacc acacatcatc
    13921 tgaaggaaac aaaataaaat gatttcctgt tattcgaccg actaaaaagc gtccgctgtg
    13981 atagcctgca attgcgtaaa cgataaacaa aaagccgaac aagctagcat caaagattat
    14041 agctccccaa gaataaacga attttgttcg ataaaattct tttctggata accacacagc
    14101 taacagaatc aagacatata ccaatatcaa ctgccaagat aacgtacgtg tgattgtatt
    14161 taaaatacca aaagatagta aagcaatcgt aggcagaaat gcctttttga cttttactga
    14221 aagtgctcta gcaaggccga gtaataaaaa gccaaccaat agattcactg tctgatctaa
    14281 aaaattgaat gagaacggca ataatacttg aaatagccga ctgattgcag acagattagt
    14341 gattgttgat agaagtacca tcatgatccc cgcaaaatag acagcactta cgagcacgat
    14401 atgcgcaacc ttttggaaaa acagtcgcgg aatgttatct aaaaatcgat tgattttgac
    14461 tcctgtatgt gtgatgaaca atagaagtcc cgtcataaaa ggtagaacat aataaaacaa
    14521 ccgataatat aatagccaaa ctactgctac atcttgccga acaggcaatt gactcattcc
    14581 taagatcatc aaaacgtcaa aagtccccat tccacctggg accatcgtca gcattcctat
    14641 caatgtagca atcatgaaca taggatagat cgaaagcatc gaaatatcta ttctcatcaa
    14701 cttgccgatg atcaaaaata ccgcaagggc tcccgtccat tgtccaaagg atgccagtag
    14761 cagaccaagc aagccttttg gataaaactc gaagaacaat gtctttctct ttagataagc
    14821 aaaaaagaaa atgactggtg ctaaaaaact accgccgaaa agccagatcc aatagcttcg
    14881 aaaaatactt tctgactgaa taaaaaagac atcaataaat gtaagaaacg accatacaga
    14941 caaaccagtc agcataaata gtgcgacttt tgatacagtg gctaaaacca tttttctatt
    15001 tgtctgcttt ccataaaaac tagcccgcag acttgctcct accacaccac caaaaccggc
    15061 gagattgttg atcgtgtttg ttacccacgc agcaagaaga aactcttttc tttccatttt
    15121 tggttttcct tgtttttcta agactctgat cgtcacccaa tcatacaaca acatcggagt
    15181 gacacccagc agtccagcca gaatcatgag caggatcgtc atgcggcttt gttgtcccat
    15241 cctttgccaa atatcttgcc aactcatgcc attagcaata tttgcgactt gatttgccac
    15301 aaagactaac acggagccga aaaaaatcag ttttaaaatc agactatgtt cctttatcca
    15361 ttgcagcatc aagtatcgtt tatttttcac attttcccat tcctaacttg tatacttttg
    15421 tgtcataccc caatgttatc ttatttattt taatttttcc actttagacc tgttctttgc
    15481 ttgcgatgat agaaaaagaa agatatccta atgctaaagg agaggataac atgaaagtag
    15541 gatttatcgg cacagggaat atggcacaag ctattattga tggtttatta aaaaaaggaa
    15601 cagtctcgaa agaagaggtg ttcgtttcca gtggacattt tgaaaatgca caaaaatttg
    15661 ctgctgaaac tggcgtccat gcatgcaaaa gtaatcagga agttgcatca caatcagata
    15721 tcgttctttt agccgtgaaa ccaaaaatca ttgaaaaagt attggaagaa ctaagagaat
    15781 atagccagaa agtcttattc atttctattg cttcaggaaa atcattaaca gatttagaaa
    15841 cagcgcttga agatcctcag gcctccatcg tacgtgtgat gccgaatgtc aatgtcagcg
    15901 taggagcagg cgtttctgcg atttgcaaaa atgaagctgc cacggaggaa cagcttcagc
    15961 aagctacgat gatttttgaa gcaatcggca gcgtctatcc tttagcagaa tcagatttca
    16021 gtacattcat tggccttgct ggcagctcac ccgcattcgt ttacatgctg atcgatgcga
    16081 tgggacggac tggtgtactg aatggtttgt ccaaacagac agccactgag attgctgcaa
    16141 aagcaattat gggttcttgt caaaaattcc tttcaagtga taaaaatcca tgggaacttg
    16201 tagaccaagt ttcttctcct ggcggtacaa ctgttgcagg tgtagttgcc ttagaagaag
    16261 caggattcat cccagcaatc atcaaaggaa tcaatacgac gatcgagaaa gatcttgaga
    16321 tgttgaataa aaaata
//
LOCUS       DAMEET010000063        16271 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.064, whole genome shotgun sequence.
ACCESSION   DAMEET010000063 DAMEET010000000
VERSION     DAMEET010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.064"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..347
                     /locus_tag="PB780_001848"
     CDS             <1..347
                     /locus_tag="PB780_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDE0733900.1"
                     /translation="LEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLKEYKIFQSMS
                     RKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNERIKQKLNWQS
                     LVQFRKTTVTVV"
     gene            536..1204
                     /locus_tag="PB780_001849"
     CDS             536..1204
                     /locus_tag="PB780_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDE0733901.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1404..1634)
                     /locus_tag="PB780_001850"
     CDS             complement(1404..1634)
                     /locus_tag="PB780_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733902.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1761..3143)
                     /locus_tag="PB780_001851"
     CDS             complement(1761..3143)
                     /locus_tag="PB780_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDE0733903.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(3299..4486)
                     /locus_tag="PB780_001852"
     CDS             complement(3299..4486)
                     /locus_tag="PB780_001852"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDE0733904.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4493..5524)
                     /locus_tag="PB780_001853"
     CDS             complement(4493..5524)
                     /locus_tag="PB780_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDE0733905.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5540..6994)
                     /locus_tag="PB780_001854"
     CDS             complement(5540..6994)
                     /locus_tag="PB780_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDE0733906.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            7169..8074
                     /locus_tag="PB780_001855"
     CDS             7169..8074
                     /locus_tag="PB780_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDE0733907.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            8165..8842
                     /locus_tag="PB780_001856"
     CDS             8165..8842
                     /locus_tag="PB780_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDE0733908.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8839..9009
                     /locus_tag="PB780_001857"
     CDS             8839..9009
                     /locus_tag="PB780_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733909.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            9123..10019
                     /locus_tag="PB780_001858"
     CDS             9123..10019
                     /locus_tag="PB780_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDE0733910.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(10074..11444)
                     /locus_tag="PB780_001859"
     CDS             complement(10074..11444)
                     /locus_tag="PB780_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDE0733911.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11682..12428
                     /locus_tag="PB780_001860"
     CDS             11682..12428
                     /locus_tag="PB780_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDE0733912.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12425..13264
                     /locus_tag="PB780_001861"
     CDS             12425..13264
                     /locus_tag="PB780_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDE0733913.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            13265..14215
                     /locus_tag="PB780_001862"
     CDS             13265..14215
                     /locus_tag="PB780_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDE0733914.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14356..15108
                     /locus_tag="PB780_001863"
     CDS             14356..15108
                     /locus_tag="PB780_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733915.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            15127..>16271
                     /gene="queG"
                     /locus_tag="PB780_001864"
     CDS             15127..>16271
                     /gene="queG"
                     /locus_tag="PB780_001864"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDE0733916.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRK"
BASE COUNT         5284 a         3045 c         3279 g         4663 t
ORIGIN      
        1 gtttggagga agctatccag accactaacg attgtcccta tcgtcgtacg tttcactcag
       61 atcaaggttg ggcgtatcaa atgaaagctt atagaaacaa attgaaggag tacaaaatct
      121 ttcaaagcat gtctcggaaa gggaactgct tagataattc acttatggag aatttttttg
      181 gcttattaaa acaagagatt tttcacggga aagtttataa ctgtttcgag gaattaaaat
      241 cagccatcga tagctatatc tattactaca ataatgaacg gataaaacag aaattaaact
      301 ggcaaagtct cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta
      361 gagcataaaa aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac
      421 tacagtgtca cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc
      481 gtgttcgtga gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat
      541 tttttcaatt acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt
      601 gcttgggaag aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt
      661 aggtgggatc ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt
      721 tgccattgcg ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga
      781 aaaggtcaat cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa
      841 catgacagag ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca
      901 gcaagaatgc ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat
      961 gagtgtattg aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg
     1021 gcaaattcag cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa
     1081 cttgaagaaa gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga
     1141 aaaagggctc tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg
     1201 atagatagac agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt
     1261 tttcacaacc tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag
     1321 ccaaaattcc acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag
     1381 ttgatcactt ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca
     1441 atataccgta cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag
     1501 gcttcaccaa tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa
     1561 ttgagctgaa tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt
     1621 tcttgcaatg tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt
     1681 tgataccgtt gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc
     1741 gatctcgata ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct
     1801 tctaatccac tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt
     1861 cctccttgca agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc
     1921 gtatcgacaa caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg
     1981 ttaagctgta aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata
     2041 tcctggtagt ggtaatgctg gtggaaaatg gtcaataaag catctgctag atctctgcta
     2101 gcatttgtcg cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga
     2161 tagcctaaag agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc
     2221 aagcgagtgc cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt
     2281 gtataatctc tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct
     2341 tgtcccaaaa aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc
     2401 cctagcttgc ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata
     2461 cccatttgtt tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct
     2521 gagattttcc ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt
     2581 ccgtcttttg agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct
     2641 atagtcgtgt agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg
     2701 tacgcatttg ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg
     2761 acggaataga catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct
     2821 ctgttctttt ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag
     2881 gggaagggaa gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca
     2941 ggactggaag ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac
     3001 atgactacta atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg
     3061 taaaaagatt tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat
     3121 accggatttt gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc
     3181 tctttccgtg tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga
     3241 aattgctgag aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc
     3301 aaacatgaac agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa
     3361 attccggagc atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag
     3421 gtatattttc ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc
     3481 gtgcacctaa gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc
     3541 cagttacttc ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg
     3601 cttctaagtt cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa
     3661 cggcatggcg agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa
     3721 attcttcttc atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca
     3781 aggcttcttc gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct
     3841 tattcgtatt catgatgacg attgcataac catctagttt cactggaacc atttcgtatt
     3901 tcagcgtatt tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact
     3961 gatccatgat acctgaattg acaccgatga attcattttc tactttttta cctgtttgga
     4021 ctagttccaa gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata
     4081 attctaatga agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata
     4141 actcaaaccc atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta
     4201 cgtaatttgc ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga
     4261 caccgatatc gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg
     4321 ctacaccttg cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg
     4381 tgtgttcacc aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc
     4441 cgaatgtttc ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct
     4501 ttcttgtttt gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa
     4561 tcgaaccaaa tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc
     4621 caccgtgatg tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag
     4681 tgaaaataac cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc
     4741 tagtcaagcg agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga
     4801 ttccgtcaac aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag
     4861 gtatttgaaa atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat
     4921 ctgttcgcaa aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact
     4981 gagaagcatc tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt
     5041 ggtcactggt gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga
     5101 tttccacatc cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa
     5161 tgacagaagc ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc
     5221 caggagcacc accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt
     5281 ttccattgag agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac
     5341 cgatatacgg atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc
     5401 gatcatttga ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct
     5461 tgtttttgtt ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct
     5521 tcattttcaa gatcctttct taatgggaag gaactactgc atcgtgaagt tcatcttcca
     5581 ttgtatcagc tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct
     5641 ctgtcaatgt aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag
     5701 catagaatgc ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag
     5761 cattaccagt catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac
     5821 cagcgatttt atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat
     5881 ttttccattg accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca
     5941 attgttgagg gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg
     6001 cgatcgtaaa gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc
     6061 gacgagagat gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg
     6121 cagcaactag accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt
     6181 agaataatac ggcagttgtt gctacattgg caatcgcata agcgatataa gcaagggcaa
     6241 gccacatcaa ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt
     6301 cattttgctg acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag
     6361 taccgcctga aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa
     6421 tatgtttatg agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag
     6481 taccatttgc acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct
     6541 ttgtttccgt actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa
     6601 aactatccaa taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag
     6661 tagctaagcc aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc
     6721 cgataactaa ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac
     6781 caatcaaagg atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg
     6841 tagcaatcat ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga
     6901 ataccataaa ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag
     6961 cgtaggacac acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata
     7021 tataatacta agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta
     7081 acaagaaatc gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat
     7141 tatactaaag gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca
     7201 tcaacaagta gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt
     7261 tcattctttt ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa
     7321 aggatcttat catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat
     7381 tcgtccagga gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg
     7441 gctttcattt ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga
     7501 ccaatacacg atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat
     7561 gaaagatacg gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt
     7621 tttatcagtc cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc
     7681 ctctttagct gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt
     7741 tgaggaaatc gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa
     7801 tcatatgggt accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt
     7861 tcttctttct ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag
     7921 tctagtcgta tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg
     7981 taaacgtatg aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt
     8041 ggaaaaacaa accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc
     8101 ggcaaaagaa acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa
     8161 agggatgcgt acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca
     8221 atttttcaaa gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta
     8281 tccagacgga aaaatagata tagagactga actacagctc ttggcccagc atgatcggat
     8341 cattttccag tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga
     8401 taatgtcttg gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac
     8461 aacaggtgtg gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga
     8521 atttttgagg ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt
     8581 cgttttagca caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta
     8641 tcagcagtat ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat
     8701 tcaaagagta caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat
     8761 cgtagaagca ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga
     8821 aatgaaagga acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc
     8881 gaaaaatcag aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag
     8941 atagacgaac gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc
     9001 aactggtgaa attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg
     9061 ctacaataag aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa
     9121 aaatgtatga tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa
     9181 aacaggcagc tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca
     9241 tagcaggagg agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta
     9301 cgaccataac ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa
     9361 ctaaaacaaa agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac
     9421 ttcaagcaat cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg
     9481 aagaatacca gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg
     9541 ctaaccaaaa agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg
     9601 cagcgtatca ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat
     9661 tatcggctaa ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg
     9721 cctctcttca agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag
     9781 cagagtcggt agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct
     9841 ctgaaactaa taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag
     9901 aaggtccaaa tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga
     9961 atgggatgag catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat
    10021 aaaagcaata aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt
    10081 ccggttcttt tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca
    10141 ataatcaatg agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa
    10201 aaagcaagtg tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc
    10261 attttatctg cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga
    10321 accataccag gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg
    10381 atgaacattg gtagaagata atttaagatc agaccaatta agattccacc tgagattgct
    10441 acgacaggat aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt
    10501 ccatttatgg caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct
    10561 gataatgccg ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact
    10621 ttagaaaaag tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata
    10681 ctcacaatga taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg
    10741 acagcttcaa tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt
    10801 tcgattcctt gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct
    10861 ccagtgaaga atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta
    10921 ccaaatccaa ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact
    10981 ttgatgatcg aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc
    11041 agcataagaa acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa
    11101 taattcatat aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa
    11161 atattactcc aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt
    11221 gcaaaagaac cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa
    11281 tacaaaaaga atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg
    11341 gttgaggagg cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg
    11401 atatgacggg cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg
    11461 ttgttgagtc aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt
    11521 tagccttatt gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat
    11581 tcgaaaaaaa ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat
    11641 ttaaataaaa agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg
    11701 taattcaaga cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga
    11761 cgataaatcc acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat
    11821 ttatgaaatc tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac
    11881 agtcggtaaa ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc
    11941 tttcctttcc aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa
    12001 ttggacaaag acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag
    12061 ggatggaaga atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag
    12121 tttttattgc aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg
    12181 tagggattga tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt
    12241 caggaaaaac gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg
    12301 aggttattct tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat
    12361 cgaagaacct tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga
    12421 aaaaatgatc caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt
    12481 ggtcacttcc atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg
    12541 agggatgtct ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc
    12601 ttatatgttt ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc
    12661 agtgattggt ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt
    12721 ctttagctcc ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga
    12781 tttgtatcat attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac
    12841 agcactcgtt agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat
    12901 cacatcattc gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta
    12961 tcttttgatg ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat
    13021 cttggtgatt gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt
    13081 actgaaaatg atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt
    13141 tttcagttat agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt
    13201 ttttatcctt gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag
    13261 atagatgaaa acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc
    13321 cggttgcgga aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt
    13381 cgtggctacc aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga
    13441 tatccacagt atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga
    13501 catcaaaaag gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg
    13561 taacagctgg ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt
    13621 tgcagttagc aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc
    13681 agatccccat gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg
    13741 tatattctct gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg cagaagcata
    13801 tgtggaaaaa ctaaaagaat tagatacaca agcgaaggaa agttttgctt ctatcgaaga
    13861 gaacaaaaaa ttattagtaa caagtgaagg ggcattcaag tatttttcac gagcatatga
    13921 tttgccagca gcttatatat gggagatcaa tacagaaagt caaggtacgc ctgatcaaat
    13981 gaaagcgatc attgatcaga taagagcaaa agaagtaccg gttttattcg tggaaaccag
    14041 tgtagactca agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa tctatgataa
    14101 actgttcact gattccatag caaaagaagg agaacaagga gattcttact atcagatgat
    14161 gaaatggaat attgaaacga tccatgaagg tttgtcacaa acaaaagaaa gctgaaaagt
    14221 gcaaggtcta gcttgaggag cagaccttgc attttttata agagatatga aaaaaaatta
    14281 tgctgttttt cgtcgtacag ataccttttt tgaagaaatt ccgttatagt tagagtaagg
    14341 aaggaaggtg tggctatgaa ttttacacgt aaagataaat atacgattaa agaagaagtc
    14401 tttcgattga tcagccagca ggaaaatacc gacaaggatc aagaccttca atttcttgaa
    14461 gcagaattag ctaaattatt catagcaaat caaaatgaga caacagaacc agatgaagag
    14521 ctgtacatag gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa taccggtatc
    14581 ggttattttg tgttcctttc tccagtcggc ttcacacctg gcgtattagt taaacaaaac
    14641 gacgaaataa ccgtcaaagc atggcctgca ttattttcag tcatcaaagc agaaggagta
    14701 atcaaaaaga tccagtcggc ctattataaa agtcaattta ttgccaacag caaattaatg
    14761 aatatgtttt ataatcgtct agtagaaatt gctaggcctg ggaaaatccc gcaaacattc
    14821 acgtacacac agcaaaaaaa tctctatgaa gaagtattgt attcccgcga tctgtctgag
    14881 ttttcgccat gggaagaggt aaggctccat gaactgttaa gtacaaaacc agtttcattg
    14941 aattttttta atgcattaga taacaaaaga aaattaattt caacaaatta ttgggaagta
    15001 tttgctgcaa gaaaaaaatg gatggaagaa aagaagattg ccgatcagca gatccagttt
    15061 gtccagctga aaaaagaaaa actatattat gggttataca gccgttaaca gcaagcgagg
    15121 aagaccatga cattaaaaga agaaatcata caagaaagca aacgattagg aattgacaaa
    15181 atcgggttca ccacggcgga accttttgat tcattaaaag aatcgttgga agaacaaaaa
    15241 gcagctggcc atacttctgg ctttgaacat ccaaatatcg atgagcgtct ttatccagaa
    15301 atgacatttg atcagccgaa atcgatcatt tccattgcac ttgcgtatcc tactaggatc
    15361 catgaagaag tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc ttgggggatc
    15421 gattaccatg atatactcag agaccgtttg aaccgattga tcgaattcat ccaaacaaga
    15481 gcagaaaaat ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga tactggagaa
    15541 ctgatcgatg tcgctgtagc acagcgagct ggtttaggat ttattggacg aaacggattg
    15601 ttgatcactg aagagttcgg ttcatttgtc tatcttggtg agatcgttac taatattgta
    15661 tttgagcctg ataaaccagg agaatttggt tgcggtgact gtatgcgctg cgtgactgct
    15721 tgtccgacaa aagcattgct tggtgacgga agaatgaatg cccagcgatg tttgtcttat
    15781 caaactcaga caaaagggat gatgccggaa gaatatcgta aaaaaatgcg aaatgtcatt
    15841 tatggatgtg atatctgcca gttagtctgc ccttacaatc aaggaaaaga ttttcatttt
    15901 catgaagaga tggaaccaaa agtagacgaa atatatccaa aattaaagcc aatgctttcg
    15961 atgtctaata aagacttcaa acagcaattt gggcatttag ctggttcatg gcgtgggaag
    16021 aaacctttgc agagaaacgc attgattgct ttagctaatc taggtgatcg aacggcattg
    16081 cctgagattt cagaatgttt aacagatgtc cgccctgtca tcagaggaac agcagcttgg
    16141 gctattggac gtctaggaac aaaagaaccg gaaaaatggc ttgatgtatt agatgaaata
    16201 ttagctaaag aaacagaaga agtcgttata aacgaattgg aacatgccca gaaattattg
    16261 cggagaaagt t
//
LOCUS       DAMEET010000064        15638 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.065, whole genome shotgun sequence.
ACCESSION   DAMEET010000064 DAMEET010000000
VERSION     DAMEET010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.065"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(12..482)
                     /locus_tag="PB780_001865"
     CDS             complement(12..482)
                     /locus_tag="PB780_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDE0733917.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(641..1099)
                     /locus_tag="PB780_001866"
     CDS             complement(641..1099)
                     /locus_tag="PB780_001866"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDE0733918.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1270..2415)
                     /locus_tag="PB780_001867"
     CDS             complement(1270..2415)
                     /locus_tag="PB780_001867"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HDE0733919.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2428..3345)
                     /locus_tag="PB780_001868"
     CDS             complement(2428..3345)
                     /locus_tag="PB780_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HDE0733920.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3773..4336
                     /gene="efp"
                     /locus_tag="PB780_001869"
     CDS             3773..4336
                     /gene="efp"
                     /locus_tag="PB780_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDE0733921.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4437..4607
                     /locus_tag="PB780_001870"
     CDS             4437..4607
                     /locus_tag="PB780_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733922.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            4822..6081
                     /gene="lysA"
                     /locus_tag="PB780_001871"
     CDS             4822..6081
                     /gene="lysA"
                     /locus_tag="PB780_001871"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDE0733923.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6068..6868
                     /gene="dapF"
                     /locus_tag="PB780_001872"
     CDS             6068..6868
                     /gene="dapF"
                     /locus_tag="PB780_001872"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDE0733924.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            6871..7752
                     /locus_tag="PB780_001873"
     CDS             6871..7752
                     /locus_tag="PB780_001873"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDE0733925.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7749..8465
                     /locus_tag="PB780_001874"
     CDS             7749..8465
                     /locus_tag="PB780_001874"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDE0733926.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8471..9166
                     /gene="dapD"
                     /locus_tag="PB780_001875"
     CDS             8471..9166
                     /gene="dapD"
                     /locus_tag="PB780_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HDE0733927.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9182..10183
                     /locus_tag="PB780_001876"
     CDS             9182..10183
                     /locus_tag="PB780_001876"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDE0733928.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10180..11541
                     /locus_tag="PB780_001877"
     CDS             10180..11541
                     /locus_tag="PB780_001877"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDE0733929.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11501..12652
                     /locus_tag="PB780_001878"
     CDS             11501..12652
                     /locus_tag="PB780_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDE0733930.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12627..13796
                     /locus_tag="PB780_001879"
     CDS             12627..13796
                     /locus_tag="PB780_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDE0733931.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            13971..15350
                     /locus_tag="PB780_001880"
     CDS             13971..15350
                     /locus_tag="PB780_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDE0733932.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
BASE COUNT         5101 a         2760 c         3327 g         4450 t
ORIGIN      
        1 tttttattaa gttaaagaac ggattcaatt gcagcggtca ttttcaatgg agaagtttgt
       61 ggtgcaaatc gttcgattac tttgccattt ctatcaatca aaaatttagt aaaattccat
      121 tttattgctt tagtgacagt accaggagct tcatttttaa gataagtaaa aagaggatct
      181 gtattttctc catttacgtc aatcaattga tgcataggga aggtaacgcc ataagtggtt
      241 ttacaagctt ctgctgcttc agtcgctgaa tctagctctt gtttaaattg attggaagga
      301 aagcctaaga taaccagacc ttgtgtctga taagcttggt aaagttcttc taattgttta
      361 aattgaggtg ctagaccgca ttttgttgca gtgttaacaa tgattatcgg tttgcctgca
      421 tattttttca atgaatagac agtgccgtct tctaaagtag tttcaaaatt ataaatagac
      481 ataaagtttt ctcctttcta ttttatataa taaaaagagc gctaaactga aaactagcta
      541 tttgttttcc cgtttagaac tcttttcaga caataaatat ttaataaaat acgaagataa
      601 gatagctata ttgcttctca tctttacgtt cttttagaaa ctatgcttct acttcagacc
      661 attcatcgcg ttttgctaaa aattcacgag ccaaagcttg tgcaccttct agtgtatggc
      721 tggcagccca gccacattgt gtttcattgc ttgcaggtac ttcagtagct tcaagaacat
      781 ctttcattgt tgcttcaaga acatctaaaa tgtcttcgta gctttcatga ttcaatacag
      841 tcaagtaaaa gcctgtttgg catcccattg gagaccagtc tacgatgtaa tctgcgtgat
      901 tgcggattag ttcggcagtc aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt
      961 ctttatttgg ttgtttaaag cggacgtcgt atttaatgat gacatcgcca tgcgcacccg
     1021 tttttcgatc agctacacgt acataaggtg ctttgacttt tgtatggtca agattaaaac
     1081 tttcgacatt cattctcatt tgtaagactc ctatctgttt gttcattcgt tttgatgctt
     1141 ttcactaaag aattatttag tgaaatttac aaaaacgtct ttctacttga ttctaacact
     1201 tatcgtttcc aaggtgtagc aatctgaaat attttttaga tttttctgct aagtcgttta
     1261 ttgggacttc tagttcttga ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg
     1321 accatactca attcctacag aaagacggat caagccgtcc tttatgccag cctcttctcg
     1381 tttttctttt ggaatagacg catgagtcat gacagaagga atttccacta aactttctac
     1441 gccacctaaa ctttctgcta aagtaaatag ttggagtgat tcaacaaatg gaatagcaga
     1501 ttcttcattt tttaaagtaa aagaaatcat tccgctaaag ccgctcatct gctgcttagc
     1561 aactccatgg agttcatgat cgggtaatcc tggatagtag accttttcta ctgctggatg
     1621 agaaaataga aaatcagcta ccacaaaagc atttttttga tgttcctcca tacgaacact
     1681 tagtgtcttg attccgcgct gaagtaacca gctatcttgg ggaccaagta cggctccgat
     1741 agcattttgg taaaatccga tctgatcggc tagatcttta tggttagaag tcactaaacc
     1801 agctactaca tcgctgtgtc cgcctagata tttggtaccg ctatggacaa cgatatcaga
     1861 accgagatct aaaggtcttt gaaaataagg agtagcaaat gtattgtcag caataactat
     1921 caatccatgt tgatgagcca gagtggcact tttttctaaa tcagtaatct ttagaagcgg
     1981 attgctaggt gtttcaagat aaagtgcttt tgtattgggt ttgatggatt gttctatttt
     2041 atcaagatta ctcgtatcta tgatcgtgta ctcgagacca tttttagtaa gaactttgtc
     2101 aaatagacgg aatgttcctc catagacatc atctcctaat aagatatgat cgccagcttg
     2161 aaaaagagaa aaaacggcat gaatcccaga aagaccagaa gaaaaagcga atcctcgaac
     2221 accgccttcc aaatcagcaa tcagttcttc caatgcaaaa cgagtaggat taccagatct
     2281 tgaatattca tattgtttag gctggccgac gccatcttgc cgataagtgg atgtttgata
     2341 aataggtaca ctgacagctc ctgttgtggg atcttcactg atgcctccgt ggatcaattt
     2401 tgtttgaatg tgcatgtttc tttcctccta taagtaaatg ttttttgata aatagcggtc
     2461 tgctgcgtct ggaaaaatcg taacgacacg atgaccaggt ggtaaacgac gaatttctct
     2521 taatgctgct gcaaaagccg caccactcga acttccaact aatagtcctt gttctcgtgc
     2581 taactgccta gtatagttaa acccttctac atcagagatt gtctcgatct gattgattga
     2641 aaggggagaa agaaacggcg gaatgaattc tacaccgata ccctcaattt cgtgaggggc
     2701 tggatcacca ccatttaaca cagagccctc tggttctaca ccgattattc gaatgtcggg
     2761 atatttttct ttcaaaaagg tagaagttcc agcaaaggtt ccaccactcc caattcctgc
     2821 tacaaaacta tggatatttt ctttgatttc atgaaagatt tctggcccta acgtatgata
     2881 gtaagcagca ggattatctt tattttcaaa ttgaagaggc agatagctgt ttgatatctc
     2941 ctctgcaagt tttttacttt tgctgatggc accgatgatt ccttgctcac taggagaatg
     3001 gatgatcttt gctcccaatg ctttcatcaa ttgttgtttt tctaagctaa acttctctgg
     3061 aacaacaaaa accgttctta atttgtatgt gagtgcggcc agagcgatcc caattcctgt
     3121 atttccggca gttggttcga tgatcgtcgt tttttcattg atttttcctt gggcaaatcc
     3181 ttctttgatc aaataactgc ccaatctatc tttgatactt cctccaggat ttaaatattc
     3241 tagttttgca taaatagcag aacctttagg catagaatag tgctgattgt caaagcgata
     3301 aagcggtgtg gaaccaatag cttctgtaat tgtcgagata atcatgtggg cctcctaaaa
     3361 aaagataata aaaaaaggag ctgactatta aatcagctcc ttgaaagaca tagagaaaaa
     3421 ttgagtagat aaaaagataa gttacctggt actcattcta tgaagggcaa ttaatagaca
     3481 tgacaaatag tatgctgctt ttatgcacca tacaacagca acgattcaat tgtttttggt
     3541 tgctgactgt catgtgtatc cctccaagta aaatttttat taacgatagc atgaaaaaaa
     3601 ataaaagtca aaggcttgaa agaaaaaaat atttcgatag catcgtttac ccgtgtttat
     3661 ttttctgaca aataggcatg agaccttaaa agatagagaa tactctagtt ttctggccgg
     3721 ttgtctgata caataaagtt gattatgata aagaaaagaa ggagcaaaaa taatgatctc
     3781 agcaagtgat ttaagagccg gtatgacttt cgtacaagac ggcaaactga ttaaagttct
     3841 ggatgcaagc caccataaac ctggtaaagg aaatacagtt atgcgtatga aactgaaaga
     3901 tgtccgttca ggtgcaacat acgacacaac gttcagacca gaagaaaaat ttgaaaaagc
     3961 acatatcgac acaaaacctg tacaatattt gtacacgatg gatgatacag ctttcttcat
     4021 ggatttggaa acatatgagc aatatgaaat tcctgtagaa acagtagaag aagaaatgaa
     4081 atatatccta gaaaacatgg aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt
     4141 tcaattacca acaactgtcg tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc
     4201 aacagtcact ggttcaggta agccagcgac aatggaaact ggcttagtag tcaatgttcc
     4261 tgacttcatc gaaacagatg aattgttgga aatcaataca gcagaaggtt cttatgtaaa
     4321 acgtgcgtca aaataattcg ctaacggatt gataagaatt tgaaaacaga gcttgacctc
     4381 ttgaaaggca gatcatcgga tctgcctttc aagaggtttt ttgtggaatc agcaaattga
     4441 aaaataaaaa agtatttgat tttatttata ttttgaaaga tgaatggcaa aagctagaga
     4501 aagaaaaaac tgcacttatt tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc
     4561 gaactattcc gaactatatt gaagcgaaaa tattttttca tcactaaata gtgttgaatt
     4621 tatgttttta tgtataaata gcttctaata gttcgggtat tttgttttta taaccatttt
     4681 tattcttatt tttaaaaaaa tatttcgaaa tatactagac aattcagaaa attttgttta
     4741 atattattcc aataaaattc aaatctttat caaatgaaat tccaataaaa acggaaaaaa
     4801 agtgaaaaga ggaatgaaaa aatgacagaa ttgataattg gcggagtcac agcaagcaca
     4861 ttggcagaaa agtataagac acctctgtat gtgtatgatg aagaaaagat gagacaaacg
     4921 atgctgtcat tcaagcgagg ctttgtatcg aaacgttttg atacaaaagt gctttacgcc
     4981 tctaaagcat tccaaacgat tgagatgctg catttagttc gtgaatatgg attcgggcta
     5041 gatgttgtca gtggtgggga aatctatacc gctttgcaag ctgattttcc tagagatact
     5101 atctacttcc atggtaacaa caagacgcca gaggaactga tctatgcact tgaaaatgat
     5161 ttgttgcata ttgttgccga taatttgatg gaagttgaat tattagcaga attaagccaa
     5221 cgctatttaa aaaaaatctg tatcatgctt cggttgaatg tcggagtaga agcacatact
     5281 catgaatata tcgtcactgc gcatattgat tcaaaattcg gcatgagcta tgaaagtaaa
     5341 gagtgccaac aagcccttca gttgataaaa gaaagcagct atctagagtt agaaggattt
     5401 catgctcata tcgggtcaca aatttttgat gttactgctt ggttagctga aatcgacaaa
     5461 ttgacgaatt acttaaccga ttttgaccaa cctctttcct tgaatcttgg cggagggttt
     5521 gggattcgtt atacacaaaa tgatcagccg atcccagtcg aagaggcact aaaaaaactg
     5581 attgaatata cggaagaagc tttggaaaaa cgaaaactga ccattcgtca attactgatc
     5641 gaaccgggta gaagtattgt tggagaagct ggtacaacac tatacacgat tggtttcatc
     5701 aaaaagacac cacataaaaa atattacttt gtagatggcg ggatgacaga taatatccgt
     5761 ccagctctgt atcaggcaga atacgattgt gatttagcag caaaactgtc ggaagaaaaa
     5821 acagagaaag tgacgattgc aggaaaaatg tgtgaatcag gcgatgtggt aattaaagaa
     5881 gcttatttgc cgaaagcaga atcgggtgat cttttagctg tttattccac gggagcttat
     5941 ggatactcga tgagcagcaa ctacaatcga gcgacacgac cagcagttgt ttttgtcaat
     6001 aatggccaat caagactgat tgttcgcaga caagcatttt ctgatctact aaggggggaa
     6061 gtttcctatg atccttcata aatataatgg ctgcggaaat gattttatct tattagatta
     6121 cgcagaagac acagattata gtcaattagc cgcttcttta tgcacaaaag aaaaatatga
     6181 tatagacgga ttgatcgccg taaaaatcga tccgttggaa atgatctatt acaacaaaga
     6241 tggcagccgc gcgccgatgt gcggaaatgg cattcgctgt tttgctcgat acgtaaatga
     6301 tcagaacatg atgaatgaac aggaattcaa cgtagagaca ttagcaggca caagaaaaat
     6361 tacggtgttg gatccctcac ctttttattg cagcgtagat atgggagtgc cagatgattc
     6421 tccggaaaaa atcaaagcta atcaaactta tccaataaaa aaccaggaaa tagagatcaa
     6481 tggtgaaaaa gtaacgatca ctagtttatt tttaggaacg attcataccg ttgtttttgt
     6541 tgaaaatgcg cgtgaggaag tctggagaaa aagaggtgaa ctgatctgtc atcatccttt
     6601 gttcaaagaa aaaaccaatg tcaactttgt agaagtagtc aatgaacaag aattgatcgt
     6661 ccgtacctat gaaagaggag taggttggac acttgcttgc ggtacaggtt gctgtgcctc
     6721 ttatgtggtt gctaaggaaa ctgggaaaat ttcagcagat caggtagttg ttcacttgga
     6781 acaaggagac ttgatgatct ctggtgagga tgcgatacaa atggctggcc cagcagttca
     6841 tgaatggaca agaatattgg aggaatgaag atgataaaag gttcgatcgt ggcgctgatc
     6901 acgccgatga atgaagaggg atcagtagat tatgctggtt tggaaaaatt gatccaattc
     6961 catttagatg aacaaacaga tggattatta gttttaggta caacaggaga atcttctacg
     7021 ctcacacaat ctgaggagga gcagatcctt caactgacag taaagaaagt ggctggccgt
     7081 gtgccggtga ttgcaggagc tggaacgaat aatacgaaag aaacgattga aaaagcgaag
     7141 cattttgctt ctttgggggc agacgcactt ttggtcatca ccccttacta caacaaaaca
     7201 agtgatgcag gactagctgc tcattttaca gcaattgcag aagcctcacc gattccgctt
     7261 attttgtata atgttccttc tagaacaggc atgtcgattc caatccatgt attagtaaat
     7321 ttagcagaac atcctaatat cattggtttg aaagaagcct caggagatat ggcttatgtg
     7381 atggatgccg ctcggttgat tggagaagaa ttcttccttt acagtggaaa tgatgatttg
     7441 atcctaccag taatgagtgt aggaggaagc ggtgtaataa gtgtatgggc gaatatccaa
     7501 cctaaaatag tccatgaatt agtaaaagat acgcaggacg gaagatggca gcaagcaaaa
     7561 gaaaagcaat taaatgcact tgaactgatc catgccttgt tcagtgagac caatcctatt
     7621 cctgtcaaag cagccatgtc attattagac ttaccttccg gacctctacg cctgccttta
     7681 gtttctcttt caaaagagaa aaagaaacaa ttagctcagc ttttgttaaa agaaaggacg
     7741 gttgaaaaat gaaaatcgga ctgattggca gtggacagat gggaaataga gtcgaagaag
     7801 ttgcacaaga aagaggagat acggtccttc ttttacggac ggcacctaaa gaagcaacta
     7861 cgcttgcttt tacggaactg ccagaactgc ttattgattt ttctcatcca gataatttag
     7921 agaaaatcct ttcgtatagc ttatcatatc aacttccgtt agtcattggc acgacaggtt
     7981 atacacaatc acagttccaa gaaataaaag aacatgccaa acatgtacct gtgatgtata
     8041 gtgctaattt ttcgttcggc atcatggtga tgaatcaact aatcaagcaa gcagctgcaa
     8101 tgttgcaagg ctggcagatc gaactgattg aaaaacatca tagtcgtaaa aaagatgcac
     8161 catctggtac tgcaaatatg ctgcttcaaa caatccaaga agtgcaaaag ctgcagcccg
     8221 tatacaactg gcaagggaaa aaaagagaag aaaaccaaat tggtgtacac agtattcgtg
     8281 caggttcttt acctggtgaa catgatgtat tgtttgctgc tactgatgaa atatttacga
     8341 tcaaacatga atctttttct aatcgaatct ttgctgcagg cgcagtagaa gcagcggact
     8401 ggttgaaaga tcagtcacca ggttattaca aattagaaga tttattaatg gacaaagggg
     8461 tgtaaaaaac gtggaagctc aagaaatcat ccgctacatt caaacggcat ctaaaaagac
     8521 gcctgtgaaa gtctatctga atgtaaccga gccaatcgaa tttaaagaaa gtaaagtttt
     8581 tggtgaagga agcagcttcg ttgtttttgg tgactatgag acgattgctc cggttttaga
     8641 agctgaatca gaaaaaatca ttgaaatcga aatcgaaact gctgcaagat acagcgcagt
     8701 tcctttgcta gatattaaaa aaattaatgc tcgtatcgaa ccaggtgcta ttatcagaga
     8761 tcaagtgtca atcggcaaca atgccgtgat catgatggga gcgattatca atattggtgc
     8821 agtaatcggt gaaaacacga tgattgatat gggtgctgtc ttaggtggac gagcgacagt
     8881 gggcaaaaac tgccatattg gtgcaggagc tgtcttagca ggtgtgatcg aaccagcatc
     8941 ggccaaaccg gttatcgtag aagacggcgt actggtaggt gcgaatgcag tgattgttga
     9001 aggtgtccat atcggaaaag atgcagtcgt ggcagcagga gcagtggttt tagaagatgt
     9061 ggcagcagaa actgttgtcg gtggaatacc tgcacgtgta ttaaaaataa gtgatgatca
     9121 gacaaaagaa aatacagcat tgattgctgc gttacgcgaa ttatagagga gatggagaaa
     9181 aatgcgcgaa atagatacct gtattgtggg ggcaaccgga ttagtcggcc aaacgatgct
     9241 caaagtgtta gaagaatatg attttccagt aggacgattg aaattattag cttcaaaacg
     9301 ttcagccggt aaagtttgtg tattcaagaa aaagaaacac actgtacagg aattgacaga
     9361 aacttcattt gaaggatatg atttagcgct attttctgca ggtgcttctg tatccaaaga
     9421 gtttgcgcca atcgcacgaa gtcagggact atttgtcata gataattcct cagcatggcg
     9481 ggaagattcg gatattccat taatcgttcc agaggtcaat ctggaagact atacattgaa
     9541 tcggctgatt gcaaatccaa attgttcaac cattcaagcg gtacttccgc ttaaagcttt
     9601 acaagaagcc tttggtatcc agcgagtcaa ttattcgacc tatcaagcag tttctggttc
     9661 cgggcaaaaa ggcattgaag atcttcatcg tacgcaaaat gacgaggaac cagttttgta
     9721 tccttataat attagccaaa cggtgattcc ggaaatcgat cagcctttgg aaaatgggta
     9781 tacaaaagaa gaacagaaaa tgataaatga gacccgtaag attctgcatc agcctgcttt
     9841 gccagtatca gcaacctgcg tacgtgtgcc aatcgaaaat ggacatggag tctctgtggc
     9901 agttcaactg caaaaagagt ttacgacgga agaaattaga gagtgtctca gccagtttcc
     9961 agggattcaa ttagaagatg atctagcagc acataaatat ccgacgagca ttcttgctag
    10021 aggaacagat atggtttatg ttgggaggat cagaaaagac acttctttag agaacggatt
    10081 gttgttctat acgactgccg ataacatacg caaaggagcc gctgcgaatg ccgtacagat
    10141 tgctgcagct ttaatgcaga aaatagcgga ggtggcaaga tgagtaatac gctgaaagta
    10201 gcgaaattcg gcggaacatc gatgtctcat gaagagcaat tcaaaaaagt ccgtacaatt
    10261 gttctttcag atcccaatcg ccaagtgatc gttgtttctg ctttaggcaa acgtacgtca
    10321 gatgatgaca aagtaacaga cttactttat ttgatttatg cttaccttca gcatcatgtt
    10381 gattggacac ccctttggaa aaggatttgt gatcgtttca ttcaagtaag agatgttctt
    10441 gccctgcagt tacctattga agatgaacta gcacgcatac agaaacaact ggaagacgaa
    10501 aagataactg aagattattt agtaagtaga ggtgaatatc tgactgctca actgatggct
    10561 gagtatcttg gttaccagtt tattgacgca aaagacctga ttttctttga ctatgatggg
    10621 aaaatcgatc aggaaaaaac agcacaggaa atcacaaaag catttaaaag atatccgaaa
    10681 atggttgttc cagggtttta cggggcgaac cctgcaggaa aagaaaaatt attgggccgt
    10741 gggggaagcg acattaccgg cgcgatactg gcgaagatgt tgcaagcgga aagttatgag
    10801 aattggacgg atgtttcagg aatcatgatg gctgatccgc gaatcatcga tcatcctaaa
    10861 atcatcgagg aaatctcttt tagagaactg agagagatgg cttatatggg ggctaacgtt
    10921 cttcatgaag cagctgtttt cccagttcaa gaggctgata tccccatcca aataaaaaat
    10981 accaatgatc ctttcgctca agggacaaaa atatcgaatc acgaaataga aaaggaaaat
    11041 ggactgacag gaatagctgg acgcaaggat tttctttcca tcactttatt caaacgtcat
    11101 atgtccgatg aaatcgggtt tatctggaaa gcaatgagca tttttgctaa acatgagata
    11161 tcaatcgaac atattccttc agggatcgac aatatcggag tgatcgtctc agcagaagcg
    11221 attgccgata agttatttct catcacgaaa gaattaaaag aaacacttgg cgtggaagaa
    11281 atcgtcgtga tcgaagactt agctttgatc tctgtcgttg ggggtccgca taaagaattg
    11341 attggattat ctgggaaagt gttatcgata ttgaacaaat tagaaattcg cacatcgatc
    11401 ttatcacaag gagcacagga gttgaatctg atcatcggtg taccgaataa tcaatatgaa
    11461 acagtagtga agggtatcta tgaagggatg gtgaatacgg atgctgaaag aatatcgaat
    11521 ggcactccat caaattcctg aaatcgggtt tcatgaattc aagacaaaaa aatatttata
    11581 tgaacaagta aaaaacagcg aggccgttat ccatgaaatc ggcgaaacag gactactcct
    11641 ttattttgat aatcagcaag aagaaacgat tgcttttcga acagatatcg atgcactgcc
    11701 agtcaaagaa gaaacgaatc tctcttttgc ttctaagcat gaaggttata tgcatgcatg
    11761 tggtcatgac ggacatatgg cgatgctttt agggttgact gactttataa ctaaacacat
    11821 tgaggatctt atttataatg tagtactgat ctatcagcca tcagaagaaa tttccggtgg
    11881 cgcacaaagc gttattcagt caggattgtt ggataaatat gaagtacaag caatatttgg
    11941 ctttcatcta tggcctggac tagctaaggg agaagttttc tctcgaccag gacctttgat
    12001 ggcacaaagc agtgaaacag acattatcat taaaggccag tctgcacata ttgcttctag
    12061 tgaaaaagga atcgatagtc tggaggcagc tgtacgattt ttgaagaatg tttatgattt
    12121 tgaccggcaa ttaccggcag agtggaatca tctaatgaaa tttggccaaa tccaaggtgg
    12181 aacgatccga aatgttttag ctggagaagt tattgtatcc ggaagtatcc gttcttacag
    12241 ttcagatatg caaaattatt tgaagagggg attagaccat ttagcagaac agtttcactc
    12301 tacagctact atttcttttc gctataatga cggctatcca gctgtaatca accatagagg
    12361 cttatatgca gcactgttag attcaaacaa actacatgaa ttagatgctc cagttcttca
    12421 agccgaagat tttggcatat atacgcaaca atatccatgt gtatttttct ttttaggtgt
    12481 cggtgatacc cctgctttac atgatccgac gttcgatttt gatatggatg ttttagaaaa
    12541 tggtgtagaa tggtacaaaa cgattctttt tacagaaaat cttcttgtaa aaatcgatag
    12601 cagttcaaaa gaggagcgag taaataatga tgaatttatc caatcgattt agtcctaggc
    12661 tttctagaat agaagtatca aaaatacgac aattcgatca gcaaatatcg tcgataccag
    12721 atgtgatcaa actcaccctg ggagaaccgg attttccaac tcctgaacat gtcaagcaag
    12781 caggaatagc agcgattgaa gaagactttt ctcactatac cggaatgaga ggattagagg
    12841 aactgcggga agcggcttgt atatttcagc aacagcgtta tggattaact tatgatccgc
    12901 aaacggaagt tttgacgaca gttggtgcaa cggaagcgat tgcctctgcg ttgttatctg
    12961 ttttggaaga aggagacaag gttcttattc cagcgcctgc ttattcagga tatcagccat
    13021 tagtggaact agctggtgct gaattgatcc cgatagatac ttctgacaca gggtttgtct
    13081 gtcagccgga acaatttgaa cgagcatttg aacaatatgg atcagctgtc aaagcagtca
    13141 ttttaaacta tcctaacaat cctactggga cgacattgtc tgcaaaacaa ttgaaagcaa
    13201 tcgctgaggt tttaaaaaaa tatccggtat ttgttataag tgatgaagta tatgctgaat
    13261 tgacttatag tggaacgcat atgtccatag ctacttatct tccagaacaa acaatcgtga
    13321 taagcgggtt atccaagtca catgcgatga ctggttggcg agtgggcttt atttttgctc
    13381 aaaaagccct tattgacgaa ctaattaaag tccatcaata tctggtgact gccgctacaa
    13441 cgatgtcgca aaaagctgcg gctgaagcgc taataaatgg ggtgaaagat agcgagaata
    13501 tgaaagagca atatcaaaca cgaagagact atctggtcca gcaattggcg cctttaggat
    13561 ttgaagtgac ccagccaaac ggtgcatttt acttgttttg caaactccca gctgccattc
    13621 aaactgactc ttggcagttt tgtgtagaat tagcggaaca ggcaaaagtt gcgtgtatcc
    13681 caggaattgc atttggccca gaaggagaag gttatatccg gatttcttat gctagcagca
    13741 tggaaaaatt acatgaggcc tgcaaacgga tcaaagactt tttaacagaa cagtgattca
    13801 tagattttat caaaaaaaca atcgttaatt ttgcggtata ttctgcaagg agacgattgt
    13861 tttttaaatt tagttcaaat gaacgcgttt cttttgattc ataactaatg taatgttaag
    13921 tttaaattgt ttagaatgat tttaaaagaa tattcaagga ggtttcatca atggaaaaaa
    13981 tcaaagttgt cattgtaggt gcatctcatg gagggcatca atcgatcttg gaactattga
    14041 cacgatacgg tgaaaatgta gatatcacgc tatttgaagc aggagactat atttccttca
    14101 tgtcatgtgg tatggagctg tatttagaag acaaggtaac agatgtaaat gatgtacgta
    14161 atttcagacc agaaaacttt ccgcaatcca atgttcatat tttaaataat cacgaagtaa
    14221 aaacgatcga tgccgataaa aaaacagtaa cggttactag cgcatcagat aacgaaacaa
    14281 aagaatatcc ttatgacaaa ttgattttga gttctggtgt caaaccaaac aatctgccag
    14341 taccaggagc agatctagaa aatgtctact tgatgcgtgg atacaattgg gcaaccaaaa
    14401 tcaaagaaaa attaaatgat ccggctatta aaaaaatcgc tgtcgtaggt gcgggttaca
    14461 ttgggatcga agcagcagaa gcaagccgaa aagctgggaa agaagtcgta ttgcttgatg
    14521 tgatcgatag acctttaggc acttatttag atgctgagat gacagatatt ttagaacaac
    14581 acctaaatga aaacggtatt gaagttttga caaatgcaaa aatcgaagct tttactggta
    14641 acggaaaagt tgaggcaatc aaaacaagtg aaaaagaaat cccagcagat ctagtcattc
    14701 aagctgccgg tgtgaaggca aatacagaat ggttaaaagg gatcgtcgac ttagatgaac
    14761 gtggctggat ccaaacaaat gaatacttgc aaacgaatct tccagatgtg tatgcagtag
    14821 gagatgctac acttgcttat tctatcccag cacgcaaaaa aatgccgatc gctttagcga
    14881 ctgttgcacg tagagaagca cgttatgtag taaaacatct ttttgaagaa gtaccaaaca
    14941 aaccgttcgg cggagtcgta ggttcctctg cattaagtgt ctttgactat cattttgcag
    15001 caagcggatt gaatagcttt acagcagcaa aagcaggagt agatgtacga gtgtcttatt
    15061 atgaagatac gttacgacca aaatatgtac cagaagaaca aggaaatcca aaagtgtcca
    15121 tccaattatt ctcggatcca atgacccatc aattgctagg aggagcagta ttatctacct
    15181 atgatgttac aggtcaagga aatgtattgg cactagcgat ccaaaacaaa ctgactttag
    15241 aagaactagc agaagctgac ttcttcttcc agccaggctt tgaccgacaa tggagcatgt
    15301 tgaatctagc cgctcaacaa gcgttaggag aagaaccatt tgtagaataa gaaaacgaaa
    15361 tatttgaaaa gaggttgtga aaaagctgcc ttgcatcaag taataagaac agccaagtaa
    15421 aaacagcttc ttatgttttt aacttggctg gacttattac cgcagatgca agcttttgaa
    15481 caccgtttat tttcaaaaaa gggactgtga cacgatttgt gtaccgaccc ccaaaagtta
    15541 gaccaaaaaa tctaactttt ggaggtcggt attttttatg gttaaatata cttttgaact
    15601 taaattaaag atcgttcatg attatttgga tggcaaag
//
LOCUS       DAMEET010000065        14979 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.066, whole genome shotgun sequence.
ACCESSION   DAMEET010000065 DAMEET010000000
VERSION     DAMEET010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.066"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            331..1092
                     /locus_tag="PB780_001881"
     CDS             331..1092
                     /locus_tag="PB780_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDE0733933.1"
                     /translation="MHTPEEIMELSIHLGTKKIHKPILAKLILGFIGGAMISLGYLAY
                     VRVSASIPESMASLTSLVGASVFPIGLLVILLGGGELITGNMMAVAIAFMDKKVTFSQ
                     LVKNWSVITFANIIGAIFVAYFFGHFVGLTHSGVYLDQVIHIADHKIAVSWWQALGSG
                     IGCNWFVGLALWLSYGAKDAAGKVLTIWFPVMIFVAIGFQHSVANCFVIPAAIFEGQA
                     TWLEFLSNFIPVYIGNIIGGAIFVSGFYYVSYKHH"
     gene            1320..1514
                     /locus_tag="PB780_001882"
     CDS             1320..1514
                     /locus_tag="PB780_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733934.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            1783..2082
                     /locus_tag="PB780_001883"
     CDS             1783..2082
                     /locus_tag="PB780_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733935.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(2179..2613)
                     /locus_tag="PB780_001884"
     CDS             complement(2179..2613)
                     /locus_tag="PB780_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDE0733936.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(2845..3555)
                     /locus_tag="PB780_001885"
     CDS             complement(2845..3555)
                     /locus_tag="PB780_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDE0733937.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(3545..4405)
                     /locus_tag="PB780_001886"
     CDS             complement(3545..4405)
                     /locus_tag="PB780_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDE0733938.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(4410..5048)
                     /locus_tag="PB780_001887"
     CDS             complement(4410..5048)
                     /locus_tag="PB780_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDE0733939.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(5063..5362)
                     /locus_tag="PB780_001888"
     CDS             complement(5063..5362)
                     /locus_tag="PB780_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDE0733940.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKEVTVIGIKNVMSAKEISEKFAETKWYQKNK"
     gene            complement(5405..6889)
                     /locus_tag="PB780_001889"
     CDS             complement(5405..6889)
                     /locus_tag="PB780_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDE0733941.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPEGYFLIVEDYDTYAEKQRV"
     gene            complement(6909..7370)
                     /locus_tag="PB780_001890"
     CDS             complement(6909..7370)
                     /locus_tag="PB780_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDE0733942.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGEDEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(7388..8455)
                     /gene="ulaG"
                     /locus_tag="PB780_001891"
     CDS             complement(7388..8455)
                     /gene="ulaG"
                     /locus_tag="PB780_001891"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDE0733943.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADSKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            8673..9443
                     /locus_tag="PB780_001892"
     CDS             8673..9443
                     /locus_tag="PB780_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDE0733944.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(9716..10544)
                     /locus_tag="PB780_001893"
                     /pseudo
     CDS             complement(9716..10544)
                     /locus_tag="PB780_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            11111..12346
                     /locus_tag="PB780_001894"
     CDS             11111..12346
                     /locus_tag="PB780_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDE0733945.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(13345..13605)
                     /locus_tag="PB780_001895"
     CDS             complement(13345..13605)
                     /locus_tag="PB780_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDE0733946.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(13632..14933)
                     /locus_tag="PB780_001896"
     CDS             complement(13632..14933)
                     /locus_tag="PB780_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDE0733947.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEKAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNATLLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPMIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4772 a         3005 c         2389 g         4813 t
ORIGIN      
        1 gattttgaca ttttttctct cctataataa ttttttcagt tattcatttc cttttttata
       61 aaaaatgtag attgttttga agtaataata tgctgtagtt tcaagaaaat ttacaggaaa
      121 tttgataact gatattattt actttttgac caccatatac atgactttta tttttgtttc
      181 ttttttagaa tcaaatgtga ttaaatagaa aattttacaa taatatgcac atataaataa
      241 taagatgata taatcaacaa agaaaatgtg atttttttca ctatatttta tcacatgaaa
      301 attcattaag aaagtaggaa tcgaagtatt atgcacacac cagaagaaat tatggaactc
      361 agtattcatc ttggaacaaa aaaaatacat aaacctatct tagctaaact gattttaggt
      421 tttatcggag gagccatgat ttctctaggt tatttggcat acgtacgagt ctctgcttct
      481 atcccagaat ctatggctag cttgactagt ttagttggtg cctcggtttt tcctatcggc
      541 ctgctggtca tccttcttgg cggtggtgaa ttgatcaccg gaaatatgat ggctgtggcg
      601 attgccttta tggataaaaa agtcacattc tcacagctgg ttaagaattg gtcagtgatt
      661 acttttgcaa atattattgg tgcaattttc gttgcctatt tctttggaca ctttgttggt
      721 ttaactcatt caggtgtcta tctcgaccaa gtgattcata ttgctgatca taaaatcgct
      781 gtttcttggt ggcaagcatt aggctccgga ataggttgta attggtttgt cggtttagcg
      841 ttatggttat catatggagc aaaagatgcg gccggcaaag tactaacgat ttggtttcca
      901 gtcatgattt ttgtggcaat cggtttccag catagtgtgg ccaactgttt tgtgatacct
      961 gctgccatat ttgaaggtca agcgacttgg ttagaatttc tctcgaattt tatccccgtt
     1021 tatattggaa atatcatcgg cggagctatt tttgtttcag gtttttatta cgttagttat
     1081 aagcatcatt aagattgaag gtaatgttca ttcgagcatt accttcaatc tctttatata
     1141 ctatttatat tttaagaaca atagcctttt attatatata ttattcaaat tagttatatc
     1201 gagtgaaaaa aactttcaaa ttgattcttt ttatttatca taaagatata gatacataat
     1261 cattttcact tacatatatt tgaatattaa ctaaaaacag caagaaagaa gaggatcaaa
     1321 tgtattatgt agaagtaaaa acaaaaggcg tcaaaaacaa acaatatgtg aaaggtatca
     1381 gtaacgaata tcctttactt ggttcatgga aagaagctgc tcctttttcc aaaccatgtg
     1441 ccataaaaat aaagaacgag ctagaaaaag aactgacttg tggcaaagct gttgtagata
     1501 tcatcgaaaa gtaaatccaa tgtatagaaa ggaagatcac acatgaaaag cgacgtacaa
     1561 attgctaaga ttatagaatt agaagaagaa gacgcagtgt tgacaattga aaaaaaataa
     1621 atgtttgtat accatatgat tatttcaata tgtatcctgg aattggagat ctcgtagagc
     1681 tatatcaaga tgaatcaaat atacttgtca ttcatcatga aatatagaga cttataaaat
     1741 tgtttatttt tataatgtat cagtatagaa aggaaataaa acatggatta tactgttgaa
     1801 tttgacagtg aactacttaa gcaatttcca caagaagaac atttcatgta taaagaagca
     1861 atttcccaca aagtgacaga gtatttggag cataattttt ttcgtataaa gccagtgcgt
     1921 actcgttcgg caaaaggaat atacgagatg aaagtcaaaa ttaataaaaa aaattatcgc
     1981 atagcatttg ctttaaaaaa aaatcatgtg ttcatttttt atattagttc gaatttgcaa
     2041 aaaattgtat ttgataaaga ggtgagtaaa aaaataaaat agctgagcat tggaggatag
     2101 attgagtgat actaagtcta tcctcttgtt tttctaaaga actaagctag aattttttct
     2161 tttgtacgtt attatatgtt atttgtcatt accaaaaagc accttttttc ttctagtatc
     2221 tatgacataa gaaataccac ccatttcctt tacttgaaca aatcctactg attcataaaa
     2281 tttttttgtt ttctcagtat catctgtcaa taattcgatt tggactggtc cataactagt
     2341 gatcttctcc aaagcttcat tcaaaagcac tttgcctatt ccttggcgct gatacctcgg
     2401 atgaatgagg atatcttgaa taaatgcgat cgtcgcaaaa tcagtgatcc aacgaacgat
     2461 tccgataatc ttcccttccc cattacgctt tatcagagat tcagattgtt caaatgctct
     2521 agctaacctt gcagtgtctt ttgtgtaatt cgtccaacca acgctcttat ataattcaga
     2581 aatctcatcg atcataaggg aatcaatatt catttttttc ttcctttctt atacaattct
     2641 ccctctaata atacagttct ttgactattt aatctatcac atataactct cctgtaaaga
     2701 ttctctcgat tccttaaaat ctctctttcc ttttagaatg catccttttt gataaataaa
     2761 aaggttgtga taaattttct tcacaacctc tgtttctctc tatcaagtgt tatcttttaa
     2821 ttttctaccc ttaccctagc atctctattg cccgtagtat gcatactttc catgtttgcg
     2881 aaaataatgt ttatctagta aatattgcgg gatttcacat atattggggt tgatcagctc
     2941 gttttctttg gccatcagac aaatttcatc taagatcaaa ctgttttcta ctgctttcat
     3001 aggtgaatcc ccccatgtga acggtccatg tccatagaca agtactccag ggacttcgtt
     3061 tggatctaat tttctttcct taaacgtctc gacaatcaca ttccctgtat gaacttcgta
     3121 agcttctctt acttcttctt tcgttaattg gcgtgtacaa ggaaccttac cataaaaagc
     3181 atcagcatga gtcgtgccat aagctggcaa atcgcgtcct gcttgcgccc acatgacgga
     3241 gtgcgtggaa tgagtatgag taatagcagt tatatcttca aaagtttgat acaaaacaac
     3301 atgtgtgggg agatctgaag atggttttaa gctatcttct tctattatat ttcccgacaa
     3361 atctgttacc accatttgat ctgcttgcat gcattcatat ctgacaccgc ttggcttaat
     3421 gacgatgact cctagctcac ggtccacttc actcacattg ccccaagtca atttgactaa
     3481 tccgtatcgc ggcaatgcca gatttgcttc aaggacacgt tgcttcattg ctgcaatttt
     3541 ttcttcacaa gacatcatag ccagcctcct tcaatttaga acttaagtag tcttttgcct
     3601 gctggatttc tcttcgaaag tcttggcttt tttcactcca catttcaatc agaaatgttc
     3661 catcataatc tagacgtttc aaatgtctta ataatccaag aaaatcaaca caaccgctac
     3721 caaaaggaac atcacgaaac tggcctgaat attcttttgt tacagggtaa gtatctttta
     3781 agtggatagc agtgatataa tcgatccctt tctcaagttc tcgagccgga ttattttccg
     3841 gccatgcgga caaattgcct aggtctggat atacttgtag aaaaggggaa tgaatctgtt
     3901 ctttgatttc taaaaatttt gaaatggagt tcataaatgg atcatccatg atttccacgg
     3961 aaagaatgat gccatattta gaagcttcct ttacgctttc ttttattcct tcgataaagt
     4021 cttctctgga attcattgat ttctcttcat aataaacatc gtatccagct atttggatta
     4081 ctttgacgct tagattatgc gccaaatgga ttgctttttt cattagttgt ttcgctgctt
     4141 ttttcttttc tggatcagca gaaccaaacg gaaatcgccg atgcgcactt aaacaaatcg
     4201 aatggatacg tacaccggag ctgaacatct cttctctcag ctcagctatc ttttcttcag
     4261 accaatctaa tcgtgctaac cgctcatccg tctcatcaat cgacatctcg ataaaatcga
     4321 agtccatttc ttttacaagg gaaaaacgtt ctttccatga aatatctttg ggtaatgctt
     4381 tttcataaat cccaatttga gccatttcct tacccccaga tacgaatgat ctcatctttg
     4441 aagttttgtg cggccttcat cggatcattt gctgctgtaa taccacgacc agtgataaag
     4501 gtatacacat cgatcccttc aaataagtgt aatgtgtgtg gatttaatcc accagttaca
     4561 gaaacattaa agcctagttc aatcagtttt ttgatcttcg ataaatcttt ttcaccccaa
     4621 gtttcaccag ataacagcgc atcacggctt tgatggtaga ttacctgccg gataccgatg
     4681 ttatgccaat ccatggcttg ttcaaaagtc caattgccgt agagttctac ttgcaattca
     4741 cctatttctt tttgtgctgc ctccatagtt ggaatcgttg cacaacaaat gactgtcatc
     4801 cagtctgctc ctgcttttgc aacattactc gccactgttc ctccagcgtc tgcacatttc
     4861 gtatcagcga ctagtttttt atctgggaac atactgcgaa tacagcgaat cgctttttgt
     4921 ccttcctgta agcaaagaat tgtgcccact tcaataatat ctactacatc accaactttc
     4981 ataacatctc ctaatgcatg ttctaagtca ttgtgatcta acgcaatttg caaattcggc
     5041 ctgctcatct tagttccttc ctttacttgt ttttttggta ccactttgtt tcagcaaatt
     5101 tttcactgat ttctttggct gacatcacat ttttgatacc gataactgta acttcttttt
     5161 tctctgcctg agtaaacata ttcaaaaaat tgactggcgt aaaaactaca tcgtattggc
     5221 cagctgaact ttttccctct gaaatggcac aatgatggat tttggtaatt ggtacgttta
     5281 gttctttcat tgctttttcc acacttctca tcatcatcaa gcttgttcct gatccatttg
     5341 cacacgatac taaaattctc atcttaatcc ctccattatg agtgttgtct aatcagattt
     5401 tcttttatac tctctgcttt tctgcatatg tgtcatagtc ttctacaatc aaaaaataac
     5461 cctcaggatt tttccgatat tgtaactgcg gaatagccaa taaaccaatg acaactacgg
     5521 caattccagc ataacctagg actttcatga ttactgtaaa caatggccaa gctgttgccc
     5581 agtcgaacat cccgatataa ccaccatatg ttgacatccc aatccatgta gcgaataatg
     5641 ctgaaccgcc tacttgtatg attcctgaaa gaaacgggaa caggcaagcg gctttgaatc
     5701 cacctcggtt attggcataa actgctataa cggcattatc aaagaacaac ggaatgaaac
     5761 cggctatgac gatcgttggt gatttcaata aaatcagtaa accaatcatc aaaaactgtc
     5821 ccaaagcacc aaacaagaac ccaatcgtta ccgcatttgg cgaaccaaag ccaaaagtag
     5881 cagctacgtc aattcctgga acagctcctg gtaaaattgt gtttgagatt ccttggaaag
     5941 attgagttag ttcatcgacg aatgtccgaa cacctaattg caagatcgcc agatataccg
     6001 caaaatttaa agaagtcgtc ataatgtaga ataagaaact ctgtccttct tgcatgaatt
     6061 gcgcttgaat caaataatct ttgcctagta caatcagaat gatcccaaag aaaaatagca
     6121 tgagtaacga agtagcaacc atattctcat tgaaaatcga caagaatcct ggtaatttga
     6181 tatcttcgat ttttttgtct tgcttgattg atctattttt cttttcatcc cgctttttca
     6241 tccactcagc taattttgca aatataaaga ttccgaacat ttgctgatgt gccactgcaa
     6301 atccggcacc ttctgtcaat tcctgcgtga ttcctactgt taagttagaa gctactgccc
     6361 aataacaacc taaaatcagt cccataaaaa tcaatacttg tatcctacct agatcaggaa
     6421 agcaaaacag taaaatccaa aaagcggtag ctgcttgttg aacctgaacg tttccggtag
     6481 tgaaaacgga acgcagtttc gtatatttct gaaaacggac taataagata tttaaaacaa
     6541 atgcaatcag taataaaatc atcacatctc caaaggtacg gccaaatgtc tccattaatc
     6601 cagcatccac agcattttgt ccaaaataag gatctgtaac catggcatcc aaattgaacc
     6661 gttcctttag tccaaccaaa atcggccgaa aattattgac caaaccacca gagccaacag
     6721 tcaaaataaa gtaaccgacc gttgccttta agaatccagc gatgcattca tgaagcgggc
     6781 gtttcagcaa aatatagcct aataacacga taaaaccaat caggtatgct ggctgcgtca
     6841 agatatttgt tgcaaaatac gtccaaatgc ttaacaaaaa ttcccccatc ttttttctcc
     6901 tctatcaatt aattatattc tgccatcaca acttggtaat catcgatgga gttcgtagag
     6961 atgagacgct caaccaatcc atctgtcatt aataattcgg ataatttttg gatattttct
     7021 agatgttctt ctggatcttt tgctgcaagt gtaaaaaaca aactagccgt tttctcctca
     7081 tcctcccctt cactttcaaa acggattggc tgtttcaact tggtaaatga aatagctgtt
     7141 ccaaaaacac catcactttt ttctgaagaa tgcggcattg ctacttgagg tgcgatcaca
     7201 atataaggac cgtatttttc tacacaatgg atgatctcct ccacatacgt ttcatcaata
     7261 atttccttat cgatcaatac aagacagctt tcggcgatag cttcacgcca atccgtgatt
     7321 tctttatctg ggtaatgaat caattgatgg tcaaaaaagt atttcagcaa gaaactcccc
     7381 ttctttctta taaaaacgac ggaaacggtg tatcattttc aatcgagaag acatcttcaa
     7441 atcctcgata aaaattaaac tcaagatcat ctttgtttgt cggatagatg aatttcccgc
     7501 ctacttgcca gatataaggt ttaaatccat acttcaggcg atcctttttg aatttccaaa
     7561 tttcagtgat ctcttttgaa tcagccataa aattagacca aatatcgtag tggacaggaa
     7621 tcactacttt tgcattcaat gactccgcca tgcgcagcat atcaacagaa gtaactttgt
     7681 ctgttatccc tcgtgggttt tcaccatagg cccctaaaca gacatcgatt ttgtgttcat
     7741 taccatgttt ggcaaataaa ttggagtaat gtgaatctcc tgcatgataa agattccctc
     7801 ctgatgtttc gaataaatag tttactgcaa tttgatccat ctcctgaggc atttttcctt
     7861 ttaatacctc ttctggatct tcacaagtga ccaacgctgt ccgatcaaac gcttctaaag
     7921 ctactacatt gatgtctttg attttgactg catcacctgg ctttactgta atcgtttttt
     7981 cttcaggaat tccccatttt tgccacgtgc gaaccacttc ttttggccca atgaatgttg
     8041 cttcaggaca attttgatga actgctgcgg ctgtatggat atctagatga tcggaatgga
     8101 tatgtgtaac gattaaagcg tctacatttt ttatcgcaaa aggatcgatt acgaaaggct
     8161 gagtacgtaa attaggctgc atattttgaa cgccactcat acgcatcatc tgatggcctt
     8221 ttttcatcat gccattccca tgactacgtt ttcccgtccc acaccataga tcacataaaa
     8281 tattcgttcc ttcatgactt ttcagccaaa ttcccgtaca gcccagccac cacatagcaa
     8341 aagttccagg atccactact tctgtttcga tttcttcatt caagtatgtg ccccactcag
     8401 gaaatgtttc tactaaccac ttttcttttg ttacttcatt gacagtttta ctcatttgct
     8461 tgctcccccc atatcgttgt tccaacttac aagtaaatat taatgtgtat cagcatccat
     8521 ggaaaccctt ttatatgacc gtttgttttt tatatgattt tctttcagta atcaatgatt
     8581 gtttcagtat aataaagaca gatatgaatg tttaaatgaa caactagtca aataaatcca
     8641 tggatcaaga tgtccctcag gaggcaaaag tcatgaaaaa ctcacttgtc aatattgaaa
     8701 aacgtcaaaa agaaattttg gaactgcttc agcagactga acaactatca actttcgaat
     8761 tagctgaatc ccttgatgta tctattagta cgatacgaag agatttaaat ttattagaag
     8821 aaaaaaacga gatcatacgc aattacggtt attgttctta taattacaaa aaccagactg
     8881 attttgatca atctggtcct gaaaggatta aacaagcgat tgccagagaa gctagtaaat
     8941 acattaacga ttatgatacg ctgtttatca attctagctc gactgcgttg aaaacactgg
     9001 attttcttca atctaaccat ttgacagtca ttacaaacaa cgtgaaaatc aattactctc
     9061 cgcacaaaga taattgtagt tatattttaa ctggtggaga gatgcgcttt ccaaaagaag
     9121 cactagtggg cgacatcgca ttaaatacga tttcttcagt caatgcagac atttgtatca
     9181 ttggctgtag cggagtcgat gtagtacatg gggtcacaac aaagatatta aatgaatcaa
     9241 cgatcaacga aatgatggtc aacaagacaa tcaaatgtaa gattttagtc gctgatcatc
     9301 gaaaaatcgg actgacatca aagttcaaga ttgctgatat ttcagtcttt gattatttga
     9361 tcactgataa attttgttct gaaaaagtat tagaggagat cagaaatacg gggataaaaa
     9421 ccattcaagt agattcattt taagaaccag gacgtaaata tgtaaatagg attatcaaaa
     9481 aaatgaataa caaatttatc gataaatgaa aaacacgcta cgctcaaatt gataaaggaa
     9541 tcccaagatg tgtccatcca atcgatacca tcataatcta ttgcttcttt tataaccgtt
     9601 atatgttgcc taaataggaa tcaaacaata tcgaaaaaag gaggtatgac aaaagtcaca
     9661 cctccttttt tcgaataaac ggtattcaaa tcagaacact gatttcacaa ccactttatt
     9721 tcaggtcgtt ttcaatcacc tttaccgcgt aatcatttcc gttgaaacgc gctatttttt
     9781 ttgcgtccat atatctttct aaataatcat cttctcctaa ttgcttatgg caagaagcaa
     9841 tttcataaag caagtattcc aatgcataag tcgatttatg tttcttatta tactcaatac
     9901 ccttctcaca taattggatt gctttatcat aatcctcgat tttacggtaa taggtggcga
     9961 tatcataata tacaatgcag accatcagat tatcaaaatc agtctcactc aatattttaa
    10021 gtgtacgctc cacataaact ttcgcaaaat ctaattctcc ttttgacaag tagatcattg
    10081 ccacgtttgc agctgcttgt aagtgatgga tatctgtttc ggtcgtttgt tccaacactt
    10141 gattaaaata aaataatgct gcatctggtt tttttcgcat aaagatgatc aagcagtcct
    10201 tgataaaata aaatttgatt taaaagatca gaattactta ctttttcctg atcgatcgtt
    10261 tgtaaaattt tataagattc ttggtatttt gatacagaat tcaatgattg aatctcattt
    10321 atttttttcc tgattttgtc ttcttctgtt tctactaata tatcggagat ctcgacattc
    10381 agctttttgc aaagcgcact taagatattg atgcctggcg acatattctt gttttcgata
    10441 ttgctgacag ttgcttgttc acaaatgcct tctgctaatt gtacttgtgt taaccccttt
    10501 ttctttcggt attcttttag taatgctcca tctactttaa tcaaaacaat ttccttccct
    10561 ttaaaaaatt atttctcctt tatatcatac acaaaaaaaa gttctaaaca accaaaaatg
    10621 tttagaacag aataaaaatt ttaattttaa aataatattc aaattaaaca actggcgtat
    10681 ctgctccttg atttcttttt gtgttatctg aataattatc gtttacagtt ataacacata
    10741 gtgaaaattg tcatggcgag aaagtctaag aatattttta aacataacta tttctttctt
    10801 acgtgtgtat aatgacgaca gcacacctat caattaatat aattatttta ttttttatgt
    10861 tctcatgttt atttttaatg acacaacttc tcgtatttat ctggcacaac atataaatcg
    10921 atcaaaaaat tgttgcgtgt ttttttgaaa attggagttt caacttgatt acaaaattta
    10981 aatttagaag ttgtgaaaac agcgagagac agtttaatat tcaaaatata cataggctac
    11041 tcgaacgctg tgttttttag tatactagta agaaacgaag ggatagactc attccaataa
    11101 ggaggatttc atgaagacaa ttctttttgc tcctgcagta ttcaatttag ctgagacaac
    11161 acgaatgatt gaggttgcca aagctatgaa agaccgttat tactgtgtat tctttggctt
    11221 ttctaaaact tttgctcctt tgatcacaga aagtggcttt gagtatcatt tactagcgcc
    11281 gatccttacg aaaaaacaag aagaacaaat cataaaattt gaccagttgc gtacacttaa
    11341 aaaccctttt accaaagaaa tggttgcctc tcgtatcact agtgaacaga gtctgattgc
    11401 taagtttgag cctcgactga ttgtgacagg ttcgaatgtg acaatttttt tatcggcccg
    11461 caataaaaag attcctttag tatatgttaa accctatgct ttgagtgaac ctttttttca
    11521 atcagagcat gcgctgatgc cccacgagtt acgtttcttc ggccctttaa gcaatcccct
    11581 ttggcactgg acaaaaaagc gtatagtaaa tacccgatgg atccccaatg ccttcaaaca
    11641 cgctgcacaa aaagaaatgc ttcctttacc aaaaacaagt ttacaaatga tggatgctga
    11701 tctaaatctc atcacaacgc ctaaactatt tttggaagaa gaacagctgc cggaaaatta
    11761 tcgaatcgtt ggtccaattt ttgctaagct cagtacttcc ttgcctgaag aagtgctaac
    11821 ttttatacag cgaaagcaac aagaaagaaa acgtattatc tatttcgcaa tgggtagttc
    11881 aggcaatccg cgtatgttac gcaaaatttt gttcttctta caaaaacaac cggatctagc
    11941 ggttattgca cctattgact atctgcttga tgacattcaa tctgactcag aagatctttt
    12001 aataacagaa tatctgtcta gtcatctttt gcatgaattc attgattttt cttttatcca
    12061 cggcggggaa gggaccgtcc aaactgcctg tgaatccggc aaaccgttta tcggtgtggg
    12121 gatgcaattt gagcaaagct gtaatattga ctattgtgta aaatacggaa acgcaatcgc
    12181 cctcacaaaa ccagtgactg aaagaaaact gcagattgct ctgcgcgaag tttcttcacc
    12241 caagattcgt cagcaggcac gcacgctgca agccgttttt ccaataaatg gtccacaaca
    12301 agcaattcaa gaaatcgaaa ccttcctgag caaccaccca atatagtttc aagtagaaaa
    12361 agctttttta atagagactc atcagtaatt tcgctattcg agacccagac agtgaaaata
    12421 caaaaaaagt aaatagtgga ttgaaaagtt agagttactt aaattacggt gtctaaaaaa
    12481 cacaaaatca tccttaaaat aaaagatctg acgcaatcca aatgagcata ggtagcgtct
    12541 gccatttatt cttagtcaaa ttctttgaca cctttctctg gttggatcat ctcaacgttt
    12601 cttcaaaaga aagaccataa taattaggca aatacatttt cttaaaatgt gtcatataga
    12661 tctagaagca tcgctttgta gagatttgtg ctgctagtcc cttcaacaat tactaaatca
    12721 aatgtaggat ttaccacttt atctttgata atcaacctcc ctcttctgtt gagacaatga
    12781 tctcgcaatc cgatgcatac tcctatataa actgccagca atccccacat gctgagtaga
    12841 tcatgtcaga atgttctatg tcgtcagcaa cgataaacta aaagtcacgt tcaccatgac
    12901 tgattgctat tcccagcgca attatttcta tacacgtttc atgatttgaa taaacactct
    12961 catcatacat attaccattc atacaccata ctgcagcccc tactatatgg ttgtaagctc
    13021 ccaaatctat atttgccttg atcactttgg tagtagatcg actaattctt aatccattgc
    13081 tgataaaatt ttcatttttg cctccttctt tctgccaaaa cagttaaaac aataataatc
    13141 tttatgtaaa aaagggctgt gacaaaagcc ttgtcacagt cccatattcc gaataaacgg
    13201 tggtcaaaag cttgcatctg cggtaataag ttcaaccaaa cgaaaaataa gccaacattt
    13261 cacaaaaatt taagaagcaa ttttcgtaaa tttcttctta tttcttgaag ctaaccactt
    13321 ctgtcacaac cacttttaga acatttaaag cttcgatgtg acaaaccatc tatgctgctc
    13381 gatcataaaa ttgatagcaa aaattccaca aagtacatat gtcccccatg aaaacaaaac
    13441 attcatatgc ggtacaatga tctttggaag aatcacgatc aaaaatgatg ccagtgccca
    13501 aatccatcga ttgatttgaa tatgatatgt agctagcata ttcaatataa ggtgactagc
    13561 cgttaataaa aagataaaaa caaaaaataa aatcagccaa tacattgttt cccaccttct
    13621 aaaaaccaaa cttagacagt ttggacttcg ttttctccac gactttgttc atctgacagc
    13681 atctttttat cataagcttt tacaaatgga taatagataa taaatgctat caatgtgttg
    13741 atcatgatca agataacaac acgccagtca tttcctgctg aaagataacc gccgattgga
    13801 gctggtaatg tccaagttgg caaaacagta aatctattga ccatttctaa tttgactgca
    13861 aaataactga taatagtcaa aactaaagga actaagttga acgggatgaa catgtaagga
    13921 ttcaacatca taggagctcc aaaaatcatg ggctcattga tattgaagat accaggaata
    13981 atagaaaaac gaccaacctc tttcaagaat tttgatttac agaagaatat aaacaaaaca
    14041 gataccacga tcgttgctcc tgcaccacca attgttaccg tccattgata aaactgttca
    14101 ggagcgatat agggtaattg ggtggcagct gtaccatctg ccaatgcctt tgcattctca
    14161 tccaacatga tcagccacat tggacgaaca acagccccta caatactcat gccatggata
    14221 ccaaatgacc acaacaaatg gatcaaaaac attggtagta aaacaccaag caaattattc
    14281 cctaaaatat tggttaaagg ctcgaataac gataataagg tagcgtttac atcaaagccc
    14341 gcgacgcttc gaatcagcca agtaattgtt aaaatgactg cacctggaat caatgcctca
    14401 aaactacggg caacagcagg tggcacttgt tctggcattt tgatagtaat atttcgattt
    14461 ttaaagaaac gatatacttc cactgcaaaa atcatagata agatccccgc aaacatccca
    14521 gatgctccca aagaactcat aggtaagacc cagccaagag gtgccttttc aggagttaaa
    14581 acgtttacag gaacttgtaa cataaagaac gttcctaaag ataatatacc acctgtcaca
    14641 ctatctaatc catatgattt agctagatcc gaacccataa caaaagttgc ataaatagcc
    14701 atcaatccca tcgtcatcct aaaaggtaaa acaatcgttg ccgaataagg ggcaataaat
    14761 tcattccatc caggaaacgg taaattgata aagaccataa aaaatgtccc aatcaaaatc
    14821 aatgacaatg ttgaaacaac cccattacga attgctaata aatgacgctg tgtaccaatt
    14881 tttgccattg gtgggacaag tttctcttcg acccatttca tcagcttttc cactaaaacc
    14941 actccttttc aataaaatat gaaccattca ttgcatgac
//
LOCUS       DAMEET010000066        14525 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.067, whole genome shotgun sequence.
ACCESSION   DAMEET010000066 DAMEET010000000
VERSION     DAMEET010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.067"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(21..251)
                     /locus_tag="PB780_001897"
     CDS             complement(21..251)
                     /locus_tag="PB780_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733948.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(687..1253)
                     /locus_tag="PB780_001898"
     CDS             complement(687..1253)
                     /locus_tag="PB780_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDE0733949.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1253..2209)
                     /locus_tag="PB780_001899"
     CDS             complement(1253..2209)
                     /locus_tag="PB780_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDE0733950.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2209..2865)
                     /locus_tag="PB780_001900"
     CDS             complement(2209..2865)
                     /locus_tag="PB780_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDE0733951.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3235..5253
                     /locus_tag="PB780_001901"
     CDS             3235..5253
                     /locus_tag="PB780_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDE0733952.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5323..5529
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5680..8094
                     /locus_tag="PB780_001902"
     CDS             5680..8094
                     /locus_tag="PB780_001902"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDE0733953.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8442..9995
                     /locus_tag="PB780_001903"
     CDS             8442..9995
                     /locus_tag="PB780_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDE0733954.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10376..12160)
                     /locus_tag="PB780_001904"
     CDS             complement(10376..12160)
                     /locus_tag="PB780_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDE0733955.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12175..13896)
                     /locus_tag="PB780_001905"
     CDS             complement(12175..13896)
                     /locus_tag="PB780_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDE0733956.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13996..>14525
                     /locus_tag="PB780_001906"
     CDS             13996..>14525
                     /locus_tag="PB780_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDE0733957.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVH"
BASE COUNT         4687 a         2721 c         2851 g         4266 t
ORIGIN      
        1 ctcattaaaa agcataacca ttactccgat ctgtttttaa aattagtttc tacctctaat
       61 atatatcgag tttttatttt gccttcagag aatactgttt ctttttttgc agttacaggt
      121 tgtttatttt cggaaaaagc taatatagct aagattgaaa catccatctg gaatttatct
      181 tttttactgc tttgatcata aaagtctgca tattcatcac tgatattttt tctaataaat
      241 tcttccatca taaaaatcac ctcaaaacga ttatacgaac ttacgttctt gttgtaaagc
      301 gatatttgag gggcaaaaaa ggggcataat ttgactgata ttaagttagc tattgatata
      361 tgaatgacat acaaaaatat aaaatctttt aaaaatgctt atatatcaat gtttttgcca
      421 ttcttttata tatctgttat atgtcaaaaa aatgtgttca atttcaattt gtatttgaat
      481 aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc
      541 ttctcatgtt tttgatttgg ttggacttat taccgcagat ataagctttt gagcaccgtg
      601 aataaagagg ttatgaaatc aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca
      661 tatcatttat gttttgcgtt ttttatttat ctttttttct caatacaata taaaataggc
      721 ggctggttct tttggttgat aaaccgataa ttcagcacat tatatgtttc ctgtggcagt
      781 ttggaacaaa aagaatcaac catatccagt tctttttctc caccttcatg accatagtag
      841 acgacaagga tcaatcgtcc gcgaggcaca agtcgtttca atatttcttc catagctgtg
      901 cgtgtagtct ctggcatagt gatgattgct ttatcacttt ttgggagata ccctagattg
      961 aaaatccctg ctttgagatt ttgttgttca gctatcactt ctcctaacgt ttcgtggcct
     1021 tgtagaaaca atgagacacg ttcttctaac tgacgctgtt ctagcctttc acgagtagca
     1081 tcgattgctt gttgctggat atcaaaacta tagacatgac cagtttttcc gatgtgttct
     1141 gctagaaaaa ccgtatcgtg gccattgccc atcgttgcat ctacgacgtg atcccctggt
     1201 tgcaagattt cttgcagtaa tgtatggctg aaatgcaaag cagtttgcaa catcagacgc
     1261 tcaccttttt ccgtacatca tattttcctt ggaagctatc tctgcgaaga agttcttcat
     1321 caattgcatt caagacttcc cattttttca agctccacat tggtccaatc aaggaatccc
     1381 acggcgcatc ccctgtcaaa cgatggatga tgatctcttt tggtatcatt tccagctggt
     1441 cgcaaatgac attgacatag tcaggacggc tcattaattg aaggcgacct tcatgatagt
     1501 cccgcaacat tctcgttttg cgcatcagat gcatcaaatg gagcttgatt ccttggatat
     1561 ctgaatctaa aatggtgcgt ctgacatttt ctttcatcat ttctaaactt tcgcctggta
     1621 atccgttgat caaatgcgta caaacacgga tattatgttt gcgtaattta gccacgccat
     1681 ctaaataagt ttgataatca tgtgctcggt tgatcagttt tgaagtttct tcaaaagttg
     1741 tttgcaatcc aagttctacc cataaataca tccgctcgtt cagttcagca aggtaatcaa
     1801 ctacttcatc tggcagacaa tccggtcgtg taccgactga taatcctacg acacctggta
     1861 aattgactac ttgctcaaat cgttctttga ttatttcaac tggcgcatgg gtgtttgtaa
     1921 aattttggaa ataaacgatg tattgttgga catgaggcca tttttgatgc atcatgtcga
     1981 tttctttctg gaattggatt ggaagagggt cttcaggtgc aacgatcatg tctcccgaac
     2041 cagatacact gcaaaaagta caaccgccat gggcaacagt cccatctcga ttcggacagt
     2101 caaatcctcc atcgatcggc accttgaaaa ttttcccgcc aaactcattt cgtaaagcgt
     2161 aattccaaga atgatatctt ttatttcctt ctgaataagg aaaagtcatt aaaagtcctt
     2221 ctttctgtaa gagttatcta tttgttttct tttttctcta tacactggat aagtcaggca
     2281 cagccaagaa agactgagta agtagccgcc gatgatatca gtaggaaaat gaacgcccag
     2341 ataaatgcgg cttatcccga cacttactat aataaacatc atcagcaatt gaagcaatgt
     2401 gcgtaaggtt cttttttgaa caaaagctgg aagcagaaat agtaaagaac cgtaaagaat
     2461 catactgcct gctgcatgtc cactagggaa actgtaactt gtttcaacta ctaagtgcgc
     2521 caatgaagga cgctctcgca tgaaaaatag tttgatcacc gggttgatga ttccagcaat
     2581 tacaataaat ccagaaacga accaaaatgt ttctgccctt tttttcttat accacatcca
     2641 taaagccgca caagcaaata aaatgatgac ggtaagagga ttaccgaatt tagtcataaa
     2701 gagttggtag ctgttcagat caggataaga agctctgaca atgcctgtaa accactgatc
     2761 gaaaccagaa agccacgaca gatggaactt tacgatccag cccaaaatga aaaacaggac
     2821 aagaaagaag ctaccagcca cctgaaatga tgttttatat ttcatacaat tcctcctttg
     2881 aagcaatgac ttattatagc atttttggtc cagcgaaaaa aacggtaaag agataaaacc
     2941 aatgtatgat tcctctttga ataaccaata ttcttataaa aaaaataact gtaatcggaa
     3001 agacattttt tggtaatgca aatgaaatgt tagggaaata aaatcagtgt ttttcgaaat
     3061 ccttttaaac aagaacgtag acgtaggaag aacttgaaaa tgaacagaag ttgttcatta
     3121 cggtaaatta caatatgatt acaaaaaaca acaaagctct taaataactt ttaataaata
     3181 ttaagagaag ttataattat taataataga taaattgaag ttggaggata gaagatggaa
     3241 aacattgcaa gaaaagaacg gagacgttta gaacagacca aacgttttcg aaaagcaaaa
     3301 cgaggggcag caatcgtggg aactgcgatg gtaggctgtt ctgtcgctgc cccattagta
     3361 cagccggttc ctgttcaagc ggatgaaaca cctgttcagt tcggcgcaag aatcaataca
     3421 gcggcattta tcacagaaat tgctacttat gctcaaccaa tcgctcaagc caacgatcta
     3481 tatgcatcag tgatgatcgc acaagcagtt gtagaaagcg gttggggcag tagtgcgtta
     3541 tctcaagcac cttattataa tctttttggg ataaaaggca gctatcaagg acaaaccgtc
     3601 tacatggata cgcttgaatt tttaaataat caatgggtaa ataaaaagga gcctttccga
     3661 caatatcctt cttttgctga atctttcaat gacaatgcct atgtacttag aaatacttca
     3721 tttggcaacg gttattacta tgccggtact tggaagagca acacgaaatc ctatacagat
     3781 gcgacagctt gtttgacagg gcgttatgcg actgatcctg gttacgcagg caaattaaat
     3841 aatatcatcg caacttacgg attgacccaa tatgatacac cagctagcgg aaacgcaggc
     3901 ggtggagcga ctacaggtgg caataatact ggaagtgcag gtagtactgg cggttcaagc
     3961 aattctggta atacaaactc aggttcagca acttcgacca cttatactgt ccaatctggt
     4021 gattctgttt ggggaatctc aaataaattc ggtatcacaa tggatcaatt gatccaatgg
     4081 aacaacatcc aaaacaattt catttatcca ggacaaaaac tgacaatcaa aggtagccag
     4141 gaaaatggct cttcgacgaa taattccggt aacaatacca actcttcagg aaatgctggt
     4201 acatcaaacg gaggacaaac aactggtgcg aaatacactg ttcaatctgg tgattctgtt
     4261 tggaagatct caaatgatca cggaattacg atggctcaat tgattgaatg gaacaatatc
     4321 aagaacaatt tcgtttatcc aggacaacag ctaatcgtat caaatggcgg ttcttctaat
     4381 gctggagcaa atactggctc aaatactggg aatacaagtg gctctagttc gtccagctca
     4441 agttcttcta atacagcttc aggaacgaaa tatacagtta aagccggaga ctctgtttgg
     4501 agcgtctcaa ataaatacgg aattacgatg gctcagctaa tccaatggaa caatatccaa
     4561 aataacttta tctacccagg acaacaactg actgtttcaa acagtggatc aacaaacagc
     4621 acgtcaggta atacaggttc gtcaaccaat acaaataaca gcagttcagt taatactggc
     4681 agcagttcgt ctcaaggcac ttataccgtg aaagcaggtg aatctgtttg gagcgtggca
     4741 aataaaaacg gcatcacgat ggatcagtta atcgaatgga ataatatcaa aaacaacttc
     4801 atttatccag gacagcaatt gatcgttaaa ggtggaactt cggcaaatac aaatacaggt
     4861 tcaactactt ctgcagcgaa accaaataca ccgaatacgt ctgctacgac aagtacatca
     4921 tccactggta atacgatgta taccgtgaaa gcgggcgagt cagtttggag cgtagcgaac
     4981 aaacatcata tcacgatgga tcagttaatc gaatggaata atatcaaaaa taatttcatc
     5041 tatccaggac agcaagtgat tgtgaagaaa ggttcatctc aatcaacaaa ccaacaatca
     5101 gttactggtt cgaaaaccta tactgtgaaa tctggagaat ctgtttggag tgttgctgac
     5161 agtcatggaa ttacaatggc tcaactaatc gaatggaaca atattaagaa caactttatt
     5221 tatccaggtc aaacgttgat tgtagctaaa taaaatagct gttcgaagaa ataacgattg
     5281 aattccacaa aaatatttgt tactataagg ctacttaaat atgacaacac ttgaaaagga
     5341 agtagtaaaa agactgaagt ttcagagagc gcatggctgg tgagaatgtg cacggatgct
     5401 tttgaactcg cctttgagtt gcccttgctg aatacccgag taagcaagcg gcggtgacgg
     5461 ccgttacaac gttcgaggcc ttttcactaa ggtaaaatta ggtggtaccg cgtagattac
     5521 gtcctataaa aagagcaatc tttttatagg atttttttat ttcttttgtg aagtttgaga
     5581 aaacaaagca aaaggaaata aaaataaaca gattctgccg gttttttcgt tatatttata
     5641 atagaaacaa gtgttttcaa tagaacagga ggacagcaaa tgagttacaa tcataaagaa
     5701 attgagaaaa aatggcaaaa atactgggca aaaacgaata ctttcaacac ccatgatgat
     5761 cctgaaaagc caaaatttta cgcactggat atgtttccct atccatccgg tcaagggcta
     5821 catgtagggc atcctgaagg ttatacggca acggatatcc tttcacgtgt gaaaagaagt
     5881 caaggataca acgtgctgca tccaatgggg tgggatgcat tcggtctgcc tgctgaacaa
     5941 tatgcgctag atacaggaaa cgatccagct gagtttaccc aaaaaaatat cgaaacattc
     6001 cgacgccaaa tcaattcttt aggatttagt tatgactgga atcgcgaagt gaatacaact
     6061 gatccggagt attataaatg gacacaatgg atctttacga agctttatga aaaaggtcta
     6121 gcttatgaag cagaagtagc ggtaaactgg gtacctgaat taggaacagt tatttcaaat
     6181 gaagaagtga tcgatgggaa aagtgaacgt ggtggctatg atgtagtcag aaaaccaatg
     6241 cgccaatgga tgctgaaaat cactgcttat gctgatcgct tattggatga tctagaatta
     6301 gttgattggc cagaaagcat taaagaaatg caaagaaatt ggatcggtcg ttcagtagga
     6361 gcaaatattg aatttaaagt ggctggtacg gataaatcct ataccgtatt tacgacacga
     6421 ccagatactc tattcggtgc aacttactcg gttcttgcac ctgaactaga tcttgttaga
     6481 gaaattacga cccctgaaca aaaagctgct gtagaagctt atatcgaaga gacagctaaa
     6541 aaatcagatc taaagagaac ggatcttgca aaagaaaaaa caggagtatt tactggtgct
     6601 tatgcaatca atccagttaa tggcaaggaa atcccaatct ggatcgccga ttatgtatta
     6661 tcttcatacg gcacaggagc catcatggct gttccagcac atgatgaacg tgactttgaa
     6721 ttcgctcaaa ctttcggact agaaattctt ccagttatcg aaggtggaga tgttgaaaaa
     6781 gctgcttaca cagaagatgg tacgcacatc aactctgaat ttctagacgg catgaacaaa
     6841 caagaagcaa tcgataaaat gaatgaatgg ctagaagaaa atggcgtagg gaaaaaagaa
     6901 gtcagctatc gcttacgtga ctggctattc tcccgtcaac gttactgggg tgaaccaatc
     6961 ccaatcatcc attgggagga cggaacagtc acagctgtcc cagaagaaga gctaccatta
     7021 cggttgccta agacaaaaaa catcaagcca agcggtactg gagaatctcc attagcaaat
     7081 atcgaggagt gggtcaatgt cgttgatcct gtaacaggta aaaaaggccg tcgcgaaacg
     7141 aatacaatgc cacaatgggc aggaagctca tggtattacc tgcgttatat cgaccctcat
     7201 aacaaaaaag aactagcaga ttatgaaaag ttgaagcgtt ggctaccagt tgacatctac
     7261 attggtggag cagaacatgc ggtacttcat ttactatatg cacgattctg gcataaattc
     7321 ttatacgata tcggtgttgt tcctacaaaa gaaccattcc aaaaactata caatcaagga
     7381 atgatcttag gcgaaaacaa cgaaaaaatg tcaaaatctc gtggtaatgt agtcaatcct
     7441 gatgatgtgg tcgaaactta cggtgcagat acactgcgca tgtacgaaat gttcatgggg
     7501 ccattagatg cttcgattgc ttggagcgaa aatggcttag aaggaagccg taaattcttg
     7561 gatcgtgtat ggcgtttgat cgttgatgag aacaataaaa tgcgtgaccg tatcactaca
     7621 ttgaatgatg gcaagcttga taaagtgtat catcaaacag tgaaaaaagt gacagaagac
     7681 tatgaaaatc ttcatttcaa tacagcaatc tcccaattga tggtctttat caatgaagcc
     7741 tataaagtgg acgctctgcc ttatgaatat atcgaaggat ttgttcaact actcgcaccg
     7801 attgctcctc acatgggtga agaactatgg gcaatccttg gaaatgatgg tggtatttct
     7861 tacgcaccat ggccaacata cgatgaagca gcacttgttg aagacgaagt agaagttgtt
     7921 ttccaagtaa acggtaaagt tcgtgcgaaa agcaatgttc cccgagactt aggaaaagat
     7981 gagctagaaa aagcagcatt agccaatgaa atagtgcaag aatatattga aggcaaaaca
     8041 gtcagaaaag ttattgcggt accaaacaag ttagtcaata tcgttgctaa ttaattggct
     8101 ctttgtcaat aaggactgat gagttgtgca aaatcaaatc tgggccagaa tgaacttcat
     8161 tctgtctcag attttttgtt atttcaaatt gattaattga ttgatcaaaa aggttttgat
     8221 tttcatgttc tcaaacacgt tcaatgtctt tttcttctga atggtcttgt aagagatctc
     8281 tgtaatcatc aaatttttgt ctattaatct tagtattttt tgatgtataa actacctgaa
     8341 attgagagta cataccaaat tcataaattt tttattagtg ggactagtga ttgtatggat
     8401 tttgacgcat aatgaatgaa gtatgagaaa aggagagaca aatggatcaa gaactattta
     8461 ttatcgaagt attatcagct aaacaaaaaa ttatcgagaa agcgaaaaaa agagcgaaaa
     8521 gaataatgtt caagctttta agcttagtat ttttctctgg tttgcttttt ttcggagctg
     8581 tatcgttcct atatcgccat atgagtgcag tagtccgttc agctgaaggg atgcaaaaat
     8641 taaataattt gtatcacttt tcgtggggag ttggcggaat aacaattgtt gtgcttattg
     8701 tttttcttct acttatttgg ctccgttacc atcaattgtc gcgtcatttc gatcaatggc
     8761 ttagtcaaag agcacaacag tttttacata ataaaccaac ttctcaagat gatgactatc
     8821 tttattttga gaagacttcg acgagtcaag cttttcggat acaaaaaagt gattgccagt
     8881 tattgctgag atcacttgat gatttaccta tctacttagg cgaaagcaaa tcaaaattcg
     8941 gaatgaacac attccatatt tttttactag aaaacgaacc cagatgggaa aaaagccagt
     9001 cttcgcctgg tggatacctg acaactaaga tgagtacatt agtcactggt tttttcctca
     9061 ttcttttagc tggaacaatc tattatggga gatacactca gccaaaaatg tcttctcata
     9121 taaatgctgc ttctcacatg acaatgaact caactaacga tacgcatata caaaagacaa
     9181 atgaagggtt gttagctgat cctaagcaaa aggtaaattt gacaacacaa aagataaatg
     9241 atctcaaact tgatacagag acgcactctc tctatatgac aacagatagt ggtgctaatt
     9301 gggtatttgt gccgattgat tcgcaatggt tgcgttttgg cgaatacacc ctgacaacgg
     9361 ggctgattcc tgaaggttat tggatggaca acacatatga tatttccgcc gatttctcat
     9421 ggtttatcta ttcttctggg gatgatgtac gtctgctgac ttcaagagat aatggtaaaa
     9481 catggcaaac gtctattatc acccaatcag ttcagccgat acgttatcga aaagcggtat
     9541 tttatggcaa cgatggtcta tttatctgta catcagccag tgggatgtct gcagaaactt
     9601 tgtacattta cactactatg gatggcggaa tcacttggat aaaaagtgga agtacaatga
     9661 tcaatcagcc agtacaaaat gtcagcttcg ttacaacttc tttaggtttt gtttcgacac
     9721 gtgaaagttt gttttataca aacaacggag gacgctcatt caaagaatca gttctgtcga
     9781 ttccggaaaa ctatcagaca ggggggatag atctttttca atcaccaaat gaagttactc
     9841 aaataagcgc gaatacgtta gagacaaaat tttatttgtt gaaaaaagga ccaatcgatg
     9901 cagggaaaat gtttgcctgc ctcttccgtt caactgataa cggggaaaca tggcaattcg
     9961 ttcagcaact atcgcaagta gaacaaggag attaaagaag agagattgtg agcctgacgt
    10021 tcagcaccga gtcacaagaa acaaattcaa aaaacagctt ctcatatttg ttgaatagtg
    10081 taactcgtga ttcggagttg acaagagcaa caagaagcga ttttgaagta tagcttctcg
    10141 tattttacaa gatcgtgtct tattgccgat aggttgtctc ttgaacaccg tttattcgta
    10201 aaaagggatt gtgacaagtc ttttgtcact acttaccgaa cgaaactgaa ggagctatcc
    10261 aaaaagtatc attagtatta ttctttgtgt ttctagtggc accactagta catttaaaaa
    10321 agctatcgat catggactaa tcatacatga tcgatagctt tttagataat gtttgttacg
    10381 ctatctgaaa ttgactatta taaagactgg cataaagcgt tggtgattgg agtagttcag
    10441 aatgtgttcc tttttcgacg atatcgccat tcttcattac aaggatcatg tctgcatttt
    10501 caatcgttga caaacggtgt gcaatcacaa aacttgtcct atcttttgtc acagcttcca
    10561 ttgcttgctg aatcaatgct tctgttcgtg tatctacgct agaagttgct tcatctaaaa
    10621 taacgacagg aggattcgct agaatgactc gagcaattgt caataattgt tgctgtcctt
    10681 gagataacgt gccgttttcg ctagaaatga tcgtatcata tccgtctggc agtgttcgaa
    10741 taaagtgatc acattgtgcg atttcagccg cttcgataat ttgttctctt gtggcatcca
    10801 ttttcccgta ggcgatatta tcagctaccg ttccttcaaa cagccaggta ttttgtaaaa
    10861 ccatcccaaa agaacgacgt agttctccac gtgaaagctt ggtaatatca agaccgtcga
    10921 attcgatctt tcctttattc agttcataaa aacgcatcag cagattgact aaagtcgttt
    10981 ttcctgcacc agttggacca acgatcgcta ccgtttgttt tggctttact gaaaaatcga
    11041 cttgattcat caatatttta tctgatgtgt aaccaaactg tacatttttg aaagctattt
    11101 ttcctttcgg ttgttctaaa agtcttgcat ctggaaggtc tggaatttca tctgtttcat
    11161 ctaatatctc aaatatccta tcgattgctg ccattgcgga ttggatcgag ttgatcacat
    11221 aagaagcggt agaaattggt tcagaaatct gattgatgta ctgaagataa gcttgtaaga
    11281 agccgatcgt cacaccacct gatagaacaa gcatagcacc gaataccgca cttatgatga
    11341 aggcgagttg attaatgaaa cgaatggccg gatagatagc acagttcata aattgagcac
    11401 gtttaaaagc gttgtattgc tttttattta cttcgtgtat agcagcttct gcatgagctt
    11461 gctgattgaa tgttttgacc actagattac cagctaagat ttcttccatt cgattattta
    11521 aaacacccag ttcatactga ttctgttcag aaatctgttt gtttttgttc gcaatcttag
    11581 ttgtaacaat tgaactgcca acgatcagca ataagacgag caaagtaagc ttgctttcga
    11641 tataaaacag catcagacca gcagcagcta ttgttacgac agaagtaaaa aattgattga
    11701 tacctgtcaa taacacttga gaaagttgat tgagaccggt agttgtccga ctaatgatgt
    11761 cacctacttg atgattgtca aaaaacgaca tcggtaatgt tttgaatttt tttgtgattt
    11821 cttttcgcac cctcaatgtg atcttttcgc ttaacgctgc cattgtccgt tcctgaataa
    11881 atgaagtgat gctgcttaag actgaacagc cgatcagcaa taagacaggg aacaacaatg
    11941 cttgtttgat atcactgaaa gaaagctgtc caatacctgt ttttcttatt tctgataaga
    12001 gatcatcgat cccgattccc atgatcaagg gcatggctac gactaatagg ttcccgaata
    12061 aactgcatag aaacaatccg taaaaatatt tatgttcagg tttgatcatt ttccaaaaac
    12121 gtatcaaagc acgcgtgttc aacgtttttt tcttttccat cgtatccctt ctttctatat
    12181 tccttgtgat tttgcaaact cctgataagt ttggcaatta gacagtagtt cttcatgagt
    12241 accttgtcca acgatttttc cttcatctaa aacaatgatg ttatctgctt gttggattgt
    12301 actgaggcgt tgagcaacaa tcacgagagt tttctccgac atgtgtttat gaagggctgc
    12361 tcttaatgct gcatctgttt tatagtctaa agcggagaaa ctatcatcga atacatagat
    12421 ctctgctggt ttgatcaacg ctcgagcaat acatatccgc tgtttctggc cccctgaata
    12481 attagaaccg ccttgagcaa caaagctgtc caatccgtca ggaagggtgt ccactagttc
    12541 ttctaattga gcgatcttga tcgctttaga caactcttct gttgaggcat attcatttcc
    12601 catctgcaag ttgcttaaaa ttgtcccgct gaataagaaa gccttctgag ggacataact
    12661 gatgtgggag cggatctgtt cttgagtcat ttgagtaaga ggaataccac caagggttac
    12721 ttttcctatc gtagggtcac taagtctcaa gagaagtttc gccaaactac tttttccaga
    12781 tccggtcccg ccaacgatcg ctgttgtttt tcctttaggt attgtgaaat caatatcttc
    12841 tagaactgga tcagagattt cagtatattt aaatgtgaca tgttcaaaag cagctgcatt
    12901 tgtctgatca aaggttggtt cagctactgt tttgatcgtc tcttcctcaa tttcgatctc
    12961 tgcttgcagt atttcttcta tccgtcgtaa tgaggcaaac gatttaggga taatgaccaa
    13021 taccatagct gcgatcatca agtaagcaag tgtcagcata gagtattcga ctacagcagt
    13081 cacgacaccg atttgtaatg ctccttctgc tacaagattt ccgccaaacc acaaaataga
    13141 agcataaacg attcccatga gcaaaaaagc aattggagtg acccaagcaa atattcggtt
    13201 cactttgatc atttgattag catagtcagc aaagctctta ttcgttcgct tttcttcata
    13261 tgattgatta tcaaaggcac ggatcatatt aatcccagta aagaattctc tcaatgtgat
    13321 ggtgatccga tccatttttg gctggatact caaagataaa ggtgcccctc gtttcatcag
    13381 tcgataaaca atccatacat aaaaaacaac agagatcaat gtaacccatg ttagtaatag
    13441 agaataacga aacatcatga gaatggtgaa aacgcaaata accggtgctg gtaagatcat
    13501 ttggcaaaat aaaacaatca tttgctggac attatcgaca tcattcgtca ttcttgtcaa
    13561 aagagatcca gaactaaaac gactgatatc cttcattgac agctgttgga atttacggaa
    13621 atatttctcc cgcgtttgga atccaaacct agcggaagcc aatgctgaaa agtaactaga
    13681 ggcaactgca gcgattgttc ctagaaacgc tacaagcagc atttcaactc caattttttt
    13741 tataactgag gcttcgccac ttgctatacc tgtgtcgatc agctttgcta ctaattttgg
    13801 tacacctaat gtaccggcga tttctaaaca gatcatcaca aaggtaagga gagcgagtcc
    13861 tttgtttttc tgtaagaatg tttttaagat agacatatta ttcctccttt tttcctgata
    13921 cttttcgatt ctataccttc aagtaacttg aaggtcaata gaaaaaagaa taaactgaaa
    13981 aagaggtgag aaaaaatgaa aaaaatgtat acgattggcc aagtatcgga actatttgat
    14041 ttgccaaaat caacgatccg ctattgggat gaacaaggat taatccgttc aagtagacaa
    14101 gaagagaatg attatcgatt gtttgatatc gatgatattt ttatgcttta tgatatcgtt
    14161 ttttatagaa aattagatat accgatcaaa cagatgaaga atttatatgg aaaaacattg
    14221 acggagttat atgagacact tgatgaaaca gaaagaagaa tccataaaga actggtggtg
    14281 atgaaacaaa aacaaaaaga gataagggag caaaaaaaac agttgaaatt gatgatcgat
    14341 acaaacgagg aagaatttcc tgtagaagag atcccttttg actgtatgat ctcaacggag
    14401 tttgaagata ttgtggaaat caaaaaattt ctacctaact attcaagctt tgggatgatg
    14461 agtatgccag cttccagttc acagacgatg tatggatttt ttatcgaccc tagtgaagta
    14521 cattt
//
LOCUS       DAMEET010000067        14119 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.068, whole genome shotgun sequence.
ACCESSION   DAMEET010000067 DAMEET010000000
VERSION     DAMEET010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.068"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..556)
                     /locus_tag="PB780_001907"
     CDS             complement(<1..556)
                     /locus_tag="PB780_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HDE0733958.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGA"
     gene            complement(572..1051)
                     /locus_tag="PB780_001908"
     CDS             complement(572..1051)
                     /locus_tag="PB780_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDE0733959.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(1057..2081)
                     /locus_tag="PB780_001909"
                     /pseudo
     CDS             complement(1057..2081)
                     /locus_tag="PB780_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            complement(2098..3204)
                     /locus_tag="PB780_001910"
     CDS             complement(2098..3204)
                     /locus_tag="PB780_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDE0733960.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(3433..6030)
                     /gene="adhE"
                     /locus_tag="PB780_001911"
                     /gene_synonym="adhC"
     CDS             complement(3433..6030)
                     /gene="adhE"
                     /locus_tag="PB780_001911"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDE0733961.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(6336..6716)
                     /gene="yajC"
                     /locus_tag="PB780_001912"
     CDS             complement(6336..6716)
                     /gene="yajC"
                     /locus_tag="PB780_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDE0733962.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(6813..7958)
                     /gene="tgt"
                     /locus_tag="PB780_001913"
     CDS             complement(6813..7958)
                     /gene="tgt"
                     /locus_tag="PB780_001913"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDE0733963.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            8176..8874
                     /locus_tag="PB780_001914"
     CDS             8176..8874
                     /locus_tag="PB780_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDE0733964.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(9042..11681)
                     /locus_tag="PB780_001915"
     CDS             complement(9042..11681)
                     /locus_tag="PB780_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDE0733965.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(12300..13331)
                     /gene="queA"
                     /locus_tag="PB780_001916"
     CDS             complement(12300..13331)
                     /gene="queA"
                     /locus_tag="PB780_001916"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDE0733966.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            13524..>14119
                     /locus_tag="PB780_001917"
     CDS             13524..>14119
                     /locus_tag="PB780_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDE0733967.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEA"
BASE COUNT         3959 a         3095 c         2543 g         4522 t
ORIGIN      
        1 aagcgcctac gagataacct cccataaaca aagcagagat cgaagcacca ccagcaacaa
       61 gtgaatacat gataaaagca gtacttggag gaatgaccat acctgtcgga gctgacgcga
      121 tattgaccgc tgtcgcaaac ttccggtcat atccttcttc cacttgctga gggatcaaca
      181 ctccaccaat cgcggtagaa gcggcaatgg ccgagctaga gatggaacca aataaagcat
      241 tccctaacac attcgtatga gccaaagccc ccggcacgcg cccgacgaac aacatcgcaa
      301 aatccactag tttcttagct atccctccat tattcatgat aatgcccgag agaacgaaaa
      361 acggaatagc gatcagcgaa aaactattga tacttcccac cattttttga gctgaactaa
      421 aagcagaaac atcaaacggt ataactaaca ataaagtcat catcgatgag aatgcaatac
      481 ttatagcaat cggcatccct aatacaagca acacaacaaa tacaaaaaat aacactaatc
      541 cggcttctat tgccatttta ttcctccatt tttatatttt ttcttgaatt gctggcttgt
      601 tgcttaatgt atcgattaga ttgatcaggc tatagataat gatgagtacc cccgatacag
      661 gtactgacag ataaacatat cccataggaa tacttaaaga aggcgagatc tgccccattg
      721 tcaatgtgac agcattccat ccgccataca ccatgatcat cgcagcaaat accagaaata
      781 aactttgaac aacaacttct aaaattttac tttttttgcc agttaatttt tcacttaaaa
      841 ccgttacggc taaacgtgct ttttgtccca caacatacgc ggaagacaac atggcaaacc
      901 agacaagacc gaatcgaaca agctcttccg tgaccgtatt tggatttcct aaaaccgttc
      961 tggaaaatac ttgccataaa acaacagcga ccatcccaac aagcaaaaca gctcccagga
     1021 tctctaatac cttgtttaaa atcattttca ttacagctac gctccttgct cttggatctt
     1081 ttgataaagg tctttgtagg taggatcgtt tttgaatata tcatgaagtg gggcaactgc
     1141 ctctcgaaac ggtgtattat ctacatcatt gaattggact ccatgttctt cttgtgctaa
     1201 agctttttct tcttcaattg cttttgcaaa agcttcaatt tcaaaaaccg ttgattcttt
     1261 cgctgcttct tcaatggctt tttcctgttc atcagttagc tgttctttga tggcatcatt
     1321 catgatgatg atatctggca cacgtgtatg ctcatcatag gaatagtatt tagctacccc
     1381 gccatgtcca gctgtataca agacaaattc attgttttct gatccgttga tcaaattgga
     1441 ttgaagagac gtgtacactt catcacttcc cataggtacg ggcgaaccgc ctaaaagctc
     1501 taccattttg atagcggttt cgctttgcat cacacggatc ttctttccct tcaaatcttc
     1561 cggcgtatgg atcggaccgt ctaccatata aaagctgcgc tgaccagaat cataataggt
     1621 caatccggta aatcctaaat ttttggtcga ttggtagatt ggctccatta tttcttcatt
     1681 atccatcacc ttaaagaaat gttcttcatc attgaataga taaggaacgg aaaaaatcga
     1741 ataatcttta gagaaacttt ccaacgctga ggcacttact tttgtgaaat ctactgctcc
     1801 tgactgtgtc aattcgatca gttcacgttc tccacccagt tgtccatctg ggaaatactg
     1861 gatctgtacg gaaccatctg ttttttcttt tactaaacgc ccaaattcat tcattgcatc
     1921 aattactggc tgttgcttgc ataagcaaag cgcaaggtaa ctatctcatt tacgtctgca
     1981 tctgcgacag ttgccacact agaattattt ccgcagccgc ttagacctag cagacctagg
     2041 ctaagcgaac ttgctgcaaa cagcagtgat gtttttttca tatgggtttc ctttctttta
     2101 acaataagag acgagcaatt ctgtcaaaca aagcaccgtt aacgactgtc cataaggcat
     2161 tgctgtgatt ccgatattcc gataaaaatc taaactgtcc cccattcctg tcccgattga
     2221 aacgttttga acttctcctt tttcatcgat ttgttcaagc aatccttgga tagcacgata
     2281 agcgacttct ttatattctt gtggtaaata acgtttatgg accgctttca aaataccgta
     2341 agcaaatcct gcagttgcag aagattctac gtaagaagaa ggatcatcca acaacgtatg
     2401 ccataaaccg ctttcagact gataagtttt taatgcacgc acttgtgctt caagtgtttc
     2461 gatcaataat ttgcgcaatg catcttcttt cgataattct aagatttcaa tgatctctgg
     2521 aatagcaatc gtcaaccagc agtttccacg agcccaaaat gcttcggcat agttatgatt
     2581 tccttcaaac gtccagccgt gataccacaa tcctgttcgt ttatccatca agtatttgat
     2641 atggatcata aactggtaac gagcttcttc taaatattct gggcgattca acagtttccc
     2701 gatttttgcc aaaggaagca ctgtcatcat caatgtatcg tcccacaact gattcttatt
     2761 ttccggtcca tatgtggcat gctgtaaccc gtcttcattt gtccgaggca tgtcattcat
     2821 gacccattct gcccattgtt ctaaataagg caaatagcgt gaatctttcg tgtcttcata
     2881 caagtaagcc atagtcaata gcggtgccat tgtattgacg tttttgggcg gagcgccttc
     2941 ttccatacga tcctcaaacc attcattgac cacttgataa gcgcgaggat tttttgtcat
     3001 ctggtaattt ttataaatac catatagacc gacaccttgt ggccaattcc acactgtcca
     3061 gcttttgtca tctactttca gtccatcaaa gtccaacaaa aattcaccat tatcgtcttt
     3121 gatcgttgtc aggttttcaa cgagcaggtc aattttttct ttcactaatt ctttaggtac
     3181 aaaaatctct tctttaataa tcatctttac gccacctttt taaacgcttt cattttatat
     3241 ccttaatata gcagtaatac cgtccactat aaatatcgct ttttgaacaa aaactgtcta
     3301 aaattgacct aaatgttaaa taacaaaagt aactttcagt tacttggaaa ttaaaaaaag
     3361 aacttggaga aacaacgttt tccaagttct ttttcatcta ttttcttatc ttttttctac
     3421 gctttctttt gcttatccgt tgtaagcatc aatgatgatc tgtttcaatt cagagatcaa
     3481 tggttctttc ggattagctg tcgtacattg gtcttcgtaa gctaattcag ccatacggtc
     3541 tacggttgta tctagtgttt cttgtgtgac tccttgagct tttagactca tgttgatgcc
     3601 gacagatttt cctaattcat aaacagccgt tgctaaagct tcgactaatt cctctgttgt
     3661 ttcgcctttc agacctaaga attttgcgat atctgcgtaa tctgtatctg cacggaaata
     3721 gtcatattta gggaacatcg catgtttttg tggatctttt gcattatagc ggataatatg
     3781 cggtaaaaga atcgcatttg tccgtccatg agggatcccg tattccccgc cgattttatg
     3841 tgcgactgag tgacaaatac ctaggaatgc gttagcaaac gccataccag ccattgtaga
     3901 agcgttgtgc attttttcac gtgattccat atcaggtgtt tttactgatt tttctaaata
     3961 gtcaaagact aacttgattg cttgtaggct caatccacgt gtataatctg aagccatgac
     4021 tgaaacatag gattcgattg catgtgttaa cacatccatc ccggtatctg ctgtgacaga
     4081 tgctggaact gacatgacga attgaggatc gacgattgcg acatctggcg tcaatgcata
     4141 atctgctaac ggatatttca catgtgtttc actgtcagtg atgaccgcaa atggcgttac
     4201 ttcagaacca gtccctgatg ttgtcgggat acatacgaat tgtgtttttt ctggtttgtc
     4261 gatcttatat gtccgtttac ggatatccaa gaatttttgt tttgcaccaa agaaagatgt
     4321 gtctgggtgt tcatagaaca tccacattcc cttagctgcg tccattgcag aaccgcctcc
     4381 aagagcgatt actgtatctg gcttgaagtc tgccatcagt ttcgttcctg cgtatacggt
     4441 atttgtcgat gggttcggtt caacatctga gaagacttcg atcttcacat catttttacg
     4501 tcgtgccaat acttcacgaa ctgtatcgca gtaaccgaat tggaccatac cagggtcaca
     4561 aacgatcatg acacgttcaa cgttttccat tttctctaag tacaatagag agttcttttc
     4621 aaagaagatt tttggcggta atttgaacca ttgcatgtta tttctccgtt tcgctacagt
     4681 tttgacattg attaagttta cagcagagac gttacgagaa acagagttct taccataaga
     4741 accgcatccc aaagtcaatg acggaatcat ttcgttatag atatcaccga tgccgccttc
     4801 tgcagatggt gtattcacta aaatacgaca agctttcatt cgaagaccga atttgacatg
     4861 caactcttcg ttttctgaat ggataactgc tgtatgtcct aatcctccaa gattcaacat
     4921 accttcacac aaatcaaacg catggtccgt gttgttagct ttgatcatgg caagaactgg
     4981 agaaagtttt tctcttgata gcggataatc tgggcctacg ccttccaatt cagcaatcag
     5041 cattttcgtt ccttttggta cactgattcc tgccaatttt gcgatttcca tcgcagaatg
     5101 tccaacgatt gatggattga ccgcatattt tccttcgttc atcactgcat cttctaattt
     5161 ttgcaattca tctggtttta cgatataaac ttggtgtgct tggaattctg cttttactgc
     5221 tgcatagatt tctttatcca cgatcacagc ttgttcagaa gcacaaatca ttccattatc
     5281 aaatgttttc gaaacgatca aatcgttcac tgcacgttta atttttgctg ttttttcaat
     5341 ataagctggt acgttacctg gacctacacc taaagctggt tttcctgtag agtaagcaga
     5401 cttgaccatt ccagctccac ctgtcgctaa gacgatcgcg atacctggat ggttcattag
     5461 catagaagtc gcttcgatcg aaggatgttc gatccattgg atacaatttt caggagctcc
     5521 ggcagcgata gcagcatcac gtaccactcg tgctgcttct gcagaagatt tttgggcact
     5581 tggatggaaa gcaaaaacga ttggatttct tgttttcaac gcgatcattg atttgaaaat
     5641 cgttgtggaa gtcggattcg ttgttggagt tactccgcaa accacaccga ctggttcagc
     5701 aatttcaatc aacccttttt ggacatcttc attgatcaca ccaactgttt tatcatgttt
     5761 gatattatgc caaatatatt cggacgcata catatttttg attgctttat cttcgtaaac
     5821 accgcggcct gtttcctcta cagccatctt cgctaatggc atatgttgat ccaaagctgc
     5881 cattgccatt tcatgcacga tatggtctac tttttcttga tcaaagcttt ccatttcttt
     5941 caatgctgca tttgcttttt ctgccaaatc atcaatcatt gcttgtacgt caattgtctt
     6001 cgttttttct ttttctatta ctttagccat cattatcctc ctaatgggtc atggttattt
     6061 gttaaataat tcacatgtat atcatacaat atttattttt ctatgtaaac actttttttg
     6121 tgattttttt cacagttatt tttaaatata aaattgcttt atttacaggt gtttgtatag
     6181 aaaaatatat aaagttattt attattattt atatgacaca cccgattatt caatattgag
     6241 caatatatcc catgctctta tctctttctt ttttgaataa aaaatgaggt aggacatctt
     6301 tctgccctac ctcgtcttta aataagcttt gatacttatt tttcttcctt ctcttctgtc
     6361 tcttccagcg tttctgttgt ttcagttttt acttcaactg tctcagctgg ttcttctgtt
     6421 gtttcaacag tagtcgttgc atcatttgtt acgacagtcc ctggtttgac cgttttgatt
     6481 gctgcacgat cgtactcaag gaagatccct tcgcagtcta aaacaactgt ccgtttgtca
     6541 ttgtcgattt ctgaaacaac tccatgtaat ccgccgatcg ttacgactga atcacctggt
     6601 ttcatgctat ctaacaagtt ttgacgttct tgctgttgtt tcttttgcga acgtgacata
     6661 aaaaaccaca ttgctaatag cgcgataaac atcacaagca ttggtaatgt tcccattttt
     6721 ttcacctcac taattctgta tctacaaaaa acactttagc agaaaaatgc tgataacact
     6781 agttttttac ttaattttta aaagattttc ctttagaagc tttttgcgtt ttctttattg
     6841 aacccgtatt cttcaaaaaa ggcttctcta aattcaagca aattatcatc catgattgct
     6901 tgacgtactt gtttcattag attcaacaag aagtataaat tgtggtaaga agtcaaacga
     6961 atcccaaatg tttcatcaca tttgatcaaa tggcggatat aggcacgtgt atagttttta
     7021 catgtataac aatcacattt ctcatcaatt ggacggaaat catgggcata tttcgcattt
     7081 ttcactacta gacggccttg agaagtcata catgtgccat ttcgagcgat tcgagtaggc
     7141 aagacacagt caaacatgtc tactccacgg ataacaccat cgatcaatga atcagcagca
     7201 ccgacaccca tcaagtaacg tggtttgttt tcggggatca acggtgtagt gaattctaat
     7261 acacgattca tttctgattt aggttccccg acagataacc cgccgattga gtaaccaggg
     7321 aaatccatcc cgactagatc tttcgcgctt tgacgacgaa gatcttcaaa ccctgctcct
     7381 tggatgattc caaataatcc ttgacggtct ggattagcat gagctttcaa tcctcgttct
     7441 gcccatcgag acgtacgttc tactgatttt ttcacgtagt cgtaactttc atcgaatggt
     7501 ggacattcgt caaaactcat catgatatca gagcccaatt tattttggat gttgattgct
     7561 ttttctggtg ataaaaacat acgcgaaccg ttcaagtgat ttttgaaatg cacgccttct
     7621 tcttcgatat tccgcatatc acttaatgaa aaaacttgaa agccgcctga atctgttaaa
     7681 atcggttgat cccagttcat aaatttatgc agtccgccag cttcttctac taagtcttca
     7741 ccagggcgta gccataaatg atatgtatta cttaaaataa cgccagcacc catttctttc
     7801 aactcttctg gggacattgt ttttactgta gctaatgttc ctactggcat gaacattggt
     7861 gtggggaaag ttccgtgtgg cgtgatcaat tcccctaaac gcgcgccagt atgtttttct
     7921 ttcttgatta aacgatagcg aatggcaggt tgcgtcattc atttccctac tttctatata
     7981 aaatatcttg acaactccca ccatcataat cgtttcaagt gctttttgca agttccctcg
     8041 gcttccttct actttattta atattctttt ttttatcctt tttttatgaa taacaaaaaa
     8101 aagaaaaaca ttatgacaga aatatttttt gatacatggt atattggtgt tattaaagaa
     8161 aaggagggaa aaatcatgca atcttctgca gaattaaaac gccaagcaaa agagagtcta
     8221 aaaggacgtt ggggacaagc tgttttacta aatctaatcc ctacattgat tggtattgct
     8281 actattcttc ttattatgat tcctattatc ttgtttgcca ccaccatata tgatggatca
     8341 gcgagcgtct cagaagcgac aacttcagga ggcggtagtt caggaggcgg tggcggaatt
     8401 atttccagca tcattagcgc tttgttcatg agcggtattt cttggactta tcttgatatt
     8461 ttacgtggac aaaaagatca gatcgaacca ttcaaagatg catttcgtgg attcaaaggt
     8521 ttgtttatcg gcggagtgat cagcttagct gtgctgatct cgatctttac aacattatgg
     8581 ggtttgctat tagttattcc tggaatcatt aaaagctatt cttattctca aagctatttt
     8641 atttactacg atatcgtcca agaaacagga caaacaccta aagtactgga tacgatcact
     8701 gcaagtcgaa aattgatgga tggttataaa ggacaacttt tctggttaga tctttcattt
     8761 atcggctggc atatcttagc attagcaact gctggtattg gttatctatg gctcagccca
     8821 tacatttctg caacaaaagc agcattttat gaacaattgc caaaagatat ttaaaagaga
     8881 gtgtgacttg tgaccgaggg ggtggcagga gaacaccgtt tatcgagagg cagtaaaaga
     8941 aacattcagc ccattagcaa aaaggaggtg tgacaacatt catgtcacac ctcctttttg
     9001 tatagttcca gtaaaaagaa aatcaactta gaaacagtta atcattcctc ttcttctgct
     9061 tccggtaatc gatggaactt gatcagttca ttcaccgcga ttggtacgat agataaaatc
     9121 cctaccaaga accagtgatt caaactgatg tgtactaggc cgaatagttc ttgcgtaaat
     9181 ggcagcaaag caacagccgt tgtaatagct aatgccagta acgccatttc taccaaagct
     9241 ttattggtcg ctaaattgac tttgaagatc gattggctgc tgcgtacatt gaacacatgc
     9301 agaatcgatg aataagctaa gatcaagaat gcaacagttt gaccgacatg ctgacttgcg
     9361 tctacccatg ggctgatagt atcgacataa ctacctaaat aataaccaaa caaggttacg
     9421 atggtgaaga cccctgcatt tatggcaatt ttttgcaata aacctcgagc aaagatccct
     9481 tcatttttcg gaatgggtgg ctgatccatg atgccgtgtt ccgctggctc tcttcctaaa
     9541 gcaaatcctg gaagtccatc agcaactaca ttgacaaaca gtagttgcac agctgtaaat
     9601 ggtgctcccc atcctaacag catcgcaatc aaaacaataa agatctccga aatattacag
     9661 cttagcaaaa agttgatagc tttacgaata ttctgataaa ccgaacgtcc ttgactgaca
     9721 gcgtctacga tcgtcgcaaa gttgtcatcc gtcaaaatca tatctgcggc actttgcgcc
     9781 acatctgttc ctgtgatccc catggcacac ccaacgttac ttgctttcaa cgctggtgca
     9841 tcattcacac catccccagt catcgctacg actgcacccg tacgttgcca agatttgacg
     9901 atccggatct tgtcttctgg cgttacccga gcatagacag agagagcttt gacacgcgca
     9961 tctaattctt cgtctgataa ttgatgcagt tctgatcctg aaagtgcttc tgacggatca
    10021 ttcaagatac ctaattcttt cgctatggca ctggcagtca ccacatgatc tccagtgatc
    10081 atgactgttt tgatgccagc ctttttcgct cgcttgattg ctcctttact ttctggacgt
    10141 ggtggatcga tcattccgat cagaccaatc aaacgtaagt ctttttccag tgcttcagaa
    10201 gaaatatctt gcggttcttc gtcataagta gcataaccaa cagcaatcac tctcagtgcc
    10261 cgtttaccaa agcgatcatt aacgatcgcc gcttgttcta catcgcctga actaaagcgc
    10321 ggagcaagaa catcgaatgc tcctttggtg attgagatgt atttttcgcc catttggtga
    10381 actgttgtca tcatttttcg ttcagaatca aaaggaagct cgtttactcg tggatatttt
    10441 tcttctaatt ctgcctttgt atgataattc tcttcaacag cccttacgat ggctacttct
    10501 gttggattac ctttgataac aaggtcgtca tcttcttgct cgaccgtcac atctgtacat
    10561 agtgcagcca ttttcaatat ttccatcgcg ctgttcgtca ttgaatcttc gatattcgtt
    10621 acttcatctt ctttgctcca tacccggcgg acgcgcattt cattttgtgt cagcgttcct
    10681 gttttgtctg agcagataac attagcggtt cctagcgttt caactgctgg caactgtcgg
    10741 atgatcgcat gtttcctcgc catcttctgc acaccgtatg caagtgtcaa cgtaacaata
    10801 acagtcaagg tttccggtac agctgcaaca gccaaggaaa tcgaagtcat gaacatctca
    10861 aggagtggtt ctccttgcag ttcaccaatc acaaatacta aagccgcagc cgctaaagcg
    10921 atcatactga ttcgtttgcc tagctgattc aatcggacct gtaaaggtgt tttttgcata
    10981 gtattttcgt tcaaaagatc cgcgatctta cccatttctg tagccatgcc aatggcagtg
    11041 acgatcgctt tcccacgacc tgcagcaacc gtacatcctt taaataccat attaagttgg
    11101 tcacctaatg actcatcttc ttcagcaata aatgctgcat ctttttcaac tgcttcactt
    11161 tctccagtca acgccgattc ttctacacgt aattgtgatg cttcgatcaa acgggcatcg
    11221 gcagaaatag catcaccgga ctctaaaaca agaatatcac ccactaccag ttgctctgac
    11281 tcaacagtgg tcactttgcc gtctcgtatg acagttgccg tttgtttatt catatcttgc
    11341 aaggattcca gtgctttttc tgcgtttcct tcttggacaa tcgccaatac cgagttgatc
    11401 acaacgatag cgatgatcag cagtccttca aataaatccc catgttctgt agcaaatgcg
    11461 gtataaaagg aaatcaccgc tgctacaaga aggatgatcg ttgtaaaatc ggacaagctt
    11521 cgaaagaatt ttttgatcat cgattctttc ggcgcctcat cgaatttatt caatccctgc
    11581 ttttttcgcc gttcatccac ttcttgtgaa gacaacccag tatctggatc tgcttctgtt
    11641 accttcttta cttcttctat tgtctgcttg tattcttcca tcctattcct cctttaaaat
    11701 cttcgtacaa aaaaaggtac ttattttact ataacatcct ttttttattt acttcaaata
    11761 aagggcttat cgattatttg ttaagaaagc gtaaaattta aagtgcttat atttgcaaat
    11821 gctaccaaag gatgctatta tatagatgta aggaaggagg ataccatcaa atagacagaa
    11881 gaatttctcg atagaacagt gtaagcaaac aaagttgtta gaagtacagg tttcacccag
    11941 acatgcgtgt agagtacaaa cctataaata gtgaatgcgt atggatgttt ggatcacgaa
    12001 gtatccgaaa caagaagtaa tgcgatacgt aacaattgaa taacgtaaac gtctgaaaat
    12061 tgaataggaa caagagatgt ttcaaatgtt gaagatgtaa cgaaaacaaa atttttgttt
    12121 aacatgaaga aattcaacac cgtgctttgc atgtatgatg gaaagttaca atcatttctg
    12181 tttatttgaa tccttccgag gtacaagaaa gccgagctta tcgagagctc ggctttttct
    12241 ttgtcataaa atctttgtaa agcttttttg ttattcaaaa ctagcggctt ttcataacct
    12301 tatttcacga acatcgcatc tccaaaacta aagaaacgat agcgctcttc aatcgcatgc
    12361 tggtaagcag ataaagtcaa ttctcttcct gcaaaggcac taactaacat gactaaagtc
    12421 gattttggca aatggaaatt agttgagaaa gcttctacca ctttgaattc atagccaggc
    12481 gtaatgaaaa tatcagtcca tccgctgtct gctttgattt ctccattaaa tttcgttcca
    12541 atcgtttcta atgttcgaat tgaagttgtt ccgacagcca cgattttccc gccattttta
    12601 cgtacttggt tcaatcgctc agccgcttct tccgtcaaac ggtagaattc actatgcata
    12661 tggtgttctt caattttatc tacgctgaca ggtcggaatg tacctaaccc tacatgtagt
    12721 gtcagataga ctaattcaac accctttgct tcgatctcat caagtaattc tttcgtaaaa
    12781 tgcaatccag ctgttggcgc tgcagcagaa ccattttctt tcgcatagac agtttgatag
    12841 cgatctgggt cttccagacg ttctttgata tatggaggca atggcatttc tcccaatgat
    12901 tcaagaatct ctagaaaaat tccttcatac tcaaagtcaa tgatccgtcc gccgtgttcc
    12961 aattcttctt tcactactgc ttgcaatctg ccatcaccaa aagagattct tgtcccaact
    13021 ttggcacgtt tcgcaggttt gatcaatgtc tcccacgtat ctccttgtgt attcgttaga
    13081 agaaggactt ctaaatgcgc accagtatct ggtttttctc catataaacg agcaggcaga
    13141 actcgtgtat tattcataac tagcgcatca ccaggctgta actcttcaat gatttcatgg
    13201 aaatgtttat cttccatctc tccagttttc cgatccaaaa caagtaaacg agaatgatcg
    13261 cggtctttta atggtgtttg tgcgattaat tcttccggca agtcaaaatc aaaatcttct
    13321 gttgttaaca tatcgtcact cactttctat catgtgcttc atcattctat catgtttttc
    13381 gttctgttgg caaaatcagc actttttcac ttctattttt tgttttccta aataaaaaaa
    13441 ctaccctatt tgtatttttt cattgtttta ggcatactag taatagatag ttcttcattg
    13501 aaaatagaaa aagaggtggc accatgattg aatttcaaaa tgtgtcaaaa atttataaag
    13561 gtggaaaagt cgctgtagag gacgtcaatc tttcatttga aaagggtgag ttcatttgtt
    13621 ttatcggtac gagcggaagc ggaaaaacga cgacgatgcg gatgatcaac cgaatgactg
    13681 atccaacaca agggaaaatt ttgattgatg gaaaatccat ccaagaaatt gatccagtgg
    13741 aattgcgacg tcagatcggc tatgttatcc aaagtattgg attgatgcct cacatgacga
    13801 tccgtgaaaa tatcactctt gtccaaaaat tgttgaaagt cagtcaagaa gaacggaata
    13861 aaacagctga aaaaatgatt gatcttgtgg aattgccccg tgagatgtta gatcgctacc
    13921 cacatgaatt atctggtggt cagcagcagc gtattggagt cgttcgtgcc cttgcagcga
    13981 atcaagatat catcttgatg gacgagccat tcggtgcgct tgatccaatc actcgtgatt
    14041 ccttgcaaga ccttgtcaaa gatttacaag agcgtttggg aaaaacgatc gtatttgtaa
    14101 cccatgacat ggatgaagc
//
LOCUS       DAMEET010000068        13917 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.069, whole genome shotgun sequence.
ACCESSION   DAMEET010000068 DAMEET010000000
VERSION     DAMEET010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.069"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            131..505
                     /locus_tag="PB780_001918"
     CDS             131..505
                     /locus_tag="PB780_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDE0733968.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="PB780_001919"
     CDS             complement(619..1248)
                     /locus_tag="PB780_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDE0733969.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="PB780_001920"
     tRNA            complement(1382..1454)
                     /locus_tag="PB780_001920"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="PB780_001921"
     tRNA            1668..1741
                     /locus_tag="PB780_001921"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="PB780_001922"
     CDS             complement(1879..2043)
                     /locus_tag="PB780_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733970.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="PB780_001923"
     CDS             2338..3666
                     /locus_tag="PB780_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HDE0733971.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="PB780_001924"
     CDS             3868..4701
                     /locus_tag="PB780_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDE0733972.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="PB780_001925"
     CDS             4717..5454
                     /locus_tag="PB780_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HDE0733973.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="PB780_001926"
     CDS             complement(5609..6577)
                     /locus_tag="PB780_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDE0733974.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="PB780_001927"
     CDS             6809..7642
                     /locus_tag="PB780_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDE0733975.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="PB780_001928"
     CDS             7676..8515
                     /locus_tag="PB780_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDE0733976.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="PB780_001929"
     CDS             8541..9101
                     /locus_tag="PB780_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDE0733977.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="PB780_001930"
     CDS             9300..11021
                     /locus_tag="PB780_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HDE0733978.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="PB780_001931"
     CDS             11186..12328
                     /locus_tag="PB780_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDE0733979.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="PB780_001932"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="PB780_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDE0733980.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4752 a         2297 c         2833 g         4035 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggagatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttg
//
LOCUS       DAMEET010000069        13898 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.070, whole genome shotgun sequence.
ACCESSION   DAMEET010000069 DAMEET010000000
VERSION     DAMEET010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.070"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            157..2322
                     /gene="acm"
                     /locus_tag="PB780_001933"
     CDS             157..2322
                     /gene="acm"
                     /locus_tag="PB780_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HDE0733981.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2594..3079)
                     /locus_tag="PB780_001934"
     CDS             complement(2594..3079)
                     /locus_tag="PB780_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDE0733982.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3169..3514
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3559..4200
                     /locus_tag="PB780_001935"
     CDS             3559..4200
                     /locus_tag="PB780_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDE0733983.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4219..4854
                     /locus_tag="PB780_001936"
     CDS             4219..4854
                     /locus_tag="PB780_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDE0733984.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4872..5687
                     /locus_tag="PB780_001937"
     CDS             4872..5687
                     /locus_tag="PB780_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDE0733985.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            6006..7442
                     /locus_tag="PB780_001938"
     CDS             6006..7442
                     /locus_tag="PB780_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDE0733986.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7631..10078
                     /locus_tag="PB780_001939"
     CDS             7631..10078
                     /locus_tag="PB780_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDE0733987.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10308..11375
                     /gene="rlmN"
                     /locus_tag="PB780_001940"
     CDS             10308..11375
                     /gene="rlmN"
                     /locus_tag="PB780_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDE0733988.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11568..12671)
                     /locus_tag="PB780_001941"
     CDS             complement(11568..12671)
                     /locus_tag="PB780_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDE0733989.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13224..13772
                     /gene="aac(6')"
                     /locus_tag="PB780_001942"
     CDS             13224..13772
                     /gene="aac(6')"
                     /locus_tag="PB780_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HDE0733990.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         4758 a         2301 c         2942 g         3897 t
ORIGIN      
        1 ttttttggca tatttatttt ttataaatat ttgtaaaata actatcaatt ttttagttaa
       61 aagattggaa atgagtgggg gaggaattga agaaaggatt tttgagagat gatatagtag
      121 gttttatttt tagttaaaat aggggagata gaaaaaatga aaaagtgtag aaatattatt
      181 ttatcaatgt tatttattat tactaatata acatcactga ttcctgttca tgtctatgcg
      241 gatgcaggca gagatatcag cagtaatgtc acttcgttga ccgtggaccc aacgaatatt
      301 acagatggag gaaacataaa agtaaaattc tcgtttgacg aaaaaaaaca aaatatccaa
      361 ccaggtgatt atctttggat caattggcca agtgaaggaa atattcgcgg tgaaggattc
      421 caaaaagaaa tcccattaat gattgaaaat aaaaatgttg gaactttaac ggtcagaaaa
      481 gattctgcgc aggttgtgtt taatgaaaat attaaaaacc tagactcagt agaaggttgg
      541 gggcaatttg agatacaggc cagaaacgta accgataccg gtgaagaaaa tacgggacca
      601 ttcaccgtga cgagcggtga taaaacagct actgtaaatg tgacaaagcc agcttctggt
      661 tcttcttcta gcgtttttta ttataaaaca ggagacatgc tacctgaaga tactaagcac
      721 attcgatggt ttttgaatat taacaataac ggcacttatg ttgaacaacc agttaaaatc
      781 agtgatgaaa tccaaagtgg acaaagatta gatccaagta cgtttgaaat taatcaaata
      841 cacttaggtg aacagaaggt ttatagagga gaagagggaa tccaacaatt tttacaagat
      901 ttccccagcg ctacatttaa tttttcagta actgataatt acattgaaat aactatacca
      961 aaaaactttg tcaatcttag gaaaataatg gtgagttata agacgatcat agaaaaccca
     1021 gaacaaataa attttgagaa tcattcggaa gcttggttta aagaatttaa taaacctgct
     1081 gttgatggag aaagtttcaa tcatacagtt aaaaatattt cagcttctgg tggagtcaac
     1141 ggtacagtta gaggcgaatt gaagattttc aaatatatca atgacacaga gattggtatc
     1201 ccaaatgtga ctttcgaatt aagacgagca gatgaacagc ctattcaagg acagtctagt
     1261 atcctgttga cttcgaatga acagggagaa gctagtatca aaggcctgca agtaggagac
     1321 tatgttgtaa aagaaaaaga agcacccaat tggattgatt ttgatccact aagttcaaat
     1381 gaactcaaat tctccataaa cgaaaatgat acagagggag tcagtttacc tatttataac
     1441 aagaaaaagg ttacgaacat cacagcaact aaaatttgga acggtggaac cactccaaga
     1501 ccgagtattt attttaaatt attcagagca agtacaaaca attgggaacc agttcctgat
     1561 gcagaaacaa aacgattaga caatggaata acttcagtga cttgggaaga tatccaacag
     1621 tatgatgata gtggaaacga atatacgttt aaagtgcaag aagtagatca aaacggaaat
     1681 gattatgtcc cttcaggtta tcaaaaaatt gaaaacgggc tagtcgttac aaatgagaat
     1741 aaagaagtca tttcagtctc aggacaaaaa acatgggaag acaatgagaa ccaagacggc
     1801 aaacgaccga cgacgatcac agtaaatcta ttagccgacg gtcaaccgat ccaacacaaa
     1861 gaagtaagtg aaaaagatga ttggcgctat cgtttcacaa atctgccaaa atacagagac
     1921 gggcaagaaa tcatctatac tgtaacggaa gacaacgtgc ccgaatacag tacgacgatt
     1981 gacgggtata acatcaaaaa cagttataca ccggaaaaaa caaatatatc tataccaggt
     2041 agccatataa caccgtggaa aaattttcca gagaagacag agagagagaa aattagaaat
     2101 ctattctctc aaaaattgca aatatttgct catacaggag aatccagcga acaaagaagc
     2161 aattatatta gtaagagagc tgtacctaaa cagataacaa ataaatcaaa aagcattctt
     2221 cctaaaacaa gtagtaaaaa atctagtttt gctgtaatca ttggtttatt gatagtaact
     2281 atctgtgctg ggatattctt acaaaaatat aaaaaagttt agttagaata taatattttt
     2341 tgttaaatat gactgaaatc aaaagaataa aattccgatt tataatcgaa aaaagaagca
     2401 tttttatata tgcttctttt ttcgtagaat caataaatat ggatttggct aactatggaa
     2461 gaatactcgt attagttttc agcgttcata ctagttgtta cattgtttga ttggccaaaa
     2521 gaatttctga tatattttgt gctaaataaa gaaaaagacg cttgaattgg gaaataagaa
     2581 aactactcta tcattacaaa ttttcataaa ctggcagatg ttctaagtaa acaagtatct
     2641 tttcaaagtc ggaaaaatcc ataatcaacg tagtggtatt gatatttgga tgaaagccta
     2701 cggtatcttg acgatcaata gcagcatcga tcacgacttg gatttttttc tctttgtcgt
     2761 gcatcaatgc taatggagtc actgtacctg ctggaacgcc taggaggtca ggaagctgtt
     2821 cttcagaaac aaaagataag cgcttttctt ttaattgttc agctagttgt tttaaatcgg
     2881 cgtgcttttc atcaggcaag atgacaagat agatttgttt tccttttctt gatttcaata
     2941 atagattttt tacttgcgga ccaggtaggg aaaaagggac atttttgact gacgtaatcg
     3001 caggatgatc gactttttga aaagagatac ccagtttttt tagtagttca taagcttctt
     3061 gttcggttgc acgactcatc gcgcatcacc ctcctttttt tattagtgta tcataaaaaa
     3121 gagttgacaa atgaaaaata atcatgtaaa ttacacgtaa agttaaaaca aatgacatcg
     3181 aaaagaagag tagccgttag gtgtttttag agagtctctg gttggtggaa agaggcagca
     3241 gttaaccgtg aaacacatct tggagtcgat tttctgaagc gtatagtagg aaaatccggg
     3301 gagcccgtta cagctgcggt cgttgctaga tgaccagata aggctggctt ttccgtgagg
     3361 agtcggtaaa aaaaggtgga accacgtgat taacgtcctt tgatcttcgg atcaaaggac
     3421 tttttgtttt catctgaacg gcaacgactg tgtgaggttt tttctgcaag gaaaaaataa
     3481 acagaggtgg aaccacgtat aatacgtcct cttggcattt gccaagagga tttttttatt
     3541 ttaaaggagg aaaagaagat ggagtatttg attgaagtta tgccgcaatt attagtagga
     3601 gcactcacaa cattgaaatt atttttattt accttgattg gctcattacc attagggatt
     3661 gttttagcat taggattgtc tttgcatttt aagccgctac gctacttgct tcaaacatat
     3721 gtatgggtta tgcggggaac accgctgttg ttgcaactga tttttatttt ttatgggttg
     3781 ccgacgattg ggatcgtttt tgatcgtttc gaagcagcac tcattgcctt tatcttaaac
     3841 tatgcagctt attttgcaga gatctttcgt ggagggatac aatcgattcc tcaaggtcaa
     3901 tatgaagcag cgcaagtttt gcaattaagt ccctttcaaa cggtgacgaa aatcgtcatc
     3961 ccacaagtga caaagattgt tttaccttct gtagggaatg aagttatcaa cttggtcaaa
     4021 gattcttcct tgatctatat attaggttta ggtgacgtaa tgcgtgctgg gaaaatcgcg
     4081 atggaacgcg acgcgacttt actgcctttg ctaggcgtgg caatcatcta tcttacattg
     4141 actggtattc taacaatcgt aatgaaacaa gttgaaggaa aattaaatta ttaccgctaa
     4201 ctgataggag gaagaaatat gattgaattg aagaaagtga acaaagcatt tggtgaaaaa
     4261 aaaataatca aagatttgaa tctaatcatt ccagatggga aaacattagc tatcgtaggt
     4321 ccttccggtg gcggtaaaac cacgctttta cgtatcttgg ctggcttaga aagttctgac
     4381 agcggagaat tttatttaga cggtgtaccg ttcaacccat tcgagcttaa aatgaaagaa
     4441 caagttgtag gagtcgtatt ccaagacttc cagctatttc cacatctgaa tatttttgaa
     4501 aacattacct tagcaccaaa aatggtattg aaacagacaa aagaagaata caccaaaaaa
     4561 gcagaagaat tagcggcttc attagggatt agtgatttat tgaatatcta tccttatcag
     4621 ctttctggcg gacaaaaaca gcgggtagcg attgccagag cattggctat gaatccacgg
     4681 gtattggctt atgatgaacc aacaagtgct ttagatcctg aattacgcca gcaagtcgag
     4741 cgtttgatcc tatcattgaa agaagatggg gtaacccaaa ttattgtcac ccatgattta
     4801 gtatttgcag aaaatgtcgg tgatcaatta ctaaaagttt ccccaattca ttaagaaagg
     4861 agaagataga gatgaaaaaa accagttttc tattcgcttt gatcgcaggt cttcttcttt
     4921 ttgcaggctg ttcgaacaaa aaaacttcag cagataattg ggcgactatc aacgaagaga
     4981 aaaaaatcgt tataggttta gacgatacat ttgttcctat gggatttaga gataaagacg
     5041 ggaagctcac aggatttgat attgatttag ccaaagcagt ctttgaacaa tatgggatta
     5101 cggtagactt tcagcccatt gactggtcaa tgaaggaatt tgaactgaac aatggaacga
     5161 tcgatttgat atggaacggc tattccaaaa cgccggcaag agaaaagaaa gtgcaattta
     5221 caaaacctta tatggaaaac gatcaagtgc tgatcacacc taaagaatca ggaatcacaa
     5281 actttggaca aatgaataat aagagattag gtgcgcaaaa tgggtcatct ggttatgatg
     5341 tatttacgaa acaaccagaa gtattaaaag agatagtcaa aaaccaagat gctgttttgt
     5401 atgatagttt taatgaagca ttgatcgatc tgaaaagcag acgggtcgat ggtctgctga
     5461 tggataaagt atacgcagac tattatttga aacaaagaaa cgaactagaa gacttcaaca
     5521 taacaaaagg ttcttatgag agtgaggatt ttgcagttgg tgcacgcaag tcagatagta
     5581 cattagtgaa aaaaatcaat gaagccctag atcaactaga gaaaacaggt gaattccaaa
     5641 aaatatccga taaatggttt ggcgaagatg ttacgccgag aaattaacaa ataggggact
     5701 gtgacaaaag acgtgtcaca gtcccttttt ccgaataaac agtgttctcc tgccaactcc
     5761 ttgtgactcg gagctgaatg gcaggctcac aacctcttgg taaacggtgt tcaaaagcca
     5821 gtttttcggt attaagccaa atttatgcaa aaatgtagaa agccattttc acaaaatttt
     5881 tcttactact cgaaactaag cgactttttc acaacctcct ttacatattt ttattttgtt
     5941 gtggaagggg tttcgtgtta ttctaaaaga ggaacgattg aaggttatag gaaaggaaga
     6001 gatgaatgga acacaaagaa cttacctcat ttccaaaaga ttttctgtgg ggctctgctt
     6061 ctgcagcgta tcaagtagaa ggagcttggc aagaagatgg gaaaggagaa tcagtctggg
     6121 atcgatttgt acggatacct ggaaagacat ttaaaggaac gaacggtgat ttggcagtag
     6181 accactatca tcgttataaa gaagatattg ctttaatgaa agaacaaggt ctgaaagctt
     6241 accgcttctc tgtagcttgg acacggattt ttcctaatgg acgaggagaa gtcaatcaag
     6301 ctgggctcgc tttttatgag agattgatcg acgaattgat tgaaaacgat attgaaccta
     6361 tgttgacttt atatcattgg gatcttccac aagcattgca agatgaatat aatggttggg
     6421 aatcacgtca gatcattgaa gatttcactc attatgcaga aacattattt gaggcattcc
     6481 gcgggaaagt ccattactgg attagtttga atgaacaaaa tatcttcact tcattaggtt
     6541 atttgttagc agcacatccg ccaggtgtga cagacccgaa acgaatgtat gaagttaacc
     6601 atattgctaa tttagcaaat gcatctgtca tcaataaatt tcacgagtta gaaatgcctg
     6661 gaaaaatcgg accaagcttt gcgtatacgc caaattatcc aattgacagt aatcctgaaa
     6721 atgttctggc tgctgaaaat gcagaagacc tgatggccaa ttattggatg gatgtctata
     6781 tgtggggaaa atatccgatt gctgccatga ggttcttaga agaaaaagga tgggcaccaa
     6841 cgattgaagc tggcgatgca gaattgctag aatctgctaa accagatttt cttgggatca
     6901 attactatca aacggcgacg aatgcattca atccattaaa cggagttggt gctggaaaaa
     6961 tgaatacgac aggaaaaaaa ggttcatcag aagaaactgg agttcctgga atgtataaaa
     7021 aagttgagaa tccatttgtc gaacgaacaa attgggactg ggagattgac ccagaaggat
     7081 taaggatcgg actgcgtcgt atcacaagcc gttaccgcat gccggtactg attacggaaa
     7141 atggcttagg agagtatgac aagttgacag aagataaaca aatccatgac gactatcgaa
     7201 tcaattactt acaaagccac gtcaaagcca tcaaagaagc aatttcggat ggagcagaag
     7261 tcctaggcta ttgcacctgg tcttatacag atttgcttag ctggctgaac ggttatcaaa
     7321 agcgatatgg atttgtatat gttgatcaag acgaaacaca agaaggatct ttggaacgat
     7381 ataagaaaga cagttattac tggtaccaaa aactgattga agaaaatgga gagaaccttt
     7441 agttcacata cttaaaacaa gaaggtacgg ctggatatgg ccgtgccttt tcttgataaa
     7501 atcatttaga aattaagaaa aactgtaaaa taagctgaaa aatgaaagaa agcgtcagtt
     7561 ctttcaaaca aaactttagt tcccttactt ttttttgtat aatgaaactt gatgaaatga
     7621 gggataataa atggcacaaa agaaacggcc tactaaaaaa aggaaaaaga cgaaaaaaac
     7681 gaaaaagcaa aaacagcagc aggaacaaat gctgacgatt ggtgttgggt ttgccttgat
     7741 tttatttgct gtcttcggct ttttaaaatt aggtttttta ggaatattga ttgcaaacgg
     7801 ctttcggatc attgcaggaa atacttacca aatcctctgt cttttattag ccgtattagg
     7861 attttggatt gtcattaaga atacagagtt ttcaattggt aaaaaccgac gctggtttgg
     7921 cggcatactc ttttatttcg gtatcttatt attgttacat gcccatttat ttggcaaact
     7981 ccatactggg gaaccaaata tcatggggac gacatgggat ttgttagcca gtgatataaa
     8041 acaaagtcaa gtagacaata acgttggtgg tgggatgatt ggagcaatcc tttatcattt
     8101 tacttacttc ttgattgcac agcctggaag ttatttagca gcagtgctat tactggcagg
     8161 aggagcattc ctgttcagta atctggaagg ctatcagctg ttaaatggta tccaatcaat
     8221 tggagaacga gtacaggaat tattggaagg tgatcctgcc aagcaagctc gaaagcaagc
     8281 ggcaaaggaa gagagaatga aacagcgcgc agaagcgaaa gaagcaaggc gcttagctgc
     8341 aaaagaagct gcagaaaaag aagctgcaga atatgagaag aaaaaagcgg tgagccagca
     8401 aagggaaaat actgctgatg aatggcagga accagaaaat acagagccgg agcagctaag
     8461 ttttgttcca atcgacagtt ttcaagaaaa tatccatcct gcgaatctag agaaaccagt
     8521 accagataca ccaaaacaga ccaatacagc agaaggtttt tcagatgagt tgccagagga
     8581 tgacggtacc tcattagaat ttgagattga agcagaacag gaaaatcaag actatgaatt
     8641 accgacagtt gatttgcttg attctatacc aactgtggat caaagtgatg agtataaaaa
     8701 aattgaaaaa aatatcggcg tattagaaca aactttccaa agtttcggtg tcgatgccaa
     8761 ggtcgtcaaa gccagcttag gaccatccgt cacaaaattc gaagtccagc cggctgtagg
     8821 ggtaaaagta agtaaaattg tgaacttaac agatgatatc gcgcttgctt tagcggcgaa
     8881 agatgtccgg atggaagcac cgattccagg taaatcactg attggtattg aagtaccaaa
     8941 tggaaagatc agtatggttt cctttagaga aatcatcgaa gcacaaccaa accacccaga
     9001 taaattacta gaagtacctt taggacgtga tgtttctgga cgggtacaaa cggctgatct
     9061 ttcgaagatg cctcacttac tcgtggctgg ttcgactggt agtgggaagt cagtagcaat
     9121 taatgggatt atcacaagta tattaatgcg tgctaagccc catgaagtaa aactgatgat
     9181 gatcgatccg aaaatggttg aattgaatat gtacaacggc atacctcatc tgttgacgcc
     9241 ggtcgtgacg aaccctagaa aagcagcgca agccttacaa aaagtagtac aagaaatgga
     9301 agaacgatat gaaaaattcg cggcaaccgg agtaagaaat atttctggtt acaatgaatt
     9361 tgttcaacag aaaaacttgg aaaacggaac gaaacatcca accttaccat tcatcgtagt
     9421 gatcgtagat gaattagctg atttgatgat ggtagccagc aatgaagttg aagatgcgat
     9481 tattcgttta gcgcaaatgg ctcgggcagc ggggattcat atgatcctag ctactcaacg
     9541 tccaagtgtg gatgttatca caggaatcat caaagcaaat gtaccttcca gaatggcatt
     9601 tgcggtatca agcggtacag attctcggac gatcattgat tctaatgggg cagaaaagct
     9661 cttagggcga ggagatatgc tattcttgcc aatgggtgag aataaaccga tccgtgtgca
     9721 aggagcattc atttctgacc acgaagtaga acgggtcgtt caatttgtta cagatcagca
     9781 agaagctcat tatgaagaga aaatgatgcc gacagatgaa gttgaaacga caggtgctcc
     9841 agaacagcca caagatgagc tgtttgaaga agcaaaagca cttgtcgttg aaatgcaaac
     9901 ggcaagtatt tcccttctcc aaagaagatt tcggatcggt tacaaccgag cagcacgttt
     9961 agtagatgaa ctggaagctc atggagttgt tggcccgtca gaaggaagca agcctcggaa
    10021 agtattcatt gagcaggaag aaacatcaga aacgttgccg gacgataatg acatgtaaaa
    10081 cttgtgtgat gaagggttga aaaacgaata accaataaaa agacaaggca tgatgatacc
    10141 aaaagaaaaa atggtttttt tccatctttt ctaatacggt atgatgtctt gtctttttag
    10201 tttttgtgag attaaagttc tattcgattt atttgtgaag tattgcatta aggaaatgaa
    10261 tcatgtatag tatgtagcga aactttgaga aaaagaggga aaatactttg gaaaaacagt
    10321 ccatttatgg cttgacgaat gaagaattaa tcaattggtt tatagaaaac ggcgagaaaa
    10381 aattccgagc tgcccaagtt tgggaatggt tgtatcaaaa acgtgtaagt aatttcacag
    10441 aaatgacgaa tctatcgaaa caattgatcg aaaaattatc ggagcatttt atcatcaatc
    10501 ctctaaaaca gatggtcgtc caagaagctt cggatggtac agtaaaatat ttgtttgaac
    10561 tccctgataa aaacatgatt gagactgtac tgatgagaca agaatatggc ttgtcagttt
    10621 gtgtgacgac acaagtagga tgtaatatcg gctgtacgtt ttgtgcaagc gggctgctca
    10681 aaaagaaccg tgatctgact gctggagaaa ttgttgcaca gatcatgatg gtccaacatt
    10741 attttgatga gcgcaatctt ggcgaacgcg tttcacatgt cgttgtaatg gggatcggtg
    10801 agccatttga taattacgac aatgtaatgg actttttgca tatcatcaat gatgccaaag
    10861 gattggcaat tggtgctcgg cacattacag tttccactag tggattagca cacaaaatca
    10921 aggaattcgc taataacggc ttgcaagtca atctggctat ttccttgcac gcgccgaata
    10981 atgaggtgcg aacatcgatc atgcggatca accgaagttt cccaattgag aaattgatgg
    11041 aagctgtgga tgagtattta gagaaaacaa accgtcgaat cacgtttgaa tatatcatgt
    11101 tgaatcaagt aaatgataga ccagagcatg cgcaacaatt agcagatcta ttaaaagata
    11161 agaaaaaatt agcatatgtc aacttgattc catataatcc agtcagtgaa catgatcaat
    11221 attctcgcag tccaaaagct gatgtattga gattttatga tgtattgaag aaaaacggcg
    11281 taaactgtgt gatacgtaaa gaacatggta cagatattga tgcagcttgc ggacaattgc
    11341 gaagcaagca aatgaaaaaa acagttgcca aataatcgta tcctactcgt tttttcaatt
    11401 accataaaag gagcagtttt cttaattaag gaactgttca acttaaataa gagaaaagat
    11461 tctatagcga aagctataaa gctttttttg acaagaaaaa ccctcatcaa gcaaagaaat
    11521 gtcctcaatt tgatgacagc ctgcttgatg ggggttttag caattattta actgattttc
    11581 ttatatcctt gctggctttc ttgtgtagca tgataagttt ctacgtcaag aatggcctgt
    11641 ttaaattttt ctcgggtgat ttcataaggc acatgtttaa tatcatttgt tgccatggct
    11701 ttatctaaga ttgcttctaa atcggtcgtg acattgagat tcatgtcggt taacgatact
    11761 ggaagttggt tctttttcat aaacacatag attttatcaa acaaatcgaa ctgctgatcc
    11821 atcatcaata aggtcagtac accataactg acagatgctc cgtgaaggta tccctccatt
    11881 ttttctagaa cagtcgtacc gtaatagaat gagtgtgcca tagcagaatt atagttattg
    11941 ttaactaaaa cagaaaccaa tccggtagta acaatgatat ccgaaacgac ttgttcaatt
    12001 gcatatgagg gacgatcgtc ggcactatct ttcaatgctt gcgtaccata agtcaagaga
    12061 cgatcaatgc acatttttcc gatttggatt cctaatctat ccgtgtagcc tagatcatcg
    12121 ccacgagcag aaaaagtaga ttcgacttct ttggataaag catcgccaat acctgcccat
    12181 aagtatttat caggtgcttc ggcaataatt gctgtatcaa tgaaggtgtg tacaggtggt
    12241 tttgaaggaa agaagagtcc gtccattacg ccttcttcgt tatagatgac acataccgca
    12301 gtgacgggcg aacagttcga accaatagtg ggaaaggtga agactggttt agctagtttt
    12361 tcaccaatga cttttacagt atcgcaagca cggccaccac cgactgcaaa caggacgtct
    12421 gctttttgga cactttcatg ctggatcaac tgttcaatat ttccataagt tgaattaccc
    12481 ccgtaccaga gactggcgac aacctctatt tcctgtgtca aagctacttt tattttgttt
    12541 tctgcctttg aaagtgcagt cttccctcca atcagcacta cttttgtccc atattctttg
    12601 acgattccat ttgctttttc atacgcatct gttccaatgg tataatttgg taagaaaaca
    12661 ctttgactca tattcatttc cttctttctt cgttgaaacg ggttaaaatt ttaaagagtg
    12721 atcgatctga ctgcggctgt agatggagta gaggtgaacc taagacagga taagtgatga
    12781 ataaggtggc atgaacacct tgaaatcatc aataagtatc ctaaaaacga acaagtacaa
    12841 aaacattgaa aacgacaata atgaattttt gttcctctta gtatttctac cgattccctt
    12901 tgcttttttg taattatcta aaaattctaa aaattaagat tattatatga aaacaggttt
    12961 tatttgtcaa ttcataattg gtttttttta aagcaaaaga taatctatat catgatgaac
    13021 aaacagagtt ttttcctcca attgatggct ttcctgatct ttcctatttt gaataaagtg
    13081 tttctgtata atttttttat tttataagta agataaataa tgaaagaata gagatactga
    13141 ttggtagcta aagatttttg agcgagaaca tttcgttttt tcaaaaggac tgttagaata
    13201 agcaagaaca agggggaaga cgtatgataa tcagtgaatt tgaccgtaat aatccagtat
    13261 tgaaagatca gctttctgat ttactgagac tgacttggcc ggaagaatat ggagacagct
    13321 cggcagaaga agtagaagaa atgatgaatc cagaacgaat cgcggtagca gcggtagacc
    13381 aagatgagtt agtaggattt attggtgcaa tccctcaata cggtatcaca ggttgggaat
    13441 tgcatccatt agttgtagaa agctcccgac gaaagaacca aataggtact cgattagtca
    13501 attacttaga aaaagaagta gcttccagag gaggaatcac gatttattta ggtacggatg
    13561 atttagacca tggaacaacg ttaagtcaaa cggacctgta tgagcataca tttgataaag
    13621 tggcttccat ccagaacctt cgtgaacatc cgtatgaatt ctatgaaaaa ttaggttata
    13681 aaatcgtagg tgtcttacca aatgcaaatg gctgggacaa accggatatt tggatggcaa
    13741 aaacgattat tcctcgacca aattctcaat aataaaaaga ggttgtgaca gaagtaatca
    13801 gcttcaagaa ataagaagga atttacgaaa attacttctt gaatttttgt gaaattccaa
    13861 cttatttccg aagaagctac ttttggaaca ccgtgtac
//
LOCUS       DAMEET010000070        13816 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.071, whole genome shotgun sequence.
ACCESSION   DAMEET010000070 DAMEET010000000
VERSION     DAMEET010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.071"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="PB780_001943"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="PB780_001943"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDE0733991.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="PB780_001944"
     CDS             complement(2260..2511)
                     /locus_tag="PB780_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HDE0733992.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="PB780_001945"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="PB780_001945"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDE0733993.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="PB780_001946"
     CDS             3461..3679
                     /locus_tag="PB780_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0733994.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="PB780_001947"
     CDS             complement(3968..4480)
                     /locus_tag="PB780_001947"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDE0733995.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="PB780_001948"
     CDS             complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="PB780_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDE0733996.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6933..7376)
                     /locus_tag="PB780_001949"
     CDS             complement(6933..7376)
                     /locus_tag="PB780_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HDE0733997.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7377..8165)
                     /locus_tag="PB780_001950"
     CDS             complement(7377..8165)
                     /locus_tag="PB780_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDE0733998.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8162..9103)
                     /locus_tag="PB780_001951"
     CDS             complement(8162..9103)
                     /locus_tag="PB780_001951"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDE0733999.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQKVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9280..9867)
                     /locus_tag="PB780_001952"
     CDS             complement(9280..9867)
                     /locus_tag="PB780_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HDE0734000.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10088..11395)
                     /gene="obgE"
                     /locus_tag="PB780_001953"
     CDS             complement(10088..11395)
                     /gene="obgE"
                     /locus_tag="PB780_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDE0734001.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11698..12708
                     /gene="gap"
                     /locus_tag="PB780_001954"
     CDS             11698..12708
                     /gene="gap"
                     /locus_tag="PB780_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDE0734002.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12793..13728)
                     /locus_tag="PB780_001955"
     CDS             complement(12793..13728)
                     /locus_tag="PB780_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDE0734003.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3810 a         3094 c         2436 g         4476 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc
     4681 ataaaaatta ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga
     4741 cgtatatcaa ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt
     4801 gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg
     4861 aatgaataag accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca
     4921 actagagagt ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa
     4981 acaatcgcag cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct
     5041 aaataaaggc taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca
     5101 aatacttgca gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg
     5161 ttccattcat tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct
     5221 tcttgagtac caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca
     5281 aatttcagat gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga
     5341 aataaaaact gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca
     5401 attcgctcta ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct
     5461 ctcatcaagt caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg
     5521 acattttcac ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat
     5581 aaatctcgca tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat
     5641 cctttagctg atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc
     5701 attattttcc cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga
     5761 tggatatgat tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt
     5821 gcttttcttt cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca
     5881 tcaaacgtcg caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt
     5941 agtctgggag caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg
     6001 ctttcttcaa atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct
     6061 aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc
     6121 gctactaaat ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat
     6181 gctactccga ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga
     6241 acaatcgcat aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg
     6301 tctacaccag cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca
     6361 gtgatgatca actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca
     6421 tacccatgct caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt
     6481 gtttctttca ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg
     6541 attttttctc cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt
     6601 atcagatacg gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt
     6661 tcctcatggt aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca
     6721 cggatcattt ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt
     6781 gcttgtctca ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa
     6841 agcagaaaga aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga
     6901 tcttttgtac gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg
     6961 ggcgattgag ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct
     7021 tgtaactccg cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc
     7081 tgttcttctt ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc
     7141 tgagtcagct gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat
     7201 acaatcagtg caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag
     7261 gaagtgaagc caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata
     7321 atcaaaacta aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac
     7381 tttttgttaa aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat
     7441 tttgatgcaa cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg
     7501 cggacgatca tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa
     7561 tagcttccgc ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact
     7621 gtcgtaacag ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg
     7681 actgcaaatt tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata
     7741 gaggcgatat taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt
     7801 gtcaaaacca tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg
     7861 aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc
     7921 ccgacttctt ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct
     7981 accttgaaag cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct
     8041 acaaatgcca ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa
     8101 cagatttgtg cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa
     8161 ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg
     8221 tcgtgttttc aaacactttt tgtgcttctt tttcaagctc taaagctgct tttcctaaat
     8281 atctcgcact gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag
     8341 cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa
     8401 atgtgctttc atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt
     8461 ttctcgtatc gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt
     8521 ttccattgat cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt
     8581 acagtggtcc agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat
     8641 gatcatgttc aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat
     8701 aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca
     8761 gcggataact taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc
     8821 cttttggccc gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga
     8881 gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt
     8941 ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca
     9001 accaaacttc attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt
     9061 ttgcaggaac acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct
     9121 ttaagttcct ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg
     9181 ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa
     9241 acagcagcga acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact
     9301 ttcattgtcg ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt
     9361 cgcatgattt tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa
     9421 gcgagtaatt tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc
     9481 ttcatcagca atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc
     9541 gaacacggtc tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc
     9601 ggcaaaccat cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat
     9661 taatccaccc gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa
     9721 ttgatgtgtc ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg
     9781 aacagcatgt aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa
     9841 atttaaaatg attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt
     9901 attttacctt taattgtaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg
     9961 gctgtgacaa aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga
    10021 tcacttctgt cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt
    10081 tgcttgccta ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt
    10141 tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac
    10201 gcatcactgt ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac
    10261 cagataagat ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat
    10321 tgaatccata gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg
    10381 ttacttctaa caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc
    10441 cagaaattgg aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct
    10501 gttctttgaa tttcactaga ttttccggtg catccggcat atccattttg tttgccacga
    10561 tgatttgcgg acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa
    10621 gatagtcttc ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta
    10681 ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc
    10741 cttcaatcaa gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca
    10801 ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg
    10861 aaataacgga tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg
    10921 ccaatacttt caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa
    10981 tttccggtgc aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac
    11041 cttttgccac aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct
    11101 ccgcatctcg gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac
    11161 cgtgcatccc tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc
    11221 gaaaatccat caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac
    11281 caccgtctcc gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca
    11341 ttccgtctcc gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca
    11401 taattaagtc ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca
    11461 aaagtcttac ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt
    11521 gtttaccttc ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag
    11581 tataattttt tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa
    11641 ttgtattaat tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg
    11701 acagtaaaag taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc
    11761 aaagaagtct cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg
    11821 ttagcacaat tattgcaatt tgactctact catggtactt atcctggcac agttactgca
    11881 acagaagact caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca
    11941 agcaaaatcc cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt
    12001 tatacatctg aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt
    12061 tctgccccag ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat
    12121 gaaaacgaca aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct
    12181 tatttcttaa ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac
    12241 acttccacac aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt
    12301 tcagctgctg acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt
    12361 atccctgagc tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt
    12421 tcattaactg aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa
    12481 gcaatcaaaa aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt
    12541 tctagcgatg tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta
    12601 acaactgctg gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga
    12661 tttacttgcc aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca
    12721 atcgataaaa gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac
    12781 ttttaaactc gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt
    12841 ttcaggatct gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt
    12901 tggtcccttc gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc
    12961 gtctccatgt ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc
    13021 ttcaccgaaa aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg
    13081 aaatgctgga agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga
    13141 ataaccgcaa tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat
    13201 cataactgtt gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt
    13261 gttttctagc actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc
    13321 aatgtaatcc ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc
    13381 catatcatga aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg
    13441 agccacaaga gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt
    13501 cagaccgtct tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt
    13561 atgtgcatcc gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc
    13621 tttctcagaa ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac
    13681 aatcagagct aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc
    13741 tcatttctct tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa
    13801 tgttcatttt tataac
//
LOCUS       DAMEET010000071        13786 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.072, whole genome shotgun sequence.
ACCESSION   DAMEET010000071 DAMEET010000000
VERSION     DAMEET010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.072"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(92..1459)
                     /gene="nhaC"
                     /locus_tag="PB780_001956"
     CDS             complement(92..1459)
                     /gene="nhaC"
                     /locus_tag="PB780_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDE0734004.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1568..2989)
                     /locus_tag="PB780_001957"
     CDS             complement(1568..2989)
                     /locus_tag="PB780_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDE0734005.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3232..5109)
                     /gene="tdc"
                     /locus_tag="PB780_001958"
     CDS             complement(3232..5109)
                     /gene="tdc"
                     /locus_tag="PB780_001958"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HDE0734006.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5400..6656)
                     /locus_tag="PB780_001959"
     CDS             complement(5400..6656)
                     /locus_tag="PB780_001959"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDE0734007.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(6768..6973)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7236..8537
                     /locus_tag="PB780_001960"
     CDS             7236..8537
                     /locus_tag="PB780_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDE0734008.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8606..9205)
                     /locus_tag="PB780_001961"
     CDS             complement(8606..9205)
                     /locus_tag="PB780_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDE0734009.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            9459..10340
                     /locus_tag="PB780_001962"
     CDS             9459..10340
                     /locus_tag="PB780_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDE0734010.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            10353..11963
                     /locus_tag="PB780_001963"
     CDS             10353..11963
                     /locus_tag="PB780_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HDE0734011.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(12040..12699)
                     /locus_tag="PB780_001964"
     CDS             complement(12040..12699)
                     /locus_tag="PB780_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDE0734012.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(12982..13647)
                     /locus_tag="PB780_001965"
     CDS             complement(12982..13647)
                     /locus_tag="PB780_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HDE0734013.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
BASE COUNT         4030 a         2786 c         2528 g         4442 t
ORIGIN      
        1 aaattcacaa aaatttaaaa agcaattttc ttgagtttct tcttatttct tggagttaaa
       61 cgcttctgtc ccagcctctt ttttccttcc ttcaattttt tacacgtcga cgattcaata
      121 aaaaaccgcc taatattgta aagacaggtg caagaatcga gaaaaaggcg aatggcaagt
      181 aatgcaacgc gctaactttc atcgttccca taatgaatgt gccgctgact ccccacggta
      241 cgagagaatt gacggtcgca cctgaatccg ctagtgttcg tgtcaaatag gtttttgata
      301 agccaacgtc atcaaaagaa cgcttgaatg tttctcctgg aagaatgatg gacagatact
      361 gttctccaac gatcagattg attccgatac agctcaaggc agtaaacccg actaaacgag
      421 aaggtcggtc catcttttct tttaattctt gaacgatggt atcaacgatc agatatttga
      481 tcagtaaccc acctaatgcc aatgcaagta aaatcaaact agcagaacct aacatactca
      541 tgatcccgcc tcgagaaagc agttcatcta gctttggatc aggtgtattt gcgacataac
      601 cattcatcaa gatgtctgct gcttttccga ccgtcaaact tttctcatgg ataaaagaaa
      661 gaacgagagc agctgtcgat cccgctagta atgtagggat agccggaatt tttttgattg
      721 cccccccaaa taaaataact agtggaatca atgtccaagg agagatccaa aagctcgctt
      781 tcatgttttg gatcatttca ttgatactag ctagatcggc tgaatgactg ttatgtccta
      841 atatcgtata aaaaatcaac gaaaggacga atgctgggaa atccgtataa cgcataccag
      901 cgatatgttc gaatagatta actcctccaa tacccgctgc caagttggtc gtatctgata
      961 aaggcgagca gttattccct aaaaaggcac cggaaacgac tgcgcctgta gtcattgcat
     1021 cattgaatcc aagtaaatga ccgatcccca aaaaagcaat tccaatcgtc gaaatcgttg
     1081 taaatgaact gccgacagtc actccgacaa ttgcacaaac aagaaatgct gtcggtaaaa
     1141 agaaacgcac agaaacaagt ttaaatccat aaaccataat cgtagggatg gttccagata
     1201 aaatccatga agcaactaag acaccaatca aaagaaaaat caggattgga atgatccctg
     1261 gagtgattcc tttgatgatc ccgtcatgga tctcatccca tgtgaacttt ttaaatctgc
     1321 cataaaacaa caaacacatg aaagctgcaa ggatcgggat atgtggcgag agctggaatc
     1381 cgatgatcag tactcctagg agtgccaaca acacgatcaa tacagcgata ctttctttta
     1441 aagaaatatt tttattcatc gtcgatctcc tatttcaaat gaaaaggccc tgccatgaaa
     1501 aaactattca cttggcagga cctcctacta aatcagcgac aaaacgtcgg ctgaaagact
     1561 tattttttta tttttcttct aaactttttt ctacctcaat cccccaagaa tctttcttga
     1621 agtgattgat gatcaatgga ataacgaaca tgaccactgt gatagctgct actaataatg
     1681 catcactcaa tgtcgctgta cctaatgtag caaatacgac aacgatgata gagaagatca
     1741 gcatgcagct gactacccaa gctaatccgt ttccgttttt accaattcgg taaggacgtt
     1801 cgaattctgg atgtttctta cgcattttaa gcaatgaaac agcaatcaaa atgtacacga
     1861 tacaataaat aacagttgta gcatttgtaa gtgtcaagaa tacagcactt acgtcatcca
     1921 ttacaccata aagcaaagca aataatgaaa taacgattga ttgtgttaga acgacatttc
     1981 tagatacgcc gtatttgttt tctttatgga atccgaattt tggtggaagg aatccttcac
     2041 gagctacttg gatgattgtt tttgatggtc cagtaaccca agctgataat tgtaaaagca
     2101 caccaataaa taccatgaag ctgaagatat ttccgatcac ttctggtaag cctaggattt
     2161 gacagtcaat caagataggc tgcgtgatat ttgctaattc catttttcca tctggtacga
     2221 catttgctac aagcatcgca ttgatgacat tcaataaaac taatccgatc aaagcgataa
     2281 atacgccttt tgtataattt tttgttgcgt ctttcaaacg aggcatgtaa acagaactca
     2341 tttcgatccc aacaaaaata aatgtgatac cagctaagta tttcaatgaa tcaatatgtt
     2401 ccaagtcagg gaaagctttt gaccatgaaa agtcacctag atagccgttt gagactagtc
     2461 cgactttgaa ggctgccaaa acacctaaaa cgaacatgat gactactggg atatataccc
     2521 ctagccatac accgatgttc ccgccgactt ttgccatatc gaatttcaag ttcaagatcg
     2581 tgatgatcca gtagataact aaaatacaac ctaaaacgaa ccagtgatta ttgccaagtt
     2641 ctacattacc aaatgtgttt cctaacaatg gtcctaaagt agaagcgacc attaccatac
     2701 ctgggaacat ttgcacccat aatagccatg caacgacaaa tccccatttg ctgcccagtg
     2761 ctgtttttac ccataactga ggaccgcctt cttgaggaag catcgtggaa agctcaccgg
     2821 aaaccattga tatcggaccg gcaaaaaaca aaactgcaaa aattgaataa aaaatcaatg
     2881 tccaaccgac tgcggctaac gtaggaatac tccgaaccgt tgcacacagt gccattgtca
     2941 gaccgataaa ggtcccaaga cccattttac ttacttcaga tttactcacc gttgacactc
     3001 tccttgattt tttggataaa ataacggtgt ctttgaagta gtttcagcaa taaacgaaac
     3061 aattcagcga atatttgagg ggattctttg agtttattgc ttgaagggtt tcttcaaaga
     3121 caacctcaaa acaaagggat cgaagagctt aggtctcccc ttgctcttca gatccaaaca
     3181 tgcgtcagaa acagttagta ttcaagctgt tttctaacat gattaccttg cttattttgc
     3241 ttcgcttgcc aataattggt ctgcgtagat tttttccaat ttttcttgaa gagctgcttt
     3301 gattttttca gcatattctt caaagtgttc ttctttgttc atgtatggtg tcattacgct
     3361 ggcacgcaat acagttactt ttccggcacg gttccattct tcatctgaga atcctagttg
     3421 gttcacaaat tgtaatgggc tgttgccata gtctgggata gcaaagtcag tatgtgaagt
     3481 taagaactca ttaccgtaaa tgcttccttt cacgtatgaa gaataatcat atacgtcatg
     3541 gttcaattta ttcattgcaa ctaagtcatc gttgcctttt tctttgaata catagtctac
     3601 catattgaaa tctggataag ttaatggatg aacttcgatt tctttgtctc ctactttaaa
     3661 tgacaagtca ttcaagaagt tgtagaaacg atgtgcacct tcgattgatg cacccattaa
     3721 tttaccatat cctgtgacat tcaaaggtaa gacatggtgt gctgcccaaa cgctggctgc
     3781 tgttgcccct gcttttgaac cttccaagat ataagcccca agtaatgctg ggatatctgc
     3841 gcctttttca aatacatatg tcgcaaagta agagataacg tctctcatgc ggatatcttt
     3901 gatgacgata ccaccggcag aatatggaac atagcccatt ttatgtgggt caatcgttac
     3961 agattctgct tcttcgattg ctttatatgc actgtgtact tcttctaaga tataatcttt
     4021 gttttctgta aagacattgt atttgtaatg aacatctttt aaatcttcaa atggaatgaa
     4081 gttgttgtct tcgtctaaga aaatcgcacg gccgtatccg ccataagcag catctacgtg
     4141 taagtagaag taaataccgt ctttttctaa tacgcgacgc aatgcaacga ttttatcgat
     4201 accgtcgatt gctccttctt ctgttgatcc tacaacaccg actacaccta ggattggtgt
     4261 tttttcagct gctagaccac gaacgatttt ttcaagttcg ttgatgtcca tgcgatagtt
     4321 gtggtcaact ggcactggaa tcacttggtc taaaccgata ccgatgatgt cggcagcttt
     4381 tagccatgaa tagtgtttag tttgtggaac caaccatttt cctaattttt ctaaatgttt
     4441 tccgctacgt gctgagtgag ctttgatttc gtcgattttt tctgggacgc tgtctaacag
     4501 attcatgatt tcttctgttg acaagttcat taattcccaa tcgctttttc cagcaactag
     4561 ttcaggtgtc acttctttca ttgcaagcgg caatgatttg atgttgcggg cataccaaag
     4621 tccttctaag tttgctaaag aaccatcagc aacgatgtgg ccccagccat ctttatagct
     4681 cattaatttt gcaaattcca ttcctacttc ttcttccatt tggcttgttg caggagaaga
     4741 ttcataggca acgttattac cattccatag catagcaaaa ttgtacgcta gtaaagatgg
     4801 catcaatgtt tcagagttca tatgacccca gtaacgtccg gcattatgcc aaggaacgga
     4861 atgggtacgc atccgcgctg aaatttctga tagaacatcc tttgttttat tcacagtgtg
     4921 ttcaaaacta gcactgcttt tttcttctgg cgtaataata ggcatgtctt gaggcatgta
     4981 gttttgacgc cagcctaaat gttcatccac caattcattc aacaaagctt tatagatttg
     5041 gccgttctca gctttgtccc caatgaatag ggcatttagg tttagatctt tcgacaatga
     5101 ttcactcata gtgaaaccct cctaaaaaaa tatttaaatt ttgtcgagcc ctacattaca
     5161 cctgttcttc tattctgtaa actaagtttt ggcctgattt ttaatcatat tttaatatgt
     5221 atcaattatc cgaattgttc acacttttga aataattaaa aaaaacgagg gaatttacaa
     5281 atgtaaactc cctcgcaaag attgtattaa cgacaaccag cgttctattt taggatgggt
     5341 ttcaaaattg gaatgttgta atagtatttc ccttttttaa ggtaaaaact tactaaattc
     5401 taatcaatta ctttggccaa aaagtaattt ttcttacctt ttcgaataac cacaaactta
     5461 ccatcgaatt catcggatgg atttatgaca aaatttaaat cggtaacgcg gtcaccgtta
     5521 atacttatag ctccgtttga aacatcttca cgtgcttgac gtttagatgg ttcaattttt
     5581 gtggaaacaa gcagttccac gatattttca ggtgtgcttg aaatttcaac gttcggcatt
     5641 ttaccgaatc cttgcgcgat ttcttccgca tttagttctt taatatttcc agagaacaat
     5701 gcttgcgtta ttttttgcgc ttctttcaag tcttcttcac tatgcacaaa tcttgtaact
     5761 tcttctgcca aacggcgttg tgcttcacgt ttctcaggtt ctgtctctac ttttttcgct
     5821 aagtcttcga tttcttcttg agacaagaaa gtaaagaatt tcaagtattt cactacatcg
     5881 cgatcatctt gattcaacca gaattggtag aactcaaatg gtgaagtttt cttcggatca
     5941 agccaaacgg caccgcctgc agtttttccg aatttcgttc cgtcagcctt cagcatcaat
     6001 gggatcgtta aaccgaacgc ttctgcttct ggtccttcta attttcggat taggtctaat
     6061 ccagctgtga tatttcccca ttggtctgct ccaccgattt gcaattgcac atcgtatgtt
     6121 ttatgcagat gcaaaaagtc gatagattgc aggatttggt aagtaaactc tgtaaatgag
     6181 attcctactt ccaaacggct ggcaacgata tcttttgcta acatcgtgtt gatgttgaag
     6241 tttttaccgt agtctctcaa aaattcaagt aaagagattt ttgacaacca gtcgtagttg
     6301 ttcacaaatg tgatatttgc atctttacca aacaattttc tcatttgatt ggaaagtgcg
     6361 tccacgttat gttgcacttg ttccatcgtt tgcaacacac gttctgtttt gcggccgctt
     6421 gggtcgccga tcgttcctgt tccgccgcca atcagaatat atggatgatg gcctgccaat
     6481 tggaatcttt tcatcatcat aaatggaatc aaatgtccga tatgcatcga atcaccagtt
     6541 ggatcaacgc cgcaataaag tgaaatgctc ttttcttcaa cgagctcgcg aagtctttct
     6601 tcgtttgttt gctgattaat ggcgtcgcgc caagttaatt catcaataat attcatttta
     6661 ctgacctccg tgtagttttt ttatgaaagc aggaacctgc tagcatatgt aattaattat
     6721 ttcttcagga taggatagga aataaaaaaa gtcccttggc atataagcca agggacgaaa
     6781 tctcgcggta ccacccaatt tgtatgtcta gatatccaac atacctctcg aacatttaac
     6841 gctgtcgcgt ggcaatcact gccaaagact cctgaaatgt aattcgcttt aaagcatgta
     6901 ctggtttgca gcgaccacca gctttctgaa acattggctt taacaactac ttcgtttcga
     6961 tcttcgtctt gttattatta atatgtgtct atcatagagc gcaacttttt ctttgtcaac
     7021 agaaaaagga tatttttttt aaattcctca ttttttcttt tttgaatccg ctctaattcc
     7081 gaacatttta ttttcaatta ttgttaaaag aaataacttt ttctgtattt tttctgcaat
     7141 ttatcgagaa gtcactttac tttgcctctt attctttctt cgttacaata aggaatggga
     7201 caagtaaaaa aatgaaaaag caagggacga ggtatatgga aaaaaagcaa aaaattattt
     7261 taggagccac ttctgcttta actatcgtta ttttaattct aagtttgttt tttgtcatcc
     7321 gatcatctac aaaaaaagaa ccaatcattc ctgaaaaaaa aataacagaa aaagcaaaag
     7381 aaccgacaga tgataagcaa caactagtta ctcaagcaaa aaatctggct caaagctacg
     7441 attatgacca agcgattgcg ttattggaaa aagatagtaa gaacggatct actgaaacac
     7501 aacagcttct tcaaacacta aaaaaggaga aagaacaact gatcgactgg tctgatccta
     7561 ctcagatctc tcatgtcttt ttccacagct tgatcgtgga cccgcaaaaa gccttccact
     7621 ctcaacaggc acaaggatac aaagattata tggtgactgt tgaagaattc aaccgttcga
     7681 tcgaacaact ttatcaaaat gattttgttt tagttaattt gaatgatttg atccaaaaag
     7741 acgaacaagg aaatctttcc tttaacgggc ttgcgctacc tgaaggaaaa aaaccattgg
     7801 ttctttcaca agatgatgtc agctattatg aatatatgga taattccggt tttcctagcc
     7861 gattggtcgt atcaaataac aatgaaatca aaaatgtcta taaagagaaa gataaagaag
     7921 aaatcggtga ctttgacatg gttcctctga tcgatgcctt tgtcaagaaa catcctgatt
     7981 tttcttatcg aggcgcaaaa ggtaccttgg ccctgacagg ttacaacgga gttttaggtt
     8041 atcgaacatc aaaatctcaa tatggagaca atgaaaaaac aaaaaaagag attaaagaag
     8101 ctaaaaaagt ggcggaacag ttgaagaaag acggctggag ctttgcttcc catacatggg
     8161 gtcacttgaa tatgactcaa gcttcactag aagatgtaaa aaaagacaat gaattgtggc
     8221 agcaagaagt tgcacctatc ctaggaaaaa caaatattct gatttatcca tttggtgcag
     8281 acatcagcga ctggcagcct tattcagcag aaaatcaaaa atttgcttac ttgaagagtc
     8341 aaggtttcga tattttctgt aatgtcgatg cttctacacc agcttgggga caattaggta
     8401 cggatttcta tcgaaatgct cggatcaata tcgatggcat tcgttttcaa tctgatttag
     8461 acggaaaaaa tcctattctt gatccattca tcaatgtcca tgatgtctat gacaaagaag
     8521 cacgcgaatc tgcttgattt tacgtcattt atgaaccaaa aaagccgaac tgacatttta
     8581 tcgtcagttt cggctttttt atgctttatt tcttcttcgg tgcgattccc ttgatcagga
     8641 cttctgtcca ttctgctgta tatggaagca gttttgcgta tatctccggc ttagaaattc
     8701 cttctaccat tgcttgaaaa tagaggatca agaactcatc cgaatatttc aaatccactt
     8761 ttccttcttt ccgccctcga tggaaaagat caagcatgat actgaaactt tcttgcgtat
     8821 acgcatccat cgcgtcagaa aattcttttt tttctttttt tgtatagtat gtcatcaaat
     8881 cgatataaaa ttgtttgttc actcgattga gataacggat tttgttttca ctcatggcaa
     8941 tcagtgtttc ttcaaatgga agattttccg caatcaacgc tttcgcttct tgccctaaat
     9001 cattcataaa tgcaaaaaat acttcataaa tcagcccttc cttgctgcca aaatatttaa
     9061 aaatcgtcgc tttacttacc tgagcgttct cagctacctt atccattgtc actccagcgg
     9121 ttcctatgtc tgtgttcatc aagtcaaaag ctgcttgtaa aatctctttc tttttctctt
     9181 gtgctctctt ttcaaaaccg ttcatttgta gagcctcccg taattttaaa cttttataat
     9241 atgtatagtt caaatttatt ctgaggttta ttataactat ctattgagaa agtatcaagc
     9301 tagcaattcc ttgtttatga attcattgac agaaatttat aaaaagaata ttatgtacta
     9361 aaataataaa aaaagtttta taaaaaggta taaaaccttt ttattaaact atttaatctt
     9421 gtttagtata tatttttaga aaaaggagtg gggtttttat ggcagaaatc gtcaaagtca
     9481 cacatttgaa aaagagcttt ggtcgtttcg aagctttaaa agatgtctct ttcaccgtac
     9541 attcaggtga agtcgttgga tttatcggac caaatggttc tggaaaatcc acgacgattc
     9601 ggacactttt agggattata aaaaaagacg gcggaacggc aacgattttt gggcaagatg
     9661 tatgggaaga tagtttatct atccatgaac gtctttctta tgttccaggc gatatctctt
     9721 tgtggggaaa tttgactgga ggagcgatca tcgatctctt catgaagctt catggaaaag
     9781 ggagtgctca aaaaaaagat gaactgattc aacgtttctc tcttgatcct aagaaaaaag
     9841 caaaaaacta ttcaaaagga aatcgccaaa aagtgggctt gattgctgct ttggctgttg
     9901 attcggatct ctatttattt gatgagccta cttctggttt agatcctttg atggaaacga
     9961 ttttccaaga agaagttgaa aaattgaaag aacagggcaa atccatccta ctttcttctc
    10021 atattctcag tgaagtcgag cgactagcgg ataaagtcgt gatcatacgc gaaggaacac
    10081 tcgatgaaga aggaacactc gatgaattgc gtcatttgac tcgttcgacc atcactatag
    10141 aaacagaagc agagaatagt cacttagcac aattgaacgg tgtccatgac tttgtgcaaa
    10201 aaggtcacct cgctcaattt gctgctgata acgaagcttt cgatgtgatt ttagctgaag
    10261 cgtccaaatt aggtgtgaaa aaatttgaat ctattccacc gactcttgag gatttattca
    10321 tgagacatta tgaaggatga ggaggaagcg aaatgactga taaattttca cgctggtcca
    10381 cccttttttc tcaaaacctt agccgtgact ggaaaaagat cctgatatgg ctagtcggtg
    10441 tcactgcttt ttctggtggt tttgtccctg cgtttgaaga attgacttcc ggacaaggat
    10501 tagctggatt atacgaaacc ttgcaaaacc cggcaatgat tgcaatggtc ggaccaactc
    10561 cagcttcgaa tgctgctgag tatactttag gagcgatgta tgcaaatgaa atgttgcttt
    10621 tttgcagttt gtttgctgct gtgatctctg ttttacacgt ggtgagccaa acgagaaaaa
    10681 aagaagaact tggtctgatt gaacttattc gttcctttcg agtcggacgt caagcaaact
    10741 ctttagctgt catcgctgaa agtattctga tcaatttgct tttagcaata tcgatcagct
    10801 gtacgatgat ttttttccaa gcggattcga tcactgcaga aggtgcttgg ctttttgggc
    10861 tatctgtagg tagtatgggg attttgggga gtgccgttgc actgtttgct gcacaactga
    10921 tgccaagttc agccagttcg aatggtctgt ctttagcgtt tttaggtgga ctttaccttc
    10981 tacgagcaat aacagatgta gctgattcct ccctttcgat gtggaatcct ttaggctggt
    11041 cttatttgac ctatccattt acaactaaca actggattcc tttgctttat ttgtttttgt
    11101 tcagtctaat cttgataatc agttcatttg ttttagagga acatcgagat ttgaacaccg
    11161 gctatatccc cgaacgtgaa ggaaaaccgt atgctaaacg ttctctttta tccttgcgtg
    11221 gtttattatt ctatctaaat aaagggatga caatcagttg gcttatcact tttggtttga
    11281 tgggggcggc atacggctct atctacggga atatgcaaca gtttttagaa agtaacgact
    11341 taatgaaaca gatgtttatc caatctggcg tctcaattga aaaaaacttt acaagcacga
    11401 tcatggtagt cttgatttgt ctagctgcta ttttaccaat tgctagcatc aatcgtttat
    11461 ttacagaaga aacccacgga cgtatgaatc agctgttcgc aacaaaaatt tctaggaacc
    11521 aactttattg gaccaatatg ttactagcta ttctatttgc tatattggca atcctgatta
    11581 gttctggtgc actagggctt tctgctttgg cttcgatgaa ggatgcgaaa gacatggatc
    11641 tattgatgtt tcttaaagcc gggttcaatt tcctgcctgc cgttttagtg tttatcggaa
    11701 ttagtggctt tctattcgga tggcttcctc gaatcggtaa acttgcttat atctacttag
    11761 cttactcctt tgcactcaat tactttggcg gaattttgga tttaccagaa tggttttcca
    11821 aaacagctgt ccctagctgg gttccacgga tgccaaacga ttcttttgat ctttccgtgt
    11881 tccttacgtt atcatgtatc agccttgcac tgttcgtcct tggttctatt ggttataggc
    11941 gaagagactt actagaagga taataaaaaa gcatgtacaa tttcatgcta gcctccaaat
    12001 ctgggtcaaa aatccaacat ttggaggtca tttttttatt tacaagtctt ctaactgctc
    12061 tttatcaatg atccagactt ctcgattctt tctttttaca taaccattct cctctaataa
    12121 tttaaatttt ctagataaag tttcgggggt cgtaccgata aatgcagcaa gttctttcat
    12181 tttcattggc aggaaaaagt gatccttccc tgtcgcttta gctaagtcaa gcagatagac
    12241 agcgactcgt tcttctactt tttccatcat caaaaaagtt gtctgctgct gtacttttgt
    12301 taatttctca acgttgattg ctaaaagttt cagactaagg gcgggataat gcagtaagag
    12361 ttccgagaaa tctttttgtc tcaatatgca aatttccgtt ttttctagtg cttcgccaaa
    12421 taaagactca tttggaacgc ctagcaactg gctctctccc tcatatccgc ccggctctat
    12481 tactcgtaaa agctgttcct ttccgctagc agataattga tacactttca tactgccgga
    12541 ggccacgatc actaattgag gttctccatt cggcgcaaga atctgttctc ctttttccac
    12601 atagtgatgt ttgacaagtg cgtgtacttt ttcttgatct tgctgtacta agtgattgaa
    12661 caaaggaaca aatgaaacac ataaatgatc atgactcata atctctcctc cttctaaaca
    12721 gctatttcta ataaagaaac ataacttccc ttattccaac tgataatttt cttttatcat
    12781 agcataaacg tcaagcgaac cagataccag caaatagagt ggatttcttt ttgtcccttg
    12841 ctttattttc cttcttttga gaccttaagt aattgattcc tatcaatttt ttaaatattt
    12901 actttttgaa taatcaaaaa caaaccttca ctcgcaagcc gagagtaagg tttgtttttc
    12961 gcattgctta tttcgtgttg tctattgaaa tttttccgca cctagctttt gatatagtgg
    13021 tgctagttct tgtaaggttt cagcggttat ttgctcaacg tgttcgattt cttgcggata
    13081 gaagaatagt ttacccattt gaaaggttcg gtctctcatc gcttttttca actcttcttg
    13141 tttctttgta tcggacaaat agtcagacaa agctaagtgg tcgtcaagtt catgttctgg
    13201 ttgcggccat atgtaataat ccttgatatc gactgttttg ctccactctt tcagtatcgc
    13261 aagcaactgg ttatacgctg caatgctacc atatcgttct tcgcttttat aatgttggta
    13321 catcaaatag atttggtatt gtttttgatt caacaaaact gctagacagg cattctcgtc
    13381 ttgacgagat tcgcttcggt acgcgctcca aaaatgactg cgtaaattcc agccattcgt
    13441 ccagctttcg attttaggtt tgctcatgcc gtacatatca ggcaaaagtg cagctgtctg
    13501 gagctgaatc tctttccatt tttgccagtg ttctttatat tctttcttga tttgttcaat
    13561 cttatctgtt ggatggctct ctttgagctg tttgaatgcg tagattggtc gatcaaacaa
    13621 ctgataatct gattttccaa gttccattgt ttcgcctctt tccgttatct tttgtattct
    13681 acctgaattt tgcgatatgg acaaatagag gttgtgaaaa agctgttctg catcaagtca
    13741 taagaacagc caaacaaaaa actgcgatga gcatcaccgc agtttt
//
LOCUS       DAMEET010000072        13299 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.073, whole genome shotgun sequence.
ACCESSION   DAMEET010000072 DAMEET010000000
VERSION     DAMEET010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.073"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(9..530)
                     /locus_tag="PB780_001966"
     CDS             complement(9..530)
                     /locus_tag="PB780_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDE0734014.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            729..4421
                     /gene="nifJ"
                     /locus_tag="PB780_001967"
     CDS             729..4421
                     /gene="nifJ"
                     /locus_tag="PB780_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDE0734015.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            4610..5497
                     /locus_tag="PB780_001968"
     CDS             4610..5497
                     /locus_tag="PB780_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDE0734016.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5494..6663
                     /locus_tag="PB780_001969"
     CDS             5494..6663
                     /locus_tag="PB780_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734017.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6687..8042
                     /locus_tag="PB780_001970"
     CDS             6687..8042
                     /locus_tag="PB780_001970"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDE0734018.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(8114..9919)
                     /gene="glmS"
                     /locus_tag="PB780_001971"
     CDS             complement(8114..9919)
                     /gene="glmS"
                     /locus_tag="PB780_001971"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDE0734019.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINCLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            10328..10618
                     /locus_tag="PB780_001972"
     CDS             10328..10618
                     /locus_tag="PB780_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734020.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            10631..11656
                     /gene="sppA"
                     /locus_tag="PB780_001973"
     CDS             10631..11656
                     /gene="sppA"
                     /locus_tag="PB780_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDE0734021.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEKGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            11659..12318
                     /locus_tag="PB780_001974"
     CDS             11659..12318
                     /locus_tag="PB780_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDE0734022.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            12475..>13299
                     /locus_tag="PB780_001975"
     CDS             12475..>13299
                     /locus_tag="PB780_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734023.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLT"
BASE COUNT         4215 a         2445 c         2930 g         3709 t
ORIGIN      
        1 ttattttttt attttgtttg gatcaattct tttaattcct ctattgaaat agattcatca
       61 aagatttctt tacccaagat gacagttgga acaaatcgaa tatgtgcttg ctcagcttcg
      121 ttgatgacag cttggctcag ctgttcgtct ttttgttctg tcagctttag tttatctact
      181 gcaaaatccg ctacttcttg gagagaaaga tgtttccact cgtcttgagt atcgaatatt
      241 tgtttgatct ctttgatcgc tttttggcca tcgctgattg tgagataccg gtgcatcacg
      301 ttccctcgct gtaagctttc tttttcttta tcgaacaatt tgatcactcg ctgcaattgc
      361 ccgctttgga ctgcttcttc taatagctca taagattctt caaaccattg tcggcaatac
      421 ggacaagcta agttgatgaa ttcgaccatt tttttaggag cattcgatga accataaagg
      481 attccttttt gtgtatttgt tttcgttgca tcaatcactg atatatccat gttaaaacac
      541 ctctttcgct tatagtttat acatgtctga caaaaaaagg caacgattta gactgtttgt
      601 ctaaaaatta gatgaccaat tcaaatagat acatgtaaaa tatttttata aataaaagaa
      661 aaaaatggca tgttttacgt tataatggaa cagataataa gagagaatat gaacaaggat
      721 gatgaatcat gcgaaaaatg aaaacaatgg atggcaatac ggcagccgca tatatttctt
      781 atgcatttac cgagattgct gcaatatatc caataacacc cagttcgacc atggctgaat
      841 tagtagatca atggtcagca gcaggaaaag aaaacgtatt cggtcaacca gtaaaagtag
      901 tagaaatgca atccgaagca ggagcagctg gtgtggtcca tggatcattg aaaactggtg
      961 cactgacaac tacatatacc gcttcacaag ggctgctttt gatgataccg aatatgtaca
     1021 agattgctgg ggaactactg ccaagtgttt tccacgttgc tagtcgggca gtgacgacaa
     1081 atgcgttgaa tattttcgga gatcacggag acgtgatggc tgcaagacaa acaggatttg
     1141 ctatgttagc tgaaagcagt gtacaagaag tgatggattt agcacctgtt gcccatcttg
     1201 cttctattga aggagaagtc ccttttttga atttctttga tggattccgt acgagtcacg
     1261 agattcaaaa aatcgaagtt ttggaatatg aagaattagc tcctctgatc aatcaggaga
     1321 aattagcttc atttcgtaaa cgagggatga atcccaatca tccgactgtt agtggtacga
     1381 accagaatcc agacatccat tttcaacaac gggaaacgat caacccctat tatgataaat
     1441 tacctgaaat cgttcaaaaa tatatgaaag agatcaaccg attacgagga acttcttatg
     1501 acttggtgac ttattacgga gcagacgatg cggaagaagt cattgtttct atgggatcag
     1561 ttgcacagac gattgaacaa acgattgatt acttgaatgc aaacggacgg aaaacaggtt
     1621 ttttgaatat ccatctttat cgtccttttc cagtggaaac attcgtgcaa aagttgcctc
     1681 atacagtaaa aacaattgct gttcttgatc gaagtaaaga accaggggca ggtggagaac
     1741 ctctcttatt agacgttcaa agtgcgttat atgattctga acttcgcccg gctgttattg
     1801 gtggtcgcta cggattgggg tctaaagatg tgactcccga ccaaatctca gcagtttttg
     1861 atgaattgaa aaaagaccca agcatacgca aaaaacggtt taccataggg atcgtggatg
     1921 atgtgactta tcaatctttg gaaccaaagg aaagtctgga tttgacagaa ccgcaaacat
     1981 tccaggccaa gttttgggga tttggatctg acggaacagt tggagcaaat aaatcagcta
     2041 tcaagattat tggagatcac accgataaat atgcgcaagg ctatttttat tatgattcga
     2101 aaaaatctgg cggtctgact gtctctcatc ttcgttttgg agatacgccg atacgttcag
     2161 cgtatcttgt cgagcatgcg gaccttgttg cctgtcacac accagcttac ctgcatagct
     2221 atgacttggt caaaggattg aagccaggtg gcattttttt attgaatact ctttggagcg
     2281 acgaacaatt agaaacgcat ctgcccttaa aattaaaacg ttacttggct gaaaataaca
     2341 ttagattcta tacgatcaat gccatgagat tggcccaaga agtcggtttg ggacgccgga
     2401 tcaatacggc aatggaaacg gcttttttta aattagctga catcattcct tttgatgagg
     2461 tgcttccgct gctaaaagaa gaagccctga aaagctatgg acataaatca atggctatcg
     2521 tagaaaaaaa catacaagca atcgatcgga cgaccgaact gcttcatcaa gtcgcgatcc
     2581 cagaccattg gagaacgttg gagatgcctg aaaaaatcaa taaaaggaac actggttata
     2641 tacaagaaat acttgaacca gtgaatgcac aggaaggaaa tcaactctct gttggtacac
     2701 ttgtagcaaa tggaatgaca actggcgaaa tgcctttagg aacggcttct atggaaaagc
     2761 ggggcgtcgc tttagaagta cctgagtgga ttagtgatcg ctgtacgatg tgtaatgaat
     2821 gtgcctttgt ttgtcctcat gctgccatcc gtccattttt agcagatgaa gaggaaatgg
     2881 aagaagcgcc agaaggattt atcgtgagag aaatgcgagg cgcagacggc gtaaaatacc
     2941 gtatccaagt ttcagtggaa gactgtacag gttgtggtct gtgtgtagag gcttgtcccg
     3001 caaaagaaaa agctttagtg atgaagcctt atgaagaaca gaaagaacaa gctgtgaatt
     3061 gggcttttgc aatgacatta agacaaaaag caaatccagt caaaggaaag aatactgtct
     3121 tgtctacaca attcaatcaa ccgcttcttg aattctcggg cgcttgctca ggctgtggcg
     3181 aaacacctta tgtaaaatta ttgacacaaa tgttcggtga tcgaatgatg atcgcgaatg
     3241 cgacaggctg ttcttctatt tggggcggtg cttctcctgc ttctccttat acgaccaatg
     3301 aatgtggtca agggcctgca tggtcaaatt ctttattgga agataatgct gaatttggtt
     3361 atggtatgct tctagcggca cagactcgtc gcgaggcttt ggcaagttca atcaaagaag
     3421 tattgccaaa agtatcagaa gaactacgag aattgatgga agactggtta gtacacatgt
     3481 ttgagagtga aggaacgcag caaagagcag caaaactgca agctgcgatc gaaagcgaac
     3541 gatcaatttt ccctgaactg gaggatatat acaagagaaa ggatctattc gtaaaaaaca
     3601 gccaatggat gattggtgga gacggatggg cttacgacat tggttttggc gggatcgatc
     3661 atgtacttgc cagcggagca gacgtgaata tccttgtttt ggataacgaa gtctattcca
     3721 atactggcgg tcaaacctca aaagcaacac cagcttcggc aatcgctaaa ttttcggcta
     3781 gtggaaagta tgcttcaaag aaagatctag gaatgatggc gatgacatat ggaaatgttt
     3841 atgtggcgca aattgcttca ggtgccaatc aaatgcaaac gatcaaggca tttgaagaag
     3901 cagaaaagta tcctggtcct tctctgatta ttgcctatac gccttgtatc acacatggct
     3961 tgcttggcgg aatgaaagaa tcgcttaaag aagcaaaaga agctgtttct tctggttatt
     4021 ggtcattata tcgatataac cctacaatgg tcgaaaaaaa caagaaccca atgactttgg
     4081 actacaaaaa acctgatttc tctgctatgc ctgatttcat gaggaaacaa gttcgctttt
     4141 cctcgctaga taacgcaaat ccaaatcttg caaaagcttt atttgaaaag actgtccacg
     4201 acgctaaaac acgtttttac aactatgctg cactgacggg agaattagaa aaaatcaaga
     4261 aacgacttga accagaggaa tctatcgatg tacccgttaa gactagaaaa gaaaaaacga
     4321 cggaccttga agcagaagaa agaagagctg ctagaagagc tgccagagca gaacgacgta
     4381 aaaaaatgga acaagctaca aaagaaaaaa aggaaaaata acagaaaaaa tataatgaat
     4441 aattctctga agtttaaaaa tatttattgc agagaaaaat aaaacgcata cttgcaattt
     4501 tcttgaaaat tatgcgtttt tctcaagaaa aatacgagca tatcagcctc taaaattaaa
     4561 acatatgata tactagagag gaaatctcaa aagagaagag aggcttttta tgtcatttca
     4621 aatcggggaa ctttttaatt tggactattg gcagcaaatg ttttccggtg acttgttctc
     4681 tctgaatttt ttggtgaaca tacttgatat tctggttgtc tggtatcttg tatacaaact
     4741 gatccaactg ctgcgaggca caaaagcgat tcagcttctg aaaggtgtag ctatttttat
     4801 tgttatccga atcgtagcgg aaatcattgg tctgcacacc ttgtcttggc tgatgaacca
     4861 agtcattacg tacggggtga tagctgctat cgtgattttt cagccggaaa tccggagagg
     4921 actggaacat cttggacgca gttcgctttt caaacaatca aaaagcgaac agcaggaaga
     4981 tgagaagatg atcttgtctt ttgacaaagc catccaatat atgtccaagc gaaaaatcgg
     5041 tgcgttgatc actatcgaac gtcatactgg attagatgag tatattgaga cagggattgc
     5101 tttagatgca gatatcactg gggagctgct gatcaatatc tttattccta atacaccgct
     5161 gcatgacggt gcagttattg taaaagaagg aaagatcgca gtagcaagcg cctatcttcc
     5221 tctatcagaa agtatgttga tccctaaaga attcgggact cgacaccggg cagctgtcgg
     5281 tataagcgaa gtcagtgacg cgattaccat cgttgtctct gaagaaaccg gggacgtcag
     5341 catcacgtta gataatgaac tgatggctgg cttatcccag caagaatatt tggcaatctt
     5401 gcgtagagaa ctaattgctg aagagaaaac aaaaaacaag aaaaacgtga ttcaatcttt
     5461 cctagacggt atgactaagg gaggggatcg caaatgatct caaaagagaa acggacaagc
     5521 attctttaca gttttattgc attgctgttc agtattgcgc tgtacttcaa tgcaaatggc
     5581 cagtccgttc caaatacgct ttctggaaat gaagcgtata atcaaacagt tacgggggtg
     5641 ccgatcaaga tttcttatga ttcaaagcgc tactatatcc atggatatga aaatgcagtg
     5701 actgtcaaac tttccagtgc aaaccgagtt cagttgaatg cagaggcaaa cgaagataca
     5761 cggatgttcc gcgtcacagc agatatcact aagctgggcg aaggaacaca tgaagttccg
     5821 ctaaaaattc aaaatttgag cagtgctgtc acagcaaaga tcgaaccatc gactattaca
     5881 gtaacggttg agaaaaaggt aataaaagat ttcgatgtgg aaacgttgat tccttcaacg
     5941 ttcacaccat ctggatatga acttgatgat acttccgtta gtcctaaaaa agtctctatc
     6001 actacaggtg acaaaacatt ggcagaaatc aaacgagtag tagctgatgt ggacgcttcg
     6061 atggtcacag atgatgggat taatagtgag gtcccaatac aggcgctgaa tgcagcaggg
     6121 gaagtattaa gtatcgtctc tgatccagtg caagtaacag tgagagctga ttcgatcaag
     6181 ccaagcaagt cggtacgact ttatgggata cagcaaggaa caccagctgc cggggtagaa
     6241 agctatgatt tcagtttttc aaaactggaa gcagaagtaa gcggctctag cgatttgctt
     6301 gcatcgatcg gtgacagtat tgctgtaccg atcaatgtct caggtatcaa tcatcgaaca
     6361 actcgtaaaa ttgaaatacc agtagaagag gggttaagta tcaagcccga atcagttagt
     6421 gtggagatta cacccgtgtt gcaaacgtca agcagtagct ctgcttcgac gagcagtcgg
     6481 acacaagaaa atacaagtac gagtagaagt atttcatctg ctgattcagg aacggaacca
     6541 tcgacaacaa ccatttcttc cagttcggaa tctgggacaa gcagctcgac cacaagtaca
     6601 gaagaaacat cagaaacaag cgaatcaaca caaatgagca gaacaaattc ctcagaaaat
     6661 taagttaaag gagtaatttt acaactatgg gtaaatattt tggaacagac ggcgtacgcg
     6721 gtgaagccaa caaggaactt acgccagaac ttgcatttaa gctgggacgg tgcggaggtt
     6781 atgtcctaag ccagcatgaa gaagaaggaa aacgcccacg cgtgttagta gggcgggata
     6841 caagaatctc aggtcaaatg ttagaagaag cgctgatttc aggactttta tcggtgggta
     6901 tcgaagtgtt ccagctaggt gtgatctcta caccaggtgt tgcttatctg acacgtttgc
     6961 aaaaagcaag cgcaggggtc atgatttctg cttctcataa tccagcacaa gataatggaa
     7021 tcaaattttt cggtgcagat ggttttaaac tagtagatga ccaagagcta gaaattgaag
     7081 cattattaga tgctgaagtg gatgaattgc ctcgtccatc agctgaagga ttgggaactg
     7141 tagaagaatt tccagaaggc ttattgaaat attctcaatt ccttgtacaa acgatcaacg
     7201 gtgacctttc cggtttgacc atttgtgtcg atgcagcaaa cggagcaact tcaacaagtg
     7261 ttaatcgttt gtttgccgat cttgaaacag atttttatac aatgggcaca aatccagacg
     7321 ggttaaatat caatgacggc gtaggctcta cccatccaga acgtttagct gagatggtcg
     7381 ttgaaaaagg agcagatgct ggactagctt tcgatggcga tggcgatcgt attattgcgg
     7441 tggatgaact tggaaacatt gtcgacggtg ataaaatcat gtttatctgt gctaaatact
     7501 tggcagaaaa aaatcgattg aaaaaagata cgatcgttac gactgtcatg agtaacttag
     7561 gattccacaa agcggtggaa ggaatcggtt tgaaagatgt cgttacacaa gttggtgatc
     7621 gttacgttgt agaagaaatg cgcaaaaacg attataactt tggcggcgag cagtctgggc
     7681 acatgatttt cttagatttt aacacaactg gagatgggat gctttcagga attcagttac
     7741 tgaatatcat gaaacagaca ggtaagaaat tatcagagct tgcagcagag gtaacaatct
     7801 atccgcaaaa attagtgaat atccgcgtaa gtgataaaaa cggtgcgatg gatgttccgg
     7861 caatcaaaaa agtgattgaa gaggcagaag ctgaaatggg atcagaagga agaatcctag
     7921 ttcgtccttc cggtacagaa ccattacttc gagtgatggc agaagcgcct actgatgaga
     7981 aagttaatta ctatgtcgat aaaatcgcgg cagtagtcaa agaagaaatc ggactggatt
     8041 aagagatatt ttcataaaaa ataaaagaac aaggaccagt gtcagatgtc tgacactggt
     8101 ccttgttctt ttattattct actgtcactg atttcgctaa attacgtggt ttgtcaacgt
     8161 cgaatccccg taatagcgtt gcgtaatatg caagtaattg agtcggtacg actgtggtta
     8221 gagtagttag ataagggtgg acagttggta gcaccagctg gtcactttcc ttttctaatc
     8281 cctctgagac aataactagt gttttagcgc cacggctttc gacttctttc aggtttcctc
     8341 gagtatgcgc accagtcact tcttcactga tgattccgat cactggtgtt ccttcttcga
     8401 tcaaagcaat cgttccatgt ttcaattcgc cagcggcaaa accttctgcc tggatataac
     8461 tgatttcttt caatttcaaa gcagcttcaa gagaaacgtt gtagtcagca ccacgtccaa
     8521 tgtaaaaggc atttcttgta tcgctgagat atacttctgc caactcttct aaataatctt
     8581 tttgggcgat gaccgattcc atcgcattag caactaaact caattcatga gtgatatcga
     8641 atacaagaga agtttcaaat tcttttttat caccgatagc tttggctaat aaagttagta
     8701 cagctatttg tgcagtataa gctttcgttg atgctactgc gatttcgggt ccagcatgca
     8761 atagcagtgt aaaggatgct tcccgcgaaa gagtagaacc agcaacattt gtaatcgtaa
     8821 gtgaagggta gccgagacag ttgattttta ccagcacttg gcggctgtct gctgtttcgc
     8881 cactttgact caagaaaata aagaatggtt tttttgaaag aagaggtgtg ttatagccaa
     8941 attcactggc taaatgaacc tcaacaggaa tctgtgtcaa tttttccaaa gtctgtttac
     9001 ctgctaaccc cgcatggtaa ctagttccgc aagcaacgat atagattcga tcgctatcag
     9061 acaatgtatc gaccagcttt tgatcaagag agacacgatt gtcttctgtt acgtattttt
     9121 gagcgatacg acgcatgata ataggctgtt cgtcgatttc tttcagcata taaaaaggat
     9181 aggtaccttt ctcgatatct gaagcatcta cttgtgcttc gaatgaagaa cgttctacga
     9241 tgtcacctgc aaaattttga ataataactt tttctgatgt gacggtaacc atctcttcat
     9301 cttcaatctc aacgaattcc ttcgtatgag caagcatagc cattgcatcg ctggcaatca
     9361 cattgaatcc atcgccgagt ccgattaata gtggactttt atttttagcg acgtaaataa
     9421 catcaggtgc tgtccgatca atcaaagcga aagcgtaaga acctttgatg acccgcaaag
     9481 cttttaaaaa cgcctcttgc gttgtagtat ccttcgcgaa agtttcaatt agatgagcga
     9541 taatctcagt atcggtctca ccaataaagt ggtcatttgc taaataagca attttaagtt
     9601 cttcaaagtt ttcgattacg ccgttgtgaa cgagcacgaa gcgtccactt tgggaagtat
     9661 gtggatgggc gttttcttca gaaggttgcc cgtgagttgc ccatcgtgta tgaccaatac
     9721 ctatatttcc tgtagtatca atatttattt tgtttttcaa attttgaatt ctgccttgtg
     9781 ctttgaccag ataatcagtt gtgccatctg agacaaagat accagctgaa tcataaccac
     9841 ggtattctaa tttttctaga ccgtcaatca atcctggtgt tacatttttt aatccaatca
     9901 ttccaacaat tccgcacata aaaaacatcc tactttctat aatgaattcg tatgaatgct
     9961 tccagctgca ggaaaatcct tttgttttat ctttgcagac attttttgtg gattttctgt
    10021 agagtgcagt ccctagggcc atccgccgaa tctttcgata aaccctctcc tcgtcatctg
    10081 tttacacaga cctggcgcta tcctaaaagg tataactatc ctcctttttg ttttttatct
    10141 ggaaacattt aaaatcataa ccttttgcga atgttcttgt caacaaaaaa agaataaaaa
    10201 ataaattggt atatatcaaa ttctttgtca aatgtccttt ttatgtcaaa aaataatggg
    10261 tttttatcta tcatcttttt caggagttcg gtacaataga aagtaatcgg atagaggagg
    10321 agtaaaaatg gattcaaaaa ttgagatcat gacactggga atgttaaaaa aacaattgtc
    10381 caaattcgaa gcatcagctg gtgtatcaga tgatacgaaa atatttttag atactggctg
    10441 ggatagcatc caagaaatcg cacctgatgc cttggaagtt gctcaagctc gtgagtttac
    10501 ggtagaagat gaattaacaa aagagtcgtt ttccgggtat gcgcgggaag aaaaagcaga
    10561 gcggttcgac acttctgagc agtcagaaac tgtgatcgtc ataaaaaact tgtattagaa
    10621 aggagaaaga atgaataaaa gacgttgggc agcagttctg attgctgcag gattatttgt
    10681 ggtttcgtta gtttcagcta gtttgactaa gcctcaggaa aacgatcaaa ttgagggatt
    10741 gaacagttgg ttatatgggg atgaagaact agctccaaat gtgttggaaa agggagacag
    10801 tacgcaaaaa atcgtaaaat tatcagtaga tggaacgatt gctgcgggag ggtcttcagg
    10861 tttatttgct tctgaaggtt acaatcataa aaattttatg gaacaactga aggcgattga
    10921 agaagatcca acagtcaaag gtatcttttt agaagtaaat tctcctggtg gcggtgtcta
    10981 cgaaagtgcg gaaatcgcta agaaattgga tacgattcgt aaagaacatg atatcccaat
    11041 gtatgtcagc atgaaaaaca tggcagctag cggtggctac tatatttctg ctcaagcaga
    11101 taaaatcttt gcaacagaag aaacagtgac cggctcaatt ggtgtgatca tgtcaggatt
    11161 gaattattca ggacttctag aaaaactggg tgtggaagat acaacagtta aaagcggagc
    11221 gttgaaagat atgggttcag gaacgcgacc agagacaaaa gaagaagaag cggtgcttca
    11281 agcatatatc gacagcgcat atagtcgttt tgtagcaatc gtcagcgagg gacggaataa
    11341 atctgaagaa gaagtcaaaa aaattgctga tggaaggatt tatgatggtg tccaagcaaa
    11401 agaagtaggt ttagtcgatg aattgggatt tcctgaagat gctttaaaag ctatacgaaa
    11461 ggaacaacag cttgaagatg cggaactagt agaatattct tccacgccat ctgggtttgc
    11521 tagtacatgg ttaggaagta aactagcaga attccaggga ttgaagcctt ctgagactag
    11581 tcagatctta tccgttcttg agaatctagg gacagcggaa tcaccaaaag caatgtatta
    11641 ttacggaggt gagtaagcat gtcagagctt tcaaatgaaa ataaggaaca agaaaaagat
    11701 aagcaacagc agcctgctgt aggacctgac tccaactttt cccaaaaggt actgagctct
    11761 tttgaaagag agcctttgac gcccgaagaa atcaaaaaga aacaacgtaa atggaaaaag
    11821 tatacggaca caagaaatga accgccaaaa gcaaatgatt ttcctaagta tttctatgca
    11881 ggttttttta tccgaatgtt tgccttcttg gtcgatcttt tgtgtatcgg agctattacg
    11941 cgtttaacac ttggtattgc caaaaatcta ggttggctta atttttcaga ttcttatctg
    12001 agtctctacg gattcacagc cttacttatt tatctagcgt attttatttt attgacgaaa
    12061 ttcaatcatg gccaaacgat tgggaaaatg atctttggta ttcgagtcat cagctttgat
    12121 gaagaagaac ttagctggtc aaccgtttta gttagagaag gtgcttgccg gttcattcta
    12181 aaatttcctt tgctgatgtt agggtatctt ccagcagtct ttaatcagaa aaagcaacat
    12241 ctgggcgatt ttttcactaa tacaagtgtt gttacgatca atctgatcaa agcgttcaac
    12301 caggaagtca acagctgatg agcaataaaa agcagtaaca gttgaaagca ccttctaaaa
    12361 gtcagatcta aaatctagct tttagagggt gcttcagtta tttttaattg tcatgctttt
    12421 tttgttttgc tactattgaa catgagctga atatgagcaa gaggaggaca ctgcgtgaag
    12481 ccgattgatt ttccagaaaa atatgagaat ttaatgcgag tggcacaaca agcactagca
    12541 aaccagcaat atcagcaggc aaaagaatta tttcaacgag cttacgagtt gaaagaaagc
    12601 tttgaggcta atagcctgct tgttttttgt ctttacgagt tggacgagaa aaaagaagca
    12661 ttgaagcaag cactcttgca tgaaaaacag tatcttcaaa atgaggagtt tgctgaattt
    12721 tattttgact tgttaatcag tacacaagat tttttatatg ctagaaaatt gatagcaagt
    12781 acagattttt atgaagcttt cgagcagcgg gtcattgaaa aaatccaatt tgcagaagaa
    12841 ttatccggac aaatggagag gcagaagatc aaggcactcc acaataaatc ggaggaactt
    12901 ccttcgttgg aacctgcacg tcagcttgcg tctattgatg agatcgaaca gcttccttat
    12961 catgagttta tccaaacagc gagtaaactt attgtatcgc cagaggtcca tattttagca
    13021 agggcaaaat tactggagac acttaggcaa ttgaacgaat gccatcctgt tttctatttg
    13081 acgattgaag aaaagttagt caaagtcata ccaaaagatc tgccaaagcc gcagcagcag
    13141 gcaagttacc agcaattatg tgtattttca gatcgttacg ggaatgaaga tgcgctgctt
    13201 agttccgtgt taaaagaaga attcacgttg caatctgcaa ttgtttatcc tgtctatgat
    13261 acatacatcg aagacccgaa gcgctggttt caactgact
//
LOCUS       DAMEET010000073        13146 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.074, whole genome shotgun sequence.
ACCESSION   DAMEET010000073 DAMEET010000000
VERSION     DAMEET010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.074"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(77..149)
                     /locus_tag="PB780_001976"
     tRNA            complement(77..149)
                     /locus_tag="PB780_001976"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="PB780_001977"
     CDS             530..1099
                     /locus_tag="PB780_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HDE0734024.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="PB780_001978"
     CDS             1086..1946
                     /locus_tag="PB780_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDE0734025.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2136..2840
                     /locus_tag="PB780_001979"
     CDS             2136..2840
                     /locus_tag="PB780_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDE0734026.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="PB780_001980"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="PB780_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HDE0734027.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /locus_tag="PB780_001981"
     CDS             4677..5993
                     /locus_tag="PB780_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734028.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="PB780_001982"
     CDS             5994..6863
                     /locus_tag="PB780_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HDE0734029.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="PB780_001983"
     CDS             6918..7727
                     /locus_tag="PB780_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDE0734030.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="PB780_001984"
     CDS             complement(7830..8459)
                     /locus_tag="PB780_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDE0734031.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="PB780_001985"
     CDS             8629..9921
                     /locus_tag="PB780_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDE0734032.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9997..10659)
                     /locus_tag="PB780_001986"
     CDS             complement(9997..10659)
                     /locus_tag="PB780_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDE0734033.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10884..11168
                     /gene="groES"
                     /locus_tag="PB780_001987"
     CDS             10884..11168
                     /gene="groES"
                     /locus_tag="PB780_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDE0734034.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11219..12844
                     /gene="groL"
                     /locus_tag="PB780_001988"
     CDS             11219..12844
                     /gene="groL"
                     /locus_tag="PB780_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDE0734035.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4369 a         2265 c         2833 g         3679 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9961 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10021 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10081 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10141 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10201 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10261 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10321 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10381 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10441 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10501 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10561 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10621 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10681 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10741 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10801 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10861 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10921 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10981 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11041 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11101 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11161 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11221 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11281 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11341 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11401 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11461 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11521 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11581 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11641 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11701 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11761 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11821 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11881 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11941 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12001 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12061 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12121 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12181 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12241 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12301 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12361 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12421 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12481 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12541 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12601 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12661 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12721 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12781 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12841 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12901 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca
    12961 aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc tgtttatacg
    13021 tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt tttgtgtgta
    13081 attataatga aaatagttat ttttaatagt tataatattt tatctattaa aagtaaatat
    13141 ccattc
//
LOCUS       DAMEET010000074        12868 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.075, whole genome shotgun sequence.
ACCESSION   DAMEET010000074 DAMEET010000000
VERSION     DAMEET010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.075"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            127..3654
                     /locus_tag="PB780_001989"
     CDS             127..3654
                     /locus_tag="PB780_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HDE0734036.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="PB780_001990"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="PB780_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HDE0734037.1"
                     /translation="MNQIPLKPKNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="PB780_001991"
     CDS             7442..7783
                     /locus_tag="PB780_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDE0734038.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="PB780_001992"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="PB780_001992"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDE0734039.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /locus_tag="PB780_001993"
     CDS             9140..11560
                     /locus_tag="PB780_001993"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDE0734040.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="PB780_001994"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="PB780_001994"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDE0734041.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         4369 a         2239 c         2731 g         3529 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgaaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatgga
//
LOCUS       DAMEET010000075        12278 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.076, whole genome shotgun sequence.
ACCESSION   DAMEET010000075 DAMEET010000000
VERSION     DAMEET010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.076"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(180..737)
                     /gene="frr"
                     /locus_tag="PB780_001995"
     CDS             complement(180..737)
                     /gene="frr"
                     /locus_tag="PB780_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDE0734042.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(740..1462)
                     /locus_tag="PB780_001996"
     CDS             complement(740..1462)
                     /locus_tag="PB780_001996"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDE0734043.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1598..2479)
                     /locus_tag="PB780_001997"
     CDS             complement(1598..2479)
                     /locus_tag="PB780_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDE0734044.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2578..3360)
                     /gene="rpsB"
                     /locus_tag="PB780_001998"
     CDS             complement(2578..3360)
                     /gene="rpsB"
                     /locus_tag="PB780_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDE0734045.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3719..4198
                     /locus_tag="PB780_001999"
     CDS             3719..4198
                     /locus_tag="PB780_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HDE0734046.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4415..5506
                     /gene="phnW"
                     /locus_tag="PB780_002000"
     CDS             4415..5506
                     /gene="phnW"
                     /locus_tag="PB780_002000"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HDE0734047.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5499..5627
                     /locus_tag="PB780_002001"
     CDS             5499..5627
                     /locus_tag="PB780_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HDE0734048.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5631..6176
                     /locus_tag="PB780_002002"
     CDS             5631..6176
                     /locus_tag="PB780_002002"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HDE0734049.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6630..8321)
                     /locus_tag="PB780_002003"
     CDS             complement(6630..8321)
                     /locus_tag="PB780_002003"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDE0734050.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8741..9688)
                     /gene="arcC"
                     /locus_tag="PB780_002004"
     CDS             complement(8741..9688)
                     /gene="arcC"
                     /locus_tag="PB780_002004"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDE0734051.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9803..10822)
                     /gene="argF"
                     /locus_tag="PB780_002005"
     CDS             complement(9803..10822)
                     /gene="argF"
                     /locus_tag="PB780_002005"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDE0734052.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10913..12142)
                     /gene="arcA"
                     /locus_tag="PB780_002006"
     CDS             complement(10913..12142)
                     /gene="arcA"
                     /locus_tag="PB780_002006"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HDE0734053.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3370 a         2540 c         2209 g         4159 t
ORIGIN      
        1 acttaaaaga ttcccactat cttaagtatt atagcaatat ctaactgttt ttcctatgaa
       61 atcattgttt ttttatttaa aaactgaata attttttctt tttttaattt ttaaataaaa
      121 gaaatataaa taaaaacaga ggaagcggaa tgatccgccc ctctgttttt atgattttat
      181 taaacttcca ataattcttt ttctttatca gcagcgatct tatctacttc tttgatgctg
      241 ttatctgtca atgtttgtac gtctttttct aatccgcgca aatcatcttc ggtaatatca
      301 ccattttttt gttgtttttt atattcatcg atcgcatcac gacggatgtt acgaatcgcg
      361 atttttgcat tttctgcttc ttttttcaca tctttagcta attcttttct gcgttcttct
      421 gtcagttgcg ggataactaa gcgaatgaca tttccatcat tggttggact gattccgata
      481 tcacttgcca taatagcttt ttcaacatct tgaagaatgc tcttgtcaaa aggtgtgatc
      541 attaaaacac gtgcttctgg aatttggatt gatgccattt ggttcaatgg tgttggcgca
      601 ccgtagtagt tcacagtaat acggtccaaa aggctagcgt tcgcacggcc tgcgcggatt
      661 tgcccaagtt cacgttgaag attttgtgct gcttttttca ttttttcttt tgcttctgtc
      721 ataattgcat ctgccatttt tatttccccc ttacagttgt tccgatattt tcgcctaaga
      781 ttgcacggcg aatatttccg tgttcattta aattgaagac aagcagtgga atatcattat
      841 ccatgcttag tgaacttgca gttgaatcca tgacttgtaa tcctttggca ataacttcca
      901 gatgagtcag ttcttcaaat tttactgcat ttgcatctac tttcggatca gcagaataaa
      961 caccgtctac attgttttta gccatcagga taacatctgc gccgatttct gccgcgcgta
     1021 atgctgctgt tgtgtctgtc gagaagtacg ggttgccggt accgcctgca aagataacga
     1081 tccgtccttt ttctaaatga cgttctgctt tacggcgaat atatggctct gcgatttgac
     1141 gcatttcaat cgaagtttgc acgcgtgtcg gtacacccac gttttccaac gtatcctgta
     1201 aagctaaggc attcataact gtagctaaca tacccatgta atcagcttga gcacgttcca
     1261 tgcccatttg tgctccgatt tgtccgcgcc agatatttcc gccgcccaca acgattgcca
     1321 tttcaacgcc cagctcgtga acttctttga tttcttggat gatttctttg attactggtg
     1381 gtttgattcc aaatcctgcg tccccagcta aagcttctcc actcaatttt aagactacac
     1441 gctgatattt cggtgtgacc atgttcattc ctccaatatt ttctattgcc attttaccat
     1501 acttagatga tttcgacact ctaagactgg tttttcttgt tttgcttaaa aaaagggagc
     1561 gcacaaaccg tgcgttcccg tctgttattc aacagaatta ttttttcact tggctcataa
     1621 cttcttctac aaagttatct tcgcgttttt cgataccttc gccaacttcg aaacgtacga
     1681 atgttttaac agttgcgcct tttgatgcaa cgtatttttc aacagtcata tcaggatctt
     1741 taacgaatgg ttggtccacc aaagcgattt ctgctttgaa tttcttcaag cgtccttcta
     1801 ccattttttc aacgatattc gctggtttgc cttcgttcaa tgcttgttct gtaagaacag
     1861 ttttttcatg ttctaattca gcttctggaa tttgtgattc gtttacataa cgaggattga
     1921 tagctgctac gtgcatagct acgtctcttg caactgtttc gtcagttgtt ccatctagaa
     1981 cagtcaatac ggcaatacgt ccgcccatat gcaagtatcc accaaatgca gcattgtcgt
     2041 ctttttctac aacttcaaag cgacggaagc tgattttttc tccgataact tgagttgctt
     2101 cgatcaagtc agattcaatt gtacctttat ctgttttgat tttcattgca gcttccatgt
     2161 ctgctggttt gttttcagca acaagttctg caatttcttt tactaaatct tggaacattt
     2221 cgtttttaga aacaaagtct gtttctgagt ttacttcaac gattgctgca gtgttacctt
     2281 tcacagcaac tgaagccaat ccttcagctg caatgcggtc atttttcttc gcagcctttg
     2341 ccattccttt ttcacgtaaa agatcaactg ctttttccat gtcgccttct acttcaacta
     2401 atgctttttt cgcatccatc attcctacgc cagtcatttc gcgtagttct tttaccattt
     2461 tagctgtaac gtctgccatg tttaactcct cctgtgtgtt tttcttttta aaaaaagctg
     2521 cttcaaagga gagtgaggct tgtcgcccag cctttgaaac agctccatca ttcaaaatta
     2581 ttctgctgaa gaattgtcgc cttcaacaac atctacgatt tcttcgattg atgttgctgt
     2641 gttttcttct gtgaaatctt cttcaactac ttgatcttca ccttggtttc cttcgatgaa
     2701 agcatcagcc atttttgaag taatcaattt aacggcacga attgcatcgt cgtttgaagg
     2761 gattactacg tcgatttcat ctggatcgca gtttgtatca accatagcta cgattgggat
     2821 atttaatttg tgtgcttctt gaacagcaat acgttcttta cgtgggtcaa cgatgtacat
     2881 tacatctggg attcttggca tatcagcgat accgcctaag aatttttcaa gacgttcacg
     2941 ttctttgttc aaaccagcaa cttctttttt aggtagaact tcaaaagttc catcttcttc
     3001 cattgcattg atttgtttca aacggctgat acgtttttgg atagtatccc agtttgtcaa
     3061 tgttccgcct aaccaacgat ggttaacgaa gtattgtcct gcacgagttg cttcttcttt
     3121 gattgcttct tgtgcttgtt tttttgtacc tacgaataat gctacgccgc cttcttcagc
     3181 aacgctcttc atgtaatcat aagctgcatc tactaattta actgtttttt gcaagtcaat
     3241 gatgtagatt ccgtttcttt ctgtgaagat atatttcttc atttttggat tccagcgacg
     3301 agtttggtga ccaaagtgta cgccggcttc tagtaattgt ttcatagaaa ttactgccat
     3361 gtttgtttcc tccgatttgg ttttattttt cctcttctta tcttctacct tgccggcaaa
     3421 ctttttcaag cacctaccga caatccaata agaatgtgga tttgctgatg cataaagcac
     3481 cctcagctat tatacagaca aaagatccgt atcgcaagag caaatatact tttctgctca
     3541 acattcaata gtttacctga aagataaaaa gaattcaaga aaaacagtag atttctcttt
     3601 gtttctttat tatccttttt ccataaaata attttaggta taaaagacga atattattgg
     3661 gcaatccttc ttttttttgt tatgatacat gtgaaatctt tcgtaaagga gaaagaaaat
     3721 gcgcaaagct caaagacatt tgctgattaa acaagtcatt gaagaattcc cgatccgtac
     3781 gcaagaagaa ctattaaaca aacttgggca ttatggcgtt accgccacgc aggcaacgat
     3841 ttctcgagat atccgtgatc taaaaatcgt gaaggcacct gatgaaaatg gcgtttcccg
     3901 cttcgtattg tttcgcgaaa aaggaactcc tgagactagg actgaggaag aggatcggct
     3961 ggttcaaatg attgaagaca ttgtattgaa agtcgaacga gtccactttt tgacgatcgt
     4021 caataccttg ccagacaatg ctcatttatt tgcatctgtt ttagatgaga tcaaacctcc
     4081 ctctattgtc agtacaattg ctggattcga tacgatcatg gtcatctcgg aaaatgagga
     4141 ggacgcaaaa cgagtagaag aatttcttca ccatccaact gaagtatcac ttttttaatc
     4201 agagcacgta ttcctaatat ggaatgcgtg tttttttatt ttcaatattt tccttattga
     4261 ataaaccttt acactcttta caaaaataca tcatttttta ttcaaatttt tcaacacctt
     4321 gattgaatca caataatcat catttatatt ggatgttaac aagagcagct tcttgctctt
     4381 tgtgtatttc aaaaaagaaa tgaaaggaaa tcatatgtat aaattattaa cttctggacc
     4441 attgacgact tctgaaacag tgaaaaatgc tatgctgatc gatcattgta cttgggatca
     4501 agaatacaaa gaaattacac aatggataag aaaaaaatta ttggatcttg ctcaagtatc
     4561 ggaaggaata tacacaacgg tcttaatgca aggtagcagc tccttcgggg tagaatcggt
     4621 attaagttct gtgatcaggc cagaagatac tgtactgatt tgttcaaacg gcgcatatag
     4681 acaacgaatg atcaacatgt gcgaatgtct gaaactgaac tataccgttt atgaggtatc
     4741 ggaacatcaa atcctccaag ctcatgtgat tacacagctg attgaagaga atccgtctat
     4801 ctccgcacta gcaatggtcc atagcgagac tacttccggt attttgaata atttggaaga
     4861 gattgctgct gtcacacaag aaagagatct tttgtttata gtggacgcga tgtcaagctt
     4921 tggtgggata ccgatttgtg tagccgaact agggattgat tttttgattt ccagctcaaa
     4981 caaatgtatt caaggtgttc caggcttttc ttttgtactt gctaataaag cggaactgga
     5041 aaaacgaaaa aatgttagcc gaagtttgtc acttgatcta tatgaccagt accgcgtgat
     5101 ggaaaacgag aatggcaaat ggcgtttcac ttcccctacc catacggttt tggcttttca
     5161 acaagcgctg aaggaactag aggcagaagg aggaatcgaa actcgatatt cacgttatct
     5221 cgccaataat catgtgctac gacaaaaaat gacagagatg gggttccagc ctttcattca
     5281 agaatatcag gggccattca tcacctcttt tctttaccct aaggactggc gatttgattt
     5341 taatcatttt tatcagtatt taaagcaaaa tggatttgtg atctatcccg ggaaaatcag
     5401 cgatcatgaa tgttttcgta tcggaaatat tggcgagatc tatccggaag atattttgga
     5461 actgacagca attatcaaag actataagga gaacgttaat gattaaaact gtaatttttg
     5521 attgggcagg aacaactgtg gactttggct gtatggctcc tgtccacgct tttagaaatg
     5581 cttttctaga aaaaggaatc caactgactg acaaagaaat ccgctaaccg atgggaaaat
     5641 taaaatggga tcatatccaa cagcttcttt ccctcccttc tgtcaaaaaa caatggatag
     5701 ctatttatgg acaactacct caggaaaaag aggtggagga attgtatcaa caatttgaac
     5761 attatctgtt taaagatttg gtcaagcatt ctaccttgaa acccgacaca ttggaaacga
     5821 tcgaacggtt gagaaacgct gggatcaata ttggttccac gaatggattt aatgctccta
     5881 tgatgacaat cgtagcagaa accgcaaaaa aagaaaggta ttcaccagac ttcatggcaa
     5941 cttttgaaga cgtcgaaggg tatggtcgac cgtttcctta tatgatccat aaaaacatgc
     6001 agtacttcaa aaacaagtcg gttgatgaag taatcaaagt cggagatacg gtctcagata
     6061 tccaggaagg gaaaaatgcc ggagtgttga cagtaggtgt aattgaaggt agttcgctaa
     6121 tggggatttc ttatgatgag taccaaaaat tagagatggg gaaaaagatc gtttaaaaca
     6181 gtctgttgct cttagattcc aacaagccgg cgctgatatt tgtgtaaaca atttaaaaga
     6241 gttaacagaa tacattttgt acgctgacaa gaatccagaa tgacttttat acaaaataag
     6301 aggttgtgaa aaagctgtcc tgtatcaagt aataaaaaca gccaagcaaa aatagctcct
     6361 catatttttc gtttggttag gcttattacc gcagatgcaa gcttttgaac accgtttatt
     6421 cgaaaaaaaa aagggagctg tgacacgact tttgtcacag tccccttatt tgcaaacaaa
     6481 cagtgtttaa gagacaacct ctccactaaa cggtgttcat tcagctgact ctcggtattt
     6541 gctcaaaaaa cgataaaatt tgaaaagcta ttttctcttt tgtttctcta atactcggat
     6601 cagaacgctg atttcacatt ctctttttct tacatcttat ctggtgcatg aagtcctagc
     6661 aaacgaaggt cttctttcaa taatacagtc actgcataaa ctagagctaa gcgtgcttct
     6721 ttttgttcat catcagctaa aatcttcgta tgagcatagt atttgttgaa tgcttgtgct
     6781 aatttgattg catgtttcgc aatcactgac ggttcgtatt tttccccagc agacaagacc
     6841 gtttcagggt atttttgaac taatttaacc acttcccagc tggtatcgtc attcaatgca
     6901 tagtctgctg tttctgaagg gacaaatcct gctttttcca acaggctgac tgcacgagca
     6961 tgtgtatatt gcacataagg tccagtttct ccttcaaatc gaacaacttc ttccaggtta
     7021 aagtcaaatg tattcagtcg atcgtttttc aaatcgtgga aaatgactgc cccaacccca
     7081 acttgtttag ctacagcatc tttgttttcc aaatcagggt ttttctcgct gatttgttct
     7141 ttagcagatt ggattgcttc atttaatact tcttctaata agacgatctt tcctttacga
     7201 gttgataatt ttttaccacc ttgcgtgatc aaaccaaatg gaatatggtg catgtcgtca
     7261 gaccagtcaa agcccatttc cttcaagaca gctttcaact gtttaaaatg atagctttgt
     7321 tcattcccta ctacatatag agattgtttg aagtcgtaag tacgtttacg gtacaatgca
     7381 gcagctaagt cacgtgtaat gtatagtgtc gcaccgtctg acttcttaat caacgctgga
     7441 ttcaggtcat aagcagatag gtcaacgatc tctgcacctt tatcctcgtt cagcaaatgt
     7501 ttttcttcta aaagtttgac gatttcatcc atcttgtcat tgtagaaagc ttcaccgttc
     7561 aacgaatcga aacgtacttc cagcagatca tagattttat tgaattcttt catcgattca
     7621 tcacggaacc attgccataa ttgaatagcc tctttatcgc cttcttccag acgtttgaac
     7681 caagcacgag cttcttcatt taattctggt tctgtttcag ctacttcatg gaattggacg
     7741 tataaacgaa gcaattcatt gattggttct gctttaacag cttcttctgt accccatttt
     7801 ttgtaagcga cgatcagttt tccaaactgt gttccccagt cacctaaatg gttgatgcgg
     7861 attggctgat aaccgatttt ttccataatg aaaccgatcg aattaccgat aactgtcgaa
     7921 cgcaaatgtc ccattgagat tggtttggca atattaggag aagacatgtc gattggtact
     7981 gttccttgat tcccgatgtt gctatcccca taatgttctt tttctttcac tacggtttgc
     8041 aaaacttttt tgctgataag ttctttattc atgaaaaagt tcaagtaagg tccgacaact
     8101 tcgatttttt caaagttggc agaatcgatt ttttctgcta aatctgcagc gatttgctgt
     8161 ggcgcttttc ggtaaacttt tgctaatgaa aaggctggaa atgcaacatc tccgtgttca
     8221 gctgattttg gattttctag taattgctcg acttgttcca gtgttaaatc atctttgaca
     8281 gcgtcataga cagcttttgc gacaagatct ttattattca tctttattcc tcctaaagtg
     8341 ttcccttgat tgttaaacaa aaaaaatctc tagcgacggc atacttagta tgccatcact
     8401 agagacgaga tttcccgcgg taccactcca attgtcctaa aaaaggacca gctccatctg
     8461 atatcggtag acgaccgtct gtttaaaaca gaattctcca agtgcgcttc gttgtatctt
     8521 cttcattcgg cttccaccac ttccgaattc gctagccaag aatgatacaa gtactctctt
     8581 gttcaacaaa tcttagtctt attaaacttt cttgaattat atcagaaaaa aaaacgaaag
     8641 gctagtcgtt tcataaaaaa aaaatccaga tggcctaaag gagaagaaat accacctgga
     8701 tagctataaa tcatttcgta aatacgaaag acttaacaaa ttaatcagct acgataatcg
     8761 ttccttcttc agaagctaaa agattctcga tattttctaa agaagtgatg attgccttag
     8821 cgtttggttt tgcttcaaca aatgcaatag ctgcttctac tttcggaagc atgcttcctg
     8881 gagcgaattg cttttcatca atgtattgtt tcatttctga aacagtaaca gtttctaatt
     8941 tcttttgatc tggtttttgg taattgacat acacattgtc tactcctgtc aatacgatca
     9001 gcaaatccgc atcgatgatt tcggctaatt tttctgaagc aaaatcttta tcgattacgg
     9061 cttctcgtcc ttctaatcca gtagctgttt ctacaacagg aatcccaccg ccaccaacag
     9121 agactgtgat aactccttga tcgaccagcg tttcgatgac acgtgcttct ttaatcgaga
     9181 tgggtttagg ggaagcgact acttttctcc atccacgtcc agcatcttct ttgaatgtca
     9241 cagtggagtc agcattcatt tgttctttgg cttcttcttc cgtgtagaat ggaccgactg
     9301 gtttacttgg atttttgaat gaaggatcat tttcatccac aataacttga gtgactaaag
     9361 aaaccacatc tttatcgatc cctttttcct tcagtacttc gcccatggcg ttttgtaacc
     9421 agtaaccgat agatccttct gtcattgcaa cacatgtatc taaaggcatt gccggtgttt
     9481 tctcagaatc agcagcttgc tgctgaatca ataggttacc gacttgcgga ccatttccgt
     9541 gggagatgat caattcatct ccttgttcga taaatttcac taaatacttt gctgtttcca
     9601 ttaacgcttc ttgttgggct tttgcacttg catccgtaga caagatggca ttgcccccta
     9661 aagcgacaac tacttttcgt ttggccattt cattcgtcct ttcttcagtt ctaccgaatt
     9721 ttctagataa caaaacgagc cagcgttttg tgattttccc tcaatgctga ctcgtttttt
     9781 cgtgagatga atgtggttat gcttaaacgc gagggataaa caagttacct aatgttgctg
     9841 ccatgatcgc tttgattgag tgcatacgat tttctgcttg gtcaaattga cgagcatatt
     9901 tgctgcggaa gacttcatct gttacttcca tttcagtgat gccaaagcgt tctttcattt
     9961 gttcaccgta aacagtgttt gtatcatgga aagctggcaa gcagtgcaag aagatcaaac
    10021 gatctgtgtt gtgtgttttt tctaccattt ccatattgat ttggtaaggt ttcaacaatt
    10081 tgatacgttc ttcgaattta tcttcttcac ccattgatac ccatacatca gaataaagga
    10141 cgtctgctcc gtctacacct ttatcaacat cgtcggtaat catcaattgt gcaccggatt
    10201 tttcagcaaa gccttctgcc attttgacga tttcttcttc aggttgtaat tcttttggtg
    10261 caacgatacg catattcgct cctaaaattg cacctgtgac taataaagag ttagccatat
    10321 tgtttcgacc atctccgcag taagcaacag tgataccttc tactgttcca aagttttctt
    10381 ggatagttaa aaagtcagcg atcatttgtg ttggatgcca ttcatcagtc aagccattcc
    10441 aaactggaac accagagaat tctgctagtt cctctaccat tttttgactg aatccacgga
    10501 attcgattcc gtcaaacata cgtcctagta ctttagctgt atcttctgtg gattcttttt
    10561 tacctaattg gatatcattt gcacctagat attcaggatg tgcacctaga tcgatcgctg
    10621 cagtagtaaa tgcagaacga gtacgtgtag atgttttttc aaataaaagc gcaatatttt
    10681 ttccttctag atagtgatgt gggataccac gttttttcaa gtctttcaag tgttctgaaa
    10741 aatcgatcaa atattgtaat tcttcttttg taaagtcttt ttctgctaat aagcttcttc
    10801 cttggaatac tgattctttc atattaaaca ttctccttta gattgttttt atttaagatt
    10861 aaagaggaaa tcccgacggc agctgacagt aagactgccg tcaggaaaca aattacttca
    10921 agtcttcacg gacaagtggt tggctcatgc agcgtggacc accacgacct cgtgacaatt
    10981 cacttgaatt gatttctaac actttgatgc catagctacg aagcaattcg ttcgatacat
    11041 aatttcggtt ataagtgact acaacacctg gagcgattgc caatgtgttt gatccatcat
    11101 tccattgttc tcgaggtgca acgatctcat ctccattacc agttgggatc agtacaagat
    11161 catctaatcc aagagcagct ttcatcgttt cgtgtaagtt atctgaatga gtaatcttga
    11221 tatcatcacc atctggttcg attgtaaaga catccatctt accgtcttta ctttgaattg
    11281 ctggatggat agtgaattta tcgtgatcta ccattgtaaa tacggtatcc aaatgcatca
    11341 ttgcacggtt gttcggaatt ttgattgcca acactttttc aaatcctgaa ttttttgcga
    11401 ataggtggcg tgctaatttt tctaaagctt tcgcattagt tcgttgagaa atcccaacag
    11461 ctactacttt atcgcttaag atcaattcgt ctccgccttc gatatgatct ggattttcac
    11521 gatccaacca tacttcgatt cctttatttg caaatcttgg atgatgtttc aaaatatatt
    11581 ctgtaaacat cgattcacga cgacgtgctt caaaagtcat cttattgact gtcatcccat
    11641 ttcccattga tgcggaaggg tcgcgtgtaa agtacaagtt tggcattggg tccatgtaaa
    11701 atggatagtc atcgtctgct gacagatcat atagactgct agaacgtact tcgatatctt
    11761 ttgtacgaac cccagccatg attttatcta ccatttcttg cgtttccatt gatagaagga
    11821 actcatgaag accctctctt acagcattcg atgcaatatg agattcatcc aacattttat
    11881 tcaagaattg ttctttgaca ttgcctgcgt caatcgcttc tgccgctaat ttttccaagt
    11941 aaagagtctc gacgccttca ttttgcaatg tcttcgcaaa gttgtcatgt tcttcttgag
    12001 caatcggaag atatggaata tcatcgaaca acagacgatc cataatatct ggtgtcaaat
    12061 tttccacttc ttgtccaggc cgtttcagta aaactgtctt taatttccct atttcagaga
    12121 aaacgtgaat aggtttatcc atgttgtgtt gcctccttct ttttgattac atgtatagga
    12181 tatcctcctt ttgtaatcac tttcataatt ttgtctcatt caaaatgaga cattcctcac
    12241 attaatattc gataattaaa taattaaacg tttttcta
//
LOCUS       DAMEET010000076        11925 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.077, whole genome shotgun sequence.
ACCESSION   DAMEET010000076 DAMEET010000000
VERSION     DAMEET010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.077"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            559..1530
                     /locus_tag="PB780_002007"
     CDS             559..1530
                     /locus_tag="PB780_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDE0734054.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            complement(1608..1844)
                     /locus_tag="PB780_002008"
     CDS             complement(1608..1844)
                     /locus_tag="PB780_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDE0734055.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(1813..1944)
                     /locus_tag="PB780_002009"
     CDS             complement(1813..1944)
                     /locus_tag="PB780_002009"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDE0734056.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            complement(2020..2403)
                     /locus_tag="PB780_002010"
     CDS             complement(2020..2403)
                     /locus_tag="PB780_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDE0734057.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            complement(2436..3221)
                     /locus_tag="PB780_002011"
     CDS             complement(2436..3221)
                     /locus_tag="PB780_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HDE0734058.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            3399..4499
                     /locus_tag="PB780_002012"
     CDS             3399..4499
                     /locus_tag="PB780_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDE0734059.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            4489..5313
                     /locus_tag="PB780_002013"
     CDS             4489..5313
                     /locus_tag="PB780_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HDE0734060.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            5310..6125
                     /locus_tag="PB780_002014"
     CDS             5310..6125
                     /locus_tag="PB780_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDE0734061.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            6122..6952
                     /locus_tag="PB780_002015"
     CDS             6122..6952
                     /locus_tag="PB780_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDE0734062.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            6959..7990
                     /locus_tag="PB780_002016"
     CDS             6959..7990
                     /locus_tag="PB780_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDE0734063.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(8097..9370)
                     /locus_tag="PB780_002017"
                     /pseudo
     CDS             complement(8097..9370)
                     /locus_tag="PB780_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            9597..9782
                     /locus_tag="PB780_002018"
     CDS             9597..9782
                     /locus_tag="PB780_002018"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734064.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            9906..10622
                     /locus_tag="PB780_002019"
     CDS             9906..10622
                     /locus_tag="PB780_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDE0734065.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(10524..11044)
                     /locus_tag="PB780_002020"
                     /pseudo
     CDS             complement(10524..11044)
                     /locus_tag="PB780_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            11332..>11925
                     /locus_tag="PB780_002021"
     CDS             11332..>11925
                     /locus_tag="PB780_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDE0734066.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKK"
BASE COUNT         3433 a         2200 c         2721 g         3571 t
ORIGIN      
        1 taacgtgatt ttttgctgta atgacaaggt tttagctgtt tcagatacca tgtatccaaa
       61 aatagtttat tgagaaaata ggagagtatt tgatactaac tagtaggaaa aaatgagata
      121 aaaagtaaat ggaatcattt aaggtaatct gaaataaaaa atatacagcc ctgtggtact
      181 ttacctacaa ggctgtatat attttttact ctcagcgttt ttgactccgt gtctgactcc
      241 gttttattga ttttgaatgt tattcaatga ccatttaaca ggtcaaattt gatgtttttg
      301 attccgtatg acgccgtttg atatctagtg atatagtgat ttgagtctac atcgaataag
      361 ggaataaacc aataaaaaat gatttagaat attaaaataa caaaaaagag gactacctgt
      421 attagtcctc ttttttgacc agattttgac cagtctattt tacatcctag tatatttata
      481 tgagctaatt ttatgattaa atggtaagtt tttccttgtg tgttttagtt taaactagtg
      541 tgtaattcaa aaatctatat ggaaggaaca gtattaagaa tagaacaggg ttctctacat
      601 gatggtgctg gtttacggac cgttgtttat ttaaagggct gcccattacg ctgtgcctgg
      661 tgttcgattc ccgaatcaca aagcaagcag attgaaaaag gatttggcca gaccatgacc
      721 gctgaagagg taatggatga gattgagaag gatgctgttt tttactttca ctcggatggc
      781 ggagtgacca ttagcggagg cgaggcactg gttcaggcgg attttgctaa agaaattttg
      841 caaaaatcga aatacattgg aatcaatacc gtactggaaa ccagcttttg tggtgcttat
      901 aatgaaatcc aaaaggtagc tccctatgtg gatacattgt ttgtggatgt aaaaatgttt
      961 accagcaagc tccacaaaca atggaccggc ttagataacc aacaaatttt gaagaatatt
     1021 cgtcgctttt taattgagta tcctaactgc gaagtgcgaa ttcgagttcc tgtggttcca
     1081 ggtatcaata tgaacctgac cgagctgctg acgattgctt gttttgtggc agatcttgat
     1141 cggttcgtac cgttagaatt actaccgtac cactgctatg gtatgcacgg ttaccaagca
     1201 ttgggactgg agtatccgtt ggcagacacg ccagcgcctt cacctaaaga aatgtttgca
     1261 ttggcagatc aactggctcg cacggttcct catttgctgg tcacagcttt aaccaaaaca
     1321 tttatttatt tattgaatcc aagctttatc ttatggataa atgaaagaca ctcactagct
     1381 gcagccagct caagaataga gcttttgctg aagtcagtga gtgtcttttt acgatgtttt
     1441 ttcgaagtta atttgtcgaa tgaacactgg ctcaatcggt acatcttcac tgtttgtcgg
     1501 ctgagctgcg atcgctgcta cgatttctaa cccttggtat acctgtccaa aaacagtata
     1561 acgaccatcc agctccggta gtcctccaag ctccaagtat gcttgcttta cattagcagg
     1621 tcagcctgcg cctttgtgtt ttgcacaata aaaaactggt tgccgttggt gtgggggccg
     1681 tggtccccta aggccaaggc gccattaaaa tgataaagtc cgcgatgcac ttcatcagcg
     1741 aaaaagccct catagctgga aacagcttca tcaccagaac catccaaagc gccaccttga
     1801 ataacaaatt ccttaatgac acgagccgaa aggtgtgttg tcataaacgc cttgctcagc
     1861 tagggagcgc cagttggcta cagccagggg agcttggtca ggaaataagc ggagacgaat
     1921 ttcaccagca tcggtgacca gcacacaaac ttcttcctgc tgcggttttt ctaattgaaa
     1981 gagaggaagg tttgcaactg aaacgccttg atcctgccat caaatatcga aaatccgcca
     2041 ctcgaccttc gcctgatcac caaggcgagc cattcccaat aaaacggtat aggcggtttc
     2101 atccgcttga aaggtcaatt gcagctgtcc tcgtaaatcg ttgtagtcat ctggggcggc
     2161 tacttgataa aaaagatgaa actgcgcatg caaatattcc aaaggctgat ctttaaaggc
     2221 tgcaaaataa gcctgaactt tttcccgctg caagctgtca gccaccaaaa aattttccag
     2281 ttgttgtttt ggaacggctg ctaacaagga tcggcaaacc gcctcaattt tcaacgcatc
     2341 ccaatgtcgc gcaattaaat aatggatggg tgcctcttga ttgtagtccc acaggttgct
     2401 catttttttc accactttct tctattatat aggaatcaca aaatcggcag cggcgtcagg
     2461 cggcgttctt ttccttgaat gactagcaac ttttctacct gacagtctgc caacagctga
     2521 ctcatttttt taaagcccag ctgataatct tcggcaacat gagcatccga gcctaaggta
     2581 aagagctttc cgccgacact ttgatacaaa ggaacggcat aacggtaaag gtcttcgttg
     2641 ccgtatttca agaagctctt ggcatttaac tccatcgcca attctcgttc aacaaccttt
     2701 ttgaaaatcg ttagcagctg agcctcaaaa tgctccgcga attcttccgc tgtgaaggtg
     2761 aagcggcgta atccataatc aaagtgagtc aaaatatctc cttctggaaa atccgtcaag
     2821 acctgcaaca tctgatcaaa atattccttg gtagtttgaa tgggatcttt ggttaaaacc
     2881 acatcgtcca tataatcaaa gcggccattt tgatgaatac ttaccagctt taaatcataa
     2941 ggatgctgag cgaggtaatc cacgatccgc tcttcttgtc ctggaacaac gccaatctcg
     3001 atacctcgca gaaatttggt ggaataggtt tcttttaaag agtccagttt gtttatatag
     3061 gctggataat ccggaatgtc atcttttccg gtagatggat tgtttaaatc aaagtgatcc
     3121 gtagaaacga aaaactccgg ctgataggct aaatagtttt caaatttttc ctctgaatca
     3181 aaggaaaaat acgtgtggag atgctgatca taatactgca tagggagcct ccttttttct
     3241 actatcatac cgcaaaagct ttagaaggtt aacgggaatt cgtcgaaatt tacaaaccat
     3301 atacaacctt tacattgttc atgaaacctt ttcaagaaac tgattctctc attacaaagc
     3361 tttgttagaa tggaattatc gaaaggggag aagaatgaat gaaaatgaat gaaaaaattc
     3421 gtaacatgac tatcggttta ttttgtgcag caacagtaag tatcctgctg acaggctgcg
     3481 gctcaggtga agcaacgaag caatcggctg cggcagacga ctctttagat acgattatag
     3541 aaaaagccaa ggaagaagga gagattgcca gcttgggaat gccggatact tgggcaaatt
     3601 gggtaggcac ttggaacgac cttgaaaaag aatacggctt aagtcacgtg gatacggata
     3661 tgtccagtgc agaagaattg gctaagtttg aatcagaagg aaaaaatgga accgccgata
     3721 ttggtgatgt cggtatcagc ttcggtccgt tggctgaaag caaagagtta accttacctt
     3781 ataagaccaa ctattgggag gaaattccgg attgggcaaa ggatgatgat ggggattggt
     3841 tgttaagcta tacgggaacc atttccttta tcaccgataa agagaatgtg aagcaagcac
     3901 cgactagctg gaaggagctg caagagggtg actatcaagt tagcgtgggg gatgtgacga
     3961 ttgccaacca agcacaattt gctgtattgg ctgcggcgat tgccaacgga ggtgacgaaa
     4021 aggatattca gccaggtctg aattactaca aaaaaatggc agaaaaaggc cgtttgtcca
     4081 ccgttgatgc cagcgtagca aatcttgaaa aaggtgaaat cgatgtggca gttgtttggg
     4141 acttcaatgg actaaattat cgtgaccaaa ttgactccga gcgttttgag gtaactgtgc
     4201 caacagacgg ctccatcatt agcgggtata caaccttaat caataaaaat gcccctcatc
     4261 ccaatgcggc aaagctagca cgggaatata ttttatctga tgaagggcaa atcaatttgg
     4321 caaagggcta tgcgcgacct attcgcaagg tagagctgcc taaagaagta gctgaaaaat
     4381 tactgccgga ggatgcttat gaagcggcgg tgccagttga agacaatgaa gcttggaaca
     4441 agacctctgt agagcttcca gagctttggc aggcggaggt actgacctat gcaaagtaga
     4501 cttgacaaac tgattgtagt tatactagac ggctgtcgct acgacacagc cgtcgcccag
     4561 cttgggttca tgaatcattt agttgagcac cagcaagcaa gcttattgaa ggtaaaaagt
     4621 gagctgccca gcaattcacg tccgttatac gaagtactga tgaccggcgt tccagcctat
     4681 gaaaatggca tctatagtaa ttaccatgct cagcgatcca aagagtggtc gctgtttgaa
     4741 ttagttaagg aagctggcgg aaaaacagga gccgccgctt accactggta cagcgagttg
     4801 tacaatcaag ccccctataa tcgtttggtt gatcgcatcc agcatcagga ggagcgactg
     4861 attcagcatg gcatctttta tagtgaggac acttaccctg attcgcattt atttgcggat
     4921 gcccattatt tagtgaatca gtatcagcca gactttttgg tcgtacattc gatgaatatt
     4981 gatgatgtcg gtcacaaatt taccgccgat tctcatgaat atgcagcagc ggttaatcag
     5041 gcggacagat tattggctat ttaccttcct gaatggctgc aaaaaggtta tcaagtcgtg
     5101 gtgactgcgg atcacgggat ggatgcgcac ggactgcatg gcggcagctt ggctgcacat
     5161 cgtgaagttc ctttttattt gatttcaaat aaacgccaaa agctgaagga acaaactgta
     5221 ccacagctgc tggcggctcc attgttttgc tggttattag gaattgcacc gtcagagaaa
     5281 atgcaggaca ttcaagaatt gatgagggga tgaaagagat gaaaaaacgc ttggttctgg
     5341 ttcctgcctc ctttatcttc tgtgcatttt tactgctgcc gctgcttttt atggttctgt
     5401 gcagcttgca cagtgattta actaaccagt ggacattggc caattacagt gatattttca
     5461 gcaacaaata ttacacacag gcctttgtta acagcgtact gattgctgtc agctccagtg
     5521 tgattgggtt aatcgggacg tttattttat gtttatgctt aatgaatcta tcgactaaat
     5581 tgcaggagaa aatcaccttt gtttccaatt tggcagctaa ttttgctggg attccgctgg
     5641 cattctcttt tattattttg ttggggaatg taggtgtgct gaaaatgatt ttgccatttc
     5701 tagcggactt caatttgtat tcttggtggg gattgaccat cacgtatacc tatttccaaa
     5761 ttccgttagg ggtcttgttt ttgtatccca gcatgaagga agtgaaaaag gaatggctgg
     5821 agtccgcaca attgctgggc gcttcatcag cgtatgcttg gttcaaggtg gctttgcctt
     5881 ttttgcgacc atcgattgcc agcacctttg tcatcttatt tgctaatggc atggggacct
     5941 atgaaacagc gtatgccctg accagcagta atgtaaatct tttaacgatt cgaattgcgg
     6001 ctttagtgtc gggagatgtg ttcgccaagc ctaatttagg cagtgcgttg gcagtcgctt
     6061 taggtgtcct gctggtaacg gcaatgttga ttattcaaaa gaaaggcaag gtggtgcaga
     6121 cgtgaaaaag ttaataaaac cgattttgtt ctgctttgtc ctgtattact tgctgccgat
     6181 tattggaacg attttgtatg ccagctccac taaatggtca aaatcgcttt tacctagtga
     6241 ttttaccttg caatggttcc aacagctgct gacggacagg gaatttattg ctgcggtggg
     6301 tcgctcactt ttattagcag gtgtggtctt ggtgacgatt ctgttgttga tgattcccac
     6361 cattatttgg attcatttgt atttccctcg tttgaatcgt tggctggaaa agctgctcct
     6421 gctgccgtat gctttaccag gagtgatttt ggtaacggct ttgcttcgaa cgtatgctga
     6481 aactggtatc ccaatgtttg tagtgttggt aggtgcattg tttattacgg cgttgccgat
     6541 tgtctatctt agtcttaaca accagatgcg cttaattaat ctgaaggaat tagtggatgc
     6601 agcagaaacc ttgggcgccc cgatgtctac gattattatc caggtgctgt ttccaaacat
     6661 ccgtatcggt gtgaccctag tttctctgat gattttttct tcggtttttg gggagtacat
     6721 gctgacgaac ctgttgattg gcggtcgatt tcagacggta cgcatctata tgctgcggcg
     6781 gatgaacgaa aacggccatt tggctagcgc tgtgatggtt ttgtatttgc tgtttatggt
     6841 gggaattgct ttaactacct ttatgctgac ttatcgacaa aagcaacagc tgtcccttaa
     6901 gcagacgagg gaagagaagc agtcgaaaaa gaagccctta aaggagttgt gacggaaaat
     6961 gtttttagaa atacatgatc tcaatctgtt ttttgatgaa aaacagattt tagctattga
     7021 tgaaattcag attgaaaagg gtgagctggt tactttacta ggtcccagcg ggtgcggaaa
     7081 gtctacgttg ctgcgctgta tcacgggatt ggaacaacca aagagcggtg agattgtgct
     7141 ggacaatgag aatattgccg ataagcctac gaaggatcgc aatatcggct ttgtttttca
     7201 acagtatgca ttgtttccaa ctatgactgt ttttgaaaat gtagcctttg gtttgaaggt
     7261 aaagaagctg agtcctgagg tgattcagga aaaggttttt gagatgctat cattggtgga
     7321 tatgacggag caggcagata aaaatgtcca gtttctttct ggcggtcaac gacaacgggt
     7381 ggcactggct cgctcattgg tcactgaacc gaaagtcctg ctgctggatg agccattaag
     7441 tgctttggat gcccgaatta gaaaacagct gcagcgggat ttgcgcgcta tccagcaatc
     7501 gctagggatg acgatgattt ttgtgactca tgaccaagaa gaggcgatgc gaatcagtga
     7561 tcggattttt gtgatggaag ctgggcgggt ggctcaggtt tccacctcga aggaattgta
     7621 tcagaatcct cagagccgat ttgttgcgga atttatcggc aactataatc gtttcgaatg
     7681 gtatgagctg aatcagtata cccacgagtt ttccgctaag ggcaatcgct gcatttatta
     7741 tttaagaccg gagctgattc aatttgaacc ggttcctcag ggggttaaga ttccggttct
     7801 atggaaggaa tcatttattt taggcaacat tcaacggtat gtttttcaaa cgctggaggg
     7861 caaagaaatt ttagtggatc ggttgaacga tcgggaagag aaaattagcg atgccttata
     7921 cattcagctg aaagatattc tagcaatccc tgtggaaatt gatactcagc attttgcaca
     7981 acaaagctag tgtatagtcg gcaaacattt ctggctcttt gtcaaatagg actgatagag
     8041 tgatataaga acatctaagg tagaattgat tttctgcttt agatgttttt ttatgtttaa
     8101 taagtgatca gatgattgat taagaaaatt ctggttctca tcctaacccg taagctattc
     8161 gtttcaatgc tttgatgtgg gtatgcagat tttctatttt gccatttgag taaggcaact
     8221 ctaaggctaa acgaatgccg gattcatagt ttataaacgc tttttttata tgatgcagtt
     8281 ctcttggaac cgacttgggc attgttttta caagctgaaa gaagatgtcc gcttctttgt
     8341 cataaaaagc ttgaagaatt tcttgatagt aagcataagc tattcgtagt tcatcagaag
     8401 tagaaagtag acgttctacg acttcacttt cggttaaata ggcccaacca aaattacgcc
     8461 attttgtcca tttatcataa acaagcgtgt cctgtcgttt gatcagaagt cgagataaag
     8521 ccttcacttg tcgatagtgt ttggcttgta ttggatcgtt tcggtcgaag cttttcatga
     8581 ctcatagtca aatatcctta aaactgcgaa tgaggtgctt gacgatgtga aagcgatcca
     8641 caacgagttt ggcatgagga aagcactctt ttatcagcaa agcataagag gcgttcatat
     8701 ctgttacgag tatttttacg tttaaacgtg cacgtcgttc aaattgcata aaataagaaa
     8761 tgagatcttt cttcttacgt gaaggaagaa tatccagtaa tttcccagta tcgccatcca
     8821 tacaagaaaa agaataggga ccgacttgat ttttcaatga acgaaattca tctaccaatg
     8881 aacagcgagg caaccaattt attttcacgt ttgtcatggg gagcagagaa gcgagtgttc
     8941 gcgaaacggt tgaccaagaa acgccgcaat gtttagaaat cgtcgtcatt gcttgttttt
     9001 cagtcaattc ctccaatatt ttataatgta cttgtttggc gataaaacag tttttttcaa
     9061 caatgtaggt attcgccgta aaataggtgt gacagccacg acagaggtac cgttgctttg
     9121 ccaaagcaag ataagtccgt tgattcgcac acttattgag aaggaccagc gattttttct
     9181 ttccgttctt aacgatcagc ccatgttgat tcacctgttt acactgagga cagggctgaa
     9241 tcgttttaat gagcgtggca gaaacaacga tactgttgat tgaatgtatt tttttcttct
     9301 cgacatgagt gatttgaatg tttccatctt tgattcctag taatagtttg ataagatgaa
     9361 gatcaggcat acagaacgct cctttgattg tgttgtggtg attcaattct aacggagctt
     9421 ctgtatgttt tgttttttta tgctcaaaag gactgataga atgatttact atcagtccta
     9481 gaaattatag cgccacattt ctttcacctt tgacttttca gacggcagtt tttatgctga
     9541 tactgtagag agagcaggac acagcttttg cttgttttgg gtaaggggga aagaagatga
     9601 ccaaagcact tatttttgat tttgatggga cgatgattga cagtgaatgg atgttttacg
     9661 agagtattca tcactattta ctgaagccta tttactggag cagttcggct ataaaattcc
     9721 ttttgcagac tatcaacaag gtgttgggac ggcagcagaa ctgtcctttt atgaagggat
     9781 aagtcaagcg gttgggagac cagtggatgc tgaacatttt gagcagcgga tcagcagcac
     9841 tggaaaatcg gagtagtctc aattctgaac tgcgggagct agactatttt tttgaagcac
     9901 atccgatgat taaggaaaaa attgattcct ttgtgacgat cgatgatctt caggtcggca
     9961 aaccagatcc agaaggttat ttgttgtgct tgaaacggct gagggttgaa caggctgttg
    10021 tagttgagga tagtccagtc ggtgcccaag cagcaccgca tatgacagtt gatgtacaag
    10081 cgttggatgc agattttttc gcctttagcg ggcataaaat gtgcgggccg acaggaatcg
    10141 gtgtattata tggcaaacgt caattgttag aacaaatgga gccagtcgaa ttcggcggtg
    10201 aaatgataga ttttgtcaat ctttacgata gttcatggaa agagctgcca tggaaatttg
    10261 aagctggtac tccgaacatt gcaggagcaa tcgattactt gaatgagatt ggtatggaga
    10321 atattcatcg ttatgagcaa gaactggttg cctacgtttt acctaaatta caagcgatcg
    10381 gcggtatcac catttacgga ccgcaagatc ctgaacatca tactggcgtg gttgctttta
    10441 atttggatgg catccatccc cgtgatgtgg cgactgctct ggatatggaa ggtgtcgctg
    10501 tacgttcagg acaccattgt gcacaacggt tcatcgacga tttctgtttg actaattggt
    10561 cgtttaaaca gtcgatgata atgccgtttg agcggctcca atgtcgtgga atctttgagt
    10621 aagagtttcc aatatcgctt gagccgacgg tacttcttct gattttcaga ttacgtgatt
    10681 gattttcgta cagttctatg agaatcgcct gcttcagttc ctttgttagt tgatgatgtt
    10741 catcaactaa gtccgttttc gcagaaaagg tctgcccaca atcatagcat agaaaacggg
    10801 tacgttttaa ttctaaaata gtcaactttt ctttgacttt caacatctga gtacgtgtcg
    10861 tataggaacc gttgcggata atacgaatcg atccacagtg ttcacaatgg gaaggtgagt
    10921 catccaatct ccctttaata aactgcgctt gaacgccttt atattctttt tcaatcagcc
    10981 attcttcatc aaaaaataaa tgtttatctg ttaatccgag taattttcta gtatgattat
    11041 ccatagagcg actcctttgg tagggtgatg ttttggtcga ctaaatttta ccaaaagtga
    11101 gtctcttttt gagttgaaac gaagaaaaag tgttagtaga aaatcgcctt cgattttcca
    11161 acaacacaaa atattataga agcctttttt ctaaaggatt tcaaggtgtc tgtttgcatt
    11221 tattaaaaaa aagaaaaagt attggcaaca gaaggcgtca ccaacactag atattataga
    11281 gccataatat gctgtataat atctagtgta taggcttaag tggaggtata tatgaaaata
    11341 ttaactgttg aagatgataa tatgataaga gagggaataa gtgaatatct ttcagaattt
    11401 ggatatactg ttattcaagc taaagatgga agagaagcct tgtcaaaatt taatagcgat
    11461 ataaatttgg ttatcttaga cattcagata ccttttataa atggtttaga agtgttgaaa
    11521 gaaattagaa agaaaagcaa tttaccaatt ctaatcttga ctgcatttag tgatgaagaa
    11581 tataaaattg atgcatttac taatttagta gacggatatg tagaaaagcc attttcattg
    11641 ccggtcttaa aagctagaat agattcttta attaagaaga attatggaca tttagagaag
    11701 tttgaatata aggatctatc ggttaatttt aatagctata cagctaaaat aaatggtgaa
    11761 gaaatagatg taaatgcaaa agaactagaa gtattaaaat gtttattgga taataatgga
    11821 caagtgttaa caagaatgca aattattgat tatgtatgga aagatagcga agaaactcct
    11881 tatgatcgag taatagatgt atatataaaa gaacttagaa aaaaa
//
LOCUS       DAMEET010000077        11626 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.078, whole genome shotgun sequence.
ACCESSION   DAMEET010000077 DAMEET010000000
VERSION     DAMEET010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.078"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..646
                     /locus_tag="PB780_002022"
     CDS             <1..646
                     /locus_tag="PB780_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HDE0734067.1"
                     /translation="SSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFGE
                     DLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVYP
                     KLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEIK
                     EKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(755..1906)
                     /locus_tag="PB780_002023"
     CDS             complement(755..1906)
                     /locus_tag="PB780_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDE0734068.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(2060..2590)
                     /locus_tag="PB780_002024"
     CDS             complement(2060..2590)
                     /locus_tag="PB780_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDE0734069.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(2689..3039)
                     /locus_tag="PB780_002025"
     CDS             complement(2689..3039)
                     /locus_tag="PB780_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HDE0734070.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(3196..4725)
                     /gene="ahpF"
                     /locus_tag="PB780_002026"
     CDS             complement(3196..4725)
                     /gene="ahpF"
                     /locus_tag="PB780_002026"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDE0734071.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(4735..5298)
                     /gene="ahpC"
                     /locus_tag="PB780_002027"
     CDS             complement(4735..5298)
                     /gene="ahpC"
                     /locus_tag="PB780_002027"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDE0734072.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(5442..6104)
                     /locus_tag="PB780_002028"
     CDS             complement(5442..6104)
                     /locus_tag="PB780_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDE0734073.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            6288..7934
                     /locus_tag="PB780_002029"
     CDS             6288..7934
                     /locus_tag="PB780_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDE0734074.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            7925..9007
                     /gene="uxuA"
                     /locus_tag="PB780_002030"
     CDS             7925..9007
                     /gene="uxuA"
                     /locus_tag="PB780_002030"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDE0734075.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(9115..10668)
                     /locus_tag="PB780_002031"
     CDS             complement(9115..10668)
                     /locus_tag="PB780_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDE0734076.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            10816..>11626
                     /locus_tag="PB780_002032"
     CDS             10816..>11626
                     /locus_tag="PB780_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDE0734077.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEVAPEE"
BASE COUNT         3898 a         2173 c         2073 g         3482 t
ORIGIN      
        1 cagtagtgat gtatcacaag tagacttaaa aactgctttg atctattccg ataatatata
       61 tgcggcacaa gaaacgttga aaatgggtga gaaaaaattt cgtacaggct tagataaatt
      121 catttttggt gaagaccttg atttgccaat tagtatgaat ccagcacaaa tttctaatga
      181 agatagcttt aactcagata tcttgctagc tgatactgga tatggacagg gcgaacttct
      241 aattactcct atccagcaag cagcaatgta ttctgttttt accaacaatg gcacacttgt
      301 ctatcctaaa ttgattgcag ataaagagac aaaagataag aagaatgtca tcagcgaaac
      361 agcagtacaa acgatcgtgc cagatctgag agaagttgtg caagatgtaa atggtacagc
      421 acattctctt tctgctttag ggattccgtt ggcagcgaaa actggtacag cggaaatcaa
      481 agaaaaacag gatgtaaagg ggcaagagaa cagtttcttg tttgctttca atcttgataa
      541 ccaaggttat atgatggtca gcatgttgga aaataaagaa gatgatgatt cagcaaccaa
      601 acgagcatcg gaactattgc aatacctcaa ccaaaattat caataaaaaa ttgcttttta
      661 agtacagtaa aaggactgtg gcgttatcgt cacagtcctt ttttcaaata aacggttttc
      721 acgatgtctc tttcacaact tctttttgat ttactcacac ttcataaact ttttttgtaa
      781 aatatttgag aacaagtgga gaaataagtg tacttaataa aatcacgata actaaaggtg
      841 aatagtactg tggttcgatc aattgacttt gctgtccaat ttgtaaaatg atcaaagcca
      901 tttctccacg cgaaatcata ccagcaccga ccatcaatga actatgttga gaaaaaccgg
      961 caatctgagc accaaaataa cctccaagca atttggtcaa aatcgctaca agcgtcaata
     1021 tagaaataaa gagtagttgt tcactaaagt cagaaaaatc aacttctaat cctacactga
     1081 caaaaaatac tggtataaat actgcatatc ctaaagcctc tacattgtgg tatacttctt
     1141 ctttaacctt tgtctgactg acagctatcc cagcaaaaaa agcaccaatg acagagctaa
     1201 gcccaacaag atccgctacg taagacattc ctaaacaaat aactagcgat acgatgataa
     1261 tagaagaatt tgcgtacact ttttctgcaa atgaaagtag gaaaggcgca atccatttga
     1321 ctaataagaa aataaaaaca aaataaatta gctgttcaat caaaacaagc ggtaaagaag
     1381 cattgctaga agcttccccc gttagaaaag ataggctgaa acttagaact aaaacgacta
     1441 aaatgtcatc taccacagaa gcacccaaaa ttgttgatcc ttcttttgta ttcactacgt
     1501 ttagttcttt taatacttct actgaaatac tgacagaagt tgccgcaaga ataatcccaa
     1561 agaaaatcgc ttcatttccc gaaacttgga atgctttgcc tgtcaaccag ccaaaaacaa
     1621 ccgggaagat gatcccaagc aaagcaacga acataccagg tcggaaatat tttttcaaaa
     1681 gagataaatc actttccaga ccagctaaaa acattaataa gatcacacca atctccgaaa
     1741 agtcatggac tagaatatca ggtttgaccc aattcaatcc agcttggcct agcaaaacac
     1801 caacaagtaa ttgtccaatc actgctggga tacctaatct ccgcgagata tgtgagccaa
     1861 tcgttgttga aatcaatatc aggcataaaa taccaataaa ttccattctc atcattcctt
     1921 tgtataaaat atagcccgaa cttagcataa catacattat tgaaaaaata ggaaaatata
     1981 ctcacaaaaa aaccaacaat ccaaaagatt tttttggatt gttggttttt atacggtcta
     2041 ttttaataac taaaaatgat taacgtatag tcatttttct tattctgatg aaattgataa
     2101 tctcctggat gcccatgaaa atcaaaataa agccgaatat ttggaataga agcatcaaac
     2161 ttttgaatgg attgaacaat aagacgaatc cgccaatcaa caacagaata ctactataaa
     2221 cactccaact tttagctttt gtattcatgc tgaagaacaa ctggaacaag ctgttgatga
     2281 taacagctaa tccaagaaga atcggcagga tagaaacgat tgcaggtgca aaagcataga
     2341 cgatcagagc taagatcagg aaaaaaagac cactgaacag accgatactc caagaacctg
     2401 ttttcctttt aaaacggtaa gcctcaaaaa gattcaacaa cccaagcaaa acaagatagg
     2461 cggtgatcag ataaccgata aaatgaaaaa cagcagaagg gtttagcaca atcgcaatcc
     2521 cagaaataat ataaacagca gctcttaata aggcgtattt ttgaatattt tgtttaatcg
     2581 tctcaaacat ataagaaaac ctcctttgac ttattttaac gtgaactagt cattttacca
     2641 aattaaatgt tacaaaatga gcgaaggaga agaatatctc tggaaaaatc aacttctgag
     2701 gatcttttcc atttttttgc cttttgctag ctcatcgatc attttatcta aataacgaat
     2761 ttctttcatc aagttatcct cgatgtcttc tactcgtaca ccacaaacaa cacctgtaat
     2821 caaggaacgt gaaggattgg ggacaggggc ttccgcaaaa aattgttcga aagtcacttg
     2881 tttttgtagt tgatattcta gttctgtttg ggaataaccc gtcagccaac aaatgatttc
     2941 atctacttcc tcttttatac gatcttttct ctccgctttt gctacataca taggataaac
     3001 gcttgcaaaa cttgtcgtaa aaatacgatt ttgcgccata atcagtcacc tttctttttt
     3061 tcaaattatt tactacttat agtatataag aaaaagagaa gaaaaagcga ttattcaaaa
     3121 aaacaaaaag gattgtgaca aaattcttgt caccatcctt ttttccgctc tctgactttt
     3181 tacaatcttc atagctcaag tgcgcatcag ataatcgaat gctcctaaag ctgcagtagc
     3241 tcctgaaccc attgaaacaa tgatttgttt gtatggacta tctgtacaat caccagctgc
     3301 gaaaacgccg gggatattcg ttgcgccatt tttctcggtc actatttcat ttcttggatt
     3361 catttcgatc gttccatcta gccaatccgt attagggagt aagccgacta gaatgaatac
     3421 tcctgaaaca gctagtgtat gcatctccat cgtacggcga tcgatatagg agagagattc
     3481 tacctgattc gtaccgctga tttctttcgt cgctgcatgt ttgataacag tgacattcgc
     3541 aagagagact agtttatcct gtaagatttg atctgctttt aattctggta agaattctag
     3601 aacatataca tgttttgcta acccagccaa atcgattgct gcttcaacac cagaattacc
     3661 accaccgata acagcaatct cttttccttt aaataaaggg ccgtcacagt gtgggcaata
     3721 agcaattcct ttatttttaa actctttttc gcctggtaca ttgatagaac gccagtgcgc
     3781 acctgtcgcg atgatggcgg tttttgcttt aagtgatata ccgttagcta agccaatatt
     3841 aataaaatgt tgctctttat ttaaggaaac ggcctgttga ttcatcataa tgtcgatagg
     3901 gtaactacgc acatgacttt ctacttgctg catcaatttt ggaccttctg tgtaagaggt
     3961 accaatcata ttttcaatcc ctagtgtttc aacgacttgt ccgccgaaag aatcagcgac
     4021 aagtcctgta cggattcctt tacgtgctgc atagatagct gcactactag ctgctggtcc
     4081 accgccaatc actaatacat cataaggttc tttttcaaac aactcttctt caatcaaagg
     4141 gccagtgatt ttttcaacaa tttgttcgat cgtcatgcgt ccgctactaa attcttctcc
     4201 atttaaaaac acagttggca ctgccatgat ttttttatca tcgatttctt tttggaacat
     4261 cccaccttca atcatcgtat gagtaatatt tggatttaat acagctaata tattcaatgc
     4321 ctgtacaacg tcggggcaat tatgacaagt caagctaaca taactgtcaa aatgcaatgt
     4381 ttgtgttatt tttttgattt taagactcaa agattcactg attttcggtg cccgtccact
     4441 aatttgtaac atagccagta aaaaagagga aaattcatga ccgaggggga taccagcaaa
     4501 aacgattcca ctttctttgc caactggatt gatagaaaaa ctaggtgtgc gcatcgaatc
     4561 tcttttttcc caagagattt taggagacat ttctgctatt tccttcgtaa attcagctag
     4621 tttttgacta ttttcactat tatccaaact aacagaaaaa acaataggag attcaatcag
     4681 tgctaaatat tgttcgagat tttgtttagt ttctttgtct aacatatcca ccacctaaat
     4741 ctttccaaca agatcaaaac ttggtttcaa tgtttcgcca ctttctttcc attttgccgg
     4801 acaaacttca cctgggtggg tacgtacata ttgagcagcg cgaatcttat caatcagtgc
     4861 actagcgtca cgaccgattc catctgcatt gatttccata gcttgaacga ttccgtctgg
     4921 atcaataata aatgttcctc gctgagctaa tccttgctct tcatccaaaa catcaaaaat
     4981 acgagaaatc tgatgagacg gatcaccgat catcacgtat tcgattttac cgatagcatc
     5041 tgatgtatca tgccatgctt tgtgagtaaa gtgggtatcg gtagagcaag aaaagacttc
     5101 aacacctaaa gatttcaatg tactgtattg ttcttgtaag tcttctaatt cagtaggaca
     5161 aacaaaagta aaatcagctg gatagaagca aagaatactc cagttcccca taagatcttt
     5221 ttcactgact tctaaaaatt ctccttgatg ataagcatgt gcattaaatt cttcgatttt
     5281 tttcccaatc aatgacatag taaaacctcc aataataatt atttatttat aattattgta
     5341 atttagttgg agttgcaaag tcaatggata ttttgttatt taataaagat tactcaaaaa
     5401 cgaaggttaa ttataaaaat agaagaggtc gcaagaaaat gctactgctg gaaatactcg
     5461 ggataacgtt gagtaacagt ttcctgttct ttcagcatca tgtgtaagtg caatcccgtt
     5521 tgaaatcgag cttcttctgg ttttttatca actaaagcct tgagtaaatc ataatgttgt
     5581 tccatgatcg tttcccatgg aagcccctga atggtcactc gtaaccagcg gaaacgttct
     5641 aaatgggtat tatggtcatc taaccagttc caaacttctt ttcttcctgc aatttcgaaa
     5701 caagcttcat gaaataaatt atctgcacgg aaaaaagcgc gttcgtcatt tattttgact
     5761 gctttttctt gttcttctaa aatcgtttct agcactattc gtgtttgtgt gtccaatttg
     5821 gaacaacatt caatcatgat ttgctcttcc aactgttctc ggacaaagcg agcattttct
     5881 gcagaaacaa gattaatttt tgaaacatac gtaccacttt gaggtatcgt atacacgagt
     5941 tcttgctgac gtagttgaat caatgcttcg cgaatgggag tccggccgat agcaagatgt
     6001 tcctccagtg ccttttcaga tattttcctt ccaggttcta agtcagcata gataatttgt
     6061 ttacgaatcg cttgataagc ttgagattgc aagctgcttt tcatctttat tcctccaaaa
     6121 tcatttctat gaacgtagta taacagaaga ggtctgaaaa acaatctggt atgtcacatt
     6181 aactagtaaa tttgtataaa tagtaaaatt ttggttgaca tagcgatttt tattgcataa
     6241 aattcacttg aatacaaaaa ctaatatgtt agtttaggag gggaaaaatg attgatttaa
     6301 acaactggag gaactttatc cctgaattat ctcaaaaaga agtagaaata cctcattttg
     6361 accgtgcatc attaagagaa aatggacaaa aaaatcctgt ctggatccat ttcggtggcg
     6421 gaaatcttta tcgaggattt catgcagaaa tcgctcaacg tttagcggat ctaggcgaat
     6481 tgtccactgg tgtcattgtt tgtgaaacat tcgatgaaga aataattgaa agggcttacc
     6541 aagattttga tgatttgatt cttgaagtgg tcatgccaag cagtggagag ttcgtcaaac
     6601 gtatattagc tgcgacagct gacagtctgt attgtcatcc agattgttct gaacactttg
     6661 ataagatgat tcgacacttt gaaaatccta gtttacaatt agttactttt gctattactg
     6721 aaaaaggtta tcgattgaaa gatacaaata ataaatatat aggaccgatt gcagtcgata
     6781 taaaaaatgg tccaaagaag ccatcacata caatgagtat tcttgctgcg ctactttata
     6841 aacgtttttt atctggagct tggccgattg cacttgtctc tacagataat ttttcacaaa
     6901 acggtagtca attacgtact tctttgctaa cgatcgcaaa agaatggcaa aaggaaggtt
     6961 atgtacaaaa tacatttatc caatacttat cgaatgaaga aattgttgcg tttccatgga
     7021 cgatgattga tcgtatcacc ccgaatcctt ccttgtcagt tagcaaaatg ttggcaaaag
     7081 agggcttttc aaatatgtcg attttccaaa ccgcaaaagg aaataatatt gctccatttg
     7141 tcaatacaga ggacatccat tatttaatca tggaagatca ttttcccaat ggacgtccga
     7201 aattcgaaaa agctggagta ttcttgactg atcgtgcaac ggtagataaa gcagatacga
     7261 tgaaggtgac gacttgcctt aatcctttgc atacgtcttt agctattatg ggttgtttac
     7321 tggggtatag gagcatagct gaggagatga aagatccaga tttatcgctg ctagtttctg
     7381 aaatcgggta caatgaaggc ctcccagttg tggagtctcc aggtatcatt gatccaaaga
     7441 tctttctgga agaagtaatt actgtgcgct tttctaatcc aaatattcct gatatgcctc
     7501 agcgaatcgc cacggatacc tctcaaaagg tggcgattcg ttatggtgag acgattaaaa
     7561 aatatatgga aatagaagtt aaagacccgc agacacttgt attcatacca ttgactctcg
     7621 ctgcttggct tcgatacctt attggcattg atgatcaagg aaaaccattc atcccgagtc
     7681 cagacccact attgacagac tttcagcata agctttccga tattcaactg ggtacgcaag
     7741 atacatcaca tattcatgag aaaatggctc ctattttgtc aaataaagaa atttttggtg
     7801 tagatcttta ccaagcagag cttggcagaa aaatagaaga aatgttcatc aagatggtag
     7861 caggaccgaa tgccgtacga aaaacattac aggattactt acaagaacat ggaggaaatt
     7921 tatcatggaa atgactttta gatggtacgg atcacaggaa gaaaagatta ccttagatga
     7981 aatcagacag attcctgcgg tcaaagggat tgtaggcaca ttgatggata taccagtagg
     8041 agaggtatgg cctagggaga ggatcaaaga actaaaagaa gaaattgagt ctcgtgggtt
     8101 aacactaaaa gtaattgaaa gcgtcaatgt gcatgatgcc attaaaattg gattaccttc
     8161 ccgtgagcag tatatcgaaa actataagca gacaatccga aacttggcag aatatggaat
     8221 agaagtcatc tgctataatt ttatgcctgt atttgactgg gtgaaatctg accttgacta
     8281 ccgcttagaa gacggctcat ccacgttagc ttttatatct gatgatattc cagatgatcc
     8341 aagagaaatc gtcgcgcgca tcgaacattc cagcaatgaa tttgaattgc cgggatggga
     8401 acctgagcgc ttagctcata tcaaatcctt gcttgaagcc tatgcatcag tagacgaaga
     8461 aaaacttcga gaaaattttg cctattttct ccaatcaatc atcccaacat gtgaagaggt
     8521 aggggtcaaa atggctgtac atccagatga tccgccatac tcgatttttg gattacctcg
     8581 aatcgtaaag aatcgagaag atttagattg gttgtgccgc atagtagaca gcccttataa
     8641 cggaatcact ctttgtacag ggagtattgc agaagatcca gaaaatgatg tatatgggat
     8701 tttagcagaa ttttgtcaaa gagatcgaat cccatttgca catgttcgaa atattaaatt
     8761 tttagctaat cgagatttct atgaggcacc gcataaatcc gaatacggtt cattagacat
     8821 gtaccgcata ctaaaaacaa tgtatgacaa cggatttaac ggatacatcc gtcctgatca
     8881 tggaagaatg atctggaatg agacaggaag accaggatac ggcttatatg atcgagcatt
     8941 aggtgctgtt tatctaaatg gattatggga agcaatcgaa aaaggaaaca aaagtaaaga
     9001 taattagtga aaaaaactgt aattttatta tgtaaataag aaggatgttc aaattcttga
     9061 tacataaatc cggaggttgt taataagcat tcggatttat gtatcttatt tggtctagga
     9121 agtaacttga ttttcttttt tcgattgatc catgagtgct tcttctttta attcaaagat
     9181 atattctgag atcctcataa tcactttttt ccagtcttct ggtgtgatga ttccttttaa
     9241 aatatagatt tttttgatat gagaaggcaa ttgtttcaaa gggcagtcgc tgataacgat
     9301 gttgcagttc tccataaaat cattttgtat tttgatcgat tcatttgaaa aaatttgttc
     9361 tatcttaacg atcagatctt tactgatata aaaatcacgc gtatagttca caccaatcac
     9421 aattcgcggc tgttttttgt agcgatcata caaatgtact aggtcttcaa ccatccattg
     9481 cacattttcc ttttctaaaa taactggtaa caagtgttga ttttgttctc tgaaatccat
     9541 ataaaattgt tcaatggcgg tttggacttc tttcaacacg ctatgttctt gataaagcat
     9601 gaaagtagga aggggcgtat ttggttgatt gaagttaaga aatccattat aaagtttatt
     9661 tcgtgcgaat gccaatgagt aaagaatctt ttctttttca ggtataaaca aatcaaatac
     9721 agtacaaaag gactccacca tttctcttga ataaagaaca tgaggaacgt ttgctttttt
     9781 taaaagtttg tattgctcca agcttgtttc ttccgattcg attttcgtga tagaaagtcg
     9841 agctaaaata ttcactaaga tgcgttgttc gcgacaaatt ggcagattag gtcgcaaaat
     9901 atctacagag ggatcaataa aaaagtcaat atattcatac tctgtgaatt ctttttgtac
     9961 gtctttcaca gaagttgcag gaaaattctc tttacatatt tttaatagaa aatataattt
    10021 atctatcata ccgctattta gtcggtcaag tacttctttt ttaaaaaaag tattcatcat
    10081 aaaagccact acttgcggat cttcatcata aaattctgta gcagtacttg aaaagatgtt
    10141 ccaataaata tctaatgctg catattgaat ttgtacctct tttccagaaa aagactttct
    10201 gccattatta gaaaaggaga tagacaaacc gtacaatgct aaaaatgcat taatcttttt
    10261 cattttcgta tacaaataag aggcactata gtttgtttgc tgacttaaac tgataatcga
    10321 atttgtctga cagctgaaca aagaagtcaa gatctgataa ctgactgatt cgttgcagta
    10381 tcttccaaat agacgataat agcagtaagc cggatcaatt ccttcaaaat tcaactttaa
    10441 aaaaggtttt tgcttatcaa tatgcagttc tgttagattg agacgctcaa agtcttgttc
    10501 taattctttg atgtatagta aaaaatttga ttcagacata tggaaatgat ctgctaaata
    10561 ttgtgtagta ataaagaaac gatgttcaga taggaaaaga aaaatatcta attttgtatt
    10621 gctttttttc ttcaagagaa acgttttaat cgcttcttga gtttgcatga aagaggctcc
    10681 tttttactta aattgttaaa acttgaaaat attactgttt ttttcataaa tttagacaaa
    10741 aaaacagtaa acatataggt agaatctcta ctatcttatt tcttgcatac aaaaaaatca
    10801 aggagtgaga gagttatgag atattcaacc atcttaatat caacatttgc agccttaagc
    10861 ttatcgctta ctataaatca gacagtccac acagaggaga tagaaacagc caaatggaca
    10921 gaaaccaagt gggccaacgt caatcgaatt acctttcagg atgaattttc atccttcaat
    10981 tcaggagtcg caaaattaag aaaaggacaa acagcgacca tcaaagcaaa aattgactat
    11041 tcaggagact tagcacctat actacaatca gctgttacat ttgagaatgt tgatcccgct
    11101 ttgtcgattg gctatgacga gaacactttg actatccaac acaagcaagc gatatttacc
    11161 ttcaccgtta cattaaatca ggacttgtta aaaccagcgc tttttactgt caaggtatca
    11221 gatggactac cgaacgatca tcatcattta acgccgtata gtcagcggca gacaattgag
    11281 caagattcag ctatcgactc aggaggagat cttgttgaag aaccaacaga taaaccagaa
    11341 aatgaaaata agccagaagt accgcctaca gaaaatccag acggagaaca gaaaccggaa
    11401 atagaacctg gagaagaacc cgatacagaa actcaaccag agccagacaa tgaatcaaaa
    11461 cctgaaataa cccctggaga aaaaccagat gtagacccag aagaaaaacc ggatgtgact
    11521 ccagaaccag atacggattc tggaaaccaa acagtaccgg aaacaaatcc agacacagat
    11581 aatgaaactg aaaatccaga aaaaccagag gtagccccag aagaaa
//
LOCUS       DAMEET010000078        11514 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.079, whole genome shotgun sequence.
ACCESSION   DAMEET010000078 DAMEET010000000
VERSION     DAMEET010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.079"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(79..2154)
                     /locus_tag="PB780_002033"
     CDS             complement(79..2154)
                     /locus_tag="PB780_002033"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDE0734078.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDIKTDRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKDHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEV
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2156..3073)
                     /gene="glyQ"
                     /locus_tag="PB780_002034"
     CDS             complement(2156..3073)
                     /gene="glyQ"
                     /locus_tag="PB780_002034"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDE0734079.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRF
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARSVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3457..4269)
                     /gene="recO"
                     /locus_tag="PB780_002035"
     CDS             complement(3457..4269)
                     /gene="recO"
                     /locus_tag="PB780_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDE0734080.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFLSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHAYPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKMPEKNMPEKEIPEKEE"
     gene            complement(4412..5311)
                     /gene="era"
                     /locus_tag="PB780_002036"
     CDS             complement(4412..5311)
                     /gene="era"
                     /locus_tag="PB780_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDE0734081.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYTALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5326..5721)
                     /locus_tag="PB780_002037"
     CDS             complement(5326..5721)
                     /locus_tag="PB780_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HDE0734082.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQIDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5705..6181)
                     /gene="ybeY"
                     /locus_tag="PB780_002038"
     CDS             complement(5705..6181)
                     /gene="ybeY"
                     /locus_tag="PB780_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDE0734083.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6198..8390)
                     /locus_tag="PB780_002039"
     CDS             complement(6198..8390)
                     /locus_tag="PB780_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HDE0734084.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNTGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8414..9385)
                     /locus_tag="PB780_002040"
     CDS             complement(8414..9385)
                     /locus_tag="PB780_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDE0734085.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10090..11469
                     /gene="brnQ"
                     /locus_tag="PB780_002041"
     CDS             10090..11469
                     /gene="brnQ"
                     /locus_tag="PB780_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDE0734086.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
BASE COUNT         3111 a         2419 c         2104 g         3880 t
ORIGIN      
        1 ttatttatat ttataacaaa taaaccagag aaatagccta ttttgtgcct gttctctggt
       61 ttatttacat attagaaatt attttgtgat gatttgatct aaacttgcta gtgacaaagc
      121 catggcagca atttgactta attgcgttaa tcggttctta cgtactgctg gttcttctgc
      181 catgaccatc gtctgatcaa aataattttc gatcagtggg cgaaggctta caagctgatc
      241 ataattttcg ttgatcgttt ggtcagcaaa gcttgttttc attacctcta ctgctttgtg
      301 cagttctttc tccgcttcgt tttcgaacag ttcagggtct acttctgatt gtttttcaag
      361 ctccgctttt ttcgctaagt tcacgacacg tgtcaaagct tccatcgatg gtttgaagtc
      421 ttcatctgca agatgctgct tgaacaattg tgctgctgca aatacttttg tcagatcttt
      481 ttgttctgcg ttcaaaacag cttcgatgac atcatgacgg atattcttcg ttgtcaataa
      541 ttggcgcaga cggcctttga taaagtcgat cacctgttgt tgtcccgctg acaagccaat
      601 gccaaaacga tccgtatctt tactgataac ttcactaatc gtttcttgca gaacagacaa
      661 aggaaaggcc cattttttcg attcgatgat tcgaacgaca ccatatgctt gacggcgcaa
      721 agcatatgga tcgtttgagc cacttgggat catcccaact gcaaagaaag aaaagatact
      781 atctaattta tcagcaatcg ctaaaacaga acctactgta gtctcaggta aatctccttc
      841 actggatata ggcatgtaat gttcacggat cgctgttgct acggctggtt tctcgccttt
      901 aagcagagca tatttttctc ccatgatccc ttgtagttca gggaattctc ctaccatgtt
      961 ggttactaga tcaaatttat agatttcagc agcgcgttgc agatccatca attcttcctc
     1021 agacagtcct acttgttcgc ctatgatgcg agcgatgact tgtacccgtt gcattttctc
     1081 atagatgcta ccgatttttt catggaaagt aacagttttt aatttttcaa cgtattggtc
     1141 gattgaagct ttcttgtctt cattataaaa gaattccgca tcttctaaac gagcagtcaa
     1201 tactttttga ttgcctcgta ccacattttc caattttacc cgatcgccat tacgagcaga
     1261 aataaaatga ggaagcaaca taccttgatc gttccgtact tcgaaataac gttgatgttc
     1321 tttcatagaa gtgactagta cttcttcagg aacagataga tatttttctt cgaagtcgcc
     1381 agaaaagaca gttggatatt cgactagatt gttgacttct tctagtaaat cttcatctag
     1441 atcaatgatc cagtgatctt tttctgctaa ttcgtttgct tgtgtgatga tcatttcttt
     1501 tcttttttgc ggtgaagcaa taacaaattg ttcatttaat ttttcttcgt attcttttgc
     1561 atgcgcaaat gtcacatctt cgcctaagaa acgatgacct cttgaagctc tgtctgtttt
     1621 gatatccaag atctcaaacg ggattacttc gtcatctaac aatgccacga tccagtgaat
     1681 aggacggata tattcaaaat cgtattctgc ccaatgcatc gttactggga aagtgaggct
     1741 tttaatgaca tctgctaaac ctgtcaatac ttcttttgct tctttgccgt gagtgtattt
     1801 cgttacatag acatattcca cgccttttaa ttctctaaaa gtgatagcat ctgtcgtcag
     1861 accttgtccg cggacaaatc cttccgctgc tttcgtccag tttccatctg cgtccaaagc
     1921 aatttttttg gctggtcctt tgacttcttc ttcactgtct gcttgacgtt ctggaatgtt
     1981 tgttactttg attgctaaac ggcgcggtgt agaaaatgtt tcaatcgtat cataagtcaa
     2041 ttggttttca tctaaaaatt ttgctgtttt ttcttctaat tgtttcatgc ttggtgtgac
     2101 aacatgagca ggcatttctt ccagcccaat ttcaagtaat aagtcttttg ccatcttatt
     2161 cagcctcctt cacgttttct ggatttgctg tgtttttatt caataaaggg aatcctaatt
     2221 tttcgcgttc tgccacaaag attttggcta cagaacgagc catgttgcgg atacgggaaa
     2281 gataacctgc acgctccgtt acggatactg caccgcgggc atctaataaa ttgaatgtat
     2341 ggctgcattt caaaatataa tcatacgctg gatgaacaag attttcatcg atacagcgtt
     2401 tagcttcttt ttcaaaacga tcaaagttgc ttagtagcaa gtcttggtcg cttacttcaa
     2461 aagaatattt ggaatgttca tattctggtt gtttgaagat ctcgccatat ttcacgccat
     2521 cagcccattc taagtcatag acactttcaa cttcttgaat atatgaagca agacgttcta
     2581 gtccataagt aatctcagaa gtaactggtt tacaaggtag tcctccgact tgctggaagt
     2641 acgtgaattg tgtaatctcc atcccatcaa gccatacttc ccaacccaca ccggcacaac
     2701 ccatcgatgg gttctcccag ttgtcttcaa caaaacgaat atcgtgttct aatggatcaa
     2761 ttcctaaaaa acgcaagctt tctaaataaa gctcttggat attttcaggt gaaggtttca
     2821 ttacgacttg aaattgatga tgctgataca aacggtttgg gttttctcca taacgcccgt
     2881 cagctggtcg gcgggatggc tctacataag cagcattcca tggttccggt ccgattgccc
     2941 gtaagaatgt atatgggctc atcgtacctg ctcctttttc tgtatcataa gcttgcatca
     3001 gcatgcagcc gtttgatgac caaaattttt gtaatgttaa aatcatatct tggacggtca
     3061 attttttact catctgtttt gcccctttca atttttaagc gttgctgtca ataagaatac
     3121 aggaaagaaa acgacaaaaa gtccctatgt ctgattacag acatagggac gaataatttc
     3181 gcggttccac cctacttccg aataaagatt cggcagcttc atttatcgta ctcgcaagtg
     3241 cctcttcaaa aatcgtttgg gtccggcttc cactctcccg aactcgctaa accacgtgtt
     3301 ttttactgat cttggtcaat gtacgtattc attttgcagt tttcttgttt atataataga
     3361 tcctctcagt gaacgatcta tttaatctac ggttaataat agacggaagt ccctgtattt
     3421 gtcaaatagt tttgattttt ttgttgtatc tttcatttat tcttcttttt caggtatctc
     3481 tttttcaggc atatttttct ctggcatctt taatacgtgt gcccaagact tcatttgatc
     3541 aatgaacttt ttgcttttta gatgaatacc gacatattca tcatacaact ggtcaatgag
     3601 gtcacggatt gcctgcttcg tttcttcttt caagtggatt ccactgatct tatcataaga
     3661 aatagccgaa aacattcgaa cgaaataaac agcgcgagga tatgcatgat aacggtgttc
     3721 gtccattggc cagtgtttct cgcatatcac gccgccatat ttggaagaat agtcaaattt
     3781 tccttttgtt tccccgcata cgacacagtg cgtccaaatt ggcgcgattc cgaagcgttg
     3841 cagtatttga acttcaaaga tattcgtgat gatttctgca tcatctcctt ggtccatccg
     3901 ctgcagagct tgttgcgtaa attggaataa gtgcgggtca tattgatgat cttcgattgc
     3961 tgcatctact agatttagca aatacgtacc ataaccgctc aaaaagatat cttcttggat
     4021 gttccgaaac gcatggacgt ctttgacact gcttaaaaac gacaaacctt cacttagaaa
     4081 attaccgata tagactgctt cggtaaaggg gagcaaagcc ggagctaatg gctgattttg
     4141 ccgatggatt cctttgatat aaaacatcat tttgccagaa gattccgtaa agattttgac
     4201 taatttatct ttttctttgt aattacggct agataaaatc aagcctttcg attctgcaag
     4261 ctggctcata ttctccctcc taaaaaataa atctggaaaa gcagattctg ccgatccaga
     4321 ttatttatgt tacgatcttt cgctgttttt ctcaacgtac ggatttttat ccagtttatt
     4381 tctataccta acctataaga agtccagaat atcagtactc gtcttttcgg tagccgaagt
     4441 cttgcagata gacttttttg tctcgccagt ctttttggac tttgacccaa agttctagaa
     4501 atactttgtc tcctaaaaga ttttcgatat cctttcttgc tttcgttcct acttgcttca
     4561 gcatttttcc acctttgccg ataatgattc ctttttggct atctctttca acgatgatcg
     4621 tggcctggat atggatttta tcattctcgt ttcgcttcat cgaatcaaca acgactgcga
     4681 cagaatgagg cacttcgtct cgtgttaaaa acaagacttt ctcacggatc aattcagaaa
     4741 cgatgaagta ttcaggatga tcagtcactt gatcatctgg aaaatattgt gggccctctg
     4801 gcatttcact gactaataca tccattaagc gttcgacatt gtttccttct gtagcagaga
     4861 tcggaatcac ttgtgcaaaa ggcatctgcg tcgaataatc ttcgatgatt cccagcaact
     4921 cgtctggatg gatcttatcg attttattga taatcagata aactggagaa tgcacgtttt
     4981 ttagacgttc aataataaaa tcgtctcccc ggccgcgttt ttgatcagca ctgatcataa
     5041 ataagactgc atccacttct ttcaacgcag tgtaagctgc ttctaccata aaatctccta
     5101 gacgatgctt tggtttatgg attcctggtg tatcgataaa aatcagctga gcttctggtg
     5161 tagtatatac tccttggatt ttatttctag ttgtctgtgc cttgtcactc ataatggcaa
     5221 ttttttgacc cacgattcgg ttcaataatg tggattttcc tacattcggg cggccgacga
     5281 ttgcgacaaa ccctgattta tgttctggca tatacgttct cctttttaat taaacaacaa
     5341 atgatatatt ttagggacaa ataaaatcaa cccaatgatg acagcaaaac aagcggtcac
     5401 taaaactgct ccagctgcca tatctttgat ttttttccca atcggatgaa aatgaaaatc
     5461 cgtaaacatg tccactacat tttcaaaaac agtattgatg atctctacta tccaaacgag
     5521 aaaaacagct aaaagcagcc ataaccactc tatccgatca atctggaaaa tcacgcccat
     5581 aaataaagca aggataccga acgcaacatg tttcctcata ttgcgttctt cctcaaaaac
     5641 ggttttgact ccttggacag caaattccac ggacatcagg aaatgtttgt ttttctctac
     5701 gtttttatct tttgagtcca taggcatcca agatctcttt ctgtaaacca aacatttctt
     5761 tctcatcttc tggtgtcata tggtcatacc cgttgatatg caagaatcca tgtaccgcta
     5821 aaaagccaag ttcccgatca tatgaatggc cgtaagcttc tgcttgttca gcagcacgtt
     5881 caatagagat catgatgtct cctagtacac gaggcaatgg attctctcca tcttcaaaaa
     5941 tcactgggat ttcgtcttcg ccttcttctt ccagagcaaa actgatgacg tctgtcggtg
     6001 catcttttcc acggtaatca cgattgatta cttggattgc cgcattgtcc atgaaagtaa
     6061 cagacatttc tgtttcctct ggcagcttta aataagatgc tgcaaattgc agcaagtcat
     6121 cgatttcttt cattttctct tctgagactt ggtttgtttc atccataaag gtaatatcca
     6181 ttatacatcg cttctttcta ttcttcttgt tcttccttta tcttctcaac ttctgttttc
     6241 attttaggat atttgatccg agaatggaag gtgctactta aaccgctgat caatgatttt
     6301 tccacttttc ggatttcttt caaggtcaat ccactatcgt ctaattgacc atcggaaata
     6361 cgctcttcga taagattatg gacaaaactt tcgatttttt cgctcgttgg atgatccatt
     6421 gcccgtactg cagcctcaca actgtcagca atattgacga tccctgcttc acgactttgc
     6481 ggttttggtc caggataacg gaattgctct tctgttactt ccggattgcg ttctttcgct
     6541 ttgatgtaga aataacgcat tagcgtggtt ccatgatgct gtttgcaaat atcgatcacc
     6601 atttgcggca tattataatc ttccaatatt ttggctccat ctgtcacatg tccaaagata
     6661 atatgtttac tgtcttctgg caataagaaa ttgtgcgggt tttccgctcc tgaaggcaag
     6721 ttctcaacga aaaagctagc atgtttgatc ttgccgatat catgataata gcaagctact
     6781 cgagttagta aagaacgtcc gccgatttct gctacagcgt tagcacttag attagctacc
     6841 atcatactat gatgatacgt acctggcgct tcttccagca actgtttcaa taaaggatga
     6901 tttggattgc tgagttcatt taatacaatt acactgtcat cattgaccat caattcaata
     6961 tatgggtgta gaccgatcgt cagcaagaat gagaagagtg caccagtgaa cccgcagaca
     7021 agcattaacc aagtcgttgc atcaccaaag ctcattcctt gatagacgat caaaatgaca
     7081 tccatcatca caggaaaaat aatgacccac atggcagcag ataatccttg ttctgagatc
     7141 cgtttacgtc tgacaatcgt tgccatgagc ccagagaaaa cataagacat caagataatc
     7201 gttagaaagt tcgtgccaat cgaatcataa aaaatgaaaa gtgcggatac tgcttggaaa
     7261 ataccagcca tgatccctgc tcttcgattc aagaaaaagc ttaaaacaag tggtgcaaaa
     7321 gcagcgggat aaaacagcgg gatataagta gcttgttccg tttggaataa ttggaaaaat
     7381 ttcatcagca ctatgctgac tgacatagct gtcacataga aaagaacgta ttttgttctt
     7441 tctcctgtat tttggaattg aagtgaatta taaatcagta cgccgatttg aagcaaaaca
     7501 gctaaaacca tggcaattag cggaaaaatc gaagttgttt gattagtcag tcctagtaat
     7561 tccaattttt tcatagctga agcatcaatt tgattacctt cacgaacaat gatttctcct
     7621 tggaagatca tcactggttg tacagcttct cgtgcggatt gtttcaattc ttctgtcttt
     7681 ttctcattca atacgtcatt cactacgata ccttgatcca ctaagtagcg aatcatttgt
     7741 tgttgtgctg gtgtgacatt caaatattgg atttgctctt ctgcttcttg tttaaaagct
     7801 tccaattcac tttcacgaac ttgtttgctc atctgttcgt caatgagctt cagactctca
     7861 tccctcacag tatgaatctc agtttcagtc atttcaaata tcgtcgaata aaaattgttc
     7921 ggtagttttt gataaaacac aacattttct gcattcacat tttcaaaatt tttcttcagc
     7981 gcggcgatcc gctcttctgt agtaggcttt gggatatttt catcttcctt tgccttatca
     8041 atcttttcgt cataagattt gttgatggca tcattcgtct tatcgatcaa ttcaaatagt
     8101 tgcttgatcc gattgttctg gtcatctgca agatcttttt ggtaagtgta ttctggagtg
     8161 actgcttctg ctgccagttt tcttttttgt tcggtttctt ccgtgttctc gatcgttttg
     8221 ttcgcacgga tactttcttc tgctacttgg ccttctctga aatcattggc cttttgtttg
     8281 acactactaa atgtaacgcc gcagataatg ataaaaaaca agaacagcag gaaaggcaat
     8341 gctgcctttc caagtttttc ttgtagtttc tgcagtgttt tattcaacat gtgcttatcc
     8401 tcctaagcaa agcttattct tgatggagat ctgccttttc ataagctttg atgatttcgg
     8461 caactacagg gtgccgaaca acgtcacttg cttcaaaatt aacaaaatcg attttcttga
     8521 tttgctttaa tgttcgttct gcatgaacaa gaccacttgt tgtccctcga ggcagatcaa
     8581 tctgactcgt atctccattg acaatcattt ttgattgata accaagacgt gtcaggaaca
     8641 ttttcatctg agcaactgtc gtgttctgtg cttcatccaa aataacgaat gcatcatcca
     8701 atgtccgccc ccgcatgtaa gctaaaggag caatttcgat cacgcctcgt tccatcaatc
     8761 ggttcgtatg atctaatcca aaaatttggt ataaagcatc ataaacagga cgcagataag
     8821 gatctacttt ttcttttaga tccccgggaa gaaagcctag attttcccct gcttccacag
     8881 caggccgcgt caaaatgatt ttttgcactt cacctttttt caaggcagcg attgctaata
     8941 ctactgcaag aaaagttttt cctgtcccgg ctggtcctac gccaaatacg atatcatgtt
     9001 tggctaccga ttcgacatat ttcttttggc cgctgttttt tacccgtata ggttttccat
     9061 tacggtcttt gacgatctct tcctcataca tctcaacaaa ataatctaat gtgccttttt
     9121 gccccatttt cagtgccgtc acgacatctg gtgtacttac atggattcct cgtttgatca
     9181 attcctgcaa tgtccgaatg actgaggcag ccaaggaaat ctgggattgt tcgccaatga
     9241 tctgaatgat ttctccacgt gtatggatgg ttgtttctgt tgtttcttca atcagtttta
     9301 tgtgcttgtc gtgtgcacct aaaagcatgc tgacatcatc agcagccgtc aatctaatct
     9361 ctaaagaact agactcttcc gtcaaaaata gacatcccct ttttttcgat tcattctttc
     9421 ctattttacc atactcagaa gaaactatat agcaaataag aaaaggttct aaaagagttg
     9481 cttaaccata agtaccaaga aacgttaaaa aatagctgct tccattctct aaaggtctcg
     9541 tttgaataaa aaaagctgat catgtaagct atgcataaga tagaggacaa gtattccgtc
     9601 taacttagca agtattctct gggtattttt cttgtttcaa aaaaagaaaa caaaatattt
     9661 gaaatattct tgcatttcaa aaaaaacagg tgtattataa cgtcaacaaa taaatataca
     9721 gcaaagcgtc gaggaaaaca gtaggctatt cagtgattta ttcccagtga gtcatgtaag
     9781 tgagaaatga caataaatcg aatagacgaa catcatttcc gagtcaatgt cgaaaacgta
     9841 gatcattgtg gctgcctcca ttatcaggcc agcggataac tttatcgaaa agcgtccgtg
     9901 aatgaagaag cttttttaag cgaaaaaagg tggtaccgtt ctagaatcta gagccctttt
     9961 cccaagtgga aaagggcttt ttttatattt taaggtggtg gttttcatgg aatctgatat
    10021 tagtggtatt tctagtttct gcaacgttta ttttgttact caaaatgata attattggag
    10081 gaaaaattta tggaagaaac aaaaacttat tcatggaagc aattattgat ggtcagttca
    10141 cttatcttcg ggatgttctt cgggtcaggt aatctgatct ttcctgtcca cttaggtcaa
    10201 atggctggat caaactggat cagcgctggt gtaggatttg ccatctcagg cgctcttttc
    10261 ccattattag ctatcttagc tgtagtagtg acaaaaagtg atggtctgta tgacttagct
    10321 aaacctgtcg gaaaatggta tgctgccttg ttcttgatcc ttgttcattt aacgatcgga
    10381 ccattctttg gtacaccgcg gacagcagca actgcttttg aaatggctgc taaacccttt
    10441 ttgcctgaaa aatatgattc attaggtatg tttttattct ctgctctttt ctttttactt
    10501 gcttatttcc ttactgtaca tccaacacgt ttggtcaaat atgtcggaaa gtatttaaac
    10561 tccttgtttc ttgcattatt agctatcatt tttgtcgttt ctttattcca acccatgggg
    10621 aatctaaacc aatctgctgc agattcttat cagacgaacg ctttgacaaa tggcgtttta
    10681 gaaggataca acacggttga tgcagtagct ttacttgctt taagtgtcac ttttgtccat
    10741 gcagttcgtg atcttggttt taaagataaa aaagtcacag aattaacagc aaaagctggt
    10801 agcctgagcg ttgcgttaga agtcatgatt tattttggtc tggtccttct cggtgcgatg
    10861 agtttgaata agatgtcttt atcagaaaat ggcggaactg cactttctca gatcactaat
    10921 ttttatttag gtaacttcgg tgctagcttc ttaggggtaa tggtcacatt aggtgtcttc
    10981 actaccgcta tgggactcgt tgtttctttt gcacaagatt tccataaact gttccctaaa
    11041 gtcagttata tgacatggct gcggttaacg acttttgtct cttttgtcgt tgcgaatgcc
    11101 ggattggata atatcatcca atggtcttta ccagtattga tgcttttgta tccattgtca
    11161 cttgcgttaa tcttgctttc cttaacagca aaattcttcc aaaagagtcc atttgtctat
    11221 caagtaacga tgttcttttc tgccgttcca gccgtattgg atatgttggc aaattcgcct
    11281 gcattagtga gtcagcaaac tgtcgtagca agcttactag gattttatca tcattatgtt
    11341 ccttttgctg cattaggact tggctgggtc gtcccaacgt tgcttggtta cgctggcagc
    11401 ttgctctttt attatgtata tcgcgtatca ggatataaac aagaagcaag tgagttacca
    11461 gaagaataac caaatcaaac acaatttaac ataagagata ttaaacagga aaaa
//
LOCUS       DAMEET010000079        11315 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.080, whole genome shotgun sequence.
ACCESSION   DAMEET010000079 DAMEET010000000
VERSION     DAMEET010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.080"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            141..746
                     /locus_tag="PB780_002042"
     CDS             141..746
                     /locus_tag="PB780_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HDE0734087.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            762..1334
                     /locus_tag="PB780_002043"
     CDS             762..1334
                     /locus_tag="PB780_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDE0734088.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1586..1843)
                     /locus_tag="PB780_002044"
     CDS             complement(1586..1843)
                     /locus_tag="PB780_002044"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734089.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(1969..2784)
                     /locus_tag="PB780_002045"
     CDS             complement(1969..2784)
                     /locus_tag="PB780_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734090.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            3301..4626
                     /locus_tag="PB780_002046"
     CDS             3301..4626
                     /locus_tag="PB780_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDE0734091.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            4619..4969
                     /locus_tag="PB780_002047"
     CDS             4619..4969
                     /locus_tag="PB780_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734092.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            4951..5136
                     /locus_tag="PB780_002048"
     CDS             4951..5136
                     /locus_tag="PB780_002048"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734093.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
     gene            complement(6040..6315)
                     /locus_tag="PB780_002049"
     CDS             complement(6040..6315)
                     /locus_tag="PB780_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HDE0734094.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(6287..7240)
                     /locus_tag="PB780_002050"
     CDS             complement(6287..7240)
                     /locus_tag="PB780_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDE0734095.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            7852..9345
                     /locus_tag="PB780_002051"
     CDS             7852..9345
                     /locus_tag="PB780_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HDE0734096.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            9480..9776
                     /locus_tag="PB780_002052"
     CDS             9480..9776
                     /locus_tag="PB780_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HDE0734097.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            9877..10728
                     /locus_tag="PB780_002053"
     CDS             9877..10728
                     /locus_tag="PB780_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734098.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            10749..11032
                     /locus_tag="PB780_002054"
                     /pseudo
     CDS             10749..11032
                     /locus_tag="PB780_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3904 a         1755 c         2091 g         3565 t
ORIGIN      
        1 tagactgtgc aaaatagaga cacttgaaaa atctatcggg ggtcagaaac ttaatagttt
       61 ttgacacata aacaaaatcg cgatataatg gggttgagag tgtttcataa acttatgtca
      121 taaagttaag gagatttcgc atgattttag agaataaatt gggattaacc aatcaagttg
      181 agttggctaa ggttgaagaa aaactcagta aacaaaaagc gaaagaattg tacgattgtg
      241 gaaaaataaa tgaaatcgaa gttggtacat ttaaaggttt atctgaaatt catgaatttc
      301 tattttcgga tatttacgat tttgccggaa aaatccgatc ggtcaatatt gcaaaaggaa
      361 attttcgatt tgcaccagtt atgtatttag aacattcgtt acagcatatt gatcagatgc
      421 cacaaacttc ttttgaagag attatcaaga aatatgtgga gatgaatatc gctcacccat
      481 ttcgagaagg aaatggccga agtactagaa tttggctgga tctcatttta aaggaagaat
      541 tgcaaaaagt tgtagattgg aacttaatcg ataaagcaga ctatctttct gcaatggagc
      601 gaagtcccat aaacgatttg gaaatacgct atctaatttc caatgctctg actgataaaa
      661 ttgacgatcg cgagttatat atgaaaggaa tcgatgtcag ctatttctat gaagggtatt
      721 ctgagtatac gatagatgat ttgtaaaagt aggtggacca aatgacgaaa tatggatatg
      781 cacgtgtctc aaccgcttct caaagtacaa aagaacaaat tcaacagttg cttgagaacg
      841 gcgtagagaa gaagaatatt ttttcagaaa aatttacggg tacaaaacgg gatcggccaa
      901 aattcaatca gctgttggct ggtctgaagc ctggagatga aatagttgtt acgaagcttg
      961 atcgatttgc tcgtaacacg agagaagcat tagagatcat tgaaccttta ctcgatgata
     1021 acgtaacgat caaggttttg aatctaggaa cgattgaaaa tacgtccatg gggcgaatgg
     1081 tgacacggac gttgttgagt gtagcagaaa tggaacgtga tatgattgtg gaacgtaccc
     1141 aagaaggaaa aatgtttgca agaaagaata atcctaattt cagagaagga cgtccgaaag
     1201 caacaatcac accgaagaag aggcacgcat atgaactgct tacttcaggt aaatcgtata
     1261 aggaagttga agctataacc ggtttcagtc gcagtacgct ttttagaata aagaagaaaa
     1321 ttgaagaaag ttaaaaaaga agtctcagcg aaccagacag gtaaatactt aatgcgttct
     1381 agatatgagt ctattagcat gttgtgaaat ataatatttt tttatttact tgatttctgc
     1441 tcaaaaaatg tgtatggtaa ctgtaaaagg taaagtaatt aaaggtatgc tagccacgct
     1501 tactatatta ggataccatc atatatttat aatgaacgat ttgttcgtct accaaacaac
     1561 acatatcgtt cagcgtaagt cacgtttagg aacgtggctt ttttagtgtt attatcgata
     1621 aataatctat tgactctatt gattttatct ccatatccct acggtgttga ttgtctttag
     1681 atctttcatt taagaaccaa ataactatca taaaaatcag cactgctgct aatataataa
     1741 acagcattcc tattgtgata ttacctattg attttaccat aactacgatt aaagtaataa
     1801 tggcattcaa gtctcctttg ttctctatat ataaattagc cataaatcat cttcctttca
     1861 aattaagata cttgtttgat accaataaat agtaagcgtg gctagcatac ctttttctgt
     1921 aatagcaagt gttctaatag tccaaactta atgatggacc gatgctagtt atagtacagt
     1981 aatgttttct ttcttatcta gatttttagt aagctctatc agcaaatcta acaaatctga
     2041 gtccttttca gaaactaaat taaatctagt gtttacaaca aagttttcat gttctatatt
     2101 gtttagatta tttgcggaaa ggagggcttc tagatcttga cattcaaatc tatatgcatc
     2161 gtcataaatc ttactagaag gagtgcttaa ttcgaaatta cttattttct catcttggac
     2221 caagataaat ttatagatag tatctttttg tgtttttctt ttaaaaacac aacagagtgt
     2281 gttataagga acgttataat tttcatcagc ctcatttttt ctccattcaa acaaatgata
     2341 aactgcaacc aagttgccat caatttcatg gacagtgctc tcggtaacat cctttaaaga
     2401 actatcaagg gctaatttat agtaactaga gagaggaagt aaaattgtcg atttactgag
     2461 atggttatcg tctatacgtg caatcatacc taaatcaact aaggttttat tccaggtgtt
     2521 tatagtttta gttgtcactc ctagagactc tgctatggta ttgaccgaat gccaggaatc
     2581 cccgccttca ttctttgcct taaaacagta atacagatat aaattaatag ctttgttatt
     2641 cggtaaatat ttttcaagtt ctgttggtag aataaagtaa gaatccttaa tgttttttct
     2701 gaactgtctc cagtctctgt agttttgttt ataaagttgt gaaacggttt ttccaccttt
     2761 agaaagtcct gctatcttaa ccaatcttat tgctcctttc ttgaatctgt catgatcata
     2821 cagtattttt tacttgtagt ccagcacctt aatatattat cctatgtaat taatttcgta
     2881 ctagatagta attatttttt atttaattct ctagtcactt acaaataagt tatataaact
     2941 acgattaata aattattaca aaaactatta agaaaacttc tacatacaac tattacaaaa
     3001 ctattaagaa actgaatact cctatttttt gtttttaagg aaagtttttt cttttttgaa
     3061 tttcttattt ttttctaata taactaagca aaactggata atacgacact tattttcaaa
     3121 ttttcacaag actaatagta aatatccatt taataaagtg atacaatgtg ttgattttac
     3181 aaatcaacag aagaagtagg gaaataactt acctatgctt ctttttttgt acatttaatc
     3241 gattcttctt tacaaaaacg aacgtatgtt tgtataatgt ggaagaatgg agtgttattt
     3301 atgatggatt attcaaaaga gccagtaaat gattactttt tgatcgatat gaaaagcttt
     3361 tacgcaagtg tcgaatgtat agaaaggaat ttagatccat taacgactga attagtcgtc
     3421 atgagtcgag cggataatac aggatcaggg ttgattttag cttcttcacc agctgcaaag
     3481 aagaagtacg gtatttctaa tgtcagtcga ccacgagatc taccgcaacc ttttcctaaa
     3541 acgttacatg tcgttccacc acgcatgaat ctctatatta agcgaaatat gcaggtaaac
     3601 aatattttca gaaggtatgt agcagacgaa gatttattga tttactcgat cgatgaatcg
     3661 atcttaaaag tgaccagatc aatgaatctt tttacgactg aaggaacaag aagtcaacgg
     3721 agaaagaagc tggctcaaat gatccaggag aatattaaag aagagctagg attgcttgct
     3781 acagtaggtg tcggagataa tcccttatta gctaaacttg ctttagacaa tgaagcaaaa
     3841 cataatgatg gattccttgc tgaatggacg tatcaaaatg ttcctgataa agtatggaat
     3901 attcctgaaa tgacagacct ttggggtatt ggatctcgaa tgaaaaaaag actcaaccaa
     3961 attggtatca tgagtattaa agacttagct aattggaatc catacactat taagagccat
     4021 ttaggtgtga ttggcttaca gctgtatttt catgcgaacg ggatcgatcg aacagatata
     4081 gcgatcccac cagaaccgac aaaggaaaaa tcatatggta attcacaagt gttaccacga
     4141 gattatacca ggagaaatga aatcgaactg gtggtaaaag aaatggcgga acaagtagct
     4201 attcgtatca ggcaacataa ctgtaaaaca ggttgtgtcc atttatacat tggcacgtcg
     4261 atcttagaaa cgagaccagg tttttcacac cagatgaaga tccctataac agataataca
     4321 aaggaattgc agaattactg tcttttcttg tttgataagt attatgaagg acaagaagta
     4381 aggcacgttg gaataaccta tagcaagctt gtgtacactg attcgcttca gctggattta
     4441 tttagtgatc cacaaaagca aattgacgaa gaaaatttag ataaaattat tgacaagatt
     4501 cggcaaaaat atggttttac gtcgattgta catgcttctt ctatgttaga aggcgctcgt
     4561 agtattacta gaagtacatt ggttggtggc catgcaggcg gcgcaggagg gcttaaaaat
     4621 gattgattat aatgatcgtg gacgtttaaa gtgggcaggt ttttatttat ctgatcatac
     4681 tgaaaaaatt gatgccgaca atgaacaacg agccaatcaa aatttggcaa aagaacaaat
     4741 gacgactgaa gaggttgcgg aagtattgaa cgctgcagtc ataaaaaata gatcagtatg
     4801 tattcaaaaa gaagaacgaa acgctgaagg atactatcca gaagatattg ttagcaagat
     4861 cttaggtagt gatgagctgg ggatctacgt tggaaaaaac aaagttcact acgacgaaat
     4921 acgtcatatc gaattttacc aggataataa atggggtcga ttagaatgaa cattatccag
     4981 caatatgagc taaagtatat aacctttgat cagctatcag aagagatttg gggttatggc
     5041 cagcgtttga taaatgaggt tggtgttgaa cgcttctctt tctatgtaga agctgcagca
     5101 ggataccata aatacgaata ctatattggc tattaaagtg caataaaatt atatttcttt
     5161 tatgttttac attttgtatc aattctgata aaattaccta taggtaatta aaaaatataa
     5221 aaaaactagt tacaacctta ggacgttgta actagcttga agcatactag agctaactaa
     5281 aatagcacta gaaagcaatg ttaaacccgt ccaacaaact tgatttaaca ttgagaccag
     5341 cttattcagc taaatatagt tgaataagct tcttttgttt taaaaaaaca aaaggtgtac
     5401 tacgaataac gcaagtaaaa gaaatactat tacaaccagt aggctacaag taagtattac
     5461 taaacattgc gtagttagaa cctttgctct attgtctaaa gccacaaaat tagagatcag
     5521 ctttatgatg ctaagagcga gctgcatcca tgcggacata ggttcttttg acactattaa
     5581 ttctcctttt taggataagg gaaattgcta gtagtttttt cattagcacc atcccccttc
     5641 atttgattga gtctagtgct gctgtgatag tttgctgttt aatatgctta cattcttatt
     5701 ataacataaa tgaaacaaac acatggttag aggtcgtatc aatcaaaata gtgtctcaca
     5761 aacgatttat tacttactcc tgctgactaa cggactgttc ttcagtaact gtaactatat
     5821 gtttgttaaa accaccatta cctgtagata aaaaccaata tatttactat actcactata
     5881 ttaaatatat ttagtatagt aaaaactaga aacaataaaa tttgtgaatg ttgtataccc
     5941 ccaaacaata cacatatttt gaaggattag gattaataaa gctaaaaata tagtaagtat
     6001 actaaatata tttactatat ctagtatact tactatattt taatcttttg atcgtctatt
     6061 atatataatc cgttcctcgt cgtttaaagt atcaacatag gcgtccattg caagtttgac
     6121 tatttcatac ataggaatat cttttgcata actaagatcc cgaatggttt ttagtacggg
     6181 aggatctact tgaactggtt ttctttcttt cgcagtgtac tttcgttccc ctaactcgac
     6241 attaatatca tctttaattg aaatatcttc agcaccaatt ttcttcttat ctttgttcgt
     6301 tggtaataat gccatttagt ttaatctcct ttccaagagg taggatatga ttggccattg
     6361 agtcagcata tttaggtgta taatcaaatc cttcaatatc accatgttgt tcatagtatt
     6421 caattctttc ttctaattct gtaaagatgt ctgcaaaaat agcccaccat cttcgatcta
     6481 cataatcatt taattggata ccattctcac catacatttc taaccgatcc gagcctttaa
     6541 caatgctttt aaaaatgttt gattcaccat acttatcaat tgttttttga tagtttgcgt
     6601 gttgagagga acgtcgcact gagaaaagaa caggcaaaat tccaatgatt tgaagatgag
     6661 actcttcaaa attcgtgaca atcggaacta aaactttatt aataaaatct tctgttcctt
     6721 ccattgcgta ccgttttaat tcttgcattg caataatata atctgcagca gttaagaatg
     6781 cacgaaccac attatctgtt gatggtgggc agtcgataaa gatataatcg aaattttttc
     6841 tcaattcttc taaaggttta atgaaggcca atgatctctt acgacgatca ggatattttt
     6901 cggacaagta ttcattaagt tcttcagatc caggagagcc agccataata tataaatgtg
     6961 gacttacttg agtaatacct gaaacccagt cccctttttt gattgctgca gacatggatc
     7021 gatcaaagct agtaatttga tacgttgatc caatgttgga agtagtattt ctttgttggt
     7081 cataatcaat tagcaacacg tctttttcaa aaagttgact agaaacaatc gctaataaat
     7141 cattgtctgt agtcttacca atgccgcctt ttctacctgt aagcattaca acttttccta
     7201 gtttatctga tgttttaaat gcagtccgta agttttccat agttccctcc aatttcctat
     7261 actaaatata tttagtatac ttcatatact taatatattc gatatatcta atatactcca
     7321 aattgataag aattcagact ttataaggaa tatagtggat atactaaata tatttgctat
     7381 attcagtata tcagatatat tcaaaatgtc aaaatatatt tgtgcgtttt gcgttgacaa
     7441 tttagagtga aaagaggtaa acttaattct atagaaacca cagataattc actatttaca
     7501 aagtagcaaa agcagtggta ctctttaaac agaaaaatta cataaaaaaa agagacggaa
     7561 ataattgatt ttagcagaat caattatttc tgaatcacat ggttaggaca tgtgttcacg
     7621 ctcgtctcaa gaatatgtaa gtagctacca actacttata ttaaaagttg atcggatacc
     7681 ttttccgatt gcctttattc taccaaaaaa aggcgagcaa ctcaacagaa gaacgtttat
     7741 ttagtgtact taaattaaat attcttaaac actgaagaga gaacacatac tatggctgag
     7801 attaaagcta aactgtgttc tctttttgtt tgagaaagga cgttacaatc gatgttgagc
     7861 gaaatttatt cagctattct taaaaacggc ttacgtacat acaaatatcg aaacagcacc
     7921 atgaaacctg caggtcatat tgattccagt aggagtggtg ctatctttgc gtaccgtagc
     7981 aagcaattaa tgaatgttgg gcgtggtatg gtcattactt cggaagaagc tattttagag
     8041 aacgaaaaga aactaacgca ttggacacct aatacctatc gctttggtac ttatgcggat
     8101 gattatcgaa cagtggttaa aggtcattct gagaaaaact taagtcaaat caacacattt
     8161 gtagttgata tcgattcaaa agagaaccat caaggcgaaa ttattttagc ctgtttagat
     8221 caagtgggct acatgccaac gctaatttta gaatcagatc atggttatca agtttatttt
     8281 gtactaaaga caccaggcta tgtcacaaag aaaagcaatt tcaaggtgat cgatgtagca
     8341 aaaaaaattt caaaaacaat tcgtctacag ttggctgaaa aattgtccgg agtggatcta
     8401 ggatgtaacc acttcggaat tgctcgattc ccacaagaaa ataatattgt ttttaaagaa
     8461 atgaactatc agtattcttt tgctgactgg attcaatggt cgatgaagct tgaatcagat
     8521 caaaaaacaa aagaagatcg agaacgaaaa ctaattgtct ttcctgagaa aaaagaaatt
     8581 cgtcaagtag atgaaccgtg gttcgatatg ttacttcata aagcaaacat cgtcggcgga
     8641 gaaggacgtc ttggtcgaaa taatgtgatt ttcacgcttt ctttagctta ttactcttca
     8701 ggttatgcat tggatacttg cgaatacaat atgttcacat tcaacgaacg gttacaggaa
     8761 ccgctatctg aagcagaatt agtaaagatt gtacgtagtg cttattctgg taattatcaa
     8821 gcagcaagca gagaattcat tattgaattg tgccagcaat gggtttcacc aaccttaaaa
     8881 aataatgatc tgttcattca acgacgtggc tggtggaaat ttaaaaagcc acgagatcaa
     8941 agaaaatata gtcataaaaa tgaatggaaa gaagatctat tggcttatct aagcgaaaaa
     9001 agtcttgcta gtgagcctta tttgaacctg tcaaaaaaag aattggcttc acaactaacg
     9061 atgcccaagc gaacacttga taagatctta aatgagttac gagaggagaa taaagtcttc
     9121 tacaaggtaa cacgaggaag aaatggcgga atgatcattg cttcagtcaa aacgttgttc
     9181 gcgagggtta ttcaactaag taaagaaaag agactagatt attcaacaag gatcacagag
     9241 gtatttggac tcaacgagag cttagtagaa gagacattta gacaattatc aacccccttg
     9301 aaccctctta aagaagtgaa tctttttgaa gtagatacag gttgaaaaat aaaaggtgca
     9361 aattgcccta tacaatatat ctatgatact tctgtaggtg ttgctccctt tgcctagcta
     9421 cgtgtaggtg gtatacttat aactgcgtat gcagtcttat gtatcatgga ggaagttgta
     9481 tgtcgttaaa aaattatgtc tattcacatc ctgttaatgt gtttatcatt ggtcgtttgg
     9541 gattatctgt ggaagtgttc tgtgagcttt atggttttaa gcaggggact ttgtcttctt
     9601 gggttaccag ggaaaaaaca gttgcttctt tgccgattga gtttcttcat gggttgtcct
     9661 tagcttctgg ggagactatg gatcaggtat atgattgttt gtgtgtgtta gagcaggagt
     9721 atattgagta taaaatagct aacgagttaa gaaaaaggaa gaagtatatc cagtagtagt
     9781 ttttatttac ttaaaaaatt ggttcattga gcaacctatc gctgctaaag tagcaaagga
     9841 aaacgttaaa ggaaagaatt tagggaagaa atcgaaatga aatcggacga ctggattgaa
     9901 cacacctggc ttcctctgaa aagaaatagc aaacgaaaga aattttgggt attgcaatta
     9961 tggccattag gaaaactttg tagttacggg gcacgactta ttcttgattt aaccgtttac
    10021 tcgattggag tatttggaac ggcttattac ttgataaatg atcccaatag gattgcagca
    10081 tcagacattt ttacagtgct aatgacgttg atttttgtca tgctattgtc tatttttact
    10141 caattagtca tgcaatttac caggcaaaaa ttaacagcag gaaaactttt caaaagccta
    10201 ttcgatttaa ttgttgtaaa tgcaattcta gcgactttaa taaaacaaat tgaacaatct
    10261 ggaagaacaa caaaaggagt gaaggatttt ttattgacgt ttattcactt tgtaaatgga
    10321 cacatgtatt tgtttgaagt gggtattatt ctcttttttg tcgggtatgt atccacgaaa
    10381 cttattaaac gttcaattga aaaagaaatt gaaattgacc tgattccgac acttcttggt
    10441 tatgagtaca atgttgattt tggaagatat gacgaattga tcaatacgga tatcgttcac
    10501 acgagtatcc atgaatctac tgttaagggc atcagatcta ttttaaaaaa tggccaggaa
    10561 atgaaagcaa tagatcgaag aaaagagatc tacaattttt atgaagtcga agcaagccta
    10621 cgatatggca tcggaaaatt gtggttagat ggaagtaata ttcgtcaaaa caaaccatac
    10681 attttttgga acagtagtga tttagaactt ccaatgaaag ataaataatt atggagaatc
    10741 gtttgaaaat gattcctcta tacaggattg tgcttatagt tggcatggag ttaaaaacct
    10801 taaagaagga gcagttattc aaagtgaaag aacgtcataa tcagttacta tgtagtaaaa
    10861 cgggtacgat gaacattgaa taccgaacag aatatttaga attaacgaat caaactgatt
    10921 ttttgttgat tcgaaagcaa caagagcaag aaaaaaatta atggctactt atagccaatt
    10981 gattgatgaa tgggaacaat tcaaggaatc taaaattcca gaacgtctct aaaagccttt
    11041 atttgtaaag agcataagga aacgtgggaa cactttttaa aaaaagtgtt ccaataggct
    11101 gtgcctatta cgtttctcaa aaataaatgt gggaacaaaa tatttaaaaa taggatagtg
    11161 aaaggatagt gaaggatagt aaaaggaggg tagtaggata gcaaaaagga taggctcctt
    11221 ttcgctatcc tttttaaatt ggaggaatta atatggaagt aagaataaga aatgtggatg
    11281 agaaaatcgt aagagaaatt gatcgaagat gtgac
//
LOCUS       DAMEET010000080        11244 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.081, whole genome shotgun sequence.
ACCESSION   DAMEET010000080 DAMEET010000000
VERSION     DAMEET010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.081"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(7..1236)
                     /gene="hflX"
                     /locus_tag="PB780_002055"
     CDS             complement(7..1236)
                     /gene="hflX"
                     /locus_tag="PB780_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDE0734099.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1238..2155)
                     /gene="miaA"
                     /locus_tag="PB780_002056"
     CDS             complement(1238..2155)
                     /gene="miaA"
                     /locus_tag="PB780_002056"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDE0734100.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2159..2896)
                     /locus_tag="PB780_002057"
     CDS             complement(2159..2896)
                     /locus_tag="PB780_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDE0734101.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2987..3514)
                     /locus_tag="PB780_002058"
     CDS             complement(2987..3514)
                     /locus_tag="PB780_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734102.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3694..4287
                     /gene="clpP"
                     /locus_tag="PB780_002059"
     CDS             3694..4287
                     /gene="clpP"
                     /locus_tag="PB780_002059"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDE0734103.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4391..4876)
                     /locus_tag="PB780_002060"
     CDS             complement(4391..4876)
                     /locus_tag="PB780_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDE0734104.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5029..5226)
                     /locus_tag="PB780_002061"
     CDS             complement(5029..5226)
                     /locus_tag="PB780_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734105.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5321..7081)
                     /gene="efrB"
                     /locus_tag="PB780_002062"
     CDS             complement(5321..7081)
                     /gene="efrB"
                     /locus_tag="PB780_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HDE0734106.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7078..8808)
                     /gene="efrA"
                     /locus_tag="PB780_002063"
     CDS             complement(7078..8808)
                     /gene="efrA"
                     /locus_tag="PB780_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HDE0734107.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9201..9413
                     /locus_tag="PB780_002064"
     CDS             9201..9413
                     /locus_tag="PB780_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HDE0734108.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9415..11094
                     /locus_tag="PB780_002065"
     CDS             9415..11094
                     /locus_tag="PB780_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDE0734109.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
BASE COUNT         3258 a         2429 c         1971 g         3586 t
ORIGIN      
        1 aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc ctttaacttc
       61 atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc ttctcaactc
      121 gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca ttagttccat
      181 cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct gagtagaaat
      241 caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt ctgccttatt
      301 gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt ttaagacagt
      361 ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca aaagatccat
      421 attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag gcaggtcttg
      481 gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac gccatctctt
      541 agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg cttgtgtcaa
      601 tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa tctgaaaaat
      661 ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt ctttcaattc
      721 tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta actttgtttc
      781 acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc cttgccctac
      841 taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc cttctttcga
      901 acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta ccggaagacc
      961 aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa agatgatcaa
     1021 atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt taccgataat
     1081 cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt ctcctgatgc
     1141 ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct ggtttgcttc
     1201 ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc agccattttt
     1261 tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca aaccactgtg
     1321 catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc gaatgtagtt
     1381 tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg aaaaattctt
     1441 tatatccaat accttgactg gcctggactt ctccaagctc atacacctta cgcgcttctg
     1501 ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca taaagaatac
     1561 tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc tcttctttag
     1621 gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc actttgcgtt
     1681 gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac tgattccata
     1741 atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc tcaggattcg
     1801 cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag agacccgtac
     1861 caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat tgtcgacctg
     1921 aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca atcaaatgat
     1981 gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc agatttttat
     2041 agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg agttccacac
     2101 ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt tccatatttt
     2161 acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca gttattattc
     2221 catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc gtccatgcac
     2281 gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa gtattttttg
     2341 gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta tcagaagtac
     2401 tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc aaagaatcca
     2461 aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat gtttcggcta
     2521 atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc attagcatcc
     2581 ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga actttagctg
     2641 ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct tccaatgttt
     2701 tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg tgcataacga
     2761 tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca cctactcgta
     2821 cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt ccgcgatggg
     2881 catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt aagaaaaaaa
     2941 gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag ctccaaacat
     3001 catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga gctttttttt
     3061 cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt ccgcctttat
     3121 aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt cgatgggaga
     3181 tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt tgcgctgaaa
     3241 atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat gcatcaaagc
     3301 tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc tttttattta
     3361 attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta cgctgttctt
     3421 tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca gcacctgtca
     3481 gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt gttttgtttc
     3541 tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt ttttatttga
     3601 ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat tatggtacac
     3661 tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac agtcattgaa
     3721 caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa agaccgtatc
     3781 atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc ccaattgctt
     3841 ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc tcctggtggt
     3901 tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc agatgtccaa
     3961 acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc tggtacaaaa
     4021 ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct tggcggcgct
     4081 caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac acgcgaacgc
     4141 ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga aaaagataca
     4201 gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat cgatgaagtc
     4261 atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt aagctctctt
     4321 atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt tattttatgg
     4381 atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga cctatctcga
     4441 cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa ggacggatca
     4501 ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct tctgttacta
     4561 atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat atcgtggtcc
     4621 ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac tgatgtccta
     4681 ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg attgtaccat
     4741 cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt aaaacagctt
     4801 gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg gcatttgatg
     4861 ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact aatcctattg
     4921 aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa aacctgctca
     4981 ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt attgtacatc
     5041 tatattttct tctattactt tttttaaaat taattcgtac acttctattt gtgctgccaa
     5101 gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct tcgtctgaca
     5161 gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat ttaaaaactg
     5221 ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc aagaatagag
     5281 aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct tcctcagcaa
     5341 attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc tcatgcgttc
     5401 ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca cggattgtcg
     5461 ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc attgcttttt
     5521 gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa atcaagattt
     5581 taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct aaagacacat
     5641 tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg ataaaatgat
     5701 ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca agctttccga
     5761 agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag accataccaa
     5821 atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca atccttattg
     5881 ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc ttgccggcac
     5941 cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc aaatcttgga
     6001 ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca tctcctaaaa
     6061 tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca tctaaaatct
     6121 cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat tgtgtcacgt
     6181 tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt tgaccgacaa
     6241 cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata taagtagcat
     6301 aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca cgaaacccaa
     6361 agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt tcgcgtccat
     6421 atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt agctcaccaa
     6481 gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt tttgagatcc
     6541 agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg atataaaaca
     6601 tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata aaagcttgtt
     6661 gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata tttccttgct
     6721 ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg tctcttctca
     6781 aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt aattgcgaaa
     6841 tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata taagaaaaat
     6901 tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt cctgtaacat
     6961 atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact aaaagaaaag
     7021 tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag gtttttttca
     7081 tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg ggcatttttc
     7141 caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata cgatgatctt
     7201 atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag attctgtcgt
     7261 ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg cagaaaagct
     7321 gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg ccaatcgttg
     7381 tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa atccatctgg
     7441 ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca tttcttcttg
     7501 cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa atagcagcgc
     7561 tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat ctctcacgtc
     7621 tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga tcaattggat
     7681 caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc ctggagaggc
     7741 tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag caaatgttac
     7801 attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg gatcttctac
     7861 aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag ctgctcttgg
     7921 atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga atatatactc
     7981 gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca ctgccccgct
     8041 ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt gtgcaacagc
     8101 catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta cagcaaaacg
     8161 tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg aaagattttc
     8221 ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata acggttcaga
     8281 caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta ctacatacaa
     8341 actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca tcattggtgc
     8401 cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag catcgtttgt
     8461 tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg aataactttg
     8521 gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa tcctcgaacc
     8581 aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga tcataaatat
     8641 cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga tcatacgtgc
     8701 taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata tgaaattgaa
     8761 aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt tctcatctcc
     8821 ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc ctattaatag
     8881 aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca ttgatttttt
     8941 ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata acaattaatt
     9001 gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga taagcaaaaa
     9061 cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga aaaaccaggt
     9121 ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca ctgctttacg
     9181 cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca aaagtacgca
     9241 accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat gtagacgctc
     9301 gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt ctagaaggaa
     9361 gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt taatatgaaa
     9421 ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg tgaaatcggg
     9481 aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc cgggatcaaa
     9541 ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag ctacatcata
     9601 caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga ccacatcggc
     9661 ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc tctcgcatta
     9721 gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga gctccatgag
     9781 atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt cagaacgacc
     9841 catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa tatcgtagcg
     9901 actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt gcataaaatg
     9961 gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa tgctgagatt
    10021 ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat cttttcaaaa
    10081 atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt gcagcaagca
    10141 gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag catggaaaat
    10201 gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac atttgtagat
    10261 gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac aggttctcaa
    10321 ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca aatccaaatc
    10381 cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac aacaagtgtc
    10441 aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg aaaaatcaat
    10501 aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct tcgtttgatg
    10561 aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt acatgcttct
    10621 ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa tggcgatgtt
    10681 ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg agatgtgtat
    10741 atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg tcacctgctt
    10801 tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa agaaatcttg
    10861 gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg agaaatgatc
    10921 aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg acctgactgt
    10981 tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt attcgaacgt
    11041 acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga ataaataaga
    11101 ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa acatgagaag
    11161 ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc tcttaaattt
    11221 atttactttt tcatacaaaa aaat
//
LOCUS       DAMEET010000081        11150 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.082, whole genome shotgun sequence.
ACCESSION   DAMEET010000081 DAMEET010000000
VERSION     DAMEET010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.082"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            6..305
                     /locus_tag="PB780_002066"
     CDS             6..305
                     /locus_tag="PB780_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDE0734110.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="PB780_002067"
     CDS             378..1739
                     /locus_tag="PB780_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDE0734111.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFLSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLLFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGILTMPIYLYGATYLAPILSDSAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="PB780_002068"
     CDS             1761..2021
                     /locus_tag="PB780_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734112.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="PB780_002069"
     CDS             2035..2787
                     /locus_tag="PB780_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDE0734113.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="PB780_002070"
     CDS             complement(2881..3486)
                     /locus_tag="PB780_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDE0734114.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIEQAREWTDHLLTK"
     gene            complement(3542..4516)
                     /locus_tag="PB780_002071"
     CDS             complement(3542..4516)
                     /locus_tag="PB780_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HDE0734115.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DCPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4894..5613)
                     /locus_tag="PB780_002072"
     CDS             complement(4894..5613)
                     /locus_tag="PB780_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDE0734116.1"
                     /translation="MPKYEEIANILRERIRSKEYPPDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5740..7011)
                     /gene="celB"
                     /locus_tag="PB780_002073"
     CDS             complement(5740..7011)
                     /gene="celB"
                     /locus_tag="PB780_002073"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDE0734117.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7164..8561
                     /locus_tag="PB780_002074"
     CDS             7164..8561
                     /locus_tag="PB780_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDE0734118.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWNHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSRLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKAIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGTIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8642..9082
                     /locus_tag="PB780_002075"
     CDS             8642..9082
                     /locus_tag="PB780_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDE0734119.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9229..9555
                     /locus_tag="PB780_002076"
     CDS             9229..9555
                     /locus_tag="PB780_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDE0734120.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9556..10305
                     /locus_tag="PB780_002077"
     CDS             9556..10305
                     /locus_tag="PB780_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734121.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10331..10741)
                     /locus_tag="PB780_002078"
     CDS             complement(10331..10741)
                     /locus_tag="PB780_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HDE0734122.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNK"
BASE COUNT         3538 a         1948 c         2291 g         3373 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttcgaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcctgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtctta ttgctgcttt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattacca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcctc acgatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgattcagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaaggaccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcaaaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgggagat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc tcgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccaatc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa aaatgaagta gcatgaagag
     3541 attattcacg ttttttattt gcatcacttg tatcgaaagg acggacatag gcttcaatgg
     3601 cttcacgacg ttcttctaag aatggaggaa gagatagttt ttctcctgct gattctgctg
     3661 gttcatcttc aaggaatcca ggacaatcag ttgctaattc aaacagtacg tgatgagcgg
     3721 ctaggaagta ctcagattgg aaatagaaac gatcgacgaa accagaattt ggcaattcaa
     3781 tcttattgat tacttgaatc cagtattcca atgcttcttt gtctgctaca cgcaatgcta
     3841 agtgatggat atttccaaat ccttcgattg cttttggcaa gtctgtccga tgttcaataa
     3901 tgatgcttgc accatttccg ccttcaccga tttcaaataa gtagaaatct cctgaatggt
     3961 ctgtttcttt gaatcctaag attttttcaa gaatcaagcg gatatgctct aattgactga
     4021 cacggacaaa gacaggtcca agtcctgtga tcgcaaactc tgtcggtaca ttgctgtttt
     4081 tccaaggaat gccagctgct acaccttgat tgttttcgtc agagatcaat tgataatact
     4141 gattatcgaa atcttcaaat tcgatatatt ttttgccgaa acgttcattg attgctgaat
     4201 gagtaacgtc atatttttca aaacgttcca accaaaagtc taatgctgcg tcacttggca
     4261 cacggaaaga agcacgagag atcgtatctg ttcctttttt tccttttggc tgatttggaa
     4321 aatcaaagaa agtcatatct gttcctggcg caccagtatc gtctgtaaag taaagatggt
     4381 aagtttcgat gtcatcttgg ttaactgttt ttttgattaa gcgcattcct aaaatatctg
     4441 taaaaaagcg gtagattttt gtagcgctag atgtcattgc tgtgacgtgg tgaattcctc
     4501 tgatttgtgt atccatgata aatttcctcc tgtcaactta caaaaagtaa gtagttgatt
     4561 tagacatagt atagagggtg catctctatt ttgcaattaa tttgtcttct aaagtatcag
     4621 gaaggaatca agaatggaaa agaggaggga cggatcaata aaataaaaag acttacgact
     4681 agctgtatcg atttcaaaaa aactagcttt aaaaagctaa ctttagaatc attacgggtg
     4741 gtcataagtc tttttatgct gagttatttt ttcatttttg agctaatgct taggatagga
     4801 tagaatgttg atttcacgat ctcttcttgt tacttgaaac tgcttactac tgtcacgatc
     4861 tcttggtaaa cggctttttc acaacctctt catttaagtt gaacgcacat ccagcacaga
     4921 atagttccga acatcaaatc ggtttcggct ggaagaatat tcaatcggct gtccatcttt
     4981 gagatagacg acttgctgaa cttccagtac aggatcgttt tctgcacagt tcaaatattg
     5041 ctgatcatat tcatcacttt tatctgcaga aatagcacga taagcccctg caaaatggac
     5101 attcaattct tgatgaagat gatcatagat agaggctaac aaatgttctt tttttaaacc
     5161 cggcacaaga tggactggca taaaagtatg ctctaaaaca tatggctcgc catctaagat
     5221 ccttaaccgg tgaatattgt agacaggttg ctcggcatcg atcatcaatc gttcttggat
     5281 atattgatta gggaattcaa catcaaaatg aatgatttga ctcttcaaag aacgatgtct
     5341 gcgtttcatt tggagactca aaccttcata ctcgttagcg ggcgatgtgt ctttttgcat
     5401 aaatggatga ttcaagattt tcgttcctga tcctcgctgc gaatagacaa gtccttccat
     5461 tgctaagata ttgatggctt ttttgattgt catccggctc gcaccaaatt ctttgaccaa
     5521 gtccacttga ttaggcaaaa aactatcggg aggatactct tttgaacgga tacgttctct
     5581 taaaatattt gcgatttctt cgtattttgg cacgttattc acctcttcta gagtataacg
     5641 aaagattatg cttccgacaa ctcgaagtag catctggaac attgtttatt cgaaaaaaga
     5701 ggtcgtgaca agtttttgtc acaacccctt aatgatctat taagcaatat ctaaatctgg
     5761 ttgtttatct agtactttca ggaatgggta ccagataaag gcaacgatga caaagtcgat
     5821 gacttgcagc ataccaccaa gccatgaatt tgttgctaag acaccgcttg caatgattgg
     5881 cacagtccat ggaacagaga cgccagttgg tgcagggaca agtccagcag ccattaccaa
     5941 ataattgaac gtcgttacga ttagaggtgc cacaacccaa ggaataagga ttgttgcatt
     6001 caatacgatc ggtagaccga agatcgctgg ttcatttaca ttgaaaatac caggtgctaa
     6061 agctaatcgt ccaacatcac gatactgttt ctttttaaga acaaaggcca tcaataaaac
     6121 gacagctaaa gtcattccag agccaccaat accaacagtg aaggtttcca tgaatggttt
     6181 tgtgatgatg tgtggtaatt cttttccatg ttgataagct tctaagttat caagcatcag
     6241 tgtattccaa atcgggtcca ttacagagtt gacgatgatt tgtccgtgaa gtccgaagaa
     6301 ccataagaat tggacaaaga acaaagcaat caatgttgca ggtaaactac ttcccaaacc
     6361 agttaatggt ttttggatca cttcataaac gacgtcatgt aagttagcat tgaaaatacc
     6421 agtcatcaca gcattgatta ctaagaaaat agttagtgtc actacagcag ggatcaaagc
     6481 ggcaaatgat tttgttactg caggcggaac tccatcaggc attttgatct gccagccgcg
     6541 tttcgtgatt ctgcagtaga tttctccagc caagaaagca gcgatcattc caatgaacat
     6601 tccttttgcg ccaagacgat caagtgaaag cacaccagtc acatcttcac cgccagcggt
     6661 tttcattgca aaaggcgtaa gaatcaagaa gctggcaaaa gacacggcac caccgaaaat
     6721 accttcaacg tcataagatt ttgataaata atagccgata ccaaaagtaa caaaaaccga
     6781 catgatgctc attgttgcat tttgcccatt accgaataag ttcgataaag ttccctttgt
     6841 tgcgtcactg aaaaaaggca agttattgat aacgacaacg atcgaaccga acatcgttaa
     6901 agggaaggaa agcatgaaag catcccgcag cactgtaagg taacgatttt gccctaataa
     6961 attagctaag ggcatgattt tctcagaaag cttgtccata aaaccgttca tttttattca
     7021 cctcaaattt atttgcaatc gattacatac aaagtataac gccaaaaatt ttaaatgtct
     7081 agtcattttc ataaaaagta ttgatttatt ttttagggtt cgttatgatt acgatatcaa
     7141 gaaaaaagag aggaagtaaa aaaatgaaaa cctacacatt cccagaaaat ttttggtggg
     7201 gttctgctgc tagcggaccg caaacagaag gaagagtaag tgatgatggt aaaggagaaa
     7261 atatttggaa tcattggtat gaattagagc ctgaaaaatt cttcgatcaa gtaggacctg
     7321 aaaaaacttc gcaagtttac acaaaataca aagaagatgt tcagttaatg aaagaaactg
     7381 gccataacac atttcgtaca tctatccaat ggagtcgttt gattccagaa gggacaggtg
     7441 aaatcaatca aaaagctgtt tctttttatc gagattattt tactgaattg ctggataatg
     7501 gtatcgaacc ttttgtgaac ctttaccatt tcgatatgcc actaccactg caagaaaaag
     7561 gcgggtggtt atcaagagaa acggtcgacg cttatgtttc ttatgcagca gcgtgctttg
     7621 aactgtttgg agatctggtc aaaaaatggt ttactcataa tgaaccaatc gtacctgtag
     7681 aaggaggcta cttgtatcaa ttccactatc caaatcatat caatatgaaa gaagcggtcc
     7741 aagtaggcta tcatgagaat ctagcaagcg caaaggcaat caaagcctac catgaaggcg
     7801 gacatgatgg tgagatcgga atcatcttga atctgacacc aagctatccg agagatgaaa
     7861 cgaacccaga agatgtaaaa gcggcgaaga tcgctgacgc attcttcaac cgatcattcc
     7921 ttgaccctgc agtgaaagga gagtttccac aagagttgat cgaaattgtc aaagaattgg
     7981 ctataatgcc agagatccat gaaggagacc tagaactcat ccgtgaaaac acgattgacc
     8041 tgctaggaat caattactat cagcctcgtc gaatcaaagc aaaagaaact ccaatcaatc
     8101 acgaacatag ccccatgcca gatgattatt ttgataatta cgacatgccc aataaaaaaa
     8161 tgaatccata tcgtggatgg gaaatttacg aaaaaggaat ctatgatatt ttaacgaata
     8221 caagagaaaa ctatggtaat atcaaatgct atatttctga aaatgggatg ggggtcgaag
     8281 gagaagaacg atttgttaac agcgaaggaa ctattgaaga tgattaccgg attgagtttg
     8341 ttacggatca tttgaaatac gtccatcaag caatccaaga aggaaccaat tgtgtgggtt
     8401 accacatgtg gacatgtatg gacaactggt cttggacaaa tgcttataag aatcgctatg
     8461 gctttatcgc tgttgatttg gcacaagacg gtaaacggac gatcaaaaaa tcaggtcgct
     8521 ggtttaaaac ggtctctgaa aaaaacggat ttgatgcctg atattttttg ataaaacgtt
     8581 cacaaaatgt ttgttctaac actgtaatcg tgctacacta acagtgaagg aggcgtttac
     8641 aatggtagat acttatcgta atattttagt agcaattgat ggttctgaaa aggcagaaaa
     8701 agcatttctt gaggcaatca cgattgccaa acgaaatcag gcaacgttgc acatcctgta
     8761 tgtcaatgaa gtaacaggca attactttgg tgattttgca tttgttacaa caagccttca
     8821 ggaagaattg gatgaggttg cggaaaacca gatgaaagaa catcgaaact tggcaattga
     8881 aaaaggcttg acagatatcg agacatatgt tctttacggt tatccaaaga cactgattgc
     8941 taatttccat gaatcgaaag aaccaatcga tctgattgtc atgggggcga caggtctgaa
     9001 tgcggtagaa cgtgcactag taggttccac tacttcctat gtagtcaacc acgcatcatg
     9061 taacgtcatg gttgtaaaat aaataaatta agggaacaga aagcaaaaaa gcataacgct
     9121 tcttttgctt tctgttcctt taattttttg taaaaaatta ctttacctga tgaagtaatc
     9181 atgctattct aattacaacg acagatggag taatatggag gaaagaaaat gatttcaagt
     9241 gatggaatac gcggatacaa tgatgcgatt attttatcga tccttaacaa aaacgattct
     9301 tatggatatg aaatttctaa aacgattcgt gagaggacaa atgattccta tgtgatcaaa
     9361 gagacaacac tttactccgt ttttgcccgt ttagaaaaga atcaactgat tgtttcgtat
     9421 cctggagaag tgactcatgg aaaaaagcgg acatattata aaatcactga aatagggcgt
     9481 aatcttttag aagaaaaaaa gcaagagtgg ctgacgacga aagacgtagt cgatcaattt
     9541 ttggagggaa attaaatgga tacaatccgt aattatttag acagtctttt tatcggagta
     9601 cctcaaagta ccgagataga taagctgaaa actgatttgt tggcaaatat ggaagaccat
     9661 tatcatgaat tgatgggaga aggaaaaaat gaacaggagg ccatcggtac agtaatttcg
     9721 acatttggtt caatcgatga attattagaa gaactagatg tcgaaaaaaa acatcaagct
     9781 gacgagacgg aaacaaacac agcttctatt tatttaagcg aagcagaaaa ctactggaaa
     9841 gaatacagag cggcaagtct ccaagtggca tcaggtgttc tattcattag cttatctttt
     9901 gcaagttttc ttttcttttg ttcagcaggg tatgtgttca tgggtatttc ttgtctgatt
     9961 ttcggtatag cattagctgt cggttttttt attgcttctg gtatgaaaat cacacgttta
    10021 aatcactttt tgcaccatcg gaaaatacct gaaaaagtat tagcagaagc aaaagaaaaa
    10081 gaagaagagt atcagcgaag ttttggcttc tcactgattg caggaattgg attatgtatt
    10141 ttttcacttt tcccactttt ggcttcttta atgtggtata tggatggtag tatcggtgcc
    10201 tctatatttt ttgttacggt aggaacggga gtcttcttga tcatttatgg cagtctggtc
    10261 cgtcattctt atcgtcaatt cactcaaagt gcttattact ggtgaatgta ttcagatttg
    10321 taaaaatatt ttatttattg cttacccatg taaaatcgtt atagacagat ttcagttgac
    10381 ttctgcgcca ttgtacaggg ataaaaagac gcatactatc ttcattgaat ccgactaata
    10441 atttctttcc gtcagtaatg atgggacgtt gtaacaaagt aaaatgcgag acgatcagat
    10501 cgacaagctc tgaggtcgta cattggtcga tttgatcttt caaattttta taatcaatcg
    10561 agcgtttagc taatagatcg tcaaatccgt tttctgttaa tgacaataaa taaaatatct
    10621 ctttccttgt taaaggctgt ttaggtgtga aaattcgttc tctgtaaggc atttgatggt
    10681 ctttcagcca gtttgccatc agatggtaac tatgtgtgcg agaagaatag atcgtaagca
    10741 taaaaaaact ccttaaatag tccattaatt tgttagatct gttgagataa ataataggta
    10801 aggagctgtg cataagcatt gatcaaaaca acccaaacat cattagaaat aactttctaa
    10861 agtcttaaac ttgcgacgag aaacaactag actaatagaa aaaatgtctt agccatttgg
    10921 cttcctgttt gtttttttgc ttatgctgca gctcctcggg aaagggttct aacaatttga
    10981 agaaggttga tgattgatct agtttaaatt gtgtgaaatt tgaaatctgg tgtttttact
    11041 agatgaatca cctaacaact atgattataa cttatagaac ttctgttttg tacttggttt
    11101 tgttatagtt gttataaagt tttattttat tacagaacat ttttaattaa
//
LOCUS       DAMEET010000082        10933 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.083, whole genome shotgun sequence.
ACCESSION   DAMEET010000082 DAMEET010000000
VERSION     DAMEET010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.083"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..150
                     /locus_tag="PB780_002079"
     CDS             <1..150
                     /locus_tag="PB780_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDE0734123.1"
                     /translation="LEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLR
                     YSLGY"
     gene            complement(231..1214)
                     /gene="istA"
                     /locus_tag="PB780_002080"
                     /pseudo
     CDS             complement(231..1214)
                     /gene="istA"
                     /locus_tag="PB780_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311512.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(1609..2451)
                     /locus_tag="PB780_002081"
     CDS             complement(1609..2451)
                     /locus_tag="PB780_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDE0734124.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGI
                     PYINNLGLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQH
                     LEIS"
     gene            2776..3192
                     /locus_tag="PB780_002082"
     CDS             2776..3192
                     /locus_tag="PB780_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDE0734125.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3189..3659
                     /locus_tag="PB780_002083"
     CDS             3189..3659
                     /locus_tag="PB780_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDE0734126.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3673..4446
                     /locus_tag="PB780_002084"
     CDS             3673..4446
                     /locus_tag="PB780_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDE0734127.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4460..5272
                     /locus_tag="PB780_002085"
     CDS             4460..5272
                     /locus_tag="PB780_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDE0734128.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5326..6291
                     /locus_tag="PB780_002086"
     CDS             5326..6291
                     /locus_tag="PB780_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDE0734129.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6428..9151
                     /locus_tag="PB780_002087"
     CDS             6428..9151
                     /locus_tag="PB780_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDE0734130.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            9164..9796
                     /locus_tag="PB780_002088"
     CDS             9164..9796
                     /locus_tag="PB780_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDE0734131.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            10001..10228
                     /locus_tag="PB780_002089"
     CDS             10001..10228
                     /locus_tag="PB780_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HDE0734132.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10297..10563
                     /locus_tag="PB780_002090"
     CDS             10297..10563
                     /locus_tag="PB780_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDE0734133.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10553..10822
                     /locus_tag="PB780_002091"
     CDS             10553..10822
                     /locus_tag="PB780_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDE0734134.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         3627 a         1740 c         2136 g         3430 t
ORIGIN      
        1 ttagaaaaat taggttgtca aaatttcaac tcacgttctg ttaaagggct agagagtaga
       61 tttgaaagca tattactggc ttacagtgtt ctattaagtc gagcacaacg acgttttgaa
      121 ggaactttga gatattcttt aggatattaa aattaactag caccacgggt aaaataaata
      181 gtttttctat caagtatatt gaactacgat aaatttgtta ttttgttttg tttaatttcg
      241 ttgtacttat tggctttgac ttcgatttct ttaaatactg ggtaatcggt gtccggcaaa
      301 gctagcaaat ctgttttttc tgtgttccat agatcattga taatgacatg tttttcgtaa
      361 tggattcgct ctcgatcgtg tgacagctcc accgctaatg cctcgttgaa actggcgaaa
      421 tccttcataa tcggtgtaga tgaaaagaag ttgtaccgta catagccgac tttgttttcc
      481 atactacctt tctcgtttcc gctacgtgga ttgcacacct gtgtctcaaa accatagtgc
      541 atagaaaatc ggaggaaacc gtcagtcagc accgcttgtt cagtctttgt ttttgtctaa
      601 gtaacggccg ttgacatatt atcaatccgt attttccgag gaacgccacc tgattggctg
      661 aagagttctt tcatgccttc caacaaacat tcttgattct cggcggggag tacaacggcg
      721 aatcctgcat tactaaaagg gaaagtcatc actaatacct tcacatcttt aaacgctcca
      781 tcgtgacaaa cttccatcgt accaaaatca agttgcgctt ctgctggcgg gtgttcaaga
      841 cgctcaaagc cttggtcttc cagtgtttcc aagtagtgtg tcgctttcca atcttgaatg
      901 aatacacaga ctgttcggta ggatcctgga aagccatcgg cttttaaagc agtagtcatt
      961 gcttcagcag tcctacgctt tttatttggc agcttagcat cttctgaaag ccatagggaa
     1021 acgatgcctc cccacttttc tgtatacatc ataccagtct ttttgcgtgc tttaggttgc
     1081 ggcagcaaat catcatctgc atatttttta gccgtgcgcc aatttacatc tagctccttt
     1141 gcaatatgat taatggattt atcatgatta tttcgtaaat ttctgatagt attaatatcg
     1201 gtcattgtga gcataccttt cttacctcca gttacagtgt cgagactata actttagagg
     1261 agtttcaggt tgcccgcaat ggctttttat cttaacttaa tcgatttgcg gagttgtgta
     1321 gtttttcgtt gcacttgtat gtacttttat tctgccataa acagcttaat gaatgtccac
     1381 ttgaaaaagt gaataaacga aatagatgac tcgatttaat aaggaagtaa acccacacag
     1441 aaatataggt gattttcctt gaatgaccaa gatattgaaa gttagtgaaa aaacacaaaa
     1501 aggtagccct attcacagaa ggtctgcctt ttgtgttatc gccagcaaac attagagggg
     1561 gtatttcata tagcttttgt cctctcgaca gtcctatcag tttctctttt aacttatctc
     1621 taagtgctgt ccagttttaa tagcttctag cttgtttcct aactgtgttt ttaatatgtt
     1681 gaaggctttt gaacctgtgc agtggcaagt aaggattttt tctactcgtg agtcattcaa
     1741 tttatttcca attttcatta cctcttgctc aggtagacct aaattattta tataaggtat
     1801 gccaatcata tggaagccac caattaggta ttttatgggt ttagtcggaa atgtttcaag
     1861 agcaatttcc atcatatcga taatgccatg atgactacag ccagtaaaga caatcagacc
     1921 gtcttcttgc tcaattacga ccaataattc atctgaaaaa tcatcataaa caaatttatc
     1981 atccatcttt ttgtataaat tcttggtggt cgagtttgct ttgcctaggg ggatgatatg
     2041 gaagctcgga gcaagttctg ttttcgtttt tattgtatgt aaattctcat gcattttaag
     2101 gatttcattt ttaggaatac caacatacag aaacgtggtt aaaatcttta aataatgggg
     2161 taaagttaca ccttcagctg tataaatagg ggcgtttcct gattttaaat atcttaaccc
     2221 gccggtgtgg tcagaatgac cgtgtgaaat aatcactgcc tccagatcat tcaaagtttc
     2281 ccccatttta gtcgcattat agactaattt tttattagcg ccagcatcga agagatattt
     2341 cttccctgag tagttaatta agagagataa cccatgtcca cttttaaagt tcaaatttat
     2401 tcttgagttt tcaaataaaa ccttgatttc cactataaca tctcctttca catgaacgtg
     2461 tattcatata ttgtagcaca ttaagttcta ttttttgttg taattaccga aaaaaatatt
     2521 aatggacgct ttttacgctt tatttcgtat gtggaagaaa atttcttagg atgtggcggg
     2581 ggattggggc aaagctaaat tccaattaca gtgatcattt aattagacac ctttaaatac
     2641 ccacactttc atcaacacta taatttaggg aaagaaaagt gttggcatga tatagaaaaa
     2701 cctttaatta acagcgtttc ctaaaattgg cacgcatctt gcttatagaa aagcgtaagg
     2761 ataggaggaa tgaaaatggc aactataatt cttgccagcc atggggaatt tgctcaagga
     2821 ttaaagcaaa cagcgacaat gattattgga gatagtgtac caattcatgc cttgtcagca
     2881 tttagagatg aagatgaatc tattttggta caaataaaaa aacttttagc cacaatagat
     2941 atagaagaaa cctacatttt aactgatatt ctaggtggta gtgtgaataa tgatatgctc
     3001 actattatta aagagtatga gggattgcaa ttaattactg gaatgaactt acctttagtt
     3061 atttctattg caacctataa tggaaaaatt ggggaaagtg agctagaaaa gcttattaaa
     3121 gagagtcaac aatcagtgat tgattgtaga aaattattag tagaacaact tactggagga
     3181 gatgacctat gattaagtta gtacgtattg accatcgttt gttgcacgga caggtagtat
     3241 tttcttggag taaatcatta aatatcaatc gtattattat tgccaataaa gaggcagcga
     3301 atgatgaatt caaaaaaatg tctttgaatt tatctaaacc gactggcatt aaattaacaa
     3361 ttttttcagt tgaacagtta ctagaaaaaa tgcctaaaat cgaaacactt aacgaaaata
     3421 tcatgcttat ttttggtaat acaaaagaaa ttcgtcaatt ttgtgaagcc tatcctaaag
     3481 tacaagaaat caactatgga gggataatca aaaaagaagg ttccaagcaa tttagtaatg
     3541 ccattttctt gaatgaatct gagcaagaag atgccaaaaa attaaaagct cttggcatta
     3601 aacaatttat tcaacaggta ccaacttcta aaaaagaaga tttgaataca atgttataaa
     3661 ggaggaatga aaatgctcgt tcagtcaatt ggtttagggt taatcggtgt tttttgtatt
     3721 ttagactcaa ggcttttagg aagaatgaat tttgaacgac cgttgattgt cagtacgctt
     3781 gttggtttgg tacttggaga catggaaaaa ggattaattg ttggagcctc tttggaattg
     3841 atgtccttag gattagtaaa tatcggagct gcttcaccac ctgatatgaa tatggcatca
     3901 attattgcta cagcctttgc cattttatca aatgcgaatg cagaagcagc attaacgatt
     3961 gccattccaa tttcagtgtt aggtcaaata ctaggtatct taatgcggac gattttgtca
     4021 aatttgactc acgttgcgga tcgctttatc gaacaaggaa aatttagcca agcaaggaga
     4081 attcatatac tttatggctc gggattatat gctctcatgt attttattcc tatttttcta
     4141 gcaatttatt ttggaacgaa tcttgtttct cagattgttg atgcgattcc tgagtggcta
     4201 acaaatggct taacggttgc aagtaaaatt ttaccagcat atgggtttgc gttgttatta
     4261 caaacgatgt taacgaaaaa aatggtgcca tttttattac ttggattttt aattactgct
     4321 tattctggat tgggaatcat tggtattgca gccttcgcat gtattgttgc gtttgtcatg
     4381 gttcaagttc aaggtaaaaa ctcaaatggt aaccaaaatg agccagatcc attagatgac
     4441 ttataaggag gtagattaag tgaaagagaa agatcaaaat gcatggaaat atcggcagtt
     4501 cttatggcgt tcatgggcaa ttcaagcctc atggaactat gaacgacaaa tgaacatggg
     4561 ttttatgtac ggaattgcac caattctaga taacatttac aagaatccag aagatgaagc
     4621 aatgaagaaa gaagcttacg agcggcatat gatgtactac aactgtaccc ctcaaacaag
     4681 tgcatttgtg ttagggttaa ctgcttctat ggaggagcaa tactacgaag accaagaaaa
     4741 ctttaatccg gaatcaatca gtgcagtgaa gacttctctg atgggaccgc tatctggtgt
     4801 tggtgattct ttctttcaag gaacgattcg ggtattagcc tttggactag gaattaattt
     4861 agcccaacaa ggtagtattt taggtccaat tctagcaatg ttaatttcct ttgtaccttc
     4921 ctttttagtt acttactatg gtgggaaaat tggctataac atggggaata aatacttgtc
     4981 taaactgtat caagaaggtt tgatggataa agtaatgtat gtttgctcca ttgtggggtt
     5041 gatggtcatt ggttcgatga ttgccagcat gatcgggatt acaacaccaa ttcaaatggg
     5101 taaagctttt gtcttacaag atgtattaga tagtattatg ccacaatttt taccattagg
     5161 attaaccttc ttcatgtact ggttattgca gaaaaaggtc aaaacaggat ggatgttggc
     5221 aatctgttta gtcggaggaa ttatttttag tttgttaggt atcttacaat aagggaataa
     5281 ccaataaatt tggttgaata atataaataa ggaggaattc aattgatgaa ttcaaaagaa
     5341 attgttaaag aaatcgtagc gaaaaaagag gtaaaatcaa tcgtatttgt tggctgtggc
     5401 gcttcaaaag cggaccttta tcctgcaaag tattttctag accaaaatgc gaagcagtta
     5461 cggattagtc attacacagc cagcgaattt aactatgcaa caccagcatc tgtagatgag
     5521 tcaactgtcg ttatttcagc ttctttagga ggtacgactc cagaaacagt tgaagctaac
     5581 gcagtagcta aagaaaaagg tgctacagtt attagtctaa cacgaagcat tgactcaccg
     5641 ttaacaaaag atgcagatta tgtaatttat catcgatttg cagagaacta tgctgctaaa
     5701 ttagaaaaaa caggctactg tttagaattg gcagtagaac ttttacatca agtagaaggt
     5761 tatgaccatt atgaagcaat gcaagaagga tttgctacta tctatgatct tgcccaaaaa
     5821 agtgcagaat cagctcgtaa agatgcgaag aaatttgctg aacgctttaa agatgaaaaa
     5881 gttatttatg taatgagtag tggtgctaca catgaagtag catactcaac atcaatttgc
     5941 ttgatgatgg aaatgcaatg gattaattct ggtaccttcc attcaggaga atttttccac
     6001 ggaccattcg aaattgtgga taaagacgta ccgtttatat tattaatgaa tgatggaaaa
     6061 actagaaata tagatgctcg agcattaact ttccttgaac gctttgatac gaaaacagaa
     6121 gttgtagatg ccttggatta tggcttgtct tcacatattg ctaaagaagt cttagactac
     6181 tttaacccat tcgtgattac tgcagtattt agagtttatg cagaagaatt gtcagaagca
     6241 agagaacatc cattaactaa gagacgttat atgtggaaac taacttatta aaaaatagat
     6301 caaactaaat aaggtggctt gagaaattaa gccaccttgt tttcttcaat gtgattttat
     6361 acatatggta aaataactag tacatatggt aaaataacta gtacatattg tgaaagcagg
     6421 tggcaagatg cagcgattaa atgactatct ttatcaacta acattgcttt tgaaatctac
     6481 gccttcagat aattattcag ctcgcagtat ttcgcaagct ctacagttga atagaagtac
     6541 aatcagtagc tacttaaatg aaggtgttcg tgaagggctt ttcataaaag taaaaagtta
     6601 cccggtttta tttttacata gaacagctct tgaagaacta catatcacac tgaataactc
     6661 cgaaatagaa tcaattgaaa gcttgttaac aatttctcaa aaacccgcat tagatcaagt
     6721 aattggctct aaaggaagtt tgaaagaagc aattgatcaa attaaaacgg cagtcttata
     6781 tcctggaaaa gggttgcctt tattattgat cggcgctagt ggatcaggga aaacctttct
     6841 agcgaataaa atatatgaat atgcagtaga agaaaaggtc attcacacat cggcaccttt
     6901 catagattat aattgtgcac aatatgtgag caatccagag ttactatcct ctgctttatt
     6961 tggctacacg aaaggagcat ttaccggagc aagccaggag catactggtt tgctagaaaa
     7021 agccgatgga ggagtattgt ttttagatga agtgcatcgc ttatctgaag aagggcaaga
     7081 gaaattattt acatttatgg atacgggtga attctctcct atgggggata atagtattcg
     7141 gaaaaaagcc gatgttcgat tagtctttgc aacaacagaa aatatatata cgactttctt
     7201 accaactttt cttaggagat tgccagttat tgttaatcta cctagatttc aacaacgtcc
     7261 gtcgtttgaa cgtctgtcat tgattgatga attttttgtt tctgagagcc aaattttagc
     7321 taaggaacta tctgtttctg atgcattgat tcatttctta atgaatagtg attttgaagg
     7381 taacgtgggg aatattaaaa atattatcaa gtatgcttgc ggaaacgcat atatccacca
     7441 gaagagtcag caaacaattc aagttaaatt attggatatg cctttagagt acagttataa
     7501 attcaaggag caactaaata aacccagaaa gaaaagagca gatcgaatct actatccaga
     7561 agaaacacga caagtgcaac tagaaagaaa caatcagctt tttgttcaat tttttgacca
     7621 attacttact ggattctctg aagtgattga aaataacaag gatattaaac tatttttaga
     7681 agaaatggtt accaaggtaa cacaagtaat ggatacgctt atttttcaag aggattacga
     7741 aaaagagcag tctttatacg ccattctaaa ctatcatatt cgtcaaagta ttgattttat
     7801 gcgagaaagc tacgggtttg aacaagatgg taaccgggta gtcgctttgg ccaactatct
     7861 atatgtcaag gataatcaag tgatttttga aaatagtctt caagaaaaga aaaccaaaga
     7921 tcagcttaac gaattttttg ctaatgttct tgaaacccct tattggtatg caaaaaaaat
     7981 cttaagcctt ttatctgagt gtttagatca acctgtgtat gaagaggaca ttttctttat
     8041 tacgctatat ttttatagtt tgaatgtcac gaatgttaaa acaggtatta agagtattgt
     8101 gctagctcat ggttattcta cagctagtag tatggcgaat gttgccaacc gtatgctgaa
     8161 taagaacgtt tttcaagctt atgatatgcc tttagatatt acattagaga aaattgaagc
     8221 aaaattgatt caattccttg gagattatcg aacagattca ggcttgattt tattggtaga
     8281 tatgggttca ctaaaccaac taggcggttt attagtgaat catctaaatg gaccactgtt
     8341 aatcattgat catatcaata caccgttatt attggaagtc ggccaacaag tattaaacaa
     8401 taaaagtatc accgaaatag gagcagaaat tgaaacaagt cttactttca aaaagcagtt
     8461 actacttccc aagaaaaaga aaaagaaagc catcattact tgttgttata cagggattgg
     8521 tagtgcgacc cagattcagg agattttaac gaaatgcttg aaagatgcag ttagagaatt
     8581 agtaattatt ccgtatgatt acaaaaagct gttagaaaac aagaattttg agactccttt
     8641 tcaattatac gatgtcatca tgattgtagg tacagaagat cccaaaatta atcagattcc
     8701 atatattgga ttagatcgcc tgattaatgg gggaagcatt ggcgaatttg ttcattactt
     8761 ggaaaaagag ctgactattg aagtgaacga tttacaaaaa gaattaattt tcaatttttc
     8821 aattagtaaa attgtcgaaa atttaaccat tttggatcca gataaggtgc tatcttttgt
     8881 acaaaaagca attaaagaat tatcgaagtt attaaatttt gatttttcta ataatcagct
     8941 gtttttgttg taccttcact gtagttgtat gattgaacga attgtacgta aagaaagtgt
     9001 ggatgagcaa gaggacattg aagaatatat gcaagcaaat ggagaaataa tggataaaat
     9061 tcatagcgct ttttttgaga ttgaaaggga atacacaatt gaaataccag atttagagtt
     9121 gcgattattg agcgatattg ttcaaggata ggagtgaatt cgaatgacaa tgggagtaat
     9181 ttttgatatg gatggggtac tcatagatag cgagacattt tattttgaga gaagaatgaa
     9241 cttctttcaa gaactggaga tagatccagg atcaaaccag attgatgatt atgtggggaa
     9301 aacagaagct gcaatatggg aaacattagt tcctgaggac aaatcattaa gagaggaact
     9361 ttatccgaaa tatgtagatt atcgaaaaac gcatccaatt gatttcgcta aagctttacg
     9421 aaaagaagtg aaaccgttac ttgacttcct caagaagcgg gatataccat tggcactggc
     9481 atcttcttct ccagctgtcg agatagagag tatgctcatt caatgtgaac ttactgaata
     9541 tttttccttt gttataagtg gtgagtcatt aaaacaaagc aaaccacatc ccgaaatata
     9601 tttaaaagcg aaacagcaat tagactgtga cttctttatt gcagtagagg attcaacgat
     9661 tggtattaaa tcagcaaagg cagcaaatat ctatacggcg gcactaaaac aaccaatgca
     9721 aatcgatcag agtgaagcag acatacaaat tgaaagtcta caagaactgg aacaaattgt
     9781 tttagaaaaa ggttagtgaa ttaatggaaa taaggctata tatctgtgct agctattttg
     9841 actaaccaaa aaaacttgct ataattcagg tgtagtaaaa ggtgctggta acacctttta
     9901 cgaaaatgct tcggcagtga taatgggatt ggctttttaa gttttttatt ttgttcgcaa
     9961 tccctaaggt catgtgaatg atcctaggga ttgctagaat attttgcaac agaaccaaaa
    10021 tacgaatagt tatcaagcag ccatattgac tgcggtcaat ttaggagggg atactgatac
    10081 tactgctgcc gtaactggtg ctcttgcggg tattctttac gggggtgaag tgatccctga
    10141 aaattggaga aaatcgcttc aaaatgacca gcttcttaaa acgatcagtc agagattttg
    10201 cgataccgtg ctatctcaaa gaatttgaat aatggatatg atgcaaatct tgtttgtact
    10261 aacattgtat tgacaaaaag gattttattt agtacaatgt tagtacaaat gatgaacgga
    10321 ggtagaatca tgtctcaatc aaccattaca tttaggattc ccgacgatga aaaagaactg
    10381 gtattagaat atgcaaaagc acacaattct tcattgaccg aactttatcg aaatgctgta
    10441 ctggataaaa tcgaagatga aatcgatcta cggactttac ggagtgcaat tgaagtttcc
    10501 aaagagaaaa aagagagtgg tatttctcaa gaggaaatgg agaaactgct aaatgaatta
    10561 taaagtcatc tacatgccgc aagcccagaa gcaattaaaa aaaatggaca gaaaccaagc
    10621 taaaataatt gtcgcctgga tcagaaagaa tttagaaaac acgactgacc cgcgtcagca
    10681 cggaaaaggt ttgactggaa ataagagtgg ggaatggcgc tatcgaattg gaaactatcg
    10741 aatattggct aatatttgtg attctgaaat tgaaatccag atattctcaa ttggacatag
    10801 aagtacaata tataaaagat aaccgttttt ggaacgaagt agagtttcga aagtcaatgt
    10861 ataatctttt gaattttgca taataccaca ggtagaaact acccgatact atcggttggt
    10921 ttctgctttt tta
//
LOCUS       DAMEET010000083        10711 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.084, whole genome shotgun sequence.
ACCESSION   DAMEET010000083 DAMEET010000000
VERSION     DAMEET010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.084"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1..957)
                     /locus_tag="PB780_002092"
                     /pseudo
     CDS             complement(1..957)
                     /locus_tag="PB780_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
     gene            1448..2386
                     /locus_tag="PB780_002093"
     CDS             1448..2386
                     /locus_tag="PB780_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDE0734135.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAV
                     KKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRD
                     MVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIRE
                     ISPIDLTELMKSTLKANEKQLQKQIDFWQREFRFWK"
     gene            2516..3163
                     /locus_tag="PB780_002094"
     CDS             2516..3163
                     /locus_tag="PB780_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-7 chloramphenicol O-acetyltransferase"
                     /protein_id="HDE0734136.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNS
                     SFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
     gene            complement(3856..4794)
                     /locus_tag="PB780_002095"
                     /pseudo
     CDS             complement(3856..4794)
                     /locus_tag="PB780_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(4794..5411)
                     /locus_tag="PB780_002096"
     CDS             complement(4794..5411)
                     /locus_tag="PB780_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDE0734137.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            complement(5425..5595)
                     /locus_tag="PB780_002097"
     CDS             complement(5425..5595)
                     /locus_tag="PB780_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734138.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            complement(5943..7433)
                     /locus_tag="PB780_002098"
     CDS             complement(5943..7433)
                     /locus_tag="PB780_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HDE0734139.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            complement(7476..7775)
                     /locus_tag="PB780_002099"
     CDS             complement(7476..7775)
                     /locus_tag="PB780_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734140.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            complement(8141..8425)
                     /locus_tag="PB780_002100"
     CDS             complement(8141..8425)
                     /locus_tag="PB780_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDE0734141.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            complement(8944..9429)
                     /gene="vanZ"
                     /locus_tag="PB780_002101"
     CDS             complement(8944..9429)
                     /gene="vanZ"
                     /locus_tag="PB780_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HDE0734142.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(9582..10493)
                     /locus_tag="PB780_002102"
     CDS             complement(9582..10493)
                     /locus_tag="PB780_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HDE0734143.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         3308 a         1864 c         1658 g         3881 t
ORIGIN      
        1 acttttttct gcttcttttt ctttctgttc ctggtaatca gaaaaattta gtttttctat
       61 atcgttaggt acagcttcca gtaaatgaac aatgaatttt ttgatattcg taataaagtt
      121 ctcttgattg ccttctcttt taccgatttt ttttaaatac gtttcccatt tagccgtcat
      181 ttcagcactc gttaaaaggt gctgactttc aactgcctgg cacaataatt ttcctttttc
      241 agttacaaca agcttattct taatcacttg gatatattct ttttgtttta acgcttcaat
      301 aatacttgct cttgtcgctt ctgttccaat cccctcaact tcttgtaaaa tcttgattgc
      361 ttcttcatca tcaaccgttt tattcgccgt tttcatagca gttaataatg taccctctgt
      421 aaaagctttt ggcggttgcg tttctttttc aacactctta acaccaactt cggcacgttc
      481 gccaatgata acgagtggta acgtttgaac gtcctctttt tcttctttgg tttgttgttt
      541 gaaaagaatt ttccaccctt cttgctttgg tgtctttccg atcgattgaa acagcaaatc
      601 agctactttc gtttgtatct tggtttcttc atacaagtaa tcaggtaaaa acatggcaac
      661 ggtcgtttta acgactaaag cataaatttt tcgttgtaaa tcgtccattt tcgctaatgc
      721 agattcggta ggtacttgtt ttgttgggat aatggcgtgg tgttcctgta ccttactacc
      781 gtccacataa cgctttctag gctctgtttg aaccatttca agatcaagtc ctaaaaaacc
      841 gctatatttg ccaaaattcg cttttaaata agcaaattcg ttctctgtaa taaatggtgt
      901 atctgttctt ggataactca ataattttgc ttcatacagt ccttgcatag cttttaaacg
      961 taaaattttg cgttctaaga cgttttaaat atatctgcgt atatttttat gtgttttatc
     1021 ttttcgtcgg caaatgcgtt aaaatttgcg aaataaaata gctcaagggc gaacgcagtg
     1081 agtgcattta cccttgatct atttttttga gtaaattagc ttttgaaacc gacggaaagg
     1141 aaacacaaaa cttttcagag gcaaggggtt tttaaggggc gcttttagcc ccttgaattt
     1201 atttgcttta agatatattt agttttaaaa tagttttaac gaaaaaaaga cataaaaaaa
     1261 gacgagcaac gctaccaacg ttctcgcccg gaataacaaa ccacacttgt tattcattcg
     1321 atgattagta tatcaatttt tgattttgaa tacaaaacaa aatggacgaa tggatacagg
     1381 cgaaatttat tttgcgattc cactcgtcct tttttcgttc caaaaattat tttaggagcg
     1441 ataaaaaatg aagaaaattg atgaaaaatt agctgcagaa atctcactaa aaaaaccggc
     1501 cgtctctaat agccggttaa gtaatagccg gttaagtggt caaactttgg gaaaatctca
     1561 acccgcatta agttttgatg ccatgacaat cgttggaaat ttgaacaaaa ctaatgctaa
     1621 aaagctatct gactttatga gtgtagagcc acaaatacga ctttgggata tacttcaaac
     1681 aaagtttaaa gctaaggcac ttcaagaaaa agtttatatc gaatatgaca aagtaaaagc
     1741 agatacttgg gatagacgta atatgcgtgt tgaatttaat cccaataaac tcacacatga
     1801 agaaatgatt tggttaaaac aaaatattat cgactacatg gaagatgacg gttttacaag
     1861 attagactta gcttttgatt ttgaagatga tttgagcgat tactatgcaa tgactgataa
     1921 agcagttaag aaaactgttt tttatggtcg taatggcaag ccagaaacaa aatattttgg
     1981 tgtccgtgat agtgatagat ttattagaat ttataataaa aaacaagaac gtaaagataa
     2041 cgcagatgtt gaagttgtgt ctgaacattt atggcgtgta gaagttgaat taaaaagaga
     2101 tatggttgat tactggaatg attgttttaa tgatttacac atcttgaaac ctgcgtgggc
     2161 tactttagaa aaaattaatg agcaagctat ggtttatact ttgttgcatg aagaaagtat
     2221 gtggggaaag ctaagtaaga atactaagac taaatttaaa aaattgatta gagaaatatc
     2281 tccaattgat ttaacggaat taatgaaatc gactttaaaa gcgaacgaaa aacaattgca
     2341 aaagcagatt gatttttggc aacgtgaatt taggttttgg aagtaaaata agttttattt
     2401 gataaaaatt gctaattcag tataattaat atttacgagg tgacataacg tatgaaaaaa
     2461 tcagaggatt attcctccta aatataaaaa tttaaaattt aggaggaagt tatatatgac
     2521 ttttaatatt attgaattag aaaattggga tagaaaagaa tattttgaac actattttaa
     2581 tcagcaaact acttatagca ttactaaaga aattgatatt actttgttta aagatatgat
     2641 aaaaaagaaa ggatatgaaa tttatccctc tttaatttat gcaattatgg aagttgtaaa
     2701 taaaaataaa gtgtttagaa caggaattaa tagtgagaat aaattaggtt attgggataa
     2761 gttaaatcct ttgtatacag tttttaataa gcaaactgaa aaatttacta acatttggac
     2821 tgaatctgat aaaaacttca tttcttttta taataattat aaaaatgact tgcttgaata
     2881 taaagataaa gaagaaatgt ttcctaaaaa accgatacct gaaaacacca taccgatttc
     2941 aatgattcct tggattgatt ttagttcatt taatttaaat attggtaaca atagcagctt
     3001 tttattgcct attattacga taggtaaatt ttatagtgag aataataaaa tttatatacc
     3061 agttgctctg caacttcatc attctgtatg tgatggttac catgcttcac tatttatgaa
     3121 tgaatttcaa gatataattc atagggtaga tgattggatt tagtttttag attttgaaag
     3181 tgaatttaat tttatacacg taagtgatca taaaatttat gaacgtataa caaccacatt
     3241 ttttggttgc ttgtggtttt gattttgaat ttggttttga acttatggac tgatttattc
     3301 agtccatttt ttgtgcttgc acaaaaacta gcctcgcaga gcacacgcat taatgactta
     3361 tgaaacgtag taaataagtc tagtgtgtta tactttactt ggaagatgca ccgaataaaa
     3421 aatattgaag aacaactagc aaaagatttt aaagagttat tttattttaa gtctttataa
     3481 catgagtgaa gcgaattttt aaatttcgat agaaattttt acatcaaaaa gccccctgtc
     3541 aaaattgacg aagggggttt tttggcgcac gcttttcgtt agaaatatac aagattgaaa
     3601 atcgtgtata agtgcgccct ttgttttgaa cttagcacgt tacatcaatt ttttaaaatg
     3661 atgtataagt gcgccctttt aaattttgag tgattatatt ttttgagtta gaaaaaggga
     3721 ttgggaaaat ttcccaaaat aatttaaaaa ataagcaaaa attttcgata gagaatgtgc
     3781 tattttttgt caaaggtgta taccttgact gtgcttgctg ttacattaag tttattttta
     3841 agttattaaa aaagaaatag cttttaaagt ttggctcgct gtcgctttat aaagctgatt
     3901 gacttttgat tgcaaactac ttaaagaaaa caaactcgga ctattcgttt tcttctcttt
     3961 ggtttgaaca tcagcaatta tcccctcttg attgcctatt ttagcttgtt tagaagaaac
     4021 aaaagctaaa agctcctctt gggttttaaa acgctgtgtg gggcttagaa cgcccttaaa
     4081 cgacccttgg tttactttta tactagcttc cacctcgaaa aaaggttctt ttttaaaatt
     4141 ctctatggct tcctggcgct gaaaaataag gtataaggtg ggcgtttgaa cacgtcctag
     4201 tgaaaatgta ccttgtacgc ccttctgttg taaatttaac gtatacaaag ggcttgcgtt
     4261 catgccgatc aaccaatcgg caatttggcg tgtttgcgct tcttgataaa agggatagta
     4321 attcattcca ggttgcaaat tttgaaaacc gcttcggatt acatcttttt ctaagctatt
     4381 gatccatagt cttttaaatg ttttatcttt tgaaaaggca tttgctttat ggataatcga
     4441 ccaggcgata ttttcacctt ctctgtcgct atctgttgca acaataattg tatttgcctt
     4501 tttgagaagt tctgcaacaa ttttaaactg ctttccctta tcttttgcaa cttcaaaatc
     4561 gtatcgatca ggaaaaatcg gcaaagattc aagtttccaa ttttgccact tttcgtcata
     4621 atgacctggt tctgctaatt ccactaaatg cccaaaacca aaggtgataa acgtttcatc
     4681 tgtaaatagt gggtctttga tctcaaaata accgtctttt ttggtgcttt gttttaaagc
     4741 acttgcgtag gctaatgcct ggcttggttt ttcagctaaa ataaccgtac tcattaacta
     4801 tccctctttt cattgttttt tctttgatcg actgtcacgt tatatcttgc tcgatacctt
     4861 ctaaacgttc ggcgattgat tccagtttgt tcttcaactt ctttatcgga taaaccattc
     4921 aaaaacaaat cgaaagcatg ctttaaacgt ggatcatttt ctttaaattt atgctgacga
     4981 cctttgaatt tgcctttttt cttagcaatt tcgattcctt gtgcctgacg ttccttaatt
     5041 ttttttcgtt ctgattctgc ttgatacttg tacaattcaa tgacaaggct attaatcaaa
     5101 cgccttaaat tttcatcttc aataccattc attgagggta aatttaagac ttccagggtt
     5161 gcccccttaa tttgaatttg attcatcaat tctgttaatt ctttattatt tcgtcctaat
     5221 cgatctaatt cagtaacaat aacaatatcc ccttcacgaa tatagttaag catagcttgt
     5281 aattgtgggc gttcgaccga ttgaccgctt aatttgtctg aaaagacctt agaaacgccc
     5341 tgtaacgctt gtaattgccg atctaagttc tgttctttgc tactgacacg tgcataacca
     5401 attttagcca ttttcaacca acctctaaaa ttctctcggt tgcaataacc aatcagcaat
     5461 atctactttt tcaatttcaa attgcttatc agaaattgtc ttttcgtaag cgataaaatc
     5521 ttgcgcatat tgttgctcat taaaaatagc caccacttcg tcattttcta aaactcgata
     5581 aataaatttt ttcattttac tcctcctatt atgcccaact taaatgacct attcaccaag
     5641 tcaattatac tgctaaaatc atattaggac aaataggtat actctaatga cctataaaag
     5701 atagaaaatt aaaaaatcaa gtgttcgctt cgctctcact gcccctcgac gttttagtag
     5761 cctttccctc acttcgttca gtccaagcca actaaaagtt ttcgggctac tctctccttc
     5821 tccccctaat aattaattaa aatcttactc tgtatatttc tgctaatcat tcgctaaaca
     5881 gcaaagaaaa aacaaacacg tatcatagat ataaatgtaa tggcatagtg cgggttttat
     5941 tttcagcctg tatcatagct aaacaaatcg agttgtgtgt ccgttttagg gcgttctgct
     6001 agcttgttta aagtctcttg aatgaatgta tgctctaagt caaaagaatt tgtcagcgcc
     6061 tttatatagc tttctttttc ttcttttttt actttaatga tcgatagcaa caatgattta
     6121 acactagcaa gttgaatgcc accatttctt cctggtttaa tcttaaagaa aatttcctga
     6181 ttcgccttca gtaccttcag caatttatct aatgtccgtt caggaatgcc tagcacttct
     6241 ctaatctctt ttttggtcgt cactaaataa ggcttgtata catcgctttt ttcgctaata
     6301 taagccatta aatcttcttt ccattctgac aaatgaacac gttgacgttc gcttcttttt
     6361 ttcttgaatt taaaccaccc ttgacggaca aataaatctt tactggttaa atcacttgat
     6421 acccaagctt tgcaaagaat ggtaatgtat tccctattag ccccttgata gttttctgaa
     6481 taggcacttc taacaatttt gattacttct ttttcttcta agggttgatc taatcgatta
     6541 ttaaactcaa acatattata ttcgcacgtt tcgattgaat agcctgaact aaagtaggct
     6601 aaagagaggg taaacatgac gttattacgc cctattaaac ccttttctcc tgaaaatttc
     6661 gtttcgtgca ataagagatt aaaccagggt tcatctactt gttttttgcc ttctgtaccg
     6721 cttaaaaccg ttagacttga acgagtaaag cccttattat ctgtttgttt gaaagaccaa
     6781 tcttgccatt ctttgaaaga ataacggtaa ttaggatcaa aaaattctac attgtccgtt
     6841 cttggtatgc gagcaatacc aaaatgatta cacgttagat caactggcaa agactttcca
     6901 aaatattctc ggatattttg cgaaattatt ttggctgctt tgacagattt aaattctgat
     6961 tttgaagtca catagactgg cgtttctaaa acaaaatatg cttgataacc tttatcagat
     7021 ttgataatca tagtaggcat aaaacctaaa tcaatagcgg ttgttaaaat atcgcttgct
     7081 gaaatagttt cttttgccgt gtgaatatca aaatcaataa agaaggtatt gatttgtctt
     7141 aaattgtttt cagaatgtcc tttcgtgtat gaacggtttt cgtctgcata cgttccataa
     7201 cgataaacgt tgggtgtcca atgtgtaaat gtatcttgat tttcttgaat cgcttcctcg
     7261 gaagtcagaa caacaccacg accgccaatc atgcttgatt ttgagcgata cgcaaaaata
     7321 gcccctttgc ttttacctgg cttggtagtg attgagcgaa ttttactatt tttaaatttg
     7381 tactttaaca agccgtcatg aagcacagtt tctacaacaa aagggatatt cattcagctg
     7441 ttctcctttc ctataaatcc tataaaatag gttgtttaat taacttggtt tgctttttca
     7501 ttcaactgtt tcaatattgc atgttttgaa aaagattttt ttcctttata agtcaatttt
     7561 tttccactaa tcgaataaat tattttgtta ttttctatta acttatatat ataatcttcc
     7621 ccctccgaag aaaaatactt atctgatttt gtttctaagt agatatttct cttttctaac
     7681 tctttcttaa acgtttctag tgtatagata tttgctaatt ttcttatctc caataaacta
     7741 ttttttatat aagttttaca ttcatcatga ttcatacaaa ctccaccttc tataaatgaa
     7801 tacaaaaaaa gcaatcaaac gatttccgat tgattgctta acaattctta aattcagtag
     7861 cttagatact tgaaaactct ctgatttccc tatataatga tagtacggtt atataccgtc
     7921 ttcaaacaaa gttaattaaa taacttctta cgagggaaga gttcatctga ctaactgata
     7981 agcgttggtt tggcaatctt atcgggctat gcatttataa aatgtcgtca aacattttat
     8041 aaatgtgtca tggctctttt ttcgtttcta ttcagttcgt tgtttcgtta tatctagtat
     8101 accgctttta aaaaaaataa gcaacgattt cgtgcattat tcacacgaag tcattgcttt
     8161 tttcttcttc catttctaaa tccaatgtta cttgttctga ttctgtttct ggttctggtt
     8221 ctgttggctc atttgggatt aaatccacta ctagcgttga gttagttaac tttgcaattt
     8281 gttctagtgt ttttatggtt ggatctgatt ttcctgattc tattcgtgaa taatttgatc
     8341 tactcatttc taattcttgg gagaattttt cttttgattt ggtacctctc atctttttta
     8401 agctttctct aaatgctagt tccaagtttc tcccccctct ttttgtgtaa taaaattcac
     8461 gctttattta aattatagct tttaatatct taataaacaa gtgattaatc acttgtgatt
     8521 tgtgattatt ccaaaagtag gtaatttcac caaccttttt caaaagtttt tatcgacgat
     8581 tattggactg gatcgtgttt cctaaagcga ttctaagcgc atgtttaatt ttaaagtggg
     8641 taattctctt taaaacgtcc tacaaagatt caaaaaaagc atggctgcta gtgggggtac
     8701 cgtcaggaaa atgcggattt acaacgttaa ggaaatttat taaagagacc aattcctcag
     8761 cactaaaatt gataattggg ctttctttgt tactatagtg ttaggtaagc tttttacttt
     8821 atcaccggtt ttcccctcac ttcacaccta cgggcgagtt tccccacatc ccgttttcca
     8881 tctaatccaa ttcattcaat atctttatgt gtatgaaaga tcagattgct tgatttagac
     8941 atcttacacg taatttattc ttaaatgggt acggtaaacg agcaataata cgagcaaaag
     9001 tatacctaca aaaataataa ctctgtctaa ttttttttga ttcatatgct tattgcttaa
     9061 accatataat ttcagtccaa gaaagcctcc aacagtattt gtaattacat ctgttatgtc
     9121 tgtcgctcca atagcgaaga taaattgaat tatttcaaaa gtaagactta aaaccagtac
     9181 aaaagcaaac ttaggtaaaa atccgatttc tttaaaattg acattcaaaa gcaagccaaa
     9241 tggaataaag attataacat tatctatcat ctctctgaaa ttcccagtag cagtaaatgg
     9301 agtcaagtta agacttcttt gatgataatt aaatactgat aaaatattgt attgtaattt
     9361 gaataacact aaccagatta gtgtcactaa atataaagct agcaatcctc tagataatat
     9421 ttttcccaat ataatttcct ctaattcttt tgtttaagtt gtatcattag tatagcaatg
     9481 tcctagttaa cgtgggtttt aaattaaaaa gctaacgtta ccccaaaagt gctagtacaa
     9541 taatgctgtt cattaatttc gttggtttca ttccgccatc cttacctcct tgaattagta
     9601 tgtgttgatc cgggaaacac tgtcacaatt acaccgtcta tattgtttcc tgatatctca
     9661 taacgaagat tagtcggcac atgaatggtg gtatttttag taacaggata ataaaagatc
     9721 tcatattttt ccccatttac actaacagaa atggtttttt cttcttttag gtaatccata
     9781 tattcctcga gaacgaaatt cttttctttc ataatcgcac tatgtggtaa accaacatag
     9841 cgaatatgcc atggttcata ttgaattcct gttaactctg ttttgtcctc tggataacgt
     9901 aaaatgaacc cgtatttcca agcattttct tctatccact ttccttcagg ggctcgttcc
     9961 attttcgtca agcttgatcc tacatctagt gataaacctg aattatgctc actataacct
    10021 gctggtaagg catactcagc ccccatttct tggtaaagca cactttgctc atcaaagtct
    10081 cgatagccac tattaataat aaaatgacta acgccaccct ttacagcatc attgaccatc
    10141 tctgaaaatt tttgtgctat ttcttttgac atataaatat tactatcaag caacccgtat
    10201 ccatttatta attcgtcatg tttagataaa ttcacgatat ctgacttcac actttcttgg
    10261 cgaacaggat atttactatt gattaatagc agatttcctt gataaacctg ttcttctgta
    10321 attgttttct cttgggtatt ttcagaagtc ccactatttt ctaaatgttc tttgggattt
    10381 tgatcataat tttgaaattc gactttttcc tgagaaaaca gtgcttcatt aacgtagtca
    10441 taacctaagt atattaagaa taacaataac aataaaaaaa acaacttctt cattttcagt
    10501 ctcctcctta actatatagt tggaagtatg aaatatttct agaatcttcc ccttaaacgg
    10561 cacaatctcg tttgagattg tgccgttttg ccccgtaaat tacagtcaat tatttcgccc
    10621 tactggattt actattatca ccaatgtaga tgaattttca taagtgatac ccatgctgaa
    10681 tttaacgagg ctgaactgcc tgttgacaat g
//
LOCUS       DAMEET010000084        10497 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.085, whole genome shotgun sequence.
ACCESSION   DAMEET010000084 DAMEET010000000
VERSION     DAMEET010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.085"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..361)
                     /locus_tag="PB780_002103"
     CDS             complement(<1..361)
                     /locus_tag="PB780_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HDE0734144.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLGI"
     gene            complement(713..1072)
                     /gene="rplT"
                     /locus_tag="PB780_002104"
     CDS             complement(713..1072)
                     /gene="rplT"
                     /locus_tag="PB780_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDE0734145.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(1125..1325)
                     /gene="rpmI"
                     /locus_tag="PB780_002105"
     CDS             complement(1125..1325)
                     /gene="rpmI"
                     /locus_tag="PB780_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDE0734146.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(1397..1921)
                     /gene="infC"
                     /locus_tag="PB780_002106"
     CDS             complement(1397..1921)
                     /gene="infC"
                     /locus_tag="PB780_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDE0734147.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1965..2090)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            2241..2873
                     /locus_tag="PB780_002107"
     CDS             2241..2873
                     /locus_tag="PB780_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDE0734148.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2875..3780)
                     /locus_tag="PB780_002108"
     CDS             complement(2875..3780)
                     /locus_tag="PB780_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDE0734149.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(3865..4524)
                     /locus_tag="PB780_002109"
     CDS             complement(3865..4524)
                     /locus_tag="PB780_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDE0734150.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     CVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVS
                     I"
     gene            4840..5784
                     /gene="mvk"
                     /locus_tag="PB780_002110"
     CDS             4840..5784
                     /gene="mvk"
                     /locus_tag="PB780_002110"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HDE0734151.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5777..6754
                     /gene="mvaD"
                     /locus_tag="PB780_002111"
     CDS             5777..6754
                     /gene="mvaD"
                     /locus_tag="PB780_002111"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HDE0734152.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6767..7852
                     /locus_tag="PB780_002112"
     CDS             6767..7852
                     /locus_tag="PB780_002112"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HDE0734153.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7849..8913
                     /locus_tag="PB780_002113"
     CDS             7849..8913
                     /locus_tag="PB780_002113"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDE0734154.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(9162..9965)
                     /locus_tag="PB780_002114"
     CDS             complement(9162..9965)
                     /locus_tag="PB780_002114"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HDE0734155.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9985..10290)
                     /gene="kduI"
                     /locus_tag="PB780_002115"
                     /pseudo
     CDS             complement(9985..10290)
                     /gene="kduI"
                     /locus_tag="PB780_002115"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            complement(10272..>10497)
                     /locus_tag="PB780_002116"
     CDS             complement(10272..>10497)
                     /locus_tag="PB780_002116"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HDE0734156.1"
                     /translation="LWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLI
                     VIIIGGILTGIFTAVEASAIAVIYKWVIRF"
BASE COUNT         3107 a         2220 c         2008 g         3162 t
ORIGIN      
        1 aaatacctaa tgacatcaag atcggcaaca caataactgc aatagaaatc actggtccga
       61 ttcctgtcat cgacatgaat ataacagcgg ttacaataca tagtaagctc atcgtgattc
      121 ttggcttgtc ccctccaagt tcaactacct ttcgaatcag ggtagctgcg attccgctat
      181 ctactagaac acgcccgaaa aaggcaccaa agaagacatt taccaagatc gatttggcgt
      241 actcagctgg ccccgtcgta tagatatgcg tcaacacatc aattactcct tgcccttcca
      301 tcgccgagtt tggtgaaaac gtgtttccga ctagcgctaa tcccatccat acagtagcca
      361 tgatgacaaa agccgaccat caagttgtag ccttttacac agtaccagac catgaaaaag
      421 aatgagataa ctaacaaaat accaataata atatcactca tgttattcct ccgtccattt
      481 tgtttttctg ataagcaaac taatatatca gtcaaactca ttctaactta tgtagttagc
      541 gctttcatta aaggatgacc ataaatgttc agttttcaat taaaaaacct taattatgtt
      601 attttagact tatacgttat tgtcctgttt catttgatgg aggattgtaa ataaaaaaag
      661 aggaaagaac ataacttttc gttatcttct ttcctcttta taattgttta gcttatttag
      721 ccaaagcttc ttttgcttgg tcagccaatg cagtaaatgc tgctgcatcg ttaacagcca
      781 agtcagctaa cattttacgg ttgatgtcga tttcagcaac tttcaaaccg tgcattaatt
      841 ttgagtagct caagccattc atacgagctg ctgcgttgat acgagcaatc cacaatttac
      901 ggaagtcgcg tttcttttga cgacgatcgc gatatgcgta gttgtatgaa ttcattactt
      961 gttcttttgc tgttttaaat aaagtgtgtt ttgaaccgta gtaacctttt gctaatttta
     1021 gcactctttt acgacgttta cgagttactg ttccaccttt aacacgtgcc atgtataatt
     1081 cctcctaata aaaataaatc ttcagattct gttatttgta cttcttattt catttttgct
     1141 agttgttggc gaatacgttt gaagtcgcca ctagaaacca tgcttgcttt acgcaattga
     1201 cggcgttgtt tttttgtttt gccgtggaaa cggtgacttg taaacgcgcg gaagcgtttc
     1261 aatccgcctt taccagtacg tttgaaacgt tttgctgatc cgcggtgtgt tttttgtttt
     1321 ggcatgacta tatttcctcc tcaaaatctt tcgttatccg actatcggct gtccaacagc
     1381 cagttagtca ttcaaattac ttactgtcgt ttttcggtgc cagcgtcaag aacatgctgc
     1441 gtccgtccat tttcgctttt tgttccactg ttgcaatatc cgcagtttct tcagctaagc
     1501 gattcaagac tttttgacca atttctttat gggtaatggc acggcctttg aaacggatag
     1561 aagctttcac tttatcgccc ttttccaaga acttacgtgc attacgaagt tttgtattga
     1621 agtcattcaa gtcgatcgta ggacttaagc gaacttcttt tacattgatc actttttgtt
     1681 ttttacgcgc ttcgcggtct ttcttttgtt gttcgaaacg gtatttaccg tagtccatga
     1741 ttcgcgcaac tggtggtttc gcaccagggg caactaacac aagatccaag tttgcttgtt
     1801 ctgcaatatt caatgcttct gcttttgtct taactcctaa ttgctcacca tctgaaccga
     1861 tcaatcgtaa ctctcgtgca cgaatgccgt cgttaaccat catatccttt gctatggtca
     1921 ttcacctcca aatattatga gagaaagaaa agcttaccga ataaacggca ataaaaaaac
     1981 gagttctctt acagaacccg cgcgtacttc acctaataga accagtgaaa actatctagc
     2041 ccagtgacat tatcaactaa ggcgagaagc gggtgcttct gcttttattt ctcaaccatt
     2101 atagtatagc aaaaactaat ctttttgtca atgtttttca tacagtttgt cttccttttc
     2161 tgaaaatgat tagattagca ggctttctag cagtgattcc cgcttctttg ctatactaaa
     2221 gaaaaacgag gtgaacccac atgaaaaaaa tcgaacctat tccaccttct cttcctcttc
     2281 ttacaaaggg agagttttat ttagaagatg aggagatctt ctctggtctt ctgatcaacg
     2341 gaggtaacca aagctatctc gatgccaaaa atatggtatt aagagagagt catatccaaa
     2401 aacttacgat gcaaaaaaca ggtttacgtc attttgaatg cagtaatgtc atttttgaaa
     2461 aatgtgattt ttccaatctg gagtggattg gtggcagttt ccaccaagtc gtgtttcatc
     2521 aatgtaaatt aacaggtacg aattttgccg aaagttatct tcgagactgt actttcactg
     2581 attgtattgc ttcgatggct tcctttagta gtacaaactt gaaagctgtc tccttcgatc
     2641 attgtcaatt agaagacagc gaattttatg aggtcacttg gaaaaacctt tttttgtctg
     2701 acaatcagtt aaccggttcg aactggtttc gaacttctct gaagggatta gactttacga
     2761 ctaatttctt cacaacaatc gctttatctc aagattcttt ggcgggacta atcgtcaatc
     2821 aagagcaagc acttgtgatc gcccaagccc ttggtttgca gatcaaagac tagatcaacg
     2881 aatccgccgc cacagtgcaa taagcatcca aatagataag accaaactga tgatcaaggc
     2941 gatttcccat ccgaaaacgg aatgggtaaa gggtaactgg agattcattc cgaagaatcc
     3001 tgtcacaata gttggaacgg tcaagatcag cgaccagacc gtcaaaaatt tcatcgtgtc
     3061 attcaagttg ttattcagca aattattata agtgccagag agctggtcta aaatctgcga
     3121 agctaagttc gtcatttcaa ctgcttgtcg tgcttcgatc agtgcatcgt ccaactcttc
     3181 tttttccttt tctgacaact tgcgataaat agccagtgcc ttgatttgtt ctaaaagtac
     3241 tacattttgt tttgttcctg taactaagta aaccaaaccg atctctaaat ctgaaagttg
     3301 cagcagattc ttgttcgttg ttttttctct taatttcaga ttaagccgct gacgttcact
     3361 attcacttcc tcgattaaag gaaaaaagga atcagaaatc aaaaacaatg tacgaaacaa
     3421 taaactgtat acactcatgt ctggcatccg ttccaaaagc gatcgaatta gtcgaatcac
     3481 gtactcggta ttcggcgtgg taaatgtaaa caaaccgtct tcttttaaaa taaaagcgat
     3541 aggactagtt tcataatgat tatcgacttt ttgctggtgc ggaacatttg atacaagcag
     3601 taacgcttca tcaaaagaat cgtactctac gcgcgcccgt tcatttttat ctagagaata
     3661 agtcagcatc tcgtcactaa tgccatattc ttcttttaag tttttaacag ctagcacatc
     3721 gttttgctgt acattgatcc aaaaattacg gtcttgatta aaatagtgtt tttcccgcat
     3781 actctatcct cctgacattt gtttccagta taaagaaaaa agtcgctcaa ataaaggttt
     3841 accctttatt tgagcaactg taaatcaaat ggatactttg gtttggcgaa tctctgctaa
     3901 tttttcttcg atcattttca agacttcttc ttttgcttga ggatcttcca caaaattata
     3961 gcggtctcca tcgatttgga tttttggact ttctttgtat tcttcatacc attgatcata
     4021 acgtctgttc aattctttgt aataatcgta tagacttggg tcaaaggaca attgttcata
     4081 cgaacgacca cgtttttcga tacgctctaa catcgtatca aaagatacac gaatatgaac
     4141 taataaatcc ggatgttttt tatgggcagc atagggaagt tcttccatca tgttttccag
     4201 cagttcatca tacacacaaa cttctgtttc tgttgctctt cctagatctg catttagatg
     4261 gaaaagcagt gaatcttcat aaatcgaacg gtccaataca ttattgtcat ctttcattgc
     4321 ttgtttgatg cttgcaaaac gtttattcaa aaaataaatt tgcagcaaaa aagcatactg
     4381 ttcagggttt gcataaaata agggcaagac ttcattatca tcaatcgatt cataaaatgc
     4441 ctgactatca aagtgttcag ccatcatttc tgttaaactt gattttccgg caccaattgt
     4501 acctgctaaa acgatcacgc tcatttacct ccagctcact tcatcactaa atattgtgtt
     4561 taactccaac cttttttaaa tataccccca atatctagta gtgtcaattg tattttttct
     4621 agtcaaagaa gaaaaaaagt caaaaaattc ttaagaaaaa agtgtttttt ctttagaaaa
     4681 cgtattctta gttttacttc tgagatggtt ttagtaaaat aaaggaggaa tgttttttga
     4741 gacccttagt ggacaaaaaa attcgtgaag cagcgcaact cgttttgggc gctttatttt
     4801 ttgcttaaaa atcactttta cgtgagaagg agttagatta tggcaaacta tggccaagga
     4861 gaatcaagcg gaaagatcat attgatgggc gagcacgcgg ttgtttatgg agaaccagcg
     4921 attgcctttc ctttctatgc aacaaaagtc accgcattcc ttgaagagct ggatgcaatg
     4981 gacgatcaac tggtttcttc ctactattca ggaaatttag ccgaagctcc tcatgcatta
     5041 aaaaatatca aaaaattatt cattcactta aaaaaacagc atgacatcca aaaaaacttg
     5101 caactgacca ttgaaagcac gattcctgct gaacgtggaa tgggatcaag cgctgcagtc
     5161 gccacagcag tcactcgtgc tttttatgat tacttagcat ttcctttgtc tcgtgaaata
     5221 ctattagaaa atgtccagct ttcggaaaaa atcgcccacg gtaatcctag tggaatcgat
     5281 gcagccgcta ctagcagctt gcagccgatt tattttacaa aagggcatcc ttttgactac
     5341 ttttctttga acatcgatgc ttttttgatt gtcgctgata caggaatcaa aggacaaaca
     5401 agagaagccg tcaaagatgt cgctcacctc tttgaaaccc agcctcatga aactggacaa
     5461 atgatccaaa aattaggata cttgacgaag caagcaaaac aagcaatcat tgagaattca
     5521 ccagaaacgt tggcacagac aatggatgaa tcacagtcac ttctggaaaa gctgacaatc
     5581 agcaatgatt ttcttaatct attgatccaa acagcaaaag ataccggggc attgggtgcc
     5641 aaattaactg gcggtggacg cggtggctgt atgattgcat tagcacaaac aaaaacaaaa
     5701 gcccaagaaa tcagtcaagc acttgaagat gcgggtgctg ctgaaacttg gatacaagga
     5761 ttaggagtac atacctatgt ttaaaggcaa agcacgcgca tataccaaca ttgctctaat
     5821 caaatattgg ggtaagaaaa atgaagaact catccttcca atgaacaaca gcctttcgtt
     5881 aacattggat gcattttata cagagacaga agtcatcttt tctgatagct atatggtaga
     5941 tgaattttat ctagatggca ccttacaaga cgaaaaagca acaaaaaaag tcagtcagtt
     6001 tcttgacctt tttcgtaaag aagccggcct ctcactaaaa gcttcagtaa tcagtcagaa
     6061 tttcgttcct accgcagctg gattagcctc ctctgccagc gggctagctg ctttagcagg
     6121 agcttgcaat actgctctta agcttggatt agacgatctc tctctttcaa gatttgctcg
     6181 acgcgggtct ggttcagctt gccgaagtat tttcggtggt ttcgttgaat gggaaaaagg
     6241 ccatgacgac ttaagttctt acgctaagcc agtcccttcc gattctttcg aagacgattt
     6301 agcaatggtt ttcgttttga tcaacgacca gaaaaaagaa gtgtccagca gaaatgggat
     6361 gcgtcgaaca gtcgaaacat ccagttttta tcaaggctgg ttagattccg ttgaagggga
     6421 tctatatcaa ttgaaacaag caatcaaaac aaaagatttc caacttctcg gagaaacgat
     6481 ggaaagaaac ggactaaaaa tgcatggaac gactttagct gcccagcctc cattcactta
     6541 ctggtctcct gacagcttaa aagcgatgga tgcggtgcgg caattgagaa aaaaaggcat
     6601 tccatgctac tttacgatgg atgctggccc taatgtcaaa gttttagtgg aaaacagcca
     6661 tttatctgag gtacaagaaa catttaccaa attatttagc aaagaacaag taattactgc
     6721 ccatgctggt ccaggaattg cgattattga ataaagggag atttttatga ttgaagtatc
     6781 tgcaccaggc aaactctata ttgccggaga atatgcagtt gttgaaacag gacatccagc
     6841 agttatcgct gcagtcgatc aattcgtaac agtcactgta gaatccgcac gaaaagtcgg
     6901 aagtatccaa tctgcacaat atagtgggat gcctgtacgt tggacaagac gcaacggaga
     6961 attagtttta gatattcggg aaaatccttt tcattatatc cttgctgcta ttcgtttgac
     7021 tgaaaagtat gcacaagaaa aaaacattct tttatccttt tatgatctga aagtaacaag
     7081 tgagttagac agttcaaatg gccggaaata tggattgggt tctagcggtg ccgtgaccgt
     7141 tgcaacggtc aaagccttga atgtttttta cgcgttgaat ttatctcaat tggagatttt
     7201 caagattgca gcactagcca atttagcagt tcaagataat ggttcctgcg gcgacatcgc
     7261 tgctagctgt tatggtggct ggatcgcttt ctcaaccttc gaccatcctt ggctccaaga
     7321 acaagaaact cagcattcta tcagtgagtt acttgccctg gattggccag gtctatccat
     7381 tgagccattg attgctcctg aagatttacg tttattgatt ggttggacgg gtagccctgc
     7441 ctctacttct gatttggtcg atcaagttca ccgttcgaga gaagacaaaa tggtggctta
     7501 tcagcttttc ttaaaaaaca gtacagaatg tgtcaacgaa atgatcaaag ggtttaaaga
     7561 aaataatgta acgttgatcc aacagatgat tcgaaaaaac cgacaattac tgcatgattt
     7621 atctgcaatc actggggtcg tcatcgaaac gcctgctttg aacaaattgt gtaatttagc
     7681 tgaacagtat gaaggagccg caaaatcttc tggtgcaggt ggtggcgatt gcggaatcgt
     7741 aattgttgac cagaaatctg gcatccttcc tttaatgagt gcatgggaaa aagcagaaat
     7801 cactccactg ccgttacatg tctatagcga tcaaagaaag gaaaaccgat gaatcgaaaa
     7861 gacgaacatg tttcactagc taaagccttt catgataaac aaaaaaacga atttgacttc
     7921 gtacggatca tccataaccc tttgccgcaa atagcagttt ctgatgtcga tctaagtaca
     7981 caggcagttg ggtttacact aagcagccca ttttatatca atgcgatgac tggcggaagc
     8041 gaaaaaacaa aaaaaatcaa ccaagatctt gcaatcgtcg caagagaagc agatttgatg
     8101 attgccactg gttcagtcag cgctgccttg aaagaccctt ctttagccga tacttatacg
     8161 attatgcgac aagaatatcc tcacgggaaa atcattgcca acatcggtgc agggacttct
     8221 gtcgaaagag cccaagaagc tatccgattg tttcacgcag atgccttaca gattcatttg
     8281 aatgcacctc aagagttggt tatgcctgag ggagaccgtg attttaccaa ttggaaagta
     8341 cttattcaag agactcaaac agccattgac gtccccctca tcgtaaaaga agtcggtttt
     8401 ggcatgacaa gagaaacgct caatgatcta gctgctttag gagttcatac agttgatatc
     8461 agcggtcgaa gcggaacaag cttcacccag atcgaaaacg cccgtcgctc caagcgggaa
     8521 ctgagttatt tagctgactg ggggcaatcg acagtctctt cattgcttga agcaaatgaa
     8581 gcagacactt ctatggaaat cctagcctcc ggaggtatcc gcaatgccta tgacatattt
     8641 aaagcccttt gtctcggcgc aaatgcagtg ggtacttcag gaaccgtact gacgcatctg
     8701 atgaatcacg gcgtagaaga aactattata ctgatgaagc aatggcaaga agagcttcgt
     8761 ctgctttata ctatggtcgg cgcaacgaat accgcggccc tacaccaaca atcactgatc
     8821 ttttccggac cagtgaagga ttggtgcgaa gcaagaggga tcgacttagt gaagtatgga
     8881 agacgcacag aaaagagcgt gggacaaaag taaagaacac ttttatccca cgctctaaac
     8941 acgtataaac ggcggaagca aaagcaactc cttcggaaat aagctgaaat tcacaaaaat
     9001 ttgaaaagca attttcgtga attccttctt atttcttgga gttaaacgct tctgtcccag
     9061 cctctttttt cgaataaatg atgtttaatc agctgtcctt cggtattcgt tcaagccgct
     9121 gatttcacaa cctctccact aaacggtgta ctaaagctct tttaaggttg ttttccgata
     9181 taggcaagta tgcctccgtc cacatacaag acatggccgt taacgaaatc agatgcgcta
     9241 gaagccaaga agacagatgg tccagccaaa tcgattggct cgccccagcg tgctgctggc
     9301 gtacgtccaa cgataaattg atcgaatgga tgacgttcac cgttttcttg tgtttcgcgt
     9361 aatggagcag tttgcggtgt agcaatataa cctggtccaa ttccattaca ttggatattg
     9421 tattgaccgt attcagaagc gatgtttttc gtcagcattt ttaatccgcc ttttgccgca
     9481 gcataagcac tgaccgtttc gcgtcctaat tcactcatca tggagcagat attgatgatt
     9541 ttaccgccgc ctttttcgat catatcccga ataactgctt ttgaaacgat gaatggggca
     9601 tttaaatcca cgtcgattac ttggcggaag tctgccgcat ccatttcgat catcggtgtc
     9661 cgtttgatga tccctgcatt gttgactaga atatcaactg aacctacatc ttctttgatc
     9721 tgttgaacca tcgcttgaac cgcttcttca tcggtcacat cgcagacata accttttgca
     9781 tcgatgcctg cttctttata atgcgctata ccttcatcaa ctgattcttg attcaagtta
     9841 ttaaatacga tttttgctcc tgctgtagct aaagagcgtg caatctcaaa tccaatacca
     9901 tagacagcac cggtgactaa tgctactttt ccatctaaac gaaacatatc catactaaat
     9961 tccatcattt tctctccctt atgcttattt taattgatcc atttgcacca tatccatgtc
    10021 tgtataggtg atattttctc cgcacattgc ccagatgaat gagtaattgc ttgtacctac
    10081 acctgaatgg atcgaccagc ttggcgaaat aatggcttgt tcattgtcta cgactaaatg
    10141 ttttgtttca tctggtttgc ccatcatatg gaacacacgt gtatcttcat tggcataatc
    10201 aaaatagaca taagcttcca tacgacgttc atgtgtatga caaggcatcg tattccaagc
    10261 acttcctggt tctaaaatcg tataacccat ttataaatga cagctatcgc tgaagcttct
    10321 actgctgtaa atatccccgt caagatacca ccgataatga tgacaatcaa tagaaggcta
    10381 ggaatcgcct cttttattat ttttccagca tgagccatct gtcctttttc gacaacaggg
    10441 tatttattct ttttagcaac gatgaatgct actagcatga ttcctagcgc ccacaaa
//
LOCUS       DAMEET010000085         9961 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.086, whole genome shotgun sequence.
ACCESSION   DAMEET010000085 DAMEET010000000
VERSION     DAMEET010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.086"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(184..2304)
                     /locus_tag="PB780_002117"
     CDS             complement(184..2304)
                     /locus_tag="PB780_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDE0734157.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2534..3712
                     /locus_tag="PB780_002118"
     CDS             2534..3712
                     /locus_tag="PB780_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HDE0734158.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3728..4486
                     /gene="aroD"
                     /locus_tag="PB780_002119"
     CDS             3728..4486
                     /gene="aroD"
                     /locus_tag="PB780_002119"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDE0734159.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4509..4994
                     /gene="ybaK"
                     /locus_tag="PB780_002120"
     CDS             4509..4994
                     /gene="ybaK"
                     /locus_tag="PB780_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HDE0734160.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(5065..6318)
                     /locus_tag="PB780_002121"
     CDS             complement(5065..6318)
                     /locus_tag="PB780_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDE0734161.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6435..7784
                     /gene="gdhA"
                     /locus_tag="PB780_002122"
     CDS             6435..7784
                     /gene="gdhA"
                     /locus_tag="PB780_002122"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDE0734162.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7896..9242
                     /locus_tag="PB780_002123"
     CDS             7896..9242
                     /locus_tag="PB780_002123"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDE0734163.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9550..9936)
                     /locus_tag="PB780_002124"
     CDS             complement(9550..9936)
                     /locus_tag="PB780_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDE0734164.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3187 a         1804 c         2038 g         2932 t
ORIGIN      
        1 ataaagtatg aaattccttt agtgaaatca cgaattaaaa ccagaaaata ttttgtaaaa
       61 agcaagaggt tgtgaagaag ctgccctatt ctattaccgt agatgcaagc ttttgaacac
      121 tgtttattcg aaaagaggag cagcgacaat aattttgtcg ctgctccttt taaatttatt
      181 ttatcattcg gaagaacttg tggtcgttcc gtgaagttct tggtacgttt gcgttttata
      241 aagatcaaca gttgattgtc cgttgtgttc actaaagaca ctagttgact tgtcaccaag
      301 agatttttca gcttctttta atttctctaa gccattcttg tagtcataat cactggtatc
      361 aattggatct aatccactat tcgtatagaa ccgaagaaga tcaccattat tgatttggtc
      421 agaaacagct aactgtttat caactttttc tttcagtgaa tcaatttcat tctgtacttc
      481 ctcagtaggt tcagtaatag cttcaccagt ttcgttacta tagatcgtac caccgtagta
      541 agtatatttt ggtgtaacaa aatcaccatc acggaaagaa acaagctgtt catgatcttt
      601 cgagaataga tcttgtccta actgaatata attttttgta tctacaccta gtaagtgaag
      661 taatgtaggc aaggcatcaa cttgtccgcc gtaagtgtga ttgattccgc cctgtgtttg
      721 tccagggata tggatcatat aagggacacg ctgcatttgt gcattgtcgt agtttgtcca
      781 agtagaactt gtttttccaa ctaattcagc tagattttta tttcttgaat tcgagacacc
      841 gtaatggtca ccgtaaagga cgatgactga attatcatac aagccgcttt ctttcaaata
      901 attgaagaat tcttgtacgg cggtatctaa ataattggct gtcgcaaagt aaccgttgat
      961 cgtttcatca tttgtattgg ctaacgggaa tcctgcttcg tcattggtga attttgaata
     1021 aggataatgg ttagatacag caatgaattt tgaataaaat ggttgttgca gacgttccaa
     1081 gtattgtacg gattgttgga aaaatggttt atcatgcaac ccgtattgga acgaattgtc
     1141 atcattaaca tcataataag aagaatcaaa gaaataatca tatcctaaat tcttgtatgt
     1201 ctcgtttcga ttccagaatg taccagcgtt cccatggaat accgcactaa tgtaaccttg
     1261 tgtttgactt aaaatatcag gtgcagcttg gaaagtattt tttccgccta attgtgtgaa
     1321 caaggagcct tgattcaagc caaacaatga attttccatc agcgtttccg catcgcttgt
     1381 ttttcctgct tttacttgat ggaaaaagtt gtcaaaactg aacgtgctgt tgctgtggta
     1441 aagactattg atgaaaggtg taacttcatg ctggagacca ttctcatctg tcaatttata
     1501 atcgatcagg aattgctgga agctttctaa atggatataa atcacatttt ttcctctagc
     1561 gagcccgaac aaatcgtcat ttggtgctgc ataatgttct tttacataat tttctacttc
     1621 tttcatatca ttgggacttg cttctgcccg cacttggttt gtctggtaag tttgaacacc
     1681 gtcataaacc atgaaagcat tcaagccaag atatttgacc aaataatcac gagagaatgt
     1741 tcgagtcagc aattctggac gatcagcttc cgccataaaa agatttcctg agaaaatcat
     1801 aactgctaat gtagagatcg caaaagccat tcttgctcgg atcgggcgag ggtccaactt
     1861 gatttttttt gcaatcagca gaacagtcaa gataataaaa tcgatccaat agatcacatc
     1921 atatgggcga aacagtcgaa tggcactttc tcccagacca ctcgctactt tcccagcacc
     1981 taaaatcgtg ttgatcgtaa tgaagtccgt gaattctcga taataagtga cattcgaaaa
     2041 aacaagcaat gtcaaaagaa aataaatgac catcatcgtg atataagcaa ctctagggcg
     2101 tctgatataa agagcaattc caagtaataa caatgtagtt gcaatagggt tgataaacaa
     2161 gataaagtat tgaatgccac tttcgattcc gagatggaaa tcaactaaat aggcaaaaat
     2221 cgttttcaac caaagtaaaa cgactagtaa agaaaaaaat ccgagcctct tattcaaaaa
     2281 ggcaggtgtt tttatacatt tcaagataat cttcctttct gcacttctta atcacagaag
     2341 tgtcctgaaa ccatgttaca cagctcactt attctcattt attttactag tatgacaata
     2401 tagtgtcaat tgaaaccaga agtttatgct gtttcattaa ggttttcttt aagcttcatg
     2461 aaaaaatgag cagtaagaca agtcctatgt tttttgttat acttgggaag agaaagctaa
     2521 agcaggtgaa ggaatgaaag taaaagtaaa aaaaagagca agcaatcgat tgaaaaaagg
     2581 atatccactg atccaaaaag aagaccttgc tcatccccaa aaagaaacca cgcaatgggt
     2641 ggaattagtt gattcgcaaa accaatttat cgcaaaagga tatctaggaa agcagaataa
     2701 gggaatcggt tgggtattga gccagcagaa catcccattt gatcaatctt tttttgaaga
     2761 attttttgtg aagtcaaaag aaaaaagaac tttctttttt tcagatgatt ctactaccgc
     2821 ttttcgtata ttcaatggag aaggagacgg gatcggcggt ctgattgttg attattatgc
     2881 aggttatgct gttttttctt ggtataacga aacgttatat gcacatcgtg aattattgat
     2941 tcaagctttt cgtgaaatct acccggaaat cataggaggc tatgagaaaa ttcgatttga
     3001 atcagtaaat ctgcctgaga gccagttcat ttacggaaaa gaggcaccgg aacccttgat
     3061 cgtaaaagaa aacggagtaa aatttgccgt ttatttaaat gaaggattaa tgactgggat
     3121 ctttttagac caaaaagaag tacggggatc attagcagaa ggtttcgctt ctgggaaaac
     3181 agttttgaac atgtttagtt ataccggcgc cttttctgtt gctgccgcaa tgggaggggc
     3241 aatcgaaaca acaagtgtcg atctagcaaa acgcagttta aaaaaaacac aggaacagtt
     3301 tgaagtgaat ggtttggatg tcgatcagca aaaaatcatt gttatggacg tattcgagta
     3361 tttcaaatac gccaaaagaa aacaattttt atatgacatg attgtattgg atccaccaag
     3421 ttttgccaga aataagaaaa aagtctttcg cgtagcaaaa gattatgggg aactggtcaa
     3481 ggatagtctg gatattttag cagaagacgg gatacttatt gcgtctacaa atgctgcgaa
     3541 tgtctcgata gaacaattcc aaacaatgat tgaagaggaa ttccatgcca aaaatgtttc
     3601 ttttgaacaa cttgctttat atcgtctgcc aaaagatttc cagacgacgg ataccttttt
     3661 agaaggcaat tatctgaaag tgtttgttta tcaaattaaa aataaccatt agaaggagcg
     3721 atgcagaatg gttcgggtaa agggattgaa tataggaagc ggtagaccaa agatttgtgc
     3781 gcctcttatc ggggccgtcc gagaggaagt attacgtgaa gcagttcttg cacgtgaagc
     3841 aggggccgat ttagtcgaat ggcgagtcga tcattatcgt gaagtttttt cttttgatga
     3901 agtagcagaa acattggttc ttatccatga gatgcttgat ggactgccgt tattatttac
     3961 gtttcgtacc ttagctgaag gcggagaaca agacatatcg atccgtcagt atcgcgacct
     4021 ttatgaacat gtgatacaca caggattagt agatatggtc gatatcgagt tgttcaaagt
     4081 agaaagtcta ggaaaaagac tgatagagga aatcaagtct ttaaacattc ctttaattat
     4141 cagtagtcat gattttaaag aaacaccagc agaccctgtc ttgctttacc gattgaatgt
     4201 tatggaacat tttggagccg atatcggaaa actggcagta actcccaata atgaacgaga
     4261 tgttttgcgg ttgatggagt tgactagacg ggcaaatgct tttgtctcca tgcctattat
     4321 tacgatgtcc atgggaaatc ttggtaaaat ttctcgtctg gttggcggaa cgacgggttc
     4381 cgttatgaca tttggttcct taagcagcga ttctacttct gctccaggac aattaccggt
     4441 caaagcattg aagcaaatta ttcaattatt aaatgaaccg ttgtagaaca agtttgaggt
     4501 gatatttttt ggcaaagaaa aaaatagtaa aaacgaatgc aatccgtatg gtcgaacaaa
     4561 aaaagattcc ctatacagag cacgaatatg agtgggacga aagccaccta agtgcgtcat
     4621 cggtcgctga acagcttcca gaaagccaat ctcgtatatt taaaacactg gttgctgtgg
     4681 gcaatgtgac ggggcctctt gttgctgtga taccgggaga agccgaattg aatctaaaaa
     4741 aattagcaaa agtttcaggg aataaaaaag tagaaatgct ccatttaaaa gatttggaag
     4801 caacgaccgg atacatacgt ggtggctgct cgcctatagg tatgaaaaaa ctatttccta
     4861 cttatttaga tcaaattgca gaaagctatg aacaaatcat tgtctcggct ggcagaagag
     4921 ggctacagat ggagttagca cctcaagata tctgtgcatt aacgagcgga caatttgcag
     4981 atataaaaca ataaatcata cgtataaaag aaagaaggag gccgggatgt tgtttgtcca
     5041 gtctccttct ttctttttta tacattatgc tctttgcccg gcattcattc ctgctacatg
     5101 ccctgtagcg aaagccgctg tgatattaaa gccgccagta taaccattga catccagtaa
     5161 ttctcccgca aaatacagac catcaatgat tttactctcc aatgttttag gataaatttc
     5221 ctttagagaa acccctccgc cagttacaaa tgatttctca ataggaaaag ttttgttgat
     5281 tgatagctta aactctttgc atagttgaac aaaatcttga atttgttttt gagaggcttg
     5341 ttgtcctgtc agatgatcca tttcaagccg ctcaagaaag aaaactaaca atcgctcagg
     5401 caatatacca tgccaagcat tgaccaagtt ctttttggtt gctttacttt tttctgtcaa
     5461 tatatgaata agctcttgtt tggtttgtgt agggaaacaa tccagagaaa gtgtcactgg
     5521 ttctcccgtt ttctctaatt ctttattgac aaaacttgaa caacgcagtg cagccggacc
     5581 agaaataccg aaatgggtga aaagtaaatc catcgtatgt tcagtcaata cacgaccttt
     5641 tttatttaaa acgcgtaaag tgatgtcttg taaagaaagc ccttgaagtg ttttctcctg
     5701 aatatacggt tcatccgata tcaaggggga ctctgtcgca taaagaggcg tcaccgtgtg
     5761 acccactcgc ttcacgatct tgtacccatc tccagtagaa ccagtagatg gataagtccg
     5821 tccaccagtt gttaagataa tggcaggagc atggaactct tcgaattccg tgcgtatacc
     5881 gtaaatttta tgttctttgt ggatcaattt ctctactcga gtagagaata gaagcgtcac
     5941 atctagttct ttcagacgat tgagcaatgc ttcgataatc gtttttgatt tatttgttac
     6001 aggaaacatc cgtccatgat cctcttcttt taaatgtacg ccttgtgatt caaagaagtt
     6061 catgatatcg aaattattcc attgcgcaaa cgtactatat aaaaactttc catttccagg
     6121 aatatgtgca atcaaatcat caacaggacg attgttcgta acattgcacc gaccaccacc
     6181 tgtcatcagt agtttttttc ctgctttttt gtttttttcg atcaagagta cgcgtgcgcc
     6241 ttgttcagct gcactgatcg ctgccatcat cccgcttgtt ccagcgccga ctacgatcac
     6301 atcatacagt ttttccattc cctcacctca aggaaagtgt agcatatctg aagaaagaaa
     6361 acagaaaaaa atgttttgag ccaaaagaga aaatgagcta aaatgaggga agataagcaa
     6421 aggagcgtgg aacgatgaca aacgcaacag aatatgttca agcaattcaa gaaaagctac
     6481 atcaaaagga tcaagggcaa gaagaatttt tacaagcaat cgatgaattc atgcccacgg
     6541 ttatgtcttt tttggacact catcccgaat atatcgaaaa gaatatctta gaacttttaa
     6601 cggaaccaga aagagttatt caatttcgag tgccttggca agatgatgaa ggaaactggc
     6661 gagtaaatag agggtaccgt atccaataca actcagctat cggcccttat aaaggtggat
     6721 tgcgcttcca tcctagtgtc aatctaagta tcttgaaatt tttggctttt gaacagattt
     6781 tcaagaatag tttaaccggt ctgccaatcg gtggaggaaa aggcggaagt gacttcgatc
     6841 ctaaaggaaa atctgataat gagatcatgc gtttttgtca aagtttcatg ctggaattgt
     6901 caaaacatat cggcccatcg attgatgttc ctgcgggaga tatcggtgta ggagctagag
     6961 aaatcggcta tttgttcggc gaatataaac gattgaaaaa atatgatact ggtgtgttga
     7021 caggtaaacc tttggatttt tggggaagta agatacgtac ggaagctact ggttatggat
     7081 tagtgtatta tgtcaaacat ctattgaacg aagaaaaaga ttcatttaat caaaaaacag
     7141 tttttgtttc cggaagcggg aatgtagcca tttatgcaat cgaaaaagtg caagagctag
     7201 gcgggaaagt tgttacatgt tcagattcta gcgggtacat ctatgatcca gaaggaatag
     7261 atgtgaaact attgaaagaa gtcaaagaaa tcaatagaca acgtttgacg gaatacgcaa
     7321 accaacgtcc ttctgctatt tatcatgctg gagaatctgt ttggacattg aaagaaaaag
     7381 cggatatcgc tttgccatgt gctactcaaa atgaaataga cgaaagcttg gccgaaatct
     7441 tagtagaaaa tgggatcaaa atcgtagcag aaggcgctaa tatgccatgt acagtaaaag
     7501 ctgtttctgt tcttcatgaa gcaggcgtgt ggtattgtcc aggaaaagct gctaatgctg
     7561 gaggagtagc agtatctgct ttagaaatga gtcagaacgt ccagcgattg acttgggaaa
     7621 gagagcaagt ggatgagcag ctggatcaaa tcatggaaac gatttaccat acgtgtcgcc
     7681 aaacagcagc cgagtacggc gacgaaaaaa atctgcttct tggagcaaat gttgctggat
     7741 ttgaaaaagt tgccaaagca atggcagcag aaggtttagt ttgaaaaatg aaactttctt
     7801 ggaaatattg tcagcatttt gtttatttgc ttgaaaaata ataaaaaaaa acgtaaagta
     7861 ttacctgtag agtaaaccta taaggaggag aacaaatggc acacattcat tttgactatt
     7921 caaaagtagc accgtttgtc aacgaacaag agttagggta catgcaaagc caagtaacag
     7981 cagcccacaa cgaattacgg gaaggtactg gcgcaggaag cgacttccgc ggctggatcg
     8041 atttaccaac gaactatgac aaagaagaat ttgcacgtat caaagaagca gctaaaaaaa
     8101 tccaatctga ttctgaagta ttagttgtta tcggtatcgg tggttcttat ttaggcgctc
     8161 gtgcagcgat cgacttcttg aatcacacat tcttcaattt attagatagc aacacaagaa
     8221 aagcaccaca aatcttcttt gccggaaatt caatcagctc aacatatatt gctgacttga
     8281 tcgaagtaat cggtgatcgc gacttttcag taaacgttat ttcaaaatct ggaacaacta
     8341 ctgaaccagc gattgcgttc cgtgtattca aagagcttct tgttaagaaa tatggacaag
     8401 aagaagcaaa caaacgtatc tacgctacaa cagataaagc aaaaggtgca gttaaagtag
     8461 aagcagatgc agaaggttgg gaaactttcg tgattcctga tgacatcggc ggacgtttct
     8521 ctgtattaac tgctgttggt ttgttaccaa tcgctgttag tggtgcagat atcgatgcat
     8581 tgatgcaagg tgccgctgat gcaagcaaag catactcaag cgacaaacta gaagaaaacg
     8641 aagcttacca atacgcagca atgcgcaaca ttctttaccg taaaggcaaa gtaactgagt
     8701 tgttgatcaa ctatgaacca ggaatgcaat acttctcaga atggtggaaa caattgtatg
     8761 gtgaatctga aggaaaagac caaaaaggca tctatccttc aagcgcaaac ttctcaactg
     8821 atttgcactc tttaggacaa ttcatccaag aaggacgtcg caatattttt gaaacagttg
     8881 tgaaggttga aaaacctcgt aaatcaatca caattcctga acaagcagaa gatttagacg
     8941 gtctaggcta cttgcaagga aaagaagtgg acttcgtaaa tacaaaagca tttgaaggaa
     9001 cattgcttgc tcatacagac ggtgacgtgc ctaacttgct agtcaaaatt ccagaaatgg
     9061 atgcttatac attgggttat acaatgtact tctttgaaat tgctgtaggt atttcaggtt
     9121 acttgaatgg aatcaaccca ttcgaccaac caggtgtaga agcttacaag aaaaacatgt
     9181 tcgcacttct aggcaaacca ggctttgaag atcttgctaa agaattgaac gaacgcctgt
     9241 aagataaggg tttttcgcta gatttaaata atcagtgata gaaacatgtt cgctttaaaa
     9301 tgaaaactgg tacccattta ggtactcaaa catactgcat cgtttattca gaataagggg
     9361 ctgtgacaag acgtttgtca cagtcccttg tttgtataga aatcgattga ttgccttgct
     9421 ctttagtttg ttttaggact ttaagaacag tgaggctgac ttcgaaggtt aattctgttt
     9481 ttttgttttc tagaatcagt aagcaaaaaa gcactcactt cgagtgcttt tttgcgatat
     9541 attcttagct taagataggc ctaatttttc ttttacagat gagggtacct tatcgctggt
     9601 aattactttt ttctctatga tccgcttttc gcttgctttc aactgcaaga tcgttccttc
     9661 ggggtaagca gaaccattgg cagagtcacc agaagtaatg acatcgaaat ctaactgtct
     9721 tttttcaccg ttttcagcat aagcatctac gttgtattta aaacctcttc caattacttc
     9781 cccattatct gctttgatat cagtttctgc aggcattgga gctttgacta ccgcataata
     9841 atctttacct acataagtag actgatagta gtcccaacct ttccatccta taaaagcaat
     9901 caatatgata acgatcaacc ctaaaatttt tttcatgaaa gatctccttt tttattttta
     9961 t
//
LOCUS       DAMEET010000086         9933 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.087, whole genome shotgun sequence.
ACCESSION   DAMEET010000086 DAMEET010000000
VERSION     DAMEET010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.087"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            205..684
                     /locus_tag="PB780_002125"
     CDS             205..684
                     /locus_tag="PB780_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734165.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            717..2945
                     /locus_tag="PB780_002126"
     CDS             717..2945
                     /locus_tag="PB780_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDE0734166.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3074..4717
                     /locus_tag="PB780_002127"
     CDS             3074..4717
                     /locus_tag="PB780_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDE0734167.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4790..5335)
                     /locus_tag="PB780_002128"
     CDS             complement(4790..5335)
                     /locus_tag="PB780_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HDE0734168.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5358..5843)
                     /locus_tag="PB780_002129"
     CDS             complement(5358..5843)
                     /locus_tag="PB780_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDE0734169.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5833..6645)
                     /locus_tag="PB780_002130"
     CDS             complement(5833..6645)
                     /locus_tag="PB780_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HDE0734170.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6784..8316)
                     /locus_tag="PB780_002131"
     CDS             complement(6784..8316)
                     /locus_tag="PB780_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HDE0734171.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIISLSPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8425..9456)
                     /locus_tag="PB780_002132"
     CDS             complement(8425..9456)
                     /locus_tag="PB780_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDE0734172.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3302 a         1835 c         1911 g         2885 t
ORIGIN      
        1 aaatattcca acatcatctt ggactttcta catgttacta atgagcgtgt aaaagaacga
       61 gatggtgttt ttatttctat caatgaagct ctttttcttt gcaatcctca cttcaatcct
      121 ctataatgtc gcatatagtt tagtaaatga gtaaactact aatttactta tttactaaaa
      181 ataaaaggat gaggagttaa aataatggaa ataccaacaa atttaaaaca taaaccagta
      241 atcgttgtag aagactatga taaagttgat ggaagaaatg cattaaatat ggatgcaaaa
      301 gggctttcac tgggccttgc tcagtggaat gatcgtggaa aagtcgatat ctcaggcaag
      361 gtatggcgac atactggaga aaaatggtca cgacagtcgg aagaattacc aatcacacgt
      421 attttagatt tagcgatctt agctgcgcaa ggaagtttat acttccaaga tgcttatcgt
      481 tatgaaaaat tttatgatcc agaaaatcca gtcgtagaca tcattggatt acaagggaac
      541 cgaatgacgg tagaagtaga taccaaaaat ccaaagatcg atgaagacat cattttgtat
      601 tacgatacac tacaaaaaga cggagaactg attggggaac gattccgtat tttaaaacgt
      661 cttttagatg agctaggata ttaatagaca gagatgatca aaaggggaag agaagcatgg
      721 agaatttctc actaacagaa aaccgaacct tttcacggct agaaaagcaa atgatctgga
      781 agaaaccgaa aagccatcgg acaagtgcag aagaatggcg gatcgcctcc gaaatcaaag
      841 ggaattggca agatcctgaa atgaaaatca gtaacgtact tttggaagga gatgccgggt
      901 cggggaagac acaattagcc aaagcactat ctgctgatct gcaacttccc tatacaaaag
      961 tgacctgttt tgcagatatg gacaaatcag atgttttcgg agccttatta cctgtagtag
     1021 attctaataa ggaacaagac caagaattac tagaagctat ttaccaaaca gatacacttg
     1081 aagctgtact gacactgatc cagaaccatt acggcattga tcctttttct gctaaagaaa
     1141 aattagcaga gctagttcaa aggatcgaag ctgattatcc agatgcaatc cattacagct
     1201 attacccttc tgaaatcgtt cgagcgattg aaaaaggcta tttattagag attcaagagc
     1261 cgactgtcat ccgagatgcc tctgttcttg tggcacttaa ctcagcgctc gaaccaaatg
     1321 ggatgttgaa tttaccgaca ggaatcattc gtcgccatcc agattgcgtc attgtgatca
     1381 ctacaaatcg aaattaccaa ggaaatcgcc cattaaatga atctttaaga gatcggatgc
     1441 aacatgcgga aaaaatggat cttcctgcct tagaagtcat ggcagaacgt gcgatggcga
     1501 aaactatgat ccaaaaacaa gaacttctct taaagatggc agagattatc cgtttgttgg
     1561 atgaaactgc aaaagctaac gccatcaaag gtgtagcagg aatgcgttct tatttttatt
     1621 gggcaaatac ctataagcaa ggacaagatt tactccagtc gatttatcca aaagttttgt
     1681 ataaattatc cactgattca gatgaattgg caattcttga acaagctttg tctgacagtg
     1741 gattgctact ggaacttcag gacatattga gaaaagaaaa atggggtcat cctcaatctg
     1801 agcatacaaa agaaagaacc attagcgccg aagaagcaaa tgaacgaaac atcactgaaa
     1861 atgctgaaga aattctgcgc ttagatgaaa agaaatcgtt gtctgctgaa gaaccaaatg
     1921 agggagaaga gaaacaagaa gaaacaatag aaaaaaacga gattcctcct caaatgcaaa
     1981 aagaacgcag tagcgatatc gaaggaaaaa atcaaaaaga acaacaaaca ggagatgatt
     2041 ctcaagaaat gtctgcttct gacatggaag cacatgataa ggaaatcaag aagcaactga
     2101 ataaagaagc gcgacaaatc atgaaggaaa cgattcatga aaaagaaggt ctcatcgttc
     2161 accgaccagc tttccaagga aaaacgccgg aagcacgtga gctacacatg caagtaatgc
     2221 caatagtaga atcacttagc cggcaaattt tagagttact ggataatgaa caaacagaga
     2281 cgtaccaaaa aggaaaatac gaaggtcagc ggttcaatgc aagcaaagta gcatatggtg
     2341 atttgagaaa ttttgacaaa aagaacccgc cacatgagca accttcttta gcggtagctg
     2401 tacggatcga tgaatctggg tcaatgatac gggatgatcg gatcgaagcg gcaaaaaaag
     2461 cagcgattgc cattgccgaa ttttctaaga aagtcgatat cccattgttg atttacggag
     2521 atacagcaga ccgttcgtca agagaaaaga cttctttgtt ttcttataaa gaatttgaag
     2581 acggatttca atggttaaat gaaaaattag tcactatgaa accaaggcaa aacaatcgtg
     2641 atggtgctgc acttcgttta gcagcagaaa aattaaacag acaatccgca acaaccaaac
     2701 tgttgcttaa tattagtgac ggtcagccaa aagcgttgcc ggattataca ggcaaaaaag
     2761 caaaagaaga tatccagtct gttttgaaag aatatgaacg tcaaggaatc ctatttgtct
     2821 ccgcagctat tggccaagac aaagaagaaa tcaagtcgat ctacggtgaa agtcgttttg
     2881 tagatattac tgacttgaat gaatttccca agcaaatgat tcaactgatt gcccgctatc
     2941 tatgataaaa taaagctcag cgaacgcact tagcagattc gttgagcttt tcgtttctag
     3001 ttaccggtaa caaggatact tctaaaatag aatcggttat aatcaagagg gaccgttaaa
     3061 ggagtgaaac gatatgatac aagaacaaaa atggtggaaa gaagcagtag gctatcaaat
     3121 ctatcctgct agtttcaaag atagcaatgg cgatggtcaa ggagacatca atgggatccg
     3181 tgaaaaactt aattatttga aagatcttgg tatcggcttt atctggatca atccgatcta
     3241 tcaatctcca tttgtcgata atggttatga cgtaagtgac tatcaagata tagaagagag
     3301 attcggaacg atggaagatt ttgatttatt gctaaaagaa gcacatgaat tagggatcaa
     3361 agtgattatg gatcttgtca tcaaccatag ttcggatcag catcaatggt ttgaagaatc
     3421 gaaaaaatct aaagaaagcc cgtatcgcga ttattacatt tgggtcgacg gggtagatgg
     3481 caaagaaccg aataattgga catcgatttt cggcggttct gcttgggagt attctcatga
     3541 aactggacag tattatctgc atgtttttgc aaaagaacaa ccagacttaa attgggaaag
     3601 cgagaaattg aaagaagaac tattcaatat gatccgatgg tggttggata aagggataga
     3661 tggatttcgt ttagatgcga tttctcatgt aaaaaaagat gaatattcag taaaagccac
     3721 agagaaccct ttctcaccct ttcaaaatgt ctcaggaatc gaagaacatt tgaccgattt
     3781 gaaacatgtt tttgaagagt acgatatcat gactgtcgga gaagcaagtg gtgtcactgc
     3841 agaagaagga ccacaatggg tagggaaaga cggttatttc gatatgatat tcgaatttga
     3901 tcatatccat atctggcagc aagaaaaaga agggcaatta gatgtcctta aattgaaaca
     3961 tgcattaagc gcttggcaaa cttcattgga tggtatcgga tggaacgctt tatatatgga
     4021 aaaccatgat gttcctcgtg ccgtttcagt gtttggagat actcgtcccg atttttgggc
     4081 tatgtctgca aaagcaatcg ctatgatgta tttctttttg caaggtacgc catttattta
     4141 tcaaggacag gaaatcggaa tgacgaatat gccctttgaa tcgatcgatc aagttgatgc
     4201 agtagattcg aaaagacttt ataaaaggct gcttgcggaa ggtaagacaa gagaagaagc
     4261 attggatatc attcgtgaaa caacgagaga caacagcagg acacccatgc aatggacttc
     4321 ggaacaatat gctggttttt ctacacatga accttggctg atcactaatc caaataccaa
     4381 aacgatcaat gtagaacaac aagaatatga accagagtcg gtcctgcaat tctataagaa
     4441 catgatccgc attagacaaa ccaataaagg tctgatatac ggttcttata aagaatatct
     4501 tcatgaacat ccacaacttt atgtctatga aagatattta gaagatgaac aatatcttat
     4561 tatggtaaac ttaacagaat cacttgctga ctatgaactt ccaaaagaag cagatcagtc
     4621 ctggacatta ctcttgtcaa attcctctag cggagaattt gaagctaaag gcatactagc
     4681 accttatgaa gctcggttat ataaaacaaa caagtaaaac ttcctcgtta aagttcatta
     4741 gttggacttt gcgaggaagt ttttcagatt gctctgtcaa ttactcattt caataattcg
     4801 ctctaggacc accgatatat ttaggacgag agcgaagggc tgtcacgtga gcttgtccaa
     4861 gggttttcaa acgaggtctg acaggtactt ggacatgctt gatatgcata ccgattgatg
     4921 tgtcaccgat atcgataccg gcttgtccaa ctacgtgttc cacttcgaca ggatcattga
     4981 actggtcaaa agcagcaaca gaacaagcac ctccagcatg tagtgcagga acaacagaga
     5041 caatttcgaa attttttgct tctgccactg ttcgttctac tactaatgct cggttcaaat
     5101 gttcacaacc ttggacagct agcgcaattt ctttaggatc caacaactct tttaacgtac
     5161 ggatgatcca ttgaccaact tccgcactgg aattttttcc aatatgtcct ccaacgactt
     5221 cgcttgtgct gcatcctaaa acaaaaagat cacctttttt caagtgtgcc tcagccaata
     5281 tgtcattaac tatttcagtc aattggtctt ttaattcttt ttctgacaac ttcattttct
     5341 aaaaccttct ttttcaatta atattgcttc cgtgcttgaa tacgttgaag acttgcagtt
     5401 ataggaaagg caagtcccat tccaatcaga ttttggaaga tgtttcctgg tatagaagcc
     5461 actccagccg gccaatcata taaaatccaa ccggcgatac catagccgat gatcataact
     5521 aagcttccta ataaaagccc taatccccac atacgagtag aagatttgct gaaagaacca
     5581 gcaacatatc cttgtaaccc gtggatcact aatgaaaaaa ctgcccactg cggatatcct
     5641 gaaatcaaat cgatcaatag accactggaa gcaccaacaa ctaatccacc cgttggacca
     5701 taaagaaaag cagcggtata gatcccggcc tcgcataatg tcacaaatcc attcgtagcc
     5761 ggtacaggga tcaaaattgt taatgaaagg gcaactgtca acgcaatcag catagcatag
     5821 atcgttaatt gcttagtttt catttctcat tcctcctttt tgaataccgc cagaccagac
     5881 acttccggta tgatcgttca tcagcacgcc attagaaata caatgggcaa caaatttttt
     5941 actttctgct actgctttgg taaaatctgc ccctaacaca agattcgctg ttattgcaga
     6001 agaaaaggca caaccggccc cgtttactgt tgtagtgtcg atttttttat gagaaaatac
     6061 ctcataatga tcaattgaat agagaagatc cacagcttca tctccagcca tccttgtgcc
     6121 gcctttgatc acaatgtttt ttgttcctga agcagtgagt tctttagcga gatggatcat
     6181 ttctgttaac gaagagggaa gagcttgacc agaaagtaaa gcagcttctg ccaaattagg
     6241 cgtaatcatc gtaacaagtg gaaaaagttc ttcgatgagc tgcgaaatat acaattgatt
     6301 gatcacagct tcagtttctt tgaatgccaa aacagggtct aaaatgattg ggatattttg
     6361 ttttgttttt atgaagtctc gaacaaccat gattgcttca cttgaattca ataacccaat
     6421 tttgatacca tctaatgaag tcattttatc aatcgaaact aactgtcgtt taagcaattc
     6481 caaagatata gattccagca taaatcgctc attaatatct agtgccccta tagaagtcaa
     6541 ggcgcttagt ccgaatagac caaaattctc aaaagttttt aaatctgttt ggattcctcc
     6601 accagcaaat ggatcagaac cgccaattgt caagattctt ttcataacat tttcctccat
     6661 gaataagagt ataacgaaat tttcaaaaaa gaaaaacagc caattggcat atttttatct
     6721 catccaattt aaaaaaacaa aaagaaaaga aagaccaaaa tggatctttc tttccttaaa
     6781 tagttaggac ttttgtgatt ttaattcttt ctcgatttga agccgttgca ctttcattgt
     6841 agctgttcgc gggattgcat caaaatcacg gatgataggg acgttcaagc gaggaagttc
     6901 atggacttgc tcccaccatc gattccaatc catttcttta tcaggagata aagaaataat
     6961 cggttgtgga gaatgatttt tatcccttac aatcactact tctgccaaaa aatctaacga
     7021 atctaataaa aagtcttcaa tagctaatgt actattgatc gtttcgatca aatccacttg
     7081 ccgatctttc aaaaataggt ggccatgctt atccataaaa ccgtaatctc cgctatccca
     7141 ccaatcgcca taaacatttt cctggaaccg tgcatcttcc ttgtaataag tcaaagcccg
     7201 tcctttagac agtaattgta tatgaccatc tgtgttttca ggtaggacat tcccaactga
     7261 atctgtaatc cgagcgcttg tcatgtcttc cagtccaacg cccatatctc ttgcatcact
     7321 tgttttcaaa ctctctagtg tatgagcctt aagaatcatt ggtccgcatt cactttggcc
     7381 atatacttga agaaataccg catcctgtgc catcgacgtt cgcaaaaatg tcttcattgt
     7441 ctggttgttt atagcatcaa aagtggagtg atagtatcgg atacccgaaa aagcttctgg
     7501 atgctcctta gcagtaaaac gccactgaac aaagttattc ggatgggtct ctagtgcaat
     7561 tggccggtga gcttccaaca tatgaacaac ttttgaactt tgtgcgtttg ctaaaggcat
     7621 cataggaaat cccatggaca tcaaagaaga gataccaata ttgaatctgg aatgcactgg
     7681 agagatgtga aaagcgatta atttcttttc cgaaattttt gtgaaaactg ttttttgcca
     7741 tttcgtccgc catcccatag aatgagcaga atgacaaata agtttaggga taccagttgt
     7801 accagaagta tgggtcatat aagaaatttc atttttgtct aaaaatgatt gagaaacaga
     7861 caaagcaggt tgttccatta aataaagaat aggaatctgt tttgattttg ttccatttga
     7921 aatttctctc actcgctttt ttgtctcatc atcaaataaa atgaaagggt cttctaaacg
     7981 atcgataaat acttccattg ttgttgttgg tagatgataa gaagtcatga caggcactgc
     8041 accaaggtaa gacacagaaa cagctagaag gtatgtgtca aaagcggaac tcttataaat
     8101 aataactttg tctccggatt tcactccaag atgagccaat tgataagccc gtttcaacac
     8161 actttcgcag cattttttgt aagttgtttc aagtcctaat tctgggaaag ctgaaagtgg
     8221 ctcatcaaag ataatcgggg tctcggaaaa gttagctgca gcttcttgaa aattcgtaaa
     8281 taagttcaaa ggggaatacg ttatggtctc catcattcac actccattat cttttgttaa
     8341 gtaatataca aattcaagat aatcgtaaca taatccttta tataataaca tagtttattg
     8401 tgaaaaaaag atcatacatt tgttttatat ggaagaaacc cgaacgaatg ccgctaattc
     8461 gtgataagaa aaatcaacca tcttttccca gatatcttga aaagaatggg atgagaggta
     8521 tcgattacca cataaataat ttaccgcgtc agcatcatct aaatatagtt ctgcataata
     8581 acgatatgtt tttctgcata gttctggcga tacgttttta tatactgtaa ttttgctcaa
     8641 aatttggcga atagtccgag gagaaatttg ctgatcatgt gaggaacgaa aaacaaaaga
     8701 attttcgtcc gtattttgtg ctgttaactt aaaatagttc tccagaaatg gcatcgcttc
     8761 ttttggtatt ggtactttat tcagtctgtt ttcttttcta ttcagattaa gaatctgctt
     8821 ggtcaaagat acatctttac gtttaagttc tactacttca tggatcttta atccgcaata
     8881 cagaagaatg gcagtaattg caaggttcct cggtctattc ttttcttcaa agctagcttc
     8941 tctgcttgtt tgaaagcctt caatcgggat aaacaaatgc tcaaaatctt tgaaaagttc
     9001 tgcatcgata tatcgcggag ctgctttggt tgtttccggt aaacttgtac ccaaaagaga
     9061 aatctcttct tcaatcagtc ctttttgata caaaaatcgt aaaaaatgat ttaatgaata
     9121 ttgcttcctt gaaatcccgt aatagctatt ttcaaacaat tgctccgttt cttggttcgc
     9181 tgtagtttgg aacgaacgag tatgtcgata gattttagct aagaaattct gataatcttg
     9241 tttttctaaa tggataatca gctgatcatc cacttcttca gaaaatactt ttctcaaata
     9301 atcagtaaac agatgaaaat cttttgcgta ttcatagcga ctgcctaatg aaagggaaga
     9361 acgattttct tctaaaaact gttgaaagac ggaagggagc aagtgtgtac gttgctttaa
     9421 cttttcttgc agttgttttc tagttttgct gtccattaca aattccctcc aatagtgttc
     9481 attttctata ttttaccgca tttcatcaaa tgaagataga cagtttgtct tgatcttagg
     9541 aagcaaattt ttaactaagt actagctaaa agatatgaag taagaagata catttttagt
     9601 tattttatta gtgcagtcat tcttttgatc ttttaattga tttatcctct ttttagctat
     9661 gtggttactt catgagcaat gaaatggatt tggttcgttc taatggcagg aggctctttc
     9721 ttttatttcc gaaaaattag aaaacatttt ttcagaatcg attggctctc catttattca
     9781 ataatggttt gtctaacgat attgttggga taattcttaa agaagtatta gaaatagttg
     9841 gtacttcatt actttacatg aagatttatt ctatggtagg agcaattata ttgagaatta
     9901 atcctttttt atataagttg tttgcttatt gat
//
LOCUS       DAMEET010000087         9874 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.088, whole genome shotgun sequence.
ACCESSION   DAMEET010000087 DAMEET010000000
VERSION     DAMEET010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.088"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(204..1052)
                     /locus_tag="PB780_002133"
     CDS             complement(204..1052)
                     /locus_tag="PB780_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDE0734173.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1051..2118
                     /gene="gnd"
                     /locus_tag="PB780_002134"
     CDS             1051..2118
                     /gene="gnd"
                     /locus_tag="PB780_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HDE0734174.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            2120..3121
                     /locus_tag="PB780_002135"
     CDS             2120..3121
                     /locus_tag="PB780_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HDE0734175.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3118..3402
                     /locus_tag="PB780_002136"
     CDS             3118..3402
                     /locus_tag="PB780_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HDE0734176.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3435..4232
                     /locus_tag="PB780_002137"
     CDS             3435..4232
                     /locus_tag="PB780_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDE0734177.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4246..5079
                     /locus_tag="PB780_002138"
     CDS             4246..5079
                     /locus_tag="PB780_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDE0734178.1"
                     /translation="MEILSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5097..5954
                     /locus_tag="PB780_002139"
     CDS             5097..5954
                     /locus_tag="PB780_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDE0734179.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            5976..6485
                     /locus_tag="PB780_002140"
     CDS             5976..6485
                     /locus_tag="PB780_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDE0734180.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6522..6923
                     /locus_tag="PB780_002141"
     CDS             6522..6923
                     /locus_tag="PB780_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734181.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7035..7388
                     /locus_tag="PB780_002142"
     CDS             7035..7388
                     /locus_tag="PB780_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734182.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7422..7865
                     /locus_tag="PB780_002143"
     CDS             7422..7865
                     /locus_tag="PB780_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDE0734183.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7882..8967
                     /gene="uxuA"
                     /locus_tag="PB780_002144"
     CDS             7882..8967
                     /gene="uxuA"
                     /locus_tag="PB780_002144"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDE0734184.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8960..9673
                     /locus_tag="PB780_002145"
     CDS             8960..9673
                     /locus_tag="PB780_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDE0734185.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         3280 a         1566 c         2181 g         2847 t
ORIGIN      
        1 actgtatact aactttgtta tcgcatctaa caagtaactc gaataatacc aatacaacct
       61 tctccaaaga tgcgatgaca tacgttctaa cccaccgact attgatgttc tgtaccaccc
      121 ttgcagaacc aaaaaagcca ctaccaaata tttggtagtg gctttttttg ttttatggat
      181 ttttcgcttt ttctgttatg gtgctattct ttgatttcag aaatagtatg tcgaacttta
      241 gcgatagaac gacgtgattc tttttcgttg taaaacatca aaatcacata tagagagtcc
      301 aaaatgctta gttgagaaat acgggaagct aaagcttcag atcggaattc tgtttcttca
      361 gaaatagaaa taaaaacgac atcgccaagt ttagcaaggg aagaattagc ttggctagta
      421 ataacgattg tttttgctcc agtttttctt acggtttcag caatatggat tgtttctttt
      481 gattgccctg tgtgagaaat caaaacagca cagtcttttt ccgtcaataa agaagcttcc
      541 atcaattgaa tatggtaatc cgtgctgtga gaagttgcga taggggaacg caaaaattta
      601 tgataagcgt cagttgctac aatctctgaa ccgccaacac cgaaaaaata gacacgttta
      661 gattcattga taatagtagc cgcttttttt agatcctttt gttgtaacaa cttttttgtg
      721 tcatttaaag ttgaaatact agactcgaac accttttgcg ccatagtgag ctcagtatct
      781 gttttctgaa tattttcgtg gatagaaata gcagatagat cattttcttg gatcaacatg
      841 gccattttaa aatctttaaa tccattataa ccaagttttt ttgtaaattg aaaaaaagta
      901 gagtcagcaa tgcccagttc acttgctaaa ttgctaatag gactatgact tactttgctt
      961 ggattttcta aaatataatc ggcgatagcc tgttcttttg ggctgaagtc gttgtacatt
     1021 gttttgattt gtagtcttat ggagacgctc atgagaattc ctcattttca taataagatt
     1081 atcatttttt ccgttctcat taatgttaaa gatacaagga gagtaggaaa aaatcaaaca
     1141 aaaaatattt ttgtaaaatc tatttacaaa aaaatttttt tgtataatga gggtataaga
     1201 aatggaggcg ctatcatgga aattggtgta attggtttgg gaaaaatggg attgaattta
     1261 gctttaaact taaaagagca ccatcacaaa gtacaaggac tcgacatttc aagtacagct
     1321 gttagtcagg caagagaaaa tgacattcct gcttatcaag agatctctga atttctagct
     1381 acttttgagc agaaaaaaat catctggtta atgcttcctg ctggagagat aaccgaaaac
     1441 atgttaagaa aactttttcc aaaactcgga ccaggagaca ttattattga aggcggaaac
     1501 tcttattata aagattcaat ccgtcgagca gaagagtttc agaaaaatga aatcggttac
     1561 ttggattgtg ggacttctgg aggaatcgaa ggagcacgac atcaagcctg cttaatgatt
     1621 ggaggagata aagaaacttt tctatccgtg gaacaattat tcaaagatgt agcaattgaa
     1681 agaggatatc tgtatgctgg tgcatccgga agcggacact tcttgaaaat gatccataat
     1741 ggaatcgaat atggaatgat gcaagcaatc ggtgaaggat tccaacttgt acaaaaaagt
     1801 gagtatgctt ttgacttgga acaagtcgca cgtgtttgga atcatggttc ggtgattcgc
     1861 ggctggctga tggaaatcat ggaagatcag ttaaacgaac atcccaattt aagtaattat
     1921 cggggaatcg tttctgcatc aggtgaagca aaatggacgg ttgaaacagc gctagaaatg
     1981 gctgttccag ttccagtaat tgcaactagt ttgttcatga ggaatctttc acaggaagaa
     2041 gatagttttt cagcaaaagt agtttcagca ctaagaaatg ggtttggcgg acatgaaata
     2101 gttgaaaagg gtgagtaaaa tgccagatta tcatatagca atcgttaatt ccagcagttt
     2161 cggaagagtc tttccacacc acttggaaag attaaagtca gttggagacg ttcatttctt
     2221 tactgttgat caagagattc caggaaaaga actagcggag ttacttaaag gattcaatat
     2281 cattattgca agtgttacgc cattttttcg acgagatttt tttgaaaaca aagatgaact
     2341 gctattgatt tcccgacatg gtattggtta caacaatatt gatttaaaag cagcaaaaga
     2401 acaccagaca atcgtatcta tcatacctgc tttagttgaa cgaaatgctg tagcagaaaa
     2461 taatataact aatttgttgt ccatgcttcg acgcacgaca caagcacaag caagagtcaa
     2521 ggacgatcga tgggaagatc gggcggagtt cgtcggtagg acgctgttta acaaaacagt
     2581 tggtgtgatt ggtgcaggaa ataccggaag cggagtaatc gaaattttgc gtaacggttt
     2641 tcgctgtgat gttctagctt atgatcctta caaatcagat ttatacatcg aaagctttgg
     2701 tgcaaaaaaa gtagaacttc aagaattact ggaacaatca gatatcattt gtttatgtgc
     2761 gaatttgaca gaagagaact atcacatgat ttctgataaa gaagtcgatc agatgaaaga
     2821 aaatgtttat ctatctaata gtgcaagagg cgctctagtt gatgaagaag cgatgattaa
     2881 tggtctgaaa agcggaaaga tcgcaggata tgccacagat gttttagaag aagaaccggg
     2941 acgtcaagat catccgtatt tagcgtttga acaagtagtg attacgccac atatatcggc
     3001 ctacacaagg gaatgtttag aagcaatggg tgaaaaatgt gtcagagatg tcgaacagat
     3061 cagtcagcac cttcttcctg aacgttctgt ccagacaaag agcaactata taaaaggatg
     3121 atgaagaaaa tggtgaacgg tttaaaagtg aaaacaggcc ctcaatttta tttatatgaa
     3181 gaaggtggta tttcaaaagt ttctgatctc ttgaaatcat acggcgcgaa aagagtactg
     3241 gtcacccacg gaacggtatc atgggaaaag gcgctaccta aattagtatt tttgaatgac
     3301 gaaacgattc aattttttta tcatcgttat tctggagaat gtagttatgc tgaagccaga
     3361 cgaatagcta caatcatcaa aaaaatgaaa tcgatttcgt agttggcgtt ggcggaggaa
     3421 aactgaccga tctagtgggt tatagtgcca atcttgcaag aatcgatttc ggagtcatcc
     3481 ctactttagc tagtaattgt gctccatgga cacctcttgc tgtcatgtat aaggagaatg
     3541 gacaagcaga agggaaaaca gaacactttt acagacaagc tgcttttttt ataacagatc
     3601 cccaattagt gattgattcc cctgttccat atttcattgc cgggttagca gatacactag
     3661 caaaatggta tgaatctgag ctcatcttag aacaagattt tttgcaagat gaatcatttt
     3721 tgaagctagc acaagatact gcgaagatct gcaaagaaga gattttgaat cattctgcta
     3781 aagcaatcca agatatggca caaagaaaac tgacatcgga gttcaaacag ctttcagaga
     3841 ttatctttgc tgttgctggt ttagttggag gattaggaga taaatacgca agaaatgcgg
     3901 cagcacatgc tatgcatgac gctatcagta aatatattcc ggaaagccat cgttttctac
     3961 atggagaaaa agtagcgtat gggattttct atcaactggc actagaaggt cgctgggaaa
     4021 aaatagacga gttatatcct ttgtatcggg aattagaact gccggtttct ctagaacaaa
     4081 tgggattatt tccgaaagat aaacagataa tagataaaat ggctgaattt attgattcga
     4141 aagagaaagt gcatttgatt cctatagagg taacacagaa atcactgata cagtcaatga
     4201 tggaactgga aggttatatc agaaagatct gaaggaggag gacacatgga aatattaagt
     4261 gtagtacaaa gtttgttgat tgctttatgg gtagccgcgg ttatgtcccg ctggttaggt
     4321 ggaggagcca cgttgacctt acgtttttct cctttgatga ctggattagt cgtcgggttg
     4381 atcatggggg aagttgcaca agcgatgatt gttacagccg ctttgcaaat gatttatatg
     4441 ggggtttttt ctccaggagg atcaatgcca gctgaaccat ctattgcagc agctatcgca
     4501 gtaccagtcg cattattagg ggatctgtcg ccagaagcag cgattgccgt tgcggtgcca
     4561 gtcggattgc tcggaagtta cttgtatcag tttcgttttt tcatcaatac ttttttaggt
     4621 aaatatacag acaaagctgt agaagatttg aatacaaaag ctatggcccg atcaattatt
     4681 ctttatccaa cggttgcctc atttttatta ttcgttccgc ttgttttcat tgctttatat
     4741 ttaggggcgc cattgatcgc tgatgtaatc aaagctttag agggaacagt cgttatccac
     4801 attcttgaag tagtaggtgg agggttagcg gcaatcggta tcgcgacaac tgtatacgtt
     4861 atcggtcgaa aagattatct ggtctttttc ttccttgctt attttatgag tgttgttttg
     4921 aaatctttag atattacgat ggttacttat gccatttttg ggattattat cgctttgatc
     4981 tttgtccagt cgcaaaaagg gaaacaagta cagcctgcca caagcaataa caattcagca
     5041 tcttttgatg acgaagatga ctatgatgac ggattttaat ttaggaggaa tgggaaatgg
     5101 ctgaaataat ggataatcaa acaggtgctc ctgaagaaat cacgaaaaaa gatgtaacaa
     5161 aagcctatct gagatggcat tttgcaaacg aaattcctca ctcttttgaa cgttacttgg
     5221 cgccatcact actatacgga atgatgccaa tcttgaaaaa actttataaa gatgaagact
     5281 ctttaaaagc agcatataaa cggcagttac tgttcttcaa cactcaatta agctggggtg
     5341 gcggtgtgat tactggccta atgtcttcta tggaacagca aagagcagaa gaagagtatg
     5401 aagaaaaaga gatcttgatg caggatgact tgatgtataa cacgaaagcg ggattgatgg
     5461 gggcactagc aggaatcgga gatgcaatcg attcaggaac cgtccaatat atttttatcg
     5521 caatcgcggt accttgggca caacagggaa gtcctttagg cgcacttttt ccttttatct
     5581 gctttgggtt gtatcaggtt ttactagggg tatttttcgc aagacaagct ttcactatgg
     5641 gccgtaatgc tacaggccta atgcaaaaca ctggtgttca gacagcgatt gaaatgctat
     5701 cagttttagg gttgtttatg atgggggtgc ttgcgggaaa ctatgtaaaa gtagaatctt
     5761 cattgaaatt tgcgatatcc ggacgtgagt ttgttatcca ggaaattctt gatcaaatcg
     5821 tgccagggat attaccttta gctgtcgtaa tgggtgtgta cttgttctat acgaaaaaag
     5881 gattgaaagt cacacaagca ttattatggc tgactggaat attaattgtg ctagcaggaa
     5941 tcggtatttt ataaaaagaa gggaacgtga ataagatggg tgtagtcaat ttagcgcgtg
     6001 tggatgagcg tttgatccat ggacaagtaa tggtaacgtt atctcaaaaa agtggagtga
     6061 attccatttt tgttgtagac gaagttgtag caaaagataa atttatgaga gatctttaca
     6121 aaaatgcagg aagccgtaca ggacaaaaaa caattgtcat tacaccagag aaagctaaat
     6181 tctattggga tgaatatcaa tttaaagaat acaattgtat tttgatcgca aaaacagtcc
     6241 aaacgattta cgacttagta aaacacggaa tccccatgga agaattgaat attggaggga
     6301 ttgctcaaaa aaatcctgat acagatcttt tagtgacgaa atctgtttat ttaaataaat
     6361 cagatgctga aaaattgaaa gaaatgaatg aacaatatgg cgtaaagaat atttattttc
     6421 aagccactcc atctgcacct aaaacaggat taaaagatgt attgaacaag tttgatttgt
     6481 cataaacaaa tgcttagaaa aaagataaag agggcgaaaa aatgaagaag aacgagctgt
     6541 ttcgtgattg ggaatttcgt tatcgctata tttaccgtaa gcgccgtaca aaaaagagta
     6601 aacagcgttt tctttcagca ttagtatcag atatttacag tatgagaaca gatgtgacag
     6661 tcattgctta cgatacgcta gcctaccgat caaaaaatat ttatgttgga gatatcgaaa
     6721 aagcagagaa agttatctgt acttattacg atacacccgt tcatgcatta ggaagttatt
     6781 tcatgtttga ttggaaagac caaaggaaaa aaacaatcta ctctatatta ttgtccttca
     6841 ttttgttgtt cagtttaggt tggtggggaa tgatgatata taacaaaaat cctcatcacg
     6901 tattcgatct gctatccgta taaacaggta tcactgtact agcatttggg agctactttt
     6961 tcttattggg aaaagcagct cgcggatggc ctagcagaca aacttttatt cgaaatactt
     7021 catccatatt aacgatgcta gaaatgatcc gtacgattga tgatccgagt gtagcgtatg
     7081 cgtttgttga tgaaggctgt tatggagaaa agggcttaga cagcgtgaga tcgggtatga
     7141 aaaaagaggc tattcttttt tatctagact ctgttggggc agatacaccg ctacagtttt
     7201 cgggaaatta tttttcaaat aaagaacagt ggctgaaaca agttgataag ttgaaagaaa
     7261 aaaacgtcaa ttatattttt agtgcaagaa aaaagcaagc gcagttcttc tatttgacca
     7321 aaacagattt acgagggaaa acatttaatt ggcaaaatgc caatcagatt attgctcttt
     7381 ttcgataagt aaaaagtgca aaacaaggag tgagaagact aatgttggaa atcatgatcg
     7441 ttacacatgg taagttgagc gaaggactga aagatgcggc ggaagtaatt gtcgggacat
     7501 cgagccatat tgaaacaatg tgcttagagt ctggggagga catccaaaca ttcggagaga
     7561 aggtcaaaga aaaattaact atgatcaaca cagcagaacc catgctagta tttgttgatc
     7621 taattagcgc aagtccgtac aatcaagcgc tcttggcagt caatgaactg actgaagaaa
     7681 agaaagcagc cacttatgtc atagggggcg tgaatctgcc aatgttgtta gaagcaatca
     7741 atcatcagat gcttcacaca ccgatagaag aagcggtgga acaaattgtc aaacaaggaa
     7801 aagataattt aactgtttgg catgcttcag cggaagaaaa aaaattagat gaggacgatt
     7861 tttagaagga aggctgaaaa gatgaagtgg ggatttagat ggtatggtga aaaaggggat
     7921 tcgatcccat taaaatatgt tcgccaaata ccgggaataa atgggataat tggaactttg
     7981 ctgactaaaa tgcctggaga tgtttgggag atttcagaaa ttgaggagtt gaaagcatct
     8041 gttgagagcc aaggactgga actgcttgga attgaaagtg tagcgatcca cgatgcaatc
     8101 aaggcaggga cagatgaacg agattactat attgataact atatacgaac aatcaaaaac
     8161 ttgtctcgat gtggggttcg attgatttgt tatagcttca aaccaatttt tggatgggcg
     8221 aaaactgatt tgtttaatga aaacaacgat ggaagctatt ctcttttata cgatcagaaa
     8281 atcgtcgatc agatggagcc aggagaaatg tatcaattga tacacagcca gtcaaaggga
     8341 ttcaaattgc cgggttggga agaagaacgg ttgaaaaaat tcgctcagct ggaaaaactg
     8401 tatgaaggaa tgaccgaaga agaattattt gaaaatctga cttatttttt agaaaaagtg
     8461 attccagtgt gcgaaaaagc agatgtaaaa atggctatcc atccagatga tccgccatgg
     8521 gaactttttg gttttcctag agtgacgaaa aatcttggcg acttgaagaa aattctaaat
     8581 gcagtagatt ctccatataa tggactaacg ctgtgcacag gatctttagg ggcaaatccg
     8641 gaaaacgatt tagttgaaat catccatgag gtaggttcgc gcatccattt tgtccacttc
     8701 agaaacgtgg agtttttagg agaaagaaag ttcaaagaaa cggcacatcc ctctacagaa
     8761 ggctcccttg atatgtatca tattatgaaa gcgttggtgc aagaagggtt cgacggtcct
     8821 atacgccctg atcatggtag aaccgtgtgg ggtgaagtgg ctatgcctgg atatggcctg
     8881 tatgatcgtg caatgggact cacttacatg caaggtctat atgaggcgat aagtaaggaa
     8941 aaaccactgg gagagcatta tgaataaaat caagctcttc atctttgata tggatggcct
     9001 tttattagaa actggacgtt tagcttatcg tgcttatgta aaatcagcac aaaagtatga
     9061 ttatgaaatg cgtaaggaag tctattattt actaactggc caaacagaaa tggcgataag
     9121 agaacaaatg ggcattttgt acggagaaga tgttccatat atcaaatggc gagaagcaat
     9181 caatcaatat aaagaaaaaa tcgtgaaaga agataagaga gtatatacga aaaaaggagc
     9241 agaagaaatt ttatcttttg caaaagaaag aggaatccat acaatcgtgg cttcgtctaa
     9301 tacacgggta aaagtagaaa tgtatttaaa aatggaaaat ctatatgcct tgtttgatca
     9361 aatcatttca ggggatgatg taaaaaaagg aaagccggaa ccagagatct ttttgaaagc
     9421 ttgttcaaaa atgaatattc ctccaagtga agcacttgta tttgaagact cgattgcagg
     9481 gatcgaagca gcaaggcgag cgggcatttt gtcttttctc atcaaagatc atttgatagg
     9541 gttgcccgat cataatggga agcataagtt gaaagtagat gtaagtttat atgaatccaa
     9601 taaagcagac tatgaatttt atgacctgca tcaagcaaaa cgatttttag aaagtaaagg
     9661 gttgtatctc taataaagag gactattttc tagtcctctt tatttattgt cctgcacgac
     9721 tgagggaaca gatgaaaaag aagtaatttt ttattttgaa gtatctacct tatttgtagt
     9781 gaacaattta gaacaaacac aaaacgcatc tactgttatc taccagtaga tgcgtttaaa
     9841 gagaagaacg agaatcgtcc atatgagggt ttcc
//
LOCUS       DAMEET010000088         9633 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.089, whole genome shotgun sequence.
ACCESSION   DAMEET010000088 DAMEET010000000
VERSION     DAMEET010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.089"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            50..448
                     /locus_tag="PB780_002146"
     CDS             50..448
                     /locus_tag="PB780_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDE0734186.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWVMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            727..1227
                     /locus_tag="PB780_002147"
     CDS             727..1227
                     /locus_tag="PB780_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HDE0734187.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRVIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(1310..1861)
                     /gene="lepB"
                     /locus_tag="PB780_002148"
     CDS             complement(1310..1861)
                     /gene="lepB"
                     /locus_tag="PB780_002148"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDE0734188.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(2013..2240)
                     /locus_tag="PB780_002149"
     CDS             complement(2013..2240)
                     /locus_tag="PB780_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HDE0734189.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(2237..2755)
                     /gene="msrA"
                     /locus_tag="PB780_002150"
     CDS             complement(2237..2755)
                     /gene="msrA"
                     /locus_tag="PB780_002150"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDE0734190.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(2775..3488)
                     /locus_tag="PB780_002151"
     CDS             complement(2775..3488)
                     /locus_tag="PB780_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HDE0734191.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(3491..4387)
                     /locus_tag="PB780_002152"
     CDS             complement(3491..4387)
                     /locus_tag="PB780_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDE0734192.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(4545..5387)
                     /locus_tag="PB780_002153"
     CDS             complement(4545..5387)
                     /locus_tag="PB780_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDE0734193.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEEILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            5519..6172
                     /locus_tag="PB780_002154"
     CDS             5519..6172
                     /locus_tag="PB780_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDE0734194.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(6236..6763)
                     /locus_tag="PB780_002155"
     CDS             complement(6236..6763)
                     /locus_tag="PB780_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDE0734195.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEIDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6783..7730)
                     /locus_tag="PB780_002156"
     CDS             complement(6783..7730)
                     /locus_tag="PB780_002156"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDE0734196.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKEVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(7742..9628)
                     /locus_tag="PB780_002157"
     CDS             complement(7742..9628)
                     /locus_tag="PB780_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDE0734197.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSGDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
BASE COUNT         2601 a         1902 c         1690 g         3440 t
ORIGIN      
        1 tcctaaataa gagatgatag acgaacaaaa cagaaaggag acgaaacaaa tgaatagtat
       61 ggtttttgtt aattttcctg ttgctgatgt caatcgttcc gttgcttttt atgaaaagct
      121 gggttttaaa aaaaatgaag aattttctac tgaagaagct ggttcaatgg tatgggatga
      181 cactttttgg gtaatgcttc tctctcatga tttttataaa aaatttataa aagataaaga
      241 aatagctgat aataaaaaaa caagtggtgc tttgatttcg tttagtgttg aaaacgcgga
      301 tgaagtaaaa aaatttgctc aaattgcaaa agaaaatggt ggtactttcc acgaagtaga
      361 tatgggaatg cctgaagaag aaatgttttc attagaagtc caagatctag acggaaatac
      421 attagaaccc gtatggatga agatgtaagc agataactaa tctgacgttt tttataacaa
      481 aaaaacaaag gcggatgata cgataagtat tctccgcctc tatttttttc attcgtacaa
      541 tctctttttt acagtttata aaagcagtct tctcaccctt tttttaattg ttagatgcca
      601 gtgtatgtaa atcaccaatg tgtcatgaca cattggtgat tttatggtat ccttttatta
      661 ttaactgaca aaggagagaa tattttgaac actttgattg caaaagacgt ttttagattg
      721 cccgaaatga atgcaagatt gttgtcgacc tctgctcttt tattagccct tgatgtcatc
      781 ttatacaaga tagcgattgg tcctgcttat tttcaaatca cattcggatt tttaagtatg
      841 gcgcttatgg gttatctata tggaccgatc tgggctggtt tgcttgaaat gttgtcgaac
      901 actttaaatt tcacgatatt tggttcagga acgtttcaag ctgcttttct tatcacggct
      961 ttccttggtg gatttattaa tggcttattt ttatatcata aaaagataaa cctgccttcc
     1021 gtcatcgctg cccaattatt gattatgatc ccagtcagcc tttttatcaa ttcttggctg
     1081 atgtctgttt tgtttggtgc agatttcaaa gtgatttttc taggtagagt catcaggaat
     1141 atgatccaaa tccctattca aataatggtt gtttattcat ttatcaaaac tttagaggct
     1201 agaaaagtgt ttaaacgagc tgtttgattt tacgtactaa ggtagtaaac tgattttgga
     1261 ttaaacaata aagcgtgcag tctcaacaga ttgcacgctt tattgtttct cactggatcc
     1321 attttatttc atcaagtgga taatagacaa atcttgcttt acctagaata tcagttttat
     1381 tgatcgtgcc aaacgaacga ctatcttttg aaactcttcg attatctcca agtacaaaat
     1441 aattatcttc cggaagcttg gattgtcccg ttagctcttg tagtgtaaag tccgtcgtat
     1501 aggatgcatt tgcatgatct gatttcaggt ttttagttag ataaggttcc ttaactactt
     1561 tcccattgat cttcaattga tcattttggt agctgacatt ttctcctgga agaccgatta
     1621 ctcgtttgat ataaatcgtg ccatctgcta attgaaaaac aacaatatca aatcgtctga
     1681 tctccgaaaa tttttccatc acaaccatat caccttgctt caacacgttt tccattgaat
     1741 tgccttccac tggtaccggg atgaataaaa atccccgcaa catgaacgca aggaatacag
     1801 aaacaagtaa gtatttgaat acaagccaga agcgattaat atagcgttgt ttttttgtca
     1861 atctctatca atccatccct cgttttttca ttctttcttt attataagaa aaatatcaaa
     1921 aaaatgcgac tagatagctg aaataaaaat aattctttca attaggctat tggacaaaaa
     1981 acttttttga tcataaagta caaaactatc tcttatatgt catgattatt ttctaaatat
     2041 ttttcccatg cttcgtcaaa gatatccatg gacggcaaat agtcgacatt cagttccaga
     2101 taattagaaa tttcttcgta gctagagctg tgcttaggaa attggatgtc tttaccggct
     2161 tctgtagcga aaacagataa agcatcatgt gtgccaccgc ctcgcagtgt cattaaataa
     2221 tgaaaaaagc ttcgattcac ccttcgttcc tccaattttc ttcgataaag gcagcccggc
     2281 gtgcatggtt tcttttaaaa ttttcaggat gctttttata atactcttga tggtcttctt
     2341 ctgctagata aaatggttgg gctggttcga tttttgtaac gattggctgt tcgaaacgtc
     2401 cactttcagc taactgtttc ttactttttt cagcaatgat ccgctgttct tctgtcaaat
     2461 aaaagatcac cgggcgataa ttgtcaccgc gatcctcgaa ttgtccgaaa gcgtcagttg
     2521 gatcagtttg ctgccagtaa agttctacta attgttcata tgaaatcaat gttggatcat
     2581 agataatctc cactgcttct gtatgtccag tcgtttggct ttttacttgt tcataagtcg
     2641 gatctggtac atgccctcca gtatatcctg acatcactgt atggatacca ggaagcgtgt
     2701 cgaaaggctg gatcatacac cagaaacagc ctcctgcaaa tattgctcgt tccattatga
     2761 ttccccctct aatactactc atccgatgat tttttatttt cttttgtctt gctttcagta
     2821 ctgctggtgc tagttttgtt ggtatttgag ttgtttgaag catcagaggt atcttttggc
     2881 aaatatagac taaaccgaat gtcatcattt actaagtcga ttttatctgc tttaatatac
     2941 atcccgtttt gcattcggaa ttgatccaac ctaagcatga tagtcagatc atctgtattg
     3001 acttcgatcc aatctggtaa tttatagttt ctcttgatca tattcatcac atctttgatt
     3061 ggcaaactca atgttccgat agaaagactt ctagctttta gttggacatt tccgttttcc
     3121 atcacatacg gatcaaaata taaatagaaa tcgataggaa aacctagtac ttcgaaagta
     3181 ccattgagta gtgcttcatt ttccaaataa aatttatatt caatatcact atctttttga
     3241 tactcactaa ggaaaaagct gatgatttga ttgacttttt ctttatttga attgatcgtt
     3301 aagacaggat ctccttgtct ttccgtaaga gctggtattt ctgggagttc tggttcccga
     3361 ttagcaaaaa tacggctgcc taaaaatacg gtcgaaccaa gtactacccc aagaaggata
     3421 aggaaggcga atttccagta atttgttttc tttgttttgt ttgaagatac gtcagatctt
     3481 gttttcatgt ttattgatca tcctccttct ctagccattg gttttgtgtc tcgatcagct
     3541 gatctctcac tgcatttgcc atcaattgat aacctaaatt atttggatgg aaatgatctt
     3601 cttcatagag cacattattt ttgatgccat ttagattttc tgtcgtgcta gttgtttgat
     3661 ctgttccttc ttctgtgaca cctacttcct gattcaatcc ttgataaagc aaatcattga
     3721 ttgggataaa gtagcagttc ggcatttttt ttgtcaccgt ttctgttcct ttgttccaat
     3781 tgttaacaat tgtctgcatg tcagtgatct caggaaagtt caaataaaat ggattgtaga
     3841 tgcctaaaat gtaaataggt gcggttttat tgagggatcg aaattcttcc aatagttctg
     3901 tgacattttc ctgatagttt ttcagtggct ttttaaagga attgaccgtt aacccgaata
     3961 aattattttg gataactttc atcaaatcat taccgccaac agtcagtgtg ataaaatcag
     4021 ctgttttgat attgtttcga atttcttctt ttgtttttac tcgctttaag atctggtcac
     4081 ttcgttcacc atttacacca tagttttctg tttcaaccgc ggtcaaatta taacggtctt
     4141 ttagatcatt tgcgacaagg ggaacgaatc ctccttgctt ggtttgatct cctactcctt
     4201 cagtcagcga atctccgata gctatgtagt gaagtttttc ttttttattt ggatcttctc
     4261 tcacagtagt tactggtttc attagtgggc cagctttcgg tatagaagca gctaaacctg
     4321 catagatgat gatcgcaatt aataatggga acaaaacgag gatcagatgt cgtttcgtta
     4381 atttcatttt ttcacctctt gtattcgatt ggttctaatg ttatcaaaaa ttttatcaaa
     4441 aaggtagtct ttatgaaaaa agaaaacaat tattttccta attttataaa gaaaaagaga
     4501 ataagctgca agcctttcag gctgagggct tatcctcttt tcgactaatc tacataatac
     4561 atgattgcaa acgcgtcttt accagtatgc gtagcgataa ccggattcgt atgcagtaca
     4621 gggatatgca tgtccgggaa taattcttgc aaaccctctt tgaattcttg cgccaattct
     4681 tttgcatccg catgtgaaat accgatttgt cgcacatttg gcaattcttt cagttcattt
     4741 ttcaaattat taaaccattt atcgaacgtt ttctttcctc gccccttagt tacagggatt
     4801 aattcagaat gggcgaaatt catcaccacg cgtatgttca aaacactaga cagcaaacca
     4861 gttgttcgac tgattcttcc accttttacc aagttatcta gtgtggatat tccgatgtat
     4921 aattttgtat tgtcttgtac ttttttgact tcttcaagga tttcctctac tgttttacct
     4981 tcttttgcta attcagctgc tcgaattacc tgaaaagata atccttggtc agtggtatca
     5041 ctgtcgataa ccgttacttt cgtttttgtt agattactgg cttgtcttgc tgcttctact
     5101 gttccactca agccttttgt catatggatc gaaatcactt cactgccgtc ttctcctagc
     5161 tcatcataca tcgagataaa ttctccaatc ggtggttggc ttgttttggg cagatttttt
     5221 gcttttgcca tcatttccat aaatctttcg ccgtctagtt ggtcatcatc gggatatact
     5281 gttccgtcga tcatgatgga tagcggcatg acacgaatgc ctaaagcatc gcgtacattt
     5341 ttttccatcg tacatgaaga atctgttacg atttttacat ttgtcattat tttctctctc
     5401 tctttcttta acaagatgcc ttattcatgt taaactacat gtaagacaat ttctattttt
     5461 agtataacag acaaataatg atttttcatt aattacacct agtaaaggag ccgttttcat
     5521 ggaaaaaacg aaatttaatc gaacgtatga aatattaaat gaagtgttta atgcagtcac
     5581 ccatggaatt gggactggat tgagtatcgc gggactagtg gttcttttag taaaaggtgc
     5641 ccacttgcat tcctccctcc atattgtctc ttatgctatc tatggatcga tgatgattct
     5701 tttattttta acgtctactt tgtttcattc attgattttt acaaaagcaa aaaaggtgtt
     5761 ccaagtattc gatcatagtt cgatttttct tttgattgca ggaagttata ctcctttttg
     5821 tttgctgagt atccgaggat ggctaggttg gactttattt gtattaatct ggttgtttgc
     5881 gattcttggc attatatata aagcgttgac gttgcataaa cgggagagag tatcaaaagt
     5941 atctacgatc atctatattt ttatggggtg gctctgtgtc gtggctgctg ttcctcttta
     6001 tcactctctt ggaatgactg gtgttatttt gatggtagca ggcgggcttt cttacacgat
     6061 aggcgcattc ttttatagct tgaattctat ccgctatatg catgttgttt ggcatttgtt
     6121 tgttatgtta ggtgcaggtt ttatgtactt ctctgtttta ttctttactt aataaaaaaa
     6181 aataaagcta tggatgggac agctctaaat aacagctagt ctatccatag cttttttata
     6241 tcgtcaattc gcctgtcccg ctttttacag gattctcatt acgctgatac gtttcaaatt
     6301 gatgcggata acgatttttt tcatctattt ctccttgact agtattgatc agtgaccaat
     6361 cagaccagtc aacaggtgga aaataagcat ctccatcgaa tacatgatgg atgactgtac
     6421 ggtataatat cgtacaaaat ggcagaaatt cttgataaac agctgaccct ccagcgatga
     6481 aaaagatacc gtctgtttct ttttccagag caagcacgtc ttccacacta tgcatgacat
     6541 gcacgccttc tgctttatag gaagaatctc tcgtcaaaac aatcgtctga cgattcggca
     6601 acggacggct acccatccct tcgaaagttt ttcgtcccat cactagtgta tttgcttctg
     6661 tcatttgttt gaagaatttc agatcatttg gcaaacgcca aggaagacgg tcttcttttc
     6721 caatcaatcc gttttcatcc tgtgcccaaa ttgcagctaa cattttctca tcctttctga
     6781 ttctaaacag cgataggagc tttgatcgcc ggatgtgggt catatccttc taattggata
     6841 tcttccatct caaaatcaaa tactgactgc ttttcctgat tcaaaactaa agtcggaaat
     6901 gcgcgtattt ctcggctcaa ttgttctttc atttgttcga tatgattgat ataaagatga
     6961 gcgtctccta atgtgtgaac aaattcgcca accgctaaac ctgtttcatg tgcaattagg
     7021 tgagtcagca acgcataact tgcaatgttg aacggaacgc ctagaaacac atccccactg
     7081 cgttgataca attggcagct tagccgtccc tcttgaacat aaaattgaaa catcgtatga
     7141 caaggaggca aagccatgct aggaacatct tccggattcc atgcagaaac gatcaatctt
     7201 ctagaatcag gagtcttttt gattgcttcg atcacttctt tgatttgatc gataaagcca
     7261 ccgtcttttg tttcccagtg acgccattga tagccgtaga tgtgtccaag atcgccgtat
     7321 gtttcagcaa aggaatcatc ggttaggatc ttctcgcaaa aaagcttgct ctgcttttta
     7381 tattcttcat tgaattcagg atctgtcaaa catctatggc caaaatcagt catatctggt
     7441 ccattatagt ctggactttt gatgtagcgt tcaaatgccc attcatccca aatatggttt
     7501 ttattttgta atagatagcg gatattcgta tctcctttta aaaaccaaag caattcgctt
     7561 ttgatcaatc caaaaggtac tcgtttcgtt gttaacaaag gaaacccttt ttgcagatca
     7621 aaccgcattt ggtatccgaa taagcttaat gtgcctgtgc ctgttctgtc ttctttacga
     7681 tgcccttctt ccaacaattt tcttcctaag tcaagatacg cttgttccat cctttttcct
     7741 cctaatcttc tacccattca ctcaagtatt cccaccgtgc cattttttct tcaagttgtg
     7801 cttctgtttc agatacgtcg ttttgcaatt cttgtaatcg agtgaaatta tctccttgat
     7861 gattcatctc ttcttggagc aatgagattt tttcttcaag ttctgcaatc tcatcttcaa
     7921 tcgtttccca ttctttttgt tccatgtaag ataatttctt cttttcagca ggttccttgc
     7981 tctgtatttt tggtttcgct ttttcagcag gctgctgggt ctctttttgc ttcagtaaat
     8041 aactggacat gtttccgtaa taaagttctt gttttccgtt tccttggaat actaataact
     8101 tatctgctac cttgtccaag aaataacggt catgggaaac agtgatcaca gcacctggaa
     8161 aacttctaaa ataatcttcc aaaatcgtca atgttgctat gtccaaatca ttggtcggtt
     8221 catccaataa taaaacatta ggttgctgga tcagcagttt caacaaaaat aaccggcgtt
     8281 tttctccgcc tgaaagtttg cctatcagtg tcccatgcat aaatcttgga aataaaaatc
     8341 gttcaagtaa ttctgctact ccgatttgac tgccatctgc tgtttttgct tcttcagcag
     8401 cttcttgtaa gtaagcaatc atccgtttat ctggtgccat ttcttcattt tcttgtgtat
     8461 aatacgcaag atgaaccgtt tcaccgatag acattgtacc gctatccagg ggaattctgc
     8521 cagctagaat attaagcaat gtcgattttc ccgcaccatt tttaccagta atacctagtc
     8581 tttcccgaga ttgaatcagt aaatctaaat gttctagcag tgtttggtca tttatacggt
     8641 aactgccatc tttgatttcc agtacttttt tccctaaacg ttgtgtcgct aggttcagct
     8701 ctaaatcatc ttcttggttt acttggtaca agttctcctt tagatcttcg aaacggttga
     8761 tccgtgcttg ctgcttggtg ccacgtgcct gtacgccagc cctcatccaa gctaattcct
     8821 gtttataaag ccgcttgcgc ttttcttctt gttctacttc tacacgatca cgttctgctt
     8881 tttctaagac gtaagcttca taatttcctt tatactcgta taattttcca aatgaaagtt
     8941 cgaagattcg gtttgctact ctatctagaa aataacgatc gtgggtaacc atcaatagtg
     9001 cgccacggta ttgtttcaaa tagttttcca accactcgat cgcttcataa tctaaatggt
     9061 ttgttggttc gtctagtaat agtaaatcgg gttcatcaat caacacttgc gccagactaa
     9121 ctcgtttttt ctgaccacct gataaagtac tgattttttt atctaattca ctgattccta
     9181 atttttgcaa aataatcttt gcgttggtat ctgtagtcca agcatcttct ttattcattt
     9241 gttcttctgc tttagcataa cgtttttgca catcagggtc ttctccgttt tcccctagtt
     9301 cgatcaatgc ttcctcatat gctcgaacag tttggatcaa ggggctgttt ccttgaaaga
     9361 ctgcttggag aacagttaat tcatcggaaa attcagctgc ttgagaaaga taaccaatac
     9421 ggtaatcagt tggataaaac actgtttgcc gatcaccgtc gccgctatcg ataccggcaa
     9481 taatatttaa gagacttgtt ttacccgtcc cgttcgtccc aatcaacccg atacggtctt
     9541 tttcgtggac caaaaaagaa atctggtcaa acaaatcttt ttcaccataa gtttttttga
     9601 ggtcagttac ttttaattct ttcaacatgt tcc
//
LOCUS       DAMEET010000089         9446 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.090, whole genome shotgun sequence.
ACCESSION   DAMEET010000089 DAMEET010000000
VERSION     DAMEET010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.090"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(142..1086)
                     /locus_tag="PB780_002158"
     CDS             complement(142..1086)
                     /locus_tag="PB780_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDE0734198.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDMD
                     KQVTFIEGDVSDSQLMERIMKEYQFAYIFHLAAVASVADSVERPVETHRVNFDSALLL
                     LELARKYQSNLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKAEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILSGMERYLKTEI"
     gene            complement(1220..2611)
                     /locus_tag="PB780_002159"
     CDS             complement(1220..2611)
                     /locus_tag="PB780_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDE0734199.1"
                     /translation="MLNERFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFVINIFIQFS
                     FIALSGSRTTLVCLLITTFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVT
                     DITAHAWAHVPHITEVKKDTSDSSGTTSSNKVDLKRPDTDSGDVSNLRFELWKSAIEI
                     FKSKPISGGSPGYYIEYAHDKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLF
                     IQCFKALRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLGS
                     IQKTISKEEGVTN"
     gene            2737..3147
                     /gene="tagD"
                     /locus_tag="PB780_002160"
     CDS             2737..3147
                     /gene="tagD"
                     /locus_tag="PB780_002160"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HDE0734200.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGHFDE
                     LEQLTDAQVIYLPRTPEISTTQIKNELNSLNSDK"
     gene            3323..3736
                     /locus_tag="PB780_002161"
     CDS             3323..3736
                     /locus_tag="PB780_002161"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDE0734201.1"
                     /translation="MISISHSIKELFTIKDKNIEINSFQETFYKDKKAIVIEGPLVRT
                     FRHCLFCKESAKQIEKNGKKISMIILNRSGNKQTYLRLRKQHYHCQSCGKYFTAHTYL
                     VAPSCFISKQVHYTILEELTERQALKTIAKRCDFQ"
     gene            3893..4150
                     /locus_tag="PB780_002162"
     CDS             3893..4150
                     /locus_tag="PB780_002162"
                     /inference="COORDINATES: protein motif:HMM:NF013753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDE0734202.1"
                     /translation="MTFCPAGEKKDLIAYFMRFTRKAHMGVRYIVTDMNAPYFSLVKE
                     PFPNAQIIIDCFHIGQHLNKFFDRVRKRVMKQLNQKCQRHL"
     gene            4237..5475
                     /gene="istA"
                     /locus_tag="PB780_002163"
     CDS             4237..5475
                     /gene="istA"
                     /locus_tag="PB780_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HDE0734203.1"
                     /translation="MRKDIREGVMIYVINEIKPNYAALAKQYDCDYRTVKHAYEEAQV
                     KESKPPERKKRPSKLDPYREIIQDKIKDQCSAYSIFKFIEHKGFGGSYSLVKTYCRQI
                     KQAKVHKATVRVEHSPGLSAQVDWKEDMRLISNQGEIFEFNIFLYVLPYSKKKYVTLT
                     FDRKQDTLFECLDDAFYQTGGVPKEIWFDNMKTVVDHSRSQFSKPHFNKRFYAFSKDA
                     GFTPIACRPFRPQTKGCVEALARTVDRLRVYNHEFYDAVELCHLVNNLCVELNNEVSQ
                     STDQTPDERWQTNEKEHLHPYQKNLLNPYFEDFIRRKVTKEEMVIFRKCRYSVAPRYI
                     GKEVEIELSDKEDQVQIYYNGELIRSHPLTTKQLNYHEEDYFQILKSDVMSHKEDDGI
                     RAYTRESLKQYDLVEVADEE"
     gene            5465..6205
                     /gene="istB"
                     /locus_tag="PB780_002164"
     CDS             5465..6205
                     /gene="istB"
                     /locus_tag="PB780_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HDE0734204.1"
                     /translation="MKNSHYQELMTNLETLGLNHMREYVPNYIEVANREELSLTEALL
                     ELTRREVDHQNKQKMNRVVARARFSKRTSLEEFDFAFQPSINKREVLDLRHMGFVEKH
                     ENLVFIGSPGVGKTHLTIALGIEACRQGYRTLFITCHELLLRLRTAYEKGTLERVLKR
                     YARYDVLIIDEIGYLPIQKQEADLFFQLLAMRYEHRSTIITTNIVLSRWGELFQSSEI
                     AAAILDRLVHHVKVFKITGKSYRMRDKI"
     gene            6615..6779
                     /locus_tag="PB780_002165"
                     /pseudo
     CDS             6615..6779
                     /locus_tag="PB780_002165"
                     /inference="COORDINATES: protein motif:HMM:NF013753.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6846..8273)
                     /locus_tag="PB780_002166"
     CDS             complement(6846..8273)
                     /locus_tag="PB780_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HDE0734205.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFLQFLIGLSVSFLYIAY
                     IYFFDVKYKNLYLIQLIFIAGLTLDISWFFQGIEEFSKVVIRNTVIKLSSVLLIILFV
                     STKDDLWIYVFITSITNFLGSLVFWLSIHGKVGKPLFNSNVFKQLWKPAFIILTPQLF
                     MQVYTTLDKTIVGYFVDPTALSYYDQSQKIARIILAVLTSITIVMLPKISKAQAEGKS
                     ENILVYTKKSFDYTIIIALILFSVVFINTKEFVPWFFGENFKPMTINMLISCLIIVVS
                     PIGGVFANQFAIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATIVLVVVELLV
                     FFFRVFFVRKEIKISYFFGKNVVLFFIVNLTLTTSAYYFLPQLFSSSFLDMAIKSIMI
                     FIIYLPFVYFLFPDIRNEVRKIIRK"
     gene            complement(8270..9412)
                     /locus_tag="PB780_002167"
     CDS             complement(8270..9412)
                     /locus_tag="PB780_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDE0734206.1"
                     /translation="MKNIKKFFSRNIFPVFIKILPIQKNLIVFSSFGGRNYSGNPRVI
                     SEKIHEKKLNYRIIWLIKKEAIITDLPDYIKRIDSDSFLAAFYLYTAKYWIDDSRKTI
                     KYAKRKKQFYFQTWHGTPLKKIEFDAKDKLPKRYLSYAKKDSESITYLLSGNRYSSEK
                     YVSAFNIVPSKILEVGTPRNDELASSKEQVFDLKKKQINVLFAPTFRNNIEDNGITQL
                     EWLGVDNLREFFKKQQQELNLMTKFHPNVHSKLATDSKSIEYMKKHQITLINDGIPLE
                     MLFEEIDVLITDYSSIFFDFALTKKPIILFNYDEKEYSLERGFYIQLSELPIISVQDS
                     YGLVDVFKNQQEKLLKSSQELLDYIGNYETGASTEKVLELLERDIK"
BASE COUNT         3294 a         1701 c         1468 g         2983 t
ORIGIN      
        1 aagttctctt tttcatgtaa aatatttaat tatatatttc ctaaataaaa agtaaaacaa
       61 gaattaaata gttggttata caactttaat tcttgtttta ctttttagaa ttatttatcc
      121 aaagatgcaa tcattttttc atcaaatttc tgtctttaaa taccgttcca ttccgctcaa
      181 tatatcaaat ttaggctgat agcctaaaga acgaattttt gaaatatccg ctaaggaatc
      241 acgaatatcc cctgaacgct ccgcttcata tttaagcgct agctctgttc ctaagatttg
      301 atcaattgaa tggatcaatt caagtagcgt agtgcttttt cctgtaccga cattgaattg
      361 ctgtcctaaa gctttttctt ccttggcaac aagtaaaagt gcttgtataa catcttctat
      421 ataaacaaag tctctagact gactcccatc gccaaataaa gtaaattccg ctttttctcc
      481 tgccaattgt tttttgtaac gatccactag aatcgaaatg actccgctat atggagagtt
      541 tggattttga tttggaccat aaacgttaaa aaatcggaca gcactagtcg gaacatcata
      601 taaatgacag taatccaaaa catattgttc tgctgcaaat ttatcgatag catatggtgt
      661 taatggacgg atgactgatt cttctttttt tggcagtgtt ggttcatcac cataaactgc
      721 agctgatgaa ctaaatacta atcgttttaa attagattga tatttccgtg ctaactcaag
      781 caataaaagc gcactgtcaa aatttacacg atgtgtttct acaggccgtt ctacagaatc
      841 tgccacacta gctacagcag ctagatgaaa aatataggca aactgatatt ctttcatgat
      901 tctttccatc aattgagaat ctgatacatc cccctcaatg aaagtcactt gtttatccat
      961 atccaagttt tctttttttc ccattgataa atcgtcaact actacgattt tgttttcttt
     1021 tcccaaataa ttcgctagcg ttgaaccgat aaaaccagct ccgccagtaa tcaatatagt
     1081 gtccatcaaa ttcactccaa ttgcacattt tcgtatcaaa taatattagt ttaccatttt
     1141 tatattgtaa aaactataaa atttgttgaa gttgggataa ctagttttct tttctacttt
     1201 catataccta aaaatagttt caattagtta ctccttcttc tttagatata gttttttgga
     1261 tagaacccag atatccccaa aaaataaatg aacctatcgt actaaccaaa atgagttctg
     1321 tgataaataa tgccgaaact gctattgtaa gaatcattaa aatagcaaaa aagtgcttat
     1381 cttttactag atttggttgt gtgaaacaat aacgcaaggc tttaaagcat tggataaata
     1441 agaaggttaa caatgtcaat agacctaatc ctcctgtagc agccatcact aagaaaataa
     1501 aattatgagc agttaaagta tcttttccca ttaatgtata tggtagttta tcatgtgcat
     1561 attcaatata gtagcctgga gaacctccgg atatcggctt actcttgaat atttcaattg
     1621 cacttttcca aagttcaaac cttaaattag aaacatcccc gctatcagtg tcagggcgtt
     1681 ttaaatcaac tttattgctg ctagttgttc ctgatgaatc agatgtatct ttttttactt
     1741 ctgtaatatg cggaacatgt gcccatgcat gagcagttat atctgttaca taatacatcg
     1801 caattatact tagcaatcct actactaatg cagttagcca tctttttaaa gtattagttt
     1861 taagaaaacg actactataa cttgtaaaga aactaaaaac aaaagttgtt attaataaac
     1921 aaactaatgt ggtgcgtgaa cccgataaag caataaacga gaattgaata aaaatattaa
     1981 ttacaaaaaa tattttaaat agaagattta aatgcttgta agtgaccaaa taatatatag
     2041 caataatgat gacgattaat gatataaccg caccaaaatt tggatctgta tatacaccaa
     2101 ataaacggtt gcctaaccac cctaaacgaa ctgggtaata cttatatttt attggaattt
     2161 ttatgccata ttgaatgaca aaccagtata atgatactaa attagctaaa aaccatacca
     2221 atgaaagtat ccaggtaaat actttgaaaa aaaagttact actatagtct ttgccaaact
     2281 gataaatcat aaagaaaaag attaattgcc acatgattaa cttaatattt tcgctatatc
     2341 catattttaa attcattaaa ctgcttacta gcatcgcgcc aataaaagca attaatagcc
     2401 aatcatattt ttttagattt ttttctctaa atgcaataat tgcatctata atgatcaaga
     2461 caactcccgc tattccagca ggccaaaaaa gtaaagtatc atatctagta ggtaatagta
     2521 aatacgggcc tattgctcgt aacattaaaa taaaaccaaa ataaataaaa taccaacctt
     2581 tcacatattt taaaaatctt tcattcaaca tttttcctcc atccacctat ttctcataaa
     2641 tatttcttat cctttttagg ataaactggt cctttcaatt ctaacaaaaa aatgctaaac
     2701 taacaatgta tattaatgaa tttcgagagg tgaaagatga aaagagtaat tacttacggt
     2761 acctttgatt tgcttcatta cgggcatatc aatttactga aacgagcaaa acaatacgga
     2821 gactatctta ttgttgccct ttctacagat gaattcaatt ggaatgcgaa acaaaaaaaa
     2881 tgttatttta gttatgaaaa aagaaaacag ttacttgaag ctattcgcta cgttgattta
     2941 gttattcctg aagaaaattg ggaacaaaaa gtaaaagata taaaagaata ccatgcagat
     3001 tatttcgtga tgggcgatga ttgggctgga cattttgatg aacttgaaca actaacagat
     3061 gcacaagtga tttatttacc aagaacacct gagatctcta caactcaaat aaagaacgaa
     3121 cttaattctt taaatagtga taaataatat tttttaaaca ataaaagaac aatatatctg
     3181 gataagacgt aaaaaaatgg ctctataatt tctaggactg atagaatttt ctgtcggtcc
     3241 tagatttttt gtaaaaaaaa acagacataa aaaaattccg atagaataga gtcgtctaaa
     3301 acatcaatct gaaaggaagt ttttgatatc catttctcat tctatcaaag agctcttcac
     3361 tattaaagat aaaaatattg aaattaattc ttttcaagaa actttttata aggacaagaa
     3421 agccatcgta atcgaaggtc cattagttcg cacttttaga cattgtctat tttgtaaaga
     3481 atctgcaaag cagatcgaga aaaacggcaa gaagatttca atgattatct taaatcgcag
     3541 tgggaataaa caaacctatt tgcgtcttag aaaacaacat taccactgcc aatcatgcgg
     3601 gaaatatttt actgctcata cgtatcttgt cgctccttca tgttttattt cgaaacaagt
     3661 tcattacacg atattagaag agttgaccga gcgtcaagca ttaaagacaa ttgcgaaacg
     3721 ttgtgatttt cagtaacgac agttcaacgg acattatctg ctctatcggc aacgctgcga
     3781 cagaagctag actatttacc tcattgttta ctattcgacg agtttcgctc tctttcaatt
     3841 ttccatggga agttctcctt ttcttgtatg aattgagaga cagggaagct ctttgacatt
     3901 ttgtccagcc ggagaaaaaa aagatttgat tgcttatttc atgcgtttta caagaaaagc
     3961 tcatatgggg gttcgataca ttgtcacaga tatgaatgcc ccttattttt cattagtcaa
     4021 agaacccttc ccaaatgcac aaatcatcat tgattgcttt cacattgggc aacacttgaa
     4081 caagtttttc gatcgtgttc gaaaacgtgt gatgaagcag ttgaatcaaa aatgtcaacg
     4141 gcacctttaa aatgtaggaa aacggcaatt taaaattgtt ggtttaccta catttttgta
     4201 tgctcttctt atcccaaatg aaatggaggc tcagggatga gaaaagacat acgcgaagga
     4261 gtgatgatct acgtgatcaa tgaaatcaaa cccaattatg ctgcactagc aaaacaatac
     4321 gactgcgact accgaacagt taagcatgct tacgaagaag cccaagttaa agaatcaaaa
     4381 cctccagaac gtaagaagcg tccaagtaag ttagatccct acagagaaat catccaagac
     4441 aaaatcaaag accaatgcag tgcttacagc attttcaagt ttattgagca caaaggattt
     4501 ggaggaagct acagtttagt aaagacctat tgtcgccaga ttaagcaggc gaaagtacac
     4561 aaggcaaccg tccgagtcga acatagccct gggctttctg ctcaggttga ctggaaggaa
     4621 gatatgcgac taatcagtaa tcagggcgaa atttttgagt tcaatatctt tttgtacgtc
     4681 ttgccatatt ccaagaaaaa atatgtcaca ctaactttcg accgaaaaca agacacactc
     4741 tttgagtgct tagatgatgc gttttatcaa accggtggcg ttcccaaaga aatctggttc
     4801 gataatatga agacagtcgt tgaccattct cgtagtcagt tttccaaacc gcatttcaac
     4861 aaacgttttt atgctttcag taaggatgcc ggcttcacac caattgcctg tcggccattt
     4921 cgtccgcaaa caaagggctg tgtcgaagct ttagccagaa ctgttgaccg cctacgggtc
     4981 tataatcatg aattttatga tgccgttgag ctgtgtcatt tagtgaataa cctttgtgtt
     5041 gagttaaata acgaggtttc tcaatcaact gatcaaacac cagatgaacg ttggcagacg
     5101 aacgaaaaag agcacctgca tccataccaa aaaaaccttt tgaatcccta ttttgaagac
     5161 tttattcgcc gtaaagtcac caaggaagaa atggtcattt tccgtaaatg caggtactct
     5221 gtggcgcctc gctatatcgg taaagaagtc gaaatcgaac tctccgataa agaagaccaa
     5281 gtacaaatct attataacgg agaactgatt cggtcgcatc cattaacgac aaaacagctg
     5341 aactaccacg aggaagacta tttccaaatc ttgaaatccg atgtcatgag tcacaaagaa
     5401 gacgatggaa tcagagcgta tactcgtgaa agtctcaagc aatatgatct ggtggaggtt
     5461 gccgatgaag aatagccatt accaagaact gatgaccaac ttagagactc ttggtttgaa
     5521 tcatatgcgg gaatatgttc ctaactacat cgaagtggcc aatcgagagg agctttcttt
     5581 aacggaagct cttctcgagc tgacacgcag agaagttgat catcaaaata agcaaaaaat
     5641 gaatcgtgtg gtggcacgcg cccgcttttc caagagaacc agcctggaag agtttgattt
     5701 tgccttccag ccaagtatca ataagcgcga agtgctagac ttaaggcaca tgggctttgt
     5761 ggaaaaacat gagaacttgg tttttatcgg cagtcctggt gtaggcaaga cacatctgac
     5821 gattgcattg ggaatcgaag cctgtcgtca aggctatcgg acactattca tcacctgtca
     5881 tgagctgctt ttgcgtctgc gaacagccta tgaaaaaggt accctagagc gtgttctcaa
     5941 acgatacgct cgctatgacg tcttgattat tgatgagatt ggctatcttc cgattcaaaa
     6001 acaggaagct gatttattct ttcagcttct tgccatgcgc tatgaacacc gctctacgat
     6061 cattacgacg aacattgtct tatctcgttg gggggagttg ttccaaagtt ctgagatcgc
     6121 agctgccatc cttgaccgtt tagtacatca cgtcaaggtg ttcaagatca caggcaagtc
     6181 ttatcgtatg agagacaaga tctaaagtgt cgattgtcga caattactat tgccgaaaac
     6241 ctacattttt atcctgccca aaacctacaa ttttaagctg cccttgacat caaaaagaca
     6301 cttcacaagc taagtgctat cgacaattga aacctttgta taaattactt ctataatcag
     6361 atgacgagtt ggattataca acattcaaga aatggcagaa ttttcaatgg cgctatttaa
     6421 cagaaagtga agtagttgat tgtctaccct caatctctta tgaacttaaa atggcttagc
     6481 agtattatca aaagctgctt aacaattatc acaagaaaca gctagctact tcgagaaatg
     6541 accaaagaag ttccctcgaa acttcactat cttaaaaagc ctttttcata tctgaaaaag
     6601 gcttttaact agcattttcg aaaaagtatt ttaatacaaa attagagaat cttcgcacac
     6661 atatcaaaac attgaaacga tttgcttatg gattttgcag ttttaccaat atgcgcttgt
     6721 ggatttttct aatcaacggg ttaataagct atgcattaaa agctagtaaa tcttactaac
     6781 tttgttgatc ataaatgacc ttatcagccc gatttgacaa agaactactt ttttacaaca
     6841 ttattttatt ttcgaataat ctttcttact tcatttcgta tatctggaaa aagaaaataa
     6901 acaaacggaa gataaataat gaaaatcatt attgatttaa ttgccatatc taaaaatgaa
     6961 gaagagaata attgtggaag aaagtaatag gccgaagtag tcaaagtaag attgactata
     7021 aaaaataaga caacgttctt cccaaagaaa taggatattt ttatttcttt tcgtacaaaa
     7081 aatactctaa agaaaaagac taataattct actaccacga gtacgattgt agccgctaca
     7141 gcgccataag taggcacaaa agctacattt ccaataatac ttacaatcgc accaatgatc
     7201 attggataag catactcctt atctttttct atagcaattg caaattggtt agcaaaaact
     7261 ccaccaattg gactaacaac tataattaga caagaaataa gcatgtttat tgtcatcggt
     7321 ttgaaattct ccccaaaaaa ccatggaaca aattcttttg tattgataaa caccacacta
     7381 aaaagaatta atgcaataat aatcgtgtaa tcaaaagatt tttttgtgta aactaaaata
     7441 ttttctgatt ttccctctgc ctgtgcttta gatattttag gcaacataac aatagtaatt
     7501 gatgttaaga ctgccaatat aatacgagca attttttgag actgatcata ataagataat
     7561 gctgttggat ccacaaaata cccaactatc gttttgtcta aagttgtgta cacttgcata
     7621 aacaactgag gagtcaaaat aataaaagct ggtttccaca actgtttaaa aacattagag
     7681 ttaaagagtg gtttacctac ttttccgtgg atactcaacc agaaaactaa acttcctaaa
     7741 aagttagtaa ttgaagtaat gaacacataa atccacaaat catctttagt tgaaacaaaa
     7801 aggattatta acaataccga tgataatttg ataactgtat ttctaataac cactttcgaa
     7861 aattcttcaa ttccttgaaa aaaccaagaa atatctaatg ttagccctgc aataaatatc
     7921 aattgaatta agtataaatt tttatacttt acatcaaaaa aataaatata ggctatatag
     7981 agaaaggaaa cactcaagcc tattaaaaat tgaagaaagt aaatattcca gaattgtaaa
     8041 gatagtgctt ttttatcttt ccttgattta gcgatatttc gaatacgaaa aggattcatt
     8101 cccacagcaa tgacagtcat taaaaattgt gatattgaga aaacataccc ataaatccct
     8161 aaaccagttg aacctattct acgactaagt ataggtatag taagtaaggg caaaacaatc
     8221 aaaaagactt gatatatcgc attataaaat atatctttta ctgttttttt cactttatat
     8281 ccctctctaa caactcaaga accttttctg tacttgcccc tgtttcataa tttccaatat
     8341 aatcaagtaa ctcttgagaa cttttcaaaa gcttttcttg ttggttttta aaaacatcaa
     8401 caaggccata actatcttgt acgcttataa ttggtaactc acttaactgt atataaaaac
     8461 ctctttctaa gctatattct ttttcatcat agttaaaaag aataattggt ttttttgtta
     8521 gcgcaaaatc gaaaaaaata gatgaataat ctgtgattaa aacatctatc tcttcaaaaa
     8581 gcatttcaag aggaatccca tcattaatta aagtaatttg atgttttttc atatattcta
     8641 tacttttaga atcagttgct aatttactat gaacattagg atgaaattta gtcattaaat
     8701 ttaattcttg ttgttgcttt ttaaaaaatt cacgtaagtt atccacacct aaccattcta
     8761 gttgcgtaat tccgttgtcc tcaatattat ttcgaaaagt tggagcaaat aagacattga
     8821 tttgtttctt tttcaagtca aacacttgct ctttactaga ggctaattca tcgtttctag
     8881 gagttcctac ttctaatatt ttagaaggaa cgatattgaa tgctgaaaca tacttctcag
     8941 aactatagcg attaccagat aataaatatg taattgactc actatctttt ttcgcatagc
     9001 ttaggtaacg tttaggtaac ttatcttttg catcaaattc aatttttttt aaaggcgtac
     9061 cgtgccatgt ttgaaaataa aattgttttt tcctttttgc atacttaatc gttttccgac
     9121 tatcgtctat ccaatactta gctgtataca aataaaaggc agctaaaaaa gaatcggaat
     9181 caattctttt aatataatct ggtaaatcag ttataatcgc ttcttttttg attaaccaaa
     9241 taattctgta attcaatttt ttttcatgaa ttttctctga aataactctt ggatttccag
     9301 aataattacg cccgccaaaa cttgaaaata caattaaatt tttttgaata ggtaatattt
     9361 taataaaaac cgggaaaata ttccttgaaa aaaatttttt tatattcttc atctcttacc
     9421 tcacagattc tatcttaatc aaaaat
//
LOCUS       DAMEET010000090         9388 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.091, whole genome shotgun sequence.
ACCESSION   DAMEET010000090 DAMEET010000000
VERSION     DAMEET010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.091"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            2..77
                     /locus_tag="PB780_002168"
     tRNA            2..77
                     /locus_tag="PB780_002168"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            79..149
                     /locus_tag="PB780_002169"
     tRNA            79..149
                     /locus_tag="PB780_002169"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Gly,seq:tcc)
     gene            163..236
                     /locus_tag="PB780_002170"
     tRNA            163..236
                     /locus_tag="PB780_002170"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:197..199,aa:Ile,seq:gat)
     gene            261..348
                     /locus_tag="PB780_002171"
     tRNA            261..348
                     /locus_tag="PB780_002171"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:295..297,aa:Ser,seq:gct)
     gene            370..441
                     /locus_tag="PB780_002172"
     tRNA            370..441
                     /locus_tag="PB780_002172"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:403..405,aa:Glu,seq:ttc)
     gene            464..553
                     /locus_tag="PB780_002173"
     tRNA            464..553
                     /locus_tag="PB780_002173"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:500..502,aa:Ser,seq:tga)
     gene            566..639
                     /locus_tag="PB780_002174"
     tRNA            566..639
                     /locus_tag="PB780_002174"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:600..602,aa:Met,seq:cat)
     gene            658..730
                     /locus_tag="PB780_002175"
     tRNA            658..730
                     /locus_tag="PB780_002175"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:691..693,aa:Asp,seq:gtc)
     gene            734..806
                     /locus_tag="PB780_002176"
     tRNA            734..806
                     /locus_tag="PB780_002176"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:767..769,aa:Phe,seq:gaa)
     gene            811..881
                     /locus_tag="PB780_002177"
     tRNA            811..881
                     /locus_tag="PB780_002177"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:843..845,aa:Gly,seq:tcc)
     gene            894..967
                     /locus_tag="PB780_002178"
     tRNA            894..967
                     /locus_tag="PB780_002178"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:928..930,aa:Ile,seq:gat)
     gene            979..1050
                     /locus_tag="PB780_002179"
     tRNA            979..1050
                     /locus_tag="PB780_002179"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1012..1014,aa:Glu,seq:ttc)
     regulatory      1239..1318
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            1316..2509
                     /locus_tag="PB780_002180"
     CDS             1316..2509
                     /locus_tag="PB780_002180"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDE0734207.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            2639..4111
                     /locus_tag="PB780_002181"
     CDS             2639..4111
                     /locus_tag="PB780_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDE0734208.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4194..4982
                     /locus_tag="PB780_002182"
     CDS             4194..4982
                     /locus_tag="PB780_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDE0734209.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5049..5717)
                     /locus_tag="PB780_002183"
     CDS             complement(5049..5717)
                     /locus_tag="PB780_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HDE0734210.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5832..7544
                     /locus_tag="PB780_002184"
     CDS             5832..7544
                     /locus_tag="PB780_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDE0734211.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            7547..9319
                     /locus_tag="PB780_002185"
     CDS             7547..9319
                     /locus_tag="PB780_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDE0734212.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2767 a         1643 c         2122 g         2856 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgccatgc gggtgtagtt tagtggtaaa accacagcct tccaagctgt
      121 tgtcgcgagt tcgattctcg tcacccgctt ttagtttttt atgggcctat agctcagctg
      181 gttagagcgc acgcctgata agcgtgaggt cgatggttcg agtccattta ggcccattag
      241 tacaaaatct tattggtttt ggggaagtac tcaagtggct gaagaggcgc ccctgctaag
      301 ggtgtaggtc gggaaaccgg cgcgagggtt caaatccctc cttctccgtt tcaatatttt
      361 gttttacctg gcccgttggt caagcggtta agacaccgcc ctttcacggc ggtaacacgg
      421 gttcgaatcc cgtacgggtc attaatttaa tatagtttta catggaggat tacccaagtc
      481 cggctgaagg gaacggtctt gaaaaccgtc aggcgggtaa aaccgtgcaa gggttcgaat
      541 cccttatcct cctttttcat attatcgcgg gatggagcag tctggtagct cgtcgggctc
      601 ataacccgaa ggtcgtaggt tcaaatcctg ctcccgcaat taaaaactaa aaacattggt
      661 tccgtggtgt aggggttaac atgcctgcct gtcacgcagg agatcgcggg ttcgattccc
      721 gtcggaaccg tttggctcgg tagctcagtt ggtagagcaa tggattgaag ctccatgtgt
      781 cggcagttcg attctgtccc gcgccattat gcgggtgtag tttagtggta aaaccacagc
      841 cttccaagct gttgtcgcga gttcgattct cgtcacccgc ttttagtttt tatgggccta
      901 tagctcagct ggttagagcg cacgcctgat aagcgtgagg tcgatggttc gagtccattt
      961 aggcccattt attttattgg cccgttggtc aagcggttaa gacaccgccc tttcacggcg
     1021 gtaacacggg ttcgaatccc gtacgggtca tggaagatac tttttgagta tctttttctt
     1081 tttatctgaa agaatgattt taaaaagctt agcagagtta tctttatgga tgattgtgct
     1141 aagctttttt ggacagttct aatgtaaaat atttttatat tttttaagca aaaaaatgaa
     1201 aaaatacgag cagaaaactg ttttatctgc tatacttggt tcgttgcaag ttcccgaaag
     1261 gatccgcagg acggagatgc cttgtaaccg aatttaacta gggggaatca ttataatggt
     1321 agaaagacac ttatttacat cagaatctgt ttctgaagga catccagata aaattgctga
     1381 ccaaatcagt gatgcaatct tggatgcaat tttaaaacaa gatccaacag cacgagtagc
     1441 gtgtgaaacg tctgtaacaa ctggtcttgt tttagtattt ggagaaatat ctacgacggc
     1501 atatgtagat attcaaaaag tcgtacggga aacaataaaa gaaattggtt atacacgtgc
     1561 aaaattcgga tttgatggag atacagcagc tgtattggtt gcgattgatg aacaatctcc
     1621 tgatattgct caaggagttg atgaagcact tgagatccgg gatgaagata aaaaagacgt
     1681 attagatgag attggtgctg gtgaccaagg tttaatgttt gggtttgctg tagatgaaac
     1741 accagaattg atgcctttac caatcgcttt gagtcatcgt ctggtacgac gcttggcaga
     1801 tttgcgtaaa tcaaatgaat taacgtattt acgtccagat gcaaaatctc aagtaacggt
     1861 tgaatatgat gatcaaggac aaccggaacg cgtagataca atcgttattt caacacagca
     1921 tgatgatgca gtagataatg aaacaattcg tcatgatgtc atcgaaaaag tagtgaagga
     1981 agttattcca gctgaattat tagatgatca aacgaaatat tatatcaatc cgactggccg
     2041 atttgtcatt ggtggtcctc aaggggatgc cggattaaca ggaagaaaaa tcattgttga
     2101 tacgtatggc ggttatgctc gtcatggtgg cggtgctttt tctggtaaag atgcgacaaa
     2161 agttgaccgt tctgccagct atgctgcacg ttatattgct aaaaacattg ttgcagcagg
     2221 gcttgctcgc aaagcagaag tacagctagc ttatgcgatt ggtgttgctc aacctgtttc
     2281 gatctcaatc aatactttcg gtactggaac ggttccggaa gaagaactga ttgctgcagt
     2341 gagagaaaac ttcgatcttc ggccagcagg aatcatcgag atgcttgatt tgcgtcgtcc
     2401 gatttacaaa caaacagctg cttacggtca ttttggtcgt acagatgtag atttgccttg
     2461 ggaacatacg gacaaagtag atgcgttgaa agcaagttta gcaaaataag ttatattcag
     2521 ctggaacgat cgccaagtgg tggttgttcc agcttttttt atggcagtta acctcttgaa
     2581 aaagatagta attatttatc tatttttaga tcctgtcata atgaaaggga ggaataagat
     2641 gaaaaaagaa acaaatgtta ctttggtcac agtgagtatt ttcattgcaa cgttcatgac
     2701 ggctattgaa ggtacaatcg taacaaccgc tatgccaacg attgtcggtt cattacatgg
     2761 aatggaaatc atgaactggg tattttcgat ttatttgctg acaaacgcta tgcttactcc
     2821 gatttacgga aaacttgcgg ataaagtggg acggaaacca atctttattt ttgggacatt
     2881 attgtttatt atcggttcgt tattttgcgg tttatctcaa agcatgattg ggttgattgc
     2941 tgctcgtgcg attcaaggga tgggtgctgg ggcaatgatg ccagtagctt tgacgatcat
     3001 cggcgatctt tattctattg aaaagcgagc aaaagtttta ggtttgaata gttcagcctg
     3061 ggggattgcc agtgtttttg gaccgcttgc gggaggattc atcgttgaaa cgatcagctg
     3121 gcactggatc ttttttatca atgtgccgat cggaattcta ttaattattt tgatttggct
     3181 atatttaaat gaaccaaaaa tcatacatga gtctaaacca atggatattt ttggtagttt
     3241 tagtttgatg gccatgcttg tctcattgct gttagggttc caattattag gagacgaagg
     3301 aatgagtccg cttgtttctg ctttgctgct aggtagtatc gtaatgttag gtatttttgt
     3361 ttttgccgaa aaaagagcta aagatcctgt catcatgatg catttattca aaaatccact
     3421 atttgttgtg gtcaacttgg ctgcagcatt ggctagtggt tttttgatgg gaatcgatgt
     3481 ttatatccct atgtggatgc aaggtgtatt agggctgaat gcagcaattg gtgggctggt
     3541 attagcacca ttatcactag tctggatggt cggttctttc cttgcaagca agtggatgac
     3601 tcaaatgaaa actcattggg tcttgagaat tggactacta atcgcagcta ttgggggcgc
     3661 ttggttgtat cttatgcctt tcagaagtaa ctatttatgg ttttttgcta tatcaacggt
     3721 catcggtata ggcttaggtg tgaccatgac tacctcgact gtcagtgcac aaaatagtgt
     3781 atctcgtgac cagcttggtg tagcaacttc ttttaatacg ttagttcgaa caattggtca
     3841 aacagtcatg gtttctattt ttggtttatt gataaatggg gtaactaaac gagaacttgc
     3901 agcagcaaat ttaacaaatg atgcagatat catgaataaa ctggtgaatc cacaaacggc
     3961 taaatatatt gatgcggata ttttgttacc attaagaaaa attctttatg atgggttaca
     4021 ttccgtttat ctagttggct tgttactcgt gatcattgct ttggtcttaa cgcttttcgt
     4081 tcatgaaaaa agtggtaaaa taaaacagta gtttcggtta agatatgtac gtagccgaat
     4141 cattcaaatt taaagttatt caaaaataac ttcttactaa ggaaggcgaa aatatggcat
     4201 ttattcaagc aaatatttat tcgaatgtgt tagaaatgga agtcaatcta aatgtgatac
     4261 taccgcaaac aacaaaaaag gtgattggca ccagttcaga gcaagagaca acggatatac
     4321 ctgttttata tttacttcat ggaatgggtg gcaatcatag tgtatgggaa agacgtacgt
     4381 cgattgaacg ttatgtagca aactacggat tagctgtcat catgccatct actgatcttg
     4441 gctggtacac agatacagtt tatgacatga agtattggac attcattgca gaagagctac
     4501 ctgagatttg tcatgaatta ttcccgcaat taacaagaaa aagagaaaaa acatatgccg
     4561 ctggattatc gatgggcgga tatggcgcgt taaaactagg ccttgcaaaa tcagaaaatt
     4621 tcgcagcagt aatctctctt tcaggcgctg tttctgtagg aaatcgaatg gatgatcttt
     4681 tgatggttcg taaagcatct ttttgggaag ggatctttgg accattggat caaatcgaag
     4741 gttcaaaaaa tgatccgcaa tacttattga aagaactagt aaaaagcgga aaaccagttc
     4801 caagatttta tatggcttgt ggggaatctg attttcttta tcaggcaaat caggaaatgg
     4861 tatctgctat gcaggaacaa ggcttggcgg tcacctttga acaaggacct ggcgatcata
     4921 actggatctt ctgggaccaa tggattcaac gtgcattggc gtggctaatg gaagaaaaat
     4981 aaaaaaagag tcttaaaaag atagctccta tagagatcac aaacatagtg atctctatag
     5041 gagctttttt actgctcgtt ttctcttctt ttattttgtt ggcataagcc ttcaatatag
     5101 gtttgtgcac tcgttagaaa gagtgctttt tcattcaatt ctgcatggga ctgcatactt
     5161 tctttaaatc cctctaattg attttcaact acataattac gcataaatag aactgtttct
     5221 ttcaaaaatt catttccgtc cataattttt tgtcgaagct gctgttcatg ctgctgatcc
     5281 attaactgtc caatcaataa attgtggaga gaggcaatgg cctgatgact ttgggtgact
     5341 gaaaatcctt cacgaacaag taattggatc atttgctcca aatgttctaa tcgactcgga
     5401 aaagcgggca cagtattgat ttctaattct gccccacaag ggaaacgtgt ataaaggtca
     5461 tagtaatttt ccatatactg cagcaactgt tcattccaag gcaaagttgg atctggaagc
     5521 gctaattctt cttccattgt ttcagccatt ttttgcaaga gctgttgttt gtttttgaaa
     5581 taccaatata aagcaggtgc ttgaacatga attctgtttg cgatgtttcg catggaaagc
     5641 tgcgaaagag cttgtgtttc agccaggata tcaaaggcgg cctgaatgat gattgcttga
     5701 gaaagcttta tttccatttt atcaacctcg tgtattgcat tatcttttta aacatggtaa
     5761 attgttagtc ggtctaattt tacagtgtta aattagtcct gtcaatttaa acaaaggggt
     5821 gtaatcaacg aatgaaatta atcttacgac atgcgaaaaa atataaagta gctgttttta
     5881 tttccttgct gtccgtagca gtcatggttg cagcagcatt atggcaacct aagttactac
     5941 agcaagtatt ggaagctatt attacagaag ataatgatga aatgaaaaca attggtattt
     6001 acctgatcag tctggctttg ctaggattag ccgcaggtgt aacgaacacg attttctctg
     6061 caaaagtagc tcaaggcgta agtgcagata tccgagaaga agcatttcga aagatccaga
     6121 cattttcatt tggaaatatc gaacagttct ctgctggaaa tcttgttgtt cgtctaacaa
     6181 atgatatcac gcaaatccaa aatttagtga tgatttcttt gcaatcactt tttcgaattc
     6241 cattcttatt tattggtgcc ttcattttag ctatgatcac gatgccgcaa ttatggtgga
     6301 tcatcgtagc gcttatcgtt acggtatttc tgattacggc actttcattt acaaggatgg
     6361 gcaagcactt catgatcatt caaaaactga ttgatcgtgt gaatagcatt gcaaaagaaa
     6421 acttactagg gattcgtgta gtgaaatctt ttgtacaaga agaaaaccaa ttgaagtcat
     6481 tcagcaaagt aagtgaggac ttaacaaaac acaacatcat agtcggtaca ttattttctg
     6541 tgatgatccc gtccttcatg ctggctgcca atttggctgt ggttggggcg atcttttttg
     6601 tgagcgatct tgttaaagat gatcctacgt taattggtgg aattgcttca ttcatgaact
     6661 acttgatgca gattatgatg gcaatcatta ttggcggaat gatgatgatg atgacttcaa
     6721 gagcagccgt atcgctgaag cggattgcgg aaattttaga tgctgaacca gatcttactt
     6781 atttagacgt tccagaacaa gagttgacgg gttctgtcac ttttgaccat gtttcttttc
     6841 gttatcctgg tgaggataca gatacgctta aagatatcag cttttctatc aaacctggag
     6901 aaatggttgg tatcgtcggg gcgactggtg ccgggaagtc aacactagca caattgatcc
     6961 ctcgtctatt tgatccgaca gaaggcaaag ttgaagtagg tggtgtagat cttcgtcaag
     7021 taaatgaaaa gagtttaaga caaacggttt cctttgtttt gcaaaaagca attttattct
     7081 caggaacgat tgctcaaaac ttgcgccaag ggaaaaagaa tgcaacagaa aaagagatgg
     7141 aacatgcttc ttctattgct caagcaaaag aattcatcga aaagttatct gatcgttacg
     7201 aagcaccaat agaagaacgt agcagtaact tttctggtgg tcaaaagcaa cgtttatcca
     7261 ttactcgtgg cgttatcggt aatccaaaga tcttgatcct agatgacagt acgagtgcac
     7321 tggatgctcg ctctgaaaag ctggtccgag aagcactcga tcgagatttg aagggaacaa
     7381 cgacgattgt catcgcacaa aaaattgctt ctgttgtcaa agcagaccgc atcttagtat
     7441 tggatgaagg cagattagtc ggcgaaggaa cccatgagga attggtagct ggaaaccgtg
     7501 tctatcagga aatctttgaa actcaaaaag gaacggagga gtaagtatga ctgatctaat
     7561 aaaagcaagt aaatttttct atcactattt gaagcggtac aaactctcct ttttctttat
     7621 ctttatcaca gtgatcattg ctacctatct acaagtaaag gcacctcaat acatcgggga
     7681 agcttttcaa gaattagcta attacgtagg aggcttgatg caaggcgtag acgataaaag
     7741 caaatttgtt tctgtaatat ggaaactctt gctgttttat gtactagcta gtgcagccaa
     7801 ctttatctat agtattttgt tcacacaagt agtagggaag tcaacgaacc gaatgcgtat
     7861 cggtctattc aacaaattgg aaaaattgac gatccgtttc tttgattctc atcaagacgg
     7921 tgaaatttta agtcgtttta ccagtgactt agataatatt caaaatagtt tgaaccaagc
     7981 tttgcttcaa gtcatcacga atgctgtttt acttgtcggg atcttgatca tgatgttccg
     8041 ccaaaatgta gagttagctt gggctacaat tgcttccact ccagtagccg ttttgatagc
     8101 tgtcttgatt atccgcaaag cacggaaata tgtggatctt cagcaagatg aagtggggaa
     8161 actaaatggt tatatggatg aaaaaatcag cggacaacgt gtcatcatca caaatggctt
     8221 acaagaagaa acgattgaag gattcttaga gcataatcaa agtgttcgcg aagcaacctt
     8281 caaaggacaa gcttattctg gtctactatt tccgatgatg caaggaatgt cactggtcaa
     8341 tacagcgata gttatctttt tcggcggctg gttagcatta aatggtgatt tggaacgttc
     8401 ggtggcatta ggcttagttg tgacattcgt ccagtattca caacaatatt accaaccact
     8461 gatgcagatt tcatctggat acagcatgat ccaattagcg attacagggg caagacgatt
     8521 gaatgaaata tttgatgaaa aagatgaagt caaaccagaa aacggtaaag catttgatgg
     8581 tgttgaaaaa ggtcttgcgt tgcagcacgt agattttggt tattctccag aaaaactagt
     8641 cttgaaagat gtctcgatca atgtggacaa aggagaaatg gttgctcttg ttggtccaac
     8701 aggatcgggg aaaacgacga tcatgaatct gctaaaccgt ttttacgatg tcaatgatgg
     8761 ttcagtagtc atcgatggaa tagatatccg agattatgat cttgatgcat tacgtagaca
     8821 tgtaggaatc gttttgcaag aatctgtatt gttctccgga acgatcaaag acaatatcac
     8881 attcggcaaa ccggacgctt cagatgaaga agtcatcaat gcagcaaaac aagcgaatat
     8941 tcatgacttc atcatgacgt tagaaaaagg ttatgataca gagatatccg aagaaaataa
     9001 cttattcagt acaggtcaaa agcagttgat cagtattgct cgaacgatca tcacaaatcc
     9061 atctttattg attttggatg aagcaacaag taatgtagac acggtaacag aagcaaggat
     9121 ccaaaaagca atggatgaag caatcaaagg aagaaccagc tttgtgattg ctcaccggct
     9181 gaaaacaatc ttgaatgcag atagaatcgt tgtcttgcgt gatggggaag tcattgaaga
     9241 aggcagtcat catgagttat tagcagaaaa tggattttat gcggaacttt atcataatca
     9301 atttgtattt gaaagttaaa ttaaaagaac ctcgttataa cggggttctt tttatatatt
     9361 ttatgttgtt tgtttttata aggggcag
//
LOCUS       DAMEET010000091         9124 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.092, whole genome shotgun sequence.
ACCESSION   DAMEET010000091 DAMEET010000000
VERSION     DAMEET010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.092"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            13..636
                     /locus_tag="PB780_002186"
     CDS             13..636
                     /locus_tag="PB780_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734213.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1086..2135
                     /locus_tag="PB780_002187"
     CDS             1086..2135
                     /locus_tag="PB780_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDE0734214.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2132..3013
                     /locus_tag="PB780_002188"
     CDS             2132..3013
                     /locus_tag="PB780_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDE0734215.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3016..3786
                     /locus_tag="PB780_002189"
     CDS             3016..3786
                     /locus_tag="PB780_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDE0734216.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4065..4919
                     /gene="aroE"
                     /locus_tag="PB780_002190"
     CDS             4065..4919
                     /gene="aroE"
                     /locus_tag="PB780_002190"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDE0734217.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5097..6260
                     /locus_tag="PB780_002191"
     CDS             5097..6260
                     /locus_tag="PB780_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDE0734218.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6377..6787
                     /locus_tag="PB780_002192"
     CDS             6377..6787
                     /locus_tag="PB780_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDE0734219.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6961..>9124
                     /gene="topB"
                     /locus_tag="PB780_002193"
     CDS             6961..>9124
                     /gene="topB"
                     /locus_tag="PB780_002193"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDE0734220.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENS"
BASE COUNT         3140 a         1567 c         1978 g         2439 t
ORIGIN      
        1 gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca agaaatgaat
       61 gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg tcttgttttt
      121 ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga aaaaatccga
      181 gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt gaaacctttt
      241 gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt gatgttcagc
      301 aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc tgagcttatg
      361 gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca tgaactggaa
      421 caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg aggaaaagaa
      481 gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga gttgatccct
      541 gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt ggatagaacg
      601 ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat gaaacggtgg
      661 gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc aaaataacaa
      721 ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact taaatcaatt
      781 cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag aaaacaaaaa
      841 aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc atagatacaa
      901 ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt gactgccgca
      961 tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc cattgtcttt
     1021 ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt tgcaggagga
     1081 aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt tatttagcag
     1141 gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg aaccagacaa
     1201 aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta gatgagattt
     1261 atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa aatcttgaga
     1321 tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt caacagctag
     1381 tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa tcattagcaa
     1441 acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg ggggctggat
     1501 tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat cagccgcctg
     1561 ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa gtcggaatct
     1621 tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa gaagctgcaa
     1681 aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag attgcacaaa
     1741 cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat aataccattg
     1801 ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc gtcattccat
     1861 ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat caatatcaat
     1921 tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa aaaccggaaa
     1981 cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct caagctgatc
     2041 gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt acagatgact
     2101 cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt cacaaggcat
     2161 gctttgggca atattaggat tagggatttt catgacttat cggatcttag actttcctga
     2221 tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag ctattactca
     2281 tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga tagcaggact
     2341 cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag ggattttagt
     2401 gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc tttctttatt
     2461 gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt actttgacac
     2521 agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat tcttcttcaa
     2581 tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg ctcggtcgtt
     2641 agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg gtgtcattgc
     2701 attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata aagggattgg
     2761 cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc aagaattgac
     2821 gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc tattgatttt
     2881 agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg ctatcatctt
     2941 agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa cgggttttga
     3001 gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa agtcgtaaac
     3061 aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat cagagaaggg
     3121 gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt caatacgatc
     3181 gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct gatcacaaaa
     3241 gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc caagatggga
     3301 acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag aggtcaaaaa
     3361 cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag actatgtcaa
     3421 gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt atctggtggt
     3481 caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt actattatta
     3541 gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg attaacaaat
     3601 cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat ggaagacgcc
     3661 ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca agatatttct
     3721 gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga agaagtagtg
     3781 gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca ttgacatctt
     3841 cctttgattc atcctatact taaaacaata aacaattaaa catatttggg tgttttgtag
     3901 gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg acaagtaaac
     3961 gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc gtatcaaacg
     4021 ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt tctggacaaa
     4081 ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca ttgatgcaca
     4141 atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa gtaaatccaa
     4201 tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt gtaaatcttt
     4261 cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag gaagcgcaat
     4321 tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc tataataccg
     4381 atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa aagaaaaaaa
     4441 tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct gcgttagacg
     4501 gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa gtaaaagaaa
     4561 cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac tttgccgata
     4621 cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca acagatatcg
     4681 ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg catgagaatc
     4741 tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa gaagcaaaaa
     4801 aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga gcaatcgcgt
     4861 ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg tttgattaaa
     4921 aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa aagtaatcct
     4981 ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa tgataggctt
     5041 ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga accacaatga
     5101 gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag cgggatacgg
     5161 ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag gaagatgcta
     5221 cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac gagcaacaga
     5281 tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt ttcaatcggc
     5341 aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa atcattctat
     5401 cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt aaatatgtag
     5461 ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg caaaaagcga
     5521 cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa gatgccgggc
     5581 gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg aaactgattt
     5641 cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag atcaattcag
     5701 cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat tttggaatcg
     5761 attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc gatgccttat
     5821 tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt gtagatatta
     5881 aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt ttccgaatgg
     5941 atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac gctattttca
     6001 gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt aaagctttag
     6061 gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt tcgattgcac
     6121 gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg aattatgggc
     6181 aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa tctgctatta
     6241 cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca aggcttttgt
     6301 catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga tttaaccaag
     6361 agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc gtgaagccat
     6421 cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat acggtgaaat
     6481 gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca cgaatgcacg
     6541 gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa ttggtcctga
     6601 attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag aagaaacgaa
     6661 gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg atgctgttcc
     6721 gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc agattaacca
     6781 agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg gatcatcgtt
     6841 tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt tattgccaga
     6901 tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg aggtcagaag
     6961 atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc cgaaaccttc
     7021 caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt tgctggtgaa
     7081 gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg agcttatgaa
     7141 gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac cttccgttac
     7201 gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt gcagtcagcc
     7261 gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc gtggtcgatt
     7321 attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg gatcaattct
     7381 cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa gaactatttt
     7441 ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg aatgaatgga
     7501 agtccattat atagcttgct acttcaacga aaagggattg atggcagttt ttctcttggt
     7561 cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat caaacacttc
     7621 aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg gcagttttct
     7681 ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa ggaaatagaa
     7741 aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac gaaaaaggaa
     7801 aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa gatgaatcag
     7861 ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta cgaaagcaag
     7921 tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca tcagtatctg
     7981 gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca gacaactgag
     8041 atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca tgcaattatt
     8101 ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt acagcaagca
     8161 atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta tatctacgaa
     8221 gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg aaagatccca
     8281 ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga agctcagact
     8341 cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat ggagaaagaa
     8401 acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa aactgcggga
     8461 aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg aatagggaca
     8521 gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat cgaagtcaat
     8581 aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt agccaatgaa
     8641 ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag aaaaattggt
     8701 aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat cctacattta
     8761 ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat tagcgggaca
     8821 cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc aaaatgtaaa
     8881 aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga atgcgacttc
     8941 aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag agatttgctt
     9001 tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg gaaattcgat
     9061 gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg gatcgaaaac
     9121 agtg
//
LOCUS       DAMEET010000092         9100 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.093, whole genome shotgun sequence.
ACCESSION   DAMEET010000092 DAMEET010000000
VERSION     DAMEET010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.093"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            298..906
                     /locus_tag="PB780_002194"
     CDS             298..906
                     /locus_tag="PB780_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDE0734221.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            1074..2711
                     /locus_tag="PB780_002195"
     CDS             1074..2711
                     /locus_tag="PB780_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDE0734222.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELYLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(2783..3895)
                     /locus_tag="PB780_002196"
     CDS             complement(2783..3895)
                     /locus_tag="PB780_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDE0734223.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(3970..5343)
                     /locus_tag="PB780_002197"
     CDS             complement(3970..5343)
                     /locus_tag="PB780_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDE0734224.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            5564..6181
                     /locus_tag="PB780_002198"
     CDS             5564..6181
                     /locus_tag="PB780_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDE0734225.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6279..6734
                     /gene="argR"
                     /locus_tag="PB780_002199"
     CDS             6279..6734
                     /gene="argR"
                     /locus_tag="PB780_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDE0734226.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            6752..8254
                     /locus_tag="PB780_002200"
     CDS             6752..8254
                     /locus_tag="PB780_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HDE0734227.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            8349..8555
                     /locus_tag="PB780_002201"
     CDS             8349..8555
                     /locus_tag="PB780_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734228.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            8641..9042
                     /locus_tag="PB780_002202"
     CDS             8641..9042
                     /locus_tag="PB780_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDE0734229.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
BASE COUNT         3140 a         1681 c         1875 g         2404 t
ORIGIN      
        1 agaatttgat aaaaatcaaa aatttttttg ctttttttta tggattattg cagtgaatgt
       61 tacagaatct gcgtttttca ttcaaaaata aaaaaacata gaaaaacgat tactttgctt
      121 atacaacaag gcttttatgg ttttgtatca aaaaaatatt tgtttaaatg ttacaaatat
      181 taaataattc gttattttgt cattaaatgt catgtttctt ttacctatga aatatttcta
      241 aatgataaag ttaatagcgt cgaaagaata aaaaacggag aaaaaaagga gtttaatatg
      301 aattcattta agaaaattgt tttaggtaca acttttgctg ctggtgctac tgcaatgttt
      361 gtaggaacta caaatgctca cgctgatgaa gtttatacag taaaatcagg tgattcatta
      421 tctaagatct cacaaaaatt tgcaggagac aactcaatga tcgatgcaat tgctgaaaaa
      481 aattcgatcg caaacatcaa ccgcatttat gttggtgaac aattaacaat cccaacttca
      541 aatgattctt cagcaacaac agaaaacaaa acaacagaaa atacagcatc tacaactgaa
      601 acagcaacgc aagaacatac atacgttgca cctgttgaaa cagttgaagt agctcctgct
      661 gccccagctg ctgcaacagc gccaacttca agctctgcaa aagaatggat cgcacaaaaa
      721 gaatcaggcg gttcatacac agctactaac ggacgttaca tcggccgtta tcaattagat
      781 gcttcatact taaacggcga ctattcagct gccaatcaag aacgtgtagc agaacaatat
      841 gtaacatctc gttatggttc atgggatgct gctaaaacat tctggttagc aaacggctgg
      901 tactaagccg ataaaaggaa aatgatgaaa ccgagaaact tgtttattca agttctcggt
      961 ttttttgtgt gaaaaataac tcgtttgctt cccacagcgc agcagtacaa ataaaaactc
     1021 gaataatgta taataaagaa aacgtttttt tgtttaggga atgaggagga gccatgagta
     1081 caaaagagag tctcgaatta aacgaaatca caagtggaaa tccactgtta tccagtattc
     1141 gatcgattgt agaaacgaca ttttacggta ataatgttca tgaagttttt gataggaaga
     1201 cagcttatca actagcaaaa gggagtccag gaacgatcat cacagattta acaatctcac
     1261 atgctgaaga actatattta ccagctgatg ttaggacact agtattcaat gatggaagca
     1321 ttgtgggaag aacagcaagt gctcggcgga tctttgagga cttagacaag gaacaaagca
     1381 aatacgagaa aattttaaga gaagctgtgt atcaaagtcg caagcgccag ttttaccata
     1441 caaaagtaat cgttggccta tctgaagaat tttctgtgca aagtcatttg ctgattccag
     1501 aaggatttga gaacaatttg tattcttatt tgctgaattt tcagccagcg aattcgaaga
     1561 tcttagagac ttatcaaaag tctgtttcct ttgatgaggg agatctttat atcttttgtg
     1621 atcccgaatg gacacatgaa acatttccaa atggactcat tttgtttgat gctctccata
     1681 atacagcggc tgtgctagga ttacgttact ttggagaatt aaaaaaagcg actttgacgc
     1741 ttgcttgggc attgggaaat cgcaatggct ttattgcctg ccacggtggg atgaaacaat
     1801 atgctagtaa tgaccagacc tatacgatgg ctgtatttgg gttgtccggt tctggaaaat
     1861 caacgattac cttagcaaaa catacggaag gtcactctgt ttcgatcttg catgacgatg
     1921 ctttcgttat ttctcgaaaa aatggttttg ctactgcctt ggaaccttct tactttgata
     1981 aaacacaaga ctatccgtct gatgatccgg ccatttcttc tttccttact tgtcagaatg
     2041 tgggcgtgac atgtacaagt aaaggagaaa aagtcctagt aacaggagat atacgaaacg
     2101 gaaatgggcg gacagtcaaa tcacgttatg cgacacccga ccgagtcgat catcttagcg
     2161 aaaagatcga tgctgttttt tggatcatga aagatgattc gcttccgcca attatcaaga
     2221 tcaatgaccc agttttagca gcgtcttttg gtgcaacatt agcaacaaaa cgatcagacg
     2281 cggaaaatat cactacaaaa agacagctag atgagctagt cattgaacca tttgctaatc
     2341 ctttccgtgt ctatcctctt agggaagact atcaagcttt tcgagagttg tttttgaaac
     2401 agcaaacagc ctgctatgtc ctgaatactg gatatttcga tgataaaaaa atcaaacctg
     2461 aaacgacatt gtcaatcgtt gaaagaatcg tgcaacaaac ggcagaattc aagtcgtttg
     2521 gtccatttga agacatccac tatttaccta taccagattt tcctgtttct tttgaggata
     2581 cgtcctataa aaatctggtg aaacaacgac ttgatattcg tttacgattc attgaagctc
     2641 agataggaaa agatctcttg ccagatgaag caaaagaaag tttggagaat ttgattcaga
     2701 aattgaacta aaataacagc tttttcaaga agtaatccat gcgcaatatc gctcgggatc
     2761 cttacttgaa aaagctgttt ttctatgatg gatttttcgt aatataggtg gagatgtcct
     2821 tttgataatc ttccgtaggc caatgaccag tatttaagaa gttggctaat agatagccga
     2881 tataaggacc tgtggtcaag ccagaagagc ctagaccgct agcagccagt aagcttggat
     2941 catctggcag tacgccgaaa aatggggaaa aatctgacgt ataagcacgg gtacctacac
     3001 ggaactcgta tggccagttt tttatctgat ctggatcagt caagaactgt tctgtactct
     3061 tagtcaatcc cttataagct tcgctagtag gagccaaatc ccagccttga tcgttttcat
     3121 gggtagcacc taaaaggatc tttccatcca tgaaaggaat gaaatcagct tctccgtcaa
     3181 gcatagcaac aggccagtct tgactcatct tataagatgt ctgaaatgct aagagctgcc
     3241 ctttttgcgg acgtatatct gcagcatagc ctaattcttg taataaaggc ttaagtgccg
     3301 ggccaggtgt caaaattaca cgatcagctg tgtattctcc aaacggtccc ttgatgagcc
     3361 aagaatcgct cttcttatgc aaagtgactt ttgcctggat ctgctccatt tgtttttgtg
     3421 ctaaacgctg accaagatgt tctaaatatg cttttccatc cagacgacca ccaccactga
     3481 tacgcagtga aactgctggt ttcaataacg gaagtgcttg agatgtctgt tcaggtgaaa
     3541 gccattcgat gtttccaatt tctggcgctt cttcttttcg tttctcggca agaacagcca
     3601 attcagctaa atctttttca ggtcgaagca aaagtgtccc tgattgtgcg taaatcgttt
     3661 gatcaagttg gaaatctttt ataagtttag ggaaaaaagc agcgccgtct ttagccagac
     3721 gataccattg tttgtttctg cgtttcgaca accaagggga gataatccca gcgctagctt
     3781 tagttgcctg tcctgtccct tcatcaaata aagcaacatt gaattttgat gtatctaagt
     3841 aactggctag ggtcatacca atgatcccgc caccgataat ggcaattttt tccataaaaa
     3901 agcctccttt tacaaacaac cattttcaca caaaaaaacg gcagatgcaa cacctgccgt
     3961 atatggaatc tacgcttttt tagcaaagaa cagataatag gcgaatcgag caaagcttac
     4021 gagaagcaac agcaacaaga cgcctaatgc tgcttttgat cctaatgtag tagacacttc
     4081 atagttatgg gaagtatgat gctggacgta attgataatg accgctacta atgaagtagc
     4141 cacagcgcca gaaaactgct gcaaggtatt gaacattgcg ttcccgtctc cttgtagttc
     4201 ttcattcagt gcattcattc cggtggtcat catattgctg taagataatc cgataccgat
     4261 catataaaac acatgacaag caatcaatac acccactgaa gcgtgtccta tgagtaggaa
     4321 taatgctagc caaccaatca aagaaagaat cagcccagca aaaattggct tcttcggacc
     4381 atattgatcc agcattttcc cggaaaaagg agaaagcaaa gcaccaaccg ctgcgccggg
     4441 aagcatcgct aatccagcga caaaaggact acttccttga acgatctgaa ggaaattcgg
     4501 caagacaaaa gatacaccaa gtaataaaaa ctgataaact aagaagccaa tcaaaaacaa
     4561 gcggaatgtt ttatttgaaa gaatagacag tcggatcagt ggatgctcca cttttttact
     4621 tcgatagtaa aaaaggaacc agccaatacc gccgatgatt aaagcaatcc agctggaaac
     4681 tgttcccaac gtactcaaaa atgttagact tccactaaac aaaagacaaa ttcctaaaac
     4741 gcttatccaa tccaaataac cacttttttg tactgccatc tcgggaatag caaaccagcc
     4801 gataatcaaa gaaagaaaca aaacgggcac aagaaacagg aagatatgat tccatgtaaa
     4861 atgtgaagtc ataatgcctc cataagtggg gccaattgct ggagcaatgg ccgttgtcaa
     4921 agtaccgaca cccatcatcg cgcctcgtcg attgaggggg gcaaatgtca agataatatg
     4981 gaacatcaac ggaagagcga tacctgtcgc tgcaccttgg agcaatcgac caaacaatag
     5041 gaccatgaag gtaggagaga agaaatcgat cattatacct gccaagaaaa gcagatttga
     5101 agctaaaaag agttttttga gactaaaatt gcgattcaaa taagtagaca gcggaacaat
     5161 gatcgaaatc atcaaaagat aaatcgttgt tacccattgg acttgtgcag tcgttaaatc
     5221 aaactgtctg ataagagttg ggaaagttac attcatggcc gtctcgatca atacgcccgc
     5281 aaaagacatc atccctgttg caattactgc aggtactaaa agcagctgtt tttgttttgt
     5341 catttacatc acctctaaaa aatttcgtaa aaaaagagag tgatttctat tataaaaata
     5401 gaaaccgcct ctctctcgac acgccgaaac gtgttatctg tacgatttat ttacttcact
     5461 tattctagca caaactgaaa aatagtgtca acacgattag ccaagtcact caaaaagagg
     5521 tatgataata aataactaaa tggaatttcg cggaggaaga aaaatggaaa aaattgaatg
     5581 gcgtaaagat aagagctata gtatgaaaaa aagcccgcaa atactagagg ttcctaaaaa
     5641 atcattcttt agcatcagag ggatcggcga cccgaatcag gaagacttta aacgaagagt
     5701 agcctgtcta tatgcagtca gctatgcaat acgcatgtcg cctaaaaaaa attggctgat
     5761 tcctgattat cagatgtata ctgtctatcc actggaagga caatggggac tgcaagaaaa
     5821 gtatttgcat gaaccagtga tgaaaaaaga acatttttct tatcaattga tgatccgaca
     5881 gcctgacttt gttacagaag cgatagcaca agaagccatt cagcgttcta gcaaacttcc
     5941 ggaagacttg aaagaacaag tagtattcaa aaagatggaa gaaggtcttt gtgcccaaat
     6001 cttacacgtc gggccctatg ccgaagaacc agcaacgttt gagaaattag aagcattcat
     6061 cgcaaaagaa ggctatgaaa gagcttctaa agaacacaaa gagatttaca tcagcgatcc
     6121 aagaaaatca gcacctgaaa aaatgaagac gattttgcgt gttaaaatag aaaaaggtta
     6181 gcaacctgaa tgaaaacaaa aaataaaagt aaaaaatacc aaaatatgaa gaaatattac
     6241 ttatcttttg ctataatcaa cattgagagg tgtctaatat gaaaaagaaa cagcgacaag
     6301 cactgatccg ccagatcatt acagaacaac ctattgggac acaggaagag ctgcttgcgc
     6361 ggctgcatga agcaggcgcc gatgtgactc aagcaacgat ttctcgagac attcgagaaa
     6421 tgaagttagt aaaaagtcaa aatgaaaaca aaatagtacg ctatacatta tttaaccagc
     6481 catctgtttc tttaaatgaa gaacgtttgc gtacagcaat tagacgcgag gttttgcgta
     6541 tccagtctgt tcagttcatg gttatcgtat tgactgaacg aaatggtgcc gatgtggtaa
     6601 cgaattggct agatgaagtg gcttaccctg aagttgtcgg aacgatggcc ggtgtagata
     6661 cattcattat tatttgccgc tcagaagaag aagcacagcg atttgcagaa aagctggaaa
     6721 aaatgaggga ataaatgagg aggactttta tatgaaagat atgaaagcgc tgcaaaatgg
     6781 atcggatatt cgaggaatcg cattaccagt tgaaggatat gaagtaaact tgacgacaga
     6841 catggtaaaa aagatcggct ggggactagc aaactggata aagaaaaaga agaaatcaac
     6901 cgatccaaat aaaaaatgga cgattgggat tggtcatgac agtaggataa gcggaccagc
     6961 actgaaagag gcgttgatcc aaagtctgaa agaacaaggg atacatgtca tcgatttcga
     7021 attagctact acgcctgcta tgtttatgtc cactcaattt gaacagttct cgtgcgatgc
     7081 tgcggttatg ctgactgcta gccatcttcc ctattattat aatggaataa aattctttac
     7141 ctcagaagga ggagcagaaa aagaagatat tgcttttatc ctatcacagc agcaaaaaat
     7201 aaaaagcgaa caaactggaa gcgtagaaaa agcagacctt ttgacacctt atgcgcaaga
     7261 tttagtcaca aaaattaaaa aggggattgg aacactagaa agcaaaccac taaatggttg
     7321 gaaaatcatt gttgatgccg gaaatggtgc tggtggattc tttgctgaaa aagtattgaa
     7381 tagcttaggt gcggattcaa caggttccca atttttggaa ccagatggcc gatttcctaa
     7441 tcatatccca aatcctgata ataaagaagc gatggaaagt atcaagcagg ctgttttagc
     7501 aaatgaagca gatcttggga tcattttcga tacagatgtg gatcgttctg cagttgtcag
     7561 tgcttctgga acagtgatca atcgaaataa tttgatcgca gtattaagta caatcgtatt
     7621 aaaagagcat ccaggaacaa gaatcgtgac gaattcccct acttcttctc atctacaaag
     7681 attcattgaa gaaaaaggag gcatccaagt tcgttatatt tccggctatc gaaatgtaat
     7741 caataaagca ttagaactta accgtcttgg gaaagataca cagctagcca tcgaaacaag
     7801 cggacacgct gcattcaagg aaaactattt tctggatgat ggtgcatatg tcatcgcaaa
     7861 gatcctgatg cttttgccag aattaaaaag aaaaggtatc acattagatg aacaaatcaa
     7921 agagttgaaa cagcccgtag aagtacaaga aatccgatta aaattgttca gcgatgaacc
     7981 acgcgaacaa ggaaacaaga tattagaaga aatgaagcga ctgttggaag aagaaaaagg
     8041 gatgaacttc gacccggaaa atgaagaagg tattcgatat gatctagaag agccatacgg
     8101 aaacggctgg tttttgatcc gtttaagctt acatgagccg ttactagtat tacagataga
     8161 aaatgatgaa ttcggaaaga acaagttggc gttagtaaaa ttagcaaaat tattcggaca
     8221 tttttctgat cttgaatcat taacagcaga ataaaccagt ctttttgaaa ggctagttgc
     8281 ttggacggca aaaaaagtgg tatggttaaa tcgagtgctg gtagggagac cagcaaaagg
     8341 aggaaaaaat ggtaacgaat atggttattt ttttaacagt attggtttat ggggtcaact
     8401 ttctggtata tatgtatctt aaaatcaatc atataaaaga ttatgtagaa aagattgcta
     8461 tttattttgg aacaaatatg acgctgttgt tcacgagtag tatttttttg ttttttggaa
     8521 aaatagtaga agacggcatt ctggtattcg aataagcaga atttttgtag aaagcgcctt
     8581 acttgtcata cactataatt atagccaagt agcaaatggt caatcgaaag gagcagtaca
     8641 atgaaaaaag catacgaaac agcaatgatc aaccatggcg ggcgcaatgg agaagtcgag
     8701 gcaccaaatg gctcgatgca tatgaaaatc actcctccag gaatccatgc agaaggaaca
     8761 aatccagaac aattatttgc agcaggttat gcttcttgtt tcaatggggc ggtacaacat
     8821 atgatcaaag aagcaggaat gacagcagag tccacagtaa aagcacgtgt ctctctttat
     8881 aatttagatg atggcggcta tcagatcggt gtagtattag aagtgcatat cgatggcatc
     8941 agcgaagaag aagcccaaaa aattgcagaa gatgcgcatg aattctgccc ttactccaaa
     9001 gcaactagag gaaatatcga tgtagaagta acgattgttt agaagaataa aggaaatcga
     9061 accagactaa aaacgtgaaa ggaccgcttt ttgtctggtt
//
LOCUS       DAMEET010000093         8872 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.094, whole genome shotgun sequence.
ACCESSION   DAMEET010000093 DAMEET010000000
VERSION     DAMEET010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.094"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(75..797)
                     /locus_tag="PB780_002203"
     CDS             complement(75..797)
                     /locus_tag="PB780_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDE0734230.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQSYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKTVPIDHLLIETDGLHAAEWALGGIFSEKYYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(787..1524)
                     /locus_tag="PB780_002204"
     CDS             complement(787..1524)
                     /locus_tag="PB780_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDE0734231.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYRNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            complement(1536..2528)
                     /locus_tag="PB780_002205"
     CDS             complement(1536..2528)
                     /locus_tag="PB780_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDE0734232.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQETKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDITYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDNPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            complement(2543..3286)
                     /locus_tag="PB780_002206"
     CDS             complement(2543..3286)
                     /locus_tag="PB780_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDE0734233.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     regulatory      complement(3360..3464)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3527..4849)
                     /locus_tag="PB780_002207"
     CDS             complement(3527..4849)
                     /locus_tag="PB780_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDE0734234.1"
                     /translation="MKTYDAIVIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYFVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANVVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRNPPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     gene            complement(4836..5801)
                     /locus_tag="PB780_002208"
     CDS             complement(4836..5801)
                     /locus_tag="PB780_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDE0734235.1"
                     /translation="MTKLIEFKHVQKKYDRKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFNEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            complement(5798..7336)
                     /locus_tag="PB780_002209"
     CDS             complement(5798..7336)
                     /locus_tag="PB780_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HDE0734236.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKVLNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            complement(7752..8039)
                     /locus_tag="PB780_002210"
     CDS             complement(7752..8039)
                     /locus_tag="PB780_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734237.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            8173..8841
                     /locus_tag="PB780_002211"
     CDS             8173..8841
                     /locus_tag="PB780_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDE0734238.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
BASE COUNT         2548 a         1865 c         1511 g         2948 t
ORIGIN      
        1 attaatcgaa cggtttactt ttatacaaag aaagccaaaa agaggtgtga cacaactagt
       61 gtcacacctc ctttttattt acctaatcta atgaaacgtt ccaagttttg ataagtattt
      121 ttcttaactt cttcaactga tagctctttg atctcagcga ttgtctttaa ctgttcttca
      181 agcagtgaga cataatattt ttctgaaaaa atccccccta aagcccactc tgctgcatgt
      241 agtccatccg tttcaataag taagtggtcg attgggactg ttttagctag ttgccagact
      301 gctgggttct ttttcaaatc aagaccaatg ctgaaataac agcctaaatc aatatagtct
      361 tgttgatagt ccaaagaaga ataccaatga atcaaatagt gattagggta agattggata
      421 taatttaaga tctctttttc gcatcctttt gtatgcagta caacaggttt ctcatttatg
      481 gctgcaaaag caagctgtcg ctcaaatact tttttttgtg tagtcatcgg tacattcgtc
      541 caaatattat ctaaccctat ttcaccgatg atccttgctt ttttcaaaaa aggctcaact
      601 tgttcaaacg tatagctatc tgctttccaa ggatggatac cgtaacttaa ggcttgagtc
      661 ttacttgctg caagctgtct gttttccttc cattcttctg gcgaatcaca attgatgatc
      721 gtcaaaatcg tgtgttcctg ctgaaggagt gctaaactct gactcccatc taaatgacaa
      781 tgagcatcac ttaacatcta attcacctgc caataactga taaatcgttt gcttcaactc
      841 tgattgctgc agtagccatt cacgtgaccg tcgtttttca ggcacttgaa tctcagcaat
      901 cacatgtgcc ggacgggcgg aaagcacaag aatcctgtct gataaataaa ttgcttcttc
      961 gatatcatgc gtcacaagta aagtagtccg ctttgctttt ttaggcagct tcaacatcca
     1021 gtcttgcatc tgccctctgg tcatagcatc taacgctcca aatggttcat caagaagcaa
     1081 gtaagaagaa ggattcatga ttgttcgtaa caaggccgtt cgctgcttca tcccgccaga
     1141 taattgttcc ggcaaaaaat gttcatatcc gtttaaccca aagatatcca gataatccct
     1201 tacttgttct tcattgattg ttagatgatt gattttttga tataacgtaa cgtttcgata
     1261 gacgctcagc caaggaagta acatatcttc ttgcggcata taactaaatg ctgtatggag
     1321 accagttact ttttgatcgt ctactttgat cgttccagaa gtcataggct ggactcctgt
     1381 taaagttttc agcaatgttg acttaccggc tccgcttggc gcgacaatcg atgtaaaggt
     1441 atctttaggg atagtgaaat ggatgtcttc taatattttc aggttttcaa tgactacatt
     1501 caagtggctg atttggattg acatatctat cactcctatt ttataaactg attagtgaat
     1561 agttcttgcg aaggtacatc ttgcttgatc aatccgtttt cctgcataaa agctgtataa
     1621 ttgcgccaaa ctttttcttc catcaatccc caattctcag gattatccgt ataatttttt
     1681 gacaagaatg cttgtgattc ttctaaaaac gtccgatcat tttcttttgc atatttttgc
     1741 aacagtttag cactgtcatt tggatcctta atagcttcct gatagccttt tttcgtggca
     1801 tccataaaag actgtaccaa ttctggatca cttttgatca gttgatcatt cgtaataatt
     1861 accggagtat agtaatctaa ccgttcatct aattctttca atgggatata gtttagttca
     1921 atacccgctt cttttgcttt gatcatatcc caaccttcaa agtaccattg gatatctgag
     1981 gattttccta agctttctgg cccttctcca ttgcttccta ccattgtcag ttttgaaaag
     2041 tccccgccgt ctttttccat cacagctttt aagacagctt cttcacttgg actttgccaa
     2101 cctgcatatg tttttccttc aaaatctttt ggtgaatgga tgttactgtc tgatagggta
     2161 gcgaatccag aagtattatg tttgatcact gtagcaattg ctttgactgg tatattctgt
     2221 ccatctgcgt gagcataagt gatatcttct tgataactta caccaaattg tgctttgtcc
     2281 gcacctacta gaccaatact tgtcgaatta tctcctggtt cgatgatttg gacatttaaa
     2341 ccagcctctt tataatagcc tttttctttt gctaggtaga tgccagtatg attagtattc
     2401 ggtacataat ccaaaacaag tgtcactttt ttcaaatcct ttgtttcttg tttgctactt
     2461 gtttcttttg cagtgccaca agcggataaa agcaacagta gcggtaataa aagccataat
     2521 tttttcattt gatccccttc tcttaaaaaa tataaagcat acgataatac ccttttttca
     2581 ataatgaaca agcgccattc agcaataaac tcaatagaat gacacaaatg atcgcagcaa
     2641 aaactttatc gatctggtag ctgtttttta cgcgtatcat gtaataccct aagcctgctt
     2701 gagcacttag ccattctcca acgactgctc cgctgacaca ataagtagca gccactttta
     2761 aaccggaaaa gaagccggat aaacccgctg gtattttata tattcgataa atcttccctg
     2821 tatctgcccc catcgttttc aaaaagatga tttgctcttt tgatactttc ctcaatgtat
     2881 ccgaaaaaga aactacgatc ggaaaatagc ttatcaagac aacaaggatc acttttggaa
     2941 gcataccgaa accaaaccat agggtaagta atggccctaa caccatgatt ggcaacattt
     3001 gtgaaatcac tagtaacgga taaaaagaca tttctaaaaa cttccaataa tccattgcca
     3061 aggcagtaat aaaagaaaga acgctagcta ttaccaaacc aagcactgct tcatttaatg
     3121 tcgtgacaga atgggaaagt aatacttgtg catctgccgc taatgcagaa aatacagcag
     3181 caggtccggg taaaataaag gcaggtattt tccaaactat gacaacaatc tgccaaataa
     3241 ttaaaatgaa tgccatactg actgctggat aaagattttt cttcattttg ttacctcctt
     3301 tcttctaata tgaataacaa aaaacgcttg aaatgggcac gacatagtaa cccgcttcaa
     3361 gcgtctgcta tgtttctccc ttcaccagta ttaactgaca ggttcaatgg gtctaagttc
     3421 acaacttact ctcatgtcgt ctacgacatc cccagaaaca ggtattcatt ttttaaagtg
     3481 tagcacaaga aggaaaaagc aacaagcgct ttagttatca tgctgattat ttcaataaat
     3541 aggagagatc acttgccaat gtcggatacc ccatgatata gctatccaac tcttttttag
     3601 tcaatttttg attgatgact agtgtcaaat catcgatcaa ttcgtctgct tgctcgctaa
     3661 tcactgcagc accaatcaaa taattttgct ggtcaaaggt caattcagcc ttagcaactg
     3721 gttcattgac acgatgatag gtgaaccaac tggtaagatc catctctacc acttgatctg
     3781 acgaagaagc gtgacttttt gtaacaccta cttcagcaag tttaggactg gcgtagacga
     3841 tcgtaggtat aatcggatat ttgataggtt cgcttgttgc atccgttatc cgcttagcta
     3901 cataatttcc ttcaaaagtg gcgactggtg tcaatttggg ggggttcctt gaaacaatat
     3961 ctccacacgc aaaaattttt ggattgctcg tttgcagata atcattgact gcaattccgt
     4021 gtttatcaaa gacgacattc gcttgctcaa gtgctaaaga ttctgtattc ggctgtctcc
     4081 cagtcgcaca aaaaatcata tctgctacta attccgtctc ttttccgact aaacgatagc
     4141 gagtaccttc tgaaatgatc ttttgggttt caacaccaaa agcaaactga atgccagatg
     4201 cttccatttg atggacggct tcctctacca aagacgcctc aaattctttt aatggccgtt
     4261 gattatgatg gacaatggtt actttgctcc ctgcagcatt ggcaatcgta gctagctcaa
     4321 aagcaatata accaccaccg ataaaaatga tttctttagg cagtacaggc aatgataaaa
     4381 agtctgcact agttttcaag tattcctttc cctccactgg taagatcgta ggtctttgcc
     4441 cggttgccaa aacaaagtaa tccgcatgga agacttcttc gttgacttca atagatgaat
     4501 catccaaaaa acgagcggtt cctgaaagat gatcgatttc agcttctgct aattgttttt
     4561 ttcggctttc tgggactgga tccgtaaatg ttcgtttgaa tttttgaagt tcttcccagt
     4621 tggcagttgg gatctcgtta aaaccttttc cactcaattg ttttacccga ttacgtgcct
     4681 ctactgcact taacaagact tttttaggat cacatccacg gttaggacaa gtcccgcccc
     4741 atagatcttc ttcgaccact agtactgttt tacctgcttc tttcaatcca taagcagcac
     4801 ttaagccgct cacacctgaa ccgatgacga ttgcatcata tgttttcatt tccttcctcc
     4861 tctcgactta aaaaagcaaa gatttcttta cttgtcagcg acccaatact tctttgcttt
     4921 tctactatca caatgcttgg atacaccgaa agaagcgaat atacttcttt cataggcgta
     4981 tcaaagcaaa cttgttgacg agcattttct ggtatttcat ttaagaaata tccattgacg
     5041 actaaatctt ctactcgcca ctctttcgtc tgtttcggtt cattaaagaa atgccgaacg
     5101 tagtcgtttt taggattcat caataactgc tctggtgtgt cacattggat aatttctcca
     5161 tccttcataa atgcaatacg atcacctaac ttgattgctt cttccatatt gtgcgtaacg
     5221 aaaacgatgg tcgtgcccaa ttcttcgtgt aaggacaaaa ccaattcttg taatgagttt
     5281 cttgaaatcg gatctaatgc gctaaatggc tcgtccatca aaatcacatc tgggcttgca
     5341 gcaatagctc ggatgatccc gatccgttgc tgttctcctc cagacaactc tctaggcata
     5401 cgatctcgat aaatatcagg agataagcca actttttgaa gaagttcatc tacacggtct
     5461 gctcttttct gcttttccca acctaacatt tcagggatga cttcaatatt ttgcttgact
     5521 gtcatggtcg ggaacaaagc gatttgctgc agcacatatc ccatattcca ccgcattttc
     5581 tgcagattat attcatttaa cgattttcct ttgaagtaga tgtctcctgc tgatggttta
     5641 gataatccat tgatcatttt caaagtagtt gtttttccac taccggaagg accaaccaaa
     5701 acaaagattt ctcctttttt gattgccaga tttaggtcat cgatgacata ctttctatca
     5761 tacttttttt gcacatgttt gaactcgatt aatttagtca ctgatcttcc tcctttacta
     5821 atccttcatt tttcaggaag attcgtgcta cttctgtcgg ctttttcttt tcgacattga
     5881 cttggtaatt catatcaatc atttgatcct ctgttatttt tccgcttaat ttattcagta
     5941 ctttaacgat ttcaggatgt tctttagcaa aatctttttt catcagcggt gcgccttgat
     6001 aagctgggaa taaccctcga tcatcttcta gagtcactag atcgtactcc ttgagctggc
     6061 tatctgtgga ataggcatcc acaatattga catctccatt attgattgct tggtaacgca
     6121 aacttggctc catgctttgt acacttgaaa aattcaagcc atatgcttct tggattcctt
     6181 tatatccatc tgaacgatca ataaattcca gtgtgaatcc cgctttgatc tgatcagaga
     6241 tagctgccaa atcggaaatc tttttaagtt gatgttcgtt cgcaaaagat ctcttgactg
     6301 ccaaggcata cgtattttgg taagccattg gttttaataa tgtcatatcg aattgcttat
     6361 tcaataattc ttctgcttcg tcatatgttt tttcttttgt caaatcagca cttttcattg
     6421 cttcaggtac cttcaccaag ctttctagga cagtacctgt gaactcagga tagatatcga
     6481 tttgaccttg atccagtgca ttgaacaaaa aactcgtctt gccaaaattc ggttttaatt
     6541 caacttctgt ttttgtgttg tcttcaatca attctttata catttggatc aggatttcag
     6601 gttccgctcc taattttcca gcaatcgtga tggtttcatt tttaaacaag gcagactgat
     6661 ataaagaaac gccgccaaat aaacacacca ctgctgctaa agtaatgatg gaatatcgag
     6721 gtttcttatg ctgcaaccag cgaatcagtc cgctgaatat aattgcaagc aaggcagaac
     6781 tgatcgcacc aatcaatgtc aaaacaggtg tatttcggtc aatacctaac aaaataaatg
     6841 tccctaatcc acctgctccg atcaatgctg ccaaagtagc cgtaccgatg attaaaacta
     6901 aggcagttcg gatacctgag ataatgacag gtaatgcgat gggaagctct actttcagca
     6961 agcgtctcat tctggacata ccaaatgcta ccgctgcttc ttcaatagac gggtcaattt
     7021 cagctagacc aatatacgtg ttttggaaaa tcggaagcaa agcgtaaata acaagtgcaa
     7081 tcaatgctgg aacagttccg atccctacaa atgggataag taaacctaat aaggcaagtg
     7141 aaggaatcgt ttggagcaca cccgtgatct gcaataagac ttctgccatt tttttatgat
     7201 tggctgccca aatcgctaaa ggaatagcaa tcagtgctgc aatcagcaaa gaaatgagag
     7261 aaatggacag atgttgaaat gcagcttcca gcaactcgcc ttttctttca cttaatgttt
     7321 gtataaaatt attcatgcga ccttcctttc tgttattctt taagtataaa catttctttc
     7381 ctttttccca actaacttgt cttgccttaa ccttacagca cgaacaaaaa aaaagaaaaa
     7441 aatacctcta aatatgctga caaaaaagaa tccaagcaac aaagtcggat caactttttg
     7501 gttctcgttt tttcactacg aataccataa aaatctatac gaaattgtca cttggaatca
     7561 gctatatctt ttttacacac acaactttta ttttaataaa aagaaacagc caataaagta
     7621 gaaatctcgt gggggagaac acctttattg gcctgttcaa cgaatattat actgtatcat
     7681 gacacttttt tgccaaaaat gtaaaaaacg gttgttttga catcataaac gtcttgtttt
     7741 cttattttta cttatatcac cactttattt tctatctctt tcactccata aagcggaatg
     7801 agttcttctt ttgactgata ataaacagct ttctcacggt tcttcatcaa aaaaagaaag
     7861 atgtctttca tagcaagtgg tgctttgccg tgttcaaata aaaagataaa ttgatttgct
     7921 ttatttatct ggaccattgt cacaggcacc agtttttgtt cttgagccaa aaaaactcct
     7981 tgatactctg tttttttggg tgattttgta agaattgtca ataagtctgc tgctcgcatt
     8041 tttccactcc ttatttgata aataaaaaga aacagccaaa aataattttt tttcattttc
     8101 ctcttgaagt aagagtataa gtcagtataa taaagaagtt gctgaaaatt ttcaaaagaa
     8161 aagaggctag aaatgaaaaa attaaattta tttacagaat tgtgcggaat cattttaggc
     8221 gcaggaatct acggattagc tgtgacagga atcaatattc catctaagct ggcagatggt
     8281 ggggttactg gtattgcttt gctcttgcat aatctttttg gattcgctcc ttctgtcact
     8341 tcactgcttt ttaatttacc tttattgctt atctcattgt ttctttttgg aaaacacgct
     8401 tttctccgga cgatcgtcgg aacatttgcg ctagttttct ttttacattt atgggagagc
     8461 cttcatatac attttgctgt agatagtcta ctgatcaata gtttgatgac aggtattctt
     8521 tctggcattg gttgcggtct cgtctttcga ttcggcggaa gcacaggcgg aacagatatc
     8581 atttatcaag ccattgaaaa atattttcat attaagattg gcaaaagtct tttcttgatt
     8641 acttttggta ttttagttgt ctccttgctt tatttggatc tcacacattt tctgtataca
     8701 ttacttagct gttctattct atcttataca ttgaataaag tgaaatatct ggaaattgga
     8761 tctgttttgc cccaattgga agcaaaacag actacagaaa catttgaacc acttgaccct
     8821 cttgaacagt tagagcactg attcttattt ttcactactt tatttacaga aa
//
LOCUS       DAMEET010000094         8798 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.095, whole genome shotgun sequence.
ACCESSION   DAMEET010000094 DAMEET010000000
VERSION     DAMEET010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.095"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(360..1109)
                     /locus_tag="PB780_002212"
     CDS             complement(360..1109)
                     /locus_tag="PB780_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDE0734239.1"
                     /translation="MLKLERHQKILDLLNAEQKVIASDLSQRFNVSEDTIRRDLKDLD
                     SQGLLKRVHSGALRVGPPITNFNYRETVASEQKTALAHKALPFLKEESVIIIDGSTTN
                     LALAKEIPQDFRATIITNSPPIAIALAQHPNLEIINIGGTFYKQSMINIGVQAYKQLQ
                     NIRADLYIMGIYNIDLDVGTSVPTMQESDIKREMIRISTEVLGMITNDKFGTISNHIV
                     GPIEDLTYLISEEIPEKVRKEYNKQKIVLID"
     gene            1295..1999
                     /locus_tag="PB780_002213"
     CDS             1295..1999
                     /locus_tag="PB780_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDE0734240.1"
                     /translation="MEKKIILSPSLMCGDLANLEGSIQELESIGMDSLHIDVIDGSFS
                     PSMPLGIETIKRIREITSMNFDVHIMAKDNDNEFFIGEMLKIGVESITFHYETSLHID
                     RYIQLIKNSGTKVGIALNPATPLNVLQYILPEIDRICLMLINPGFATNKSEKQVSYAL
                     KKITDLCQLVNEKELAVDIQVDGRVSLETIPGLLDAGATNLVLGSTSLFLKGNTLAEN
                     QKLLYQAMKQSVRKNQ"
     gene            2039..2596
                     /locus_tag="PB780_002214"
     CDS             2039..2596
                     /locus_tag="PB780_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDE0734241.1"
                     /translation="MSYKAAKQNIVREITETLDRISSKDVEQLIKKIEKAERVFFVGV
                     GRVLLALEGMAKRLGHLGIQTVVVGQITEPAITDKDLLIVGSGSGESAFPLTIAKKAK
                     EFGSQVAHIGSNPDSTMKEYSDLFIRIPVQTKLALPEEISSIQPMTSLFEQSVLLLGD
                     SIALVMIEEKAIDMSKLWQYHANLE"
     gene            2604..3743
                     /locus_tag="PB780_002215"
     CDS             2604..3743
                     /locus_tag="PB780_002215"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDE0734242.1"
                     /translation="MKAVHFGAGNIGRGLIGQILHQNGFDLCFIDTNPELIHQLNQDK
                     GYTIEIIDEMNRSDFIDHVVALNSLSELEEVITATKNADILTTSVGAINLVKIAPIIA
                     QALSKRFEQKHSINIVANENVINASDVLKKEIYSLLSEKEQENFDEYAYFVNTAIDRQ
                     SLGKVLGGKMISVVEPYYEWIMDRQQLDPTTSFDMKNVNLVDDMEPYIERKLYIVNAA
                     HAAVAYLGALKGYKTIQEAIQNMEIFNLVKQFLMENIEYFVQKYHFSREELTSFVEKT
                     LNRHGNQKLSDEITRVGSSPIRKLGPNDRLIAPIVKLDSYHLPYDAGIKVIAAGYHYR
                     NLADPEAEQLRRMIVNDGLKATIKKISRLEGKLLNEVVAAYESQI"
     gene            3755..4186
                     /locus_tag="PB780_002216"
     CDS             3755..4186
                     /locus_tag="PB780_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDE0734243.1"
                     /translation="MLKEQLSGNTMFMDAIDSWQEAIRVSARPLIGKGIIEESYVQAM
                     IQNVLDNGNYIILLPLVAMPHARPEFGSKGVGMSFLCLDKPVMFPSEEPVKFFFTFSS
                     DSPKGHLDLMANLGELLSDEKMYQKLFDVKTEKALLDLIKK"
     gene            4200..5645
                     /locus_tag="PB780_002217"
     CDS             4200..5645
                     /locus_tag="PB780_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HDE0734244.1"
                     /translation="MEVANKKKRVSMRAKVQAIGGFLTNMVLPNIGAFIAWGIVTALF
                     IPTGWFPNESLNELVSPTVSYLLPLLLAYTGGRMVGGTRGAVLGTIGTIGLIIGADIP
                     MFMGAMIMGPLGGWMMKKTDDFLDGKVPAGFEMVVNNFSIGIIGFILMCLSYLVVGPI
                     IQSANQFVTSAIEALVSTGFLPILSLINEPAKVLFLNNVIDQGVYYPLGMQQTLEAGR
                     SIYFMVASNPGPGLGLLLAYSLFGKKAAKQTAPGAIIIHFFGGIHEIYFPYVLMKPLT
                     ILSMIAGGMTGIITFELFDAGLVAGPSPGSIFSYLALTPRGNFLGVMAGVVTATLVSF
                     FVTSVILKADKSTDEDRNFELSVQKSKTMKSEGRKLLKEENAREICTEKKEMNKISFA
                     CDAGMGSSAMGATIFKKKLQKAGITDVEIKNYRIEEVPEDSDLVIVHQDLEERTRKAH
                     PNMKIVTLKNYLQDPVINGLAEEIIGLKTED"
     gene            5931..7556
                     /locus_tag="PB780_002218"
     CDS             5931..7556
                     /locus_tag="PB780_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDE0734245.1"
                     /translation="MKKIYSKEIDCFRGIAVLAVIFYHLPNSLLPGGLQGVTIFFVVS
                     GFLMAKNTIRDEKNRQFSFLHFYYKRIKRIYPFLLFSLVVFIFILGISNIRLIGNIRS
                     ELPSLIFGYNNWWQLNQGVSYFDSYFNASLFKHYWSLSVELQFYLIWPFLFIVIKRMR
                     RKQVFYLIYTLIFVSILFSLFLPSTKAYYHTIAKLFPFLLGVWGYFNRITIGRFFEQN
                     TFSKIWLLLLASLCLILFPIFPYTLNELLISICFALLLASVDDMNIAKKTKIASTKLS
                     FVGKISYELYLVHFPVLIALTNLFKHHHWKYLDIAGLFTVFLCTLLMITCFRKGVQKM
                     RSPKIWTAMALMIIGVSVLVLRAPASRLTADQNQLQSLLKENSSKANAGENLLSQEKK
                     ILFIGDSVMLGAYQELKETFKKEALVDAKESRQITALPEILKEHKDLVSYQQIVIGLG
                     TNGVITDEAIEQTMSLLKDKKVYWVNIKAPTGWQDTINATLATLPNQYSNITMIDWYS
                     ESKNHPEFFYDDETHLNESGRTAYAKYIASVIQ"
     gene            8188..8541
                     /locus_tag="PB780_002219"
     CDS             8188..8541
                     /locus_tag="PB780_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734246.1"
                     /translation="MNSFKKKFVSRFLNMKNFFEKKGDYDAYNYVLNLEKQTYYTLRK
                     NDDNDFWTMLPKMLSIDSKLSLLEEFINLDIEEFCGGKELINMVESDYHTYYKESCGY
                     KVDEQCPQSIIFYIE"
BASE COUNT         3005 a         1243 c         1750 g         2800 t
ORIGIN      
        1 tttatagaca tgtgaattta tttgcagaac ctttatttgt attatacgaa tcaaattcct
       61 caatcgtcag tgaacttgct aatttaatga tagcattgct gtttactccc gcgttgagag
      121 tcataactat tttggtctgt ttagtgctat tgttttttgt taataaaaag aaataacgta
      181 gtatttcaaa agacaaagaa gtgggatgat gattgctata gtatgttata cgtggtgcta
      241 ggtaagtgag aataaacaaa aaaggaaaca cgcaataaag tttatttcaa aactttattg
      301 cgtgtttctt ttttagattt tttatatctg ttgctttagt agttaccaat agttttcgct
      361 taatcaatca aaacgatttt ttgtttattg tattctttac gaaccttttc aggaatttct
      421 tctgaaataa gataggtaag gtcttcaatt ggaccgacga tatgattact gatggtgccg
      481 aatttatcat tagttatcat cccaagtact tcagtagaaa tacgtatcat ctcacgttta
      541 atgtcggatt cttgcatagt tggcacgctc gttccaacat ctagatctat gttataaata
      601 cccataatat aaaggtcggc tcgaatgttt tgtagttgct tataagcttg taccccaata
      661 ttgatcatag attgtttata aaaagtaccg ccaatattga taatttccaa attaggatgc
      721 tgtgctaatg ctatggcaat cggcgggcta ttggtaatga ttgttgcacg aaaatcttgt
      781 gggatttctt ttgctaaagc caagttagtt gtacttccat caatgatgat gacagattct
      841 tcttttaaaa aagggagggc tttatgagct aatgcagttt tttgttcgct agctacagtt
      901 tctcggtagt taaaattggt aattggcgga ccaacacgta aagcgccgct gtgtactctt
      961 tttaagagac cttggctatc caaatctttc aaatctcgtc gaatcgtgtc ttcggataca
     1021 ttgaaacgtt gacttaaatc actagcgata actttttgtt ctgcgtttaa taaatctaga
     1081 attttttgat gacgttctaa tttcaacatg ttgcgtgctc cttcttattt attcttgttt
     1141 atcatgtcat ttatgcgttt ttatgtcaat aatttccgtt tttaatacac taaaaaggca
     1201 taaacaagca aaaacaaaat atttttatgc agttttatgt tgactagtat ttgttaagcg
     1261 gttacaatga gggggtaatt aaaggaggga atgtatggag aaaaaaatta ttttgtcgcc
     1321 ttcacttatg tgtggggatc tagcaaattt ggaaggaagc atccaagaac tagaaagtat
     1381 tgggatggat tccttgcata ttgacgtgat tgacggttct tttagtccca gtatgccttt
     1441 agggatcgaa acaatcaaaa gaattagaga aatcacttca atgaattttg atgtacacat
     1501 catggctaag gataacgata acgaattttt tattggagaa atgttgaaaa ttggcgtaga
     1561 gagcattact tttcattatg aaacaagcct ccatattgat cgatatatcc agctcatcaa
     1621 gaattctgga acaaaagtag ggatagcttt gaatccagca actccgttaa atgtattaca
     1681 atatatttta cccgagattg ataggatttg tttgatgctg attaatccag gttttgcaac
     1741 aaacaagtca gaaaaacaag tttcttacgc gctaaaaaaa attacagatc tttgccagtt
     1801 agtaaatgag aaagagttag cagtagatat ccaagtggat ggacgggtat cgttagaaac
     1861 gatcccggga ttattagatg caggcgcaac aaatctggta ttaggaagta caagtttgtt
     1921 tcttaaagga aatacattag cagaaaatca aaaactactt tatcaagcga tgaagcaatc
     1981 agttaggaaa aatcaataga tgtagtggtc ctgaataata gaaatggagg ggaaatagtt
     2041 gagttataag gcggcgaaac aaaatattgt aagagaaatt actgaaacat tagaccgtat
     2101 ttcttcaaaa gatgtggaac aattgataaa gaaaattgaa aaagcggaac gagttttttt
     2161 cgtgggggtt ggcagagtac tgttagcgct agaaggaatg gcaaaacgct tagggcatct
     2221 aggaattcag acagtagtcg taggacaaat cactgaaccg gcaattacag ataaagattt
     2281 attgattgtt ggttctggta gtggggaaag tgctttccca ctaactattg caaaaaaagc
     2341 aaaggaattt gggtcccaag ttgcccatat aggatcaaac ccagacagca caatgaaaga
     2401 atattcagat ctttttattc gtatacccgt acaaacaaaa ttagcgttac cagaggaaat
     2461 ttcttctata cagccaatga ctagcctgtt tgaacaaagt gttcttttgt taggagatag
     2521 tattgcttta gtgatgatag aagaaaaagc aattgatatg tcaaagttgt ggcaatacca
     2581 tgctaactta gagtaggtga aaaatgaaag cggtacattt tggagcaggg aacatcggca
     2641 gagggcttat tggacaaatt ctacatcaga atggattcga tctatgtttt attgatacga
     2701 atcctgaact gatccatcaa ttaaatcaag ataagggtta tactatcgaa attattgatg
     2761 aaatgaacag gagtgatttc attgatcatg ttgtggcact gaatagttta tccgagctag
     2821 aagaggtgat tacagcaaca aaaaatgcag atatcttaac aacatctgtt ggcgcaataa
     2881 atttagtaaa aattgcaccg atcattgcgc aagccttaag taagcgattt gaacaaaagc
     2941 atagcatcaa tatcgtagct aatgaaaatg tgataaatgc aagtgatgtg ttgaaaaaag
     3001 aaatttattc tttgctgtca gaaaaagaac aagagaattt tgatgaatat gcatattttg
     3061 tgaatacggc aatcgatcga caatctttag gaaaagttct tggaggaaaa atgatctctg
     3121 ttgtcgaacc ttattatgaa tggataatgg atcgacagca gttagatcca actacatctt
     3181 ttgatatgaa aaatgtaaat ttggttgatg atatggagcc ttacattgag cgaaagcttt
     3241 atatcgttaa tgctgctcat gcggcagtag cttatttagg cgcactgaaa ggatataaga
     3301 cgattcaaga agcaatccaa aatatggaga ttttcaatct agtcaagcaa tttttaatgg
     3361 agaatataga gtactttgta caaaaatatc atttttctag agaagaatta acatcttttg
     3421 tggaaaaaac attaaaccgt catgggaatc agaaactctc cgatgaaatt actcgtgtag
     3481 gaagttcccc tattcgaaaa ttaggcccta atgatcgttt aattgctccc atagtaaaac
     3541 tagattcata tcatttacct tacgatgcag gaataaaagt aattgctgcg ggatatcatt
     3601 atcggaatct tgctgatcca gaagctgaac aactacgtcg aatgattgta aatgatgggt
     3661 tgaaagcaac gattaaaaag atttctcgtt tggaaggtaa gctgctgaat gaagttgtcg
     3721 ctgcttatga gtcccagatt tagaggagga gaatatgcta aaagaacaat tgtcaggaaa
     3781 tacaatgttt atggatgcga tcgattcatg gcaagaggca atccgagtaa gtgcgcgtcc
     3841 tttgatagga aagggaataa tagaggaaag ctatgtacaa gcgatgatac aaaatgtgtt
     3901 ggataacgga aattatatta tattactgcc actagtagca atgcctcatg cgcgtccaga
     3961 atttgggagc aaaggggtcg ggatgagctt tttatgttta gataagccag tcatgttccc
     4021 aagtgaagaa ccagtaaaat tcttctttac cttttcttct gattcgccaa agggacattt
     4081 agacttaatg gccaatcttg gagaattgct gagtgatgaa aaaatgtatc aaaaattatt
     4141 cgatgtgaag acagaaaaag cattgttaga tttgataaag aagtaaagga gagaagaaaa
     4201 tggaggtagc aaacaaaaaa aaacgagtct ccatgcgagc taaagttcaa gcgattggcg
     4261 gtttcttaac aaatatggta ttgccaaata taggcgcatt tattgcatgg ggaattgtta
     4321 ctgcattatt cattccaact gggtggttcc caaatgaatc tctgaatgaa ctcgtaagtc
     4381 cgacagtcag ctatctattg ccgttactac ttgcctatac tggtggtcgg atggttggtg
     4441 gtacacgtgg agctgtttta ggtacaatcg gaacaatagg tttgattatt ggtgctgaca
     4501 taccaatgtt catgggagca atgattatgg ggccacttgg tgggtggatg atgaaaaaaa
     4561 cagatgattt tttagatggg aaagttcctg ctggttttga aatggtcgtc aataattttt
     4621 ctataggaat tattggtttc atactgatgt gcctttctta tctggtagtt ggaccaataa
     4681 tccaatcagc taatcaattt gtaacgagtg caattgaagc attagtttca actggttttt
     4741 taccgatact gtccttaatc aacgaaccgg caaaagtctt atttttaaat aatgttattg
     4801 accaaggtgt ttattatcca ttaggtatgc aacaaacatt agaggcaggc agatcaattt
     4861 atttcatggt agcatcaaat cctggccctg gcttaggact attgttagct tattctttat
     4921 ttggtaaaaa ggcagcaaaa cagacagcac caggtgcaat catcatccat tttttcggcg
     4981 gaatacatga gatttatttt ccatatgtat taatgaaacc actcactata ttatctatga
     5041 ttgctggtgg aatgacagga atcatcactt ttgaattatt tgatgcgggc ctggtagcag
     5101 gtccaagtcc aggttctatt ttttcttatt tagctctaac gcctcgggga aattttttgg
     5161 gtgtcatggc gggagtggtc acagcaacac tggtttcctt ttttgtaaca tcagtaattt
     5221 taaaagcaga taaatcaaca gatgaagaca gaaattttga actatcagtt caaaaatcga
     5281 aaacgatgaa atcagaagga agaaaattat taaaagaaga aaatgctaga gaaatatgta
     5341 ccgagaaaaa agagatgaat aaaatttcct ttgcttgtga tgcaggaatg ggaagcagcg
     5401 caatgggcgc aacaatattc aagaaaaaat tgcaaaaagc aggtattact gatgttgaga
     5461 taaaaaatta tcgaattgaa gaggtaccag aagatagtga tttggtgatc gtacatcaag
     5521 atttggagga acggactaga aaagcacatc ctaacatgaa aattgtgaca ttaaagaatt
     5581 atttgcaaga tccagtaatc aatggattag cagaagaaat cattggatta aaaacagaag
     5641 actaatttat gggaattttt gattaacctt tattaaaatt attttgccta atataaaaag
     5701 gttgtgacat ggcttgtgtc gcaacctttt tttgtcgcac aaataattaa tcggatattt
     5761 tatgttcata ttttttgtga tcaacgagaa ctgatggggc aaagatacat tttagatact
     5821 tttaactgtt aatttacaaa acaaggtgaa acgaatagaa aaatgaaaaa atcgatacgg
     5881 aagaatatgt ttgattaaaa catgcatgaa gagaaggagg ttgatgatac atgaaaaaaa
     5941 tatattcaaa ggaaatcgat tgttttagag gcattgctgt tttagcagtg atattttatc
     6001 atttgccaaa tagtttgctg ccaggcggat tgcaaggagt aacgatcttt tttgtggttt
     6061 ctggtttttt aatggctaaa aatacgataa gagatgaaaa aaacagacaa ttcagttttt
     6121 tacattttta ctataagcgt atcaaacgta tttatccatt tttactgttt tctttggtcg
     6181 tattcatttt catactaggg attagcaaca ttcgattgat aggaaacatt cgtagtgagc
     6241 tacccagttt gatttttggc tataacaatt ggtggcagct gaatcaagga gtatcttatt
     6301 ttgactctta ctttaatgct tctttattta aacattattg gtcgctttca gttgaactac
     6361 aattctattt gatatggcca tttttgttta ttgtcattaa gcgtatgaga agaaaacaag
     6421 tgttttactt aatctataca ttgatttttg tttctattct ttttagtttg tttttacctt
     6481 caactaaggc atattatcat acgatagcta aactttttcc atttttatta ggagtttggg
     6541 ggtattttaa tcgaataact attggccgtt tctttgaaca aaacactttt agtaaaatct
     6601 ggcttttatt actagctagt ctttgtctga ttctgttccc gatttttcct tatacactaa
     6661 atgaactact gatcagtatt tgttttgctc tattgttagc cagtgtcgat gatatgaaca
     6721 ttgcaaaaaa gaccaaaatc gctagtacaa aactttcttt tgtaggaaaa atcagttatg
     6781 aactttattt ggtgcatttc ccagtattga ttgctctgac aaatctattc aaacatcacc
     6841 attggaaata tttggatatc gcaggtctat tcactgtgtt tttatgtaca ttactcatga
     6901 ttacttgctt tagaaaagga gtgcaaaaga tgagatcacc caaaatttgg acagctatgg
     6961 cactaatgat aatcggagtt tctgtactgg tattacgtgc tcctgccagt cgactgaccg
     7021 ctgaccaaaa tcagttgcaa agtttattaa aagaaaactc tagtaaagca aatgcaggag
     7081 aaaatctgtt gtcacaagag aagaaaatat tatttatcgg tgattctgtt atgctggggg
     7141 cgtatcagga actaaaggag acatttaaaa aagaagcatt ggtagatgca aaagaatcaa
     7201 gacaaatcac tgcgttacca gaaatcttaa aggagcataa agatttagtt tcttaccaac
     7261 aaatagtgat cgggttagga accaatggtg tgataacaga tgaagctata gaacaaacaa
     7321 tgtctttgct gaaagataaa aaagtatact gggtaaatat aaaagcacca actggctggc
     7381 aagatacgat aaatgccaca ttagctacat tgccaaatca gtattctaat ataacgatga
     7441 tcgattggta tagtgagagc aaaaatcatc ctgaattttt ttatgatgat gaaacccatt
     7501 tgaatgaaag tggacgtaca gcttatgcaa agtatattgc ttcagttatt caataaggta
     7561 gtgattacgt gtgttaacct tactttttcc taataggtga atcaatttaa ataaaaaata
     7621 cttcatatga taaacggtgt ttaagaggag gagcttttta atattcgtgg taaaaaagag
     7681 atgggcgagg tcgttaactg agagcttctt ggaaagaaat cgctttggat ttatcctctt
     7741 tgttatacag aacatgagcg agacgacaat actaggatgt atgttctgat cgaagtttta
     7801 tggttacaag cagcaatcag cttagataaa attaaggatc gtaggaacca agtaaagaag
     7861 gctgttagta aagaaacaaa cgatcaagta tgttttactc ttaagaagat gcagtgaata
     7921 aataatagta atataatgct agaagggact gtgacaaaag acgtgtcaca gccttttttg
     7981 ttattttaat tatcgtccag tcttttttta taaatgattg cttaagcatt aaatattaaa
     8041 aaatatagag ttcaaaatta ctgcacaatt agatgaaaaa tttgaacaac aaaaagtgta
     8101 tttttatact ataatagctt ttgaatttag ttactctatt caaaagttat gctatggaca
     8161 agaaaaagaa taaatagagg tgagaaaatg aatagtttca agaaaaaatt tgtgagtaga
     8221 tttttaaata tgaaaaattt ttttgaaaaa aagggagatt atgatgcata taattatgta
     8281 ttaaatctag aaaagcaaac atattataca ctacgtaaaa acgatgataa tgacttttgg
     8341 actatgttac ctaaaatgct ttcgattgac tctaagcttt cccttttaga ggaatttatt
     8401 aatttagaca ttgaagaatt ttgcggggga aaagagttaa ttaatatggt agagagtgat
     8461 tatcatacct attataaaga aagttgtgga tacaaagtgg atgagcaatg tccacaatca
     8521 attatttttt acattgagta atatgaacta ttaagcagga aagcaactag aagctactga
     8581 tttagtattt acaaatataa actatattgc taacaattat tttctagaag gaataaaaga
     8641 actaagaatc aagacgttat cttgattctt agttctttta acatggaaaa gatggataat
     8701 gcactgttaa ttcatgatgc atcatcagaa aagttggaag gagaagatag aaatcattaa
     8761 atgatttcta cagatggtaa atgaaaaaac aaaatatt
//
LOCUS       DAMEET010000095         8634 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.096, whole genome shotgun sequence.
ACCESSION   DAMEET010000095 DAMEET010000000
VERSION     DAMEET010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.096"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..385
                     /locus_tag="PB780_002220"
     CDS             <1..385
                     /locus_tag="PB780_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734247.1"
                     /translation="QLRWNENFIRKFVKSLSLINSEQAGRQEIEIFVVSESSVKQFFY
                     RTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQNGLETEKTKIYP
                     ISLKGANIHLYHIAQELHLLKKHIS"
     gene            606..2369
                     /locus_tag="PB780_002221"
     CDS             606..2369
                     /locus_tag="PB780_002221"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDE0734248.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            2453..2911
                     /locus_tag="PB780_002222"
     CDS             2453..2911
                     /locus_tag="PB780_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDE0734249.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3020..3703
                     /locus_tag="PB780_002223"
     CDS             3020..3703
                     /locus_tag="PB780_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDE0734250.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            3850..4464
                     /locus_tag="PB780_002224"
     CDS             3850..4464
                     /locus_tag="PB780_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDE0734251.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            4480..4686
                     /locus_tag="PB780_002225"
     CDS             4480..4686
                     /locus_tag="PB780_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734252.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            5227..6357
                     /locus_tag="PB780_002226"
     CDS             5227..6357
                     /locus_tag="PB780_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDE0734253.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            6449..7204
                     /locus_tag="PB780_002227"
     CDS             6449..7204
                     /locus_tag="PB780_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDE0734254.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            7262..>8634
                     /locus_tag="PB780_002228"
     CDS             7262..>8634
                     /locus_tag="PB780_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HDE0734255.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWG"
BASE COUNT         2961 a         1427 c         1810 g         2436 t
ORIGIN      
        1 ccaactaaga tggaatgaaa atttcattcg aaaattcgtc aaaagtcttt ctttgatcaa
       61 cagtgaacaa gcggggagac aggaaataga aatttttgtt gtttcagaaa gttcagtcaa
      121 acaatttttt tatcgaaccc aatttcattt gtatgcggaa gaaggcatcg ctgaaataaa
      181 tcctatttta tttcgttcat tagaagaatt gcctgatgaa tgtctatgtg cgaaaaaaag
      241 agtggttttg tgcgatgctg ctagctatca aaatggatta gaaactgaaa agacaaagat
      301 ctatccaatc tcattaaaag gtgcaaacat ccatttgtat catattgctc aggaacttca
      361 cctattaaaa aaacatattt cttgatgaga ccttaattag gagaccttct acaaagcttt
      421 ttaattgtct gtagaaggtt tttatttgag tcctgattcg tgcgaagatc tacttatata
      481 acaagctttc tctgaaattt ttttaaaatg ctattgactt agagtcaact ctaagtagta
      541 gaatagataa agtgtaacaa atctattttc attaaaagaa gatagtacgg aggagagaat
      601 gtattatgag tacagtatta catgtattgg atgcaatcaa tagccccgca gatgtcaaaa
      661 aattatctgt tgaggaaatg aaacagttgg cagaagagat gcgccaaaaa atcttgcaaa
      721 aggacagcga tgtaggtggg cacgttggtc cgaatctagg tgtagtagaa ttaacgattg
      781 cgtttcatta tgtatttgat tcaccaaagg ataaaatcgt ttgggatgtt tcccatcagt
      841 cttatggtca taagctttta actggtcgaa aaaatggttt tgaaaaagga cattaccgtg
      901 aagtgagcgg atattccaac caacatgaaa gtaaacatga ctattttaca gtggggcaca
      961 catctacatc tatcagctta gcaacaggtt tagctaaagc gagagatctg aagaaagaat
     1021 caggaaatgt ggtagcattt atcggtgacg gctccctaag tggtggctta gcttttgaag
     1081 gattgaacaa tggtggtgtt ttacaatcca atctaatcgt attagtaaac gataatgaga
     1141 tgtctattga tgagaatcat ggcagtttat ataaaaactt agctgagctt cgtcaaacca
     1201 acggtaaaag cagcaataac ctatttaaag caatgggatt tgattatcgt tacgtggata
     1261 atggaaacga tctggaaacg gtgatccgtt tatttgaaga agtaaaagat attgatcatc
     1321 cgattgtact tcacgtccat acggaaaaag gtcgcggtta tcagccggca atcgataata
     1381 aaatgaaata tcattggcat gtaccatttg atctgtccac cggggaggca aagcaagtaa
     1441 cttctggaga aacatttaat atgatcatca tggattattt ggataagaaa atgcaaacaa
     1501 gtacagctcc tattgccgca gtcaatgcag cgataccagg cgcctttgga ctgaaaacat
     1561 tccaagaaaa acatccagat cattattttg acgtggggat cgctgaacag cactctatca
     1621 ctttcgcttc tgctttggct tctcagggag tacgtccagt cgtcttccaa tcaagtactt
     1681 tcttgcaacg ggcatatgat caattatcac atgatttagc gatcaacgag aatccagtga
     1741 ttattttagt cggtggcgga acgatctcag ctagtgaccg aacacaccaa ggaacatttg
     1801 acattcctta cttatcaagt atcccgaaca tcaaatatct tgctccagca acaaaagagg
     1861 atatgatctc tatgctagat tgggcgttcg aacaaacagc gcagcctgta gcaatccgaa
     1921 ttcctacaca tggtgtagaa catggtacag ctatcgcaaa tgattatagt attccagcct
     1981 atcaaacagt aaataatgga ggaaaagtag caattttagc tttaggcggt tttcttgaat
     2041 taggaaaaga aacagtaaaa gcttttagaa aaaaaggaat cgaagcaacg ttgatcaatc
     2101 caggaacgat cacagaaaat gacgtagaaa cattagataa attaaaagaa acacacgaat
     2161 tagtcatcac attagaagat ggtagtttgt caggaggatt tggcgaaaat atcagtcgtt
     2221 attatggtga ttcttcaatg aacgtattaa attttggtgc agagaaagaa ttcacggata
     2281 gcgttcctgt agggcagctt tatgaacgtt atcatttaac accaaaacaa atcgttgaag
     2341 atagtctgcg tgtcttgaat attcaataat tcattatgct caccatccag ataattgttt
     2401 atactagtat ctggatggtt ttttcattat aaaaagcaaa ggggtggatg ttatgtatca
     2461 gcaagtagaa gaatatgtca aaaagttcca agaaaaatat cctagttatc aatcagaaaa
     2521 attaggtttc ttttcatttg gggatacaga aaaaatggca gacgagttga gcgaactagt
     2581 tgtaaaaaag ataaagactg gtacatcaag tggttacgat ctgtataaac aagagaatga
     2641 gcctttacca aaagttggtc aattagacgt ggtcctgaat ggaaaaaatg aaccagtttg
     2701 tatcatcaaa aatataaatg tgaccatctt gccttttagt gaagtcacga aagagcatgc
     2761 ttggaaagaa ggagaagggg atcggacatt agcttattgg cggaaagtcc atgctgagtt
     2821 ttttaaacat gcttataaag aagcgttgaa catagaattt acagaagaga agctggtcgt
     2881 atacgaagaa tttgaagtaa ttttctttta gtaagaaagg tctgctgaaa gaaatagctc
     2941 attattgtct cctgaataga aaagggttac atttttatat gaagctgtat ttttatataa
     3001 cattactgga ggagatattg tggatacact ggaacaaatc aaaggcggag tcgtcgtttc
     3061 ttgtcaagct ttggaaaacg agccgctaca cagttctttt attatgggga gaatggcttt
     3121 ggctgcaaaa gaaggcggag cagttggtat tcgagcaaat acgacagaag atatcaatga
     3181 aatcaagcga atcgttgatt taccggtaat agggattatc aaaagagatt acgatgggtc
     3241 agaagtgttt attactgcga cagaaaaaga agtggaagaa cttttggaga cgcaagcaga
     3301 aatcattgca ctggatgcaa cttctcgtaa acgtccgaca gaaaaaacaa ctgctgaact
     3361 tgtccaaatg attcacgaac acggtcggtt agctatggca gatatttcga cttttgagga
     3421 agcgcttcaa gcgcaagaag atggttttga tcttttgtct acgactttag caggctatac
     3481 agaatattca cgaaagacgc agacacctga ttttgattta ttgaatcaaa tcatagaaca
     3541 agtcgatatc ccagtaatta tggaaggaca tactgactct cctgaacaag tagagaaagc
     3601 atatgaatta ggtgcttttt cagtcgtagt tggatctatt atcacccgtc cgcaattgat
     3661 cactaaaaga tatgtcgaag ccgcaagtaa agcaaataac taaaaaacac gcacggcaca
     3721 gattcttgac aaaaaagaaa aattgataga tagtttaaag cagattctaa gaagatgaaa
     3781 aaagcattac ttttcatttt gtcttcaaga tctgcttttt aacgaatttt atagaaagaa
     3841 ggcgaaacca tgtttgaacg gagtccttca gaattgacag aacgtgaaaa ttacaagttg
     3901 ctgattggtt cagtcatccc tagaccagta gcagtagtaa cgactaaatc agcaaaagat
     3961 atagtgaata tcgcaccctt cagttacttc aatatcgtca gttcgaatcg acccatctta
     4021 tccttggcga tccagagaaa acaagatgca gtaaaagaca cagcaaagaa tattcttgag
     4081 acaagagaag ctgttatcca tatccttgat gaggacaacg tagaagaagt aaataaaaca
     4141 gcagccaatc tgcctaatga ggaaagcgaa ctgtacctga ctgacttgac tttaactgat
     4201 tcagtaatca tttcagtacc agcattgaca gaagcaaaag ttcgttttga aacatcctta
     4261 tatgaacatg tcgagatacg gaataaaaat aaagtaacag ctgatttatt tttgctagaa
     4321 gtacagaaat atcatttaaa tgaaacagtc tatgatgata agacaggaag gatcgatcca
     4381 agaaaattga atgctgtaag ccgtttggca ggaaatgatt atgcagcaat aggagaaatt
     4441 ttcacgattg cacgtcccga ataaaaagga gcactagtta tgttaaaaat aagctataaa
     4501 ccatcgacag attcaaaaga aatgaaaaaa gaatatgaaa cagtgaatga ttttctgcaa
     4561 gggcagtact tagaagttcc gccattgcaa gaccattttg tcgttactac tgttacttta
     4621 gatggtaagg aaatagaaat gccagatcaa acgattagcg gattgtttaa ttactttaac
     4681 aaataaatat ttattccgaa aagtgaaaaa acatataaaa aaacacataa aactaaaaaa
     4741 ttatcaaaga atcctggtgc aactgaaaga atttgcaacg ggattctttc aacttatttg
     4801 ttattttttt ctaaaaaata ttaaagattg ttaaatttca ctatttcatt tcaaattcca
     4861 ttgcctaatc attaggaaaa gtctatcatg aactcgtaaa tagaatatag taataacctt
     4921 gttaggtgag gctcctatac aaacataggc tgttgcccag aaacgtcgaa agtcgccaat
     4981 gggtaaaaca gaaactgttg gattaaggct ttttccaata gctaagaatg ttttctttac
     5041 gttgtatagt gctaaaactc ggacgatgag atgatgctag gtcagaattc ttgttcgaaa
     5101 aggtttctga ccttttcttt atatttttgt gactgactta tttgatgaga caaactcgct
     5161 cctcacctga caagtggatt gctgtgtgag gaggagatgc ggtgaaaaaa gatcgattgg
     5221 tggattttgg gtccttattt gactttgtcc atgatcggtc ttttagaagt atacagtgcc
     5281 agttcttatc gtttattgca agccgatgaa aatacaaaaa gtctattatt gcgtcaacta
     5341 attttcatat ttttgagttg gagcgtgatc ttcttagctc gttcagtcaa actacactat
     5401 ttacttcacc ctaaaatagc aggatacggt ttagccttat cgattttctt tttagtatta
     5461 gtaagaatag ggatattcgg tgtcactgtc aacggcgcac aacgttggat ctctctgttt
     5521 ggcattcaat tccagccttc tgaactggca aatctttttt tgatttttta tttaagctgg
     5581 ttttttcgtg acggaaatag tagcccaaaa gatctaaaaa aaccattcct gattacagta
     5641 ggtataactt ttctgatttt atttcagcca aagattgctg gagcattgat gatcctttcg
     5701 attgcgtggg tcatattttg ggcagcggcg gttccattta aaaaagggat ctatctaatc
     5761 gttacttttt ctgcattgct gattggagca gcaggcgggg tattatattt aggaaataaa
     5821 ggttggcttc cacagatgtt taatcatgct tatgaaagga tcgcgacttt aagagattct
     5881 tttatcgata gtcatggtgc gggctatcaa atgacccatt cgttttatgc actatataat
     5941 ggtggcatat ttgggcgggg attaggaaat agtatcacaa aaaaagggta tcttccagag
     6001 acagaaacag attttatttt ttcgattatt acagaagagc ttgggctaat cggtgcattg
     6061 tgtgtgcttt tcttattgtt ctcattatgc atgagaatct tttgtttaag cagtcgctgt
     6121 aaaaatcagc aagctggatt gtttctttta ggattcggaa ctttgctatt cgttcagacg
     6181 ataatgaacg tgggaagtat cgcggggctg atgccaatga ctggtgtgcc attgccattt
     6241 gttagctacg gaggaacaag ttatctgatc ctttctttag gaatcggaat cacgttgaat
     6301 atttcttcaa agatacaggc cgaagagttg gcgctctatc gacctgaaaa acaataaaga
     6361 gaaactgaga aactctcagt ttgaattatc ataaagtaca atcttttatt tttcaagcga
     6421 gaggttcttt tagaaagagg aattcgcaat gaaagtatct caaaaaatca tattatctat
     6481 tttgttactg cttacattag tagttggcta tttttcgatt gagtttatcc atggattttc
     6541 gtctgcaaaa cagagttcaa ctgtgaaaaa ggtagagccg aaaagtatcc ctaccacact
     6601 aaatgtggct ttgattggtt cagatgctcg ttcgaaagac gaaaacggtc gctcggattc
     6661 acttctggtt gcacaataca accagaaaac acaacaagca aaactgatct ctatcatgag
     6721 agattcatat gtcgatattc caggttacgg aatgaataag ataaatgcag cttactctta
     6781 tgggggagtt gatttattaa atcaaacatt aaaggaaaat ttcaaatttg aagctcccta
     6841 ttatgcaagt atcacatttc aagattttat cgattgcgtt aatgaactgt ttcctggtgg
     6901 agtcaagatc gatgcagaaa aatctctaga tttagatggc gtatatataa acaaaggaga
     6961 gcaagtgatg gatggtaata ctttactcca gtatgctcga tttcgggaag acaaagaagg
     7021 agacttcggg aggatcagaa ggcagcagca agtgatcaaa gctatctctc agcagctgaa
     7081 agacgtaact tcaatattca agctacccaa agcggttggg aaattacttg gtagtatcca
     7141 aacgaacctt ccagaaagtg tgcttctgga ttgtggtatg gatttcctca aagacatatg
     7201 ttagaataaa caggtataaa tagtgaaata aaggaatgac aagcaagaga aggaggaaaa
     7261 aatgaaaaga agtgacaagc acggcaaaaa tcgaacaggc gcttatattg ccggcgcagt
     7321 gatcttaata gcagctgcgg gtggcggtta tttttactac cagcactatc aagaaaccca
     7381 agcagtagaa gctggagaaa agacagttga gcaatttgtc caagctttga acaagggaga
     7441 ttataacaaa gctgcagaaa tgacatcaaa aaaggcggca aataaaagtg cattatctga
     7501 aaaagagatc ttagataaat accagaatat atacggtgct gctgatgtca aaggacttca
     7561 gatatcgaat ctaaaagtag ataaaaaaga cgattctact tacagttttt catataaagc
     7621 aaagatgaat acctcattag gtgaattgaa agatctttct tataaaggaa cattagacag
     7681 aaatgatggt caaaccacga tcaactggca acccaacttg gtttttccag aaatggaagg
     7741 aaatgacaaa gtaagcctga ccacacaaga agcagcaaga gggaatatta tagatcgaaa
     7801 tggtgaacca ttagcaacaa caggcaaact aaaacaatta ggagtcgttc caagtaaact
     7861 tggagatgga ggcgaaaaaa cagccaatat caaagcgatt gcttcctcct tcgacttaac
     7921 agaagatgct atcaatcagg cgatttcaca aagctgggta caacccgatt actttgtccc
     7981 attgaaaatc attgatggag caacgccaga acttccagct ggagctacca ttcaagaagt
     8041 agacggcaga tattatcctt tgggtgaagc agctgctcag ctgattggtt acgtgggaga
     8101 tatcacagca gaagatattg ataagaatcc agaattaagc agtaatggaa aaatcggtcg
     8161 ctcaggtttg gagatggctt ttgataagga tcttcgtggg acaacaggtg ggaaattaag
     8221 cattacagat gcagacggtg tagagaaaaa agttctgatc gaacatgaag ttcaaaatgg
     8281 caaagatatc aaattgacaa tcgatgcgaa ggcacaaaaa acagcttttg acagtctagg
     8341 aggaaaagct ggatcaactg ttgcgacaac gccaaaaacc ggtgatcttc ttgcgcttgc
     8401 tagctctcca agctatgatc caaacaaaat gacaaacggg atctcacaag aagattacaa
     8461 agcttatgaa gaaaatcctg aacatccatt catcagccga tttgcgacag gttatgctcc
     8521 tggatctacg tttaaaatga ttacagcagc aatcggtctc gacaacggca ctatcgatcc
     8581 aaatgaagtg ttgacgatca acgggcttaa atggcaaaaa gacagttctt gggg
//
LOCUS       DAMEET010000096         8444 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.097, whole genome shotgun sequence.
ACCESSION   DAMEET010000096 DAMEET010000000
VERSION     DAMEET010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.097"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            124..2733
                     /gene="clpB"
                     /locus_tag="PB780_002229"
     CDS             124..2733
                     /gene="clpB"
                     /locus_tag="PB780_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDE0734256.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2884..3570)
                     /locus_tag="PB780_002230"
     CDS             complement(2884..3570)
                     /locus_tag="PB780_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734257.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3548..4042)
                     /locus_tag="PB780_002231"
     CDS             complement(3548..4042)
                     /locus_tag="PB780_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HDE0734258.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4062..5282)
                     /locus_tag="PB780_002232"
     CDS             complement(4062..5282)
                     /locus_tag="PB780_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDE0734259.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5429..7264
                     /gene="lepA"
                     /locus_tag="PB780_002233"
     CDS             5429..7264
                     /gene="lepA"
                     /locus_tag="PB780_002233"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDE0734260.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(7358..>8444)
                     /locus_tag="PB780_002234"
     CDS             complement(7358..>8444)
                     /locus_tag="PB780_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDE0734261.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         2717 a         1615 c         1698 g         2414 t
ORIGIN      
        1 tcgagaaaaa aagtcaaaaa actatgtgta tattttgacc aaaattgacc aaagcgatat
       61 aatagttttt gtaaagatca aagaaataag tgatcttacc tttaattagg agggtatacc
      121 tgtatgaata ttgagaaaat gactacgacg cttcaagaag cgattgctga agctcaacag
      181 attgcggtca ctcgaaaaca tcaagatatc gacattgccc atgtatggaa aatcttttta
      241 caatctaatc attttggtcg aaatttctat acggatgccg gattggatgt cgaatctttt
      301 gaacatgaaa ttgatcgtct actggatgag tatccagttg tttctggtgg aaacgtccaa
      361 tatggacaaa acctcagcca gaatttcttt cgattattga atgaagcaga ccaaatacgt
      421 gaaagcttcg gcgacgagtt tctttcaacg gaagtcgtta ttttagcgtt gatgaaatta
      481 aaaaattatc cgttaactgt ttatttgaat aaaaacggat tatctgaaaa agaattgcgt
      541 aaaaacatcg aagagatgag aggaggagaa agagtgacct cacaaaacca agaagaacaa
      601 tacaaagcat tagaaaaata cggcgttgat ttagtacagc aagtcaaaag cggaaaaatg
      661 gatccaatca tcggtcgtga tgaagaaatc cgtgatgtca tccgtattct ttcacgaaaa
      721 acaaaaaata atcctgttct gataggagaa cccggtgtag ggaaaacggc aatcgttgaa
      781 gggcttgctc aacgtatcgt acgcaaagat gtgcctgaaa atttaaaaga taaaacgatt
      841 ttttcattag atatgggtgc attgatcgct ggagcaaaat tccgtggtga atttgaagaa
      901 agactaaaag cagtcttgaa agaagtcaaa aaaagtgatg gacgtatcct cttgttcatc
      961 gatgaaatcc acaatatcgt aggggcagga aaaacagaag gaagtatgga tgccggaaat
     1021 ctgttgaaac caatgcttgc tcgcggtgaa ttacatttga tcggtgccac aactttagat
     1081 gagtaccgtc aatatatgga aaaagacaaa gcgttggaac gtcgctttca aaaggtatta
     1141 gtcaaagaac caacagtgga agatacgatc agtattctgc gtggattaaa agaacgtttt
     1201 gagatccacc atggtgttaa catccatgac aacgcattag tagcagcagc tactttatct
     1261 gaccgttata ttactgatcg gttcttaccg gacaaagcaa tcgatttgat cgatgaagcc
     1321 agtgcaacga ttcgggtaga aatgaactct atgccaacgg aattagatca agttactaga
     1381 cgtttgatgc agttggaaat cgaagaagct gcactgaaaa aagaatcaga tgatgcaagt
     1441 aaaaaacgct tgaaaaattt gcaagaagaa ttagctgaac ttcgtgaaga agcaaatgcg
     1501 atgaagatgc agtgggaaac agaaaaagaa gaagtcaatt ctgtttcagc aaaacgtgct
     1561 gaaatcgata aagcaaaaca cgaattagaa gatgcagaaa ataactatga tttagaacgc
     1621 gctgctgtat tgcgtcatgg tacgatacct caattagaaa aagaattgaa ggaattagaa
     1681 gctaaagcaa aagacagcga gattaagatg gttcaagagt ccgttaccga aaatgagatt
     1741 gctcaagtcg ttggcagatt aacgggtatc cccgtaacga aacttgtaga aggtgaacga
     1801 gaaaaactaa ttaaattgaa tgaaacactt cataaacgag tgattggtca agatgaagca
     1861 gttgatgcgg tcagtgacgc ggtaattcgt tcacgggctg gattgcaaga cccaaatcgt
     1921 cctctaggtt ctttcctttt cctaggacca actggtgtcg gaaaaaccga attagcgaaa
     1981 gcgttagctg agaacttgtt tgactcagaa gatcatatgg tacggatcga tatgagtgaa
     2041 tatatggaaa aacatgctgt gtctcgtcta gtcggagctc ctccaggcta tgtaggttac
     2101 gaagaaggtg gtcaattaac ggaagctgtc agacgaaatc cttatacaat cgtcttgttg
     2161 gatgaaatcg aaaaagcgca tcccgatgtc ttcaatatct tgttacaagt cctagatgac
     2221 ggacgcttga ctgattcaaa agggcgagta gtagatttca aaaatacggt tctgatcatg
     2281 accagcaata tcggttcaca gttactgctg gaaggcgtga ctgctgatgg aacgattcca
     2341 gaagcagtgg ctgaacaagt caacacgcta ttacgtggaa acttcaaacc agaattcttg
     2401 aaccgaatcg acgacacgat tttatttaca ccattgagcc tagacaacgt gaaaggaatc
     2461 gttgacaaaa tggtggcgca attggcacaa cgtctggaac atcaagaaat cctgctgaca
     2521 atcagtgatg aagccaaaac gtggattgct gaaaatgctt atgaaccagc atatggtgca
     2581 agaccattga aacgatttat cactaaggaa gtagaaacac cactagctaa agaaatcgtt
     2641 gcaggacatg ttatgcctaa atcaaaagta acgattactt tacttgatgg acaacttcat
     2701 ttcaaaacag aagagctaga agaaatcgtc taatcttcca ttgagactat aaaagaaaaa
     2761 aggggtctga cacaactagt gtcagacctc cttttttcaa ataaacagtg ttccagaagt
     2821 agtttcttcg aaaaataagc cgaaatttct tgaagctgcc cgcttctgtc acaacctctt
     2881 ttattaggta tttttcactt tttctaattt ataactttca aagtaacgct catctttttt
     2941 actttgagac catgcaaaca atcccattaa ggcagcacct atcgtatttg tcagcaagtc
     3001 gcccatcgta tccatcaatg ctgctcgtcc gacaaacagt gtgccatcag aagcagcaaa
     3061 acgttgaaga ttcatgccta agaactggtc gcataagaat tcccagaact cccaaaacac
     3121 gccgcaaagt ccagcaaacg caaagccgaa taagaggaac aaccaaggac tggtttttga
     3181 tatctcagca tctttcatca agtaaccaat caatccatag ccaagagctg tcaggaccat
     3241 tggactcacc gcatgtaaga ttttgtccca aaaagagatg atactgatca aatgcatccc
     3301 tgtgcctaaa aagacagaga tgaacaagaa aaaccagtaa aacaaaacga ttgctgctgg
     3361 tattttaagt ttgaaaactt tcaaaatgac ttgtggcatg aaaatcaatg caatccctaa
     3421 taaagattcc acgataaatg tgattccttg agaagtcgaa tatttttgtg tgataaattc
     3481 ataaaaatta tagcagacaa cgaatacacc aaaagcaagt aaagctattt gcaccttttt
     3541 gatttcttta atcactgaaa gtccctccat ctaaaaatct ttttacttct gaaagcaact
     3601 caatttctga agaaaccact tttccgttca atttgatcaa acctattgta tataagttca
     3661 ggtagtgaaa ttgattttca gctgtttctt gtagtgcagc gatttttttc gggtgggttc
     3721 cgccttgctg tcgagtatca gtatataagc cgatcactgg gatgcctttc gcataagcca
     3781 caccgatttc cgatgctacc ccggcatcga tcgtcagccc atctagtaaa gcaatcatta
     3841 aatcactagc caacactttt tctgtgtcag caagagcaat cattcgactg tctgcataag
     3901 cagttttgtc attgattgct tcattttctt gaggaagata gatcgaaagc tcagaagaga
     3961 gctctctgat ttctttaact aactgttggt tatataacaa atcactttta gcaaacattg
     4021 gtgcggcaaa ataaattttc atctaaacat catccttttt atcaaaaata atcgctaaag
     4081 taagcttcca tgtttggaat gatacgctct agtgacttca gtgtttcttc atctgtttct
     4141 aaccgttcga ttcttgccaa ataagaagca cggcgataat tcatcatcaa gcaagtcaat
     4201 gtttggatat ccagtcgaac ggattttcct ataggttcat ttaagacacg gacttgtccc
     4261 tgttcatccc atgttaaagc gaagacacca ttattccaag gagcgacagg atcttcaatg
     4321 atgaaatgaa aagcatcagc tgttccaaca aacggaaacc gttgaaggaa ttcttttaca
     4381 tcaacgatcc gagccataaa atatggttcg atctgctctt tgatctgact atcttcaatc
     4441 aaaaatgcta aaggctcatt tttataaata tctccatgta cccagtagat catggaaaaa
     4501 tgtgcggaaa taaagttcca taagccattt cgagcttctt gatcaagata gaacatttct
     4561 tttacgtgaa atatttcttc agctacccag tagaacagaa cacctcttgg ttcatggttg
     4621 ctatcgtagt agacagcggc tgtgcgctca tcttcatttt cgaaacgcca atactcttcc
     4681 caatgaaaat cactgcggaa taaagcacca tggttttgtt gggcaaattt gtcataaacg
     4741 gaaaatacgt ctggatgatc aactggtaat cgctcaacca taccacttac tgggatttgt
     4801 ttaggtagct gtgtatctct gattttaaag gaaagtttat cagacatgat ttcccatcct
     4861 ttacgccgat agtaggggat actgtaagga tacaaataag agatccattg cttattcttg
     4921 cgcatattat ccaatgcaac gataattaga tcttgcatca atccatggtt agcatattct
     4981 ggataagttc caactccagt cacaccaccc attttataac gtgcaccgtg aatattgact
     5041 tcacaaggat agatagcgat ttgagagatc agtttatttt cgtggaacca tccgaacact
     5101 ttcgatactt ccaaaatcgg ttttttggaa cggataaaag ctcgtttatt ctcaaacccg
     5161 ctttcctcga tgtctgcttc agtcacttgg aacacatagg aaagcaattc attgaattga
     5221 tcgatatgtt cttgagtgac cggttttaat tcaagatttt ctctaaaatc tttttgatcc
     5281 attttttcat ctccatttat tagcttgata acagtatagc aaaattctac aaaaaaaact
     5341 tctatagctg actaattaaa aactctttaa attactgctt ttcgttggta taatggaaag
     5401 tgctgattat atgaaaagag gagacgctat ggatataaag aaaatgaaag aacgccaaga
     5461 gcagattcgt aatttttcga tcatcgctca tatcgaccat gggaaatcta cattggccga
     5521 tcgtatttta gaaatgacgc acactgtgac gtctcgcgaa atgcaagatc agctgttaga
     5581 ctcgatggat ttggaacgtg aacgtggaat cacgattaaa ttaaatgctg tagaactcaa
     5641 ttacacagcg aaaaatggag aaacatatat ctttcacttg attgacaccc cagggcacgt
     5701 cgacttcacc tatgaagtct ctcgaagcct tgctgcctgt gagggtgccg ttttagtggt
     5761 cgatgcagca caaggaatcg aagcacaaac attggccaat gtttatttag cgttagacaa
     5821 tgatttggag atcctgccag tcatcaataa aatcgaccta cccgcagcag atcccgaacg
     5881 tgtccgtcaa gaaatcgaag acgtaatcgg aatcgatgct agtgaagctg tattagccag
     5941 tgccaaatca gggattggga ttgaagatat tctcgagcag atcgtggaat atgttcctgc
     6001 accaagtgga gacttagatg caccgctaaa agctttgatc tttgactcta tttatgatag
     6061 ttaccgcggt gttgttctaa atgtccggat cacggacggt atggtcaaac ccggagacaa
     6121 aatcaaactg atgagtaatg gcaaaacatt tgatgttaca gaggtcgggg tcttctcgcc
     6181 aaaagcagtt gctagagact ttttgatggt cggtgatgtc ggttatatca cagcaagtat
     6241 caaaactgtc caagatacac gagtagggga tacagtaacg ttagcagaca atcctgccgc
     6301 tgaagcttta ccaggttacc gaaaaatgaa tccgatggtt tattgtggtc tttatccaat
     6361 tgatacttct cgttacaacg atttgcggga agctttagaa aaactgcaat tgaatgatgc
     6421 tgctttacaa ttcgaaccag aaacctctca agcattaggt tttgggttcc gctgtggatt
     6481 cctcggactt cttcatatgg atgtcgtcca agaacgcttg gaacgggaat tcaatctaga
     6541 attgatcacg actgcaccat ccgttattta ccatgttaat aaaacggatg gtacgactgc
     6601 tacagtagat aacccggcag attttcctga acctgtaacg attcaggatg tagaagaacc
     6661 attcgtcaaa gcacagatca tggtcccaaa tgattttgtc ggtgcagtaa tggaactttc
     6721 tcaacgaaaa cggggagaat tcattacgat ggattattta gatgattacc gagtaaatgt
     6781 cgtctataat attccgcttt ctgaaattgt ctttgacttt ttcgacaaat tgaaatcaag
     6841 cacgaaaggc tatgcctcat tggattatga aatgtctggt tatcaaaaaa gcaagttagt
     6901 gaagatggac atcttgctga atggagaaaa agtcgatgca ttaagcttta tcgttcaccg
     6961 agactttgcc tatgaacgtg gaaaagcaat cgttgaaaaa ctgaaaaaac taatcccacg
     7021 ccaacaattt gaggtgccaa tccaagcagc tattggacaa aaaatcgttg cccgttcaga
     7081 catcaaagcc ttgcgtaaaa acgtcttggc taaatgttat ggtggagatg tttctcgtaa
     7141 acgtaaactc ttagagaaac aaaaagaagg gaagaagcgg atgaaacaaa tcggctccgt
     7201 cgaagttccg caagaagcct tcatggccgt tctgaaaatg gacgaggacg aaccgaagaa
     7261 atagcaaggg tttgtttgaa aacagtgtaa aaaataaacc atgtttacat ggatttgcca
     7321 ccggtttgcc accggtggtt tttttcggtg gcaaaaatca agaattcttt ctaagatttt
     7381 ccatgatatc gaccgtttca tttctcatct tatctgttac atgcgagtaa gtatccatcg
     7441 taattgaaat tctgctgtgg cccaatcgtt cagagatttc tttcattttt gcgccatttt
     7501 cgagaagaag tgtagcatga gtatgtctga gagaatggaa gttaaaagag agggagagtg
     7561 cattcgatat cctcctagta ttccatttca ctacacttgg cgtaactagc tcaccgtctt
     7621 ccttcgtaca tactgcattt gaatcaatgt agagctttcc atacttcatt cgattttcta
     7681 attgttgttt cttatgtttt ttcagaattg ctagcaaggt ttgtccaata aaaatcgttc
     7741 gattggaact gcttgtcttt ggcgtaccat atacccatgc gccgtcattc tttaccattt
     7801 gtttctctac agtaattgtt ccatttgaaa aatcgacatc atcccacgtc agaccacaaa
     7861 cttcgccaac gcgcattccc gtataaaatc caatattcaa aggaatatag aaaggatgtc
     7921 cttcaggagt aatttctagc atatgatcaa agtcctcaag agaaatgatt tttagatctt
     7981 ttttagtcgt tggtcgtgct tcatattttg gtatttttac atacagcata ggattttgct
     8041 tgattaatcc ccaaggataa accgccatat tcagcgcatt cttaaggaca gagtgagtaa
     8101 tagtcattgt tttcttcgag taaccctttt taaattcagc attgatgaaa ttttgtaaaa
     8161 gagcagggga gagatccgta agttttttct ttcctaaata accgtttata tgatttttga
     8221 tggtaaatcg gtagttttca taggtattgt attttagatt tagtttaacg tattcctcca
     8281 tccaaaaatc aaggtattgt tttactcgag tatccgtacc taaaaagtat tgtcctgttt
     8341 cgtcaatatc tgataaaact tttcgtaaag cagcttcggc ctctggtcgg gtgtctccgc
     8401 caactttctc cacttttttt cttgagccat catcattgat atct
//
LOCUS       DAMEET010000097         8374 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.098, whole genome shotgun sequence.
ACCESSION   DAMEET010000097 DAMEET010000000
VERSION     DAMEET010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.098"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            17..1171
                     /locus_tag="PB780_002235"
     CDS             17..1171
                     /locus_tag="PB780_002235"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HDE0734262.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1193..1945)
                     /locus_tag="PB780_002236"
     CDS             complement(1193..1945)
                     /locus_tag="PB780_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDE0734263.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2309..4975)
                     /locus_tag="PB780_002237"
     CDS             complement(2309..4975)
                     /locus_tag="PB780_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDE0734264.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNE
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5317..6768
                     /gene="cls"
                     /locus_tag="PB780_002238"
     CDS             5317..6768
                     /gene="cls"
                     /locus_tag="PB780_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDE0734265.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQKALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(6973..7788)
                     /gene="thiD"
                     /locus_tag="PB780_002239"
     CDS             complement(6973..7788)
                     /gene="thiD"
                     /locus_tag="PB780_002239"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDE0734266.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKDAQQETVNDYVLLESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            8082..8357
                     /locus_tag="PB780_002240"
     CDS             8082..8357
                     /locus_tag="PB780_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734267.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT         2484 a         1726 c         1488 g         2676 t
ORIGIN      
        1 gtaaagaggt gttgaaatga aaatagggat tgatcgtctt tcctttttta ttcctaattt
       61 atatttagat atgacagagt tggcagaaag ccgtggggat gatcctgcaa aataccatat
      121 agggattggt caagaccaaa tggcagtcaa tcgtgcaaat gaagacatca ttacactagg
      181 agcaaatgct gccagcaaaa tcgtaacaga aaaagaccgt gagctaatcg acatggtcat
      241 cgtcggaaca gaatccggga ttgatcattc aaaagcgagt gcggtaatta tccaccattt
      301 actgaaaatc caatcttttg ctcgttcttt tgaagtaaaa gaggcttgct acggtggcac
      361 cgcagctttg cacatggcga aagaatatgt caaaaatcat ccagaacgaa aagtactagt
      421 catagcaagt gatattgctc gttacggctt ggcaagcggt ggtgaagtga cgcaaggtgt
      481 cggtgctgtt gcgatgatga tcactcaaaa cccgcgtatt ttatcgattg aagacgacag
      541 cgtatttctg acagaagata tctatgattt ctggcgtcca gattatagcg aatttcctgt
      601 tgttgatggt cctttatcta attctacgta tatcgaatca ttccaaaaag tttggaatcg
      661 acataaagaa ttgtcgggtc gaggactcga agattatcag gcgattgctt tccatattcc
      721 gtatactaag atgggaaaaa aggcattgca aagcgtatta gaccaaacag acgaagaaaa
      781 tcaggaacgt cttatggctc gctatgaaga aagcatccgt tacagccgac gaatcggtaa
      841 tctttacact ggttcattat acctggggct aacttctcta ctggaaaatt cgaaatcact
      901 acagccagga gatcgcatcg gtctattcag ctacggttct ggtgcagtaa gtgaattttt
      961 tacaggttat ctggaagaaa actatcaaga atatctcttc gcacaatctc atcaagagat
     1021 gctagattct cgaacacgga tcactgttga cgaatatgaa acgattttca gtgagacgct
     1081 tcctgaacac ggagaatgtg cagaatatac ttcagatgta cctttttcga ttacaaagat
     1141 cgaaaatgat attcgttact acaaaatata ataaaatcat aaaaagaacc cttcacgagt
     1201 cgtgaaaggt tcttttttta tctgcggacc aaaggagcag tctataggtc agcttagtcg
     1261 tactaaggat ttctcgtttt tcactgaata gtttgcgaat gtacctggtc acaacaggat
     1321 cttttgcgta cgcgtcatag tatagcttca tcaattcttc agtaattgat tcctgtgtaa
     1381 aataagtaaa atcttttttc ttcactttga atccttcatc aattaagtat gtttcaaatt
     1441 gatccattac aactggcgat gtattaggat cttcttctat cattcctagt tgcttttcga
     1501 taggataaaa aacatcatcg attgattcca ctaccctgat tatcacgcac catttgcttg
     1561 actttttcat caatttttct aattgaataa gatgtaccaa tgctggattt gctgctgtaa
     1621 aaaccatttc atatgtctca tttgattgga taaattcttc taacgactgt ttgataaagg
     1681 aaacatttgg caactcattt ttttctgctt ctttttttgc attttctagc attcctgaag
     1741 aaaaatcgat caaatcatag cgattaattt cttttgctaa ggaaagaaat ctgccagatc
     1801 ctccagcaac gtctgctgct gtttcagcag gcaggacttt ctccgttttc agataatttt
     1861 ttacatcttc agcaatagtt acttgcgatt cttgctggat ttcataatat tcttcagcaa
     1921 attcatccca ttctttttca ttcatttgat ttcctctaat tttttgttat atgttttcac
     1981 ttttcggaat aaattattct cctcttttat ctattctatc tcttttgatg aataataaaa
     2041 tgattggctt tccgtctatc ataaataaaa aaagggactg tgaccaaagt cgtgtcaaag
     2101 tccctctttc cgaataaaca gtgtccaatc agatgaccct cagtattagc tcaaaatgct
     2161 gattttacaa cttcttcctt aaacggtgtt ctcctgccaa ctcttcggtc acaagtcaca
     2221 atattcaaca aacatgagaa gctgtttgtt gaaattgcac cttgtgactc agaattaaac
     2281 ggcaggctca caacctcgtt gattcgtttt atttatttcc ttgtactcgt acaatgatca
     2341 atttagtgat ttccatcaaa acaattgtca caagtgcgat tcccatacat gtaaagactt
     2401 ctgtcaatcc aaatgctgat gggatagaga agacttcttt cagtcctggg atcattgtta
     2461 agcagtaaag cgcgctacat acaactacag ctagtaagac acttctgtta gagaagaacc
     2521 ctgcttggat agctgtttgt gtgtttgaac gtgctgggaa tgtttgcaat gtccgacacc
     2581 aaatcaatgt tgaaaatgac atggctacac cgaattcagg tgaatgctgc atgccaagcc
     2641 attgagcaaa gataacagca atcccaatca gcacaccacg atacgttaca gaaatcaatg
     2701 ttttgtccgt aaagattcct gaatttggat ctcttggcgg gcggttcatc gtagcaggtt
     2761 cagctttttc tgtacctaat gcaattgctg gcagtgaatc attgactaaa ttgatgaaca
     2821 acagttgtaa tgctgtaaat ggattggacc agtcagcaac taaggcaaag atgatcgcaa
     2881 tgatcgcacc taaatttcct gcaaatagat aagaaatagc tttcttgata ttatcaaaca
     2941 cattacgtcc tacttctact gcgctgacaa tagaagcaaa gttatcatcc gtcaatacca
     3001 tcgctgctgc atcttttgcc acatctgtac ccgttcccat agcaattccg atgtctgctt
     3061 gtttcaatgc tggtgcgtca tttacaccat cacctgtcat agctgagata tgacctttat
     3121 tttgccaagc acgaacgata cgaattttat tctcaggtga aacacgtgca taaacagtga
     3181 ctttttctaa aacttcgttt agttgatctt ctgtcaattc atctagttcc gtcccggtca
     3241 aagccaggtt tccttcttca aaaatcccga tttgttttgc gatagcaaca gctgttgttt
     3301 tgtggtcacc agtaatcatg attgttttga ttcctgcact tcttgcttct gttaccgcct
     3361 gagtcacttc agcacgtgga ggatcgatca tagcaagcaa accaacaagg atcaaatcgt
     3421 cttcatcttc aaatgataaa tcaaaattat caagtggacg gtaagcaaaa gctagtacac
     3481 gtaatgcgcg tgttgaaaaa gcttcatttt gctcttggaa ttttttcatc aattcatccg
     3541 tcatcggaac gacttcacca tttagcaata cttttttact gcgttggaag acgatatctg
     3601 gtccgccttt gacaaacaag taattttcgt tatcgatcgt gtgtccagta gacattaatt
     3661 ttcgatcaga atcgaaagga agttctgctt gtcttgggta gttgtttcgg atttcctgat
     3721 aaggctggtt taatttttca ctaaagtcga taaacgctac ttccgtaggg tcgcccaatt
     3781 tagtaccgtc ttcactgatc gatgcatcat tcgctaaaac actgatttcg atcaagcgac
     3841 gttcgtcagc tgtccatttt gaagggtcat ttgtgaaatc cccagtatcg ccattaccta
     3901 aaaagtagtc aacaactgtc attttgtttt gtgtcaatgt accagtctta tctgtacaga
     3961 tgatacttgt cgaaccaagt gtttcaactg ctggaagctt ccgaataatg gcatgacgtt
     4021 ttgccatttt attcgttccc aaagataaca cgattgttac gatagactgt aatgcttctg
     4081 gaatagcagc tacggctacg gctacggcaa acatcactgc gctgacgata tctgcagtca
     4141 tatctcctgt accttctcca agataaatac gaaacagttg gatacctaaa atcaataaag
     4201 acaatgccaa aatagccaga cttagttttt tactgaattt atccagacct cgttgtaacg
     4261 gagtgacttg ctcagttgtt gattccaaaa ggccagcaac ttgtccgatc tccgtattat
     4321 tcccagttga agtcactaag aatttaccgc gtccgtaagt tacgatcgtt ccactgaata
     4381 ccatatttgt acgatctccg attggtaccg tacttgtgat atccgttact tctttttctg
     4441 cagaagtcga ctctcctgtc agcatacctt catctacttt taatgatccc gcatctagta
     4501 aacgtccatc agcaggtaca taatcgcctg cgtctaacaa cacgatatct cctaccacaa
     4561 gttcgtttgc cggaatcgtt ttttccttac catcacgaat cactcgagca tcgggcgctg
     4621 aaaggttttt caacgcttct aaagaacctt ctgctttttt tgtttggaca acactgatta
     4681 tagaattcaa catcaatact gcaaaaataa ctaagaattc gatgtagtca cccatgacca
     4741 tctgaatcag tgcgacaatc agcaatacga taaccattgc atctttgaaa gtttctaaga
     4801 acaatttcca agttggcttc ttcggctttt cttttagctg gttcaagcca tctctttcta
     4861 atttttcctt cgcttgcgtg gtgctcaatc catcggttga gctctgcgtt tgctgcatca
     4921 acgagtcgat cgtttcttta tagaccgctt gttgtgcttg ttccttgttt tccaaatttc
     4981 atcccccatt ttttgatctt ggccctagtc ctgtctgaaa aaaaaagaga cctaagacaa
     5041 atacgcattt gtcttaggtc tcaccatttc atacaatacc agaaaaccat ttggttaact
     5101 tactgttgat attatcgcaa caaacgttgc cggttactcc cctaaggaga tattcaactg
     5161 tactaatatc ctaccttatt aacctacaaa aagcaactac tttagcccga tttacctaca
     5221 ttttcatgaa aggagtgaaa agtatattca ccttggtagg cttctttctt ctatcatgct
     5281 atactaatgt tattgtatgt aaggaggaac atttttgtgg tatctagtat tataaccgcc
     5341 ctttatctat taaatgcact tattgctttg gtggctattt tgatcaaacc ccgagatgta
     5401 gcagccattt gggcatggct cttagtattg tttgcccttc ctggggtggg ctttgtatta
     5461 tatttatttt tcggacgtgg attaacggat aagaaaaaat tctatctccg acaaagtgac
     5521 ttgaaagaat tagaaaactt tcagtctttt agaggagata ccattgaaca ttacgatcct
     5581 gacatgggcg ataaagacaa acagcaattt gtcgacttct tctcttcatt aaatcgtatg
     5641 ccgctgacaa gaatgaattc tgtcactctt ctcacagacg gacaagagaa attggattca
     5701 ctgcttcaag atctaaaaaa agccaaacat tcgatccata tcgaatatta cgcatttgtg
     5761 acagataata tcggccagca agtcttacat gttttagaag aaaaagccgc agaaggcgtg
     5821 gaagttcgaa tattatatga tgcatttggc tctcatggta caaaagcaaa agatttcaat
     5881 cgtctaatca aaaatggtgg acatgtccat acatttgtta cctcacaaaa ggcattactt
     5941 cgtttccgat tgaattacca tgatcaccga aaaatcgttg tgatcgatgg aaagattagt
     6001 tataccggtg gtttcaatat tgccaatcaa tatgtaaata caacaaaaaa attcggctat
     6061 tggcgcgata cgcatatacg gattttcggt gccgcttctt ctttgctcca gcttcgcttc
     6121 ttaacagact ggaacgtctc ggtacctgaa gaaaaaaagg tcggctatca tttgaattat
     6181 ttctttaaaa aagcagatcg agatgaatct aagcttgctg atacatccat ccagcttgtt
     6241 tcaagcggac cgaataacga aagggaacaa atcaagcttt catttatcaa attgattact
     6301 tctgctaaaa aacgtgtttg gatacagaca ccttaccttg ttcctgatga aagtgtcatt
     6361 gctgctttaa aaatcgcaac tgcctctggt gtagatgtga aaatcatgat tcccaacaaa
     6421 ccggatcatc cttttattta tcgagcaaca caatattatg ctcggcagct gatcaaggaa
     6481 aatgtacaaa tccttgtcta tgagaacggc ttcctccatg caaaaacatt gataatggat
     6541 gatgaaatct gcatggtagg ttcagcaaat caagatattc gtagctaccg attgaatttt
     6601 gaaacaagtg ctgtcattta cgatcctgag tttttagaag aacttgctac tcagttcaaa
     6661 gaagatgaga cacattgttc atccatgaca actgaaacag tcaaggaaat gtctaactgg
     6721 ctattattca agcaacaaat ttctcgatta ttttctccaa tcctataatt tttttattca
     6781 agtagagaaa aatatactaa gattaaaggg actgtgacaa aaatcttgtc acagtccctt
     6841 cttccgaaca aacggtgttc tcctgccaac tcctcggtca caagtcacaa tattcaacaa
     6901 acatgagaag ctgttttttg aacttgttcc ttgtgactcg gagctggacg gcaggctcac
     6961 gacctctttc ttttactcta attcaccagc tgcccaatga ttgatgggtc catatttgtg
     7021 ccctacattt atttcttcac gaattgcacg gtttacatag tcttttgcca atcggatcgc
     7081 ttgttcaact gtttttcctt tcgctaactc agctgcaata catgcagata gcgaatcgcc
     7141 agttccgttg atccggtcgg taggataaaa tggtgcattt aaccaaaatg attttcctga
     7201 ttcgagcaat acgtagtcat tgactgtttc ttgctgcgca tctttagcat gatgtccttt
     7261 gatcataacg ttttttgcac ccatatcttg cagttctttt gctgcttgca caaaatcttc
     7321 ttcagctgct aatgtccgtc cagtcagctt ttctgcttca taaaaattag gtgtcagtac
     7381 ttcggccaaa gggacaagct tttctttcaa gctttgcaaa gcagattctt ctagtaaaag
     7441 atttccatgt ttcgttacga ttacgggatc cacgactaat ggtccaaaat catatttttg
     7501 gtaatttttt acaactgtct caatcaactt tgaatctgct agcatacctg ttttagctgc
     7561 acgtatttga aagtccttcg caatcagttc aaattctttg tcgataaaat ctgttggcaa
     7621 tgtcacactt gctccgattc cgtaagaatt acctgcaaca catgcggtca tgacagaaac
     7681 accgtaaaca tttcttgtaa agaatgtatg catatctgct tgcatccctg cgcttccatc
     7741 gctatctgat cctgcgatcg tcattgcttg tacatactga tcttccatca aatcgattcc
     7801 tccattttta taaaaagatt tttcataatg ctgttactgt ttctatcata tgtttttcaa
     7861 agtatccctc agtaaatata ccactacctt ttcttggtaa tcaaatttat ttgaaaaaac
     7921 atttgtaata aatcagaaaa tagttgtcat ttgtttgtat tcgctattct aataataaat
     7981 aaccactatt tgatttatat atatttcagt atccttcttg ttttcatata accaaaggcg
     8041 ctatgatttc catatgaaaa aaataaagtc aggtgaaaaa aatgaaactt gaggataaaa
     8101 aagtcatttt gaagaaaatc aaacgactac cagaccaaat agatggctat acgatctgtt
     8161 ctttcaaaaa agacaggagt ataaaagctt atacgcaaaa cacagaaaaa ggaataatct
     8221 atttcctaca tgaagaaggt tacgataccc aaacctttat gtttactgaa aaaaaagaat
     8281 ttcataaaca gatgaaaaaa ctgattgaaa aagaatttcc aagaagtcat aaactctatg
     8341 tgaatcagca attttaactt ctaactgttc ttta
//
LOCUS       DAMEET010000098         8273 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.099, whole genome shotgun sequence.
ACCESSION   DAMEET010000098 DAMEET010000000
VERSION     DAMEET010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.099"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            7..624
                     /locus_tag="PB780_002241"
     CDS             7..624
                     /locus_tag="PB780_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734268.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
     gene            1644..2813
                     /locus_tag="PB780_002242"
     CDS             1644..2813
                     /locus_tag="PB780_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDE0734269.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            3286..3840
                     /locus_tag="PB780_002243"
     CDS             3286..3840
                     /locus_tag="PB780_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDE0734270.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(4013..4486)
                     /locus_tag="PB780_002244"
     CDS             complement(4013..4486)
                     /locus_tag="PB780_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDE0734271.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            4498..5034
                     /locus_tag="PB780_002245"
     CDS             4498..5034
                     /locus_tag="PB780_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDE0734272.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            5074..5445
                     /locus_tag="PB780_002246"
     CDS             5074..5445
                     /locus_tag="PB780_002246"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDE0734273.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            5729..7000
                     /locus_tag="PB780_002247"
     CDS             5729..7000
                     /locus_tag="PB780_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDE0734274.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            7245..8270
                     /locus_tag="PB780_002248"
     CDS             7245..8270
                     /locus_tag="PB780_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDE0734275.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
BASE COUNT         2945 a         1173 c         1456 g         2699 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataaag tactacctat taaaggtagt actttattta atttagtata aattaacata
     1321 ctactatatt ttaggtttca tattatataa attaataata tactaccatt tgttggtagt
     1381 ataatttaaa ggtaataagt tattctttta ttactatgtt tgggttttat agcaaagaaa
     1441 gtttaataag agtttggacc tagcaaacaa tattaatgag gtcattaaat cttattatct
     1501 ttagtgatat aacgaaatat ctacacagac aaaatgatag cgtaaaatat tttgtgtaaa
     1561 tagatattta gggtagaaaa aaagaaagtc cattctgtaa aattaaagtt accacaaccg
     1621 aaattttata ggaggacttt ctcatgccca attttactac agaaattatg gaaacactaa
     1681 tcaataaagg tgatttagat gaattatttc gatgtcattt agaattagct gttaattcat
     1741 tattgcaggc tgaattaaca gcttttcttg attatgaaaa atatgatcgt gctgggttca
     1801 attcaggtaa ttcccgtaat gggaattatt cacgttcatt cagaacagag tatggagaat
     1861 taaatttggt aattcctaga gatagaaatg gtaagttttc tcaacaaact ttacctgctt
     1921 ataaaagaac caatgactct ttagaaacca ctattattca gctatttcaa aaaggaatta
     1981 ctatgtctga aatctctgaa ttgattgaaa aaatgtacgg gcatcactat acgccacaaa
     2041 ccatttcaaa tatgactaaa atagtttctg aagatattat tgcctttaaa gaaaggtcct
     2101 tagaatccag atactctgtc atttttatgg atgctactca cattccttta aaaagacaaa
     2161 ctgtttcaaa agaagcagtt tatattgtaa ttggcattcg attagatgga accaaagagg
     2221 ttcttgggtt tagtcttgct ccaactgaat ctgcttatgt ttggaaagag attcttcaag
     2281 acctaaaaga ccgtggttta aaagaggttt tattagtcgt aactgatggt ttaagtggca
     2341 ttaacgatag tattcatagt gtttatccaa atgctcaatt tcaacaatgt tgtgtccata
     2401 tatctagaaa tatcgctcat aaggttcgtg ttagtgatcg acaagaaatc tgtagcgatt
     2461 tcaaattagt ttatcaagca agttcaaaag aagaagcaaa taatcaaata aggtttatga
     2521 tagataaatg gaaaaagcag tatccacgag tggttaaatt gctcatgaat cctgctatat
     2581 taacctttta taatttccct ccgtcaatca gaagaactat ttattcaact aatttgattg
     2641 aaggttttaa caaacaatta aagaaatata ccaaaagaaa agagcaattt cctaatgaag
     2701 aatctctgga gagatttcta gtatctcaat tcaacaacta taatcaaaag tttctatgcc
     2761 gagttcataa aggttttaaa gaaatacatg atacattaga atcaatgttt taacttgatc
     2821 aacagaatgg gcttttccat ttacacaaaa ttattgacgc tcccgacaaa attatgatct
     2881 tgcgtaaagt tataaaatat taataaaaat attttattaa tgaaaagctt ggtagcacgg
     2941 ttattcatat gctaatttat ttcactttat agtaagggga gttcttagta tattgttcaa
     3001 acttaaattt catataattt aattatgtaa attagctatt ctaagaactt ctacggataa
     3061 ctgcccgtct aaaatttaaa taagagctta gaatcttcat ataagtaagg ttaatttttt
     3121 tgtacaaaaa agtcggaata ttctgagtta ctgaaatgat ataaaatcat aaactaaatc
     3181 ccttgatttt attgactttc aaaagtttat atatataagt taatgattat ggtaaaatat
     3241 atacataatc attaactcag aatattttta agaagggaaa caatagtgag ctataatgta
     3301 caaccgttaa ggacaaatga agagatacaa gattttcagt tttggctaag aagaacaagt
     3361 aatccggaaa gagatagttt tttatttttg tttggaatca ataatgggtt acgtatgtca
     3421 gatattatag gattaaaagt tggagatata cgaggcaaaa gtaagcctat tattgttgaa
     3481 cataaaacag gtaagcgaaa accgattttt atagataatt tgagagaaga aattttatta
     3541 tatactgaag gtaaagaaga aaatgattgg ttatttccga gtcggcaaca aggtcgacat
     3601 ataacaaggg atcgtgtcta tcagatatat gcagatatag cagaaaaatt aggacgagat
     3661 gatataggaa cacatacatt aagaaaaact tttgggtatc actactacaa gaaaactaga
     3721 gacattgcta ccttaatgtt tatttttaat catagctcgc aagcaattac aaaaaggtac
     3781 attggtataa ctgaagatga aataggagca tcacttcgtg gatttaaact aggtgtataa
     3841 tttaaaaatt tttataaaca gtaaagacaa agattaaaag ctatttttat ctagaacgat
     3901 tttatttcat aaagttcgcg gaataaaagc tggggttact ggtacttggt ataggttcgt
     3961 gttatataaa ctaagtaatt aaataacatt tttaggttgg aaaaggatga aattaaaaaa
     4021 agagaatcaa tacggtctca aaaacgcaaa cgctgcggaa taagaaaaat actgataact
     4081 caacgtttag cagactatct aatagggata gtctgctttt ttcgtttttg accatacttt
     4141 tgaccttctg caaacttcct tgcttttcct ctgtagaact catgaaatct gcaaaacgaa
     4201 ttgccgtttc ctctgtctgc tttttcgcga tatgggtgta tatatcatcc atagtggttt
     4261 taatatctgt atgtccaagt cgaacataca cttctttgac agacgcacca gattcaaaga
     4321 gcagactaca atgggtatga cgaaaaccat gaagtgtgat atgttttaag ttattctctt
     4381 taatgatgat tttcaaattg tgatttacat agtctaaata aaggggctta ttttcttctg
     4441 atgaaaacaa aagttgttca ccatctttta taggtttgat tctcattttt agatattatg
     4501 cccgcacgta ttcatcacat ttctattgtg aatcgcttta ttcgaccgac atttgacttt
     4561 tatcataata tactcggttt aaaattacta atgaaaacaa ttaatcaaga cgatcatacc
     4621 atgtatcacc tgtttttttc agataatcac caccgtgtgg gaactgaact gacttttttt
     4681 gaagtacaag aagggaacaa ccagtttttt ggaaccaata cgattgaacg aacgatacta
     4741 aaagttcctt ccgaagcatc attgcatttt tgggaaatat attttgaaac acaaggcgtt
     4801 tgccattatg gaatcgaatc attttctgga cgacctatct tacgttttga agggccagat
     4861 gcaacacgat tagctttagt tcccttacgc gaatttgaag atattgatga ttatttcccg
     4921 tatgtcacag accagatccc tacagagcat gccattttag gaattgatgc cattcaacta
     4981 cgcgtacaat attccgatgc gactgaacga gagttattgt cggtaggctg gtagcacaaa
     5041 gaatacgtca gtttcttttc gggtcatcaa cgagtgacaa tcttagaaaa tcaagacact
     5101 cagttctatc aagaagttca gattattcaa gatcgtgaga atccagttgc cattgaaggg
     5161 attggtagcg tccatcatgt tgcgtttggc gtagaaaata aatctgattt acaacgcatc
     5221 gacaaacaat tacaagagcg caattttata aattcaggaa taaaagatcg tgaatttttt
     5281 gtatcacttt attatcgtga cccaaatcaa ttattgattg aaattgctac aggggaagga
     5341 aacctagatg caaaagcgta tgaaaatcaa agtccacgct ttgaagaaat tcctttattt
     5401 ttaccgcagt acttaaattt cattcgtgaa caagtggagc aataattagc taatcaaatc
     5461 catgacgaag agtaagaggt tgactcataa gtcaaaaaat aaagaaaaac gcatcaaatc
     5521 cattctaaag aatgttgatt tgatgcggtt cctattttta taaactatca ttttcgacta
     5581 ataaaactac ttatgagtca gcctctcttt ttttcttgat ttttatacct ctttctaggt
     5641 aaactaaaca tctgccaaaa ataccgtgtc tcattgaaac attctcaact atttcaaaat
     5701 aaaggggagt accaaatgaa cgagtatatt gaaattatcc acgcaagaga aacaacttaa
     5761 aaatgtcagt ctcaaaattc ccaaaaataa aattagtatt tttaccagcg tatctggttc
     5821 aggaaaatca tcgattgttt ttgatactat tacccaagaa gctggtcgtc aactgaatga
     5881 cacctatgat agttttaccc gtttattttt acctaaatac agtcgtccag aggttgatga
     5941 gattgataat ctttctacgg cgattgtggt caatcaaaag aagattggtg gcagtgcgcg
     6001 ctcaacgtta gggacgatta ctaacatcaa tgcgttattt cgtgtgttgt tttcatgttt
     6061 tgcaacacct agtttaggat atgcaaatgc ttattcattt aatgatccta gtggaatgtg
     6121 ttctgtctgt cagggaatcg gtaagacgat tgcattttta ataggattgc tttatttttc
     6181 ggctattgat aatgctgatt ttgtattttc tgatccattt accgcagaag ttttgtctgg
     6241 aattgaggaa caggcaaaga atcatggtta ttttgtattg gtgcataatg ttaattcagc
     6301 agaagatgtt aaaactattc agagaaattg gcgttttgag gggttcattg ttgttggtgt
     6361 acgtgtaaaa gaatttttgg aattaaataa cgtttttgat ggtcctgttg tttatattga
     6421 tacgcatctt tcaaaagaga cattaagaga acttgaacaa caaatgaatc gtttatttgt
     6481 aaatacaaat gattaccaag ctagtacatt ggcagtggaa catttgattc attatggtca
     6541 tgagaaaata gcgtttcttt cttttgacta cattgtaggt gaaccaagtg ttattcaaga
     6601 acgacatcaa gcttatatcg atacattaaa gaaatatcat ttaaccttta aatcgcattg
     6661 gatatataac aactctgaat ttgagcgaat ttatcaagaa ttagctgaat ttactgcaat
     6721 tgttgttact ggagattatt tagcggctaa attagtaaaa tttttaaaaa ataagaataa
     6781 acgagtgatg gaacaattat cgattattgg atttgatgat attacttttg cagaattaat
     6841 ggatcctgcg ttgacaacaa tacgactaaa tccaggtaac aagggggcga ttgcttttca
     6901 ccaactgatt ggactggcca aacaagaaga aataaaaaat caagtactac tattagaggg
     6961 agaattgatt tctcgagact ctgttcacaa aatcaaataa aaaagctctg tgaaataagt
     7021 atagttaaca ataaaatatc cgaacgatag agagaatgaa ctgtgatcta gcagttcctc
     7081 gtcgcaaatt cactagaatt agcattattt actctaaatt gttcggatat tttttcttgt
     7141 ttttaattat gattacagtg cctattttta acgaaatttg cttattttca aaatgtatga
     7201 ttatatgtta gtaaacactt tcaaatatga aaagaggagc tattatggta ggaattaggg
     7261 atgttgcaaa acgggcgggt gtgtccattg caacagtatc gcgcgtgcta aatgaggatc
     7321 cgacactttc agtaaccgat gaaaccaaac gaaagattgc agaatcggtt aatttttatc
     7381 attatgtcaa aaaaagtccc gttgcaaaaa atttaacaaa tattgcatta ataacgacag
     7441 tttctgaagt agatgagttg gaagatccct attttcgaac gattcgcaga gggattcaag
     7501 tcgaagcaga acaaagcaaa gtcaatttga agaaaattat tcgtttgtca gaaacaccgt
     7561 tggagcaaga cgatttgcta aaatgtcaag gtgtgttgat tattgggcag gtgctatctt
     7621 ctgtgattga agaagtatca gcaattaatt cgaatgtcgt agtgattgat gaccctagta
     7681 ttgaagctga agtagatgca gtttatactg atttaacaaa agcaacgaat cagcatttag
     7741 atcgattgta taaaaaagga caccgaaaca ttgcgtttat cggaggagga cgtgttcgtt
     7801 tagatagcca aggaaataaa cacgtgtcag atgatgatta tcgaaaaata gcctatgaga
     7861 cgtggatgaa acaaaaagga ttagaaaaat atagtcaaat ttatttagaa ggctggacca
     7921 cacttgacgg aatgcaggca gcagatttac ttttgaaaaa acatgctaaa gataagttgc
     7981 cgactgctat catggtagga aatgatccta ttgctgtggg ggtttatcgt tcacttcaaa
     8041 aaaatggtct acatattcca gaagatattt cgattgtgag ttttgataat attgaagttg
     8101 ctgaattttt aacacctgca ttaaatacgg tgaatgtgaa tacagaagaa attggaaaac
     8161 tagcagttcg catggttaat gaacgaataa aagaagttag aaatgtttcg atcaaagtga
     8221 ttgtttctaa taatattatt gttagagaaa gtgaaaaaaa tatcgattaa aaa
//
LOCUS       DAMEET010000099         8104 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.100, whole genome shotgun sequence.
ACCESSION   DAMEET010000099 DAMEET010000000
VERSION     DAMEET010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.100"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(225..1178)
                     /gene="menA"
                     /locus_tag="PB780_002249"
     CDS             complement(225..1178)
                     /gene="menA"
                     /locus_tag="PB780_002249"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDE0734276.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1191..2294)
                     /locus_tag="PB780_002250"
     CDS             complement(1191..2294)
                     /locus_tag="PB780_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDE0734277.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2384..3340)
                     /locus_tag="PB780_002251"
     CDS             complement(2384..3340)
                     /locus_tag="PB780_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HDE0734278.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3621..5555
                     /locus_tag="PB780_002252"
     CDS             3621..5555
                     /locus_tag="PB780_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDE0734279.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5886..6302
                     /locus_tag="PB780_002253"
     CDS             5886..6302
                     /locus_tag="PB780_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDE0734280.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6316..6882
                     /locus_tag="PB780_002254"
     CDS             6316..6882
                     /locus_tag="PB780_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HDE0734281.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6905..7891
                     /locus_tag="PB780_002255"
     CDS             6905..7891
                     /locus_tag="PB780_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDE0734282.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
BASE COUNT         2424 a         1571 c         1626 g         2483 t
ORIGIN      
        1 cggtattaag ccaaatttat gcaaaaatgt ggaaagccat tttcacaaaa tttttcttaa
       61 tactcgaaac taagcgactt tttcacaacc tcctgataaa cggtgttcaa aagcttgcat
      121 ctgcggtaat aagcccaacc aaacgaaaaa tataaggagc tatttttatt tggctgttct
      181 tattacttga tgcagggcag ttttttcaca acctcttcat gctgttaaat ggcttgcata
      241 atgattgtta gtaatagccc caatacataa ctggcattga ataagaccat atttttgatg
      301 gaatagctga aactgatagg ctggggtaac gtttgcttga attgatccaa atgcttcttc
      361 acaattggca aggtcaagaa ggtgaccaag atcggccatt catagatacc gctcaacaac
      421 cccattaaaa tcacgaaata gcaagcatac atcaatagct ggaataagac tgttcccgca
      481 ggacgtccga tataaaaaac tagtgtatag cgatgatttt caatatcacg gtcaagatcc
      541 cgcaaattat tagccaacat gatattggca atcgtaaaca ccaagggtaa ggaagctaat
      601 acgattgctg ctatagccca aagatttcct accaaatcaa attgtccaga tgaaaagttg
      661 actgtcagat catagacatc tgaaccgaat gtattgacat aaacgaccat ggcaaaaatt
      721 ccaaggccca tagtcacccc actgaaaatt tcccctaaag gcattcgaga taaaggaatc
      781 ggtccgaatg tatagaatac tccaataaaa caacagataa atcccatcaa aagtaaaagc
      841 caacccgtat tgatcgttag aataacgccg ataacaaaaa caaatgcaat catagctaaa
      901 atcatgtttc gaatcaatac aggagataga tttctgcggc caatgacatt ttcttcatac
      961 ttatatactt cagaatgagc cttttcataa tccatgtaat tattgatagc ggtagtcgct
     1021 aaatcaaaaa ctagcattcc aataaaaaat aaaaaagtgt ttagccaatg tatctcatga
     1081 aaaaaagcga tagagaataa tacccctacc gcaaaaggaa atacactagc cacctttgtt
     1141 cgtatttcaa ctacttctaa aaagattttt agtgtcatgg ttcgtactcc ttattttagt
     1201 tcagaacggt cacccatcgt gtaatgggag tcctccgata atttgaaggt gtctttgatt
     1261 ggatcggtca catatacttt atcgtctttt gtcacgaaga tcgcttccgt cccgttattc
     1321 aattcgcttt cgacgtattc cagtcctttt ttcacaccca ttgaaaagac tgctgtagac
     1381 aacccgtcac catcaattga tttatctgtt atgatcgtga cacttgcgat atcattgtcg
     1441 aacggataac ccgtttttgg atcaaacaaa tgatggtatt ttttgccatc tacttctaaa
     1501 taacgctcgt aaattccaga ggtaactaat gttttgttcc gttctttaat agaacctaaa
     1561 acagatccgc gagccatgtt cggatcttgg atgccgactg tccagtcttg gttttcgcct
     1621 cttgggctgt ggcctaagac atagacatta ccgcccaagt caacgatggc agtggtcact
     1681 ccttgttttt tcaagacttt cactacttcg tccgtaatgt aacctttggc gattgcacct
     1741 aaatcgatga tcattccttt ttcttttaga taaacagttt tttctttgtc atttagttcg
     1801 atcttatgat aatccactaa tttcaatgcc tgatcgattt cttcttggga aggttttctg
     1861 gcgtcatcaa aaccgattcg ccataattgt gtgattgcac caatcgccat atcgaatccg
     1921 ccttgagaat cttggctata ttcgtaggcc cgcttgacta acgtgtagac gtcatcagaa
     1981 acttttactg gtttgatccc agcttgctca ttcacttcat cgatctcaga acctttttga
     2041 ttgatcgtga ttttatcacc aagttctttt actcgatcga aggcaggttt caatgctgat
     2101 tctttgcctt catcatacac acggatacga acgtaagtcc ctaacaagaa ctgttcatca
     2161 gaatatggat ctttcaatat cttggtagct gctgaacttt cagtggttgt tttatttgct
     2221 gagttgctgc agccagttag caccataaaa gagatgccca gtgtgattaa ggaaaatagt
     2281 agttttttcc gcatcttttt cctctcttca ttttgaaagg tgagacgact gcctaatctg
     2341 gctatcgtcc caccctccaa cttttattag tatgattatg tgattatttt tctgttacga
     2401 tattatctac ttcgattgtt tgagtgtcgc ctttttcagc tgcattcact aattgttgtg
     2461 cgtacatgat gaatgaatgt gagctgtgtg tagcacctgt aacaacttct acattagcag
     2521 gtgagccatc ttcttcgccc attgctttga cgaattcgtc gttcagagta gggatgtaag
     2581 tttttggttc tgtaccgctt tttgctttca tattttcatt atattctgta tcatcttgtt
     2641 ttgatttacc gtctttgtcg atataatcaa agtttgattc agttactttg ccgccagcaa
     2701 ctgtcatttc gaattgcaca cggtagccgt tagagtagtt cttttcttca agcttgtaag
     2761 tgccgtcttt caaatcagca ccattgtcga tttcgattgt atctgtgtta ccagcttgag
     2821 cagcttggat caattgttgt gcgtagtttt ggaagctttc agatgagtga gtagctccag
     2881 ttacgacttc cacaccgctt gcgctttgtg cttttaagaa ttggtcattc aactcaggaa
     2941 tgtatttttc tgggtttgtg ccagattttg cttccatgtt tttgttgtat tctgcatctt
     3001 ttgtttttga ttcgccgttt tcattgacgt tatcatattt tgattcagtg attttgccgt
     3061 cttttacaac catttcaaag actgcacggt aaccatttga ataatttttt tcttctaatt
     3121 tatatgtgcc gtcttgcaaa tctccgccag ctacaacttt tgcagatgat tctgttgtgc
     3181 tttttgcagt cgaagattgt gctgtagatg attcagaaga agagtttgag cttccgttgt
     3241 catctgcgcc gcaagcgcct aacactagag ctgaaaatgc taatacggca aatccagtta
     3301 caaaacgttt tttcttcatt ttaatacccc accattccat ttctagtcca caatttttct
     3361 ttgtgattca ttatacattc taatcttttt agcctataag tcaactattt ttgataaata
     3421 acccatgtaa tatttcaaac gttttctttc agcaaatttc gtgaaaaaac gctttatttt
     3481 aaaatttgaa aaaaatccaa aaggagttag acaaaagaac agaacatcta tataatatat
     3541 ggagattgtg taataagtat caaactaaaa gaattatcat atattcatta tatagatagt
     3601 ctataaggag cggatgatca atggcaaagc aaaatattgt cgttgtagga gcaggatatg
     3661 ccggtgtttc agctacaaaa tacttggcaa agaaactgaa aaaagaagat gtagcaatta
     3721 ctctgatcga tcgtcattcg taccatacta tgatgaccga gttgcatgaa gtagctggcg
     3781 gacgtgtaga accagaagca atccaatacg atctgcagca tttgtttgct cgtcaaaaga
     3841 atgtacaact tgtcacagac actgtaatcg gtatcgataa agaacgaaaa atcgtcaaga
     3901 ccaaattagg ttcctatcct tttgatcagc tgatccttgg aatgggcgga gagccgaatg
     3961 actttggtac acctggtgtc aaagcacacg gattcaccct ttggtcattt gaagattctg
     4021 taaggattcg ggaacatatt gagaagacag tagcaaaagc tgcattggaa ccagatgatg
     4081 aactgcgtaa agccatgctg actttcgttg tctgtggttc tggatttacc ggtattgaaa
     4141 tgattgggga actgatcgac tggaaaaatc gcctagccaa agattataaa ttagatccaa
     4201 acgaaatcac actgatggtc gtagaggcaa tgccgacgat cttgaatatg ttggatcgaa
     4261 atgatgctgc aaaagctgaa cgctatttgc gtaagcacaa cgtggaatta ttattgaatg
     4321 caccaatcgt agaagtggct gctgatcata tcaaattgaa agacggacgc aacattccga
     4381 ctcacacact gatttggaca gctggtgtca aaggaacttc tgacgctgct gactttggat
     4441 tagaatcagc acgcggacag cggttgatcg ccaatgaata tatgcaggca aaaggctacg
     4501 aagataaaaa catctatatc attggtgatt tagtctttta tgaagaattc ccaggaacac
     4561 cgacaccgca gatcgttcaa gctgctgaac aaacaggaca cacagccgca gcgaatatcg
     4621 tggcttcgat caaaggcgga gaaaagcata aatttaaagg aaactatcaa ggatttatgg
     4681 tttctattgg tgccaaatgg ggggtagcta acctatttga taagatccat ttaagcggat
     4741 ttttagctat cgtaatgaaa catatcgtga atttgaaata cttctttgat attcgttctg
     4801 gttattatat gttccaatat ttaatgcatg agtttttcca tatcaaagat gatcgcaacg
     4861 tgactcgagg acattcttct cgttacggca atgtcttatg gagtgtgcca cttcgtattt
     4921 tctatggttt agtttggttc gttgaagctt ccaaaaaaat cattgggaat ggtgaagtgt
     4981 tcaaaccaag tacttggttt ggtgaaggat catggttcac tggaaacagt gtgttccctt
     5041 ggacatggga atacaatgta acagatgttg ccagtggtgc ttctgctgct ggtgatgcaa
     5101 ccacagctgc cagcggagca ggggcaagtg ccggagaagc agcgactcaa gctgcacatt
     5161 ttggtttgag ttatgcatac ggtgaagaac caatggctgt gattgagaag tcaccagact
     5221 ggttcacaaa gatcatgcaa gtgatcatgc caaacaatga tgtagcaatg ttcttccaaa
     5281 aattcatggt attctttgaa attgccttgg cattagcatt gattgctgga ttattcacat
     5341 ggctatgcag tgcgacaacg attgtattgg tgatcacgtt ctgcttatca ggcatgtttt
     5401 actgggtaaa catttggttc atttttgttg ctttcgcatt gatgaacggt tccggacgtg
     5461 cgattgggtt ggatcgctgg gtaattccat ggctacaacg tacactaggt aattggtggt
     5521 acggtagacc aagttcccag tacggcaaga aataaataga taaagaacgt tatttgaatg
     5581 caagatttca atagaaaggg gctgagacaa accgtcacag cctccttttt atgtaaaatt
     5641 aaaggaataa gatgcccgag ataagcctcc atgagcaaga cacaggtgtc acaacctctg
     5701 ttacaacctc ttgttataaa cggtgttcca cctgctgctt ctcggtaata agcccaaaga
     5761 attagaaaac atggaaagct gttttctatt cttcgttctt attactcaaa gcaagacaca
     5821 ggtgtcacaa cctcttgttt ttatgctaaa attgggtgag ttaatctgga aaaaggagaa
     5881 caaaaatgag ccttaaagaa tttgctgaaa aaagtctgct caaaccttgg gatgttttta
     5941 ttattttgct tttgactgtt ctttcttttt taccagtcat gatttttggt cttcagcaaa
     6001 acaccgcaac agcagaaaaa gaagcggtac tgcgtgtgga tggcacagaa atcaaaacat
     6061 tccctttgat ggctggagaa gagagcaaca catatactta caaagatgaa cacggagatt
     6121 ataacttgat cgagatagat ggcgatcgag tacggataaa agaagcagat tgcgatgacc
     6181 agatctgcgt acgtcgaggc tgggcaacga aaaatggcga aaccatcgtc tgtttgccac
     6241 ataaattagt gatcgaagtc cgttcaacag atggaggtga cgaagacagt ctgatttatt
     6301 agaccttcac acaccatgag taaattacaa aaaatgattt atatttcgct acttgttgca
     6361 caaggagtga tcattggttt attggaaaat atgatccctt atcctttttc ttttgcgcct
     6421 ggtgcaaaac taggcttagc caatctaatc acgatcgttg ccatttttac catgaaaaaa
     6481 caagacagct tcttactact ttggctacga ctattcctga ctactttgct aggcggaacg
     6541 atttccacat tcctttatag tatgagtggt gctttgctta gttattttgg aatgttattg
     6601 gtcaagcaat taggaccaaa acgtgtcagt atcatcggaa ttagtgcggc cggcggtttc
     6661 atgcataatg tcggacagct tttgatggct tctttcattg cacaatcatg gacagtcatg
     6721 ttgtacttgc cagtcctttc agttttaggg attttatcag ggattgcaat cgggatcgca
     6781 gcgaattatc ttttacgaca tgtgcagaca cttcaacgat tccaaaaaga ttatgagcag
     6841 ggaaccaaac aaaaatggag cccaatgttt accaaaaaat aatcgtcaca caaggagttt
     6901 ttttatgaag ctacatccaa tgtgggaaaa ctatcctgct ttaagtaaag aattgacaga
     6961 aacactaaaa cggatggaag aaactgtccg cctgaaaaac aaacctgtcg aacaagccat
     7021 caaagaaacg atccatgccg gcggaaagct gttgcgtcct gcctatcagc tattgttctc
     7081 acaatttgga ccagagcaag atcgagacaa agccatcgct ttggcagctt ctatcgaaat
     7141 gctccatacg gccacactga tccatgatga tatcgttgat gaagcggatc tgcgacgtgg
     7201 tcagcctaat atccgtagtc agtttggtaa tgtcgtttct gtctatgcag gagactattt
     7261 atttgtctgc tgttttaaac tattgtctga ttactccact tccttgaaaa gtctccaatt
     7321 gaattctcgc agtatggaaa aagtattgaa tggcgaatta gggcagatgg atgatcgcta
     7381 taattatgaa ctttctgtaa aagaatattt ggaaaatatt tcaggtaaga cagctgaact
     7441 gttccaatta agttgttctg ttggcgcttt tgaaagtgga acaagcgagc gcttcgcaaa
     7501 aaaagcagga gatatcggac tttctatcgg gatggccttt cagattatcg atgatatttt
     7561 agattataca aaagagagcc aggaaatcgg caaacctgta ctagaagaca tgcgtcaagg
     7621 ggtctattct ttaccgttga tctactcttt gcaaaaaaac aaaccaaagc tactacctta
     7681 tttagagaag aaagcggcaa tgacagaaga agatgtcgac gccgtacgca aaatcgttga
     7741 acatacagga ggcgtggaag aagctaggaa gttagcggcc tcttacacag aaaaggcttt
     7801 gaaggaaata aagaaattac ctgccacctc tcttcgaaca aaagagaatc tcttttccct
     7861 tacccaactg attttggatc gcaaagactg aatagggaaa ggttcgatac gtaaataaat
     7921 ttctcagaag gaaatctacg tgttttttga caaattgaat tgtagttctt gtatttatga
     7981 taaaaagcaa aaataaagag caacgtccgt tagcgacgtt gctctttttt atcttgaata
     8041 tttttttacc ttttcgattt cttataactc ataaaagtga ttcttaaacc gattacaagt
     8101 aata
//
LOCUS       DAMEET010000100         8068 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.101, whole genome shotgun sequence.
ACCESSION   DAMEET010000100 DAMEET010000000
VERSION     DAMEET010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.101"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..884
                     /locus_tag="PB780_002256"
     CDS             <1..884
                     /locus_tag="PB780_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HDE0734283.1"
                     /translation="ISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTY
                     NDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVL
                     GVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMT
                     LAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILA
                     VVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGW
                     IIGAILCLITFLLYGNA"
     gene            932..3061
                     /locus_tag="PB780_002257"
     CDS             932..3061
                     /locus_tag="PB780_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HDE0734284.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3036..3725
                     /locus_tag="PB780_002258"
     CDS             3036..3725
                     /locus_tag="PB780_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734285.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(4133..4819)
                     /locus_tag="PB780_002259"
     CDS             complement(4133..4819)
                     /locus_tag="PB780_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDE0734286.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(4955..5149)
                     /locus_tag="PB780_002260"
     CDS             complement(4955..5149)
                     /locus_tag="PB780_002260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734287.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5199..5639)
                     /locus_tag="PB780_002261"
     CDS             complement(5199..5639)
                     /locus_tag="PB780_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDE0734288.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(5643..6488)
                     /locus_tag="PB780_002262"
     CDS             complement(5643..6488)
                     /locus_tag="PB780_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HDE0734289.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            6637..8055
                     /gene="pepV"
                     /locus_tag="PB780_002263"
     CDS             6637..8055
                     /gene="pepV"
                     /locus_tag="PB780_002263"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HDE0734290.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
BASE COUNT         2481 a         1515 c         1714 g         2358 t
ORIGIN      
        1 gtatttcagt accggttgca ctgttctcat ttatgggctc gattatggct ggaatctttg
       61 ctaatatcgt caatttcaaa caatatcaaa cgatttatgc tggtttcaat ccagcggctg
      121 aaagctatac ttataatgac tatttccaaa ttggtatgat tgggatgatt gtaagcttag
      181 ttgtggtact aacggtcgct aatatatcaa tgaacaaaaa gaaacgctat gcaatggcag
      241 caaacgtacc agctgaaggt ggcgacgcac caatgatctc atggttagcc gtattattac
      301 cagttttagg ggtcgttctt ttagatcttc aaatcatctt aggttttatt ctagcaggta
      361 tctgggcatt gctgtttact ggaaaattgc gcggaggtta taaagaaatc tgtcgtcagt
      421 ttgcgaaatt atttacagat ggcgcagtcg atgttgcacc aatggttggt ttcttgatga
      481 ccttagcgat gttcaataat tcagctgcgt atgcttctcc ttatttttca gctatttttg
      541 gagattggat accgcaatca ccattagtct tggcaatcgt atttgctata ttgacaccac
      601 taggattttt ccgcggtcca atgaatctag tcggttcagg ctcagctatt ttagctgtcg
      661 tgttagctgt taatccaacg atgtcgccag cctttttgtt cccgttattt gcaattacaa
      721 cgattgctcc tcagcattta gatattacac aatcttgggt tgcttgggga ttaggttata
      781 caaaagtaac ttctcgagaa tatatgaaga aatccatccc aactggttgg atcatcggtg
      841 cgatcttatg cctgatcacg ttcttattgt acgggaacgc ctagtaaaca aaacgtcgat
      901 aaaaacgatt agataaatgg aggaaaagaa aatgaaacgt tttgtgcgaa tgggaattga
      961 tgttggcggt acacatacga aagcagtcgc aatcgataat gcgacgcacg aaatcatcgg
     1021 aaaatcttca gtgaaaacaa cccacgatga tgtccgtgga gtagctgcag gtgtcgttca
     1081 gtcttttcaa aattgtttgc gggaaaataa tatcagtcca gaagatgttg tgtttgttgc
     1141 acattctacc acacaagcga ctaatgcact gattgaagga gacgtagcaa aagtcggcgt
     1201 aatcgggatg gcaaaaggtg gattagaagg ttttttagca aaacgacaaa ctagattgaa
     1261 cgatatcgat ttagggaata agaaaaaaat agagattgtc aatgcattct tacctgtcaa
     1321 aaacttaaat gtagaccgtg tttcagaaac gatttcttct ttagaaagag aaagagcgga
     1381 ggtcttggtt tcttctatgg catttggagt agataatggg gaaccagaac gagttgttta
     1441 tgaagcagca tctgtgaaag cgatccctac aacgatggct tctgacatca caaaattata
     1501 tggattgaca agaagaactc ggacagcagc aatcaacgca tctattttac caaaaatgct
     1561 agatacggca acctcgacag aagactccgt acgagaagca ggggtcaatg tttctttgat
     1621 gattatgcgc ggagacggcg gagtaatgga aatcaatgaa atgaaaaaac gacctgtttt
     1681 aaccatgctt tccggtcctg ctgcatcagt catgggctct ttgatgtact tgagagcgtc
     1741 taatggtgtt tattttgaag taggcggaac aacaaccaat atcggggtca ttaaaaatgg
     1801 acgtccagca atcgattatt caattgtagg tggccaccca acatatatct cttcattaga
     1861 tgttcgggtt ctaggtgtgg caggcggttc gatggtacgg gctaatcagt caggaatcat
     1921 cgatgtcggt ccacgttctg cacatattgc cggtctagat tatgcagtat ttacggagac
     1981 agaaaaaatc aaaggtccta aggttgaatt tttctctcct aaagaaggag accctgctga
     2041 ttacgtcaag gtagtgatgg aagacggaga agaagtgact attacaaata catgtgcagc
     2101 caatgttctt ggattagtgc aagaagaaca tttttcttat ggaaatgtcc catctgcacg
     2161 aaaagccata caggcattag cagactattg tcatacgacg gttgaagaca tcgcagagca
     2221 gatcatggaa aaatcctatg ctaagattga accggtgatt ttagaattgg cagacaagta
     2281 tcacttggag aaagatcaga tttctcttgt aggtgtaggg ggcggcgctg cctcattgat
     2341 tacttacttt agtaataaaa tgggtgtgaa atattcgatt cctgaaaacg ccgaagtgat
     2401 ttcttctatt ggtgtagcac ttgcaatggt ccgagatgtt gtagaacgta tcatcccttc
     2461 gccaagtaaa gaagatatac gatcgttgaa aaatgaagcg atgaataaag cgattgaatc
     2521 aggcgctaca ccagaatcga tcgaagtcca cgtagaaatc gatccacata cttcaaaagt
     2581 aacggctatc gctactggtt ctacggaagt gaaagcaacc gatctaacca aggagatcac
     2641 aacagaagaa gcactagagc ttgctgcaga ggatatgcga ttaaataaaa atgaagtttg
     2701 cttattggaa aatacacctt tcttttatgt atgcggcgaa cagaatcgtt caaaaaatgc
     2761 tggaagtctt cggatcatcg atcaaaaagg atttatcaag gtccaacgtg gacatgcttc
     2821 atgtatgaaa acaactgcag ccaattatat gacagctgtc gaacaacttt gggaagatat
     2881 ggcagtttat caaactgaat tgattgcccg accagaattt tatctctgct taggagcaag
     2941 agtctctgat tttacagcga cggatctgga acaactacag cttttgatgg atctggaagt
     3001 atctactatg gaaccggagg aagaagtgat cgtcgttgcc ggaaatatca aacaaacata
     3061 agttagaaga gttactaaac gaattacaac agatccccga agaaatttgg ggattctacc
     3121 agtttcggcg agatttattt tggaaaaaga ttcctttgtc caagcagaaa attcttatac
     3181 aacaatcaat cgattgtggt atcgaaacgg catgtagcat caaaaaaaag tacccttttg
     3241 ccgatgtagg agaaatctgt gaacaaatgg ctgttccaat cgtttcctgt gaatcagagc
     3301 aaataaacga acgaatcact tttgcaactt atgcagaaga tgaagggatc cgattgatga
     3361 cggagccgtt agaaaaatta aaatgttcag gactagcttc gatctctaaa gaaacagcgc
     3421 aagctttgat tattggacat gaactatttc accacatcga agcttctgtt aagggaattt
     3481 atacgcaaag cgaaaaaatt gtgctgtggc ggttgccttt ttatacacat caatcaaaca
     3541 ttcgtgcatt gagcgagata gcagcgatga gtttttcaaa agagatgaat caaagcgatt
     3601 tttcgcctta tgtattagag gcagtcttac tttggccata taatgaaatt cgtagccaag
     3661 ggatcttaga agaaataaaa gaaatagaaa aaagatgtac ggagtatgat ttagattaca
     3721 aataaagaga ctgtgacaaa agtcgtgtca cagtcctttt tttccgaata aacggtgttc
     3781 tcctgtcaac tcctcggtca caagtcaaaa tattcaacaa acatgagaag ctgttttttg
     3841 aaattgttcc ttgtgactcg gagatgaacg gcaggctcac gacctcttgg aaaaaggtgt
     3901 tcaaaagctt gcatctgcgg taataagaac agccaagtta aaaacataag aagctatttt
     3961 tacttggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct caataaacgg
     4021 tgtgcaatca gctgaccctc ggtattagct caaaaaaaac ggcaaaatat gaggaactat
     4081 tttgccgttt atttctctaa tactcaggtc aaaacgctga ttccaaaacc tcttatttga
     4141 tgggtctgtc ctgaatatct tcaaaataat cggggtaatt atgaatcacg tattcttgct
     4201 cctcgataat catatgtaaa tgcatcgtgc aaagaaatcc gacttcatca aagttatgat
     4261 caatcattga ttgaagcatt gtctgatgtt catctaatac tcgaccccag tctaattctg
     4321 aaatcgtcaa acgtaaccat ctgaaacggt tcaagtgcgt actataactt tctagccaat
     4381 cccaaatttc tcgttttcca gcaatttcat aacaagttcg gtgaaaatca gtatccaaat
     4441 caaaaaaagc ttttttattt tgagcgatca ctgcctgatc ggtttgttct agtatttttt
     4501 ctagtttagc ttgatcttgc ttggtcattt tcgcactgca ttcttgcagg atcggttttt
     4561 ccaacttttc ccgcgtataa cgggctaatg aagcagatcg gatatcgatt tttgaaacat
     4621 aagtgccgga ctgtggaatg gtttttacaa gctcttgatt gcgcagttgg atcagtgctt
     4681 ctcgaatcgg tgtacgcccg atagctagtt gttcttctaa cactttatcg gagattttac
     4741 ttccgggtgc taactcggaa taaatgattt gtttacgtat agattcataa gcttgctgtt
     4801 gcaaattatt gataaccatt taaccttcct ttctatgcag atataatcta ttataacata
     4861 aatcgcttac aaatcgtgtg ggcataaaaa cacagtctgc aaaaaaagcc ctgataggca
     4921 tgcctattag agcctcgaaa agaaaaatat tgaatcaatc tggtcgtacc tcttggatcg
     4981 gtttgacacg accgccgatt cttttagcaa aagcttcgga ttcttttttg tctttgaaaa
     5041 tgagcagatt gtctaagttt ttttcccgag tgcgacagcc agttttatcg atgtaaccgt
     5101 gcattacttt aacgacatac atggggatca cctgcttttt tcttttcatt atacgaacta
     5161 ctcgacacaa tacaagaaaa atgcttagac ggtctttatt aagaatttac atcatccaaa
     5221 gaagcgtacg cttggaataa ctgttctaac tccggattga ctttgctcag cttcactaat
     5281 cgttgcgaat cagaaagtaa aaaacagtgt ttactactgc cagtagtttt taattttttg
     5341 tcttcagttc ccacaatttc gtagctaaaa tccaaccgtg ttcccgtata cttttggata
     5401 gttgggtgga tgctcacttt ttctccgtaa tggatcattt ctttatagct gcaatttact
     5461 tccagaacgg ggatgatgat cccagattct tcgatttttt tatagggaaa ccctagatgt
     5521 accaataagg ctacccgagc ctcttcaaac cagcggatat aattgctgtg atgaataatc
     5581 cccatttgat ctgtttcata ataaaaaggt tctcgcacat aacctgtaaa tttctccatg
     5641 actcatgcct cgttttccat tgatttttta caaaaataat tcatccactt cggaagtgct
     5701 tcgctgattt gtgtcggcaa aacaatatat tgttcttttg ccagctggtc tccaatgaaa
     5761 ctatgaagat aaacagctgc tgcaatcgtt tggtcatttt gaggaaactg ggctaaaaat
     5821 cctgtgatca tcccagctaa agtgtcaccc atgccgcctg ttgccatagc agcagaacct
     5881 aaaggattgg tgtagaagtg ctggtgacca taaatttttg tctcatgact tttcaaaacg
     5941 agcacagcac cgatttcctg ttgcttcttt ttgttttctt tatcccgttg ttcttgtatc
     6001 ggtagagaac tcaaccgttg ccactccatt tgatgaggtg taagtacagt ttgttcggga
     6061 tagggtagca aaagatgatt agcaacaagt aaacttaaag cagagccatc aatcaccagc
     6121 cattgttctt ttgattggtt ttctagtact tgtttgaaac gttccaaact cttagaagaa
     6181 gtacccaatc cgggaccaat caagagtaca tctgcttgct ttattacgga aagaaactgg
     6241 tcagtatccg tccaatctac aatcattgct tctggcagat gactgtgaag agcactccga
     6301 tttttttcat cagttactac agtcgtgaga cccgctccac tgtgtacggc agctaaagca
     6361 ctcataatga ttgctccgcc atattgttca ttgccgccaa tcaaaacgat acgtccaaat
     6421 gttcctttat gtgaaatctt tggacgaggt gtgatgactc ttttcactag ttgttcgttt
     6481 atcagcatcg aaaaacctcc ttttactcat tatagcgtat atttttttta gagaaaaaca
     6541 gataagaaaa ggaattgtca gattttttga actatttttt tctgtaattt gtggtatcat
     6601 gaacacgaag acttaaataa gtggaggatt aactttatga caattgattg gcaaaaagaa
     6661 gtagaagcac gtaaagaaga cttgcttgaa gacttgaaaa atcttttacg tgtcaacagc
     6721 gagcgcgatg attcaaaagt aacaccagat gcaccatttg gacctggacc tcgcgacgca
     6781 ttgaaacata tgttagctta tggcgaacgt gatggattta aagtaaaaaa tgtcgataac
     6841 tatgcagggc atattgatct tggcgaagga gacgaaacgc tagggatatt tggacacatg
     6901 gatgtcgttc ctgcaggaga tggctgggac acagatcctt atgaaccagt aatcaaggat
     6961 ggaaagatct ttgcacgcgg atcaagcgat gacaaaggtc caagtatggc agcttactat
     7021 gcgatgaaga tcattaaaga acttgacttg aaactatcaa aaaaagttcg cttcgtagtc
     7081 ggcagcgatg aagaaagcgg ctggggcgat atggcttatt atttcgaaca tgaagaagaa
     7141 ccagattttg gtttctcacc agatgctgaa ttcccaatca tcaatggtga aaaaggaaat
     7201 gtttctttag ctttacgctt caaaggcgac aatgcaggag attatgtctt gaaatctttt
     7261 gtttccggac ttcgcgaaaa tatggtgcca ggaacagcaa ctgcagcact tcaagtacca
     7321 agtgcagatg cagctatcgc gatggaagaa gcattttacc aattcatcga agcaaatcca
     7381 gtaagcggaa cgattgaagc ggacaatacg tatgtaaaaa tcgaattagt tggtaaaggc
     7441 gcacatggtg caagcccaca atctggtatc aatgcaggtt ctttcttggc attgttctta
     7501 gataactatg aattcctagg atctgctaaa caatttatcc atgtagcagc tgcttatgtc
     7561 catgaagatt tttatggaga aaaactagga gtcgcttatg aagatgaaaa aatgggcaaa
     7621 ttgacaatga atgctggact atttgctttt gaagaaaatg gtacagagga agcgaacttc
     7681 atcaatatga atttccgttt tccaaaaggt gtaacagtag acggattgca aagtgacatc
     7741 gaacaaacag tggggcaaga aggcgcaaca gttactcgag gtgctcgcgt aatggaacca
     7801 cattacgtac caatggatga tccattggtg gctacattat tgcaagtcta tgaagaccac
     7861 actggagaaa aaggctacga acaaatcatc ggtggcggaa cttacggacg tttgttgaaa
     7921 cgtggcgttg cttatggagc gatgttccca ggatatactg atacaatgca tcaagcaaac
     7981 gaatttatga gtcttgatga cttgttcaga gcaacagcaa tctatgcaga tgccatctat
     8041 cgtttggcga aataaaaata ctaaaaaa
//
LOCUS       DAMEET010000101         7741 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.102, whole genome shotgun sequence.
ACCESSION   DAMEET010000101 DAMEET010000000
VERSION     DAMEET010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.102"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(32..1180)
                     /gene="nagA"
                     /locus_tag="PB780_002264"
     CDS             complement(32..1180)
                     /gene="nagA"
                     /locus_tag="PB780_002264"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDE0734291.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1256..2092)
                     /locus_tag="PB780_002265"
     CDS             complement(1256..2092)
                     /locus_tag="PB780_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDE0734292.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSQDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2276..3493)
                     /locus_tag="PB780_002266"
     CDS             complement(2276..3493)
                     /locus_tag="PB780_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDE0734293.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     ITSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3642..3821)
                     /locus_tag="PB780_002267"
     CDS             complement(3642..3821)
                     /locus_tag="PB780_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734294.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3876..3959
                     /locus_tag="PB780_002268"
     tRNA            3876..3959
                     /locus_tag="PB780_002268"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3910..3912,aa:Leu,seq:gag)
     gene            complement(4199..5569)
                     /locus_tag="PB780_002269"
     CDS             complement(4199..5569)
                     /locus_tag="PB780_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734295.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLI
                     MGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSLQKNEFMQFGLTVLGICTFI
                     ITVINR"
     gene            complement(6284..6574)
                     /locus_tag="PB780_002270"
     CDS             complement(6284..6574)
                     /locus_tag="PB780_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734296.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(6782..6985)
                     /locus_tag="PB780_002271"
     CDS             complement(6782..6985)
                     /locus_tag="PB780_002271"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734297.1"
                     /translation="MNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLE
                     KAKDEVEDTADKSQQIVEEHLRK"
     gene            complement(7082..7474)
                     /locus_tag="PB780_002272"
     CDS             complement(7082..7474)
                     /locus_tag="PB780_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HDE0734298.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEIN"
BASE COUNT         2366 a         1529 c         1182 g         2664 t
ORIGIN      
        1 aagtttagtt ttaaggttaa cttaaattat attatgcgtg gaaacgttcc acgccatcta
       61 gataagttgc ttccaatgtc atatcagggt tcaatacgat aaagtctgca tcgcgtcctt
      121 gagcgatttt tccacatgta tcatcgattt tacagctgat tgctggaatc agactagcca
      181 tcatgactgc ttgttctggt gttgctaatc cccaatctac aacattttta attgcttctt
      241 tcaatttcaa tatacttcca gctaagttgc cagattctag acgtgctgtt ccttctttta
      301 ctactactgg aaattcgcct aaattgtaat ttccatcagg catcccaccg gccatcatac
      361 agtctgtaat taatgcgaca tgatcatgtc ctgctttttc cattaggatt cccgcagcat
      421 ttggatgtac atgatggcca tcacaaatca attcagaaaa gacatgttct aaagataata
      481 acgcacccac catacctggt tcgcgatgat tcaatccacg cataccattg aaagcatgga
      541 caaatacact ggcgccagct tcaacagctt tttgcgcttc ttctagcgta gcgttactgt
      601 gacctagaga aacaactact ccttcttcag tcacttgttt gacaaactct tctacacctt
      661 gacgttccgg tgctaaagca atttttttga ttaaaccgcc tgaagcttct tgccattcat
      721 ggaaagtatc caaatcagga tccccgaaat aacttgggtt ttgtgcccct ttgtgttctt
      781 ctgtgaagaa aggaccttca aaatagattc ctttgatttt agctcctggt gcttcctgat
      841 acattttgcc gattgtctca gctacatctt ttaaacgttc tttgcttgac gtcaatgttg
      901 ttggtaaaaa tgaagtcact ccacaagata acagaccttc agacatcact ttgatgcctt
      961 ctgcatcatt atccattaca tcatgattca tatagccgtg aatatgtgta tctaccaaac
     1021 caggagcaat ccatttgccg ctttggtcaa tgatgttgac ttctccctca ggtagttctt
     1081 tggtgtattc accaaaaagt ccatcggtaa tttcaagata gcctgcatgt ttgacacctg
     1141 acgctaaaaa gaatttgtca gcatagataa aagttctcat tctattctct ccctttgttt
     1201 ttctacctct aaagtactct tatttagaga agaagtcaag aatggtctac accatttaca
     1261 aattttttga aatttgtttc acagcttgtg ctaagccttc atagaatgct gctctttctg
     1321 gttcttcttt acgcttgtat attaagcttt taagcacaaa taaatcattg aggtaatagt
     1381 agctattctt ctcttgagcc caatagattc cttgattcac ctgttctaac gcttcgtcga
     1441 tttcattttg atggaataag aaatttccat aattataaaa aatttgcgaa aattcacttt
     1501 ttgatcgttc gtcagctgat tggtgcaaca gttgaatgct tcgactcaaa taatagcgtg
     1561 cttcagccgt ttttcctgca acaccatata tttttccaat gcaactgatc agttgtattt
     1621 ctgtgtcaga aacatggatt ctttcttttt ggctagagta gcttaatgct tctttcaaat
     1681 aataaaggga ctgagagata ttttgacgca acgtgaattc gcaaacaccg agataataat
     1741 aatagcgttg aaagtcagtt gcttgataca gattttcaga aatatcagaa gattttagta
     1801 cttgttctaa cttccgataa tcacgttttt ggaaacaact atccaacagt tcaaataatt
     1861 tatcgtttgt ccgagtagtt tttcgagaga gattcatcac atgatcaatc gttaccccta
     1921 gccgttgaca aatctgcatc atcaccaata cattcggcag ttcctcatta ttttcgatcc
     1981 gacttaatac actttgtgaa caaatatctt gcgaaagcat tttttgcgtc attttttgtt
     2041 cttttcgaat ctctttaatg atcgtgccaa aggactcgat taacgtttcc ataagtatcc
     2101 tccccaaaat atgcgtattc gcattctatt cgtattttac tttaggaagt cgcaaacttc
     2161 aagaaaagac ttgcaactaa ataatatact ttagttattt aagaaaactc cactagaaag
     2221 agagcctcag tgtgtttgtc tgtggccctc ctactgaaaa atttggtttt taaaatcatt
     2281 tttggcgata ggttgctagg aattctctca ttttcttagc tgtttgtttc aaatcatcca
     2341 ttttaggaag ataaacgatt cgaaaatgat ctggctggtt ccaattaaat ccaccaccat
     2401 gtacaagtag aatatgatgt tcatgtaaaa agtccaaaac gaatttttca tcatccaaaa
     2461 tattgaatcg ctttgtatca atttttggga atatataaaa agcagctttt ggttttacgg
     2521 ctgaaagacc aggaatatca ttgattgcat tatagatata ttctctttgt tcatagatcc
     2581 ttccgcctgg caggagcagc tcatcgacac tttgataacc gcctagcgcg gtttggataa
     2641 tttgctgaga caatacattg gaacacaaac gcatggagga caacatattc agcccttcta
     2701 tatagccttt aacgtgtgac ttatctccac ttaggaccat ccagcctact cgaaatcctg
     2761 ctacacgatg agatttggat agaccattca gcgtaacaac gaacaaatca ggcgctaatg
     2821 tagcaatcgg aatatgttct agaccatcca tcactaagcg atcatagatt tcgtcggaat
     2881 aaataatcag gttattttgt cgagctactt caacgatttc ttctaataat tcttttggat
     2941 aaagtgcccc agtaggatta ttaggattaa tgataacaat agcttttgtc cgactcgtta
     3001 tttttgatgt gatatcatca atatcaggat accattctgc ctcttcatcg caaatataat
     3061 gaacaggtgt gcctccagct aatgcaatag aagccgtcca caacggatag tccggcattg
     3121 gaaccagtac ttcatctcca ttgtccagca acccctgcat acacatcgtg atcaattcgc
     3181 tcactccgtt gccggtgtag atatcattga tcgtcacatt cggaaaatgt ttcagttggc
     3241 aatattgctc aattgctttt ctagcagaaa agatcccctt ggaatctgag tagccttctg
     3301 agtcacgggc attcatgatc aaatcacgaa tgacttcgtt tggtgcttca aatccaaaag
     3361 gtgcgggatt tcccgtgttc aattttaaaa tactaatgcc ctcttcttgc atccgctccg
     3421 cttcttctag aacgggaccg cgaacatcgt aactgacacc ttctaatttg ttggattttt
     3481 caaattctct catcacttag ccaatccctc atttcaaaaa gttagaactt tccaaatttt
     3541 ttgttattta ctatttcaat atcatactcc atcctagaaa attttgccac ttatttagga
     3601 aaaacgtaag tattaaaaaa acacaaacaa gcgttaagaa cttaattgtc atgatagtca
     3661 ttccatagat tcgctgccca atccattgca attccacaaa tgattcctag cataaaacca
     3721 agcaaaaaac gtccgaatgc aagtccaatc aatacaccta agatcattcc cagtcctgaa
     3781 aattctccgt catatttctc catcttaata agtcctccca tttttgagct tgacaactat
     3841 gtaaaacctc gctatcatta ttgtgttatt attacgcaga tgtgttggaa ttggcagaca
     3901 agcatgattg agggtcatgt gagcgttagc tcgtgtgggt tcgagtccca tcatctgcac
     3961 tacaactcgt gaagttaatt ttgcttcacg agtttttttg tttgaacgtt tctttatgcc
     4021 attatataca tatcactgta tcctttacaa aaagaacgta caatcatgta aacagcctga
     4081 ggtgagtata tgaaggtcgg aaacaagcta ctcgtagaaa cttgcaagtg ttaaaacaat
     4141 taatcgaaaa acgagtaaaa tccctcacct aatactagag cgagggattt tttgtatatt
     4201 atctgtttat caccgttatt ataaaggtac aaataccaag cacggttaat ccaaattgca
     4261 taaattcatt tttttgtaaa ctcatgcttt tcattagctc tttaactgcc attagtacta
     4321 agatggcgat aacaacataa agtatatagt acgtccgcat tccggaagcc catactgtgg
     4381 gagaaaatcc cataattaac cttgaacaaa atcccattat catcaaaata actaaaaaaa
     4441 tagaagtttt cttattgtca aaacttaaat aaattccaac aattaaacat agtaataatg
     4501 ccaagactac taaagtagct atccaagtac ctggataaaa aagtgacagt ttcgttccta
     4561 actctgtaaa gagattattc aggttactac tactccaaat cattgccctt ttatttccat
     4621 tgacgtaagt aaagctttga cccattgtat taccaaaata aataatcagg tttaagaaaa
     4681 acggtaatgc agtcaatata cgcacataat agttttgaca tttcctatat gttagaagga
     4741 aaattaagaa aaatagcaat aaaaataaga tattcatgtc taaaaaaaac ggctttccaa
     4801 atgatgaaat acctaaatct aacttattaa gaaagttgaa atttttatat tcaggaaacc
     4861 atttattaat ttcatgtgca gcacgtaatg cattccctgg agttgtcaaa gagaaaatta
     4921 attctgctaa gcttattatt gaaaatactg ataacttata attatatcgt ccattgacaa
     4981 tcaaatataa actaactata cttgttagaa caaaaaaaca ggcgttgact tgttcttgat
     5041 tagttaaaaa tatcaataaa ggaatactta cccaatatat ttttctattt atttcttctc
     5101 ctcttaagag ttgaaaaaac ggataaaaag ttagtaaact aaatgctact ggccactgat
     5161 agttcaaggt ggttgctctc caaccggttt cccccattac tgtcaatggt attaaacagt
     5221 atatagagca aaccaataat aaatttttaa catttatttt ctcaaaaaca tacttgcaaa
     5281 gtaatactac aattcctaac ataatcgtag aatttaataa tttccatagc ataaagtgtt
     5341 tagaaaagaa cattaagaat agttctataa taattcttga actccatgtc ttataatgtt
     5401 ctatcgaagc acccaaatag ccaagttcat ctaatctact tgcataaact acgtcatccg
     5461 ctctcatagt cggcatatac atattaaaga gtaaaaagat accccaaatc cctattagta
     5521 acaagatcag ttctttgtta ttcgttattt ttctattatg tttgttcatt tttatactcc
     5581 ttcttatatt tcctaataga gtataacata gctttaagca ttacttaaag ataacttaaa
     5641 agttatcttt taaatactta attgttaaat accttatttg atgcaaaaga aatctacgat
     5701 agtcaacaaa aaaaattttc attatatcca gaaaaatttt tcttagttaa ggacctatgg
     5761 attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca tattgctttt
     5821 aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt aggtttaact
     5881 gtcgaaccag gacgttctcg tgtgaaagac gaggcttctt caatttactt ttacgactat
     5941 gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa atcctacaat
     6001 tccactacct gagactagta atgactagtc tctttttatg gcctctgatc cccccattga
     6061 gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat ccctcaaatg
     6121 agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta tatatagatt
     6181 gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgaa tccatctttc
     6241 caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt attctttcat
     6301 cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca attttgggtt
     6361 tccctaacac atgataggat ctcacaacaa attgctttct attattataa taacctgtaa
     6421 cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg gaagcaatca
     6481 aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact tgcaaagtac
     6541 ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata ccattatacg
     6601 aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt tttgtctatt
     6661 acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta ctaaaaaaaa
     6721 gaggcagcag tatagattac tgttgcttct ttactaattt aggtttactt gttaagaaag
     6781 attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta tcttccactt
     6841 catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt tcagcagttg
     6901 cttcgtctaa tttcttttgt gtatctgtta actgagtttc tacatttgtt ttttcttgtg
     6961 tcagtgttgc gatttgttga ttcaaaccat ttacagtatt ttgtaattct gaaagttttg
     7021 cttgtgcttc ttgtagtctt gctgtccaat ctgcattcag atgattgatt tcatcatttt
     7081 tttagttgat ctcatctagt ttgacattga tttcattttg cttgctttgt agttgtgcat
     7141 ccttgctgac taattgactt tctaagtttt gtttttctgc tgccgattga tttttttggt
     7201 tttctaattc atttactttt gtttggtatt cttggacatc tgcctttgaa tcttgcaatg
     7261 tctgttcttt ttgtgctagt cgagcggtaa gatctgtaat cgcttgctct ttatctgtca
     7321 atgtatgatc aagctgtgtc agattctcat tgattgtatc aatatctgtc tctccacccc
     7381 acaaaataat ttcatttgcg taccgcgtgc ctgtgtagcc aattagacat agaaatgcaa
     7441 cgacaatccc accagtaata aatttctttt tcatttgcat agctccttaa tccaatactt
     7501 ttacatacta aaagtaagtg atctcaaaca caatgtcaag cgtttttaaa ttacatatgt
     7561 aattttaata taaatactat caaatactca tttaatcctc cttttttatc tatttaaaaa
     7621 atctttttta tagagaaaaa tgcaggttaa tactttctta aataaatatt tatggtgaac
     7681 aaaaacaaaa aagcaagttt tattaaacgt aacccttttc atttcataga ttactgtatt
     7741 t
//
LOCUS       DAMEET010000102         7627 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.104, whole genome shotgun sequence.
ACCESSION   DAMEET010000102 DAMEET010000000
VERSION     DAMEET010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.104"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(14..706)
                     /locus_tag="PB780_002273"
     CDS             complement(14..706)
                     /locus_tag="PB780_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDE0734299.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1143..1712)
                     /locus_tag="PB780_002274"
     CDS             complement(1143..1712)
                     /locus_tag="PB780_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDE0734300.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="PB780_002275"
     CDS             complement(1786..2109)
                     /locus_tag="PB780_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734301.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="PB780_002276"
     CDS             complement(2263..3075)
                     /locus_tag="PB780_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734302.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="PB780_002277"
     CDS             3429..4277
                     /locus_tag="PB780_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDE0734303.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="PB780_002278"
     CDS             4422..5363
                     /locus_tag="PB780_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HDE0734304.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="PB780_002279"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="PB780_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2272 a         1512 c         1371 g         2472 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acctatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aacgtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtgtgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt aggatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcggcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggttcg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaaa
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag ttacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgagaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgaa tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagctttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaacgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tcgaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aaccttt
//
LOCUS       DAMEET010000103         7622 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.105, whole genome shotgun sequence.
ACCESSION   DAMEET010000103 DAMEET010000000
VERSION     DAMEET010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.105"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(105..629)
                     /locus_tag="PB780_002280"
     CDS             complement(105..629)
                     /locus_tag="PB780_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDE0734305.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="PB780_002281"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="PB780_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDE0734306.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="PB780_002282"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="PB780_002282"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDE0734307.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="PB780_002283"
     CDS             complement(2351..3031)
                     /locus_tag="PB780_002283"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDE0734308.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="PB780_002284"
     CDS             3157..4026
                     /locus_tag="PB780_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDE0734309.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="PB780_002285"
     CDS             4052..4567
                     /locus_tag="PB780_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734310.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5207)
                     /locus_tag="PB780_002286"
     CDS             complement(4614..5207)
                     /locus_tag="PB780_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDE0734311.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="PB780_002287"
     CDS             complement(5232..5411)
                     /locus_tag="PB780_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734312.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="PB780_002288"
     CDS             complement(5488..5997)
                     /locus_tag="PB780_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734313.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="PB780_002289"
     CDS             complement(6093..6473)
                     /locus_tag="PB780_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDE0734314.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /locus_tag="PB780_002290"
     CDS             6803..7552
                     /locus_tag="PB780_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HDE0734315.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         2168 a         1580 c         1354 g         2520 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aa
//
LOCUS       DAMEET010000104         7431 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.106, whole genome shotgun sequence.
ACCESSION   DAMEET010000104 DAMEET010000000
VERSION     DAMEET010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.106"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(449..1795)
                     /gene="gor"
                     /locus_tag="PB780_002291"
     CDS             complement(449..1795)
                     /gene="gor"
                     /locus_tag="PB780_002291"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDE0734316.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(1929..3182)
                     /locus_tag="PB780_002292"
     CDS             complement(1929..3182)
                     /locus_tag="PB780_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDE0734317.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(3188..4111)
                     /locus_tag="PB780_002293"
     CDS             complement(3188..4111)
                     /locus_tag="PB780_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDE0734318.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4332..6824)
                     /locus_tag="PB780_002294"
     CDS             complement(4332..6824)
                     /locus_tag="PB780_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDE0734319.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6831..7298)
                     /locus_tag="PB780_002295"
     CDS             complement(6831..7298)
                     /locus_tag="PB780_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDE0734320.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         2041 a         1622 c         1188 g         2580 t
ORIGIN      
        1 atttgttcgg aaaaagtgac taactatgac acaatttttt tcacagtccc ttttttcact
       61 tgatttgatt cgtaccagcg aacttgacga gaactttctt ggtttggtaa ctatatggta
      121 ttcttttaac atcctcgttt gcttcttcta atttatttat acaaagtcac acttttttaa
      181 acatctgttc ttcgatacat ttctatttct tttaaacatc gataccaata ttttgaactt
      241 aagagtgcat accaaatgga ttcatttact cttcgtcagt tacgaagata atcaaaatct
      301 ccaacttccc tacttatttc gttttgagaa ttatcgaagc aatcttacat aaataactaa
      361 aaaacaaaaa accagaaaat cagtatactg attttctggt tttacatggt ttgttcaata
      421 aaaagctgtt ggaactgtta atattaaatt atcgcatcgt tacgaattct tccgctcctg
      481 ttggatggat ggctactgta ttgtcaaagt cttctttagt tgcacccatc ttgatagcca
      541 cggcaaaacc ttgcagcatc tcatcgacac caacaccaat agcatgtaag ccgacgattc
      601 tttcttcttt tccgacacag atcaatttca tttcgcattt ttggcgataa tcatttaatg
      661 caaagtacat tggggtaaag cgtgaacgat aaactttcaa ttcattttcg ccatattttt
      721 ccaatgcttc ttcttctgtc aaaccaattg ttgcaattgg tggatgcgta aagacgactg
      781 tcggaacaag tttatagtca agataagaat ctttctttcc attaaataaa cgttctgata
      841 aacgtctacc agctgcgatt gcaacaggag tcagatctaa tttaccgatc acatcaccaa
      901 cagcaaaaat atgttcatcc gttgtatttt ggtatttatc tactttgata aacccttttt
      961 tgtccaattc cacatttgta ttttccagac ctaatttttc ggtattcggt acacgtccac
     1021 cggcaaaaag tacacaatca ccagttaagg tttcaccatt ttcaaatagc accgtatatt
     1081 cgtcattgtt tttagtaatt tctttagcaa tatgatgtgc gtaagttttt attccagctt
     1141 cttggtacat ttggatcaaa ttgtccgaaa gcattttatc aaacgttcta agtggtcgtt
     1201 cttttcgata tgcccacatg acttcactac ctaaaccacg aaatacacct gaaagttcag
     1261 ccgcaatgta tccagcacct aaaacgattg ctctcttagg taactctgtt aattcaaaaa
     1321 atccatttga atcaattgcg tattctccac caggaatggt cattttactt gcacggcctc
     1381 cagtagcgat caagatttta ggagcacgat aggtttcacc gtttacttcg atcgtttgac
     1441 tatcgataaa agtagcgtaa ccttttatag cctcgacatg attgctatct aatcctcttt
     1501 gataagcacc gtgtagaaaa tcaatatatt tttctcgtct ttctactaga ccggcgaaat
     1561 caacattttt taaatctgct tggataccgt aacttgaggc atctcgttca attgtttcca
     1621 aaatcgtact tgcttgccac ataacttttt taggtacaca accgacgttg acacatgttc
     1681 cgcccaattc attcgcttca atcagcaaaa cttttgcgcc atgcatccct gctctgtttg
     1741 ctgaagcaat accgccagag ccgccaccaa ttacgatgta atcatatatt tgcattttgt
     1801 tcctccttat tgaagagatc aatcaaaacc tttatttaac gatccttttt ctacagaaaa
     1861 caagtgtaac acttttcttg aattttgtat gcgaagatgc ttaaaacagg gatatatcag
     1921 ctttttcctt atttacgttt tcggatcata tctccaggtt tcaccggctg tgcgactggc
     1981 atagttaaaa tcatcattgg atttggtgct cggtcaatgg attcgccttc ttcattatgc
     2041 ataacttcaa cggtttgact gaaatgactg aatcctggac cataaaattc gatttcatct
     2101 cctacagaaa acaagtttct ctgacgtatc gtagcaatct gtgttgcctc atcataagat
     2161 aatacttcac cgacaaattt atatactggg attttacgac gttcaccgaa taattgttcg
     2221 ttttcagttg gtgtattgta gtaaaaacct gtagctaatt ctcgttgtgc tactttccat
     2281 aactcgtcga tccattcttg cttgcagaca tagttttcag gatcttccat ataactgtct
     2341 acagccgctt tatagacatt tgacaccgta gatacataat ggatcgattt cattcttcct
     2401 tcgatcttga agctgtctac gccgttttca atcaattctg gaatatgttc gatcattgac
     2461 atatccactg cactcatcga gaattcttct tccacttcgc ctgtatcagt taaactgttt
     2521 cgttcttgac caaaaggcat atcgaacaat ccatacttcc agcgacaaga ttgagaacat
     2581 ccgccacggt ttgcatcccg catcgacata tggttggaca atgtgcatcg accagaataa
     2641 gagatacaca tggcaccatg gataaatgct tcgatttcgg tatccgtatt tttacgaatc
     2701 tctgccactt cctccattga aacttcacga gctaaaacga cacgttccaa cccttcttct
     2761 ttccagaatt ccaatgtctc aaaattagtt gctgaagctt gagtggataa atggatcggc
     2821 agacctgggg cttctgtcgc acaaatttcg atcaaagctg gatcagaaac aatcactgca
     2881 gaaataccga catcacgcaa actgcggaaa aattcccctg ctccatcttc atttccttca
     2941 tgggtgacca tattagcagc aacgtatacc ttcgcatcat gttcttttgc aaaagcaact
     3001 ccttcagcca tttcttcata agtaaaatta cctgcacggc ttcttaaacc atatgcattc
     3061 ccgccaatat atactgcatc tgctccataa tggatcgcgg tttttaactt ttccagtgtt
     3121 cctgctggcg ctaggatttc tggtcgtttc aattttcttt ttactgtcat ttctttcatc
     3181 atactcctta tttgatagca gaaggatcaa tataaaagaa tcctgtatct aattcacggt
     3241 tttcaggatg caatttactg atttcgctag tcattttttc cgctttttct tgatcccagc
     3301 taccttctaa aatttgtttt tttgcttcgg cgaatagtcg agtgatgttt acgaattttt
     3361 cgcctggagt aaataatccg tccaatttcc atgtacagta atgatgctga taaagttcat
     3421 tcaattcatt cgagagattc agatcattgc tggcaaaaat atgtgtgcca tgactatctt
     3481 cataaataga ataatgtgtc tcttcttttt taggttccga gataaaaaga cctctttcac
     3541 gatctttttg ttcgtcttgt ttcgtatagt tatagtaatt ctgtagcagt ggacgtttgg
     3601 attgatggat gcatgtagca ccatacacaa gggtttctac aggaatatcg agtttttctt
     3661 ccatcgcctt catctcttca aaaggaactt cacgagcaag tactgctcca actgcgcctt
     3721 ttttcgccca gaaattgatt tgacgtgcgc tcgttactaa tgtttcagca tcataaatat
     3781 acggtatacg caattcagga ttttttttca tcgtataaat aatacctgta tcaccaactg
     3841 tgatcacatc tacgtttatt tcttttaaaa aagccaaata ttcaggtaca tttttcattt
     3901 tttcaggatg aatgatccca tttacagcaa tattcgcttt tttaccatac ttgtgtgcta
     3961 attctaccaa ttgtcgctgt tcttctcgtg taaatgaagc aggcagacgc aatccaaatt
     4021 gctcttcacc gaaatatagt gtatctgcac ctgcagcaag caactgttct gcttgttcaa
     4081 tagtttctac tgtggttatg agttcgatca taattttctc ctcctacaac ttcataatag
     4141 tagcatagct ctaaaaactt tccaatactt cttctattta aagtcggatt tttgaaaatt
     4201 atttcacaaa aaatcaaaat aaagttcgat atattctgtc caaatataaa aagagattgt
     4261 gacaaaaatc ttgtcacaac ctcttaatca agtcttacta ttataaaaat tcagctacac
     4321 aggccaagta actataggga acctactgtt tcctttttag gtccgcgaac attcaatgtg
     4381 atcttacctt ttgaagcacc tactgttacg tggtcaccta aacgaatttc tccgccaagc
     4441 aaagcctcgc tcaatcgatc ttcaatttct ttttgaagtg cgcgacggat tggacgtgca
     4501 ccatattcag gatcaaatcc agctttcccg atcacatcga ttgcagatgg tgtgattttc
     4561 acttgaatat cttgttcttt caatcgtttg ataatcgctt tgctcatgat ttggacgatt
     4621 gtatggattt catctttact caatgaatgg aagacgacag tctcatcgat tcgattcaag
     4681 aattcaggtc ggaaagcttt ctttaactct tctaagatac gtttttgcat ggctttgtga
     4741 tctttagtaa catcttggac attgaatccg acattttttt cttctcgaat ttgtgtcgct
     4801 ccgatatttg aagtcataat catgattgta tttctaaagt ccacttttct acctttagaa
     4861 tctgttaaat gaccatcatc caatacttgc agaagtaaat tgaaaacgtc tggatgtgct
     4921 ttctccactt catctaagag aatcactgaa tatggttttt gacgaatttt ttctgtcaac
     4981 tgtccacctt catcataccc tacgtatcct ggaggagacc cgattaatcg gcttgtactg
     5041 tatttttcca taaattctga catatcaaca cggatcaggg catcttcact tccaaacatg
     5101 acttctgaaa gtgctttggc caattctgtt tttccgacac cagttggtcc aaggaacatg
     5161 aatgaaccaa ttggacgatc aggatctttc aatccgcttc gtgcacggcg gatcgcacgt
     5221 gaaactgctt tgacagcctc atcttgacca acgacacgtt catgcagaag tccttccagt
     5281 tctaacaagc gttcgctttc ttttttctcc aactgctgta gagggacacc agtccactcc
     5341 gatacaactg tagcaacatc ttcttctgtt acacgatcag aataaccttg ctcctgtttt
     5401 gcttcaattg cagaaacttt ttgcagcttt ttacgtagtg ctttttcttg tctgcgtaat
     5461 tgagcagcat tttcaaagtc ttgtttttgg atcgctgctt ccttttcttg tactagttca
     5521 tcgatttcta gtttgatcac agttgatttt gttaagtgat cggtttgatc caaacggact
     5581 ttcgctgctg attcatcgat caaatcaatt gctttatcgg gtagttgacg agaagtgata
     5641 taacgaacag aaagattgac tgctgctcgt acagcttcat ctgtgatctc cactccatgg
     5701 tgctcttcat atcttgtacg taatccttga agaatcactt ctgcttcttc aggagttggc
     5761 tcatctactt gaatgcgagc aaaacgtcgc tctaatgcag aatctttttc gatatatttt
     5821 tgatattcat ctagggtcgt tgctccgatc gtttgtaatt ctcctcgagc taacgccggt
     5881 ttcaagatat tcgaagcatc aattgctcct tctgctccac ctgcaccaat taaggtatgc
     5941 aattcatcaa tgaataagat tacttgacca tcattataga tttcgtcgat gacctttttc
     6001 attcgatctt caaattcacc gcgatatttc gtaccagcaa ccaaagatcc catatcaagc
     6061 atcatcaggc gcttttcttg catgtcttct ggcacttcac ctaaaatgat tttttgagct
     6121 aacccttcag caatggctgt tttgcctaca ccaggttcac ccaccagtac agggttattc
     6181 tttgtccgtc ggctaagaat ctggataagc cgtttcactt ctctcgaacg accaacaact
     6241 gggtccagct tgttttcttt tgctaatttc gtcaaatcac gtgctaatga atctagcgta
     6301 ggtgttcctt gcggtgtttg ccgtttatct tgtacattta catttctacg acgattcatt
     6361 ccttttccag cagaatcacc agctagcccc attttttgtt tcagaacttg acgggctttt
     6421 gaaaggctga tacctaagtt gatcatgatt cgagtagcca tgatactttc atcttttaat
     6481 aagctcatca aaatatgttc tgttccaact ttttgagaac cagttttctt cacttcgctg
     6541 ctagctaaag caaaaacttg tttcattctt ggtgaatatg gaagaaagac tcctttaggg
     6601 taagaacgga taccaccata accaatcaga tgttcaattt cttcataaat ttccgtttca
     6661 ttcaaatcca tttcacgtaa tacttttcca gcaattccat tttgctcgac cactaaggct
     6721 agtaaaacat gttctgaact aactgattga tgtttcaacc gttttgcttc ttcttgtgca
     6781 atttctaata ctgcttgggc tctttcagta aaaagttctt ccatctaatc tcacttccca
     6841 tcttcataac ttaaacgttc cagaaatgct cggagaatcc gagcacgtgt atgatcttca
     6901 tattcattga aattcaatgt gttttttgca attgcactca acatcaagtt tccttctttt
     6961 tttgtaatta tttggtcttc ataaagtttt tgaataatag caaaagcatt tttctcactg
     7021 attgtatcat caaataactg atttatctgc tcaagtaatt gttttttatc tgaaatcctt
     7081 acttttgcaa ttcgtatata accaccacca ccacgtttgc tttctactag ataaccacgt
     7141 tggatggtaa aacgggtatt aatcacatag ttgatttgtg aaggaacaca gttaaagagg
     7201 tctgccattt caatccttcg aatctcgatc atttcactat tttccagaat tttctttata
     7261 tatgattcaa ttaaatccga ggtattctga tgtgccatct tattcattcc ctttgactaa
     7321 tattgacctt aattataacg aatttgataa tagaaagaaa gaagaacgct ttttgttaaa
     7381 taaatccttt agaatcaaca aaaaaaaata cggaatatac tattccgtat c
//
LOCUS       DAMEET010000105         7243 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.108, whole genome shotgun sequence.
ACCESSION   DAMEET010000105 DAMEET010000000
VERSION     DAMEET010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.108"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..82)
                     /locus_tag="PB780_002296"
     CDS             complement(<1..82)
                     /locus_tag="PB780_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HDE0734321.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQAL"
     gene            416..1273
                     /locus_tag="PB780_002297"
     CDS             416..1273
                     /locus_tag="PB780_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDE0734322.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            1266..2168
                     /locus_tag="PB780_002298"
     CDS             1266..2168
                     /locus_tag="PB780_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDE0734323.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            2201..2551
                     /locus_tag="PB780_002299"
     CDS             2201..2551
                     /locus_tag="PB780_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDE0734324.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            2600..3523
                     /locus_tag="PB780_002300"
     CDS             2600..3523
                     /locus_tag="PB780_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDE0734325.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            3601..4494
                     /locus_tag="PB780_002301"
     CDS             3601..4494
                     /locus_tag="PB780_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDE0734326.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            4532..5095
                     /gene="amaP"
                     /locus_tag="PB780_002302"
     CDS             4532..5095
                     /gene="amaP"
                     /locus_tag="PB780_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDE0734327.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            5109..5297
                     /locus_tag="PB780_002303"
     CDS             5109..5297
                     /locus_tag="PB780_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDE0734328.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            5310..6212
                     /locus_tag="PB780_002304"
     CDS             5310..6212
                     /locus_tag="PB780_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDE0734329.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            6244..6486
                     /locus_tag="PB780_002305"
     CDS             6244..6486
                     /locus_tag="PB780_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDE0734330.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(6742..7113)
                     /locus_tag="PB780_002306"
     CDS             complement(6742..7113)
                     /locus_tag="PB780_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734331.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         2626 a         1226 c         1423 g         1968 t
ORIGIN      
        1 gaagtgcttg gatattttct tctggtatct ttccatttcg atcaagcaag gtaccgtcta
       61 aatcaatcgc tgctagtttc attttatctt cctactttct ttttacatat ctattttcct
      121 gttaccttaa gattaaatga gaatttaaaa aaatgaaact aacttgcttt gtcattatga
      181 attttttcaa aaaacattct taagaaaaag gtggctatat atagtagttt ttggtcaata
      241 cctttgaata aaaagccatg aaaaacgaat agttagcgct taccgatgcg ttttgtgata
      301 aaaaaatttt aaaatattca atgaaaacaa agcatatatt ttgctttaaa accaaatgag
      361 gaatattcta gatgtagaac aggttagaaa aagattagaa aaaggagtga ttttgatgga
      421 gaagagatac ggcgtagttc ctgtggtaac tgcttcagac gaaaattatg ctccttactt
      481 gagcgtgatg atcgcaactg cattagaaaa ttgcaataaa acaagacgca taaaatttta
      541 tgtgattgac gatggtctat cagaatatag taaagaagga ttggaagaaa cggtaaataa
      601 atattcaagt aatgcaagta tccaattcct gacagtggaa aaagatatct acgaagactt
      661 cctagtcagt gaccatatca caactaccgc ttacttaaga atttctttac caaacttatt
      721 agctaaagag gattataaga aagtccttta cttagattca gatgtgttag tactagacga
      781 tatcgtaaaa ctatatgatg agccgttaaa tggaaaaaca atcggcgcta ttatcgatcc
      841 aggccaagtg aaagctttgg aaagattagg aatcgattcc gatgacttgt acttcaattc
      901 aggagtgatg gtgattgaca ttgatcaatg gaataaaaaa gaaattacag aaaaaactat
      961 ccattacctc agtgaaaatg gcgatcgaat catctatcac gaccaagatg ctttgaatgc
     1021 tgtactttat gaagattggg aacaacttca tccaaaatgg aacatgcaga cttcgctgat
     1081 ttttgaacgt catccagcac caaatgaaaa atatgaacgt cagtataaag aaggaaatga
     1141 aaaaccttcg atcgttcact ttacaggaca tgataaacct tggaatacgt tgaaagatca
     1201 tccgtatacc aatttatact taaaaaaatt agcacacagt actctaacga aagtaggcga
     1261 gatcaatgaa taaaaaagaa atagcagtag tcgcaagttg taacacaaaa ttcgtaccac
     1321 atttagctgc attatttgtc tcagtattag ataattgcaa cccgtctaaa tttgttcggt
     1381 tctatgtgat tgacgatgat atcgattttg aaagtaaaca gcttttacgc ttctcagtga
     1441 aaaacgcacg aatgaactca gatgtggaat tcttgaaaat caataaagaa ttctttacaa
     1501 atgtggtaat cagcgatcgt attccagaaa cagcttatta tcgtattgcg attccggaat
     1561 tgttccgcgg cacagaagtt gaacgtatct tatatatgga ttgcgacatg atcgctttac
     1621 aagatatctc taaattatgg cgtcttgact tcggtgattc aatcgtagca gctgttgaag
     1681 acgctgggtt ccatcaacga ctggaaaaaa tgaaaatttc agctaaatca atgcgttatt
     1741 tcaactcagg tttgatgctg atcaatgtga aaaaatggtt agatgaaaat attacgcaaa
     1801 aagtattaga tttcatcgaa cataatccag aaaaattacg attccatgac caagatgcat
     1861 taaatgctat cttacatgac cgttggttac cacttcatcc gcgttggaat gcacaaggct
     1921 atatcatggc aaaagcaaaa aaacatccaa cagcagcagg tgaacgtgaa tacgaagaaa
     1981 ctcgcaacaa tccttacatt atccattttt caggacatgt gaaaccgtgg agcaaggatt
     2041 ttgaagggcc aacgaagaaa tattacgaaa aatatgcagg gatgacggct ttccgttgtg
     2101 tagctaaatt ccctaaatat ccaaaatatg caaaagttca accaaaaaca gatgtagttg
     2161 ttaaataata gaagacaaca actgaagagg gggagtagga atggaaatta caatgtctaa
     2221 aaatgcagta gagacgttaa ttgaaaaggt tggggaaaat acaaaaatcg cactcgcgct
     2281 tatcaatgat tccgatccat ttctaaggga caaaggtgct tttgcaaaag gcagcttttt
     2341 tcaaatcatt ccttttgttt cggaatttgg agaatatgcg actaaaatcg agcatccatt
     2401 attggacatc tatacatcta aattagagca aaattatttc ggtaaacgat tgaacatgga
     2461 cttcaacaaa cagcttgaca gcttttcatt agaaaatgaa atcgcagtac tcgattataa
     2521 tatcaaatta aagaactgct tcttcagcta atagtttcga gaaagtccaa aaaggaggta
     2581 tacattgaga tactactttg tggatgaaga aaagaatgta atagaaacag atgaaaaaaa
     2641 atataattgg ctcgtacttg atagtgcaaa gcccgaagag attgagcaag caatcgaaga
     2701 attcgagctt cctgatgata ttttcgtagg aaccgaatat ccggaagaag tttctcggat
     2761 ggagcgcctt tatgagacga aactagagca tccattttct ttggtagtga ttaatctaga
     2821 tcagtcagat aatcgtatcg aaaaaaaatt aacgccaata tcttttgtac tttccgataa
     2881 tctcttgatt acttgtatcg atagaaaaac cgaatttgtg gatcggctgc tgacaaagca
     2941 tggaaatcat ttaaactcgt ttgaaaagat catcacgtat acattattag atatttacac
     3001 ccattacgtg aaagaactaa gagagatgaa aaagcgaatt gacgctttgg ataaagcagc
     3061 tcgtaaaaca acggaaaatg aagaactgta taaacaggca gatcttgaaa gagacatcgt
     3121 atatatcgat cataccttaa gagatcaacg ggaaacaatg gatcaattat ggaattctga
     3181 aactttcatc aaacgtttag acgatgaaca gctactctat gatgtccatt tgagacagcg
     3241 acaaacagaa aaaatgatcg agatctatcg agatttatta gagagtattg gtgatctatt
     3301 caacgggatg atggacaata atctaaatca tatcatgaaa tatctagatt cagctgcatt
     3361 aatcatttcc gttccggcgt tgttcgcagg gatttgggga atgaatacag gaggattacc
     3421 gggtaaaagt tctgctctag gctttatact cgtagtcttt ggctctttga tagcagcagt
     3481 agttttaggt taccatttat accggaaaga ttatacaaat taaagaatct atttaaaaca
     3541 ggtatcaaac aaagaaattt gttttggcaa tacaatttaa aaactaggag gaatacagca
     3601 atgacagaac caaaattaat cgacccgcga aacttgtacc atacggagaa atttcctaaa
     3661 caagatcagg atacaccagc tttacagcag aatatgattc ctgttcctga ttgtggagaa
     3721 gaatcctatg aaggcaataa tcaactagaa aaccgccgtg tactaatcac tggtggagat
     3781 tcagggatcg gtcgtgcagc agcgattgca tttgctagag aaggggcaga tatcgctctt
     3841 caattcttcc caggagaaga aaaagatgca gaggaagtag caaaatacgt gaaagttgca
     3901 ggacgtaaga ttgtcttact accttttgat cttcgtgacg aacaagcacc taaagaaatc
     3961 atagataaag ccgtgagtga acttggcggg ctagatacga tggtactaaa cgcaggacag
     4021 caaatttctt gtcagtctat tgctgagtta ccacttgagc aagtcaaaga cacatttatg
     4081 ataaacatca tcgctatgtt tgctttagtg aaagaagctg ttccgcatat cccagcagga
     4141 ggagcaatcg taacaacaac ttctgttcaa gcattcaatc caagcgaaca tttattagat
     4201 tacgcagcaa caaaagcttc aatcgctaac tttacgatcg gtttagcaaa acagcttgca
     4261 cctaaaggaa tccgtgtaaa tggtgtagca cctggtccaa tctggacacc attacaacta
     4321 gacgaaggac aaccttctga ttcgatccct gaatttggtc agcaaagttt actagaacgt
     4381 gcaggtcagc caggagaatt agcaccagtt tatgttttct tagcatcgaa taaagcaagt
     4441 tatgttacag cccaaattta cggagtaact ggtggagaag cgatcaactt ataagaagtt
     4501 gatcgtttca cccctatgag gaggtgcgaa aatgaataaa ggtgtaaaaa cgatcggagt
     4561 gatcggaaca ttacttttgc tatctgtgct tttgtttgcg atactattaa gtgcaaccta
     4621 tccattgcct tttcagctag agaagtttcg attcttttcc atcactaatt catacgtgca
     4681 acaatatgta ttttggcttg ccgttgcatt tgctattttg acaattatca cgttacttgt
     4741 tttcctgttt tatccaaaaa caatcggaaa ctttctactg aaacatggtg atggggagtt
     4801 gacgttggat aaaaaagcaa ttgaaggaat ggttcgttct cacttgcatg aagatgaatt
     4861 catccattca ccaaaagtaa atgtcaaagc aacgaaaaac aagatcgccg tcaatgtaaa
     4921 aggggagttg aaacgaactt cagcactcat tggtaaaaca ggaacattga tgaaagaaat
     4981 cgaaaatgaa gtacaacaca tattgggaac aaaagagcca gtgaaagtat cggtgaaata
     5041 cacaggcttt gaagaaccag ctaaacgaag caatgaccgc aaacgagtag aatagggggg
     5101 gaccaaaaat gaaagaacta ttttcgacat acaagctccc tatcatcttt ggggtacttg
     5161 gattgatatt agccgtttta tggatcaccg tgggcttttt caagacctta ttgatcttga
     5221 tcatggcagc gatcggcgtc gctatcggtt tttatctgaa acaaacgaaa atcttagatc
     5281 actattttaa atcttaggag gaaacatcca tgaataacaa taacaaaaat aataacaata
     5341 aaatgaacaa ccaaaaacca gtgtctgcac atccagaccc aacgacacca aaccaatcaa
     5401 atggtgtgaa cgatgaaaag aacaaaacag caccagtatc tgcacatcca gatccagtag
     5461 tccctaacgt tcaacaagga gtagaagaga agaaacatga tgcagaaaag catgcacctc
     5521 atcatccagg aactccagaa tctaaagttc cagaacaaca tgatgcaatc gatccttcta
     5581 aacctgtgga agagcaaaaa catccttctg accatgcgaa agcacaagaa aaacttagtt
     5641 ctgaccacac aaacggacaa ggaaaacttg gttctgatca tacaaataac caaggaaagc
     5701 atgattctga acaggaagat tcaatgcgcg gtgagctgaa ttatgaagat aaagttgtgc
     5761 aaaaaatcat cggaatcgca attgaacaag tagacggttt attatctgca aacggtggtt
     5821 tcttctctaa tgtggcagga aaacttgtga atacagataa tgtaacggct gggatcgaaa
     5881 cagaagtcgg taaaaaacaa gtagcagttg atatggatgt cattgtggaa tatggcaaag
     5941 atatcgaaaa aatctttgaa gaaatgcaag aagtaatcgg caaagaagta aaaaatatga
     6001 cacatttaga agtgatcgaa gtcaacgcta acgtagtgga catcaaaaca aaagaagaat
     6061 ttgaaaaaga tcaagaaacg gtgcaagaca aagtaactga cgcagcgaaa aaaaccggtg
     6121 aatttgcttc gaatcaaaca gataaagtaa aatcagcagc tggaagcggc gcacaaaaag
     6181 taaaagaaaa tacagaacca cgggttcagt aataaggaag taaaacatta ggaggaacga
     6241 aacatgggat ttatttggtc acttatcgtc ggaggaatta tcggagcaat tgcaggagca
     6301 atcacgaata aaggttcttc gatggggatt attgcaaatg tcgtagcagg tttagttgga
     6361 tcagcacttg gtcaagcatt attaggttct tggggaccaa gtattgcagg aatggcaatc
     6421 gtgccatcat tgattggtgc catcatttta gtactaatcg tatcattctt tgctcgaaaa
     6481 ctgtaaaaag acttttaaaa agaggtcgtg aaaaagctgt tcttcatcaa gtaataagaa
     6541 cagccaaaca aaaatagctt ctcatatttt tcatttggtt gggcatatta tcgaagatgc
     6601 aagcttttga acactgttta tacggaataa gaggctgtga caagactttt gtcacagcct
     6661 cttatttatg cgggcaaatt cccaattaga tttttaaaag tttttcctta aatgtgtctt
     6721 tattgataca gagaagcttc tttaataaaa caatgcctcc ctaaattttc ttgagatagg
     6781 aaaggtgaac atacaaaata atacgaccac tttgaaaacc cagatgatcc acaaatggat
     6841 cgtatgatac tgatgaagta aaaagcaatg gatcagaaac tcaaagagat tgaaaaagac
     6901 aaggaagaaa taatatctct tttttactct tgtctgtttt agccaataat agctcgtaaa
     6961 gtaatctaga caaacaaata gaagtaaaca aagtttcgta agagcagcaa tcgtatcatg
     7021 ttgctctgct agacgattga cgagcaggaa aaatgttggt aatggaatat agatcacgag
     7081 taatgaaaga aaaaagtaaa caataaatgg catactattc ctcctaattg tgtgagttcg
     7141 ccctcatttt aaaataacga tatttgtttg tgaagctttt tcgtgtcgta acaaatgtta
     7201 tctaatgtat ttttaaataa ataatcatta ttatcaaaat aat
//
LOCUS       DAMEET010000106         7237 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.109, whole genome shotgun sequence.
ACCESSION   DAMEET010000106 DAMEET010000000
VERSION     DAMEET010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.109"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            99..746
                     /locus_tag="PB780_002307"
     CDS             99..746
                     /locus_tag="PB780_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDE0734332.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="PB780_002308"
     CDS             1139..3784
                     /locus_tag="PB780_002308"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDE0734333.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="PB780_002309"
     CDS             4094..5407
                     /locus_tag="PB780_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDE0734334.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="PB780_002310"
     CDS             5397..6050
                     /locus_tag="PB780_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDE0734335.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /gene="radC"
                     /locus_tag="PB780_002311"
     CDS             6105..6797
                     /gene="radC"
                     /locus_tag="PB780_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDE0734336.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="PB780_002312"
     CDS             6947..7147
                     /locus_tag="PB780_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDE0734337.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT         2394 a         1227 c         1588 g         2028 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttc
//
LOCUS       DAMEET010000107         7098 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.110, whole genome shotgun sequence.
ACCESSION   DAMEET010000107 DAMEET010000000
VERSION     DAMEET010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.110"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..454)
                     /gene="tnpA"
                     /locus_tag="PB780_002313"
     CDS             complement(<1..454)
                     /gene="tnpA"
                     /locus_tag="PB780_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDE0734338.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYRRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            697..2123
                     /locus_tag="PB780_002314"
                     /pseudo
     CDS             697..2123
                     /locus_tag="PB780_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2293..3255)
                     /locus_tag="PB780_002315"
     CDS             complement(2293..3255)
                     /locus_tag="PB780_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDE0734339.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(3257..4696)
                     /locus_tag="PB780_002316"
     CDS             complement(3257..4696)
                     /locus_tag="PB780_002316"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDE0734340.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNKNWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            4881..6827
                     /locus_tag="PB780_002317"
     CDS             4881..6827
                     /locus_tag="PB780_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HDE0734341.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         2323 a         1221 c         1208 g         2346 t
ORIGIN      
        1 attttttaaa tggttctacg tactctcttt tacttataat atatcgaatg cgatcatcag
       61 cttcttgttc acggatgtat ttttcatttg tcttttgatt caatccgact gtactcacat
      121 agtatctctt cgaccaaaag gatttgtttc cgtatttata tttgagattt gcgtgctgct
      181 cgtggattag taccgcacta cgacctttca gataccccat aaaactggat atacatattt
      241 ttggtggtac tcgaacaagc atgtgaatat gatctagcat tgcatgtgct tctatgattt
      301 ccacatcttt catttcgcac aacttcctaa gtatttttcc aagatctttt cgtaattgcc
      361 catagattac ttttcgtcga tattttggtg taaaaataat atgatactta caattccaac
      421 gagtatatgc taaactttta tcgtcatttg acatgaaaaa actcctttcg atctgatgag
      481 cggttggtag ccactcacta gtatatcgaa aggagttttt atcgctaaag atacttcttc
      541 cacgcgtaga aacgcgcggt tttcttgttt cgcacgcttt acgcacaaaa ctgactgaag
      601 tcattaagaa aataaaaggg tctaaaactt ttaagttttg taccgacccc caaaagttag
      661 accaaaaaat ctaacttttg gaggtcggta ttttttatag ctaagtatac ttttgaactt
      721 aaattaaaga tcgttcatga ttatttggat ggtaaaggcg gaagtgatta tctggcaaag
      781 aagtattcca ttaaggttcc atcacaagtg aagcgctgga tcaatgccta tcaagaattt
      841 ggtgaagagg gtctagtaag aaagcgacaa aagaaaaaat attctgttca atttaagctg
      901 gatgcgatag agttatatct aacaagtgag ctgtcctatc gtgaagttgc caacacactt
      961 aacatgaaca accctaattt gatcgctagt tggatgcatc aattccgtga gggtgggatc
     1021 gatggactct caaaaacgaa aggatgtcca cctatcttgt ctaagaaata tgaacccaaa
     1081 aataagaaaa aatctacaac taaagcaacg tctaaagaac gtgaacgcat cgaggagttg
     1141 gagaaacgag tccgttccct tcaaattgaa aatgcctctt taaaagaatt gaggaagctg
     1201 cgaaaacagg aagcccaaca acgacgaatg aagcaatcgc acgaatcatc gcaagcctcc
     1261 gaggaccgtt taaattagtt gagcttctag aaactttaaa gttccctaag gcaaccttta
     1321 tgtactggca aaaacgattg gaccgtaaaa atccagatca agagattgaa gcggaaatgc
     1381 ttaaaattcg ggagaagcac aaggattatg gctgtttacg gatgaccaat gaactaagaa
     1441 accgcggttt ctctatcaat aaaaagaaag ttcagcgcct gatcaagaag ctaggaataa
     1501 aagtaacgac atacacacgt aaatcacggc gctacaattc ttatcgagga caagtcggag
     1561 cagtagccaa aaatcggatc catcgtcgtt tctacaccag tatctgccat cagaaaatca
     1621 cgacggatac aactgaattt aaataccatg aagtggatgc caaaggcatt atccgtcaaa
     1681 agaaattgta tctagatcct tttatggata tgttcaatag tgagatttta tcctatcgta
     1741 tctctgaaaa gcccaatgca ttagcggtaa tggaaagttt ggagtaagct atccagacca
     1801 ctaacgattg tccttatcgt cgtacgtttc actcagatca aggttgggcg tatcaaatga
     1861 aagcttatag aaacaaattg aaggagtaca aaatctttca aagcctgtct cggaaaggga
     1921 actgcttaga taattcactt atggagaatt tttttggctt attaaaacaa gagatttttc
     1981 acgggaaagt ttataactgt ttcgaggaat taaaatcagc catcgatagc tatatctatt
     2041 actacaataa tgaacggata aaacagaaat taaactggca aagtcccgtc caatttcgta
     2101 aaacaaccgt aatagttgtg taaaaatatc attttagagc atgaaaaaag gagtggattt
     2161 ctccactcca aaaaagtcta acttttgggg gtcactacaa gttgacaact cctttttgtt
     2221 tacaaatcaa ttggatatct acaaattgta ttttctaacg aattttatag acattaaaaa
     2281 attaatttaa cgttaaatac ttcctccaga aagtattgag attggtaaaa tataatcctt
     2341 atttgtcttt tcgcctttaa tttttttcag aagaatatca acaataagac ttgcaatttc
     2401 atcaattggt tgtcttattg ttgtcaattg tgggaaaaat ttctcaataa aagaggtacc
     2461 atcgtatcca ataattttta aatcttctgg gatattaagt ttaagttgat gagcaatttt
     2521 catagtgaga atggctgtta aatcatcaga aacaaaaata ccatctggtt tgtgatttat
     2581 aatagttgac tttatttcca tttcacgacg gatagtagac agattattag gaattttgaa
     2641 aatttttcca tttggatttt gaaaagaaaa acctagtgca cgtaattctg tgggagaatc
     2701 cgtgttatca tttcctgtaa tcataatggt attttgacaa ccattttttt gtaaagtctt
     2761 agctgcaagt tttccccctt caaagttatc tgaagaaaca atggaaacat taggagctaa
     2821 atttctatca aaagcaacaa taggtgctct cacacgctca taatcagcaa ttccaagatt
     2881 atggcttgat gagattatgc catcaacttg gttggcttca agcatttcaa tgtattctct
     2941 ctctttagca ggatcatttt gactattgca aataatcgtt ttatatccat gtttaaataa
     3001 ctctatttcg agatactcaa tgagttcaga gtaaaagata ttactaatat taggaaaaat
     3061 aagaccaata agctgaggtg actttccttg aagtccacgt gctaaattat ttggtttata
     3121 accaagagtt ttcatagctt catgaacttt ttgttttgtt ttttctgata aatatccctt
     3181 attattaatg acgcgtgaaa cggttgttgg gctaactcca gctaattcag cgacatcagt
     3241 taattttgct accattttaa tattttaatt caaagtattt tccagtaggt tcgcctgatt
     3301 tgatttgaat accattttgg tgattttcag ggaatacacg actagtaaac actttttctc
     3361 ctttattaat aaaaatctca atgattgagt tatctacaaa aatgttagca gttgtttctt
     3421 tagtatcaat tgagcattca cgaatcgtac caaagtcagt ggcatattga atgcctgctt
     3481 tgctgcgatc taaaataatt tttccttctt tggtatctac aatcaagcta agtccatttc
     3541 ctttactatc agagaaaaga agtaactccg atttctgatt tggaggaaaa gttagttcaa
     3601 gctcataagt attatttgtt tctgcttttg ctgtaaattg ttcagaacta gcacgtaatg
     3661 atgtaatagc ttctactgga tattggtaaa gtttaccatc ttttattgaa agttctttta
     3721 caagactcat tgctccctga taatcatact tatcagttgg ataatcgata tcaggtagtc
     3781 caatccaact gacaatcaga gcacgaccat caggagtatt gaatccttga gtcgcatacg
     3841 cttcaaatcc ataatcaaga ttttgaattt ctgaagcgcc cacaagtttt actttttcta
     3901 tgtcaagcga ttgacagatc ttaaaagtat tcggatagat attcccataa tttagctcag
     3961 atttatccaa cccttgagga caataaatga gaacaggttt tttgtctaca aatactagat
     4021 ttggacactc aatcatgtat tctgaaccag tgccgccaaa atcaaggttg ccaacttcct
     4081 cccatgtttc cacgttattg tcatccgctt tgtaaagttt gataaagcct gatttttcta
     4141 aattttgtgc tccaacaatc gcatacaatt gtccttcata attaaacatt tgtggatcac
     4201 gaaaatgatc agttacatca gcaggtttat gaataagaac attctcacct ttcgtaatgt
     4261 tgtagtcttt atccatccat gctccgattt gaagtgtatc acgttcccaa tttttatttc
     4321 gaacgtttcc tgtatagagt aagaatagat tcccctctat ttcatatgca gaaccggaat
     4381 aagcgccgtg gctatctcgt tttgtgtctg gaagaagttc tgctcctgtt acatgaaaat
     4441 gaactaaatc atctgattca ttgtgtaccc attctttcaa cccatgagca gctccaaatg
     4501 gccaattttg ataaaaaagc gtatattttc cgttaaaaaa agaaaaacca ttaggatcgt
     4561 ttaacaatcc tgttttaggt tcgatatgat agctagcacg ccaaggtgat cgttttacat
     4621 tatccttaat tttatccatc tcatctttag tccaatcaga ataagcacga tatcgtattt
     4681 ctcgaggtaa gttcatattt tactccttca atttatcttt ttacaactag tatggtaaac
     4741 gttttccgtt gaaaaatcaa tcatttcatt taaaaaaata aactatatga caaacgtttg
     4801 acatattttt aagatatgct aaaataaatt tgcatttcag tgaaccgttt tcacgtaaaa
     4861 ataaaaaagg agtgattcga atgaattaca gtaaagtagc ttccgacgta atacaagcgg
     4921 tcggaaaaga taaccttatt gcagctgcac actgcgctac acgtcttcgt ctcgtactaa
     4981 aagatgacac gaaagtgaat caaaaagctt tagacgaaaa tccagatgtg aaaagcacat
     5041 ttaaaattga tggacaatat caagtgatta tcggtgcagg tgatgttaat ttcgtttatg
     5101 acgaacttat caaaaaaact ggactttcgg aaatttcaac agatgattta aaacaaattg
     5161 ctagtaaaga taaaaaattc aaccccatta tggcattaat taaattatta tcagatattt
     5221 ttgttccaat tatacctgcc cttgttgcag gtggtttatt aatggcatta caaaactttt
     5281 taacagctgc tggacttttt ggaccaaaat caattgaaga aatgtatcct gcgattgaag
     5341 gtatttcctc aatgatccaa ctgatgtccg ctgcaccatt catgttctta ccaattttag
     5401 taggtatttc agctgctaaa cgttttggag caaaccaatt tcttggtgct gcaattggta
     5461 tgatcatgac aacaccagat cttggtggct caactgaata ttggaatatt tttggttatc
     5521 atgtttcaca aacaaactat gcttatcaag ttattccagt tttagcagct gtttggatat
     5581 tatctatatt agaaaaattc tttcataaaa aattgccatc atctattgac tttacattca
     5641 cgccattatt atcagtaatg attactggat ttttgacttt cacagttatt ggccctgtaa
     5701 tgttattgtt gtctaatgga attacagaca ttattgtttg gctatataac actactggat
     5761 ttattggaat gggtattttt ggaggaacat attcacttat agtcatgact ggtttacatc
     5821 aatcatttcc agctattgaa actcaattac tttcagcgtg gacaaatggt atcggacatg
     5881 gagactttat ctttgtagtt gcatctatgg caaacgttgc tcaaggtgct gcaacatttg
     5941 ccatttggtt cttaactaaa aattctaaga caaaaagtct tgcttcctct gcgggtgtct
     6001 cagcacttct tggaattaca gagcctgcct tgttcggagt taacttaaaa taccgctttc
     6061 cattcttttg tgctttaatt ggttcaggaa ttgctacagc agtagcagga ttacttaaag
     6121 tagttgctgt ttcacttggt tcagcaggtt tcttgggatt tctttcaatt aatgcaacat
     6181 ctattccatt ttacgtattg tgtgaattga ttagctttgt agttgctttt gcaattactt
     6241 acttctacgg taaaacaaaa gctgctggta tctttaaagc agaagaaatt gctgaacaaa
     6301 caccagttaa ttcttcaaaa gtagcaacaa cagatccaat atctactgat gttgaatcaa
     6361 cacaaacaaa agtttcatca gaaacaattg ttagtcccat ttctggtaaa gtgattagtc
     6421 tttcttcagt aaatgatcca gtcttttcat cagaagctat gggaaaaggg attgccatta
     6481 aaccaaatgg aaacacggtc cattctccag tagatggtac tattcaactc acgtttgaaa
     6541 ctggacatgc ttatggtatt aaatcaaata atggtgctga agttctaatt catattggta
     6601 ttgatactgt atcaatggga ggaaaaggat ttacacaaaa agttactact aatcaaaaag
     6661 ttagaaaagg cgatattctt ggagcatttg accgttcaat gattgagaaa gaaggtcttg
     6721 atgatacaac aatgattatt gtgaccaata cttcggatta ttcagaagta aatatagtag
     6781 ctgaaaatac agtcactgag ggtactgatt tacttgaaat caaataaata tatttccctg
     6841 agtaaagatg gaattttctt tccaaaatca aataatatag attaatcaaa tatttgagaa
     6901 taacatttta tttaatcttt tcatatatca attgtatgaa aaattattag aattttaaaa
     6961 aatgataaaa attcaaaaag aaaaaatagt agttatttac ttgtcatgtt actaaataaa
     7021 aaaataattt aaatatgata tatagtcatt tatttagtga tttcattcta aaataaaggg
     7081 agaaatgtct atgctata
//
LOCUS       DAMEET010000108         6493 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.111, whole genome shotgun sequence.
ACCESSION   DAMEET010000108 DAMEET010000000
VERSION     DAMEET010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.111"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            41..778
                     /locus_tag="PB780_002318"
     CDS             41..778
                     /locus_tag="PB780_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDE0734342.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            792..1607
                     /locus_tag="PB780_002319"
     CDS             792..1607
                     /locus_tag="PB780_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDE0734343.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1620..2267
                     /locus_tag="PB780_002320"
     CDS             1620..2267
                     /locus_tag="PB780_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDE0734344.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2283..2939
                     /locus_tag="PB780_002321"
     CDS             2283..2939
                     /locus_tag="PB780_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDE0734345.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3101..3922
                     /gene="racE"
                     /locus_tag="PB780_002322"
     CDS             3101..3922
                     /gene="racE"
                     /locus_tag="PB780_002322"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDE0734346.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            3925..5280
                     /gene="rph"
                     /locus_tag="PB780_002323"
     CDS             3925..5280
                     /gene="rph"
                     /locus_tag="PB780_002323"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDE0734347.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5281..5799
                     /locus_tag="PB780_002324"
     CDS             5281..5799
                     /locus_tag="PB780_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDE0734348.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            5906..6406
                     /locus_tag="PB780_002325"
     CDS             5906..6406
                     /locus_tag="PB780_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDE0734349.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         2134 a         1091 c         1436 g         1832 t
ORIGIN      
        1 taaaagaaat cttactaaat aataatggga gtgtaattct atggcaatga ttaaatttga
       61 tcacgtggat aaatattatg gcaagttcca cgcattaaaa aatatcaatc tcgaattcga
      121 gaaaggagaa gttgtcgttg tcattggtcc ttctggttca ggaaagagca cgatgcttcg
      181 ctgtatcaat ggattagaga cgatctcttc aggtaagctg ctgattaatg atactgactt
      241 gcatgataaa aagacaaaac tgacagaagt ccgaaaaaac atcggcatgg ttttccaaca
      301 tttcaatttg tacccaaata aaactgttct ggaaaacatc aaattagcac caatcaaagt
      361 gttgaagcaa gaccaggcaa ctgcagtcaa aaacgcggag aaatttttga aaactgtcaa
      421 tatgttagat aagaaagatt cttatccttc catgttatcc gggggacaac agcaacgggt
      481 ggcaatcgcc agaggtttag cgatgaatcc tgagatgctg ctgtttgatg aacctacttc
      541 tgctttggat ccggaaatga ttggtgatgt attagacgtt atgaaaaaat tggctcatga
      601 cggtatgtca atgattgtcg tgacacatga aatgggcttt gccaaagaag tggcggatcg
      661 tgttatcttc atggctgatg gagaagtatt ggaagacagc agaaatgtga gagacttttt
      721 cgaaaatcca aatgaagtcc gagcacagca atttatcagt aaagttatca atcattgata
      781 ggaggaaaaa gatgcgcagg ttaaaaaaaa tacgcctgat tttcctttta ggcattgctt
      841 ttcttgcact agccggatgt aaaggaaatg ggttggcaaa tgaagatatt ttagagcgaa
      901 gcaaatctac caatgaaatc atctggggtg tcaagtatga tactcgatta tttggaatga
      961 tggatattga gagcagaact gtacaaggat ttgatgtaga tattgcaaaa gcaatcacta
     1021 aaaagatcct tggtgacaat ggaaaaacag aatttgttga agtcacttct aaaactcgga
     1081 ttccattatt gaaaaacgga aatatcgatg caattattgc aacgatgacg atcacagatg
     1141 aacgaaaaaa acaggttgac ttttcagatg tctatttcga tgctggccaa gctttgttgg
     1201 taaagaaagg aagccaaatc aaaagtgtag atgatttgaa tgcttctaca acggttctag
     1261 cagtcaaagg ttctacatcg gctgcgaata tccgacagca tgcaccagat gcaaaaattt
     1321 tagaattaga aaactatgca gaagcattta ctgctttaca atctggtcag ggagacgcga
     1381 tgactacgga taatgcgatt ttgttaggga ttgcagatga aaatccagag tatgagttag
     1441 ttggtggaac gtttactaat gagccttacg ggattgcgat caacaaaggg caagaaaact
     1501 tcttgaaagc tgttaaccaa gcgctagagg aaatgcatgc agatggcaca tacgataaaa
     1561 tctatcaaaa atggtttcca aatgaaactg aaggaaaagt agagtaaggg ggagaggcaa
     1621 tgagcgaatt gatacaaact tatggaccaa tctttgtcga cgggttcaag atcacattgt
     1681 tttcgagtgt cttagcactt gtgtttagtt tgattatcgg aacgttaatg gcaatcttcc
     1741 aattatcaaa gaataaagta gtgagcgcct tagcaaaggc atatgtagaa ttttttagaa
     1801 acattccatt attaatcatc gtgatgtttt tttatgttgt cgtaccgatt tattggttta
     1861 atctagatgg attccaagca ggtacgattg gattaacgat ctatacatct gcttttattg
     1921 ctgaaacagt acgttcaggg attcaaaccg taccaaaagg tcaaacagaa gcaggactat
     1981 cttctggttt tacctattca gaaatcatgc gttatattgt tttaccgcaa gcattcaaaa
     2041 tcgttatccc gccattaggc aaccaattta tcaacctcgt aaaaaactca tctattttag
     2101 caatcgtagc tggtttagat ttgatgtatc aaggagactt gatcgctagt gaaacattca
     2161 ataccttcga tacgtatatc atcgttgggt tattttactt agttatcacg ctaccgttat
     2221 cttacttaat ggtttatctt gagaaaaaat gggcggttcg ttcatagaag gaggggaaat
     2281 acatggattt tagcggcgct ttttcatgga tgaatatccg ttttttattg gaaggattaa
     2341 aggttactgt tgaagtttca ttgttttcga tcatttttag tttcttgatc ggtggtctga
     2401 caggtgtgat tcgttttgcg aatatccctt tcttttccaa aatccttggt gtagtgattg
     2461 atattatacg gaatcttcca ttattattga tcattttctt cacttacttt gccttacctc
     2521 aaatcggcat tcagatgaac atcttctggt ccgctgtcgc agcattaacg atttttgagt
     2581 cagctatgtt gtcggaaatt ttccgagctg gactcaatgc tgttccaaaa ggacagatgg
     2641 aagcaggatt atcaacggga ctgacttatg tagaatcgat gcgaacgatc gtgttaccgc
     2701 aagcatttaa atcaatgatc ccggcaattg tgagccaatt gatttcactg atcaaagata
     2761 cttcattagc agtaatcatt tctttacctg aactttctca tcaggcacgt attatttatg
     2821 gacagaacac caattatgtg cttccgatgt tcatcatcat gacacttatg tacttcgtgg
     2881 tatgttacgc gctttctctg ctttccggtt acttggaaaa gagacggtac agttattaat
     2941 agatgattct tttagcggtt gtggcaaact aaagtcacaa ccgcttttcc tctttccatt
     3001 cgaaaaaaag agatttcgat tgttttaagc aagtaagagc acttgaaaat tcacgctaaa
     3061 aagtacacaa tagggaggat agaatttatt gaaaaaaagg atgatacgat tgacagataa
     3121 tcgccctatc ggatttattg attcaggtgt cggcggcttg actgtagtaa aagaagccct
     3181 gaaacaatta ccgaatgaaa atattttatt tgtaggagac acagcacgct gcccatatgg
     3241 ccctagaccc gcggaacagg taatccagta tacttgggaa atgacggatt atctggtgga
     3301 gcaaggaatc aagatgctgg tgatcgcctg caataccgca actgcggtgg ctttagaaga
     3361 aatcaaagct gctctttcta ttccagtcat cggtgtgatc cttcccggta ctagagcggc
     3421 agtaaaaaaa acacaaaata aacaagttgg cattatcggt acgattggta cggtaaaaag
     3481 tcaagcttat gaaaaagcac tgaaagagaa agtaccagaa ttgactgtga caagtcttgc
     3541 ttgtcccaaa tttgtttcag ttgtcgaaag taatgaatac cattcatcgg tggcgaaaaa
     3601 aattgtggca gaaacattag ctcctttaac cactaaaaaa atcgatacat tgattttggg
     3661 atgcacccat tatccattat tacgccccat cattcaaaat gtaatgggag aaaatgttca
     3721 actgatcgat tctggagcag aaacagtagg tgaagtatct atgctgttag attatttcaa
     3781 tttgagcaat tcaccgcaaa atggtcggac attatgccag ttttatacaa ctggctctgc
     3841 caaacttttc gaggaaatag ctgaagactg gcttggaatc ggacacttaa atgtagaaca
     3901 tatcgaattg ggaggaaaat aaggatgcgt catgacggga gaaaagccca agagcttcgc
     3961 aaactggagc ttcaaacaaa tgttttcaaa tatccagaag gatcggtagt gatccgtttt
     4021 ggtgatacaa cagttatttg ttctgcaaca atagaaaatt ctgttccccc ttttttgaga
     4081 gaaacaggaa caggatgggt gacagcagaa tatagtatgc tgcctcgagc aacgagtacg
     4141 agaaatcgta gagaaagcag caaagggaaa ctttctggta gaacaatgga aatccagcgt
     4201 cttattggtc gttccttgcg tgcagtagta gacttggaaa aattaggaga acgtagtatc
     4261 atcgtagact gcgatgtgat ccaagcggat ggcggtacta gaacagcaag tatcactggg
     4321 gcatttgtgg cattgaaatt agccattgaa aaacttttaa gagaaaaaga attgtcagaa
     4381 gatccaataa aagaacatct tgctgcagtt agcgtaggaa tcttaccaga tgggacttgc
     4441 gtcacagatt tggattacca agaagattcc gcagcacttg tagacatgaa tcttgtaatg
     4501 acagaatctg gaaaattcgt ggagattcaa ggaactggag aagaagcaac gtttgatggt
     4561 gaacagttga atgaaatgct cttttttgga aaaaacgcga ttgaagattt gatcaaagag
     4621 caaaaacatg ctttgctaac agagtttgct caaaatgatg agcggataga agaaacaaaa
     4681 acaatcatca ttgcgacaag aaatccagga aaagcagaag agtttcgaaa tatgttcaaa
     4741 gaagcgggat atcatgtaaa aacattactt gactatcctg aattgccaga tgtggaagaa
     4801 actggcagca catttgaaga aaatgcccga ttaaaagcag aaacgattgc acaattgctt
     4861 gatcagccag tcttagcaga tgattcaggt ttgaaagtgg atgctttagg cggtatgcca
     4921 ggaatctatt ctgcacgttt tgcaggggaa caaaaaagtg atgcggggaa taacgccaaa
     4981 ttattatatg aattgacaga tgtgccggat gaaaaaagaa cagcacaatt ccattgtacg
     5041 ttagtattcg cggcacccaa aaaagacagc cttgtagtag aagcggaatg gcccggtcga
     5101 gtagcccgta taccatctgg cgaaaatggc tttgggtatg atcctttatt tatcccagaa
     5161 ggtaagaaac aaacagcagc agaattatcg agcgaagaaa aaaacaagat cagtcatcgg
     5221 gctcaagcaa tgaaaaaatt gagtgccgag tggaaacaat ggctggaagg agagagatag
     5281 atgcggtatt tagtagtaag tgataatcat ggagacagag atatcctcgt tgacttagta
     5341 gatcgttatc gttctgaagt ggatatcttt ttccattgtg gtgattcgga attagaagct
     5401 tctgacccat tatgggatac attccaagtg gttcaaggta attgtgatta tggaccagga
     5461 ttcgaacaga aaaaagtgat ccaaactggt caggatacag tattcatgac tcacggtcat
     5521 ttatcgaatg tccgatttgg tttgacacag ttagcaatcg aagctcgagc tgcaactgcc
     5581 gatatggtgt tttttggtca tacccatcaa ataggctgtg agatggatca gaaaatcctg
     5641 tttttgaatc ctggtagcat ttctcagcct agaggcccga tccaaatccc atcatatgct
     5701 cttatcgaaa gtttagatga ccaaatttct gttcaatact ataaccgtgc ccatcaggca
     5761 gttgaagagt tggccttttc tttttcgaga gagcgctgaa taaaaaaact ctaattttta
     5821 ataaaaataa gctttatttt atgaattttg catccggttt cggtaaaata gtagtgttga
     5881 atgaaataga aatggaggcc atcagatgat tggacctata gtaagagaaa tgctgctgga
     5941 aaatcaggaa acgtttttga ttcctgcaga aaatgtggca aatgttatgt atcatcatcc
     6001 attgtcccat ggtttacttg tgttatcgaa ggtcggttat tccaaaattc ctgtattagg
     6061 aaaagatgat cactttgtag gactagtaag tttatcagat gtggtcaata aaatgattga
     6121 cttgcaaaca atcagcatgg acccgttaga aggactaacg gttgctgatg tgacagaaac
     6181 agatgttccg acaattgatg aaaattggga actagaagaa gtactccatt tgttagtcga
     6241 ttcgcctttt ttaccggttg tcacagaaga tcggattttc aaaggaatca ttacaagaaa
     6301 agagctgctt aaagccgtaa acttcatggt gcatgaatta gaacgtcgta atatattgtc
     6361 tccgaaaatc gaagaattaa aagaacaaat cgatatcgtg agctaaagaa cgagaaacta
     6421 aaagataatc aaaaaataag tagaaaaaac agcgtaccag ttttgacttt atcttgaaaa
     6481 agaggctggg aca
//
LOCUS       DAMEET010000109         6313 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.112, whole genome shotgun sequence.
ACCESSION   DAMEET010000109 DAMEET010000000
VERSION     DAMEET010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.112"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(41..493)
                     /locus_tag="PB780_002326"
     CDS             complement(41..493)
                     /locus_tag="PB780_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDE0734350.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLMQTDISLVS"
     gene            complement(958..1326)
                     /locus_tag="PB780_002327"
     CDS             complement(958..1326)
                     /locus_tag="PB780_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HDE0734351.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            1476..1958
                     /locus_tag="PB780_002328"
     CDS             1476..1958
                     /locus_tag="PB780_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HDE0734352.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSEHMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLLNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1995..3005
                     /locus_tag="PB780_002329"
     CDS             1995..3005
                     /locus_tag="PB780_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HDE0734353.1"
                     /translation="MISYPLLERNTTIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGINLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(3120..3833)
                     /locus_tag="PB780_002330"
     CDS             complement(3120..3833)
                     /locus_tag="PB780_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734354.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSSDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPTHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
     gene            complement(4023..4613)
                     /locus_tag="PB780_002331"
     CDS             complement(4023..4613)
                     /locus_tag="PB780_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HDE0734355.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            5136..5807
                     /locus_tag="PB780_002332"
     CDS             5136..5807
                     /locus_tag="PB780_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDE0734356.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLAENYDNHVVIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGY
                     SFILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEM
                     FINAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPNTIYFKRV
                     GGGIT"
     gene            5804..5965
                     /locus_tag="PB780_002333"
     CDS             5804..5965
                     /locus_tag="PB780_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734357.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMKYVADEANRK
                     QRKLVGLDK"
BASE COUNT         2153 a         1002 c         1140 g         2018 t
ORIGIN      
        1 atttccaaca tctctggaat ttctagcgct ttttaattgt ttaagataca agtgaaatat
       61 ctgtctgcat caataatttt tcattgatcc attgactaaa gtttacgaca taattgaagt
      121 taccaccgat tagtatctcc gcatgattgt ctttataaag atcgtattgt aaaagacggt
      181 cttttttatt tgcgtaaaca tcgttatggt actggactaa ttgttctcgg aattgattga
      241 ttgctgtaaa atcacgatac tgaaggttga aacgcatcgt gattggtgca tttagcatga
      301 atcgctgcat ttcttttgct gcttcctctg cttgatctcc gtataggaac tcaaaaagat
      361 tacgttcaga aaatttccat tgcccgccaa cctttgttcc ttttagtttt ccttcatgaa
      421 gataatttct gatagaacgt gtagtaacac ctaattgttc ggctaattca tttactgagt
      481 aaaaatcttt caatgttcac cctcctatgt gaatggttct actgacttag ttgatatttg
      541 cttaatcacg aaatgtagaa agaacgattt taattaaggt cattgtaaaa atcaagtcag
      601 aaaattctaa cttttctttc tctaaagaaa ttcgttctct cctaaagtga tattatattt
      661 aaatatacct gaaaataatg ggcagtatta tgaaagatca ctgatatttt ctcgtatttt
      721 ttccttattc aaaagttttg caggaaaatt gctgaaaaaa taattttact aagtggaaaa
      781 ttcaaagcca ttcaatcagt gatttttttc agcacaagaa agactcctta taaaaatagc
      841 agatactttg tcaattaagt gtttaaccag ggatgataaa aaggatagga ttgaacaaaa
      901 cctgtataag ctgatgaata gtttcctcgc cagtaaaagg tttattcgta taaagcttta
      961 tcgccaaaat ttatcatgac cttttttatc ttcaatatta aatgaaatca attgttcaat
     1021 caattcccag ttgattacac ttttccaagg aactttgatg acgttatctg tatatacgta
     1081 gcctgcttct tggatatttt tttcaagggc acgaatcgct gctttttccg gtgcgataga
     1141 aaaatgattt ttagcaaaac taaatgcaat aataaaggta ccatggtcag taaacatagg
     1201 ttggttccat tttacagttg tgtctagctg gggatacgtt tctgttatct tagcaaaaag
     1261 gtctttgatc ttttgttgat gttctttttc tttgatctga ttgatgatcg gctgaaattt
     1321 ttccattcta aaacgcctcc attaagtggt ttgatttatt ttaccagaaa aacaatagtt
     1381 atgtataaaa aacgaacaaa acacaagtaa tgttgtaacc gctatattta tatgataaga
     1441 tgtctcaaat aataaaatag aaaagagaca taaaaatgat tgacttggca aagaaattct
     1501 tacacgaaaa tatatatttt gagttgtatc atcatcgagc catttataca aatgaagatg
     1561 ctttggtaat aaagaaagaa caaggattta caggtacaga gaccaaaagc ttattcttaa
     1621 aagataaaaa agataataac tacgtatatc tgacttttac tacgaaacaa acggatttca
     1681 aagcattggg gaagatcgtc ggtaaaaggc tttctattgt ttcatcagaa cacatggaag
     1741 aacaaacggg acaaaagcag ggcgctgttt ctccgtttgg ttatgaaaaa cagattccga
     1801 ttattattga tgaagagctc ttattaaatg aaaaacttgt ttttgcacca ggtagaccag
     1861 atcaaacgat ggtcatccaa acggttgact taaacaaaat catccagcta cttgagatgc
     1921 cgatattttt gatgccggca aacgaaaaaa acaattaacc aagtaaaata gtgcatactt
     1981 ggaggtgaag caacatgata agttatccat tattagaaag aaatacaacg attggggtta
     2041 ctgcaccatc ctcaggcgta tcagaggaac accattcgtt actcgaatca gctttaaaga
     2101 gaatggaaga aaaatctttc caagtagtta ctggaaagac gctttggacg caagataagg
     2161 aaaaatcagg tccagcaaga aaacgagcag atgaattcaa tcaaatgatg caagatgaaa
     2221 atatccaaat gatctttccg ccttggggag gagaattatt gattgagatt ctcgattttg
     2281 tcgattttga tgctctacaa ccaaaatgga tagtaggtta ttcagatatt agtacgttgc
     2341 tacttgctgt cacattaaaa acagggatag ccactgcaca tggaatcaac cttattgatt
     2401 taagaggaga gtacttggat gaaacgacag caatgtggga aagtgtactg aaaacaagag
     2461 aaggagaatc gattctacag caaccttctg aaaagtatca aaaagagtgg ccccatgagc
     2521 attcatcatc ttctgttttc catctcactg aaccaacaaa ttggcaatca gtcagcggag
     2581 atgcagtaag gatgaaggga cgattattag gtggctgtat tgatacgatc agtcatttaa
     2641 tcggtacccc ttatggagat gtccaatctt ttaaaagaaa gtataatgat ggaaagccga
     2701 tcttgtggtt tctagaaaat tgtgagctat cgtcagttga tttaagaaga tcacttgttc
     2761 agatgaaact ggcaggatgg tttgatggca gctcggggat catgttcggt agaagtgcag
     2821 cagaggattt gttagattat aaaatgaagg atgtatacga agatctgtca aaggaattaa
     2881 aactaccgat cttgtatgat attgattgtg gtcatgtacc gccacaactg acattgatca
     2941 atggtgccta tgcagaagta gcggtagaag atagaaaagc gactgttttg caaacgttta
     3001 tttaaaaaat tttttcctgc cttggagttt atcaaagacc aacaaatcta aatagaaaag
     3061 aaaatgaaag aagctgacag tattttgcca gcttcttttt gtatgatcta ttagaaattt
     3121 taattatcca taactatcac attgatcagc tgtttcacat catttggatt acctgtagga
     3181 atagctacta cagatgcatt atcaataatt tcttgagttg tttctctaat gaacgctgga
     3241 taagatgttt gatctgcagg catttgccaa tgtgtaggaa ctggataact agtgattgtc
     3301 ccgtcatggt tacttgaata cactaaaact ccctggtaac tgtatttccc agtgagcata
     3361 accacatctt tcggatagac tgcactccct gcatcataag gggcgatagg tgttcctgca
     3421 ggataatgaa taacatgtag ttcgaaatca ctcgatgtat ctctaaaagt attacctcta
     3481 atagctagcc aaacacgtgc gtactcaatt tgtagatcag aatatcctgc taacggatct
     3541 attgactgag aagactgtga ggaagttgtg tctattgttg aagaagaaat atttgtctca
     3601 actgtactac tagatgtaga agaagtattg ggctctgaga ctttactgct tgttgaagat
     3661 gtattcgttt cagtagaagc tgatttctga gtacttgatt tttcagctga actggtttgt
     3721 ctgattgaac ttttgtctgt ggtcgcagat ggatcactat tttctttact aggagacgta
     3781 catcctacaa ggaataaaga gaccaacaat aaaataaaac ctaacttttt catataaaat
     3841 acctcattat ttttttaata ccagtatagc gtaatcctga atttttgaat ccgtaaaacc
     3901 ttgtctatga atataaatag agtatatctt ctttgataaa tgtcagaaaa agtgtgacaa
     3961 ataaccatca gtaaaattac ttgtcatact attgagaagg agtatttaat tgaagagaat
     4021 gattaggaga aaggatctcc gtacttttta tagtaatcat agaatccaag tttgtataga
     4081 cgaatcgttt tttctcttat ttcttctgtt tcaaaatgga ctgcttgaag aatcaagtct
     4141 gtgaattcta taggagctgt tccattcgca gtaatcaaat tacgatcttt cacagcttgt
     4201 tgttctataa aatcttcttt attctgataa ttttccgatt ctaaccaagg aaattgcgca
     4261 tttccggtgt gtttgtaacc ttgcaataat ccgctctgag ctaaataatc aactgcccca
     4321 caaatagcag ctactggtac gtctgttttt aacaatttat cgattatttg atataaatga
     4381 tgatttttta tgtcccatga attgccaccg atcataataa aaaggtcaac tttttcaggt
     4441 atttgttcga ttaagtaatc gatttttgtt tttaatcctc caatggaagt gacctctgat
     4501 tggatagaag cagttttgtt ttgccatccc tctttttggt ttagtgtgct agtaatataa
     4561 gcaccttccc aatcggcata ttcctctaat agaaacgtaa tcgcagtttt cattttcaag
     4621 ctctccttta ctatctatga caaggacaac agttttaaca tacagacatt cattaaattt
     4681 ggatagcgac agcgaaaaca acaattctta agttttagac agagtataca gacagtcaac
     4741 ttaattaaat acgaactaaa taaaaatgac aggtaatcat ttttgtttac cagcattata
     4801 acattttgca tacatggatg acaaaagaac caattcccac ttgcttgagt acaatcttaa
     4861 aagaaactgg aaggcaaaaa gtaattatag aaacgaaaaa taaatcatat cgggcactca
     4921 gacatgaaaa caaccctaga aatatataac tagtccaatg aaaaaacagc gaatgccgta
     4981 gaaaacaagg attctgcgta cattcatatc tcattttgcg agcatatctg ctattactgt
     5041 gactaaatgt taatatggtt aaaatgaccg ttatattttt tatataaata tcgtataatt
     5101 agtatgaata tagaagaagg aaagaaggtg caatcttgga acctaaagac aagtatttag
     5161 attacgctag ggtcaataaa gataaattta ttgaaacaat catcaaggga aaaatccctg
     5221 atgatgaaaa agatgcggta ttcatggctg gaagcccagg agcaggtaaa acagaggttg
     5281 cactaggttt agctgaaaat tatgacaacc atgttgtgat tgatgcggac tattttagag
     5341 cacaatttcc agaatacaat ggaaagaact caagtgtttt tcagaaagct tcttcttggt
     5401 tagttgaaca atcattaaaa tatgtactgg aacatggcta ttctttcata ctagaggata
     5461 cttttgctat ccttagtgca gagaaaaata ttattcgagc ccttaaaaat aactttcgcg
     5521 ttacgatttt ctatgtttac caagatccca aagttgcctg ggatttcact cgaaaacgtg
     5581 aactagctga aggacgtcat gtgccaaaag aaatgtttat caatgcgttt tttaagtcca
     5641 gagagaatat tgaaaaagtc aaaaatagac atcctgaagt agtccttcac ataatgatta
     5701 aggattatca aaacgacatt tctgaagtgc attatgcagc agataatatt caattagtac
     5761 tacccaatac aatatacttc aaaagagttg gaggaggaat tacatgactg agaaagaaca
     5821 agtacaacaa atcgtgaaga agtataataa aagtatcgct gatttatcag aaaacgcctc
     5881 agcgaaagaa tttaaaacag taatgaagta cgtagcagat gaagcaaatc gtaagcagcg
     5941 aaaactagtt gggttggata aataaatttt ttaaaccata tccttgtaaa gttgagtttt
     6001 ttgttttaat gaattaagac tattaaatag ataattattg taacttaaaa aacacagcta
     6061 aaaattagtt gtgtttttta ttaggtttac ttttctggta taaaaggaac tagctacttt
     6121 ttcaaaattt ctaaatcatt caatatgtag cgcttactaa attattcgcc ataaaatttt
     6181 ataattggat cactcattgt tttacctaag tcatatttaa gtaataaccg gtgtttttac
     6241 ataagaagac ttggggtatt ttttagtttg attagctctg atatacctat aaagaatatg
     6301 ctaaggtaag aat
//
LOCUS       DAMEET010000110         6148 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.113, whole genome shotgun sequence.
ACCESSION   DAMEET010000110 DAMEET010000000
VERSION     DAMEET010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.113"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            103..2544
                     /locus_tag="PB780_002334"
     CDS             103..2544
                     /locus_tag="PB780_002334"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HDE0734358.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKPYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     KVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2874..3578)
                     /locus_tag="PB780_002335"
     CDS             complement(2874..3578)
                     /locus_tag="PB780_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734359.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3765..4010)
                     /locus_tag="PB780_002336"
     CDS             complement(3765..4010)
                     /locus_tag="PB780_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734360.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4027..4878)
                     /locus_tag="PB780_002337"
     CDS             complement(4027..4878)
                     /locus_tag="PB780_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDE0734361.1"
                     /translation="MNSLTSTYRLLNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQPTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5044..6048
                     /locus_tag="PB780_002338"
     CDS             5044..6048
                     /locus_tag="PB780_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDE0734362.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1974 a         1065 c         1299 g         1810 t
ORIGIN      
        1 atttaaggat tgttaaaatt taatcttaga aaaaaacaac tggaacaaga gctatttgtt
       61 tttacagcaa agaatggtat atttttgtag gaggtggctt acttgaaaga agtcgttatg
      121 atagatgctg caagaacgcc gataggaaag tatcgcggaa gtttaagtcc atttactgca
      181 gttgaattag gcacacttgt gactaaagga ttattagata aaacaaaact gaaaaaagat
      241 aaaatcgatc aagtaatatt cggaaatgtc ctgcaagctg gtaatggtca aaatgttgcc
      301 agacagattg ctttgaacag cggtctccct gtagatgtcc cagcaatgac gatcaatgaa
      361 gtttgcggat ctggtatgaa agcagtgatc ttagcacgcc agctgatcca attaggtgaa
      421 gcagaacttg tgatagccgg cggaacagag agtatgtctc aagcacctat gctgaaaccg
      481 tatcagtcag aaacaaatga atatggtgaa ccaatttcca gtatggtcaa cgacggattg
      541 actgacgcat tttcaaatgc acatatggga ttaaccgcag agaaggttgc aacacaattt
      601 tctgtgagca gagaagaaca ggatcgctat gccttgtcgt cccagttgaa agcagcacat
      661 gctgtcgaag ccggtgtatt ttctgaggag atcatcccag tcaagatttc tgatgaagac
      721 gtgttatctg aggatgaagc agttcgtgga aatagtacat tggaaaaact gggcacgtta
      781 cgtacagtat tctcagaaga aggaactgta acagcaggaa atgcttcgcc attgaatgac
      841 ggtgcctctg tggtgatcct tgcatccaaa gaatacgcag aaaataataa tctgccttat
      901 ttagcaacca tcaaagaagt agcggaggtc ggtattgacc caagtatcat gggaatcgct
      961 ccaatcaaag ccattcaaaa attgacagat agatcaggaa tgaatttatc aacaatcgat
     1021 ctttttgaga tcaatgaagc atttgcagct tcttctatcg tagttagcca agaactgcag
     1081 ttagatgaag aaaaggtcaa tatatatgga ggagcaatcg cattaggcca tccaattggt
     1141 gcaagcggtg cacgtatctt gactacatta gcttacggat tgcttcgtga acagaagaga
     1201 tacggtattg cttcattatg tattggtgga ggtttaggct tggcagtctt gttagaagcg
     1261 aatatggagc aaacacataa ggatgtgcaa aaaaaaaagt tttatcagct tacgccatct
     1321 gaacggcgca gtcaattaat agaaaaaaat gtattgacac aagaaactgc gttgattttt
     1381 caggaacaaa cactttctga agagctctcc gatcacatga tcgaaaacca agtgagtgaa
     1441 gtggaaatcc caatgggcat cgcgcagaat tttcaaatca atggtaagaa aaaatggatt
     1501 ccgatggcaa cagaggaacc atctgtcatt gcagctgcaa gtaatggtgc aaaaatctgt
     1561 ggaaatatct gcgcagaaac acctcagcgg ctgatgcgag ggcaaatcgt tctatcagga
     1621 aaatcggagt atcaagcagt gatcaatgca gttaatcatc gaaaagaaga gctgattctt
     1681 tgtgcaaatg aaagttatcc ttcaatcgtg aaacgcgggg gcggtgttca agatatctct
     1741 actcgggaat tcatggggag tttccatgca tacttatcta ttgatttttt agtggatgta
     1801 aaagatgcaa tgggcgctaa catgatcaac tcgattcttg agagtgtggc aaacaagtta
     1861 cgggagtggt tccctgaaga agagattcta ttcagtattt taagtaattt tgctacagaa
     1921 tcattggctt ctgcctgttg tgaaatccca ttcgaacgct tagggagaaa caaagaaatc
     1981 ggtgaacaaa ttgccaaaaa aatccaacag gcaggggaat atgcgaagct tgacccttac
     2041 cgtgcagcaa cgcacaacaa agggatcatg aatggtatag aagctgtagt ggcagctaca
     2101 ggaaatgata cacgtgcggt tagcgcttcg attcacgcct acgcggcaag aaatggatta
     2161 taccaaggat taacagactg gcagatcaaa ggggataaac tagttggaaa actaacggtg
     2221 cctttggctg ttgccacagt aggcggagct tccaatatcc tgccaaaagc aaaagcatct
     2281 ttagctatgt tagatattga ttcagccaag gaactagcac aagtaattgc tgcagtaggg
     2341 ttagctcaaa acctggctgc tttgcgtgca ttagttacag aaggaatcca aaaaggccac
     2401 atgggacttc aagctcgttc attagcgatc tcaattgggg ctattggtga ggaaatcgag
     2461 caagttgcga aaaaattacg tgaagcagaa aagatgaacc agcagacagc tatacaaata
     2521 ttggaaaaaa tccgggaaaa ataaaaaagc aatatcttta gaagaataaa gcaagattta
     2581 cagttatttt atttactgca aatcttgctt ttttctctat cagaaaatat gtttattatt
     2641 agaatttttt atagttaagg ttaggcatct taagaaatca ggcaactcca agatatcttc
     2701 aggtagaaga gaagtcgcct aacattgtag gatcataggt gatttgttgg acaggtcaag
     2761 ctgatatttg agcctgatgt tagaaggaat ttctttttac ttgctgtaga aattattata
     2821 tgactaaaaa ggctgggatt ttaaatccca gccttttatg acaaatagaa tttttatcga
     2881 tcagtaatcg ctgtcgctgc aaattgttcg atcaaaggaa tcaaagtcaa tttgactttg
     2941 tatttccgtt cgagtgcttt ttcgataacg aactcatttt cagtagtcat agaaatagtt
     3001 gctaatttac gaggttcatc agcatttttt tcattaataa ctacttcatg ttcattttgt
     3061 tctgtgtaaa atttaggata ctcagcaacg gcttttgaaa aatcagcaaa acttaaagcg
     3121 gcattcgtat cagttgagcg tgtattttca gttgtgtccg cttttggttt ttccgttttt
     3181 tcttctggca cactgactgt ttcttctgta actggtgtcg caacagcttg cgcagttggt
     3241 tcatcgtttt tcaatcgttg atcattccag taagacacga tttcaataag aagattgtca
     3301 cggaatcgtt cgaaaatccc gttttctcct gaacgaattc tctttaatac agtattgaag
     3361 ttgtcatcga ttggcacgtt gatccgtgta tcttcatttg ttttcccagc taatccgatc
     3421 gttaagattt gacggttttt gggatcaagc atgacttgta acggcaaacc atagcgtcgc
     3481 aagctctctt cttctagaga acggaataat tgtcctctta caccatccaa tgaaggacgg
     3541 aattgattta ataatttttg tttctgtttt ttcttcatat gttctctcct tcgctcatac
     3601 cctttcgatt ataagaaaaa aacagccaaa tgaaaaggag taatgcaaaa aaacttgcgt
     3661 atacgcaaat tacaacagaa aaatttgcgt attttcccgt tttttgaaag aaaaataaaa
     3721 aatggaacgg attatgtata tatacataat ccgttccatt ctttctattt tttcggtatc
     3781 aaggcaaaaa caggtagttc aggtgaataa atgacttcat gttcgtgcca gggtttgacc
     3841 attagcgaat gatcgaaccg ttttttcagt tcttcgatga tttgatcatg tgtccagttt
     3901 cttgcaggaa aatgttggat tttctctgca tcgatcaaaa agacaaaatc ggaatgaaaa
     3961 gtctgggtaa cttcttccga gagagaattc aagcgttcga ttggatccat aaaatcacct
     4021 ttcgacttaa aattgggtag tatctgggtc ttttgctttg ccgactacac catctaactg
     4081 atcgatttgt ttcatttctt cttctgttaa ttcaaaatca aataattcag tattttcttt
     4141 gattctgctt ggtgtcactg acttaggaag aggaaggaat tcgtgttgca gcgaccaacg
     4201 caaagcaact tgtgcaatcg tcttgtcatg tgcttcagcg atttgtttca tttcaggcac
     4261 atcgaagatt ttaccagtgc ctaatggact atatgcttcg aggatcatat catgtttttt
     4321 tgcatactca actacagttg gctgtaattc gcctggtgca aggaaaactt gattgaccat
     4381 cggcataatt tttgctgttt ttgctagtgt atctagatgg tggggtaaga aattgctcac
     4441 accgattgct ttgattttac ctgcttcata cagttcttca aatgctcgcc atgtatctgc
     4501 atttgcttgt tcccaattat cacgaaaagc aacagggttt ggccagtgga tcaaaaacag
     4561 atctaaataa tctgtttgga gcttcttcaa tgattcttca aaagaagaca tcacaagttc
     4621 atagctgtgg tttgcattcc ataattttgt tgtcaagaat atctcttctc taggaattcc
     4681 actttcctta atcgcttggc cgacactttc ttcattcttg tatccttggg cagtatcgat
     4741 atggcgataa cctgcagcca gcgcttcttt aacagaggaa acagcgacat ccccatcagg
     4801 tgtttgccat gtgccgaatc ctactaccgg gatttcgtaa ccatttaata gacgataagt
     4861 gcttgttaat gaattcatac tttcagctcc ttttttattc gttcaactat aagtgtagcg
     4921 catttttcga aaaaaagaat ccattttgtt tctactcttg aaatttttgt ttaaaagaaa
     4981 atgattttga ttctggtatt tttgattttt tttccgtaaa ctaagacaga agggaatgag
     5041 aaaatggata tctatttaaa aaaagcaatt ctgcatatta ttgacagaga aactggttct
     5101 ccagtctttt cacaaaaaga gctggatctg acaaaagaat atattcgtga ttttcttcaa
     5161 aagaagatcc aaaaaatctc ttctgctcaa acaaaaactg gacaactagc agaagaaagc
     5221 atgatcgttg aatcacttaa aatcgctcaa gaaaattttg tggaagcaag tgaagcaatc
     5281 gttcagcggt ggttcgatat ctaccaagaa agtgaagaag cacctagtgc agatgtattt
     5341 gttgttttgt atgaattaga tacagtgatg tacttatctt tattgaaagt caattatcgt
     5401 gaggcttaca ctcactttgt tgaagcagac gaagcaggga tcgacaaccg cttgattttg
     5461 aatcgagcaa ttctaggggc gaaaacacaa aaagcagatg aagcattcgc tgtgaatttg
     5521 actgattgga cctacgaatt aattgaaaaa aaatatgagt tttctggcaa aaaagagctt
     5581 tatttttctg gaagagtgat cgaaagtgtt cctgcacctt ctttagaaga aaatgtaaaa
     5641 gtgatccaaa aagtagccaa gcatcttgga aaaaaatttg agacagaaga atttgatatc
     5701 atggctgatt tgaaagaagc tgtctatgat acgatagaag aaaaaggtcg gttggatcat
     5761 gagatgattg cggaaaaagt cttcaaggaa aacatcacgg caaaaatggc ttttaaagaa
     5821 gaagtacaag agcaaggatt tgtcccagaa gcacctcctg taaaagaagt acaagaaatt
     5881 tctgagaaaa aatttggaaa acaaaaattc aagctatcaa acgggatcga actgatcgta
     5941 ccagtagatg tatatcgcaa cccagatttg attgaatttg tgaataatcc ggatggtacg
     6001 atatccgtga tgatcaaaaa tgtggatgaa gtattgaatc gtttataaaa atgactagaa
     6061 cattaggtgt tcaagtacca gtgatcagtt aaatgaattg tgtcaagaga aggatcggaa
     6121 aagacgaata taacaaaaaa agagcgtg
//
LOCUS       DAMEET010000111         6111 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.114, whole genome shotgun sequence.
ACCESSION   DAMEET010000111 DAMEET010000000
VERSION     DAMEET010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.114"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(322..807)
                     /locus_tag="PB780_002339"
     CDS             complement(322..807)
                     /locus_tag="PB780_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734363.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(946..1161)
                     /locus_tag="PB780_002340"
     CDS             complement(946..1161)
                     /locus_tag="PB780_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HDE0734364.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1385..2185
                     /locus_tag="PB780_002341"
     CDS             1385..2185
                     /locus_tag="PB780_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDE0734365.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2204..4072
                     /locus_tag="PB780_002342"
     CDS             2204..4072
                     /locus_tag="PB780_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDE0734366.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            4065..4556
                     /locus_tag="PB780_002343"
     CDS             4065..4556
                     /locus_tag="PB780_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDE0734367.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4544..5098
                     /gene="srlA"
                     /locus_tag="PB780_002344"
     CDS             4544..5098
                     /gene="srlA"
                     /locus_tag="PB780_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDE0734368.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            5116..6111
                     /locus_tag="PB780_002345"
     CDS             5116..6111
                     /locus_tag="PB780_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDE0734369.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2162 a          968 c         1224 g         1757 t
ORIGIN      
        1 tttgaaaaag ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa
       61 ttcttctatc caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt
      121 aactcagttg accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa
      181 gtgagtatat tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat
      241 ttatactccg tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta
      301 aatccgtata atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga
      361 tccttcgggt gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg
      421 tttgctggaa gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat
      481 atcgtcactt tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt
      541 ttctaatgga agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa
      601 cgactgaata cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat
      661 ggaagtagta gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga
      721 ttctttaaca gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa
      781 caagcctgcg taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc
      841 agtatatcac ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa
      901 gggaaggttc atgaagataa taactttttt aataattata gctttctaat atctttcatc
      961 aacttttcta gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac
     1021 gatataaatg atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc
     1081 aagttcgaac aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc
     1141 aatagcatct aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat
     1201 caaaaagaga aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat
     1261 gaaaattttt tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg
     1321 tcttaatttt ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc
     1381 acatatgtcg gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc
     1441 aggaattgga agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt
     1501 tgatgtaact gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc
     1561 ttctaaaacg gacatagaag aagggatata taaagtaatg aaacatttca agacgatcga
     1621 tggtctagtc aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc
     1681 aaaaagcaag tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa
     1741 aagtgtatat ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt
     1801 gatcgtgaat ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc
     1861 tgctacaaaa gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag
     1921 gaatgttcgt gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca
     1981 agaatatgaa gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg
     2041 ttacagtcga acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga
     2101 tctagtctgc tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat
     2161 ttcaggaggt aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag
     2221 atggtaccaa ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca
     2281 aaaagaaatc aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga
     2341 aattgccggt attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga
     2401 ttttgatttt tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc
     2461 ggcatccaaa agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg
     2521 tatggatgat ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa
     2581 gacattaaaa gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg
     2641 actaagaata ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc
     2701 ctacttttca gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt
     2761 tcagaagaag acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat
     2821 ttctttgaat agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg
     2881 gtgttttgaa tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga
     2941 actaactttg agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac
     3001 aagtataacg atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat
     3061 gatgcgtatt cgacgattag atctcattga tctggatgag tccttcttat atcaagagat
     3121 gaaacagcac ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt
     3181 attttatggt gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat
     3241 acaagcatta gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac
     3301 gttgtatttg gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc
     3361 cattgttcat acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat
     3421 attggggagg gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct
     3481 aaacaaatat ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc
     3541 ggtgatccat ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc
     3601 aaaaaaaaag cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga
     3661 aaaaaagacg tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta
     3721 tgtggatgaa gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt
     3781 taacaaagaa tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc
     3841 agtagggcta ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt
     3901 atttgccata cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga
     3961 tatcctattt gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca
     4021 agaatcattt ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc
     4081 tattcatagt tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac
     4141 taaagcattt caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta
     4201 aacgacctgg gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc
     4261 aagttttaga agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac
     4321 ctgtctatgc gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg
     4381 aagatcgttt gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg
     4441 caggaaatga aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc
     4501 ggatatggaa aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa
     4561 atttgctgag ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat
     4621 gacaggaatc gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt
     4681 tttaggtgaa gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg
     4741 ttaccttgtg ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat
     4801 ggctcgtttc cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg
     4861 tcatacgagc aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg
     4921 gattgctcaa gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt
     4981 gcttgtggga ctgctgatga actttattgg tggttggata actgatttta ctactggatt
     5041 tgtatgcaga cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag
     5101 aaaggagagt tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga
     5161 ggtcctttga ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga
     5221 ggtgagaggc caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat
     5281 gggttcaaaa catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg
     5341 actcttagat gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgtttccaca
     5401 ggaaaaagtg gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg
     5461 ttagaacaaa tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca
     5521 tctccagata aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag
     5581 cagcaaaact ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat
     5641 caatcagcaa gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc
     5701 gtttcccttt tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt
     5761 atggcacctt tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg
     5821 ccatttttat ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg
     5881 atcggtgtag agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt
     5941 gctatcaata ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca
     6001 aaagctgaga cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc
     6061 atcccacgag ttatcattgc ttgggtagca agttttggat tatatagctg a
//
LOCUS       DAMEET010000112         6039 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.115, whole genome shotgun sequence.
ACCESSION   DAMEET010000112 DAMEET010000000
VERSION     DAMEET010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.115"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(65..571)
                     /locus_tag="PB780_002346"
     CDS             complement(65..571)
                     /locus_tag="PB780_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDE0734370.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            731..1171
                     /locus_tag="PB780_002347"
     CDS             731..1171
                     /locus_tag="PB780_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HDE0734371.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(1197..2354)
                     /locus_tag="PB780_002348"
     CDS             complement(1197..2354)
                     /locus_tag="PB780_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDE0734372.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(2357..2713)
                     /locus_tag="PB780_002349"
     CDS             complement(2357..2713)
                     /locus_tag="PB780_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734373.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(3011..3985)
                     /gene="sgrA"
                     /locus_tag="PB780_002350"
     CDS             complement(3011..3985)
                     /gene="sgrA"
                     /locus_tag="PB780_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HDE0734374.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(4181..5020)
                     /locus_tag="PB780_002351"
     CDS             complement(4181..5020)
                     /locus_tag="PB780_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDE0734375.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            5205..>6039
                     /locus_tag="PB780_002352"
     CDS             5205..>6039
                     /locus_tag="PB780_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDE0734376.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VM"
BASE COUNT         1936 a         1199 c          893 g         2011 t
ORIGIN      
        1 ttacgtaata aagcagaatg gataaaccag tatccattct gcttttttta aacgaagaaa
       61 caacttattg ctccaaaaat gtcagaaatc gtttatccca aattttctcg taaaaagcga
      121 ttgtttcctt tgctttcata aaaggattat cataagttgt cgttgctcct ctacacatca
      181 taagctggta tcctaatcca tgagcaaact tcacagtcgt atctatacaa tactctgctt
      241 gtgccccaca taattcgatc gaatgacagt ccaattcagt taataaaaca ctcaaattag
      301 tcttataaaa tgaattcgca tgggtttttc tcacaaaata actgtgctca ggaatagata
      361 attccgacaa tagctcccat ggttttgttt gtggcactaa ttgagcatct tcgtgttgaa
      421 cgaaaataac tggtttccca atcgtttcgt atgttttgat tcttttattc actaatgctg
      481 tcactgtttc taattcgtgt atcggcgaag aatcaaaaca tacccctttt tgtaaatcga
      541 tcactactat tgcgtcggct gtgtttgaca tacttgtgcc ttctttcttt tttcttgatt
      601 taatagtatt tcttatctag ctagttccat tatacgtgac aacattttct tattttacac
      661 ctctaaatgg cacttttcga ctatttcttc atgttattat ttcataaata cttagctatt
      721 ggaggaatgt atgatgatta aattgtatac aggttctggc tgctcgtctt caagacgcgc
      781 aaaacgttgg cttatcgtcc atgatatacc gtttatcgaa cagaacttaa ttcgtcaacc
      841 actaaccaaa aatgaattta tccacttact aaaattgaca aacaacggcg tgaccgaaat
      901 gctctctact cattctatta gttataaaaa tcttgttcac agaatagatg aactcagttt
      961 gagtgaacta tatattgaga ttcgtacatc cccttcttta ttgaaaaaac cgattatcta
     1021 cgaccaatct gcaaaacttt tgactggatt tagcgaagat aaaatcagga tgttcctttc
     1081 taaaactaaa agaaagcaag agctcaacga actcttgctt tctggtcatc ttgtcgattt
     1141 taaagaaaat aatctttttg caatgtctta attttggcat aaacaatctg cttttcttaa
     1201 ttaacgaaat ccaatttttc caatatggat ctatttttct cattcctgat ttttatgatt
     1261 tcagctttca gacgcgccca atcatttttt gtaggttgtc cattccagta aaatatctgt
     1321 gtattcgttt gatcatatgc ctttttgtcg ataggataat cgctaatgac aatatcaggt
     1381 aactgatcag cggatgcact atattttatt gggcgttcag tcatagataa tatccttttc
     1441 tttgcccata attcctgtgt tttaccatac tttgattgaa tatagaatat gacaggttgt
     1501 tcgtgtttta taaaaatatc cctgactaac atacagtatt gaaaaatcaa ccgtctgttc
     1561 ttttcataat aagtattaag ctctggaatt ctgcgagcta ccccactaaa aaactgttgc
     1621 atgattggat acatcactgg aaatatatgc tgaaaatctc tgtcttctgc tgattttcca
     1681 aaagcatcca ccttcttttt tgtcccaaaa acccttgaat acaaatggat cattgccaaa
     1741 ttgacatata gataattttt ttctgaagtt gtaaaacaaa tcttgaagtt tttttcaaaa
     1801 ttttctacaa tagacgattc aatccagccc caactttctt gtactttatc tggagtatcc
     1861 aacaaatctt cttccaaata ggtgttcatt acaccaaaca taaaataaag gtaagcttgc
     1921 tctgttggag tgatgtgatt catcgaccag aagtttgctt caaaaaaagg ctctagtatg
     1981 taatgcgtaa actcttcttt tgttatcaat ggatgcacaa atttgttcat ctcttctggg
     2041 acatacgtaa tcgagtggcc ttgtttcatt cgttgcaacg aaattcttag taggctgatc
     2101 catttctttc tatctatact acgcgtatat ggcgcatgtt tcgcaaattt ttctaatagt
     2161 tgacgtttat tttttatact tgataatata tcagaatgat tttcgtcgaa tgactgccaa
     2221 tacatcaagt gaaaaaagta tcggatacgg tattcttctc cttctatcgg attacctttt
     2281 ttcaagctgc aagataaatg atagtttttc aaaatagtat taagttctct taattttctt
     2341 tgaatcgtac gcaaacttat ataatttttt gactaagatt ttctaaggat tgataagtac
     2401 ctttgtaaag tgaatccagt aaaatataac caatcgttcc ctctaaaagt atagaataaa
     2461 ctttactcag taaaaactga gaagaaaact cgacttttat catcttgttt tctgaaagaa
     2521 aagataattt atattctgga tacctttcgc aaaacgattc tgcttgttga atcaccgaat
     2581 tcatcttagc cagggtgtag tttagatcta aagcgatatc cttcaccaca tataatttct
     2641 cgtctctttc ataacaatag ttcaaaaatt gatatacttg cctttcattg atatcaataa
     2701 attcttcaaa caaagtacat actccttttt atctattatt tagacttatt ccgacagcta
     2761 catgaataac gtacaaatag attcgttact gatttttcag agatactacc atgcgcagtg
     2821 tgcattgtca attattcttg ctcttatttt ccatttgtgt tcctgttatt tcctgtttct
     2881 caaatatgtt agtaaacaaa aatagatctg gcaaagctac aacttcgcca ggcaatacag
     2941 cactctcgaa tgaaatattc actcaaaaat ctatcgtttt attcaatttt agatctgttg
     3001 cttttattct ttatttcact cgatggaaga gagaacgaaa atagcttgct cctacaatca
     3061 aaatgagtaa cacacccatc catgtaatta gcttgttcaa ggttctacta ccagtttctg
     3121 gtaaatcgct ttttctatta tctgtttctt tcgctttttc ttgatgatca attccacgag
     3181 aaacaaaatc aggctgtttt agcacacttt ttaggttgct ccctgcattt ctcttttgtt
     3241 ccttagttgg tatgataggt accaaatcaa gttcattatt tacttttcct gtgctgcttt
     3301 cgctgctcgt gcttcctgtg ctactctcac tactcatgct tcctgtgcta ctttcactac
     3361 tcgtactctc cgtactgctt tcgctgctcg tgcttcctgt gctactttca ctactcgtac
     3421 tctccgtact gctttcgctg ctcgtgtttt ctgtgctact ttcactgctc gtactctctg
     3481 tactgctttc accgctcgtg ttttctgtac tgctttcact acttgtgcta tccgtactgc
     3541 ttggcggtcc aacaactgtc caactgcttg gtttcaaatt ctccgtgatc gtcatctgtc
     3601 catcataaaa aacaatcaaa tcaactttat ctttactctt gatctcatca atcgatagtt
     3661 ctgcagaaat cattattgta tactcaatac cagcggtcca ttgtacgtct tttaaataaa
     3721 cttccccaat aactttttct gaacttccat taccatccca accatatgtt agaacagcat
     3781 gtacatcact agcattcacg cttttgtttg gtttgactgg aatagtaatg acttgttgtg
     3841 tcatgtcata aacaatccga tcagttttca cagtaaatga aagattctca tttgcccgtt
     3901 catttgcaga agtactgatt ccaatcattg ctgcaattat actcagccca agaataacaa
     3961 ataatacggt cgctgttttc ttcatttcta tcactctcca tataattacc ttctatttga
     4021 taaaggaatt atataaaatg aaaatgatga gaacacgaca gtcagttgtg aaaatctaac
     4081 aaaagatgtc gtgtattcgc ttgaatttgt ttactttttc gtttaatcaa tgacagactt
     4141 aaaaaagcaa gaccgatatc agccttgctt tcttcatctt ttattgataa acagcagtgt
     4201 atttcttcat ccgctttgtt ttatatgcat agagtaacaa accaacaatc aagactaaca
     4261 agaataatgt agttgatata gatgcttcag ccccaaagtt tcccccagta attattgtat
     4321 tttgcgattg gatcagagtg gagaaaaaac cttgttcttg cgttccgctg actggtatac
     4381 ccaaaatagc tccttgtgtc caattccaag taatatgagc accaatagcc gtaaataaac
     4441 ttcctgtact gtagataagt gcgctgaaaa agcaacctgc taaaaagata ttgaaaactg
     4501 ccagaaatgt catgttagga ttcaatccgt gcaaaagagc aaataatgca gcagatccaa
     4561 tcattgctat taacggacca aatctaagca tctgatgctg aaaaaagcca cggaataaaa
     4621 gctcttctga tgcccccact gcaataaata aaataaaact cgttatcagc ggcacaatgt
     4681 ttgcgtttcc tgaatctaac cgatacgccc ccgtcaaata taatggacta gtaataagca
     4741 gaaacactcc aattccaaca acgagacctc ccgctaaata cccccagtct tttcctcgaa
     4801 cagaaacacc ataatcttct attctttttc cttcgacctt actagcaaga aataaagcta
     4861 gtaaaaaaac agctaaatgt ccgaccgtac cggcaattgc tccaatccat tcactatttc
     4921 caataaacat atccgcaagt gcattattga caagaattcc taaaacaaaa ccaactaaga
     4981 ctattaaaac agatccaatc aaccgaataa tcctgatcat ctttcatccc tcctttatat
     5041 taagtttaac aacccttttc tttcttgtct cgtgaaaact atgtaaatgt tctccatcat
     5101 agagagaata gatttttacg aactaagaat atctacagca accttacgaa aatgaaagac
     5161 tgtctcccta attcttctgt tagactgttt ttaataagga aaggatggga aattttttga
     5221 aaaaacaact actaattatg tgcctttcag catgtgcaat tttactattc tcaggttgta
     5281 gcaagtccaa tgaaacgatt gcaacgttta aaggtggaac acttacagtt caggattttt
     5341 atgataaccc aaaagttaaa tcaaataatc aaacactatt aaaaaaaatg attgtcttca
     5401 aagcatttga tgatgtatac ggaaaagaga tttcttcaaa ggaagtaaca aaagaatata
     5461 atgaacaaat caaaaaactc ggtccaaact acaaggaaca attaaaagca gttggccaaa
     5521 cagaagagac atataagaaa ctctttaaac agatgctggc tttccaatac ggtttgaaag
     5581 ccaatgtaaa attaacagat aaagatctag acactgcttg gaaagaattc tatccagaag
     5641 ttagtacaca gatcattcta ttttcgacta aagaagaagc agataaagca aaaaaagaag
     5701 caaatgaagg agaaaatttc tccaagcttg ttcaagctta tggaaaaaat aaaacattaa
     5761 aggaaactga tggaaaaatg aattttgatt caaccaatcc agagattcct actgaagtaa
     5821 aaaaagcagc atttaaacta aaaaacgggg aagtttctga tatcattcct gtaacagatc
     5881 caacgactta tcagcaaagc tattatctag taaaaatggt aaaaaaacag gataaaggtt
     5941 ctaataaaga caaatacaaa tctgagctag aaaaaattgc taccgaagca aaaactcttg
     6001 atacggaatt catggataaa acaatccgta aagtgatga
//
LOCUS       DAMEET010000113         5952 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.116, whole genome shotgun sequence.
ACCESSION   DAMEET010000113 DAMEET010000000
VERSION     DAMEET010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.116"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(31..906)
                     /locus_tag="PB780_002353"
     CDS             complement(31..906)
                     /locus_tag="PB780_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDE0734377.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            1054..1386
                     /locus_tag="PB780_002354"
     CDS             1054..1386
                     /locus_tag="PB780_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDE0734378.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            1498..2706
                     /locus_tag="PB780_002355"
     CDS             1498..2706
                     /locus_tag="PB780_002355"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDE0734379.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPSLKEEGEALLRFAELPSVPLKEESQVWTLLISQLGLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            2860..3315
                     /locus_tag="PB780_002356"
     CDS             2860..3315
                     /locus_tag="PB780_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734380.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            3677..3952
                     /locus_tag="PB780_002357"
     CDS             3677..3952
                     /locus_tag="PB780_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734381.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            3965..>5952
                     /locus_tag="PB780_002358"
     CDS             3965..>5952
                     /locus_tag="PB780_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDE0734382.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRHADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINL"
BASE COUNT         1924 a         1005 c         1257 g         1766 t
ORIGIN      
        1 tttcctcata gcctatcttt gtcttttttc ttatagtttt ttcagtaatc ttgtctgtgg
       61 cttcaaataa gtaaagcctt gcccttccac ttcatgcaca ttgatgattg aaaggaaagc
      121 tttttcgtca atctcgtgga cgatcctctt agcttcagtt aattcactag agctaacgac
      181 catatagatg attttttttg aattaccaga gaatgcgcct tctccagaaa gaaaagtcac
      241 acctctttgc agaccggtca tcagtagtgg tgcaatctct tcgcttttgt tggagacgac
      301 aagtagacct tttgcagaat atccgccatc taagatcgaa tcaatcacac gactgaaaac
      361 atacgaaaca atcaacgtat acatcatacg tttcaagtcg atataggtta atgaagcaag
      421 taagacgatg atatcaaatg ccaataggga acgccccata gtgatgccgt aattttgttc
      481 taaaatccga gcaataatat cgctgccacc tgttgttcca cccatgcggt agacgatccc
      541 gcttcctaca cctgcaacta aacccgcaag tagagaaacg atcagtagat catgctgtaa
      601 attgatttct agtggaaccc tttgccagat ccataaaaag aatgacaaag aaagtgtacc
      661 tagaactgta taggccaagg aacgtttacc gaggatcttt cctcctagta aaatcaatgg
      721 gatgttgatt aataaagtcg tataagctgg atcaattccg aataaagctc ttaagatcaa
      781 ggtgatccct gagacgcccc cttctgctaa atgattagcg atgttcaagt aaacaagacc
      841 aaatgcataa atacttgttc ctataatgat aaaaagaact tcttttactg tttttcgttt
      901 gaccatatga attccagctt tcaattatgt attttcacag aatttccatt tagaaattcc
      961 tttctaaatg tagcatggaa attcgatttt ctcaactgct cttatctagg atttgcgtta
     1021 aagttttggt aagatagttt tggagggaaa aagatggata aaaagcgttc tttatacagt
     1081 atgcagcagg aagtggatga atatatccag caattcaagg tggggtattt ctctccatta
     1141 gctcagatgg ctcgattaac tgaagaagta ggtgaattgg caagagaagt caaccacact
     1201 tatggagaaa aaagcaaaaa ggcttctgaa ccagttaact cggtagcaga agaacttggt
     1261 gatgttttat tcgttacgat gatcatggct aattcattga atatcgactt aactgaagtt
     1321 tttgaaaaaa acatggaaaa attcaatcaa agagaccata atcgctttga gcgtaaggac
     1381 gagtaaacaa gatgaagtgg tcctggcctt gaagactctg aaacggactt gtcaaacagt
     1441 caccatgcaa ctgagtcttt atctcagtac aagaaacacg caggaaaggt tggaaaaatg
     1501 aaattaaaac aattacctct ggaataccaa aaggcaattc cagttctgaa aaaattagag
     1561 aatgcaggat atgaagctta ttttgtaggt ggaagtgtac gtgatatctt gctgaaccag
     1621 ccaatccatg atgtggatat cgcaacaagc gcatttccag aagaaatcaa acagcttttt
     1681 ccaaaaacga tagatattgg gatcgaacat ggaactgtgt tggtattaga aaatgatgaa
     1741 caatatgaga tcaccacatt tcggacggaa tcaacttatc aggactaccg cagaccagat
     1801 catgtcgaat tcgttcgttc gttggcagaa gatttgaaaa gaagagattt tacgatcaat
     1861 gcgtttgctt tgaaagaaga tggagaaatc atcgatttgt tcgatggtct gcaagattta
     1921 gaagaacaga tcttgcgagc tgtaggcaat cctcatgagc ggttccatga agatgcctta
     1981 cgaatgatgc gcgggttgcg ttttgtcagt caactagggt tcaaattaga ggaagaaacc
     2041 ttctacgcca tcaaagaaaa ccacgagcta ttaggtaaaa tatctgtaga acgtataaat
     2101 gtggaattca tcaagatgct cttgggaaat gaacgggccg cgggaatcca tgcatttatt
     2161 gaaacagagt gctacatttg ctgtcccagt ttgaaagaag aaggcgaagc acttttacgt
     2221 tttgctgaat taccatccgt cccattgaaa gaagagagcc aagtatggac attattgatc
     2281 agccaactgg gtctaaaaga aaatgagatt cgttcctttt tgaaatcgtg gaagtgttct
     2341 aaccaaatga ttaaagacgt tcaagcactt gtccaaggac tacgacaacg agaaactgga
     2401 gctttggaac cattcgaatt gtacttctta ggcaaaaaac atgcactgca agtagaagaa
     2461 ttgatgatct attttgacga aacacctcag atggatgccg taggagaatc ctatgatcgt
     2521 ttacctatca agtcattaag cgaattagca gtagatggta aaatcttgct agaagaaact
     2581 ggaaaaaaac ctggtaagtg ggttagtgaa gctttgcaat tagctgaaaa agccgtcgta
     2641 gaacaaagaa taaaaaatga taaaaaacag gttttggatt ttttatcgaa agaaaaattg
     2701 atttaaaaaa cactagactg tatctgctca gctttgttgg ctacttaaag tgggcagata
     2761 cgtcattaga agaaaaaaca taagatgtga ccacttacat aaatttgtgc taaactgagg
     2821 tagtacaaga ataaattaca tcataaagga gtctttttaa tgagtaaaga aatgacagcg
     2881 ctaaaatttt attttagaaa tggagaaacc tggacgatca accgccgtca tatcggcgat
     2941 ctttggatca aacaaatcac aacaagtttt ggacgaatca atggcagtga atttgttgaa
     3001 atccatccgt gtgctggatt taaaatcgaa atcttccatg aaggggacgc agttgcgaca
     3061 catgatatca acctaggcgg actggaaatg ggcatgttta atcgcgcatt gaaatacgaa
     3121 gatatcgagc gaatggaaat cctttaccga aatggcacac ctgatcttgt gtacttccca
     3181 tatctagata aaggaacaga aggattagac aatcaatatc aatcaacaaa aatcagcgaa
     3241 aaaacaggta acttgtatat cgtgatcaat ccagatcaac gtgtagaaga tgtatacggg
     3301 gaatttttcg aataaatcga tttttacgca aaaatgaagc tgtgaaagag atgtgtctta
     3361 ttactactga taggctgtct cttccacacc gtttattcag aataagggac tgtgacaaga
     3421 tttttgtcgc agccccattt ttttgttgtg ataaaagaac aagaaaggca ggaatggaaa
     3481 agtaaacatt cttttgtcag aaacttttat ctttcttaag ttttacttga tatctgatac
     3541 tatttggaat agaatgaggc gttgaaaaat ttatggaaat ttctaactag caataaacga
     3601 tgaaacaatg aaatattttt catccaaaaa gaactttgct ataatgatgg aagtttgtgt
     3661 ataggagatc tggaaaatga gagagagttt tgaacaacaa aaaaaactgt tgcatgatcg
     3721 ctatggcgcc ctttcgatgg atgaccgccg tcaaatactt tgtaagttgc gtaaaagaaa
     3781 tatattgatg tatcgtcagt tggaacgtct gaaacatgat ctgcttcgtt tggagtcaaa
     3841 acgtgtacag tgtgaattag agggtaacca aacacaggtt gaagtagtcg aaaccaaaat
     3901 tttaaagaaa aaagaacaat ttttaaaaat gcttacacaa aataaaaaat aaaggggatg
     3961 gacgatggaa tttgctgagt tagttattgt atttgcagta acgatcacct tatcgaacat
     4021 agctagccgc attttgccaa tgattccagc tccactgatc caaattttcc ttggagtggt
     4081 attaggtctg acagagtggg gacaatcaat cgattttgaa cctgaattgt tcttagttat
     4141 gattattgca ccattgttat ttagagaagg agaaaaagca gatatttctt cgattctcaa
     4201 aaatttcggg acaattttgt tcttggcatt tggaggagtc attttgaccc ttgtcggggt
     4261 aggtgcgacg ttatcattcc tgttgccaag tgtcccactt gctgcctgct ttgcttttgg
     4321 agcagcgtta ggtccgacag atgcggtagc agtcagttct ctttctggac gagtgaatat
     4381 ccctaaaaag gcgatgcata ttttggaagg agaaggtctt ttgaatgatg catctggtgt
     4441 tactgccttc cagtttgctc ttggtgcgct gatcaccgga tctttttcag cagtaaacgc
     4501 ggggatgagt ctagtagttt ctagtattgg gggagctttg atcggctttt tgcttgtctg
     4561 gttcaaacaa aaaataattc atttgatcga aaaagcatca gcacaagatg tcacagggta
     4621 tctgctgatc gaacttttgc ttccttttct agcctatgta ttggcagagg catttgatgt
     4681 atctggaatc attgctgcag tggcagcagg tattctccaa gcttctggtt ttaggaaaat
     4741 ctctgtgttt gatgcagaat tgtcgagctt gtcccacagt acatggacaa ctattgcttt
     4801 tacactgaat gcattagtat ttatattttt aggaatcgaa ttgacgcaag tgttctcgcc
     4861 agtatgggga gacggtttat atccaaacgg tcttttatta gcgattatcg tattaatctc
     4921 tgtcatgctt tttgtgatca gattcatttc tatcagtctt ttttatgtat tcaaagatgg
     4981 cagtaaaaag ttcaaaaaac agttgaacga gatcttgatc ttaacatttg gcggtgtaaa
     5041 gggaacagtc agtttggcta cgatttttat tttgccattt tcaatcaata atatgatgtt
     5101 ttaccagcgg tctttgcttt tgtttctgac ggcgggtgtc atcttagtga ctttggtcat
     5161 agggatcatt gtcttgccta tgctgacgga aacagaggaa gcgaaaagca cggatttgaa
     5221 tgctttgatg attttagaag aggtcgttga agttttgcga aaagaaataa aggaaattga
     5281 tcgtaataca aaagaatttt tagccactga agcagtgata gaaaattatc aagaacggat
     5341 acgtgatctg tacttggaag atttaaccga tgatgaaaag caagaagtac aagaaataca
     5401 agcactgatt ttgtcgatcg aacgggatgg attggatgaa agttatcgtt cgggcaagct
     5461 ttcttctaat ggttatcgtt tttactcccg ttttctttct cgattcgaac attctatcac
     5521 aagccagatt ttgtctttta tcggcttttg gttcattgtt gtccgtaggt taatgcgaat
     5581 cgttctccat ccgaaaatgt tttggcaaag aagacatgct gatcgtcaga catttattag
     5641 tgatgaagat atccaagaaa tcagagagac atatcaaaaa aatactcagt tgattattga
     5701 aagtttagat aacttgactg atatttatga tgaaacgatg gttcaatttt ttatccagca
     5761 acgcaaactt gaaggaataa aaatgaactc ggggaatttg atttcttcgt ggatgatcca
     5821 gcaagatagt ctgtttacaa aaaaaatgct aagaggttat tatcttgaaa gaaaaatcat
     5881 tgatgaatac gaagttgctg agaaaatcac gacgttttct gcgaacagct atcgcaggaa
     5941 tatcaatttg tt
//
LOCUS       DAMEET010000114         5897 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.117, whole genome shotgun sequence.
ACCESSION   DAMEET010000114 DAMEET010000000
VERSION     DAMEET010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.117"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(174..476)
                     /locus_tag="PB780_002359"
     CDS             complement(174..476)
                     /locus_tag="PB780_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734383.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(696..1062)
                     /gene="ssrA"
                     /locus_tag="PB780_002360"
     tmRNA           complement(696..1062)
                     /gene="ssrA"
                     /locus_tag="PB780_002360"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1233..2006)
                     /locus_tag="PB780_002361"
     CDS             complement(1233..2006)
                     /locus_tag="PB780_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDE0734384.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2150..2500
                     /locus_tag="PB780_002362"
     CDS             2150..2500
                     /locus_tag="PB780_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDE0734385.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2481..3197
                     /locus_tag="PB780_002363"
     CDS             2481..3197
                     /locus_tag="PB780_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDE0734386.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3197..4645
                     /locus_tag="PB780_002364"
     CDS             3197..4645
                     /locus_tag="PB780_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDE0734387.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4715..5224)
                     /locus_tag="PB780_002365"
     CDS             complement(4715..5224)
                     /locus_tag="PB780_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734388.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5214..>5897)
                     /locus_tag="PB780_002366"
     CDS             complement(5214..>5897)
                     /locus_tag="PB780_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734389.1"
                     /translation="KKQTILYCLFSSFSAFLLIACLTLYHLQSALANQVQDRLSFIGA
                     SASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNKQELINWHLTGLSKT
                     KLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVLKTTELPDIRDILTS
                     VSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLILSLFPFTFFIHHLQ
                     SRRPGRNET"
BASE COUNT         1998 a         1030 c         1141 g         1728 t
ORIGIN      
        1 acttataatt ggagtggtag ttaattagtg acttattttt gattttatag cactgatact
       61 ataaaatata gatatcatca tattacacaa tcttaatact aacttaaaaa atatcccttt
      121 tataagtatg gtgataaaat ccgttccggg ctaccttttt taggtagcct actttaatct
      181 ttataccttt ctggatcaac gaaagtatac ttcaaatagt cataacgccg atgatcgctc
      241 cgtgcgtctg gcacatcagt cacgatatca aacaaaaaat acacatcttt cttcattctg
      301 gtttttgttg ccggaatttt aaagtagttc ttattagagt aatagagatt gattaataag
      361 ctatcttcga ttgctaaaaa gaaaacttct gaatcccaca ccttataaaa atctttgaca
      421 aatctattcg aaggatcaaa tttaaaccat aattgtgttt ttccttccat caacataatg
      481 ttcacctcaa aaagagtata cgaactaatg ttctttttgt aaagtaggaa tatcattgat
      541 aatatggatg gaacatatat attaaaaagt gttctttttg aatgctattt tgaatgccat
      601 tttcattcat ttaacgaaaa tctgcgaaaa taaaaaatgc tcaaaccctt ataaaataag
      661 gatttaagca tctagcgaaa tctgctgaaa tctcacaatg gagacggcgg gagtcgaacc
      721 cgcgtccaaa cacatcgcca cttaaaaatc tacgttcata gtcaactcat ttaaggattc
      781 actttacagc ttgccgagtg acaggcattc tgtatcgcta gtctgatgtt ctctttttca
      841 acttacagac ggaaagttga aacgtatccc actaaagtta ggacccttac tcgagcacat
      901 gggcgatgcc gagaggatct acgctaactg tttttaggca gctaaagcgt aagagttgtt
      961 ttcgttttta gcagttatat ttaactgtaa cgtttttacg tagacgtaac ctacgaaacg
     1021 caattcaagc tcgactgtgc ctgtcgaatc cgtaacgtcc ccgttagcta acaagggtta
     1081 cgtgtatttt tcatacaaaa ttagtatagc atatattggg ataaattcaa taaaaaagcg
     1141 aatcatatcc cattatttat ataataactg cccctttctt aagaaaaaat aagcgatttt
     1201 cttaagaaaa gaggtagctt caaaaaaaga tcttaaatca gatcaaaata cttcaatgcg
     1261 tggacaattc cgccttctgt atttttcttt gtgataaagg tagaaagttc ttttaatccg
     1321 tcataggcgt tgcccatagc gattttgttg tcgcaagctt ccaacaaggc aaaatcattc
     1381 ggaccatcgc caaatccaaa agtaggaact ttttccagct gaagttcttt ttgcagacgt
     1441 ttgacacctg ccccttttga gattcctttc ttcactgtat cgatcgagta aggaccattc
     1501 cgataaaaag ttagctcagg aaaatgttcg tgatagtatt cgtcacctga ttgagataag
     1561 atcagcaaca tatttactgt gttttcttta aagcctaaag gatcaatttc cggaacatct
     1621 gaatgaatga agtcgtaagc attgatcatt gtttggctat gaccggtaca ccagattttc
     1681 tgatcgttat agtaagaaag ttcatgaccg ttcaactgta catgatcata cattttttga
     1741 cattcttctg aagaaaatgt ttgagaatag atttcttcgc cgtctactcg aatgaaagaa
     1801 ccattcatgg tgaccgcaga agtaatgccc gcatcttcca tgatagagtg gatttctgct
     1861 tctgtacgtc ctgtagcgat gacggggaga acattgtttt cttttaatgc gttcatagct
     1921 tttgccactt cgggtgtgat ttttgatttc gcatctagta atgttccatc aagatcaaag
     1981 aaagtgattg cacgataatt tgacacaaat aaaactcctt ttttattgga ataagtttat
     2041 cttaacaaag aatgaacaaa agacaaacct ttcgtgaaga attcctgaac atattggttc
     2101 agtcaacaat ttagagtata ctataagaag agaatttagg agggaaagaa tgattttttt
     2161 cagtattggt atcttattct taataattgg cagtcttttt cggattctgc cctcaaaagg
     2221 aaatctgcct ttttatgggt atcattcgcc tcttgccgca aaaacagacg ctcattggcg
     2281 gctggctcaa aagacaagcg gaaactggtt ttttcttatg ggcttactga tggcattgat
     2341 cggctattat ttaaagacat ctgggcatac gaattatttt ctgattgaaa tgcttctgct
     2401 agtatttcca attatgccta tttttattat gacagaaaag aaattacaga aatatgacct
     2461 ggaaactgga gggaacgata atgaatattt taatgattga agataatcaa tctgtttctg
     2521 aaatgatgca gatgtttttc ttaaatgaag gttgggaagc aactttcaag tatgatggaa
     2581 aagaagggct cgatgccttt ttagaaaacc cacaacattg ggatatgatt acactagacc
     2641 tgaatttgcc cacactggac ggtatgctag taggaagaga gatccgcaaa gtttcaagta
     2701 ctgtgccgat tatcatgctg acagctagag attctgaaag cgatcaggtg atcggcttag
     2761 agatgggggc agatgattat gtcacaaaac ctttcagtcc tctgacgctt attgctcgaa
     2821 tgaaagcttt gcatcgccgt agtgagttgg cagaagcaag agaagaagca gtagtttcag
     2881 atgatcattt tgatatcgag actagtcatt tcaagatgaa tacgaaaaca cgagaagcgt
     2941 atctggacaa ccggccaatt gaaggactga cgcctaaaga gttcgatcta ttgtttactt
     3001 tagcaaaaaa accacgtcaa gtattctctc gggagcagtt gcttgaatta gtctgggatt
     3061 atcagtactt tggtgatgaa cggacagtgg acgcccatat caagaaatta cgacaaaaaa
     3121 tcgaaaaggt cggaccacaa gtcatccaaa ctgtttgggg agtcggctac aaattcgatg
     3181 attctggtgt tgcctaatgc gttatttgta tcagcaactc ttagctttct gcggaatggt
     3241 cgcgttgatc ttattgatcg ttggagtttc ttttacacaa ctgacaaaac aaacgattga
     3301 agaaaacaat taccagcaac tgtttggata tgctgaatca gttgaaaaga caacgcaaac
     3361 ctatgccgat acgctcccgc agtttagtca agatcaggcg tttcaaaatg cattgctgct
     3421 gacagagcaa gtactgacag aacaaaatgt gaactttata tttatcgata aaaacgaacg
     3481 tgtcatttat ccgactaata cagctttact gaattttacc ttaacagctg atcaatggaa
     3541 aaatctgagg aatggacgtc aagaaaaatt cacatcgaat aaaaatattt taggacaagc
     3601 acaggcgacc tcttacgcac ttgtgccatt taatttgaat catgaattct atggagcact
     3661 tgtagttagt caacctgcgc gaaatatcga taacagcgtt cgtccaatta cactgaacct
     3721 gttcaaagga tttatttttt ccagtatcgt tgcagttatt gccagctatg cttttgcagc
     3781 attccaagtt aagcgtatca atcggttaag gaatgcgacg aaagaagtga cgaatggtaa
     3841 ttttgatgta cagctccccg tacatgacaa agatgaattt gatgagttag cagatgattt
     3901 caataaaatg acgaattcat tgaaagaatc tcaagcagaa attgaagaac aagaaaatcg
     3961 tcgaagacaa tttatggcag atgcttctca cgagatgaga acacctctta cgacaatcaa
     4021 cggacttctg gaagggttag aatacaatgc aatccctgaa aatcagcgag aaaacgcgat
     4081 taaattgatg aaaaatgaaa cagagcgtct gatccgtttg gtgaatgaaa acttggatta
     4141 tgaaaaaatc cgtacgaatc aaatcagtat ggtcattaaa aaattcaatg gaacagaaac
     4201 attgaaaaat attgttgcac agttagaagc taaagcagaa gcagcaggag atacattgac
     4261 attgaaagcc gttgatgata ttgatgttta cgctgattat gaccgatttg tccaaatcat
     4321 ggtcaatatt attcaaaacg cgatacagtt tactgaaaac ggccaaatca cgattacttt
     4381 agaaaaaggc tatcttgaaa cgatcatcac gattgaagat acgggaattg gaatgtctga
     4441 acaacaaatg aaaagtatct gggatcgtta ttataaagta gatccttcaa gaaaaaatac
     4501 aaaatatggt gaatcaggat taggtttacc aatcgtgcag caacttgtac gcttgcataa
     4561 aggaaaatta gaagtagaaa gcgaactagg aaaaggaaca aaattcgctg ttcgtttgcc
     4621 tgacgtagaa atcaaaaaag agtaaaaaac aagcggtata aaagatcatg aatcgatctt
     4681 ctataccgct tgtttttact ataaaatgat tgatttagcc cgcgggctct aaacgatcaa
     4741 tataaggact ttgagcaata gtagcatcat gagtaaagag taagatacct tgattttctt
     4801 catgagctaa ataagaaagc aagtggagta gctcttggat ctccaataca ctaagttcat
     4861 caaatgtatc ttcaataaca atattgtttc gcttggaaag caaggcatga agtaattgca
     4921 gctttacttt ttcaaaagac gtcaactcag ctgccgcaca attaagcaag gaaggagaga
     4981 tatcaagttt ttcttgatag gccaaacgat cttgtttgat ttctttttcc ggtacatcca
     5041 acaggacatg gtctttaaga ttcagataag gaaacatcgg ccaatttttt tggatgatcg
     5101 caaaattatc cagttttgca acaagaacct cttttttcaa gtgctccaat attttatgct
     5161 gtttttcgag aggacacaaa ataacggtca agtgttcagc atgaatcaaa tattcaggtt
     5221 tcattacgtc caggcctcct actttgcaaa tgatggataa aaaaagtgaa agggaataga
     5281 ctaagaatga gacagcatcc actgagccca ataagagttt gggcaaatcc cttaagtata
     5341 tctgtgaaaa aaagatcatt ttggaaatcg atggtaaaaa aagtctgttg atccatcgga
     5401 attgtaagtc cgcttaccga agtcaagata tcacggatat ctggcagttc agtagtcttt
     5461 aagacaagaa aattgatcct ttgaagaagc gcttcataaa ctgactggaa gcttacgatc
     5521 cacaataaaa ccaagataca acaaagaaag agaggaaaga acagctgcca gaaaataaaa
     5581 aggaataatt tggttttaga aagaccagtc aggtgccaat tgatcaattc ctgcttgttt
     5641 tgcctgactg aatagtaaaa gaaaaagata aaaaggaagg caaatagaaa aaataaactg
     5701 aatatgcctt ttatataaaa tcggctagct aattctaaag ccggaagaag ctctgtagat
     5761 gcagaagcgc caataaatga aagccgatct tggacttgat tggctaaagc cgattgcaaa
     5821 tggtaaagag tcaggcatgc tatcaataaa aaagcagaaa agcttgaaaa taaacaatag
     5881 agtattgtct gcttttt
//
LOCUS       DAMEET010000115         5771 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.118, whole genome shotgun sequence.
ACCESSION   DAMEET010000115 DAMEET010000000
VERSION     DAMEET010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.118"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            14..1168
                     /locus_tag="PB780_002367"
     CDS             14..1168
                     /locus_tag="PB780_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HDE0734390.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSLTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKIARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1162..1584
                     /locus_tag="PB780_002368"
     CDS             1162..1584
                     /locus_tag="PB780_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734391.1"
                     /translation="MVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2717..3685
                     /locus_tag="PB780_002369"
     CDS             2717..3685
                     /locus_tag="PB780_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDE0734392.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
     gene            3695..4183
                     /locus_tag="PB780_002370"
     CDS             3695..4183
                     /locus_tag="PB780_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDE0734393.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            4479..4760
                     /locus_tag="PB780_002371"
     CDS             4479..4760
                     /locus_tag="PB780_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734394.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            4762..5178
                     /locus_tag="PB780_002372"
     CDS             4762..5178
                     /locus_tag="PB780_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734395.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
BASE COUNT         2146 a          982 c          948 g         1695 t
ORIGIN      
        1 ttaacacaaa ggcgtgatta ttttgaaacc caaaaagaaa atttatctag gtctcctctt
       61 ttccttgatg attggaattt ttagtatagg aattacaaat acctatgctt cagaaaattc
      121 taatttcaat atcaactccc aaccaattga agaacaagca attaattttg cgaaaactga
      181 ttttgaacac tccataactt caattctttt aatggaaaat aacaccgaaa ctgatattaa
      241 ctatactctt ggttcgccat ttaaaatgct cgatggagaa aatacggcaa atagttctat
      301 tttctttcca gtattttcag acgctgataa tacgattaaa tatgtttata ccgtcaaaaa
      361 aaatacaaat ggagaatatt caggaagtat ttcacaattt ttagcaaaag aacttcaaaa
      421 tctagtagat agtaatataa cagatacaac cttatttttt caagataata gcatttatta
      481 ttccgataat ggacaaatta aactactttg gaaatcacct ttaagtacag aaaaaaatgg
      541 tgtttcttcc cttacagaaa aaaatgatct tatatccgta aatataaaaa ataaaattaa
      601 aaatttttct aaaaaacaaa attttcctgc ggttaaacgt gtaaaaagtg tctcctccgg
      661 agataacttt attatgatta attggcgtat agcagaaacc caaacaagtt ttccgtggtg
      721 cgctgcttac gtagaagctg ctatcttatg taacaaaaca gatgtacgtg ctacttcagc
      781 tagagccatt atgcattata cctatcctaa tttaaacaac agtcaaattg aatcaaaaag
      841 tcttaatcaa gatcaaataa taaaatatgc aaattactgt ggtgtttatc ctaaaaaaat
      901 agcacgaata ctaaaccttt cagaggtcca aacacaacta agaaaaggaa atgccattta
      961 tatgggaact gctggttatg gaaattatag taacagccgt catgcacttg cattatttgg
     1021 gtgggtcaat caaggtggtt atcaaactta ctatgtgtgg aatccttggt ataactaccc
     1081 aactgtaata agtgcaaatg caggaccagt tacaattcct gtacctggag gtggttttac
     1141 ttgggacaat accattacaa attggtaaat aaactcattg ctttagttat gggtctagtg
     1201 ttagttcttt tcttaactag ttgttcaaaa aaacaagaaa tacaaaagac tgatctgatt
     1261 ccttttctag gaacttggca aactaaagat cattcagaac gtctagtaat cactaattta
     1321 aatacttcaa aaaatataga acaatatacc atcttagtaa aagagaacaa tcataaaatt
     1381 caaatcattg ataaaaattt aaatataaat gaacaagttt tcatttacaa agcaacaaat
     1441 aatttactaa cactgcgctc gaataaaaaa caattagatt acacttttag tttggattct
     1501 ccaaaaaagc tcgtatttat tttgacaacg aatgatggga cagaagggac agctaaacca
     1561 attgaatttt ttaaagctga ttgatgaata gttgtttgat attacaaaac aataatttca
     1621 ccgaaggtga aacaagcgaa actttacaaa aagtggctag catttgctag ccttttttgt
     1681 attgtggagc gtgggttttg agggggattc aagggggagc ttgcgcccct ttgacaagcg
     1741 gttatttgca ccaaatacgt aggtatttgg ctagcaaata accgcttgtc aagcccttta
     1801 gtgtgttttg tagaaaggga ccttatagtc actttcacat tacaaacgat acgtcctatg
     1861 gacgtatcaa aagggtggga cttatgagca aattaaagca aaatttggct agcaatcctt
     1921 ctaaaaaata cacctattga tcagagtcaa aataaatcag ttttcgagtg agcgaatcca
     1981 aatttgcaac tctgaagctt tgcaaatgag tgtgccggct tccgtaaaag tctaggtaac
     2041 attagtcgat atatacatag ggctctaatt tgcgttttaa ggcgttttaa acgttctggt
     2101 atgtaattat accaaagcga ctttaaaacg ccttaaaacg aattttagag gttctgtaga
     2161 acgttttagg gttgttaggg cttgccctga ccgcctgtaa ggcgctttaa aaacaagata
     2221 agggatttta tctagacttg tcttaaaacg ccgtagatga gcgctcagag cttctaaaag
     2281 gactccttac gaactgtttt taagcaaagg agcctttagg cgactgcgca gtttaatgtg
     2341 agagcgtagc gatcacacct ttaaagcgac cgtagggagc agattcccag tttaggaaat
     2401 ttcgcgaacc tgtttggaga aaaaaagtag gaaaatttta ggcgggaaat tttgcccccc
     2461 tcttttataa tctcttttat aacctctttt taaacctctt taccgtcggc tgtatcctta
     2521 ctctcccaag gtgtgcagaa tcttttaggg gtctttttgt ctgtttttag gggtcttttt
     2581 gtctgttttt aggggtcttt ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt
     2641 ctttaattag ggggttgacc cccattagaa agctctttat aattttgtta agtagttaat
     2701 cttgaaggag gattttatgg caaatgaaat tgtgaaatac caccatgaat taaatacgat
     2761 tcctttacgc aaatttacgt cagtagaaat gaatttattc ttctctataa tatctagaat
     2821 gcgtgatgta ggcgataaaa aagtacaatt tacattcgaa caattaaaag atttaagcaa
     2881 ttataaagca actgcaaatg ttcgattcat agatgattta gaaacaacgt atgacaaatt
     2941 aatggattta cgttttggta gaagaagtgc tgacggtttg caacgtgaac gatttgtttt
     3001 atttaatcaa tttaaaatcg atggaaaagc tgatattcct tttgctgaaa tacaagtcca
     3061 tgagaaagcc ttacccttac ttaataatct tgaagaatgg gttcgatatt ctctccaaca
     3121 attcaatgaa ttagaaagca gttattccaa aaccatgttt agattattaa aacaatacag
     3181 aaccaaagga attgccaatt tttcaaaaga agactttcat gaattacttg atatccctaa
     3241 aagttataga gaaagtgata taaataaaaa aattataaag cctattcgcc aagaactaac
     3301 cgcaatcttc aaaggtttga caatccaaaa gagatacgga aaaggacggg gaaagcctgt
     3361 cattggctat caatttacat ttaaacctga gataaaaaaa tcagacgact ttaacaaaca
     3421 tgctccaacc aaagaagaac aaaataaagc tttggaacta gctaatcaac aggttcaatc
     3481 tgaaaaagaa cctacttcat tacaagaaaa aatcaaagaa caaaaacgaa aattagaact
     3541 tttacaacag ttagcagaat tagaaagaaa acaaaatacc ccaagtaaga aaattaacaa
     3601 acaaatagca gataacaatt ccgatattac cccagaattt aaagaaaact tactagataa
     3661 tctccaaaac ttatttaaag aataaggggt ttttatgaca gataaaacta tcaaagaatt
     3721 agcagatatt ctaggtgttt caaaagataa agtgaagtat caactgagga aattacctag
     3781 taattttacc tacaaaaaag gaaatattac ctacctgaaa aaagaagcag taacaacaat
     3841 tacaaaggtt ttatcaggta atgaatcggg taacttacct agtgaattac ccgattcatt
     3901 acctaccgat tacctactca aggagattga aactaagaat aatcatattg aagaaaaaga
     3961 taggcaaatt gaaaaacttc aaatagcttt agaaacgcag caaaaattac tcgaccagca
     4021 acaacaactc accctacaag ccaatcaaca aatcaaaaaa cttcacgaac aactccagct
     4081 aacctatgca gaagaatctt cagaaaaaga acccctttct gttactgaag aaactcaaaa
     4141 aggacaaaac aaggagaaaa aatggtggca attctggcaa taacccaaca atttttattc
     4201 ataaaaattg ttgggttact ttcttttttt gcttttacaa gcatagcgac atgatctcac
     4261 gtcgctattt tttaaagagc ataaggaaac gcggtatcac tttcttacga aaggtgctag
     4321 gtaggctgtg cctaccacgt ttctaagaaa aaaatttgct atcacttttt cgtcattgtg
     4381 gatagcttat gtatagtgtt tcggcaaaaa acagctatac aaatactata tttgtatgat
     4441 atacataaat tatacacaaa tgcgaaagga attcttctat ggacatttta gttcggaata
     4501 ttgatccaaa agttatttca gaaatagata gacgttgcca agaactcaca acaaaaactg
     4561 gcaaaaaatg gagccgtaat gcgtacctaa aagttctaat cgaaaacgat tttgaccagg
     4621 cacttaggca ttacaaaaaa gatcaattcg atttattact cgaaaaattt atcgatatac
     4681 aagcaaaaaa tactgaaaca ttgaacgact atgttcatac aaacaaccaa ctcattgaaa
     4741 cacttatcgg aggaatataa catgagaaaa acttctgttc aaaaacacgt taccctttat
     4801 cccagtcaca ttgacgtttt agattccatc atcgaaaaaa atccagatat caataactac
     4861 tcggaagcca ttcgttttct atgtatgagt tatgaaccta atactaatac gacaactacc
     4921 agcaaccaag caaaactaaa tgcgatggga aaagaaattt ctattatgtc cgaaatcatt
     4981 gcagaatttg ctgatttata tctaaaagat actggtgttc ttatcggtac aaattctaaa
     5041 gtatatcaag aagtgaaaga aaaaattgaa aatcaactca atccctcccg acgaaatcaa
     5101 cccaaaaatc gtcaaaaaga aaccaatcaa acattcactt ctagaaattt tgacccaaca
     5161 aactttctaa aaagatgaaa aagaaaccga aaattttttc tatttctcag tttctttttt
     5221 agggggcagt cttccagcaa gatttgcaag ttaccggtaa cttgcacaaa aaaagaaaaa
     5281 tccgcttgtg cgaatccttc tttttgcatt ttggggcgct gccccaatct ccaaatatcg
     5341 gttatttaat agcgtggtaa ctcaaaaaaa tagaacgtac tctgaaataa aaaaagactg
     5401 ctctaacgag cagccaataa aaaataatga gcgtttattc ttgaaaaagc agcgcgctta
     5461 tgaagcgctg ccaaaaattt accatatttt atgaaaaata acaagtttct aaaaaggaat
     5521 cggttaagat tcacaaagaa tcgtaaacag acaatagcgg aaaatcaagc ttccgaacgt
     5581 accttttagc tagaccaatg aaaaatcaca aagtgagcgg ctggatcggt tggtacaggg
     5641 gctacttggc atagtcgaca ttatgtctac tagctcgata aataacgatt atgttcccaa
     5701 aacaatattt tatgaactat aacaaaaatt tgttatcgca tattgtatat tttaattata
     5761 ggtgtatatc a
//
LOCUS       DAMEET010000116         5692 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.119, whole genome shotgun sequence.
ACCESSION   DAMEET010000116 DAMEET010000000
VERSION     DAMEET010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.119"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(72..542)
                     /locus_tag="PB780_002373"
     CDS             complement(72..542)
                     /locus_tag="PB780_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HDE0734396.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            872..2512
                     /locus_tag="PB780_002374"
     CDS             872..2512
                     /locus_tag="PB780_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HDE0734397.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2666..3988
                     /locus_tag="PB780_002375"
     CDS             2666..3988
                     /locus_tag="PB780_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HDE0734398.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(4056..4490)
                     /locus_tag="PB780_002376"
     CDS             complement(4056..4490)
                     /locus_tag="PB780_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDE0734399.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(4555..5391)
                     /locus_tag="PB780_002377"
     CDS             complement(4555..5391)
                     /locus_tag="PB780_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDE0734400.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
BASE COUNT         1705 a         1136 c         1043 g         1808 t
ORIGIN      
        1 gcaggttttt cacgacctct gattcacggt gtttcttttt catcaacatt tttacgaaat
       61 actgtggaag actattgaaa taagtgtgga ttgtttcttt gtgtttcaat cgtttcttgc
      121 cattcttcat ttaattttgg attaccgttt tcttccttga actcataagg tcctaattca
      181 aaaatcccag acagtatttg attcacgatt tcatatagtt cagtgtgatc agacacatga
      241 ttcttgaaga tcatctcatt atcaccacgt ttgaacctca cattcgggct ctcatgatga
      301 acctctatgc atttttctcc tttttttaac tgacggactt ctgaaaacat ctcataaata
      361 tcttctccag taaaaccttc agcaagcgcc agactgtcca caagaccgcc tactgtcagt
      421 tcagagatta ttgcttgatt ggaatctaat tttaatgtat agcttgtcgc ttctactacg
      481 ccacatgcat tgcaaccgcc ttctgatagt tcgctttttc taatcaattg aacttctttc
      541 attttctctt cctcctctta attttctact tatgtatttt tatcacctga cataggtgac
      601 tcctatcaac gctatcatgt gcttttttcc tcagaaatcc tatttatcca ttccatctat
      661 cccttattct tctacttttc tttctctagc atataagaga tacgcatagt attcaagcaa
      721 ggattgtcga tataaaaaga acaaatactg ctattttgat ttcttttggc tatattacat
      781 ataaataaaa tttatcatac ctgctctttt tgactatatc atcatctgac ttctatactt
      841 cgtttgtatt aaaacttagg aggaatcatt catgcttaca gggttaaact tattaaataa
      901 tccttttcta aataaaggaa cagcttttac aaaagaagaa cgggcaaaat atggaatcac
      961 tggtatgctt ccaagtactg tccaaacact ggaacaacaa tctgttcaag cttatggtca
     1021 atacttaagc aaacaaacag atttggaaaa acgtatcttc ttaatgaatc tattcaatac
     1081 gaatcggaca ttgttttata aattgatggg cgaacattta gttgaattca tgcctgtcgt
     1141 ttatgatcct gtcgttgcgg attcaatcga acaatacaac gaaattttct tgaaaccgca
     1201 agatgctgca tttctttcaa tcgacgaacc agaaaacatc aaagcaagtt tgaagaacgc
     1261 ggcagatgga cgagacattc gtttgattgt tgtgactgat gcagaaggaa tcttaggtat
     1321 gggcgactgg ggtgtgaacg gtgttgacat cgctatcggc aaattgatgg tctatactgc
     1381 tgctgctggt attaatccag cacaagtttt gcctgtatcg atcgacgctg gtactaataa
     1441 tgaagaatta ttgaataatc ccttatactt aggcaatcgt catgctcgtg tagaaggcga
     1501 aacttactat gaattcatcg atcaattcgt tcaatcagct accgaattat tcccagaatt
     1561 gttattgcat tgggaagatt ttggacgagg aaatgctgca gcgatcttag aaaaatacga
     1621 agatcaaatc actactttca acgacgatat ccaaggtaca ggaattgttg tcctagcagg
     1681 tgtattaggc ggattgaata tatccggtga acaattgaaa gatcaaataa ttttgacatt
     1741 tggtgctggt actgctggtg tgggtatcgc aaacatcctt ttagatgaga tgatccgtca
     1801 aggtgtacct gaagaagaag cacgccaaca tttctatcaa gtagataaac aaggtttatt
     1861 gtttgaagat acagaaggat tgactcctgg tcaaatccca ttcgctcgca aacgctcaga
     1921 tttcactaat gctgatgaat tgacaaactt agaagcagtt gtaaaagcga tccatccgac
     1981 aatcatgatt ggtacttcta cccaacctgg agcattcact gaagaaatcg tgaaagaaat
     2041 ggctgctcat acaccacgtc cgatcatcat gcctttgtct aatccaacaa aattggcaga
     2101 agcaaaagca aaagatttaa tagaatggac ggacggaaaa gcattagttg gaacaggtat
     2161 ccctgctgct gatgttgaat ataatggtgt aacttaccaa atcggtcaag caaataatgc
     2221 tttgatgtac ccaggtttag gacttggttt gattgcttca acagctacac gagtaaatgc
     2281 tgaaatcatt tctcaagcta gtcgagcatt aggcggaatc gttgatgtaa caaaaccagg
     2341 agcagctatc ttgcctccag tagcaaaaat cactgaattt tctcaaacga ttgctgaaac
     2401 tgtcgcaaaa tcagttgtcg cacaaaactt gaatcgtgaa gaaatcacgg atatcaaaga
     2461 agcagttgaa tctgctaaat gggttcctga atataaatct ttagaagatt aagatcgtta
     2521 tagtaaaaga acatacagaa actttgtatg acgtctaaaa agaaaaaagc gactgcggca
     2581 aaacagcggc cagccaactc ctgctctttt gtcgcttttc tttggctttt ttcatatgat
     2641 acagaataag aaaggatctg taaaaatggc acaaaaaaca acaactgcaa ctgcagttga
     2701 ggaaaagaaa aaagaaggtt tttggtccac aaaaatcacc ggcgtaagtt tgccacttta
     2761 tttgatcatg gtccttgtcc tgatcattgc aatggcttta gggaaattac cgacgaatat
     2821 gattggacca atcgcagttt tggttatttt cggtaattta tttcatttta tcgggacaaa
     2881 aatccctatc gtcaaaagtt atttgggtgg tggttccgtt tttgcgatct tcgcttctgc
     2941 tgcgattgct acatttggga tcttacctga atatgtagtg aaatccacag aaaactttgt
     3001 cagcaatatg ggcttttggg acttttatat tgctgccttg attactggta gcatccttgg
     3061 tatgaaccgt aatctattga tgaaagcttc tgttcgtttt attcccgttg cacttatttc
     3121 aatggtcgtc tccttttttg ccgtaggact cgtgggcatg cttatcggaa atggttttgc
     3181 aaattccgtt ttatacatct cattacctgc gatggctggt ggtgttggag ccggagccgt
     3241 tcctttatca acgatttatg caaatgtttt aggtacggat gcttcttcta tcatttcccg
     3301 cctgattcct gcgacagcaa tgacaaatgt cttagctatc atcggtgcag cattagttgc
     3361 tcgtgctgga caatcaatgc caaaattcaa cggcaatggt aaattaatgg aaaaaggcga
     3421 tcttggcgat ggaaaacctc gaactgtgaa accttccatt ccacagttag gtgttggttt
     3481 gatggtatca ttgactttct tcatcttagg acaaattggt aactattttg ttcctaaagt
     3541 ccatgcttac gcatttatga ttattatcgt tgtcatctgt aaaatcgcca acctattacc
     3601 agagtattat gaagatgcag ctatcatgtt caataacttg attgtcaaaa acttgacagc
     3661 agctgttctt gccggtatcg gtattgcgtt gctgaatctg aatgtattag cttctgcttt
     3721 gacatggcaa tttgttgtct tatgtctgac aagtgttatc gcgatttcag ttgtttcagg
     3781 atttgtcggt cgtttgtttg gtttatatcc aattgaatca actattactg ctggcttatg
     3841 caacaactcg atgggcggaa caggaaatgt agccgtctta tctgcagcta atcggatgga
     3901 acttattgct tttgctcaaa tgggcaatcg acttggtggt gccatcgttt tgatcatctc
     3961 tggtttcttg atgcaattac tttcttagtc aaaaaaacaa aaatacctga tcatcaagta
     4021 tcggcttggt gatcgggtat ttttttgata aagatttaca ggattttcaa aaatcttttt
     4081 tgcattttat cggaatgata accgatgtgt tcataaaatt ggtgtgcttc tactctgtat
     4141 tctgcagaat taagccttat tgtcgttatt cctcgttttt tcgcttcttc ttctacgcca
     4201 gtcatcaaag cctttccgat gcctcttttt tcagcatatt tagacacagc aagtgccata
     4261 atattgaaca ttggatcgct aaaaatcgat tcataggact ccgcttgtac atagccagct
     4321 gcttttttag aagtaggatc ttcataaacc aagaaaaaat ttttctctgg ctccttcagc
     4381 agtttcttca tttgtctcac cgtaacggtt aatggtatat cataccctaa ttgatccgca
     4441 ttgatttcac gtagtgcttc tgcatcttct aaacgcattt cccgtaacat ccaaatcacc
     4501 agccaatttc tgatttttta aaatgaattt ttttatcata gtatactctt tttttcattt
     4561 agaacaatat gtttcagtga ttttgacttg cgttagtcta gattctccta atgtttgtaa
     4621 gatgacaaag gcattgtaac aattgaccaa aagaaccacg aggacaatta cttgaaaaat
     4681 aaaggtcact ttttttggag acaaaaccct gctaaatctt gctccccaca aacctccaat
     4741 aacagcagcg gggataacat aaaacaacat agataaatca aagcgcataa agctacttgt
     4801 taaagcaatc gttaccaatt ttgccagttg agaaaagaaa atcgtagata gagaatatag
     4861 tgttgcttct tttattggca atgcaaacat cagcatcaac aaagaaacat tgactggtcc
     4921 accaccaata cctaaaaaac tagctaaaaa gcccagaacc cccccacata taaggtacca
     4981 agtccatgac gtcaaatgaa attttggctg atgatgcttg gtgtagaaga acgcaaaaac
     5041 caaggtcaat accgtcaaaa aaatttggat cagctgcaca tgcttctcgt tttcaaataa
     5101 ctgtaaaaaa acttcgaata caatgttgcc gactactcct ccaacaacag ctccactaga
     5161 tacccaaccg acaatctgcc aattcagtga tttcccgctt gatacttgtc gcacagtcga
     5221 aacaatggac atcgtgaata ccgcaactgt tgagtaaaaa gaaatccctg caacagaatg
     5281 ggcgccgata agatcaagaa tcggtttgat caacacgcct ccacccatcc cggaaatagc
     5341 gccaactgtg ttcgccagaa cgatcaccac aaaataaatc agaccaatca tcttgtattt
     5401 cctttctctt gtttatatct tttagcgatt gttactcttt tcttttatta tacagtaaaa
     5461 gaaacaaacg aggcttcgaa agcttttcta ctttttaagg aaatttagtg ctaaacgaaa
     5521 agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagctc
     5581 ctcataattt tcgtttggtt gaacttatta ccgcagatgc aagcttttga acaccgttta
     5641 agaaagaggt cgtgagcctg ccgttcagct ccgagtcaca aggaacaatt tc
//
LOCUS       DAMEET010000117         5689 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.120, whole genome shotgun sequence.
ACCESSION   DAMEET010000117 DAMEET010000000
VERSION     DAMEET010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.120"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(488..1297)
                     /locus_tag="PB780_002378"
     CDS             complement(488..1297)
                     /locus_tag="PB780_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDE0734401.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1297..2043)
                     /locus_tag="PB780_002379"
     CDS             complement(1297..2043)
                     /locus_tag="PB780_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDE0734402.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2061..2528)
                     /locus_tag="PB780_002380"
     CDS             complement(2061..2528)
                     /locus_tag="PB780_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDE0734403.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2528..2938)
                     /locus_tag="PB780_002381"
     CDS             complement(2528..2938)
                     /locus_tag="PB780_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDE0734404.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2949..3962)
                     /locus_tag="PB780_002382"
     CDS             complement(2949..3962)
                     /locus_tag="PB780_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDE0734405.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4179..4913
                     /locus_tag="PB780_002383"
     CDS             4179..4913
                     /locus_tag="PB780_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDE0734406.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4897..5607
                     /locus_tag="PB780_002384"
     CDS             4897..5607
                     /locus_tag="PB780_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDE0734407.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSGSVAKF"
BASE COUNT         1832 a         1195 c          943 g         1719 t
ORIGIN      
        1 aagcgacaaa aaccacgagc aggtccattc atcctcaaac ctcggtgatc ttcgcggttt
       61 aacaccatca gaagaaatgc acaaaaataa taaggagagt ctgagacata aataaattcc
      121 cagactttta gtgagtctaa atccgaataa acggtgggtc gaaagcaact ctagctgcgc
      181 gtcgatcgca gcaatctcta ggtgctatgt tcggataaat ggtggtcaaa agtcagattt
      241 tcggccattt cgtaacagcc aaccagacaa aaagcgtctg ttttgtcttc tactccaatt
      301 gctcaaatct tagcgacttt tggcttggtc ttactctaaa cagtttgtaa gcctaaactt
      361 tatttgctgt ttgtttctaa gatcaaagca aaactacttt ttagttggtt acttttacaa
      421 gttcttttta ctgttgaata aatgtaaatc ttaagaacaa atatttactt ctataattcc
      481 aagtttctta aaaaattccg ccaagtgtac ctaaaaagca taaaacaaaa attcctagaa
      541 taattgtgat cgttttatgc cctttggagt aaagccagac gcataaaaag gtaattccta
      601 atggcagcaa gcccggaaaa atttgatcaa aaatactttg taatgaagtt tcagctccac
      661 caaccgtgaa ttttagtgac gtatttaaag taaccatcgt gcctatcatc gcgccaatta
      721 ccgttaaacc taaaatcgaa gcaccatgag ataatttgtc aatcatatgg ttgtcttctg
      781 tatcatcaat aaatttagtt cctaaaacat agccatattt tgtcgccaac catttaaaaa
      841 taaaatgaat ggcagtatac aaagtaaaat aaaggatcat gcctaaaata ttcccttgtt
      901 gagcaaaaga aatacctatg ccagccgcaa taacccgaaa agttccccag aaaaatgaat
      961 cgccaatacc tgataaaggt cccattaatc ctgttttgat tttattaatt gcgtctgtat
     1021 caaattcagg atttttagca ttttcttctt ccatggtgac ggacaagctc ataacaaaag
     1081 gcgcaatatg cggtgtaatg ttaaatgttt ccgtatggcg ctgcaacgct gtataataat
     1141 catcatcgtt cggatatatt ttacgtaagg aacgttcaat accattcatt tgtccatatg
     1201 ccatcatttt atcgaaggac caagaggatt gcataaaaaa ggagcgccaa aatatacttc
     1261 ttaattcttt tttcccaata acactttctt ttaccattat aaatcttcct cctcttcaaa
     1321 agttagcttt tgaacagcca gtaacgctaa gcaaagtccg acgacagcca caccgacaac
     1381 atccattttc agatacatta cgccgacaaa tcctaagaaa agataaggca tcatcgcttt
     1441 attcgccatc attttaatta ataacgctag cccaacacaa gaaagcacac cggccgcaac
     1501 atcaaagctg tccaatacaa cctgaggaat caacttgatc atcgcattga ttgtagacac
     1561 accaaaataa gtagctacaa aaactaaaat ggccgatgga accccaacga ttaaaggttg
     1621 agccacataa tgatattttt gtgctttttt gaaatcatga ttgactaatg cctcatccat
     1681 tttgttgtta aaatacgtaa ttggcattgc ataggcgaag ttacgccaca tctgaagaaa
     1741 gatggacaaa ggtaaagtca acgcaacggc agttcctact ccttgacccg attgaatcgc
     1801 aattgctgta cctaaaacac tggaagcata aacttcaggg ggcgtcgcac tccctaccat
     1861 aattgagccc atgaacatgg attccaaggt ggcaccaata ataattcctg actctaaatc
     1921 tcccaaaatc aaaccgacaa aaggcgctgt aaatagcggt ctacccatca tcgatgcacc
     1981 aaatgtgtat tcatcaatag acgccaaaaa agctaccagt gcgattaata ttgcttgtat
     2041 catattcttc ccctcctctt ttagtcatgc aaaggattga tttttttgtc cgttggtaca
     2101 gcctgacatt gaactgttat cccgttttca gtcatttttt tcaccaagtc cagttcttgt
     2161 tcattcaagt aaacggaact agtaatcatt ttccgattct cgtttttctt taaatttcct
     2221 aaattcactt ctttaatctc aggtacgtgc tgagtaattt cgtaggcatc ctccaccgtc
     2281 ccgacaatta aaaatacttg gtatttcttg attttttctc catttaaaac agagatggcc
     2341 tctgtcatat ccttcaccac aaattttgtc cctgtaggaa cagccatttt tagtgttttt
     2401 ttgcgcagat tattggtagc gatttcatta cttgcaacga aaatacaatt tgcacctaag
     2461 ttatttgtcc aggcaaaagc aacttgccca tgaacaaaac gttcatctac tcgtaataat
     2521 ttaatcatta aaaatcatcc tcttctgttt tctctagcaa ctcgttcacc aaaactatcc
     2581 cttcattccc gctattcata gctttttgga ttagtttttt cgtgtcctgt tcacctgata
     2641 agcaaagctc taaaagcagc ggcagattta caccgctgat taaataaaaa ttgtctttac
     2701 cgatttgttc ggagaaagcg ttagacacgc ttcctcccag taaatctacc gtcacaatta
     2761 attcgtcatc aggcgctaag tcggaaaata gctgctctat ttcctggtta atggagtagt
     2821 cactatctat atacgcagtt attgctaaaa gtgatttttg aggaccaata ataaattcta
     2881 aagtatcctt taagccttta gccatcatgg aatgcgtcgc aattacaatt ttcctcatag
     2941 ctcttacctt aatattccac agtccgataa taacgacgaa tatctagtga atgatcccgc
     3001 accttttcaa aatgagcact cactcgttct aaggccgttg ccataaccat tggcgctaca
     3061 tactgacgca tagcgcttgg tacacctgct aattcgaaat ccgcagtgtc aaaaaccgtc
     3121 acaacatcgc tatatttatt tacaaaacgc tctacgcgat cgactagcgg acgcgtttga
     3181 tcttcacctt ttaataacac aaaactcata gatttatcag tcatttcaat agtcccatgg
     3241 aaaaattcgg ctgcatggat cgacttggtg gcaatccatt gcatttcttc cagtacgcac
     3301 atagcatatt cataggcagc cccataagta ttcccggctc caacaaacat atgaaattcc
     3361 gtatttttat gtttttctgc aaattcaagt gctttctgat cattttgttg ccgaacactt
     3421 agtaatactt ccggcatttt ttgtaatgtt tgaataaatt catcatattc aggaaactca
     3481 ccgttatttt tcatcaggcg tgcacccaaa gcaaaaaact gtaactgaag ttcttctact
     3541 aatgtatcgt tgtcagcatc attttcaatc gcataatcca cgatttcccc gagtggtgta
     3601 gcagcatttc ctacaaacgc aatcgtcgtt gcaccttgac tattggcata cttaacaaca
     3661 tcaacggttt cttgagtcgt accggttaat gaagatgtga ttaacactgt atctttatta
     3721 atctttttcg gctgtgaaca aaccacctct gcggctattt catgataata atcaagctct
     3781 gaatgagtgc ttaacagata agctagcggg ctgattgtgg catatgttcc accagagcct
     3841 accaaataaa gatttttaaa tcccttctca ctgattgcat ctgccacctg attgattgtc
     3901 tcgatatttt ttactgcacc atttaatact gatagaaaac gttcttcgct aaaacctaac
     3961 atagttaaaa acctccaagt caataatttg aaaaatatat ttgcaaatgc cggcacatta
     4021 acaaaccact taaccttttt tatataccac ctgttaacgt ttttaatgta ccacttatag
     4081 aaaacgctgt caatagtttt taaccacttt tatttttatt tgtaccaaaa tccttttttc
     4141 atgtataata aacaaaaata gactctggag tgtaacgtat gaatactgaa gaactaacga
     4201 acaaattaat acaagacatt gaacaagaaa aatataaata cgcgcaagct attccttcag
     4261 aacgtgattt tgttcaaatt tatgaaatga atcgttcagc cgtaaaaaaa gccattcaac
     4321 aattagtaaa taaagagtac ctactaaaaa tacatggcaa aggaactttt gttaaaaaaa
     4381 atatccatga acatttgcaa atcaaatttc atggtttgtc tgaattgttg gaacgggaag
     4441 ggattcagcc tagcagctta attttagctt cagaaataaa accggctgga cacattgtcg
     4501 ggaaagcttt acagctggaa ccaactgaag aagtatttca tttggttcgt ttaagaagcg
     4561 gcgacggtga gccaatttct attgaagaca cttatattcc attaaatctc atacctgatc
     4621 ttcaagcttt tgatttccaa attttttcat tttattccac attggctgct tatcattatc
     4681 aaattgaaaa tatctatcag acaatttcca gctcaaaagt gagcaataaa gaagcccgtt
     4741 tgttaaaaac cagcttaaat cgtccggtaa tcagccttgc tatcactgcg gttacgacaa
     4801 atgaacaagc ggttgaatac actgaagtag tggttttgcc tgaattcgcc gccatctata
     4861 ctaaaaatgt tcatcaagga aaggagatga aaatctatgc ccaaattgat taatctgttg
     4921 gcttatgacc tgattgacaa aattattgct tatattcatg aacatcattt aacccccgga
     4981 acaaaactgc cttcggaacg ggacctatca gaaatgttta atgttaatcg aactacctta
     5041 agaggcgcgt tacaaaaact ggtggacaac ggcaccttac gggcgcaaac caatagcggc
     5101 tattatttaa ctgcgccaaa attcgaaaaa aatgcccatc atttctttac accgatagaa
     5161 gattctctgc ttagtaagca gcggctacag ctaaaggcaa cggctctttc accgaaaaaa
     5221 gcagcagctc tttgtccgga actgaatgat ttcaaacagc agcaaccaca agcttattta
     5281 gaatatattg atgcagaagc aattagcttg atgttattaa tccctatgga aacattaaag
     5341 aaaagtaatt tctcggacaa tcctttccaa gattttaaaa gtttggcata tacgcgttct
     5401 caagaaattt ctttaataac tgacaatgac gttgcggcgg acttgctggg agttgacaaa
     5461 gaagaggttc tgttatttgt acgagaggaa ataagagccg gtaaggagtt ggcggcgatt
     5521 gctttaggtt atactattag tcgccgctgc aacatacagg ttaatgttgc tacgaccttg
     5581 caatccggtt ctgttgcaaa gttttaaatc tactatcaga taaggtagaa taatagaaaa
     5641 agatagcagg aggaatgacg atgagtcatt ttaaaggaaa gcaatttca
//
LOCUS       DAMEET010000118         5601 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.121, whole genome shotgun sequence.
ACCESSION   DAMEET010000118 DAMEET010000000
VERSION     DAMEET010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.121"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            65..766
                     /locus_tag="PB780_002385"
     CDS             65..766
                     /locus_tag="PB780_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HDE0734408.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            763..1884
                     /locus_tag="PB780_002386"
     CDS             763..1884
                     /locus_tag="PB780_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDE0734409.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1899..3131
                     /gene="pepT"
                     /locus_tag="PB780_002387"
     CDS             1899..3131
                     /gene="pepT"
                     /locus_tag="PB780_002387"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDE0734410.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3263..4171
                     /locus_tag="PB780_002388"
     CDS             3263..4171
                     /locus_tag="PB780_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HDE0734411.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4206..4478
                     /locus_tag="PB780_002389"
     CDS             4206..4478
                     /locus_tag="PB780_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDE0734412.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4489..5535
                     /locus_tag="PB780_002390"
     CDS             4489..5535
                     /locus_tag="PB780_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDE0734413.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         1821 a          972 c         1216 g         1592 t
ORIGIN      
        1 tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta gaaggagtga
       61 agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact ttgtccctga
      121 atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg cattgatatt
      181 aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt atgaatcagc
      241 acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc ttgcaaatgg
      301 tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg gtatgggagg
      361 ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa gcggacaaga
      421 acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc tgcaagaaaa
      481 tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat atgaaatcat
      541 tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga tgttcgggcc
      601 atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag agcttcaaca
      661 aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac gtcaaaaaca
      721 aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat cagcggacgc
      781 actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga aggagaccca
      841 gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat gacattagac
      901 gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct gattgctaaa
      961 catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca agaaaaaatg
     1021 tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa catggatatt
     1081 atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt ggagagttat
     1141 ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc tgtggatcat
     1201 gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg tgattatacc
     1261 ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg tgcacaacca
     1321 gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga ggttattttg
     1381 ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc ttatgaggaa
     1441 cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt aggacgagta
     1501 ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa agaagcgttc
     1561 caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt gaagcgcatt
     1621 gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca aagagcagat
     1681 gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag cgctgggttg
     1741 attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt tatcgagaaa
     1801 tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc tgaaacaaat
     1861 acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat cttttgccac
     1921 gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa acgactccat
     1981 cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa gagttaggct
     2041 tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta ccaagcaatg
     2101 ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca gattttaatg
     2161 cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg atcgtactag
     2221 atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa aattatgaag
     2281 gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac aaatcaggga
     2341 ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc cctcacggta
     2401 ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat aaattcgatg
     2461 ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta ggtgagttgc
     2521 agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa aatgtacatc
     2581 cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat tttcataatc
     2641 agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc ttccatttga
     2701 tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc gatcatgatc
     2761 gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag ttgaatcagc
     2821 gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac atgaaagaaa
     2881 tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc gagcttggta
     2941 tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc tcttacatgg
     3001 gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga ttcgaatttg
     3061 tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca gaattgaaca
     3121 caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca atgatggaat
     3181 gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc ctcttttttg
     3241 aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca aaagttcagg
     3301 gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag aaaagcagcc
     3361 agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg aattagtcaa
     3421 gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg gcacgattca
     3481 tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat tacttccagg
     3541 tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag aagaagctgc
     3601 tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca atgatgaagt
     3661 cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga tcagtcaaga
     3721 agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt tattgctgaa
     3781 gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag gaaaatccca
     3841 actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg acgactcagc
     3901 gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata ttttccgatt
     3961 tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg gcattactta
     4021 tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca ctcgaacgga
     4081 tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag gattaacagt
     4141 ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa gttggaagaa
     4201 gagtaatgga tgtattgtta cacaatcctt tgtggtttta ccttttcatt gtgaattttt
     4261 acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc tggcgtgttc
     4321 ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga ttgatctctc
     4381 gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc gctgcaggtg
     4441 tacttgttga tttgattctc atttattttt tccattaagg agcaggagat gtcgtctaaa
     4501 ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt tgccattatt
     4561 tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc taccagtgtt
     4621 gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga agggcgctca
     4681 atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg gttcttgacc
     4741 tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct gatctcggag
     4801 atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt gacagcactc
     4861 catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt ggtcatccaa
     4921 ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc tggtatcctt
     4981 ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa tgttcaaata
     5041 ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa atgggtggat
     5101 acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat ccaagacagg
     5161 actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg gtatgtactt
     5221 ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac catttcgttt
     5281 atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat tggttccttt
     5341 gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc agtttcttcg
     5401 cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt tgtgtatgtg
     5461 attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa agagaataaa
     5521 gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa agaaaaacgt
     5581 tatggataag ataattttac g
//
LOCUS       DAMEET010000119         5524 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.123, whole genome shotgun sequence.
ACCESSION   DAMEET010000119 DAMEET010000000
VERSION     DAMEET010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.123"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            84..168
                     /locus_tag="PB780_002391"
     tRNA            84..168
                     /locus_tag="PB780_002391"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:118..120,aa:Leu,seq:caa)
     gene            423..1298
                     /locus_tag="PB780_002392"
     CDS             423..1298
                     /locus_tag="PB780_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDE0734414.1"
                     /translation="MKESASCFVLSYSGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAIEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            1402..2166
                     /locus_tag="PB780_002393"
     CDS             1402..2166
                     /locus_tag="PB780_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HDE0734415.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            2153..3595
                     /locus_tag="PB780_002394"
     CDS             2153..3595
                     /locus_tag="PB780_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDE0734416.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAIFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            3606..4936
                     /locus_tag="PB780_002395"
                     /pseudo
     CDS             3606..4936
                     /locus_tag="PB780_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
     gene            4949..5407
                     /locus_tag="PB780_002396"
     CDS             4949..5407
                     /locus_tag="PB780_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDE0734417.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
BASE COUNT         1854 a          821 c         1164 g         1685 t
ORIGIN      
        1 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
       61 ccccagttat tatttttaaa tttgccggcg tggcggaatt ggcagacgcg ctggactcaa
      121 aatccagtgt ccgttgagga cgtgccggtt cgaccccggc cgccggtata attatcaata
      181 tagcaacttt cttatagaga gttgctatat tttttttgtt tttatgtaat tacctccaaa
      241 tcaattcgat acaagtatta tcataaaacg agtaaaaaag atattgataa cgcttttatt
      301 atgtgttaaa ctatttttgt aaattatgta gggtgctact attcaattag gcacgaccta
      361 agaaagggtt ctttcttagg tcgttttttg tctttaaaaa ttgtttggag acaaaaatat
      421 gtatgaagga gtctgcaagt tgttttgttt tatcatatag tggaggtgaa ttaacggtgt
      481 atgtgatgaa aaagctgaat cataatgctg tattgatcga tgacgatgga atagaaaaaa
      541 ttgcgatggg gaaagggatt ggcttttctg ctaaagtgag taagccattt gatgttgagg
      601 atgctgacaa attatttgta ttagattcta aagaaaagat caaacgattc agtgagatag
      661 tctcgcagat tccaatcgaa tttatggatt ttgcggaaga agtgattgaa ctgataacag
      721 ctaggatctc ctctccatta gattcaaata tctatattac attgacagat catatttatt
      781 ttgctattca acgaaaaaga gaagatagag atgtcacagc aattatgttg ccagaaatga
      841 gattccttta tccaaatgaa tatgatatag caatagaagt ggtcgagcga atcaatcatc
      901 gatatcaaac cgagttaggg tcaaatgaag taggattcat cactatgcat atcattaatg
      961 ctgagctagg agagcctaat agtttaaaga gcttaaaaat agtagaaatg acgaccacga
     1021 ttttgtccca tctagaaaaa tcctattcaa acgtttatga taaagaatca ctgacttatc
     1081 atcgcttaat ggttcatatc aaattcctgg ttaaacgctt gatttatcag gaagaaacgc
     1141 cagtagaaaa tattggtttt tttactgaaa catttaaaca tagcaaagcg tattgtacag
     1201 cactaaagat taaagatatc atcgaacagc aatttcaact tgccattcaa gaaaatgaaa
     1261 tcatctattt ggcaattcat ctagcacgaa tctattaaaa taagggcgta actatctata
     1321 ggcgcaacca aatgacacat agtaaatgga ctatgattat ttggttgcgc ctattttagt
     1381 agaaaaaaga aggagaatga aatgggaaaa gtgattttta attcggatga ttttggctat
     1441 agccatggtg tgaattatgg gattatggat gcttatcagc gaggaatttt gacatcaact
     1501 acattgatgg caaatatgcc gggtttcgag cacgctgtga aactaagaaa ggaaatgcct
     1561 aggctaggtg taggagtcca tttaacattg acgtgtggca aaccacttct caaaactgtc
     1621 gatactctta tcgaaagcgg agagttcaga tccttatctt attatcagca tccatttaac
     1681 attgacaaag atcaactgta tcaggaatgg aatgctcaga ttcaaaaaat ttatcatgcg
     1741 gggatcatac caactcattt agacagtcat catcatactc atactttgga tgaaaaccag
     1801 gaggtagttg ttacattagc aaaaaaatat gatttgccgg ttcggagcaa ctttgaacga
     1861 aaagatgaag ttcgtcatgt cgattatttt gagccttgtt tcgatgtcgt aggagaaact
     1921 gatgaaaaaa aacgacaaat caatacgagt gtggaagaat atttggcaag tttactcatg
     1981 atattgagag aagataaaac gatcgaagtc atgtgtcacg cagcgtattt ggatcaggaa
     2041 ttgttaaatg gttcaagttt tgtgtatcct cgtactcacc aagtcgaatt tttaattcat
     2101 tcagcatttg ctcaaaaagt gaaaaatgat cctttgattg agttagtaac atatgaggat
     2161 atttaataga ggaggaataa aaatgaatgt taaatcaaat gtgcagaagc ttggaagagc
     2221 gatgctactt ccagttgcag cgatgccatt agcgggattg atcatgcgtt tatcagctga
     2281 tgatatgctg aacatcccag taattggtgc agctggaaat gccgtttttg gaaacttgga
     2341 tatcttattt gctataggag ttgcaatcgg ttttgtcaaa aataaggaca aaggaatacc
     2401 tgcacttact ggttttttag cgattgctac cttgaaaaaa ggcttagaaa tcatggaccc
     2461 agaagtgaat atggggattt ttggtggaat tatttccggt ttagtagcgg catggacgta
     2521 taatcgcttt aaagaacaaa aattaccagt agttttttct tattttgcgg gcgaaaaatt
     2581 cccattgact atggtgatga ttctacaaac aatcgcagct atttttttgg gctttatctg
     2641 gccgctcatc caaattggga ttgataattt tgcaaaactg ctcgttgata tgggggcatt
     2701 aggtgtgggg atatttatgt ttttgaatcg tttgctgatc ccctttggac tgcaccatgt
     2761 gttgaatacg tatatttatt acgatttagg aagctataca tctcctagca aggaagtttt
     2821 cagaggagag atgacacgct ttatcaacgg agatcctcag gctggactgt ttttatcagg
     2881 tttttttgta gtaatgatgt ttggtatacc agctatatgt ctagctatct ataaagcagc
     2941 ttataaagaa aataaagaga tggtcaaagg aatcatggga agcggtgcag gaacttcatt
     3001 tattgcaaat atcactgagc ctgttgaatt tagtttcatg tttctttctc ctatgttgta
     3061 cgtgatccat gcactttttg ccggtttagc agggattgtc tgttacttat tcaatatacg
     3121 aattggtttt acattcggag catgtattgt tgattatctc atcaattttc gaatcgctac
     3181 caatgctatt ttgattcttc caatcggaat tttctttttt gccttgtatt acgttacttt
     3241 ttattatttg atcaataaga gaaacattca gacattagga cgtgaagcta aggcagagtt
     3301 tggcaatgaa gtaaccttag aagaaacaga gttagggtta gcaagtaaaa attatgaata
     3361 tatggcaaca aaaatgctac aagcgtttgg aggaaaagcc aatatcctgg atgtctatag
     3421 ttgtaataca cgcctacggg tagaggtagt ggatcccacc atggtcgagg aacagcgaat
     3481 caagcaatta gggataagcg ggatcattaa accaactgaa aaaaattatc aaattatcat
     3541 cggcttagaa gtgacttatg ttatggcaga attcaataaa ttattagaag aatagaaagg
     3601 aaaagatgat gatgaaaaaa cagattatca ctatagctgg aggcgggagt acgtatacac
     3661 caggaatcgt tcaagccatt ttgaacaatg aaaaacggtt gccattatct gaaatacgtt
     3721 tatatgatat tgatgaagaa agaaatatgg atatgtactt gattgttaaa tttatgctga
     3781 aaaaaaaggt ttttcaaata ttcgcattcg tgcaacggat gatccaaagc ttgcttttac
     3841 aggatgcgat tttgttttct cacaaattcg tgttggagga ttggagatgc gagaaaagga
     3901 cgaaaagatt ccacttaaac atggattagt aggacaggaa acgtgtggac tcggaggatt
     3961 tgcttatggg atgcgctcaa tcaaaggttt attggaaatt gtcgatcata tacaagacta
     4021 tgcccccaaa gcatggattt tgaattatac aaatccagag tcgatcgttt ctgaagctgt
     4081 tcgtcgtaaa tatccgaatg taaaaatgat taatgcctgt gatatgacta tttcaattga
     4141 agaaacgatt gcagtgaatt acggttatga tcgtaagaat tggatcgtta cgtattatgg
     4201 tttaaaccac tttggctggt atacatcgat ttatgataaa gaattgcaac gagaaattat
     4261 gccagagatt attgaaaaac taataacaaa agaaatgcaa gtcgccgatt ttaatatagg
     4321 agacaaaact tggcaaaaaa cctttcaaat gatgtcagtc atcacgaaaa attttccaag
     4381 taatatcccg aataattact tagagtatta cttatatcct gatatggtcg ttgagcatac
     4441 tgacaaagag tatacacgtg ctaatatggt catggatgga cgagagaaaa ataccaaaga
     4501 aatggctgat aaaatacgcc gcggtattgt ggaagaagta ttgaacttta attttgggga
     4561 gcatggacaa tatatcgtcg atatagctat ttccttgttg aatgatgatc gtcgtcgctt
     4621 tatgctaatc gttcctaatc aaggggcgat tccgaatcta cggtcagatg cagttgttga
     4681 aataccggca tatgttggtg caactggtgt ggagacaatt acactaagaa aaccaatcag
     4741 tgattttcat aaaggattga tggaggcaca agttgcagca gagaagttac tagtagatgc
     4801 ttactttgaa ggttcttatc aaaaagcttt acaagcattt acattgaatc aaaccgtacc
     4861 aaatgccaga gtcgcaaaaa aaatactaga tgaaatgatc aaagtaaaca aaaaatattg
     4921 gccagcatta aagtagaggt gataaaaagt gcatttcttt aaaacaagaa acattctaaa
     4981 agctgtagct agtggtcaaa ttattccatt aacagcagta aaagacgagg tcttttcttc
     5041 aaaaatgata ggagttggat acgctataac gaatcatgat ggatatatcc atgcgccagt
     5101 taaaggaata atcgataata tatttcctac ccgacatgcc ataacgatcg aaagtgaatc
     5161 tggcagaaaa atactgatcc atatgggttt agatacagtt gaactcgaag gaagtccgtt
     5221 tcagatacgg gttgaaagta accaggaggt aaaagaaggg acactgttag ctattataga
     5281 tttgaagaag ttggagcaag cacaaaaaga tacggtgatc attgtagttt ctccaggcat
     5341 ttttgaagga aatttattaa aacaaaacca agaagtaaag attaaagata gttgttttga
     5401 attttagtgt acgatgaaaa aagttaaata gggtgatatt tatgggtgaa tttacaaaat
     5461 ttttttgaaa tgataagttt caatcactag atttaatcat tttgattttt gttaattatc
     5521 gaat
//
LOCUS       DAMEET010000120         5488 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.124, whole genome shotgun sequence.
ACCESSION   DAMEET010000120 DAMEET010000000
VERSION     DAMEET010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.124"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(60..278)
                     /locus_tag="PB780_002397"
                     /pseudo
     CDS             complement(60..278)
                     /locus_tag="PB780_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(544..738)
                     /locus_tag="PB780_002398"
     CDS             complement(544..738)
                     /locus_tag="PB780_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734418.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(838..924)
                     /locus_tag="PB780_002399"
                     /pseudo
     CDS             complement(838..924)
                     /locus_tag="PB780_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2090..3130
                     /locus_tag="PB780_002400"
     CDS             2090..3130
                     /locus_tag="PB780_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDE0734419.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            3954..4286
                     /locus_tag="PB780_002401"
     CDS             3954..4286
                     /locus_tag="PB780_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HDE0734420.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            4905..5246
                     /locus_tag="PB780_002402"
     CDS             4905..5246
                     /locus_tag="PB780_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HDE0734421.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1989 a          745 c          928 g         1826 t
ORIGIN      
        1 ggtattttta ttatatatag atttaacata tgtcggtcgg ttgattgata tagctaaagt
       61 tataactcaa ccgaagccga ttcatctaca cgaataaagt tgcgagtgtt ctcggctaat
      121 tcataaaaat tgttatgtgt tccatatagc aatactggaa taactttatt ttcttgaatc
      181 tcataagcaa ctctatacac ggtgaccgca tacttaaatc cattagacca attacctgat
      241 aaacctccag ttttttgctc ttacttaaat ggatcaatgg caatttcatc acaaacctat
      301 agaggatgag tgacttctgc ctaattacgt taaaaaatat tgaaagtaac tctatgtagc
      361 aaaaaatatc tagaatttca tttcctcaaa actgatcgat attgtatcta aaaatatata
      421 ctacctaaaa taggtagtat atattaaaat atgtgctttt gttagaaaaa tcaatacata
      481 aattatcata tctgtatatg tcatttaggt atttcagtta tgttataata gttttgctgt
      541 ctgctacccc ggcctaatct ggcaagggga ggaggagata taatgaaata cctttttgat
      601 tttgtttttg caatcaagcc acttgcagat gtagtgtgca gttacctctg caaatggctt
      661 gattgcaaaa ttgccaaaaa agacagtaag cctacccgtt aattctataa tatagaaacg
      721 taaaaaaaac cccatcagcc tgcacgctga tggggtttgg tgatataatg aaatacctct
      781 ttgttactca taattatacc ataattagtc acgaatgcaa ccaatagaaa aaactactta
      841 gaacggtaga tcatcgctaa taacaatgca ttgtccacca aatggatttt gtgaagcatt
      901 aaatacattg ttattaattg tcgctgcttt taactaggcc tctccccttc tattataaaa
      961 gaagatatat atcatttctt aattacctac taccttaaac caaaatttaa gtactaccta
     1021 atttaagtag tacttaaaca atctaaaaaa tagttatccc aattgttatg cttttataat
     1081 acgattattt gtaacaaaat gaatattata taattaaaca ctaccatatt taggtagtgt
     1141 ttaattatta gcaaaacaaa aaatatttat tctattctct taacttcaac taagttatgc
     1201 tgcacacctt tatataaatc atatgcaact cattagctag acaaactaca agcatgtagt
     1261 tttatctgtt aattatgtac tacctaaata aagtagttta taattagcaa gtatatatat
     1321 ttaaaaattt aagattaatt tttttatgac ttgtacaaat ttatctgata ttctcttatt
     1381 aagaactacc taatttgggt agttcttatt ttttacattt taagtagtat ttaattgcaa
     1441 tttggtagta tttttttatt atcttattct tagaacggta gtacttaata taaatatagg
     1501 tagtatctaa tttaagtatt aatggtagta tctaaattaa caggtagtat gtaactactt
     1561 taaagtaatt tttttcagat taacacagta gtatcttatt aaataagtta attttaatgg
     1621 tagtacataa ttattatata tatctgacat tggtagtact tattgtattt tttagtagta
     1681 tataatctat tgttttctag cctaaagcct tgtcttataa gaataatata atatatacta
     1741 ctgaaataag gtagtatata ttactagtaa tggtagtatg ttttatatgc ataaggtagt
     1801 tcttaattta ttttttgttt tgacactggt agtacttaat tatctttaat tttaaaacta
     1861 aattttattc atacgttttt ctggtagtat ttaattataa ataatattta tttaaagtag
     1921 ttcttaatga ctttaaaggt agtacataat tcattttatt tctgatttta ggtagtattt
     1981 aattaaaaaa cagttttaat gcgtttgcat tttttattta tctttgctat tttcgaaaag
     2041 agtaggtaat tttacataaa taaaacaacg tgaaagtaga ggatctacaa tgactgattt
     2101 taaatttttt aaggcggata gagtttacaa cgaattattt tatcaatttc caaaagtctt
     2161 tattgtttct gacgaataca aaaaaatgaa agattcaact aagattgcct atatgctttt
     2221 aaaagcaaga ttagagatcg caatcagcaa acggcaaatc gatgaagaag gtaatgttta
     2281 ttttacttat acgacaaatg aactatgtag agtattaaac tgccaaaaac aaaaagcgat
     2341 agcaatcaaa aaagagttgg aatcctttgg tttattatta caaaagcaga tgggatttaa
     2401 caaacagtta gggaaaaata atcctaatag actatatcta gcagaattaa aagtctcaga
     2461 aaatgatatc tacttactcg aaaaatttga tagagagaat agggaaaacg ttgataaatc
     2521 agagggtatg aaaatcatac ccaccctcga cgaaaaatca gacgctgaat cccttggggc
     2581 tcaagagggt atgaaaatca taccgtgcca aaacgttgat aaatcagagg gtatgaaaat
     2641 cataccagaa cttaataata atatattaga cactaataga cacaatatag acactgaaaa
     2701 agaccgccta caagatcaat tgttgttaga caattttgag acaattatga caaacgacag
     2761 cattgctacg tttgtccctg aacgagtatt aaatttgata aaaacatttt cttcaagtta
     2821 cagtgaagct caaaaaaccg tccagactat tcataatgca aagaaaaaag ctgaaataga
     2881 aagtgatatt tcgatagttt ttgaagaact cgatagttat tatgtcaatg cagaacaaga
     2941 attatacacg acactgttaa aagcctatca aaaattaaaa accgaaaaag tcgaaaatat
     3001 ccagaacctg atttttgtct atgtaaaaaa ttggtttatc gaaaaaccaa tagctgctaa
     3061 agtatcaagt gaaaaacgtt tgaattatgc aagctcccca agcactatta cgaaagactg
     3121 gttagagtga aactttactg acaaagtgat tgttgcttca tggagtaaca ttaaaattaa
     3181 aaatttttta ttattctaat tatattacga atattacaaa ataatagcta ttttaataga
     3241 atttataagt taaaatatta tgataaaaat cacaaacgta gccttttacg tcataagtgt
     3301 taaaagaaat aattaatttc aaatatagaa aagggaacgt atgtttgcat actccttttt
     3361 tttgagttat aataatacgc gaagagagat aatttcatta tttaaacaag ctaaaaatct
     3421 tgatattact tcttttcagg caatacgaat attattcgct tacatttttt gtataaattt
     3481 aattgtctgc tatgcttgtg atgaattgat tttcgttttg cacttttgga tgataaattt
     3541 ggacaattga aaatacaaaa aaaagaaagg cggtatgaac tcggaaggct ggtaaccttc
     3601 gtcagcgttc atacacggca ttaagatttg gcccttaatg cgttaacctt tcagtgttac
     3661 ggtattaccc gcaatggatt tagtataaca gatattcaaa agtgatacca ctttttttgg
     3721 ctgtgttaag gttaaaaata cgtgatatta cgatgaatct atgcctaagc ataatgttat
     3781 aaaaatgtcc gtgtggctct ttgccgtatg acgaacgctg aaattagagg gaacgcatgt
     3841 ttggatgcgt tccctctttt ttgtttgtca tgaacgaaaa tcaattctct atttttgaaa
     3901 aataacaaga aaaacttgtt aaaaatattt aaagaaattg ggtggtttat tttatgtcat
     3961 atacaggaat tttgagtctc aaagatattt gtcactacgg taaacggtgt accgctacag
     4021 agaaaatcac gaagaaacta tcaactggcc aaagtaaaac cgttgtgcag tgtaagaaat
     4081 atataatcca gaaagacaaa gtctcagagg aaatgattta ttacattggg aaacaaaagc
     4141 aaataatttt aaaagatcct atccccttaa aagagctata tccaacaatc aaacatgttt
     4201 atgaccaaaa cggtgtactg attggtcgaa gaaaaaatgg agtcttgcgt tgtaccgcaa
     4261 aagggatggg gcgtttgatc ggctgaattg atcattctca taacttgtat tttatcgttt
     4321 ttcgcttgct tgaaaatatt gtcgagactt ctaaaggaac ttagagtcac tagaaaaata
     4381 aggagtagac ttgatctatc aacaattccc ttcggggagt gacggggtag gttttaacga
     4441 ttggtaacgt aaaaaattgg tcaatcgagt aaatgcgctt gtttggatag tggtgaaatg
     4501 aatttgtgta aataataagc gcagagtaaa caagagggaa tttagaagtt agacaagtta
     4561 gaatagcgtc tagattaatc atgagcagct actaagggat ctagactttg acgcaaactc
     4621 atggaataag gagtaggtct tctcgtaatg gggaggacgc acaagaaatt gtgtcgtgtg
     4681 ctgcagtaca tgtagatact tgcgaggacg ttgattattg gatcaatcga gcatttaccg
     4741 ctgtctacta gggtttctcg tttcggacgc cataaacgag tcgaaatttt ttatgtattt
     4801 ttaagtaaac gaaaaacgat aaaatatgaa caagtcctga ataaagtgaa aaaattaata
     4861 taaagcatag agaaaatgaa atttacgtaa tggaggaatg acaaatgaaa aattatgatc
     4921 cgaatatacg attgggcaca catactatta aggtttcttt ccaacgttgg gattacaaag
     4981 gatttgtgac gtttagaagg ggcggaaatt gtaaaggatt agatgtttta gcattagacg
     5041 aggatgattt atacgatcaa acattgactg ataatccaat tggattcggc ttgctacctg
     5101 aagatgatga aggtaatgaa tggtttaaaa tgacgcttat gaatgataat ggagacgaat
     5161 tatctgttga agatacttgg tcatatttga gtgattatat tgtcagcttt gaaatcattg
     5221 aatttgtagc ggataaagaa gaataaaaac tggaggaata aattttacag ataaaggctt
     5281 agaaggagtc acgacgatta ataaaaaggt agtattacct taggcattga gtaaggaatt
     5341 tatttagtct aatggtgata agtcaagatg aaatttaaga ttttctttat tgagtaatat
     5401 gttttgaagc gcacgaaact agacccgatc aaaatcaaat ttttttcggg ttgattcaag
     5461 gatttgcgtc aataacggat tgttattt
//
LOCUS       DAMEET010000121         5466 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.125, whole genome shotgun sequence.
ACCESSION   DAMEET010000121 DAMEET010000000
VERSION     DAMEET010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.125"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            118..1107
                     /locus_tag="PB780_002403"
     CDS             118..1107
                     /locus_tag="PB780_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDE0734422.1"
                     /translation="MRDKEMRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAG
                     QYLFDKELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGA
                     MTICLSNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWET
                     CLNEFDKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFME
                     MQWINSNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVL
                     DQADFDLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1089..1550
                     /locus_tag="PB780_002404"
     CDS             1089..1550
                     /locus_tag="PB780_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDE0734423.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1600..2064
                     /locus_tag="PB780_002405"
     CDS             1600..2064
                     /locus_tag="PB780_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDE0734424.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2078..2827
                     /locus_tag="PB780_002406"
     CDS             2078..2827
                     /locus_tag="PB780_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDE0734425.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2827..3657
                     /locus_tag="PB780_002407"
     CDS             2827..3657
                     /locus_tag="PB780_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDE0734426.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3657..4304
                     /locus_tag="PB780_002408"
     CDS             3657..4304
                     /locus_tag="PB780_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDE0734427.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4402..5178)
                     /locus_tag="PB780_002409"
     CDS             complement(4402..5178)
                     /locus_tag="PB780_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDE0734428.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5260..5403
                     /locus_tag="PB780_002410"
                     /pseudo
     CDS             5260..5403
                     /locus_tag="PB780_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
BASE COUNT         1872 a          739 c          973 g         1882 t
ORIGIN      
        1 attttattat ataaaattgt tataaaaaat tttttttgaa aaatagtatt gcaaaataaa
       61 gcaatttcat agataatagg tatcgtaaag ttatataact ttataatacc tattagagtg
      121 agggataaag aaatgagaca actacacaaa gaacagattg aaacagcaat ggcagcgtta
      181 aaggaaaaga caattgatca tgtatatttt acagcatgtg ggggttcaca agccgtttta
      241 atggctggtc aatatctatt tgataaagaa ttaactgtac cttgtcatgt ttatactgct
      301 aacgaattta actatgctat accaaaagga ttatctgaaa attctttagt tatcacttgt
      361 tcccattctg gcacaacacc agaaactgta gaagcaacaa aaaaagcaag agaatttgga
      421 gcaatgacta tttgtttatc taatgaagaa ggatctcctc tatgggaagc agctgaatat
      481 cctgttcact atgattgggg taaagatgtt gatgctagtg atttaaataa aggaattctt
      541 tatggtttat tatttaatat tttacttgtt ttagatggct ctaaaaaatg ggaaacttgc
      601 ttgaatgaat ttgataagtt aaatgatctt gttattgctg aaaagagcaa atttgaaata
      661 actgctaaag aatggggtaa aaaccaaaaa cgagataaaa ttatttacac aattggtagc
      721 ggtattaatt acggagaaat ttactccaca gcagcttgct ggttcatgga aatgcaatgg
      781 attaattcta atgctattca ttctggggaa tttttccatg gtccttttga aatcactgat
      841 tatgatgtac cattcatctt agtgaaaagt attggagata caagattttt agatcaacgg
      901 gtagaagact tcactaaaaa attcagtaat aaattaatgg ttttagacca agctgatttt
      961 gacttagaat ctatagatag tgtagcgaaa gaatatattg cagctgtact tagtggcgtt
     1021 gttattcgcc aaatggtaga agcgattgct tttgaaagag gccattcctt agatgttaga
     1081 cgctatatgt ggcaaatgaa atattaatta ataggaggtt gcaaaagatg gcaaatcaat
     1141 tatttcacat tgtgattaca acacattcta ccctttgtga gggttattta aaggcaacaa
     1201 aattaatttt aatgaatgac gccacaggta ttagtgctgt tccatttaaa gaaaatacta
     1261 gtccagatga ttttgaggag agtatgaaag aaataattag tcagcatcat ttgcaacctc
     1321 taattatttt aacggattta attggaggaa cacctaataa tgtttctatt aaacagttga
     1381 acaacaatag aattcaaatt attgctggga ttaatttacc tcttttactg gagttattaa
     1441 tggcccagtc agctggtaaa tctatagaag aaatagattt taaggaatta ctgaataact
     1501 ctcaaaaaag tatggttcat attaattcgc ttttaaaaga tgtcagttaa ggtggcaatt
     1561 tctattctat aaattttaaa aattgaagga ggaaatagaa tgattaaatt agtgcgtgta
     1621 gaccacagat tacttcatgg acaagtaata ttttcttgga ctaaacagta tgatattaac
     1681 catattattg tggctgataa caaagttcca ggagatccaa tgtctgtgat ggctttgaca
     1741 attgctaaac ctgctgattg caaattagac attgttaaaa ttagtgatgt taaagggatt
     1801 atggaaacat ccccaaatga taattacatg attttaatta aaggacctaa ggaagctttt
     1861 gaattagttc aaaatttacc agaaattaaa gaaattaatt ttggcggtgt gtctaagaaa
     1921 agcgagtcca aacaatatgg aaaggcagtc tttttaactc ctgaagaatt agaagccaca
     1981 aaaaaaattc ttgatcaaga agtgaaaata tttgtgcaac aagtcccatc ttcaagtatt
     2041 gaaaatgtag attttagtaa gtaagggggg aaaataaatg ttattaaaag ccattttatt
     2101 aggtttagtt ggtgtggtag ctgttattga ctccaggtta attggtcgac aaaatattgg
     2161 tagaccacta attttgagta cacttgccgg acttgtgcta ggtgacttgc atacagggat
     2221 tgttcttggg gcatctttag aattaatttc tatgggattc gtctcaattg gggcggctgg
     2281 acctcctaat atgcaacttg gtagtattat tgccactgct tttgcaattt taactaatag
     2341 tagtacagaa acagccttaa caatagctat cccagtggct gtagctggtg aatttatgag
     2401 tattatcatg cgtatgataa ttgctcaatt tggacatgct gcagataaag ccattgaaaa
     2461 taataaattt agaaaagctc aaattattca tatttattgg tcatttattt ttaatgcttt
     2521 tgtttacttt attccaatct ttttaacagt atattttggt gctgacgttg tggcagattt
     2581 agtagataaa attccagaaa tgattacaga cgccttgact gtctctggaa atatgctttc
     2641 cgcattaggg tttgcaatgc tattaagcac aatgttgagt aaaaaattat atccttactt
     2701 tatttttgga ttctttattg ttgcctattc aggtctttca ctaattggcg tgacaatttt
     2761 tgcagctctg attgcctttg taatggatca agttaaatat ggaaaaagag aagaggcgca
     2821 tggttaatgg aaaatagtac agaaaaaaaa cgtttaatca caaaaaaaga tcttcataag
     2881 atgtgtcttc gttcatggac aatcatgtgt tcgtggaatt atgaaagaca aatgcatatg
     2941 ggatttatgt atgggatggc gccaatttta aataaagttt ataaaaatga tgaagagaaa
     3001 aaaagagaag cttatactcg tcatatggca ttttttaact gtacgcctca aatgactcca
     3061 tttattatgg gactttgtgc ttctatggaa gaacaaaatg ctcaagcaga agaaggaaac
     3121 tttaatactg aatctatctc catgattaaa accagtttaa tgggtccttt tgctggaatt
     3181 ggtgattcat ttttccaagg tactttaaga attattacat ttgggattgg attgtctttt
     3241 gctaagcaag gtagtatttt aggaccgatt ttagctgttg gcctttttgc tataccttct
     3301 attttagctg ttttttacgg cggtaaatta ggatatgagt ctggcaataa ataccttact
     3361 aaattgtatc aagaaggtct tatggatcga gtaatgcaat ttgcttctat cattggtttg
     3421 gctgtagtag gtggaatggt ggctagcatg gtgccaattg tcaccccttt aaaattcact
     3481 acagggggaa cagaacttgt tttacaagac ttacttgatt ccattattcc tggattatta
     3541 ccacttgtat gtactttggg aatatataaa ttgattaaga aaaatgttag tacaaatatg
     3601 ctcttattag gttgtatttt ggcagggttt gttttaagta taataggtat cttataatga
     3661 ttaaggctct aatttttgat atggacggag ttttaattga tagcgagatt atgtatcaaa
     3721 agtgggtatt taatttttta aaacaagaaa atttaagcta tcctttgtat tcttatcaaa
     3781 aaaagattgg aactacagca agtatttttg atgatataga aaagtacaat gagggatgta
     3841 acagtttaga attgaaggga aaatttacta aatattggaa atcgcaaaag attgattact
     3901 tggatatttt tcctaatata ctaattgaac agcttgaatg gttaaaaaag aaaaaaataa
     3961 aaatggcaat tgcttcttcc agtccaatgg atactataaa tgaagttgtt accacttgta
     4021 aaatagagtc atattttgat tgtttaatta gcggaagaga tttgccagaa agtaaaccta
     4081 atcctactat attccttaaa gcttcagaac agctcagaat tccagttgaa gaatgtctgg
     4141 tgatagaaga ttcttataat ggtataaaag ctggtaaaag agcaaatatg aaggttcttg
     4201 caattaaaga taaacgtttt tctcaagatg taagtctagc agatgatgtt atttacgata
     4261 ttaaatatct gcgacaagaa gtacaggtga aatttggaat ataaataagt tttttgatgt
     4321 atttagagaa tattagtaaa aataaaaatc atgcccaaat ctaatcaaaa ttgattgctt
     4381 tgggtatgat ttcttttaat atcaatctat tactttagga tctgaatcat ttttatgcac
     4441 cgtatctaaa gtgatatttt ctgtgacttc aataacataa ctaattctat cacaacgaac
     4501 tgtggattga gaataatgaa atggttgatt atttttatct ttgccaacct tttctaaaat
     4561 aaatagcgga tctcctacta tacattgcaa taagtcggaa agctcaggtg ttgcagataa
     4621 accgtttatt tccataatag agctcgtagc tttgacatca tagtcatctt ctagaagttt
     4681 atataatgaa gtatattggc tacctttttc aaataaagtt ggaaattttt cttctgatgc
     4741 gaaaaaatta tcaattccca tcggggcacc gtcttcccac attaaacgtt taatattgta
     4801 aactctatca gttacattga tttgtaattt attagctaaa aactcatcag cctttataat
     4861 tttagaatgt aaaacagtat atctaatttt atgtcctcgg gtagaaagag aatcgctaaa
     4921 tccatgaact ccagctaatt ttaaactttc ttttttttga gttacaaagg tccctttccc
     4981 ccttaatcgt tcaactgttc cactttctgt taaaagttta aaagcttttc gaatggtaat
     5041 cttacttact ccataaattt tacataattc ttcttctgta ggtaattgct ctcctggaag
     5101 atattttttt tcacgaatat cgttacgtat agaagcttct acttgtagat ataatggtct
     5161 attatcttga aaattcatag tacactccct aaaaagttat acttcattat atcacaattt
     5221 attaaaataa aagattaaga tataaatata aatgaactag tctttgacta accaatgccc
     5281 gcttttgaaa gaaaacaaat tcattctgtt ttaagtaaaa atccttatgt taccactcat
     5341 tctgaagggg atgagcctta tcgctacttg gttgtggaac caagcgataa gacaatttta
     5401 taaattcaat attaattaaa ataaaaacaa acaaagtaga ttattttact ttgtttgttt
     5461 ttttat
//
LOCUS       DAMEET010000122         5298 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.126, whole genome shotgun sequence.
ACCESSION   DAMEET010000122 DAMEET010000000
VERSION     DAMEET010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.126"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..674
                     /locus_tag="PB780_002411"
     CDS             <1..674
                     /locus_tag="PB780_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HDE0734429.1"
                     /translation="SAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDT
                     LRKAFFYAQEAKISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLE
                     DPSLSYLKLALTDKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLA
                     DYLGIESSDIMAFGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYI
                     LKNFY"
     gene            714..1211
                     /locus_tag="PB780_002412"
     CDS             714..1211
                     /locus_tag="PB780_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDE0734430.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(1596..1669)
                     /locus_tag="PB780_002413"
     tRNA            complement(1596..1669)
                     /locus_tag="PB780_002413"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1633..1635),aa:Ile,seq:cat)
     gene            1856..2179
                     /locus_tag="PB780_002414"
     CDS             1856..2179
                     /locus_tag="PB780_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734431.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2343..2597
                     /locus_tag="PB780_002415"
     CDS             2343..2597
                     /locus_tag="PB780_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734432.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            2667..2912
                     /locus_tag="PB780_002416"
     CDS             2667..2912
                     /locus_tag="PB780_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDE0734433.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            2988..3353
                     /locus_tag="PB780_002417"
     CDS             2988..3353
                     /locus_tag="PB780_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734434.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(3414..3977)
                     /locus_tag="PB780_002418"
     CDS             complement(3414..3977)
                     /locus_tag="PB780_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734435.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(4545..4745)
                     /locus_tag="PB780_002419"
     CDS             complement(4545..4745)
                     /locus_tag="PB780_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDE0734436.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            5141..5290
                     /locus_tag="PB780_002420"
     CDS             5141..5290
                     /locus_tag="PB780_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734437.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
BASE COUNT         1668 a          934 c          871 g         1825 t
ORIGIN      
        1 tttctgctaa agatattttt tccgaattag gaatcggtgg ctatttgatt tcttccaatg
       61 gttcactgat tgctgaaatg aaggatggaa aaatggcgaa agttctcaga cgttcaaaaa
      121 tagatagcga tacactgaga aaagccttct tctatgctca agaagcgaaa atatccatca
      181 ttgctagccg agaaacccaa gatgatcagc tgacattcga tgaatcaaat ctagtcaaag
      241 atgaccctta ttatgaacat ttcaatttac agaaccattc ttttgatcaa attttaaata
      301 tgttggaaga tccttctctt agctatctta aattagcatt aacagataag agtgaagaaa
      361 aactacagaa gctacaaaga gatctgcaag cagatggtat ccaatcagta ttctctgatc
      421 cgcatttctt agaaatcacg cctacaggta tcactaaagc tcattcactt gtttttctgg
      481 cggattacct tggaatcgaa tcgagtgaca tcatggcttt tggcgatcaa gaaaacgaca
      541 ttgctatgtt gaattacagc gggttgagta ttgcgatggg gaatgcacag cgacaagtaa
      601 aagaaattgc aaaccaagta acactgacga atgaagaagc tggtgttgct tatattttaa
      661 aaaacttcta ttaaaaagaa taaaatgacg atatgaatgt aggaggaaag aaaatgttag
      721 ttattattga tatgcaaaat catattcttg atccaaacag cgaattttat attccagagt
      781 cagatcaatt ggcagaacgt attgctcaaa gattagctaa agcacgccaa aataatgaat
      841 atattctctt cacacgggat ataccagttg aactgaagga caaagaagaa gaacgagaag
      901 atttgcaatt gatccctctt ctttctccac aagaaaatga acgagtgatt aaaaagtatt
      961 actttacgat tccacctgaa actttagctg aaatccagga aactttattt gaaagcaaag
     1021 aagaacaaaa agagatcgaa gtagtcggga ttgaaacaaa cttgtgtgta ctctctaatc
     1081 tgataggcct gcagagtgct tttcctgaag cagacttttt tgttgatcca atgttagtct
     1141 ccagtagaaa acacggagaa tccgctttag agctgttgaa agatttcaac gtctctatcg
     1201 tcgaatcata aaaatatcta tcttcattta cgatatttaa cacacaataa aaaaagcacg
     1261 cagaactgtt ttagtcctac gtgctttttc tatcaaagta gattggcttt tttatgacac
     1321 agagtgaaac ggcaggctca cgacctcttt cttaaatggt gttcaatcag ctgaccctcg
     1381 gtattagctt aacaaacggc aaaatatgag gaactatttt tccgtttgtt aagctaatac
     1441 tcaggtcaaa acgctgattt cacaacctct tccttaaacg gtattcaaaa gcttgcatct
     1501 gcagtaataa gaacagccaa gtaaaaacat aagaagctgt ttttacttgg ctgttcttat
     1561 tacttgatgc agggcagctt tttcacaacc tcttatgtac cttgtaggac tcgaacctac
     1621 gaccggacgg ttatgagccg tctgctctaa ccaactgagc taaaggtaca tcataaataa
     1681 aatttatttt tgttactttt caactatagc acgaatgaca aactctcaca agtttcaaaa
     1741 tataagtaaa ataatattgt tattcttatt tcttttactt gtgtcataac tattttgtta
     1801 ctttaatttt tatttgctta ttttagaaaa aatgttattc ttttatttat tctcaatgtt
     1861 aggagtgatt attttgccat ttattgcgat tttattcgat ttagttgcgg cagcagctta
     1921 ttttctccaa ttaaattatc aaacctcagg aattctgttt attggaatga tttttcaagg
     1981 tgtgattacc cttttgcttc ttgtcatgat gctcagctat aaaggaaaaa gatatgcccg
     2041 tattcaaaca aaagtatttg ttaaatatgt ttccattcgc tatgggatca tcattctttc
     2101 ctttttagtg aatgcgatcg tcctcttttt atatgtattg aattacttag atatcaatcc
     2161 attgattttt tctcgataga taagacacat tcagcggaac agttcctgct tatcagcaga
     2221 aaactgttct gctttttcat ttttcattga tctattcttc ttttattttc ctagcatttc
     2281 atttgtgatg tatgcaataa catgttatct tttattatat gaatcgttga ggagtccgtt
     2341 acatgattac gttggtgaga gttttgtttt gggtgcctgc ggtcgcactg gtcgcaagta
     2401 ttgtttatct tatgaattgg aacaaagaac ggttttattt agctattttg actcttccgg
     2461 ctatctactt tatgtggaaa gtttttaatt acaactactt tgaacccgat agtgttttta
     2521 tagaagaatt atcaggactg gttttgtctt tattgatcgt cattctttat ttgatacgtt
     2581 taaataaaaa acattgagta ctattgattg catgtgtagc tttctatagt cattataact
     2641 attctactat aatgaaaggt gattaaatga gtatcgaaga agctatttta ctggatactt
     2701 tacgttgtcg tctgaatttc ttaagaactg gccgatcttt atctcaagta gctgtttcta
     2761 gcgcgttaag tattccttta ttcaaatatc gtaagtacga agaaggagaa gaaattccaa
     2821 gcatcattga attaatctat atcgctgact tttacgatat ttccgttgat tacctaattg
     2881 gtcgctcaga aacagcaagc aaactgaagt gattttttcc agccttggtg ctggtttttt
     2941 catttaacgt ctaaggttaa caatctatat ataaaggaga catttctatg cgattatggc
     3001 atgaaaaatt gatttcaaag cttcctcgtc aacagctttt aggacaacat cgggaatgct
     3061 gtgcattaag aggaaatggc tggggaaaga aacatgctac tgtggattat gtttttcact
     3121 attctcctta tagattattc caatatcatc agcttgtcat ggatgaaatg gaaaaacgtg
     3181 gttacagccc agcaccagag tggaaaaatc ctatgtatcg tggcaaatca tgcgaagcat
     3241 tttttgattt acctactgta gagcgcaacg tgccgattta tccggaacac gatgaagttt
     3301 atctagcaga atgtttagca aatttggagc aaaaaggaat ttatcttacc taatataact
     3361 agaaaagcct tctatctcct cttacttcat taaaaagctt taaccatctt agatcaagct
     3421 aaaatgtcgt aagttttagg gttatcttca tcctcacgtg caaaactaaa agcgtactct
     3481 agttttggtt catcgccaac ttccctagac ataaatgaac atctcctgac gtggtttaaa
     3541 agtaccgcat cttcgacgtc tgtatacgat gcacagcaca gattgcttct tttgatcttt
     3601 cttttgattt ttctgttgct tcttctacac cttcttcgat tactacatcg atgcttacaa
     3661 ttgcttctgc ctaatattcc catgcatcgt cgatttaagc ctctgtagtc actgtgatcg
     3721 tttccacatt ttaactcctc tgtcgtggct tcagttgatt ttgtgatcag ttcagttgct
     3781 aagccagagt cagaacaacc tgccattgct agcgttaata ttgatagtaa aaatagtttt
     3841 tttatcataa ggttcccccc ctgaaaatag actaaaataa taacgctttc tttttttgtt
     3901 ttcagacgaa aattaataat ttctaaatat tctttaaaaa agcttataac tattatgcct
     3961 tttttaatat ataacacagt tatttttaaa aataagaatg ctaaatcact ctacaataac
     4021 ggcgaaagca caaaaaataa aaaaattatt acgttattaa aaaaacagat gaaagatcag
     4081 gttttattag atctcattct ttcatcactt gaagggattt aatgaactac tttttcctct
     4141 ttgtcgtttt tatatattcg tattccttca aacagtagga tagcctcttt caatgcttga
     4201 tatttgaact gtccgttact taaaaattta gatgtgccgc cctcttccta gaacatattc
     4261 ttttttactt tttcattaat tgacagacca atcgaacgaa actgtaaatt tcgataggat
     4321 aatgattggt caaactcatt cattttcaag acatctcgat tcatattcaa gataggaatc
     4381 tttttatcaa catcacttta ttcgtatttt cctctaccat aaaagcttca cgctccacaa
     4441 aattgtttta tacactacta ttcagcccat tcaacgacca atgaattttt tcgtacaaaa
     4501 aaaccgtctt ttctttcgaa aagacggtta aaagtcaatt tttattaagc tttaacaatg
     4561 tttgttgctt gagggccacg ttgaccatct tcaacatcgt aagacactgc ttggccttct
     4621 tctaaagttt tgaagccgtc accttggata gctgaaaaat gtgcaaatac atcatttcca
     4681 tcttcaccag tgataaagcc aaaacctttg tctgcgttga accattttac tgtaccgtta
     4741 ttcataaata aattcctcct gatacgtatc aatgtacgtt ttttcgttgc aataaatatt
     4801 tgataggcaa aaggagtttt atctatgtag atatatcgct caaattacgt ttcaaaatag
     4861 attactcatt taatatatca tgctttttct aataacacaa ggataatgca atgaaatata
     4921 aaattcattg tcttctattg tcttcgaatc gaagaaaaag ttgaataacc gcgattgatt
     4981 cactttttgt tttcttgttt atttgctttt atctgcaact tttgacttta aaatttctta
     5041 actatgaaaa cctttcaatt ggtggtatac ttataaatgg aatttcgtta tcaacgcgtt
     5101 cgttgccttt cagcaagtat ttaaagaaag gagaatggct atgacattct ataacaaaga
     5161 tatacaatat cgagtaacct tggatacaga tttgaatcta tttgtcgttt ttgacaaaga
     5221 aaataccaat catgtagcga caggtgttac gatcgaacaa gcagtgcaag aattaaaaaa
     5281 atctgcataa ggagtgga
//
LOCUS       DAMEET010000123         5223 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.127, whole genome shotgun sequence.
ACCESSION   DAMEET010000123 DAMEET010000000
VERSION     DAMEET010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.127"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            274..870
                     /locus_tag="PB780_002421"
     CDS             274..870
                     /locus_tag="PB780_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDE0734438.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(928..1410)
                     /locus_tag="PB780_002422"
     CDS             complement(928..1410)
                     /locus_tag="PB780_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734439.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1516..1713)
                     /locus_tag="PB780_002423"
     CDS             complement(1516..1713)
                     /locus_tag="PB780_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734440.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            2008..2766
                     /locus_tag="PB780_002424"
     CDS             2008..2766
                     /locus_tag="PB780_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDE0734441.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            2826..3254
                     /locus_tag="PB780_002425"
     CDS             2826..3254
                     /locus_tag="PB780_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734442.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            3300..3372
                     /locus_tag="PB780_002426"
     tRNA            3300..3372
                     /locus_tag="PB780_002426"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3333..3335,aa:Gln,seq:ctg)
     gene            complement(3522..3893)
                     /locus_tag="PB780_002427"
     CDS             complement(3522..3893)
                     /locus_tag="PB780_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDE0734443.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            4108..4293
                     /locus_tag="PB780_002428"
     CDS             4108..4293
                     /locus_tag="PB780_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734444.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(4376..4564)
                     /locus_tag="PB780_002429"
     CDS             complement(4376..4564)
                     /locus_tag="PB780_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734445.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(4588..4851)
                     /locus_tag="PB780_002430"
     CDS             complement(4588..4851)
                     /locus_tag="PB780_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734446.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            5009..>5223
                     /locus_tag="PB780_002431"
     CDS             5009..>5223
                     /locus_tag="PB780_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HDE0734447.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVI"
BASE COUNT         1722 a          995 c          808 g         1698 t
ORIGIN      
        1 tttttaatgg caatatcata aaaaaaagaa gatagagatt taataattat atatcagctg
       61 tttttttcaa ataagcaaaa attcctaaaa cacaacaatg aaagcgataa ctaagaaaat
      121 ccttacataa cgatttttct tattaaaatt gttttttgaa cttcgcttat tttttgtgag
      181 catatctgtt tacttacttt ttgtaaggtg ctatacttca tttcgtaatc aattaaaaaa
      241 atgcaaaacg caaatcaaag gagaaatttt attatgacaa cttttactga cactttgaaa
      301 aaccgccgtt caatctatca tctaggacgt aatgtttcac tttctaatga agaattaaca
      361 actttgatca aagaagcaat taaagaaagc ccaactgctt tcaatgctca atcaacacgc
      421 gcagttatcc ttttcggaga tgcacatgaa aaactttggg aaatcactga agaagcactt
      481 cgcccattga ctccagcaga agctttccca aatacacaaa acaaattagc tggtttcaaa
      541 aatggttatg gtacagtatt attcttcaaa gatactgacg tggtaaaagg tctacaagaa
      601 caatttgaat tatatgcaga caacttccca gattggtcag aacaatcaaa cggaatcgca
      661 acagctaata cttgggtagc attagttgat aaaggattgg gcgcaaactt gcaacattac
      721 aacccagtta ttgacgaagc agttgcaaaa gaatggaata tcccttcaaa ctggaaacta
      781 cgttcgcaat tagtattcgg ttcaccagaa acaccggctg gggaaaaaga atacatgaac
      841 gatgccgatc gcttccgcgt atttggataa ttttatttga aataaaaaac agaggttgtg
      901 acaaacatct tgtcgcaacc tctgttttta ttctggatca atcataatat caatctctgc
      961 ctcatcgttt aagcgttggg acaaaaatgg aactaattct tctaatgatg cagaaaattt
     1021 cagcatcggt cctcgccatc cgtttcggaa aaaattcatt tttacgattt tagaaccttt
     1081 tggctgatat aatacaattt tattgatctc agaaaaagga acaccttttt tgtcaaagct
     1141 attcaaaaca agtccttctt ctgtgattcc tctactgtcc aacaaaaaac taataaaaat
     1201 cattgctgac aagatccctt ttgccaattc atctgctgta ggatgactga tgagtgtata
     1261 ccaaatgact agtgacacta agaagggtat caccaagtag acccatcgat ttcgcacagt
     1321 ccgataaata atttcttttc gaaccagcca aagaaataaa aacatcacaa caacgaataa
     1381 aaacaataga accgtaaaga atgttaccat gacaactcgc ctcgcttttt cttctccaga
     1441 aaataggaaa agaatactga tttaccttct caaagaaatt ggacttgcag cacgccgcac
     1501 gtccaattgt tttctttatt caaatgtatc atattttgca acaaccatgc ttgctttttc
     1561 atctaataat tcttggtcct ctgcagcaca ttgggcaact ttgaccacgg cgcagtttgt
     1621 catttttgat acaacttcac caatgaccgt ttttgtaaaa ccaattggtt tgcatgcata
     1681 ttgttctcct aaattaatca tttcttccgt catcaaaatc ctcctaaact gctggaactt
     1741 ttctccacat taccactgct atttctttgg agatttaact cattgatttt tatttacagt
     1801 taaaaatcaa ccttacaggc attatagcgt actgttttcg ttttcacaat taaaacagct
     1861 gttgtattta gaagaatttt tttcaaaaaa ttaaaacata ttccttaaaa actaattatt
     1921 tcctacatat taaaatataa tacttccatt tccaataaaa atacatttat aataggaaag
     1981 ataaacagct gaaaagggag atttcaaatg aagaaaaaaa cgttcaaaaa actattgttt
     2041 ctagggggca cagctgctct tttattaagc ggtacaagtt attacctaca tatccattct
     2101 ccagaacata caatgaacac tgcacaaaaa agttcaacac aaaaaacgaa aaagacaaaa
     2161 gagaaagcag aagttttact agatgttcca ttagagagcc agtttgattc tccttcttta
     2221 gaaaatggct gtgaagtcac ttcgctttct atgcttttat ctttttatgg ttatgaaaca
     2281 actaaaaacc agttagctga gcaattggat tacgtccctg ttttcaatgc agatgggact
     2341 catggcgatc ctaatgaagg atttgttggt gatatcagtg gcggagactg ggctatggtc
     2401 gtctatgttc ccccagttgc ctcccttgct cagcaaatcg tccaaacgga ttatcacact
     2461 tttccaaaaa ccgatgctca actcgatgat atcaaaaaag cactatctaa aggaaatcct
     2521 gtctggactt ctgtgactat caattttgaa gtcccagatg aaactgattt tatgacttgg
     2581 accacaaata atggagaagt aagagtcact cctcttgttc atgcttgtgt agtcactgga
     2641 tatgacaaag aaaatattta tgtcaacgat ccctatggtg taaaaaatcg agcagtaccg
     2701 atcaacgatt tttcggcgat tttcactgca atgggtggac agatgctaac tttggagaaa
     2761 agctagtaat tcaaacaaaa atcccttata ctcaagatac caaacctttg aagggagcgt
     2821 agataatgga aaaattcaca cataaaaaaa tggatccaaa tgaaattccg attatttttg
     2881 tccgtgactg caagggaaat gttcaaggta aagtctctat taatgagtgg aacgagcgtc
     2941 gcagacctgc taccctcaat gaactggaaa tcaaattgta tcgtcaatct cttgtttatt
     3001 acgcagatca agagtacgag aaagccaccg atttattgaa atttctaatt gctcgaacag
     3061 agtatactcg ctttgaatat attgaacgtt tagcaaacat ctatcatatt atgaatgaac
     3121 ctgtcaaaga atatcagtta ttggacaccg tcttgtcagt agcagaactg attgcccttc
     3181 ctgctggatt agaaaagaaa ctagtacgcc gccttttacg agtaaagcaa caactatccg
     3241 atcaagaaaa ataacttgcg tttttttagg aaagtgctat gattattttg ttgaataaat
     3301 gggatgtagc caaactggta aggcacttga ctctgaatca agcaattgta ggttcgaacc
     3361 ctgccatccc aatttgatgt gcttgctgaa aaaatgaacc ccctgtaaaa gaacaatctt
     3421 tttgcagggg gattattgta ttatttttac tacttgtatt tagtttacat aattaaatta
     3481 tgaataataa aatcgggcgt attcttaata caaaaactga tttacccaat aattgctaat
     3541 gctttctccg gaactttttc tctaggaact tcctcatacg tttctatgtg tgctccttta
     3601 ttgctaattt tcaaataatg attttctttt aacacagaaa gaccattaaa ttcaaccgtt
     3661 cgttctttcc cattggcatc agcagctttc tgcacatacc ttgctgtacc atattcattt
     3721 acttcctctg gtttttgtgt ctttacatag acctctcctt tttcaactaa tggatttagc
     3781 tgatcaagta taccagggat ttctccataa cttccttcag tataatattt cagtccaaat
     3841 agcaaaatac cactaatcgc cgccagtcca atcaaatatt taatcacttt catctttcta
     3901 cctccttgtt atatattcag tatatgggtc agttaaagaa attccatccg tcccaagttg
     3961 gagatttttc tcctacttta gaagcgagaa tcgagtgttt ttttagtaaa aatgatcgtt
     4021 atttgatatt attgacgtgt ggataaatca gtttttctcg ttttgtccat gtaaaaaatc
     4081 atgttgatag caaaggatgt gttcaaaatg aatcaagaag aattatttca aaaagtcaag
     4141 gaaatgatta agaacggcaa tttcgatggc gcaaaacgat ttatcgaaga acataaagaa
     4201 caattaggac cttataaaga gaaagcccaa aatcttttaa aagatgtaaa tatcgacagt
     4261 gtcaaaaaca aatttaaaaa tctattcaaa taagtaaaac tttcactttt gtaaaatcaa
     4321 aggaggacaa tgctgctgca tcgtcctcct ttggttcttt tctgaatact tcatactaaa
     4381 ataaacgtgt cggatcgtaa tgataagtca cattgccgtt cagtgaggct tttcccacca
     4441 catatttacg attctccaac ttcagccacg cttctcgaaa agccattaat tcttctcttt
     4501 ttacatcaat cttttcgatt tttccatcga tcaaatcttg aataagttgt tcatatggat
     4561 tcattttttt cctccatctg ctgtcaatca ataagttgta aaaataggtt catatgcttt
     4621 tttggtatct agatcttctc tgactttgac tgtcgtataa ataatttttt ctactgggat
     4681 actataataa cttccttcac cattattgaa tcggtgaacg tctttctttg ctaaaatacc
     4741 atttatttta ttcgtcagta cacgttgttc gacactgtta cttacaacat agtaaatcac
     4801 ccgaatatta ttgatcaaat gcaaatcgat ttctaacact acatgatcca tgaaaatccc
     4861 actccttctt tcactttgat tatagcaagt ttctcctcta atttcaaaat cgatacagat
     4921 tttaccggat aaatccacaa aactgcttgc gccatttcgc acaaagtgtt attttgtatc
     4981 atggacttta tttatcggag gcaatagtat gatcgcgatc ggtcaatttg ttttttatat
     5041 tccttttttt attatgataa gcattctttt ctattacata aaatggacaa aaaagaagtt
     5101 ttcagtttta ctggcctctt tacctgctgt atattttact tatcaaattt tttcttttag
     5161 gcattgggaa acaacgtctg tattaataac acacatcatc gaattgacgc tttcagtaat
     5221 ttt
//
LOCUS       DAMEET010000124         4951 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.128, whole genome shotgun sequence.
ACCESSION   DAMEET010000124 DAMEET010000000
VERSION     DAMEET010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.128"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1029)
                     /locus_tag="PB780_002432"
     CDS             complement(<1..1029)
                     /locus_tag="PB780_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734448.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLY"
     gene            complement(1237..2163)
                     /gene="glsA"
                     /locus_tag="PB780_002433"
     CDS             complement(1237..2163)
                     /gene="glsA"
                     /locus_tag="PB780_002433"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDE0734449.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(2186..3613)
                     /locus_tag="PB780_002434"
     CDS             complement(2186..3613)
                     /locus_tag="PB780_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDE0734450.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(3644..4858)
                     /locus_tag="PB780_002435"
     CDS             complement(3644..4858)
                     /locus_tag="PB780_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HDE0734451.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1551 a         1042 c          852 g         1506 t
ORIGIN      
        1 gtacagaagt tgttcttttt ctgatagaag atagatcctt tccggtaaaa atacttgtcc
       61 atctgcccac aacgtacgga caaattgtaa aaaggattgc ttgaaaataa aattttctgc
      121 atcgtcgatt tcagcgatac taataatctg taaaatcaat tcattcagtg ataggtcatt
      181 gagaacaaaa tttttataaa caacttcgaa atcttttctt aacagctcca tgttctcatt
      241 ggtataattt ctactgttga acaaagaaaa aatataatag acatcttctt cttgtaagtc
      301 tagaattcct ttttgctctt ccaatccttg acagactaat tgaaataacg gcttcgtttc
      361 tgcgcttttc ttatcttctt ctttcagtcc aaggggaaat gcctttcttc cgattgcgat
      421 ctctattcct cgtatgataa aggacttttc atcttccgaa tatcttcttt taaaaaaatg
      481 agtctctaca taatcaatca actcgttaca agcttcttct acatacggat ttttattttc
      541 atagccttcc caattgacat aacgtactaa agctagcaag atgttccgcg agtcaaattc
      601 tgggataatg atttctttat ttttcaaata atcattttct ttaaaaaaat ccaatacgat
      661 ttttttgatt gtatatactt tgctcaaaga aataaattca agatcagcca gttctgtact
      721 cgtgaatata ccattaaaat aataacttaa aaactttaaa aaataagatt gaccgtacag
      781 aaactgtgtg agctcagaaa gagcaatacc atgtttgaat acgagtttat acttatgctt
      841 ctctcttact tgttctttta tacaatattc tagacactcg gctagacttt ctagatcgtg
      901 aaggatcgtt ggtgtagtca cccttagaga aatagctgtt tgttcgatgt ctatttcttg
      961 gaattcaaat aattgttcac acagatacac ttttctaaat atatttcgct cgatgtaatt
     1021 ttccagcatt cctgatacac gcctccagtt tttcttatga ttttatttaa tcatatctgt
     1081 aaatcgtatt tttgtttcct ttttatttag aaaagccctt tcaaaaacca ataaaaaaag
     1141 aaactgggac aaaagtcaaa caagctcaca atttcagcct cattgtaggt agctttgagc
     1201 cgtttggact tttaccccag cctctttcaa gaatggttat tcaaagatgt cagcgtctag
     1261 ttctttgact acatctttca acaattggat accagctgcg ctgttaccaa atggatctag
     1321 tgccgggctg aagacaccta tgccgtaacg atttggcaca gctgccatca acccgccgcc
     1381 tacaccgctt tttgcaggta caccaacatg gacggaaaac tctcctgatt catcatacaa
     1441 accagcagtt gtcataattg atttgacgat tgtagcactt tcttcagtaa tgatttgttt
     1501 gccattccaa ggagcaattc ctttatttgc cagcacagca gctaattttg ctatgcctgt
     1561 agcggtgaca ttgagggagc attgtctgaa ataagcatcg agtatctccg gtacgtctcc
     1621 ttcgatcatt tgattacctt tcatatagta agcaagagag cgattgatat cacctgttct
     1681 tgattcagaa tgatagattt cttcattcaa tgtgatatta ggatcatcac agatctcttt
     1741 cataaatgac aagattcgct caaatttttc atctgcatct ttaccggcaa tcaacgaggt
     1801 tgttgcaatc gctcctgcat taacaaaagg attcattgga tgtttgcgat gattgatttc
     1861 catattcatg atagaattga aggcaaaacc ggttggttcc gtgttgattg tttgaaagac
     1921 tttttctatt ccgttgtctt tgattgctaa taacaatacg ggaacttttg acatgctttc
     1981 gatggcaaag cgcacatcgg cgtctcctcc ctcaatcagt tgttttgtat ccaaatcata
     2041 aatcgccata cctaattgtt tgggatccac ttcagctaat gctggaatgt aatccgccac
     2101 ttgtcctgtt tgataatagt ggaaattttt tccaatcaat tgttctaatt cttttacttc
     2161 catttgaata accagctttc attttttatt tttctgcatg ctggaattta tgacggcatg
     2221 cgtagatcgt aaatggaata actaagacga taagataaga aacaatcaga atcgtttcat
     2281 aaatcccatg ctgtccagct gcgatactag caggtggaac aaatgagata caaaaagcaa
     2341 aaagtgaagt caaaagacct aaacaagcaa cgattacttt tacaactttt ccgcctggaa
     2401 cctgataggc tctttcaaga ctgtcatctt taaaaaccag cttcaaatag cccgcgaaaa
     2461 acataaagta caacatcagg tagattacaa cagtcaatga catcgctgca agaaatgaaa
     2521 gattattccc cccgccgctt aatgtaaaga cggctgccca gattgttgca acgatcccat
     2581 ttgcgatcat caaatgagca ggaacaccgt gtgtgttttc ttttgcaaaa tattcaggaa
     2641 gtaatccttc ttctgctgct acgtgaagtg cttttgaagg agaggttacc caagaagcaa
     2701 tttctccgat gacacctaaa caaacaagaa tcgcaagaat tttcgcaatc catgcaagat
     2761 ttggatcaat gtgtcttaat aggaattcta acgattgaat cacccctgta ttaagagaca
     2821 aaccatgttg aggtactgta gtagcgacgg tcaatccgcc aaaagtatct aaaatgattg
     2881 ccagaataac caatacaaac attgctaatg gataatcctt tttagggttt tccatttcat
     2941 ttgcatgact ggcagatgtt tctacgccca tatatgctaa aataaaggaa acaaaaacaa
     3001 ccagcgtagg caatttcgaa aaatcaggaa tcaatgtttt catagacagt atcgtttgta
     3061 gtggagcgcc tgaagcgaca taatggatcc ctaagccaaa caagactaat gctggcaaca
     3121 agataccagc tacaaaaccg atccgagcaa tcaatgaagt gtatttggtt ccttttaact
     3181 gtgagagtaa aatagcccag aaaataatca ataggattgc caatttcaga tatttattgt
     3241 tttgtatagc accaataccc gttgcatagg ataatgcacc agttaagaaa tacagcatgg
     3301 tcaagtagcc aactgttatc tcaaaccatt ggaaaaagat tgcggcaaaa ccgaatcgtt
     3361 ttcccaatgt gcgactgacc caagtataga caccgccttt ttcccatcct ttcaccgttg
     3421 ccatctctgc tgcgcacaat gccacaggga taaaccatag caaaccgcct aataacaaga
     3481 aaaatactaa gctgaagcct gaggtcgcga atgtgggata ttcatataat gaaacgacca
     3541 ttgagatggt catagccaaa aagccaaaga acgttaattt ttttgcagga attttcgttt
     3601 tgctttgttg catgaatctt cctcttttct atttgaattt gtttcaattg agtttgttgc
     3661 caaagaagaa aaagtccaat ccttcttctt gttcttcttt tgtagtagtg atcgttgtaa
     3721 agtgcatcgc cgattttaaa atcactgttg tcagcacata ggcatacacg gcagtaatga
     3781 tgacaccaat cacttgaatc cctaagaagt accagctgct tggattcgtt agtaaatggc
     3841 tgttagatgc aaagatccct atcagaatcg tacctgtgaa tccgcccatg ccatgaacac
     3901 cccacacatc aagtgcatca tcccattgtt tctttttacg aaaatcaaca gcagcatgac
     3961 agacgatacc cgcaatgatc ccaatcagca tggcactttt tgcttctaca taaccggcac
     4021 aaggcgtaat ggctgccaat ccagcaacgg atcctgttag tacgtcgaca aaatcaaagc
     4081 catttccttt tacttttgca atgatcagcc aagtaatcat cgcaaatgca agagcgatga
     4141 atgttgaacc aaatgcattg actgcttgtt catctgctcg aagtgcacca ccggaattaa
     4201 aaccgaacca gccaaaccaa agcagtcctg taccgatagc agctgccata agactgctat
     4261 gctgacttct ttctcctaaa ggaatctttc tttgaccaag tgtgaggata catgctaaag
     4321 agccgaatcc agctgtcgtg tggatgactg ctccaccagc gaaatctcta aatcccatct
     4381 gatccaaaaa tccgccaccc catatccagt ggcaaacagg gaaataaatc agcagattcc
     4441 aaaaaataac aagtataata tagcctttta aattcaatct ccctgcaaaa gctcctgtca
     4501 tcaaaggaac ggtaatcacg ataaacatca gttggtaaag aaagaacatt aaaaatggaa
     4561 tcgttgcacc atgaaacatg ttggggaagc ttgtaacatg tctcatcaaa aagaaatctg
     4621 ccgggtttcc aataacaccg ccgatatccc gtccaaaggc caatccgaat ccaccaaata
     4681 cccacataat cgttgttatc ccaacggaga gaaaagattg agacatgatc gttagtatat
     4741 tattcttccg tgctaatccg ccgtaaaaaa aagctagacc aggagtcatc aggcaaacca
     4801 ttgctgtaca taaaatcatg aatgctgtat ctcctgtatt gataggtaat gtattcattt
     4861 ttttcgcctc ctttctacga ttacgagtat aaaagaaggt gtagttgttt ttttcttctg
     4921 tttttttgaa ttaatacttg catttataaa a
//
LOCUS       DAMEET010000125         4925 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.129, whole genome shotgun sequence.
ACCESSION   DAMEET010000125 DAMEET010000000
VERSION     DAMEET010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.129"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            80..967
                     /locus_tag="PB780_002436"
     CDS             80..967
                     /locus_tag="PB780_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDE0734452.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1107..2033
                     /gene="cysK"
                     /locus_tag="PB780_002437"
     CDS             1107..2033
                     /gene="cysK"
                     /locus_tag="PB780_002437"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDE0734453.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2269..2715
                     /locus_tag="PB780_002438"
     CDS             2269..2715
                     /locus_tag="PB780_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDE0734454.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2988..4340
                     /locus_tag="PB780_002439"
     CDS             2988..4340
                     /locus_tag="PB780_002439"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HDE0734455.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4511..4798
                     /locus_tag="PB780_002440"
     CDS             4511..4798
                     /locus_tag="PB780_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734456.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1692 a          813 c         1030 g         1390 t
ORIGIN      
        1 aaagtataat ttaattaatt aagtttgttt taagaagaga actttttatt aagttttttt
       61 attttgagga ggaatttgaa tgcaagagct gaagacccgc agtgcccggc accatagacc
      121 taaaagaaaa atggtgatca ataaaaaagc gattgctcct ttaattggaa cgagtctttt
      181 agttgggccg atgatggtta cagcggtacc gcaacatgtt catgcagatg aacaaggatc
      241 tcaaacagtc gctgatcctc aagcgtttat cgatcaaatc ggctggtctg cgtcagaagt
      301 tgctgcaagt aatgacttgt atgcttcagt catgatcgct caagcgttgt tggaaagtgc
      361 atatggcact tccggattag ctagtgcgcc aaattataat ctattcggtg tgaaaggaag
      421 ctataacgga caggtcgttt atatggcaac caaagaatat gtaaacggac aatgggtaac
      481 gatgaatgag ccattccgaa aatattcttc ttattgggaa tccttccaag accatgcgaa
      541 tgttcttcgt tcaaccagtt tttccacggg cgattatcat tatgctggcg tgtggaaaag
      601 caatacaaca agtttttatg atgcaacagc gtacttgaca ggtcgttatg ctaccgatcc
      661 aaattatggg gccaaattga actcattgat taatacttat aatttaaccc gttttgatac
      721 gccatcaaca ggttcggcta caacaaccac aagtacaaca acaacgacta ctacgacgac
      781 ttcaacgtca tcatcttcaa caacagatgt aaatacaaat aatggtcaga catatacagt
      841 cgtgtcaggt gatacgctat gggatattgc acaaaaattt ggcatgtctg ttgatgatct
      901 aatggcgaaa aatggcatgt ctatgtccag ttattcttta ttagttggtc aagaactaaa
      961 cgtttgattt agataaaaag gcaaagaaat taccatatat ggtatatctt tgtctttttt
     1021 ttgctagtca aagacgaatc actcgccata aatgaacaga ttgtattatg ataagtaaga
     1081 agagcaaaga caaggaggaa ttgaagatga cagctatcta taactcagtc accgaattga
     1141 ttggcaaaac gccaatcgtc aaattaaata aaatcgtacc agaagattca gcagatgttt
     1201 ttgtaaagct tgagtttttt aatccaggag gaagtgtaaa agatcgtatt gctttaagta
     1261 tgatagaaaa agcagaacac gatggtctac tgaaaccagg tgatactatc attgaaccta
     1321 cttctggaaa tacaggaatc ggattgtcga tggtaggagt tgcaaaagga tataaagtaa
     1381 tcatcgtgat gcctgagacg atgagtatcg aaagacgttt attgatgaaa gggtacggcg
     1441 cagagttgat tttgactcct ggtgcggatg gtatttctgg atcaatcaga gaagcagaac
     1501 gtttggcaaa agaaaacggc tactttttac cattacaatt tgaaaatgaa gcaaatcctc
     1561 ttgtgcatga gaagacgact ggtcctgaga tacatcaagc atttggagta aatggcttgg
     1621 acgcgtttgt tgcaggtatc ggtacaggag gaacgatcac tggcgctggc cgagaattaa
     1681 aacgtgtgta tccgaaaatc gaattaatcg gggtagaacc agcagaatct gctattttag
     1741 aagggaaaga agcgggacct cacaaaatcc aaggaatcgg tacaggtttt gttcctaaaa
     1801 cactggatac ttctgtgtat gataaagtcc tttctattag tggagacgaa gcgatggaaa
     1861 cagctagaga agttgggcga aaagaaggga ttttagtagg catctcatct ggcgctgcaa
     1921 ttgctgccgc gctaaaagtc gcaaaagaat taggaaaagg caagaaggta ttagctgtgg
     1981 ttcccgacaa tggtgaacgt tatttatcta cagcccttta ccaagagata taaggcactt
     2041 ggtttttctc cagataggtt tatgtaaaaa acttttaaag atgaaaagaa cacacaaaaa
     2101 tttatgatga gaaaaattat ttgaatagag acgtagaata gtgaacgcga ctgttttacg
     2161 tcttttttca ttaaaataca ataaaattga attttctttt catgttaagt ggattttaaa
     2221 atgtaaaaat agtatgataa gacgagaata acaacgtggg gtgtttacat ggaaaataag
     2281 ttagtcgaag aggttatcga aaagttaaaa aaagagaaca ttcgaatcac acctcaacgg
     2341 tacgcggttt tagaatattt gattgaacat cattcccatc caacggctga tgagatttat
     2401 aaagcacttg aacatcggtt tccaaatatg agtgttgcga ctgtttacaa taaccttcgc
     2461 ttatttactt cgattggatt tgttcaagaa atgaagtacg gcgatgcttc tagccgattt
     2521 gatttcagtt caaagcgtca ttatcatgta atttgtgaaa actgcggtaa gattgttgat
     2581 tttcattacc cagggcttga agatatagaa atggctgcaa gcagactgac tgattttgag
     2641 attaatgaac atcggttgga attgtatgga ctctgtccgg attgtaaaga gtcagcaaaa
     2701 aaggcgcaaa tgtaaaaaca gtttctacaa gtgttatacc taaaaaaagc tagctgtgta
     2761 aaaaaaacgc tagctttttt atttttttgt cttttattca caatcttaga tataaaagtg
     2821 aatattatta aaataagaat cttgtagata ttgggttctt atgcttgaaa aggaatgaaa
     2881 ctacaaacaa acctttgtga attcacaagc attaaactgt tgtatccgtt ttatttctgt
     2941 tatagtagtt ttgtcgaagg acaaaatgtt aatcggaggg accataaatg aaagtagtag
     3001 taattggctg tactcatgca ggtactgcag ctgtaaaaag tattttaaaa aatcatccag
     3061 aagcagaagt gacagtatat gaaagaaatg acaatatttc tttcttatct tgcggtatcg
     3121 cactttatgt cggcggagta gtaaaggacc cagcaggctt gttctactca aatcctgaag
     3181 aattatcttc attaggagca acagttaaaa tggaacacga tgtaacagac gtcgatacag
     3241 aaaataaaac agtaacggca aaagatctga aaacaggaga aacagaaacg gtttcttatg
     3301 ataaattagt gatgacaact ggttcatggc ctattatccc accgattagt ggaatcgatg
     3361 cgaaaaatgt actcctatgc aaaaactaca atcaagcaaa tgaaattatt gcacaagcga
     3421 aagaagcaaa acgtgtagta attgttggtg gtggatatat cggtattgaa ttagtagaag
     3481 cttttgtcga atctggaaaa caagtgacat tgattgatgg attagatcgc atcttgaata
     3541 aatatctaga taaaccattc acagatatct tagaaaaaga attgacagac cgcggtgtta
     3601 ctcttgcgct aggcgaaaat gttactgaat ttatcacaga tgaagaagga aaagtgaaac
     3661 aagtagctac accaacagat acatttgata cagatatggt cattttatgt gtgggcttcc
     3721 gtccaaatac aaaactgatc gaaggaaaag tagatacact gccaaacggg gcaatcaaag
     3781 tgaatgaata catgcagaca agtaatccag acatttttgc agctggagac tctgcagtag
     3841 tcaactacaa tccaagtggc acgcaaaact atattccttt ggcaacaaat gccgttcgtc
     3901 aaggtttgct tgtgggaaat aacttgacag atcataaaat ggcgtatcgt ggtactcaag
     3961 gaacttcagg actttaccta tttggctgga cgatcggttc gacaggtgtg accaaagaaa
     4021 gcgcaacttt gaacgatcta gaagtccaag cgactgtatt tgaagataat tatcgtccag
     4081 agttcatgcc aactactgaa aaagtaatga tggagctagt ctatgaaaaa ggaacgaatc
     4141 gtatcgtagg tgctcagttc atgtccaaat acgacattac acaatcagca aataccatgt
     4201 ctttagctgt tcaaaataaa atgactgtag aagatctcgc actttcagat ttcttcttcc
     4261 aaccacattt tgaccgccca tggaattact tgaacctact tgcacaagca gcgctaggcg
     4321 aaatcgaaac tgcacaataa agaaaaaggg ttgtgacaca attttgtcac gaccctctgt
     4381 ctttttttgt gaaaagtaca attagttgtc tttcttcgaa ttttttgtca caataagcag
     4441 caaagataaa gctgttacat aaaagaacaa gcagctttat ttgattttga aaagaaaggt
     4501 gagaaagaag atgaataaag aatattttgt ccaacaagat gcatttttag aagattataa
     4561 aagtgcttcg aagaaattaa cgatcaatat tgatgagttg gcagatcaag tactctctca
     4621 gttgaatgaa acgcaagagg aatattacac gatgccagca aatgacgcca aagataatgt
     4681 ttctcatcgt tttcctttca gtagacgcat ctactcagat gaaaatgacg gcacattgaa
     4741 tacagagtac acatatgaag gtcagccata tgtgctagaa actgatgatg atctttaaag
     4801 acagtttttg aaaaggccgc tcatccttaa gtgatgacaa aaaatcatca caattaccga
     4861 tgactgtctc tttactatct tttaatcaga aaaaggggtt atgacaagct ttgtcaaccc
     4921 ctttt
//
LOCUS       DAMEET010000126         4723 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.131, whole genome shotgun sequence.
ACCESSION   DAMEET010000126 DAMEET010000000
VERSION     DAMEET010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.131"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            51..746
                     /gene="vanR"
                     /locus_tag="PB780_002441"
     CDS             51..746
                     /gene="vanR"
                     /locus_tag="PB780_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDE0734457.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            724..1878
                     /locus_tag="PB780_002442"
     CDS             724..1878
                     /locus_tag="PB780_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HDE0734458.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2093..3061
                     /locus_tag="PB780_002443"
     CDS             2093..3061
                     /locus_tag="PB780_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HDE0734459.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3054..4085
                     /gene="vanA"
                     /locus_tag="PB780_002444"
     CDS             3054..4085
                     /gene="vanA"
                     /locus_tag="PB780_002444"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HDE0734460.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4091..4699
                     /locus_tag="PB780_002445"
     CDS             4091..4699
                     /locus_tag="PB780_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HDE0734461.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1457 a          907 c         1109 g         1250 t
ORIGIN      
        1 tataatctga taaattatta agggcaaact tatgtgaaag ggtgataact atgagcgata
       61 aaatacttat tgtggatgat gaacatgaaa ttgccgattt ggttgaatta tacttaaaaa
      121 acgagaatta tacggttttc aaatactata ccgccaaaga agcattggaa tgtatagaca
      181 agtctgagat tgaccttgcc atattggaca tcatgcttcc cggcacaagc ggccttacta
      241 tctgtcaaaa aataagggac aagcacacct atccgattat catgctgacc gggaaagata
      301 cagaggtaga taaaattaca gggttaacaa tcggcgcgga tgattatata acgaagccct
      361 ttcgcccact ggagttaatt gctcgggtaa aggcccagtt gcgccgatac aaaaaattca
      421 gtggagtaaa ggagcagaac gaaaatgtta tcgtccactc cggccttgtc attaatgtta
      481 acacccatga gtgttatctg aacgagaagc agttatccct tactcccacc gagttttcaa
      541 tactgcgaat cctctgtgaa aacaagggga atgtggttag ctccgagctg ctatttcatg
      601 agatatgggg cgacgaatat ttcagcaaga gcaacaacac catcaccgtg catatccggc
      661 atttgcgcga aaaaatgaac gacaccattg ataatccgaa atatataaaa acggtatggg
      721 gggttggtta taaaattgaa aaataaaaaa aacgactatt ccaaactaga acgaaaactt
      781 tacatgtata tcgttgcaat tgttgtggta gcaattgtat tcgtgttgta tattcgttca
      841 atgatccgag ggaaacttgg ggattggatc ttaagtattt tggaaaacaa atatgactta
      901 aatcacctgg acgcgatgaa attatatcaa tattccatac ggaacaatat agatatcttt
      961 atttatgtgg cgattgtcat tagtattctt attctatgtc gcgtcatgct ttcaaaattc
     1021 gcaaaatact ttgacgagat aaataccggc attgatgtac ttattcagaa cgaagataaa
     1081 caaattgagc tttctgcgga aatggatgtt atggaacaaa agctcaacac attaaaacgg
     1141 actctggaaa agcgagagca ggatgcaaag ctggccgaac aaagaaaaaa tgacgttgtt
     1201 atgtacttgg cgcacgatat taaaacgccc cttacatcca ttatcggtta tttgagcctg
     1261 cttgacgagg ctccagacat gccggtagat caaaaggcaa agtatgtgca tatcacgttg
     1321 gacaaagcgt atcgactcga acagctaatc gacgagtttt ttgagattac acggtataac
     1381 ctacaaacga taacgctaac aaaaacgcac atagacctat actatatgct ggtgcagatg
     1441 accgatgaat tttatcctca gctttccgca catggaaaac aggcggttat tcacgccccc
     1501 gaggatctga ccgtgtccgg cgaccctgat aaactcgcga gagtctttaa caacattttg
     1561 aaaaacgccg ctgcatacag tgaggataac agcatcattg acattaccgc gggcctctcc
     1621 ggggatgtgg tgtcaatcga attcaagaac actggaagca tcccaaaaga taagctagct
     1681 gccatatttg aaaagttcta taggctggac aatgctcgtt cttccgatac gggtggcgcg
     1741 ggacttggat tggcgattgc aaaagaaatt attgttcagc atggagggca gatttacgcg
     1801 gaaagcaatg ataactatac gacgtttagg gtagagcttc cagcgatgcc agacttggtt
     1861 gataaaagga ggtcctaaga gatgtatata attttttagg aaaatctcaa ggttatcttt
     1921 actttttctt aggaaattaa caatttaata ttaagaaacg gctcgttctt acacggtaga
     1981 cttaataccg taagaacgag ccgttttcgt tcttcagaga aagatttgac aagattacca
     2041 ttggcatccc cgttttattt ggtgcctttc acagaaaggg ttggtcttaa ttatgaataa
     2101 catcggcatt actgtttatg gatgtgagca ggatgaggca gatgcattcc atgctctttc
     2161 gcctcgcttt ggcgttatgg caacgataat taacgccaac gtgtcggaat ccaacgccaa
     2221 atccgcgcct ttcaatcaat gtatcagtgt gggacataaa tcagagattt ccgcctctat
     2281 tcttcttgcg ctgaagagag ccggtgtgaa atatatttct acccgaagca tcggctgcaa
     2341 tcatatagat acaactgctg ctaagagaat gggcatcact gtcgacaatg tggcgtactc
     2401 gccggatagc gttgccgatt atactatgat gctaattctt atggcagtac gcaacgtaaa
     2461 atcgattgtg cgctctgtgg aaaaacatga tttcaggttg gacagcgacc gtggcaaggt
     2521 actcagcgac atgacagttg gtgtggtggg aacgggccag ataggcaaag cggttattga
     2581 gcggctgcga ggatttggat gtaaagtgtt ggcttatagt cgcagccgaa gtatagaggt
     2641 aaactatgta ccgtttgatg agttgctgca aaatagcgat atcgttacgc ttcatgtgcc
     2701 gctcaatacg gatacgcact atattatcag ccacgaacaa atacagagaa tgaagcaagg
     2761 agcatttctt atcaatactg ggcgcggtcc acttgtagat acctatgagt tggttaaagc
     2821 attagaaaac gggaaactgg gcggtgccgc attggatgta ttggaaggag aggaagagtt
     2881 tttctactct gattgcaccc aaaaaccaat tgataatcaa tttttactta aacttcaaag
     2941 aatgcctaac gtgataatca caccgcatac ggcctattat accgagcaag cgttgcgtga
     3001 taccgttgaa aaaaccatta aaaactgttt ggattttgaa aggagacagg agcatgaata
     3061 gaataaaagt tgcaatactg tttgggggtt gctcagagga gcatgacgta tcggtaaaat
     3121 ctgcaataga gatagccgct aacattaata aagaaaaata cgagccgtta tacattggaa
     3181 ttacgaaatc tggtgtatgg aaaatgtgcg aaaaaccttg cgcggaatgg gaaaacgaca
     3241 attgctattc agctgtactc tcgccggata aaaaaatgca cggattactt gttaaaaaga
     3301 accatgaata tgaaatcaac catgttgatg tagcattttc agctttgcat ggcaagtcag
     3361 gtgaagatgg atccatacaa ggtctgtttg aattgtccgg tatccctttt gtaggctgcg
     3421 atattcaaag ctcagcaatt tgtatggaca aatcgttgac atacatcgtt gcgaaaaatg
     3481 ctgggatagc tactcccgcc ttttgggtta ttaataaaga tgataggccg gtggcagcta
     3541 cgtttaccta tcctgttttt gttaagccgg cgcgttcagg ctcatccttc ggtgtgaaaa
     3601 aagtcaatag cgcggacgaa ttggactacg caattgaatc ggcaagacaa tatgacagca
     3661 aaatcttaat tgagcaggct gtttcgggct gtgaggtcgg ttgtgcggta ttgggaaaca
     3721 gtgccgcgtt agttgttggc gaggtggacc aaatcaggct gcagtacgga atctttcgta
     3781 ttcatcagga agtcgagccg gaaaaaggct ctgaaaacgc agttataacc gttcccgcag
     3841 acctttcagc agaggagcga ggacggatac aggaaacggc aaaaaaaata tataaagcgc
     3901 tcggctgtag aggtctagcc cgtgtggata tgtttttaca agataacggc cgcattgtac
     3961 tgaacgaagt caatactctg cccggtttca cgtcatacag tcgttatccc cgtatgatgg
     4021 ccgctgcagg tattgcactt cccgaactga ttgaccgctt gatcgtatta gcgttaaagg
     4081 ggtgataagc atggaaatag gatttacttt tttagatgaa atagtacacg gtgttcgttg
     4141 ggacgctaaa tatgccactt gggataattt caccggaaaa ccggttgacg gttatgaagt
     4201 aaatcgcatt gtagggacat acgagttggc tgaatcgctt ttgaaggcaa aagaactggc
     4261 tgctacccaa gggtacggat tgcttctatg ggacggttac cgtcctaagc gtgctgtaaa
     4321 ctgttttatg caatgggctg cacagccgga aaataacctg acaaaggaaa gttattatcc
     4381 caatattgac cgaactgaga tgatttcaaa aggatacgtg gcttcaaaat caagccatag
     4441 ccgcggcagt gccattgatc ttacgcttta tcgattagac acgggtgagc ttgtaccaat
     4501 ggggagccga tttgatttta tggatgaacg ctctcatcat gcggcaaatg gaatatcatg
     4561 caatgaagcg caaaatcgca gacgtttgcg ctccatcatg gaaaacagtg ggtttgaagc
     4621 atatagcctc gaatggtggc actatgtatt aagagacgaa ccatacccca atagctattt
     4681 tgatttcccc gttaaataaa cttttaaccg ttgcacggac aaa
//
LOCUS       DAMEET010000127         4704 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.132, whole genome shotgun sequence.
ACCESSION   DAMEET010000127 DAMEET010000000
VERSION     DAMEET010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.132"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            168..656
                     /locus_tag="PB780_002446"
     CDS             168..656
                     /locus_tag="PB780_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734462.1"
                     /translation="MELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTAAEL
                     QKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFP
                     QLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEK
                     AN"
     gene            715..1155
                     /locus_tag="PB780_002447"
     CDS             715..1155
                     /locus_tag="PB780_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734463.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1167..1835
                     /locus_tag="PB780_002448"
     CDS             1167..1835
                     /locus_tag="PB780_002448"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDE0734464.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1899..2522
                     /locus_tag="PB780_002449"
     CDS             1899..2522
                     /locus_tag="PB780_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HDE0734465.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2578..2964
                     /locus_tag="PB780_002450"
     CDS             2578..2964
                     /locus_tag="PB780_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HDE0734466.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3177..4568
                     /locus_tag="PB780_002451"
     CDS             3177..4568
                     /locus_tag="PB780_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HDE0734467.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1562 a          782 c         1000 g         1360 t
ORIGIN      
        1 ttttaaaaga tactctgatt gagcgatcta catttctata tgttcaattg caggaaaata
       61 aaacattgct gcatttttca ccagagttcc atctagaggg gctaacatta ggagaaatct
      121 atcaagcaga agggctgtcg gctgttttgc acttttttaa ggtagaattg gaattaccag
      181 taaaagatta tatcgagctt tctcttgaat cttgggatcg agcagtggtt gaactgtttc
      241 cagaaggttt aatgatcgat acaaacgatg ggaagatcca tctgactgca gcagaactgc
      301 aaaaacaaat gcgttatcaa atagatgacg aaaattcttt ttctattttt agacgtcagc
      361 aaaagattct aaaaagcttt ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga
      421 cacagctgtt gaaaaaatat ccaggtctgc ttcacacgtc tattcaattt cctcaattga
      481 ttacgatgat ccatcgtttt atacgtatgc aacagctacc atctaagaag ctgtttcttc
      541 cattgacgga cacatttcga gtagtcaaca gagaagataa aaagaaagtg attgtgattg
      601 actttaccag aaatcggaat gtccttcacc aagttgcaat ggaaaaggcc aattaaggct
      661 ttttcttttt ggattttttt gtcataataa atattagatg atggaggaga taatatgatg
      721 caaacatggt tagaaaggct aatacgattt cagcagaact atcacaaact tatgcaggga
      781 agatatgcga aatttgatca gttgaatcgc ggtctattga tcgctgcgct tgtctttagt
      841 ttgctaagtc gttggttgcc ctatagaatc ggacaattgt tgttcatcgt ctttttcgga
      901 tggatgatat ttcgtttcct atcaaaaaaa atctacccca gactaaatga gaatcaacgt
      961 tatttgaaat atcttgatag agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa
     1021 gtcaaaatgg atcgaaaaag ttatactttt tttgagtgcc cgaattgtca tcaaaagcag
     1081 cgtgcaccta aaggaaaagg acgaatacgt gtgacatgca aaacatgtgg tataaaattc
     1141 gaaacgaatg tttaagggag aaaagaatga aattagtatt attacgtcat ggcgaaagcg
     1201 aagcaaattt tgaaaattac tggacaggat ggctagatgt cgccttaaca gaaaaaggaa
     1261 aagaacaagc agcagttgcc ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg
     1321 tttatacctc tgttttgaaa cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg
     1381 gtcagttatg gcttcctata ataaaaacat ggcgtctaaa tgaacggcat tatggggatc
     1441 ttgtgggtaa aaacaaagat aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat
     1501 ggcgcagagg atatacggaa gttccgccgt taaccgcaga aaatagattt gaccgcagat
     1561 atgatcactt ggatccgcat ttgatcccat acggagaaag tctggaaatg acagtgaatc
     1621 ggatcattcc cttatggcaa gatcatctag caccaaaact aaaagatcgg caagatttac
     1681 tagttgtggg tcatggtaat agcatccgag cactgacaaa atatttagaa gatgttcctg
     1741 aagatcaaat ggatacgatc gatatcccaa atgcacaacc aatccagtat acattagcag
     1801 atgatttgac aattctggac aaaaaaatac tttaaagaac acctttatct aaaagtgaga
     1861 gatagaaaaa agaagcaaag gaatggaaca aaaggaacat gaaaaattat atatgggact
     1921 ttgatggtac attgtttgat acgtatcccg caatggtaga tggtgcatgg caagcactaa
     1981 aagattttgg tatcagtatg gacaaaaaag aaatctactt caaaatgaaa aagtattcta
     2041 ctagttattt gatcaatgaa tcaaacttga atgccagaga gtttaatgaa ttatttcacc
     2101 gatatgaaaa agaaagtacg gaagtttccc gtccatttcc agaaacgaaa caagtactgg
     2161 aaatgctaaa agacaacggc ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa
     2221 catggggcct gttaaaagaa catcgattgg cacatctgat tgaggaagta gtagggatcg
     2281 atcaggactt tcctcgtaaa cctgatccag catctttaaa ttatttgatc gatacttttc
     2341 atttagaaag aacagacacg atgatgatcg gcgatcgtcg tttagatatc gaagcaggga
     2401 aaaatgcagg agtcgctact tgtctttacg acatcgatca ttttctgggt gagatcccgg
     2461 cagattatgt ggtaggaaat ctgaatgaaa tccttacctt gcagagcatc attaaaaagt
     2521 agtatcccct ttcaaaggag atatgttata attttcataa aggagtgata aaattatatg
     2581 gaaatcagaa gacaaaaaga agttgtagaa gcatttcatt atgatatgag attgccgaac
     2641 caagaagtag aaacagattt gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac
     2701 tatccgaaag aacatagcat cgtaggagca cgcttagagt tccgtttagt gtttgataca
     2761 tatgttttat ctggaagtgt gagtcagatc aatcatatcc tcaatcggaa aattgaaggt
     2821 caaaatgatg tcacacaaga agaagtggac gagctagtag caccgttatt cgatatcgta
     2881 cgccgaatgt cttatgaggt gacagagatc gccttagatc agcctggggt aaaattgaat
     2941 tttcaatcag aaaaacaaga atagacagta aaaacatacc tgcgatagat taaagctatc
     3001 acaggtatgt ttttttatta ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg
     3061 tctaaaatat aaaagtcact gaaaaaaatt ttttcaggag gatggttgaa gaatcattct
     3121 cgggaacctt tacttccttc gatggacttg tcattaataa tatgaggagg agaaaaatga
     3181 caacactcat cacagtttta gtggttctag tttttgcggc attattatat gtgttctatc
     3241 gtatgcaaaa gcgccataca aaattttcaa cacgcgtctt tacagcatta ggagttggta
     3301 tcgtctttgg cggtctgctt caattgatct ttggtgtaga cgacaaagtc gttactcaat
     3361 caatggaatg gatcgggatt gtaggtcaag gatatgtatc attcttgcaa atgctagtta
     3421 tgcctttagt gtttatttcg atcattggtg cattcacaaa aatgaaagtt tctgaaaagc
     3481 tagggaaaat tagtgcgact gtattgactt ctttacttgg aacaacagct attgcagcat
     3541 tgatcggaat tggttcaaca atgctgttcg gactccaagg agctaaattc acacaaggag
     3601 ctgcagaaac agcacgaatc tctgaactgg caacacgtca ggaaacagtt gaaaacttat
     3661 cgattccaca gcagatttta tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta
     3721 gtcgtgcaac aagtacgatt ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc
     3781 taggcgtgaa aagaaaagcg ccaaaagaag cagcattctt ttcagattta gtcgacagtc
     3841 tgtataaaat cattatgcgg atcgtgacgc ttgttttgcg tttgacacct tatggagtat
     3901 ttgctttgat gacaaacgtt ttggcaacaa gtgattttgc agctatcgtc aatttaggta
     3961 aatttgtcgt tgcttcttac gtagcattgc ttgctgtttt gattgtccac tcactgattt
     4021 tgattggtgc aaaagtcaat ccaatcaact atttcaaaaa agcctttccg gtattgagct
     4081 ttgcgtttac ttcacgttca agtgctggag ctttgccatt gaatatcgag actcagacaa
     4141 aagctttagg agtagatgat gcaacagcta atttctcagc aagttttggt ttgtctattg
     4201 gacaaaacgg ctgtgctggc gtttatccag ctatgttggc aacgatcgtc gcaccagcag
     4261 ctggagtgaa tgtctttagt ttggaattta ttttgatgct tgtggcaatt gtaacaatca
     4321 gttcttttgg tgtagctgga gtcggtgggg gcgcaacatt tgcttcactg atcgttttag
     4381 gggcaatgaa cttgccagta gcaatcgttg ggttagttat ctctgttgaa ccattaatcg
     4441 atatggcacg tacagcagta aacgtcagcg gtagcatggt agctggtatc gtgacaagtg
     4501 cacgcatcaa agatttagat cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga
     4561 atgcatagtc aaaaagaggt tgtgatagaa gtggtcagct ttaagaaata agaagaagtt
     4621 tcctaaaaat agtttctcaa atttttgtga aatttcggct tatttccgag aagctacttc
     4681 tgaaacaccg tgaataagag gttg
//
LOCUS       DAMEET010000128         4687 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.133, whole genome shotgun sequence.
ACCESSION   DAMEET010000128 DAMEET010000000
VERSION     DAMEET010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.133"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            107..1248
                     /locus_tag="PB780_002452"
     CDS             join(107..335,335..1248)
                     /locus_tag="PB780_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDE0734468.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            1424..3631
                     /locus_tag="PB780_002453"
                     /pseudo
     CDS             1424..3631
                     /locus_tag="PB780_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
     gene            3711..3965
                     /locus_tag="PB780_002454"
                     /pseudo
     CDS             3711..3965
                     /locus_tag="PB780_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4040..>4687
                     /locus_tag="PB780_002455"
     CDS             4040..>4687
                     /locus_tag="PB780_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
                     /protein_id="HDE0734469.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWVQEYSKVLYYLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVK
                     YLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVST"
BASE COUNT         1590 a          750 c          936 g         1411 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc gtagaatcta actactaaga aaaggacgtg tccacgatgg caaaagccaa
      121 gaaaaaattt gatgaagact ttaagaagat gatcttagat ttaaaccagt ctggccaatc
      181 ggtcgaagaa ctggcagcgc aatatggtat cgctacacaa acgatttatc gttggaagaa
      241 gcttcataca aagaatgtag caaccggcat gactgaggca gaaatcctag cgatgaaaaa
      301 ggaaatggcc cgcatgcagg aggaaaacac tatcctaaaa aaggctttaa ccatattcgc
      361 tcaaaagtaa aaatgagtga tgtcttcgaa tttattcaat caaatgttca tgatcatgac
      421 gtaaaacaaa tgtgtaccgt tttggaagca ccgaaatcca gctactatga ctggaagaaa
      481 aagaaacctt ccaagcgcca atcagaaaac aaacgcttaa accgcttgat ttcaggaatc
      541 tactttgaaa acaagggtat ctatggtgcg cctaaaattc ataagattct tgttggccgt
      601 ggcgagacct tgtccttaaa gaaagtacaa atactgatga gaaaattagg cttgcgttca
      661 gtcaccctca agaagtacaa gcctggcaaa caagcgaaag ttaaaagtga aggacgtaaa
      721 aacctgttaa accaagattt taccactacg tcgatcaatc aaaaatgggt gacagatatt
      781 acctatattc ataccctaaa agatggctgg acctacctct ccactattca agacctccac
      841 acaaagaaag tcatcggttg gaagtttggc aagcagatga cgaaagaatt ggttatcgaa
      901 acccttgaca atgcgctctt aaaccaaaca ccctccgaga acttgatcat ccactcagat
      961 ttgggttctc agtacactag cgaagcctat gaggcaaagc tgaatgaatt gaacattcgg
     1021 cactccttca gtcgtaaggg ctgtccctac gataatgccg gtatcgaatc gtttcacgcc
     1081 tcactcaaaa aagaagaagt ctatccatca aaagcgtatg aaaactttga agctgcttat
     1141 ttagcgttat ttcaatacat tgaaggattt tacaatcgca agcgaatcca tagtagtatc
     1201 aactatttaa caccaaatca gatggaggag ttggcattgc aagcttagtt cctagctacc
     1261 acttacattt ttttggcgag atttccacag ggtttcaact tcatcgcttc gatcagcggt
     1321 caaacggttt tggtttgacc gctggtcgaa acgatgagat actcacccgc tggaatctcg
     1381 aaaaaattct cacaaatcgt gtccgaggta ttgactcaaa tccattacta atctagtagc
     1441 taattttgga cctaccactg aaaaaatgta cgataatcag gttactccta aatggggcaa
     1501 aaatctacct aaaaatattg tatttcttat tcaaggtgcc ttttggtgta aggggattaa
     1561 tccaggtggc ttcgatgggg tatatactcc atatttagat actgctgtaa aagagttgca
     1621 gactgatgca gggtttaatg gaagcgctgt aactggagtt ttggatgcga aatgggccaa
     1681 agcgttattt gatatgagtg cttttgttct tgttcccggc ggagatagta aaattagaaa
     1741 aatgcagcaa tggttaaatg taaattattt agaatacacg ggaataatgc cttgtgatgg
     1801 tatttatcaa agaaattcaa atcaagcatt aatttttgca ttacaagctg aactaggata
     1861 ttctcccagt gaagcaacag ggtcttatgg taatgggaca actagtaaaa cttatcctgt
     1921 gtctgaaggt aactcgggaa actatgtaag aataattcaa tatggattat atgttaatgg
     1981 ttattttgaa gacggtacat tcgatggtat tttcactaaa tatatgggtt tagaagtact
     2041 ggcattccga aaatttatgg tattgcctga atatactgaa atagcagatg ttgttgtaat
     2101 aaaaggacta ttgtctagcg ctggagatat tagaagatct gcagatggcg cagatacttc
     2161 tacgcaatta actagatctc aaatccaaac attggttgat aatgatataa aaatagttgg
     2221 tagatatctt actggtactg ttggaattgg aaaagatgaa cgaaataagt atctaactac
     2281 agaggaattg aataatattt tttctaaaaa tttatctgtg tttcctatct atcaagatgg
     2341 aggtgcagca ttagcttatt ttacttatga tcgtggctta tcagatggta agaaggctat
     2401 agatgcagca aaaaatctaa atataccatt aacaacagtt atttattttg ctgttgatct
     2461 agacatgtta ggggaagaaa ttcttgcata tgctggagaa tattttaagg gggtatctgc
     2521 tgtaatgtca tatagcgggt atcaaacggg tgtttacgga acaagaaatg tttgttctca
     2581 aataatcaat aacgggtatg cttcgtttgc atttgtttca gatatgtcga ctggttatag
     2641 tgggaattta ggttttccta tgcctagaca atggtctttt gatcaatttg ttgaatttac
     2701 cataggaagt ggcaacggag ctgttggtat cgatttaatc gctacatcgg gtagagatag
     2761 cggatttaat gagttatcaa atgatactaa taatgataat tacattgcca aatacaatca
     2821 gaaggttata aatcagatgg tacgtgcgta tcaatatcta tcacaagctg gtcttgataa
     2881 tccttggaac cctcatttga cattttatag atatgtaaat tattctggtt tgtcatggga
     2941 catcatttct tctcctgtaa cagagcatga tagaaaaatt tatgatgaat ataggaataa
     3001 attaactaat agcgagggcc tgtataatta ttttatagat cctaatactg gttcaataat
     3061 aggtttgcct cacttaattg tgacgttaca atcacagatg tttctagccg acacaatcaa
     3121 tgatagtgtt tctgactttg caggttggtt aggagattta atgacttgct ggggtgaagt
     3181 taagaaatta ggaatacaaa tggagcaagg tgtatttgca ttagtaggta cggctgcagg
     3241 tggaacattt agtctagaag atttatacca agatgttgac gcggtaaatg tttatacctt
     3301 agcaaaaaga aattctgatt caacagcttt atcggcttta aaaacgtact attgtgataa
     3361 gggtgctatt gatagattta atcaatttgt taagaacaga tttggttctt cagataaaat
     3421 cgcttcagta acaaatagtt tattagaaaa tgacgaaaca gttgatatct taggtgtagg
     3481 gataatttca tttttcattg ataaaatagt taataattca cttgggacag attttactta
     3541 tcttgatttt attggagatg aggggaaagt tgcaaatgga ttttctgaaa aaattattca
     3601 atttcaagaa aatagaagta aacttaatta agaatcgttt actatttaaa cagtgtcctt
     3661 ttccttttta aatatatacg ataaaataag tatatgaatt gtaaaaggac atgatgatta
     3721 tggaagaaat agaatggaat aatctgactc aaaataataa aaataattct ggatgtggaa
     3781 ttatctttat gtttgttata gttttgggaa tagtagtact tttaataact gtcaagatag
     3841 tagattactt tgcgaatact cctgaagaac aaatacagag aaaacttcct gagtttgaaa
     3901 gagtaccacc ttatgatcca agagataatt catttcaagt aaaaaaatta aaagagtctc
     3961 caaatgggtt ctgttgcaaa gttttccaaa aaatctattt tagtgtaaaa ttgagaaaaa
     4021 agacagagag gacagagtaa tgaatcattt taaaggcaaa caattcaaaa aagacgtcat
     4081 tattgtcgct gttggttact acctgcgtta caatctaagc tatcgtgaag ttcaggaatt
     4141 gttatatgat cgtggaataa atgtttgtca tactacgatt tatcgttggg tgcaagagta
     4201 cagcaaagtc ctctattatc tttggaagaa gaaaaataga caatccttct attcatggaa
     4261 aatggacgaa acctatatca aaattaaggg acgttggcat tatctttatc gtgcaattga
     4321 tgcggacggc ttaaccttag atatctggtt acgaaagaaa cgggatacgc aagcagccta
     4381 tgctttctta aaacgactcc ataaacagtt tggtgagccg aaagcaattg tgaccgataa
     4441 agcaccttct cttggctccg cctttagaaa gttacagagt gtgggtttat atactaagac
     4501 agagcaccga actgtgaagt atcttaacaa tttaatagaa caagaccatc gacctattaa
     4561 acgacgaaat aaattttatc aaagtctccg tacagcctct tccacgatta agggcatgga
     4621 gaccattcga ggaatatata aaaagaaccg aagaaatgga acgctcttcg gcttttcggt
     4681 gtctact
//
LOCUS       DAMEET010000129         4680 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.134, whole genome shotgun sequence.
ACCESSION   DAMEET010000129 DAMEET010000000
VERSION     DAMEET010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.134"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            189..419
                     /locus_tag="PB780_002456"
     CDS             189..419
                     /locus_tag="PB780_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HDE0734470.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            516..1817
                     /gene="murA"
                     /locus_tag="PB780_002457"
     CDS             516..1817
                     /gene="murA"
                     /locus_tag="PB780_002457"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDE0734471.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1830..2006
                     /locus_tag="PB780_002458"
     CDS             1830..2006
                     /locus_tag="PB780_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDE0734472.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2141..2437
                     /gene="yidD"
                     /locus_tag="PB780_002459"
     CDS             2141..2437
                     /gene="yidD"
                     /locus_tag="PB780_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDE0734473.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2622..3089
                     /locus_tag="PB780_002460"
     CDS             2622..3089
                     /locus_tag="PB780_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDE0734474.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3174..4058
                     /locus_tag="PB780_002461"
     CDS             3174..4058
                     /locus_tag="PB780_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDE0734475.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4172..4408
                     /locus_tag="PB780_002462"
     CDS             4172..4408
                     /locus_tag="PB780_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HDE0734476.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4496..4576
                     /locus_tag="PB780_002463"
     tRNA            4496..4576
                     /locus_tag="PB780_002463"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4530..4532,aa:Tyr,seq:gta)
     gene            4583..4654
                     /locus_tag="PB780_002464"
     tRNA            4583..4654
                     /locus_tag="PB780_002464"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4615..4617,aa:Gln,seq:ttg)
BASE COUNT         1580 a          731 c         1025 g         1344 t
ORIGIN      
        1 atttggaata agggactgcg acaagacttt tgtctcagtc cctttttttg catattttta
       61 gcatttattt cctaaaaaat gtaaggggat tcctatattt ttaagtatac ttgtttttcc
      121 ttttatacat tttatggtat tatcatttta ttagttatta taaaacaatc gttagaaagg
      181 acgtcattat gcaagtatac ggaattgatg cggtgcttcg aatcgtgagt catttggcgt
      241 ttatctactt agccttctgg tctttacgtt cgttacgcat tgaaactttt ttcagaccaa
      301 tgcataatac gcaaattaga atggcaatcg tacttttttc aattttttta gggtacactg
      361 caagtaattt tttcttagaa gttatcgctt tatgccgaaa tcttttcgtt agcctgtaaa
      421 ttttaaaaaa aggtactatt ttaaaagcaa ggatgttata atagaaaaga ttttgagtaa
      481 gtgtatgctc attggaattt tttggaggga acaacatgga agagatcatc gtaagaggtg
      541 gcaatcaatt aaatggaaca gtaagaattg aaggagctaa aaatgctgtt ttgcctattt
      601 tagctgctag tttgttagca gaagaaggta ttacgacatt agacaacgtt ccgattcttt
      661 ctgatgtctt cactatgaat caagtgattc gtcacttaaa cgtagatgta gattttgatg
      721 aacaaaagaa tcaagtaaca atcgacgctt caagacaatt agaaatcgaa gcgccttatg
      781 agtatgttag tcaaatgcgt gcttcaatcg ttgtgatggg tccgctactt gccagaaatg
      841 gacatgcaaa agtagctatg cctggtggat gtgcgattgg taaacgacca atcgatttgc
      901 atttaaaagg atttcaagct ttaggagcaa aaattattca aaaaaacggc tatatcgaag
      961 caattgcgga tgaattgatc ggtaacacga tttatttaga tttccctagt gtaggtgcta
     1021 ctcaaaatat catgatggct gccgtaaaag caaaaggaac aaccatcatt gaaaacgtag
     1081 ctcgcgaacc agaaatcgtt gatttagcaa acattttgaa taaaatgggc gctcaagtct
     1141 atggagctgg tactgaaaca atgcgtattg aaggcgtgga tcatttacat gcagtcaacc
     1201 actcaatcgt acaagatcgt atcgaagcag gtacctttat ggttgctgct gcaatgacac
     1261 aaggcaatgt gctgattgca gatgctatct ctgaacataa ccgcccattg atttctaaat
     1321 tgatcgaaat gggtgcagaa atcattgaag aagaaggcgg agtccgtgtc atcggaccaa
     1381 aacatatctt acctacagat gtaaaaacga tgcctcatcc tggtttccct acagatatgc
     1441 aagctcaaat gacagctatc caattagttg cagaaggtac aagtgtagtg actgaaacag
     1501 tgtttgaaaa tcgtttccaa catttagaag agatgcgccg tatgaacgct catgtcaaaa
     1561 tcgacggaaa tgtagcaatc atggatggaa accatgaatt gcaaggtgca gaagtatatg
     1621 caacagactt gcgggcagca gcagcattag ttttagctgg cttgaaagca aatggaatca
     1681 cacgtgtgcg taacttgaat tatctagacc gtggatatta taatttccat atcaaactac
     1741 agcaattagg tgcagacgtt gaacgtgtag acatggatca aacttctgct gaaaaaacag
     1801 cacaaacgat tgcttaagga agtggctgaa tgagtaacca acgatatatc ttattaactt
     1861 tgataaagat tttaatggtg atcttattgc ttatccttct ttttgtggcc ggaacgatga
     1921 tcggatatgg tgtcatcggt ggcggcaatc catttaaagt atttcagcca agtttgtgga
     1981 ttcatattcg agactttttt cattgataaa taagaatcac agtggttcta gtcgatatga
     2041 agtgagccat gaagcagaac atgataaaag aaaacagaag ggaacgttga cgttagtcgc
     2101 gatccctttt tttagtaaaa gaaaagaagg aatctaattg atgaagaaga tattgattta
     2161 tttagttaga ggatatcagc gctttatttc gcctgcattt ccacctagct gccgttatta
     2221 cccaacctgt tccaattata tgataaaagc aattcaaacc catggcgcgc taaaagggac
     2281 ggtaatgggg actgcacgga tcttacgctg tcatcctttt gtcaaaggcg gtatcgatta
     2341 tgtaccattg acattccgat taacgcgaaa taagaaagat aaaaaagaac cagattataa
     2401 aaaggttgtg aaaaatacgg gtagcactga gcgataaaga aacaatttgg aaaatagctt
     2461 cttatatttt gcaaaattgt gagttattgc cgaagtgttg gattttgaac accgtcaaaa
     2521 aaaaggttgt gaaaaatacg gctagcactg agcgataaag aaaaagagga aatttattgt
     2581 caaagtaaca actccgcaac tacttatatg ggggacaaaa aatgatcgaa gaactaaaca
     2641 agaaagattt accatggtcc ttgttattag atgcagatcc tgataaagaa aaagttcaag
     2701 tatatgtttc cagagggagt ggctggatat ggaaagaaaa agaaaaaaca ataggtgtac
     2761 tgatctatgt ggcaagggaa aaagaatacg aaatcgtcaa tgtagcagta accccaacgt
     2821 atcaaggaaa ggggattggc ggaaaattac ttgagacagc ttttcagaag ctttcacaac
     2881 tcgcttccag tcaaactcag atcatcatac gaaccggcag tactagcagt gcggcgttac
     2941 atctctatca aaaaatgggc tatgtggaga ttggtcgagt caaagactat tttatccatc
     3001 attactctga gcccattttt gaaaatggag atcaattaag ggatcaagtg attttagcaa
     3061 ttcacttacc taaaaatagc tttaactaat aaaatttcaa ttcaagaaaa tgctttcaaa
     3121 tgctaaatag atacattaca atagctttag caaacaaaaa ggagaatatt tttatgggaa
     3181 tattagtttt tgattttggt ggatcggctg tcaaatatgg ttgttgggat ggcaaagaga
     3241 ttaaaggtaa gggacaattt gcaacgcctg aatcttggga agaaatgaaa gcgcaattat
     3301 ttcaagttta taaaaaaata ctcgtttctt ttgaagtcga aggaattgga atcagctctc
     3361 ctggagtagt gaataatgag aaacaggtga ttgagggaat cagtgcgatt ccttatattc
     3421 atggatttaa tattttccga gatttggaaa ctctgtttca acttccagta acgattgaaa
     3481 atgacgccaa ctgtgccgga atggctgaat tttatgaagg tgctgcccgc gattatcaag
     3541 atgttgcttt tgttgtggca ggaactggga ttggcggggc gttgttcacg aaaggacaaa
     3601 tcaataaagg ggcacatctt tacggtggag aatttgggtt gatgttctta aatggcgata
     3661 agacattcag taaacttggt acagcggtac agatggcttg gcgttattgt gaacgcaaag
     3721 gtctcgataa aaatgcttat accggaaaag atgtctttga attagcagaa actggagatg
     3781 caatagcaaa agaagaagtt gagagttttt atacgtatct tacaaaggga ttattcagta
     3841 ttcaattttc gtttgatcca gaagtgatcg tattaggcgg cggggtttct gcaaaagaag
     3901 ggctcattga tgagatcaaa agccgaatga agaagctgac agaacaattc gatctgcatg
     3961 atttcgaacc gcagatttta ttatgtgaat acagaaacga tgctaatctt gtaggtgcag
     4021 cagcaaacta tctcgctgta cgtcaagggg agaattaaga gctttagaga aggtgcagct
     4081 tgcataaaag aagtgaatga aatgaatagg agatttttac ctaaggaatg ttttatttgc
     4141 tgcagtatga taaaatatct gagaggtgaa aatgatgtca aaaaaaaata aagatattga
     4201 agtgcgtata gaagaagtga aaaaagatat caaaggtaag aattatgaag tcactcagtt
     4261 atttataggg aaaaaaatga ttggtgagat cttagcctat ggaccaaaag aatatgaaat
     4321 tttctttggt gaagaagatt ttggaaaaga aaaaagtctt gaaaatgcga tagaaacagt
     4381 gattcgacac tggaatctgc acgaataaaa aaatatcaaa aaaactttca ataaagggtt
     4441 gcaaaaatct ggaaaattag gtattataac taagtcaggt tgatacaaac ctttcggagg
     4501 agtagcgaag tggctaaacg cgacggactg taaatccgtt ccttagggtt cagtggttcg
     4561 aatccactct cctccatttc attggggtat agccaagcgg taaggcaagg gactttgact
     4621 ccctcatgcg ttggttcgaa tccagctacc ccagtatacc acaatcgtaa gctctcgtta
//
LOCUS       DAMEET010000130         4671 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.135, whole genome shotgun sequence.
ACCESSION   DAMEET010000130 DAMEET010000000
VERSION     DAMEET010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.135"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(146..1111)
                     /locus_tag="PB780_002465"
     CDS             complement(146..1111)
                     /locus_tag="PB780_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDE0734477.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFTLSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            1345..2190
                     /locus_tag="PB780_002466"
     CDS             1345..2190
                     /locus_tag="PB780_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDE0734478.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            2381..3568
                     /locus_tag="PB780_002467"
     CDS             2381..3568
                     /locus_tag="PB780_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDE0734479.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            3574..3930
                     /locus_tag="PB780_002468"
     CDS             3574..3930
                     /locus_tag="PB780_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HDE0734480.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            3932..4270
                     /locus_tag="PB780_002469"
     CDS             3932..4270
                     /locus_tag="PB780_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HDE0734481.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    4346..4602
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         1535 a          795 c         1018 g         1323 t
ORIGIN      
        1 ttacgacctc ttggtaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccag
       61 tacaaaaaca tgaacagctg tttttgtttg gccgttctta ttacttgatg cagaacagct
      121 ttttcacaac ctattgaatc gattattaag cgattttttt agaaacaaat tttccatgtt
      181 tgctatgttc tttaattaag aaacgtacgt aatgagcaag aacgtagaag acaactacac
      241 ttgcaaacag acattccgca acttttaaga aatccaatac gctactatag atacctaaac
      301 tgttgaagta atgaacagta gtaaatatag ctgttgcaga gataacaagc gggaatgtaa
      361 aggctgcata gcttggataa aaacgaagct tgaacaaacg tggaagcata gacagcacaa
      421 cgaaatacaa tacttgagat aaagtgaaca atgctacgac aagataaaaa ttaggttcag
      481 caaagttttt aagataacct gttaggcaaa gagaaccagg tgctgcaacg atcgtaatca
      541 gcggacgtgt cggatgagcc atatttctta cgaaaaagac acgtttgatg atgatcggca
      601 ataagaacgc atagcagatc aatgaaatcc aaaagaaaat ttttccaata agtgggaaga
      661 agttcccggc agttacagtg atgaccccaa atccaacgta aatgataaac cagcttggat
      721 aaattgcttc aattgaaaga tcagctttga taacatggtg ataattgaaa taaaagacaa
      781 ggatcacttg gaagataaca gcgcacagcc aaatgaattt gacaattggt gcaatacttt
      841 catatgaaac aaaataagta caaataacca tgagtgacat cgtaaaagtt ggtgaaacgg
      901 aggcgatgat tggatcatgc aacgtttgtt tcgtgtgttc gaataagata aacaatttaa
      961 caaaaatgaa tatcataaga atcatagaga taccgccagt tagattgcct aaagcaacat
     1021 ggtgatagtc tttaaacaga tttccaaggg aagctaaccc aaggatcaac ccgcagatcg
     1081 ggatcggaat aattttcaaa aagttcttca tatataatat tctccttttt atttaataga
     1141 ttttagtacc aagatgaacg aatacattgt aaaatgaata ggaatgtatg attacgagca
     1201 agcagacagc tgattgtctt catctaagtt actagttgga tcagcaactg ttttgttgta
     1261 catttctatt atagatggtt ttttgtatat ttaaatcaaa tagtttttat agtaattatc
     1321 ataaaaagtt atggaagtgg tgccatgttt aagttattaa cgacttttcg agcagtatat
     1381 gaaacaagaa atttttccag agcagcagaa gtactgtttt tatctcaacc agcagtctcg
     1441 aatcaaatca aacagttgga gcgggaattg aatatcgatc tttttcaacg taacggaaga
     1501 aaagagatgc ttccaactaa gcaagccgac ttattatatg aacggacact ggttttgtta
     1561 gaagaatggg aagaaactca gcaaaaattg ctcaatgaac aaaatgaaga agaagtatgt
     1621 cggatcgttg cttcccacac ctttgctgtc tatcttttgc ctcagttgat gaaacatctg
     1681 atcaaaaggt ttcctaaagt gcattttgag atcgaaatca cgaattctca tgaatcatta
     1741 gaacaagtag cgaaacatga atccgatttt ggttttatcg aaaagccatt agagacttcc
     1801 ggcgtacaaa gatactcttt gatggaagac cagctagtca tcgctgggga cccaaaaagc
     1861 aaattgtggc ttgtaagaga agcaaactct ggtgtttttc attacacgca gcgttatttt
     1921 gaagagatga atatccaaga taagaagatg atcgtgaaaa gtaacgatgt aattgttgcg
     1981 ttgctgaagg aaggaatcgg tcgctcgatt ttgtctaagc gctctgttcc agaaaatgtg
     2041 ccaatgactc cgttgaattt ccatcgatat ttttatttca tccaacgaac caatctggtt
     2101 tctgatcagc tgctagagat tgccgaagag atccttcgat acaatgaaag cacagaaact
     2161 tctcacagcg aggagaatct cagctggtag ataattgcat gtatcaaaaa aagttctatg
     2221 atacactaaa gataagcagt aggcttttgt actgcatttg ctcaagcaac tgcacaaacg
     2281 gaaagttaat ttttcccaaa tgtctatgga aagactttcc attatctaac tggaccgcta
     2341 taataatcag cagtatggtt tttaagaaaa gggatgaaac atggaaaaaa aattaaaagc
     2401 ttcgaacgat aatcgtattg attatggcgt gattttacca gtctttctgt tatgtttgat
     2461 tggtctgctc tcattatatg tagcacttag ccatgatccg aacaatccag cagtcttgaa
     2521 aggcgtaggg atgcaagttg tctggtattt agttggagcg gcagcaatcg ttgtcatcat
     2581 gcatttgaat tccaaatgga tctggaaact gactccttac ctttatggtt tggggttagt
     2641 ggtaatggga ctcttgctca aattctatga tcagaattta gctgcttcaa cagggtctaa
     2701 aaactggttt cgattcggtg cgtttacttt ccagccagca gagttgatga agattgcgta
     2761 catcttgatg atggcactga tcgtaactaa acataatacg caagtaaaag aacggacgat
     2821 gaaatcggac ttttggctga ttgggaagct cttgatcgtg acaataccgg ttttagcatt
     2881 gatcatggcc caagatgact ttggaacgat gcttgtcttt cttgcgatct ttggcgggat
     2941 tttcctgatg tctggaatct cttggcgcat catcgcacct gtagtaatct tggcagtagt
     3001 cgttggagca ggaacgatct tcttggttac gactgaaggc ggaagagact tgctttataa
     3061 agttggattc aaatcttatc agtttgcacg tattgattca tggctggacc ctttccatga
     3121 tacgtcagga atgagttatc agccggccca aggacttttg gcaattggta ccggtggctt
     3181 gtttggtaaa ggattcaacg taagcaatat ctatgtgcct gttcgtgagt ccgatatgat
     3241 ttttaccgtt atcggtgaaa acttcggctt tatcggtggt gcattcgtca ttttcttgta
     3301 ttttatttta atctatcgta tgattcgggt gtgtttcgat acgaataatg aattttacgc
     3361 ctatatcgca agcggtttga tcatgatgct gctgttccat gtctttgaga atattggagc
     3421 aaatatcgga ttacttcctt taacagggat cccattgcct ttcattagtc agggaggatc
     3481 ctcgatttta gggaacatga tcggtatcgg gttgatatta tcgatgagat accaaaatga
     3541 agcacctgca agatcttcaa ggaggtaaga aaattgtaca ccttttactg gtacccgaaa
     3601 tgttcaacat gcaaaaaagc caaagcgtgg ttagaccagc atggcgttag ttatcaaacc
     3661 gttgatatga tcaaagaagt accgaaggcc tcaacgattg agacttggct agaaacgagt
     3721 caagtaccaa tccgtcgcct gttcaacaca agcgggatga aatatcggga acttggactg
     3781 aaagatcaag taggcgagtt tacggcttca aaagcgagcc aagtcttatc gacagatggt
     3841 atgctgatca agcgaccact gatcttacga gaggaccagc ttgttgcaat tggattcaat
     3901 gaaaaaattt atgaaggaga actgatataa aatggcaggc aagcatttga agaaaaagga
     3961 caacctttgg atcttattta atggaaaaga atactgtgtt ggtttgacaa atgaagcgca
     4021 agaagaactt ggagacatca cgtttgccaa tctgccaaaa gcaggacaat cgctaaaaaa
     4081 agatgaacct ctgatcgaag ttgaagcgga aaaagcggtc aatgaatttg ccagcccgct
     4141 aacgggtaca gtttcttctg ttaatgataa aatcggtgaa gatatcaata tcttgaatga
     4201 ttcagatgaa atgaatgcgt ggatcttaag tttcaaagat gttgatccaa aagaatttga
     4261 cgaattataa aatcagaaaa tcacttgaaa aaagttgaca ctgattcttc cagttgctat
     4321 aatcataagc aatcctatat gaaacgagaa agctttgaaa agaagagtac atgtcacgga
     4381 cgtttgaaga gagccctgtt tgctgaaaat gggtaacgaa aatgagatgg aagatggtct
     4441 tagagcagag taagcgagcg taagcaaact tactacggga gtgcccgtta cagcgcgaca
     4501 gtatacggaa agaagctaag tccaagtact ggaagaggct gttattgcga agtaacggta
     4561 aatcaaggtg gtaacacgga agttcaagct ttcgtccttt tgcttaaggc aagagggcga
     4621 aggctttttt gtttgaaaaa ttataagaga aagatgtgaa tggcatgtca t
//
LOCUS       DAMEET010000131         4425 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.136, whole genome shotgun sequence.
ACCESSION   DAMEET010000131 DAMEET010000000
VERSION     DAMEET010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.136"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            690..1658
                     /locus_tag="PB780_002470"
     CDS             690..1658
                     /locus_tag="PB780_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDE0734482.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="PB780_002471"
     CDS             1669..2484
                     /locus_tag="PB780_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HDE0734483.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="PB780_002472"
     CDS             2552..3262
                     /locus_tag="PB780_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDE0734484.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="PB780_002473"
     CDS             3298..>4425
                     /locus_tag="PB780_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDE0734485.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DAMEET010000132         4294 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.137, whole genome shotgun sequence.
ACCESSION   DAMEET010000132 DAMEET010000000
VERSION     DAMEET010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.137"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..791
                     /locus_tag="PB780_002474"
     CDS             <1..791
                     /locus_tag="PB780_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDE0734486.1"
                     /translation="CGYSKTEPRHSFGPAIREQYLIHIVLEGEGYYSINDQKYFLKKG
                     QGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYYLKEIGVGKNHLTFKVENLNSFK
                     GLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSASYLNEGIITKKMNPFVRQTIEII
                     RENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIKEYISEIKISIANDLLSSTEYS
                     IQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLGLGIINGE"
     gene            888..3137
                     /locus_tag="PB780_002475"
     CDS             888..3137
                     /locus_tag="PB780_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDE0734487.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3140..>4294
                     /locus_tag="PB780_002476"
     CDS             3140..>4294
                     /locus_tag="PB780_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDE0734488.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
BASE COUNT         1501 a          610 c          790 g         1393 t
ORIGIN      
        1 tttgtgggta ttcaaaaaca gaacctagac atagttttgg accagctatt cgagaacaat
       61 acttaataca catagtgcta gaaggtgaag gctattattc aattaatgat caaaaatatt
      121 ttctgaagaa aggccaagga tttgttatcc caccaggagt atctactttt tatcaagcag
      181 atgaaaaaaa tccgtggtca tatgtatggt tgggtttaag tggcaatctt gtagaatact
      241 atctaaaaga aattggtgtt ggaaagaatc atcttacctt taaagtcgaa aatttaaatt
      301 catttaaagg attaattttt gaaactttat cgtatgatca ggacaatcta gtcagtgaat
      361 tgtccagaca aaaacaagtt tataaatttt tagaactcct cttaaattca gctagttatc
      421 tgaacgaagg gataattact aaaaagatga atccttttgt tcgtcaaaca attgaaataa
      481 tacgtgagaa tgcgtatcaa aaattatctg ttaaagatat tgctgtcagt ctatcgatac
      541 acgaaaatta tttatcacga ttgtttaaga atgatatggg aatatcaata aaagagtata
      601 ttagtgaaat caaaatttct attgcaaacg atctgttatc atcaactgaa tattcaatcc
      661 agcaaatcag tgacaagata ggattctcaa cagtacaatc tttttctaaa gcctttaaga
      721 aagcaaatgg tgtttcaccg ggagtatatc gaaaacgtag attaggtcta ggaatcatta
      781 atggtgaata aactaaacat ttgattttga taaatattcg ttactttaaa cgcttactat
      841 ttatgtgagc gttttttata atgcaataaa gaaacggagg agagcttatg tataaggata
      901 atgaaatgaa tattatttat cgatcagaaa accaacaatt tcatttgttt aatcaggaga
      961 taagttacat ttttaagata tcggaagatg gtaaattatt gcatttatat tatggtgaga
     1021 acttaccaga taatgactat agtcatctaa ttgagatgca tcatcggcct atgacaacct
     1081 atagaaatga aaacgatttg ctatattcgt tagagcactt aaaacaagaa tttcctgaat
     1141 atggtacaac agattttaga catcctgcta tttcattact acagaaaaat ggctctcgct
     1201 tatcagattt tgtctatcaa gggtacgaag tggaagaagg aaaacctaaa ctagaaggtt
     1261 tacctgctac ctatgttgat aataatgatg aaagcaaaac attgaaagtt tatattttag
     1321 attcagttac aaatgttcaa gtagaattat tatatacaat ttatagagat tatcctgtta
     1381 ttactcgtag cgcgcgagtt atgaataaag gaaaagatat tcaaataatc gagaatatta
     1441 gcagtttatc attggattta cccgatgcaa attatgaatg gcaacaatta tctggtgctt
     1501 ggggtagaga aagaactatt aagtcgcgta ctttacaaca aggaattcaa tcaatcgaat
     1561 ctactcgagg tatttcaagc catcagcata acccatttgt tactttaaaa agaaaaaata
     1621 ctgatgaaaa tcaaggagaa gctattggtg cagcactagt ttactcagga aacttcttaa
     1681 ttcaagcaga agtagataca tgggatgtta ctagactaca agttggcatc aatccatttg
     1741 gatttaattg gaaactcaat ccaggagaaa tatttacagc accagaagcg attctggttt
     1801 attcaagtaa gggtttaaat gccatgagtc aaacttttca tcaactattt agaaaacgct
     1861 tagctcgtgg taagtggcga gaaaaagacc gtcctgtctt aattaataat tgggaagcaa
     1921 catattttga ttttgacgaa gaaaagttgg taaatattgc taaaaaagca tttgatgttg
     1981 gtattgaatt atttgtatta gatgatggct ggtttggaga aagagaaaac gattgtgcag
     2041 gattaggaga ttggcatgta aatcctaaaa gactacctag aggaatcggc agcctttcag
     2101 aaaaaattcg tagttttggt atgaaatttg gtttatggtt tgaacctgaa atggtgaata
     2161 aggatagtga gttatatcgc acacatccga attggattat ttctacacct aatcgaaaac
     2221 ctaatcatgg taggaaccaa tatgtattaa attttgggtt agatgaggtt gttgaaaata
     2281 ttttcgatca aatgtgtaaa atcattgatg aatcaaactt agattacatt aagtgggata
     2341 tgaatcgtcc attgactgat gtatttgaca gtcacttgtc agcagatcaa caaggagaag
     2401 tattccacag atatgtacta ggcgtatatc gcttatatga aaaacttata acaaaatatc
     2461 ctaacatact atttgagtct tgttcatcag gtggtgggcg ctttgatcct ggaatgcttc
     2521 actatgcacc tcaagcttgg gctagtgatg attcagatgc aattgaaagg ttaaaaattc
     2581 aatatggaac tagtatgctg tatcctcttt cgtctattgg tgctcatgta tcaattgttc
     2641 caaatcatca aacaaaccgt ataacaccta taaaaactag aggaaatgta gctttttttg
     2701 gtgcatttgg atatgagctt gacttaaatg aattaagtga aggcgatttg aggattgtaa
     2761 aagaacaaat tgccttttat aaaaaatata gaaccataat tcataatggt actttttata
     2821 gactattatc accattcgat gatgataatc aaaccgcgtg gatggttgtt agtcatgata
     2881 agaaaacggc tattgttgct cattataaaa cattaaatga ggtaaatgct ccttaccgcc
     2941 gactgaaatt aaaagggtta gataatgacg gactttataa tgtaggacaa aatagtttgc
     3001 gatctggaat ggaattgatg agagcaggac tagtctgttc agatgcatct agtggacaaa
     3061 taaatgatca gaatcgacgc ccagaaacat ttgattttga ttccaagatt tgggttataa
     3121 cacaggagga aaagtagaga tgaataacca aaaaagctta cttcaaaaag gagcaatctt
     3181 aagtatttca ctaatgttaa caagctctca agcaattaat ggtgttattc cacaaatgaa
     3241 acagtctttg gggatatcac aaagtcaaag tgaactttta ggaactgcac caagtataac
     3301 tgttattttg tttatcttat tatctagtta ctttgccaaa agaataggaa tgaaaaagac
     3361 aataatttta ggtttattat tagctggtat aggaggaatt attccagtat ttacagctac
     3421 cttccaaacc gttcttatta gtcgagtaat attaggtgcc ggtttaggag tatataattc
     3481 attggctgtg acatatatca gctctttata ctctggcaat actagagcaa cgctattagg
     3541 tattagaaat tcaatggaag ctttaggtca gacaatcctt atttttcttg ctggagtttt
     3601 agtgaacatt aattggacgg cttcgttttt agtctatgca attgcgtttc ctgtagctct
     3661 cttattcgcg ctaaaagttc ctgaaattag agatgaaaat agtgacatac ctgaaaatca
     3721 tgtaaaagaa aagatgaatc caatggtttt tgccctagtt ctatttgcaa tcttactcgt
     3781 aatgaatagt attgctattt ctgtacgatt tgcatcaatt gcaactgaaa ttaaaggggt
     3841 aaactttaat gctagtaact atttagctct aatgcctatc ttaggaatac tagcaggatt
     3901 tatgtttgga ccaataaata agtggatagg aaaaggaacc ctatacttgg gaattatatt
     3961 ctttataatt tcaaatttgt taattgctgt gtcaaacggt aatatgacgt tcctgctaac
     4021 gggtttattt ttatcaagta taacaggatc atggtgcttc ccatttatct ttagtaactt
     4081 agataaagtc actacaaaga atacaattaa ttttgctact tcattgattt ttattggctg
     4141 taatattggt aattttatag ctcctattgc tatgcaatta gcacaatttc taacaagaag
     4201 tacaaatttg actgctccat tttttgtatt cgcaggaata tttattgtga tcctatttgt
     4261 aacatttttt gcgacaaata aaaaaaatac taga
//
LOCUS       DAMEET010000133         4282 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.138, whole genome shotgun sequence.
ACCESSION   DAMEET010000133 DAMEET010000000
VERSION     DAMEET010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.138"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(37..1257)
                     /locus_tag="PB780_002477"
     CDS             complement(37..1257)
                     /locus_tag="PB780_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDE0734489.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="PB780_002478"
     CDS             complement(1277..1888)
                     /locus_tag="PB780_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDE0734490.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="PB780_002479"
     CDS             complement(1931..2863)
                     /locus_tag="PB780_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDE0734491.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="PB780_002480"
     CDS             complement(3284..4144)
                     /locus_tag="PB780_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDE0734492.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1219 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag ct
//
LOCUS       DAMEET010000134         4201 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.140, whole genome shotgun sequence.
ACCESSION   DAMEET010000134 DAMEET010000000
VERSION     DAMEET010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.140"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(45..1022)
                     /locus_tag="PB780_002481"
     CDS             complement(45..1022)
                     /locus_tag="PB780_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDE0734493.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            complement(1025..2134)
                     /gene="pdhA"
                     /locus_tag="PB780_002482"
     CDS             complement(1025..2134)
                     /gene="pdhA"
                     /locus_tag="PB780_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HDE0734494.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            complement(2595..4019)
                     /locus_tag="PB780_002483"
     CDS             complement(2595..4019)
                     /locus_tag="PB780_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDE0734495.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
BASE COUNT         1161 a          891 c          731 g         1418 t
ORIGIN      
        1 aatttaaatt gataagccat gactttgttt cgtcccttct tttcttaaaa ttccgcgatt
       61 tcttttactt tgtcttcgat atcttttgca tttggcaacc agacattttc tgcttgtcca
      121 aatgggaaga ctgtatcagg tgcagaaact cgtccgattg gggcttctaa tgaaaggata
      181 gcgcgttctg agatctcaga aactacttgc gcggcaacgc ccgcttgtcg ttgtgcttct
      241 tggacaacaa ctacgcgacc agttttttcc acggattgaa tgatcgtttc tacatctaat
      301 ggcgcaactg tacgaagatc aatgatttca acagatatat tttcttttgc aagattgtct
      361 gctgctttga ttgcttcacg caccattgct ccgtatgtga taatagatac atctgttccc
      421 tcacgagtaa ctgctgcttt atccaaagga acttcgtaag cttcatccgg cacttcttct
      481 cggaatgagc gatatagttt catgtgttct aggaaaacaa ccggatcgtt acttctaata
      541 gaagaaatca atagtccttt tgcatcataa gggttggaag gaatcactac tcgaatacca
      601 ggagactgag cgatcaaacc ttctaaatta tccgaatgca attctggtgt atgaacaccg
      661 ccaccaaatg gcgaacgaat tgtgatgggt agatttctag tgccgctcat tcgatatctt
      721 gtacgagcca tttgagctac tacttcgtcc attgcttcga aaataaaacc aaagaattgg
      781 atttcaggaa ctggacgaaa tccttctaac gcaagaccga aactcaatcc tgcgattcca
      841 gattcagcta aaggagtatc gaatactcgg tcttctccga acttttcctg caatccttca
      901 gttgcccgga aaactcctcc gtttttccca acatcttcac caaaaacaac gacattctcg
      961 tcattttcta gttcaagtgc taaggcatct gtgattgctt ggatcattgt tttttgtgcc
     1021 atgattattt cgactccttc gcttcataaa ttgcaatttg ttctttgatt gattgtggag
     1081 atacttcgaa catgtttttc aagaaatctg aaaccttttg ttttggtact ttgtcggctt
     1141 cagcaatagc tgctttgatt tcttctttag ttgcttcgat gacttgttct tctttttctt
     1201 cagaccaaag tcctttttcc gtcaaatatt tacggaagcg gatcaatgga tcttttttct
     1261 gccaatcgtc atctgtgtct tttgtacggt agcgggttgg atcgtcacca gatagtgtat
     1321 gtggtccata acgataagtc agtgtttcga ttaggactgg tccatttcca ctgatcgccc
     1381 attcccgtgc cattttagat acagtatata ctgcaagcgg atccattcca tcgacttgaa
     1441 taccaggaat cccagctgcg acagcttttt gtgcaagtgt ttttgctgca gtttgttttt
     1501 cacgaggtgt agagatcgca tacccgttgt tttggatata aaatacggca tttgcatggt
     1561 aagcgccagc gaaattgatt gcttcgtaaa aatcaccttg tgatgaaccg ccatcaccag
     1621 tataagtgaa gacgacattt ttcttgtttc gtttcttcaa acctaatgca acaccggctg
     1681 cttgtacata ttgtgcaccg atgataattt gtggtggtag agctttcaat tcttccggat
     1741 aagaattacc tgcagcatgt cctcgagacc ataaaaaagc ttgagataaa ggtaaaccat
     1801 gttttactag ttgaggtaca tcccgataac ctggaagaag aacatcttct ttatcaaatg
     1861 cgtaagcact agccagctga cttgcctctt gtccagcagt tggtgcaaaa aagcctaggc
     1921 gtccttggcg gttcagcgct gttgaacgtt ggtctaatac acgagaccat accatatgag
     1981 tcattaattc aacgagttca tcatcggata aatcaggtac taaatctttg ttaactacgt
     2041 ttccatcttg atcaagtgct tgatatactg ggaaatctgc attgacttca tctagcagtg
     2101 cttggaagtc gatcggtgct tttttgtttg ccatttgtca cacattcctc tctcatctat
     2161 aagaaaatct cttcattctg cgattgaaac agtgaataaa actgtatcac tatttaaccg
     2221 tcttcattaa taaattatca tggatttatg agaaatacaa gctaaaagca caaattttca
     2281 cgatgaaagg aaattatgcc aatccgcata ttaacagcat tagtgaataa aatcacaata
     2341 aggaaagcgt tttatatttc atctgcattt tttctatttt ctaatcaaac ttacaaccat
     2401 ctgtttctat ttaaaaacgt tggtgttata cagttttgtt gcaaacattt attttattta
     2461 cacatcgtga acggacgtac aaataatggt tttttcttag tttctttata tcgtattctt
     2521 tttccgaaaa aaaagaaaca gcttgaaatt taaatccaag ctgttatatt ctttaatcta
     2581 tttactttgt gaaattattc tgttaaccac ttttctagaa ttttcatctt tgaataaaac
     2641 aacggtattt ggttttcatc cagcctactg taattgattc tgattccatt tatatcttgc
     2701 ccaaaaagta gacttggtgc aattccaaga cccatatcta ctactttttc tgctagttcg
     2761 gggtctagtt ttttcccatc ccaagtaatc caggcatagt aaccacctaa aattgaatga
     2821 agcttgaaaa acgattgtga atctccccaa gcgcttatca gtcggctttt ttctttcaac
     2881 ttttttctta catcttgcat tttcaaatca attgcttcat taaatacatt agccactgtg
     2941 atttgtgtaa ggatattcat cgaaaactcc atcatttctt gcgcttgagc tagttgcgag
     3001 ataaaagtct caggtcctat cacccaaccg attttcgttg tttttcctaa aaccctagat
     3061 aacgaaccga cgtataacac attatccgga tcaatcgttc gtataggaag gacttcttct
     3121 tggtttagaa atgaaagatc cgtaaataca tcgtcttcaa taattggaag ccgatactgt
     3181 cgacacaatt ctactagctt ttctcgtcgt ttttgactca tcacttttcc tgtagggttt
     3241 tgaaaattcg ggttgataag cactgctttc aatttatttt tatggatcga ttctttcagt
     3301 ttttctaagt cgatcccttc ttcatccatc ggtatcccaa ataaacgcgt tcctgaagct
     3361 ttgaaaagag gtaatcggta aaaaaaagaa ggtgactcta cagctatccc ctctccagct
     3421 gaaagaatcg tttgtaaaat caaaaagatt gcttgctgac cgccaccagt cacaagaagc
     3481 tggttggggg aaaatgccaa ttcaaattta ctttccaaat accgtttgat tttgttacgt
     3541 aacggagtat agcctaaaag atccatttct ttttcttccg ctaagatttc ttcaagagaa
     3601 taagaaggaa actcaaaatt cggcaaaagc tcatctggca tctcgctagc gtataaatca
     3661 agaaacgatg ttttttggat ctgctttgct tctcttattt tttgctcata ccagtccgtc
     3721 tgctcataac gaggagagat catttgccgc caatcgattc ttggaacagc gaatttcccc
     3781 cattcagtct gattaacaaa ccatccgctg ccacgtttgc tcgttagtac cccaagctcc
     3841 ctaagctctt ccaatgcatg gacaacggtg gaacgattca caccaaaaag ttgccctagt
     3901 tttcgttctg attcaagttg acttccagga ttgatccttc cgttttcgat ttctctcata
     3961 atctgttcca taatttgacg ataaatcggt ccttgttttt tctctacttt ttcccacata
     4021 agcttctctt ttctattgtc tattttttct tttgatttta ccgaatttac gagaaaaaag
     4081 aaaaactttt tttgcaaaac ataagggact gtgacaaaaa tcttatcaca gtcccttatt
     4141 ctgaataatg atgttcaaaa gcttgtatct gcgataataa ttccagccaa acaaaacata
     4201 t
//
LOCUS       DAMEET010000135         4157 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.141, whole genome shotgun sequence.
ACCESSION   DAMEET010000135 DAMEET010000000
VERSION     DAMEET010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.141"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            179..775
                     /locus_tag="PB780_002484"
     CDS             179..775
                     /locus_tag="PB780_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDE0734496.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="PB780_002485"
     CDS             899..2698
                     /locus_tag="PB780_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDE0734497.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2976..3188)
                     /locus_tag="PB780_002486"
     CDS             complement(2976..3188)
                     /locus_tag="PB780_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734498.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            3650..3799
                     /locus_tag="PB780_002487"
     CDS             3650..3799
                     /locus_tag="PB780_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734499.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            complement(4052..>4157)
                     /locus_tag="PB780_002488"
     CDS             complement(4052..>4157)
                     /locus_tag="PB780_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDE0734500.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT         1228 a          706 c          729 g         1494 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctataatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gggatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac aacaaaaaac gcataacctg ccaccgcagg
     2761 tatatgcgtt ttttgtttga cgctcataat gtgtgcttaa ctttctcttg attccttcat
     2821 cgcacgaaca atagctaaaa tagcaggaat tgcagagatc actgcaagaa taattcccat
     2881 tttccctgaa ctttttcttt ttggcatatc ttctcgctcc cttctgtctt tatttatatt
     2941 ataagggagt tcttgcgaat tctcaaaaat actgtttaag aaaccaattc agcaggtgcg
     3001 ctaatatcac agaattttac gagcatgcga tacttgcttt cattgataag ttgatagtct
     3061 cgttggtcat aactttcaac atacacgaca gccgtgttga tatatgtacg ttcaatacag
     3121 ccgattagtt catgagatac tccgatcggt ttgcatatat aagatcttcc tacttctgct
     3181 tttaccattt cattccctct tttctttatt tttattttgt tttttcttta ttttataata
     3241 acaaattacc ttgtatttga aaacgttttt atccattttt ttatttttta ttttgagtat
     3301 ctttctgctt tttagataac tattttttaa cacgatctta gacgttgtga caaaaacgtt
     3361 tgtttatatt cctatgttgt tcgtgcgttt ttttctttat attgctaagt tatttataaa
     3421 gaggtgagca tatgatttat attcaagcaa tagtatttgt aaccttgatg attgggctac
     3481 ttatcggatt aaaagaaaat agctaaacca tttaagaaaa aataaataag gaaaatgata
     3541 aatgcttttt aattgcatca aaaaacgtac gatgctaaac ttggtataag ttcgaaagtt
     3601 taaaggaaac tttcaagaac taaattacat tacaaaggag atgaatttta tgacgtttac
     3661 aaaaacaaat atgcaatacc gcgtagcttt tgatacaact tctaaccgtt ttatggcgat
     3721 tgatgcacaa aacgaaaata acgttgcgtt tggtattaca atcgaacaag cagtccgagc
     3781 attaaacgaa tctagataag gagtgaacca ttccaaatgg cgactgatga gatctcggaa
     3841 aatcaataaa agacgtttgt aacggtaatc cgcgacaaac gtctttttgt attcattaca
     3901 tatatttgga aattgtctct ctttatctct agacctaaac aaaaacacaa gttctaatat
     3961 caaccatctc gttttttatg acaattttgt tagctgtttc gatgataata ttccgttatt
     4021 atctgtataa tatttaatat aatcttaatt gtcaaattaa gttagacaga tcatcaatac
     4081 ttacgtaact caaaaatact tccaaaggtg ttcgataatc cagtgatttt ctaggaatat
     4141 tatttcgttt agatgcg
//
LOCUS       DAMEET010000136         4034 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.142, whole genome shotgun sequence.
ACCESSION   DAMEET010000136 DAMEET010000000
VERSION     DAMEET010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.142"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            73..234
                     /locus_tag="PB780_002489"
     CDS             73..234
                     /locus_tag="PB780_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDE0734501.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..1530
                     /locus_tag="PB780_002490"
     CDS             253..1530
                     /locus_tag="PB780_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDE0734502.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1540..2196
                     /locus_tag="PB780_002491"
     CDS             1540..2196
                     /locus_tag="PB780_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDE0734503.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2196..3572
                     /locus_tag="PB780_002492"
     CDS             2196..3572
                     /locus_tag="PB780_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDE0734504.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3702..>4034
                     /locus_tag="PB780_002493"
     CDS             3702..>4034
                     /locus_tag="PB780_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDE0734505.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1612 a          440 c          704 g         1278 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa agaaatattt tatttagtag tggagtattt
      661 acaattaaag aaggtaaaaa tatagaagaa aatgataaga attcaattat tgttcatgaa
      721 gaatttgcta aacaaaacaa tctaaaatta ggtgatgaag ttgatcttga attactagat
      781 attgaaaaaa gtggaaaaat aaaaagtcat aaatttaaaa ttatagggat cttttctggt
      841 aaaaaacagg aaacatatac aggattatct tctgatttta gtgaaaatat ggtgtttgta
      901 gattattcaa caagccaaga gatattaaat aattcagaaa ataatgaaat tgcaaataaa
      961 attttaatgt attctggtag tgcagaatct acagacttag ccttaaacaa attgaaagag
     1021 cttaaaattg atgagtcaaa gtattttgtt gaaaaagatt ctaatgcgtt tgaagagtct
     1081 ttagagtcag tgagtggaat aaaatatata ataaaagtaa tgacttattc tattatgtta
     1141 ggtgggatgg ttgttctttc attaatcttg attctatggt taagggaaag aatttatgaa
     1201 ataggtatat tcttatctat tggaacatct aagatacaaa ttataatgca atttatattc
     1261 gagttaatat tcatatcgat accaagtata atatcttcct tatttttagg aaatgtatta
     1321 ctaaaagtaa ttgtagatgg attcattaac tcagaggatt caatgatttc cggtggaagt
     1381 ttaataaata atagtagttt tatgtcaaat ataacaacac ttggacaaag ttatttaata
     1441 ttaataagta ttattgtttt atcagttgta tttgcttctt cattaatgtt aatcaagaag
     1501 cctaaagaaa tcttatcaaa aataagttag gagaaaataa tggatatatt agaaataaag
     1561 aatgtagcat atagttatgc aaattctaaa gaaaaagttt tgtcaggagt aaatcaaaaa
     1621 tttgaacttg ggaagtttta tgcgatagta ggaaagtcag gaacaggaaa atctacactt
     1681 ctttccttac ttgctggact tgataaacct caaacaggaa aaatattgtt taagaatgaa
     1741 gatatacaaa ataaaggata tagtaatcat agaaaaaata atatatcttt agtatttcaa
     1801 aactataatt taatagatta tttatcacca attgaaaata ttagattagt aaataaatca
     1861 gcagatgaaa gtatcttgtt tgaactaggt ttagataaaa aacaaataaa aagaaatgtt
     1921 atgaaattat ctggtggaca gcaacaaaga gtagctattg ctagagcatt ggtatcagat
     1981 gctccaataa tactagctga tgagcctact ggtaatctag acagtgttac tgctggagaa
     2041 ataattaata tattaaagac attagctaaa gatagaaata aatgtgtaat agttgtaaca
     2101 catagtaagg aagtagcaga ttctgcggat atcattttag aactaagtgg taaaaagttg
     2161 aaaaaagtaa ataaaatgaa tttggaggtt gaataatgat aaaaaatgca tttgcttatg
     2221 taactagaaa aagtttaaaa tcattaatta tcatattagt tattttatct atgtcaactt
     2281 taagtctcat tagtttatct attaaagatg ctacggacag agcttcaaaa gaaacatttg
     2341 ctaatataac aaatagtttt tctatggaga taaatagaca agtaaatcca ggaacaccta
     2401 gaggtggggg aaatgtaaaa ggtgaagata ttaaaaagat atctcaaaca gatagtatag
     2461 actcctatgt aaaaagaata aacagtgttg cagatttagt tgattatgat attattgaaa
     2521 ctcaagagac tcttgcgaat caatcacctg aaagagcaaa gaattttaaa agaacagtta
     2581 tgttaactgg ggttaacgac tcaacaaaag aaacaaaatt tgtatcagaa gcatataaat
     2641 tagtagaagg aaagcattta gaaaatgaag ataaaaataa aatcttaatg cataaagatt
     2701 tagctgaaaa aaataacctt aaagttggag ataagataaa aataaaatct aatttatttg
     2761 atgctgataa tgagaaaggt gcagatgaaa cagtagaagt agaaattaaa ggtctattcg
     2821 atgggcataa tagcggtgta gtaagtgcag cacaagaact atacgaaaat acattaatca
     2881 ctgatattca tacagctgct aaagtttatg gtaataccga agatacagct gtataccaag
     2941 atgcaacatt ctttgtaaaa ggtgataaga atcttgatag tgtaataaaa gatcttggga
     3001 aattagatat aaattggaga gaatataatt tgattaaaag ttcatcaaat taccctgcat
     3061 tacaacaatc tatatcaggt atttactcaa tttcaaataa attatttttt ggctcattaa
     3121 tatttgcagg agttgtagtt tcattattat tattcttatg gatgaatgct agaaagaaag
     3181 aaatagcagt attactatca ttaggtatat caaaattaga aatttttggt caattcctaa
     3241 ttgagatggt atttatatca atccccgcat tcgttggttc ctatttctta gctcaatata
     3301 cagctgacaa gcttgggaat aatatattga ataaggttac tggtgatata gctaaacaaa
     3361 tagctaaaca atctgcatct agccaattag gtggtggggc agaagctgaa ggatttaaca
     3421 agacattatc aagtttagat atcaatgtat tacctaaatt catgatttat gtagttatat
     3481 ttatgagttt agtacttctt gtatcattga ttatttcttc attcaatata ttaagaagga
     3541 atccaaaaga attattaatt gacaataatt aaaattttcg gtgcttttta gcaccaaaaa
     3601 tgagagtgta atataaaccg tgtaagtaac catcaatcct aggcgggtgg ttgcttcacg
     3661 gtttttattt ttctaaaata agatcaagag gtgattacac gatggctaga aagaaaagaa
     3721 atcctgatgc tgagaagtta gctgaatcca ttttgaatac ttatcaaccc gaatctgtag
     3781 aggatatgca agatgtttta aaagatgtgt ttggtccctt atttgaaaaa atgcttcaag
     3841 gagaattgaa taaccattta gggtatgacg cccattctaa agagccaaag aaacatgaca
     3901 atcgacgaaa cggttatggc aataagacgc ttaaaacgag ctttggtaaa gtaaacattg
     3961 atgttcccag agaccgggag gcttcttttg aacctgagtt gattcctaat agaaaacgag
     4021 atgtctccga catc
//
LOCUS       DAMEET010000137         3979 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.143, whole genome shotgun sequence.
ACCESSION   DAMEET010000137 DAMEET010000000
VERSION     DAMEET010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.143"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(573..1130)
                     /locus_tag="PB780_002494"
     CDS             complement(573..1130)
                     /locus_tag="PB780_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734506.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="PB780_002495"
     CDS             complement(1309..1920)
                     /locus_tag="PB780_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734507.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="PB780_002496"
     CDS             complement(2033..2290)
                     /locus_tag="PB780_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734508.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="PB780_002497"
     CDS             complement(2887..3165)
                     /locus_tag="PB780_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734509.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="PB780_002498"
     CDS             complement(3199..3450)
                     /locus_tag="PB780_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734510.1"
                     /translation="MKEKPVNKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1215 a          810 c          528 g         1426 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgctgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttacaggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgtatccgcc gtttgtataa ttaagcgaga caaataaaaa
     3961 agccatttat gttacactc
//
LOCUS       DAMEET010000138         3978 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.144, whole genome shotgun sequence.
ACCESSION   DAMEET010000138 DAMEET010000000
VERSION     DAMEET010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.144"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(361..582)
                     /locus_tag="PB780_002499"
     CDS             complement(361..582)
                     /locus_tag="PB780_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734511.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(680..925)
                     /locus_tag="PB780_002500"
     CDS             complement(680..925)
                     /locus_tag="PB780_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734512.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1165..1779
                     /locus_tag="PB780_002501"
                     /pseudo
     CDS             1165..1779
                     /locus_tag="PB780_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2149..3150)
                     /locus_tag="PB780_002502"
     CDS             complement(2149..3150)
                     /locus_tag="PB780_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HDE0734513.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3309..3575)
                     /locus_tag="PB780_002503"
     CDS             complement(3309..3575)
                     /locus_tag="PB780_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDE0734514.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3565..3921)
                     /locus_tag="PB780_002504"
     CDS             complement(3565..3921)
                     /locus_tag="PB780_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDE0734515.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1251 a          719 c          526 g         1482 t
ORIGIN      
        1 gacgtctttt ttgaattgtt tgcctttaaa atgattcatt actctgtcct ctctgtcttt
       61 tttctcaatt ttacactaaa atagattttt tggaaaactt tgcaacagaa ccgtttttac
      121 tatatacttc ttcccagaac ttgtccgtct ccttcaaatt atctctacct ctcgagttca
      181 ttgggtggtt tgcccattta ttaaacatgg aatcaccttt ctcatactca tatatttatg
      241 tcctatttca attatattta tatatgctta aataaatttg tatcctgaaa tttattgtaa
      301 tatgaagctg tattttacat atagtaacca aagagttaat aaaataaaaa gagcgaacca
      361 ttatagatca gctctttttt cattgctgtt tttaatctta tctagaatca cttcaaatgt
      421 ttcaaaggta tttacatcta actgacataa ccaaagcaag tacttctttt ctttgtaagt
      481 ccaatcaata ttacggtaag aggacattag attatggtac cttgctcttt gttcaactga
      541 agccaattcc cagcgttttt caaatatttt ttcttcttcc attattgatc cctcgcaaga
      601 cgctacctca aacttaaata atgggcagcg aaagtctaat tccttagatc taagtatagc
      661 aaacaaatat gaaaaaaact tagaaggtag taaaagttct tccgtctttt gctttgcttc
      721 tattcttaat gtgtttcgtt atcggtgccc tacaaagatt ttcattaatt acacactcat
      781 ttattgttgt gtattttctg acatttctct cttgatcaat tattttaatt ccatgggcat
      841 cctgatagtt cataggagag gagcggctat cagaaatagt tgcacctatc tcttgaactt
      901 cttttacaac ttctggaaaa tccatccatc ctgcttcagc tgtcaaaagt aataatctat
      961 aatatttatc tttccttttc aatctaagaa atcgtcaccc cattcttttt cttctattat
     1021 aaactattta acttttagaa ttcaaaaacc gtttgtctat ttagagttta ccctaaatag
     1081 acaaaaatat aacattgaaa aatttagagt tctaacgtat gattagggtg tattataaac
     1141 gttatagaaa agtagggaat aaatttgaaa attgcttata ttagagtttc atccatcgat
     1201 caaaatgaac aacgtcaaat tgaagaaatg aaaaagtttg gcgctgaaaa aatttttatc
     1261 gaaaaacaat ctggagcgac gattagtcat cggccgattt tccaggaagc tttagatttt
     1321 gtcagagagc aagatatttt tattgtagaa gccattgatc gacttggacg caattatgac
     1381 gagattatcg attcagtgaa ttatctaaag aagaagaatg tgcgacttat tatcaccagt
     1441 ttaccgatca tggctgaagc aattggaaat cctctcctag ataaattcat caaagactta
     1501 atcatttaaa tctcagccat gattgctgaa caagaacgta ctgaatcgaa acgacgccag
     1561 gcacaaggaa ttaaaatcgc taaagcaaac ggcgtctata aaggtcgtcc taagttatac
     1621 agtgcagaca ccaaagatcc tcaacgtcgc cttgtctata ggagtatcgt tcaggatctt
     1681 gagaatgggg ttgctatctc aaagattgca acagactaca atgtgactag gcaaacaatt
     1741 taccgaataa aaaaagaaat cgaccaacta attgtatgat ttcttttttg aatcacgtaa
     1801 ttattaatta gctttgtcat caatacagac aatcatttta gattcatcta gctaagttga
     1861 ttacatttat gttaaaaaga atttagttaa cataaatata attgatgcat aaatcgtcct
     1921 tatttaaagg catttaacat tgacaactta gtataatgta tcttatgtaa actaataaga
     1981 tttgaatttt attttataca cactcgataa tagatcaatt ttatggtccc ccgcatttac
     2041 gattgcacaa gagaaacata aagtcgacct attagcattt cttttcaatt caaacattaa
     2101 aaaagtttat acctctgcta ctaaaaaatg aaattatcta tttaaaattt aaaacggtaa
     2161 atctgattca tccaaattgg aaaatggatc agcctgtatt ggagctttgt atttctccat
     2221 aataacagat atcttttttt cattttttaa aaaatacctt ttaaatttct tatagtcctc
     2281 tgaaatcaaa atatagcgtg atggatactc atcaaaagaa gaatcgtatt cttcctttaa
     2341 aattcccgat ttacttaatt tttctagtac atcaaatact ctatcactca agtaactcct
     2401 taaattgtta tcactttccc attgaactac ttttctataa gttgaattat cagttgaaaa
     2461 gatagaattt tcatccataa acgcatatat tagcagcaat ctttcatcat tgtcaaattc
     2521 tattggatca aaaatatcta agttgtcatc aaaatcttta tcagttccgg taactttttt
     2581 gttaattact gaaggtgtct tgtaagaaat agaatctaaa tcaaagcgtt ttgattctaa
     2641 attattttgt gctttctcaa ttaaataatt cacttttttt gtgagaaacc ttctgatatt
     2701 gtctttgcta tatgttccat aaaaaccctt atcttctaaa gagtccctgt acttcttaac
     2761 ttctatatac tcttcagcca aaacttgtgg aactggttca tctaagaaaa aaacccctac
     2821 ctcgcctttg ctatttttat aaaagtaatc aatttctgct atagttcccg atctataatt
     2881 atcaactgga gaacccaact tatggccaaa aatcgctaat aatatatcac tattttctac
     2941 aatttgttta ttaataacac tttgcccaga gctattattt atataagtag gaatcgcatt
     3001 cgtttcccat cgaattggca tcaaaattac ttttgtatta tctgtatttt ctatattcca
     3061 tctatatatg gtttcttcta cttcattcct ctttgctgct atatcacctg gtgatgctat
     3121 aaatactttt attagattta tggttttcat ttattgtacc tttctcttta tgtctcttta
     3181 aagtaatttt tgtataaaat ttattgactt ttacactata ggacaacttt tatctattta
     3241 gtacaaaaag gcccactaaa ctaattgatt agttaggagc ctattacagg tatattttca
     3301 actcgctttt aagcaaattc tactaccagc tttttgccta atgccctagc gatatcatca
     3361 agtttatcga aagatacgtt atcccctctc tcaattcgag caatagtaga ttgagttgta
     3421 tgtgcttttt gtgcaagctc cgcttgagtt aggcctgctt cctcccgtgc atgataaaga
     3481 gcaaccgcag tttcgagttt tcttccttca cggtcatatg cttctgcaaa atctttgtct
     3541 ttttgctttt cttcaatcaa ttttttaatc atcatttgtg aaccactcct ctctaagttc
     3601 ttttgcatgt ttaagttcac ttattggtgt tttctccgtt tttttagtaa atccatgggt
     3661 aatgacatac cttccattga ccacatggaa atagatagct ctttggatgt ttgatcctac
     3721 cttacttctt aattcgtaca aattagtgtc taattttttc acccatttca accgttgagc
     3781 tactagcatc ccattctttt ctgtttcttc tattgtacgt aaaagcttcg cactatcttt
     3841 ttttggtaat gtttccaacc attctaaaaa ttcatcatga ccattaggtc ttttaaatgt
     3901 ttcaaaagat attttttcca tacccaatta tatcatatat tgattttata ttatagtata
     3961 taacataaaa attcgaat
//
LOCUS       DAMEET010000139         3785 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.145, whole genome shotgun sequence.
ACCESSION   DAMEET010000139 DAMEET010000000
VERSION     DAMEET010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.145"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..171
                     /locus_tag="PB780_002505"
     CDS             <1..171
                     /locus_tag="PB780_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDE0734516.1"
                     /translation="SLFPLYHGSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFK
                     IEYTKKRQRLAY"
     gene            406..642
                     /locus_tag="PB780_002506"
     CDS             406..642
                     /locus_tag="PB780_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734517.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            649..2601
                     /locus_tag="PB780_002507"
     CDS             649..2601
                     /locus_tag="PB780_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HDE0734518.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="PB780_002508"
     CDS             complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="PB780_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HDE0734519.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3400..>3785
                     /locus_tag="PB780_002509"
     CDS             3400..>3785
                     /locus_tag="PB780_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDE0734520.1"
                     /translation="MKYILSFCTPKLNYLLAYIRLYSGVLHLRDSVRVSEKEKIKVTE
                     MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRKKIENPHPLLQTTVE
                     PSKPEQREMLLDALLEISDSDPLLRY"
BASE COUNT         1344 a          614 c          655 g         1172 t
ORIGIN      
        1 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
       61 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
      121 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatta agtagttcag
      181 ttttggagta caaaaagtga aaatgtaaac ctaaatgagt atcaaattgc aactgaattt
      241 gatactcgtt ttttattgtt aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag
      301 ttaaaatgta tataaaaatg cgaacgtgaa ataattattg taggaatgca aattggtgtg
      361 ttaaaatggt tacaatttac taagcaaaaa ggggtgcatt ttcctatgca atttcttgag
      421 ccaaaaaata agaacgctaa gtctgtggat tgggaaatat cagagcaagt aagggtgata
      481 gttaagcaat acgctgaata tgcagagcgt actgaaagcg aagcagtgga tgaattctta
      541 ttgaacattt tagacgataa gaagtttatt gaatggattg ccaacaagcg aagtaataag
      601 cggattgttg aaaaaatggg tatcaaagat agagtgggtt gatagacaat gaccaagctt
      661 aaaagattag ccacgaagga agatgaaatt gtagatgtta aaattccgat ttcaaacgaa
      721 gaattaaaag atagggctaa acaatatgat ttattaactc caaaacgatt tgcaacaagg
      781 tataacaaaa tgttgttctt gccaacgtct tttaaatgga acggttctga atatcccatt
      841 caatataatt actgcatcaa tccgttttgt tgtaattttg ggaaagaaca gcacaaattc
      901 aaagatgtaa aagggaaacc aagtcgatat aaaatgactg gtagttccaa agataaaggt
      961 cataaaggta tgtactgcaa tgacaatcct attggcagag gtgtatcgca aaactgtaca
     1021 gtaacccctt tatctaattg gtctgttgtt gaagaaatta aacgccttat agaaatcaat
     1081 agcatccaag atgttgagcc agattatcaa tttcataaag aaggctgctc ggaagaagaa
     1141 tcaaccccat tcaatgagcc gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct
     1201 caacgctatc aatgtaaggc gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa
     1261 accactactt atcaccaaca aaagaacacc attcttccaa tgttcgctaa gatggttgtt
     1321 ggaagggttt cagtcagtcg tacttgtgat attttaggaa ttggagttgg aacctactat
     1381 cataagctgg agtggctgta taggcgttgt ttggagtttt tagaacgata tgagacacaa
     1441 ccccttcaaa caaagaaatt taacgagatg tggcttaata cggacaagat gcactactac
     1501 ctaaataacg tccgtaagaa aggacaaggt tcaaagaaat atacggggtt tgaagacctt
     1561 aatatgcaaa cgtatatagt tgtatctgct gaggtcttat caagatatgt gttccgttct
     1621 gatgttgcct atgattggaa tatttcaatg gatgaattaa atgaagatac aagaaaattc
     1681 aaagaagacc atctcaatac ttttagtcgt aaaaatgata ggttagattg gtcttattat
     1741 ccacaagaac catccgctaa cgatagcgaa aataggaatg cttatcttca cgaattaggg
     1801 aaaattacta ataggagtag atttgtcgat ggtttaaatg ttgatgctcc ttatactaca
     1861 actgctcact attggttaat taaacaaatg gttaatgccg acgaatggcg aatgattagt
     1921 gatgatgact tctctattag aaacgccttt tatagagtat tcacgaaaga attaaggttg
     1981 agcgatgcac atcattttat ttgccaagtt aacaagacga aaagcagaaa acaatgctta
     2041 aaagaattcg gacaagcaaa agcagaactg ctcgattggg gagatatcag aggattcaaa
     2101 acaaagtttc tacggacttt ggcatctcat tatctaactg aattactaac aagtcatcaa
     2161 ttccacgaag aggctattag taaagatgga gaaagatatc gcaaatatgc cgataaccct
     2221 atcaaacatc cattagccac taaggataaa ggattttatt cagttgattg tagaacggat
     2281 ttatctgctc tggaaccaaa cgaaatcgca aagatgctat taaatgttaa tgaccattcg
     2341 accaatagct ttattcaaca gataagacgt tatatatcat ctcttgaaag accattaaca
     2401 acagctcgtg gtgataagaa gagctatatt tacgctaact tcaatccgaa gtatgctcaa
     2461 tttgcgatta caattctaag aacttactac aacttttgtc gcccttttaa atcagcggat
     2521 aaaaaggttt tgacacctgc tcaaagatta gggattacag ataaacaatt tgattggaaa
     2581 gatataattt atttcaaatg acaaaaagag cttggagtaa tcccaagctc ttgtgcttta
     2641 tctttactgt tcttcttcaa ttaacgcacc cgttaactca ataagttttt tctttcatat
     2701 acatgaaaat agtagttata cggattttta tcattcttta tccctttttg ggcaaatacc
     2761 tcattccatt cctcataatt cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt
     2821 atttttgtga tgtacatttt ctcaacataa gggaaaaaca aattataaat ctgttctcct
     2881 ccgaaaataa aaatttcttc ttcgttttta cataactcaa aaacatcttc tattgaatga
     2941 acaatttcac aaccattaaa ggtaaacccc ttatctctcg tcagaataat atttcttctg
     3001 tcaggtaagg ctcttccgat tgattcaagg ttcttcctac ctaatattat cggatgtccc
     3061 tttgtagtat ttttaacata ttcccagtcc ttgggaatcc tccaaggaat gtcattctct
     3121 ttgccaatca ctctattctt atccatcgca gcaatcaaag aaactttcat ttataacaca
     3181 gcctttttaa ctaatttacc atctaatttc ttatttctat caaaaaacct tttctcatta
     3241 tcctgcccgt tagttgaata agcaattttt tctataagtt atattatcat aataccacaa
     3301 aatcacaaat tttcattcaa ttatcatttt caattgcatt ttataaatca ttttgtatgt
     3361 aatttggtat gtaagttaaa atgaaaaaag tagtccattt tgaagtacat tttgtcattt
     3421 tgtactccaa aactgaacta cttacttgca tatatacgcc tttatagtgg agtactacat
     3481 ttacgagatt cggttagagt atcagaaaaa gaaaaaataa aagttacaga aatgtatact
     3541 tcaataaatg gtgaattatg taagattgat agagcttatt ctggagaaat tgttattttg
     3601 caaaatgagt ttttgaagtt aaatagtgtt cttggagata caaaactatt gccacagaga
     3661 aaaaagattg aaaatccgca ccctctacta caaacaactg ttgaaccgag taaacctgaa
     3721 cagagagaaa tgttgcttga tgcccttttg gaaatctcag atagtgatcc gcttctacga
     3781 tatta
//
LOCUS       DAMEET010000140         3735 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.146, whole genome shotgun sequence.
ACCESSION   DAMEET010000140 DAMEET010000000
VERSION     DAMEET010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.146"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(482..907)
                     /locus_tag="PB780_002510"
     CDS             complement(482..907)
                     /locus_tag="PB780_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734521.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            1281..2252
                     /locus_tag="PB780_002511"
     CDS             1281..2252
                     /locus_tag="PB780_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDE0734522.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            2284..3681
                     /locus_tag="PB780_002512"
     CDS             2284..3681
                     /locus_tag="PB780_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDE0734523.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
BASE COUNT         1291 a          604 c          773 g         1067 t
ORIGIN      
        1 aaaaccaaac gacgggaaag aaaaattact acgtgatgta gaataagcct ttctatcgtt
       61 tggttatttt atattcaaat atttgaaata aataaatctt tactctttga tttgtaaact
      121 gaaatgatcc actgtttgtt ttactgatga agcttgatca aaggaagaag ctgagtttga
      181 agttcataaa aatcagccat agtgagtttt gcttggattt gtttatcttc ttctactttt
      241 tacataattt tgaacaggat aagattcaaa tgttttaaat gaatctttga ctagtttgtg
      301 acagttgtcg catatgctca agcttccttg gtcattaaat tctgtatgga tatttttgtt
      361 gttattacat gtacaataat caggactatc gatcatccat ttcgtctcct ttacaatcat
      421 cttgcttctt tactatagca gaaaagaagg ggagaaacaa aagaagatcc aacagacggc
      481 actatagact gtagatgttg ccatcctcat ccttcaaacg gtaattaccg atttttgtaa
      541 atatgactgt tgtttctttt ttattttctt ttcctgaaat aatatattcg tcatctttgg
      601 tagtgatctt gatatctgtc agcactgttt tttcttcaga tgattcagaa gaatcagttc
      661 cttgtgtctg atacgtgcgt gtccattcca tttttttatc tgtaaatgtc aactgtcccg
      721 cggataaatc catatcttta cgtactttta gcgtatatac actgttggtg gtatctgctt
      781 tttccgcttt taagacagaa gaatcggttg tttcgtccga ggattggtca gagccgccaa
      841 acaaatcaca gccagtcaat gtcaaaagtc ccgcagacaa aataaatagt acgattaatt
      901 tcttcaaaat acttccccca tttgatagga taaagaaatt ataccattgg aatgtaaaac
      961 ttcaaacgga tggaagagaa aaacgcacaa aacacccaca aatagagtaa attttagaat
     1021 aaaattttaa aaaggcaaaa aatccgccac tttcagaaag cgttttaaaa aagtgttgca
     1081 atcttttaaa aaaatgctat attaaaaatg aaccggttca aatacttgtt taagtataat
     1141 taatagtgag aggaagttgc tctactgaag actttagtta tatggatatt gatgacaagc
     1201 aacactatgc aaagaatgcg gtcatcataa tcgaacaaca agaaagagaa atcaaatgta
     1261 actggcagga gtgagaatgt atggcatcga tccgtgatgt ggctaagatg gctggtgttt
     1321 cagtgggaac agtttcccgt tacttgaatg gtcagcaact gaaggaaaag aacatgaaaa
     1381 aaatagcaga agcgatttca gcgttggact ataaagaaaa catcattgct aaagggttga
     1441 agaataatcg aagcttctct attggacttt tgataaacgg gatttccagc cgttttgggt
     1501 cggaagttgt ctcagggatc gaaagagtag ctgaagaaaa tgggtatagc ttattattaa
     1561 gcgggtttgc cgatcagcca gaaaaaattg aacaaaaaat tgaatacctg atgaagcatg
     1621 tgatcgatgg gctgattgtc ttcttatccg aagaagagtg gtctggattt gaaatgctca
     1681 caaaaatgag tattccagtt atcgcattga attgtccaga aggaccagca ggtattgata
     1741 caatattagt gaatgatcga gaaagtgtag caaaagtgat cgctcatcat ggtgaacaag
     1801 gacataaaaa aatcggattt attgctgcca ctcaaacaga ctacgtagca agagaacgtt
     1861 tagcaggagc caaagaagcg gtccaaaatg atccatcgat ccaattagag gtctttacag
     1921 gagactattc tagaaaaagc ggctattacg gggcaaaagc attattagaa aaaggggcaa
     1981 ctgctatttt tgtcagcaat tacaatatgt cgattggtgc catcgaatgt ttcaatcaag
     2041 aaggtgtcag aatcgggaag gatattgcgt tcagtcacta tgattattta gataaaaacg
     2101 atctgagtat ccttccgaaa atcacgatca cgccaccaac agataaaatc ggggaacgag
     2161 cagcagaaca gctgttaaaa cgaatcaaaa aagaagagga agaaaatgaa aaagtgattg
     2221 tgatggaaaa tatcatacat ggaatcaact aaaacgaact aaaaaagaaa aagaggtgct
     2281 ttgatgaaaa aatatcaatt tccagcaaat ttttggtggg gttctgcagc aagtggccca
     2341 caaacagaag gaaggatttc aggcgatgga aaaggagaga acatttttga ttattggtac
     2401 aaaaaagaac cgcaaaagtt ctttgatcaa gtaggacctg aaaaaacctc gcaagtttat
     2461 acgaagtatc aagaggatgt acagttgatg aaacagactg ggcataattc atttcgaaca
     2521 tctatccaat ggagccgttt gatacctgat gaatctggaa caatcaacga agaagccgtt
     2581 cgtttctatc atcgttattt tgatgagttg attgaaaacg gtatcgaacc ctttgtgaat
     2641 ctgtatcatt tcgatatgcc gatgtatctg caagaaaaag gcggatggtt gaaccgagaa
     2701 acggttgaag cctatgaaaa atatgccaaa agttgttttg aattgtttgg ttcgaaagta
     2761 aaaaaatggt ttactcataa tgaaccaatc gttccagtcg aagcaggtta tttatatcga
     2821 tggcattatc cagaagagtc agatatgaaa aaagcgatcc aagttggcta tcatgaagcg
     2881 ttggcttcag cgttagccat caaatcttac catgaaatgg agcaaggtgg tgttattggt
     2941 atcatcttga atctgacacc aagctatcca agagacgaat caaaccctga agatgttaaa
     3001 gcagcgcaaa tagcagatgc gttcttcaat cgttcattcc ttgatccagc tgtaaaaggc
     3061 gagtttccaa gtgaattgat tgagatagta aaagaacttg atctcatgcc tgtgatccat
     3121 gcagaggatt tgacggttat caaagaaaat acagtagatc ttttaggtat caattattac
     3181 cagccacgcc gggtaaaagc aaaagaaaca ccaatcgata ctaaaaacgg tccaatgcct
     3241 gaggattatt tcgacaatta tgaaatgcct ggtcgtaaaa tgaatcctta tcgcggatgg
     3301 gagatctatg aacaaggaat ctatgatatt ttgatcaatg tacgagatca ttatggaaat
     3361 atcgactgct ttatttctga aaacggtatg ggtgtagaaa acgaagcacg atttatcaaa
     3421 gaagatggca tgatccacga tgattatcgg atcgaatttg tccaatctca tctgaaatat
     3481 gtccatcaag ccattcaaga aggtgccaat tgtttaggct atcatatgtg gacatgtatg
     3541 gataactggt cgtggaccaa tgcatataag aatcgatatg gctttatttc agtagactta
     3601 gcacaagatg gaaaaagaac aatcaaaaaa tcgggtcatt ggttcaaaga cgtatcagat
     3661 aaaaacggat ttgaggctta agaaagaata gttgagagca aatgcgtgaa attctccatt
     3721 cttaccattt tgtga
//
LOCUS       DAMEET010000141         3663 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.147, whole genome shotgun sequence.
ACCESSION   DAMEET010000141 DAMEET010000000
VERSION     DAMEET010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.147"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..276
                     /locus_tag="PB780_002513"
     CDS             <1..276
                     /locus_tag="PB780_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HDE0734524.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            419..640
                     /locus_tag="PB780_002514"
     CDS             419..640
                     /locus_tag="PB780_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDE0734525.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            656..1498
                     /locus_tag="PB780_002515"
     CDS             656..1498
                     /locus_tag="PB780_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734526.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1561..3363
                     /locus_tag="PB780_002516"
     CDS             1561..3363
                     /locus_tag="PB780_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HDE0734527.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(3422..>3663)
                     /locus_tag="PB780_002517"
     CDS             complement(3422..>3663)
                     /locus_tag="PB780_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDE0734528.1"
                     /translation="PPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQNFNSRSV
                     KGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1306 a          519 c          746 g         1092 t
ORIGIN      
        1 gaactcatca agctggctaa taagggtcaa gattatgaac atctagcaga tgagattgat
       61 gagctgagag acaagcgaca gctcctttta gtagaagatg cctccctcag tggcgagaac
      121 gagcgaatca atgagctgat tgaatttatc cgcaagaaca aattccgtac cttagagtac
      181 gatgataagc ttgtaaggaa gataatccag agcgttacag tctatgaaga acacttcgtc
      241 atatccttca aatctggcat cgaaatggaa atatgaaaac cagaagtaaa tagcccatga
      301 ttctacagta gagtcgtggg ttttcttttg cttgctgatt ataataatat tttaataact
      361 attgttatta taaaccaaat gatttataat attcttagcg taaacttgga ggtgctgtat
      421 gactacggca gaaatgatta aagaactgtg tgagcaaatg aatataagtg tttccgaact
      481 tgctagacgt attggacaga ctccacagaa tttcaataag aaattacaac gagaaacggt
      541 aaccttagat gagttgaaag cgattgctga tgtgctaggt gtcaagtttg tgcaggcttt
      601 tatattgcct gatggcaatg aaataaagat aggtaattag gaggtgtaat ttgtattgaa
      661 aatcataaca ataaatgatg ttgagtatgc tgtatttgct gctaatgaag gtacttccaa
      721 accacaacct catatcatag aaacgaaatc tggtactatt ccagaaggaa aacaactgag
      781 tttattaaaa gaatatctta agcagaatga tatttcccca ataaaaggag caacaacata
      841 ttggtgcatt gacaaggttc ttaagcttga ttcaagtaaa gaaaaaacta taagtgaaac
      901 aatccataaa caaaaatact tatctttaac tgaagaaaat attgagaaac agcataagtt
      961 tgtaggtgca agctctaatt atgggaaaga aggtctaata atacatgatg ttttgaatgc
     1021 atttcctctt cataatgatt taaatactat tgcgatgaag attgcggtaa ttgatgtaac
     1081 aaattcaaca catctttctc aatataagag caggttgtct ctatatgatt tggctaaagt
     1141 aattctggag attccaaatt ttgatgatcg gttggcaaaa ggagatcctc agttgataaa
     1201 tattatcgca aggaacattg gtgcagttaa tatgttctca tttgcatcca agtactgtac
     1261 atatcataat gtggaagtgt gtgggagaga tgattactcc atctttgatg gtatcgttaa
     1321 aaatactctt ccacactata tccaaggact aactacgaat aagattgata cgtggcgaag
     1381 aagttttgat tacgaggcat ttaacgaatg tgttggaaaa ctacttgatg aaaataatat
     1441 acatattcct tttagaagaa gaaagttaga tcacttcttg tggtatgcga ataggtagaa
     1501 tataagaaaa aggcatcgta aaagtgattc tggataaaat taatggggga ggaaataata
     1561 gtgggattat ttgattttct aaagaaaaaa caaggaaagg ttagtaaaca cgttgattat
     1621 caactggctt ctagtctatt tcgtactggt aaagtttttg ctgaaaaatt ggaagtggac
     1681 atatcaaatg tagatgagtt tcgtaaaact tttatcgctt ttgatgtgga aacaactggc
     1741 ctaaattctt cttcagatag aattgtagag attggtgcgg tgctatttaa agaaggagtt
     1801 ccaattcaat cattcggaac tcttgtcaat ccaaaagtta gaattgcagc ctcagcttcg
     1861 gcaataaata atataactaa tgaaatgcta aactctgctc caaatgagca ggaagtttac
     1921 ccccagttgg tagaattttt aggagatgca ctagatggaa aaatcactat gtgtgcacac
     1981 aatgcaagtt tcgattttgg atttttaagt aacacatttt ctagacttgg gtatgatgct
     2041 aaaattaagt atgttgatac tctgagtctc tctagaaaac atataaaagg cattgtaaat
     2101 cataagcaag agactgtagc ggaatatttc ggattaataa atgacactgc ccatagagcg
     2161 gaatcagatg cagatatatg cggaaagatt ttttggggtg ttttagatat tattgaggag
     2221 tcgttggaag aacaaaaaaa gcaaatagaa aagatgactc cagacataaa agaacttgaa
     2281 gcttgtgctt ttattcaaga tattattgag aaaaaaggat gcgacaccag ttggatcagg
     2341 tatcgaaaaa acagtaataa ttatgtcgat gtaacttgtc tatatacatt cttaaagttt
     2401 aagtttgcta agaagggaaa ttacatcatt attagaaaag atgctacaaa tggaattgat
     2461 ttacctatag aaccttgtac tgtaagtgaa ggcggtacaa caaatgtgcg agtctatttt
     2521 cgtagcccct ttgacttaac gcctctttca aattatattt tcagtgctta tagcgattgt
     2581 tataattcta tgaatgaata tatgagtgtt agtaattaca ctaaaaaaga agcagagcaa
     2641 agccttagta tgctgaaatc tatttcaaaa tcagcgatga aagagttatt attcgatgca
     2701 aaaaatcgtg gatatgatga gactgaagtt aacctaaata ttgaaaaagt gatatctaga
     2761 gaagatgtaa ttattaacgg gttgcataat cgagtttcat taaacaagat acgaaatata
     2821 gggaattggg agaaaggatt tgatgcaggt tatccatatt gggaacgtgg agaaactgct
     2881 aggaaaaatg gcaaagtaga tgaagctatt gcactttttg ataaagctcg atataacggc
     2941 tatgatgctc ctgcgctata tgattcttat gctaaagctt atcgaaaaat taaggattac
     3001 gataatgaaa ttctcattct tgatgaagga attatgcgta aaacacggca cgatgttggt
     3061 actttagagg ctcgtaggga taaggcaatt aagctattat ttgctaaaca agaggcagag
     3121 cggatagcta aggaaaaatc tgattttcta aaagcgaaca aaaaagagga tgcgattagt
     3181 gagcctagca agaagcgtgg aagacagatt attcaaatga ctgatgatgg aacaataata
     3241 aaagaattcg atactatagc ttctgctgta aaagaagttg gcgttaactc aaaaagtatt
     3301 agggatgctg caaatggagt tcaaaaacat gctggaggat actactggaa atacaaagat
     3361 tagcagataa tctaagataa cctaggatct tcttaataga tacccgtggt gctagttaat
     3421 tttaatatcc taaagaatat ctcaaagttc cttcaaaacg tcgttgtgct cgacttaata
     3481 gaacactgta agccagtaat atgctttcaa atctactctc tagcccttta acagaacgtg
     3541 agttgaaatt ttgacaacct aatttttcta aggaagaaaa aacagtccca actgtttttc
     3601 tttgttttcc aagtaagctg tggtctagtt tttctgattt atccatgttt ttacgtggcg
     3661 gaa
//
LOCUS       DAMEET010000142         3601 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.148, whole genome shotgun sequence.
ACCESSION   DAMEET010000142 DAMEET010000000
VERSION     DAMEET010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.148"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..185
                     /locus_tag="PB780_002518"
     CDS             <1..185
                     /locus_tag="PB780_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDE0734529.1"
                     /translation="EDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVY
                     EGHFVIAFKSGIEMEI"
     gene            328..552
                     /locus_tag="PB780_002519"
     CDS             328..552
                     /locus_tag="PB780_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDE0734530.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            567..1436
                     /locus_tag="PB780_002520"
     CDS             567..1436
                     /locus_tag="PB780_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDE0734531.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1417..2151
                     /locus_tag="PB780_002521"
     CDS             1417..2151
                     /locus_tag="PB780_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDE0734532.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            2184..3092
                     /locus_tag="PB780_002522"
     CDS             2184..3092
                     /locus_tag="PB780_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HDE0734533.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            3089..3569
                     /locus_tag="PB780_002523"
                     /pseudo
     CDS             3089..3569
                     /locus_tag="PB780_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1251 a          521 c          788 g         1041 t
ORIGIN      
        1 tagaagatgc ctccctcagt ggcgagaacg agcgaatcaa tgagctgatt gaatttatcc
       61 gcaagaacaa attccgcacc ttagaatatg atgataagct tgtaaggaag ataatccaga
      121 gcgttacagt ctatgaaggc cacttcgtca tagccttcaa atctggcatc gaaatggaaa
      181 tatgaaaacc agaagtaaat agcccatgac tctacagtag agttgtgggt tttcttttgc
      241 ttgctgatta taataatatt ttaataacta ttgtgtttat caaccaaatg gtttataata
      301 ttcttagcgt aaacttggag gtgctgtatg actacggcag aaatgattaa agaactgtgt
      361 gagcaaatga atataagtgt ttccgaactt gctagacgta ttggccagac tccacagaat
      421 ttcaataaaa aattacaacg agaaacagta accttggatg agttgaaggc cattgctgat
      481 gtgctaggtg tcaagtttgt gcaggcattt attttaccag atggagaaga aataaaaata
      541 tctaacgaat aaaggaggat gtttatatgg taggtaatgt tattcaaata gtaacagaga
      601 aattatcatc tttgcctttt atagaaggca tcgtattagg gggctcacgt gcaagaggca
      661 cccatacaga aaattctgat atagatatcg gaatctatta caattcagac tcatttgacc
      721 tgacagcgat taatcaaatt gctacagaat tggatgataa aaatagaaac aaccttgttg
      781 tgcctcccgg agcgtggggt gattggatta atggcggcgg atggttattt ataaatgggt
      841 atcatgttga cttgatttta cgtgatataa aacgggtaga acaaataatc aaagatacgg
      901 agcaaggaat tgttactgcc aattatcaga ccgggcatcc ccatggttat ataagtgcaa
      961 tgtatcgtgg agaattagcg attagtaaaa tactatatgc taagaacgaa agcttatgcg
     1021 aattaaaaaa gcaggcagaa atttacccta ctgctctaaa gaaaagcttg atgaactttt
     1081 ttatatttga agctgaattc tctttaatgt ttgtaaaagc aaatgcgggg gtagaggata
     1141 aatattatat tgcaggccat gtttttcgta ttatttcatg cttaaatcaa gtactatttg
     1201 catgtaataa tgcttattgc attaacgaaa agaaagctat aaaactgctt gaaacttttg
     1261 aatataaacc tgaaaaatat gccgaaagag ttaatcatat ttttgaagta ctcggtcttt
     1321 ctctttttga atgctacgat atgaccgaga aactttataa agaagtgaat gaaattgtat
     1381 cggagataaa taacttttta aacgaggaga gttcagatga aagaaaacaa atatgatgat
     1441 aatatatttt ttcaaaaata cagtcaaatg agtcgctcgc agaaaggact ggctggtgcg
     1501 ggagaatggg agactttgaa aaagatgcta cctgatttta agggtaagcg tgtgcttgat
     1561 ttaggatgcg gctatggatg gcactgtata tatgcgatgg aaaacggtgc ttcctctgta
     1621 gtaggtgttg atatttctca taaaatgctc gaagtagcaa aaggaaaaac ccattttcca
     1681 cagattgaat atgaatgctg tgccatagaa gatgtggatt tcccagagga gagctttgat
     1741 gtaatactaa gttcgcttgc gtttcattat gtagcagact atgagaattt aataaaaaag
     1801 atatatagga tgctgaaggc tggtggcaat ttagttttta cagttgaaca tcctgttttt
     1861 actgctcatg gaacacaaga ctggtattat aacgaaaaag gagaaatact gcatttcccg
     1921 gtggacaatt attattatga gggcaaacgg acagctatgt ttttggaaga aaaggttaca
     1981 aaatatcata gaacactgac cacatatcta aatacactgc tttcaaatag ttttataata
     2041 aatcagattg tggagccaca gccgccagag aacatgatgg atattccggg gatggcggat
     2101 gaaatgcgac gcccaatgat gctgattgta tcggcaaaaa agaagatgta ataatataga
     2161 aaaaataaac gaggagtatg taaatgagat cagaaaaaga aatgatggat ttagtacttt
     2221 ctttagcaga acaggatgaa cgtattcgaa ttgtgaccct tgaggggtca cgcgcaaata
     2281 ttaatatacc taaagatgaa tttcaggatt atgatattac atattttgta agtgatatag
     2341 aaccgtttat atctaatgat gactggctta atcaatttgg gaatataata atgatgcaaa
     2401 agccggagga tatggaatta ttcccacctg aagaaaaggg attttcctat cttatgctat
     2461 ttgatgatta caataaaatt gatcttacct tattgccctt ggaagagtta gataattacc
     2521 taaagggcga taaattaata aaggttctaa ttgataaaga ttgtagaatt aaaagggaca
     2581 tagttccgac tgatatagat tatcatgtaa gaaagccaag cgcaagggag tatgatgatt
     2641 gctgcaatga attttggaat gtaacacctt atgttattaa aggattgtgc cgtaaggaaa
     2701 ttttatttgc tattgatcat tttaatcaga ttgttcgcca tgagctgctg agaatgatat
     2761 catggaaggt cggcatcgaa acaggcttta aattaagtgt aggcaagaac tataagttta
     2821 ttgaaaggta tatatccgag gatttgtggg agaaactttt gtccacctac cggatggatt
     2881 cctatgaaaa catatgggaa gcattatttc tatgccatca attgttcagg gcggtatccg
     2941 gtgaggtggc ggaaaggctt cattatgcct atccggagta tgataggaat ataacaaaat
     3001 ataccaggga catgtataaa aaatacactg gtaaaaccgg ctgcctggat agcacatatg
     3061 ccgctgatat agaagagagg cgggaacagt gattacagaa atgaaagcag ggcacctgaa
     3121 agatatcgat aaacccagcg aaccatttga ggtgataggt aagattatac cgaggtatga
     3181 aaacgagaat tggaccttta cagaattact ctatgaagcg ccatatttaa aaagctacca
     3241 agacgaagag gatgaagagg atgaggaggc agattgcctt gaatatattg acaatactga
     3301 taagataata tatcttttat atagaagata tcgccgtatg taaggatttc agggggcaag
     3361 gcataggcag cgcgcttatc aatatatcta tagaatgggc aaagcataaa aacttgcatg
     3421 gactaatgct tgaaacccag gacaataacc ttatagcttg taaattctat cataattgtg
     3481 gtttcaaaat cggctccgtc gatactatgt tatacgccaa ctttgaaaac aactttgaaa
     3541 aagctgtttt ctggtattta aggttttaga atgcaaggaa cagtgaattg gagttcgtct
     3601 t
//
LOCUS       DAMEET010000143         3478 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.149, whole genome shotgun sequence.
ACCESSION   DAMEET010000143 DAMEET010000000
VERSION     DAMEET010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.149"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..2232)
                     /locus_tag="PB780_002524"
     CDS             complement(<1..2232)
                     /locus_tag="PB780_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734534.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="PB780_002525"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="PB780_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          977 a          769 c          549 g         1183 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac gacgaaaaat aaatcactac cagtcaactt ctataata
//
LOCUS       DAMEET010000144         3463 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.150, whole genome shotgun sequence.
ACCESSION   DAMEET010000144 DAMEET010000000
VERSION     DAMEET010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.150"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            137..748
                     /locus_tag="PB780_002526"
     CDS             137..748
                     /locus_tag="PB780_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDE0734535.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            821..1750
                     /locus_tag="PB780_002527"
     CDS             821..1750
                     /locus_tag="PB780_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDE0734536.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1819..2645
                     /locus_tag="PB780_002528"
                     /pseudo
     CDS             1819..2645
                     /locus_tag="PB780_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033645.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            complement(2693..>3463)
                     /locus_tag="PB780_002529"
     CDS             complement(2693..>3463)
                     /locus_tag="PB780_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDE0734537.1"
                     /translation="MTKSFTEEVTSFKERELHDRYAAIYMDATYIPLKRKTVAKEAIH
                     IAVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERGVKNVLLFITDGLKGMAGAVQ
                     RFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMVYQASSKKAALEARGAFAEKW
                     KTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIESFNKQIKKYSHRKEQFQNEE
                     SLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE"
BASE COUNT         1149 a          538 c          660 g         1116 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaaatgt attataggca
       61 tttttttaca agaaatagaa gcttgaagtt tatatgaagt tataaaattt aatttatatt
      121 ggaaggagga aaataaatgt attttatact acctacactt tatagccttt tatgtgtaat
      181 ggcaccatgt attatttata tggtattttt ttgcaaaaaa aataccctga aacacaatat
      241 cctgattttg atttttatgc tgtactgttg gaaagttttt gaattgactg gtacaggtgg
      301 actaacagat attttttata gacctgatac aggtaggaaa ttttctctag tacagggcag
      361 tgttaatctt gtaccattca gtggactaaa cattagcttt atattaaata tagtaatgtg
      421 tgtgccctta ggattcttac taccaatcat atggaaaagt tataggaaaa tagttcctgt
      481 tttcttggct ggggctctat tttcactaat tattgaagtc tcacaaatta taactacaag
      541 agcaacagat attgatgatt taatagcaaa tacctttggt gcggttatag gctttatact
      601 gtgggctgtt ttaagtagag tggtgtcctt gaaggacaag tcatctacag tagattattt
      661 agaaccttat atttatatat tgattggttt taccggaaaa ttcttactgt attttccttt
      721 ctggtttgct tttgaagtat tatcctgata attccataga aaataatgag tttgagttga
      781 gaaagaatat tcttgtacac aactctggaa aggaggaagt atgaaaagat tcttaaaatg
      841 ggggcttgtt cttttagcgt ctttgattgt gattctaatg tgtacagcgg ttgttatttc
      901 ttatgtgaat cggccgtatg caagagctgt ttatgatagg attaaacgca ttcttgacaa
      961 agatttttat gaccatctat atctaggttc ttcatttgaa ataataaacc ttgatacaat
     1021 ggatattcat agctatatga aaaaagacca agaagaatta cccgcctcat tgagcaaatt
     1081 atttacgatt gattatgcgc ttagtgtcct aagtttagat gaacagatta caattagtcc
     1141 tactgcactt gacatgcaga aagctgactc atctactgca catcttcaag aaggagatat
     1201 tttatctgtt tatgacatta tctgtgctat ggtcttaccc tcaggaaatg atgcagctta
     1261 tagtttggca atttatacag gtggaaaact tgatcctgat gcaaagaatg atgaagaacg
     1321 ctatcaaagc ttcatgaaaa acttagatcg ttatctaagt gagcaaggct ataaacatac
     1381 tcattttact gacgctgctg gctatgactc caatgctaca acatcagcgg aagatttgac
     1441 aaaagtagct gcaaagctac taaagaatga taaatttgca gaaatagttg ccaaggaaac
     1501 ttatagagca aaatcaatta agggaaatgt ttataactgg aatactacga atcaatttat
     1561 gcaacaagat aatagatact ttactgaagg tgtaaaaggt gtaaaaactg gatcgtttga
     1621 tgggagatac aatctatgtg tgtattatac atctagttct gataaccact atttacttgt
     1681 aagttttggt tcagagagtg atagtaggcg ttatgtagat gttcaaaatc tattgcataa
     1741 tctgccttga aatttcgtgc ctttaagcgc gtatctaaag tttctttgaa aagtttgaga
     1801 atgaagaaag tgagcagtcg aaaaacggtt gctcactttt ttgccctaaa atcgaaaaaa
     1861 tataaaaaag tcctttacaa cacgtctcag gaaaaactgc taagtcaatt ctaatatctt
     1921 gtggagcaag acttgcacct tttgatataa gagagcttag agaactaatg agcgaagatg
     1981 aactagaact cgataaaatt ggagatagaa aaactgcctt gtttgtaata atatcagata
     2041 cagatgatac atttaatttt gtagtttcaa taatgtattc tcaattattt aatctactat
     2101 gtgataaggc agatgatgtg tatggtggaa gacttccagt tcatgttagg tgtctacttg
     2161 atgagtttgc aaatatcggt ttgattccta aatttgaaaa actaatagcg acaattcgtt
     2221 caagagaaat atcggcaagt ataatactgc aagcacaatc tcaattaaaa gcaatttata
     2281 aagaccatgc agatacaata gttggtaact gtgactctac actttttcta ggaggaaaag
     2341 aaaaaacaac agtaaaagaa ttatcagaaa ccttaggaaa agaaaccata gacctatata
     2401 acacatcaga aacaagatca aatcaaaaat cttttggtct aaattatcaa aagactggca
     2461 aggaacttat gagtcaagat gaaataactg taatggatgg tggtaagtgt atttaccagc
     2521 taagaggagc aagacctttt ctatctgata aatttgatat tacaaagcat aagaattaca
     2581 aattgttgga agattatgat aagaaaaact tgtttgatgt agaagagtat ttaaaacaaa
     2641 gatagcgtaa aatattttgt gtaaataact cgtccttctg caaaataatg ggttactcag
     2701 taaacattga agctaatgta tccgtcactt gttggaagcc tttatggctt ctatttaaga
     2761 atttttgatt gtatgtatca aagatggata ctaggaaacg ctctagtgat tcttcatttt
     2821 gaaactgctc ttttctgtgg ctgtatttct tgatttgctt attaaaggat tcaatcaagt
     2881 tcgtagagta aatagtcttg cgtatggcta atggaaaatc atagaaagtg agcaggtaat
     2941 cgttcgaaag gatcgattcc accatttttg gataactggt tttccatttt tccgcaaaag
     3001 caccacgtgc ttccaatgcc gcctttttag atgacgcttg atacaccatt ttaaaatcat
     3061 cacagacttc cttacgatca tcgacacgca ctttgtgact gatattacgg gaaacgtgca
     3121 cacagcaatg ttgaaaacgg gctttggggt agaaccgctg cacggcccct gccatcccct
     3181 ttaagccatc cgtgatgaaa aggaggacgt ttttcacgcc acgttcctgg aggtctatta
     3241 aaatctcttc ccaaatcgtg atagattcag ttggtgcaat cgcatagctc aacacttcct
     3301 ttgaaccgtc cgggcgaatg ccaactgcga tatgaatggc ttccttggcg acggttttcc
     3361 gctttaaagg gatataggtt gcgtccatat aaatagcggc ataacggtca tgaagctcac
     3421 gttccttaaa tgaagtgact tcttccgtaa atgatttagt cat
//
LOCUS       DAMEET010000145         3459 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.151, whole genome shotgun sequence.
ACCESSION   DAMEET010000145 DAMEET010000000
VERSION     DAMEET010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.151"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(579..1595)
                     /gene="tsaD"
                     /locus_tag="PB780_002530"
     CDS             complement(579..1595)
                     /gene="tsaD"
                     /locus_tag="PB780_002530"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDE0734538.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(1592..2056)
                     /gene="rimI"
                     /locus_tag="PB780_002531"
     CDS             complement(1592..2056)
                     /gene="rimI"
                     /locus_tag="PB780_002531"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDE0734539.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2063..2605)
                     /gene="rimI"
                     /locus_tag="PB780_002532"
     CDS             complement(2063..2605)
                     /gene="rimI"
                     /locus_tag="PB780_002532"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDE0734540.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(2589..3311)
                     /gene="tsaB"
                     /locus_tag="PB780_002533"
     CDS             complement(2589..3311)
                     /gene="tsaB"
                     /locus_tag="PB780_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDE0734541.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
BASE COUNT          988 a          757 c          594 g         1120 t
ORIGIN      
        1 aatatcaggg tcactgctta ttttcctccc taaaacttct gaaagatccg gcagttttag
       61 atcactggac aagactcaaa gattttctat gtgtgtttca gttatgtaat attcactgtt
      121 tgcaaaaaat actgttagat taactagatg aagcgtcttg tcctcatccg atctagatta
      181 ttcgatattc ctcgacaata aacgattact cagtgagggc aatcagagga acggataatg
      241 ataaataagc tgtaaaaaat agcaaaataa caataatgat taaaagtatg taaaaaatag
      301 ggctttgaca aaaacgtgtc acagccctat ttcgattaaa cggtgtgtac ctgtcaactc
      361 ttcggtcaca agtcataatg ttcataaaca tgaggagctg ttttttgaaa ttgttccttg
      421 tgaatcagag ttaaacgaca ggcttacgaa ctcttgatta aacggcgttc aaaagcttgc
      481 atacgcggta ataagaacag ccaggtcaaa aacatgagaa gccgtttttc ctgggctgtt
      541 cttattactt gatgcagggc agctttttca caacctcttt attcagataa aaccaagctt
      601 ggatcagcat tcaactgata gctagcaaaa tttctttttt gcatttccac atgtgctgct
      661 gctccaatca ttgcagcatt atctccacaa agtcgaagag ggggaatcac tagttctacc
      721 tcagggagtt tagttgccaa agcatcctgc aatccttcac gcaacccttg attggccgct
      781 acacctccag ctataatcag ttgcttgact ggataatttt gacaagctcg aagtgtttta
      841 gtaatcaata catcgatcac acttgcttga aagctagctg ctaaatcatt tttgtctaga
      901 tcttctccac gctgttgtgc attatgcacc aaattgataa atgcactttt taatccgcta
      961 aaactgaaat cataattatc ttcatggatc atcgctcgcg gaaaatggta attatctttt
     1021 ccctgatgag ctagctgatc gatttctttt ccgcttgggt aagaaagacc taatactctt
     1081 ccaactttgt cataagcttc tcctgctgcg tcatctcttg tctccccaat gatttcatat
     1141 gatccatctt cttgcatata gactaattct gtatgtcctc cgctaacaag aagtgccatc
     1201 aatggaaact ggaatggttt aaccagacgg gctgcataga tatgccctgc catatggttt
     1261 acaggaataa gcggcaattg gtgtgcccaa gcaaaagcct tagctgcaga aataccaatc
     1321 agcaacgagc cgacaagtcc tggtccataa gtgacagcga ctgccgataa atcttctgcc
     1381 gacacaccag cttctaccag tgcatcttcc aagcataagg tgatttgttc tacgtgatgg
     1441 cggctagcta cttcaggtac tacgccacca aaacgtttat gactgtttat ttgagaagca
     1501 acgatgttag ataagatctc tgttccattt ctaacgaccg ctacactcgt ttcatcacaa
     1561 cttgattcaa tcgccagaat caattcttca ttcatttcat cgtctccgtt tttaactttc
     1621 tgctcataat gatcgcatct tccgtcggat tgcgataata tgatttccgt ttacctaata
     1681 tacgaaactt ttctgattca taaagttttc ttgctgcttc attagataga cgtacttcta
     1741 aaaaaacatg cagcgaatct tttatttgtt ccgtaatcag taattgtaac aaatgccgtg
     1801 catgcccttt tccttgttct gaaactgcaa cagcaatatt tgtgatctcg acttcatcta
     1861 ttactttcga gtatccaata aagccacaga tctggttatc ttcagctaaa agcagataat
     1921 cagagtgagg ctgttggatg tctgccaaaa actgcatttt cgtccaaggt gaaccatgtg
     1981 gataagcatt ttcgctaatc atccatacgc attctgccaa gtacgcttca tcaaaatctt
     2041 tttttctgca aatcaactaa tctcactcat tttctaaaag ttttatcata tccaacgcat
     2101 cttcttttgt ttcagtgtaa tatctttttt tgatcgttgt tgcttgaaac cccaatttcc
     2161 gataaagtct ttgtgcatct tgattactca tacgcacttc taatgacatc gtctcacagc
     2221 gattcattat agcaaagttc tcgatttcgt caataagcag cgaaccgatt cgtttacgct
     2281 gatatgccgg tcgaacggca atattcgtga tatggcaatc taaaccgaac gctcttgaac
     2341 cgataaaacc aatcattctt ccattatcaa agatacctag atatagatga ggaagcggtg
     2401 acttcaattc tgatataaag gcactttttg tccatggcaa ttcgccatta tagacttctc
     2461 gttcgacatc cagcagctct ttgatatctt ctgtagtcaa ttctcttagt acgtaacgac
     2521 gatcgatttg ttcgactgtt ttttctgtat aaatcatttt atcttgaaaa aagcttctgg
     2581 catacgctct aaattttttc aacataactt tcgtctccag gtgtgtgcgt ctccaaccat
     2641 ttttcttctg cttctactcg ttttaaatag cgaggaagaa aagcttgttt ctctgcaact
     2701 ggttcaacta aagcacctaa ttgagccaca gttacaccac ttggaatatc ccatgctgga
     2761 actgtattaa tcatcgcact aggcaatatt tcctttattt cagagaagaa tttgtgacaa
     2821 tcactcccga cgaaataaat agcttcattc agcttttgta gttgtgtgaa caaatcagaa
     2881 gcagaaatat gtacatcttc aagtacagtt tctaatctat tttctttcca gcggtatgcc
     2941 ccagcgtata cattttttct tcgagcatca aacatgggca caataatatt atccacaccg
     3001 atgcaattag ccgcgatggt tttcagacta gatactccta ctagctcttt atctaatgta
     3061 taagcaatcg tttttgctgt cgttacacct atacgaagcc ctgtataaga accgggacca
     3121 tcagaaaccg cgattcggtc aatgtctttt ggacttattt gaagatctgc aaacaattgg
     3181 tcgattgcag gcatcagtgt tacactgtgg ttgcgtttga tatttgtttg tatttgtcca
     3241 agaatttgtt cgtcttctac cacaccgatc gccaaagttt ggtttgcagt atctattcct
     3301 aatgtaatca tatatttgat cctttctgct atgacattct cgccttccat tgtagcatac
     3361 ttttttattc tgaggatgaa aataccaaag gcaaaactag tacaataata catgattatc
     3421 tgctggcggg caaattttgc cccttcctga aacacaaaa
//
LOCUS       DAMEET010000146         3367 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.152, whole genome shotgun sequence.
ACCESSION   DAMEET010000146 DAMEET010000000
VERSION     DAMEET010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.152"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            238..1629
                     /locus_tag="PB780_002534"
     CDS             238..1629
                     /locus_tag="PB780_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDE0734542.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVAIDNYYENTKKLIDDMDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENISS
                     LAESAFPKEKTYR"
     gene            1667..2497
                     /locus_tag="PB780_002535"
     CDS             1667..2497
                     /locus_tag="PB780_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDE0734543.1"
                     /translation="MENKNISILLSLYDKEKPEYLKEALASIFEQTVMPAEIVLVYDG
                     PINEKLQAVVTYFQQKVPHLFTVVKLEKNQGLGIALATGLEHTKNRLVARMDTDDIME
                     PHRLEKQLEIFQEYPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFN
                     HMTVMFDKNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYAR
                     RGGSKYLLPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN
                     "
     gene            2469..>3367
                     /locus_tag="PB780_002536"
     CDS             2469..>3367
                     /locus_tag="PB780_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDE0734544.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLRRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNYGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNHPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIFYYQNNDSI
                     VYQSFNSKQYDNILQRKILVDEVKKDYPRLINLANARLVDGYLSTGFKITGKNQNQKE
                     KQKYYNQSKREIKSIYSKIRSNN"
BASE COUNT         1196 a          467 c          647 g         1057 t
ORIGIN      
        1 tatatgaata ttgtaacttg tgaataagga attagcagga gaatactagt tcagcataaa
       61 ggaagtgatg ggacttctga gacaactttc tgttatgcag aaatacacat ataaacagac
      121 aataaacgct tttccccgtt tcttttaatt tcctttacaa tttggttatt cactaggttt
      181 ttaaactttg ctatggtaag atgatagagg cattatttta tggttaggag tgtatatatg
      241 aataaaaatg gggagtggaa tgcggcacgc agaatattca ttattcttat ggatgtactt
      301 gtattaaatg tatcgatcta tcttagtttt ttgctgaaat tccagggaga agtaccagtg
      361 agaaacttag aaacctttca gcattcagca atttttattt ccatcatctt tattgcgttg
      421 aatattttgt taggagctta tgtattctat aaccgaatga tcagtgatat tgtcttcatt
      481 actgttattg gacaagtact aatagcattg gggatcatgg tagttacttt tgctggccga
      541 tggtttgcat ttccgcgtgg cgtcattttg atctcgttta tcctaggtac gatattactt
      601 agtgtttacc ggatagtcgt ttacaaactg tacttaagag tcagtcgtga taagaaagtc
      661 cttatcgtgg gtttggagaa agatgtcgca ccagctatcc aaaattttca atctaagaaa
      721 aacaataaac ataaggttga agcagtggca atcgataatt attacgaaaa tacaaaaaaa
      781 ttgatcgatg atatggatat cgtctatttg gcttctaaaa tcccagaaga tgaaaaaatc
      841 aacatttatc aattagtgac taaaaaagag aagaaattat ttttaaatac gacttttgag
      901 aatctgacaa tgataaatcc gaatattatg aattttgaag atgaaagcat cattgaagct
      961 tctgggttca gaatccaacc agaatacgaa ctattcaagc gtctctttga tatcatcgtt
     1021 tcgcttattt tactgatcct cgcctcacct tttatgctaa tcacagcgat tttggtaaaa
     1081 acaacttcac ctggaccaat catctataaa caagtacgta ttacgaaaaa tcaaaaggaa
     1141 ttttcaatct ataaattccg ttccatgagt gcgacagctg aagcaaaatc aggaccagtc
     1201 ttggctaaaa gtaacgatgc acgtgtgaca ccagtaggta aatttatccg tgctgtacgt
     1261 tttgacgaat tacctcaaat ctttaatgta ttgaaaggcg atatgtcgat tgtcggtcca
     1321 aggccagaaa gaccattttt cgttgatcag ttcaatgaag aaaatcctta ttattactta
     1381 agacacaatg tacgtgcggg aattaccgga tatgcacaag tatacggaaa atatgtatca
     1441 gattataaca gtaaactacg ttttgatttg ttgtatatca aaaagtattc cttactatta
     1501 gatttgaaga ttttacttca aacaatcaaa atcttatttg ataagatgtc ctcacaagga
     1561 ctagatgaag atgaaaatat cagttcatta gcagaaagtg catttccaaa agaaaaaacg
     1621 tatcgttaga ttggaaaaat tttgagcaaa gaagtaggag agaacattgg aaaataaaaa
     1681 tataagtata ctgctttctt tgtatgataa agaaaagccg gaatatttaa aggaagcatt
     1741 agctagtatc tttgagcaaa cggtgatgcc ggcagaaatc gtattggttt atgatggccc
     1801 tataaatgag aagcttcagg cagttgtcac ttatttccag caaaaagttc ctcacttgtt
     1861 tacagttgta aaattagaaa aaaatcaagg attggggatt gctttagcaa ctggattaga
     1921 acatactaaa aatcgcttag tggccagaat ggatacagat gacatcatgg aaccacacag
     1981 attggaaaaa caattagaga tcttccaaga atatccagaa atagcgattg ttgggagtaa
     2041 tattgaagaa tttactggca cacttagcaa tgttatcgga agaaggattg tacctgagta
     2101 taatgatgaa atccgtgtgt tttcgaaaag aagaaaccct tttaatcata tgacagtcat
     2161 gtttgataaa aatgctattt tagaagtggg aaactatcgc ccattaattg gatttgaaga
     2221 ttattatttg tgggtaagac tattgaaggc gggatataaa ggatataata tccaagagat
     2281 tctagtacat gctagagcag gtgctgacat gtatgctaga cgtggaggaa gtaaatatct
     2341 tttaccagga attaaaggcc gatatcgcgt atggaaagat ggtctaggat cactaaaaga
     2401 ttttctgata gttatttgcg gacatcttgt aattagttta ttaccgaata aaattagggg
     2461 aaaagtatat gaatcaaaac ttagaaaaac aaactaaact ttctataatc ataccttttt
     2521 acaatgcaaa gaatacttta agaagagctg tagacagcgt cttaacgcaa ggttatccca
     2581 atattgaaat cttgttaatt gatgatggat ctacagatgg tagtagtgac attggtgacg
     2641 aaattgctaa tctagagagt aaagtagcat gttatcactt ggaaaacgct ggtccatcag
     2701 ttgctaggaa ctatgggata aaagaagcaa ctggaaattt ggtaggattt ttagatagtg
     2761 acgattattt tttacctgat tgttttaata atgttttgaa agactaccat aggaatcacc
     2821 ccgatatcat tgcttttgga ttaagtaaag gaaaaaatat agaaaatgca aatttatttg
     2881 ttcccgaaaa gaagattggt tgttcaagtg aagaagcaat tgagcaaatg tttagaagta
     2941 aggcagtgga cttttacgta tggaataaat tttttaaaag ggaattattc aatcatatag
     3001 tttttccaga aaataaatta tatgaggata tggttcctat gtatgaagct tttagggcgg
     3061 ctagtaaaat tgatattttg ccattttcag gtatttttta ttatcaaaat aacgatagca
     3121 tcgtttatca gtcttttaat tcaaaacagt atgataatat tttgcagaga aaaatattag
     3181 ttgatgaagt aaagaaagat tatcctcgac ttattaattt ggccaatgct cggttagtag
     3241 acggatatct ttcaacaggg tttaaaatta ctggcaaaaa tcaaaatcag aaagaaaaac
     3301 aaaaatatta taatcagtct aaaagagaaa taaaaagtat atattcaaaa ataagaagta
     3361 acaatga
//
LOCUS       DAMEET010000147         3352 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.153, whole genome shotgun sequence.
ACCESSION   DAMEET010000147 DAMEET010000000
VERSION     DAMEET010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.153"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            55..1968
                     /locus_tag="PB780_002537"
     CDS             55..1968
                     /locus_tag="PB780_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HDE0734545.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            1973..2974
                     /locus_tag="PB780_002538"
     CDS             1973..2974
                     /locus_tag="PB780_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDE0734546.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(3176..>3352)
                     /locus_tag="PB780_002539"
     CDS             complement(3176..>3352)
                     /locus_tag="PB780_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HDE0734547.1"
                     /translation="FASALILFAIHLCSQPYINNNGILVEPAFFCLPLGFLSLLASAG
                     FGFVAFFKRSKQQI"
BASE COUNT         1241 a          528 c          707 g          876 t
ORIGIN      
        1 ttgtataaaa aatgtttgcg tttacatata attctcctag aaacataaaa aggagtgaat
       61 agcttgagaa caaaacaagt attaaaggga gcgttggtca tcttatttgc tgtaagtaca
      121 agcgtcccgc aatggacagt atatggagag agcatttctt ccgaatcgga atctcgggag
      181 gtagaaacca cagaaactac tgatgctgga aatgaaacga aatcgagtac ttcacaaaca
      241 gaaacaaata ctgaaacaac ggttgagaca tctgttacta gcacagatca agaaacgacc
      301 gctaccacag ataaccaaca aacagaagaa gcaacagcta caaaaatgtc aaacgacgaa
      361 tcctcttctg ctaaagtaag tgacaaagag aatgggacag aaaatgatga tatcggtaaa
      421 ggtgaaaatt tcaatggtaa agaatatttg aattacttcg gtaaacagat gacagaaata
      481 cttaatggac caacagaaga catcaagtca aaattaatag agttattagt cactgagctt
      541 gctgttaatg attatggtgt agataaagag attatcaaac atctgattgc tacttatcta
      601 ttgtacgata tcgcttttgc aaaaaacagt gtgttgatgt cgatgcttgg aggtacagat
      661 gatgggacaa cgcctacaga aaacacaggc ttctatccaa atacaaaaca acttcatcaa
      721 actgtactaa acaaacaaga tggcaaaatg atggatgtat atgcatatta tgtcgatcaa
      781 ggttcagata aaacagtcat gatccatggc ggatttcgag ggaattggaa taacgggatc
      841 gttacagaag aatacaatga tttttataag gccgggtata atttattatt tgttgattcc
      901 agagcgactg gtaatagcgg aggcgattat gtgacctatg gacaatatga gtcagatgac
      961 gtcctttact ggataaatca agaagttcga gagcggccat ctcaaaagat tttactttat
     1021 ggtggttcga tgggagcagc tacgatgatg tctgtattag cgaaagatat tcctgtcaat
     1081 gtcaaaggaa ttattgaaaa ttgcggattt gcatcaattg atgaacaact acgttttacg
     1141 tacagccaga cagtagcccc cgcactaccg gatgccataa aaaatcagtt agacatcatt
     1201 ggggatcaag agcatgaaga tttatttatg ggccttttga aacaatacta ttttgatcag
     1261 gagatgcatt tggatacaaa agctgctcta cctacgattg gaatgtcaga ttcattgcct
     1321 aaattgatta tccatggaac agcagatgat gtagtacctg tgtctaacgc gcaaaaatta
     1381 tatgagttat caggagggta taaagatctt ttattagtag aaggagcagg gcatggaaag
     1441 gcacaggaag ttgaccatgc tgcctacacg aaacatgtaa cggacttttt gaaagtagtg
     1501 ttccatgatc agataaacgt aaagtatgtg gatgaaaaca ataaaagtct actcaaagac
     1561 caggacgaaa tccagcttta cggagcttat ggagaaaatt atatgacaga gcaaaagaca
     1621 ttcgaaggat acgaattagc aaatgttgaa ggaccgactg aagggatttt taatgagacg
     1681 ataccaacta ttattttcta ttacaaaaaa atcccagtag ctccaccaaa aaagcaagat
     1741 ccgacagaga atgcagacaa taagggaaag aatgaacctc gagaggatca atcaagtgat
     1801 gatacgcaac cgattcagat gacagtgaaa gtacaaaaaa agacagatgc aaaaaaaaga
     1861 atgttgccga aaactggaga aaagaaacat tcgcttctca tttattcagg tggattttta
     1921 tctgttagca cgattatcac atggatttgg cgaagaaaga gagggtaaat gaatgaaatg
     1981 gttcaagcga atagtgattg cagtaccagt tatcatcgta gttattttag tcactgtatt
     2041 tttgattaat caaatcacac caaagccgat tagcttgctt gttagaaaac aatttgacac
     2101 aagtgaaaaa gaacaagtat atgcccgacc agatgatttc cagcaaaaaa tcgaacaaat
     2161 cgatataaaa aaagaaatat cctatccttc aaagtacaaa aataataata tggatatcta
     2221 cacaccgaaa gtggaaaaga aaaaattacc gattcttttc tggatgcatg gaggcgcata
     2281 tgtaggaggg gataaaaatg attgtcgtga ttatttggaa tatctatgtt cagacacgca
     2341 gcaaatcatc gtaaatatag attatgaacg atcaccggaa gcaaaacatc ctacaccagt
     2401 tatccaattg aacgaagcca ttcaagcagc taaaaaagag ataaaagatc aagcagattg
     2461 gtcgaatatt tcgattggtg gaaattcggc aggtgcacaa attagtggag aatatttgtt
     2521 agcacttcaa aatgaagaaa ttaaaaaagc tgatcatctt gatccgactt tggagaacaa
     2581 gcagattacg aagtttattt ctttgtctgg tctgttggat ccttctgcac ttactcaagt
     2641 aagtgataaa atctctagct ttctttatga aaaatgtgga tgggcttact ttgataataa
     2701 ggactttgaa caatcagaga aggtcaaaag tttagccttg gtcagacatg cggatcgttg
     2761 gacgcaaaac acattcttaa cggatggaaa tacaaatacg tttacgaaac aaatggaaga
     2821 agtaacttct gtatttgaga aagcgggggt gaaagtcaca aaagtagatt atgcggagaa
     2881 aaatgctgtt ttgaaccatg aatatcagtt tgatttcagc aataaacaag cgcaagaaac
     2941 atatcaaaag ttagtaatgt ttttcaagga gtaaagtctt atgatgcaaa agaggttgta
     3001 atctgtcact tagctccaag tcacaaggag caatttcaat aaacaagctt ttcatggttt
     3061 atgaatattg taacttgtga ctgaggagtt agagatggca aggggcacct tttattcgaa
     3121 atttgggaat atcacacgat tattgtcacg atctctttta cgtaaatttt ataagtcaga
     3181 tttgttgttt tgatcgttta aaaaaagcaa caaatccgaa gcctgcggag gcaagaaggg
     3241 acaaaaagcc gagtggtaag caaaagaatg ctggttccac taatattccg ttgttattga
     3301 tataaggctg ggaacaaaga tgaatagcaa ataaaatcaa tgcactagcg aa
//
LOCUS       DAMEET010000148         3320 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.154, whole genome shotgun sequence.
ACCESSION   DAMEET010000148 DAMEET010000000
VERSION     DAMEET010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.154"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(4..705)
                     /locus_tag="PB780_002540"
     CDS             complement(4..705)
                     /locus_tag="PB780_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDE0734548.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(721..1161)
                     /locus_tag="PB780_002541"
     CDS             complement(721..1161)
                     /locus_tag="PB780_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HDE0734549.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(1161..1736)
                     /locus_tag="PB780_002542"
     CDS             complement(1161..1736)
                     /locus_tag="PB780_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HDE0734550.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(2584..3063)
                     /locus_tag="PB780_002543"
                     /pseudo
     CDS             complement(2584..3063)
                     /locus_tag="PB780_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(3222..>3320)
                     /locus_tag="PB780_002544"
     CDS             complement(3222..>3320)
                     /locus_tag="PB780_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDE0734551.1"
                     /translation="IRKSSTQIGEMIEYSEAVYHTELHPYHIDYET"
BASE COUNT          950 a          707 c          527 g         1136 t
ORIGIN      
        1 tttttagcct aattcttgac cttttgcatg ctggttatcc gtaaaatatt ttccagcagg
       61 ctttccttcc aattcagcta atttttcttg gatctgacgg atcacctttt ttgcatcttc
      121 tggattttct acaaaatcga atttatcccc atccacttgt attttaggac atacatcaaa
      181 atcttcatac cattgattat agcgggtgtt caacgtttca taataggtat ataacgtctc
      241 atcgctttct agctgttcat agtcacgccc acgttttttg atacgttcta gcatcgtgtc
      301 caaagaaact ttgatatgga ccatcaaatc aggccgtttt tttggagcaa catcatctaa
      361 ctcgtttagc atcgtatcta ataagctatc gtactgctgt acttcgatat ccgttacccg
      421 acctaaatca gcgtttagat ggaacaacag gctatcttca tagatggatc ggtctagcac
      481 attgtcatcg ttcactaaag catttttcat tgctaaaaat cgcttgttca aaaagaagat
      541 ttgcaataaa aaagcgtact gctcaggatt tgaataaaag agcggcaata cttcgttatc
      601 ttcgacattt tcataaaatg gtttagaatc aatctctttt gcaagcatat ccgtcaaact
      661 gctcttacct gcaccaatcg ttcctgaaat caaaatcact gacatacaac ccttcctctt
      721 ctataagtac tcgcctacgt aagtttcaac aggcaatgtt tcaaaatcat attcacgtac
      781 agcttggtct gattttaaat acgtatggag actttgtgta atcatcaagt tcgtaggtac
      841 cgtctcttct tgtacaatga tcatcggttt tttcaacata gttgccacac ctaattcgta
      901 agccgttcct ggatcgattg tttgatgctc aaagtcaagg atagcaacaa caaattctgc
      961 atttgtcagt tcttccatat ctttttcgta tacttcctgc gcccatccag cgctgaatag
     1021 ttcataattg ctttcttggt gatgacgtgg agagtagaaa cttgtgactg ttttgttttc
     1081 ctccaacgct ttttcgatac gacttactcg atcgatttgc tcctctgaaa aaaatggtcc
     1141 tgctaaatat atttgactca ttattttgcc tccttcaata ataactgatt tttaatagat
     1201 actgctcgta attcattgac cagttccaat ggattgttgg aaaagattac ttgatccaac
     1261 cattttttat ccataaaatc ctcttttgca aaagtttcta ataattgttg caagccgctg
     1321 taataaccgc caatattcaa tattccgatt ggcttgtcgg gcgtcagacc gattgctatc
     1381 caagaaagaa gttgtgagaa ttcctccaat gtacctagac ctccagggaa aacgaggaca
     1441 gcatctccct tttcaaataa taataccttt ctttcatcga tcgtttctgt aggaataaat
     1501 gtagtaacat cttttccagg caattcagtt tcaaaaagcc cattcggata gatacctgtg
     1561 acttttccgt tattttccag tactgtttta ctaatcactc ccataatccc ggagtatgaa
     1621 ccaccgtaaa ccagttcgat ttcttcttgt gccatgtatt ttcctaacgt ttgcgcaata
     1681 aatttgtaac tttctttatt accaaaacgg gaaccgcaaa aaacagtaag tttcataaaa
     1741 aaacactccc taactcaaaa gtctatcttc attctacacg tgttttttta aaataaaaga
     1801 ctagattttg tagtttttta gtggtatata ggatagacgc cactatatat agtatttaga
     1861 aaaaaatcat ttttcagcca ttttatagat aaaacaaaaa taaataacgt gttttcatcg
     1921 atatgaaatg acttacttat agaagatact gggatttcgt cctatatgcc aataacgtgt
     1981 attattttaa acaacaaata cttgtttctt ttattaacga aaaaatcatt ttgttctcgt
     2041 gttatgtttc actgataaat gatgtttctc taaataaaaa agccgacgaa ctaaacgatc
     2101 aaaatttgat catttttgtt catcagcttc tcctaaaaat ctttttgtta acgataaata
     2161 gctggatttg taaaaatatc acttgaatcg tcactgctgg aagaaaattc agtgataacc
     2221 gcacctgttg cgcctgcctg gaactagtgt ttgatattgg gctcgaacgt gtactgatct
     2281 ctagcatgaa gaataatttc tttttttact gtataataac tttctttcct ttttggaata
     2341 gtacaggaaa gtttttttgt ttcctttcct tcgacataag gtataccaga ccctttcgac
     2401 aacgaaaggt tctcattttt ccttcatcgt catttcaagg agggtgccga tgctctggac
     2461 agtttcgatc gctcctaaac caaaatctgt atattcgatt ctttctaact ctgattcttt
     2521 cataccattc ctgtctcaag gtacatctat tgtacttttt tcttgtaatc cattctgcct
     2581 ctcctaacta attgtcaaat catcccaaga tatgtcttct tcctcttttt cttcttggat
     2641 gatttctgca ttcggatcta ctggtttttt caataataaa atggttattc ctgtgacaaa
     2701 agaaccagct aacaatgcac caatgaatgc ccatggtctg gtcatcgtag cgactgcaaa
     2761 cattcccgat gccggaatac ggctatctgc gccccaaaac atgcttaatc cgccacctac
     2821 tgtcccaccg actcccgttg ctatgacatt gcgtgccaag tcgttcaata cgatagggaa
     2881 acatccctct gtgatctgac agatccccat aggaaaagcg gtcttcaatg tatcgatttc
     2941 ttgacgattg agtatatttt tacgaatgat tttccctaag aaataagcga tcgtcaagcc
     3001 gaatggggtt accatcgaag ctaaaatcaa tacagtgatt ggttcataga ttccttctga
     3061 aaatcgtgcc acaccccctt atccagaata aatggtaatc cagaaacagc cgtgtatctg
     3121 cggtaataag cctaaccaaa cgaaaaatat gagaagctat ttttgtttgg ctgttcttat
     3181 tacttgatgc agggcagctt tttcacaacc tcttttttta tttatgtttc ataatcaata
     3241 tggtaaggat gcagctccgt atggtagacc gcctccgaat attcaatcat ttccccaatt
     3301 tgcgtagacg atttccgtat
//
LOCUS       DAMEET010000149         3200 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.156, whole genome shotgun sequence.
ACCESSION   DAMEET010000149 DAMEET010000000
VERSION     DAMEET010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.156"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            302..586
                     /locus_tag="PB780_002545"
     CDS             302..586
                     /locus_tag="PB780_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734552.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            589..1005
                     /locus_tag="PB780_002546"
     CDS             589..1005
                     /locus_tag="PB780_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734553.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1142..1861)
                     /locus_tag="PB780_002547"
     CDS             complement(1142..1861)
                     /locus_tag="PB780_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDE0734554.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2514..2609
                     /locus_tag="PB780_002548"
     CDS             2514..2609
                     /locus_tag="PB780_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDE0734555.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2911..3156)
                     /locus_tag="PB780_002549"
     CDS             complement(2911..3156)
                     /locus_tag="PB780_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734556.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1065 a          598 c          499 g         1038 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tccccccccg aattttaaag agcataagga aacgtgtacc
      121 cactttctaa aaaaagtggg tagataggct gtgcctatca cgtttctcag aaaaaaattt
      181 gatatcactt tttcgccatc gtgggtagct tatgtatagc attttggcaa aaaacagcta
      241 tacaaatata gtatttgtat ggtatacata aattatacat aattgcaaaa ggagattttt
      301 tatggacatt ttagttcgga atattgatcc aaagtttgtt gcaacaatag acaaacgttg
      361 ccaagaactc acgaataaaa caggagaaaa atggagtcga aataaatatc taaaagtttt
      421 aatcgaaagt gattttgacc gtcaacttac gacttacaaa aaagaccaat tcgatttatt
      481 gctcgataaa tttattgcta tccaaacaga aaatactcaa atattaagcg agtatgtcta
      541 cacaaataat caaatcattg aaatgcttat cggaggaatg atataaccat gagaaaaact
      601 tctgtccaaa aacatgttac tttgtaccct agtcatgttg acgttttaga ttccatcatc
      661 gaaaaaaatc cagatatcaa taactactcg gaagccattc gctttttatg tatgagttat
      721 gaacccgata ccaacacgac cactaccagc aatcaggcaa aactaaatgc tatgggaaaa
      781 gaaatttcca tcatgactga aatcatcgca gaatttgctg atttatatct aaaagataca
      841 ggcgttctta ttggcacaaa ttctaaagta tatcaagaag tgaaagaaaa aatcgaagct
      901 aaactaaatc catcaaatcg aaatcgacca aagaatcatc aaaaagaaac cagtcaaaca
      961 gtcacttcta gaaatttcga cccaacaaat tttctaaaac gataaaaaac agataggagt
     1021 tattcctatc tgttttttat ttagaagccc catacaatat gctagaatgt actagcaatc
     1081 ttagtcggtt gtaagggact tgtctacttg cttaatgtaa ggggggcgag ttctttttta
     1141 tttattcttc taaccagtta cgcagagaaa tctttggcaa atctgacgtt aggcttgaaa
     1201 aagtaaattc taatttggcg attttattgc ccttttttgc ttttattttt gtgactttaa
     1261 gatcattgaa gtattttttt agttctcgta atgcaggatt taatactttt tgatcaatgt
     1321 tacccatttg atagtattct gggatatcca ttaagctcct aaactcttca atacgaacta
     1381 cataataacc agtagaacgg tactgcataa gcagtcggaa tagagttttt gcatacgtag
     1441 aacgcaacga cgtgaaagca gataattcaa atctactaaa ttcagctgtt aaaccattaa
     1501 taatatattc taggtccgga ttaacactga cttcaacaaa cttttttgtt ttattgattt
     1561 caaaaccagt aaacaaaaca aagtagcgaa tagaatcctc atcttcagta cggtaagtaa
     1621 gattaagcat atctttatag agcttttcta aatcaccgac aaaggctttt gtagcagtca
     1681 tcttataatc acttaactct tttaattcct cgaaagaaaa tctaactttt ttcaaaccac
     1741 gatctttcat acgagcgcat aatgcaaaaa ataaatccat ctgcttagca ttgaaatttt
     1801 tcagcggtac taaattcaat tcattttgat atttgactgt taattccgtt ttttcatcca
     1861 ttcctttctt ccccccttac aaataaataa taacctttaa caaccaactc gtaaataggt
     1921 tggttgttat tatacaaaag gatattagta ctcagataaa aagttgttag tactcagata
     1981 aaaagttgtt agtactcaga taaaaagttg ttagtactca gataaaaagt tgttagtacc
     2041 cttctgtatc cgttgggaga gtaaggctca actgccccct aaaaagggtt ctaaaaaatc
     2101 tttaaaaaat aaataaaata aacgcgcgcg cgcgagcaaa atttcccgcc aaaaaatcac
     2161 ccacttttta aaataaacag gttcgcgcat gatattaaac tgccaatcta gctccctatg
     2221 gtcgctgtaa gaaggagtga tcgctacgct ctcacatttt acttgcagca gtcgccttta
     2281 ggctccttcg ccaaaaataa ttcattaggg actcctttgt ggcgtcctga gagctcgtct
     2341 aagccgttct aagacaagat tcgataaaaa ctatgtcctt cgttctaaag cgccttacag
     2401 gcggtcaggg caagccctaa caaccccaag gcaataaaaa acttaggctt atttttgctt
     2461 ttttattgta taattgattg tcgcctatcc gacagtcttg cgactggaag gaggtgtgtt
     2521 tcgtgttcac taactttatt tgctcccttc ttgttgggct tttaagcgct ttgttcagtt
     2581 actggttgaa taaccgaaac aagaagtaaa acttaattaa ggcgactcag cccacaaaaa
     2641 aagccccaga gaatccgacc tctctggggc tttcgtgtgt ttcgtgttca cttatttgca
     2701 atcaaattat aacagaaata aatatttatt tcaaaaatat cctacaaaag tctaaaatcc
     2761 gtaattattt cttatatgca acatttacaa ataaagtcgc tctgagagct cgtatttttc
     2821 gatttaagac gttttattta gtttttggtg tatttcattg gtccaagcta aaaaactctt
     2881 agaagcgatt ttagagccat ttaaacccac ttaattatat tctccatgaa attcgctcca
     2941 tgcgtcaaaa gctgcgtctg aaattccgcc gtcttcgttc actagttgca ttaatttttc
     3001 caacgatcgt tttctttcgg gtgtcatatt tttatattcc aacaattttt cgtctgttaa
     3061 atcgaacttg attgcttgaa ttgcttgctc taatttttct aagttttgaa aatattcgta
     3121 aggaaatttt tccatttttt ctttctccag tctcacaatt tttctagaaa taaaatcact
     3181 tctttgccgt ctatactctc
//
LOCUS       DAMEET010000150         3097 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.158, whole genome shotgun sequence.
ACCESSION   DAMEET010000150 DAMEET010000000
VERSION     DAMEET010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.158"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(746..1087)
                     /locus_tag="PB780_002550"
     CDS             complement(746..1087)
                     /locus_tag="PB780_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDE0734557.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1087..1317)
                     /locus_tag="PB780_002551"
     CDS             complement(1087..1317)
                     /locus_tag="PB780_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDE0734558.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1604..1942
                     /locus_tag="PB780_002552"
     CDS             1604..1942
                     /locus_tag="PB780_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734559.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2147..2386)
                     /locus_tag="PB780_002553"
     CDS             complement(2147..2386)
                     /locus_tag="PB780_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HDE0734560.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2362..2709)
                     /locus_tag="PB780_002554"
     CDS             complement(2362..2709)
                     /locus_tag="PB780_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HDE0734561.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
BASE COUNT          963 a          577 c          485 g         1072 t
ORIGIN      
        1 gaactgtatt gatagtgtac ctaaaagctc tactgacata agatcgtgta atcggtaaag
       61 taattttatt aattatacgg gatgtaatca attagagaat aaactaatca taaaaggtac
      121 ttttacattc ataattgtcg ctttgagatt ctactttggt tcactatctg acccaattgc
      181 acattttcga tcattacaag tattggtgtc gttagaacaa ctaaataaaa actagcggta
      241 gatttttcag aatctatgct aatttctgta tggatgttcc agtgatggca aatttctctt
      301 ttcattgttt ttcttgtcgc atgatatgcc tttttgtgtt attcagttaa actatagaaa
      361 ttctttccta gacagatctt gatcccaaat tttacgtttt tgagcatcga tatagtttaa
      421 aatgacatta ctcatatcta aagagtttag attttttagg actaaaactg tcagttgttc
      481 tttttcttct ttctgttaaa gtattgtcgc gacttttcgt atacgttaag atatgtcgtg
      541 aaaaaacaag ttttgtgaca caaaaaaacg aatgacttga ggaaatcacc tcaatcattc
      601 gtttaaaatg ctatatattc tttagtttaa gatacggaaa ttaatgccat cgaccacact
      661 agtagaatga acgacattta cttggccttt ttttaatttc gttatgttta gcatataaaa
      721 atattataga gtttacttac aatgcttaaa acatatactg tataatctga tcaattttag
      781 ccatgtcttg caatggtaag ttttctattt ttttgagttt tcgttcctta aaatcaagag
      841 attttaattg tgtaataatt acttgtcctt ttatatctag ctcttcaggt aatgagtaac
      901 gtgtcggtaa attctttata gtggaagtaa ttggacatat gaccgaaaac attgttttac
      961 ggttaaattc ataacgagag acgactagtc ctggtcgtct tttttgaatt tcttttccta
     1021 cggagggatc aaaatcaatc catacaatat cacctttttt aggaatataa attctttctt
     1081 ctgacactat aaaatttctc tcccttcagg agttaaatct tcccattcgt ctttctcata
     1141 atactcggcc tcttccccac cactaaatgg atcttcaatt tttggaacta acgtaattgt
     1201 tccatcatca gaatacacaa cgatatattc ttggttttct gaaggtttct ttccgttatc
     1261 agaaggtaaa gttaccacta cagaacttcc ttgtaatcta gattttgtaa caagcattat
     1321 catcactccc ttaataaaag tatactatat aacactaagt aacgcaaaat tcccctttgt
     1381 aaactatcta gaaaattgtt ttgtttaaag tatatcttat tggatataaa tagaaataaa
     1441 ttgtagccag ttcatttttt ctttaactaa aaaaataatc caagaaacat tgaacctatg
     1501 gatccaaaag ctacccaaat actatagcta accccccgct accttcaatt ttgcagataa
     1561 aacgctttgg gagaaaattt caaagacaac aggccatcct atcatgcccg attataaaca
     1621 aaaagctggc tattcctttt gctctgacaa atttcaattg ggaaagttca cgaaactagc
     1681 aggtgaagaa gtttcaactg ttcggatcaa agaatgcccg cttcaaatag aaacattggt
     1741 tcaacgaatt tcaactagac aagactttgc tattgtagaa cgtgagatcg tagctatttt
     1801 tgtagaagaa gggattttac atgatccaac tcatatcaat gtggataaat ggcaacctct
     1861 tatctataaa ttcagagaat atacaactgc caaccagtcg ttaactttga attttagatt
     1921 ccaagaattc aaagagtcat aatgataaac atcgttaggt ataaataaaa aagactcgca
     1981 gcacgagtaa aaaacaaata gaagctatac attagaaagg tcgactgtga ccctatagat
     2041 gcagtatatg cagcttatga gcatttcttc tgttctcttt gtttttggga tctgtgctga
     2101 agtctttttc gttttttgcc gctctttctt tgaaaaatcg attatgttat accttctcca
     2161 ttactaattc aggcatctgc tgagtaatga tttttcgcgc aaaagaaata tctgttaccc
     2221 aaggttcgat ttgttcttca gtcaagacaa gcggcattcg gtcatggatt ggtgcgactg
     2281 tgcggttagg ttcagtagtc attatgattg attctgccat tttttcatcc tgatggctac
     2341 gataaaaacc gccaatatac actatttttt ccttatttga aaacataaat ttgtcttttt
     2401 cttcattcca ctcattggga aaacacaacg attttgcaaa aatggttgat gaaaggtctt
     2461 tttttcttct actgtctctg cacgggcatt aatcatcaat tgtccttttt taaatccggt
     2521 aaatccccac ttcatgattt ccggagtgag gatgttttct tcctcttttc ttaacgtcac
     2581 aacaagattt gacggaaaaa cttctccagc tgctaattct gctgcctgct tcggttgttt
     2641 cgtctgggct tctcgataat aattctgaag gagctctgac tctagatcaa aaaaataacg
     2701 accacacatc ctaatccctc ccaatctttt tttctatcat acactgcaac aagaagttcg
     2761 caccaacaaa acgctttcaa tttttgtaga actaaaaaag ttgtgacaag tgacaaactc
     2821 tccagtgaat ggtattctcc tgccaactcc tcggtcacaa gtcacaatat tcaacaaaca
     2881 tgagaagctg ctttttgaaa ttgttccttg tgactcggag tgaaacggca ggcttacgac
     2941 ctctgggtaa acggtgttca aaagccagtt tctcggtatt aagccaaatt tatgcaaaaa
     3001 tgtagaaagc tattttcaca aaatttttct taatactcga aactaagcga ctttttcaca
     3061 acctcttcct taaacggtgt tcaaaagctt gcatctg
//
LOCUS       DAMEET010000151         3046 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.159, whole genome shotgun sequence.
ACCESSION   DAMEET010000151 DAMEET010000000
VERSION     DAMEET010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.159"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            50..805
                     /gene="fabI"
                     /locus_tag="PB780_002555"
     CDS             50..805
                     /gene="fabI"
                     /locus_tag="PB780_002555"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDE0734562.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(915..1928)
                     /locus_tag="PB780_002556"
     CDS             complement(915..1928)
                     /locus_tag="PB780_002556"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HDE0734563.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            2093..3013
                     /locus_tag="PB780_002557"
     CDS             2093..3013
                     /locus_tag="PB780_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HDE0734564.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT          883 a          616 c          580 g          967 t
ORIGIN      
        1 tattttttta cgactatcaa atttttatca gaaacaaagg agagtcatta tgtcatttct
       61 agaaggaaaa aaaatcgtca ttatgggcgt cgctaacaaa aaaagcattg cttggggttg
      121 tgctgaagca atgttgaaac aaggggcaga aattatctat acttaccaaa atgaacgaat
      181 gaaaaaatca ttagtcaaat tggtcggcga agaagcattc actgtagaat gtgatgtttc
      241 tagcgacgaa agcatcgaaa aagcaatgaa tgccatcggt gaatatgctg gggagatcca
      301 cggccttgtc catgcagttg cttacgccaa caaagaagaa ttgtccggaa atgtgtctga
      361 tatttccaga gacggctatc aattagctca agatatcagc agctactctt taattgctgt
      421 atcaaaatac gcacaaccgt ttcttgcacc aaacagcggg atcgttagca tgacttatct
      481 aggttcagaa cgcgcaatcc cgaattataa catgatgggt attgcgaaag cttctcttga
      541 agctgctatc cgttatttgg cagctgagtt ctcgcctaaa ggaattcggg tcaatggaat
      601 ctcagctggg gcaatcaaaa cattagctgt cacaggtgtt aaagactatc aaaaactgat
      661 ccagttatct gaatctcgta cgcctgatca gcaaggtgtg acgatcgaag aagtcggtaa
      721 tgcttgtgct ttcttaatca gcccactttc cagcggaatc atcggtgata ttatctacgt
      781 tgacaaaggt gtccatcttt cataaacttt tgttgcattc tttaaagtaa aacatataaa
      841 aatcaggaaa aaacttttta aagtctcttc ctgatttttt ctttttacgt ttttttcaac
      901 tcattacttt gaaattattc cacgacttca atccagcctt ctggtgcttc tatgtctcca
      961 aattgaatac ctgtcaattc atcatacaac cgttttgtta ctggtcctac ttccgtttca
     1021 ctataaaata catgaagatt gttattgtga taaatcccac cgattggaga aataacagcc
     1081 gctgtaccac atgcaccagc ttctgcaaat tgatctagat catcaatgaa aatatctcct
     1141 tcaattgctt gcagtcctag tcgttcttca gctaactgca acaaggaata tttcgtgata
     1201 cttggtaaga tagagggcga ttgcggagtg atgaattttc cgtcttttgt aattccaaag
     1261 aaatttgctg cacctacttc ttcgattttc gtatgtgtgg ctggatcaag ataaacacaa
     1321 tctgagtaat ccagttcttt tgcttctgtt cctggaagta aactcgctgc gtaatttccg
     1381 ccgacttttg ctgcaccagt ccccttagga gcagcacgat cgtaatcaga tacaataaaa
     1441 ttcgttggtt ttagtccacc tttgaaatac gctcctactg gcgtacagaa gacgacaaat
     1501 aaatattctg gagctggatg gacaccaatg ttttcaccga ccccaatcag taaagggcgc
     1561 aagtataatg tagcgcctgt tccatagggc ggcacaaaat cttcatttgc ttttaccact
     1621 tgcttgacag catcaacgaa catcttttca gggaccattg gcatcagcaa tcgtctagca
     1681 ctttgattca tccgtcgact attttgttca ggtcgaaaga gattgacctt gccgtctttt
     1741 cgacgataag cttttagacc ttcaaaacat tgctgaccat aatgaagcgc tggagagcct
     1801 tcatgaatcg tcaggtaatt atcttctgtc aattctcctt cttcccattg tccgtctttc
     1861 catttggcaa taaaacgcca aggagttttg atataagaaa aacctagatt gttccaatcg
     1921 atttgcatat acttaccacc ttcgttattt tctccacatt ttatcattat ccatattatt
     1981 tgtcatttta ttttcagctt ttttccttca ttatagttat ttttgctatt tccttttttg
     2041 aaaacttctt ggtacaatac agaaagaatc aaattttaga aaagaggatt ttatgttttc
     2101 attattagca actgccggta cgacagactc ttctggcgag acattgacag atcaagctgt
     2161 cagcaaagta aatgcgtttc agcggttttg ggacgggatt gattgggacg ccattttggc
     2221 tgcctttatc caaaaaagtt tatatctgat ttttttgatc gtcctttttt ggattcttca
     2281 ccgtgtggga aaatatctga ttgataagtc ttttaatcag tattctaaaa aagccattct
     2341 aaacgagagc cgcttaaaga ctttacatag tcttttaaat actatttttc attataccct
     2401 tggtttcttt tttatctatg caattttatc tgctatcggt gtgccgatcg gatcactttt
     2461 ggctggtgcc ggaattgcag gtgtggcaat cggtcttggt gcccaagggt tcatgagtga
     2521 tgttattacc ggatttttta ttatcatgga acaacagatg gatgttgggg actacatcaa
     2581 gctagcaaac ttaacgattg aaggaaccgt cgtatctgtt ggtatccgta cccttcagct
     2641 gaaagcaacg gatggtactg tccatttcat cccgaatcga aacatcacca cgatcagcaa
     2701 tctctctcgt gcaaatatgc aagtactttt agatattcgg attgttcctg aagaaggata
     2761 tgacaaaatc tatgagatca ttgatcgagt aaatcgtaca ttagctgaaa aataccaaga
     2821 agagctccaa actgaacctt ctatctttgg attagtggat cttggaaaca gcaattttgc
     2881 gattcgtacg gtctgctacg cattgaacgg aaaacaatat gccttaaaag aagagttctt
     2941 gagctcctat gtcaaagaat tgacagcttc tggtttcacg attccgaaca gtccaattgc
     3001 tgtcggaaaa taatttggac aaacgatgca acgaatctca ctaaaa
//
LOCUS       DAMEET010000152         2947 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.160, whole genome shotgun sequence.
ACCESSION   DAMEET010000152 DAMEET010000000
VERSION     DAMEET010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.160"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(449..865)
                     /locus_tag="PB780_002558"
     CDS             complement(449..865)
                     /locus_tag="PB780_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734565.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGILIGTNSKVYQETKEKI
                     EKQLNPSKRNQPKNRQKETNQTFTSKNFDPTNFLKR"
     gene            complement(867..1148)
                     /locus_tag="PB780_002559"
     CDS             complement(867..1148)
                     /locus_tag="PB780_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734566.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNRYLKVLIEND
                     FDHALMHYKKDQFDLLLEKFIAIQEKNTETLNEYVRTNNQLIETLIGGR"
     gene            1948..2922
                     /locus_tag="PB780_002560"
     CDS             1948..2922
                     /locus_tag="PB780_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDE0734567.1"
                     /translation="MSKRGTIINEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVG
                     TLRENIYYHTPHDVLILDFEQLMRLNEGNGYLKAVGNNGWQLLDQHEENIAYIEILKF
                     QEGKGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKVVPKEIDTWWRLELQ
                     LRRGKATDWHAMVHESLDSFASPHYLPEDVKVTDRMMITALITEHSYWGQIHRNSKYK
                     YRELLKQESQNDELTNHLRETFSESADDLKRELDTWLQGLDVTEVGAE"
BASE COUNT          913 a          438 c          603 g          993 t
ORIGIN      
        1 aaaaaaactt gctgatgaat tgggtgtatc gaaaaatacg gtgcgcaatc aactggtcaa
       61 ttcgggattt gaccttgctc agtataaaga aaaaaatgca gtggtcaatt gaccgaaatt
      121 tatgtctttg ggttttgggg ttttaaaacg aaaaaaaggg ggcgattttc gccccctttg
      181 ctctatgtga aaaatttgtt atacttattt tatccttaaa acataccaat ctttttctta
      241 tttagctgct cgtacgagca gcttttttta cgattatgaa ttcaaatcat gtatttataa
      301 tttttttgtt tttatctttt tttctgttat ttcttctgaa attcgttttt ttcattcaat
      361 tggttactat cttattgtaa atgttgctat taagatggtg atcctaccca aaaaataaac
      421 caggaattta aatctcctgg tttatttttt atctttttag aaaatttgtt gggtcgaaat
      481 ttttagaagt aaatgtttga ttggtttctt tttgacgatt ttttggttga tttcgtttgg
      541 agggattaag ttgtttttca attttttctt tcgtttcttg atacacttta gaatttgtgc
      601 caataagaat acctgtatct tttagatata aatcagcaaa ttctgcaatg atttcggaca
      661 tgatggaaat ttcttttccc atagcattta gtttggcttg gttgctggta gtcgtagtat
      721 tggtatcagg ttcataactc atacataaaa aacgaatggc ttccgagtaa ttgttgatat
      781 ctggatttct ttcgataatg gaatctaaaa cgtccacgtg actgggataa agggtaacgt
      841 gtttttgaac agaagttttt ctcatgttat cttcctccga taagtgtttc aatgagttgg
      901 ttgtttgtac gaacatattc attcagcgtt tcagtatttt tttcttgaat agcgataaat
      961 ttttcgagta ataaatcgaa ttgatctttc ttgtaatgca taagtgcgtg gtcaaaatcg
     1021 ttttcgatta ggacttttaa gtagcgattt cgactccatt ttttgccagt tttatttgtg
     1081 agttcttggc aacgtttgtc tatttttgca ataacctttg aatcgatctt ccgaactaaa
     1141 atgtccatag aaaaactcct ttcgcatttg tgtataattt atgtatatca tacgaatata
     1201 gtatttgtat agctgttttt tgctaaaata ctatacataa gctagccacg atggcgaaaa
     1261 agtgatatca aatttttttc tgagaaacgt gataggcaca gcctatctac ccactttttt
     1321 tagaaagtgg gtacacgttt ccttatgctc tttataaaat ggtgatgtgc gacttttttc
     1381 tgttcgtaaa aataaaaaaa gtttacgaaa atgaaactaa actgaacgat tatgacgggc
     1441 atgtaatcaa gatgaaatga ttttataagc gcgtattttg cgttctaaga cgttttagcg
     1501 ttcatttcgt ttagttatcg gcacaatcgt taaaacacgt aagagaagcg agaatggagc
     1561 ctgtaaggcg actggtagcg aaacggcgtg ttagattgtg caagccgaga actgaatgaa
     1621 atgaagagcg gaagcgtgaa ataatttcgg caggaggagg ctcaaggggt tcggggaatg
     1681 aaattccctg aggtcttttt atttttttaa gaggagtgct ataatcaaag aaaacgagaa
     1741 aaaagagcaa aataaaagcc gaacggtgtt ggaagcaccc ttcggctaat tacggaaact
     1801 actcacctag ttccgtaact caaattcatt atacgaatat ttttaggttt cttcaagcta
     1861 gagatttttg gatatggatt tttagagtta cgtgaactct ggaaattctt tccaaaaatc
     1921 tcttttttct ttgaggagga acaaaatttg agtaaacgtg gcacgatcat aaatgagtgg
     1981 gaaaaactta accgcatatc taacaggcgg ttaaatgcac aaactatgtg caccccctta
     2041 aaaatgcaat ggtcaattga ccgaattacc attgttggta cattaaggga aaatatttat
     2101 tatcacactc cccatgatgt gttgatttta gattttgaac agcttatgcg tctaaacgaa
     2161 ggtaacggat atttaaaagc ggttggaaat aatggctggc aattactaga ccaacatgag
     2221 gaaaatatag cttatattga gattcttaag tttcaagagg gaaaagggcg gattgacttt
     2281 aaccccaata aaattagcca atttctggcg ggctcgatga agaactttat ccatgattta
     2341 tttttagaac cacatttctc acgtgcagat attgcttgtg acattattga cgtgccagat
     2401 gatttcatta cccaatatcg ggtggttgat cctgtttctt tcaaaccaat ttacggtcgg
     2461 tctgggaaac tggaaacagc atattgggga agtcgtgcta gtgaacgaca gattcggatg
     2521 tataacaaaa aattggaaca agaaagaaaa cggaaagttg ttccgaaaga aatcgatact
     2581 tggtggcgac tagaattgca attgcgaagg gggaaagcaa ctgattggca tgcaatggtt
     2641 catgaaagtt tagatagctt tgcaagtcct cactatttgc cagaagatgt gaaagtaact
     2701 gacagaatga tgattactgc tcttataacg gaacatagtt actgggggca aattcataga
     2761 aattcaaaat ataaatatcg tgaattgtta aagcaagaaa gtcaaaatga tgagttgacg
     2821 aatcatttgc gagaaacatt ttcggagtcg gcagatgatt tgaaacgaga attagataca
     2881 tggcttcaag gacttgatgt gacggaagta ggtgctgaat gagtgattcg atagaaaaaa
     2941 tgaccct
//
LOCUS       DAMEET010000153         2939 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.161, whole genome shotgun sequence.
ACCESSION   DAMEET010000153 DAMEET010000000
VERSION     DAMEET010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.161"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            92..1423
                     /locus_tag="PB780_002561"
     CDS             92..1423
                     /locus_tag="PB780_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HDE0734568.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            1445..2071
                     /locus_tag="PB780_002562"
     CDS             1445..2071
                     /locus_tag="PB780_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDE0734569.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            2254..2835
                     /locus_tag="PB780_002563"
     CDS             2254..2835
                     /locus_tag="PB780_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDE0734570.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
BASE COUNT         1022 a          474 c          643 g          800 t
ORIGIN      
        1 tgacaaataa agcagatgat ttggagaata gacaattatc ggttatcata tttttgtacc
       61 aatcggtagg taaaaaagaa gcgagggaaa aatgatggaa aaatatcaaa atattgtgat
      121 cggttttgga aagggcggta agactttagc gaaggcgtta gcttcaaaag gtgaatcggt
      181 tttagtcgtt gaaaagtcaa cacgtatgta tggcggaaca tgtatcaaca tcggctgtat
      241 tccttctaaa tcattgattt tcaatggaga aagaggaatc gactttacag aggctgttgc
      301 tcggaaagaa aaattgacgg gcatgcttcg cgccaaaaat tatcatatga tcagcgatga
      361 ggcaactggg acagtgatgg acggtacagc acgttttctt tctaaccatc aaatcgaagt
      421 gacgaataat ggtgaaaaag tgattgttga aggcgaacgg attttcatca atactggttc
      481 tgaaccaatc attttaccaa tcaaaggatt gaatacaagt cgctacttga ttgattctac
      541 gcaagcgatg gatcaggaac aattaccgga aaaattagtg atcatcgggg ctggatatat
      601 tggcttggaa tttgcatcaa tgttcaatga atacggatct aaagttgtcg ttcttgatgc
      661 ccattctgaa tttcttccac gtgaagatga agatatcgcg caaatgattt tagaagacat
      721 gacaaatgcg ggaatcgaat tccatctagg tgtatctgta gatcaagtag cagaccaaga
      781 atcaacagct gccgtcacct ttacagaaga tggccaagaa gtaacaatcc aagcgtcaaa
      841 agttttggct gcaactggaa gaaaaccaag tacagcatca cttggattag aaaatacaga
      901 tgttaaacta acagatcgcg gagcaatcgc tgtggatgaa taccttcgta cagatgcaga
      961 caacatttgg gcgattggag acgtcaaagg cggattgcag ttcacttata tctctctaga
     1021 tgattatcga atcattatgg atcagttgaa gggcgagaat aaacgaacaa cgaataaccg
     1081 gaaagctgtg ccttacagtg tatttatcac tccgacttta tccaatgtag gtttaacaga
     1141 aaaacaagcc aaagcacaag gcgtcaatta caaattattc aagtatatga caagcggtgt
     1201 ccctaaagca caagtattag aagatcctaa aggtgtattc aaagttttag tcgaccctga
     1261 aactgacttg attctagggg caagtatcta tgcggaagat tctcatgaag tcatcaactt
     1321 gatttcgcta gctatgaatg gaaaacttcc ttatacgtta ttgagagatc aaatctattc
     1381 tcatccgaca atgagtgaag ctctaaatga tgtattaaaa taaataacga atggagaatc
     1441 caccatgttc aataataaaa atacagcagt agttctcact gacccgcaag tcgagttctt
     1501 gaaaccagca ggcggtggct ttggtttgac aaaagatatc ttagaaaaat acaatacgat
     1561 cgaaaatatg attgaaatgg taaaacaagc aaaagaaaaa ggatacaaac tctttatttc
     1621 tcctcattat ttttacccac atgaccataa atggcaattt ggcggtgcag gagaacatat
     1681 gatgcttgag cagcagatgt ttgctagaaa aagtcaatat gaatcagtgg aagaaggttc
     1741 aggagccgat tttatcgaag aattgaaacc ttatttggat gaaaatacga tcatcagctc
     1801 tcctcataaa atctttggac cagaaagcaa tgatttggcg ctccaattga gaaaaaatgg
     1861 aatcgacaca gtaattcttg gtggaatgaa cggaaacttg tgtagcgatt cccatatgcg
     1921 tgaattggta gagagtggat tcaatgttgt ggtagcgaca gatgcaattg gtgctcctgg
     1981 ccaagaagca atggatgcag cgttgacgaa ctttggcttt attgcagatc aagtgatgac
     2041 aacagaggaa gtattagagc aactatcata agataggaat tgcttttgtc ctcgcagttc
     2101 ttgaatcaat gaacatctag ctatatgaga acagttttat agtgagggct gtgtcagata
     2161 atttggcaca gtctttttta aacgattaag aagaaaaaat gatgaattta ctgtataata
     2221 taggactatg agaaatgaaa aaggagaata tcattgaaaa caaaggaaaa gatcttggaa
     2281 gcgaccaaag atcttattta taaaaaaggc tatcatgaaa cgtcaatccg agatattcta
     2341 gcagcttctg atacaggaaa aggtcaattt tattattact tttcttcaaa aaaagaagtt
     2401 tgtctagcgg tgatccatgc gcatatggac gtatggcaga cagaattgtt cgaagacatc
     2461 ttaaatgctg gaaaacagcc agaagaagca ttagaagaaa tgttggactg gattttcgct
     2521 gatcatgaga cacagatcac ttatcatggc tgtcctgtag gtaatttgat tattgagttg
     2581 agtacagtgg atgaagaata ccgtctgttg ctactagaat tagtcaatca atgggtcaag
     2641 tatacgaaac aaaatatcct ggcactttct gaagaaaaac tgtccgatga agaagcagac
     2701 cgtcaagcca aagcgttgat tgctgaaatc cagggaagta tcttattgtt gaaagtcaca
     2761 caggatctat cactacttag gcagaatttt caattgatca aagaaagatt gttaacttcg
     2821 caaaaggttc tttaataatt agaggttgtg acagaagtgg tcagcttcaa gaaataagaa
     2881 ggaatttacg aaaattgctt ctcaaatttt tgtgaaattt cggcttattt ttcgaagaa
//
LOCUS       DAMEET010000154         2931 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.162, whole genome shotgun sequence.
ACCESSION   DAMEET010000154 DAMEET010000000
VERSION     DAMEET010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.162"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            88..984
                     /locus_tag="PB780_002564"
     CDS             88..984
                     /locus_tag="PB780_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDE0734571.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIVHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMLERIIQLENEKE"
     gene            1082..1291
                     /locus_tag="PB780_002565"
     CDS             1082..1291
                     /locus_tag="PB780_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HDE0734572.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            1309..1581
                     /locus_tag="PB780_002566"
     CDS             1309..1581
                     /locus_tag="PB780_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDE0734573.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILTKEKVA"
     gene            1583..2446
                     /locus_tag="PB780_002567"
     CDS             1583..2446
                     /locus_tag="PB780_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HDE0734574.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELVKLYEKDVVKYVTPYSNRMTEAI
                     ISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTP
                     PIPKTPKLPGI"
BASE COUNT         1163 a          464 c          509 g          795 t
ORIGIN      
        1 aaagagggct tttatatatt aatcacaaat cacttatcac aaatcacaag tgattaatca
       61 caaatcactt gtgatttgtg attcttaatg atacaatatt actatacaaa aaaagaatgg
      121 ggcgtagtta tggagaagga aaaactaaaa atacttgaag aattaagacg tattttaaac
      181 aataaaaatg aagcaattat tatcttgaat aattacttta aaggtggtgt tggaaagtcc
      241 aaattatcga ctatgtttgc ttacttgaca gacaaattga atttaaaagt tttaatgatc
      301 gataaggact tacaggcaac attgacaaaa gacttagcaa aaacttttga ggtagaattg
      361 ccacgtgtca atttttatga aggcttgaaa aatggaaact tggcttcttc tattgttcat
      421 ttgactgata atttagactt gatccctggc acgtttgatt tgatgttact gccaaaatta
      481 actcgctcat ggacttttga aaatgaaagt agattgcttg ctactctttt agcaccttta
      541 aaaagtgact atgatctcat tattattgat actgtaccaa cgccaagcgt ttatacaaat
      601 aatgcaatcg tggcgagtga ttacgttatg atccctttac aagcagaaga agaaagtaca
      661 aacaacattc aaaactatat ttcctatttg attgatttac aagaacaatt taaccctgga
      721 ctagatatga tcggttttgt tccttattta gttgatacgg acagcgcaac gataaaatca
      781 aacctggaag aactgtacaa gcaacataaa gaagataact tggttttcca aaatattatc
      841 aagcgaagta ataaagtaag tacctggtct aaaaatggaa ttacagaaca caaaggctat
      901 gacaaaaaag ttttatccat gtatgagaac gtattttttg aaatgcttga gcgaattatt
      961 caattagaaa acgaaaaaga atagaatcac aaatcacaag tgattaatca caaatcactt
     1021 gtgatttgtg attgttgatg ataaaataag aataagaaga aatagaaaga agtgagtgat
     1081 tgtgggaaat ttaggcgcac aaaaagcaaa acgaaatgat acaccaatca gtgcaaaaaa
     1141 agatataatg ggagataaga cggttcgtgt tcgtgctgac ttacaccata ttataaaaat
     1201 tgaaacagca aaaaatggcg gaaacgtaaa agaagttatg gatcaagcct tagaagaata
     1261 tatacggaaa tatttacctg ataaacttta aaaaggagtg aaaatgaaat ggcagttacg
     1321 tatgaaaaaa catttgaaat agagatcatt aacgaattat cggcaagcgt ttataatcga
     1381 gtattaaact atgttttgaa ccatgaacta gatactaaaa atactcgttt actagaagtg
     1441 aatcttttaa atcaattaga agtggcacaa gaagttgatt tatttcaaca accatttgaa
     1501 gaattacaag ctattcatga gtattggcgg tcaatgaatc aatattcaaa acaaattttg
     1561 acaaaagaga aagtggctta acatggcgaa tattactgac ttcaccgaaa agcaatttga
     1621 agatcgttta gaaaagaatg ttgaacgact aactaaaaat agactagcgg ttgaatcgcc
     1681 aaccgctttt ttacttggtg ggcaaccagg atcaggtaaa actagtttgc gatcggcaat
     1741 ttccgaagag acacaaggaa atgttgttat cattgataat gatacgttca aacaacagca
     1801 ccctaatttt gatgaactag tgaaacttta tgaaaaagac gtagtaaaat acgttacccc
     1861 ttattctaat cgcatgacag aagcgatcat aagccgtttg agagataaag ggtataattt
     1921 agtgattgaa ggtacaggac gaacaacaga cgttcctatt caaaccgcaa caatgcttca
     1981 agccaaagat tatgaaacaa aaatgtatgt catggcagta cctaaaatca actcatattt
     2041 aggaacaatt gaacggtatg aaactatgta tgcagatgat ccaatgacag ccagggcaac
     2101 accaaaacaa gcgcatgata ttgttgtcaa aaacttaccg accaatttag aaacccttca
     2161 taaaacgggc ttatttagcg atataaggct ttacaataga gaaggagtga aactctattc
     2221 aagcttagaa acgccttcca ttagtccgaa agagacctta gaaagagaat taaatcgtaa
     2281 agtatcaggg aaagaaattc aaccgacttt ggagagaata gagcaaaaaa tggttcaaaa
     2341 tcaacaccaa gaaacccctg aatttaaagc aattcaacaa aaaatggaaa gcttgcagcc
     2401 acctacacca ccaataccca aaacacctaa acttccaggg atttaaacct taaaaaagta
     2461 aaagagtagt taccaaaaaa cggtaactac tcttttttta taaaaacatt tcctcatttt
     2521 ttaaaagctg atcgagaaat aaagcacaca actgtattct ttcttttttc agttggtaca
     2581 ttttgcagtc tgtaacgtgc catttactgt ttgttacctt aacatatcca gtcgaaaaca
     2641 caagggtata taaacgagca agctcgccct tgtgttttct ttgtgtctat gttgcgttct
     2701 cgtcacagca aacgacacga acacactggc gaaaatgaac caacaaaacg aaaaaagaaa
     2761 ggagcgtaca ctgtaattta tttctagctt ttaaaacatg aggaaaacag cttcacttcc
     2821 tcaaacgcct aacaaaaatg gttgtcaaaa aactaaaaat caaaaaaaga aaagaggaaa
     2881 agaaaatgga aaaaacaaaa aaattacaat tagaagattt tacagaaaat g
//
LOCUS       DAMEET010000155         2857 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.163, whole genome shotgun sequence.
ACCESSION   DAMEET010000155 DAMEET010000000
VERSION     DAMEET010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.163"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            562..843
                     /locus_tag="PB780_002568"
     CDS             562..843
                     /locus_tag="PB780_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734575.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLEKFIAIQTKNTQVLNDYVHTNNQLIETLIGGK"
     gene            845..1261
                     /locus_tag="PB780_002569"
     CDS             845..1261
                     /locus_tag="PB780_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734576.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKT
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1422..1688)
                     /locus_tag="PB780_002570"
     CDS             complement(1422..1688)
                     /locus_tag="PB780_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734577.1"
                     /translation="MAFRFRCDRGGRKMTEKFPYEYYQNLEKLESAIQLVKLDLLDEN
                     LLKYKNITPERKGKLKKMQQLFDEEDGISDVIFDTWSEFHGEYN"
     gene            complement(1646..2620)
                     /locus_tag="PB780_002571"
     CDS             complement(1646..2620)
                     /locus_tag="PB780_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDE0734578.1"
                     /translation="MGKKRTIVNEWEKLTAPSNRRLNVGESVKMIKMDWSIDRITIVG
                     TLNENIYYHTQNDVLILDFEQLMRVNEGNGHLKAVGNNGWQLVDQHEENIAYIEILKW
                     QEGKGRIDFNPNKISQFLASSMKKFIHDLFLEPHFSRADVACDLIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQEAKRKIVPKEVETWWRLELQ
                     LRRGKATDWYEMVQESLDSFASPHYFPEDVKTLDRVMIKGLLYDHSEWSTISRDLKYR
                     LRKLLKQESQNDELTNHLRETFEESADELKRELDTWLLGLDVTEEEEK"
BASE COUNT          926 a          624 c          397 g          910 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatcattt cacgcttccg ctcttcattt cattcagttc tcggctttca caatctaaca
      121 cgccgtttcg ctaccagtcg ccttacaggc tccattctcg cttctcttac gtgttttacc
      181 gattgtgccg acaactaaac gaaatgaacg ctaaaacgtc ttagaacgca aaatacacgc
      241 ttataaaatc atttcatctt gattatatgc tcatcataat cgttcaattt gacatcattt
      301 ccgtaaactt tttttatttt tataagcaga aaaaagtcgc acatcaacat ttttttaaag
      361 agcataagga aacgtgtacc cactttctaa aaaaagcggg cagataggct gtgcctatca
      421 cgtttctaag aaaaaaattt gatatcactt tttcgccatt gtgactagct tatgtatagt
      481 gtttcagcaa aaaacagcta tacaaatact atattcgtat gatatacata aattatacac
      541 aaatgcgaaa ggagtttttc tatggacatt ttagttcgga agattgattc aaaggttatt
      601 gcaaaaatag acaaacgttg ccaagaactc acaaataaaa ctggcaaaaa atggagtcga
      661 aataaatatc taaaagtttt aatcgaaagt gattttgacc gtcaacttac gacttacaaa
      721 aaagaccaat tcgatttatt actcgaaaaa tttatcgcta ttcagacaaa aaacactcaa
      781 gtactaaacg actatgttca tacaaataat caacttattg aaacacttat tggaggaaaa
      841 tagcatgaga aaaacttctg ttcaaaaaca cgttaccctt tatcccagtc acgtggacgt
      901 tttagattcc attatcgaaa gaaatccaga tatcaacaat tactcggaag ccattcgttt
      961 tttatgtatg agttatgaac ctgataccaa cacgactact accagcaatc aagccaaact
     1021 aaatgctatg ggaaaagaaa tttccatcat gtccgaaatc attgcagaat ttgctgattt
     1081 atatctaaaa gatacaggcg ttcttattgg cacaaattct aaagtgtatc aagaagtgaa
     1141 agaaaaaacc gaagctaaac taaatccgtc gaatcgaaat cgaccaaaga atcatcaaaa
     1201 agaaacgagt caaacagtca cttctagaaa tttcgaccca acaaattttc taaaacgcta
     1261 aaaaataaaa cgagaatttt tttatttctc gttttatttt ttaggaaagc cggtcttaca
     1321 gcaagatttg caagttaccg gtaacttgcg gaaaagaaga agccgctcac gcgacttctt
     1381 ctttttgcat tttctgaaag ctaccacaat tcctaacttc gctaattata ttctccatga
     1441 aattcgctcc atgtatcaaa aatcacatct gaaattccat cttcttcatc aaataattgc
     1501 tgcatttttt tcaatttacc ctttctctct ggcgtaatat ttttgtattt tagtaaattc
     1561 tcatccaaca aatccagctt gaccaactga atagcactct ccaatttttc tagattttga
     1621 taatactcgt atggaaattt ctcagtcatt tttcttcctc ctctgtcaca tctaaaccta
     1681 aaagccatgt atccaattct ctttttaatt cgtctgcaga ctcctcaaat gtttctcgta
     1741 aatgattcgt caattcatca ttttgacttt cttgcttcag caatttcctt agccgatatt
     1801 tcaagtcacg actaatagtt gaccactcag aatggtcata caacaacccc tttatcatca
     1861 ctctatccaa cgtttttaca tcttcaggaa aataatgcgg actagcaaaa ctatccaaac
     1921 tttcttgaac catctcatac caatcagtcg cttttccacg gcgcaattgt aactccaaac
     1981 gccaccacgt ctcgacttct ttaggtacaa tttttcgctt tgcttcctgt tccaacttct
     2041 tgttgtacat ccgaatttgg cgttcgctgg cacgactacc ccaataagcc gtttctagct
     2101 taccagagcg tccataaatt ggtttgaagg acacagggtc aactacacga tattgtgtaa
     2161 taaactcatc aggcacatca ataagatcgc aggcaacatc cgctcgtgaa aaatggggct
     2221 ctaaaaataa atcatgaata aattttttca tagagcttgc taaaaattga ctaattttat
     2281 tggggttaaa gtcaatccgc cctttaccct cttgccattt caaaatttcg atataggcaa
     2341 tattttcttc gtgttgatct accaattgcc aaccattatt cccaacagct tttaaatgac
     2401 cattaccttc gttcactcgc attaattgct caaaatctaa aatcaacaca tcattttgag
     2461 tgtgataata aatattttca ttcaaagttc cgacaatcgt aattcggtca atcgaccaat
     2521 ccatttttat catttttacg ctctcaccga catttaaccg cctgttagag ggggctgtta
     2581 atttttccca ctcattcaca attgttcttt ttttacccaa attttgttcc tcctcaaaga
     2641 aaaaaagaga tttttggaaa gaatttccag agttcacgta actctaaaaa tccacatcca
     2701 aaaatctcta gcttgaagaa acctaaaaat attcgtataa tgaacttgag ttacggaact
     2761 aggctagtag tttccgtaaa ggtcgagagt gttagcgcac tgctcgacta tttttttgct
     2821 cttttttctc gttttctttg attatagcac tcctctt
//
LOCUS       DAMEET010000156         2777 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.164, whole genome shotgun sequence.
ACCESSION   DAMEET010000156 DAMEET010000000
VERSION     DAMEET010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.164"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(15..>2777)
                     /locus_tag="PB780_002572"
     rRNA            complement(15..>2777)
                     /locus_tag="PB780_002572"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          581 a          836 c          572 g          788 t
ORIGIN      
        1 tttcttttaa cctattggtt aagtcctcga tcgattagta tcagtccgct ccatacatca
       61 ctgtacttcc actcctgacc tatctacctg atcatctctc agggatctta ctttcttaaa
      121 gaaatgggaa atctcatctt gaggtgggct tcacacttag atgctttcag cgtttatccc
      181 ttccctacat agctacccag caatgccctt ggcagaacaa ctggtacacc agcggtaagt
      241 ccatcccggt cctctcgtac taaggacagc tcctctcaaa tttccaacgc ccgcgacgga
      301 tagggaccga actgtctcac gacgttctga acccagctcg cgtgccgctt taatgggcga
      361 acagcccaac ccttgggacc gactacagcc ccaggatgcg acgagccgac atcgaggtgc
      421 caaacctccc cgtcgatgtg gactcttggg ggagataagc ctgttatccc cagggtagct
      481 tttatccgtt gagcgatggc ccttccatgc ggaaccaccg gatcactaag cccgactttc
      541 gtccctgctc gacttgttgg tctcgcagtc aagctccctt ctgcctttac actcttcgaa
      601 tgatttccaa ccattctgag ggaacctttg ggcgcctccg ttacctttta ggaggcgacc
      661 gccccagtca aactgcccat ctgacactgt ctcccaccac gattagtggt gcgggttaga
      721 gtggccataa cgcaggggta gtatcccacc agcgcctcca tcgaaactag cgttccgatt
      781 tctacggctc ctacctatcc tgtacatgcg gtacagacac tcaatatcaa actacagtaa
      841 agctccatgg ggtctttccg tcctgtcgcg ggtaacctgc atcttcacag gtactaaaat
      901 ttcaccgagt ctctcgttga gacagtgccc aaatcgttac gcctttcgtg cgggtcggaa
      961 cttacccgac aaggaatttc gctaccttag gaccgttata gttacggccg ccgtttactg
     1021 gggcttcaat tcgtaccttc gcttgcgcta agcactcctc ttaaccttcc agcaccgggc
     1081 aggcgtcagc ccctatactt catcttacga ttttgcagag acctgtgttt ttgataaaca
     1141 gtcgcttggg cctattcact gcggctgatc gtatgatcag caccccttct cccgaagtta
     1201 cggggtcatt ttgccgagtt ccttaacgag agttctctcg ctcaccttag gattctctcc
     1261 tcgactacct gtgtcggttt gcggtacggg ccgctgtttt ctcactagaa gcttttctcg
     1321 gcagtgtgac atcaggaact tcggtactat tatttccctc cccatcacag cttgtcctta
     1381 agaaaagaag catttgactc ctcttcagac ttactgcttg gacagacatt tccgatcgtc
     1441 tgcattcctt agcctcctgc gtccctccat tgctcaaaca aaaacaacgg gtacaggaat
     1501 atcaacctgt tatccatcgc ctacgcctgt cggcctcggc ttaggtcccg actaaccctg
     1561 ggcggacgag ccttccccag gaaaccttag tcattcggtg gacaggattc tcacctgtct
     1621 ttcgctactc ataccggcat tctcacttct aagcgctcca gcagtcctca cgatctgcct
     1681 tcaacgccct tagaacgctc tcctaccaat acacctaaag gtgtactcca cagcttcggt
     1741 aatatgttta gccccggtac attttcggcg cagggtcact cgactagtga gctattacgc
     1801 actctttaaa tggtggctgc ttctaagcca acatcctagt tgtctgtgca accccacatc
     1861 cttttccact taacatatat tttgggacct tagctggtgg tctgggctgt ttccctttcg
     1921 actatggatc ttatcactca cagtctgact cccggatatg aatgaatggc attcggagtt
     1981 tatctgaatt cggtaacccg agatgggccc ctagtccaaa cagtgctcta cctccatcat
     2041 tctcaattcc gaggctagcc ctaaagctat ttcggagaga accagctatc tccaagttcg
     2101 tttggaattt ctccgctacc cacacctcat ccccgcactt ttcaacgtac gtgggttcgg
     2161 tcctccagtg cgttttaccg caccttcaac ctggacatgg gtagatcaca tggtttcggg
     2221 tctacgacta catactcaaa cgccctattc agactcgctt tcgctgcggc tccgtctctt
     2281 caacttaacc tcgcatgcaa tcgtaactcg ccggttcatt ctacaaaagg cacgccatca
     2341 cccattaacg ggctttgact tgttgtaggc acacggtttc aggttctatt tcactcccct
     2401 tccggggtgc ttttcacctt tccctcacgg tactggttca ctatcggtca ctagggagta
     2461 tttagccttg ggagatggtc ctcccggatt ccgacggaat ttctcgtgtt ccgccgtact
     2521 caggatcctc ctaggtgtct tccacatttc gtctacgggg tttttaccct ctttgactga
     2581 cttttccaag tcattcgact atctgaaaga actaccatat tggagtccta caaccccaac
     2641 aagcaagctt gctggtttgg gcttttcccg tttcgctcgc cgctactcag ggaatcgaat
     2701 tttctttctc ttcctgcagg tacttagatg tttcagttct ctgcgtctac ctcgaatgtg
     2761 ctatgtattc acacaat
//
LOCUS       DAMEET010000157         2692 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.165, whole genome shotgun sequence.
ACCESSION   DAMEET010000157 DAMEET010000000
VERSION     DAMEET010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.165"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            51..260
                     /locus_tag="PB780_002573"
     CDS             51..260
                     /locus_tag="PB780_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734579.1"
                     /translation="MICYISFLILGLPQAVFPEMFSGDWGIVSYSILTLIAMVLGTSF
                     YNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            467..667
                     /locus_tag="PB780_002574"
                     /pseudo
     CDS             467..667
                     /locus_tag="PB780_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1379..1984)
                     /locus_tag="PB780_002575"
     CDS             complement(1379..1984)
                     /locus_tag="PB780_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDE0734580.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2166..2501
                     /locus_tag="PB780_002576"
                     /pseudo
     CDS             2166..2501
                     /locus_tag="PB780_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
BASE COUNT          887 a          438 c          470 g          897 t
ORIGIN      
        1 atttaaaaag aaaagtgaca gataggaata aaaacttatt aaatgtagtt atgatctgtt
       61 atatttcatt tctaatacta ggtcttccac aagcagtgtt tcctgaaatg ttttctggag
      121 actggggtat tgtcagctat tctatattaa cattgattgc tatggtttta ggtacttcat
      181 tttataatgc ttttgtaagt aagcatgttg aggaatatga agtattaatg tacttaaaag
      241 agaaaaaaac aagtaattaa tatgagaaca ccacgcatag tttttatttt gagatattct
      301 agaatgaaac acattaagaa cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc
      361 taagtttttt catatttgtt tgttatgcta tacatagatc tgcataatta gacttgcgct
      421 gcccgttatt gaagtttcag gcagcgctct ttttggggga tcgataatgg aagaagaaaa
      481 aatatttgag aaacgctggc agctagcatc aagtgaacaa agagcaagat acaataattt
      541 gatcacctcc taccccactg ttgactagac atataaggaa aagaaatact tactttggct
      601 gtgccagtta gatatagata cctttgaaac atttgaagtg atcctagata aaattaaaca
      661 gaattgatca gaaacaagcg aagccttctc tcggttcgct tgttttgcta tttattagtt
      721 ggctcagggc aactctaagc tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta
      781 ccactttaga atacatggat gtacgtattg ccattgactc ctattagtta ctgaaaaaaa
      841 atttaaacaa gttgagtatt gcagataaat agctccagtg aggtgaataa ctgctccact
      901 caaggcaata gatagctcca tccagggcaa caaactgctc cactttcata tttaatagat
      961 gaataacctt ctgtataata gaaaggcata cctttcggta tgacaactta tacagaatgt
     1021 tattttattg gttcactatc gaaaaatttt ggagctttat ttttccggta tttctcaacg
     1081 aacgattagt ttaactgtgg gaagctccag acatacgatt cgagaagtta cttctcgagc
     1141 agacaggaag ggggtgactg aactaaccgc tgatatgagc gatacttggc tgtaggaata
     1201 tctttttccg gaaaaacaaa caacagcacg aggttacctt cctgtagact gggaatatgt
     1261 tcataaagaa ctaatgaaaa agaatatgac attgaaactc cttcatagag aatatggggt
     1321 acatggaaca tagaaacgaa aatgcacggt agaaaagcga atgaatatag tataaattct
     1381 acaaatcatt cgctttcttt attttatata tggttggtcg agagagacca ttttctttgg
     1441 caatttctgc aactggaact ccttgattta acatgtccac cacagtttta aagactaatc
     1501 gtttttgcgg atctgttgag ttctcagaat attcaattgg tcgcccctta tatcgaccag
     1561 cttttttggc caataatact ccttgccgct gacgttcacg gattttctca cgttcgtttt
     1621 gtgcaatata acttagcaat gataaaaaca tatcgaacat agcagtatct aggagttcat
     1681 ttccagtatt aaaatctaaa aactgtgcat ctagtatttt taatgctact tttttctgtt
     1741 tcataaaggt aactgtattt tttatgtctt cataatctct gcctaggcga tccagtgatg
     1801 caacaatgac acaatcacct tctcggatat aatttagtaa tttttgaaac tcaggtcgct
     1861 ggatatcttt accactggct ttttcttgga ataatttatc aatgttaagg tcttccatca
     1921 ttgccaattg tcgatctaaa ttttgatctt gtgaagacac tctaatatat ccaatttttg
     1981 ccataaaaac tccttttaaa aaatgatata atttacatgt aaaatttact tgtagtttta
     2041 ctgttatcat agcattttta aaaataaatt ttaagtctac ttataattta ctttggaggt
     2101 tttgaatgaa tgaaattaag cgcaataaaa attcttttgg tgtttattta ggagagccta
     2161 gctcggaaca gttaaataaa ggtgaagctc gtcatgctct atgtcgaaat atattttatg
     2221 gtagaaaagg caaactttat caaacttatt acgacggtat ggaggaacag ctcaacgcat
     2281 taggattagt aactaatgct atcatatatt ggaattcact atatttagaa aaagtctttg
     2341 aacaaattaa aacggaaggg ttcgactgtt ccgaagaagt gttacgcaag caatttccat
     2401 taattagtga tcacattaat tttgttggca aatatagttt ccgctatgaa aaagaattat
     2461 cgaatggaat actacgtccg ttaaatatag agaattattg agaatgttcg tcttttggtt
     2521 cctaccgtgc attttcgcac ggatgttcca gatactattt ttgaatgtat aaaattgaaa
     2581 acacaagaat actaataaat caacgattct tgtgttttta atttttcact accgtgcatt
     2641 ttcgtttcta tgttccatgt accccaatct tgaaaaatta attaaagact ta
//
LOCUS       DAMEET010000158         2395 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.166, whole genome shotgun sequence.
ACCESSION   DAMEET010000158 DAMEET010000000
VERSION     DAMEET010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.166"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..93)
                     /locus_tag="PB780_002577"
     CDS             complement(<1..93)
                     /locus_tag="PB780_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDE0734581.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREV"
     gene            complement(167..448)
                     /locus_tag="PB780_002578"
                     /pseudo
     CDS             complement(167..448)
                     /locus_tag="PB780_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
     gene            910..2292
                     /locus_tag="PB780_002579"
                     /pseudo
     CDS             910..2292
                     /locus_tag="PB780_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          872 a          409 c          399 g          715 t
ORIGIN      
        1 aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa taatgacgtc
       61 ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt cttttttctc
      121 aattttacac taaaatagat tttttggaaa actttgcaac agaaccgaaa tgagtatagt
      181 atgaattagt aatttggttt tcatctaagt ttagctttga tttactaaaa ttgatatctc
      241 gatacatgtt caatacatct aatacaaatc gagaatcttc tttcggaaat ggctcagaaa
      301 gatcactcca aatttcatca tcataatgat actcgtaacc ttctctcaat atttcgattt
      361 ttctttcata ataagatctg ttttggtcct caaatttagc caataactca aattgattta
      421 ctagtgacaa tctctgaaaa ctgtccatta aatgtttctt cctatcttgt tcattaacaa
      481 aaaattataa gcccttacaa cgaatattat atattaaatt taattgtagg tgaacacgaa
      541 ttttataaat atttatggtg ttaatgacaa atgatatccg actcctcttc atacgttctg
      601 gactatttgc taatagatta aaatttcagc atctatttct gaccataaat aaatgagctg
      661 ccgaaaagct gccaaatcag acttgaaaat aaaggatcaa aaacttttaa gtttctgacc
      721 ccgtaactca cacttcaaaa tcttacttaa aaaggagtct gaaagaaaaa aagagtattc
      781 aaaatttttt tctaactagc tacatcacat ctatgcattg acactcaaag aaccagaata
      841 agtcatcaga aagcaagggg gtgcggacat aatcctggac gaaaaaataa acttaggagg
      901 aatgtccgtg tgttttccca caaagagcgt gtaagggcaa ttcagttatt tttaaaatac
      961 gattgctctt atgctgcaac cattcgagaa ttagggtatc cgtcgattgg tgcactacgg
     1021 aaatggtata aagagtattt ggaatccgga gagctccatc aggagcaccg aaaaaaatca
     1081 aagtattctg aagaacaaaa acgaatagcg gtgaatcatt attttgaata tggtcaatgt
     1141 tatgcacgaa ccattcgcat gcttggttat cctaatagag agtccttgcg gcagcggtgt
     1201 gaagaacttg caccaggagc tagaaaactc cgaaaaagtg cggtaaagtt aacacaagat
     1261 caaaaagaag ctgtgttgaa aaaattctat aaaccgcaaa ctaataggaa aaatttagcc
     1321 gaatctggag gaattagtag agttactcta tatcaatgga aaaacacggt tcttggaaag
     1381 gattttccat tgtgtatgac tttaaaaaat caggaaaaac aaaaagagct acttttaaag
     1441 gaagtagaag acttacaaaa acaagtacac caattacagc tcgaaaaagc attactcgaa
     1501 ggagcggctg aactgctaaa aaaagaaaaa ggcgtcaatc ttctttgtct atcaaatcaa
     1561 gaaaaaacga tattgattga tgccttacga aatcagttca ccttgaaaga gcttcttcaa
     1621 cagctccagt ttcctaaaag cagctatttt tatcaaaaac aagcacttga gaaaccagat
     1681 aagtattaca aagaacgtca aattaattat cacgattttc aatcataact tctgtgcgta
     1741 tggttaccgt agaattcacc aagctttaaa aaatatgggt aaaaagcttt ctgaaaaagt
     1801 tgtacgtaga ttgatgactg aggaaaatct atttgttaaa ttttctagac gaaaaaagta
     1861 ttcttcgtat gctggggaaa tttctcctgc acaccccaat ttattaaatc ggaattttaa
     1921 agcaaagaag ccaaatgaaa aatggttaac agatattact gaatttagaa ttctcgcagg
     1981 gaaaatctac ttatcaccac ttgtcgattg ttacgatggt gctattgtta gttggactat
     2041 tggaacaaag ccaacttgct tcactctatg tctaaaaaga gttattcacc agataattca
     2101 gcgtgcgaag gtttctttgg acgattaaaa aacgagatgt tttatcaacg tgactggaag
     2161 aatacaacaa tcaatcagtt tataaacaaa ttggatgatt atattcattg gtataataat
     2221 caacgaatta agttatcttt aggaaggcta agccctcttc aatatcggaa gaagcaaaga
     2281 tatatttgtt aaaaaggtcc aaaaaaaata tccgcacccc ctttttttgt aaaaaactat
     2341 tgcacaatac aagtagccta cggctatcga aatttccata actttcgagc aagaa
//
LOCUS       DAMEET010000159         2395 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.167, whole genome shotgun sequence.
ACCESSION   DAMEET010000159 DAMEET010000000
VERSION     DAMEET010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.167"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            316..570
                     /locus_tag="PB780_002580"
     CDS             316..570
                     /locus_tag="PB780_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDE0734582.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="PB780_002581"
     CDS             571..888
                     /locus_tag="PB780_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDE0734583.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="PB780_002582"
     CDS             1447..2382
                     /locus_tag="PB780_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734584.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          861 a          339 c          453 g          742 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagag
//
LOCUS       DAMEET010000160         2249 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.169, whole genome shotgun sequence.
ACCESSION   DAMEET010000160 DAMEET010000000
VERSION     DAMEET010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.169"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(148..2145)
                     /locus_tag="PB780_002583"
     CDS             complement(148..2145)
                     /locus_tag="PB780_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDE0734585.1"
                     /translation="MIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKA
                     LVTNKANQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHL
                     KEATKRLSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVY
                     IKNSGVTDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADE
                     AEKYLAKGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGS
                     NLMLSMNAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHE
                     QGSKEITENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKS
                     SVFNRSGQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRD
                     SFKEFGLGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYT
                     PIQLAQYASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMA
                     ILREGFHQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGP
                     TDNPEIAISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          572 a          504 c          411 g          762 t
ORIGIN      
        1 ttttgtcaca accttttttg ccgagtaaac ggtgttcaaa agccagtttc tcggtatgaa
       61 gtcaaattta agcaaaaatg tgagaagcta ttttcacaaa atttttcttc atactcgaaa
      121 ctaagcggct ttttcacgac ctctttttta ttttgccatg aacatatcat aatacgcgtc
      181 cattatttct ttagcaatcg tcaagtttat gtggtcttgt tcgtctaata agttcggcaa
      241 gacaacactg atggcaattt ccggattgtc tgttggtccg tatgccacga tattactatt
      301 gactgtcgca atatctggat gaccttccgc aaccgtttct gcagtccctg tttttgcgga
      361 aaggtccatt ttcgcactgg ctaatggttt ggctgttgtg taaggatcgg taccatggac
      421 aacttggtgg aatccttcac ggaggatcgc catattttct gcagaaatat tgaccgtatt
      481 ttctactgtt gtgccaattt ctttcttcac ttcacccaag tttccatttt cgtcattgcc
      541 atagatcgct ttgactaggt gcggctgtac tcgtttcccg ccattagcga ctgttgaagc
      601 atattgagct aattggattg gtgtatatgt gtcaaactgt ccaaatgcca aatcggtaaa
      661 gttacctgga gcataccatt ctttgccatt atccgcattg aacttcttca tataatcaac
      721 agaacgtgag atcccaggag attcattcgg caaatcgatc ccagtcgttg taccaagacc
      781 aaactctttg aaagagtctc tcaattgctg ataagcttta gcttcttcat ctaaactagg
      841 tagtcccatt ccaggtgtat agtctagtcc taacatcttt aacgccactt tgatcatata
      901 cgtattagaa gacaattccg atgctttgac tgcatcaaga gcaacttgtc cgcttcgatt
      961 aaagacagag cttttttctg aagcaccttg cagacgaatc ggctcatcaa tcaaaacttg
     1021 atttccgctg atcgcgttac ttgcccagcc tgctgtcaaa gtaccagcct tgactacaga
     1081 ccctggatca aaggcgctag taatcgtgcc taaagcattc tcggtgattt ctttggaacc
     1141 ttgttcatga ctgaaaccag tcattgctag cacttctcct gtctgaggat tcatagccac
     1201 tgcataagca ccaggcgaat actgtgcttt gccgttgttg atcaatgttt gataattcct
     1261 tttcaaaatt tcttctactt ttgattggaa ttctgcattc atagataaca ttaaattaga
     1321 acctttttcc ccagagaaga tttctttttg attcgaaaca tttccttcat tatcaaggga
     1381 aacttcatat tgggttttgg ttccttgcag gacatcttca tattgttttt ccaaataact
     1441 ttgtcctaca cggtcattcc gagaataacc ttttgctagg tatttctctg cttcatctgc
     1501 tggaagtcct tgcttttcag tcgtcacact gccaagaata cttttcagtg aacttgctgc
     1561 attatattct cgtgtccagt ctgtgccagt cgaaactcct ggaagttcag aagcgcgttc
     1621 tgccacaaca gctaactctt gatcagtaac accagagttt ttgatataga cagtgtttaa
     1681 ttcatatgca ctattcatcc gtttaaagat ggtagcaatt tttaattgct cctcgtttaa
     1741 attaatctca tcagctgtga ctttattgac catcgcacta tattgttcgc tgttccctaa
     1801 ttgcttttct tttgccgaaa gtctttttgt ggcttctttc aaatgtttgg gatcagccag
     1861 ccaaaaatct tttttatctc tatctgtcag attttcatct accggaacat cgatcagcga
     1921 gttcagcttc gttgcgattt caagcaaatc tttagccgta acttgtgttc ctctcgtata
     1981 tgtgatggcc tgattcgctt tattcgtaac taaagctttt cctgtggagt cgtagatcat
     2041 tccgcgagga gtactttctt ggaccgtaat cgtagaagta gacttcactc gcgcctccat
     2101 ctcttcgccg ttcacgatct gcaaataacc caatctagca atcaacgcaa caaataacgt
     2161 aaatataatg aaaaaaagaa aatttaagcg aaaaggaaca tgggaccggc gaaacgtttt
     2221 gttcttaggt tcttgtgcac cttttctta
//
LOCUS       DAMEET010000161         2220 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.170, whole genome shotgun sequence.
ACCESSION   DAMEET010000161 DAMEET010000000
VERSION     DAMEET010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.170"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(51..1598)
                     /locus_tag="PB780_002584"
     CDS             complement(51..1598)
                     /locus_tag="PB780_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDE0734586.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="PB780_002585"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="PB780_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDE0734587.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2043..>2220)
                     /locus_tag="PB780_002586"
     CDS             complement(2043..>2220)
                     /locus_tag="PB780_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734588.1"
                     /translation="IVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNGINNY
                     ILQAILKELLSNAL"
BASE COUNT          622 a          465 c          397 g          736 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaatc
//
LOCUS       DAMEET010000162         2179 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.171, whole genome shotgun sequence.
ACCESSION   DAMEET010000162 DAMEET010000000
VERSION     DAMEET010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.171"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            134..307
                     /locus_tag="PB780_002587"
     CDS             134..307
                     /locus_tag="PB780_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734589.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            947..1420
                     /locus_tag="PB780_002588"
     CDS             947..1420
                     /locus_tag="PB780_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDE0734590.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1429..1656
                     /locus_tag="PB780_002589"
     CDS             1429..1656
                     /locus_tag="PB780_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDE0734591.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          707 a          345 c          321 g          806 t
ORIGIN      
        1 ttcggagaat taaaaaatac aataaataac tttagttact ttaatgcgtt cttactttat
       61 ttttatatta tcatttttct tattcacttc tttggtttat actattctta taaataaaaa
      121 cgaggtatgt cctatgatga aaagattgta ctattcattg atcataacga ttggctattt
      181 gattgtatca aatctaggaa atatggtatt tggtatcagt aaagaattca gttggactac
      241 tacattatgg gaatctctat tctttttcat cttcgtattt ttgttacaaa attatcgaaa
      301 aaaataaaca tgtgccatct atctgttggc acatgtttta ggatttatgg tactttttat
      361 tcggcaatcc attttatttt tctacttctt cttaatacct ctaacacttt tgatttataa
      421 actaatccaa tgcatctact tgcgcttatt aagctatttc agctagatag taccacccca
      481 tgcgtcaatg cttgattttc cttttgtata acattgtata agcaataaag gacaaataag
      541 tggtgattac accaaaccat tgaataagaa aaattctgat acgtaaataa acatgaactt
      601 gaaaaaatta atgaatcggt tagctactac gtagttgtct tttttctcat acagttctgt
      661 taacttacac ttctcttcgc tagataataa aaaatcctcc aactgtattg tccaatataa
      721 tcccaggcat ctaaactagc attatagaag agacacacat agaatctaaa ataactggtt
      781 ttaaatgttt cttacaatca aaaaaagctg ttaaatgcac tccacacctt catactctat
      841 cattttttat aaacgatata tttttattgt ttttcgataa aaaatagttg ttttacaata
      901 attattctgc tatattttag ctatcaaaga taaaagaggg ataaaaatga aatttaaaac
      961 attgctatta aaatttgtcg ttttaggtat aagtgccgta tttattttgt tcggcgtact
     1021 tttttatact cagatcgcta caagcgaaaa aggattcaat tttgattggc agaccacatt
     1081 atttgtttct gtcatttact ttgcccttct cctagggatc gttattgctt atttcttatt
     1141 gaaactattg aaaaaaatcg aagataacca agctttttcc actcaaagct taaacatact
     1201 aaaaaaaatc gaacgaacca ttttgtttat tggtttcgcc ttttttggct tacttcctaa
     1261 atttatgaaa gtagcggatt tagacgatgc tccaggtttg atgttggtag gaatcgctgt
     1321 cgtattcatt ccttttgcag tatacacatt aacaagcgtg ctggaaaaac tgcttgaaca
     1381 tgcgatttta cttaagacag atcatgattt gacagtgtga ggtgcgacat ggcaattatc
     1441 gttaatttag atgtagtatt agctcaaaaa aaaatgagtg tgactgaatt gagtgagaaa
     1501 gtgggcatta cgatggcaaa catctctatt ctaaaaaatg gaaaagcaaa agccatccgt
     1561 ttttccactc tcgaaaaaat ctgccaagtt ttggagtgtc aaccaggtga tttgttgacc
     1621 tataaaaatg actcgaatac tataccagaa gaataaaata aagtagaaca aacagtaaat
     1681 aaaaaataat ctgaatgata gatgtgtgag acttttagaa gttatttacg tctattattc
     1741 agatttttta tttcgattga ataccgtttt atctataaat aagatatgta ctgactcata
     1801 caaagggggc cttttcttct ccatgtacta tcacttttcc tgatacaact ggtttcctgt
     1861 tacgttgtag ttggatctgc ttttagtata atactttatt cattccacca gactgatagc
     1921 agtttgagca gtctaaataa tcatgtagta ttacaggcca ttctgtttca taccttgaag
     1981 tgcttaaaac ttcagctaat ggcccattgt gttaactctt actccattct tttttagtaa
     2041 gctcccatat atactgtaaa gccttctata tattatgtca tgttgctttt taacatttac
     2101 tatgtagttt tgataaaatg atttgaataa cagtgatgga caattctttt ttatttttaa
     2161 cattattttg ataataatg
//
LOCUS       DAMEET010000163         2095 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.172, whole genome shotgun sequence.
ACCESSION   DAMEET010000163 DAMEET010000000
VERSION     DAMEET010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.172"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            472..1089
                     /locus_tag="PB780_002590"
     CDS             472..1089
                     /locus_tag="PB780_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDE0734592.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            1130..1429
                     /locus_tag="PB780_002591"
     CDS             1130..1429
                     /locus_tag="PB780_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HDE0734593.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1463..1630
                     /locus_tag="PB780_002592"
     CDS             1463..1630
                     /locus_tag="PB780_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDE0734594.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1675..>2095)
                     /locus_tag="PB780_002593"
     CDS             complement(1675..>2095)
                     /locus_tag="PB780_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDE0734595.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          723 a          322 c          404 g          646 t
ORIGIN      
        1 ccaaatgaaa tgtggtttga agttacagtt gaatcaagcg acttcaaaga aaaaaagaaa
       61 gaatacaatt atattgtaaa ggcaaaagta tcaaaaagat ttatttttaa ccctaaatta
      121 gaaatatata gtgttgtaat agaataatag tgttaaaaaa attgtaaaag ttaaagaagt
      181 caaattctga tgtgaaaaat aaaatatacc atggaagaca taaaagaaaa actggataac
      241 tagtttattt aatggcagac cgttaaggcc tgccattttt atgtatttta gcggaataaa
      301 tataatcatg cgggagtgta aacttactgt ttcaaaaagt atactttttg acatgccttg
      361 attttctttg tttatgtatg gcataataac cacataatac aatttgaaac gtttctgatt
      421 taatctgatt gttttgaaac aattttagtg aaaaaagggt agataaaaac gatgagaaaa
      481 tttttttata tccgggtcag tagttatgat caatcgatcg aacgacaact gatttccgcg
      541 aaggaattag gaatccctga agaatatata tttgtagaaa aagccagcgg caaagatttt
      601 aaacgccctg aatatcaatt aatgaaacgg atgtttcgaa aaggagattt actctatcta
      661 caatcattag atagactagg cagaaataaa caaatgattt tagaagaatg gcgagaatta
      721 actaaggaaa agcagattga tatttctgtg ttagacatgc ctctgttaga tactgctagt
      781 tacaagcaac tagatgggtt agagacgctt atctctgact taattctgca attactcagc
      841 tatatggccg aagacgagcg gaaacgaatt aatagccggc aaagagaagg cgtagaagct
      901 gcgaaacaaa agggtgtaaa attcggcaga aagaaaattg ctttacctcc tgaatttcct
      961 gcgatctatt ctgactggaa agcaggaaag ataactgcag tctatgctat ggatcaattg
     1021 ggcatgaaac gaaatacctt ttatcgacgg gtgaaagaat atgaacatac cttgcaccac
     1081 ttttcttgat agatataaca gctattagac gggaagggag agtttgaaaa tggacttaac
     1141 gaatcaaagg gtcgttttta ctggaacgct gcagcaaatg acacgaaccc aagccatcgg
     1201 tttggttcac tctttaaacg gccattgtca atcaagtgtt acttcaaaaa caaatttcct
     1261 ggtttgtggt cagtattcta agtcgttgtt tgatgattct ctctatacaa aaaaggaaca
     1321 aaccgcgcgg gacttgattg cgttaggtca tgagattacc attttatcag aagtcgaatt
     1381 ttatgcgtta attagtcaac agttgaaaga atggcaacgg tatttataat cgttagtaaa
     1441 atttacaagg aggaaaattt ttatgaagcg aaaatcttat gatcgttggg gcattgaaat
     1501 aactagtttt aatcgaaata ttgcttttca cgtggagtgt gactgtgggg aattagcaaa
     1561 aagtgtttat caaaaaaatt acttttactg tcctaaatgc catcgccgat acggggtaca
     1621 tggaacatag aaacgaaata gtgcggtaga aaagcgaatg aatgtagtat atttctacaa
     1681 atcattcgct ttctttattt tataaatagt tggtcgagag atcccgtttt cttttgcaat
     1741 ctctgcaact ggaatgcctt gctttaacat gtccaccacg gttttaaaaa ctaagcgttt
     1801 ttgcggatct gttgagttaa tagaatactc gattggtcgt cccttatatc ggccggcttt
     1861 ttgagccaat aatactcctt gccgctgacg ttcacgaatt ttctcacgct cgttttgtgc
     1921 aatataactt agcagtgata aaaacatatc gaacatagcg gtatccagga gttcatttcc
     1981 agtattaaaa tctaaaaact gtgcatctaa tatttttaac gctacttttt tctgtttcat
     2041 aaaagcaact gtatttttta tatcttcata atctcttcct aaacgatcta gtgat
//
LOCUS       DAMEET010000164         2043 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.173, whole genome shotgun sequence.
ACCESSION   DAMEET010000164 DAMEET010000000
VERSION     DAMEET010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.173"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            307..1092
                     /locus_tag="PB780_002594"
     CDS             307..1092
                     /locus_tag="PB780_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDE0734596.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            1105..1956
                     /locus_tag="PB780_002595"
     CDS             1105..1956
                     /locus_tag="PB780_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HDE0734597.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
BASE COUNT          662 a          336 c          449 g          596 t
ORIGIN      
        1 aaagtcttgt cacagccttt ttttccaaat aaatggtttt tcagaagtag cttcttcgga
       61 aataagccga aatttcacaa aaatttgaga agtaattttt gtaaattact tcttatttct
      121 tgaagctggc cacttctgtc acaacctctt tctttataga aataaaagga cactagcaat
      181 tcatgaaaac gattgctaga gcgtatcatt tgctttagac tatatatctt ggtgtacact
      241 cactttaaga cggaaataaa agttatccgt aataacaggt ttaggtatga aaaaggagtg
      301 ggtactatgt ataaggaatt agaaaacaaa gtcgcagtag taactggtgg ctcaaagggg
      361 attggaacag cgattgctca acgattcggg aaagaaaaaa tgaatgtagt tgtcaactat
      421 aactcggata aagaaggagc agaaaaagca gtcaaagcaa tcgaagaggc aggcggaaaa
      481 gcagtctctg tacaagcaga tgtcagttct gaagaaggaa tacagcgttt acttgatacg
      541 gctttagaaa attttgggac actggacgtt tggattaata atgctgggat ggaaaatcag
      601 catccaaccc atgaacttag ccttgaagat tgggaaagag tattaaaagt caatttgact
      661 ggcgttttta tcggaacgaa agcggcttta aattacttta cagagaatga taaaaaagga
      721 aatatcatca atatgtcttc tgttcatgaa caaatcccat ggccaacgtt tgctcattac
      781 gctgcttcaa aaggcggagt aaaactattc accgaaacgg tagcaatgga gtacgcacca
      841 aagggaatca gaatcaatag tataggacct ggtgctatcc gtacacctat taatgcggca
      901 aaatttgatg atcctgaaca aaaagaactc ttggaaagta tgatcccact aggacgcgta
      961 ggtgaaccaa aagaaatagc ggcgacagct gcatggttgg cttcagacga atccagttat
     1021 gttacaggta ttacactatt tgcagatggt gggatgactt tatatccttc tttccaaggt
     1081 ggaaaaggat aagggtgaat aaaaatgggt ttattgattg cattgatccc ggcagttggg
     1141 tgggggatcc agccactgat tttgaaaaag attggtggca gaccaacaaa tgagatatta
     1201 ggaactggta ttggagccgt gctcgttggc ttacttgttc aattattcgc ttctccagct
     1261 ggtatttcag tgactacatt tttgattagt ttactttctg gtgcattctg ggtcatcgga
     1321 caaacaggac agtatacatc tttcaacttg attggtgttt ctaaaacgat gccaatttcg
     1381 actgcattac agttagtcgg aacctcattg attggtgtct tcgcttttgg agaatggtct
     1441 ggaagtatgg gaaaaatcat tggtgcagca gcaatcattc ttcttgtatt tggttctgct
     1501 ttaacggcaa tcagtgatga tcctggaaaa aaaggcggta tcacaaaagg aatcagtcta
     1561 ttagcatcaa caagtattgg ttattgggtt tacagtgcat tgcccaaact agttgatgca
     1621 agcggacttt ccattttctt tccgcaaatg atcggtgtct ttttaggggc agtgatttat
     1681 gtgattttcc gaaaaccaac cgcattttct gaagacaaga gttggaaagc aacaatagtt
     1741 ggagccatct tcagtgtgtc agcattggct tatatttttt cagcaaatga aaatggggtt
     1801 gctactgcat atatcattac acagctaaac gtagttatct ctacattagg cggtatgtac
     1861 atcctgcatg aaaataagac accaaaagaa aaaaaattaa cgattatcgg attattgctg
     1921 atcgtagtag gaagcatggt cacggtcttt atttgaggat aaagatggta aaatctcatt
     1981 tattaaaaaa atatttgttt gaaccaaaca gtaagtactt ataaaaaaat agcgctagaa
     2041 ttt
//
LOCUS       DAMEET010000165         2003 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.174, whole genome shotgun sequence.
ACCESSION   DAMEET010000165 DAMEET010000000
VERSION     DAMEET010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.174"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..378
                     /locus_tag="PB780_002596"
     CDS             <1..378
                     /locus_tag="PB780_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDE0734598.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="PB780_002597"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="PB780_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDE0734599.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="PB780_002598"
     CDS             1859..>2003
                     /locus_tag="PB780_002598"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734600.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DAMEET010000166         1996 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.175, whole genome shotgun sequence.
ACCESSION   DAMEET010000166 DAMEET010000000
VERSION     DAMEET010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.175"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..742)
                     /locus_tag="PB780_002599"
     CDS             complement(<1..742)
                     /locus_tag="PB780_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HDE0734601.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGICYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEA"
     gene            complement(746..1804)
                     /locus_tag="PB780_002600"
     CDS             complement(746..1804)
                     /locus_tag="PB780_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDE0734602.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT          641 a          425 c          328 g          602 t
ORIGIN      
        1 tagcttcatt atcttgaagc tctccacggg ctgtattgat caagatgctg ccttttttca
       61 tttgagctaa aaatccttca ttgatcattt gatcgttttc acctgggata tgaggtacat
      121 gaatactgac gatatcgctt ttggctaata actcttcaag cgagtggaac gtcaatactt
      181 ttttagcagc ttctgtctga tgaatatcgt agccgataac tttggctcct agtcccttaa
      241 acaaaacagc ttccgtcaac cctatcctgc ctgttcctat gatccctact gtgcaatttc
      301 tgatttcacg actaaacatt tgctcatcca ctcgaaaatc tttgaaagat gttcggatcg
      361 tcgtatacgc agtatttctc aaaagcatca tggcaaaagt caaagataac tctgctatcg
      421 catttggcga atacgatggt acacgggcta ctgtcatacc aaaatcagct gccgcttgta
      481 gatcgatatg attgactccc acagtccgtg taaaaacata acaaatccca tattcctgca
      541 tttttgcaag attggtctca tctgctatac aatttcccct caacaataca gcatcatggt
      601 cctttgctga cacgatattg tcatgtgtca acagttcttc ataaagactg agatcataat
      661 ggtaaatatt caatttttca aaaaaatcta tctcgttcgg acgaacaccg taacatgcga
      721 ttctcatact ttcttcctcc atttcttatg aaaataaacc tatgccatga atcatttgta
      781 atacaacgat ccatacaggt gtcagtacca cagcaaaaat agttgacagt aaggatgcat
      841 tggaggctaa aagtgcctcc ctatcaaaac tgatagcata ggctgctgcc actgttgcag
      901 ctggtgtagc catcatgata acgattgttg ccagtgcttc aaaattgact ggtagaatat
      961 tcgttgccgt caaaactgct agaatcacga tattcaatgc aggtacaaat accactttga
     1021 ctagactgta ataccatgaa gttttttcag atgcagcttt tttgaagctt acttcaccta
     1081 atgttgcgcc gattgctagc cacgccaatg gtgaacttaa accagctaaa taatccatcg
     1141 gctgaaatag ccacggtgca gtctggtcaa ttcgcaaaaa cgctacttgg cttatctctc
     1201 cttctgcacc tgtaactgac acttgtggca gatacccttg gaacagccaa atgaaaaggc
     1261 cggcaaaagt agcgataatg attggattga cgaacatcgc tttgatattt ttagcttcca
     1321 tcttcaagcc actcattttt atgtatccat aagaatacaa aaagattcgg tagccaatat
     1381 tgaaaattga ggaatacatc acgccgacag gaccataaat cgcactgacg ataggcgttc
     1441 cgaaaaaggt cgtagaacca aagatcgtca ggacacgcaa cgttgtttgc ttatcccctt
     1501 tatagcttaa gaaaaaaggt tttgaaacta gaataagaat aatataaata actaatcccc
     1561 aaatcaagac attgattcct tgcatcagta attctgtatt gatatcttgc atgaaagctc
     1621 gaaatgccag tgctggcaaa gccactgtta agacgacttt cgatagaatt tttccaacag
     1681 attcagaaaa aatccccttt tttctacaaa caaaccctaa taatataata aaaactgttg
     1741 aagtgattgc actgacaatc gacatatctg tcaaagtcga tcgaaaaatg tcaaataagt
     1801 tcatctttct cctcctaatt atgttcattt attcacaata aaaaaacgct tttattttgc
     1861 taatacgtat taacattttt aattttatct ttacattgtt acttattgaa caataggaaa
     1921 ttaaagaaac ttttgaaact tatgtaaagt gatttttact agaacacaaa aaaaacctta
     1981 aaaccaaact taagaa
//
LOCUS       DAMEET010000167         1994 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.176, whole genome shotgun sequence.
ACCESSION   DAMEET010000167 DAMEET010000000
VERSION     DAMEET010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.176"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            127..1851
                     /locus_tag="PB780_002601"
     CDS             127..1851
                     /locus_tag="PB780_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDE0734603.1"
                     /translation="MKKKLLTLFLVMSFSIVLSVYYNTIVFSEQINYTEEQYNIEKEK
                     ALKVGYSEEQFKQIMEIPTNLSNENSETRIVNYTMTSNQTKVINKAMEQIGKPYEWGA
                     SGPNSFDCGGLVKYVYKQAVNIELPMGTTNQEQYGTEVSLNSLKPGDLLFYGNRGATY
                     HVGIYKGNGVMIHAPQPGETVKEVNIQYFYPNFAKRILPDEPDYPYIDYNKMVTVTKA
                     WSIWNDLQFSHEIKKAIIGDNYKIGKVYTNPENNNKYGEILVSNKVYGYINFYAVKEL
                     TSVQINRYLTSKDSGQPTWGNLECTISKGQTTKDKIYFVKGAYNLGDGKYLYSIYKDQ
                     DSSEWLGYLKAHVSLAYTPIEEINKNVTVTKNWSIWNNLQWEKEIEKPQIGSVFSARL
                     KLTNVSNNAVYYKLYKSGKFYGYINAEAVKDLTTTKLNKYVTFSVNNEDFWSSLDVNY
                     SKGKTERGRVFYISISYNTADNQPIYSVYTDETCTEWRGYYKGNNFEDTQITMLENKS
                     VKVTKSGYTVWGDLNFWTKSGISNTGDIYTTDRKFYNFTNNAYYYELKKDGRVYGYIN
                     SEAAVEMN"
BASE COUNT          783 a          241 c          348 g          622 t
ORIGIN      
        1 agtttgtaac aaacatatat tttttatatt acaatatttc aacaaattaa aaaaaattat
       61 atcttcttaa acaatgataa tattaaaatg caagcgcttg taaaaaataa ggagaagagg
      121 taaaaaatga agaaaaaact attaacttta tttcttgtga tgtctttctc aatcgttttg
      181 agtgtgtact acaatactat tgttttttca gaacaaataa actacacaga agaacagtat
      241 aatatagaaa aagaaaaagc tttaaaagtt ggttatagtg aagaacagtt taagcagatt
      301 atggaaatac ctacaaattt atcaaatgaa aatagtgaaa caagaatagt aaattatact
      361 atgacttcta atcaaaccaa agtcattaat aaagcaatgg agcaaattgg aaagccatac
      421 gaatggggag catcgggacc aaattctttt gactgtggtg gtttagtgaa atatgtttac
      481 aaacaagctg taaatattga actgcctatg ggaaccacca atcaagagca atacggtaca
      541 gaggtaagtt taaattcact taagcctggt gatttattat tttacggcaa tcgaggtgca
      601 acttatcatg taggtatata taaaggtaac ggtgtaatga ttcatgcacc gcaacctgga
      661 gaaactgtaa aagaggttaa catacaatat ttttatccta actttgcaaa aagaattttg
      721 cctgatgaac ctgactaccc ttatattgac tataataaaa tggtaactgt tacaaaagct
      781 tggtcaatat ggaatgattt acaatttagt catgaaatta agaaagctat tattggagat
      841 aattacaaga tagggaaggt ttatactaat ccagaaaata ataataaata tggagaaata
      901 ttagtaagta ataaagtata tggatacatt aatttttatg ctgttaaaga gttgacctct
      961 gttcaaataa acagatattt aacttctaaa gatagtggtc aaccaacttg gggaaatcta
     1021 gagtgtacta taagtaaagg acaaactaca aaagataaaa tatattttgt taaaggagca
     1081 tataatttag gggatggaaa gtatctatat tctatttata aagatcagga ttcctctgaa
     1141 tggttagggt atttaaaagc acatgttagt ttagcctata ctcctattga agaaattaat
     1201 aaaaacgtaa ctgttactaa aaattggtcg atttggaata atctacaatg ggaaaaggaa
     1261 attgaaaagc ctcagattgg ttctgtattt tcagctagat taaagttaac caatgtatcg
     1321 aataatgctg tctattacaa gctttataaa tctggaaaat tttatggtta tatcaatgct
     1381 gaggctgtca aagaccttac aacaacaaag cttaataagt atgttacatt tagtgttaat
     1441 aatgaagatt tttggtcttc tttagatgta aattattcaa aaggaaaaac tgaaagaggc
     1501 cgagtatttt acattagtat ttcatataat accgcagata accaaccgat ttattcagta
     1561 tatacagatg aaacatgtac ggaatggcga gggtattata agggaaacaa ctttgaagac
     1621 actcaaatta ctatgcttga gaataaaagc gtgaaagtaa caaaaagtgg gtatacagta
     1681 tggggagact tgaatttttg gactaagtca ggaatatcga acacaggaga tatatacacc
     1741 actgaccgaa aattttataa ttttaccaac aatgcttact attatgaatt gaaaaaagat
     1801 gggagggtct atggctatat aaatagtgaa gcagctgtag agatgaacta aaatattgct
     1861 gtcccttaat cttatggggc agcaatattt ttatattatt aatcattttt ctgaagcgag
     1921 ggtagaagaa ttaatcaata gtttttcttg ttttgtaata taatagttta attaccaaaa
     1981 attatttaag taaa
//
LOCUS       DAMEET010000168         1990 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.177, whole genome shotgun sequence.
ACCESSION   DAMEET010000168 DAMEET010000000
VERSION     DAMEET010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.177"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            17..940
                     /gene="truB"
                     /locus_tag="PB780_002602"
     CDS             17..940
                     /gene="truB"
                     /locus_tag="PB780_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDE0734604.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            944..1885
                     /gene="ribF"
                     /locus_tag="PB780_002603"
     CDS             944..1885
                     /gene="ribF"
                     /locus_tag="PB780_002603"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HDE0734605.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKKDEQATAEYFSQQ"
BASE COUNT          705 a          324 c          448 g          513 t
ORIGIN      
        1 gaaatggaga aaagacatgg acgggttatt acctttatgg aaagaacgcg gaatgacaag
       61 tcatgattgc gtattcaaat taagaaaaat cttacatacg aaaaaaatcg gacatggggg
      121 tacgcttgac ccagacgtag acggtgtgtt gcctatttgt attggaaaag ccacaaaagt
      181 tatcgagtat ttgacagatt ctgggaaaat ctatgtaggc gagataactc taggtttcag
      241 tacaacgacg gaagatcgat ccggagaacg ggtagaaaca aaaccagtag atatgtcttt
      301 atctgaagaa aaaatagatg aagtgatggc ttcatttatt ggtgagatca cccagatacc
      361 accaatgtac tcagcggtaa aagtcaacgg taagcggtta tatgaatatg cccgcaataa
      421 tgaaacagtg gagcgtcctg ttagaaaggc tcagatttac cgttttatcc ggacaagtga
      481 attgacaaga gacgaagtga caggaaccat ttcttggcgc ttcgaagtag aatgcgggaa
      541 aggaacctat gtccgcacat tagctgtaga tacaggcaag aaacttggtt accctgcgca
      601 catgtctgat ctgacccgaa cagcaagtgc cggtatgtcc cagaaagatg ctctgacatt
      661 agaagaagta gctcaagctg tgaaaaatgg gacaatcgat gattgtttgc tgccaataga
      721 aaaaggagtg aaacgattca aacgagtaga tattactgat gaagtatggc agaaagtaaa
      781 aaacggtatg agacttgatt atactgtatt tgcgctccca gaaatgccgt cagaagaaat
      841 tgctttgttt tataaaggca aagttgtcag tatctatcag ccaaacccga aagaaaaaaa
      901 caaattaaaa cctagtaaag tattgagaaa tgaggaataa cgaatgaata tcatcaagat
      961 cagacatcct taccaatcag aacaaattcc agaagacgat gtcgttttag tactgggatt
     1021 ttttgacggc gtgcatattg gacaccagaa agtcataaag acaggtatgg aaattgcaca
     1081 aaaagaagga ttgaaattag cattgatgac tttcaatcag catccatcga ttgtctttaa
     1141 aaagatcgat cctagcagcg tgaaatattt gactagcctt aagcaaaaag aagaaaaaat
     1201 ggccgctttg ggtatagatt atttatatga agttgatttt acttcttctt ttgctcattt
     1261 agcaccgcag gaatttgttg atcaatacat cgtagggtta catgcaaaat atgctgtttc
     1321 cggttttgat tatacgtatg gaccaaaaga cattgcagat gtcgctcatt ttcctgaata
     1381 tgccaaagga agattcaaaa tcgtcacagt ttctaaagaa gaagaacaag gtgaaaaaat
     1441 cagctctacc cgtattcgag gattactaga cagcggacaa gtaaaagaag cagcaaaact
     1501 attaggttgc acatatgagg tagaaggggt agttgtccat ggcgatgcac gaggcagact
     1561 actaggattc cctacagcaa atgtcaaagt aaagagcaca gttcatttgc ctaaagaagg
     1621 tgtttatgta tcggaaataa aaattgggga aacttggtat ccagccatgg gttcaattgg
     1681 acataatgat acattcggtg aaggacggga gctgactgta gaattgtata ttttggactt
     1741 taatcaagat atctatggtg aacatgtcgc agtacgctgg aatcaatttt taagagatca
     1801 agtgaaattt tctagtgtag aggaattgat tgaacaactc aaaaaggatg aacaagcaac
     1861 agctgaatat ttttctcagc aataaaacat tatgcataaa atctcttgtt tatgtataac
     1921 tagcaatttc aataaaatgg gtgataaata agcccaggaa aggggctgtg acaaaagtct
     1981 tgtcacagcc
//
LOCUS       DAMEET010000169         1979 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.178, whole genome shotgun sequence.
ACCESSION   DAMEET010000169 DAMEET010000000
VERSION     DAMEET010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.178"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(325..528)
                     /locus_tag="PB780_002604"
     CDS             complement(325..528)
                     /locus_tag="PB780_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734606.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSVKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            complement(728..1744)
                     /locus_tag="PB780_002605"
     CDS             complement(728..1744)
                     /locus_tag="PB780_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDE0734607.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLD
                     VTEK"
BASE COUNT          557 a          459 c          309 g          654 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggttttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cacgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaaca ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaatgct ctccgagggt attcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt catgcaaaat attcgcttat
      661 cgtgaattta cgtcactaaa cattgacgca aatccactct cactcatctg acttttctta
      721 ttcggtatca cttctctgtc acatccaacc ccaaaagcca cgtatccaat tcttttttta
      781 attcgtctgc agattcctta aaagtctctc gtaaatgatt cgttaactca tcattttgac
      841 tttcttgctt cagcaaattc cgatatttat attttgtatg tctagcaatt atcgaccaat
      901 tagaaggctc agctatcaaa ccatcaataa ctattttatc aataggtttt atatcaatcg
      961 gcaaaaaatg cgggcttgca aaactatcta agctttcctg aaccatcgca tgccaatccg
     1021 ttgctttgcc acgtcttagc tgcatttcta accgccacca cgtatcgatt tcttttggca
     1081 cgattacttt ttttcgttcc tgctctagct ttttgttgta cagtcgcact tggcgctcac
     1141 tggcacgact gccccagtac gctgtttcta gcttgccaga acggccgtag attggcttaa
     1201 aagaaatcgg gtctacaata cgatactgag taataaagtc atccggcaca tctacaatat
     1261 cgcaggcaat atccgcccgg gaaaaatgcg gatctaaaaa taggtcatga atgaatcctt
     1321 tcatggaact agctaaaaac tggcctatct tattcggatt aaaatcaata cgaccttttc
     1381 cttcatgaaa tttaagtatt tcgatatacg caatatcttc tccatattga tcgaataatt
     1441 gccagccgtt tatcccaact gatttcaaat atccctgatt ttcgttcaaa cgcattaatt
     1501 gctcgaaatc gacaaaaaca actccatttt tcgtccaata agctatggta tcattcagtt
     1561 ttccgacaat tgtaattcga tcaatcgacc aatttaattt taggggggtg gtcgtacctt
     1621 gcacacttaa ccgcctgtta gaaatacggt taagtttttc gagctgattt atcgttgttc
     1681 ttttttctgc ccctcgacct tcaatttttg attgaatttc actcaactga ttttcatttg
     1741 ccattttcgt cctccaaacg aaaaaagaga cttttggaaa gaatttccag agttcacgta
     1801 accctaaaaa ttcatatcca aaaatctcta gcttgaagaa acctaaaaat attcgtataa
     1861 tgaacctgag ttacggagct aggtgaagta gtttccgtaa atagtcgaga gtgttagcgc
     1921 actgctcggc ttttattttg ctcttttttc tcgttttctt tgattatagc actcctcct
//
LOCUS       DAMEET010000170         1959 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.179, whole genome shotgun sequence.
ACCESSION   DAMEET010000170 DAMEET010000000
VERSION     DAMEET010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.179"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="PB780_002606"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="PB780_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDE0734608.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="PB780_002607"
     CDS             852..1040
                     /locus_tag="PB780_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDE0734609.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="PB780_002608"
     CDS             1053..1610
                     /locus_tag="PB780_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDE0734610.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="PB780_002609"
     CDS             1642..1884
                     /locus_tag="PB780_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDE0734611.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          715 a          305 c          404 g          535 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttctttt
//
LOCUS       DAMEET010000171         1747 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.182, whole genome shotgun sequence.
ACCESSION   DAMEET010000171 DAMEET010000000
VERSION     DAMEET010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.182"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1..591)
                     /locus_tag="PB780_002610"
                     /pseudo
     CDS             complement(1..591)
                     /locus_tag="PB780_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            669..1708
                     /locus_tag="PB780_002611"
                     /pseudo
     CDS             669..1708
                     /locus_tag="PB780_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341471.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          498 a          385 c          371 g          493 t
ORIGIN      
        1 gtgtgcagga gaaatttccc cagcatacga agaatacttt tttcgtctag aaaatttaac
       61 aaatagattt tcctcagtca tcaatctacg tacaactttt tcagaaagct ttttacccat
      121 attttttaaa gcttggtcaa ttctacggta accatacgca cagaagttat gattgaaaat
      181 cgtgataatt aattgacgtt ctttgtaata cttatctggt ttctcaagtg cttgtttttg
      241 ataaaaatag ctgcttttag gcaactggag ctgttgaaga agctctttca aggtgaactg
      301 atttcgtaag gcatcaatca atatcgtttt ttcttgattt gatagacaaa gaagattgac
      361 gcctttttct ttttttagca gttcagccgc tccttcgagt aatgcttttt cgagctgtaa
      421 ttggtgtact tgtttttgta agtcttctac ttcctttaaa agtagctctt tttgtttttc
      481 ctgatttttt aaagtcatac gcaatggaaa atcctttcca agaaccgtgt ttttccattg
      541 atatagagta actctactaa ttccttcaga ttcggctaaa tttttcctat tatataaatt
      601 gagttaaatt gtattcgttt ttcctcctat aggcgtatac taaagaaaac acttgaggag
      661 tggggaacat ggagacagaa cgccaaatcc gtgaattgga aaaagcgatg aatacgacag
      721 acaaccgccg gttgtacgag cgtttcctgg cagtcaagtt ggttcttgaa ggccacaccc
      781 ggaaagaagc cgggcacacc atcggccgcg acgagcatac tgtcagccac tatgtgaaaa
      841 actactgcca taatggaatc caaggtttag tctccaaaaa acaaagcgga cgaccttcac
      901 ggctgacaga tgaacaggaa gcggaactgg tccaaatcgt cgcctaccac actccgaagg
      961 aagtaggttt caaatcccgt gccaattgga cattggcgat tgtgtgtgac ctgattctcc
     1021 gggaatggag ttatggatat acgcaaaaag gcgtggcgga cctccttcac cggctaggac
     1081 taagctggac ccgtccgacg tataccctca aaaaagcgga ttccaagaaa caacaggtat
     1141 ttttggaaga aaccttcccg agcctaaaaa aaaattgctg aatagcgata ttgaccacct
     1201 cctattcgag gacgaaacca tgatccggga ttaccaagcc attggctgca cctggtttct
     1261 ccgcggccac cagcgaaaaa tccccactta tggacaacac aaaggggtaa aattgatagg
     1321 caccttggat tatgaaaccg gtcggatcat ttgtacagaa gaaggggcat acgacgcagc
     1381 ggccttcctc cgttttctgg agcggacgtt gcgggaatac cccaccggaa aaatcgtgat
     1441 ggtattggat aatgccctta tccaccacgc caagctgatc cagtcttttc ttgaagaaaa
     1501 cgctaggcgc ctggcactca tcttcctccc tccctacaga cctcagttga acctaatgga
     1561 aggcgtttgg aagtgactca aggaatcggt catcaacaat gttttttttg accacgtcca
     1621 gaaaatcaaa cagtcggtcc gcggtttctt agccgatgta aacgagcgtc cccttgaggt
     1681 gattgaccga ctgtgcgtgc ggatgtaaag tatttaattc aacctatata gttaattgta
     1741 gcctatc
//
LOCUS       DAMEET010000172         1730 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.183, whole genome shotgun sequence.
ACCESSION   DAMEET010000172 DAMEET010000000
VERSION     DAMEET010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.183"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(140..1288)
                     /locus_tag="PB780_002612"
     CDS             complement(140..1288)
                     /locus_tag="PB780_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDE0734612.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(1305..1709)
                     /gene="tnpA"
                     /locus_tag="PB780_002613"
     CDS             complement(1305..1709)
                     /gene="tnpA"
                     /locus_tag="PB780_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HDE0734613.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          510 a          300 c          273 g          647 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgccaccagt tgtcagaaga
     1381 caaaaacttt tagaccagaa catttctttc caaagaaatt gtttgactct tggaaagtca
     1441 cgttttatca atcttgagct ggcactttta taggcattga tgaattttgt caattctgtt
     1501 ttaggttgag ctttgaacat aatatgaacg tggtctttat catgattcca ctccactaaa
     1561 gtaatgtgat acgattctga aattctttca aaagtagttt tggcataatc agatatttca
     1621 tcatcaatca cttgtctacg atatttcgtt actaaaacaa ggtggtaata aagaagaaat
     1681 actgaatgat tatttgtatc taattccatg ttatatcaac tcatttcttg
//
LOCUS       DAMEET010000173         1718 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.184, whole genome shotgun sequence.
ACCESSION   DAMEET010000173 DAMEET010000000
VERSION     DAMEET010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.184"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..986
                     /locus_tag="PB780_002614"
                     /pseudo
     CDS             1..986
                     /locus_tag="PB780_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
BASE COUNT          590 a          320 c          279 g          529 t
ORIGIN      
        1 cggtcaagac cgagtacggg gaacttcatc tccagattcc gcgcgaccgc aacggcgagt
       61 tcaagcaaca gactgttcct gcttatagac ggacgaatga tatcttagag gagaccgtca
      121 ttcacctctt ccgaaaaggt attaccatgt cggaaatcgc agacttgatt gagaaaatgt
      181 atgggcatca ctacacgccc caaaccatgt ccaacatgac taaagtgctg actgaagaag
      241 taaatgcatt taaagttaga actctaaatg ataagtatgt cgctattttc atggatgcta
      301 cttatattcc tttaaaacgg caaaccgtct ccaaagaagc aatctatatt gccattggta
      361 tacgagaaga cggcactaaa gaagtactaa gttatgcgat tgctccgact gaatcgacct
      421 acgtttggaa tgagttgtta caggacattt actccagagg ggttcatgat gtcttactgt
      481 ttattaccga tggcttaaaa ggcatgaaag atacgattca ccagatttat cctaaagcta
      541 aataccaaca ttgctgtgtt cacgtttccc gtaatattgc tcataaagta cgtgtcaaag
      601 atcgaaaaga aatctgtgat gattttaagg ctgtttatca agctagctca aaggaagagg
      661 caaatacctt tttagggagt atgattgaga agtagcaaaa aacttatcct aaagtgacgc
      721 agtcactgat aaaaaatcaa gatttattga ctttttatga gtttccgcct ggtatccgcc
      781 gaagcattta ctcaactaat ttaatcgaat ctttcaagaa acaaatcaag aaatacagcc
      841 gcagaaaaga gcagtttcaa aatgaagaat cactagagcg tttcctagta tccatctttg
      901 atacatacaa tcaaaaattc ttaaatagaa gccataaagg cttccaacaa gtgacggata
      961 cattagcttc aatgtttact gagtaaccca ttattttgca gaaggacgag ttatttacac
     1021 aaaattattg acgctccctc caaattactt catcaaaaca ttgatataat ttttattata
     1081 tcagtattta ttcttgatac ataaatgaga aaataaatac tttaaatcta ttgactacaa
     1141 gtagtcatta agtcttcata agatgtacaa tatgacatta aaaaaataaa agcttacgac
     1201 catccgttaa atctgacact aaataatgga ctgctttctt ttgtattagc aggttacgtt
     1261 gactatgaat attcaccaga ttatttggag aaacctcttc aacacgagaa caatgatatt
     1321 aattttagat ttgtctacga gtcaaaaagt aaaacatggt tagttgatca attaagcacg
     1381 ataaacacaa gcttccatga attacctaaa cctgaaaatg aagttgagat ggtcattaaa
     1441 atcttctaaa aaagaagcgt tgaaggggaa atattccttt caacgctctt ttttatatgc
     1501 ttagaaacat aaataaattg tatactttct ttttagcccc tattactttt cacgaaaaat
     1561 atcttttttt cgacgctagc gtttctctac ttctaatact gtacacagtt tttgtattat
     1621 tgaaaaatta ctcattattt agcattaatc cacatcgttt tatcaatcga ccttcatact
     1681 aaactacttt aaagtcatct tccttctcgt ttttcatt
//
LOCUS       DAMEET010000174         1691 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.185, whole genome shotgun sequence.
ACCESSION   DAMEET010000174 DAMEET010000000
VERSION     DAMEET010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.185"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..182)
                     /locus_tag="PB780_002615"
     CDS             complement(<1..182)
                     /locus_tag="PB780_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDE0734614.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="PB780_002616"
     CDS             517..951
                     /locus_tag="PB780_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDE0734615.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="PB780_002617"
     CDS             974..1294
                     /locus_tag="PB780_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734616.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="PB780_002618"
     CDS             1435..1626
                     /locus_tag="PB780_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734617.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
BASE COUNT          639 a          255 c          358 g          439 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg a
//
LOCUS       DAMEET010000175         1690 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.186, whole genome shotgun sequence.
ACCESSION   DAMEET010000175 DAMEET010000000
VERSION     DAMEET010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.186"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            27..827
                     /gene="aph(2'')-I"
                     /locus_tag="PB780_002619"
                     /pseudo
     CDS             27..827
                     /gene="aph(2'')-I"
                     /locus_tag="PB780_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
     gene            811..1203
                     /locus_tag="PB780_002620"
     CDS             811..1203
                     /locus_tag="PB780_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDE0734618.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            1204..>1690
                     /gene="aac(6')-I"
                     /locus_tag="PB780_002621"
     CDS             1204..>1690
                     /gene="aac(6')-I"
                     /locus_tag="PB780_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(6')-Ie family aminoglycoside
                     N-acetyltransferase"
                     /protein_id="HDE0734619.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EH"
BASE COUNT          694 a          130 c          287 g          579 t
ORIGIN      
        1 atgataatgc cacaaatgtt aaggcaatga aatatttaat tgagcattac tttgataatt
       61 tcaaagtaga tagtattgaa ataatcggta gtggttatga tagtgtggca tatttagtta
      121 ataatgaata catttttaaa acaaaattta gtactaataa gaaaaaaggt tatgcaaaag
      181 aaaaagcaat atataatttt ttaaatacaa atttagaaac taatgtaaaa attcctaata
      241 ttgaatattc gtatattagt gatgaattat ctatactagg ttataaagaa attaaaggaa
      301 cttttttaac accagaaatt tattctacta tgtcagaaga agaacaaaat ttgttaaaac
      361 gagatattgc cagtttttta agacaaatgc acggtttaga ttatacagat attagtgaat
      421 gtactattga taataaacaa aatgtattag aagagtatat attgttgcgt gaaactattt
      481 ataatgattt aactgatata gaaaaagatt atatagaaag ttttatggaa agactaaatg
      541 caacaacagt ttttgagggt aaaaagtgtt tatgccataa tgattttagt tgtaatcatc
      601 tattgttaga tggcaataat agattaactg gaataattga ttttggagat tctggaatta
      661 tagatgaata ttgtgatttt atatacttac ttgaagatag tgaagaagaa ataggaacaa
      721 attttggaga agatatatta agaatgtatg gaaatataga tattgagaaa gcaaaagaat
      781 atcaagatat agttgaagaa tattatccta ttgaaactat tgtttattga tgataatatg
      841 gtttttgata ttgataattt aaaaggattt cttaatgata ccagttcatt tgggtttata
      901 gctaaagaaa ataataaaat tataggattt gcatattgct atacactttt aagacctgat
      961 ggaaaaacaa tgttttattt acactcaata ggaatgttac ctaactatca agacaaaggt
     1021 tatggttcaa aattattatc ttttattaag gaatattcta aagagattgg ttgttctgaa
     1081 atgtttttaa taactgataa aggtaatcct agagcttgcc atgtatatga aaaattaggt
     1141 ggtaaaaatg attataaaga tgaaatagta tatgtatatg attatgaaaa aggtgataaa
     1201 taaatgaata tagttgaaaa tgaaatatgt ataagaactt taatagatga tgattttcct
     1261 ttgatgttaa aatggttaac tgatgaaaga gtattagaat tttatgatgg tagagataaa
     1321 aaatatacat tagaatcatt aaaaaaacat tatacagagc cttgggaaga tgaagttttt
     1381 agagtaatta ttgaatataa caatgttcct attggatatg gacaaatata taaaatgtat
     1441 gatgagttat atactgatta tcattatcca aaaactgatg agatagtcta tggtatggat
     1501 caatttatag gagagccaaa ttattggagt aaaggaattg gtacaagata tattaaattg
     1561 atttttgaat ttttgaaaaa agaaagaaat gctaatgcag ttattttaga ccctcataaa
     1621 aataatccaa gagcaataag ggcataccaa aaatctggtt ttagaattat tgaagatttg
     1681 ccagaacatg
//
LOCUS       DAMEET010000176         1675 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.187, whole genome shotgun sequence.
ACCESSION   DAMEET010000176 DAMEET010000000
VERSION     DAMEET010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.187"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            45..443
                     /locus_tag="PB780_002622"
     CDS             45..443
                     /locus_tag="PB780_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDE0734620.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            427..1122
                     /locus_tag="PB780_002623"
     CDS             427..1122
                     /locus_tag="PB780_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDE0734621.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          639 a          240 c          331 g          465 t
ORIGIN      
        1 ataaaaacta ttagacaaat gatattttac gaggaggaac agagatgacg atctttgaaa
       61 cggttgtaac agatgttggt atagaagcag aagtattcag gaaagaaaaa ataatgattt
      121 tatttggaga tgaagcaccg gaaacattaa cagattattg ttataagatt gaattgaatc
      181 aggtaagaaa agaaataact tgtgatatga agctctgttt tgataaacaa atgtatgtaa
      241 tcacagcagt aggagatgtt gtccaaacta atttgaatga attaggacat atcacgataa
      301 aatttgatgg atctcatgag ccggaacttc caggaacaat gtatgtagaa gataaggaaa
      361 tgccgcattt aagaaagggg acaatcatta aaatcgatca agaggatcag aatgggggaa
      421 agctatatgg aaatcatgct tgatacagca aatacaaaaa gtatttattc ttgtcaacag
      481 tttttgaatt taacaggcgt aacaacaaat cctacaattc tgaaaaaaga aggagcgtca
      541 aacttcttct cgcatatgaa agaaatcaaa aaaataatcg gacagattcc tatccatatt
      601 cagacaatag gaagaacgga agaagaaatg atagaggatg ctcgagttat catagaagaa
      661 ctgggaaaag atacgtttat caaggtgccg gtgaatcaag caggccttca agcgatcaaa
      721 aagttgaaaa aagaaggtta tttgattacc ggtacagcta tttacacaga attacaaggg
      781 tatttagcta ttaataatgg agcagattat attgcacctt attataacag gatgcaaaat
      841 caaggcattg atgctatgaa agtgattgat tcgttgcgga ctgagatcga acgtacgcaa
      901 agaccaacaa aaatattagc agctagtttc aaaaattgtg aacaagtcac tacagccatt
      961 cgatctggag ctcatgcgat aacgttaggt aaagagctac tagatcaagt attagagatg
     1021 ccctcgattg ataaagcagt gaggggcttc caagaagatt gggtgcaaac tttccaagca
     1081 gaaacgattg gtgaactaaa aaaggtcgct gattacttct gaatcaattt ctccataaaa
     1141 taaacgatag ccttctcgaa aactcgagga ggctatctga cgattcaata ttcattaatt
     1201 taaagtttgg gtgactatta ctaactatta cttatttctc atttaaacga tgtattccct
     1261 aacctctaat acatccttgt aactctgaag ttacgccagt tttgaataaa tttcataaaa
     1321 acaaatgaaa agtgtcgcat aaaaatatct ttcgtgtttc tctagtataa cagaataaga
     1381 agaacaagtc cagaagggat atttttagtc ttttttaaaa taacttttca gtcgagtttg
     1441 agtttatgat agttgttttt gaagcgatac ggcaagtgaa aaaataggga agcatgaaag
     1501 aatctactta atggcgcatt gattcatgtg atagaaataa aaaacttttt taaattatat
     1561 cctcatgatt gtaacaaatc cagtgatttg ttttggtaca aataaaaaaa ggccccgatg
     1621 aagttgtggc aaaagtggtt tgcctcaaaa ataagaagga atttccaaaa atcac
//
LOCUS       DAMEET010000177         1644 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.188, whole genome shotgun sequence.
ACCESSION   DAMEET010000177 DAMEET010000000
VERSION     DAMEET010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.188"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..312
                     /locus_tag="PB780_002624"
     CDS             <1..312
                     /locus_tag="PB780_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HDE0734622.1"
                     /translation="QETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKES
                     SGSYTATNGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGW
                     Y"
     gene            complement(636..719)
                     /locus_tag="PB780_002625"
     tRNA            complement(636..719)
                     /locus_tag="PB780_002625"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(683..685),aa:Leu,seq:cag)
     gene            1247..>1644
                     /locus_tag="PB780_002626"
     CDS             1247..>1644
                     /locus_tag="PB780_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDE0734623.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSV"
BASE COUNT          508 a          308 c          276 g          552 t
ORIGIN      
        1 caagaaacgg ttcaaacgga aacacaagca gcgcctgttg ctgaaacaca acctgctcct
       61 gctgtaacag aaactgctgc tactccagca agtacaagct ctgcaaaaga atggatcgca
      121 caaaaagaat caagcggttc ttacacagca acgaacggac gttacatcgg ccgttaccaa
      181 ttagattctt cttacttaaa tggtgactac tctgctgcaa accaagaaag agtagcagaa
      241 caatatgtta cttcacgtta tggttcttgg gaagcggcga aagcattctg ggaagcaaac
      301 ggttggtact aagattcatt atttgaacgt ttaaacacat tcaaaatata gtaagaggtc
      361 atgcgattta ttctttcgca tgacctcttt ttgagtttaa gtttagtctt tattccttaa
      421 ctatcgtttg ccagtatttc ttgtatcaga atctataaat ctactatttg aatcaatcta
      481 ttaggtttta aactgtccca ccttcacaca aatttttttt gatttatttg aatctggttc
      541 cgttgtttaa aatttttcct atattctttt agataatatg ttattttctt tctcatcgct
      601 ccaggtgtac cttctcttta taaaaaaaca aattatgcgg ctaagaagac tcgaacttcc
      661 acgaggtcac cctcactagc gcctgaagct agcgcgtctg ccaattccgc catagccgca
      721 tcacatcttt tagtttagga ttacatgtgt tttttgtcaa gaacatattt ttgttctttt
      781 tcatttctgt acgccctccc ttatgccatc tacacaaata attcatccga gcaactccat
      841 catctattga gggttggtga aaaaatctgt tttatccata tgatcgattt caatgttaaa
      901 cgaacgactc tgggattagt aactatttta tttttttctt catttctttt ttcctctttt
      961 tctcgattca gctatcttct ttcatttgga tattgattat ttcttctgtt gattactatc
     1021 cttctggaat tctagacgct gaagtaattc acgttttctt tcttctagag atcataagta
     1081 acgaatgaac gaacttcaaa taattttttt attgtaattc atatttgtat tgttcgatga
     1141 ttcttaatac gatatgacac aataaataaa aataggcatg gctatttaca acaatagtgc
     1201 tatagtagaa aacaaatgaa taaggttact taaagaggtg aatacgatga aactgatcaa
     1261 actatgggaa gcagatttaa agaaagctta tcagctacaa actagctttg gaccaaatga
     1321 gagtggattt atcaacaatg cttatggcta tacatttgct gaatttttag attatgtaaa
     1381 aaaatgcgaa aaaacgtctt taggaatcga tcttcctgag agttacgttc ccgcaaccgt
     1441 ctttatttta gaaagtgatg aacatgagta tgtaggaatt tttaatttgc gccataaact
     1501 gaacgaaaca ttaagaaacg gcgctggcca tattggttac ggcatctccc ctaattatcg
     1561 taaaaaagga tatgccacac aaggattagc attagtacta caagaggcaa aaaaaatcgg
     1621 cattgatgag gcacttttgt ctgt
//
LOCUS       DAMEET010000178         1605 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.189, whole genome shotgun sequence.
ACCESSION   DAMEET010000178 DAMEET010000000
VERSION     DAMEET010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.189"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            383..>1605
                     /locus_tag="PB780_002627"
     CDS             383..>1605
                     /locus_tag="PB780_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDE0734624.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFNDSYHFDQIVIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFS
                     RMFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQH
                     FLENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGK
                     GRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQ
                     VIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQ
                     SQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARK
                     FTRLVDTLLRKHQLYT"
BASE COUNT          524 a          308 c          286 g          487 t
ORIGIN      
        1 ttgtctaata gtttttattc aaaatgtttc tagattataa aatttattga ataacacatt
       61 cctaacggaa tttgtttcga tattgtgtga tttctcagag tacaatggat gtatggaaag
      121 gatccaacgg gaatttgcta tttcctcctg tagcgtttgt ctaccctgtt tagtctgtta
      181 cccgaaccac tgttatcact accaactcat tagctgtttc gataatggac tagactttca
      241 aatagtagtt tggaaggcag cttctagctc ttttatcacc gtattctatt aggcgaggtt
      301 gtggctatac agtgagtttc tgggaaatcc cacagtatcg aagcattttc caaaatttat
      361 atcgaaagga ggtccttctt ccatgaaatt atttgtcggt attgacgtta gctctgaaaa
      421 actagatgtt tgttttttaa cagacgacaa tcaattgtct atcttatctg aaatctctgt
      481 tgccaatgac atcgaaggtg cttcattcat acgagaaaca attcttgaat tcaatgacag
      541 ctaccacttt gatcaaattg tgattggcat ggagtcaacg tccatgtaca gcttccatcc
      601 ttccatgttt tttcatgaag atgagaaact aaagaagctc aacacacttg taacgattga
      661 aaatcctttt cgggtgaaac aatttagtcg catgtttgat gagaataaaa ctgatcgaaa
      721 cgatgcatta agaatcgctg atttcttgcg tattcaacga ttcacaactt cccctatcaa
      781 agaagagaaa tacatggcct tacagcgttt aacacgtact cgctatcaac ttattaagca
      841 attgattcgt acaaagcaac attttcttga aaatctaacg tacaaatgta atacgcttgc
      901 tcgagaaatg cgagacgaat cgacgagtct ttttagtgca accctcattt cactcatgac
      961 ggaagatttc acattggatg aacttgctga gcttccttta gaagctttct gtgatttact
     1021 tcaagaaaaa ggaaaaggcc gtttcaagca accagaaaaa atcgctaaag caattcaacg
     1081 tgcaattacc atgagttacc gcctaggtgc tcttgcacaa gaatcaatta atgttgtttt
     1141 aagtgttctc gtacgagaaa tacgagcact tgaaaaaaat atcaaagaat tagataaagc
     1201 catcgaacaa gtaattgttg tcattccaga atatcaatgc ttaaccagta ttccaggcgt
     1261 tggtaaagtc tatgctgccg gtttaatcgc tgaaattggt caaatcgaaa gatttgaaga
     1321 tcagacaaaa ttggcaaaat acgctggatt aagttggaaa gtgaaccaat ctggaaacta
     1381 tcagtcgcaa aatacacctc tgacaaaaca aggaaatcga tattttagat actacttagt
     1441 tgaagctgcc aactctgtaa aaaattatct tcctgaatac aaagcttttt atcaaagtaa
     1501 atataaggag gttccaaagc atcaacacaa acgtgcactc gtcctaaccg caagaaaatt
     1561 tacgcgtctg gtggatacgc tactacgtaa acaccaactc tatac
//
LOCUS       DAMEET010000179         1578 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.190, whole genome shotgun sequence.
ACCESSION   DAMEET010000179 DAMEET010000000
VERSION     DAMEET010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.190"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(221..1240)
                     /locus_tag="PB780_002628"
     CDS             complement(221..1240)
                     /locus_tag="PB780_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HDE0734625.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1251..1457)
                     /locus_tag="PB780_002629"
     CDS             complement(1251..1457)
                     /locus_tag="PB780_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDE0734626.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          439 a          331 c          273 g          535 t
ORIGIN      
        1 ccgtctgctc tgaccaactg agctaagggt actggttgtt gccacataaa gccataaaca
       61 atcaaccagt agaatgtgtg gcaacaaacc tgttatcgca tagcttggag tgtgactatt
      121 atgggtgata gtgaagatat gcgataacat cactatttta tcgaatgatt tttatagttg
      181 tcaatatagt tatgtaatgc tcctcaacga ggagctattt ttatcgttta ggaatattta
      241 aataccaacg tttgtcatgg aaatcttgcg caccgccttt agtgtttccc tctggatcat
      301 tcgttgcccg catcatgaca tagactttct tattagggaa gttacgcatg ttaaaagata
      361 catgatagcc aacatttcca gaagtattat aagcttgatt tacatctgat ctataaattc
      421 catcagctct tactcgagct aattctttcc cagtattgta atccataatg aagatatact
      481 cgtatttata gttagcaatg tgccatccag ctacatgcaa atttgcattt tcgatttctc
      541 caaactgatc aatgtgggcg taattcgttc catctgtcag tgtaggatta gctgcaccag
      601 ctcgtgttgg atcaatgaca ggcttgtttt cagaagttgt tggattttca tcggtaaatc
      661 catgagctaa atcatatgct aatttttctt tacttacgcc catttcagaa agataaccgt
      721 aaggatctgt atgatcgccc cagatgtttt gtgttaccca taaatgcgat ttgattcccg
      781 gttggttata aggagtgtcc aacgttaatg gaataccata tttcattgct gaatctctag
      841 ccaattcaac gtatgccttg tagtttttct caaacgttgc tttatcatgt gtgtgttgta
      901 actcaatctg cacaggactg ttggcattag catacgaacc agcaccgtac tgtacataac
      961 caggttgacc gacttgataa acaattccgc cgtctcccac aatataagca gtataagcgc
     1021 tagtccatga acgttgcata tactgcgctt cattgcgtcc tgttgctgtt tcattagccg
     1081 tttcatgcag taaaatatac tgattatttg ctacttgtga gctaccttca tttgcgccca
     1141 aattaaattc attgttgata gtataggcaa atccgttaat tggcaataaa aaaagagccg
     1201 ttaacaggct catcgcagta atagtaattt tctttttcat atctatcctc ctatttatct
     1261 gatgaaattt tcttatacgt tcgatttgat acacctaaaa tactccctag aaatgctcca
     1321 aaacctgtaa taatcatcac cgttatatct gtgtaccgcc agttaacacc ttttccgaca
     1381 agaccgacaa aagtcgctaa agcggggata actactatag cgatccattt caaaatatca
     1441 aaggttctat tgttcatact atccacctcc cttcattggt tgggctactt tctttttcaa
     1501 tcgcttgttt tcttctatca atatttctct ttctgaaagc aaggcatcaa tttgcccgtc
     1561 cagttgttca attttatc
//
LOCUS       DAMEET010000180         1560 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.191, whole genome shotgun sequence.
ACCESSION   DAMEET010000180 DAMEET010000000
VERSION     DAMEET010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.191"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            132..344
                     /locus_tag="PB780_002630"
     CDS             132..344
                     /locus_tag="PB780_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734627.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..1024
                     /locus_tag="PB780_002631"
     CDS             506..1024
                     /locus_tag="PB780_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HDE0734628.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1031..1543
                     /locus_tag="PB780_002632"
     CDS             1031..1543
                     /locus_tag="PB780_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734629.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
BASE COUNT          615 a          181 c          242 g          522 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa aagatataaa ttcaattgat aattattata tacaatttaa
      781 aggttttgac tttaatagct ctaccgaatt taagttagca ctttatgcac aattttttat
      841 tgaaaaatta aataggtttc aagaaatagt tgaagatagt gattttaaca cttttaatag
      901 ccataaaccg atgagaggta cttatataaa atttctcgaa aattataatg atttaaaatc
      961 taccaataat tatcaatttg gaaacttgtc ttatgagatg ataagtaaag ttctaagttt
     1021 gtgatgataa atgaaattaa atagtataaa tgatttattt gctattatag gtgttattgc
     1081 taacatactt ttttcgtgtt tgaattttag atttactcaa aagaacaaaa agaacaaact
     1141 cagtatagaa caaatgaaaa aagacattga aactgattta aaaaatgacc ataaagaaag
     1201 aagaaaaatt gaagaatata cttctttaat ggaaaagtgt aaagcaactg atgcaatttt
     1261 tagcttttta agaaaaagtg atgagataat cacacgtgaa aatatagatt tagaaaatga
     1321 gattagtaaa cttgaatcta tcttttccga tataaattct cttaagtatt taatcgtcga
     1381 agaaaattcg gatagctttt tagaatttga aaaatatcta acagatctta tagataatag
     1441 aaataatgaa ttaatgagac aaaacattgc aagattcctt actaaattag tttctgaatg
     1501 gaaaagtatt atttctcaaa atatggataa attaagatat taaggatgtt agttttaaaa
//
LOCUS       DAMEET010000181         1555 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.192, whole genome shotgun sequence.
ACCESSION   DAMEET010000181 DAMEET010000000
VERSION     DAMEET010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.192"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            41..1507
                     /locus_tag="PB780_002633"
     CDS             41..1507
                     /locus_tag="PB780_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDE0734630.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          430 a          290 c          327 g          508 t
ORIGIN      
        1 ataatatttt ctgaaaattt aaagaattgg ggtatagatt atggaaaaac agcaagacaa
       61 gggcttcttc gggcagccga agggattgtc aactttgttc ttcactgaga tgtgggaacg
      121 atttagttat tacggtatgc gtgcattact tatctattat atgtacgata cagttgccaa
      181 cgggggactt ggattaccac gagcaacagc attagctatc atgtcgatct acggatcttt
      241 gatctacatg tctagtatcg tcggcggatg ggtatctgac cgagtgttag gagcaaaacg
      301 cactgtattc ttcggtggag ttttcattat gcttggccac atcgtcttgg ctttaccatt
      361 tggtgtaagt acattgtttg tttcgatggc attgattatt atcggaaccg cctttttgaa
      421 accaaatgtt tctggcatgg tcggacatct ttattcaaag actgacttgc gaagagatgc
      481 aggattttcg attttttaca tgggtgtcaa cttaggggca ttattagcac cactaatcgt
      541 tggtacaatc ggtcaagaat ataactatca tttagggttc tctttagctg ctattgggat
      601 gttctttgga ttattacaat acatcatcca aggacgcaag acattagacg gagtgggtac
      661 aacaccgccg aatcctttga caactgaaga acgtaaagga ttcatccgta acttgatcat
      721 cgcacttgtt gttatcgctg cagtattcgg cggggcacaa ctaactggtc atctgacaat
      781 tgactttttt gtcaatacaa tcagtgtatt ggggattatc ttaccgttgt attactttat
      841 caaaatgctg acttcaaaag atgtaactgc tgaagaaaaa ccaagagtat gggcttatat
      901 cccattattc cttgcagcaa tcattttctg gtcattacaa gaacaaggtt catctatctt
      961 agctttattt gctagtgaac gtacacaatc tacgctattt ggtgtaacga tccaagcaag
     1021 ctggtatcaa tcattgaatc caattttcat ctttattctg acaccagtat tcgtaacgat
     1081 gtggacgaaa cttggtgaac gtcaaccatc tacagtagtg aaattctctc ttggactttt
     1141 atttgcagga ttatcgtact tgctattgat gttgccgggt atgctttatg gtacaagtgg
     1201 aaaagtaagt ccattgtggt tgttgggaag tttcttcatc gtggttattg ctgaattgtg
     1261 tctttcacca gttggtttgt ctgtgacaac aaaattagca ccacgagcat ttgaatcaca
     1321 aacaatcgct atttggttct tagctgatgc ttcttcacaa gcaatcaatg cacaaattgc
     1381 acgcttctac acaccgggaa cagaatctgc ttacttcggt attatcggtg cagtagcaat
     1441 cgtcggtggt atcttgctat ttactgtgaa agaaccaatc aaaaaactaa tgggctctat
     1501 tcattaatca atgaacataa aaacaggaat tgcttatttg cagcagttcc tgttt
//
LOCUS       DAMEET010000182         1515 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.193, whole genome shotgun sequence.
ACCESSION   DAMEET010000182 DAMEET010000000
VERSION     DAMEET010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.193"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            287..694
                     /locus_tag="PB780_002634"
     CDS             287..694
                     /locus_tag="PB780_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734631.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="PB780_002635"
     CDS             708..1109
                     /locus_tag="PB780_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734632.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="PB780_002636"
     CDS             1111..1482
                     /locus_tag="PB780_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734633.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          575 a          185 c          264 g          491 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt ttttt
//
LOCUS       DAMEET010000183         1482 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.194, whole genome shotgun sequence.
ACCESSION   DAMEET010000183 DAMEET010000000
VERSION     DAMEET010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.194"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..484
                     /locus_tag="PB780_002637"
     CDS             <1..484
                     /locus_tag="PB780_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDE0734634.1"
                     /translation="KEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKEQ
                     QVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFSN
                     NQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVIK
                     "
     gene            complement(691..1092)
                     /locus_tag="PB780_002638"
     CDS             complement(691..1092)
                     /locus_tag="PB780_002638"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDE0734635.1"
                     /translation="MSDKFSKKISSDIKEDFKAIFKTASIELAGERKQLIMDKYGEDK
                     KVATACKILDEGFEDAMQVMVLPENIRQRLRTTNVLERLNEEVRRRERVVRIFPNCDS
                     VIRIIGALLMEKDDEWTSAPRKYLEFDRNEI"
     gene            complement(1123..1221)
                     /locus_tag="PB780_002639"
     CDS             complement(1123..1221)
                     /locus_tag="PB780_002639"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDE0734636.1"
                     /translation="MKERGLSGVKMVISDAHIGLTKAVKEYLLCAS"
     gene            1319..>1482
                     /locus_tag="PB780_002640"
     CDS             1319..>1482
                     /locus_tag="PB780_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDE0734637.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTG"
BASE COUNT          482 a          277 c          287 g          436 t
ORIGIN      
        1 aaaggaagcg atcgatcaaa tcaagacggc tgtcttgtat cctcataacg gattaccgat
       61 tttattgatg ggtgtaagcg gttcaggaaa aacctttttg gcagaaaaaa tttatgaata
      121 cgccaaggaa caacaggtga tagaatcaaa tgctccattt atcgcctata attgtgctca
      181 atactttaat ttctcgatta acaagatcgt agaaaattta acgatccttg atgcaatgaa
      241 ggttttgaga ttggtacaaa aggccgtcaa agaactcgaa aaactaatgg ggatcgaatt
      301 ttcaaacaat caattgttct tgctttacat gcactgctgc tcaatgatcg aacgtatttt
      361 acgcaaagaa agtgtagatg aacaagcaga tatcaaagaa tatattcaaa aagagggaca
      421 taattatgaa atatcaaaat acccggctta caaaacgaag tttgaaagta ctgtaataaa
      481 gtaagattta aataagcata aacaaggtaa gaaccatagg ggctgtgtca agtccaaaat
      541 cttgtgtaaa aaggaaatct caaagtacga tgttttgcct tgttgagcct agctacggtc
      601 caatatgctg gtaagaagtg tcaagagcaa aaagcgaagc gtctcttgat acttctcacc
      661 agtatattat actattggcg gcttaatgtg ctaaatctcg ttacgatcaa attcaagata
      721 ttttctaggt gcagaagtcc attcgtcatc cttttccatc aataaagcac cgatgatacg
      781 aataacagaa tcacaatttg ggaaaattcg gacaacacgt tcacgacggc gtacttcttc
      841 atttagtctt tcaagaacat tcgttgttct caatcgttga cggatatttt ctggtaaaac
      901 cattacttgc atggcatctt cgaagccctc atccaatatt ttacaggcag ttgcgacttt
      961 cttgtcttcc ccatatttat ccataataag ttgtttacgt tctccagcca gctcgatgct
     1021 tgccgttttg aagatggctt taaagtcttc tttgatatcg cttgagattt tcttggagaa
     1081 tttgtcggaa atgttgcgaa ggagatgtgc ttggcatctc tgctagctcg cacaaagaag
     1141 gtattctttc acagcttttg tgagccctat atgcgcatcc gagatgacca tctttactcc
     1201 tgaaagaccg cgttctttca atccgagagt gtaaaatatt ttgtgtaaat agaaaaaagg
     1261 aagtcccttc agtagaatag agttaccaca acacattcac agaaaagagg acttccatat
     1321 gaacgatttt actacagaaa ttctaaagac tctagcgaat aaaggcgatt tgaatgaatt
     1381 attccgtgtc catttggaaa aagctgtcaa tacgcttctc aaaacggagt taacggcttt
     1441 cctcgattac gaaaagtacg atcgcattgg ttttaacacg gg
//
LOCUS       DAMEET010000184         1454 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.195, whole genome shotgun sequence.
ACCESSION   DAMEET010000184 DAMEET010000000
VERSION     DAMEET010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.195"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            108..1040
                     /locus_tag="PB780_002641"
     CDS             108..1040
                     /locus_tag="PB780_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HDE0734638.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1112..>1454
                     /locus_tag="PB780_002642"
     CDS             1112..>1454
                     /locus_tag="PB780_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734639.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQ"
BASE COUNT          639 a          169 c          227 g          419 t
ORIGIN      
        1 atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt
       61 caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa
      121 aagaaaagga atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag
      181 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
      241 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      301 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      361 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      421 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      481 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      541 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      601 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      661 gagaattaaa tactagagcc ggttatgtgt atgtaattag taatattggg tcttttggtg
      721 aagacatata taaaattggt atgactagaa gattagaacc tatggataga ataaaagaat
      781 tgagtagtgc atcggttcca tttgaatttg acgttcatgc gatgattttt tcagaggacg
      841 ctccgtcttt ggaaactaaa ttgcataatc attttagaaa acaagaagtt aataaaatta
      901 atcaaagaaa agaatttttc aaagtttcac tagacgaaat agagaaagtt gttttagaaa
      961 attataatgg aacagtaaca tttacaaaat tagctaaagc tgaacaatat agacgatctt
     1021 tagaattgtc aaaagattaa atatataaaa agccttcggg ctttttcttt taacaattaa
     1081 taggaacgta tattcgaaag gaatagaaat aatgtggtta gaaaaaataa atgacaaaaa
     1141 atttaagtac accgaacgtt atacggatcc cttaactgaa aaaaagagaa aagtgtccgt
     1201 tactttatct agcaattcac gccaagcttg gaatcaagct aacttacttt tgaatgaaaa
     1261 aatcgcagaa aaaataaaac gaaatgaaga attaccacta acttttggag aattaaaaac
     1321 taaatgggat gaaaaataca agccaactgt aaaagaaagt tcctatcgaa caactcaggt
     1381 ttatctatca ttaatttcaa aatatataaa agatgatgta cttgtaaaaa acgttaatag
     1441 taatcttata caag
//
LOCUS       DAMEET010000185         1420 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.196, whole genome shotgun sequence.
ACCESSION   DAMEET010000185 DAMEET010000000
VERSION     DAMEET010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.196"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(930..1343)
                     /locus_tag="PB780_002643"
     CDS             complement(930..1343)
                     /locus_tag="PB780_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HDE0734640.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          524 a          236 c          209 g          451 t
ORIGIN      
        1 ataacataat aagaaagtaa agtggattga tttgagatct ttttatactt caaaaaagat
       61 aacacccaac tttcaaaaat atgttttgga agttgggaca aacgagttaa tacattttaa
      121 tacattattt tttattcaaa aaaaatagca caatggcaaa gatcgtaaga gagatcataa
      181 gccccaatga atgactaaaa agaaaagcag aatcacatgc catatcatcc catatttctt
      241 caagaaaata aataagcggg aaaaagggaa ggaatagtag aacatcgatc cagcaccatt
      301 tttttcttct tttgaaataa cataaaacaa taaacatgaa cgtgcaggga agtaaaaaca
      361 aagctttact cataactaac aaaataccag ctccttttat ttttttctcc cccacattca
      421 aagagtaaca atggtatgta cttaataata gaaaaatgga tcaacttgta tgaataacag
      481 ctgaaaagat ctgcaccaaa taaaaaactt ccagccaaaa ctgactggaa gtttattttt
      541 ttctgaaata ttctaacaca aatatgaaaa agatggccga agctaataag ccgatttcgt
      601 agcaaaacaa tgagatacca gagtcgtaat aataaaatcc tctaatctta tggataaaat
      661 agatcatcgg acaagcagat aagagtaagt atttgttagg ccttttgaat aagtaaaaga
      721 aagcactggt cattatgata aatggtatcc aaaacaaaag tttacttaag acaatcaaat
      781 gatcaactcc catatttttt accaagagag atcataacat ctttatcgta aaaacaaaat
      841 atttttttgc tcattttgaa aatttaaaca tactcgttct tatccaaata tataaaaatc
      901 gcccctcatt catcgagagg cgataagttc taatttaact caattttttt tgtttctttt
      961 ggctgataag ctgttttagg taataccaat ttcagcacac cgtttttaaa ggttccagtg
     1021 attttttctt catcaacatt aggaagatag aatgaacgtt tgtaagagac cgaatgtctt
     1081 tctttccgta catagttgcc atcttcttta tcttctgtat gggactcatg attggcggag
     1141 atcgtcaaag tattatctga atattcaact gtcgtgtctt ctttgtcaca gccaggcata
     1201 tcagcagtta gtttgtattc tttttcattt tcgactaaat ctacttttgg ataacttgaa
     1261 acacctaaaa aatcattgaa tgctggacta aacacgtcat tgaaatcagg gaacatgtcg
     1321 cgaatgctag gtacatttgc catttttgat catcctttca ataagtattt tttatttatc
     1381 cctatttacg taataactct attttgaaaa aaatgcaagt
//
LOCUS       DAMEET010000186         1418 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.197, whole genome shotgun sequence.
ACCESSION   DAMEET010000186 DAMEET010000000
VERSION     DAMEET010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.197"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(150..1337)
                     /locus_tag="PB780_002644"
     CDS             complement(150..1337)
                     /locus_tag="PB780_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HDE0734641.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          413 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttca
//
LOCUS       DAMEET010000187         1418 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.198, whole genome shotgun sequence.
ACCESSION   DAMEET010000187 DAMEET010000000
VERSION     DAMEET010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.198"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..170
                     /locus_tag="PB780_002645"
                     /pseudo
     CDS             1..170
                     /locus_tag="PB780_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(215..1341)
                     /locus_tag="PB780_002646"
     CDS             complement(join(215..1113,1113..1341))
                     /locus_tag="PB780_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDE0734642.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
BASE COUNT          424 a          233 c          230 g          531 t
ORIGIN      
        1 ttaaattgat ttttgaattt ttgaaaaaag aaagaaatgc taatgcagtt attttagacc
       61 ctcataaaaa taatccaaga gcaataaggg cataccaaaa atctggtttt agaattattg
      121 aagatttgcc agaacatgaa ttacacgagg gcaaaaaaga agattgttat tttggatttg
      181 agtcaatatc ttggacacat tttagtagag acggttaagc cattttttct tttgctaatt
      241 cttcaaattc ctgtggggtt agatagtgaa tcgagctatg gattcgattt cggttataaa
      301 atccttcaat gtagctaaat agcgctcgat ttgcttcttc aaaatctgag tagctagtcg
      361 tgtagacttc ttcttttttc aatgaggcat ggaaagattc gattccagca ttatcataag
      421 gtgttccttt tctactataa gaatgcctta ttttgttggt tttaagccat tgttccactt
      481 ctcttgctgt gtattggctg cccaagtcag tgtgtagaat cattccttct ggaatgtgat
      541 aatgtatttt tgctttattc aatgtttgaa tgacacaatc cacagtcatt cgttttgaaa
      601 atgtataact aataattttt ttcgtgtgta aatccatgat tgaagataag taacaccaac
      661 catttttctt agtaggaatg tacgtaatgt cagctgccca tttctcacag atcgtttcag
      721 tagaaaagtc ctgatttaag agattctctt ttgaaatgat cggtttatta gacctttgag
      781 gtctatattt tttaactaca atcgacctta aatttaattg tttcattagc ttttggacat
      841 gtttaagaga tactgaaatc ccttctttta ataaaacttg atggatcttt gtggcaccat
      901 agcgttgttt attcgtaaaa aaaattgttg aaatcttctt tttcaactta ttatttctga
      961 tttctgtagc tgtcaacggt ttattcttat aaaaataata gacacttcta gggatttcta
     1021 ataaccgaca taacaaagaa acagtgtaat ccttacacca tttttcaata aattgaacaa
     1081 gcgaatttag gttttccgag agaatatggc tgcggctttt tttaagattt caagttcctc
     1141 atttaaacga gcattttctt ttctcagttc agctacttct tttccagtca gtcctgtgga
     1201 ctgatcaggt aaatataaat tcttccattt gtaaatcgtt gcttcagata agccatattc
     1261 tttcgctaac cctcgaacag aacgtccagt ttgattcagt tcaacaatca tttgtttaaa
     1321 attttcttca tatcgggtca taaaaattca tcctctcgta gataagttta atttattctt
     1381 gtctctacga aaagctgtct aagatttgat actaacac
//
LOCUS       DAMEET010000188         1390 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.199, whole genome shotgun sequence.
ACCESSION   DAMEET010000188 DAMEET010000000
VERSION     DAMEET010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.199"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            5..415
                     /gene="spx"
                     /locus_tag="PB780_002647"
     CDS             5..415
                     /gene="spx"
                     /locus_tag="PB780_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDE0734643.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            429..785
                     /locus_tag="PB780_002648"
     CDS             429..785
                     /locus_tag="PB780_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HDE0734644.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          509 a          209 c          259 g          413 t
ORIGIN      
        1 gaaaatgata aaagtatata cttcgcctag ctgtacttcc tgtagaaaag caagagcatg
       61 gttgcaagcg aatcaacttg aatttgaaga gaagaatatt tttgccgaac cgttaacaga
      121 aagtgagatc aaaaagatct tggcagcgac tgaaggagga gttcaagata ttatttccac
      181 tcggtcgaaa atttacgaaa agctgggcat agattttaac gaattaactt taaagcaaat
      241 gatcgtctta ttcaaagaat atccttcatt attgagacga cctattttga ttgatgaaaa
      301 aagaatacta atcggattta atgaagatga gatccggtca tttgtccctc gtgaaattcg
      361 atgtgttgcc agaaatctga tctatttaca aaacaataac atcttaaatt catagggagg
      421 gagccttcgt ggaagtgaaa gtctatggat cagcgcactg tgtaacaagt ctgaaggcga
      481 aacaatggtt agaacaccat caattgcctt atcaatttgt tgacttagaa gaacgcgatc
      541 taagtacaga agaagcagaa gaattatgca gttttgaaga agtgcaagca gaacagctat
      601 ttgcgacttg gtctgaggct tttcacaaca ttgcgattga cctttcctgt gtaggaaaaa
      661 aacaactgat ttttttatgc acacaacata ccaatctcct cagacgtcct ttgattatta
      721 tcgatgaagt tctatttatt ggatacaatg agcagttgat ggaacagctt atcttaaata
      781 aatagacttt aactgtttta atcaagtcac tgagcatttc atacagttgt aacagtcgaa
      841 cacttagcat caagcgtgtt atgtgttctt tttttgactt tatttttcta ctatagagac
      901 atgctcaatt tatgtggtca aaattcaaat ctaggtgggg atatagagtt aaaaatatat
      961 tgacatgtgt aaatttacat gatatattca tttctgttgt tttaagtagc aaagcaataa
     1021 aatagtattt taaaaaacat ataaaatagt tgttgacatg ttagaaatga catgataaga
     1081 taactgagtt gttaaaaaag aaccacttgt gaagcttgat tctatcgaaa aaaacttcaa
     1141 aaaagttctt gacaaataac aaatgataag ttatgataaa tgagttgctg taagatttcg
     1201 aaaaacgaaa tcaaaaaaac ttttaaaaaa gagttgacaa ttacttgtct ctctgatatg
     1261 atataaaagt tgctacggca gccaaaaacg aatcggggaa cactcctgat gaagtagacc
     1321 tttgaaaact gaacaaagta agacaaacca aatgtgtagg gcgtcttgat tcaattcaag
     1381 acaacaaaca
//
LOCUS       DAMEET010000189         1387 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.200, whole genome shotgun sequence.
ACCESSION   DAMEET010000189 DAMEET010000000
VERSION     DAMEET010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.200"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            341..883
                     /locus_tag="PB780_002649"
     CDS             341..883
                     /locus_tag="PB780_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734645.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            1039..>1387
                     /locus_tag="PB780_002650"
     CDS             1039..>1387
                     /locus_tag="PB780_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HDE0734646.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          551 a          167 c          260 g          409 t
ORIGIN      
        1 tgatagtaga tttaaaactt tgcaacagaa cccttttgaa taggttcttc gttgacgtct
       61 tgacatgaaa aatcctccag tgtgtttttt attattctac acaccttatt ttttctgtct
      121 agtctagtgt agccgattca aattgatgaa gcagatccca aagggctgct tcatcaattt
      181 tttcgtaagt gtccgtacac ttactgtgct tgctcatata ttaatcgatc atatactaat
      241 cgtgtaagaa caactttaag cataatactt atattgttat accatagttt ggaaaaataa
      301 aaaattatat ttcatcaacg tttcataaaa ggtgtgtaga atgttattga aagtagctat
      361 aaaatgctat tgtgtaaaaa ataaaggagg aataatttat gaaaaaatag taccaagagg
      421 agcatgccta ttacttgcat tatgtttgag cttaagcgca gttgggtgtc aaaaaagtaa
      481 tgaaaacaat aaaataaaag aaggacaatc cataagttct gaagaggaag gcatgtcaat
      541 taatgcaaat ggcaaaaatg aaacttttaa gccatcagat tatacattgg aggcaaagaa
      601 agaatatgtt tatgaatatt tagggcttaa atttaaactc tcagacaaat ttagaaatta
      661 tatagctgat aaaaagatag cgatgttaga tgatcaaagt cctatagata aagaactaaa
      721 gtatgctatt ttgacatttg agaaaatgac agaagaacaa aagaatgcag ttatagaaaa
      781 gatgggcgat ggatataaaa attggcaaaa tgagcttgaa agaattggga ctattggtat
      841 atttgaaaaa aatacatcag aagaaaaaaa tctaaaatta taaaatgtga tactcatact
      901 aaaataggag tttcaagtga tggaaaatac gattgctatt taagcacaaa tagcggagct
      961 gaaagtaatc ttctggatga attaaaaaga actgagattc aaattataga taaaaaagaa
     1021 cgtcctaaaa atggttttgt gctatcagaa aaaactgatt tagaaaatac agaagcattt
     1081 aacaaagaat cggttaaaga tttacgtaaa ctaccaacaa aggacataaa tggcaaggat
     1141 tttacaagta aagattttga gaagtatgat cttactatgg taaatgtatt tgcaacttgg
     1201 tgtacagctt gtgtaaagga aattcctgat ttggttgagg ttcagaatga aatgaaaagc
     1261 aagggtgtaa atatcgttgg agttgttaca gatcctgttg atgataacgg tgaaaacaag
     1321 gaagcaattg aaaagtcaaa gttaatacat gaaaagacaa aggcttcata tcctttctta
     1381 atgccag
//
LOCUS       DAMEET010000190         1377 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.201, whole genome shotgun sequence.
ACCESSION   DAMEET010000190 DAMEET010000000
VERSION     DAMEET010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.201"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(46..1365)
                     /locus_tag="PB780_002651"
     CDS             complement(46..1365)
                     /locus_tag="PB780_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDE0734647.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
BASE COUNT          471 a          185 c          102 g          619 t
ORIGIN      
        1 aataagtctc tatttttcaa taactattgc aaaattatat cttaattaag aaaatatttt
       61 tattttaaag ataaaaaatt cttcatcctg caatacttta tattctatat tgtaattatt
      121 cagaatatta cttacaatat ataaacctaa tccattacta ttttccttat ttaaatcaaa
      181 tttaacatca aatattttat ccatattcga aattttattg ttaccgtatg aattctctat
      241 atataaccaa tcattaacta tcccaatatt aattacccca tttacatcag tatattttac
      301 cgcattacta atcaaattag atagaataat ctttaaagct gtttttccta tataaacatt
      361 ctcgtctaat atataattat tgacagttat tttcttttga tttgctaata tttcatactt
      421 ttttaatatg tcttctaatg tatcatttat tttcaaatat tcttcatcat tattcaaatt
      481 ttctatagaa tgaactgata atatttgaga aatatttttt gttaaactat caacgatttc
      541 aatacattca ttaatataaa tatctctttc tttatatttt ccaatattgt atttcatgtt
      601 ttccagtatt attttaagac tagcaagagg ggttttaagt tcatgggatg cacctttaaa
      661 aaagtcatat tttaactttt ctaattttaa aatttcttta tttttaaatt ccaaatcgtc
      721 aattgttctt aataaagtag aatacaaatc attaatttgt tgttttaatt ctccaacttc
      781 atcattagag ctaactttta aactcatttt tttatcaagt ttcatcattt tatcagtaac
      841 aatttttatt tcttgtatat tattttttat tgattttgca taaattaaag aaataatagc
      901 agaaaataaa attgatatta ataaagaata tggtaaaaat ttaaggctta aatctttcgc
      961 atctttttgc atatcagctg tagaaacaaa ttgtagattt gtttttttac catcattaag
     1021 ctttatttct ctttcttcaa ttatcaaaga attactatca ctttctaaat taacatttat
     1081 attatctttg atttgtattt cattcttatt atttttttct tttatgaata cttttatttc
     1141 actactcttg gaataaaact ccaaagtttg ttctatatat tttatttctt ttccattcat
     1201 attactagaa atttcatttg ccatattatg aatcttttct tttctcgtct ccaaatatgt
     1261 tttaggaaag ataaaaaaaa ttaatgaatg aactaatata attattattc caagaacaga
     1321 aaatatttgt atgaacattt ttggaaatat ctttaatttt ttcattttct ctccaat
//
LOCUS       DAMEET010000191         1374 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.202, whole genome shotgun sequence.
ACCESSION   DAMEET010000191 DAMEET010000000
VERSION     DAMEET010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.202"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            27..788
                     /locus_tag="PB780_002652"
     CDS             27..788
                     /locus_tag="PB780_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDE0734648.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(903..1343)
                     /gene="fabZ"
                     /locus_tag="PB780_002653"
     CDS             complement(903..1343)
                     /gene="fabZ"
                     /locus_tag="PB780_002653"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDE0734649.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          459 a          265 c          261 g          389 t
ORIGIN      
        1 aaatagtaaa ttggaaagga acagttatga agaaaaataa agttatttta agtattagtt
       61 tagcagcagc attgctgttt gctgctggtt gttctgcaaa caacacgact tcttctagtt
      121 cgtctacaga aagttcaaaa agtacatcta ctgcggaaag cacgactgcc agtagcgtcg
      181 atttgaacgc attggaatta ccacaattga cgaaagatgt cgccgaaaat gaagatctgg
      241 tcgaaatggt gacgaataaa ggcacaatca aaatcaaatt atttcccgaa cacgcaccaa
      301 aagcggttga aaactttatg acacatgcaa aagacggtta ctacaacgga ctgactttcc
      361 atcgcgtcat caaagatttc atgatccaag gtggcgatcc taaaggcgat ggcactggag
      421 gggaaagcat ttggggagaa ggatttgaaa cagagatcaa caaccacctt tataatataa
      481 gaggtgcgtt atctatggct cgctcacaag atcctaacag taacggcagc cagttcttta
      541 tcgttcaaaa tacagatgat atgcatgatg gtcttttaaa agacgactat cctcaagcaa
      601 tcatcgacgc ttataaaaac ggcggttatc cttcactaga tggccattat actgtcttcg
      661 gccaagtgat tgaaggaatg gatgtcgtgg atgctattgc aaatatcgaa acagatagtt
      721 cagacaaacc aaaagaagaa gtaaaaatcg ataaaatcaa tatactgcaa gaagcaaaac
      781 aaaaataaaa aaataacgtc tgaatcctgc atagatgaca acgtatgcta ggattcaggc
      841 gtcttctttt tacataaaaa gcaagtaagc ttccttttgt tttgctatat caggttttat
      901 agtcaagcat tttggttgac gatgaacgtg aactcgcact cacagacttt tttatcatct
      961 acatatgctg cagataacgc tgtaccgact gatcccttca ttttggtgat ttcaaaatga
     1021 catttcagca catctcctgg aacaactttt tgacggaatt tggccttatt gatcccaccg
     1081 atataagctc tttttcctag aaactcttct gattttagga taagtatcga acctgcttgt
     1141 gccagtgttt ctagaatcaa cgcacccggc atcactggat tgtcaggaaa gtgaccttgg
     1201 aaaaaatctt cattgattgt tacatttttt gtcgcaacga tcttttttcc tggttctaaa
     1261 gtatctacat aatcaatata acaaatagga taacgattag gaatcaattc cattacttca
     1321 gtagcagata atacgtaaga cataattaaa ctccttcttt caggcaattg ccat
//
LOCUS       DAMEET010000192         1372 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.203, whole genome shotgun sequence.
ACCESSION   DAMEET010000192 DAMEET010000000
VERSION     DAMEET010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.203"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            37..1338
                     /locus_tag="PB780_002654"
     CDS             37..1338
                     /locus_tag="PB780_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDE0734650.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          446 a          275 c          253 g          398 t
ORIGIN      
        1 ttgaatcacc acaacacaat caaaggagcg ttctgtatgc ctgatcttca tcatatcaaa
       61 ctactacttg gaatcaaaga taaaaacatt ttcatcacaa atgtggaatc gaagagcatt
      121 aagaatatca agagtcttgt tgtttctgcg acgttaagta aagaaattcg tcgctgtcct
      181 ttgtgtaaac agatgaatca tgaaggcatg atcgttaaga acggaaagaa aaaatcgctc
      241 attcaactta ataaatgtgc gaaccaactg acttatcttg ctttagcgaa acaacgctac
      301 cactgtcgtg gctgccacac ctattttacg gcgaatacct acattgtcga tagaaactgc
      361 tttatcgcta aacaagtgcg ctataaaatt ttagaagagc taactgaaaa acaagcaatg
      421 acgacaattg ctaagcattg cggcgtttct tggtcgaccg tttctcggac actcgcttct
      481 ctgatcccca tgacaaaagt aaaaagaaat tggttaccgc gttgtttatt aatggatgaa
      541 tttcgttcac tgaaaaatca agtcggtccc tactcctttt cttgtatgga tggcgatact
      601 ggaaaattac tggatattct tccttcacgg aagaaaaagg ctctcgtttc ttattttatg
      661 caatttgaac gacgcgcacg attgaaggta aaggtcatcg tgacggacat gaatgtttct
      721 tatgcctctc tgataaagga atgttttcca aaggcaaaac tcgttgtgga tcgttttcac
      781 attgtcaaac atctcattcg taaatttgag gatattcgcg tacgaatcat gaaaagcttt
      841 gatcgaaacg atccaacaca agccaaatat tatcgacaat taaaggcgtt gtctcggctt
      901 ctgatcaaac gacaggacaa gcttgtttat gacaaatgga ccaaatggcg caattttggt
      961 tgggcgtatc tcaccgaaag tgaagtggtg gatcgtctgc tttcgatctc tgacgaatta
     1021 cgaatcgcct atttttacta tcaagaaatt cttcaagcgt ttcacgacaa agaggcggac
     1081 actttcttta aacttgtaag aacgatgccc aattccgttc caaaagaact gcattctata
     1141 aaaaaagctt ttataaacta tgaatccggc attcgtttag ccctcgaatt gccttattca
     1201 aatgccaaaa tagaaaatct gcatacccac atcaaagcat tgaaacgagt cgcttatggc
     1261 tttcgaagct ttcggaaaat gaagaccaga attttcttac taaataatct gattacctat
     1321 gaatcaaaaa acatctaaag tagaaaagga attctacctt agatgttctt at
//
LOCUS       DAMEET010000193         1364 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.204, whole genome shotgun sequence.
ACCESSION   DAMEET010000193 DAMEET010000000
VERSION     DAMEET010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.204"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(128..526)
                     /locus_tag="PB780_002655"
     CDS             complement(128..526)
                     /locus_tag="PB780_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDE0734651.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(523..759)
                     /locus_tag="PB780_002656"
     CDS             complement(523..759)
                     /locus_tag="PB780_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDE0734652.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            complement(919..1203)
                     /locus_tag="PB780_002657"
     CDS             complement(919..1203)
                     /locus_tag="PB780_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734653.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
BASE COUNT          433 a          240 c          196 g          495 t
ORIGIN      
        1 atttcaaaat taatccatat tttccatatt tttatgaaaa aactattttg tatgattaaa
       61 cataaaaaaa tcagcatatt ccttaatgac atgctgatct aattgagaaa taaagttttt
      121 aattcgttca ttttattgcg gtttgtcgta aataactggt tatcttggac tttagtgaat
      181 cgaaatccaa tgaactagtg gtaatcatta tgataaaatc aacagcttct tgacgagaaa
      241 aagcaactgg acaattattc aattgaagaa acaacaacat ggcaacaaga gcagttcgtt
      301 tattgccatt atgaaaagga tgttttttag ctaaattaat tgctaaaatc gccgcttttt
      361 catagatttc aggatagagt tcttgattga atactgcttg tgaaggctgt ttgacggcca
      421 tatctaatgc atttgcatct tttacaccga tcatttcgcc tggcgagtga cgtaaaataa
      481 ctttcgcgtt gattttgata atttctttag tcgaaagata cctcataaat caaccaaatc
      541 ttttaacgcg tcttcatatt gattcatgat cgaatcaact aatgaatcaa tatcaggttc
      601 ttcttgtgct ttgatcattc gtacagaacc atcagattga aaaacataac tgattgcgtc
      661 acctgtctgc acacccatga attcttttac ttctggtggg atagttgtta catcgctatt
      721 gccagatttt ctaattttat actttttcat ttttttcata tttcgccacc ctctatacca
      781 ttctcattct atagtaagta cattatatcc aagtatatac ttttaatcaa tgtatatact
      841 tttattttgc tttttcttac gaaaaaatga caaagaaaaa agtttgtgac atgacttttg
      901 tcacaaactc tcgtattctt aaccaaaata ttgatgaaat acttgtgtaa taactggtag
      961 aaaataaata atgatcatag ccgctaccac caccaacaat tgttttcgct gtttctttcg
     1021 ctttagcatg cttgtcgttt tatgaaaaaa ataagtagcg cttttagctc ctgcagtcaa
     1081 agtcgataaa atcaacgttg gtgaatatgc tgtttgattg tgcgtcaatg acaacaactt
     1141 cgtcccgtaa tcaggatact ttagaaatgc ttggtacaca gccacaatcg tgattgctcc
     1201 cataagataa tttaaaaatg aatgcacttt tttcaaataa aaaaactcct tgctatctat
     1261 ataaaatcag cctaccccaa cgtttttgct tgattcactg atatttttta gttttattgg
     1321 tatttatatt ctcacttttc ggaataaata ttccgctatt tcat
//
LOCUS       DAMEET010000194         1348 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.206, whole genome shotgun sequence.
ACCESSION   DAMEET010000194 DAMEET010000000
VERSION     DAMEET010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.206"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            82..>1348
                     /locus_tag="PB780_002658"
     CDS             82..>1348
                     /locus_tag="PB780_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HDE0734654.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIF"
BASE COUNT          458 a          260 c          256 g          374 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttt
//
LOCUS       DAMEET010000195         1342 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.207, whole genome shotgun sequence.
ACCESSION   DAMEET010000195 DAMEET010000000
VERSION     DAMEET010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.207"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            161..898
                     /gene="erm(B)"
                     /locus_tag="PB780_002659"
     CDS             161..898
                     /gene="erm(B)"
                     /locus_tag="PB780_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDE0734655.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
     gene            1068..1328
                     /locus_tag="PB780_002660"
                     /pseudo
     CDS             1068..1328
                     /locus_tag="PB780_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
BASE COUNT          514 a          222 c          219 g          387 t
ORIGIN      
        1 aaacagacta aaaacagtga ttacgcagat aaataaatac gttagattaa ttcctaccag
       61 tgactaatct tatgactttt taaacagata actaaaatta caaacaaatc gtttaacttc
      121 tgtatttgtt tatagatgta atcacttcag gagagattac atgaacaaaa atataaaata
      181 ttctcaaaac tttttaacga gtgaaaaagt actcaaccaa ataataaaac aattgaattt
      241 aaaagaaacc gataccgttt acgaaattgg aacaggtaaa gggcatttaa cgacgaaact
      301 ggctaaaata agtaaacagg taacgtctat tgaattagac agtcatctat tcaacttatc
      361 gtcagaaaaa ttaaaactga atactcgtgt cactttaatt caccaagata ttctacagtt
      421 tcaattccct aacaaacaga ggtataaaat tgttgggaat attccttacc atttaagcac
      481 acaaattatt aaaaaagtgg tttttgaaag ccgtgcgtct gacatctatc tgattgttga
      541 agaaggattc tacaagcgta ccttggatat tcaccgaaca ctagggttgc tcttgcacac
      601 tcaagtctcg attcagcaat tgcttaagct gccagcggaa tgctttcatc ctaaaccaaa
      661 agtaaacagt gtcttaataa aacttacccg ccataccaca gatgttccag ataaatattg
      721 gaagctatat acgtactttg tttcaaaatg ggtcaatcga gaatatcgtc aactgtttac
      781 taaaaatcag tttcatcaag caatgaaaca cgccaaagta aacaatttaa gtaccattac
      841 ttatgagcaa gtattgtcta tttttaatag ttatctatta tttaacggga ggaaataatt
      901 ctatgagtcg cttttttaaa tttggaaagt tacacgttac taaagggaat ggagataaat
      961 tattagatat actactgaca gcttccaaga agctaaagag gtccctagcg cctacgggga
     1021 atttgtatcg ataaggggta caaattccca ctaagcgctc gggacccctt gtaggaaaat
     1081 gtcctaagtg tggcaacaat attgtattaa aaaaatcgtt ttatggttgt tcaaattatc
     1141 ctgaatgtaa gtttacttta gctgaacatt ttagaaagaa aaaactaacc aaaacgaatg
     1201 taaaagaatt actggaggga aaagaaaccc tggtaaaagg aatcaaaaac aaagagaaaa
     1261 agccctacaa tgccgttgta aaaattgggg aaaagggata tattgatttt atctcttttt
     1321 caaaataaac ataaaagccc tt
//
LOCUS       DAMEET010000196         1302 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.208, whole genome shotgun sequence.
ACCESSION   DAMEET010000196 DAMEET010000000
VERSION     DAMEET010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.208"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            52..>1302
                     /locus_tag="PB780_002661"
     CDS             52..>1302
                     /locus_tag="PB780_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDE0734656.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          403 a          260 c          252 g          387 t
ORIGIN      
        1 cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat
       61 actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat
      121 tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg
      181 tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg
      241 gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag
      301 ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa cattcgtagc ggatacctct
      361 gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta
      421 aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc
      481 tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac tttgccaaag
      541 gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat gagttttatc
      601 atgatggacg gacaaagcca tgcattattg gatattgtcg aaaatcgacg cttgccttat
      661 ttagaacgct atttttctcg gttttcatta gcgactcgtg aagccgttca attgattgtt
      721 attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg
      781 atcatcgatc gtttccacat cgtccaacat attggcagaa ctttcttgaa tcatcgagtg
      841 aaagaaacca cggtacgact taaaggaaat tcccactctc aacgagggtt agggaaaaaa
      901 ttgaagcgct attggaaact cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg
      961 gtttggcgtg ccagttttaa agattatttg actgaaagtg agattgccga tcgactgtta
     1021 gctgcttgtc ccaacttacg ccaaggctat cagttatacc aagacattct ttatgcggta
     1081 aagaaaagag atcagtcact atttgaggat tgtttaacta gagacattag cggtctgcca
     1141 gaaacctata caaccacttt acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg
     1201 cttcactaca gctattcaaa cggaccattg gaatgcttaa acaaccacat caaagtatta
     1261 aaacgcaatg cgtacggctt tcgcaacttt tacaatttca aa
//
LOCUS       DAMEET010000197         1276 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.209, whole genome shotgun sequence.
ACCESSION   DAMEET010000197 DAMEET010000000
VERSION     DAMEET010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.209"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(31..1203)
                     /locus_tag="PB780_002662"
     CDS             complement(31..1203)
                     /locus_tag="PB780_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HDE0734657.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
BASE COUNT          345 a          265 c          245 g          421 t
ORIGIN      
        1 taaaatacta tacaatgttt taccggtctc tcattgatca aacttaatat attttcttgt
       61 agaactgagc cattcgtcat gaaggtccat aaggacagct ccaattaatc gattggcaga
      121 cgttcggttg ggaaaaatcc gaataatctt ttctcttctg cggacttcct ggttcagtcg
      181 ttcaagaagg ttggtgcttt ttagccgatt atgactattt ccgataaccg tgtattgaaa
      241 ggcatcttcg aagccattat ccaatatttc gcaggctttt gtatatttag gctggtcgat
      301 atattcaccg actaaggcat tcttagctgt tcgagcgagt tcaatatccg taaacttaaa
      361 gatcgctttt actgcttctc taaacggttt tgaattcttt tttggaatgg aactgaagat
      421 gtttcttaaa aaatggacct ggcatctctg ccaacttgcg ttggtaaatg acttacgaat
      481 cgcagacact aggcctttat gggcatcaga aatgatgagt tctgtcccct ttaggccgcg
      541 ttcttttaag tattcaaaga agatggacca tgtgtcatca ctttcttcat tttgaatcat
      601 gaagccaatg atttcacggt cgccaccttc tgttatcccg atcgcaatat ggcagctttt
      661 agaaagcact cgatggtcct cacggacttt tatgtagaga acatcagtca tcagataagg
      721 ataattcgta cctgagagtg aacggttctg ccattcgttg accatcgggt ctaactgctc
      781 agtcaggcta gaaacaaaag atttcgatac agattttcca catagctctt caacaatctt
      841 tgaaacttta cgtgtcgaaa cgcccgaaac atacatctca agcattgaag cgagcagtgc
      901 cttttcattt cgctgataac gctcaaacac cgtcggtgaa aattcaccat cacgtgttct
      961 aggcactttt aattcgagtg tacccacacg agtcgtaaag tctcgctcat aatagccatt
     1021 tctttgactc tgacgacttt ctgaacgttc atagtcatcg gcttgaatat attctgttcg
     1081 ttgattttcc atcaattggt tgaaaacagt ggttaaaata tttttagaaa catcatcttt
     1141 taccgaatgt tcaataatac tttgaacctc ttcgttgttc agtgtaaaat gtacttgggt
     1201 catgtaacgt cctcctgggt atgtttttgt ggttaaaaac attgtaccgt aaaagggctg
     1261 ttacatggcc ttttat
//
LOCUS       DAMEET010000198         1274 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.210, whole genome shotgun sequence.
ACCESSION   DAMEET010000198 DAMEET010000000
VERSION     DAMEET010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.210"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(126..602)
                     /locus_tag="PB780_002663"
     CDS             complement(126..602)
                     /locus_tag="PB780_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDE0734658.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(615..>1274)
                     /locus_tag="PB780_002664"
     CDS             complement(615..>1274)
                     /locus_tag="PB780_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDE0734659.1"
                     /translation="RAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYV
                     IHAFLDGLSFYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFI
                     YFFVFTFFIKKFKVDIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVE
                     ACATRLRVAVTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKD
                     N"
BASE COUNT          451 a          236 c          167 g          420 t
ORIGIN      
        1 ggcatgggga atcattacta ataaaataaa aaaatgaaca gaaagaaaac agttctttct
       61 gttcaccaac ttcatttgac aaatatccta tttagcttat tttatagctt tttatataca
      121 cttttttaaa aagtgatttt tccacatgaa ttcccatgag caattcgacc attatctatt
      181 attaaatcat tgaactgatt tttctccgga aaaacaacta taacagaagt atctttacca
      241 gattttatta ttttgtcaaa atccacatca accaacgagg tttcaggagt aacctcatca
      301 ttttcttgaa cacaaatcga aaaaggctct ccttgtaatt caactgtatc aattccaata
      361 tgaatcaaat aatcaatgcc tgagttgctt tttatattaa tagcatgttt agtaggaaat
      421 atatttgtta ctcgaccagc caatggagca taaatttttg aaccatcagg tatgacggca
      481 aaaccatcac ctagtaattt ttgtgaaaag acaggatcat ttacttctgt gatggaaatg
      541 atttttccgg aacaaggtgc ataaaaagtt tcttgttttt tctttttgaa aatttcaaac
      601 atattaaaaa cctcctaatt atcttttcct aatatttgat tgatttcttg acttaataag
      661 tcagctttcc ctccaaaaac agcttggatg ccacccttca cttcgaagac agcagttgca
      721 ccagtgtctt ttattgtttg tttttgaata atctccccat tcgtaacagc aacacgcaaa
      781 cgagttgcac aagcttcaac agactcaata ttttcttttc caccaagagc ttcaataatg
      841 ataagagact cttcatgtaa tgatgtattt ttgtttatta ctttttcaga atgaacttct
      901 gattcttctt ccattccagg aatatctacc ttaaattttt tgataaagaa tgtaaaaaca
      961 aagaaatata taaatgccca tgcaatacca aaaggaatta attttaacca attagttttg
     1021 tcattccctt gaagtacacc gaataataaa aagtcgatca aaccacctga aaaggaattc
     1081 cctacacgaa tatttaagat atcagcaaaa taaaaagata atccatctaa gaatgcatga
     1141 atcacataaa gccaaggagc aacaaataaa aacatatatt caactggttc agtaattcca
     1201 gttaaaaatg atgtaagggc cccgctaaaa taaagcccac cattcttttt acgatttttc
     1261 ttaggaattg cacg
//
LOCUS       DAMEET010000199         1262 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.211, whole genome shotgun sequence.
ACCESSION   DAMEET010000199 DAMEET010000000
VERSION     DAMEET010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.211"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1217)
                     /locus_tag="PB780_002665"
     CDS             complement(<1..1217)
                     /locus_tag="PB780_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDE0734660.1"
                     /translation="MQTFLTKTSFKKVVLLDYLLEKDNWCAMEELRNLLNVTEKSVLH
                     YIEELEDLFKQYNGNILLKNEDNKRFFIKKEKDFPIYNIYLHFYKASYNYHLIDFMYK
                     YPRSVLKDFAKEQFTSVSTVFRYAKLLIPYFRRYHITFHPFQLELNASEANIRSFFYY
                     FYWNSTRESSDKWPFHIEQKEIEKYIVAFEGIYDITLTIFQKRVFSFWLAINIERSSF
                     RKVRVDNEYKSVISDDPHFNLLKKWSKQINLSFNSDELCFLYRIIYSFGVIDGNAIYE
                     NSHAYAHQRQNTCSYRAVENLEKVLQSMFRFSLDIKDPELIFNFIAFHERSYLFYGNP
                     DLFFNRSYIEEMKEEEPRTYHIMEKLKKELQANADLDVSKKLENWAQLFLDYYYVLDY
                     YDLFLTNVKPIKI"
BASE COUNT          460 a          197 c          146 g          459 t
ORIGIN      
        1 aaaatcttaa tcggtttaac atttgttaaa aataaatcat aataatctaa cacataataa
       61 taatccaaaa ataattgtgc ccagttttct aatttctttg atacatccaa atccgcattt
      121 gcttgtaatt cttttttgag tttttccatt atgtgataag ttcgaggttc ttcttctttc
      181 atttcttcga tatacgaacg attaaaaaaa agatctggat taccataaaa tagataactt
      241 ctttcatgaa atgcaataaa attgaatatg agttctggat cctttatatc taaagaaaaa
      301 cggaacatgc tttgtaaaac tttctccaaa ttctcaactg ctcgataaga acaagtattt
      361 tgtcgctgat gtgcatatgc atgactattt tcatatattg catttccatc aattactcca
      421 aaagaataaa ttattcgata aaggaaacac agttcatcgc tgttaaaaga aagattgatt
      481 tgttttgacc acttcttcaa aagattaaaa tgcggatcat ctgagataac tgatttatat
      541 tcattatcta ctcgtacttt tctaaatgaa cttctttcta tattaatggc taaccaaaaa
      601 gaaaatacac gtttttgaaa aatagttaac gtaatatcat aaatcccctc aaaagcaact
      661 atatacttct ctatttcctt ttgttcgata tgaaatggcc acttatcact actttcacgt
      721 gtagaattcc aatagaaata ataaaaaaag ctacgaatgt ttgcctctga tgcattcaat
      781 tctaactgaa atggatgaaa tgttatatga taacgcctaa aatatggaat taagagcttc
      841 gcatatctga aaacagtaga tacactagta aattgctctt tagcaaaatc ttttagaaca
      901 cttctaggat atttatacat aaaatcaatc aaatgataat tataagaagc tttataaaaa
      961 tgaagataaa tattataaat aggaaaatct ttctcttttt ttatgaaaaa tcgtttatta
     1021 tcttcatttt tcagaaggat attaccatta tattgcttaa ataaatcctc tagttcttct
     1081 atatagtgta agactgactt ctccgtaaca ttaagtaaat ttctaagttc ttccattgca
     1141 caccaattgt ctttttctaa caaataatct aataaaacta cttttttgaa agatgtttta
     1201 gttaaaaaag tttgcatcat ttcacctcaa taagttactt ttgtttattt taatcaaaga
     1261 at
//
LOCUS       DAMEET010000200         1239 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.212, whole genome shotgun sequence.
ACCESSION   DAMEET010000200 DAMEET010000000
VERSION     DAMEET010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.212"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..988
                     /locus_tag="PB780_002666"
                     /pseudo
     CDS             1..988
                     /locus_tag="PB780_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          434 a          222 c          255 g          328 t
ORIGIN      
        1 atcttacgcc tgaacaaaaa gaattgttag aactacgtaa aagaaaccag caattagaaa
       61 tggaaaatga tattttaaag caagcagcgc tgatattcgg acgaagagac aagtaatcga
      121 tgcgaataag catctttacc ctatatcagc gatgtgcaga atattaggtc tatcacgtca
      181 gtcctattat tatcaatcaa aaccaaagaa agacgaatca gaacttgaag aagtagtcgc
      241 tgaagaattt atccgcagcc gaaaggccta cggctcaaga aaaataaaaa aagccttatc
      301 aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa atagaggatt
      361 aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca atgaagccaa
      421 aacgacaaac gtattgaatc gtaaattctt aagagacaac ccattagaag cgatcgtaac
      481 agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca ttttggatct
      541 gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg ttctagtaaa
      601 aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc atactgatcg
      661 tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg acatcaatcg
      721 atcattgagt cataaaggct gtccttttga taatgccgta gctgaatcaa cttataagtc
      781 gttgaaagta gaatttgtct atcaatacac atttgaaacc ttacaacaat tggatttgga
      841 gttatttgac tatgtcaatt ggtggaacca ccttcggttg cacggtacac ttggctacga
      901 gacaccggtt ggttaccgta accagagatt ggcgcagcga atccttgata atgagctcgg
      961 atgtgctaac gctagcgagg cagtctaact ttaactgtta gctcctgccg aagatcgtca
     1021 catccgagga ggctcattgt caaggacaat cggaatagca tacggaaaga agttctgcac
     1081 cttataaaat ttgtcaaaaa aactgttgcc attccaatga gaaaaagcat ggaatgcgct
     1141 gcatcaggta tcgaggctta gaaaaaaatc aggtgcatac gatgcttgtt tttgccgaaa
     1201 tgagtctgaa aaaattggtc acttagcagt gggagcata
//
LOCUS       DAMEET010000201         1215 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.213, whole genome shotgun sequence.
ACCESSION   DAMEET010000201 DAMEET010000000
VERSION     DAMEET010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.213"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            414..947
                     /locus_tag="PB780_002667"
     CDS             414..947
                     /locus_tag="PB780_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HDE0734661.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DAMEET010000202         1213 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.214, whole genome shotgun sequence.
ACCESSION   DAMEET010000202 DAMEET010000000
VERSION     DAMEET010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.214"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(74..1168)
                     /locus_tag="PB780_002668"
     CDS             complement(74..1168)
                     /locus_tag="PB780_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDE0734662.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT          354 a          259 c          192 g          408 t
ORIGIN      
        1 tataaaaaaa caaaataaaa aagcagaaat aactggctat ttctagcgga ttatttctgc
       61 aataactaat tatctatttt ttattttcaa ttaatttaaa gcttgaccat ggtttcaaaa
      121 agtcaaggag gaataattcg tcatcggaga ttcttcctac aacattgaca cgtccgtcgt
      181 ttttcatatc cttcaaggcg atttgtgttt ctcctttgta ttgaccgtac ccttcattgt
      241 caatcagtac atccccatga tgcatatctc gcgtattatg aggcgggaaa tctttatctt
      301 tgtagtatac acgagtcatc gttgaacgaa ggatatattc tgagcgatcc cctcgataac
      361 tatgaaggtt atcaaacaag cagattcgtt cgttttctgt aataccttct tccgtatcta
      421 ctttcaatgt tggataatcc gcgttaaatg cttctgccat ttctttcaat tcttcttcac
      481 ttgcgtacgc atttccaata gaaatatcat caatcaatcc tgtaaggatt aaatgcttca
      541 cttgtgtcgc aatttcaaga tctcggtggt cttccagcgt gcacaagcca tcttgcgtag
      601 gccaagggcc aaactcagca gattgcgaat tgacgaatgc catcgtattc aaattatatt
      661 ttctgaattt ttctgaacaa aaaacaaagt gttcgtatcc taaaccagag tatcgatgag
      721 gataaaaatt atgagaacct aataaattat ccgtgtttgg cgaataactc atgatgttgt
      781 caacgtaatt cgtgcccgaa ctcatattga tctcgatttt gataccaaaa ggattgcgcg
      841 tcatttttgc ttcttctgct cctgtgaatc cgatatctaa gcggaccccg taggcaccca
      901 tcttatggaa aaaagataag tcatcgtaag aaatctccaa ctgttcaaat aacgctggat
      961 tgatatcaac cattacttcc atgcctaggc tattcgcgta atcaactact tctttaaatt
     1021 ctgaaagaac tttttcttta tcatcattga tctgcagcaa actggtaaaa acccgtttat
     1081 acccatactt atgagctaaa tccaagtacg ctttgtcctt ttcaaatgtt gaccgttctg
     1141 gataaatcga gattcctaat tttcccatat taccatttcc tcctgcatct tttcgttttt
     1201 gtatccactt aca
//
LOCUS       DAMEET010000203         1193 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.215, whole genome shotgun sequence.
ACCESSION   DAMEET010000203 DAMEET010000000
VERSION     DAMEET010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.215"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            143..400
                     /locus_tag="PB780_002669"
     CDS             143..400
                     /locus_tag="PB780_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734663.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(632..1063)
                     /locus_tag="PB780_002670"
     CDS             complement(632..1063)
                     /locus_tag="PB780_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HDE0734664.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEK
                     LLYITKTSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          455 a          151 c          148 g          439 t
ORIGIN      
        1 ttttctgtcc acgttcattt tactccaaag ttttcctcat ttcaaaagaa aatcacagaa
       61 tacacgaaga agcgtttcta tttgtataaa ctatatcaaa gttctatact taaattatca
      121 aataaaaagg acgtgaagat aaatgtcaaa tttagaaaac aaagaagaaa aagttgtaaa
      181 caaaattgtt tctgttgtca ataaactgga taaagagtta gacgaacttg atacgttatc
      241 tgaaaatcca gaaaaaaaac ataacctaaa aaaatggtta gttgaacgaa aggctattca
      301 tgaaattaaa aaagttcttc atgaagctga taaatatgaa aaatatgatg aaaaagaact
      361 tgataaagaa tttaaagaaa tcaatgactt attactataa ttgatttata cgactcattt
      421 ttgagtcgtc agactgcaaa caacactcta aatttaggag tttgtctaca gtctgaataa
      481 attttaaatt tattgtccat aatgttatta tgaatttata gcattaagaa aagagataag
      541 actttacatt tttttaggaa tgtaaagtct tatctctttt cttaattgta caattagtat
      601 tatctgcact actttctaat taaataaatt tttagttatt aacttgatta aaatcagaga
      661 taatatcatt taattcagtt tgaagggttg taacgattaa atggaaattt tcttcatctg
      721 ttttattata tggggatgtt ttcgttatat ataacaactt ttctttttca attttcatta
      781 ataactctgt cgaagtctgt actttatctt gataattaaa tgcagacata ccactttgta
      841 taattgtaat tattgctgaa gcaattgaaa caatcaatag taaagacctg tgttcggaag
      901 cctgatcaat taatattggg atactagctg ataagactat ttttataata tttccgatac
      961 caattactct attataaaat cgaatttctt tttttaatct atcgattgta gaatcaatag
     1021 attcgataaa cgctttttga tccatcattt tctctccaat cattttatta aaatcattaa
     1081 caaaaatatt ataataggat ttaagtaagt tctaactaat cacatataaa aataaaaaaa
     1141 catttgtatt ttatcaaagt tttattatac ttgtcaattt gtgtacatcc taa
//
LOCUS       DAMEET010000204         1085 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.217, whole genome shotgun sequence.
ACCESSION   DAMEET010000204 DAMEET010000000
VERSION     DAMEET010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.217"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            251..349
                     /locus_tag="PB780_002671"
                     /pseudo
     CDS             251..349
                     /locus_tag="PB780_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          383 a          176 c          188 g          338 t
ORIGIN      
        1 gtgaagatga gcagttggcg gtcttttaaa aatccccttt taaggggcag cgagtgtcga
       61 ccccttctag ggggattaaa aatacacccg tcctcacggg tgtatgggtt actgatagac
      121 ttgattttaa ctggtgtcag aaacttatgt ccagaaaggg tgatgaaatt ttggcaattt
      181 acggctattc aagaattagc acaagcaacc aagattacaa aacacaaatt caaacattag
      241 aaaatgctgg cgcagaaata atcttcctag aaaatcactg gattatcgaa cacctttgga
      301 agtatttttg agttacgtaa gtattgatga tctgtctaac ttaatttgac aattaagagt
      361 atatttactt cagatggtaa ggcaacaaaa taaaatacaa taggcagatg aactaataaa
      421 taaaatctgt attaaataca gataaattaa tataaaaatc aaaaggatca taaactttta
      481 acaagtgatg aaatgggtgg aaattgattc ttatagacag tttgtctcat aacattgaag
      541 tattttgcaa ttttagcatt gaaacacttt tttaatcttc aacgttgttc ttataaacaa
      601 gatgattggg aatcttttga attaacactg tatagcttag aacacccttt atagaaaaca
      661 ttcgcttagc aatcttaata attccttgtg cccgatgtcg tttagattca gtaggttcct
      721 gctcatcgat catggaaaaa atttgaagga tcagatcctt aataaattta tttaagagag
      781 gatgatccat tgcttcatcc atactcgata aactagtgat aatgagctac acgtttttct
      841 ttcaagttga tcaatagctt ccacgataaa aatatcttta gataaaatca agaaccgtag
      901 gaactacagg gatatttggt caataagagc caccgctatt agtaaaaaat aagctatcaa
      961 gtatgctctc atcccaagaa ttgcctactt caatcgttaa aaaccgttag gtttagctaa
     1021 gtgggggtag ttcactcaat acttttatca ataatagagc gcaaaacttt ttctcttaat
     1081 tcaac
//
LOCUS       DAMEET010000205         1085 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.218, whole genome shotgun sequence.
ACCESSION   DAMEET010000205 DAMEET010000000
VERSION     DAMEET010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.218"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..824
                     /locus_tag="PB780_002672"
     CDS             <1..824
                     /locus_tag="PB780_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDE0734665.1"
                     /translation="FKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMA
                     SGDEMLTYAYTPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISP
                     IEKGFETGKYAMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMT
                     EKTEKKDASAAFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQ
                     LSGHARPVVVAYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            867..>1085
                     /locus_tag="PB780_002673"
     CDS             867..>1085
                     /locus_tag="PB780_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDE0734666.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFV"
BASE COUNT          355 a          191 c          237 g          302 t
ORIGIN      
        1 tgttcaaaga tgctggaatt acagaagatc aattgccgac gttagaagat ccttggactt
       61 ggacggaatt tcaagcaatt tgccaaaagc tgactgataa atacaaaacg cctgcaattg
      121 atatgcaaat ggcgtcggga gacgaaatgc tgacctacgc atatacacca tttatatggt
      181 cgaatggcgg cgatgtcgtt aatgctgatg gaaccaaagc cgaagactat tttaacggta
      241 aaaaatccgt tgaagcgttg gaatttattc aagaattggt tgaaaaaggt tacaccacaa
      301 tcagtcctat tgaaaaagga tttgaaactg gcaaatatgc catgatgctg agcggttctt
      361 ggacaatggc agatttagag gaaaactatc aagagattga ttatggtatt ttgccttacc
      421 ccgtttcaga tgcaactggg gagttagttt caccaaccgg aagctggcag ttagcaatga
      481 ctgagaaaac agagaaaaag gatgcttctg cggcctttat taagtttgca accagtacag
      541 aatctagtcg tattatgagt ttagggaaca gtgttctacc tatcagaaat tcaacagttg
      601 atttaattaa agatgaagtg tcggaaggaa tgcaggtatt aattaagcaa aaccaacttt
      661 ctggacatgc ccgcccagtg gttgtagctt atccgcaagt atctagagca ttccaacaag
      721 cagttcaaga tattagctac ttcgaggaaa acccagatgt acaaaaagta gcagatagtc
      781 gcgctgctga aatgcaacag gcaattgata cttcactaaa atagaagttt ctaaaggggg
      841 ccaatcaagt gttaaaaaaa ccgacaatga aagacaattt gataggttac ggctttctag
      901 cgccagcttt gctgctgctg acgatttttc tgatcatacc cgtaatcatg gtaatttatt
      961 atgcctttac tgattattat ttgttaacgc cagatgctag acaatttatc ggcttagaaa
     1021 attttaagcg actgttcgct gatccaattt ttgtaaaaag tatttggaac acggctaaat
     1081 ttgtt
//
LOCUS       DAMEET010000206         1081 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.219, whole genome shotgun sequence.
ACCESSION   DAMEET010000206 DAMEET010000000
VERSION     DAMEET010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.219"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(48..132)
                     /locus_tag="PB780_002674"
     tRNA            complement(48..132)
                     /locus_tag="PB780_002674"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(96..98),aa:Leu,seq:caa)
     gene            complement(153..223)
                     /locus_tag="PB780_002675"
     tRNA            complement(153..223)
                     /locus_tag="PB780_002675"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(189..191),aa:Cys,seq:gca)
     gene            complement(269..340)
                     /locus_tag="PB780_002676"
     tRNA            complement(269..340)
                     /locus_tag="PB780_002676"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(306..308),aa:Gln,seq:ttg)
     gene            complement(352..424)
                     /locus_tag="PB780_002677"
     tRNA            complement(352..424)
                     /locus_tag="PB780_002677"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(389..391),aa:His,seq:gtg)
     gene            complement(427..500)
                     /locus_tag="PB780_002678"
     tRNA            complement(427..500)
                     /locus_tag="PB780_002678"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(466..468),aa:Trp,seq:cca)
     gene            complement(507..587)
                     /locus_tag="PB780_002679"
     tRNA            complement(507..587)
                     /locus_tag="PB780_002679"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(551..553),aa:Tyr,seq:gta)
     gene            complement(589..664)
                     /locus_tag="PB780_002680"
     tRNA            complement(589..664)
                     /locus_tag="PB780_002680"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(629..631),aa:Phe,seq:gaa)
     gene            complement(700..772)
                     /locus_tag="PB780_002681"
     tRNA            complement(700..772)
                     /locus_tag="PB780_002681"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(737..739),aa:Asp,seq:gtc)
     gene            complement(782..854)
                     /locus_tag="PB780_002682"
     tRNA            complement(782..854)
                     /locus_tag="PB780_002682"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(819..821),aa:Val,seq:tac)
     gene            complement(870..941)
                     /locus_tag="PB780_002683"
     tRNA            complement(870..941)
                     /locus_tag="PB780_002683"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(906..908),aa:Glu,seq:ttc)
     gene            complement(956..1044)
                     /locus_tag="PB780_002684"
     tRNA            complement(956..1044)
                     /locus_tag="PB780_002684"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1008..1010),aa:Ser,seq:gga)
BASE COUNT          303 a          284 c          246 g          248 t
ORIGIN      
        1 ttatttaatt tctcaataaa aaactaagat gaagacatct tagtcaatac cggcggccgg
       61 ggtcgaaccg gcacgccctc aacgggcact ggattttgag tccagcgcgt ctgccaattc
      121 cgccacgccg gcaagttgat ttattagtac taaggcggta accggatttg aaccggtgat
      181 gaaggttttg cagacctctg ccttaccact tggctatacc gccattatac tattatcaat
      241 ctacaaaata aagaataaat tatagaaact ggggtagctg gattcgaacc aacgcatgag
      301 ggagtcaaag tcccttgcct taccgcttgg ctatacccca agaataaaaa gggcgaatga
      361 tgggaatcga acccacgaat gccggagcca caatccggtg cgttaaccac ttcgccacat
      421 tcgccatggc aggggcagca ggaattgaac ccacactaac ggttttggag accgctgttc
      481 tacctttaaa ctatgcccct ataaaatgga ggagagtgga ttcgaaccac tgaaccctaa
      541 ggaacggatt tacagtccgt cgcgtttagc cacttcgcta ctcctccatg gtggcgcggg
      601 acagaatcga actgccgaca cacggagctt caatccgttg ctctaccaac tgagctaccg
      661 cgccatattt taaaattatt aattgaataa caagaacgac ggttccgacg ggaatcgaac
      721 ccgcgatctc ctgcgtgaca ggcaggcatg ttaaccccta caccacggaa ccagttcatt
      781 atggaggtta acgggatcga accgctgacc ctctgcttgt aaggcagatg ctctcccagc
      841 tgagctaaac ctccaacatt caattaaaat gacccgtacg ggattcgaac ccgtgttacc
      901 gccgtgaaag ggcggtgtct taaccgcttg accaacgggc caggtaaatt tgctacggag
      961 agtaagggat tcgaaccctt gagacagttt gcaccatcta catgatttcc aatcatgctc
     1021 cttcggcctc tcggacaact ctccagagtg aaagaagctc aatactcttt ctcaaaatga
     1081 g
//
LOCUS       DAMEET010000207         1079 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.220, whole genome shotgun sequence.
ACCESSION   DAMEET010000207 DAMEET010000000
VERSION     DAMEET010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.220"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            4..978
                     /gene="bsh"
                     /locus_tag="PB780_002685"
     CDS             4..978
                     /gene="bsh"
                     /locus_tag="PB780_002685"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HDE0734667.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          379 a          135 c          186 g          379 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagttact gttactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tgggctaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaaactaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgatgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg
      901 aataaagaag acttagatag tcataagtta attagttatc caattataga aaaacaacaa
      961 attaaatata taaattagtt aatgtgttgt gattgattat ttaatataga tataatacaa
     1021 aaaggcaaag atattttcaa aacatttttt cttgatgtga tatccttgtc ttttttgta
//
LOCUS       DAMEET010000208         1070 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.221, whole genome shotgun sequence.
ACCESSION   DAMEET010000208 DAMEET010000000
VERSION     DAMEET010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.221"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            25..>1070
                     /locus_tag="PB780_002686"
     CDS             25..>1070
                     /locus_tag="PB780_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDE0734668.1"
                     /translation="MTELGSVDKGTTRTDNTLLERQRGITIQTGITSFQWENTKVNII
                     DTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPTIFFINKID
                     QNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEGNDDLLEKY
                     MSGKSLEALELEQEESIRFQNCSLYPVYHGSAKSNIGIEQLIEVITNKFYSSTYRKKS
                     ELCGNVFKIEYSEERQRLAYVRLYGGILHLRDSVRISEKEKIKITEMYTSINGELCKI
                     DKAYSGEIVILQNEFLKLNSVLGDTKLLPQRERIENPLPLLQTTVEPSKPQQREMLLD
                     ALLEISDSDPLLQY"
BASE COUNT          386 a          169 c          223 g          292 t
ORIGIN      
        1 agcttattat ataacagtgg agcgattaca gaattaggaa gcgtggacaa aggtacaacg
       61 aggacggata atacgctttt agaacgtcag agaggaatta caattcagac aggaataacc
      121 tcttttcagt gggaaaatac gaaggtgaac atcatagaca cgccaggaca tatggatttc
      181 ttagcagaag tatatcgttc attatcagtt ttagatgggg caattctact gatttctgca
      241 aaagatggcg tacaagcaca aactcgtata ttatttcatg cacttaggaa aatggggatt
      301 cccacaatct tttttatcaa taagattgac caaaatggaa ttgatttatc aacggtttat
      361 caggatatta aagagaaact ttctgccgaa attgtaatca aacagaaggt agaactgtat
      421 cctaatatgt gtgtgacgaa ctttaccgaa tctgaacaat gggatacggt aatagaggga
      481 aacgatgacc ttttagagaa atatatgtcc ggtaaatcat tagaagcatt ggaactcgaa
      541 caagaggaaa gcataagatt tcagaattgc tccttgtacc ctgtttatca tggaagcgca
      601 aaaagcaaca tagggattga gcagcttata gaagtgataa cgaataaatt ttattcatca
      661 acatacagaa agaagtctga actttgcgga aatgtcttca aaattgaata ttcggaagaa
      721 agacaacgtc ttgcatatgt acgcctttat ggcggaatcc tgcatttgcg ggattcggtt
      781 agaatatcgg aaaaggaaaa aataaaaatt acagaaatgt atacttcaat aaatggtgaa
      841 ttatgtaaaa ttgataaggc ttattccggg gaaattgtta ttttgcaaaa tgagtttttg
      901 aagctaaata gtgttcttgg agatacaaag ctattgccac agagagagag aattgaaaat
      961 ccgctccctc tgctgcaaac aactgttgaa ccgagcaaac ctcaacaaag ggaaatgtta
     1021 cttgatgcac ttttagaaat ctccgacagt gacccgcttc tacaatatta
//
LOCUS       DAMEET010000209         1062 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.222, whole genome shotgun sequence.
ACCESSION   DAMEET010000209 DAMEET010000000
VERSION     DAMEET010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.222"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            51..740
                     /locus_tag="PB780_002687"
     CDS             51..740
                     /locus_tag="PB780_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDE0734669.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            754..>1062
                     /locus_tag="PB780_002688"
     CDS             754..>1062
                     /locus_tag="PB780_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDE0734670.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     S"
BASE COUNT          367 a          132 c          229 g          334 t
ORIGIN      
        1 tttttggagt tattatccaa aaataataaa aaattggagc gtttaggata atggataaaa
       61 agaggttttt agatcttatt tcaaaaggtt tgattgtttc atgtcaagca ttacctggtg
      121 aaccatttta caaagaggaa gaaagtttaa tgccttattt tgctttggct gctcaaaggg
      181 ctggagcagt tggtatacgt gcaaattctg ttcgagatat tattgaaatt agaaagtgtg
      241 tatcattacc aatgattgga attattaaaa gagattatcc accagaatct ccattcatta
      301 ctgccactat gagagaagta gatgaattag tagaaaccgg tgttgaggta atcgctcttg
      361 attgcactct tagaaatcgg catgatggac tatctataaa ttcttttatt actaaaataa
      421 aagaaaaata tcctcatcag ttatttatgg cagatatttc tacatttgaa gaaggcaaaa
      481 atgcatataa agcaggaata gattttgtag gaactacttt aagtggatat actgaggaaa
      541 gtaaaaaaca agatgggcca gatatagatt tgattaaaaa attggtagaa gaagggattt
      601 gtgtaattgc tgagggaagg attcatactc cagaacaagc aaaagagata aaaaaaatag
      661 gagttgcagg tattgttgtg ggtggtgcta ttactcgacc acaggaaatt gcatcaagat
      721 ttgttaaagc attaagttag ggggagaaat aaaatgttta aaaaattttc acaattagga
      781 cgcgctttca tgttaccaat tgctatatta ccagtagccg gattactatt gggactggga
      841 ggagcgttaa caaatgagag tgctataaat gcttatccat ttttagatca accgtggttg
      901 catacaattt taagtattat gagttatgct ggaaatgcag tttttgcaaa cctagcacta
      961 atttttgcaa ttggtattgc tgtaggactt gcaaatggag ataaaggaac agctggttta
     1021 gctggtggag tagcttatct agtatatact gcaactatta gt
//
LOCUS       DAMEET010000210         1059 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.223, whole genome shotgun sequence.
ACCESSION   DAMEET010000210 DAMEET010000000
VERSION     DAMEET010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.223"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(37..993)
                     /locus_tag="PB780_002689"
     CDS             complement(37..993)
                     /locus_tag="PB780_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734671.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgt
//
LOCUS       DAMEET010000211         1001 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.224, whole genome shotgun sequence.
ACCESSION   DAMEET010000211 DAMEET010000000
VERSION     DAMEET010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.224"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>1001)
                     /locus_tag="PB780_002690"
     rRNA            complement(<1..>1001)
                     /locus_tag="PB780_002690"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          210 a          311 c          227 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc g
//
LOCUS       DAMEET010000212          991 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.225, whole genome shotgun sequence.
ACCESSION   DAMEET010000212 DAMEET010000000
VERSION     DAMEET010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.225"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(21..94)
                     /locus_tag="PB780_002691"
     tRNA            complement(21..94)
                     /locus_tag="PB780_002691"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="PB780_002692"
     tRNA            complement(108..181)
                     /locus_tag="PB780_002692"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="PB780_002693"
     tRNA            complement(209..281)
                     /locus_tag="PB780_002693"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="PB780_002694"
     tRNA            complement(301..374)
                     /locus_tag="PB780_002694"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
     gene            complement(384..457)
                     /locus_tag="PB780_002695"
     tRNA            complement(384..457)
                     /locus_tag="PB780_002695"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(421..423),aa:Arg,seq:acg)
     gene            complement(468..553)
                     /locus_tag="PB780_002696"
     tRNA            complement(468..553)
                     /locus_tag="PB780_002696"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(517..519),aa:Leu,seq:taa)
     gene            complement(570..641)
                     /locus_tag="PB780_002697"
     tRNA            complement(570..641)
                     /locus_tag="PB780_002697"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(607..609),aa:Gly,seq:gcc)
     gene            complement(653..725)
                     /locus_tag="PB780_002698"
     tRNA            complement(653..725)
                     /locus_tag="PB780_002698"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(690..692),aa:Thr,seq:tgt)
     gene            complement(743..824)
                     /locus_tag="PB780_002699"
     tRNA            complement(743..824)
                     /locus_tag="PB780_002699"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(788..790),aa:Leu,seq:tag)
     gene            complement(832..904)
                     /locus_tag="PB780_002700"
     tRNA            complement(832..904)
                     /locus_tag="PB780_002700"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(869..871),aa:Lys,seq:ttt)
     gene            complement(908..980)
                     /locus_tag="PB780_002701"
     tRNA            complement(908..980)
                     /locus_tag="PB780_002701"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(945..947),aa:Val,seq:tac)
BASE COUNT          260 a          283 c          235 g          213 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat taacgcgccc aagaggagtc gaacccctaa ccttttgatc
      421 cgtagtcaaa cactctatcc agttgagcta tgggcgcata tttgaaatgc cgaggaccgg
      481 aatcgaaccg gtacggtgat cactcaccgc aggattttaa gtcctgtgcg tctgccagtt
      541 ccgccacccc ggcgcgattg ctttggcaaa gcggaaaacg gggttcgaac ccgcgacccc
      601 caccttggca aggtggtgct ctaccactga gctatttccg cgtgtttaaa gatgccggct
      661 aaaggacttg aaccctcgac cctctgatta caaatcagat gctctaccaa ctgagctaag
      721 ccggcatgta aaacttaaat tatgcgggtg aagggacttg aacccccacg ccgtaaggcg
      781 ctagatccta aatctagtgc gtctgccaat tccgccacac ccgcaaaaat atgagccgta
      841 cagggctcga acctgtgacc ctctgattaa aagtcagatg ctctaccaac tgagctaacg
      901 gctcatatgg aggttaacgg gatcgaaccg ctgaccctct gcttgtaagg cagatgctct
      961 cccagctgag ctaaacctcc atggataact t
//
LOCUS       DAMEET010000213          933 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.227, whole genome shotgun sequence.
ACCESSION   DAMEET010000213 DAMEET010000000
VERSION     DAMEET010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.227"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..355
                     /locus_tag="PB780_002702"
     CDS             <1..355
                     /locus_tag="PB780_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734672.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..933
                     /locus_tag="PB780_002703"
                     /pseudo
     CDS             615..933
                     /locus_tag="PB780_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          357 a          110 c          196 g          270 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa gggttctgtt gcaaagtttt cca
//
LOCUS       DAMEET010000214          894 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.228, whole genome shotgun sequence.
ACCESSION   DAMEET010000214 DAMEET010000000
VERSION     DAMEET010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.228"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            253..876
                     /locus_tag="PB780_002704"
     CDS             253..876
                     /locus_tag="PB780_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDE0734673.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          329 a          129 c          167 g          269 t
ORIGIN      
        1 aaactaatgt ccttattttt gtgttcacat ttctgttacc taagaaatac ttaaaggagg
       61 tgatgtagcc agcaacgact acaacttgaa cccgctatct acagtttatt tgttgtttct
      121 tttgggtata ctctaatggt acaaatccat atttgcttaa agcgttgatt tttcagcggt
      181 ttttctttat tgcaaagtgt accctaagac ctgtatacta atagtaaata gattggatag
      241 gaatgatggc aaatgtattt agcttattta agagtttctt ccgacgatca aagtttagct
      301 cggcaacata agctaattga aaattggtca gtcaaaaata atattgctga agacgattta
      361 aaaattttcg aggaaaaagt ctctggaaaa aatattgaaa acagaccaga gctgcaagag
      421 cttatttctt tcatacgtga acgagatacg attattattc ctagtcttga tcgattagca
      481 agaaatagta aagatacaaa agacttactt caattgtttc gtagtaaagg tgcttctatt
      541 gaaatattag acctaccgtc gtttaatggg gtaactgacc ctgcattaag agacttgcta
      601 acaaatttag tcatagaagt gtttagctat gttgctgaaa acgaacggaa aaaaatcaaa
      661 gagcgtcaaa aacaaggcat tgaaattgca aagaaaaatg gtcgttatac tggtaaaaag
      721 attcaatacc atgccaatgc aacaggaaaa aacaaattga tttacgatga aattgttgat
      781 ggtctgaaga aaaaagaaac tatttctaaa atagcaaaga acgcaggagt tacaagaaaa
      841 accgtttatc gagtaaaaag agatttagga atgtaatatg ttccagttag aaag
//
LOCUS       DAMEET010000215          891 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.229, whole genome shotgun sequence.
ACCESSION   DAMEET010000215 DAMEET010000000
VERSION     DAMEET010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.229"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            283..522
                     /locus_tag="PB780_002705"
     CDS             283..522
                     /locus_tag="PB780_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734674.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          299 a          165 c          132 g          295 t
ORIGIN      
        1 ctatcctcct agcaactatt taaataccca aaaaaattac ctctagataa tatcatacct
       61 acgttttcac ttataaattt tttcataaaa aaagcgttta gaggcataat cgttaataca
      121 gatggtaacc aatgtatctg gtttgaactg ggatatcacc ctctccatat atgtattact
      181 aagactaata gatactataa aagacttgat agttttgcat tgattttgcc taatactgag
      241 cataggtgta tgctaaacga tactataagg agtgtgtaca taatgaaaag aattctgttt
      301 atcagtttat caaatttatt gttacttact gcttgtggga cttctgcatc aaagaacata
      361 ccttcagata cgcaaatttc gaatagtatc gattcaacaa tttctacaaa tgagaaaaat
      421 tctccggtag aaacttccga tataaaagtt tctacctctg caaaaaacag ggtaaagaaa
      481 ctgtcagcac gtctccttta tttcaaacaa actctgaatt agcttcttca tcttcttcag
      541 caaataatga tgatagtttc gcgtttactc ctaactaaat gcaaccttta caaatgcttt
      601 acgataacaa gcctgagcta aatgatcctg atatcagttt tgcatttttt gaaatgattg
      661 gatcggatga tctttttaaa actactagtt tgtctatccg aaaataggac agttctggca
      721 gtataggttt ttatcgagtt tctccaccag gcctcgttac agagacagat tctctaggcg
      781 aaccttttga ataaatcaaa aatcatgaac aatcactgcc aactaaataa agtgccttca
      841 aaagcttggt cttctcgctt ttgaaggcac tttatttaaa tctttatgaa a
//
LOCUS       DAMEET010000216          863 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.230, whole genome shotgun sequence.
ACCESSION   DAMEET010000216 DAMEET010000000
VERSION     DAMEET010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.230"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            68..>863
                     /locus_tag="PB780_002706"
     CDS             68..>863
                     /locus_tag="PB780_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDE0734675.1"
                     /translation="MHKISVIVPAYNVENYLENCLESISNQTHSNIEIIVVDDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKKISSLEQLKEHTIAERKWEIFDREQAMKNLFINNKIGYSAN
                     NKLFNRNLFDNIRFPLGKLMEDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSF
                     NPRIFDSFEVHEDILVFIDKFYPELSINVRTRYVYEAIRMMMTMIKN"
BASE COUNT          333 a          106 c          159 g          265 t
ORIGIN      
        1 ctaaaattgt tactggtaat gatacatttg taaaaataaa aaagatgatt tttaggaagt
       61 gaagagagtg cataaaatta gtgttatagt acccgcatat aacgtagaaa actatttaga
      121 aaactgctta gaatctattt ccaatcagac acattcaaat attgaaataa tagtagtcga
      181 tgatggttcc ccggatcgaa gtggggaaat tgcagaagaa tataaaaaga aagatatgcg
      241 attaaaagtg atacatcaag tgaatggtgg tttaagtgcg gctaggaatt ctggtcttga
      301 ttctgcaaca ggagattttg tgtgctttat tgacagcgat gattggatag aaccagacta
      361 tcttgagtta ctatattttg gtacgcaaac ttatggagca gatatctcgg tgctaaaaat
      421 aaaaaagatt tcaagtttag aacaactaaa agaacataca attgctgaga gaaaatggga
      481 aatatttgat agagaacaag caatgaaaaa cttatttata aataataaaa ttggctattc
      541 tgcaaataat aaattattca atagaaattt atttgataat ataagattcc cactgggtaa
      601 attaatggaa gataaggcta ctacttattt gttgatcgat cattccgaaa aagttgctgt
      661 caattcatct gctaaatatc actattacca aagccctaaa agtatactga gaggaagctt
      721 caatcctaga atttttgatt cttttgaagt ccatgaagat atactagtat tcatagataa
      781 gttttatccg gaattatcaa ttaatgttag aactcgctat gtctatgaag caataaggat
      841 gatgatgaca atgattaaaa ata
//
LOCUS       DAMEET010000217          863 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.231, whole genome shotgun sequence.
ACCESSION   DAMEET010000217 DAMEET010000000
VERSION     DAMEET010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.231"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..861
                     /locus_tag="PB780_002707"
     CDS             <1..861
                     /locus_tag="PB780_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HDE0734676.1"
                     /translation="EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPD
                     KYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKR
                     YKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIIS
                     YSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMS
                     RKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLS
                     PVEYRKQSIK"
BASE COUNT          374 a          112 c          140 g          237 t
ORIGIN      
        1 gagcaagaag aaatcaaggt taaaacaatc cttcagctca aaaaagaatt taaattgaat
       61 ctattacttt caattgcaca attagcgaag tcaacttatt attactgggt aaaaaaatta
      121 gataagccag ataaatatag caagataaag caagaaatta cagcgattgt aaaagaatca
      181 agaaactctt atggttatcg tagagtcact ttagcgttaa agatgaaagg atatacaatc
      241 aatcataaaa cagttagaaa attaatgtcc caaatgggac ttacctgtca aatccgaata
      301 aagcgatata aatcttataa aggaacagta ggaaaaattg ccaagaatgt gttaaaacga
      361 aatttttcag tagatacacc caataaaaaa tgggtgacag acgtcactga attcaagata
      421 aaaggaagaa aaatctatct atctcctata cttgatttat ttaacggaga aataattagt
      481 tatagtatat caactagtcc aacatataaa ctgattgaag aaatgcttca acaagcaatt
      541 aaaaaaaagg gaactgaggg ttcactgatt ctacattcag atcaaggatg gcagtatcag
      601 atgccacaat atcaaaaaaa attaaaagag aataacatta ttcaaagcat gtctagaaaa
      661 ggaaattgct tagataattc tgtaatagaa aatttttttg gtgtgcttaa atcagagttt
      721 ttttatcgag aaaaattccg atcaattgag atatttcaaa gtaaattaaa tgagtatatt
      781 aggtggtata ataacaaaag gataaaacta aagttaaatg gactatctcc agtagaatat
      841 cggaaacagt ccattaaata gtt
//
LOCUS       DAMEET010000218          825 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.232, whole genome shotgun sequence.
ACCESSION   DAMEET010000218 DAMEET010000000
VERSION     DAMEET010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.232"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            68..>825
                     /locus_tag="PB780_002708"
     CDS             68..>825
                     /locus_tag="PB780_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HDE0734677.1"
                     /translation="MNPTLITKKELLKKLDISTGVLADLIRNGMPKEGEMFNLDKIIT
                     WRENWSKNILGELEVGRVYTNKEISEKFKCFKQGGMRRSHQTNTLVLFSDQTGSNVYK
                     DKWLNGILQYTGMGLKGDQVLDKNQNKVLANSKSNFVKIHLFETFKPKEHTYLGEVYL
                     AGQIYTVNEKDSSGNSRKVYKFPLALINQEQLIEDKDIYNQEENQTRHIRNLSDAKLE
                     EEARKVSNYNKKMAAKYVNEEEFCFSKTKIYKRTR"
BASE COUNT          340 a           86 c          150 g          249 t
ORIGIN      
        1 tgagtttttt tcgatttttc aagttatcta atgcaattat attgatatca ttaggagctg
       61 cattgttatg aatccaacat tgattactaa gaaagaatta ctaaaaaaat tagatatttc
      121 tactggtgta ttagctgatt taattagaaa tggcatgcca aaagagggag aaatgtttaa
      181 tcttgataag attattacat ggcgagaaaa ttggtctaaa aacatattag gtgagttaga
      241 agttggtaga gtatatacta acaaagaaat ttcagagaag tttaaatgtt tcaaacaggg
      301 tggaatgaga agatctcatc aaactaatac tcttgtactg tttagtgatc aaacaggaag
      361 taatgtttat aaagataagt ggttaaatgg tattttacaa tataccggta tggggctaaa
      421 aggggatcaa gtattggata agaatcaaaa taaagtttta gccaattcaa aaagtaattt
      481 tgtaaagatt catttatttg aaacatttaa accaaaagaa cacacgtatc tgggagaagt
      541 atatttagca ggacaaatat atactgttaa tgaaaaagat agttcgggca acagcagaaa
      601 agtatataaa tttccattag ctttaataaa tcaagagcaa ttgattgaag ataaagatat
      661 atataaccag gaagaaaatc aaactagaca tattcgtaat ttatctgatg ctaaattaga
      721 ggaagaagca agaaaagtta gtaattataa taaaaaaatg gctgcaaaat atgttaacga
      781 agaggagttt tgcttctcaa aaacaaaaat atataaacgt acccg
//
LOCUS       DAMEET010000219          804 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.233, whole genome shotgun sequence.
ACCESSION   DAMEET010000219 DAMEET010000000
VERSION     DAMEET010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.233"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..716)
                     /locus_tag="PB780_002709"
     CDS             complement(<1..716)
                     /locus_tag="PB780_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDE0734678.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLY"
BASE COUNT          274 a          134 c           96 g          300 t
ORIGIN      
        1 atataaagtt gtgatatttt tccagctaaa gatccaccat taagaaattc ttcaatggct
       61 gtttgtaaaa ctaagtctgc agactttcct ataggtgaat gaatatagtt agttatagca
      121 ataatttttg cgccattttt tttagctatt ttcaatgaat cataagtatc tttcgttttc
      181 ccagataaag aaaaaatgat tactaaatca ttaactgtta ataaagaagc gacttgggct
      241 tgaaaatgag ggtctagtac agcttttgct tgaactccta cgcgaagaaa catattttct
      301 aaatcaagac tagtatttcc actagatccc actccaaata tatatatgtt ttttgattga
      361 gtgatcattt taatggcttc attcaatttt tcttcgtcta aaagcctaat agtggaatag
      421 agagcttctg ttaaactatt agcaatttca tcatagtact ttacactagt cggatgttct
      481 tttttttgac taaagtcttc ctttgcaatt tcaattttta aatcagaaaa accagaaaaa
      541 ccaatttttt gacaaaagcg aataatagta gcatcgccaa cttttgtgtt tttcttaata
      601 tcactcatcg tactataaat aatagttttc ccagaattta aaataaaatc agcgactttt
      661 ttttcagact tcgttaaaaa tggataattt tctttaatta aatagatata actcatacaa
      721 actcctctac aatttcaact tttctccatt atactccaaa ataataaaaa aaactcctaa
      781 atactccatt tttatcttta cttt
//
LOCUS       DAMEET010000220          803 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.234, whole genome shotgun sequence.
ACCESSION   DAMEET010000220 DAMEET010000000
VERSION     DAMEET010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.234"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            69..770
                     /locus_tag="PB780_002710"
     CDS             69..770
                     /locus_tag="PB780_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDE0734679.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT          290 a          147 c          138 g          228 t
ORIGIN      
        1 taaaacttta caaattcttt aaaaaaagtg acaataaata tagtgaacaa taaggagagg
       61 actttttaat gcgtgttaat aaaatcgatt ttcacttttc aaaactcaga tatcaacctg
      121 attttgaaca tttctcggat gaagagttcc aacagatcaa ggaacatact gttcttcgtt
      181 cttataaaaa aggacagatc cttttcgacg atggtgatcc cagacaacgg ttgtatatct
      241 tgaccaaagg actcgttcgt ttagaacgtt atgatgaaag tgcaacttat tactactatg
      301 actatgtgaa tacaaataac ctttttccta tgtcaggaat gtttggagaa gagacctaca
      361 cttatacagc tcaagcgatg actgatattg aaacattcta ccttcctgtt tcctactatg
      421 aaaaattagc acgaaaaaat cccgaacagt tgatctatat gatacaaaaa atttctaaag
      481 tagttgaaat acatgaattg cgtatccaaa tcggattgac ctctagcgcc tttgaccggg
      541 taaaacaaaa tttattcatt ttgaaagaag aattgggcgt accaacaaaa gatggtcata
      601 tcaccattcc ttatccgatc accttaaaag aattagcagt gaatagcggc acgacacgtg
      661 aaactgctgg acaagtaatc aaacaactac gtgattccgg gctgttagat tatgacaaaa
      721 agcgttttac tttctgtaag acagctacta ctccagctat gagagaataa cgcgaacaaa
      781 aataagcagt gaccctgata aat
//
LOCUS       DAMEET010000221          798 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.235, whole genome shotgun sequence.
ACCESSION   DAMEET010000221 DAMEET010000000
VERSION     DAMEET010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.235"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..197)
                     /locus_tag="PB780_002711"
     CDS             complement(<1..197)
                     /locus_tag="PB780_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDE0734680.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQ"
     gene            complement(194..>798)
                     /locus_tag="PB780_002712"
     CDS             complement(194..>798)
                     /locus_tag="PB780_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDE0734681.1"
                     /translation="LIINKNRKGNIFFKVAFFAPVVMSLVVISILWLYLLNPNEGLVN
                     TLFAKIGIASQPFLTSPKQAMYTLVFVSAWQGAGYQMLLFLGGMQNIPQDVYEAAEID
                     GFSKFQQFRYITMPLLKPTAIFILLTTLISAFKLIIQPMVMTQGGPMNSTMTMVYYIY
                     QTGFTDRMVGYSSSIALIFTTMIGLITIAQRKLVKEDDPA"
BASE COUNT          275 a          150 c          148 g          225 t
ORIGIN      
        1 tcttgataag ttttaaacca attagctgga ttcaaactag gtaaaaaagc tttgaaggaa
       61 gctaaatttt gatagacatc tgcttcaggc ttcatagagg aaactaccat ccacagcatt
      121 gggaaaatga ataatgccgc taaaaggaac agcaaacaat attctacgat cattagcggt
      181 ccaaatttct tggtcatgct ggatcatcct cctttaccaa ttttctttga gcaattgtaa
      241 ttaaaccaat catggtggta aagattaacg caatggagct ggagtagcct accatacggt
      301 ctgtaaatcc agtttggtaa atgtaataaa ccattgtcat agtagaattc attggtccac
      361 cttgagtcat taccattggc tgaatgatta gtttaaaggc cgaaatcaat gtggtaagta
      421 aaataaaaat cgccgtgggc tttaataatg gcatggtgat atagcgaaat tgctgaaact
      481 tagaaaaacc atcaatttcg gctgcctcat aaacgtcttg cggaatattt tgcattcccc
      541 ctaaaaacag cagcatctga tatcctgcac cttgccatgc cgatacgaaa acaagcgtat
      601 acattgcttg tttgggacta gtcaaaaaag gctgcgaagc aattccaatc tttgcaaata
      661 gcgtattaac cagaccttca ttaggattta acaagtacaa ccataaaatc gaaataacga
      721 ctaaggacat cacgactggc gcaaaaaagg ctactttaaa aaagatattt cctttacgat
      781 ttttgttgat aataagtg
//
LOCUS       DAMEET010000222          794 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.236, whole genome shotgun sequence.
ACCESSION   DAMEET010000222 DAMEET010000000
VERSION     DAMEET010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.236"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(560..>794)
                     /locus_tag="PB780_002713"
     CDS             complement(560..>794)
                     /locus_tag="PB780_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDE0734682.1"
                     /translation="LFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYEKG
                     WKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT          260 a          152 c           80 g          302 t
ORIGIN      
        1 taacctttgt gatttttctt gtttgaagaa tgttagaaga aaaatctcta aatacttata
       61 attattttat aagcaaaaaa atgctatcgg tagtatcacc gatagctgag gaaagtcaca
      121 gtagggacta tttgattaaa aaatatgttt attcaatcca tctatttatt tttgccttat
      181 taacatattg taataatgta agcaacaatg tattttaagt gtaatgcttt tatgcaccta
      241 tttaatttat cattctcatc tgatacaatc tcactcataa caatatgaat ttttcttctc
      301 attagtctca gttcaccatg aattatacat aacacccaat acaattattt ttccatactt
      361 ttttaagagg acaattacat cgtatatatc gctaagaatt gcaaccattc catttttaaa
      421 gacatatttc ctctaaaaaa ctaaaattct atctatccac gactaataat cccccacatt
      481 aaacccgtaa tcgaaccaga aaatttttct ttataactaa aacaaccatc attttcgagt
      541 tatcttaaac acatatttcc tattttttca tttctatcca tgcatgataa tattcagttc
      601 tgatattgtt ttcatccgta ttcgtcaata gatactggcc gatcacttct cctgttttcc
      661 atcctttttc atacattctt tctctgattt ctaaaatatt attacgttcc gcatggttca
      721 tctcggattt caataagaat cgacgataaa cagccgtatc ttttttctcc caaatcacgt
      781 cttgagtaaa taaa
//
LOCUS       DAMEET010000223          790 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.237, whole genome shotgun sequence.
ACCESSION   DAMEET010000223 DAMEET010000000
VERSION     DAMEET010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.237"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..781
                     /locus_tag="PB780_002714"
     CDS             <1..781
                     /locus_tag="PB780_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDE0734683.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          339 a          107 c          102 g          242 t
ORIGIN      
        1 agatatgcta gattactact attttgaaaa aaatttatca tataattatg taatacattt
       61 aaaaactttt acaggaatga tttttaaatt cgccaaccgt cgatatggat taaaatataa
      121 tcccgtaaat ggggtttctt tagcaagaaa acctaagaca aacgaagaaa taaaaaaaga
      181 aaaagatggt tatttatcaa aagaagaagt tcaaatgatt gctagtaaac aaaaaagttc
      241 acatcaacag tcacgttatt ctttaattac taaatttttg tttttaactg gactacgtta
      301 tggtgaattg atatctttga ctgaatctga ttatgatggt aataaaatta cggtttctgg
      361 tacttatgat tacgagttaa aaattaaaac aacaactaaa aatacaggtt cttatagaac
      421 cattgaatta tcaagtaatg ctaaagaaat catagatcaa ttaattgaag aaaacaaatt
      481 aattaagaat aataaagata aatacatttt catctctaaa aatggtaacc cgatatctat
      541 tcaagcatat aatcaatctt taagagcagt atccaaagaa ttaaacatag ataaaaaagt
      601 ttcttctcac atgttaaggc attcccacat ttctctactt acagaattag gaatcccatt
      661 aaaagccatt atggatcgtg taggacatga agattcaaaa acaacattaa agatttatac
      721 tcatacaact aaaaatatgc aaaatcaact agtagaaaaa ctaggaaaaa tagaaattta
      781 aattgcccct
//
LOCUS       DAMEET010000224          775 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.238, whole genome shotgun sequence.
ACCESSION   DAMEET010000224 DAMEET010000000
VERSION     DAMEET010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.238"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..660
                     /locus_tag="PB780_002715"
     CDS             <1..660
                     /locus_tag="PB780_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDE0734684.1"
                     /translation="YISLLCKEEVRELPKSNSAVGIDLGIIDFAILSTGQKFDNNKFT
                     SKMEKKLKREQRKLSRRALLAKQKGINLFEARNYQTQKRKVARLHEKVMNQRTDFLNK
                     LSTEIIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYKADWYGRKIIKV
                     DKWFPSSQICSNCGHKDGKKSLEIREWTCPICHTHHDRDINASINILTEGLRLHSMGL
                     A"
BASE COUNT          276 a          137 c          142 g          220 t
ORIGIN      
        1 tatatttctt tgttatgcaa agaagaagtt cgcgaactac ctaaatctaa ttctgctgtt
       61 ggtattgacc taggtattat agactttgca attctttcta ccggtcaaaa atttgataat
      121 aataaattta cgtccaaaat ggaaaagaaa ctcaaacgtg aacaacgtaa gttgtcaaga
      181 cgtgccttat tagctaaaca aaaaggtatc aatctgtttg aagctagaaa ttatcaaaca
      241 caaaaacgta aagtagcacg attgcatgaa aaagtaatga atcaacgtac tgattttctg
      301 aataagttga gtacagaaat tatcaaaaat cacgatatca tctgtattga agacttaaac
      361 acaaaaggca tgttgcgtaa tcataaacta gcaaaaagta tttcggatgt ttcttggtct
      421 agttttgtgt ctaaattaca atacaaggct gactggtacg gtcggaaaat catcaaagta
      481 gataagtggt ttccatctag ccaaatctgt tcgaattgtg gacataaaga cggcaagaaa
      541 tctctcgaaa ttcgagaatg gacttgccct atttgtcata ctcatcatga ccgagatatc
      601 aacgctagta tcaatatcct gaccgaaggt ctaaggctac actctatggg attggcttag
      661 atcaaataaa caagaaccgt aggaactacg gggatagctt ggtaaataag aggcacctct
      721 gttagtaaag taatacgcta tcaagtatgc tctcttccca agaagctccc acttc
//
LOCUS       DAMEET010000225          770 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.239, whole genome shotgun sequence.
ACCESSION   DAMEET010000225 DAMEET010000000
VERSION     DAMEET010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.239"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(135..512)
                     /locus_tag="PB780_002716"
     CDS             complement(135..512)
                     /locus_tag="PB780_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734685.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
BASE COUNT          257 a          122 c          132 g          259 t
ORIGIN      
        1 aaccaaaaaa gaagcagatc cgttagcggt tctgcttctt ttagtttatt atattttctt
       61 atcaataata ggaacctgat tttaaaagcg gctacctctt taccgtgcct ctttacatac
      121 aaaaaataga ggaattaagc gtaaatggct tggctattat aactaaaatg ctggttatgg
      181 agatgttgca gcaagttttc gagaaaagcg tattccatct cttgtccatt gaataagatc
      241 tggatacgat aatgattttt gaaacgcttc tgttcatgaa tctgactgat tgaaagttcg
      301 cctttgtgtt ctgtctgata aagaaaagtc cttttacggc attcagcaca gcgcttggtt
      361 gctcggcgga tttttcggaa tgtataaaaa ctagtatctt tattgcataa ataaacgtct
      421 ttgatcgaca gcaaatcatt atatagacag gaacgttctt cagctgttaa ttctgataaa
      481 aaaagctgac cttgttttat tgcatcttcc atcggcaagt gcctcctcga acccattatc
      541 caaattttta gttaattcta atataccatc attaagtata aatgtaaacg cttgttgatg
      601 gtataagatt ttcgtttctg ataaaacacg aacattaaaa gaatctaatg ccttaaatgt
      661 acatattttt gttgatttat aaaaagatga ttgctaaaat ttaaagtgaa aaaacgaaca
      721 aaaaacgatg tatttgcttt taatgtaaat aaaaaggaga gacgtacatg
//
LOCUS       DAMEET010000226          749 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.240, whole genome shotgun sequence.
ACCESSION   DAMEET010000226 DAMEET010000000
VERSION     DAMEET010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.240"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            74..697
                     /locus_tag="PB780_002717"
     CDS             74..697
                     /locus_tag="PB780_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734686.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT          285 a          117 c          145 g          202 t
ORIGIN      
        1 aacttttttg tctttctatt tcattttttg tatataatag aatagtcaaa attaaatagg
       61 agtggaggaa agcatgagga aaaaaagacc gtttcttctc ttttggttat tcggtttttt
      121 cttactgctt attttatccg cagcattttc aggaaaaatg acagcagatc ttgctcagaa
      181 agaaacagaa aaaatcgtag caagcaaaga atcagtagat aatttcaaaa aagaattctc
      241 aacttattgg tgggcggagt ataaaataac tgccaattac gaaaaaggcg aattggacat
      301 ttatttgcca aaaggaaaag aagaaaatca atttattaat atcgatgacg tacttagtgc
      361 gatttctctc agacaatatc aatttaaaga caagcatttg accgttttgc ttttgactca
      421 tgatggcgac aaagtagccc gtttgaaaaa tggaaaagtg atcaccgctt attctgaaaa
      481 agtagacaaa aaagtggtcg ccgatcgttt agcggaatgg aaaaagacaa atgaacaagt
      541 caagaaaaca actgtctctt ctcaaaaaaa cgagtcgaca ggaacaagtc cttcagattc
      601 tggcagtgga caaacgataa cgggaagtga agaaaatcaa gcctcaatta ttacgaatga
      661 gtcatcacaa ggacaatatc aatcctatgg acaatgaaaa acagaggttg tgaaatcagc
      721 gttttgacct gagtattaga gaaacaaac
//
LOCUS       DAMEET010000227          738 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.241, whole genome shotgun sequence.
ACCESSION   DAMEET010000227 DAMEET010000000
VERSION     DAMEET010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.241"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>738)
                     /locus_tag="PB780_002718"
     CDS             complement(<1..>738)
                     /locus_tag="PB780_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HDE0734687.1"
                     /translation="HTSCDDNGFILSTIITPGNIHDSQVAFQLVKQSKRLFPEINCVV
                     ADAGYKTPKFVHFLTHLNLRPCLPYSRPKGKKGLLSKNEFLYDEYFDCYICPQDQMLA
                     FSTVTREGYREYKSNPKECVNCPLLNQCTKSKNHQRVITRHVWGDLMDEVEHLRLTDL
                     NKSIYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWK
                     GKEPLFFCSKIRNEVDKKLFQARVTSL"
BASE COUNT          205 a          153 c          138 g          242 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat gactcgttga tgatttttac ttttggtaca ttggtttagc
      361 agaggacagt tcacacattc ttttggattg gatttgtatt ctcggtaccc ttctcgagta
      421 accgttgaaa aagcgagcat ttgatcttgc ggacaaatat aacaatcaaa atactcgtca
      481 tagagaaact cattttttga taatagcccc tttttccctt tgggccttga ataaggcaaa
      541 caaggtcgaa ggttcaaatg agtcaaaaag tgaacaaact taggtgtctt atacccagca
      601 tcagcgacaa cacaattgat ttcaggaaaa agtctcttcg attgtttgac gagctgaaag
      661 gcgacttgac tgtcatgaat atttccagga gtgatgattg tgctaagaat aaatccattg
      721 tcgtcgcatg aagtatgg
//
LOCUS       DAMEET010000228          711 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.242, whole genome shotgun sequence.
ACCESSION   DAMEET010000228 DAMEET010000000
VERSION     DAMEET010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.242"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..602
                     /locus_tag="PB780_002719"
     CDS             <1..602
                     /locus_tag="PB780_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDE0734688.1"
                     /translation="TMKKEFPIPISWTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGV
                     GGGALQEGRFIEGFSHPEMGHMLVVPHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGK
                     KGQEVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKL
                     VNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          200 a          140 c          181 g          190 t
ORIGIN      
        1 gtaccatgaa aaaagaattt ccgatcccga tttcttggac aacagatgtc aatgcggctg
       61 cctatggtga gtatgtcttc ggcagtggca aaggattgag cagtgtcgtt tactatacga
      121 tcggtacggg tgttggcggc ggtgccttac aagaagggcg ctttatcgaa gggtttagtc
      181 atccagaaat ggggcatatg ctcgtcgtcc ctcatccaaa agatgacttt gctggcagct
      241 gtccatttca tggcaactgt ttagaaggga tggccgctgg acccgccatc gaaaaacgct
      301 tagggaaaaa aggacaagag gttgcagaag atgatcctta ttgggaaatc gaagcctcct
      361 atattgctca atgtgcctac aatacaacct tgatgttttc tccagatgtg atcatttttg
      421 gcggtggtgt gatgaaacaa cgccatatgc tgcaaaacgt ccacgacgca tttgcaaaat
      481 tggtcaatgg gtatgtcgaa acacctgagt taagcaaata tatcgtgacc ccagcattag
      541 aagacaacgc tggaacgctc ggttgtttgg cattggctcg agaagcggtc ttacattcat
      601 aaacgaaaaa aggctcttcg acatttagtg ttggtagaga aagatacaac tgaaaattta
      661 gtcataacca gttaggcaga gtgcttaact ggtttttgtt ttacttggaa g
//
LOCUS       DAMEET010000229          689 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.243, whole genome shotgun sequence.
ACCESSION   DAMEET010000229 DAMEET010000000
VERSION     DAMEET010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.243"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            82..>689
                     /locus_tag="PB780_002720"
     CDS             82..>689
                     /locus_tag="PB780_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HDE0734689.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRK"
BASE COUNT          230 a          119 c          135 g          205 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaaatt gtcaggtatc tttgaccacc gttattcgta cgttgaaaga gtttaaaagc
      541 tacttaccaa agcaatctaa gaggattctc cccagagtat tgatggttga tgaatttcgt
      601 tcgcacgctt ccatagaaga taaaatgagc tttatttgcg cagatggcga aacaggacaa
      661 ttaattgatg tcttgcctac gcgtaaatt
//
LOCUS       DAMEET010000230          670 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.244, whole genome shotgun sequence.
ACCESSION   DAMEET010000230 DAMEET010000000
VERSION     DAMEET010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.244"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            154..417
                     /locus_tag="PB780_002721"
     CDS             154..417
                     /locus_tag="PB780_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDE0734690.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            417..>670
                     /locus_tag="PB780_002722"
     CDS             417..>670
                     /locus_tag="PB780_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDE0734691.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSART"
BASE COUNT          253 a           88 c          140 g          189 t
ORIGIN      
        1 ctctaggaaa aaattaacta gcaccacggg tataaaggaa acggataaca ctttacaatg
       61 ctgttttggt actccaattt aaacaaaaca atcgaattta cgtacttaat taagtacgtt
      121 agaatggaga tataaattag agggggagta gatatggcaa aaacagtctt aaatcctagc
      181 tcagcacgga aaaattttta taagttgttg aaagatgtaa atgagaatca tacggaaatt
      241 gaaatcatta gtgagcgcag tgaaaataat gcggtgctta ttggtttgga tgattggaaa
      301 gcaatcaaag aaacgctatt acttgagcaa ataggcactt tgggtgttgt tcgtaagcga
      361 gaaaaagatg atagtggatt tactaatgtt gatgaaattg attgggataa cttataatga
      421 ataactattc tgtgatgatt aaaaactcgg ctaaagctga tttaaagaaa atcaaacagt
      481 ctaatttgaa acctcaattt gagaaggtta ttcagacttt aaaggaagat ccctatctcc
      541 ctacccaatc atttgagaag ttaaaaccta cacatgaagg taggtattct agaagattga
      601 atagacaaca ccgggttgtt tataaggtag atgaagatga acacgtggtt gaaatttatt
      661 cagcaaggac
//
LOCUS       DAMEET010000231          661 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.245, whole genome shotgun sequence.
ACCESSION   DAMEET010000231 DAMEET010000000
VERSION     DAMEET010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.245"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>661
                     /locus_tag="PB780_002723"
     CDS             <1..>661
                     /locus_tag="PB780_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDE0734692.1"
                     /translation="YKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPI
                     SCSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNE
                     FGHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLF
                     KSITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFN
                     SV"
BASE COUNT          221 a          112 c          135 g          193 t
ORIGIN      
        1 tataagaaaa acaaatcaaa ctgtggtaga cgtccgcttg ttttacccga ggaacaatca
       61 gaatatattc aaagaaaggt tgttcaagga tggacacccg atgtgattgt tggtcgtgca
      121 gcgtttccta ttagttgttc tgctcgtacc atttatcgta tgttcaaaaa ggggctattt
      181 gattcttctg acttaccgat gaaaggcaag cgtaaaccga atggacatca agaaagacgt
      241 ggaaaacaaa ctttccgccg ctctattcat gaacgtgaaa aggattatag ccaattctca
      301 aatgagtttg gtcaccttga aggtgacact atcgtaggtc tgaaacataa aagtgctgta
      361 attaccttag ttgaacgatt atcaaaagtt atcatcacat tgaaaccgtg tggtagacaa
      421 gcgattgata ttgaaaaaaa attaaatcaa tggtttgaat ctgtaccgaa aaacctattc
      481 aaatccatca cttttgattg tggaaaggaa ttttcaaatt ggaaacagat cagtaatgtc
      541 aatgatattg ccatttattt cgctgatcca ggaacgccgt ctcaaagagg cctaaacgag
      601 aattctaacg gattgttacg tagagatggt ttattgaaat ctatggattt caattcagta
      661 g
//
LOCUS       DAMEET010000232          639 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.246, whole genome shotgun sequence.
ACCESSION   DAMEET010000232 DAMEET010000000
VERSION     DAMEET010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.246"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..282
                     /locus_tag="PB780_002724"
     CDS             <1..282
                     /locus_tag="PB780_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDE0734693.1"
                     /translation="LIQDDLHHTHRLWLMNKINLYFGHSYVFAFYDYRTNITEVDLVI
                     SNYYIDTGKTPLLLMKNIPTERNWRLFEKTIYQLKKEKEVVKSAFCPEY"
BASE COUNT          218 a           89 c          119 g          213 t
ORIGIN      
        1 ttaatacaag atgatttaca ccatacgcat cggttatggt taatgaataa aattaatctg
       61 tattttgggc attcttatgt ttttgctttt tatgattaca ggacaaatat aactgaagta
      121 gatttggtta tttctaatta ctacatcgac actggaaaaa cgccattact gttaatgaaa
      181 aatataccta ccgaaagaaa ctggcgtttg ttcgagaaaa cgatttatca attgaaaaaa
      241 gaaaaagagg ttgtgaaatc agcgttttgt cctgagtatt agagaaacaa acggaaaaat
      301 agtttttcat attttgccgt ttttgagcta ataccgaggg acagctgatt gaacaccatt
      361 tattgagaga ttgtgaaaaa actgtcctgc atcaagtaat aagaacagcc aagtaaaaac
      421 agcttctcat gtttgttgaa tattgtgact tgtgaccgag gagttggcaa gagaacaccg
      481 tttattcgga aaaagggact gtgacactac atgtcacagt ccctttttga ttgaattatc
      541 tcatttgttt tctaattttt tctttatttg agaaaatagt tgaactggat aaaaagtact
      601 ggacgtgtca aaattatttt ggatcaaatt agatctatt
//
LOCUS       DAMEET010000233          628 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.247, whole genome shotgun sequence.
ACCESSION   DAMEET010000233 DAMEET010000000
VERSION     DAMEET010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.247"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(350..>628)
                     /locus_tag="PB780_002725"
     CDS             complement(350..>628)
                     /locus_tag="PB780_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDE0734694.1"
                     /translation="KRNPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLS
                     QGGKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
BASE COUNT          214 a          101 c           98 g          215 t
ORIGIN      
        1 atctcatgca tttcctgatg tgattttaga aaaatttact tgctaaaaaa ctgtgaaaat
       61 gtaggagcta gcatccttgt agtaaatgat cgagctcttt attatacatt aaaaaagttc
      121 agaaattttg atgaagtagc caactacttt ttcatgagta aagcaacact agaaaaagaa
      181 tcctccagta cttagaattt actggaggat gtacttttga agaagcctat aggttattta
      241 aaaaatatta ctacaggaag gaacgagaat tacttcgtta cttataatag aacttagtct
      301 tatatagaag gcatagctca cattagctta tttgtcctat aaaaataaat tacggtattt
      361 tagaaaagct attttctctt ttttcaacga tcttcagcaa tttttcttta tcgtcaggta
      421 actcaagttc atgaattcta gcatgacaat tgggacataa cgcgatacag ttttcaatca
      481 catcttttcc accttgcgac aaatattcta tatgatgaca atgtaaataa ggtacaccgt
      541 cttttataaa tggccctttt tcttggcaaa gttgacaaat accttttgca agtcgtttaa
      601 catattcaga tacgaaggga ttacgttt
//
LOCUS       DAMEET010000234          593 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.248, whole genome shotgun sequence.
ACCESSION   DAMEET010000234 DAMEET010000000
VERSION     DAMEET010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.248"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            28..>593
                     /locus_tag="PB780_002726"
     CDS             28..>593
                     /locus_tag="PB780_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HDE0734695.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTT"
BASE COUNT          211 a          101 c          117 g          164 t
ORIGIN      
        1 tagaaaaaga tagcaggagg aatgacgatg aatcatttta aaggaaagca atttcagcag
       61 gatgtgatta ttgtagccgt gggctactat cttcgttata accttagcta tcgtgaagtt
      121 caagaaatct tatatgatcg tggcattaac gtttctcata cgacgattta tcgttgggtg
      181 caagaatatg gcaaactact ctatcaaatt tggaaaaaga aaaataaaaa atccttttat
      241 tcatggaaaa tggatgaaac gtacatcaaa attaaaggaa aatggcatta tttgtatcga
      301 gccatcgatg cagatggttt aaccttggat atttggttac gtaaaaaacg ggacacacaa
      361 gcagcctatg cttttcttaa gcggttagtg aagcagtttg atgaaccgaa ggttgtagtc
      421 acagataaag ccccctctat tacaagtgcc tttaagaaac taaaagaata cggcttttat
      481 caagggacag aacatcgtac cattaaatac ctgaataatt tgattgaaca agaccatcgt
      541 ccagtaaaga gacgcaataa attctatcga agtttacgca ctgcctctac cac
//
LOCUS       DAMEET010000235          573 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.249, whole genome shotgun sequence.
ACCESSION   DAMEET010000235 DAMEET010000000
VERSION     DAMEET010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.249"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(44..>573)
                     /locus_tag="PB780_002727"
     CDS             complement(44..>573)
                     /locus_tag="PB780_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HDE0734696.1"
                     /translation="PLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDAS
                     LVKEAFGRIPYSLTDVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVE
                     STYKSVKVEFVHQYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKR
                     ILDNERGSDTSGEAA"
BASE COUNT          167 a          138 c          100 g          168 t
ORIGIN      
        1 atcctcggat ctgattttct ccggcagaag cacaatcatt caattacgct gcctctccag
       61 aggtatcaga tccgcgctca ttatcaagga ttcgcttcgc caatctctgt tgtcgaattt
      121 ggatcggtgt ttcatacgcc aacgtgccat gtaagcgtag atagttccac caatgcacat
      181 aatcaaacaa ttctagacgt agctgtgcca gtgtctcaaa ttggtattga tgcacgaatt
      241 ctactttgac agatttatag gttgattcca caacggcatt atcatacgga caacccttcc
      301 tactcaatga acgagtaatt ccaaaaccat tcagaatctc atgaatggtt tggttatcaa
      361 attcctttcc ccggtctgta tgaaaaagct tgacgtctgt taaagaatac ggtatccgtc
      421 caaaggcttc ttttaccaat gaggcatctt tcttctcacc acaggaataa ccaataattt
      481 ctcgattaaa caaatcaagt attaagcaga tataatgcca ctttttcccc acgcgaacat
      541 aagtaagatc cgtaacgatg gcttccaatg gct
//
LOCUS       DAMEET010000236          555 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.250, whole genome shotgun sequence.
ACCESSION   DAMEET010000236 DAMEET010000000
VERSION     DAMEET010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.250"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            73..>555
                     /locus_tag="PB780_002728"
     CDS             73..>555
                     /locus_tag="PB780_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HDE0734697.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     F"
BASE COUNT          208 a          102 c           90 g          155 t
ORIGIN      
        1 catacaaaaa aggaagtccc ttctgtagaa taaagttaac gacaaccaat tcacagaaaa
       61 gaggacttcc ctatgaatga ttttactaca gaaattgtgc aaactctagt cactaaaggc
      121 gatttaaatg aattattccg ttcgcactta gaaaaagcga taaacacact cctacggact
      181 gaattaacgg cttttttaga ttacgaaaaa tatgatcgca ctggttttaa ttcaggtaat
      241 tcgagaaacg gttcttactt tcgatcaatc aaaaccgaat atggtgaatt aacattggaa
      301 atacctagag atcgtaatgg tgagtttaaa caacaaactt taccagccta caaaagaaca
      361 aacgatacat tggaaaccac tattatccat ttattcgaaa aaggtgttac gatgtctgaa
      421 attgctgatt tgatcgaaaa aatgtacggt catcactata ctccacaaac catgtccaac
      481 atgactaaag ttctgactga agaagtaaat gcctttaaat ccagagcctt aaatgataag
      541 tatgtcgcta ttttt
//
LOCUS       DAMEET010000237          539 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.251, whole genome shotgun sequence.
ACCESSION   DAMEET010000237 DAMEET010000000
VERSION     DAMEET010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.251"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>539)
                     /locus_tag="PB780_002729"
     CDS             complement(<1..>539)
                     /locus_tag="PB780_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HDE0734698.1"
                     /translation="WGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAIEKDGKLYL
                     IYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDFRDPKVFQR
                     KEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECPDLFELDGK
                     EVLILSPIQMTPQGNAYQNT"
BASE COUNT          145 a          126 c          105 g          163 t
ORIGIN      
        1 gtattttggt aagcattgcc ttgtggagtc atttgaatag gagaaagaat caagacttct
       61 ttgccatcta gttcaaacaa gtctgggcat tcccatacaa tgccttgttc tggagttccc
      121 tccagcaata cggaataaaa ctcccaagtt agtaaatctt tggaatggaa catcacaatt
      181 tgtccacgag catctgcagt tttagtagcg actacagtaa aatagtcttc ttttcgctga
      241 aacactttgg gatcgcggaa atcttcggtc gttgcaacag aagctaagtg atcttctgcg
      301 atgattggat ttccggcata tttttcaaag tgaatgccat cttctgaaac tgccaaacac
      361 tgaacttgtc gaacttgccc ttccacaaca ttgtgtcctg tataaatcaa atacagcttg
      421 ccatcctttt ctatggcact gccggaaaaa cagccttcct tgtcatagct ttcacctggt
      481 gccagtgcta ctggcaaatg ctcccagtga attagatcct tgcttttagc atggcccca
//
LOCUS       DAMEET010000238          533 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.252, whole genome shotgun sequence.
ACCESSION   DAMEET010000238 DAMEET010000000
VERSION     DAMEET010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.252"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..354
                     /locus_tag="PB780_002730"
     CDS             <1..354
                     /locus_tag="PB780_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDE0734699.1"
                     /translation="TQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVD
                     QEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISI
                     LKELEEHENWYAEVE"
     gene            355..>533
                     /locus_tag="PB780_002731"
     CDS             355..>533
                     /locus_tag="PB780_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734700.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRG"
BASE COUNT          211 a           75 c          119 g          128 t
ORIGIN      
        1 acccaagtaa aatcgatgag aaatgcgatg caatatcttg agtatatgga cttttttgtg
       61 aaagaatttg atacaaattt atatgttgct ggtgttattc ctttcatgtt ggaaagcggt
      121 gacgcagtag atcaggaaat gtataaacaa gcccaagaga tttatgggga acatttgatt
      181 aaaaatgttg tactcaaaaa tgcgcgctta aaacgatacg acggatcagg tattacgatg
      241 gagaagacaa agaaaggtct cttaaaacaa tgggatcgca aaagccacga attatttatc
      301 agtattttga aagaattgga agaacacgag aattggtatg cggaggtgga gtaattggct
      361 gacatcaaag gacttgatat ttttgggaac aacaaaggac tcgccaaaat aaaaaaagaa
      421 gcagaagcaa agaaaaagca gcaagcaatt acaccatctg aaaataagca attagaagtg
      481 caccaagttc aagaaataaa aacttcctcc aatgttgaaa gacagagtag agg
//
LOCUS       DAMEET010000239          517 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.253, whole genome shotgun sequence.
ACCESSION   DAMEET010000239 DAMEET010000000
VERSION     DAMEET010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.253"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            57..>517
                     /locus_tag="PB780_002732"
     CDS             57..>517
                     /locus_tag="PB780_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDE0734701.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYS"
BASE COUNT          191 a           72 c          105 g          149 t
ORIGIN      
        1 ataaatatgt tataatagta tcaaatgaat attgctaaaa gaaaagaggt aggaacgtgg
       61 tagcgaagaa atatgtaatc ggtaatttta aaggtggcgt aggtaaaagt acgtgcgcgc
      121 aaatgttagg gtttgaatca gcagttaatg aaggaaaaag aaccttgata attgacttgg
      181 atatgcaagg aaatacttct gatgtgatga acttaacgca tatgaacttc actaacgaag
      241 aaggaggcgg agaaggtgaa ccgattgttt acgaaaatac tgtgactgac gtgttgataa
      301 acaatcttcc acctaaagag gctgtctata aagtaatcaa taacctctat atacttccag
      361 cagacatgtc ctttgaaatg tatgatgatt ggatcaaaga ccaatataat gattcattat
      421 cccaattcaa atatatgaaa gaaaagctag aaccattatt tgatgagttt gacgtcatct
      481 atttagatgt cccaccttct atttctgtat atagtaa
//
LOCUS       DAMEET010000240          516 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.254, whole genome shotgun sequence.
ACCESSION   DAMEET010000240 DAMEET010000000
VERSION     DAMEET010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.254"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>516
                     /locus_tag="PB780_002733"
     CDS             <1..>516
                     /locus_tag="PB780_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDE0734702.1"
                     /translation="KHMNQAFNELRIREMNELRKVGQKSQAEKLKKNWRFLLKNRANI
                     NHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHELVEAFRDKDPDLFF
                     SLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNGKIEAKNTHIKTMKRVSYGF
                     KSFENMRIRIF"
BASE COUNT          183 a          102 c           97 g          134 t
ORIGIN      
        1 tcaaacacat gaatcaagcc tttaatgagt tgcgtatccg tgaaatgaat gaacttcgta
       61 aagtaggaca gaaaagtcag gcagaaaaac tgaaaaagaa ctggcgcttt ttgctaaaaa
      121 atcgtgcaaa catcaaccat tatgaataca aaacatggaa aagttttcga gcaccaaaat
      181 acccatttct tacggaagcg atgatgattg atcgattgct tggcttttct acgtccctga
      241 aggaggcgta tccctatttt catgaattag ttgaagcctt tcgagacaaa gaccctgact
      301 tatttttctc cttattggca gaacttcccg aaacgttaga tgacggcttt cgggaaaagc
      361 tccaaaacct tctgacctat gaagaaggca tcaccaacgc gatgatctat ccttacacca
      421 atggaaaaat agaagcgaag aatacccata taaagacaat gaaacgagta tcctacggat
      481 ttaaatcatt tgagaacatg agaattagaa tctttt
//
LOCUS       DAMEET010000241          512 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.255, whole genome shotgun sequence.
ACCESSION   DAMEET010000241 DAMEET010000000
VERSION     DAMEET010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.255"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            15..286
                     /locus_tag="PB780_002734"
                     /pseudo
     CDS             15..286
                     /locus_tag="PB780_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751573.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
BASE COUNT          190 a           78 c           91 g          153 t
ORIGIN      
        1 actagcacca cgggttaaat ctatattttg atatacaaat tacagcttca gatactaagt
       61 tgcataaacc ttttccggac cccatacaaa aggcaattga tgaattgggc ataaaaaata
      121 agaaacgatt tatattggtg attcattata tgacatgcaa agtgcgaaag cttgtgggat
      181 agcatttggt ttagctaaat ggggagccaa aaatatagaa aattttcaaa gtgtagatat
      241 aattattgat tctcctcaag aaatacttga tttgttagaa aaatagacaa agagcgtgag
      301 ataaggatag ggtctcacgc tctttgtttt tcgccattta aagattgaaa tttttgccat
      361 cgccgattgt aaaattaagc tagacaacta aaaaagtcat ttgtgctaca ctcaaaatag
      421 ttccgaccaa agaattataa ggagtgagta caaatgacct atacccatct tacaacggat
      481 gaacttgtaa taatagagtc ttatttcaaa at
//
LOCUS       DAMEET010000242          490 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.256, whole genome shotgun sequence.
ACCESSION   DAMEET010000242 DAMEET010000000
VERSION     DAMEET010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.256"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            149..388
                     /locus_tag="PB780_002735"
     CDS             149..388
                     /locus_tag="PB780_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002425012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HDE0734703.1"
                     /translation="MIVGNLGAQKEKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN"
     gene            437..>490
                     /locus_tag="PB780_002736"
     CDS             437..>490
                     /locus_tag="PB780_002736"
                     /inference="COORDINATES: protein motif:HMM:NF018061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HDE0734704.1"
                     /translation="MLVFQMRNVDKTSTVLKQ"
BASE COUNT          204 a           58 c           94 g          134 t
ORIGIN      
        1 tctttctcaa aataaacata aaagcccttt aaagagggct tttatatatt aatcacaaat
       61 cacttatcac aaatcactta tcacaaatca caagtgattt gtgattgttg atgataaaat
      121 aagaataaga agaaatagaa agaagtgagt gattgtggga aatttaggcg cacaaaaaga
      181 aaaacgaaat gatacaccaa tcagtgcaaa aaaagatata atgggagata agacggttcg
      241 tgttcgtgct gacttgcacc atatcataaa aatcgaaaca gcaaagaatg gcggaaacgt
      301 aaaagaagtt atggaaataa gacttagaag caaacttaag agtgtgttga tagtgcatta
      361 tcttaaaatt ttgtataata ggaattgaag ttaaattaga tgctaaaaat ttgtaattaa
      421 gaaggaggga ttcgtcatgt tggtattcca aatgcgtaat gtagataaaa catctactgt
      481 tttgaaacag
//
LOCUS       DAMEET010000243          473 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.257, whole genome shotgun sequence.
ACCESSION   DAMEET010000243 DAMEET010000000
VERSION     DAMEET010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.257"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..374
                     /locus_tag="PB780_002737"
     CDS             <1..374
                     /locus_tag="PB780_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDE0734705.1"
                     /translation="YLFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATV
                     GVLSFISSWNDYIWPLMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLII
                     VYIVAQKYILQGLGGSGTGIK"
BASE COUNT          155 a           82 c           85 g          151 t
ORIGIN      
        1 tctatctgtt tagaaatttc tttatcgcga ttccagaaga aatcattgaa tcagcgaaat
       61 tagatggtgc aaatattgtt aaaattttct ttcacatcat gctgccaatg tctaaaccag
      121 ccatagccac agtaggtgtc ttatccttta tctccagctg gaatgattat atttggccgt
      181 taatggtctt aactgacagc agcaaatttt caatgcaagt ggcaattact acgatcaata
      241 ctacacagcc agtttatatc aatcaagtga tggctgtttt aaccattgca acgattccac
      301 tgattattgt ttatatcgtt gcacaaaaat atattctgca gggacttggc ggttctggaa
      361 cgggtataaa ataggagtga atagtgaatg ttgcaaaaac ttttaaaggc caatgagttt
      421 attgagaaaa atgctcaaac agtcaatcaa gaataccgga ataaatttca ttt
//
LOCUS       DAMEET010000244          471 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.258, whole genome shotgun sequence.
ACCESSION   DAMEET010000244 DAMEET010000000
VERSION     DAMEET010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.258"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>471
                     /gene="scm"
                     /locus_tag="PB780_002738"
     CDS             <1..>471
                     /gene="scm"
                     /locus_tag="PB780_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HDE0734706.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVIL"
BASE COUNT          173 a          118 c           81 g           99 t
ORIGIN      
        1 acatcagctt caagtgacag tacagatact acctctacca caagtgatag cacggacacg
       61 tcagcttcaa gcgacagtac ggatacatct accacaacaa gtgatagcac ggacacgtca
      121 gcttcaagtg acagtacaga tactacctct accacaagtg atagctcaga ttcatctacc
      181 actacaagtg atagcacgga cacgtcagct tcaagtgaca gtacagatac atcctctacc
      241 acaagtgata gctcagattc atcaacttca agcgatagta cagacacgtc gtctaccaca
      301 agcgatacac gtcatgcaag tgtctccttc tcaaaaacaa gttcggataa caggacgaac
      361 tttaacttaa ctaataaaac aagcaatgac aatggaaaca aatcaaaaag agcacttcct
      421 aaaactggaa gtcaatcgaa taattggata acactcgcag gcgttatatt a
//
LOCUS       DAMEET010000245          468 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.259, whole genome shotgun sequence.
ACCESSION   DAMEET010000245 DAMEET010000000
VERSION     DAMEET010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.259"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>468
                     /locus_tag="PB780_002739"
     CDS             <1..>468
                     /locus_tag="PB780_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDE0734707.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          155 a           86 c           83 g          144 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DAMEET010000246          463 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.260, whole genome shotgun sequence.
ACCESSION   DAMEET010000246 DAMEET010000000
VERSION     DAMEET010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.260"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(204..>463)
                     /locus_tag="PB780_002740"
     CDS             complement(204..>463)
                     /locus_tag="PB780_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDE0734708.1"
                     /translation="KYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRR
                     REKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          165 a           59 c           80 g          159 t
ORIGIN      
        1 gaattaaaaa tattaaacag gaatttatcg aaaatggtag aaaagaaatt tataaaagga
       61 cttataaaga ttgattatat aatatatgaa aagctattat aaaagacatt agtattaaat
      121 agtttaaaaa aatgaaaaat aataaaggaa gtgagtcaat tccagactcc tgtgtaaaat
      181 gctatacaat gtttttacca tttctactta tcaaaattga tgtattttct tgaagaataa
      241 atccattcat catgtaggtc cataagaacg gctccaatta agcgattggc tgatgtttga
      301 ttggggaaga tgcgaataat cttttctctt ctgcgtactt cttgattcag tcgttcaatt
      361 agattggtac tctttagtcg attgtgggaa tttccttgta cggtatattg aaaggcgtct
      421 tcgaatccat catccaatga tgcgcaagct tttgaatatt ttg
//
LOCUS       DAMEET010000247          460 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.261, whole genome shotgun sequence.
ACCESSION   DAMEET010000247 DAMEET010000000
VERSION     DAMEET010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.261"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>460)
                     /locus_tag="PB780_002741"
     CDS             complement(<1..>460)
                     /locus_tag="PB780_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDE0734709.1"
                     /translation="EGDTIVGIHHKSAVITLVERLSKAIIALKPEGRKAVDIENSINE
                     WLQSVPKNLFKSITFDCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRK
                     DGLPKEMEFNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCL"
BASE COUNT          124 a           81 c           93 g          162 t
ORIGIN      
        1 agacaaaact ttccatttac gtaactcaaa aaaacttcta atggtgtttg gtaatttagt
       61 gattttctag ggatatggtt tcttttggac gcaacggatg agatgaatcc ttgattgact
      121 tggttgaatt ccatttcttt tggtagtcca tcttttcgga ggagtccgtt tgaatgttca
      181 tttaatcccc gttgggaagg cgttcctggg tctgcgaaat aaatatcaat atcattcgta
      241 ttactaatac ttttccaatt agagaattct tttccacaat caaaggtaat tgatttgaaa
      301 agatttttgg gtacggattg aagccattca ttaatcgaat tttcaatatc aactgcctta
      361 cggccttctg gtttcaaggc gataatggct tttgaaagtc gctcaacgag tgtgatgacg
      421 gcacttttat ggtggatgcc gacaatcgta tcaccctcta
//
LOCUS       DAMEET010000248          459 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.262, whole genome shotgun sequence.
ACCESSION   DAMEET010000248 DAMEET010000000
VERSION     DAMEET010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.262"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            39..290
                     /locus_tag="PB780_002742"
     CDS             39..290
                     /locus_tag="PB780_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDE0734710.1"
                     /translation="MTVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHA
                     GMNYAVKLYKEGNMTVNQICEITNVSRASLYRKLSEVNN"
BASE COUNT          180 a           61 c           84 g          134 t
ORIGIN      
        1 ttgatttatc agaagataat ccatacagcc aattcttaat tactgtaatg gctggtgtta
       61 accaattaga gcgagatctt attcggatga gacaacgtga agggattgaa ttggctaaga
      121 aagaaggaaa gtttaaaggt cgattaaaga agtatcataa aaatcacgca ggaatgaatt
      181 atgcggtaaa gctatataaa gaaggaaata tgactgtaaa tcaaatttgt gaaattacta
      241 atgtatctag ggcttcatta tacaggaaat tatcagaagt gaataattag ccattctgta
      301 ttccgctaat gggcaatatt tttaaagaag aaaaggaaac tataaaatat taacagcctc
      361 ctagcgatgc cgaaaagccc tttgataaaa aaagaatcat catcttaaga aattcttagt
      421 catttattat gtaaatgctt ataaattcgg ccctataat
//
LOCUS       DAMEET010000249          456 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.263, whole genome shotgun sequence.
ACCESSION   DAMEET010000249 DAMEET010000000
VERSION     DAMEET010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.263"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..152)
                     /locus_tag="PB780_002743"
     CDS             complement(<1..152)
                     /locus_tag="PB780_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDE0734711.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYA"
     gene            complement(293..456)
                     /locus_tag="PB780_002744"
                     /pseudo
     CDS             complement(293..456)
                     /locus_tag="PB780_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236347.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          145 a          104 c           65 g          142 t
ORIGIN      
        1 gcatattcat caggctcata attaagctca ctaattgcag ccattacttt ttctttggta
       61 acctctttca cctttactcc atttattact ctagataccg tacccacacc aactcctgct
      121 aacgcagcaa catccttcat agtagttttc acttcacgaa caactcctct caaaagaatt
      181 tcaggtatta ttataccaaa agaaggaaac gattccaaat tagcaggaat atttttattt
      241 taaaatagat attagacgtg aagcttttcg taaaaaaatt ttcgtattca gatttaccgg
      301 attgactctg tcgccaatat aggccggcat actttgtcaa ttttatttca tcagaaaaac
      361 gttcaattgg accaatttca gcgagtattc cagcagccta aacaggacca atacctggga
      421 tactggttaa acattgatac tccggtagca ccacga
//
LOCUS       DAMEET010000250          454 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.264, whole genome shotgun sequence.
ACCESSION   DAMEET010000250 DAMEET010000000
VERSION     DAMEET010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.264"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            52..>454
                     /locus_tag="PB780_002745"
     CDS             52..>454
                     /locus_tag="PB780_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HDE0734712.1"
                     /translation="MGRAIQTIYNVVNKFKQGKTALDYWHQYKENKKKCGRKVIQLPA
                     HEVDYIKEKVTLGWTPDVIIGRKERPVSCGMRTLYRLFSKGIFDIDTLPMKGKRKPNG
                     HQEKRGKQQYQRSIHDRPDNYPDFNSEFGHLE"
BASE COUNT          164 a           79 c           89 g          122 t
ORIGIN      
        1 atagaatcat attatcttca acataataag ccggttgaaa tcgctaaccg aatgggacgt
       61 gctatacaaa ctatttataa tgtagtcaat aagttcaagc aaggcaagac tgctcttgat
      121 tattggcacc agtataaaga aaataagaaa aaatgtggta gaaaagtcat tcaattacct
      181 gctcatgaag tagattacat taaagagaaa gtcactcttg gttggacgcc tgacgtcatt
      241 atcgggcgaa aagaaaggcc tgtttcatgc ggtatgagaa cactttatcg tttattttct
      301 aaaggaatat ttgatattga cacactaccg atgaaaggta aaagaaaacc caatggccat
      361 caggaaaaac ggggaaaaca acagtatcag cgctcaatcc atgatcgacc tgataattat
      421 cctgatttca attctgagtt tggtcacctt gaag
//
LOCUS       DAMEET010000251          453 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.265, whole genome shotgun sequence.
ACCESSION   DAMEET010000251 DAMEET010000000
VERSION     DAMEET010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.265"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            17..240
                     /locus_tag="PB780_002746"
                     /pseudo
     CDS             17..240
                     /locus_tag="PB780_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010624568.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
BASE COUNT          160 a           65 c           96 g          132 t
ORIGIN      
        1 aggcaattat agggctatga ttacggttcg tgtaagtgac gccaaaaaag aatggctttc
       61 ttatatggct gaattttatg ggattacttt atctgaactt ctaaaaaact attcgatgga
      121 acaactagaa gatgaataca atcgtcaaac ggctgaaatt gcccataaac attggataag
      181 aagtgggaaa acaacagtat tcatggaaga atccttaatg aatttggtgg gctggaatga
      241 cctgccgttt aaaattcacg cccgaaattc agaagcagct ctgattggca aatacgtgta
      301 ttggtagaga tagaagataa tcaattcgtt gttaccgcta ttcaagtagg gcaccgaaga
      361 aatgtctatg attaagagac tgtttatttt tacaaaaaaa taataactgg aatctagtta
      421 aaactcttga agtgagagat ttgactagat ttt
//
LOCUS       DAMEET010000252          440 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.266, whole genome shotgun sequence.
ACCESSION   DAMEET010000252 DAMEET010000000
VERSION     DAMEET010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.266"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            11..>440
                     /locus_tag="PB780_002747"
     CDS             11..>440
                     /locus_tag="PB780_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDE0734713.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          168 a           57 c           88 g          127 t
ORIGIN      
        1 ggtgcttttt ttgcggaaat atacatttgt ttttaagaaa aaagtagttt cagactattt
       61 aaataacgaa ggcggctaca aatatcttgc acataaatat caaataaatc gtacactggt
      121 tagacattgg gtaaggattt ataactatca tggttgggaa ggcttggttg gaggtggcaa
      181 aagctacact acaaaattta aacttgatgt tatagaatat atggaaacaa atggtctttc
      241 tatccaagaa actgctaaaa aatttaatat cggttcaaat agaactctaa gtaaatggat
      301 agagcaatat gaagaaggcg gtgcttcttc acttgagagc caaaaaaggg gcagaaaaat
      361 tagtatgaat tccaagctaa acattcctaa aaaacttaaa gatgagtctc ttgaagaaga
      421 agttattcgt ttaagagcag
//
LOCUS       DAMEET010000253          434 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.267, whole genome shotgun sequence.
ACCESSION   DAMEET010000253 DAMEET010000000
VERSION     DAMEET010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.267"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>434
                     /locus_tag="PB780_002748"
     CDS             <1..>434
                     /locus_tag="PB780_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDE0734714.1"
                     /translation="LPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAEKEKEYIQ
                     MVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQIAAQELL
                     TRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLD"
BASE COUNT          143 a           71 c           86 g          134 t
ORIGIN      
        1 ttacctacga tttggcatcc cttcttttcc gagtttgcct actatgtaga ggaagctctc
       61 agccaaaaaa attataagct ttttttgtgt aatgcgaacg gcagtgcgga aaaagaaaaa
      121 gaatatatcc aaatggtcaa gcagagtaaa gtagacggaa ttataggtat tacctattca
      181 gacattgata aatatgtatc atcaaattta ccatttgtca gtatagaccg ccatttttcg
      241 gaacaggttt cttatgtaac ggcagataat tatttaggtg ggcaaattgc cgctcaagaa
      301 ctacttactc gtaactgtaa aaacttgtgc tatgttgggg gtgtttcacc ttaccctaac
      361 gaaacaaaca atcgtaaagc tggtttttac aatatttgtg aggaaaataa aatagctgtg
      421 aaggtattgg atat
//
LOCUS       DAMEET010000254          421 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.268, whole genome shotgun sequence.
ACCESSION   DAMEET010000254 DAMEET010000000
VERSION     DAMEET010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.268"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(170..355)
                     /locus_tag="PB780_002749"
     CDS             complement(170..355)
                     /locus_tag="PB780_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
                     /protein_id="HDE0734715.1"
                     /translation="MQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRA
                     WRKMLDDELIPLENELR"
BASE COUNT          120 a           85 c           62 g          154 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagaggtt
      361 ctgttgcgaa gtttgaaaat caaacgcgcc ctctctgaac tccaaatatc ttaggctgtt
      421 a
//
LOCUS       DAMEET010000255          420 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.269, whole genome shotgun sequence.
ACCESSION   DAMEET010000255 DAMEET010000000
VERSION     DAMEET010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.269"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            74..403
                     /locus_tag="PB780_002750"
     CDS             74..403
                     /locus_tag="PB780_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDE0734716.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          162 a           49 c           76 g          133 t
ORIGIN      
        1 cttgtaaata cattatctcg tggtataatg tatttactaa gtaattacat actgttagaa
       61 atgagggatt taaatgatta caaaaacaag aaaacaaggc aattcaatca tgttgacagt
      121 acctaaagat tttaatgttc ctaatggagt cgaagttgaa gcaaagttgg tagagaatgg
      181 tattctttat gaatttgttg aaccacaaaa agaattttat gattttagcg aagatatttt
      241 gtctgatata attgcagaag gctacgataa agatgagatt cttgtagaat ttaagaatag
      301 aaaaaacaaa atgcattctt cctttagaga tattgctgaa gatacactta ctaattctaa
      361 agtaatgact aaagaggagt tggcaacaga aattggctta tgaaatcgtt ccttcaaaac
//
LOCUS       DAMEET010000256          403 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.270, whole genome shotgun sequence.
ACCESSION   DAMEET010000256 DAMEET010000000
VERSION     DAMEET010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.270"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..380
                     /locus_tag="PB780_002751"
     CDS             <1..380
                     /locus_tag="PB780_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDE0734717.1"
                     /translation="EICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQ
                     DLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQ
                     KFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          155 a           72 c           63 g          113 t
ORIGIN      
        1 aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa gaagcgaata
       61 ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg acgcagtcac
      121 tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt cgtagaacca
      181 tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac agccgtagaa
      241 aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt tttgatacat
      301 acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc gatacattag
      361 tttcaatgtt tactgagtaa ctaattattt tgcaggagga caa
//
LOCUS       DAMEET010000257          347 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.271, whole genome shotgun sequence.
ACCESSION   DAMEET010000257 DAMEET010000000
VERSION     DAMEET010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.271"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>347)
                     /locus_tag="PB780_002752"
     CDS             complement(<1..>347)
                     /locus_tag="PB780_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDE0734718.1"
                     /translation="QFSDETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYE
                     DKVNAAITTNTLPDVITLDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYD
                     GKMYAFGFSESSV"
BASE COUNT          101 a           86 c           55 g          105 t
ORIGIN      
        1 cacactggac tctgagaaac caaacgcata cattttgcca tcgtaggttc cttgctgaat
       61 aatgctgtct aatacatcat ccatgtccgc atctttcaaa taatcatcta atggagcaat
      121 gactcctgaa tgagcataag ctgcagtatt cggtccatct aacgtaataa catcaggaag
      181 ggtattggtt gtaattgcag catttacttt atcctcatat ccaccgccgc ttccgctacg
      241 aggaatatac tcagtaacag ctttatattc accctcgtat tcatcattaa aattgttcac
      301 aatctctgcc caagcttttc cttcaggcgt ttcatcagaa aactgaa
//
LOCUS       DAMEET010000258          332 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.272, whole genome shotgun sequence.
ACCESSION   DAMEET010000258 DAMEET010000000
VERSION     DAMEET010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.272"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>332
                     /locus_tag="PB780_002753"
     CDS             <1..>332
                     /locus_tag="PB780_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734719.1"
                     /translation="IILSFLGKVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAE
                     YTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQ
                     GLYGWNVT"
BASE COUNT          103 a           53 c           78 g           98 t
ORIGIN      
        1 attatacttt ctttcttagg gaaagtacaa atggaagtga ttagtgcact gttgcaagaa
       61 aagtatcatg tggagataga actaaaagag cctacagtca tttatatgga gagaccgtta
      121 aaaaatgcag aatataccat tcacatcgaa gtgccgccaa atcctttctg ggcttccatt
      181 ggtttatctg tatcaccgct tccgttggga agtggaatgc agtatgagag ctcggtttct
      241 cttggatact taaatcaatc atttcaaaat gcagttatgg aagggatacg ctatggttgc
      301 gaacaaggat tatatggttg gaatgtgacg ga
//
LOCUS       DAMEET010000259          324 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.273, whole genome shotgun sequence.
ACCESSION   DAMEET010000259 DAMEET010000000
VERSION     DAMEET010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.273"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            71..>324
                     /locus_tag="PB780_002754"
     CDS             71..>324
                     /locus_tag="PB780_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDE0734720.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDT"
BASE COUNT           95 a           57 c           62 g          110 t
ORIGIN      
        1 tttatggatg atgtctacag tcttttcttt tataatagat ttatgaaaac gaatgcaagg
       61 agtgatagct atgatgtcta aaaacaatac gaatggtaga aatcaatttg cgatgcttac
      121 gattgatgat ctagttcctc aagatcatct cgttcgaaag attgatgccg cgcttgattt
      181 tgaatttatt tatccaatcg tcgaagccac ttactccgat ttaggtcgcc caagtatcga
      241 tccagtgatt ctgatcaaat tggtgtttat tcagtattta ttcggtatcc gttccatgcg
      301 acaaaccatt aaggaagtcg atac
//
LOCUS       DAMEET010000260          309 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.274, whole genome shotgun sequence.
ACCESSION   DAMEET010000260 DAMEET010000000
VERSION     DAMEET010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.274"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>309
                     /locus_tag="PB780_002755"
     CDS             <1..>309
                     /locus_tag="PB780_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734721.1"
                     /translation="GLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAP
                     QEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLT
                     E"
BASE COUNT           96 a           55 c           60 g           98 t
ORIGIN      
        1 ggcttatact atagccctgt tagtacccca gcagattttc ggatgcttgc tcctattgta
       61 ttggaacaag tcttaaaaaa agctggaaca gaattgttag agccatatct tagttttaaa
      121 atttatgcgc cacaggaata tctttcacga gcatacaacg atgctcctaa atattgtgcg
      181 aacatcgtag acactcaatt gaaaaataat gaggtcattc ttagtggaga aatccctgct
      241 cggtgtattc aagaatatcg tagtgattta actttcttta caaatggacg tagtgtttgt
      301 ttaacagag
//
LOCUS       DAMEET010000261          304 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.275, whole genome shotgun sequence.
ACCESSION   DAMEET010000261 DAMEET010000000
VERSION     DAMEET010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.275"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            22..>304
                     /locus_tag="PB780_002756"
     CDS             22..>304
                     /locus_tag="PB780_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDE0734722.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVII"
BASE COUNT          113 a           56 c           59 g           76 t
ORIGIN      
        1 atacaaggag agtgaacata aatgacttat acccatctta caccaaacga gcttgtaatg
       61 atagaagcat attttcatca agaaactccg gttgctatcg ttgcgaagca gcttaaacgt
      121 ggacgccaaa caatttacaa tgtctataac tttctcaaat gtggtggaac agcacttgaa
      181 tactttgaac aatacaaaga aaataagcga cgttgtggga gaaccgaaat catttttcct
      241 gctgaggaaa aagaatacat tgaaaaaaga tcaactgaag gttggacccc agacgtcatt
      301 attg
//
LOCUS       DAMEET010000262          291 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.276, whole genome shotgun sequence.
ACCESSION   DAMEET010000262 DAMEET010000000
VERSION     DAMEET010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.276"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT           81 a           43 c           41 g          126 t
ORIGIN      
        1 cctcatttta ggttatcatt tgtaatttta tttcacagtt gatatgattg ttatgtaaaa
       61 taaaaaagtt taggatacat cgaatttttg gggaaattct tttgggggta tcctaaatct
      121 tttctattat attcccatat tttctattat tggtctagac gtatgatggt aaactttgta
      181 cctatctgat tgctgaatca ttaattgctt atcattagcc atctttgttt caagttcgct
      241 agatgatcat tttattcaca tcctaacaaa cttgtcacat ttatagattc t
//
LOCUS       DAMEET010000263          291 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.277, whole genome shotgun sequence.
ACCESSION   DAMEET010000263 DAMEET010000000
VERSION     DAMEET010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.277"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          132 a           36 c           46 g           77 t
ORIGIN      
        1 ccttaggcag cttttgctgt gtagaaagga ataaataata acgaaagttc aaaagctaat
       61 gccgactatt ctagacatta aagaagacga aaacgaacga taaagaaaga aaagttatga
      121 ataatcgttt attgaaaaaa acttgaaaaa atacaaaaaa ttcgttgacc aagctaaatt
      181 tagttggtat attattactt gtccaaaaga cagaagaaaa tatttattca aaaacataac
      241 aaaaaagtta ttgacatgtt actaacgaca tgataagata actgagtcgt t
//
LOCUS       DAMEET010000264          291 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.denovo.278, whole genome shotgun sequence.
ACCESSION   DAMEET010000264 DAMEET010000000
VERSION     DAMEET010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.denovo.278"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(8..>291)
                     /locus_tag="PB780_002757"
     CDS             complement(8..>291)
                     /locus_tag="PB780_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GH32 C-terminal domain-containing protein"
                     /protein_id="HDE0734723.1"
                     /translation="DVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEI
                     FRDTSAIELFLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
BASE COUNT           89 a           68 c           45 g           89 t
ORIGIN      
        1 ctcccttcta tagattaatt aagccgcttt ccaatgaaaa ggttacttca ctgtctgcca
       61 gcaaaagaaa atcctgtcct ttttctgtct cataaaaagt tgtactcata gtcagacgat
      121 catttagaaa aagctcaata gctgaagtat cacggaaaat ttcgatggtt agacaattat
      181 tttccaaagg aatcgtcaca ctgcgctttt tcaacacttc cttttcagct cctgataaat
      241 catacggcat gtgttccctg gaagcagtaa agcactcccg ttcaacatca t
//
LOCUS       DAMEET010000265        22363 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.guided.044, whole genome shotgun sequence.
ACCESSION   DAMEET010000265 DAMEET010000000
VERSION     DAMEET010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.guided.044"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(116..2479)
                     /locus_tag="PB780_002758"
     CDS             complement(116..2479)
                     /locus_tag="PB780_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HDE0734724.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKLGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRNDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFHSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAATLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            2927..3148
                     /locus_tag="PB780_002759"
     CDS             2927..3148
                     /locus_tag="PB780_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDE0734725.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            3513..4580
                     /locus_tag="PB780_002760"
     CDS             3513..4580
                     /locus_tag="PB780_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009255061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDE0734726.1"
                     /translation="MCALKLGSLFDGIGVFPLAASRNGIIPVWASEIEKSPIAITKRH
                     FPGMEHLGDITKIHGGHIPPVHIITFGSPCQNLSKIGKREGLAGSKSGLFFQAIRIIE
                     EMRCATNGLYPVIAVWENVMGAFSSNDRMDFRAVLQSFSDTEISMPPTGGWANAGMVR
                     GGKTDLCWRLMDAQYWGKPRLVQRRKRIFLVADFRGQRSAEILFKPRDMFPIPPPCTD
                     YGLPAAGASRISLDKTRGKIPIIRPFQERRMRSAAKSGNQSAFLASFGRPDEPFPTLL
                     AKAVNMISFWYEGEEKDGVLRNLTPVECERLMGLPEGWTAYGNLGQPISDNARCKALG
                     NAIALPCADYIMAGIAETIHE"
     gene            4693..5007
                     /locus_tag="PB780_002761"
     CDS             4693..5007
                     /locus_tag="PB780_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDE0734727.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKTEQRRINGRMAILSRSFNLY
                     SDVQRADDIVVILPAEAGEKHFDFEERVKLVNPRITAEGYKIGTRGFTNYILHADDMV
                     KA"
     gene            5029..5406
                     /locus_tag="PB780_002762"
     CDS             5029..5406
                     /locus_tag="PB780_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDE0734728.1"
                     /translation="MRLANGIVIDKEATFGALKFSALRREVHLQNEDGSVSEEIKERT
                     YDLKSRGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFQGAEVDWYIKAE
                     DIVLKKGAAMNPQPPKKDAPPVK"
     gene            5445..6842
                     /locus_tag="PB780_002763"
     CDS             5445..6842
                     /locus_tag="PB780_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDE0734729.1"
                     /translation="MKQLFHRGKRIRPTDKDLVFHTALASLFPVFLLVVLLFHIRQIA
                     GTNWQEESLSQIVQDVNIPYLLFSMGVAGLVCLTAVLLFWRYRRDEVKQLIHRQKLAR
                     MVLENKWYESEQKKEDAFFKDLSSSRSKETITYFPKIYYRMKQGLLHIRVEITLGKYQ
                     EQLLNLEKKLESGLYCELTDKELKDSYVEYTLLYDTIANRISIEDVQAKDGRLRLMKN
                     VWWEYDKLPHMLIAGGTGGGKTYFILTLIEALLRTNAVLSVLDPKNADLADLQAVMPD
                     VYYKKEDMLTCIDRFYEEMMKRSEDMKLMENYRTGENYAYLGLPAHFLIFDEYVAFME
                     MLGTKENAAVLNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMS
                     EMGYGMMFGETTKDFFLKQIKGRGYVDVGTSVISEFYTPLVPKGHDFLKEIKKLIDSR
                     QGVQAACEAEAAETD"
     gene            7006..8217
                     /locus_tag="PB780_002764"
     CDS             7006..8217
                     /locus_tag="PB780_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDE0734730.1"
                     /translation="MRFFRQEGFSLNEETWVRDIKEKREVYGISQQKLALAAGITRPY
                     LSDIETGKAHPSEALQEAITEALERFNPDNPLEMLFDYVRIRFPTTDVKHIVEDVLRL
                     KLPYFIHEDYGFYSYTEHYYLGDIFVLVSPELEKGVLLELKGRGCRQFESYLLAQERS
                     WYEFFMDVLMEDGVMKRLDLAINDKTGILNIPHLTEKCRNEECISVFRSFKSYRSGEL
                     VRRGEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKHDIPIADAEVKNRFEIRLKNER
                     AFYAIRDLLEHDNPERTAFQIINRYVRFVDRDDTKPRSDWRISEEWAWFIGEHRGSLK
                     LTTKPEPYSFERTLHWLSHQVAPTLKLALRLDKMNHTQIVHDIITHARLTEKHEKILK
                     QQAAAAKEMVL"
     gene            8269..8490
                     /locus_tag="PB780_002765"
     CDS             8269..8490
                     /locus_tag="PB780_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734731.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLV
                     VSLVAVGLVFNADGVKDILLELFNKIIGA"
     gene            8621..9109
                     /locus_tag="PB780_002766"
     CDS             8621..9109
                     /locus_tag="PB780_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001857838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDE0734732.1"
                     /translation="MRIYIANLGKYNEGELVGAWFTPPVDFEEVKERIGLNDEYEEYA
                     IHDYELPFEIDEYTPIEEVNRLCEMVEDLPDYIQEELSELQSYFGSIEELCEHEDDII
                     CHSGCDDMADVARYYLEESGQLGELPAHLQNYIDYAAYGRDMELEGTFVVTNHGVYEI
                     LR"
     gene            9203..9595
                     /locus_tag="PB780_002767"
     CDS             9203..9595
                     /locus_tag="PB780_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDE0734733.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFKLPFPITFTQMAWFVVSVFAV
                     MLLGNLPPLSFIDGAFLKYFGVPFALTWFMCQKTFDGKKPYGFLKSVLAYLVRPKLTY
                     AGKPVKLKKEYPAQPITAVRSDIYGISD"
     gene            9579..12029
                     /locus_tag="PB780_002768"
     CDS             9579..12029
                     /locus_tag="PB780_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDE0734734.1"
                     /translation="MAYPIKYIENNLVFNHDGECFAYYELLPYNYSFLSPEQKYQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRAAQERSKQEVTGKLKDIACAKIDAQTEALISM
                     IGENQVDYRFFIGFKLLVNEQEVTMKQFRREAKTAVSDFLHEVNHKLMGDFVSMSNEE
                     IWRFQKMEKLLENKISRRFKVRRLDKDDFGYLIEHLYGQTGTAYEDYEYYLPKKRFQE
                     ETLVKYYDLIKPTRCLIEENQRYLKIEQEDGTVYAAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVTNRKALSTVRNKKKELKDLDNHAWQNDSETSTNVVDALDSV
                     NELESTLDQSKESMYKLSYVVRVTAPDLEELKRRCNEVKDFYDDLNVKLVRPFGDMLG
                     LHGEFLPASKRYLNDYIQYVTSDFLAGLGFGATQMLGETEGIYIGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALATAFVGSLGGGKSFSNNMIVYYCVLFGAQALIVDPKAERGRWK
                     ETLPEIAHEINIVNLTSEEQNRGLLDPYVIMENPKDSESLAIDILTFLTGISSRDGEK
                     FPVLRKAIRAVTNSEERGLFKVIEELRAEGTTISTSIADHIESFTDYDFAHLLFSDGD
                     VTQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     VFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDINEIKKTLAFFGVDSEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPIF
                     EDLFHAFDTRPPVRKEVE"
     gene            12116..13918
                     /locus_tag="PB780_002769"
                     /pseudo
     CDS             12116..13918
                     /locus_tag="PB780_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
     gene            14386..15390
                     /locus_tag="PB780_002770"
     CDS             14386..15390
                     /locus_tag="PB780_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDE0734735.1"
                     /translation="MKLRHLFFACSGVFVMMFSLLLLVVIVFSDEEDGGSGGNLIYGG
                     VSVSQEVLAHKPMLEKYAREYGIEEYLNVLLAIIQVESGGTLEDVMQSSESLGLPPNS
                     LSTEESIKQGCKYFSELLAAAETKGCDLNSVIQSYNYGGGFLDYVAGRGKKYTFELAE
                     SFARDKSSGKKVTYTNPVAVEKNGGWRYSYGNMFYVLLVSQYLTVAQFDDETVQAIME
                     EALKYEGWTYVYGGDSPSTSFDCSGLVQWCYGKAGIALPRTAQEQYNVTQHIPLSEAK
                     AGDLVFFHSTYNAGTYITHVGLYVGNNRMYHAGNPIGYADLTGSYWQQHLAGAGRIKQ
                     "
     gene            15407..16315
                     /locus_tag="PB780_002771"
     CDS             15407..16315
                     /locus_tag="PB780_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015552519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDE0734736.1"
                     /translation="MIQIRKEENQKKQKEKKLKVYKVNTHKKTVIALWVLLAVSFLFA
                     VYKNFTAIDIHTVHETKVIEEQILDTHKIENFVENFAEVYYSWEQSAASIDSRTNALK
                     GYLTGELQALNVDTVRKDIPVSSALTDFQIWEITEEKEQHYQVTYTVEQRITEGESGK
                     TVRSAYQVTVYVDGSGNLTIIQNPTITSVPVKSGYTPKAVQSDGTVDSITTEEINEFL
                     TTFFKLYPTATAKELTYYVNEGVLKTVGKEYIFSELVNPVYNRNGNQVTASLAVKYLD
                     NQTMTTQVSQFNLVLEKNGENWKIVK"
     gene            complement(16373..16750)
                     /locus_tag="PB780_002772"
     CDS             complement(16373..16750)
                     /locus_tag="PB780_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDE0734737.1"
                     /translation="MAKRPTAKYDFKAFGQAIKAARTERKESRKKVSDEMYISPRYLA
                     NIENKGQQPSLQIFYELVTRYDISVDQFFFPDKSAEKDTQRRQLDTLLDSMSEKGIRV
                     VTATAKEIAEVETEDRGMSRTEF"
     gene            17092..17784
                     /locus_tag="PB780_002773"
     CDS             17092..17784
                     /locus_tag="PB780_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDE0734738.1"
                     /translation="MNKVLIIDDDKELCVLIKRSVLSEDIEADFCNTGKEGLQKLKEK
                     EYQLVILDVMMPGMDGFETLEEIRKENSLPILMFTSKNDSASKVRGLRAGADDYLTKP
                     FDMDELIARIISLIRRYTRFNQTDGMTQQLEFDGLKIDIENRSVTTENGTFELPPKEF
                     DLLLYCAKNQGKILTKQQIYEEVWREEYFYDDSNIMAIISRLRKKLEVNPGSPKYIQT
                     IKGIGYRFNKEV"
     gene            17791..18708
                     /locus_tag="PB780_002774"
     CDS             17791..18708
                     /locus_tag="PB780_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDE0734739.1"
                     /translation="MEAVIFLSIIIALFSILVSCFAIRRVKKQIAEITDTLIDVKNGN
                     GNRRILSATNELVAPLAYEINEIVVSYESRLSTVRQTEETNRQLMTSLSHDVRTPLTT
                     LIGYLDAAHKGIVTGKDRDDYIETARRKAHDLKEYIDVLFDWFKLNSNEFAMDINIVE
                     AAELTRNILIDWIPIFEDKQIDYNIDIPEQPFRVKLDTDGYMRILNNLIQNVISHSHA
                     DKIEIILSKQEKNMQIRLADNGIGIEKEDLKHIFERLYKCDKGRSEKGSGLGLSIAHQ
                     LVEKMNGTITANSIQGKGTEFTLLFPLAK"
     gene            18845..19771
                     /locus_tag="PB780_002775"
     CDS             18845..19771
                     /locus_tag="PB780_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDE0734740.1"
                     /translation="MDKNYIIETKNLTKQYGSQKSVADLNIHVQKGRIYGLLGRNGAG
                     KTTTMKMLLGLTKPTSGEVKIWGKSLQGNEKKLLPRIGSLIESPGFYPNLTATENLQI
                     FATLRGIPNRHSIKNTLDLVGLPYKDKKLFSQYSLGMKQRLAIALAVMHDPELLILDE
                     PINGLDPIGIAEVRSFIRELCDTKGKTILISSHILSEISLLADDIGIIDHGALLEEES
                     LAELEQKSNKHIRFTLSDTAQAARILERTFHESHFSIQDDHNLRLHNLDLSVGKIVTA
                     FVENGLEVSEAHTCEESLEDYFKRVTGGEGIA"
     gene            19764..20573
                     /locus_tag="PB780_002776"
     CDS             19764..20573
                     /locus_tag="PB780_002776"
                     /inference="COORDINATES: protein motif:HMM:NF024141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDE0734741.1"
                     /translation="MLKLIKCEFWKLKRKRLLYALMSLSLFFPLILAYMAKAGTGADT
                     TEHYLQTRFDYVYTMMLGYGLVFLLPCLIGIIAAILFFIERDCDTSKNLRTIPVTNTQ
                     LIMAKISMLFIFSVAFCLISTLSVALFCKLFHVGMVYGMTYKIFMSLIFGVLIVAASL
                     PIVFLIIYFNKSFLLSILLAFFYSIFNWGILGTIGTSISAAKIAFLNSFPVICVMNWT
                     SGLMMDNLQKDNLLPEAYAIVPTTCHTIFIMAITVILSLWLIIRFYKKWTR"
     gene            20579..21352
                     /locus_tag="PB780_002777"
     CDS             20579..21352
                     /locus_tag="PB780_002777"
                     /inference="COORDINATES: protein motif:HMM:NF024141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDE0734742.1"
                     /translation="MRNIIKTEFLKLKRYSVIKAGIIMTTLSPLLSLFYSTANGGQTW
                     TFDYFMQQVMISNCTLFFPIIIALIAGYIITREYTDDTMKNILTIPIPYKQLLSGKLL
                     ILLLLTISFSLIGCVIALVINIIVGFPGVHFGNLLNMFIRVTGANIGIYISVLPIILI
                     FCCSANNFLGGVALAFVYGYFGTFEGTLLNYYPIKASMILVDPTCGAEYGYTYHIFPA
                     FITIVLTFLISMIILANKKKKPGTLTVGKKKKAVRKKGW"
     gene            21808..22227
                     /locus_tag="PB780_002778"
     CDS             21808..22227
                     /locus_tag="PB780_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDE0734743.1"
                     /translation="MKPSEFQTTIENQFDYICKVAMEDERKDYLKALSRQCKRETLFC
                     DMDDYTVNLFSSEDTYPSYFHTFEMDGFTVRIENSLLAEALENLDGKKRDVILRYYFL
                     GFDDTEISKILEVNRSTIQRRRHAGLEFIKKFMEDKA"
     gene            22224..>22363
                     /locus_tag="PB780_002779"
     CDS             22224..>22363
                     /locus_tag="PB780_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009009556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDE0734744.1"
                     /translation="MTAKHPMIPFPVIVRAADGDIEAVNQIVRHYSGFIASRSMRPMK
                     DE"
BASE COUNT         6427 a         4797 c         5122 g         6017 t
ORIGIN      
        1 aaaacgaaaa aaaagactgt ccattcttcc agtctttttt ccgttttttt tatttatttt
       61 ttcagtttct taatcaatgt aaaaaattct gttttgcggg atcgatttta tcattttatt
      121 gatcaagatt tttccatttc caatccaata cttctggcag atcaatacca ttttcacgaa
      181 tatagttttg atgttcttgt aatttctgat ccattttttc cgcaaaatct tctgcttgac
      241 ttcctttgat ttttaaagct gcgtcttttg ccaaatgata acgatcaagt tcatttacta
      301 cacgcatgtc aaatggtgtc gtgatatctc cattttcttt gtagccatga atcattaaat
      361 gatggttatg acgatcaaag aaaatcgtgc ggaccaatgt ttcataaccg tggaaagcaa
      421 ataagatcgg tttgtctttt gtaaaatatt gatcaaattc ttcatcgctc aacccacgcg
      481 gatcttgact tggatgacgt aatttcaata aatctacgac attaatgaac cgaattttta
      541 gttttgggaa ttcttccagc agcaaagttg cagctgctaa tgcttctaag ttagattccg
      601 ttcctgcagc agcaatgacc aattcaggtt cctcctcttt tgctgtcgaa gcccaatcaa
      661 tgattttcaa gccttcattc actaagacgg ctgcttcttc tgctgagtaa aattgagcac
      721 gcggatgttt ggaagagatg atcaaattga ttttttctga cgagtggaat gctttatcca
      781 tgactgcgag caatgtattt gcatccgcag gcagatattc acggatgaac tccgccttct
      841 tttctgccaa atgtgtcaat attcctggat cttggtggct atatccattg tgatcttgct
      901 ggaagactgt tgagcttgca atgagattta gcgaaggata atcatttcgc caagaaaggt
      961 catgtgattt ccgtatccat ttgaagtgct gagtcaacat agagtcaacc actcgcaaga
     1021 atgattcgta acttgcaaaa aaaccatgcc gtccagtcag aacgtaccct tctaagaacc
     1081 cttcatcttg gtgttccgat aattgactgt cgatgacgcg accagatggc gaaagccatt
     1141 catcattttc tggttcgatt ttttccatcc attggcgatt tgtcgttttg aaaacttgat
     1201 tcaatcggtt tgattttgtt tcatctggtc caaatatacg gaaattttct ggatttttct
     1261 caatgatctc gcgaacgaat ttaccaagtt cggtcatatc ttctgcttta acggagccta
     1321 gtttttcaaa ttgcaaagta tattttttcc aatctggcat aataagcggt cttggatcga
     1381 ttccaccatt ggtgatagga ttttttgcca ttctttggtc accagtaggc gcaatttctc
     1441 tgatttcttc taaaatacgt ccttgtgcat caaataattt ttctggttca taggatttca
     1501 gccaacggag taaagcatct gcatgatcca tatgttcttg atctacagga atcggtactt
     1561 ggtgtgctcg gaaggttcct tcaattggtt ccccgtccca ttcttttgga ccggtccatc
     1621 cttttggtgt gcgtacgatc aatactggcc attttggcat cacgacatct gttgctgtct
     1681 tcaggcgagc ttctttttgg atggatttga ttttctcgat tgcttgatcc attttttcag
     1741 ccataacagg atttaatttt tctggatcat ttccttcaat aaagaaaggc tcccagccaa
     1801 gtccgttaaa gtaatttgct aattctccat ctgatttacg tgccaagatc gttgggttgg
     1861 ctatttttgc accatttaaa tgaaggatcg gtaaaacagc tccatctatc actggattga
     1921 taaagacatt tgagaaccag cttccagcta aaggaccggt ttctgcttca ccatcaccaa
     1981 tcacgacagc agagattact tcaggattgt ctaatacagc tccgactgca tgtgaaagag
     2041 agtatccaag ctctccgcct tcatggatag aacctggtgt ttctggtgct gcatgagaag
     2101 caatgcctcc aggaaatgaa aatcgcttga ataatttttg cattcctgtc tcatcttccg
     2161 ttacttctgg atagatctct gtgtaacttc catctaaata agcattcgat accatgactt
     2221 gtccgccgtg tcctggccct tcgatataaa acatgttcaa atcatattta ttgatgactc
     2281 ggttcaaatg gacataaata aaattttggc caggaatcgt tccccaatga ccgattggat
     2341 gttttttgac atcttctggt ttcagtgtac gtctcagtaa ggggttatct ttaagataaa
     2401 gttgcccaac agaaagataa ttagcagcac gccaccaagc cgtcatttta tcaaagtatt
     2461 ctttactaga ataatccata cataacactc cttttcatta ttactcttat tctagcgcag
     2521 atactcccaa ttgaaaccta ataagcactt ctgttcattt gtttggattt tatatctagc
     2581 caaatgaaca ggtataaaaa accacgaaat ttttcctttc gctgacgttt tttacattga
     2641 tccaaaaaac ttgtcgttat cacaatggct gttccgcagt tcatttcgct cctcatcatg
     2701 cataatttgc tgaatgacgc taggccagtt aacacattgt tggaaaactg gggctggatc
     2761 gagcaattcc tttcctgatt gatccgtttt atgtaaaagt ctctgttatc tttgtcaata
     2821 tgtgaattgg tatcccagct actatgctaa tttctacagg gattatccaa aatttggatc
     2881 gtgaccaaat cgaagcggcg taatcggaaa tatcaataac ttcaacgtga tttttctttt
     2941 aactgtcaac ggaccgacaa actcaaactt ttatggtgca ggttccactg atcttctagt
     3001 tacttggcta tacctgactg tcactaaaat agattacaat ctagcttccg ttatcggtat
     3061 cttgatcttt atctatatgc aaaaatttat atactataga aaaaagaaag tcgaattttt
     3121 tacttttctt tttctctttt ttggttagct gacacgctac cagactaaag tcatggaact
     3181 cttaggtaat acgtcttaat gaaaacaaaa aaatcccgca acccctgatt tagcaaggct
     3241 ttgataagga tggtagtaga aaaccagtaa aatcaaggct ttgcagacag caggcagaca
     3301 gtgcttttat aaaaaatgaa tatgaagata gaaaaagacg catttttgag atttgcgtct
     3361 ttttctattg agctttctgc tttaccttga aacattagca gacaacaaca aacgccattg
     3421 aatggacttc cattgatggt gtttttttta ttgtctggaa aggagcagaa aaatggaaat
     3481 aacacatgaa aaagtaaaac gaaaggcggt ctatgtgtgc ccttaaatta ggaagcctgt
     3541 ttgacggaat cggcgtgttc cctcttgcgg cttcccgtaa tggaatcatt cccgtttggg
     3601 caagtgagat tgaaaaatcc ccgattgcca taacaaaacg ccatttcccc ggcatggaac
     3661 atttagggga tattacaaag atacatggag gacacatccc tcctgtccat attattacat
     3721 tcggctctcc ctgccagaac ctttctaaaa tcgggaaacg tgagggactg gctggaagca
     3781 aatcaggtct gttttttcag gctatcagaa ttatagaaga aatgaggtgt gctacaaatg
     3841 ggttatatcc agttatcgct gtttgggaaa acgtcatggg agcgttttct tcaaatgacc
     3901 ggatggattt tagagccgtg ctgcaatcat tttccgatac cgaaatttca atgcctccta
     3961 ctggaggatg ggctaacgcc ggtatggtgc gagggggaaa aactgacctg tgctggcggc
     4021 tcatggacgc ccagtattgg ggaaagcccc ggcttgttca gcgaagaaaa agaatcttcc
     4081 ttgtggcgga ttttagagga cagcgttccg cagaaatact atttaagccc cgcgatatgt
     4141 tcccgattcc tccgccttgc acagactacg ggctgcccgc cgccggagcc agtcgaatat
     4201 ctcttgataa aacaaggggg aagataccca tcatccgccc ctttcaggaa cggcgtatgc
     4261 ggagtgctgc aaaatcagga aaccagtctg ctttcctcgc aagtttcgga cggccagatg
     4321 aaccttttcc aactttgctg gcaaaagcag tcaatatgat ttcattctgg tatgaggggg
     4381 aagaaaaaga cggagttctc cgaaatttga caccggttga gtgtgagcgc ctgatgggat
     4441 taccggaggg gtggacggca tatggaaatt taggacaacc catcagtgat aatgcacggt
     4501 gcaaagcact tggaaacgca atcgccctgc cgtgtgctga ttacatcatg gcaggaatag
     4561 cagaaaccat acatgaataa gaaaggcggt tttgtatgat agagaaaaat cgttctcctc
     4621 cgttgtcggg tggaacatca acagaagcta tcgaaccaac tatattattt cagaaaggac
     4681 aggtacatct atatggaatt aaaattcgtt atccccaaca tggaaaagac attcggcaac
     4741 ttggagtttg ccggagaaga caaaacagaa cagagaagaa tcaacggacg catggcgata
     4801 ctctcccgca gcttcaatct gtattcggac gtacagagag cggatgatat tgtagttatc
     4861 ctccctgccg aagccggaga aaaacatttt gactttgagg aacgtgtgaa gctcgtcaat
     4921 ccccgtatca ccgcagaggg ctataaaatc ggcacaaggg gctttaccaa ctacattttg
     4981 cacgctgacg atatggtaaa agcgtaagga aaaggaggac atcaaactat gagattagca
     5041 aacggaatcg taattgacaa agaagcaaca ttcggggcat tgaaattttc tgccctccgc
     5101 cgtgaggttc acctccagaa tgaagacggc tcggtatcag aagaaatcaa ggagcgtacc
     5161 tatgacttaa aatccagagg acagggacgt atgattcagg tttccatccc tgccagcgtg
     5221 ccgttaaagg agttcgatta caacgcagag gtagaactta tcaatcctgt ggcagatacc
     5281 gtggcaacgg ctacctttca gggggcagag gtggactggt acatcaaggc ggaggatatt
     5341 gtcctgaaaa aaggggctgc catgaatcca cagccgccga agaaagacgc tccccctgta
     5401 aaataacagg aatataagaa gaaagcgaaa ggaggattgt tcccatgaag cagcttttcc
     5461 accgtggaaa acgtatccgt cccacggata aagaccttgt attccatact gcccttgctt
     5521 ctctgttccc tgtcttcctg ctggttgtcc tgctgtttca tatccggcag attgccggaa
     5581 ccaactggca ggaagagtcc ctctcccaga tagtacagga tgtaaacatc ccttacctgt
     5641 tattcagtat gggcgtggca gggttggtgt gtcttacagc cgttcttctg ttctggcgat
     5701 accgcaggga tgaagtaaag cagctcatcc accgccaaaa gctggcaagg atggtgttgg
     5761 aaaacaagtg gtatgaatca gagcaaaaaa aggaagacgc ttttttcaag gacttgtctt
     5821 ccagccgttc caaagaaacc atcacttact ttcctaaaat ctactaccgg atgaaacagg
     5881 gcttgctcca tatccgtgtg gaaatcacct tagggaaata tcaggaacag ctcttaaact
     5941 tggagaaaaa gctggaaagc ggcttatact gtgagctgac ggataaggaa ttaaaggatt
     6001 cctatgtgga gtacaccctg ctttatgata ctattgccaa ccgtatctcc attgaggacg
     6061 tgcaggcaaa ggacggcagg ctccggctta tgaaaaatgt ctggtgggag tatgacaagc
     6121 tccctcatat gctcattgcc ggaggaaccg gcggcggaaa gacttatttc atcctgaccc
     6181 ttattgaagc cctgctccgc accaacgccg tcctgtccgt gttagacccg aagaacgccg
     6241 accttgcgga tttacaggcg gttatgccgg atgtgtacta caaaaaggaa gatatgctca
     6301 cctgtattga ccgtttctat gaggaaatga tgaaacgcag cgaagacatg aagctcatgg
     6361 agaattaccg gacaggggaa aactatgctt atctggggct tccggcacat ttccttatct
     6421 ttgatgaata tgtggcattt atggaaatgc tcggcacaaa ggaaaatgct gctgtcctca
     6481 acaaattaaa gcaaatcgtt atgcttgggc ggcaggctgg cttcttcctg attctcgcct
     6541 gccagcgtcc ggacgcaaag tatctggggg acggaatccg tgaccagttc aatttccgtg
     6601 tggctctggg gcggatgtcg gaaatgggct atgggatgat gttcggggaa accacaaagg
     6661 acttcttcct caagcagata aaggggcgtg gctatgtgga tgtgggaacc agcgttatct
     6721 cggagtttta cacgcccctt gtgccaaaag gacacgattt cctcaaagaa ataaaaaaac
     6781 tcatagacag caggcaggga gtgcaggcgg cgtgcgaagc ggaagccgca gaaacggact
     6841 gacctgctgt ggctggtgtg ccgcaaggca cgccagcagg aacccctcgt atctaacaga
     6901 ggggtacaaa tcgacaggca acaaccaaac aacagggcag aatcccacgg tacacaaggg
     6961 attttcccat atccggcgtg tgtcaacaag gttctgaaag ttgacatacg cttttttaga
     7021 caggagggat tttcactgaa tgaagaaaca tgggtacggg acatcaaaga aaaacgggaa
     7081 gtttacggta tctcacaaca gaaacttgcg ttagctgccg gaatcacccg tccgtacctg
     7141 agcgatattg aaacgggcaa ggctcaccca tccgaagcat tacaggaagc catcacggaa
     7201 gctctggaac gcttcaatcc agacaatccc cttgaaatgc tctttgacta tgtgaggatt
     7261 cgcttcccca ctacggatgt gaagcatatc gtggaagacg tgctgcggct aaagctgcct
     7321 tactttatcc atgaagacta cggattttac tcttacacgg agcattacta tctgggtgat
     7381 attttcgttc tggtatcgcc ggaactggaa aaaggggtgc tgctggaact gaaagggcgt
     7441 ggctgccgcc agtttgaaag ctatcttctg gcacaggaac ggagctggta tgagtttttt
     7501 atggacgttc tcatggagga cggcgtgatg aagcggcttg accttgccat caatgacaaa
     7561 acgggaatcc tgaacattcc ccatctgaca gagaagtgcc ggaatgagga atgtatctcg
     7621 gtcttccgca gcttcaaaag ctaccgcagc ggcgaactgg tacggcgtgg ggaaaaagaa
     7681 tgtatgggga acaccctgta tatcggctcc ctccaaagtg aggtgtactt ctgcatttat
     7741 gaaaaagact atgagcagta caaaaagcat gatattccca ttgcggacgc agaagtaaag
     7801 aaccgctttg aaatccggct gaaaaatgag cgtgcctttt acgccatccg tgacctgtta
     7861 gaacatgaca atccagaacg gacagccttt caaatcatca accgctatgt ccggttcgtg
     7921 gacagggacg atacgaagcc ccgttctgac tggcgtatca gtgaggaatg ggcgtggttt
     7981 atcggggaac acaggggaag cctgaaactg acaaccaaac cggaacccta ttcctttgaa
     8041 cgcaccctgc actggctctc ccatcaggta gcccccacgt taaagctggc tctccgtctg
     8101 gataagatga accacaccca gattgtccat gacatcatca cccatgcaag gctgacggag
     8161 aagcatgaaa aaatcctgaa gcagcaagcc gccgctgcaa aggagatggt gctttaacgg
     8221 cggcaatctt ggaagctaag aaatcttaca gcaaagaaag gagcttttat gaatttcgga
     8281 cagaatcttt acaactggtt tttatccaac gcccagtccc ttgtgctgct ggcaatcgtg
     8341 gtgattggac tgtacctcgg atttaagagg gaattttcca aacttatcgg ctttttagtg
     8401 gtgtcccttg tagcggttgg tctggtcttc aatgcggacg gtgtaaagga catcttactg
     8461 gaactgttca ataagattat cggtgcatag gaaaaactat atcacacttc cgttatggag
     8521 caagctctga ttgcttcatg gcggaagtac actaggggct tggggagcgt aactccccaa
     8581 aaggagcatg acagagaatg gaggtgacac tgtggaagaa atgcggattt acattgccaa
     8641 cttaggaaaa tacaatgagg gcgaactggt gggggcatgg ttcacgccgc cggtggactt
     8701 tgaggaagtc aaggaacgta tcggcttgaa tgatgaatat gaggaatacg ccatccatga
     8761 ttatgagctg ccctttgaga ttgacgaata tacccccatt gaggaagtca accgcctgtg
     8821 tgaaatggtg gaggacttac cggattacat tcaggaggaa ctatcagagc tgcaatccta
     8881 ctttggcagt atcgaagaac tctgtgagca tgaagacgat attatttgcc attccggctg
     8941 tgacgatatg gcggatgtgg ctcgctacta tctggaagaa agcggacagc ttggcgaact
     9001 tccggcacac ttgcaaaact atatcgacta tgcagcctat gggcgtgaca tggaattgga
     9061 gggaaccttt gttgtcacga accacggcgt atatgaaatc ttacggtagc cccgtctgta
     9121 tctctttcag gaaatacagg cggatttttt tattcagggg cggctctttt gctgccccca
     9181 tcttttagaa aggacggaca acatgaagaa aatacgaagc tataccagta tctggtcggt
     9241 ggaaaaggta ctctattcca tcaatgattt taagctgcca ttccccatca cgttcacaca
     9301 gatggcgtgg ttcgtggtat cggtgtttgc agttatgctc ttggggaacc tccctcccct
     9361 ttcgttcatt gacggggcat ttctgaaata tttcggcgta ccctttgccc tcacttggtt
     9421 catgtgccag aagacctttg acgggaaaaa gccttatggc ttcctgaaat ccgtactggc
     9481 atatctggta cgcccgaaac tgacctatgc aggaaagccc gtgaagctga aaaaggaata
     9541 tccggcacag cccatcacgg cagtaaggag tgatatttat ggcatatccg attaaatata
     9601 tcgaaaataa ccttgtgttc aaccatgacg gggagtgctt cgcttactat gagctgcttc
     9661 cctacaacta ttccttttta agccccgaac agaaatatca ggtacacgat tccttccggc
     9721 agcttatcgc ccagaacagg gacgggaaga ttcacgccct gcaaatcagc acagaatcca
     9781 gcatacgggc ggcacaggaa cgctccaaac aggaagtgac gggcaagtta aaggacattg
     9841 cctgtgcaaa gattgacgca cagaccgaag ccctgatttc catgataggg gaaaatcagg
     9901 tggactaccg ctttttcatc ggctttaagc tgcttgtcaa tgagcaggaa gtcaccatga
     9961 aacagttccg cagggaagca aagaccgctg tttccgactt cctccatgaa gtcaaccaca
    10021 agctcatggg ggattttgtt tccatgagca atgaggaaat ctggcggttt cagaagatgg
    10081 aaaaactgct ggaaaataaa atctcacgcc gcttcaaagt ccggcggctg gataaggacg
    10141 attttggcta cctgattgag catttatacg ggcagaccgg aaccgcctac gaagattatg
    10201 agtattacct gccgaagaag cggtttcagg aagaaacgct ggtgaaatac tatgacctca
    10261 tcaaacccac ccgttgtctg atagaggaaa accagcggta tctgaaaatc gaacaggaag
    10321 acgggacagt gtatgccgcc tactttacca tcaacagcat tgtgggggag ctggacttcc
    10381 catcctcgga aatcttttac tatcaacagc agcaatttac ctttcccatt gatacctcta
    10441 tgaatgtgga gattgtcaca aaccggaaag ccctctccac ggtgcggaac aagaaaaaag
    10501 agctgaaaga tttggacaac cacgcatggc agaatgacag cgaaaccagt acaaacgtgg
    10561 tggacgcttt agacagcgta aatgagctgg aatccacctt agaccagagc aaggaatcca
    10621 tgtacaagct gtcctatgtg gtgcgtgtga cggctcccga cttggaagaa ctgaaacgcc
    10681 gctgcaatga ggtgaaagat ttttatgacg atttgaacgt gaaactggtt cgcccctttg
    10741 gagatatgct ggggctgcat ggggaatttc ttcctgccag caaacggtat ctgaacgatt
    10801 acatccagta tgtcaccagt gacttcctcg ctggtcttgg cttcggggca acccagatgt
    10861 taggagaaac ggagggtatt tatatcgggt acagccttga tacaggaaga aacgtgtact
    10921 taaagcctgc ccttgccagt caaggggtaa aaggctccgt caccaacgcc cttgctaccg
    10981 cttttgtcgg ctccctcggc ggtggaaaat cattcagcaa caacatgatt gtctactact
    11041 gtgtattatt tggggcacag gctctcattg ttgacccgaa agcggaacgg ggacgctgga
    11101 aagaaaccct gccggaaatc gcccatgaaa tcaatatcgt aaacctgacc tcggaggaac
    11161 agaacagggg cttactcgac ccatatgtga ttatggagaa cccaaaggat tccgaatcac
    11221 tggcaatcga catcctgact tttttaacgg gcatttccag ccgtgacggg gaaaagttcc
    11281 ctgttcttcg gaaagccatc cgtgcggtga caaacagtga agaacggggg cttttcaaag
    11341 tgattgagga acttcgggcg gagggaacca ccatcagcac cagcatagcc gaccatatcg
    11401 aatcctttac ggactatgac tttgcacacc tgctcttttc ggatggggat gtcacacagt
    11461 ccatcagcct tgaaaagcag ctcaatatta ttcaggtggc ggatttggta ctgccggata
    11521 aggaaacctc gtttgaagaa tacaccacaa tggagctgct ttccgtggca atgctcattg
    11581 taatcagcac ctttgccctc gacttcatcc acactgaccg cagcgtgttt aagattgtgg
    11641 atttggacga agcatggagt ttcttacagg tggcacaggg caagaccctc tctatgaagc
    11701 tggttcgtgc cggacgtgcc atgaacgcag gcgtttactt tgtcacccag aacacggacg
    11761 atttgctgga tgaaaagctg aaaaacaatc tgggcttgaa atttgcgttc cgttccacag
    11821 acatcaacga aatcaaaaag accctcgcct ttttcggggt ggattccgaa gatgaaaaca
    11881 accagaagcg gctccgtgac ttggaaaacg gacagtgcct tatcagtgat ttgtacgggc
    11941 gtgtgggcgt gatacagttc catcctatct ttgaagacct gttccacgcc tttgatacca
    12001 gaccgcccgt aagaaaagag gtggaatgat ggtgatacat agaacttcca accgaccgca
    12061 attcccagtt cctcatcaca caaaaaaggg cttgtcttgg aagacggcag gaaaagtgat
    12121 tggcagggtg cttctggcac tcctgctcat cctcctgctg ctggcagtct tcggcacagc
    12181 cgcccacgct gccggattgg tggatgatac ggtggacgct gccaacgaat acagcaaata
    12241 tcctctggac aactaccagc ttgattttta tgtggacagc ggatgggact ggctcccgtg
    12301 gaactggctg gacggtatcg gaaaacaggt gatgtacggg ctgtatgcca tcacaaattt
    12361 tatctggaca atcagcttgt acctttccaa cgccacaggg tatctgattc aggaagccta
    12421 ctcgctggac ttcatttcct ctacggcgga ttctatcggg aaaaatatgc agacccttgc
    12481 tggcgttacc acaggcggct tgtcctcgga gggattttat atcgggttcc tgctgattct
    12541 cattctggtg gtggggattt acgttgccta cacagggctt atcaaacggg aaaccacaaa
    12601 agccatccat gccgttgtca atttcatggt ggtgttcgtg ctgtccgctg cctttatcgc
    12661 ttatgcccct gattatatcg ggaaaatcaa tgaattttcc gcagacatca gtaatgccag
    12721 cctgaccctt ggtacaaaga ttgtgctgcc taactcggaa agtcaaggaa aagacagcgt
    12781 ggatttgata cgggacagcc tgttttccat tcaggtcaag cagccgtggc tcctgctgca
    12841 atacggcaat tcggatgtgg aaagcattgg agctgaccgt gtggaaaacc tactttcaac
    12901 cagtccagac gaaaacaacg gacaggacag ggaagaaatc gtagtggagg aaatcgaaga
    12961 cagggaaaac acgaacctta ccatcacaaa gaccatcaac cgattgggaa ccgtcttctt
    13021 cctgttcatg ttcaatatcg gtatctccgt ttttgtattc ctgctcacgg ggataatgat
    13081 tttctcacag gtgcttttta tcatttacgc catgttcctg cctgtgagct ttctccttag
    13141 catggttccc tcttttgagg ggatgtcaaa gcgtgccatt acgaaactgt tcaacactat
    13201 ccttaccaga gccggaatca ccctgattat cacggtagcg ttcagcattt ccaccatgct
    13261 ctacaacctg tctggggaat atccgttctt cctgacggcg ttcttgcaga tagtgacttt
    13321 tgccggaatc tatttcaagc tgggggattt gatggggatg ttctccttac agagcagcga
    13381 ttcccaaagc atggggagcc gtatcatgcg aagaccccgt atgctcatgc acgcccatat
    13441 gcaccgttta cagcataagt taggacgttc cgtggcggct cttggagctg gaacggctgc
    13501 ataccacgca ggaaaacagg ctggctcaga ccagagaaat gcttcccact ccggctcttc
    13561 caaacggaca caggcagacc acagcagacc agacggacag gcagcaccgg aaaaagaatc
    13621 cgtatggaaa cgggctggct ctgctgtggg tgcggcggca gataccaaag ataagatagc
    13681 cgatactgcc ggacagcttc gggagcaggc aaaggatttg cctgtcaatg caaagtacgc
    13741 cctttaccac gggaaaacac aggtatcaga gggtgtaagg gattttactt ccagcgtgac
    13801 ccagacccga actgccagag ccgaacagcg gaacacacag gcagaaagcc gcagacagac
    13861 cattgcagaa cgcagggcgg aactggaaca ggcaaaacag ccgcaacaaa gagcttccga
    13921 agcccccaaa ggggcggctc ctgtccatga acgccctgtt actgaaaagc agccagaaga
    13981 tttccgacat catgcaggaa aaccagccat gcagcctgct tccccatcca tcagggaacg
    14041 gggacaggtt ccttatgggg gaactgtggc agaacggtct tcggttccgg tggtaaaagc
    14101 ggcttccatc caccatgaac agacaccgcc tgtaaggaca gaacgccaga tagtccctcc
    14161 ggcttcccca gacaaaccag atgaaagaca aaagacaact actacacctg ctgctcccca
    14221 tccgacaaga cctgtccaga atgatacggc tcctgtgata ccggaaagaa aacgggctgc
    14281 ccctgcggta aaagaatcca actttaccat ccggcgtacc accgccagaa aggagtggac
    14341 aaaaacagta acagcggctt ccaaacaaaa gaaaggggag aagccatgaa gttaagacac
    14401 cttttctttg cctgttccgg cgtgttcgtg atgatgttct ccctgctcct tttggtggtg
    14461 attgtcttct cggatgagga agacggagga agcggcggaa accttatcta tgggggtgtg
    14521 agcgtatcac aggaagtcct cgcccataag cctatgctgg aaaagtatgc aagggaatat
    14581 ggcatagagg aatacttaaa tgtgctgctc gccatcattc aggtggaatc cggcggcaca
    14641 ctggaagacg ttatgcagtc ctcggaatct ctggggcttc ccccaaactc ccttagcaca
    14701 gaggaatcca tcaaacaggg ctgcaaatat ttctcggaac tgctcgcagc ggcagaaaca
    14761 aaaggctgtg acttaaacag tgtgattcag tcctacaatt atggcggcgg tttcctagac
    14821 tatgtggcag ggcgtgggaa aaaatacact tttgaactgg cagagagctt cgcaagggac
    14881 aaatccagcg gaaagaaagt cacctacacc aaccctgttg cggtagagaa aaacgggggc
    14941 tggcggtatt cctatggaaa tatgttctat gtcctcttgg tatcgcaata cctgaccgtg
    15001 gcacagtttg atgatgaaac ggtacaggct atcatggagg aagccttaaa gtatgagggc
    15061 tggacgtatg tgtacggggg cgattcccca tccacttcct ttgactgttc tggactggtg
    15121 cagtggtgct atggcaaggc aggaatcgcc ctgccacgga cagcacagga acagtacaat
    15181 gtgacgcagc atatccccct gtccgaagca aaagcagggg atttggtctt tttccactcc
    15241 acctacaacg ccggaaccta catcacccat gtggggctgt atgtgggaaa taaccggatg
    15301 taccatgccg gaaatcccat tggctacgca gacctgacag gctcttactg gcaacagcac
    15361 cttgccggag ccggacgaat caaacaataa gaaaggatga accgatatga ttcagattag
    15421 aaaagaggaa aaccagaaaa agcagaaaga aaagaaactg aaagtttaca aagtcaatac
    15481 ccacaaaaaa accgtgattg ccctgtgggt gctgctggcg gtgagcttcc tatttgccgt
    15541 ctataagaat ttcacggcga tagacattca tactgtccat gaaaccaaag taattgagga
    15601 acaaatcctc gacactcaca agatagagaa ttttgtggag aatttcgcag aagtctacta
    15661 ttcatgggaa cagtccgccg cttccattga tagccggaca aatgctttaa aagggtatct
    15721 caccggagaa ctgcaagccc tgaatgtaga tactgtccga aaggacatcc ccgtatcgtc
    15781 tgccctgacc gattttcaga tatgggaaat cacagaagaa aaggagcagc attatcaagt
    15841 gacctataca gtggaacagc gtatcacaga gggagaatcc ggtaaaaccg tccgttccgc
    15901 ctatcaggtg acggtctatg tggacggctc tggaaacctg acgattatcc agaatccaac
    15961 cattaccagc gttcccgtaa aatccggcta tacgccaaaa gcggtacaaa gtgacggtac
    16021 ggtggattcc atcactacgg aggaaatcaa cgaatttctc accacctttt tcaagctgta
    16081 ccctacggca accgcaaagg aactgaccta ttatgtaaat gagggtgtgc tgaaaactgt
    16141 gggaaaagag tatattttct cggaactggt aaatccggtt tataaccgaa acggaaatca
    16201 ggtgacagct tcccttgcag taaaatatct ggataaccag accatgacaa cacaggtatc
    16261 acagtttaac cttgtactgg aaaagaacgg ggaaaactgg aagattgtga aataagctat
    16321 ataaaacaga agacacattg tatttcctga aagattacaa tgtgtcttca cgttaaaatt
    16381 ctgttctgga cattcccctg tcctctgttt cgacttcggc tatctctttt gctgtggcgg
    16441 tgactacccg tattcctttc tcactcatac tatcaagcag tgtatcaagc tgcctacgtt
    16501 gtgtatcttt ttccgctgat ttatctggga aaaagaattg gtctacggat atatcatagc
    16561 gtgttacaag ctcataaaaa atttgtaaac tcggctgttg tcctttattc tcaatattag
    16621 caagataacg tggtgagata tacatttcat cacttacttt tttacggctc tcttttcgtt
    16681 cggttctcgc tgctttgata gcttgtccga aagccttaaa gtcatattta gctgttggtc
    16741 tttttgccat atttattcgc ccatatacat tctactgttc ctctttcata ctgaatacgt
    16801 ctatacagtt cctatttata gccataataa ttcatatgct tgcacttgct attattcgta
    16861 tgcccgacta taattatact gatatgattt gtagaaagga cttgggtgtt atggaatata
    16921 tgtcttgccc cgaagcagcg aagaaatggg ggatttctga acggcgtgta cagatactat
    16981 gcagagagaa ccgcatcccc ggtgtttcaa aacttgggta tatgtggctg ataccaaagg
    17041 acgcggaaaa accgattgac gggagaacaa aacaaggaaa ggagctacac catgaataaa
    17101 gttttgatta tagatgatga taaagagctt tgtgtgctga ttaagcgaag cgttttatca
    17161 gaagatatag aagctgattt ttgtaataca ggaaaagagg gactacaaaa attaaaagaa
    17221 aaagaatatc aacttgtgat actggacgtt atgatgcccg gtatggacgg atttgaaaca
    17281 ttggaagaaa tcagaaaaga aaacagtctg ccgattttga tgtttacctc taaaaatgac
    17341 agcgcttcca aagtgcgcgg cttacgcgca ggagccgatg actatttaac aaagcctttt
    17401 gacatggacg aactaatagc ccgtattatt tctcttatac ggcgttatac ccgtttcaat
    17461 caaacagacg gaatgacaca gcagcttgaa tttgacgggt tgaaaattga cattgaaaat
    17521 cgttctgtta ctacggaaaa tggaactttt gaactgcctc caaaagaatt tgacttactc
    17581 ttatactgcg cgaaaaatca agggaaaatt ctgacaaagc aacagattta tgaagaagtg
    17641 tggagagagg aatatttcta tgatgacagc aacattatgg cgattatcag ccgacttcgc
    17701 aagaaattag aggtaaatcc cggaagtcca aaatatattc aaacgattaa aggtatcggt
    17761 tatcgtttta acaaggaggt ttgattttct atggaagcag tcatttttct atccataata
    17821 attgcacttt tttccattct tgtttcctgc tttgctattc gacgtgttaa aaaacagata
    17881 gccgagataa cagacacatt gattgatgta aaaaacggaa atggaaacag gcgtatttta
    17941 tctgcaacaa atgaactggt agcccctctt gcctatgaaa taaatgagat tgttgtgtct
    18001 tatgagagca ggctttctac tgtccggcag acagaagaaa caaaccgaca gcttatgaca
    18061 agtctttccc atgatgttcg gacaccgctt acaacgctta ttgggtatct tgacgctgca
    18121 cataaaggaa ttgttacagg aaaagacagg gacgactata tcgaaacagc ccgccgcaaa
    18181 gctcacgatt tgaaagaata tattgatgtt ctttttgact ggttcaaatt aaattctaat
    18241 gagtttgcta tggatataaa tattgttgaa gctgcggagc tgacacgaaa tatcttgatt
    18301 gactggatac ccatatttga agataagcag atagattaca acattgatat tcccgaacag
    18361 cctttccggg taaaacttga tacggacggc tatatgcgga tattgaacaa tcttattcag
    18421 aatgtaattt ctcacagtca tgcggataaa atagaaataa tcctgtcaaa gcaggaaaaa
    18481 aatatgcaaa tccgattagc agataatgga atagggattg aaaaagaaga tttgaagcat
    18541 atttttgaac gactctataa atgcgataag ggacggtctg aaaaaggcag cggtcttggg
    18601 ctttctatcg cacaccaact tgtagaaaag atgaacggca caataacagc aaatagcata
    18661 cagggaaaag gaacagaatt tactttgctt tttcccttag caaagtaagg ttctcccgtc
    18721 cttttgacgg gagccttaat tatttgggga gaaatatttc aaattgcaag gttaatgcaa
    18781 ggttgcggca aggttaatgc aaggttggta tgatagaata gtttacagaa caggaggttt
    18841 gaatatggac aaaaattata tcattgaaac aaaaaatctt acaaagcagt acggctcaca
    18901 aaagagtgtc gctgacttaa atattcatgt gcagaaaggg agaatttacg gactgcttgg
    18961 aagaaacgga gccggaaaaa cgactactat gaaaatgcta cttggattga caaagccaac
    19021 ttccggcgaa gttaaaatat ggggaaagtc tttgcagggg aatgaaaaga aattgttgcc
    19081 ccgtatcggt agtctgattg agtcgcccgg tttttaccct aatttgactg ctacggaaaa
    19141 cttacaaatt tttgctacct tacggggcat acccaaccgt cattcaatta aaaacacatt
    19201 ggatttggta ggattgcctt ataaagacaa aaaactgttt tcccagtatt ctcttggtat
    19261 gaaacaacga cttgcgattg cacttgcagt tatgcacgac cctgaacttc tgattttaga
    19321 tgaacctatc aatgggcttg acccgattgg aattgcagaa gtacgttctt ttattcgcga
    19381 gctttgcgat acaaagggaa aaacaatttt aatttccagt catatccttt cggagatttc
    19441 tttgctagca gatgatattg gaattatcga ccatggcgca ctattggaag aagaaagtct
    19501 tgcggaactg gaacaaaaaa gcaacaaaca tatccgattt actctttctg ataccgcaca
    19561 ggcagcaaga attttagagc gtactttcca tgagagccat ttctctatac aggacgacca
    19621 caatttacgc ttgcataatc ttgatttgtc cgtaggaaaa atcgtgactg cttttgtaga
    19681 aaatggattg gaggtatcag aagcccatac ttgtgaagaa agtcttgaag attacttcaa
    19741 gcgtgtgaca ggaggcgaag gaattgctta aactgattaa gtgcgaattt tggaaattaa
    19801 agcgaaaaag actgttgtat gcactgatgt cattgtcgtt attttttccg ctgattttgg
    19861 catatatggc aaaggcggga actggtgcag atacaacgga acactatctt caaacccgat
    19921 ttgattatgt ctatacaatg atgttgggat atggtttagt ttttttgctt ccatgtctaa
    19981 ttggtatcat tgccgctatt ttgtttttta tagaaagaga ttgcgatact tccaaaaacc
    20041 ttagaacaat tccagttaca aatacacaac tcattatggc aaaaatttct atgctcttta
    20101 ttttcagcgt tgcattttgt ctgataagca cattgtctgt tgcgttgttt tgtaaattgt
    20161 ttcatgtagg aatggtttac ggtatgactt ataagatatt tatgagcctt atttttggag
    20221 ttcttatcgt agcagcttct ttaccgattg tatttttaat tatttacttt aacaaaagtt
    20281 ttttactttc tatcctgctt gcattttttt attccatttt taactggggc atactgggaa
    20341 cgattgggac atctataagt gctgccaaaa ttgctttctt aaattctttt ccggttatat
    20401 gtgttatgaa ctggacaagc ggcttaatga tggataattt gcaaaaagat aatcttttgc
    20461 cggaagccta tgcgattgta ccaaccactt gccatacaat ttttattatg gcaattacag
    20521 ttatactttc tttgtggctg ataattcgct tttataaaaa atggacgagg taaaagatat
    20581 gagaaatata ataaaaactg aatttttaaa attaaaacga tattctgtaa taaaagccgg
    20641 aattattatg acaacacttt ctccgttgct gtcactgttt tattccacag ccaacggtgg
    20701 acagacatgg acgtttgact attttatgca acaggttatg attagtaact gtaccttatt
    20761 tttcccgatt attattgcgc tgattgcagg ttatatcata acaagggaat atacagacga
    20821 tactatgaag aatatattaa caattcctat tccatataaa cagttacttt ctggaaaatt
    20881 acttattcta ttgctgttga caatcagctt tagtcttata ggttgtgtga tagcattagt
    20941 aatcaatatt attgtcggat ttccgggagt tcattttggc aatctactca atatgtttat
    21001 tcgtgttact ggggcgaata taggaattta tatttcagtc ttgccgatta ttctgatatt
    21061 ctgttgttcg gcaaataatt ttttgggtgg agttgcacta gcttttgtgt atggatattt
    21121 tggaactttt gaaggaacac tgttaaatta ttatccgatt aaagcaagca tgattttagt
    21181 agaccctact tgtggtgcgg aatatggtta tacttatcac attttccccg ctttcattac
    21241 aatagttttg actttcttga tttctatgat tattcttgca aataaaaaga aaaaacctgg
    21301 tacattgact gttggtaaga agaaaaaagc agttagaaaa aagggttggt aaataatttg
    21361 attgatagta cctgccgaac gacggcaaaa gaaaaaaagc tgtcgcagag cagatacact
    21421 ttcacgcgcc tttgaagcgg tggctttaat ttttaaagcc gccgcttctt tttgtctact
    21481 gctacctata acataaatta gcggcattta ggagggctgc cgctatggga atttctttgc
    21541 tgctatacag catacatagg ttgcattcgt taaaaaaagt atagctcctc cactgtggaa
    21601 gtccgacttt gaatatgaac tgacaagtca tatttattct actgttattt ctcatgcact
    21661 catttttatt ttaaaatggg tgtatttttt tattcaaaaa tgcaactaca ccccctgtcc
    21721 tgtcgggact aagagcgaaa aggggggaag aagcagaaat ttctatcaac tcgctccctt
    21781 tcaaggctgg aaagggggtg agcaatgatg aagccatctg aatttcagac aaccatagag
    21841 aaccagtttg attacatttg taaagtcgct atggaagatg aacgcaaaga ctatctcaaa
    21901 gcgttgtcca ggcagtgcaa acgggaaacc ctgttttgtg atatggacga ttacaccgtc
    21961 aatctgtttt cttccgaaga cacctatcca tcctatttcc atacttttga aatggatgga
    22021 tttactgtcc gcattgaaaa cagcctgtta gcagaagcgt tggaaaatct ggacggaaag
    22081 aaacgtgacg ttatcctgag atattatttc ctcggatttg acgatacgga gatttcaaaa
    22141 attctggaag tcaaccgttc taccatccag agaagaagac acgccggact ggaatttatc
    22201 aaaaagttta tggaggataa agcatgacag caaagcaccc tatgattccg tttcctgtga
    22261 ttgtaagggc tgcggacggc gatattgaag cagtcaacca gattgtacgc cattacagcg
    22321 gttttattgc cagccgttcc atgcgtccca tgaaagacga ata
//
LOCUS       DAMEET010000266         7679 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.guided.103, whole genome shotgun sequence.
ACCESSION   DAMEET010000266 DAMEET010000000
VERSION     DAMEET010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.guided.103"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1..998)
                     /locus_tag="PB780_002780"
                     /pseudo
     CDS             complement(1..998)
                     /locus_tag="PB780_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
     gene            1489..2427
                     /locus_tag="PB780_002781"
     CDS             1489..2427
                     /locus_tag="PB780_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDE0734745.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAV
                     KKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRD
                     MVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIRE
                     ISPIDLTELMKSTLKANEKQLQKQIDFWQREFRFWK"
     gene            2557..3204
                     /locus_tag="PB780_002782"
     CDS             2557..3204
                     /locus_tag="PB780_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-7 chloramphenicol O-acetyltransferase"
                     /protein_id="HDE0734746.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNS
                     SFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
     gene            complement(3897..4835)
                     /locus_tag="PB780_002783"
                     /pseudo
     CDS             complement(3897..4835)
                     /locus_tag="PB780_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(4835..5452)
                     /locus_tag="PB780_002784"
     CDS             complement(4835..5452)
                     /locus_tag="PB780_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDE0734747.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            complement(5466..5636)
                     /locus_tag="PB780_002785"
     CDS             complement(5466..5636)
                     /locus_tag="PB780_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734748.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            complement(5984..7474)
                     /locus_tag="PB780_002786"
     CDS             complement(5984..7474)
                     /locus_tag="PB780_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HDE0734749.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
BASE COUNT         2395 a         1306 c         1227 g         2751 t
ORIGIN      
        1 aatacaatat tgttgccaca cttaggacat tttcctacaa tacttttttc tgcttctttt
       61 tctttctgtt cctggtaatc agaaaaattt agtttttcta tatcgttagg tacagcttcc
      121 agtaaatgaa caatgaattt tttgatattc gtaataaagt tctcttgatt gccttctctt
      181 ttaccgattt tttttaaata cgtttcccat ttagccgtca tttcagcact cgttaaaagg
      241 tgctgacttt caactgcctg gcacaataat tttccttttt cagttacaac aagcttattc
      301 ttaatcactt ggatatattc tttttgtttt aacgcttcaa taatacttgc tcttgtcgct
      361 tctgttccaa tcccctcaac ttcttgtaaa atcttgattg cttcttcatc atcaaccgtt
      421 ttattcgccg ttttcatagc agttaataat gtaccctctg taaaagcttt tggcggttgc
      481 gtttcttttt caacactctt aacaccaact tcggcacgtt cgccaatgat aacgagtggt
      541 aacgtttgaa cgtcctcttt ttcttctttg gtttgttgtt tgaaaagaat tttccaccct
      601 tcttgctttg gtgtctttcc gatcgattga aacagcaaat cagctacttt cgtttgtatc
      661 ttggtttctt catacaagta atcaggtaaa aacatggcaa cggtcgtttt aacgactaaa
      721 gcataaattt ttcgttgtaa atcgtccatt ttcgctaatg cagattcggt aggtacttgt
      781 tttgttggga taatggcgtg gtgttcctgt accttactac cgtccacata acgctttcta
      841 ggctctgttt gaaccatttc aagatcaagt cctaaaaaac cgctatattt gccaaaattc
      901 gcttttaaat aagcaaattc gttctctgta ataaatggtg tatctgttct tggataactc
      961 aataattttg cttcatacag tccttgcata gcttttaaac gtaaaatttt gcgttctaag
     1021 acgttttaaa tatatctgcg tatattttta tgtgttttat cttttcgtcg gcaaatgcgt
     1081 taaaatttgc gaaataaaat agctcaaggg cgaacgcagt gagtgcattt acccttgatc
     1141 tatttttttg agtaaattag cttttgaaac cgacggaaag gaaacacaaa acttttcaga
     1201 ggcaaggggt ttttaagggg cgcttttagc cccttgaatt tatttgcttt aagatatatt
     1261 tagttttaaa atagttttaa cgaaaaaaag acataaaaaa agacgagcaa cgctaccaac
     1321 gttctcgccc ggaataacaa accacacttg ttattcattc gatgattagt atatcaattt
     1381 ttgattttga atacaaaaca aaatggacga atggatacag gcgaaattta ttttgcgatt
     1441 ccactcgtcc ttttttcgtt ccaaaaatta ttttaggagc gataaaaaat gaagaaaatt
     1501 gatgaaaaat tagctgcaga aatctcacta aaaaaaccgg ccgtctctaa tagccggtta
     1561 agtaatagcc ggttaagtgg tcaaactttg ggaaaatctc aacccgcatt aagttttgat
     1621 gccatgacaa tcgttggaaa tttgaacaaa actaatgcta aaaagctatc tgactttatg
     1681 agtgtagagc cacaaatacg actttgggat atacttcaaa caaagtttaa agctaaggca
     1741 cttcaagaaa aagtttatat cgaatatgac aaagtaaaag cagatacttg ggatagacgt
     1801 aatatgcgtg ttgaatttaa tcccaataaa ctcacacatg aagaaatgat ttggttaaaa
     1861 caaaatatta tcgactacat ggaagatgac ggttttacaa gattagactt agcttttgat
     1921 tttgaagatg atttgagcga ttactatgca atgactgata aagcagttaa gaaaactgtt
     1981 ttttatggtc gtaatggcaa gccagaaaca aaatattttg gtgtccgtga tagtgataga
     2041 tttattagaa tttataataa aaaacaagaa cgtaaagata acgcagatgt tgaagttgtg
     2101 tctgaacatt tatggcgtgt agaagttgaa ttaaaaagag atatggttga ttactggaat
     2161 gattgtttta atgatttaca catcttgaaa cctgcgtggg ctactttaga aaaaattaat
     2221 gagcaagcta tggtttatac tttgttgcat gaagaaagta tgtggggaaa gctaagtaag
     2281 aatactaaga ctaaatttaa aaaattgatt agagaaatat ctccaattga tttaacggaa
     2341 ttaatgaaat cgactttaaa agcgaacgaa aaacaattgc aaaagcagat tgatttttgg
     2401 caacgtgaat ttaggttttg gaagtaaaat aagttttatt tgataaaaat tgctaattca
     2461 gtataattaa tatttacgag gtgacataac gtatgaaaaa atcagaggat tattcctcct
     2521 aaatataaaa atttaaaatt taggaggaag ttatatatga cttttaatat tattgaatta
     2581 gaaaattggg atagaaaaga atattttgaa cactatttta atcagcaaac tacttatagc
     2641 attactaaag aaattgatat tactttgttt aaagatatga taaaaaagaa aggatatgaa
     2701 atttatccct ctttaattta tgcaattatg gaagttgtaa ataaaaataa agtgtttaga
     2761 acaggaatta atagtgagaa taaattaggt tattgggata agttaaatcc tttgtataca
     2821 gtttttaata agcaaactga aaaatttact aacatttgga ctgaatctga taaaaacttc
     2881 atttcttttt ataataatta taaaaatgac ttgcttgaat ataaagataa agaagaaatg
     2941 tttcctaaaa aaccgatacc tgaaaacacc ataccgattt caatgattcc ttggattgat
     3001 tttagttcat ttaatttaaa tattggtaac aatagcagct ttttattgcc tattattacg
     3061 ataggtaaat tttatagtga gaataataaa atttatatac cagttgctct gcaacttcat
     3121 cattctgtat gtgatggtta ccatgcttca ctatttatga atgaatttca agatataatt
     3181 catagggtag atgattggat ttagttttta gattttgaaa gtgaatttaa ttttatacac
     3241 gtaagtgatc ataaaattta tgaacgtata acaaccacat tttttggttg cttgtggttt
     3301 tgattttgaa tttggttttg aacttatgga ctgatttatt cagtccattt tttgtgcttg
     3361 cacaaaaact agcctcgcag agcacacgca ttaatgactt atgaaacgta gtaaataagt
     3421 ctagtgtgtt atactttact tggaagatgc accgaataaa aaatattgaa gaacaactag
     3481 caaaagattt taaagagtta ttttatttta agtctttata acatgagtga agcgaatttt
     3541 taaatttcga tagaaatttt tacatcaaaa agccccctgt caaaattgac gaagggggtt
     3601 ttttggcgca cgcttttcgt tagaaatata caagattgaa aatcgtgtat aagtgcgccc
     3661 tttgttttga acttagcacg ttacatcaat tttttaaaat gatgtataag tgcgcccttt
     3721 taaattttga gtgattatat tttttgagtt agaaaaaggg attgggaaaa tttcccaaaa
     3781 taatttaaaa aataagcaaa aattttcgat agagaatgtg ctattttttg tcaaaggtgt
     3841 ataccttgac tgtgcttgct gttacattaa gtttattttt aagttattaa aaaagaaata
     3901 gcttttaaag tttggctcgc tgtcgcttta taaagctgat tgacttttga ttgcaaacta
     3961 cttaaagaaa acaaactcgg actattcgtt ttcttctctt tggtttgaac atcagcaatt
     4021 atcccctctt gattgcctat tttagcttgt ttagaagaaa caaaagctaa aagctcctct
     4081 tgggttttaa aacgctgtgt ggggcttaga acgcccttaa acgacccttg gtttactttt
     4141 atactagctt ccacctcgaa aaaaggttct tttttaaaat tctctatggc ttcctggcgc
     4201 tgaaaaataa ggtataaggt gggcgtttga acacgtccta gtgaaaatgt accttgtacg
     4261 cccttctgtt gtaaatttaa cgtatacaaa gggcttgcgt tcatgccgat caaccaatcg
     4321 gcaatttggc gtgtttgcgc ttcttgataa aagggatagt aattcattcc aggttgcaaa
     4381 ttttgaaaac cgcttcggat tacatctttt tctaagctat tgatccatag tcttttaaat
     4441 gttttatctt ttgaaaaggc atttgcttta tggataatcg accaggcgat attttcacct
     4501 tctctgtcgc tatctgttgc aacaataatt gtatttgcct ttttgagaag ttctgcaaca
     4561 attttaaact gctttccctt atcttttgca acttcaaaat cgtatcgatc aggaaaaatc
     4621 ggcaaagatt caagtttcca attttgccac ttttcgtcat aatgacctgg ttctgctaat
     4681 tccactaaat gcccaaaacc aaaggtgata aacgtttcat ctgtaaatag tgggtctttg
     4741 atctcaaaat aaccgtcttt tttggtgctt tgttttaaag cacttgcgta ggctaatgcc
     4801 tggcttggtt tttcagctaa aataaccgta ctcattaact atccctcttt tcattgtttt
     4861 ttctttgatc gactgtcacg ttatatcttg ctcgatacct tctaaacgtt cggcgattga
     4921 ttccagtttg ttcttcaact tctttatcgg ataaaccatt caaaaacaaa tcgaaagcat
     4981 gctttaaacg tggatcattt tctttaaatt tatgctgacg acctttgaat ttgccttttt
     5041 tcttagcaat ttcgattcct tgtgcctgac gttccttaat tttttttcgt tctgattctg
     5101 cttgatactt gtacaattca atgacaaggc tattaatcaa acgccttaaa ttttcatctt
     5161 caataccatt cattgagggt aaatttaaga cttccagggt tgccccctta atttgaattt
     5221 gattcatcaa ttctgttaat tctttattat ttcgtcctaa tcgatctaat tcagtaacaa
     5281 taacaatatc cccttcacga atatagttaa gcatagcttg taattgtggg cgttcgaccg
     5341 attgaccgct taatttgtct gaaaagacct tagaaacgcc ctgtaacgct tgtaattgcc
     5401 gatctaagtt ctgttctttg ctactgacac gtgcataacc aattttagcc attttcaacc
     5461 aacctctaaa attctctcgg ttgcaataac caatcagcaa tatctacttt ttcaatttca
     5521 aattgcttat cagaaattgt cttttcgtaa gcgataaaat cttgcgcata ttgttgctca
     5581 ttaaaaatag ccaccacttc gtcattttct aaaactcgat aaataaattt tttcatttta
     5641 ctcctcctat tatgcccaac ttaaatgacc tattcaccaa gtcaattata ctgctaaaat
     5701 catattagga caaataggta tactctaatg acctataaaa gatagaaaat taaaaaatca
     5761 agtgttcgct tcgctctcac tgcccctcga cgttttagta gcctttccct cacttcgttc
     5821 agtccaagcc aactaaaagt tttcgggcta ctctctcctt ctccccctaa taattaatta
     5881 aaatcttact ctgtatattt ctgctaatca ttcgctaaac agcaaagaaa aaacaaacac
     5941 gtatcataga tataaatgta atggcatagt gcgggtttta ttttcagcct gtatcatagc
     6001 taaacaaatc gagttgtgtg tccgttttag ggcgttctgc tagcttgttt aaagtctctt
     6061 gaatgaatgt atgctctaag tcaaaagaat ttgtcagcgc ctttatatag ctttcttttt
     6121 cttctttttt tactttaatg atcgatagca acaatgattt aacactagca agttgaatgc
     6181 caccatttct tcctggttta atcttaaaga aaatttcctg attcgccttc agtaccttca
     6241 gcaatttatc taatgtccgt tcaggaatgc ctagcacttc tctaatctct tttttggtcg
     6301 tcactaaata aggcttgtat acatcgcttt tttcgctaat ataagccatt aaatcttctt
     6361 tccattctga caaatgaaca cgttgacgtt cgcttctttt tttcttgaat ttaaaccacc
     6421 cttgacggac aaataaatct ttactggtta aatcacttga tacccaagct ttgcaaagaa
     6481 tggtaatgta ttccctatta gccccttgat agttttctga ataggcactt ctaacaattt
     6541 tgattacttc tttttcttct aagggttgat ctaatcgatt attaaactca aacatattat
     6601 attcgcacgt ttcgattgaa tagcctgaac taaagtaggc taaagagagg gtaaacatga
     6661 cgttattacg ccctattaaa cccttttctc ctgaaaattt cgtttcgtgc aataagagat
     6721 taaaccaggg ttcatctact tgttttttgc cttctgtacc gcttaaaacc gttagacttg
     6781 aacgagtaaa gcccttatta tctgtttgtt tgaaagacca atcttgccat tctttgaaag
     6841 aataacggta attaggatca aaaaattcta cattgtccgt tcttggtatg cgagcaatac
     6901 caaaatgatt acacgttaga tcaactggca aagactttcc aaaatattct cggatatttt
     6961 gcgaaattat tttggctgct ttgacagatt taaattctga ttttgaagtc acatagactg
     7021 gcgtttctaa aacaaaatat gcttgataac ctttatcaga tttgataatc atagtaggca
     7081 taaaacctaa atcaatagcg gttgttaaaa tatcgcttgc tgaaatagtt tcttttgccg
     7141 tgtgaatatc aaaatcaata aagaaggtat tgatttgtct taaattgttt tcagaatgtc
     7201 ctttcgtgta tgaacggttt tcgtctgcat acgttccata acgataaacg ttgggtgtcc
     7261 aatgtgtaaa tgtatcttga ttttcttgaa tcgcttcctc ggaagtcaga acaacaccac
     7321 gaccgccaat catgcttgat tttgagcgat acgcaaaaat agcccctttg cttttacctg
     7381 gcttggtagt gattgagcga attttactat ttttaaattt gtactttaac aagccgtcat
     7441 gaagcacagt ttctacaaca aaagggatat tcattcagct gttctccttt cctataaatc
     7501 ctataaaata ggttgtttaa ttaacttggt ttgctttttc attcaactgt ttcaatattg
     7561 catgttttga aaaagatttt tttcctttat aagtcaattt ttttccacta atcgaataaa
     7621 ttattttgtt attttctatt aacttatata tataatcttc cccctccgaa gaaaaatac
//
LOCUS       DAMEET010000267         7279 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.guided.107, whole genome shotgun sequence.
ACCESSION   DAMEET010000267 DAMEET010000000
VERSION     DAMEET010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.guided.107"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..541)
                     /locus_tag="PB780_002787"
     CDS             complement(<1..541)
                     /locus_tag="PB780_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDE0734750.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGV"
     gene            810..1706
                     /locus_tag="PB780_002788"
     CDS             810..1706
                     /locus_tag="PB780_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HDE0734751.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1856..2797)
                     /locus_tag="PB780_002789"
     CDS             complement(1856..2797)
                     /locus_tag="PB780_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDE0734752.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2923..4044
                     /locus_tag="PB780_002790"
     CDS             2923..4044
                     /locus_tag="PB780_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDE0734753.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            4302..5804
                     /gene="eat(A)"
                     /locus_tag="PB780_002791"
     CDS             4302..5804
                     /gene="eat(A)"
                     /locus_tag="PB780_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HDE0734754.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5832..6398
                     /locus_tag="PB780_002792"
     CDS             5832..6398
                     /locus_tag="PB780_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDE0734755.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            6609..>7279
                     /locus_tag="PB780_002793"
     CDS             6609..>7279
                     /locus_tag="PB780_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDE0734756.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLS"
BASE COUNT         2424 a         1303 c         1497 g         2055 t
ORIGIN      
        1 aaacgcctgt gcccaacatt tgctgaccga caagataagt agcaaaaatg atcagtaaga
       61 taatgacact gattttagca aaagccagcc aatattctgt ttctgcgaat aaccgtaccg
      121 aataaagatt gatgatcgta gttaaaacag ccaacataaa aacaaatacc caggctggaa
      181 tgtgcgggaa ccacaactgc aaaaaggaag cagctgcaac agcttcagcg atgatattga
      241 taatccacat agaccagtag acccaatcag taaaatcagc agaatgagca cctagataag
      301 ggcgtatcaa accagataat ccgtgatgcg tgtcgccacc gcttaaaacg atttttccca
      361 gacctttcat cacgatatat aaaatcagcc caccaatcaa aaaagcaatc aatacagaag
      421 ggccggcgat cccaatcgct tcgccacttc ctttaaatag acctgcaccg attgctccac
      481 ctaaagctaa catggtgata tgtctggagc taagttgttt tttcaatttt ttatcttcca
      541 taaatgacct ctttctttta aattcacaat caaaatttat attaagaata aatagcaata
      601 tttaacaatg actaatgata acaaattttc agacaattac aactattcag aaaactttta
      661 ggcgaatttt aatagaagaa tgatttattt catcatattt tcatacaaat gttaaatttt
      721 catacaaata taggaaacgt tttactggct ttttactatt taaacaggct atcataggaa
      781 aaagtgacag agaaagtgag gcggaaaaga tgaacaaaaa tgacgtactg ctagttgcaa
      841 aaaataaatc actttatttt ttgccttcca tccgctcttt agatgtttgg tccggaaaag
      901 atttactaca gtcggaattc attaaatcag ctagacaaca gttggcaaat tatcagctga
      961 tcgttttttt agattatgga tttgatatgg agatggctcg acgcgtgcgc ccatttacca
     1021 atgcaaaaat tgtgttattt ttttggaatc acttcaaagc agaacacgag gcgatgctga
     1081 agcaggcaca agatgaaccg gcaatcgatg agatttatca ttttgatttt ttggaagcaa
     1141 aagaacttgg gctgaaacat aatagttcat tttattcgaa acatatgaat ttttcttcta
     1201 gcgaaccaaa gacggatcta ttttttggtg caacgaataa tgggagaaaa gaacgagcag
     1261 aaagctataa gcaggaattt ataaaaagag atgttacgac caactatttc attcttccac
     1321 atcgagggaa tgaacaacct ggatatctta cttatgaaga gtacctgaaa aaaacagggg
     1381 aatcgcgcgg gattctggaa ctgttgcggg agggtcagca aggagtgact ttgcggacat
     1441 ttgaatccat gtattttcaa aagaaactag tgacggataa tcaagcgatt gctcattatc
     1501 atttttatcg tcctgaaaat atttttttgc ttcgagaacg tgatttagat gaattaccgg
     1561 aatttctgca tacaccattt aagccaatcc tatcagagct attggaattt tatgatgcag
     1621 aaaattgggc aaaacgattt atcactaacg attccactat ttatgagcaa tacgaatatg
     1681 acctctcctc tgttgaaaac aaataaaaaa cctacatttt gaagagatta tgtactgaaa
     1741 caaacaagca caattcttat ggcatcatga gagttgtgtt ttctttttga tcttttattg
     1801 ggcttaaaaa aagagaacca gctgctgatt cagctgattc tcatcagaga gccttttatt
     1861 ctgcgccaag gattccttct tttgcatgga ctaattgggt caaaaaggca caatatggtg
     1921 tagcaatatc atatttttgt ccttttcgcc aaatggcacc attgatgtag tcgatttcag
     1981 tcaaacgatg gtttttgatc aggtcttggt acatagatgg gtaatgcaac ccgatgttgt
     2041 caggatcata agtagaagca atatgttggt acacttcttc ttgatctagt atgattcctt
     2101 cttttgctgc gatagaagca aattcagata cgattgttcg aaccatgctc tctgccgctt
     2161 tttgcgcgcc tagttcagca atattacaat ccaaaatcgt gcataaacca ttcaatgttc
     2221 catttacaca agcttttcgc caaatggaat aacgaacgtt atggctatag atcggattta
     2281 aatttgcttc ttgaaagact tctacaactt tttttgtaaa tgcttcgcct tctggttcca
     2341 agttctctag ttcgatttct ccatcaccta acaatgtgac ttttccggga ccagctagac
     2401 cagctgtcca catcgtgatc ccaaataaga tatttttctt tggcacatat ttttccagta
     2461 catcttcatg acctaatcca tttagtaaac acaaaacata agtattgtca gtaataatgg
     2521 attggatcga acaaaacata tcatctaatt gattggcttt tgttaatgct acgatcaagt
     2581 ccacgtgttc gttgctttcg atgatttctt ctggtgaata gattggcagt tttgcgacca
     2641 cttctttacc gttaaagtca gcaatcaacc catttgtacg aatggcttca atgtgagcag
     2701 gccaacgatc gatcaagagg acctcatttc cgctttgatg tagcatcaat ccgattcggc
     2761 tgcccattgc tccggcccca gctattgcga ttttcatgtt ttttctctcc tttattcaat
     2821 gagttccctt ttagtatata gaaaaatgga agaaaatagc attgttttgt ttgaaaaaaa
     2881 tgaaaaaaag tgtcttactt aagaagaaag tgagggatac agatggatat tttggcggca
     2941 attgattcat ttaaaggaag tgcttcttca tttgaactaa atcaggcagt tctaaatggc
     3001 tgggaagatc gtaaaggaag aaaaatcaat gtgccgattg cagatggcgg agaaaatact
     3061 gcagaagcaa tctattacgc gctcagggga acatggcgta ccattgatgg gatggatctc
     3121 ttattccgtc agcgaaaaat cagctattta ttgacaactt ggcaaggaaa aaacaccgca
     3181 gtcatcgaat ctgcacaagc attggggtta gatctacttg ataaaccaac ggatcaaact
     3241 atccgacttg cttccagtta tgccttagga gaacttatta aagatgcact gcttcaagaa
     3301 gtagaacaaa ttattgtcac tttaggagga agcgggtgta gcgatggtgg tctaggatta
     3361 ctccaatcct tgggtgcttc tttagaaggg tacacagaag gaaatccttt attatctact
     3421 aaaaaacttc attttgatcg aagaaacgtt cttgtttcta aagtaaaatt gattatcgct
     3481 gcagatgtta ccaatccata tacaggtcaa caaggagctg cttggcaatt tggaagacaa
     3541 aaaggaggaa tagatacgac gctagctttc ttagataaac aagcaggaaa gattgctcag
     3601 caaataaaag atttctggga ggtggattta gatgatattc caggaagtgg agcagcaggc
     3661 ggtattggtg gagccctttt tttgttggga gcgaagatgg tttctggttt cgatttagtc
     3721 agccaaatca ctggattgga aaaaaagatc gaacaagcag atgtcatcat tacaggagaa
     3781 gggcggatcg atcagcaaac gatccacgga aaagttccat acggtgtagc agtgcttgca
     3841 aaaaaatatc gaaaaccaat aatcgctatt tgcggaagta gagaccaaga gctagaagga
     3901 ctttctgatt ttctcccaat tgtcttaagt attcatttgg gtccaatagg tttagaagaa
     3961 gcaatggaac acaggcaaac attagaaaaa acaaaaatct taggacagac acttagcaga
     4021 tttttcacca tcctcccgaa gtagttgaca aaatcatttt ctttatgcta tcgtaatttc
     4081 aaatatagga caaagtgagg gatccgtgca ttgaaaaatt aagtcgattg atgaaagctg
     4141 cattcagact gtaccttgtg gagacaagga agcagtgtgc agattttcgg gattggcttg
     4201 gttttttcga tgtatgggtc tttttgtgtg tatccaaccc ttgcatcctc tttagccagt
     4261 tctgaaaata ggactggtct ttttttacga ggtgatggaa aatgtctaaa atcgaaataa
     4321 aaaatctgac attcggctac gacagccaag gcacattatt atttgaacaa gcaaatctaa
     4381 attttgacac acaatggaaa ctaggactta tcggacgaaa cggtcgagga aagacaactt
     4441 tactgaatat tctacaaaac aaactacctt atcaagggca agtaatccat cagcaagaat
     4501 ttgcctattt cccgcaacag acaaaagata aagaacgttt aacctattac gtgttaaatg
     4561 atattacgga ttttgagata tgggaaatcg aaagagagct ccaattgatg caaacagatc
     4621 ctgaaatctt atggagagaa ttcagcacac tatcgggggg agagaagaca aaagtcctac
     4681 tggcactttt atttgtggat gacactcatt tcccgttaat cgatgaacca acgaatcatt
     4741 tggatatctc tggtagaaaa caagtagcgg cttatttgaa aaagaaaaaa caaggcttca
     4801 tcgtggtcag ccatgaccgg ggatttatcg atgaagtagt ggaccatgtt ttagcaatcg
     4861 aaaaaagtca actggaactt tatcaaggga atttctctat ctatgaagaa cagaaaaaac
     4921 ttcgtgatga atttgaaatg gctcaaaatg aaaaattgaa aaaagaagtc agtaggctaa
     4981 agaaaacagc agctgaaaaa gccgaatggt ctcgttcccg agaaggagat aaaacaaaga
     5041 aacaagtcgg attcatcgat actgaatcta gacgagtgaa taaaggagca gtgggtgctg
     5101 atgctgcacg gacgatgaaa cgatccaaag caatcgtgaa tcggatggag acccagatca
     5161 gcgagaaaga aaaactatta aaagatatcg aatatatcga ttctttgacg atgaatagcc
     5221 aagcgtctca ccataagcga cttttaagcg tagaagatct tcaattaggg tatgaaaatc
     5281 tgttattcga gccaattcat tttacaatcg agcctcatca gcgggtggcg atttcaggtc
     5341 ctaacggtgc aggaaagtca tccattatcc attatcttct gggggcattc aacggcaagg
     5401 ttataggaga aaaataccag ccaaaacatc tgagcattag ttatgcaagc caaaattatg
     5461 aagacaatcg aggaacgttg gcggaatttg cagagaaaaa ccaagtagac taccaagcat
     5521 ttttgaacaa cctccgaaag cttgggatgg aaagagatgt ttttcataac aagatcgagc
     5581 agatgagtat gggccaacgg aaaaaagtgg aattggctaa atctttatca cagccagctg
     5641 aactatatac atgggatgaa ccattgaatt atttggatgt cttcaatcaa gaacaattag
     5701 aacaactgat cttgaacgtg aaacctgcca tgttactagt ggaacatgat caaaccttcc
     5761 tggataaagt atctactgag attatttctc ttgagagaat ctaacccttg taatctcaag
     5821 aacaacaagg tatggttaag gggaactttg ccaacgaagg agagaatcac atgctttatg
     5881 taacgagaca cggagaaaca acttggaacg cacaaggatt agtttgcgga cgggcagatg
     5941 tgccgctgac tgaaaaagga cagatgcagg cacagaaact agcagaaaag gtagtagatt
     6001 tgcctgttcc aatcacgaaa atcatccatt cccctcttca acgagcaaga gatacagcac
     6061 aagcagttgc tgatcgtctc agcttgccgc tgaccgttga tgaacgattg gtagaaatgg
     6121 attttgggga ttatgatgga ctgccaagca aagatgagaa ctttcaaaaa gcacgtttag
     6181 cgttcgctgt acgattccca aatggggagt cagtcttaga tgtgtatgca cggatcgttc
     6241 ctttattgaa agaatgcatc gaagacgaag aaaatgtcta tctgttagtt tgccataatg
     6301 ccttgataag agtgatcaat gcttattttc atccgatgcc taatgagggg ttctttacat
     6361 tcatggtaga taatacagaa ttggtttctt atgagtaatt atgtttttgt gttatttaaa
     6421 taaaatattt caaggcactg cttttgctta gcagtgcctt ttttaggtta tgacaattct
     6481 tttgataata taaactcaac ctttagaaat gaaatttaat tattttaata aaagtattga
     6541 aattttgttt ctttagatat atactctagt catagaaatg aagacggata cagaaaaaga
     6601 gggataatat gtttggcttt tcaatattta tgaacgattc tttgtcagaa gagaagaaac
     6661 agtatatccg ccaaatggca tccaatggct ttgtcggtat tttcacttcc atgcatatac
     6721 cagaagatga tgtttcggca tatcaaaagc gattgatcga actaggaagc gaagcgaaaa
     6781 agaataaact agaattgatg gtcgatattt caggagaggc attggaacga gcgggttttt
     6841 cattagaaga tctaacgcct ttgaaagcaa ttggtgtaac aggtttaaga atggattatc
     6901 atatttccaa tcagcagatt gccgactggt cacatcaatt gaaaatcagt ttgaatgcta
     6961 gcacgatcac gcctaaagat atcgatgaat tgaaagaagc agaagctgat ttctcacaga
     7021 ttgaagcttg gcataattat tatccacgtc ccgaaacagg gctggacaaa gaatggtatc
     7081 agaaaaaaaa tcaatggctc aaaattcaag gtctccttgt ccaaggtttt gtaccaggag
     7141 atacagagtt gagaggccca ctatatcaag gattgccaac tttggaagaa catcgaggga
     7201 tccatcctct tgcaggcgct ctagatctat tagcaagtaa cacggatatt gtttacatcg
     7261 gagatgcagg tctgtcaga
//
LOCUS       DAMEET010000268         5586 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.guided.122, whole genome shotgun sequence.
ACCESSION   DAMEET010000268 DAMEET010000000
VERSION     DAMEET010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.guided.122"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..212
                     /locus_tag="PB780_002794"
     CDS             <1..212
                     /locus_tag="PB780_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDE0734757.1"
                     /translation="ELEQEESIRFQNCSLFPLYHGSAKSNIGIDNLIEVITNKFYSST
                     HRGPSELCGNVFKIEYTKKRQRLAY"
     gene            447..683
                     /locus_tag="PB780_002795"
     CDS             447..683
                     /locus_tag="PB780_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDE0734758.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            690..2642
                     /locus_tag="PB780_002796"
     CDS             690..2642
                     /locus_tag="PB780_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HDE0734759.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2714..3211)
                     /gene="dfrG"
                     /locus_tag="PB780_002797"
     CDS             complement(2714..3211)
                     /gene="dfrG"
                     /locus_tag="PB780_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HDE0734760.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3573..4613
                     /locus_tag="PB780_002798"
     CDS             3573..4613
                     /locus_tag="PB780_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDE0734761.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            4711..4899
                     /locus_tag="PB780_002799"
     CDS             4711..4899
                     /locus_tag="PB780_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HDE0734762.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(4957..5310)
                     /locus_tag="PB780_002800"
     CDS             complement(4957..5310)
                     /locus_tag="PB780_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDE0734763.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
BASE COUNT         1930 a          905 c          980 g         1771 t
ORIGIN      
        1 tggaactcga acaagaggaa agcataagat ttcagaattg ttctctgttc cctctttatc
       61 atggaagtgc aaaaagtaat atagggattg ataaccttat agaagttatt actaataaat
      121 tttattcatc aacacatcga ggtccgtctg aactttgcgg aaatgttttc aaaattgaat
      181 atacaaaaaa aagacaacgt cttgcatatt aagtagttca gttttggagt acaaaaagtg
      241 aaaatgtaaa cctaaatgag tatcaaattg caactgaatt tgatactcgt tttttattgt
      301 taaatcaacg tttgttgtta atttacacca caaaatgtaa gttaaaatgt atataaaaat
      361 gcgaacgtga aataattatt gtaggaatgc aaattggtgt gttaaaatgg ttacaattta
      421 ctaagcaaaa aggggtgcat tttcctatgc aatttcttga gccaaaaaat aagaacgcta
      481 agtctgtgga ttgggaaata tcagagcaag taagggtgat agttaagcaa tacgctgaat
      541 atgcagagcg tactgaaagc gaagcagtgg atgaattctt attgaacatt ttagacgata
      601 agaagtttat tgaatggatt gccaacaagc gaagtaataa gcggattgtt gaaaaaatgg
      661 gtatcaaaga tagagtgggt tgatagacaa tgaccaagct taaaagatta gccacgaagg
      721 aagatgaaat tgtagatgtt aaaattccga tttcaaacga agaattaaaa gatagggcta
      781 aacaatatga tttattaact ccaaaacgat ttgcaacaag gtataacaaa atgttgttct
      841 tgccaacgtc ttttaaatgg aacggttctg aatatcccat tcaatataat tactgcatca
      901 atccgttttg ttgtaatttt gggaaagaac agcacaaatt caaagatgta aaagggaaac
      961 caagtcgata taaaatgact ggtagttcca aagataaagg tcataaaggt atgtactgca
     1021 atgacaatcc tattggcaga ggtgtatcgc aaaactgtac agtaacccct ttatctaatt
     1081 ggtctgttgt tgaagaaatt aaacgcctta tagaaatcaa tagcatccaa gatgttgagc
     1141 cagattatca atttcataaa gaaggctgct cggaagaaga atcaacccca ttcaatgagc
     1201 cgaagcaatt ttacaaaagg gggaaaagta ggggcaaatc tcaacgctat caatgtaagg
     1261 cgtgtaagaa gttcactaat gttctaccaa aaagagaaga aaccactact tatcaccaac
     1321 aaaagaacac cattcttcca atgttcgcta agatggttgt tggaagggtt tcagtcagtc
     1381 gtacttgtga tattttagga attggagttg gaacctacta tcataagctg gagtggctgt
     1441 ataggcgttg tttggagttt ttagaacgat atgagacaca accccttcaa acaaagaaat
     1501 ttaacgagat gtggcttaat acggacaaga tgcactacta cctaaataac gtccgtaaga
     1561 aaggacaagg ttcaaagaaa tatacggggt ttgaagacct taatatgcaa acgtatatag
     1621 ttgtatctgc tgaggtctta tcaagatatg tgttccgttc tgatgttgcc tatgattgga
     1681 atatttcaat ggatgaatta aatgaagata caagaaaatt caaagaagac catctcaata
     1741 cttttagtcg taaaaatgat aggttagatt ggtcttatta tccacaagaa ccatccgcta
     1801 acgatagcga aaataggaat gcttatcttc acgaattagg gaaaattact aataggagta
     1861 gatttgtcga tggtttaaat gttgatgctc cttatactac aactgctcac tattggttaa
     1921 ttaaacaaat ggttaatgcc gacgaatggc gaatgattag tgatgatgac ttctctatta
     1981 gaaacgcctt ttatagagta ttcacgaaag aattaaggtt gagcgatgca catcatttta
     2041 tttgccaagt taacaagacg aaaagcagaa aacaatgctt aaaagaattc ggacaagcaa
     2101 aagcagaact gctcgattgg ggagatatca gaggattcaa aacaaagttt ctacggactt
     2161 tggcatctca ttatctaact gaattactaa caagtcatca attccacgaa gaggctatta
     2221 gtaaagatgg agaaagatat cgcaaatatg ccgataaccc tatcaaacat ccattagcca
     2281 ctaaggataa aggattttat tcagttgatt gtagaacgga tttatctgct ctggaaccaa
     2341 acgaaatcgc aaagatgcta ttaaatgtta atgaccattc gaccaatagc tttattcaac
     2401 agataagacg ttatatatca tctcttgaaa gaccattaac aacagctcgt ggtgataaga
     2461 agagctatat ttacgctaac ttcaatccga agtatgctca atttgcgatt acaattctaa
     2521 gaacttacta caacttttgt cgccctttta aatcagcgga taaaaaggtt ttgacacctg
     2581 ctcaaagatt agggattaca gataaacaat ttgattggaa agatataatt tatttcaaat
     2641 gacaaaaaga gcttggagta atcccaagct cttgtgcttt atctttactg ttcttcttca
     2701 attaacgcac ccgttaactc aataagtttt ttctttcata tacatgaaaa tagtagttat
     2761 acggattttt atcattcttt atcccttttt gggcaaatac ctcattccat tcctcataat
     2821 tcacttctgg aaaaaaagta tctccttcga attcatgatg tatttttgtg atgtacattt
     2881 tctcaacata agggaaaaac aaattataaa tctgttctcc tccgaaaata aaaatttctt
     2941 cttcgttttt acataactca aaaacatctt ctattgaatg aacaatttca caaccattaa
     3001 aggtaaaccc cttatctctc gtcagaataa tatttcttct gtcaggtaag gctcttccga
     3061 ttgattcaag gttcttccta cctaatatta tcggatgtcc ctttgtagta tttttaacat
     3121 attcccagtc cttgggaatc ctccaaggaa tgtcattctc tttgccaatc actctattct
     3181 tatccatcgc agcaatcaaa gaaactttca tttataacac agccttttta actaatttac
     3241 catctaattt cttatttcta tcaaaaaacc ttttctcatt atcctgcccg ttagttgaat
     3301 aagcaatttt ttctataagt tatattatca taataccaca aaatcacaaa ttttcattca
     3361 attatcattt tcaattgcat tttataaatc attttgtatg taatttggta tgtaagttaa
     3421 aatgaaaaaa gtagtccatt ttgaagtaca ttttgtcatt ttgtactcca aaactgaact
     3481 acttacttgc atatatacgc ctttatagtg gagtactaca tttacgagat tcggttagag
     3541 tatcagaaaa agaaaaaata aaagttacag aaatgtatac ttcaataaat ggtgaattat
     3601 gtaagattga tagagcttat tctggagaaa ttgttatttt gcaaaatgag tttttgaagt
     3661 taaatagtgt tcttggagat acaaaactat tgccacagag aaaaaagatt gaaaatccgc
     3721 accctctact acaaacaact gttgaaccga gtaaacctga acagagagaa atgttgcttg
     3781 atgccctttt ggaaatctca gatagtgatc cgcttctacg atattacgtg gattctacga
     3841 cacatgaaat tatactttct ttcttaggga aagtacaaat ggaagtgatt agtgcactgt
     3901 tgcaagaaaa gtatcatgtg gagatagaac taaaagagcc tacagtcatt tatatggaga
     3961 gaccgttaaa aaatgcagaa tataccattc acatcgaagt gccgccaaat cctttctggg
     4021 cttccattgg tttatctgta tcaccgcttc cgttgggaag tggaatgcag tatgagagct
     4081 cggtttctct tggatactta aatcaatcat ttcaaaatgc agttatggaa gggatacgct
     4141 atggttgcga acaaggatta tatggttgga atgtgacgga ttgtaaaatc tgttttaagt
     4201 acggtttata ctatagccct gttagtactc cagcagattt tcggatgctt actcctattg
     4261 tactggagca agcctttaga aaagctggaa cagaattgtt agagccatat cttagtttta
     4321 aagtttatgc accacaggaa tatctttcac gggcatataa cgatgctccc aaatattgtg
     4381 caaatatcgt aaatactcaa ctgaaaaata atgaggtcat tattattgga gaaattcctg
     4441 ctcgatgtat tcaagattat cgcaatgatt taactttttt tacaaatggg cttagtgttt
     4501 gtttagcaga gctaaaagga tatcaggtta ccactggcga acctgtttgc cagacccgtc
     4561 gtctaaatag tcggatagat aaagtaagat atatgttcaa taaaataact tagtgcgttt
     4621 tatgttgtta tataaatatg gtttcttatt aaataagatg aaatattctt taatatagat
     4681 ttgaattaaa gtggaaagga ggagattgtt attataaact acaagtggat attgtgtcct
     4741 atttgtggaa ataaaacaag actacgaata cgagtggata ctatacttaa aaatttccct
     4801 ttatactgcc ccaaatgtaa gaacgaaact ttaattaatg ttcaaaaaat gaatataata
     4861 acaatcaaag agccagacgc caagacgcag agccgataat ttgagaaatg aaactctcat
     4921 cttatcggct ctttttgttt atctgaattt tactgactag ccttcaatat ttcttgcttc
     4981 attgatacct tttgctagtg cttccattaa ggatagttct ttgtctgtaa agctatccat
     5041 gtatttttct acctgtaacc ttcgagtgct tttaatcaaa ttatctgtag gtaagaaaaa
     5101 ttcatcaata gatacatgga gtaatgatac aagatcatac agcacttgtg tacttgggtg
     5161 ttgtcctttg ttttctatat ttgttaaata acgtgggtca atttcaatca ttgttcccac
     5221 ttgttcacga gtgagacccc gtttcattcg agcctcttta atcgctaaac caacggctct
     5281 aaaatcgtat ttatcttctt ttttacgcat actaaatcac ctctatacat tttactgttc
     5341 ctatataacc ataaacaggt atagaaaaac atgttatata gtttatgagt tcctatagaa
     5401 cactgtggaa tttattaatg cgatgaatcc aatttcaaat agaaaggtcg ctgattttat
     5461 ttacctatac aactttataa aatttagtca tactcctaga aagtagataa aattggcgac
     5521 ttaatggagg gaaaagtcgc catgagaaag ataatctgtc atctagttct cttaattaag
     5581 ttctta
//
LOCUS       DAMEET010000269         4802 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.guided.130, whole genome shotgun sequence.
ACCESSION   DAMEET010000269 DAMEET010000000
VERSION     DAMEET010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.guided.130"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            91..786
                     /gene="vanR"
                     /locus_tag="PB780_002801"
     CDS             91..786
                     /gene="vanR"
                     /locus_tag="PB780_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDE0734764.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            764..1918
                     /locus_tag="PB780_002802"
     CDS             764..1918
                     /locus_tag="PB780_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HDE0734765.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2133..3101
                     /locus_tag="PB780_002803"
     CDS             2133..3101
                     /locus_tag="PB780_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HDE0734766.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3094..4125
                     /gene="vanA"
                     /locus_tag="PB780_002804"
     CDS             3094..4125
                     /gene="vanA"
                     /locus_tag="PB780_002804"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HDE0734767.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4131..4739
                     /locus_tag="PB780_002805"
     CDS             4131..4739
                     /locus_tag="PB780_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HDE0734768.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1482 a          921 c         1119 g         1280 t
ORIGIN      
        1 atggcctttt atcttttaca caattatatg gactttatcc tataatctga taaattatta
       61 agggcaaact tatgtgaaag ggtgataact atgagcgata aaatacttat tgtggatgat
      121 gaacatgaaa ttgccgattt ggttgaatta tacttaaaaa acgagaatta tacggttttc
      181 aaatactata ccgccaaaga agcattggaa tgtatagaca agtctgagat tgaccttgcc
      241 atattggaca tcatgcttcc cggcacaagc ggccttacta tctgtcaaaa aataagggac
      301 aagcacacct atccgattat catgctgacc gggaaagata cagaggtaga taaaattaca
      361 gggttaacaa tcggcgcgga tgattatata acgaagccct ttcgcccact ggagttaatt
      421 gctcgggtaa aggcccagtt gcgccgatac aaaaaattca gtggagtaaa ggagcagaac
      481 gaaaatgtta tcgtccactc cggccttgtc attaatgtta acacccatga gtgttatctg
      541 aacgagaagc agttatccct tactcccacc gagttttcaa tactgcgaat cctctgtgaa
      601 aacaagggga atgtggttag ctccgagctg ctatttcatg agatatgggg cgacgaatat
      661 ttcagcaaga gcaacaacac catcaccgtg catatccggc atttgcgcga aaaaatgaac
      721 gacaccattg ataatccgaa atatataaaa acggtatggg gggttggtta taaaattgaa
      781 aaataaaaaa aacgactatt ccaaactaga acgaaaactt tacatgtata tcgttgcaat
      841 tgttgtggta gcaattgtat tcgtgttgta tattcgttca atgatccgag ggaaacttgg
      901 ggattggatc ttaagtattt tggaaaacaa atatgactta aatcacctgg acgcgatgaa
      961 attatatcaa tattccatac ggaacaatat agatatcttt atttatgtgg cgattgtcat
     1021 tagtattctt attctatgtc gcgtcatgct ttcaaaattc gcaaaatact ttgacgagat
     1081 aaataccggc attgatgtac ttattcagaa cgaagataaa caaattgagc tttctgcgga
     1141 aatggatgtt atggaacaaa agctcaacac attaaaacgg actctggaaa agcgagagca
     1201 ggatgcaaag ctggccgaac aaagaaaaaa tgacgttgtt atgtacttgg cgcacgatat
     1261 taaaacgccc cttacatcca ttatcggtta tttgagcctg cttgacgagg ctccagacat
     1321 gccggtagat caaaaggcaa agtatgtgca tatcacgttg gacaaagcgt atcgactcga
     1381 acagctaatc gacgagtttt ttgagattac acggtataac ctacaaacga taacgctaac
     1441 aaaaacgcac atagacctat actatatgct ggtgcagatg accgatgaat tttatcctca
     1501 gctttccgca catggaaaac aggcggttat tcacgccccc gaggatctga ccgtgtccgg
     1561 cgaccctgat aaactcgcga gagtctttaa caacattttg aaaaacgccg ctgcatacag
     1621 tgaggataac agcatcattg acattaccgc gggcctctcc ggggatgtgg tgtcaatcga
     1681 attcaagaac actggaagca tcccaaaaga taagctagct gccatatttg aaaagttcta
     1741 taggctggac aatgctcgtt cttccgatac gggtggcgcg ggacttggat tggcgattgc
     1801 aaaagaaatt attgttcagc atggagggca gatttacgcg gaaagcaatg ataactatac
     1861 gacgtttagg gtagagcttc cagcgatgcc agacttggtt gataaaagga ggtcctaaga
     1921 gatgtatata attttttagg aaaatctcaa ggttatcttt actttttctt aggaaattaa
     1981 caatttaata ttaagaaacg gctcgttctt acacggtaga cttaataccg taagaacgag
     2041 ccgttttcgt tcttcagaga aagatttgac aagattacca ttggcatccc cgttttattt
     2101 ggtgcctttc acagaaaggg ttggtcttaa ttatgaataa catcggcatt actgtttatg
     2161 gatgtgagca ggatgaggca gatgcattcc atgctctttc gcctcgcttt ggcgttatgg
     2221 caacgataat taacgccaac gtgtcggaat ccaacgccaa atccgcgcct ttcaatcaat
     2281 gtatcagtgt gggacataaa tcagagattt ccgcctctat tcttcttgcg ctgaagagag
     2341 ccggtgtgaa atatatttct acccgaagca tcggctgcaa tcatatagat acaactgctg
     2401 ctaagagaat gggcatcact gtcgacaatg tggcgtactc gccggatagc gttgccgatt
     2461 atactatgat gctaattctt atggcagtac gcaacgtaaa atcgattgtg cgctctgtgg
     2521 aaaaacatga tttcaggttg gacagcgacc gtggcaaggt actcagcgac atgacagttg
     2581 gtgtggtggg aacgggccag ataggcaaag cggttattga gcggctgcga ggatttggat
     2641 gtaaagtgtt ggcttatagt cgcagccgaa gtatagaggt aaactatgta ccgtttgatg
     2701 agttgctgca aaatagcgat atcgttacgc ttcatgtgcc gctcaatacg gatacgcact
     2761 atattatcag ccacgaacaa atacagagaa tgaagcaagg agcatttctt atcaatactg
     2821 ggcgcggtcc acttgtagat acctatgagt tggttaaagc attagaaaac gggaaactgg
     2881 gcggtgccgc attggatgta ttggaaggag aggaagagtt tttctactct gattgcaccc
     2941 aaaaaccaat tgataatcaa tttttactta aacttcaaag aatgcctaac gtgataatca
     3001 caccgcatac ggcctattat accgagcaag cgttgcgtga taccgttgaa aaaaccatta
     3061 aaaactgttt ggattttgaa aggagacagg agcatgaata gaataaaagt tgcaatactg
     3121 tttgggggtt gctcagagga gcatgacgta tcggtaaaat ctgcaataga gatagccgct
     3181 aacattaata aagaaaaata cgagccgtta tacattggaa ttacgaaatc tggtgtatgg
     3241 aaaatgtgcg aaaaaccttg cgcggaatgg gaaaacgaca attgctattc agctgtactc
     3301 tcgccggata aaaaaatgca cggattactt gttaaaaaga accatgaata tgaaatcaac
     3361 catgttgatg tagcattttc agctttgcat ggcaagtcag gtgaagatgg atccatacaa
     3421 ggtctgtttg aattgtccgg tatccctttt gtaggctgcg atattcaaag ctcagcaatt
     3481 tgtatggaca aatcgttgac atacatcgtt gcgaaaaatg ctgggatagc tactcccgcc
     3541 ttttgggtta ttaataaaga tgataggccg gtggcagcta cgtttaccta tcctgttttt
     3601 gttaagccgg cgcgttcagg ctcatccttc ggtgtgaaaa aagtcaatag cgcggacgaa
     3661 ttggactacg caattgaatc ggcaagacaa tatgacagca aaatcttaat tgagcaggct
     3721 gtttcgggct gtgaggtcgg ttgtgcggta ttgggaaaca gtgccgcgtt agttgttggc
     3781 gaggtggacc aaatcaggct gcagtacgga atctttcgta ttcatcagga agtcgagccg
     3841 gaaaaaggct ctgaaaacgc agttataacc gttcccgcag acctttcagc agaggagcga
     3901 ggacggatac aggaaacggc aaaaaaaata tataaagcgc tcggctgtag aggtctagcc
     3961 cgtgtggata tgtttttaca agataacggc cgcattgtac tgaacgaagt caatactctg
     4021 cccggtttca cgtcatacag tcgttatccc cgtatgatgg ccgctgcagg tattgcactt
     4081 cccgaactga ttgaccgctt gatcgtatta gcgttaaagg ggtgataagc atggaaatag
     4141 gatttacttt tttagatgaa atagtacacg gtgttcgttg ggacgctaaa tatgccactt
     4201 gggataattt caccggaaaa ccggttgacg gttatgaagt aaatcgcatt gtagggacat
     4261 acgagttggc tgaatcgctt ttgaaggcaa aagaactggc tgctacccaa gggtacggat
     4321 tgcttctatg ggacggttac cgtcctaagc gtgctgtaaa ctgttttatg caatgggctg
     4381 cacagccgga aaataacctg acaaaggaaa gttattatcc caatattgac cgaactgaga
     4441 tgatttcaaa aggatacgtg gcttcaaaat caagccatag ccgcggcagt gccattgatc
     4501 ttacgcttta tcgattagac acgggtgagc ttgtaccaat ggggagccga tttgatttta
     4561 tggatgaacg ctctcatcat gcggcaaatg gaatatcatg caatgaagcg caaaatcgca
     4621 gacgtttgcg ctccatcatg gaaaacagtg ggtttgaagc atatagcctc gaatggtggc
     4681 actatgtatt aagagacgaa ccatacccca atagctattt tgatttcccc gttaaataaa
     4741 cttttaaccg ttgcacggac aaacggttct gttgcaaagt tttaaataaa gaataaaatc
     4801 cc
//
LOCUS       DAMEET010000270         4275 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.guided.139, whole genome shotgun sequence.
ACCESSION   DAMEET010000270 DAMEET010000000
VERSION     DAMEET010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.guided.139"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            284..748
                     /locus_tag="PB780_002806"
     CDS             284..748
                     /locus_tag="PB780_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDE0734769.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            732..1361
                     /locus_tag="PB780_002807"
     CDS             732..1361
                     /locus_tag="PB780_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDE0734770.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            1715..3193
                     /gene="msr(C)"
                     /locus_tag="PB780_002808"
     CDS             1715..3193
                     /gene="msr(C)"
                     /locus_tag="PB780_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDE0734771.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3240..3686
                     /locus_tag="PB780_002809"
     CDS             3240..3686
                     /locus_tag="PB780_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDE0734772.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3885..>4275)
                     /locus_tag="PB780_002810"
     CDS             complement(3885..>4275)
                     /locus_tag="PB780_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDE0734773.1"
                     /translation="KLDQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPT
                     QKDEFLAKIYAFSTLDKTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVV
                     IQRRIMLCQQELQWCQQVREQMQQFFE"
BASE COUNT         1383 a          714 c          883 g         1295 t
ORIGIN      
        1 gggattcttg caaaaggcga tttcggaaat ttttggcggt gtaaaatttt ctactgcaga
       61 agagctatct tctatagttt gataagaatc aataatcttg gttttagcgt tgtgctttct
      121 ttatcatttt ttccgttcat tctacaaaaa atttcttttc tctatacttt tctattgaga
      181 gcgttttttt tattacacag ctgattcttt gacttttttc tttttttatt ctattattga
      241 caagtcaatc gttgacgagt caattattag aaaggaggaa attatgtctt tagaagagat
      301 tagtcacctt ttatatcaaa tcaaattagc cgatcaaaaa atcgtccaga tctttgaaga
      361 aaatatcggt ataagtctta ctagatacga gatcatgatg agactgaaag aagaaaatcc
      421 attgctacaa agcagtttgc aagaaaagtt gcagattgat caaggagcag ttactcgtca
      481 tctgaaggta ttggaagcaa atggctatgt ttcgcgtcag agaaatcctc agaacaaccg
      541 ggaagtgtat gtagcgctaa cagaaaaagc ccagaagaaa atcgagcatt gtgaaatgga
      601 ccataaagat acaggttccg tgctatcccc ggctttcagc gatgtagaaa tgaaacaatt
      661 tctaacactt ttagataaat tgaataccca actttgcaat ataacaacag aaacgaaaga
      721 ggttaataat aatggaacaa aaaaatgatt tttcaacaat tatgactggt agaagatccg
      781 ttcgcgtata tgatgaaaca tacaaaatcc cacacgaaga aatgctggaa atgatcaaag
      841 aagcgacgac tgcaccttct tctgtcaata tgcaaccatg gcgctttgtg gtcgctgaaa
      901 gcgaggaagc aaaagaaatc ttgcgtcctt tgatccgttt caatacgaga caaaatgata
      961 cttcttctgc aatggtcatg atttttggcg atatgcaatg ttacgaatat ggcgaagaaa
     1021 tctataacca agcttacgaa tcagggaaga tgccaaaaga agtcagagat caacaattag
     1081 ctgcgatcat tccttattac aagagttttt caagagaaga aatgaacgat gtagtcaaag
     1141 tcgattctag cttagcggct atgcaattca tgttagttgc gcgtgatcat ggctatgaaa
     1201 caaatccaat tggtggattc gaagcagatc agttggcaga ggcatttggt ttggataaag
     1261 accgttatgt tccagttatt attttatcaa taggaaaagc ggtagaagaa ggttacgagt
     1321 ctgttcgttt agcaccagaa aagatcacta cgttcgaata aaaggttttc agttactatt
     1381 cagtagctat tgcttttttt gaatcgtgtg tggtaaccta ataaaaaagg aggtcggaaa
     1441 aatgactgca tcgatgaaat tacgtttcga acttttgaat aacaactaaa taaattcaaa
     1501 agacatgtat aaacaacaat ttcatggatg gagtatgtcc attttttcga acgacagtag
     1561 aataggtgaa agctatgaca gatttggtga tttagtcata gttatttcgt tcagagtagg
     1621 aaaagatgtg atgcagtttt tttgcaaaca gatttttctg ctctttttta gtatcgataa
     1681 ttgttgtttt acatgaaata ggagtgtaaa acatatggaa aatttagcag taaatataac
     1741 aaatctacaa gtcagctttg gcaaccagct agaactatct attgattctc ttcgtgtcta
     1801 tcagcaagat cgtataggga tcataggaga aaatggtgtc ggtaaatcga ctttgctcaa
     1861 actaatagcc ggtgaacttt ttcccgatca tgggaaaatc caaacagaga tcaccttcaa
     1921 ctacctgcct caattaacct atctcgctga agcaaaggac ctaaatttgg aattagccag
     1981 tcatttccag ttaagactgg aagaaacttc agagcggaaa tggagcggag gggaagaacg
     2041 aaagatcgag ttgatacgtc ttctttcttc ttatgaacaa gggatgcttc tagacgagcc
     2101 aacaacccat ctagatagaa aaagtattga tcgactgatt gaagagcttc gttattatta
     2161 tggtacgctg gtttttgtta gtcatgatcg ctattttcta gatgagctag catcgaaaat
     2221 ctgggaagta aaagacggag aaatccgaga gttttcgggg aattatagtg cctatctcac
     2281 tcaaaaggaa ttggagaaaa agactcagct acgagaagca gagtcgatca tgaaagagaa
     2341 aaaacgattg gaaaaatcga tccaagaaaa gaaaaaacaa gcggaaaagt tggaaaaagt
     2401 gtccagtaaa aagaaaaagc aacaaatcag accggatcgg ttgtcttcct ctaaacaaaa
     2461 agacagtgta caaaaagcca tccaaaagaa tgcgaaaaca ttagagagaa gactccaaaa
     2521 aataggagaa acaaccaagc cgcagcagat gaaacaaatc cgttttccag taccaaaatc
     2581 tcttgaactc cacagccgct atcccatcat gggacaaaat gtccaattgg aaagaagtgg
     2641 gagaacatta ctggtaaatg gtgattttca gttttcttta ggtaaaaaaa tcgcgattgt
     2701 cggtgaaaat ggttcaggta agacaacctt attagaacat atccgcaaac aaggagaagg
     2761 aatccttctc tctccgaaag taagctttca agtatatcag caaaagggtt atcaaatgac
     2821 atctgaagaa tccatcattc gttttgtcat gagacaaaca gagttttcag aatcgcttgt
     2881 ccgtagtttg ctgaatcact tagggtttgc tcaggaaact ttgacgaaac cgttatgtac
     2941 attaagtggg ggagaagcga cccgtctgac gattgctttg ctttttacta agccaagtaa
     3001 tgtgttgctg ttagatgaac cgactaattt tattgatatg gcaacgatcg aagctttaga
     3061 gaagctaatg caaatatatc cgggaacgat tttgtttact tctcatgatt cttactttgt
     3121 cgagcgtacg gctgatgaag tttatgaaat aaaagggcag aaaataaaaa aagtacttac
     3181 gagaaatttt taaatgtgct acgatgattt tcgtgacaaa tttaaaggag tggtacagat
     3241 tgcctacaaa taaaaaagaa ggaataattt ttacaacaat gatgtgtttt ttgatggtct
     3301 tggggatgag cgcgtataat ttatggctgc atcaagatct tcatttatca agtttgttga
     3361 ttggtctagt gcctggattc gtggtggctt tcattttgga tgtcttcatc atcggtgtgc
     3421 tggcgaaaaa aattgccttt aaactaccag taaatccaga aagcaaggtc caaatgatct
     3481 taactatttc atgcttgatg gttttaggaa tggtgacatg tatgtcttta ttcggtgtga
     3541 tcatggaaca aggaatacct gatcaattat tctctgctta tatgaatgct tggaaaatga
     3601 atgtgattgc cgctttaccg ttgcagttga tcatcgttgg cccgctttca agaagtgtgc
     3661 taaaagcaat tcaaagtaat gaatgatgga acactgttat ttgaaaaaag gccttggcat
     3721 gctttttgtc atagtctctt gaatgcagtt ctagaaaaat agtgatccga acgataggtt
     3781 ttggcctgtc gttcggatcg ctttttcttt tccaactgta ttgctctttt attgaagata
     3841 atttgctctt caatagatat ttctagaaag gttctgttta tcccttactc aaaaaactgc
     3901 tgcatttgtt ctcttacttg ctgacaccat tggagttcct gctgacaaag cataattctg
     3961 cgctggatca caacataacg tccaaattct tgtttcttgt ctagcgagag gtcattcaat
     4021 tttttttgat ttttttgcat gattttgttc agctgttgtt ctctagtgaa tagcagtcca
     4081 gtagctgttg ttttatcgag cgtcgaaaaa gcatagattt ttgctagaaa ttcatctttt
     4141 tgcgtaggag cgggagtttc ctctttgatc cattcctcta caagagcacg tccttcatca
     4201 gtcaagtggt agatcttttt ttgagagtgc tcgtctttgt ccaaaacggt cacataaccc
     4261 tgctggtcta acttg
//
LOCUS       DAMEET010000271         3278 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.guided.155, whole genome shotgun sequence.
ACCESSION   DAMEET010000271 DAMEET010000000
VERSION     DAMEET010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.guided.155"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..188
                     /locus_tag="PB780_002811"
     CDS             <1..188
                     /locus_tag="PB780_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDE0734774.1"
                     /translation="MNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKF
                     VQAFILPDGEEIKISNE"
     gene            203..1072
                     /locus_tag="PB780_002812"
     CDS             203..1072
                     /locus_tag="PB780_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDE0734775.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1053..1787
                     /locus_tag="PB780_002813"
     CDS             1053..1787
                     /locus_tag="PB780_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDE0734776.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1820..2728
                     /locus_tag="PB780_002814"
     CDS             1820..2728
                     /locus_tag="PB780_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HDE0734777.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2725..3205
                     /locus_tag="PB780_002815"
                     /pseudo
     CDS             2725..3205
                     /locus_tag="PB780_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1146 a          465 c          718 g          949 t
ORIGIN      
        1 aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttca
       61 ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc
      121 taggtgtcaa gtttgtgcag gcatttattt taccagatgg agaagaaata aaaatatcta
      181 acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt
      241 atcatctttg ccttttatag aaggcatcgt attagggggc tcacgtgcaa gaggcaccca
      301 tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac
      361 agcgattaat caaattgcta cagaattgga tgataaaaat agaaacaacc ttgttgtgcc
      421 tcccggagcg tggggtgatt ggattaatgg cggcggatgg ttatttataa atgggtatca
      481 tgttgacttg attttacgtg atataaaacg ggtagaacaa ataatcaaag atacggagca
      541 aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa gtgcaatgta
      601 tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt
      661 aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat
      721 atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggtag aggataaata
      781 ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac tatttgcatg
      841 taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa cttttgaata
      901 taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg gtctttctct
      961 ttttgaatgc tacgatatga ccgagaaact ttataaagaa gtgaatgaaa ttgtatcgga
     1021 gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata
     1081 tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag
     1141 aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag
     1201 gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag
     1261 gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga
     1321 ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa
     1381 tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat
     1441 ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg
     1501 ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg
     1561 acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat
     1621 atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc
     1681 agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa
     1741 tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa
     1801 ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt
     1861 agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa
     1921 tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc
     1981 gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc
     2041 ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga
     2101 tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa
     2161 gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt
     2221 tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg
     2281 caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt
     2341 atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg
     2401 gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga
     2461 aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta
     2521 tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga
     2581 ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac
     2641 cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc
     2701 tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat
     2761 atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac
     2821 gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac
     2881 gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag
     2941 ataatatatc ttttatatag aagatatcgc cgtatgtaag gatttcaggg ggcaaggcat
     3001 aggcagcgcg cttatcaata tatctataga atgggcaaag cataaaaact tgcatggact
     3061 aatgcttgaa acccaggaca ataaccttat agcttgtaaa ttctatcata attgtggttt
     3121 caaaatcggc tccgtcgata ctatgttata cgccaacttt gaaaacaact ttgaaaaagc
     3181 tgttttctgg tatttaaggt tttagaatgc aaggaacagt gaattggagt tcgtcttgtg
     3241 gttctgttgc aaagttttaa ataaagaata aaatccct
//
LOCUS       DAMEET010000272         3151 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.guided.157, whole genome shotgun sequence.
ACCESSION   DAMEET010000272 DAMEET010000000
VERSION     DAMEET010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.guided.157"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            260..1171
                     /locus_tag="PB780_002816"
     CDS             260..1171
                     /locus_tag="PB780_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HDE0734778.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1324..1809
                     /gene="vanZ"
                     /locus_tag="PB780_002817"
     CDS             1324..1809
                     /gene="vanZ"
                     /locus_tag="PB780_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HDE0734779.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            2328..2612
                     /locus_tag="PB780_002818"
     CDS             2328..2612
                     /locus_tag="PB780_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDE0734780.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
BASE COUNT         1169 a          446 c          582 g          954 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaaccc ccattgtcaa caggcagttc
       61 agcctcgtta aattcagcat gggtatcact tatgaaaatt catctacatt ggtgataata
      121 gtaaatccag tagggcgaaa taattgactg taatttacgg ggcaaaacgg cacaatctca
      181 aacgagattg tgccgtttaa ggggaagatt ctagaaatat ttcatacttc caactatata
      241 gttaaggagg agactgaaaa tgaagaagtt gtttttttta ttgttattgt tattcttaat
      301 atacttaggt tatgactacg ttaatgaagc actgttttct caggaaaaag tcgaatttca
      361 aaattatgat caaaatccca aagaacattt agaaaatagt gggacttctg aaaataccca
      421 agagaaaaca attacagaag aacaggttta tcaaggaaat ctgctattaa tcaatagtaa
      481 atatcctgtt cgccaagaaa gtgtgaagtc agatatcgtg aatttatcta aacatgacga
      541 attaataaat ggatacgggt tgcttgatag taatatttat atgtcaaaag aaatagcaca
      601 aaaattttca gagatggtca atgatgctgt aaagggtggc gttagtcatt ttattattaa
      661 tagtggctat cgagactttg atgagcaaag tgtgctttac caagaaatgg gggctgagta
      721 tgccttacca gcaggttata gtgagcataa ttcaggttta tcactagatg taggatcaag
      781 cttgacgaaa atggaacgag cccctgaagg aaagtggata gaagaaaatg cttggaaata
      841 cgggttcatt ttacgttatc cagaggacaa aacagagtta acaggaattc aatatgaacc
      901 atggcatatt cgctatgttg gtttaccaca tagtgcgatt atgaaagaaa agaatttcgt
      961 tctcgaggaa tatatggatt acctaaaaga agaaaaaacc atttctgtta gtgtaaatgg
     1021 ggaaaaatat gagatctttt attatcctgt tactaaaaat accaccattc atgtgccgac
     1081 taatcttcgt tatgagatat caggaaacaa tatagacggt gtaattgtga cagtgtttcc
     1141 cggatcaaca catactaatt caaggaggta aggatggcgg aatgaaacca acgaaattaa
     1201 tgaacagcat tattgtacta gcacttttgg ggtaacgtta gctttttaat ttaaaaccca
     1261 cgttaactag gacattgcta tactaatgat acaacttaaa caaaagaatt agaggaaatt
     1321 atattgggaa aaatattatc tagaggattg ctagctttat atttagtgac actaatctgg
     1381 ttagtgttat tcaaattaca atacaatatt ttatcagtat ttaattatca tcaaagaagt
     1441 cttaacttga ctccatttac tgctactggg aatttcagag agatgataga taatgttata
     1501 atctttattc catttggctt gcttttgaat gtcaatttta aagaaatcgg atttttacct
     1561 aagtttgctt ttgtactggt tttaagtctt acttttgaaa taattcaatt tatcttcgct
     1621 attggagcga cagacataac agatgtaatt acaaatactg ttggaggctt tcttggactg
     1681 aaattatatg gtttaagcaa taagcatatg aatcaaaaaa aattagacag agttattatt
     1741 tttgtaggta tacttttgct cgtattattg ctcgtttacc gtacccattt aagaataaat
     1801 tacgtgtaag atgtctaaat caagcaatct gatctttcat acacataaag atattgaatg
     1861 aattggatta gatggaaaac gggatgtggg gaaactcgcc cgtaggtgtg aagtgagggg
     1921 aaaaccggtg ataaagtaaa aagcttacct aacactatag taacaaagaa agcccaatta
     1981 tcaattttag tgctgaggaa ttggtctctt taataaattt ccttaacgtt gtaaatccgc
     2041 attttcctga cggtaccccc actagcagcc atgctttttt tgaatctttg taggacgttt
     2101 taaagagaat tacccacttt aaaattaaac atgcgcttag aatcgcttta ggaaacacga
     2161 tccagtccaa taatcgtcga taaaaacttt tgaaaaaggt tggtgaaatt acctactttt
     2221 ggaataatca caaatcacaa gtgattaatc acttgtttat taagatatta aaagctataa
     2281 tttaaataaa gcgtgaattt tattacacaa aaagaggggg gagaaacttg gaactagcat
     2341 ttagagaaag cttaaaaaag atgagaggta ccaaatcaaa agaaaaattc tcccaagaat
     2401 tagaaatgag tagatcaaat tattcacgaa tagaatcagg aaaatcagat ccaaccataa
     2461 aaacactaga acaaattgca aagttaacta actcaacgct agtagtggat ttaatcccaa
     2521 atgagccaac agaaccagaa ccagaaacag aatcagaaca agtaacattg gatttagaaa
     2581 tggaagaaga aaaaagcaat gacttcgtgt gaataatgca cgaaatcgtt gcttattttt
     2641 tttaaaagcg gtatactaga tataacgaaa caacgaactg aatagaaacg aaaaaagagc
     2701 catgacacat ttataaaatg tttgacgaca ttttataaat gcatagcccg ataagattgc
     2761 caaaccaacg cttatcagtt agtcagatga actcttccct cgtaagaagt tatttaatta
     2821 actttgtttg aagacggtat ataaccgtac tatcattata tagggaaatc agagagtttt
     2881 caagtatcta agctactgaa tttaagaatt gttaagcaat caatcggaaa tcgtttgatt
     2941 gctttttttg tattcattta tagaaggtgg agtttgtatg aatcatgatg aatgtaaaac
     3001 ttatataaaa aatagtttat tggagataag aaaattagca aatatctata cactagaaac
     3061 gtttaagaaa gagttagaaa agagaaatat ctacttagaa acaaaatcag ataagtattt
     3121 ttcttcggag ggggaagatt atatatataa g
//
LOCUS       DAMEET010000273         2303 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.guided.168, whole genome shotgun sequence.
ACCESSION   DAMEET010000273 DAMEET010000000
VERSION     DAMEET010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.guided.168"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1035)
                     /locus_tag="PB780_002819"
     CDS             complement(<1..1035)
                     /locus_tag="PB780_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDE0734781.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQ"
     gene            1588..2136
                     /gene="aac(6')"
                     /locus_tag="PB780_002820"
     CDS             1588..2136
                     /gene="aac(6')"
                     /locus_tag="PB780_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HDE0734782.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          738 a          406 c          451 g          708 t
ORIGIN      
        1 ctgtttaaat ttttctcggg tgatttcata aggcacatgt ttaatatcat ttgttgccat
       61 ggctttatct aagattgctt ctaaatcggt cgtgacattg agattcatgt cggttaacga
      121 tactggaagt tggttctttt tcataaacac atagatttta tcaaacaaat cgaactgctg
      181 atccatcatc aataaggtca gtacaccata actgacagat gctccgtgaa ggtatccctc
      241 cattttttct agaacagtcg taccgtaata gaatgagtgt gccatagcag aattatagtt
      301 attgttaact aaaacagaaa ccaatccggt agtaacaatg atatccgaaa cgacttgttc
      361 aattgcatat gagggacgat cgtcggcact atctttcaat gcttgcgtac cataagtcaa
      421 gagacgatca atgcacattt ttccgatttg gattcctaat ctatccgtgt agcctagatc
      481 atcgccacga gcagaaaaag tagattcgac ttctttggat aaagcatcgc caatacctgc
      541 ccataagtat ttatcaggtg cttcggcaat aattgctgta tcaatgaagg tgtgtacagg
      601 tggttttgaa ggaaagaaga gtccgtccat tacgccttct tcgttataga tgacacatac
      661 cgcagtgacg ggcgaacagt tcgaaccaat agtgggaaag gtgaagactg gtttagctag
      721 tttttcacca atgactttta cagtatcgca agcacggcca ccaccgactg caaacaggac
      781 gtctgctttt tggacacttt catgctggat caactgttca atatttccat aagttgaatt
      841 acccccgtac cagagactgg cgacaacctc tatttcctgt gtcaaagcta cttttatttt
      901 gttttctgcc tttgaaagtg cagtcttccc tccaatcagc actacttttg tcccatattc
      961 tttgacgatt ccatttgctt tttcatacgc atctgttcca atggtataat ttggtaagaa
     1021 aacactttga ctcatattca tttccttctt tcttcgttga aacgggttaa aattttaaag
     1081 agtgatcgat ctgactgcgg ctgtagatgg agtagaggtg aacctaagac aggataagtg
     1141 atgaataagg tggcatgaac accttgaaat catcaataag tatcctaaaa acgaacaagt
     1201 acaaaaacat tgaaaacgac aataatgaat ttttgttcct cttagtattt ctaccgattc
     1261 cctttgcttt tttgtaatta tctaaaaatt ctaaaaatta agattattat atgaaaacag
     1321 gttttatttg tcaattcata attggttttt tttaaagcaa aagataatct atatcatgat
     1381 gaacaaacag agttttttcc tccaattgat ggctttcctg atctttccta ttttgaataa
     1441 agtgtttctg tataattttt ttattttata agtaagataa ataatgaaag aatagagata
     1501 ctgattggta gctaaagatt tttgagcgag aacatttcgt tttttcaaaa ggactgttag
     1561 aataagcaag aacaaggggg aagacgtatg ataatcagtg aatttgaccg taataatcca
     1621 gtattgaaag atcagctttc tgatttactg agactgactt ggccggaaga atatggagac
     1681 agctcggcag aagaagtaga agaaatgatg aatccagaac gaatcgcggt agcagcggta
     1741 gaccaagatg agttagtagg atttattggt gcaatccctc aatacggtat cacaggttgg
     1801 gaattgcatc cattagttgt agaaagctcc cgacgaaaga accaaatagg tactcgatta
     1861 gtcaattact tagaaaaaga agtagcttcc agaggaggaa tcacgattta tttaggtacg
     1921 gatgatttag accatggaac aacgttaagt caaacggacc tgtatgagca tacatttgat
     1981 aaagtggctt ccatccagaa ccttcgtgaa catccgtatg aattctatga aaaattaggt
     2041 tataaaatcg taggtgtctt accaaatgca aatggctggg acaaaccgga tatttggatg
     2101 gcaaaaacga ttattcctcg accaaattct caataataaa aagaggttgt gacagaagta
     2161 atcagcttca agaaataaga aggaatttac gaaaattact tcttgaattt ttgtgaaatt
     2221 ccaacttatt tccgaagaag ctacttttgg aacaccgtgt accagaggtt gtgaaaaagt
     2281 cgcttagttt cgagtagtaa gaa
//
LOCUS       DAMEET010000274         1880 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.guided.180, whole genome shotgun sequence.
ACCESSION   DAMEET010000274 DAMEET010000000
VERSION     DAMEET010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.guided.180"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            22..1851
                     /gene="tet(M)"
                     /locus_tag="PB780_002821"
                     /pseudo
     CDS             22..1851
                     /gene="tet(M)"
                     /locus_tag="PB780_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
BASE COUNT          651 a          293 c          392 g          544 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggtt ctgttgcaaa
     1861 gttttaaata aagaataaaa
//
LOCUS       DAMEET010000275         1880 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.guided.181, whole genome shotgun sequence.
ACCESSION   DAMEET010000275 DAMEET010000000
VERSION     DAMEET010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.guided.181"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            22..1851
                     /gene="tet(M)"
                     /locus_tag="PB780_002822"
                     /pseudo
     CDS             22..1851
                     /gene="tet(M)"
                     /locus_tag="PB780_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
BASE COUNT          650 a          293 c          393 g          544 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggtt ctgttgcaaa
     1861 gttttaaata aagaataaaa
//
LOCUS       DAMEET010000276         1353 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.guided.205, whole genome shotgun sequence.
ACCESSION   DAMEET010000276 DAMEET010000000
VERSION     DAMEET010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.guided.205"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            48..923
                     /locus_tag="PB780_002823"
                     /note="aph(2'')-Ia"
     CDS             48..923
                     /locus_tag="PB780_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HDE0734783.1"
                     /translation="MKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTN
                     KKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTM
                     SEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEK
                     DYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDF
                     IYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEF
                     IENGRKEIYKRTYKD"
     gene            complement(1053..>1353)
                     /locus_tag="PB780_002824"
     CDS             complement(1053..>1353)
                     /locus_tag="PB780_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDE0734784.1"
                     /translation="LFDSRFKTLQQNQPKYSKACASLDDGFEDAFQYTVQGNSHNRLK
                     STNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          533 a          124 c          229 g          467 t
ORIGIN      
        1 atttaatgga atatagatat gatgataatg ccacaaatgt taaggcaatg aaatatttaa
       61 ttgagcatta ctttgataat ttcaaagtag atagtattga aataatcggt agtggttatg
      121 atagtgtggc atatttagtt aataatgaat acatttttaa aacaaaattt agtactaata
      181 agaaaaaagg ttatgcaaaa gaaaaagcaa tatataattt tttaaataca aatttagaaa
      241 ctaatgtaaa aattcctaat attgaatatt cgtatattag tgatgaatta tctatactag
      301 gttataaaga aattaaagga acttttttaa caccagaaat ttattctact atgtcagaag
      361 aagaacaaaa tttgttaaaa cgagatattg ccagtttttt aagacaaatg cacggtttag
      421 attatacaga tattagtgaa tgtactattg ataataaaca aaatgtatta gaagagtata
      481 tattgttgcg tgaaactatt tataatgatt taactgatat agaaaaagat tatatagaaa
      541 gttttatgga aagactaaat gcaacaacag tttttgaggg taaaaagtgt ttatgccata
      601 atgattttag ttgtaatcat ctattgttag atggcaataa tagattaact ggaataattg
      661 attttggaga ttctggaatt atagatgaat attgtgattt tatatactta cttgaagata
      721 gtgaagaaga aataggaaca aattttggag aagatatatt aagaatgtat ggaaatatag
      781 atattgagaa agcaaaagaa tatcaagata tagttgaaga atattatcct attgaaacta
      841 ttgtttatgg aattaaaaat attaaacagg aatttatcga aaatggtaga aaagaaattt
      901 ataaaaggac ttataaagat tgattatata atatatgaaa agctattata aaagacatta
      961 gtattaaata gtttaaaaaa atgaaaaata ataaaggaag tgagtcaatt ccagactcct
     1021 gtgtaaaatg ctatacaatg tttttaccat ttctacttat caaaattgat gtattttctt
     1081 gaagaataaa tccattcatc atgtaggtcc ataagaacgg ctccaattaa gcgattggct
     1141 gatgtttgat tggggaagat gcgaataatc ttttctcttc tgcgtacttc ttgattcagt
     1201 cgttcaatta gattggtact ctttagtcga ttgtgggaat ttccttgtac ggtatattga
     1261 aaggcgtctt cgaatccatc atccaatgat gcgcaagctt ttgaatattt tggttggttc
     1321 tgttgcaaag ttttaaatct actatcaaat aag
//
LOCUS       DAMEET010000277         1149 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.guided.216, whole genome shotgun sequence.
ACCESSION   DAMEET010000277 DAMEET010000000
VERSION     DAMEET010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.guided.216"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            12..77
                     /locus_tag="PB780_002825"
     CDS             12..77
                     /locus_tag="PB780_002825"
                     /inference="COORDINATES: protein motif:HMM:NF018061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HDE0734785.1"
                     /translation="MFYTIIWTLSKQTKNSDYADK"
     gene            202..939
                     /gene="erm(B)"
                     /locus_tag="PB780_002826"
     CDS             202..939
                     /gene="erm(B)"
                     /locus_tag="PB780_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDE0734786.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          421 a          200 c          185 g          343 t
ORIGIN      
        1 catggccttt tatcttttac acaattatat ggactttatc gaaacagact aaaaacagtg
       61 attacgcaga taaataaata cgttagatta attcctacca gtgactaatc ttatgacttt
      121 ttaaacagat aactaaaatt acaaacaaat cgtttaactt ctgtatttgt ttatagatgt
      181 aatcacttca ggagagatta catgaacaaa aatataaaat attctcaaaa ctttttaacg
      241 agtgaaaaag tactcaacca aataataaaa caattgaatt taaaagaaac cgataccgtt
      301 tacgaaattg gaacaggtaa agggcattta acgacgaaac tggctaaaat aagtaaacag
      361 gtaacgtcta ttgaattaga cagtcatcta ttcaacttat cgtcagaaaa attaaaactg
      421 aatactcgtg tcactttaat tcaccaagat attctacagt ttcaattccc taacaaacag
      481 aggtataaaa ttgttgggaa tattccttac catttaagca cacaaattat taaaaaagtg
      541 gtttttgaaa gccgtgcgtc tgacatctat ctgattgttg aagaaggatt ctacaagcgt
      601 accttggata ttcaccgaac actagggttg ctcttgcaca ctcaagtctc gattcagcaa
      661 ttgcttaagc tgccagcgga atgctttcat cctaaaccaa aagtaaacag tgtcttaata
      721 aaacttaccc gccataccac agatgttcca gataaatatt ggaagctata tacgtacttt
      781 gtttcaaaat gggtcaatcg agaatatcgt caactgttta ctaaaaatca gtttcatcaa
      841 gcaatgaaac acgccaaagt aaacaattta agtaccatta cttatgagca agtattgtct
      901 atttttaata gttatctatt atttaacggg aggaaataat tctatgagtc gcttttttaa
      961 atttggaaag ttacacgtta ctaaagggaa tggagataaa ttattagata tactactgac
     1021 agcttccaag aagctaaaga ggtccctagc gcctacgggg aatttgtatc gataaggggt
     1081 acaaattccc actaagcgct cgggacccct tgtaggaaaa tgtcctaagt gtggcaacaa
     1141 tattgtatt
//
LOCUS       DAMEET010000278          973 bp    DNA     linear   BCT 09-JAN-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VD165
            SAMN28857435-rid16559923.guided.226, whole genome shotgun sequence.
ACCESSION   DAMEET010000278 DAMEET010000000
VERSION     DAMEET010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28857435
            Sequence Read Archive: SRR19537087
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001557442.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001557442.1
            Genome Coverage       :: 33x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/03/2023 22:23:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,772
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 33 of 72
            Pseudo Genes (incomplete)         :: 32 of 72
            Pseudo Genes (internal stop)      :: 18 of 72
            Pseudo Genes (multiple problems)  :: 11 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28857435-rid16559923.guided.226"
                     /isolate="VD165"
                     /isolation_source="Rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2015-08-07"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            24..416
                     /locus_tag="PB780_002827"
     CDS             24..416
                     /locus_tag="PB780_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDE0734787.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            417..>973
                     /gene="aac(6')-I"
                     /locus_tag="PB780_002828"
     CDS             417..>973
                     /gene="aac(6')-I"
                     /locus_tag="PB780_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(6')-Ie family aminoglycoside
                     N-acetyltransferase"
                     /protein_id="HDE0734788.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNV"
BASE COUNT          393 a           79 c          169 g          332 t
ORIGIN      
        1 tatagttgaa gaatattatc ctattgaaac tattgtttat tgatgataat atggtttttg
       61 atattgataa tttaaaagga tttcttaatg ataccagttc atttgggttt atagctaaag
      121 aaaataataa aattatagga tttgcatatt gctatacact tttaagacct gatggaaaaa
      181 caatgtttta tttacactca ataggaatgt tacctaacta tcaagacaaa ggttatggtt
      241 caaaattatt atcttttatt aaggaatatt ctaaagagat tggttgttct gaaatgtttt
      301 taataactga taaaggtaat cctagagctt gccatgtata tgaaaaatta ggtggtaaaa
      361 atgattataa agatgaaata gtatatgtat atgattatga aaaaggtgat aaataaatga
      421 atatagttga aaatgaaata tgtataagaa ctttaataga tgatgatttt cctttgatgt
      481 taaaatggtt aactgatgaa agagtattag aattttatga tggtagagat aaaaaatata
      541 cattagaatc attaaaaaaa cattatacag agccttggga agatgaagtt tttagagtaa
      601 ttattgaata taacaatgtt cctattggat atggacaaat atataaaatg tatgatgagt
      661 tatatactga ttatcattat ccaaaaactg atgagatagt ctatggtatg gatcaattta
      721 taggagagcc aaattattgg agtaaaggaa ttggtacaag atatattaaa ttgatttttg
      781 aatttttgaa aaaagaaaga aatgctaatg cagttatttt agaccctcat aaaaataatc
      841 caagagcaat aagggcatac caaaaatctg gttttagaat tattgaagat ttgccagaac
      901 atgaattaca cgagggcaaa aaagaagatt gttatttaat ggaatataga tatgatgata
      961 atgccacaaa tgt
//
